LOCUS       DASPYY010000001         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1123612, whole genome shotgun sequence.
ACCESSION   DASPYY010000001 DASPYY010000000
VERSION     DASPYY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123612"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VEP73_00005"
     CDS             complement(<1..1324)
                     /locus_tag="VEP73_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYT24853.1"
                     /translation="MILSPPSQAPPLPAGPTPAVLDGVLERVTYANEETGYTVARVAS
                     PGSADLITVVGPLLGAQPGGRLRLEGRWRSHPLYGRQFEVRSYSTLLPATVEGIRRYL
                     GSGLIKGIGPKMAERIVDHFGTDTLRVLEEDPGRLHGVPGLGPKRTAMIEQAWQEQKS
                     IKEVMVFLQGVGVSTSLAVRIYRLYRDEAVEVVSKEPYRLAADVWGIGFKTADRIAQG
                     LGIGYDSPERIKAGLRFTLSEASETGHCYLPEPELVAKASEILGVRPTLTEACLEELV
                     RERGVVRDPVPIPGFDDGVAAVYLVPFHRAEVSLAAGLLRLRSAPPAADRLPAFQAVD
                     WTVAFDWLHRVTGYQLATEQAAAVRLALTERVAVLTGGPGCGKSFAVRAVVTLARAKQ
                     ARVVLAAPTGRAAKRLSELAGMQAQTLHRLLDLRPGGDAGFDHERPLDA"
     gene            complement(1390..1827)
                     /gene="ssb"
                     /locus_tag="VEP73_00010"
     CDS             complement(1390..1827)
                     /gene="ssb"
                     /locus_tag="VEP73_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYT24854.1"
                     /translation="MSDNQVTLVGNLTDDPELRSTPTGLPVANFRLAVSQRLWDADGW
                     KDGEPSFFRVNVWRDQAEHVNRSLRKGARCVVVGRLKSRSWETPEGQRRSVVEVEADE
                     VAASLRFAPATTAHAAGKAEGEGNGRHAAAPVAAGAALDEVPF"
BASE COUNT          254 a          772 c          771 g          295 t
ORIGIN      
        1 ccgcgtccag cggccgctcg tggtcgaagc cggcgtcccc gcccggccgc aggtcgagca
       61 gccggtgcag ggtctgcgcc tgcatccccg ccagctcgga caggcgcttg gcggccctgc
      121 cggtcggcgc ggccagcacg accctggcct gcttggccct ggcgagcgtg acgacggcgc
      181 gtaccgcgaa gctcttgccg cagcccgggc cgccggtgag cacggcgacc cgctcggtca
      241 gggcgagacg gaccgccgcg gcctgctcgg tggcgagctg gtagccggtg acccggtgca
      301 gccagtcgaa cgccaccgtc cagtccaccg cctggaaggc ggggagccgg tcggccgcgg
      361 ggggcgccga gcgcaggcgc agcagccccg cggccagcga cacctccgcg cggtggaacg
      421 ggaccaggta gacggcggcc acgccgtcgt cgaaccccgg gatggggacc gggtcgcgga
      481 ccacgccgcg ctcgcgcacc agctcctcga ggcacgcctc ggtgagtgtc gggcgcacgc
      541 cgaggatctc ggacgccttg gcgaccagct ccggctcggg gaggtagcag tggccggtct
      601 cgctggcctc cgagagggtg aagcgcagcc cggccttgat ccgctccggg ctgtcgtagc
      661 cgatgccgag cccctgggcg atcctgtcgg cggtcttgaa gccgatgccc cacacgtccg
      721 ccgcgagccg gtagggctcc ttggagacga cctcgacggc ctcgtcccgg tacagccgat
      781 agatccgcac ggccagggag gtcgacaccc ccacgccctg caggaagacc atgacctcct
      841 tgatggactt ctgctcctgc caggcctgct cgatcatggc cgtgcgcttg gggccgagcc
      901 ccggcacgcc gtgcaggcgc ccggggtcct cctcgagcac gcggagggtg tcggtgccga
      961 agtggtcgac gatgcgttcg gccatcttgg ggccgatgcc cttgatcagg ccggagccga
     1021 ggtaacggcg gatgccctcc accgtggcgg ggagcagggt gctgtagctg cggacctcga
     1081 actggcggcc gtacagcgga tggctccgcc agcgtccctc caggcgcagg cgcccgcccg
     1141 gctgggcgcc gagcaggggc ccgaccacgg tgatcaggtc ggccgacccg gggctcgcca
     1201 cccgggcgac ggtgtagccg gtctcctcgt tggcgtaggt gacgcgctcc agcacgccgt
     1261 ccagcaccgc gggggtggga ccggccggaa ggggcggagc ttgggatggc ggggacaaga
     1321 tcacgggggc tcctcgaggt tcagggcccg gccgtggctc ggccgcggcg tgggctggcc
     1381 cgtgggcgcc tagaacggca cctcgtcgag tgctgcgccg gccgccacgg gcgcggcggc
     1441 gtgccggccg ttgccctcgc cctcggcctt cccggccgcg tgggccgtgg tggcgggcgc
     1501 gaaccgcagg ctcgcggcca cctcgtccgc ctcgacctcc acgaccgacc gacgctgccc
     1561 ctccggggtc tcccacgacc ggctcttgag gcggccgacg accacgcagc gggcgccctt
     1621 gcgcaggctc cggttgacgt gctccgcctg gtcccgccag acgttcaccc tgaagaacga
     1681 cggctcgccg tccttccagc cgtcggcgtc ccacagccgc tggctgacgg cgagccggaa
     1741 gttggccacc gggaggccgg tcggcgtgga ccgcagctcc gggtcgtcgg tcaggttgcc
     1801 cacgagggtg acctggttgt cgctcacctt cgaactcctt ctcctggttc ggcttgcctg
     1861 gttcggcttg cctggttcgg cttgcctgct tcagcgtagg tacattctac cctatgaatc
     1921 gaacatatgt tcgaacaacc tgccggccgc gaccgccgcc ccgggcgggg cgtgcccgtc
     1981 cggggcggtc cgcgggtcgc gggcggacgc cctatccggc ctgccgttcg tcctcggcct
     2041 gcccccgcat gtcccggacc ttggcccgga aggcggccac gccggagagc tc
//
LOCUS       DASPYY010000002         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605 k141_121,
            whole genome shotgun sequence.
ACCESSION   DASPYY010000002 DASPYY010000000
VERSION     DASPYY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VEP73_00015"
     CDS             <1..455
                     /locus_tag="VEP73_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HYT24855.1"
                     /translation="ELAIGAVAEGIEAVDHDAVRRLGLDPAAVRAEIARQREEVTRRT
                     AAYRGDRPPVALAGRTAVVVDDGVATGWTCVAAAGWCRRAGAGRVVLAVPVGPPRLAR
                     RLGAAVDEVVILETPERYVAVGQAFADFPQVTDEEVLDCLNRRERRRG"
     gene            524..>2021
                     /locus_tag="VEP73_00020"
     CDS             524..>2021
                     /locus_tag="VEP73_00020"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HYT24856.1"
                     /translation="MSSPPPQDLNLAARSIAFRPSEEELRAFADEMPQARQTEYGNVN
                     VRTKVLARSKGSTYIVTDRPGEHSDQTVSREEGARIAAVQDEYIAGRDMVVADGFIGH
                     AGEYRTPARLVIEKANANIAGMQRYLYYDPVDDGGTRHEPEITIVYTPNLTMPGYPDD
                     RVIAVDLEAGVTRVINSDYFGESKKGGLRMWNKRVYEGGGLAMHAGCKVIPTSDGRRT
                     MLIVGLSGTGKTTTTFTKQNGSQAVQDDFIGLFPGGAVVSTEDGCFAKTFALSAEDEP
                     AIHGAVTRPDAYLENVSQHGGEIDFFDESYTKNGRAVFNMRSIDNWPADQVPPASFLL
                     ILNRNDNIIPGVARLNIEQAAGYFMLGETQGTSAGGKAEEGKALRVPGTNPFFPLRHE
                     QQGNRLAELLETHPLEVFLLNTGRVGGPDGDDRSRKIRIPHSSAIVRGIAEGSITWDE
                     DPDFAYQVASSVPGVDDVDLLQPRRLYERQGRMDEYREQVERLKAERAE"
BASE COUNT          330 a          728 c          713 g          250 t
ORIGIN      
        1 ccgagctggc catcggcgcg gtcgccgagg gcatcgaggc ggtggaccac gacgcggtgc
       61 ggcggctggg gctcgacccg gcagcggtcc gggcggagat cgccaggcag cgggaggagg
      121 tcacccgccg cacggccgcc taccgcggcg accgcccgcc ggtcgcgctc gccgggcgga
      181 ccgccgtcgt cgtcgacgac ggcgtggcca ccggctggac gtgcgtcgcg gcggccggct
      241 ggtgccgccg ggccggcgcc ggccgggtgg tcctcgccgt gccggtcggg ccgccacggc
      301 tcgcgcgccg gctcggtgcg gcagtcgacg aggtggtgat cctggagacc ccggagcggt
      361 acgtcgcggt cggccaggcg ttcgccgatt tcccgcaggt caccgacgag gaagtgcttg
      421 actgtctcaa taggcgggaa cgacgtcgag gatagactgg tggacctgct ccggcgccaa
      481 cgccggtccg cagcaggccc accctgcacc ggaggcaccg cccatgtcga gcccgccccc
      541 acaggacctc aacctcgccg ccaggtcgat cgcgttccgc ccgagcgagg aggagctgcg
      601 ggcgttcgcc gacgagatgc cgcaggccag gcagacggag tacggcaacg tcaacgtcag
      661 gacgaaggtc ctcgcgcggt ccaagggctc gacctacatc gtcaccgacc ggcccgggga
      721 gcactccgac cagacggtca gccgcgagga gggcgcccgg atcgcggccg tccaggacga
      781 gtacatcgcc ggccgcgaca tggtcgtggc cgacggcttc atcggccacg ccggtgagta
      841 ccgcacgccg gctcgcctcg tcatcgagaa ggcgaacgcg aacatcgccg ggatgcagcg
      901 ctacctctac tacgacccgg tcgacgacgg cgggacgcgc cacgaaccgg agatcaccat
      961 cgtctacacc ccgaacctca ccatgcccgg ctacccggac gaccgggtga tcgcggtgga
     1021 cctcgaggcc ggggtgacgc gggtcatcaa ctccgactac ttcggggagt ccaagaaggg
     1081 cggcctgcgc atgtggaaca agcgggtcta cgagggcggc ggcctcgcca tgcacgcggg
     1141 ctgcaaggtg atccccacct cggacggccg caggacgatg ctgatcgtcg gcctgtccgg
     1201 caccggcaag acgaccacga cgttcacgaa gcagaacggc tcccaggcgg tccaggacga
     1261 cttcatcggc ctgttcccgg gcggggcggt ggtctccacc gaggacggct gcttcgccaa
     1321 gacgttcgcc ctctccgccg aggacgagcc ggccatccac ggcgcggtga ccaggccgga
     1381 cgcctacctc gagaacgtct cccagcacgg cggcgagatc gacttcttcg acgagtccta
     1441 caccaagaac ggccgcgcgg tgttcaacat gcgctcgatc gacaactggc cggccgacca
     1501 ggtgcccccg gcgtcgttcc tgctcatcct gaaccggaac gacaacatca tccctggcgt
     1561 cgccaggctg aacatcgagc aggcggccgg gtacttcatg ctcggcgaga cccagggcac
     1621 gtcggcgggc ggcaaggccg aggagggcaa ggcgctccgc gtcccgggca cgaacccgtt
     1681 cttcccgctc cgccacgagc agcagggcaa ccggctggcc gagctgctcg agacccaccc
     1741 actcgaggtg ttcctgctca acaccggtcg ggtcggcggc ccggacggcg acgaccgatc
     1801 caggaagatc cgcatcccgc actcgtcggc gatcgtgcgg ggcatcgccg agggctcgat
     1861 cacctgggac gaggaccccg acttcgccta ccaggtggcc tcctcggtcc cgggcgtcga
     1921 cgacgtcgac ctcctgcagc cgcgccggct gtacgagcgc caggggcgca tggacgagta
     1981 ccgcgagcag gtcgagcgcc tgaaggccga gcgggccgag t
//
LOCUS       DASPYY010000003         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1545306, whole genome shotgun sequence.
ACCESSION   DASPYY010000003 DASPYY010000000
VERSION     DASPYY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545306"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VEP73_00025"
     CDS             <1..635
                     /locus_tag="VEP73_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24857.1"
                     /translation="PPSPGFPDGRVLSTGGYHNAAAPAALHGLAVCWADLCQLAERHV
                     ERLVFTTTTTTTTTAAASSGTASDGSELLRLLMMVAVGYSEEARGAAQPVVLPRGGPG
                     QNDVASPAFLAWSRERAAAGCLEAVLALLAAAACHLLPTAGAGGDLTPRLAQLAVETR
                     ERFPPLVERRPFGVDAARLTDYFRALVFAQAADGSGRRQRREPAPTQAGD"
     gene            complement(719..1267)
                     /locus_tag="VEP73_00030"
     CDS             complement(719..1267)
                     /locus_tag="VEP73_00030"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24858.1"
                     /translation="MSETVEAIGYRADVPSRAKEAVTGKVEAVKEKVSDTATRAREAV
                     TGTASRVGDTVGDTASRVGDRTPDKQQAKRTARRAVGLAQENPLGLAIGAVALGFLAG
                     LAVPASRVENEKIGPLADQVKDKVKETGQEAFERGREVAQEAAQSAVQTARERGQEHG
                     QDLAESARRNAQDVQAEARSSF"
     gene            complement(1285..>1627)
                     /locus_tag="VEP73_00035"
     CDS             complement(1285..>1627)
                     /locus_tag="VEP73_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYT24859.1"
                     /translation="QLLRQEIELAKAEVQEKGRKAGAGAGLFGGAGVLGLLAAAALTA
                     CFILALSEVMHPALAALLVAVVYGAVAGILALRGRDKIREAGPPVPEQTIETVKEDVQ
                     WAKTQMPSAKK"
BASE COUNT          171 a          656 c          549 g          251 t
ORIGIN      
        1 tgccgccctc accgggcttc ccggacggcc gcgtgctcag cacgggcggc taccacaacg
       61 cggccgcgcc cgccgcgttg cacggcctcg ccgtctgctg ggcggacctg tgccagctcg
      121 ccgagcgcca cgtcgagcgg ctcgtcttca ccaccaccac caccaccacc accaccgccg
      181 ccgcgtcgtc cggcaccgcg tccgacggga gcgagctgct gcgcctgctc atgatggtcg
      241 cggtcggata ctccgaagag gcgcgcgggg cagcacagcc ggtcgtcctt ccgcgcgggg
      301 ggcccgggca gaacgacgtc gcctcgccgg cgttcctggc ctggtcacgg gagcgcgccg
      361 ccgctggctg cctcgaagcg gtgctcgcgc tgctcgccgc ggcggcctgc catctgctcc
      421 cgaccgccgg tgccggagga gacctgacgc cgcgactggc gcagctcgcc gtcgagaccc
      481 gcgagcgctt ccctccgctc gtcgagcggc ggccctttgg cgtcgatgcc gccaggctga
      541 cggactactt ccgcgcgctc gtgttcgcgc aggcagcgga cggcagcgga cggcggcagc
      601 gacgcgagcc ggctcccacc caggccgggg actgacggac cccccttgca ctccgaaagc
      661 ggcagcggct accggcaccc cggcctggac cggccggccg gggtgccggc tcgtccggtc
      721 agaagctcga gcgggcctcg gcctggacgt cctgggcgtt ccgcctggcg ctctcggcca
      781 ggtcctggcc atgctcctgc ccgcgctccc tggcggtctg cacggcgctc tgggccgcct
      841 cctgggcgac ctccctgccc cgctcgaacg cctcctggcc ggtctccttg accttgtcct
      901 tcacctggtc ggccagcggg ccgatcttct cgttctctac ccggctcgcc gggacggcca
      961 ggccggccag gaagccgagg gctaccgcgc cgatcgcgag cccgagcggg ttctcctggg
     1021 cgaggccgac ggcccgccgc gcggtccgct tggcctgctg cttgtcgggg gtccggtcgc
     1081 ccacccgcga ggcggtgtcg ccgacggtgt cgccgacgcg cgacgcggtc ccggtgaccg
     1141 cctccctggc gcgggtcgcg gtatcgctca ccttctcctt cacggcctcc acctttcccg
     1201 tcacggcctc cttggcccgt gacggcacgt ctgcccggta tccgatggcc tcgacggtct
     1261 cgctcatctc ggcccgcgtg cgttctattt cttggcggat ggcatctggg tctttgccca
     1321 ctgcacgtcc tccttcacgg tctcgatcgt ctgctcgggg accggcgggc ccgcctccct
     1381 gatcttgtcc ctgccccgga gggcgaggat cccggcgacc gcgccgtaca ccaccgcgac
     1441 cagcagcgcg gcgagtgcgg ggtgcatcac ctcgctgagc gcgaggatga agcaggcggt
     1501 cagcgcggca gcggcgagca gccctagcac gcccgcgcca ccgaacagcc cggccccggc
     1561 cccggccttc ctgcccttct cctgcacctc ggcctttgcg agctcgatct cctggcgcag
     1621 gagctgg
//
LOCUS       DASPYY010000004         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1826257, whole genome shotgun sequence.
ACCESSION   DASPYY010000004 DASPYY010000000
VERSION     DASPYY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826257"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="VEP73_00040"
     CDS             <1..1969
                     /locus_tag="VEP73_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYT24860.1"
                     /translation="HRVRRGTNQAMATVTRERAPTGGQPAADVLAALAAGILGPGCSP
                     EVPARVLSILDQLASRRDREQLLAILRLAGTRAGALALTGHRKPVAGLSPAEAEAVVQ
                     RWQASRLGVQRQLAAAVTAATLTACYAWPGPAWTRIGYPGPLGPAPDVPKRLHPIATA
                     PGEELACDVVVVGSGAGGGTVAAELARAGADVVVLEKGGYASESDFSHLEHEANRDLY
                     LYGLTLATTDLGCRILAGSTLGGGTVVNYSTSFKTPGFVLAEWAKVSGLDAFVSGEVE
                     ASLDAVAERLSVTTGASRPSPRDVLMERGLARLGWHCAPLPRNVRGCPQHTGCGWCGF
                     GCRLGAKQSTLRTYLEDAAARGARLVVGADVRRVLINDARAVGVEARVGGHRLRVRAK
                     AVVAAAGAIETPALLLRSGLTGQVGRHLHLHPGTGALGTFAQGVRPWEGTLQARYSAE
                     LRGTLGEYGPILETVPLHPGTGAVGMPWRSAADHRALMDRYASMSLCASLTRDTTSGR
                     VRIDRDGNPRVAYKLNGTDERHVAAGLAACGQVLAAAGATEVMSLHAERHSFRPGRPD
                     AFEAWADATRRAGAGGGRLTLFSYHQMGSCRMGTDPATSAVDGDHQTHQVERLFVTDS
                     SVFPTASGVNPMLSIMGLAHRAAGRITAHVG"
     gene            2519..>3207
                     /locus_tag="VEP73_00045"
     CDS             2519..>3207
                     /locus_tag="VEP73_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYT24861.1"
                     /translation="MDTNTSSATPPAAAPTRAADDLDALDRLLDRLTADDPTSRPDPA
                     AAERVLRLRRQANRFEAIWQRELAAVDGRGAAGAETGTPAPSTAGWLRARLRLDRGTA
                     SGQVRVARALHRGRLPATAAAALAGELSPQHTAVIADGLAGLDPATATAAEPVVLDAA
                     RRLDPAGLRRAADHLRSVADPQGEDQRAQARWARRGLRVSSTWQGLTALDGLLDPEQG
                     ETLLAALQPLAR"
BASE COUNT          379 a         1265 c         1199 g          364 t
ORIGIN      
        1 gcaccgggtc cggcggggta caaaccaggc catggcaacg gtgacacggg aacgggcccc
       61 cacgggcggg cagccggccg ccgacgtgct cgccgccctg gcagccggga tcctcggccc
      121 cggctgctcg cccgaggtcc ccgcgcgggt gctctccatc ctggaccagc tcgcctcgcg
      181 ccgcgaccgc gagcagctcc tcgcgatcct gcgcctcgcc ggcacccgcg cgggcgccct
      241 cgccctgacc ggccaccgca agccggtcgc cggcctgtct cccgccgagg ccgaggcggt
      301 ggtgcagcgc tggcaggcca gccgcctcgg ggtgcagcgg cagctcgccg cggcggtcac
      361 cgccgccacc ctgaccgcct gctacgcctg gccgggcccg gcctggaccc gcatcggcta
      421 cccggggccg ctcggccccg cgcccgacgt gccgaagcgc ctccacccga tcgcgaccgc
      481 cccgggggag gagctcgcct gcgacgtcgt cgtggtgggc tcgggggcgg ggggcggcac
      541 ggtggccgcc gagctggccc gggccggggc ggacgtggtc gtcctcgaga agggcggcta
      601 cgcgagcgag tcggacttct cccacctcga gcacgaggcc aaccgcgacc tctacctgta
      661 cgggctcacg ctcgccacga ccgacctcgg ctgccggatc ctggccgggt cgacgctcgg
      721 cggcggcacg gtcgtcaact actccacctc gttcaagacc ccgggcttcg tgctcgcgga
      781 gtgggcgaag gtctccgggc tggacgcctt cgtctccggc gaggtcgagg cgtcgttgga
      841 cgcggtcgcc gagcggctct cggtcaccac cggcgcgagc cggccgagcc cgcgggacgt
      901 gctcatggag cggggcctcg cgcggctcgg ctggcactgc gcgccgctgc cccgcaacgt
      961 ccgcggctgc ccgcagcaca ccggctgcgg ctggtgcggg ttcggctgcc ggctcggggc
     1021 caagcagtcc accctgcgga cctacctcga ggacgccgcg gcgcgcggcg ccaggctcgt
     1081 cgtgggggcg gacgtgcggc gggtgctcat caacgacgcc cgcgcggtgg gagtcgaggc
     1141 gcgcgtgggc ggccaccgcc tccgggtcag ggccaaggcg gtggtcgcgg ccgccggcgc
     1201 gatcgagaca cccgccctgc tgctgcgctc cgggctcacc ggccaggtcg gccggcacct
     1261 ccacctgcac ccgggcaccg gcgcgctcgg caccttcgcc cagggcgtgc gcccgtggga
     1321 gggcacgctc caggcgcgct actcggccga gctccgcggc accctcggcg agtacgggcc
     1381 gatcctcgag accgtgccgc tgcacccggg caccggggcg gtggggatgc cgtggcggtc
     1441 ggcggccgac caccgcgcgc tcatggaccg gtacgccagc atgtcgctgt gcgccagcct
     1501 cacccgcgac acgacgagcg ggcgggtgcg gatcgaccgg gacggcaacc cgcgggtcgc
     1561 ctacaagctg aacggcaccg acgagcgcca cgtcgcggcc ggcctggccg cctgcgggca
     1621 ggtgctcgcc gcggccgggg ccaccgaggt catgtccctg cacgccgagc gccattcgtt
     1681 ccgcccaggg cggccggacg ccttcgaggc gtgggcggac gcgaccaggc gggccggcgc
     1741 cggcggcggg aggctcaccc tgttctccta ccaccagatg ggctcctgcc ggatggggac
     1801 cgacccggcc acctcggccg ttgacggcga ccaccagacc caccaggtgg agcgcctctt
     1861 cgtgaccgac tcgtcggtct tcccgaccgc atcgggcgtc aacccgatgc tgtccatcat
     1921 gggcctcgcc caccgggccg ccggccgcat caccgcgcac gtgggctgac gcaacggggg
     1981 tggtcgacga cgacccgcaa ccgggccgcg agacgacgcc gacggcgacg gccgcagcgc
     2041 accgacgctt gaagcaacga cgatctgcgc gctcgtgcag ccgccgtcca ccccggcgcc
     2101 ggtgaccgcc acacgcaccg agttacgcgg cccgctgacc gcgagggaag cggtcgcgga
     2161 ccagcgccag gctgggcggc atgacaatcg ctgcgccgag ccttggacgg cgcggacccg
     2221 atggccggtg gttgcgagcc gcggaatgga ttccctggtc atcgctgcag tcggcggagc
     2281 ggccgatcct cggcaggtcc tggacctctg ttgccgcagc tggacgcgct cgtcgcagcg
     2341 cgtgcccgag gtcgtcagtg tgctgccagg caagccgcat gggctgcgta cgcggcagtg
     2401 gaccacccgt gttgcaggca gtcgccgggc gggagggaag tgccggccgg tgcggtggat
     2461 gtccacagac tgtggtggtg ggctgggcgg cgggtgtcgg cacggctagc ctccccccat
     2521 ggatacgaac accagttcgg ccaccccacc ggccgcagcg ccgaccaggg cggcggacga
     2581 cctggacgcg ctcgaccgcc tgctcgaccg gctcaccgcc gacgacccca ccagccggcc
     2641 cgacccggcc gccgccgagc gggtcctgcg gctgcgccgc caggccaacc ggttcgaggc
     2701 catctggcag cgggaactgg ccgctgtgga cggccgcggc gccgccggcg ccgagaccgg
     2761 caccccggcg ccctccaccg ccggatggct gcgcgcccgg ctgcgccttg accgggggac
     2821 cgccagcggg caggtgcggg tcgcccgcgc gctgcaccgc ggccggctgc ccgccaccgc
     2881 cgcggcggcg ctggccgggg agctgtcccc ccagcacacc gccgtcatcg ccgacggcct
     2941 ggccggcctc gacccggcca cggcaaccgc ggcggagccg gtggtgctgg acgccgcccg
     3001 ccgcctcgac cctgccgggc tgcgccgcgc cgccgaccac ctgcggtctg tcgccgaccc
     3061 ccagggggag gaccagcgcg cccaggcccg ctgggcgcgg cgcgggctgc gggtctcgtc
     3121 gacctggcag ggcctgaccg ccctcgacgg gctgcttgac cccgagcagg gcgagaccct
     3181 cctggcggcg ctgcagcccc tggcgcg
//
LOCUS       DASPYY010000005         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2528431, whole genome shotgun sequence.
ACCESSION   DASPYY010000005 DASPYY010000000
VERSION     DASPYY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528431"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VEP73_00050"
     CDS             complement(<1..379)
                     /locus_tag="VEP73_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT24862.1"
                     /translation="MGDRVLITGATGFVGGLLLTRLVREGRDVRVLVRKPADRERLPV
                     AGVELALGALGDDEALVRAADGCGVVYHVAGMNRLCLPDPSPLYRVNVEGTRRVLQAA
                     HKAGVRRVVYTSSAATLGGDGTRP"
     gene            complement(401..955)
                     /gene="pgsA"
                     /locus_tag="VEP73_00055"
     CDS             complement(401..955)
                     /gene="pgsA"
                     /locus_tag="VEP73_00055"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYT24863.1"
                     /translation="MGTLTLSTRITLLRIVLVPVLLALLVAEGRLANAEVLAALVFLV
                     ASFTDFIDGYLARRWQQVTTLGNFLDTTADKLLVMGALLGLLAIDRVDVWVAFVVIAR
                     EVVVVGLRAVAAAASVVISASIWGKLKFNAQVVGITLAILRPHVRIGALWLDEWAMLA
                     VAVVSAASALDYFSRFGDLVRAGR"
     gene            complement(1053..1337)
                     /locus_tag="VEP73_00060"
     CDS             complement(1053..1337)
                     /locus_tag="VEP73_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HYT24864.1"
                     /translation="MAERRAVVGSKVGLHARPAALFVRAAARQPVKVTIAKGGRDPVD
                     AASILAVLALDVRGGEEVVLAAGGDGAEQALDELVAVVEADHDAPAGGSG"
BASE COUNT          228 a          603 c          558 g          183 t
ORIGIN      
        1 ccggccgcgt cccgtcgccg cccagggtgg ccgctgagct ggtgtagacg acccggcgga
       61 cgccggcctt gtgggcggcc tggagcaccc tgcgggtgcc ctcgacgttg acccggtaga
      121 gcggggacgg gtcgggcagg cagagccggt tcatgccggc cacgtggtag accacgccgc
      181 agccgtcggc cgcccgcacg agcgcctcgt cgtcgccgag ggcgccgagg gccagctcga
      241 cgccggcgac cggcagccgc tcgcggtcgg cgggcttgcg gacgaggacc cgcacgtccc
      301 tgccctcgcg taccaggcgg gtgagcagca ggccgccgac gaacccggtc gccccggtga
      361 tcagcacacg gtcacccatc gacgccctcc cggcccatcg tcatcggccc gcccgcacga
      421 ggtcgccgaa gcgggagaag tagtcgaggg ccgaggcggc gctgacgacc gccacggcca
      481 gcatggccca ctcgtcgagc cagagcgccc cgatgcgcac gtgcgggcgc aggatggcga
      541 gcgtgatgcc gaccacctgg gcgttgaact tgagcttgcc ccagatcgac gcggagatca
      601 cgaccgaggc ggccgccgcg acggcccgca ggccgaccac gacgacctcg cgcgcgatca
      661 ccacgaaggc gacccagacg tcgacccggt cgatggcgag caggccgagc agggcgccca
      721 tcacgagcag tttgtcggcg gtcgtgtcga ggaagttgcc gagggtcgtg acctgctgcc
      781 accgccgggc caggtagccg tcgatgaagt cggtgaacga ggcgacgagg aagaccaggg
      841 cggcgagcac ctccgcgttc gccaggcgac cctcggcaac gagcaacgcg agcagcacgg
      901 gcaccagcac gatccgcaac agggtgatcc gcgtggacag cgtcagcgtc cccatcgaac
      961 ctcccgtcgt cgctccgatc ggccgtacct cgcatcctac gactggaagg ggcgattccc
     1021 ccagggaaac ctcggaaccg gccgggcgcc gctcagccac tgccgccggc cggcgcgtcg
     1081 tggtcggcct ccaccacggc gaccagctcg tcgagggcct gctcggcgcc gtcgccgccg
     1141 gcggccagga cgacctcctc gccgccgcgg acgtcgagcg cgaggacggc gaggatgctg
     1201 gccgcgtcga ccgggtccct gccgcctttg gcgatggtca ccttcaccgg ctgcctcgcg
     1261 gccgccctca cgaacagcgc ggccgggcgc gcgtggagcc ccacctttga accgacgacg
     1321 gcgcggcgct ctgccacggg tgcctcctcc tcttgacctg accggcctgc ccatcatcgt
     1381 tgccggcgcg gcctcgtgca cgcctgaccc tcacggcccc cgcgccggct ggccgtagga
     1441 gccgaaggcg tcggccaccc ggtccatctc ctcgtcgggc aggatcgccg gcgtgccgag
     1501 cgcgcgggcc cgccagcaca gctcggcggt gtactcgacc gcctcggcga cctcgagcgc
     1561 cgcgtcgagc cc
//
LOCUS       DASPYY010000006         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1685802, whole genome shotgun sequence.
ACCESSION   DASPYY010000006 DASPYY010000000
VERSION     DASPYY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685802"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2353)
                     /locus_tag="VEP73_00065"
     CDS             complement(71..2353)
                     /locus_tag="VEP73_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT24865.1"
                     /translation="MRSNLPGGPAGRVEDPPLVTGAARYTDDLAVPGALHAAFVRAVV
                     AHALVTAVATGEATARPGVVGVFTAADLGLPPLEPAGAPAAFARPVLAGERVRFLGEP
                     VAVVVADTRARALDAAELVGVDYEPLGVVTDPERALDPGAPVLFGGHGSNLVVEAPWR
                     GPPVELERADVVVRARFVNQRVAAVPMEPGAVVAVPDPATGGLTLWLPCQAPFFIRAA
                     VAAALGLDEGLVRVIAPAVGGGFGARIPAACEQVAVAALALRLGRPLRHVESRSESML
                     VSGHGRAQVQDVELGATADGTITDLRVRVLADGGAYPGDATIMPRMTRQMASGVYRIP
                     RVEFGYALVVTNTTPVGAYRGAGRPEAAALLERAVDLLAGELRLDPAELRRRNLIPAG
                     AFPYRTVSGATYDSGDYAGALRRVLEAAGYAGLRAEQAARRRRRDRLQLGVGLSTYVE
                     VTGFGPELGSCAVGPDGRVTVVTGTSPHGQGHETAWAQIVAGTLGLPVAAVHVVHSDT
                     GRVPRGNGTMGSRSLQVGGSAVLRAGERVLAKARELAAHLLEAPPEDVVVVEGEGLGV
                     AGAPGTALSWARLAEAAADPARLPPGMEPGLAAEVDFEQEGSTYPFGAHLAVVEVDVE
                     TGRARLVRHVAVDDCGRMLNPMLVDGQVHGGVAQGVAQALFEEVRYDEEGNCTTGSLA
                     SYAMPSAAELPGFETLHTETPTPLNPLGAKGIGESGAIGSTPAVQNAVADALAHLGVT
                     HVDMPLTPERVWRAIRAATA"
BASE COUNT          302 a          963 c          983 g          254 t
ORIGIN      
        1 cgacgccctc gagcattacg cggcgcaata ccccgacccg cgagcaccgc gcacggcctg
       61 aaattacctg tcaggccgtc gcggcccgga tcgcgcgcca gacccgctcc ggcgtgagcg
      121 gcatgtcgac gtgggtcacg ccgaggtggg cgagggcgtc ggccacggcg ttctgcaccg
      181 cgggggtcga cccgatcgcg ccggactcgc cgatgccctt ggcgccgagc gggttcaggg
      241 gggtcggggt ctcggtgtgg agcgtctcga agccgggcag ttcggccgcg ctcggcatgg
      301 cgtaggaggc gaggctgccg gtggtgcagt tgccctcctc gtcgtagcgg acctcctcga
      361 acagggcctg ggccaccccc tgcgcgacgc ccccgtggac ctggccgtcg accagcatcg
      421 ggttgagcat gcggccgcag tcgtcgaccg cgacgtggcg caccagccgc gcccggccgg
      481 tctcgacgtc gacctcgacg acggccaggt gggcgccgaa cgggtaggtg ctgccctcct
      541 gctcgaagtc cacctcggcg gccagccccg gctccatgcc gggggggagc cgggccgggt
      601 cggccgccgc ctcggcgagc cgggcccagg acagcgccgt cccgggcgcg cccgccacgc
      661 cgagcccctc accctcgacc accacgacgt cctccggcgg cgcctcgagc aggtgggcgg
      721 ccagctcgcg cgccttggcg aggacccgct cgccggcccg cagcacggcg ctgccgccga
      781 cctgcaggga gcgtgagccc atggtcccgt tgcccctcgg gacccggccg gtgtcggagt
      841 ggacgacgtg cacggcggcg accggcaggc cgagggtgcc cgcgacgatc tgggcccagg
      901 cggtctcgtg cccctgcccg tgcggggacg tcccggtgac gaccgtgacc cggccgtccg
      961 ggcccacggc gcagctgccg agctccggcc cgaagccggt cacctccacg taggtcgaca
     1021 ggccgacgcc gagctggagg cggtcccggc gccggcgcct ggcggcctgc tccgcgcgca
     1081 gcccggcgta gccggccgcc tcgaggacgc ggcgcagcgc gcccgcgtag tcccccgagt
     1141 cgtaggtggc gccggatacc gtccggtacg ggaaggcccc ggccgggatc aggttgcgcc
     1201 gccgcagctc cgccgggtcg agccggagct cgccggcgag caggtcgacg gcccgctcga
     1261 gcagcgcggc cgcctcgggc cggccggcgc cccggtacgc cccgaccggg gtcgtgttgg
     1321 tcaccaccaa ggcatagccg aactcgaccc gcgggatgcg gtagacgccg gaggccatct
     1381 gacgggtcat cctcggcatg atggtggcgt cgccggggta ggcgccgccg tcggcgagca
     1441 cccggacccg gaggtcggtg atcgtcccgt ccgcggtcgc gccgagctcg acgtcctgga
     1501 cctgggcgcg gccgtggccc gagaccagca tgctctcgga acggctctcc acgtgacgca
     1561 gcggccgccc gagccggagc gccagcgccg ccacggccac ctgctcgcaa gcggcgggga
     1621 tgcgcgcgcc gaagccaccc cccaccgccg gggcgatgac cctgaccagc ccctcgtcga
     1681 gcccgagcgc ggccgcgacg gccgcgcgga tgaagaaggg cgcctggcag ggcagccaga
     1741 gggtgagccc gccggtggcg gggtcgggca cggcgacgac ggcgcccggc tccatcggga
     1801 ccgcggcgac gcgctggttg acgaaccggg cgcgcaccac gacgtcggcc cgctccagct
     1861 cgaccggtgg tccgcgccag ggcgcctcga cgaccaggtt gctgccgtgg ccgccgaaca
     1921 gcacgggcgc gcccgggtcg agcgcccgct ccgggtcggt caccaccccg agcggctcgt
     1981 agtcgacgcc caccagctcg gcggcgtcga gcgcgcgggc ccgggtgtcg gcgaccacca
     2041 cggccaccgg ctcgccgagg aaccgcaccc gctccccggc caggaccggc cgggcgaagg
     2101 cggccggcgc gccggccggc tcgagcggcg gcaggcccag gtccgcggcg gtgaacaccc
     2161 cgaccacccc cggccgggcg gtcgcctcgc cggtggcgac cgcggtgacg agggcgtggg
     2221 cgacgaccgc gcggacgaag gcggcgtgga gcgcgccggg cacggccagg tcgtcggtgt
     2281 agcgggccgc gccggtgacg agcggggggt cctcgacgcg gcccgcgggg ccgccgggaa
     2341 ggttgctgcg catgccggta gcgtagggca accgccgcgc gggcgccccg gcggcggcgc
     2401 ggccgggccg gacgtccggg tcgcggcgca tactggaccg ccagggcgcg gtgcccgcca
     2461 ggcgccgacg gcgacggacg gaggccgagg atgagcgtcc ag
//
LOCUS       DASPYY010000007         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_141434, whole genome shotgun sequence.
ACCESSION   DASPYY010000007 DASPYY010000000
VERSION     DASPYY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141434"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VEP73_00070"
     CDS             complement(<1..390)
                     /locus_tag="VEP73_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT24866.1"
                     /translation="MAASLADVARRAEVSIATASRALNGSRHPVSAAVRERILAAADE
                     VGYRPSAVARALVTRRSRILGVVVGDIVDPYFAEITRGVEDRARKAGSLTMVCNADRR
                     TAAELAYLRVLRDYHAEGIIFAGGGYTA"
     gene            complement(491..1156)
                     /locus_tag="VEP73_00075"
     CDS             complement(491..1156)
                     /locus_tag="VEP73_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT24867.1"
                     /translation="MSSAGGEVLGTRTRVFLVDDHRFFLSGLRAELGAEFEIVGDGSD
                     VDASVEGIRRTGPDVVLLDVHLPGGGGRAVIEGVHRINPEVRFLALSVSDAAEDVIGV
                     IRAGARGYVTKTISPADLASAIRRVRDGDAVFSPRLAGFVLDAFRGRLSGPVDPELDQ
                     LTAREQEVLRHIARGYTYKEVARRLHLSVKTVETHVSSVLRKLQLSSRHELTRWATDR
                     RLV"
     gene            complement(1153..>2370)
                     /locus_tag="VEP73_00080"
     CDS             complement(1153..>2370)
                     /locus_tag="VEP73_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYT24868.1"
                     /translation="RSGTNRVAAGVAAGIGERLGIDPVLVRTALVVLALAGGAGVALY
                     LAGWALSREPGGDPAAGGRAAPADARPARREPAQRQLVALGCVVLGSLLLFREVGLWF
                     GDALVWPVALGAAGFAVIWTRGDSADRARWAAVGSRFPGNPFDDLFTGRVSLARVLLG
                     GLLVGSGMGVFLLANDALTFAAVRNVVLAVVVTAAGLAVILGPWMARLARQLADERRE
                     RIRSEERAEMAAHLHDSVLHTLALIQRADAPPEVATLARGQERELRAWLYGRTAPGGG
                     ERLSTAVEAVAARVEALHNVVVDAVVVGDGPMDERSWAIVHACQEAANNAARHSGAQR
                     VSVYVEAEPDAITAFVRDQGKGFDPEAVPGDRRGIAESIRGRMRRHGGTAVIASEPGE
                     GTEVHLRVPMEAA"
BASE COUNT          314 a          895 c          898 g          263 t
ORIGIN      
        1 ggcggtgtag ccgccgccgg cgaagatgat cccctcggcg tggtagtcgc gcaggacgcg
       61 caggtaggcg agctcggccg cggtcctccg gtcggcgttg cagaccatcg tgagggagcc
      121 ggccttgcgg gcacggtcct cgaccccgcg ggtgatctcg gcgaagtagg gatcgacgat
      181 gtcgccgacg accaccccga ggatgcggct gcgcctggtc acgagcgcgc gggccaccgc
      241 gctcggccgg tagccgacct cgtcggccgc ggccaggatg cgctcgcgga cagctgccga
      301 gacggggtgc ctggagccgt tcagcgcccg cgacgcggtc gcgatcgaga cctccgcccg
      361 tcgggcgacg tccgccagcg aggctgccac ggccatccct ccccgtcgga cgcgctgtgg
      421 agcaggccac cgcgatcgta cgagcccgtg gcaaaccttt gcaacgacgt gccgcccggg
      481 cgccaggcgc tcacaccagg cggcggtcgg tggcccagcg ggtcagctcg tggcggctcg
      541 agagctgcag cttgcggagc accgaggaca cgtgggtctc caccgtcttc accgagaggt
      601 gcagccggcg ggcgacctcc ttgtaggtgt agcccctggc gatgtgcctg aggacctcct
      661 gctccctcgc ggtgagctgg tccagctcgg ggtcgacggg gccgctgagc cggccccgga
      721 aggcgtcgag cacgaagccg gcgaggcggg gcgagaagac ggcgtcgccg tcgcggaccc
      781 ggcggatggc gctggccagg tcggcggggg agatcgtctt ggtcacgtag ccgcgggcgc
      841 cggcccggat cacgccgatg acgtcctcgg ccgcgtcgga caccgacagc gcgaggaacc
      901 tgacctccgg gttgatgcgg tgcacgccct cgatcaccgc ccgcccgccc ccgccgggca
      961 ggtggacgtc gagcaggacg acgtccgggc cggtcctccg gatgccctcg accgaggcgt
     1021 cgacgtcgct cccgtcgccg acgatctcga actcggcacc cagctcggcg cgcaggcccg
     1081 acaggaagaa gcggtggtcg tcgaccagga acacccgggt gcgcgtgccc agcacctccc
     1141 cgccggcgga gctcacgcgg cctccatggg gacgcgcagg tgcacctcgg tgccctcgcc
     1201 gggctcgctc gcgatgacgg ccgtgccgcc gtgccggcgc atgcggccgc ggatcgactc
     1261 ggcgatgccg cgccggtcgc cgggcaccgc ctcggggtcg aagcccttgc cttggtcgcg
     1321 gacgaaggcg gtgatcgcgt ccggctccgc ctcgacgtag accgacacgc gctgcgcgcc
     1381 cgagtgcctg gccgcgttgt tggccgcctc ctggcaggcg tgcacgatcg cccacgaccg
     1441 ctcgtccatc gggccgtcgc ccacgaccac ggcgtcgacc acgacgttgt ggagcgcctc
     1501 gacccgggcc gcgaccgcct ccaccgccgt gctcaggcgc tccccgccgc ccggggcggt
     1561 ccgcccgtag agccaggccc gcagctcgcg ctcctggccg cgggccaggg tggccacctc
     1621 gggcggcgcg tcggcgcgct ggatcagggc cagcgtgtgg agcaccgagt cgtgcaggtg
     1681 ggcggccatc tccgcgcgct cctccgagcg gatgcgctcg cgccgctcgt cggcgagctg
     1741 ccgggccagc cgggccatcc agggaccgag gatgaccgcg aggccggccg cggtgaccac
     1801 gacggcgagc acgacgttgc ggaccgccgc gaacgtcagg gcgtcgttgg cgagcaggaa
     1861 cacgcccatg ccgctgccca cgagcaggcc gccgaggagc acccgggcga gcgacacccg
     1921 ccccgtgaac aggtcgtcga acgggttgcc cgggaaccgc gagccgacag cggcccaccg
     1981 ggcccggtcg gccgagtcgc cgcgggtcca gatgacggcg aacccggcgg cgccgagggc
     2041 gaccggccac accagggcgt caccgaacca gagcccgacc tcgcggaaca ggagcaggct
     2101 gccgagcacg acgcacccca gcgcgacgag ctgccgctgg gccggctcgc ggcgggccgg
     2161 gcgggcatcg gcgggcgcgg cgcggccgcc ggccgcaggg tcgccgccgg gctcacggct
     2221 gagcgcccac ccggccaggt agagggcgac tcccgcgccg ccggcgaggg cgagcacgac
     2281 cagcgcggtc cgcacgagca cggggtcgat gcccaggcgc tcgccgatac cggccgccac
     2341 gcccgcggca acgcggttgg tgccgctgcg
//
LOCUS       DASPYY010000008         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1967595, whole genome shotgun sequence.
ACCESSION   DASPYY010000008 DASPYY010000000
VERSION     DASPYY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967595"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VEP73_00085"
     CDS             complement(<1..563)
                     /locus_tag="VEP73_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT24869.1"
                     /translation="MNRLVRAELAKLRTTRTTPVLLATMAAFAALLTAAILTLAGHQG
                     NPPLDANALRTFVGAPSRLLSGVVLLVGILAMAGELRHTTITPTFLVAPDRARVVVAK
                     LAAVALAGLFFAVVAEAVALAVAVPALAAKGVAVHVTGAVGRELAGVLVVAALSGMLG
                     VAVAALVGNQLAAVVGAVLWVFVAESVL"
     gene            complement(560..1486)
                     /locus_tag="VEP73_00090"
     CDS             complement(560..1486)
                     /locus_tag="VEP73_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT24870.1"
                     /translation="MPAITVEGLTKRFGAVTAVDDLSFEVGEGTVTGFLGPNGAGKTT
                     TLRTLLGLVSPTSGTATFGGRPYRRLPEPSREVGAVLEASGFHPGRSARDHLRVLAIA
                     AGVPAGRTDEVLALVGLAEAAGRRVGGFSLGMRQRLALAAALLGDPRVLVLDEPANGL
                     DPEGVHWLRGLLRAFAREGRAVLVSSHLLAEMAQTADHVVIVARGRLVAQSSLADLTA
                     RAGQRVHVRTPQAEALHAALAARGATVERGGPDELLVGGVDTEAVGRAVAEAGVVVYE
                     MRLERPDLEAAFLELTADQLTREELTREEVSS"
BASE COUNT          217 a          607 c          693 g          179 t
ORIGIN      
        1 agcacgcttt cggcgacgaa gacccacagc accgcgccga cgacggcggc gagctggttg
       61 ccgaccaggg cggcgacggc gacgccgagc atccccgaca gcgccgcgac gaccaggaca
      121 ccggccagct cgcggccgac cgcgccggtg acgtggacgg cgacgccctt ggcggccagc
      181 gcgggcacgg ccaccgcgag cgccaccgcc tcggcgacca ccgcgaagaa gagcccggcc
      241 agggcgacgg cggcgagctt ggccacgacg acgcgggcgc ggtcgggggc gacgaggaag
      301 gtcggggtga tggtggtgtg cctcagctcc ccggccatgg ccaggatgcc gaccagcagg
      361 acgacgcctg acagcaggcg gctgggcgcg ccgacgaagg tgcggagggc gttcgcgtcg
      421 aggggcggat tgccctggtg gccggcgagc gtgaggatgg ccgccgtcag cagggccgcg
      481 aacgccgcca tggtggcgag caggacgggg gtggtgcggg tggtgcggag cttggccagc
      541 tcggcacgga cgagcctgtt cacgacgaga cctcttccct ggtgagttct tccctggtga
      601 gctggtcggc ggtgagctcg aggaacgccg cttcgaggtc ggggcgctcg agccgcatct
      661 cgtagacgac cacgccggcc tcggccacgg cccggccgac ggcctcggtg tcgacgccgc
      721 cgaccagcag ctcgtccggg ccgcctcgct cgacggtcgc gccacgggcg gcgagcgcgg
      781 cgtggagcgc ctcggcctgc ggggtgcgga cgtggacgcg ctggcccgcc cgggcggtga
      841 ggtccgcgag gctggactgg gcgaccagcc ggccgcgggc gacgatcacg acgtggtcgg
      901 cggtctgggc catctcggcc agcaggtggc tcgagacgag caccgcgcgg ccctcgcggg
      961 cgaaggcccg caggagcccg cggagccagt gcacgccctc ggggtccagg ccgttggccg
     1021 gctcgtcgag gaccagcacg cgagggtcgc cgagcagcgc ggcggccagg gcgagacgct
     1081 ggcgcatgcc cagcgagaag ccgccgaccc gccgcccggc cgcctcggcc aggccgacga
     1141 gggccaggac ctcgtcggtc cgtccggccg ggacgccggc cgcgatcgcg agcacgcgca
     1201 ggtggtcccg ggccgagcgg ccggggtgga agccggacgc ctccagcacc gcgccgacct
     1261 cgcggctcgg ctcggggagc ctccggtagg ggcggccgcc gaacgtggcc gtcccgctgg
     1321 tcgggctgac cagcccgagc agggtccgca gggtggtggt cttgccggcg ccgttcgggc
     1381 cgaggaagcc cgtgaccgtg ccctcgccga cctcgaagct gaggtcgtcg acggcggtga
     1441 cggcgccgaa gcgcttggtg agcccttcga cggtgatggc tggcatgatc gctccttcgc
     1501 ctggagcccg cccctggtcg agccggcggg acgctcctga cggtagggac cggagcgccg
     1561 cgcgtcgtcg gccccggagc gacacttcgg ggccgccctc gggacgacgg cgcgtcgccc
     1621 cctgggcgta cgcgacgctc ggcccctggg cgtacgccgg ggcgagggcc ggcgcggccc
     1681 gcggctcagg ccgagc
//
LOCUS       DASPYY010000009         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1372, whole genome shotgun sequence.
ACCESSION   DASPYY010000009 DASPYY010000000
VERSION     DASPYY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VEP73_00095"
     CDS             <1..1668
                     /locus_tag="VEP73_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYT24871.1"
                     /translation="AVRAARTELARADAATARTPVRVARRLPVLSSPVADLGHLLAAG
                     HLLVRTADRAVTVEAQVTGGKGTLFRDGRIDLATAAGAISAASGILDDLGRARDELRR
                     VRGGRLAPGAAGARDSTLREVDKLDGQVRPLVALLGALPPAVGAEGPRTYLVVVMNSA
                     ELKAMGGAPLAIALVRLEQGRVSVLRRGEVSEQRLNLGVRWPYVPGDPWHPPGSTSRF
                     TSSGLSPDFPTSGEEVLRAYQAITGIRANGLIALDPSALASLLRVTGPVSSPGYGTVK
                     AGSVVRLLLADSYQRFPSKDLRHAVNAALMDAVLRRVLAGGKLLGQVRALGEAAKGRH
                     LQLYFRDAGLQRTALAHRLAGALSAAPDDYLAVYTQNGNASKVDYYQRRSVEQVVRLS
                     PDGSARVTRRIRVANATPPAGPADRSKTGYLTGWARPVIIAYVPGRATGVSARVDGRL
                     AHPASFRELGRQAVRFLTSMPPGSTSTVTVEYRLPGAAVRDGAGMTYALAADTQPIVD
                     PASLRVVVVPPPGFSAAPQAGWTAEKGTLVATRQFTSDQSLTLRLEG"
     gene            complement(1628..>2010)
                     /locus_tag="VEP73_00100"
     CDS             complement(1628..>2010)
                     /locus_tag="VEP73_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT24872.1"
                     /translation="ELDVDPVRIGEVLANLLSNAVRHTPRGGSVVVAVEPSAAGVCVA
                     VSDTGSGIGAGDLPYVFDRFVKSADSGGAGLGLAIAKRLVEAHGGEISAESEPGGGTT
                     MRLVLPREGGPVSPRGGGSATGRS"
BASE COUNT          243 a          808 c          718 g          241 t
ORIGIN      
        1 gccgtgcggg ccgccaggac cgagctggcc agggcggacg ccgccacggc gcgcaccccg
       61 gtccgcgtcg cgcggcgcct gcccgtcctc tcctcccccg tggccgacct gggccacctg
      121 ctcgccgccg ggcacctgct cgtgcgcacg gccgatcggg ccgtcaccgt cgaggcccag
      181 gtgaccggcg ggaaggggac gctgttccgg gacggccgca tcgacctcgc caccgcggcg
      241 ggcgccatct cggcggcctc gggaatcctc gacgacctcg gccgtgcccg cgacgagctg
      301 cgccgcgtcc gcgggggccg gctcgccccc ggcgcggcgg gggcgcgcga ctcgaccctc
      361 cgggaggtcg acaagctcga cgggcaggtc cgcccgctcg tcgcgctgct cggagcgctg
      421 ccgcccgcgg tcggggccga gggcccgcgc acctacctgg tggtggtgat gaactccgcc
      481 gagctgaagg cgatgggcgg agcgccgctc gcgatcgccc tcgtccggct cgagcagggc
      541 agggtctcgg tcctgcgccg cggcgaggtg agcgagcagc ggctcaacct gggcgtccgc
      601 tggccctacg tgccaggcga cccgtggcac ccgcccgggt cgacctccag gttcacgagc
      661 tccggcctct cgcccgactt ccccacctcg ggcgaggagg tgctccgcgc ctaccaggcg
      721 atcaccggca tccgcgcgaa cgggctgatc gccctcgacc cgagcgccct ggcctcgctg
      781 ctgcgcgtga ccgggccggt gtcgagcccc gggtacggca ccgtgaaggc gggcagcgtc
      841 gtccgcctcc tgctcgccga cagctaccag cggttcccgt ccaaggacct gcgtcacgcg
      901 gtcaacgcgg cgctgatgga cgcggtgctc cgccgcgtcc tggccggcgg caagctcctc
      961 ggccaggtgc gcgccctcgg cgaggcggcc aagggcaggc acctgcagct ctacttccgg
     1021 gacgccgggc tgcagcgcac cgccctggcc caccggctcg ccggtgccct gtccgcggca
     1081 cccgacgact acctcgccgt ctacacgcag aacggcaacg ccagcaaggt ggactactac
     1141 cagcgccgct cggtcgagca ggtggtgcgg ctctcgccag acgggtcggc ccgggtgacc
     1201 cgcaggatcc gggtggccaa cgccacgccg cccgccggac cggcggaccg gtcgaagacg
     1261 ggctacctga cgggctgggc acgcccggtg atcatcgcct acgtgcctgg cagggccacc
     1321 ggcgtgtccg cgcgcgtcga cggcaggctc gcccacccgg cgtcgttccg ggagctcgga
     1381 cgccaggccg tccgcttcct gacgtccatg ccgcccggct cgacgagcac ggtcacggtc
     1441 gagtaccggc tgcccggggc ggccgtcagg gacggcgccg gcatgacgta cgcgctcgcg
     1501 gccgacaccc agccgatcgt cgaccccgcc tcgctgcggg tcgtggtggt gccgccgcca
     1561 gggttcagcg ccgcgccgca ggccggctgg acggcggaga agggcaccct cgtcgcgacc
     1621 cgccagttca cgagcgacca gtcgctgacc ctccgcctcg agggctgacc ggaccaccct
     1681 cccggggcag caccagccgc atcgtcgtgc cgccacccgg ctcgctctcg gcgctgatct
     1741 cgccgccgtg cgcttcgacg aggcgcttgg cgatggcgag ccccaggccc gcgccccccg
     1801 agtcggccga cttgacgaac cggtcgaaca cgtaggggag gtccccggcg ccgatgcccg
     1861 agccggtgtc cgagacggcg acgcagaccc ctgccgccga cggctccacc gcgaccacga
     1921 ccgagccgcc ccgcggcgtg tgcctgacgg cgttcgacag caggttcgcg agcacctccc
     1981 cgatccgcac cgggtcgacg tccagctcgc
//
LOCUS       DASPYY010000010         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2669985, whole genome shotgun sequence.
ACCESSION   DASPYY010000010 DASPYY010000000
VERSION     DASPYY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669985"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VEP73_00105"
     CDS             <1..1329
                     /locus_tag="VEP73_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYT24873.1"
                     /translation="IDAVFRQCGVLRAHGFSDLQDFAQGFSRAGRLPRGRRVAIVTNA
                     GGPAVMTTDALEGSGLEVAELAEVTRDRLELVLPEAASTANPVDVLGDAQADRYDEAL
                     DAVMRDPGVDATIVLLTPQVMTEPETTARHVVREAKQSDKPVIAVFMGGEDIAGGVHV
                     LDKGRVPVYDSPERAVRTLAMLAYYHDYLHFEREPAPVFEVDRAAVASVLDGARERGD
                     RTLTGLEAARLFGAYGFPVLEGRVVGGPDEAVAHAEKAGWPVALKIASADVLHKMDVG
                     AVRVDLRTETALRRAYGEILANVREHVPQARIDGMLVEPMAPRGREVILGMSRDPQFG
                     PVILFGLGGVYVEVLKDVTFRLAPMLPLSARRMLGEIRARALLDGVRGEPPSDKAAIV
                     DGLLRLSQLAVEQPGIAEVDVNPLIVTASGATGVDYRVILTEPGAAAAQR"
     gene            complement(1418..2233)
                     /locus_tag="VEP73_00110"
     CDS             complement(1418..2233)
                     /locus_tag="VEP73_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYT24874.1"
                     /translation="MASPARRAGMAGASWPPRAALVRPPGASFGRALSEHPDRDTIDP
                     DRAAAQHAGYRDALRRAGLDVVVLPVAEELPDACFVDDCAVVAGGDALLTRPGAPSRA
                     AEPELLAADLGRLAGRVERMAPPATLDGGDVLALGDSLVVGRSRRTNQAGIDRLAAFA
                     AGRGLRVAVAELPAGTLHLQSSVTALGARAVVGRPDVLDQPAFGPAEARVEVPPDEAP
                     ACNVVVAGGTIVMPAGCPRTAAALAALGFAVDELDLSEFAKADGGATCLSLLL"
     gene            complement(2226..>2582)
                     /locus_tag="VEP73_00115"
     CDS             complement(2226..>2582)
                     /locus_tag="VEP73_00115"
                     /inference="COORDINATES: protein motif:HMM:NF017806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HYT24875.1"
                     /translation="RRVTILAEARKRVQRLGATHPLPVEVVRFAWPATRRRLLALVPE
                     AVLRRGPSGDPFVTDEAHHLLDCLMPDVDPHELAAAVKVTLGVVDHGLFLDQADEVLL
                     GRPDGTVEVLTRRRDG"
BASE COUNT          333 a          934 c         1027 g          288 t
ORIGIN      
        1 atcgacgccg tcttccggca gtgcggggtg ctgcgcgcgc acggcttctc ggacctgcag
       61 gacttcgccc agggcttcag ccgggccggg cggctgccac gggggcgccg cgtggcgatc
      121 gtcacgaacg ccggcggtcc cgccgtgatg accaccgacg cgctcgaggg ctcgggactc
      181 gaggtcgccg agctggccga ggtgacgagg gaccgcctgg agctggtgct gcccgaggcg
      241 gcgtcgaccg ccaacccggt cgacgtcctc ggcgacgccc aggccgaccg ctacgacgag
      301 gcactcgacg cggtcatgcg cgatcccggg gtggacgcga ccatcgtcct gctcaccccc
      361 caggtgatga ccgagccgga gaccaccgcc cgccacgtcg tccgggaggc caagcagagc
      421 gacaagccgg tcatcgccgt gttcatgggc ggggaggaca tcgccggcgg ggtgcacgtg
      481 ctggacaagg gccgggtgcc ggtctacgac tcgcccgagc gggccgtgcg gacgctcgcc
      541 atgctcgcct actaccacga ctacctgcac ttcgagcgcg agccggcgcc ggtgttcgag
      601 gtcgaccggg ccgcggtcgc aagcgtgctc gacggcgcgc gcgagcgcgg ggaccggacc
      661 ctgaccggcc tggaggcggc ccggctgttc ggcgcctacg gcttcccggt cctcgaaggc
      721 cgggtcgtgg ggggcccgga cgaggcggtc gcccacgccg agaaggctgg ctggccggtg
      781 gcgctcaaga tcgcctcggc cgacgtgctc cacaagatgg acgtcggcgc ggtccgcgtc
      841 gacctgcgca cggagacggc gctccgccgg gcgtatgggg agatcctcgc caacgtgcgc
      901 gagcacgtcc cgcaggcgcg catcgacggg atgctggtcg agccgatggc gccgcgcggc
      961 cgggaggtca tcctgggcat gagccgcgac ccgcagttcg gccccgtgat cctgttcggc
     1021 ctgggcggcg tgtacgtgga ggtgctgaag gacgtcacct tccggctcgc gcccatgctg
     1081 ccgctgtcgg cccggcgcat gctcggcgag atccgcgcga gggcgctgct cgacggcgtc
     1141 aggggcgagc cgccgtccga caaggcggcg atcgtcgacg gcctgctgcg cctctcccag
     1201 ctcgccgtcg agcagcccgg gatcgccgag gtcgacgtca acccgctgat cgtcacggcc
     1261 agcggcgcca cgggcgtcga ctaccgggtc atcctgaccg agcccggcgc ggccgccgcc
     1321 cagcggtagg agcctggcat cccccggcgc aggtcacagc aggtcacaag accctgcgcc
     1381 cttgcgcagg tcacaagacc ctgcgccctt gcgcaggtca taagaggagc gacaggcagg
     1441 tcgcgccgcc gtcggccttg gcgaactccg acaggtcgag ctcgtcgacc gcgaagccga
     1501 gggcggcgag cgcggcggcg gtgcgcgggc agcccgccgg catcacgatc gtgccgcccg
     1561 ccacgaccac gttgcacgcg ggcgcctcgt cgggcgggac ctcgaccctg gcctcggccg
     1621 gaccgaacgc gggctggtcg agcacgtcgg ggcggccgac gaccgcgcgg gcgccgagcg
     1681 cggtcaccga cgactgcagg tgcagcgtgc cggccgggag ctcggcgacg gcgacgcgga
     1741 gcccgcggcc ggcggcgaag gcggcaagcc ggtcgatccc ggcctggttg gtccgccggg
     1801 agcggccgac gacgagcgag tcgccgagcg cgaggacgtc cccgccgtcc agggtggcgg
     1861 gcggcgccat ccgctcgacc cggccggcca gcctgccgag gtcggcggcg agcagctccg
     1921 gctcggccgc cctcgacggc gcgccaggcc gggtgagcag cgcgtccccg cccgccacga
     1981 ccgcgcagtc gtccacgaag caggcgtcgg gcagctcctc ggcgacgggg agcacgacca
     2041 cgtcgaggcc ggcgcggcgc agggcgtcgc ggtacccggc gtgctgggcc gcggcccggt
     2101 ccgggtcgat cgtgtcgcgg tccgggtgct cggacagcgc gcggccgaac gaggcgcccg
     2161 gcggccgcac gagcgcggcg cgcggcggcc aggaggcgcc agccatcccg gcgcggcgcg
     2221 cggggctagc catcccggcg ccgcgtgagg acctcgacgg tgccgtcggg gcggccgagg
     2281 aggacctcgt cggcctggtc gaggaagagg ccgtggtcga cgaccccgag ggtgaccttg
     2341 acggcggcgg ccagctcgtg cgggtcgacg tcgggcatca ggcagtcgag caggtggtgg
     2401 gcctcgtcgg tgacgaaggg gtcgccggac gggccccggc ggagcacggc ctccgggacg
     2461 agggcgagca ggcggcgccg tgtcgccggc caggcgaagc ggaccacctc gaccgggagc
     2521 gggtgcgtgg cgccgaggcg ctggacgcgc ttgcgggcct cggcgaggat cgtgacccgg
     2581 cg
//
LOCUS       DASPYY010000011         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2248642, whole genome shotgun sequence.
ACCESSION   DASPYY010000011 DASPYY010000000
VERSION     DASPYY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248642"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VEP73_00120"
     CDS             <1..905
                     /locus_tag="VEP73_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24876.1"
                     /translation="LLFLPLAGDGLMTALRLAGSSLFTLGIASSSREGATVVTFVAAA
                     TGLVVVALEIGYLPTIYGAYNRRETLVNMLESRAGEPAWGVELLAREQLIGGLDTLAP
                     FFSDWERWAADLTESHVNYPWLLSFRSPYPLRSWVVSLLAVLDAAALYLALVPSRAPS
                     AARHCLRTGFLGLRALAGVYKIELPADPRPDAPIALTYEEFEAGLDRLQAVGFPMERS
                     REEAWRHFRGWRVNYETAAYLLADNLVAVPAPWSGGRRSLRLRSDQPLEPVRPRHRSP
                     EDPEGRVGLLPPRSEPSERDGQAP"
     gene            993..1751
                     /locus_tag="VEP73_00125"
     CDS             993..1751
                     /locus_tag="VEP73_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT24877.1"
                     /translation="MMNVLNARGLVKQYRRGRAVDHVDLVVRSGERVALLGPNGAGKT
                     TTLLMCLGVVEPDAGTVDVCGHRLPKGRSAAMARVGFTAGYLPLPERLRVREYLNLYG
                     RLYGLADPDGQAAKGLERFGVGHLGVAMGTELSSGQKTLVGIVKATMHDPQLLVLDEP
                     TASLDPDVALRVRTGLAALCAEEGTALLITSHDMLEVERIAERVVFLASGRIVADGPP
                     AEVAAGFGQGDLEEVFLHLAREREAREHQEGARP"
     gene            1748..2575
                     /locus_tag="VEP73_00130"
     CDS             1748..2575
                     /locus_tag="VEP73_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT24878.1"
                     /translation="MSAVRVSPRQAALLRVRSVARRHAYTLWRSPHRLFDVTIWPLVD
                     TVLFGSIGIFFARQGGPGTSAQATAGYLLAGIVLWHVVYQAQIAVATGFLEETWSRNL
                     LSLMVTPLREAEYVAGVALFGLVKLVMGVAVVTVGAFAFYAFDVTDLGLGLLPVAVVL
                     LVAGWAISLFVIGLVLRFGNGAEAFAWGILFVVMPLSGVFYPLSALPAFLRPVSMLLP
                     TTHAFAAGRTLVAGRGMDWDELGLAALTTVLLTAGALALLARMLRLFRRRGYITRYM"
     gene            complement(2602..>2941)
                     /locus_tag="VEP73_00135"
     CDS             complement(2602..>2941)
                     /locus_tag="VEP73_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYT24879.1"
                     /translation="DRLGSGQPPGHLGVAVGTYHVWWDPLVHAQLARAAGRILCFQVS
                     DWLLPVGDPLHGRGLPGDGLIEVRRLRTEADAAGYDGPIEVEIFNHDLWAEEGRQVLE
                     RMRASYLEHV"
BASE COUNT          328 a         1114 c         1065 g          434 t
ORIGIN      
        1 cgctgctgtt cctgccgctg gcaggcgacg gcctcatgac cgcgctccgc ctggccggct
       61 cgtcgctgtt caccctcggc atcgcctcct cctccaggga gggggccacc gtggtcacgt
      121 tcgtcgccgc ggccaccggc ctcgtcgttg tcgccctcga gatcggctac ctgcccacca
      181 tctacggcgc ttacaaccgc cgcgagaccc tggtcaacat gctcgagagc cgcgcggggg
      241 agcccgcctg gggggtcgag ctgctcgccc gcgagcagct catcgggggc ctcgacacgc
      301 tcgccccgtt cttcagcgac tgggagcggt gggccgccga cctcaccgag agccacgtga
      361 actacccgtg gctgttgtcc ttccgctcgc cgtatccgct gcgctcgtgg gtggtcagcc
      421 tgctcgcggt gctggacgcc gccgccctgt acctcgcgct cgtcccgagc cgggcgccgt
      481 cggcggcccg gcactgcctg cgcaccggct tcctcgggct gcgggccctg gccggcgtct
      541 acaagatcga gctgcccgcc gacccccggc ccgacgcgcc gatcgccctc acctacgagg
      601 agttcgaggc cgggctcgac cgcctgcagg ccgtcgggtt cccgatggag cggtcccggg
      661 aggaggcctg gcgccacttc cgcggctggc gggtgaacta cgagacagcc gcctacctgc
      721 tcgccgacaa cctggtggcg gtgcccgcgc cctggtcggg cgggcgccgc agcctgcgcc
      781 tgcgctccga ccagccgctg gagccggtcc gcccccgcca ccgcagcccc gaggaccccg
      841 agggccgcgt cggcctgctc ccgccgcggt ccgagccgtc cgagagggac ggccaggcgc
      901 cctgacgccc cgcgggccgg cggcgctcgc ggccgggctg acccggtgga aacccgctgg
      961 cgactgtcgg atgcggctgt gacactgggc gcatgatgaa cgtcctgaac gcccggggcc
     1021 tcgtgaagca gtaccgccgc ggccgggccg tcgaccacgt cgatctcgtc gtccggagcg
     1081 gcgagcgcgt cgcgctgctc gggccgaacg gcgccggcaa gaccaccacg ctgctcatgt
     1141 gcctcggggt ggtcgagccg gacgccggca cggtggacgt ctgcggccac cggctgccga
     1201 aggggcgcag cgcggccatg gcccgggtcg gcttcacggc gggctacctg ccgttgcccg
     1261 agcgcctccg cgtgcgcgag tacctcaacc tctacgggcg gctctacggg ctcgccgacc
     1321 cggacgggca ggccgccaag gggctcgagc gcttcggcgt cgggcacctc ggggtcgcca
     1381 tgggcaccga gctctcgtcg ggccagaaga ccctcgtcgg gatcgtcaag gcgaccatgc
     1441 acgacccgca gctgctcgtg ctcgacgagc cgaccgcctc gctcgacccc gacgtggccc
     1501 tgcgggtgcg caccgggctc gccgccctct gcgccgagga ggggacggcg ctgctcatca
     1561 ccagccacga catgctcgag gtggagcgga tcgccgagcg ggtcgtgttc ctggccagcg
     1621 gccgcatcgt ggccgacggc ccgcccgccg aggtggcggc cggcttcggg cagggggacc
     1681 tcgaggaggt cttcctgcac ctggccaggg agcgcgaggc gcgcgagcac caggaggggg
     1741 cgcggccatg agcgccgtcc gcgtgtcgcc gaggcaggcc gcgctcctgc gcgtccggtc
     1801 ggtcgcccgc aggcacgcct acacgctctg gcgcagcccc caccgcctgt tcgacgtcac
     1861 catctggccg ctcgtcgaca cggtgttgtt cggctcgatc gggatcttct tcgcccgcca
     1921 gggcggcccg ggcacgagcg cccaggccac ggccggctac ctgctcgccg gcatcgtcct
     1981 gtggcacgtc gtctaccagg cccagatcgc ggtcgccacc ggcttcctcg aggagacctg
     2041 gtcgcggaac ctgctcagcc tgatggtcac gcccctgcgg gaggcggagt acgtggccgg
     2101 cgtggccctc ttcgggctcg tgaagctcgt catgggcgtg gccgtcgtca cggtcggggc
     2161 gttcgcgttc tacgccttcg acgtgaccga cctgggcctc gggctgctgc cggtcgccgt
     2221 ggtcctgctc gtcgccggct gggccatctc gctgttcgtg atcggcctgg tcctgcggtt
     2281 cggcaacggc gccgaggcgt tcgcctgggg gatcctgttc gtggtgatgc cgctgtccgg
     2341 ggtcttctac cccttgagcg ccctgccggc gttcctgcgg ccggtgagca tgctgctgcc
     2401 cacgacccac gcgttcgcgg ccggccggac gctcgtcgcc gggcgcggca tggactggga
     2461 cgagctcggg ctcgccgcgc tgacgaccgt gctgctcacc gccggcgcgc tcgccctgct
     2521 cgcccggatg ctgcgcctgt tccgtcgccg cggctacatc acgcgctaca tgtagcggcc
     2581 ggccagggcg acggcggcgt gtcagacgtg ctcgaggtag ctcgcgcgca tccgctcgag
     2641 cacctgccgg ccctcctcgg cccagaggtc gtggttgaag atctccacct cgatcgggcc
     2701 gtcgtagccg gcggcgtccg cctcggtgcg gaggcggcgc acctcgatca gcccgtcgcc
     2761 cggcaggccg cgcccgtgga gggggtcgcc gaccggcagg agccagtcgc tcacctggaa
     2821 gcagaggatg cgtcctgccg cgcgggcgag ctgggcgtgg acgagcggat cccaccacac
     2881 gtggtaggtg ccgaccgcca ccccgaggtg gccgggaggt tggccggagc cgaggcggtc
     2941 g
//
LOCUS       DASPYY010000012         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_704171, whole genome shotgun sequence.
ACCESSION   DASPYY010000012 DASPYY010000000
VERSION     DASPYY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704171"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..823)
                     /locus_tag="VEP73_00140"
     CDS             complement(56..823)
                     /locus_tag="VEP73_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYT24880.1"
                     /translation="MVALHPPQVAVLAGAALAAGAVNAVAGGGSLVSFPALLAVGYPA
                     IDANVTNTVALCPGYFGGTLGYRRELSGQRARVRALGATSVVGALLGAFLLVISSPEL
                     FKLIVPFLIFLACGLLAVQPWLARLVQGRGRPRRAEHRPVALYGVQFLAAVYGAYFGA
                     GVGIMMLAILGIFLSDSLQRLNALKGLLSLVINVVAAAYFALFAHVAWLAAALMAPAA
                     LLGGQVGVLLARRLDDRVLRVLVIAFGVAVGVRLLVS"
     gene            1002..1241
                     /locus_tag="VEP73_00145"
     CDS             1002..1241
                     /locus_tag="VEP73_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24881.1"
                     /translation="MSETISPLEQATVTSVGQLVQVLRAFPPDTPVEAGLGYRDETDS
                     YRTVTVSAVTSDWLDGRPDVTTVIVLGETGSLRAP"
     gene            1286..1498
                     /locus_tag="VEP73_00150"
     CDS             1286..1498
                     /locus_tag="VEP73_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24882.1"
                     /translation="MSQTQAPPIEKGAQVKVRRPGSGESGVWKVIGPVASSGPDDPAY
                     DVTNLISGRQRVFRASRLIVLGAAKR"
     gene            complement(1540..>1959)
                     /locus_tag="VEP73_00155"
     CDS             complement(1540..>1959)
                     /locus_tag="VEP73_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta endotoxin C-terminal domain-containing
                     protein"
                     /protein_id="HYT24883.1"
                     /translation="TVAPTTAPPATVALRFSREAEAGGNTLGGGARIVTCQGCSGGAE
                     VSFVGNGGTLQFNGVTAPSLGRYQVAISYASGEQRSADLSVNGSAGIRLDFAGTGSFD
                     TVGVRTVTVTLRAGPNTLMLSGGHFTPGFDGIRVQPA"
BASE COUNT          306 a          684 c          763 g          206 t
ORIGIN      
        1 ccgttcggca cgccttgctc tgtcccgttc ggcacgccga ggcgtgcctc cgacgtcagg
       61 agacgagcag gcgcacgccg accgccacgc cgaaggcgat cacgaggacg cgcagcacgc
      121 ggtcgtcgag gcggcgggcg agcagcacgc ccacctggcc gccgagcagg gcggcggggg
      181 ccatgagcgc ggccgcgagc cacgccacgt gggcgaagag cgcgaagtag gcggcggcga
      241 cgacgttgat gaccagggag aggaggccct tcagggcgtt caggcgctgc aggctgtcgg
      301 agaggaagat gccgaggatc gcgagcatca tgatcccgac cccggccccg aagtaggcgc
      361 cgtagacggc ggcgaggaac tgcaccccgt acagggcgac gggacggtgc tccgcgcgcc
      421 gcggccgccc gcggccctgg acgagccggg cgagccatgg ctgcaccgcg agcaacccgc
      481 aggccaggaa gatcaggaac ggcacgatca gcttgaacag ctcgggcgag ctgatcacca
      541 gcaggaaggc gccgaggagg gcgcccacga cgctggtcgc gcccagggcg cggacccggg
      601 cacgctgccc cgagagctcc ctgcggtagc cgagggtgcc gccgaagtag ccggggcaga
      661 gggccacggt attggtgacg ttggcgtcga tggccgggta gcccacggcc agcagggcgg
      721 ggaacgagac gagcgagccg ccgccggcga ccgcgttgac ggccccggcg gcgagtgccg
      781 ccccggcgag cacggcgacc tgggggggat ggagggcgac cacggcgaca gcctacgtcg
      841 tccgcgccag cgggcggcgg gtggccgctg gcgccgtcca caggggcggc ccgggaatgg
      901 acggcgatgg ggccccggga gtagcattac cgaacacacg ttcgagcacg cgccgccggg
      961 cgacagaggc acccggcgac ggcccgcccg ggaggcagcg gatgagtgag acgatcagcc
     1021 cgctcgagca ggcgaccgtg accagcgtcg gccagctcgt ccaggtgctg cgggcgttcc
     1081 cgccggacac cccggtggag gccggcctcg gctaccgcga cgagaccgac agctaccgga
     1141 cggtgaccgt cagcgcggtg acctcggact ggctcgacgg ccggcctgac gtcaccacgg
     1201 tgatcgtgct cggggagacg ggaagcctcc gcgccccctg acggggacgg gaggtccacg
     1261 gacggggaca tctggaggac acggcatgtc gcagacgcag gcaccaccca tcgagaaggg
     1321 cgcccaggtc aaggtgcggc gcccaggcag cggcgagtcc ggggtctgga aggtgatcgg
     1381 gccggtggcc agctccggtc ctgacgaccc tgcctacgac gtcaccaacc tcatcagcgg
     1441 ccggcagcgg gtcttccggg cgtcgcggct catcgtcctg ggcgccgcca agcgctgagc
     1501 ggcgcgcggt ggcgcccgcc accgcccgcc acccgcgcgt caggccggct gtacccggat
     1561 cccgtcgaag ccgggcgtga agtggccgcc ggagagcatg agcgtgttgg ggccggcgcg
     1621 gagggtcacg gtgacggtgc gcacccccac cgtgtcgaag ctgccggtgc cggcgaagtc
     1681 gaggcggatc cctgcgctgc cgttgacgct gaggtccgcg ctgcgctgct cgcccgacgc
     1741 ataggagatc gccacctggt agcgcccgag gctcggggcc gtcacgccgt tgaactgcag
     1801 cgtcccgccg ttgccgacga agctgacctc ggcgccgccg gagcatccct ggcacgtcac
     1861 gatccgcgcg cctccgccca gcgtattgcc gcccgcctcg gcctcgcgcg agaagcgtag
     1921 cgcgaccgtt gccggcgggg cggtcgtcgg ggcgacggt
//
LOCUS       DASPYY010000013         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2389665, whole genome shotgun sequence.
ACCESSION   DASPYY010000013 DASPYY010000000
VERSION     DASPYY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389665"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VEP73_00160"
     CDS             <1..846
                     /locus_tag="VEP73_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017602554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT24884.1"
                     /translation="VAVVVATRNRVQELLTTLATLERLPERPRVVVVDNGSEDGTASW
                     VRKRHADAELVELDRNLGAAARTVGARLAGEPYVAFSDDDSWWAPGSLARAVEFLERH
                     PRLAVLAARVLVGPEERLDPVCAEMAASPLPPAADLPGPPVLGFVACGAVVRRPAFLQ
                     AGGFHPRFGVGGEEELLAVDLAARGWGLAYVEEVVAHHHPSPSRDPAGRRRVQVRNAL
                     WSAWLRRPPSAVARQAARVLPAALREPAARAGVLEAVRGLPWVLRERRPVGPELEEAL
                     RALGG"
     gene            complement(843..1346)
                     /locus_tag="VEP73_00165"
     CDS             complement(843..1346)
                     /locus_tag="VEP73_00165"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HYT24885.1"
                     /translation="MIYLVRHAHAGRKDDWTAPDALRPLSDSGREEATGLVAGLRDLP
                     VARILSSPATRCLQTVEPLARQRILAIEVDDGLGVHASPARARGLLDDLASTAAVLCT
                     HGELIGQVLRQLAAEGLPLPGSPHWPKGSTWIIDRDGRGRPRASYLPPARVPAAASPD
                     PVGRPAV"
     gene            1509..1991
                     /locus_tag="VEP73_00170"
     CDS             1509..1991
                     /locus_tag="VEP73_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT24886.1"
                     /translation="MSKRPDDGHLAAWRALLEAHAAVTELLAGELEEERALPLGWYEV
                     LLQLADAPGGRLRMQELAQSVLLSKSGLTRLIDRMERAGLVRRQRCPSDRRGSFAVLT
                     QAGRTVFRRAAPVHLRGIEEHFARHLAPAEAEVLRTVLGRVREAARVRSVPARAVPGD
                     "
     gene            2099..2485
                     /locus_tag="VEP73_00175"
     CDS             2099..2485
                     /locus_tag="VEP73_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYT24887.1"
                     /translation="MDEQHARARLEEERERLQRVLDSLQADPETHSDVAAVGELADYD
                     QHPGDVATEVFEHEKNQSLLEQVQAQLTEIEEAFRRIEAGAYGICQACLRPIADERLE
                     AMPATRFCLEDQARAERELGLPGARA"
BASE COUNT          347 a         1032 c         1087 g          353 t
ORIGIN      
        1 gtcgccgtcg tggtggcgac tcgcaaccgt gtgcaggagc tgctcaccac cctcgcgacc
       61 ctggagcggc tgcccgagcg cccccgcgtc gtggtggtcg acaacggctc cgaagacggc
      121 accgcctcgt gggtccgcaa gcggcacgcc gacgccgagc tggtcgagct ggaccgcaac
      181 ctgggcgccg ccgcccgcac ggtgggggcg cggctcgccg gcgagcccta cgtcgcgttc
      241 agcgacgacg actcctggtg ggcgccaggg tcgctcgccc gggcggtgga gttcctcgag
      301 cgccacccga ggctggcggt gctcgccgcc agggtcctcg tcggccccga ggagcggctg
      361 gaccccgtgt gcgcggagat ggccgcgagc ccgctgcccc cagccgccga cctgcccgga
      421 ccgccggtgc tcggcttcgt cgcctgcggt gcggtcgtgc gtcgcccggc gttcctgcag
      481 gcgggcggct tccacccccg cttcggcgtc ggcggggagg aggagctgct cgccgtcgac
      541 ctggcggcgc gcggctgggg cctcgcctac gtggaggagg tggtcgcgca ccaccacccg
      601 tcgccgagcc gcgaccccgc cggacggcgc cgggtccagg tccgcaacgc gctctggtcg
      661 gcctggctgc ggcgtccacc ctcggcggtg gcgaggcagg ccgcccgggt cctgcccgcc
      721 gcgctccgcg agccggccgc gcgggccggc gtcctcgagg ccgtgcgggg cctgccctgg
      781 gtgctgcgcg agcgccgccc ggtcggtccc gagctcgagg aggccctgcg ggcgctcggc
      841 ggctagaccg ccggccgccc gacggggtcg gggctggccg ccgccggcac gcgtgccggc
      901 ggcaggtagc tcgcccgcgg cctgccacgg ccgtcccggt cgatgatcca ggtggagccc
      961 ttgggccagt gaggggagcc gggcagcggc aggccctcgg cggcgagctg gcgcaggacc
     1021 tggccgatca gctcgccgtg ggtgcacagc acggcggcgg tgctggcgag gtcgtcgagc
     1081 agcccgcggg cgcgggcggg gctggcgtgg acgccgaggc cgtcgtcgac ctcgatggcg
     1141 aggatccgct gcctggcgag gggttcgacg gtctgcaggc agcgggtggc cgggctggac
     1201 aggatccggg ccaccggcag gtcgcgcagg cccgccacga gaccggtggc ctcctcgcgg
     1261 ccggagtcgg agagagggcg caacgcgtcg ggcgcggtcc agtcgtcctt ccggccggca
     1321 tgggcgtggc ggaccaggta gatcaacgcg gcctcctccc agctggtccc tgcgcgaaac
     1381 catcgcacat tccgtgcgca caggtcatgc ttgagttgcc agcccggcct cggaccgacc
     1441 cgcccaccat cccggccggc gaggtagttg tcattgcaac gacatgggta ccgggtgata
     1501 cttggagcat gtccaagcgt cccgacgacg ggcacctggc cgcctggcgg gccctgctcg
     1561 aagcgcacgc cgccgtgacg gagctcctcg ccggagagct cgaggaggaa cgcgcgctgc
     1621 cgctcggctg gtacgaggtg ctgctgcagc tcgccgacgc gccaggcggc cgcctgcgga
     1681 tgcaggagct ggcccagtcg gtcctcttgt ccaagagcgg cctgacccgc ctgatcgacc
     1741 gcatggagcg ggccggcctg gtgcgccgcc agcgctgccc gtccgaccgc cgtgggtcgt
     1801 tcgcggtcct cacccaggcc ggcaggaccg tgttccgccg cgccgctccg gtgcacctcc
     1861 gcggcatcga ggagcacttc gcccgccacc tcgcccctgc cgaggccgag gtgctccgca
     1921 cggtcctcgg cagggtgcgc gaggcggcca gggtgcgatc cgttccggct cgggccgttc
     1981 cgggtgatta gctcggaccg atccgggtag aggtggagac tcgcgcaccc tgccgaggac
     2041 cgtgcggcga gcgcatgcgc gaccatgttc ccggacgcga tcagcacgag gagacgccat
     2101 ggacgagcaa cacgcccgag cccgcctcga ggaggagcgc gagcgtctcc agcgggtgct
     2161 cgacagcctc caggccgacc cggagaccca ctcggacgtg gccgccgtcg gcgagctggc
     2221 agactacgac cagcatccgg gcgacgtcgc caccgaggtc ttcgagcacg agaagaacca
     2281 gtccctgctc gagcaggtgc aggcccagct gaccgagatc gaggaggcgt tccgccgcat
     2341 cgaggcgggg gcctacggca tctgccaggc atgcctccgg ccgatcgcgg acgagcggct
     2401 cgaggccatg cctgccaccc ggttctgcct cgaggaccag gccagggccg agcgggagct
     2461 cgggctgccc ggggcgcggg catgaccctc gacgccgacg tcccggccgc cgccgtcact
     2521 cggccccgag cgggactgac ccgctcggga aggagacgcc gcttgtacct ctcgggcgaa
     2581 gctcggtagc atgcgcgcct agcggaccgg cgcacccgcg ctccgtcgcg gccgcgccag
     2641 cgttgagggg ggcgcatgcc agggttcgac gtggtgcgaa ggtggaaggg ccgcgccgtg
     2701 gtcgaccgcg acggcggtga ggtcggcacg atcgtcgagc tctactacga cgaggagacc
     2761 gaccagcccg gctgggcgct ggtggagacc gctgccgccg gggggcggcc gcggctggt
//
LOCUS       DASPYY010000014         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_283647, whole genome shotgun sequence.
ACCESSION   DASPYY010000014 DASPYY010000000
VERSION     DASPYY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283647"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEP73_00180"
     CDS             <1..318
                     /locus_tag="VEP73_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYT24888.1"
                     /translation="PVMVGAGARIENEARVFGPSVLGPGSVVAGGARLERSVLLDGAR
                     LEAGAKVSDSIVGSDVVVGSGAVVGDGTILGDKVVIEDGNVLAGGMRVAPGVHLGPGA
                     IRV"
     gene            complement(379..1185)
                     /locus_tag="VEP73_00185"
     CDS             complement(379..1185)
                     /locus_tag="VEP73_00185"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HYT24889.1"
                     /translation="MVDQHNTKKPAKAPKGGGAQPGAAAKQGGGARQAGTKQAAGKQA
                     AGRQAGTKQGAAAKGKLAKGAAARREAERRRRQRTLAWTAGVVVVVLALVGLILYQGR
                     DTSTKLTRPSAAALATAMKAAGCGDVKQFPQAGRNHIAPDKQPSNWNSNPPTSGDHLG
                     TPLPRGIYADQQDERAAVHNLEHGYVVIQYKNLPGGQVKTLTKLAQSLRGQKLVMMPY
                     GGLPSDGVALTAWQHSQTCQKVNVDVVSAFVDDYMLPGGAKSQAPEPLAA"
     gene            complement(1423..>1669)
                     /locus_tag="VEP73_00190"
     CDS             complement(1423..>1669)
                     /locus_tag="VEP73_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYT24890.1"
                     /translation="EAIARAREAAGSKDVFVMGGADVIRQALRAGHVEELSISIAPVV
                     LGGGKRLFDDFDETVSLEHLRLLQSQFATHITYRVVR"
BASE COUNT          178 a          599 c          627 g          265 t
ORIGIN      
        1 ccggtcatgg tgggcgcggg cgcccgcatc gagaacgagg cccgggtgtt cggcccgtcg
       61 gtgctgggac cggggtcggt cgtcgccggc ggcgcccgcc tcgagcgctc ggtgctgctc
      121 gatggcgccc ggctggaggc gggggccaag gtctccgact cgatcgtggg cagcgacgtc
      181 gtggtcggct ccggcgccgt ggtcggcgac ggcacgatcc tcggcgacaa ggtcgtcatc
      241 gaggacggca acgtgctcgc cggcggcatg cgcgtggcgc cgggcgtgca cctgggcccg
      301 ggcgcgatcc gggtctgacc cggcggccga ggtgggccgc cgggtctcgt cgcctgcgcc
      361 gcgaggcagc gcgggccgtc aggccgccag cggctcgggc gcctgcgact tcgccccgcc
      421 cggcagcatg tagtcgtcga cgaaggcgga cacgacgtcg acgttgacct tctggcacgt
      481 ctgggagtgc tgccaggcgg tcagcgccac cccgtccgac gggagccccc cgtagggcat
      541 catcacgagc ttctgccccc ggaggctctg ggccagcttc gtcagcgtct tcacctggcc
      601 gccgggcagg ttcttgtact ggatcacgac gtagccgtgc tcgaggttgt ggacggccgc
      661 gcgctcgtcc tgctggtcgg cgtagatccc ccgcggcagc ggcgtgccga ggtggtcgcc
      721 agaggtcggc gggttcgagt tccagttgga gggctgcttg tcgggcgcga tgtggttccg
      781 ccccgcctgg gggaactgct tcacgtcacc gcagccggcc gccttcatgg ccgtcgcgag
      841 cgccgcggcg ctggggcggg tgagcttggt cgaagtgtcg cggccctgat agaggatgag
      901 cccgaccagg gcgagcacca ccaccacgac gccggccgtc caggccagcg tccgctgcct
      961 gcggcggcgc tccgcctcgc gccgggccgc cgcgcccttg gcgagcttcc ccttggcggc
     1021 cgcgccctgc ttcgtgccgg cctgcctccc ggcggcctgc ttcccggcgg cctgcttcgt
     1081 gccggcctgc ctggccccgc cgccctgctt ggcggcggcg ccgggctggg cgccgccgcc
     1141 cttcggcgcc ttggcgggct tcttggtgtt gtgctgatca accatgaccg tcggtctcca
     1201 ctgcatcggg gcgggttgcg tcgattctat gcagccaggc ggcggttggc tcggacctga
     1261 ccccggtgac cccggtccgg ggctccggca cggtctcggt gaggtccgcg caccctgggt
     1321 gcaggcggcc gtggggttct gcacgagggc cgcgggatag tctgcgccca tgagcacgtt
     1381 cgagacgttg ctgacccgtg gcagccagca ggccgccgcc ggtcagcgca cgacgcgata
     1441 ggtgatgtgc gtcgcgaact gtgactggag caggcgcagg tgctcgaggc tcaccgtctc
     1501 gtcgaagtcg tcgaacagac gcttcccgcc gccgagcacg accggcgcga tcgagatcga
     1561 cagctcttcc acgtgtccgg cgcgcagcgc ctggcggatg acgtccgccc cgcccatgac
     1621 gaacacgtcc ttgctgccgg ccgcctcgcg cgcccgagcg atcgcctcg
//
LOCUS       DASPYY010000015         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2250153, whole genome shotgun sequence.
ACCESSION   DASPYY010000015 DASPYY010000000
VERSION     DASPYY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250153"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VEP73_00195"
     CDS             <1..1203
                     /locus_tag="VEP73_00195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYT24891.1"
                     /translation="GERLDPAGSRVVDCSGLVVSPGFADLHVHFREPGREDAETIETG
                     SRAAALGGFTAVCPMPNTDPVADNAGVVEMVAARGREVGLVDVFPVGAVTVGQRGAEL
                     AELGAMARSAASVDCFSDDGHPIREARLLRLALEYARAFDAVIADHAEDASLTDGAQM
                     HEGQVSAILGLQGWPAAAEEMVVARDLLLAELTGGRLHLCHLSTAGAVDLVRSAKARR
                     VRVTAEATPHHFTLTDEAARSYDPVFKVNPPLREKADVEAVREGLADGTLDAIATDHA
                     PHAREDKEVEWAAAPPGMLGLETALALAVTELVEPGFLTLPQLVERLTAGPARCRRLP
                     GHGGPVAPGTPANLAVFDPAARWTVDTRSLASRARNTPYQGRELTGKVVHVLLRGTFT
                     VTDGKATR"
     gene            1200..>1568
                     /locus_tag="VEP73_00200"
     CDS             1200..>1568
                     /locus_tag="VEP73_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24892.1"
                     /translation="MNWLLGGLTVVALGGLAAWVMLGAIRRTARRQQTGLPALPECPG
                     ASPAGPVSPAAGDRAVLEAAGARYLGTTYAPSTVRRFSGRGLLGRAVVDLVLDPAGLR
                     VDRGAAGAWCIPAEAVRGAEV"
BASE COUNT          167 a          633 c          583 g          185 t
ORIGIN      
        1 ggggagcgcc tcgacccggc cgggtcgcgc gtcgtcgact gctcggggct ggtggtcagc
       61 cccggcttcg ccgacctgca cgtgcacttc cgggagccgg gcagggagga cgccgagacg
      121 atcgagaccg gctcgagggc ggccgccctc ggcggcttca ccgccgtctg cccgatgccc
      181 aacaccgacc cggtggccga caacgccggg gtggtcgaga tggtggccgc ccgcgggcgc
      241 gaggtcgggc tcgtcgacgt cttcccggtc ggcgcggtca ccgtcggcca gcgcggggcc
      301 gagctggccg agctgggggc gatggcgcga tcggcggcct ccgtcgactg cttctccgac
      361 gacggccacc cgatccgcga ggcgcgcctg ctccgcctcg cgctcgagta cgcgcgcgcg
      421 ttcgacgcgg tgatcgccga ccacgccgag gacgcctcgc tgaccgacgg cgcgcagatg
      481 cacgaggggc aggtctcggc catcctcggc ctgcagggct ggcccgccgc cgccgaggag
      541 atggtcgtcg cccgcgacct gctgctcgcc gagctgaccg gggggcgcct gcacctctgc
      601 cacctgtcca cggccggcgc ggtcgacctg gtgcgatccg cgaaggcgcg ccgcgtgcgg
      661 gtcaccgccg aggccacgcc ccaccacttc accctcaccg acgaggccgc gcgctcctac
      721 gacccggtgt tcaaggtgaa cccgcccctg cgcgagaagg ccgacgtcga ggcggtccgc
      781 gagggcctcg ccgacgggac cctcgacgcg atcgccaccg accacgcccc ccacgcccgc
      841 gaggacaagg aggtggagtg ggcggcggcc ccgcccggga tgctcgggct cgagaccgcg
      901 ctcgcgctcg ccgtcaccga gctggtcgag cccggcttcc tcacgctgcc gcagctcgtc
      961 gagcggctca ccgccggccc ggcccgctgc cggcggctgc ccggccacgg cggcccggtc
     1021 gcgccgggga ccccggccaa cctcgcggtc ttcgacccgg ccgcgcgctg gacggtcgac
     1081 acccggtcgc tcgcgagccg cgcccgcaac acgccctacc agggccgcga gctcaccggc
     1141 aaggtcgtcc acgtcctgct ccgggggacg ttcaccgtca ccgacggcaa ggcgacccgg
     1201 tgaactggct cctggggggt ctcaccgtcg tggcgctcgg cgggctcgcc gcgtgggtca
     1261 tgctcggcgc gatccggcgc accgcgcggc ggcagcagac cggcctcccg gccctcccgg
     1321 agtgcccggg cgcctccccg gccgggccgg tctcgcccgc ggcgggcgac cgtgccgtgc
     1381 tcgaggcggc cggcgcccgc tacctcggga ccacctacgc gccttccacg gtgcgccggt
     1441 tctccggccg cggcctgctc ggccgcgcgg tcgtcgacct cgtcctcgac ccggccggcc
     1501 tgcgggtcga ccggggggca gccggtgcct ggtgcatccc ggccgaggcg gtgcggggcg
     1561 ccgaggtc
//
LOCUS       DASPYY010000016         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_143521, whole genome shotgun sequence.
ACCESSION   DASPYY010000016 DASPYY010000000
VERSION     DASPYY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143521"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VEP73_00205"
     CDS             <1..868
                     /locus_tag="VEP73_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT24893.1"
                     /translation="ARAAPAAPAARAAPGRAGRCAVLLVELRKLVVRPRTWVTIALLA
                     GLPTLVAVFLKVTGVGPRPGQGPAFLAEVLSNGALFPAAALALVLPVFLPVAVSVVAG
                     DAVAGEASAGTLRYLLTRPVGRTRLLLVKLVVVIVFVFLAVLVVAGVAFAVGAWLFGV
                     KPLPSVSGATITGPDAYFRTTVTVAYVAWSMLGVGAIALFASTVTDSPLAAALGALAA
                     LVTSEVLDLLDAAAAVKPYLPTHYWLAFVDLFRTPILWRGVGRGFAIQGVYIAVFLGA
                     AWANFATKDVKS"
     gene            complement(944..1738)
                     /locus_tag="VEP73_00210"
     CDS             complement(944..1738)
                     /locus_tag="VEP73_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT24894.1"
                     /translation="MLRSLSPRRRLLAVAVVAAVAVGAAGLALALRPGTTPKALPGAV
                     VPVLLVHGYDGTPAEFDRLAVLLAASGRPVVRVALPARGTGDIEASARVVARAAGRTG
                     AARVDLVGYSAGGIVVRAFLDLPGRAATARHVVLLGAPNHGTEIAGLAAALDPRLCPV
                     ACSQLAPTSSLLARLNADGTPPGPDYTSIWTSRDETVTPPDSAVLEGARNVRVQDVCA
                     SSGVGHGGLVTDPAVLGLVVRALDGALDARPGPSDCAPLEALGARA"
     gene            1837..>3479
                     /locus_tag="VEP73_00215"
     CDS             1837..>3479
                     /locus_tag="VEP73_00215"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HYT24895.1"
                     /translation="MADKYVYDFEEGNRDMKYVLGGKGANLAEMTNMGLPVPHGFTAT
                     CQACNAYRAAGQRFPEGMLDEVAEHLRALEENMGRRLGEPGDPLLVSVRSGAPFSMPG
                     MMDTVLNVGLNDQSVDGLAKQTGSERFAWDSYRRLLQMFGKTVMGVDGDRFEEAIEHA
                     KEAKGVKNDVDLDEGDLRRLVEDFKRVIASDTGREFPQDPQEQLKLGIQAVFGSWDNK
                     RARDYRRKNKIDDALGTAVNVQSMVFGNKGDDSGTGVAFTRNPANGEAAPYGDYLANA
                     QGEDVVAGIRNTLKLEEMGDVHPEAWEELQGHMHTLEEHYRDMCDIEFTVEQGKLWLL
                     QTRVGKRTAFAEWIMAYDMLEQGLIDVDTAVLRLDANRLEELFKKVIAEGGGAEPIAA
                     GLNASPGAAVGKAVFTADEAQAWAERGEPVILVRRETTPDDYHGMIRSQGILTSAGGT
                     NSHAAVVARGEGIPAVCGADAIRVDRPGRKFTAGQTTVAEGDWVTIDGFTGKVYAGQL
                     ELADSPIERARSGDAEARKEKLWQAYERFMTRADEARRL"
BASE COUNT          472 a         1256 c         1325 g          426 t
ORIGIN      
        1 ggcccgggcg gccccggcgg ccccggcggc ccgggcggcg ccggggcggg cggggaggtg
       61 cgctgtgctg ctggttgagc tgcgcaagct cgtcgtgcga ccccggacct gggtgaccat
      121 cgcgctgctg gccggcctgc ccaccctggt cgccgtgttc ctgaaggtga cgggcgtcgg
      181 gccccggccc ggccaggggc ccgccttcct ggccgaggtc ctgtcgaacg gcgccctgtt
      241 cccggccgcc gccctggccc tcgtgctgcc ggtgttcctg cccgtggcgg tgtcggtggt
      301 ggccggcgac gcggtcgccg gcgaggccag cgccggcacg ctgcgctacc tgctcacccg
      361 gccggtcggg cgcacccggc tgctgctggt gaagctcgtc gtcgtcatcg tgttcgtctt
      421 cctcgccgtg ctcgtggtcg cgggcgtcgc cttcgccgtg ggcgcctggc tcttcggggt
      481 caagccgctg ccgtcggtgt cgggggcgac gatcaccggc ccggacgcct acttccgcac
      541 gacggtgacc gtcgcctacg tcgcctggtc gatgctcggg gtgggggcga tcgccctgtt
      601 cgcgtcgacc gtgaccgact cgccgctggc cgccgccctc ggcgccctcg ccgcgctcgt
      661 cacctccgag gtcctcgacc tgctggacgc cgccgccgcg gtcaagccct acctgcccac
      721 gcactactgg ctcgcgttcg tcgacctgtt ccgcaccccg atcctgtggc gcggcgtcgg
      781 ccgcggcttc gcgatccagg gcgtctacat cgccgtcttc ctcggcgccg cctgggccaa
      841 cttcgccacc aaggacgtca agagctgacc tcgccgccgc cgggcccccg tcaggcccgg
      901 ccgcccccgg ccccccgtca ggccaggccg cggccggccc gcgtcaggcc cgggcgccga
      961 gggcctcgag aggggcgcag tcggacgggc cgggccgggc gtcgagggcc ccgtccagcg
     1021 cccggacgac caggccgagg acggccgggt cggtgaccag gccgccgtgg ccgacgccgg
     1081 acgaggcgca gacgtcctgg acccgcacgt tgcgcgcgcc ctcgagcacc gccgagtccg
     1141 gcggggtcac ggtctcgtcc ctcgacgtcc agatgctcgt gtagtcgggg ccggggggcg
     1201 tcccgtcggc gttcaggcgg gccagcagcg aggaggtcgg cgcgagctgg ctgcacgcca
     1261 cggggcacag ccgcgggtcg agcgccgcgg cgagcccggc gatctcggtg ccgtggttcg
     1321 gcgcgccgag caggacgacg tggcgcgcgg tggcggcccg gccgggcagg tcgaggaacg
     1381 cccgcacgac gatcccgccg gccgagtagc cgaccaggtc caccctggcc gcgccggtcc
     1441 ggccggcggc gcgcgccacg accctggccg aggcctcgat gtccccggtg ccccgcgccg
     1501 gcagggcgac gcgcaccacc ggccggccgg aggccgcgag cagcacggcg agccggtcga
     1561 actcggccgg cgtcccgtcg tagccgtgca ccagcagcac gggcaccacg gcgccgggga
     1621 gcgccttggg cgtcgtgccc ggccggagcg cgagggcgag cccggcggcc cccacggcga
     1681 ccgccgcgac gaccgcgacc gcgagcaggc ggcggcgcgg gctcagcgag cgcagcatgg
     1741 gaccattgtg ccgcccgccg ggcgggtagg gtggtcgtgc ccgccatcgt tcgcggacct
     1801 gcactacgct acggccaacc attcagagga ggcgcaatgg cggacaagta cgtgtacgac
     1861 ttcgaagagg gcaaccggga catgaagtac gtcctcggcg ggaagggggc aaacctcgcc
     1921 gagatgacga acatgggcct gcccgtgccg cacgggttca ccgccacctg ccaggcgtgc
     1981 aacgcctacc gggcggcggg gcagcggttt cccgagggca tgctggacga ggtcgccgag
     2041 cacctgcggg cgctggagga gaacatgggg cgccggctcg gcgagccggg cgacccgctg
     2101 ctcgtgtcgg tccggtcggg cgcgccgttc tcgatgccgg ggatgatgga cacggtcctg
     2161 aacgtcgggc tgaacgacca gagcgtcgac ggcctcgcca agcagaccgg ctccgagcgg
     2221 ttcgcgtggg actcctaccg gcggctgctg cagatgttcg gcaagacggt catgggtgtg
     2281 gacggcgacc ggttcgagga ggccatcgag cacgccaagg aggccaaggg cgtcaagaac
     2341 gacgtcgacc tggacgaggg cgacctgcgc cggctggtgg aggacttcaa gcgggtgatc
     2401 gcctccgaca ccgggcggga gttcccgcag gacccgcagg agcagctgaa gctggggatc
     2461 caggccgtgt tcggctcctg ggacaacaag cgggcccggg actaccggcg caagaacaag
     2521 atcgacgacg cgctcggcac cgcggtgaac gtccagtcga tggtgttcgg caacaagggc
     2581 gacgactccg gcacgggggt ggcgttcacc cgcaacccgg ccaacgggga ggccgcgccc
     2641 tacggcgact acctggccaa cgcgcagggc gaggacgtcg tcgccgggat ccgcaacacc
     2701 ctcaagctcg aggagatggg ggacgtccac ccggaggcgt gggaggagct ccaggggcac
     2761 atgcacaccc tggaagagca ctaccgggac atgtgcgaca tcgagttcac cgtcgagcag
     2821 ggcaagctgt ggctgctgca gacccgggtc ggcaagcgga ccgcgttcgc cgagtggatc
     2881 atggcctacg acatgctcga gcagggcctg atcgacgtcg acaccgcggt cctgcgcctc
     2941 gacgcgaacc ggctcgagga gctgttcaag aaggtcatcg ccgagggcgg gggcgccgag
     3001 ccgatcgccg ccggcctgaa cgcctcgcca ggcgctgcgg tgggcaaggc cgtgttcacc
     3061 gccgacgagg cgcaggcgtg ggccgagcgg ggcgagccgg tcatcctcgt ccgccgcgag
     3121 accaccccgg acgactacca cggcatgatc cggtcccagg gcatcctgac ctcggcgggc
     3181 gggacgaact cccacgccgc cgtggtcgcc cgcggcgagg gcatcccggc ggtgtgcggc
     3241 gccgacgcca tccgggtcga ccggccgggc aggaagttca cggccggcca gacgacggtc
     3301 gccgagggcg actgggtcac gatcgacggg ttcaccggca aggtctacgc gggccagctc
     3361 gagctcgccg actcgccgat cgagcgggcc cgctcgggcg acgccgaggc gcgcaaggag
     3421 aagctctggc aggcctacga gcggttcatg acccgggccg acgaggcgcg ccggctcaa
//
LOCUS       DASPYY010000017         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1407792, whole genome shotgun sequence.
ACCESSION   DASPYY010000017 DASPYY010000000
VERSION     DASPYY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407792"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEP73_00220"
     CDS             complement(<1..194)
                     /locus_tag="VEP73_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYT24896.1"
                     /translation="MSGKRDFLTVDDVSPAELAALLDGADRHKADRRPRTTLAGRTVA
                     LVFEKPSTRTRVSFEVAVHEL"
     gene            complement(191..586)
                     /locus_tag="VEP73_00225"
     CDS             complement(191..586)
                     /locus_tag="VEP73_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT24897.1"
                     /translation="MAPNRVLVVEDDPSVRGLIQTILEDEALDVVLASDGEEGLRLAR
                     SIHPRVVLLDVMMPGLGGPEVIRRLRDDDGSLPFAVLVVTGAAEAIASLRGELGPDAV
                     LEKPFDIMTLAARVRSLAERGPAAQRSSP"
     gene            complement(712..1920)
                     /locus_tag="VEP73_00230"
     CDS             complement(712..1920)
                     /locus_tag="VEP73_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYT24898.1"
                     /translation="MDYTVAGVEGGICAPRGFRAAGVDAGLVPGGGPDLALLVSQQSA
                     VAAGRFTSHRLPSAPVRWSRRQLEGGQARAVLVHAGAANAVTGPEGEADAAALAGLCA
                     RALGCPPQEVLLLGTGRIGVRLGVEHAAEGAEKAVAVLTRRASDDVGRALGASGGVVR
                     QHAVAVAWSEGEVRIGAVAKASHPFAPSFATTLAVLTTDATVERGVLDGILARAVGRS
                     FERVLVDQGPGMADAVVMLANATAAAPVLAAGSEGALAFESALGQLCELLAEGLAMRV
                     PGARKLVVVTVHGAAEHADALAAARAIAGSVAVRAALAAGQAAWEAVLDALGGAGVDL
                     DPGRVGVRFGPVTVVEDGRVASHDERAAAAVAAKAQVDLAVDLGVGPAAATVTTTDLL
                     PEALGFTGSR"
     gene            complement(2013..>2548)
                     /locus_tag="VEP73_00235"
     CDS             complement(2013..>2548)
                     /locus_tag="VEP73_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYT24899.1"
                     /translation="EGLVGVLGIEGVAYRAEEPMPFHPGRCAGVFLGGQPVGLLGQLH
                     PRVAAALDLPAATYAAELWVGALLEAAPAMRPAPTPSPYPELSFDVAFLVPPGVPAGS
                     LDAVLREAGGELLVRLTLFDAYEGSPLPPGHRNLAYRVALQARDRTLTDAEARAVRDR
                     MEALARERLQAVLRAAE"
BASE COUNT          319 a          993 c          973 g          263 t
ORIGIN      
        1 agctcgtgca cggccacctc gaacgacacc cgggtccggg tggagggctt ctcgaacacg
       61 agcgcgaccg tccggcccgc gagggtggtg cggggccggc ggtcggcctt gtgcctgtcg
      121 gccccgtcga gcagggcggc cagctcggcc ggggacacgt cgtcgacggt caggaagtcg
      181 cgcttgccgc tcatgggctg ctcctttgcg ccgccgggcc gcgctcggcc agcgaccgga
      241 cccgggcggc gagcgtcatg atgtcgaagg gcttctcgag cactgcgtcc gggccgagct
      301 cgccccgcag cgaggcgatg gcctcggccg cgccggtgac cacgaggacg gcgaagggca
      361 gggagccgtc gtcgtcgcgg aggcgccgga tgacctcggg gccgccgagc ccgggcatca
      421 tgacgtcgag cagcacgacc cgcgggtgga tggaccgtgc cagcctgagc ccttcctcgc
      481 cgtcggaggc gagcaccacg tcgagggcct cgtcctcgag aatcgtctgg atgaggccgc
      541 gcacgctcgg gtcgtcctcg accacgagca cacggttggg agccatcagc ggggtgcctt
      601 tctgaatgcc gtcacgagat gccgcgtgct ccggcggcct gccgtccgct cgcgagcgaa
      661 caagtctgcc atgcggtccc gaccccaggc caccatccgg cgccgccatg gctaccggga
      721 gccggtgaag ccgagcgcct cggggaggag gtcggtcgtg gtgacggtcg ccgccgccgg
      781 accgacgccg aggtcgacgg cgaggtcgac ctgggccttg gcggcgaccg ccgcggcggc
      841 ccgctcgtcg tgggaggcca cccgcccgtc ctcgacgacg gtgaccgggc cgaagcggac
      901 gccgacccgg ccagggtcga ggtccacgcc ggcccccccg agggcgtcga ggaccgcctc
      961 ccacgccgcc tgcccggcgg cgagggcggc ccggaccgcg accgacccgg cgatcgcgcg
     1021 tgccgcggcg agcgcgtcgg cgtgctcggc ggccccgtgc acggtcacca cgacgagctt
     1081 gcgggcacct gggacgcgca tggcgagccc ctcggcgagc agctcgcaga gctggccgag
     1141 ggcggactcg aacgcgagcg ccccctcgga gccggccgcg aggacgggcg ctgccgcggt
     1201 cgcgttcgcg agcatcacga ccgcgtcggc catgcccggc ccctggtcga cgaggacgcg
     1261 ctcgaaggag cgcccgaccg cccgggcgag gatgccgtcg aggacgcccc gctcgacggt
     1321 cgcgtcggtg gtgagcacgg ccagggtggt cgcgaacgac ggggcgaacg ggtgcgacgc
     1381 cttggcgacc gcgccgatgc ggacctcccc ctccgaccag gcgacggcga ccgcgtgctg
     1441 ccgcacgacc cccccggacg cgccgagcgc cctgcccacg tcgtccgaag cgcgccgggt
     1501 gagtacggcg accgccttct cggcgccctc ggccgcgtgc tcgacgccga gccgcacgcc
     1561 gatcctgccg gtgccgagga gcagcacctc ctggggcggg cagccgagcg cccgggcgca
     1621 caggccggcg agcgctgccg cgtcggcctc cccctccggg ccggtgaccg cgttggccgc
     1681 gccggcgtgg accagcacgg cgcgggcctg gccgccctcg agctggcggc gcgaccagcg
     1741 caccggggcc gacgggagcc ggtgcgaggt gaagcggccg gccgcgaccg cgctctgctg
     1801 gctgaccagc agggcgaggt ccgggccgcc gccgggcacc agcccggcgt cgacgccggc
     1861 ggcccggaag ccgcgcgggg cgcagatgcc gccctcgacc cccgcgaccg tgtaatccac
     1921 cacctggctc ccttccatcg ccttggcaag agcctacggg tcccgggggc gctgcacacc
     1981 cccccgcggg cgctcggcgc cagcgctccc ctctactcgg ccgcgcgcag caccgcctgg
     2041 agccgctcgc gggcgagggc ctccatccgg tcccggacgg cccgcgcctc ggcgtccgtc
     2101 agggtccggt cgcgtgcctg cagggcgacc cggtaggcga ggttgcggtg cccgggtggc
     2161 aacggcgagc cctcgtaggc gtcgaacagg gtcagccgga cgagcagctc cccgcccgcc
     2221 tcccgcagga cggcgtcgag cgacccggcc ggcacgccgg gcggcaccag gaacgccacg
     2281 tcgaacgaca gctccggata cggcgagggc gtcggggccg ggcgcatggc cggcgccgcc
     2341 tcgaggaggg ccccgaccca cagctcggcc gcgtacgtcg ccgccggcag gtcgagcgcg
     2401 gcggccaccc gcgggtggag ctgcccgagg agcccgaccg gctgcccgcc gaggaacacc
     2461 ccggcgcacc ggcccgggtg gaacggcatc ggctcctcgg cccggtaggc gacgccctcg
     2521 atcccgagga cgcccaccag cccctcca
//
LOCUS       DASPYY010000018         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1407817, whole genome shotgun sequence.
ACCESSION   DASPYY010000018 DASPYY010000000
VERSION     DASPYY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407817"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VEP73_00240"
     CDS             <1..520
                     /locus_tag="VEP73_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HYT24900.1"
                     /translation="VTLAVDGGTKVPLRVQVFAKGQASPAFETGFTEVQFRTPDASVF
                     RFAPPPGAKVTEQRPGTEPKAVDHGRKRAATAPAGGPGVIGSGWTSVVVARGVTTGSV
                     TGTAKALLQAATPVHGAFGSGRLLRSPLFSVLLTDDGRLYLGAVTPDALTAAAAQPAA
                     AARPLVGQGGTP"
     gene            517..1503
                     /locus_tag="VEP73_00245"
     CDS             517..1503
                     /locus_tag="VEP73_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT24901.1"
                     /translation="MTATTAARAALPPTAAGGPPPALATRGLTKRFRGGQVAVADLDL
                     LVPPGSVFGFLGPNGSGKTTTIRMVLGLVAPTGGRAELLGAPMPAGAAAVLPRVGALV
                     EGPAFHPYLSGRANLERLDAADALADPRTTGRRVDRALERVGLAAAARKRYRAYSMGM
                     RQRLGLAAALLHPRELLVLDEPTNGLDPQGTREVRALVRELAAEGATVFLSSHLLSEV
                     EQICTHVAVLRSGRLVAQGSLAELGAAAEPRLAVETSEPGLAAEVLASLGCRDVQRHA
                     DAADAVLDEVAPERACAALVAAGVGVRGLAVRRQSLEDTYVSLTGEGFDVDE"
     gene            1490..>2040
                     /locus_tag="VEP73_00250"
     CDS             1490..>2040
                     /locus_tag="VEP73_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT24902.1"
                     /translation="MSTSSASVERVATAPPVGRRRPARGVGRFYRSELGLVFGRRRNL
                     VLLGVLALVPVLIGVSIKLASSSPGPGEGPPFLDRIAGNGLFLALTSLVVVLPLFLPL
                     AVGVVSGDAVAGEANLGTLRYLLVVPVRRGRLLAVKYGALIAFCLAAALVVAAAGLAT
                     GLVLFPGGRVALLSGATIALPAAL"
BASE COUNT          192 a          790 c          790 g          268 t
ORIGIN      
        1 ggtgaccctc gcggtcgacg gcggcaccaa ggtgccgctg cgggtgcagg tgttcgccaa
       61 gggccaggcg agcccggcgt tcgagaccgg cttcaccgag gtccagttcc gcacccccga
      121 cgcgagcgtg ttcaggttcg cgcccccgcc gggcgccaag gtgaccgagc agcgcccggg
      181 caccgagccg aaggcggtcg accacggccg caagcgcgcc gccacggcgc cggccggcgg
      241 cccgggcgtc atcgggtcgg gctggacgtc ggtggtggtc gcccgcggcg tgaccaccgg
      301 gtcggtcacg gggacggcga aggcgctcct gcaggcggca acgcccgtcc acggcgcctt
      361 cggcagcggg cggctgctcc ggtcgccgct gttcagcgtg ctgctcaccg acgacggccg
      421 cctgtacctc ggcgcggtga cgcccgacgc gctgaccgcg gcagccgcgc agccggccgc
      481 ggccgcgagg cccctggtgg gccagggcgg caccccgtga cggccacgac cgccgcgcgg
      541 gcggccctcc cgcccaccgc ggcggggggg ccgccgcccg cgctcgccac ccgcggcctc
      601 accaagcggt tccgcggcgg ccaggtcgcg gtcgccgacc tcgacctcct cgtgccgcca
      661 gggagcgtgt tcgggttcct cggcccgaac gggtccggca agaccacgac gatccgcatg
      721 gtgctcggtc tcgtggcccc gaccggtggc agggccgagc tgctgggcgc gccgatgccg
      781 gcgggcgccg cggcggtcct cccgagggtg ggcgcgctcg tcgaggggcc cgcgttccac
      841 ccgtacctct ccggccgggc gaacctcgag cgcctcgacg cggccgacgc cctggccgac
      901 ccgcgcacga ccgggcgccg ggtcgacagg gcgctggagc gggtcgggct cgcggccgcc
      961 gccaggaagc gctaccgggc ctactcgatg gggatgcgcc agcggctcgg gctggccgcg
     1021 gcgctgctgc acccgcgcga gctgctcgtc ctcgacgagc cgaccaacgg cctcgacccc
     1081 cagggcaccc gcgaggtgcg cgcgctcgtc cgcgagctgg ccgcggaggg ggcgaccgtg
     1141 ttcctctcct cgcatctcct gagcgaggtg gagcagatct gcacccacgt cgcggtcctg
     1201 cggagcgggc ggctggtcgc ccaggggagc ctcgccgagc tgggggcggc ggccgagccg
     1261 cgcctcgcgg tcgagacgag cgagccgggc ctggccgcgg aggtgctcgc ctccctcggc
     1321 tgccgcgacg tgcagcggca cgccgacgcc gccgacgccg tgctcgacga ggtcgcgcct
     1381 gagcgcgcgt gcgccgccct cgtcgcggcc ggcgtcggcg tgcggggtct cgcggtccgg
     1441 cgccagtcgc tcgaggacac ctacgtctcg ctgaccgggg aggggttcga tgtcgacgag
     1501 tagcgcatcc gtcgagcggg tggccacggc cccaccggtg ggccgccgcc ggcccgcccg
     1561 tggggtcggg cgcttctacc gctccgagct ggggctggtg ttcgggcgtc gccgcaacct
     1621 cgtgctcctc ggcgtcctgg cgctcgtccc cgtgctcatc ggtgtctcga tcaagctcgc
     1681 gtcgtcctcg ccgggccccg gggagggacc gccgttcctc gaccggatcg ccggcaacgg
     1741 gctgttcctc gccctcacgt cgctcgtggt ggtgttgccg ctgttcctgc cgctcgcggt
     1801 cggggtcgtc tccggggacg cggtcgccgg agaggcgaac ctcggcacgc tgcgctacct
     1861 gctggtcgtg ccggtgcggc ggggccggct gctcgcggtg aagtacggcg ccctcatcgc
     1921 gttctgcctg gccgccgcgt tggtcgtcgc cgccgccggc ttggcgacgg gcctcgtgct
     1981 gttcccgggc ggccgggtgg cgctgctctc cggcgccacc atcgccctgc cggccgcgct
//
LOCUS       DASPYY010000019         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1969759, whole genome shotgun sequence.
ACCESSION   DASPYY010000019 DASPYY010000000
VERSION     DASPYY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969759"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VEP73_00255"
     CDS             <1..381
                     /locus_tag="VEP73_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYT24903.1"
                     /translation="LGARPWQPGSGGLGVKLAAVPSGLPGALETVWATAAAHGLAARA
                     AAHAATGILWAGLDRAEPAFVNEARERLAGRATLVVAEAPLEAKRVVDAWGPAGDALP
                     LMRRVKERFDPAGLLSPGRFAGGI"
     gene            384..1760
                     /locus_tag="VEP73_00260"
     CDS             384..1760
                     /locus_tag="VEP73_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYT24904.1"
                     /translation="MADTQPTEAGGQVLPAFDDHHPPEKALIDDCVHCGFCLPTCPTY
                     LLWGEEMDSPRGRIYLMDIGLADAPLNDEMVRHFDQCLGCMACVTACPSGVQYDKLIE
                     ATRQQVERRHPRTRRDRAFRALVFSLFPYPRRLRLLRGPLRLAQRAGLRTLLRRAGVE
                     RMLPDRLGSMQTLLPDLGPMEKVPEVTPPAGAPRARAGILVGCVQSVFFPQVNAATAR
                     VLAAEGCEVVAPPGQGCCGALSMHAGREAEAQRFARRTIDVFEAAGVDQVVVNAAGCG
                     SSMKEYGHLLRDDPRYAERAARFSAKVRDVSEFLAELEPAAERHPLPVKVAYHDACHL
                     AHAQGVRVPPRQGLRAVPGLEVREIDEAEICCGSAGIYNLVEPEPARALGDRKAGNVL
                     ATGADLLVTSNPGCLLQIRASLERRGERLPMAHVVEVLDASIRGEAAESLLARIGGRM
                     EGNETTRP"
BASE COUNT          235 a          727 c          697 g          233 t
ORIGIN      
        1 ctcggcgccc ggccctggca gccggggtcg ggcgggctcg gcgtcaagct cgccgcggtg
       61 ccgagcgggc tccccggcgc cctggagacg gtctgggcga ccgcggccgc gcacgggctc
      121 gccgcccggg cggccgcgca tgccgccacc gggatcctct gggccgggct cgaccgcgcc
      181 gagcccgcgt tcgtgaacga ggcccgcgag cggctcgcgg gccgggcgac cctcgtcgtc
      241 gccgaggcgc cgctcgaggc caagcgggtg gtggacgcct gggggccggc cggcgacgcg
      301 ctgccgctca tgcgccgggt gaaggaacgc ttcgacccgg cgggcctcct cagcccgggc
      361 cgattcgccg gagggatctg aacatggccg atacccagcc cacggaggcc ggcgggcagg
      421 tcctgccggc gttcgacgac caccacccgc ccgagaaggc gctgatcgac gactgcgtgc
      481 actgcggctt ctgcctgccg acgtgcccga cgtacctcct gtggggcgag gagatggact
      541 cgccccgcgg ccgcatctac ctgatggaca tcgggctcgc cgacgccccc ctgaacgacg
      601 agatggtgcg ccacttcgac cagtgcctcg gctgcatggc gtgcgtcacc gcctgcccct
      661 cgggcgtcca gtacgacaag ctcatcgagg ccaccaggca gcaggtcgag cgccgccacc
      721 cgcgcacgcg ccgcgaccgc gccttccggg cgctggtgtt ctcgctcttc ccctacccga
      781 gacggctccg cctgctgcgc gggccgctgc ggctcgccca gcgggccggc ctgcgcacgc
      841 tcctgcggcg ggccggggtg gagcggatgc tgcccgaccg cctcgggtcg atgcagaccc
      901 tgctgcccga cctcggcccg atggagaagg tgcccgaggt gaccccgccc gccggggcgc
      961 cgcgcgcacg ggcgggcatc ctcgtcggct gcgtccagtc ggtcttcttc ccgcaggtca
     1021 acgcggccac cgcccgtgtc ctggccgccg aggggtgcga ggtcgtggcc ccgcccgggc
     1081 agggctgctg cggggcgctc agcatgcacg ccggccggga ggcggaggcg cagcggttcg
     1141 cgcgccgcac catcgacgtc ttcgaggcgg caggggtcga ccaggtcgtc gtcaacgccg
     1201 ccggctgcgg ctcctcgatg aaggagtacg gccacctcct gcgcgacgac ccccggtacg
     1261 ccgagcgcgc ggcgcggttc tcggccaagg tcagggacgt ctcagagttc ctggcggagc
     1321 tggagccggc cgccgagcgc caccccctcc cggtcaaggt cgcctaccac gacgcctgcc
     1381 acctcgcgca cgcgcagggc gtccgcgtgc cgccgaggca gggcctccgc gccgttcccg
     1441 gcctcgaggt ccgcgagatc gacgaggcgg agatctgctg cgggtcggcc ggcatctaca
     1501 acctggtcga gcccgagccg gcgcgcgcgc tgggcgaccg caaggccggc aacgtgctcg
     1561 ccaccggcgc cgacctgctg gtcacgtcga accccgggtg cctgctgcag atccgggcga
     1621 gcctcgagcg ccggggcgag cggctcccga tggcgcacgt cgtcgaggtg ctcgacgcct
     1681 cgatccgcgg cgaggcggcc gaatcgctgc tcgcacgcat aggtgggcga atggagggga
     1741 acgagacaac cagaccatga cgtgcgctgg gagggtgggg gcccgctcag cccgggctcc
     1801 ccacgcgggg ggaaggagtg gtggcaccga tgcccgtgct cgcctccagc tacacccagc
     1861 actacacgcc cgtcgcccgc aacatcctcc tc
//
LOCUS       DASPYY010000020         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2250689, whole genome shotgun sequence.
ACCESSION   DASPYY010000020 DASPYY010000000
VERSION     DASPYY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250689"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEP73_00265"
     CDS             complement(<1..374)
                     /locus_tag="VEP73_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24905.1"
                     /translation="MADDLLHPDDRPDPAARCRADLAGYLRDLPAPELADLLDGLPDP
                     VTVDPIGALAARGPASARLPDDWPGHPDSEFMRRRSLREVVADRRAARAARRAGPPPG
                     ATLARPARQLAVCDPACAGSGGR"
     gene            complement(364..2193)
                     /locus_tag="VEP73_00270"
     CDS             complement(364..2193)
                     /locus_tag="VEP73_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYT24906.1"
                     /translation="MRTNDTTPDTPSGPPADPRPAWERIAATWLAREVDAGQPVTPEQ
                     LARETSVTPAYTRDLLQVLRAQRARDPALAELRARLVGDRLTTLYVTREVHGGERLDP
                     AALAAELGTTTTVARQWLHGLRAQQTSEQGMGALLEPASHGRPSAEQLAGLAAHFADG
                     GHQRAAVTGRPADPEALEVEVERRYWTREARAGQRLNPVALARELGTEPHLVAAQLRE
                     LRAGPATARERIEQLWHAQQQDPGARPLSSSRLAHRLGVTDSYVRHVTWRLRTREGIA
                     PLADRLAATRERLSTPPPPLATRADPERDWRLDAACRDADPELFFPDQGQLPQVAAAK
                     EVCAGCAVRQPCLDTALHGPLRRDDQHGIFAGTTAHDRTRLRGRPAMAEGTRFLTDRA
                     AAEEALALANRVSLDRAARELGVSKQALRHAFDHHGLPQPQVFQGGPQRSPAYHDRDT
                     AEQAWRRATEVGINQTRKELGISDRALRNAWAHHGLGLPPRPTSRPPTAAGGRLDPTF
                     VALNPEVIPVRPGSEAERFARVRRAEQLATLGADVVVELNTESRCRQQARVAAITRRA
                     QRAQTLARQREGRSERRSAERADRDRRAHPPRAQEREVLPDGR"
     gene            complement(2197..>2640)
                     /locus_tag="VEP73_00275"
     CDS             complement(2197..>2640)
                     /locus_tag="VEP73_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24907.1"
                     /translation="SGLADLSALELGAKLIGDRQTLERARSMGSDGRHSLNESVGWRP
                     LLPVEDLRRLRPGEGVLVYGHLRPTPVRLRPYYTPAEQRRRVRLEDRAERRLARAEAH
                     RERATAHARERTERARPGAPERPGWAGRLRVLRPGGRDARQREEG"
BASE COUNT          250 a          912 c         1158 g          320 t
ORIGIN      
        1 cgaccgccgg atcccgcgca ggcggggtca cagacggcca gttggcgagc cggtcgggcc
       61 agggtggcgc cgggcggcgg cccagcgcgg cgggcggcgc gggcggcgcg ccggtcggcg
      121 accacctccc gcagcgaccg ccgccgcatg aactcgctgt caggatggcc gggccagtcg
      181 tcagggagcc tggcggacgc cgggccgcgc gcggccagcg cgccgatcgg gtcgacggtg
      241 accgggtcgg gcagcccgtc cagcaggtcg gccagctcgg gcgcgggcag atcgcggagg
      301 tagccggcca ggtcggcgcg gcaccgcgcg gccgggtcgg gccggtcgtc agggtgcagc
      361 aggtcatcgg ccatcgggga gcacctcccg ctcctgggct cggggagggt gggcgcggcg
      421 gtcacggtcg gcccgctcgg cgctgcggcg ctcgctgcgg ccttcgcgtt ggcgggcgag
      481 ggtctgggcg cgctgggcgc ggcgggtgat ggcggccacg cgggcttgct ggcggcagcg
      541 gctttcggtg ttcagctcga cgaccacgtc ggcgccgagg gtggcgagct gctcggcgcg
      601 gcggacccgc gcgaaccgct ccgcctccga gcccgggcgg accggtatca cctcggggtt
      661 gagcgcgacg aacgtcgggt cgagccgacc acctgcggcc gtgggcggcc ggctggtggg
      721 gcgggggggt aggccgaggc cgtggtgggc ccaggcgttg cgcaaggccc ggtcggagat
      781 accgagctcc ttgcgggtct ggttgatgcc gacctcggtg gcgcgccgcc aggcctgctc
      841 ggcggtgtcg cggtcgtggt aggcgggcga gcgctgcggc ccgccctgga acacctgcgg
      901 ttgggggagg ccgtggtggt cgaaggcgtg ccggagggcc tgcttggaga cgccgagctc
      961 gcgggcggcc cggtcgaggc tgacccggtt ggccagcgcc agcgcctcct cggcggcggc
     1021 gcggtcggtg aggaaccggg tgccctcggc catcgcgggc cggccgcgga ggcgggtgcg
     1081 gtcgtgggcg gtggtgccgg cgaagatgcc gtgctggtca tcccggcgca gggggccgtg
     1141 gagggcggtg tcgaggcagg gctggcggac cgcgcagccg gcgcagacct ccttggccgc
     1201 cgcgacttgg gggagctggc cttggtcggg gaagaacagc tccgggtcgg cgtcgcggca
     1261 ggcggcatcc agccgccagt cgcgctccgg gtccgcccgg gtggctagtg gcggtggtgg
     1321 ggtgctgagg cgctcgcggg tggcggccag ccggtcggcc agcggggcga tcccttcgcg
     1381 ggtgcgcagc cgccaggtga cgtggcgcac gtagctgtcg gtcacgccca ggcggtgggc
     1441 gagccggctg gagctcaggg gccgggcgcc ggggtcctgc tgctgggcgt gccagagctg
     1501 ctcgatccgc tcccgggcgg tggccggccc ggcccgcagc tcgcggagct gcgcggcgac
     1561 caggtggggc tcggtgccga gctcgcgggc gagcgcgacc gggttgagcc gctggccggc
     1621 gcgggcctcg cgggtccagt agcgccgctc cacctcgacc tcaagcgcct ccgggtcggc
     1681 ggggcgaccg gtgaccgcgg cgcgctggtg gccgccgtcg gcgaagtggg cggccaaccc
     1741 ggcgagctgc tcggccgagg ggcggccgtg gctggccggc tcgagcagcg cccccatccc
     1801 ctgctcgctg gtctgctggg cgcgcaggcc gtgcagccac tgccgcgcga ccgtggtggt
     1861 ggtgcccagc tcggctgcca gggcggcggg gtcgagccgc tcgccgccgt ggacctcccg
     1921 ggtgacgtac agggtggtga gtcggtcgcc gaccagccgg gcgcgcagct cggcaagcgc
     1981 cgggtcgcgg gcgcgttggg cgcgcaggac ctgaagcagg tcgcgggtgt aggcgggggt
     2041 gacgctggtc tcgcgggcga gctgctcggg ggtgacgggc tggccggcgt cgacttcgcg
     2101 ggccagccag gtggcggcga tccgctccca ggccgggcgc gggtcggcgg gcggccccga
     2161 gggagtatcg ggcgtggtgt cgttggtgcg cacgggtcac ccctcctccc gctgccgagc
     2221 atcccggccg ccaggccgca gaacccggag ccgcccagcc caccccggcc gttcaggcgc
     2281 acccgggcgg gcgcgttcgg tgcgttcccg ggcgtgggcg gtggcgcgct cccggtgggc
     2341 ctccgcgcgg gccaggcgcc gctcggcgcg gtcctcgagc cggacgcggc ggcgctgctc
     2401 cgcgggggtg tagtagggcc gcaggcggac cggggtgggg cgcaggtgcc cgtagaccag
     2461 cacgccctcc cccggccgca gccgccgcag gtcctccacc ggcagcagcg gccgccagcc
     2521 caccgactcg ttcagcgagt ggcggccgtc cgaccccatc gagcgggcgc gctcgagggt
     2581 ctgccggtcg ccgatcagct tggcgccgag ctcgagcgcg gagaggtcgg cgaggccgga
//
LOCUS       DASPYY010000021         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_143853, whole genome shotgun sequence.
ACCESSION   DASPYY010000021 DASPYY010000000
VERSION     DASPYY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143853"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VEP73_00280"
     CDS             complement(<1..697)
                     /locus_tag="VEP73_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYT24908.1"
                     /translation="MREVGLSATEFGPDGFLPGEPDAMTQVLDHHGLRPSVGSPRCCS
                     TRPGTTDPVPEVERLLQGYDASGAGVLVLSAVTGQEGYDTRPGLDEAGWRTLLANLDR
                     LTALAAEHAVRAVLHPHVGTMVENGDEVRRVLEGSSIPLCLDTGHRFIGGTDPAELAR
                     QAPGRVQDGRLTYTEGVRDGMYRPLWSGDVDVPAIVGYLRARGYGGWYVLEQDTILTG
                     GPRGDGPAADVWAS"
     gene            complement(811..2076)
                     /locus_tag="VEP73_00285"
     CDS             complement(811..2076)
                     /locus_tag="VEP73_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT24909.1"
                     /translation="MTSISTSTNSSATGDIGVGLISVGWMGKLHSRAYQALPSVYPEL
                     GLRPWLVHAADTAADRVEYARDVLGYRKGSTDYRDVLADPDVDVVSICAPNALHHEIG
                     LAAAEAGKPFWIEKPVGRDARETGEVAAAVRKAGIATSIGYNYRHAPAVEHVRELVAQ
                     GALGRITNVRAVFFTGYASEPKGALSWRFRRDLAGTGALGDLLSHVVDLVSYVVGPIT
                     EVTALTSTVYAERPILPMGSATHFAVIEDGEMGPVENEDYAATLVRFAPSSRGPGAVG
                     TLEASRTIVGPQCGLGFELYGTDGSAVWNFEQMNELRICLGRGGPHQGYTTVLGNAHF
                     GDYGNFQPGPGIGMGYDDLKVVEAKKFLVAVTGGERRNSTIEDAHAVAEVISAAAASA
                     ASGTWQAVPAVPGATFGGEPAPAQGPTGD"
BASE COUNT          264 a          765 c          784 g          295 t
ORIGIN      
        1 cactggccca cacgtcggcc gccgggccgt cgcccctggg ccccccggtc aggatcgtgt
       61 cctgctccag tacgtaccag ccgccgtagc cgcgggcgcg caggtacccg acgatggcag
      121 gcacgtccac gtcgcccgac cagagcgggc ggtacatccc gtcacgcacc ccttcggtgt
      181 aggtcagcct gccgtcctgg acccgcccgg gcgcctgcct ggccagctcg gccgggtcgg
      241 tgccgccgat gaaccggtgc ccggtgtcca ggcacagggg gatcgaggag ccttcgagca
      301 cccgccggac ctcgtcgccg ttctcgacca tggtgcccac gtgcggatgc agcacggccc
      361 gcacagcgtg ctcggcggcc agcgccgtca ggcggtccag gttggccagc agggtccgcc
      421 agccggcctc gtccagcccc gggcgggtgt cgtagccctc ctggccggtg acggccgaca
      481 gcaccaggac gcccgcgccg gaggcgtcgt agccctggag caggcgctcg acctctggca
      541 cggggtcggt cgtgcccggc cgcgtggagc agcagcgggg tgaacccacc gacggccgca
      601 ggccgtggtg gtccaggacc tgggtcatcg cgtcgggctc gccgggcagg aagccgtcgg
      661 gcccgaactc ggtcgcgctc aggccgacct cgcgcatctc ggccagcacc cggccggtag
      721 gcgggcagga accggtccag cgacttggac accccggccc gctcggcgac gtcgcggatc
      781 gtcggccggc gcggcgggcc ggcgacctcc tcagtcaccg gtcgggccct gcgcgggcgc
      841 cggctcgccg ccgaaggtgg cgcccggaac ggccgggacc gcctgccagg taccgctggc
      901 ggcggaggcg gcggcggccg agatgacctc ggccacggcg tgggcgtcct cgatggtgga
      961 gttgcggcgc tccccgccgg tcacggcgac gaggaacttc ttggcctcga cgaccttgag
     1021 gtcgtcatag cccatcccga tgcccgggcc gggctggaag ttgccgtagt cgccgaagtg
     1081 ggcgttgccg agcaccgtgg tgtagccctg gtgagggccg ccgcggccca ggcagatccg
     1141 cagctcgttc atctgctcga agttccagac ggccgagccg tcggtgccgt agagctcgaa
     1201 gccgagcccg cactggggcc cgacgatcgt ccgcgacgcc tccagcgtcc ccacggcccc
     1261 cggcccgcgc gagcttggcg cgaagcggac cagggtggcg gcgtagtcct cgttctccac
     1321 cgggcccatc tcgccgtcct cgatgaccgc gaagtgggtg gccgacccca tcggcaggat
     1381 cggccgctcg gcgtagacgg tgctggtcag cgcggtgacc tcggtgatcg ggccgaccac
     1441 gtagcttacg aggtcgacga cgtggctgag caggtcgccg agggcgccgg tgccggccag
     1501 gtcacggcgg aagcgccacg acagcgcccc cttgggctcg gaagcgtagc cggtgaagaa
     1561 caccgcgcgg acgttggtga tgcgcccgag cgcaccctgg gccaccagct cgcggacatg
     1621 ctccacggcc ggggcgtggc ggtagttgta gccgatggac gtggcgatcc cggccttccg
     1681 gacggccgcg gcgacctcgc cggtctcccg cgcgtcccgc ccgaccggct tctcgatcca
     1741 gaagggcttg cctgcctcgg cggccgccag gccgatctcg tggtgcaggg cgttcggtgc
     1801 gcagatcgac accacgtcca cgtccgggtc ggccaggacg tcacggtagt cggtgctccc
     1861 cttgcggtag ccgaggacgt cgcgggcgta ctcgacgcgg tcggcggcgg tgtcggcggc
     1921 gtgcaccagc cagggccgca gcccgagctc cgggtagacc gacgggaggg cctggtaggc
     1981 tcggctgtgg agcttgccca tccagccgac gctgatcaga cccacgccga tgtcgccggt
     2041 cgcgctgctg ttcgtgctgg tgctgatgct ggtcatgtgc ggcctccccg cttgctgggg
     2101 tgggttgt
//
LOCUS       DASPYY010000022         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_424938, whole genome shotgun sequence.
ACCESSION   DASPYY010000022 DASPYY010000000
VERSION     DASPYY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424938"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1817)
                     /locus_tag="VEP73_00290"
     CDS             complement(<1..>1817)
                     /locus_tag="VEP73_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYT24910.1"
                     /translation="VPPATDDAAASPEVGAPAPRRWWRLALHGLAPAALVVEAWLVLQ
                     YLLKQVTRPLWYDEQWRAYHFTLSGPEFYRELRLTNAPLAAGWVALEKLSTGLLGDRE
                     VPLRLPVVVAYLATGVVTYALARRFVGAPGGFLVAAGVLANASILQLGLQLKPFTIEV
                     LAGELAMLLWLDADRPGRGLAGRLARYAGMGLAAVVGTAAAFVVGPLLLVDAARLARE
                     RTLRRLLPPLLAGVVVLVHLKAFVLLQTGQSRSAYWNAYFVPHEPFGDKVGFTLHGLA
                     GFVPGVFLGDLGAARSGITRSLAFGSAATSMLVPFMVVALALGVVAALRSGPGRTLLA
                     VLGLSLDAQLLASSMRLWPFGFARTNLFLVPFAYLLAGVGVAWPLSALLRLATGGRRR
                     ARSWLEAGPAARWAMRRLWALWLAAPDRLAPLVAPERVRRLVGRPLSRGLVAAALAVL
                     AALAAVNLVSVGEVSVRQLRLVGQASDGLPYGGELRGLVRTVRLRDTPTDVVVHVGSM
                     STKGWHYYMQPGYDGYSRAVSPRSAIGPDRTVTTDDPVVLWRFLAAHRGAAQAFVVVQ
                     QGESKQAIDTVYRVLNRAGYRDLVNRDQAGVTMVLQLAR"
BASE COUNT          278 a          667 c          706 g          166 t
ORIGIN      
        1 ggcgggcgag ctggagcacc atcgtgacgc cggcctggtc gcggttgacg aggtcccggt
       61 accctgcccg gttgagcacc cggtagaccg tgtcgatggc ctgcttggac tctccctgct
      121 gcaccaccac gaaggcctgc gccgcgccgc ggtgcgcggc gaggaaccgc cagaggacga
      181 ccgggtcgtc ggtggtgacg gtgcggtccg gcccgatcgc ggaccgcggg ctgaccgccc
      241 gggagtagcc gtcgtagccg ggctgcatgt agtagtgcca gcccttggtg ctcatgctcc
      301 cgacgtgcac caccacgtcg gtcggggtgt cacgcaggcg gacggtgcgg accaggccgc
      361 gcagctcgcc cccgtacggc aggccgtcgc tggcctgccc gacgagccgc agctggcgga
      421 cgctcacctc gccgaccgag acgaggttga ccgcggcgag cgcggcgagg acggcgagcg
      481 ccgccgcgac cagcccgcgg gagagggggc ggcccacgag ccgcctgacc cgctcgggcg
      541 ccaccagggg agcgagccgg tccggcgcgg ccagccagag ggcccacagc cgccgcatcg
      601 cccagcgcgc ggccggcccg gcctcgagcc agctccgcgc ccgccggcgg ccgccggtgg
      661 ccagccggag cagggcggac agcggccagg ccacgccgac cccggccagc agataggcga
      721 agggcacgag gaacaggttg gtgcgcgcga agccgaacgg ccacagccgc atcgacgagg
      781 cgaggagctg cgcgtccagc gacagcccca ggacggcgag gagggtgcgg ccgggccccg
      841 accgcagcgc cgccaccacc ccgagggcca gggcgaccac catgaacggg acgagcatcg
      901 acgtggccgc gctcccgaag gcgagcgagc gggtgatgcc gctcctcgcc gccccgagat
      961 cgccgaggaa cacgccgggc acgaagccgg ccaggccgtg cagggtgaac ccgaccttgt
     1021 cgccgaacgg ctcgtggggg acgaagtacg cgttccagta ggcgctccgg gactggccgg
     1081 tctgcaggag cacgaacgcc ttcaggtgga ccaggacgac caccccggcg agcagggggg
     1141 gcaggaggcg gcggagcgtc cgctcgcggg cgagccgggc ggcgtccacc agcagcagcg
     1201 ggccgaccac gaaggccgcg gccgtcccca cgaccgcggc cagccccatc ccggcatacc
     1261 tcgccagccg cccggccagc ccccgcccgg gccggtcggc gtcgagccag aggagcatcg
     1321 ccagctcgcc ggcgaggacc tcgatcgtga acggcttgag ctgcaggccg agctggagaa
     1381 tgctcgcgtt ggcgaggacg cctgcggcca ccaggaagcc gcccggcgcg ccgacgaagc
     1441 ggcgggcgag cgcgtaggtc accacgccgg tcgcgaggta ggcgacgacc acggggaggc
     1501 gcagggggac ctcgcggtcg ccgaggaggc cggtcgacag cttctcgagc gcgacccagc
     1561 ccgccgcgag cggtgcgttc gtcagccgga gctcgcggta gaactcgggc ccggagaggg
     1621 tgaagtggta cgcccgccac tgctcgtcgt accacagggg tctcgtgacc tgcttcagga
     1681 ggtactggag gacgagccac gcctcgacga ccagcgcggc cggcgcgagc ccgtgcagcg
     1741 ccagccgcca ccagcgccgg ggggccggcg cgccaacctc gggggacgcg gcggcgtcat
     1801 cggtcgccgg cgggacc
//
LOCUS       DASPYY010000023         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1688816, whole genome shotgun sequence.
ACCESSION   DASPYY010000023 DASPYY010000000
VERSION     DASPYY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688816"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VEP73_00295"
     CDS             complement(<1..657)
                     /locus_tag="VEP73_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT24911.1"
                     /translation="MTFEGKVAVVTGGGSGIGRAASLLLASRCAAVAVADIDRDAAAA
                     VAAEVTSAGGRAVAVGVDVTDAARVRAMVAESERALGPIDVLVSNAGWDEVAPFVDTD
                     EAFWDRVIAINFRGHLATTHAVLPGMIERGRGRIVNVASGAAYLPGASKTAYAASKAA
                     VNRFSETLGKQLEPHGIPVFPISPGLVRTEMTEPNFPDDAPWTPPECAPRLVRALASG
                     R"
     gene            complement(654..1370)
                     /locus_tag="VEP73_00300"
     CDS             complement(654..1370)
                     /locus_tag="VEP73_00300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT24912.1"
                     /translation="MIAAAELMAVVLSRELRDGETAIMGAVSALPLAACRVAQLTHAP
                     DLWFITGGSGAVNAHPRHLPESSCDQQLLAAETSLPLPEIVLLEGRGDVVDVFFAGGL
                     QIDARGNCNLVAVGDWTRPRLRGPGGVGLSFLPRAGRAVIYTTSHTPRTFVEEVDFVS
                     GPGLTAALVVTPLCTFGFHRRTERMRLRTVHRGVTVEQVRAATGFEVAAPDPVPQTPD
                     PTPEEVAALSRVDPLGVLRA"
     gene            complement(1367..>1858)
                     /locus_tag="VEP73_00305"
     CDS             complement(1367..>1858)
                     /locus_tag="VEP73_00305"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT24913.1"
                     /translation="GAGGLPFMALPRGLELSDVPSVNPECYALVEDPFSGESYLAVPA
                     LRPDVALIHAAEADARGNCGFKGSCFTDRLMALAARHVIVQVERLVSTDEVSAYPAGS
                     ALPGFLVDAVVVAPGGCLPTAAHGEYGYDESALRRYLELARDEAGFVEYVERHVLGRV
                     GVA"
BASE COUNT          261 a          705 c          662 g          230 t
ORIGIN      
        1 gcgccccgag gcgagcgcgc ggacgagccg gggcgcgcac tcgggtggcg tccagggcgc
       61 gtcgtccggg aagttcggct ccgtcatctc cgtgcgcacg aggccggggc tgatcgggaa
      121 gaccgggatg ccgtggggct cgagctgctt cccgagcgtc tcgctgaacc ggttcacagc
      181 cgccttgctc gctgcgtacg ccgtcttcga cgcgccgggg aggtaggccg ccccgctcgc
      241 gacgttgacg atccggccgc ggccgcgctc gatcatgccc ggcaggacgg cgtgcgtggt
      301 cgccaggtgg ccccggaagt tgatcgcgat cacgcggtcc cagaacgcct cgtcggtatc
      361 cacgaaaggc gcgacctcgt cccagccggc gttggagacc aggacgtcga tcggtcccag
      421 ggctcgctcc gattccgcga ccatcgcccg cacgcgcgcg gcgtcggtca cgtccacccc
      481 caccgcgacc gcccggccgc cggccgaagt cacctccgct gcgaccgccg ccgccgcgtc
      541 gcggtcgatg tccgccaccg ccaccgcggc gcatctgctc gccagcagca gggacgcggc
      601 ccggccgatg ccgctgccgc ccccggtgac cacggcgacc ttgccttcga acgtcacgct
      661 cggaggactc ccagcgggtc cacccggctc agcgccgcca cctcctccgg ggtggggtcg
      721 ggcgtttggg ggaccggatc cggcgccgcc acctcgaagc cggtcgcggc ccgcacctgc
      781 tcgacggtca cgccgcgatg cacggtgcgc agccgcatcc gctcggtccg gcggtggaag
      841 ccgaatgtgc agagcggggt caccaccagg gcggcggtca gacccgggcc gctcacgaag
      901 tcgacctctt cgacgaaggt gcggggtgtg tgcgaggtcg tgtagatgac cgcccgcccg
      961 gcgcggggca ggaaggagag gccgacgccc cccggaccgc gcaaccgggg ccgtgtccag
     1021 tccccgaccg cgaccaggtt gcagttgccg cgggcgtcga tctgcaggcc gcccgcgaag
     1081 aagacgtcga ccacgtcgcc gcggccctcc aggaggacga tctcgggcag gggcaggctg
     1141 gtctcagccg ccagcagctg ctggtcgcat gaggactccg gcagatgccg ggggtgcgcg
     1201 ttgactgcgc cgctgccgcc cgtgatgaac cagaggtcgg gggcatgcgt cagctgggcg
     1261 acccgacacg ccgccagcgg cagagcgctg acggcgccca tgatcgccgt ctcgccgtcc
     1321 cgcagctcgc ggctcaggac caccgccatc agctccgctg ccgctatcac gcgaccccca
     1381 cccgccccag cacgtggcgt tccacgtact cgacgaagcc cgcctcgtcc cgggccagct
     1441 ccaggtaacg ccggagtgcc gactcgtcgt agccgtactc gccgtgggcg gcggtcggca
     1501 ggcagccgcc cggcgccacc accacggcgt cgaccaggaa cccgggcagc gcgctgccgg
     1561 cgggataggc ggagacctca tcggtggaga ccaggcgctc gacctgcacg atcacgtgcc
     1621 gcgccgccag cgccatcagg cggtcggtga agcagctccc tttgaaaccg cagttgccgc
     1681 gagcatccgc ctcggcggcg tggatcaatg ccacgtccgg ccgcagcgcc ggcacggcaa
     1741 ggtaggactc gccgctgaag gggtcctcga ccagcgcgta gcactcgggg ttcacgctgg
     1801 ggacgtccga gagctccagc ccgcgcggca gcgccatgaa gggcaggccg ccggcgcc
//
LOCUS       DASPYY010000024         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_144096, whole genome shotgun sequence.
ACCESSION   DASPYY010000024 DASPYY010000000
VERSION     DASPYY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144096"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>1695
                     /locus_tag="VEP73_00310"
     CDS             237..>1695
                     /locus_tag="VEP73_00310"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HYT24914.1"
                     /translation="MRWHWYFSALALAGGACLAAYTWYVWRRRQASAGASLTVVLAAA
                     GWWALAYALELGATRLPAKLLWGDAKYLGICLLPVAWFAFIMQYTGRDRWVNRRVLTA
                     LAVPPAALIVLLANRATHDLVRWYPPSAAGDPDAIAQVGPLFWPFFAYASLVVWGCTA
                     LFVWTLTRVSRLYWRQSLLLIVTVLLPFVANVVHNLNVGPFGRVEPTPFLFVLTGAVL
                     VWGIFRFRLLDLAPIARSSVFETMLDGVLVLDPYRRVVNLNPAAERAIGVPAAAAVGR
                     PAEALLAVEPSVLDGADDPALPAEVVLGDSRHELTTAPLRDRSGRETGRVVVLRDVTE
                     RHHAHERLVQLDEERRWLLGRVVRAQEEERRRIAGDIHDDAIQTIAAARLRLAVFRNR
                     LQDPEQRRQLEEAETAVSSSLERLRTLVFDLRPAMLDEVGLGAALRQYLAETTGQSSF
                     KAQLRDDLAQEPPAEVRVIAYRIAQEAITNVRMHAR"
BASE COUNT          194 a          665 c          598 g          238 t
ORIGIN      
        1 ggcggtggtg ccccgcgacg tgcccccgcg cgccgtggtc gccggcaacc ccgcccgcca
       61 gacccgcgac gtcgacgacc tcacctgccc ggccgggctc ccccaccgtc cctacccgcc
      121 ggcctgaccc gggcgtccgg gacggcgccg ccgggagcgg cttcgcgtga ccgccccgaa
      181 tgtcatatga ttcgctaccc gtcggaaccg gtcatccggc acgaaacgag gtctgcgtga
      241 ggtggcactg gtacttcagc gcgctcgcgc tggcgggcgg tgcctgcctt gccgcctaca
      301 cctggtacgt ctggcgccgc cggcaggcgt cggccggggc cagcctgacc gtggtgctgg
      361 cggcggccgg ctggtgggcc ctggcctacg ccctcgagct gggcgccacc cgcctgcccg
      421 ccaagctgct ctggggcgac gccaagtacc tcggcatctg cctgctcccg gtcgcctggt
      481 tcgcgttcat catgcagtac accggcaggg accggtgggt gaaccggcgc gtgctgaccg
      541 ccctcgcggt cccgcccgcg gcgctgatcg tcctgctcgc caaccgggcc acccacgacc
      601 tggtgcgctg gtacccgccg tcggcggccg gcgacccgga cgccatcgcg caggtcgggc
      661 cgctgttctg gcccttcttc gcctacgcca gcctggtcgt gtggggctgc accgcactgt
      721 tcgtgtggac gctgacgcgc gtgtcgcggc tgtactggcg gcagagcctc ctgctcatcg
      781 tcaccgtgct gctgcccttc gtcgccaacg tcgtgcacaa cctgaacgtc ggcccgttcg
      841 gacgggtcga gccgaccccg ttcctgttcg tcctgaccgg cgcggtgctg gtgtggggca
      901 tcttccgctt ccgcctgctc gacctggcgc cgatcgcgcg cagctcggtc ttcgagacga
      961 tgctggacgg cgtgctcgtg ctcgacccct accggcgggt cgtgaacctc aacccggcgg
     1021 ccgagcgggc catcggcgtg ccggccgccg ccgcggtggg ccggccggcc gaggcgctcc
     1081 tcgccgtcga gccgtccgtg ctcgacggcg ccgacgaccc ggccctgccc gcggaggtcg
     1141 tcctcggcga cagccgccac gagctgacca ccgccccgct gcgggaccgg agcggccggg
     1201 agaccggccg cgtggtcgtc ctgcgcgacg tcaccgagcg ccaccacgcg cacgagcgcc
     1261 tggtccagct cgacgaggag cgccgctggc tgctcggccg ggtggtgcgc gcccaggagg
     1321 aggagcggcg ccgcatcgcc ggcgacatcc acgacgacgc gatccagacc atcgcggccg
     1381 cccggctccg gctcgccgtg ttccgcaacc gcctccagga ccccgagcag cgccggcagc
     1441 tcgaggaggc cgagaccgcc gtgtcgtcca gcctggagcg gctgcgcacg ctggtgttcg
     1501 acctccgccc ggccatgctc gacgaggtcg ggctcggcgc ggcgctgcgg cagtacctgg
     1561 ccgagacgac cgggcagagc agcttcaagg cccagctccg cgacgacctg gcgcaggagc
     1621 cgcccgccga ggtgcgcgtg atcgcctacc gcatcgcgca ggaggcgatc accaacgtgc
     1681 gcatgcacgc gcggg
//
LOCUS       DASPYY010000025         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2531798, whole genome shotgun sequence.
ACCESSION   DASPYY010000025 DASPYY010000000
VERSION     DASPYY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531798"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEP73_00315"
     CDS             <1..536
                     /locus_tag="VEP73_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24915.1"
                     /translation="KPVTDLGDSLRVMVMAAGQSTDLPLEPQFEVGEWGTPGDYRAFF
                     IPTSPGRYTFHFHGTVKGQKVSQAFTSSPSTFSDVEDPAKVMFPVKEPSSGELAQRLD
                     REVPRLSVAVQSAQAASRTAEQRARDAAGQARLLALAGAVTGLLGLAVAVVAFRRKPT
                     VRVVTERRREPASAGRA"
     gene            541..>1647
                     /locus_tag="VEP73_00320"
     CDS             541..>1647
                     /locus_tag="VEP73_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYT24916.1"
                     /translation="MRLRRPPAAVALLAGLWLLATGPAAGAHGVLRASDPPGGASLAA
                     PPHAVTLSFSEAPDLGLSSVHVLDTAGRALERGRPEPVPGRSLALRVPLGGLPAGTYT
                     VSWKVVSRVDGHTTGGAYVFGVGVPPVAPPGARQVMTTRADAPSPVAVAGRWSFYWGL
                     ALLVGAAASGLAVFDRRLPGRPALLLTGAVVVAAGGLAAMVAGARSGAGVPLGTLLGS
                     RVGHLLAWQAGALATAALAVVALLRRPGRAWPLAAVGVTAAGGLLAHALAGHAAAPSS
                     LRGVNLATQWAHLLAVGAWVGGLPWLLAGLRGRDRAGQAAAAVRFSRLATACLAAVAV
                     TGFVRAAEELGGWARLLDTAFGRVLDVKLALFGAL"
BASE COUNT          141 a          665 c          629 g          212 t
ORIGIN      
        1 gcaagccggt caccgacctc ggcgacagcc tccgcgtgat ggtcatggcc gcggggcagt
       61 cgacggacct gccgctcgag ccacagttcg aggtgggcga gtggggcacc cccggcgact
      121 accgggcgtt cttcatcccg acctcgcccg gccgctacac cttccacttc cacgggaccg
      181 tcaagggcca gaaggtcagc caggccttca cctcctcgcc gtcgaccttc tccgacgtcg
      241 aggatccggc caaggtcatg ttcccggtca aggagccgag cagcggcgag ctcgcgcagc
      301 ggctcgaccg cgaggtgccc cggctgagcg tcgccgtgca gagcgcccag gccgcgagcc
      361 ggaccgccga gcagcgcgcc agggacgccg ccggccaggc ccggctgctc gccctcgccg
      421 gcgcggtcac gggcctgctc ggcctggccg tcgccgtcgt cgccttcagg cgcaagccga
      481 ccgtccgggt cgtgaccgag cggcgccggg agcccgcgag cgccggaagg gcgtgacccg
      541 atgcggctgc ggcgcccccc cgccgccgtc gccctgctcg ccggcctgtg gctcctcgcc
      601 accgggccgg cggcaggcgc tcacggtgtc ctccgcgcgt ccgacccgcc cggcggggcg
      661 tcgctggccg cgccccccca tgccgtcacc ctctcgttca gcgaggcgcc ggacctcggc
      721 ctgtcgagcg tccacgtgct cgacacggcc gggcgcgccc tcgagcgcgg ccggcccgag
      781 ccggtccccg gcaggtctct ggccctccgc gtcccgctcg gcggcctgcc cgccggcacc
      841 tacacggtga gctggaaggt ggtgtcgcgg gtggacggcc acaccaccgg cggggcctac
      901 gtgttcggcg tgggcgtacc cccggtggcc ccgccgggcg cccggcaggt catgaccacg
      961 cgggcggacg cgccgtcgcc ggtcgcggtc gccggccgct ggagcttcta ctggggcctc
     1021 gccctgctcg tcggcgccgc ggcaagcggc ctggccgtgt tcgaccggcg cttgcccggc
     1081 cggcccgccc tgctgctcac cggagccgtc gtggtggccg ccggcgggct cgcggccatg
     1141 gtcgccggcg cacggtccgg cgccggcgtg ccactcggca cgctgctcgg ctcgcgcgtg
     1201 ggccacctgc tcgcctggca ggcgggggcc ctggccacgg ccgcgctcgc cgtggtcgcg
     1261 ctgctgcggc gccccggccg ggcctggccc ctggccgcgg tcggcgtgac cgcggccggc
     1321 gggctgctgg cgcacgcgct ggccgggcac gcggccgcgc cgtcgtcgct gcggggggtc
     1381 aacctggcga cccagtgggc gcatctcctc gcggtcggcg cctgggttgg cgggctgccg
     1441 tggctcctgg ccggcctgcg cggccgggac cgggcggggc aggccgccgc cgccgtccgc
     1501 ttctcccggc tcgccacggc atgcctggcc gccgtggcgg tcaccgggtt cgtccgggcg
     1561 gccgaggagc tcggcgggtg ggcgcggctg ctcgacaccg ccttcggccg cgtgctggac
     1621 gtcaagctgg cgctgttcgg cgcgctg
//
LOCUS       DASPYY010000026         4725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_425215, whole genome shotgun sequence.
ACCESSION   DASPYY010000026 DASPYY010000000
VERSION     DASPYY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425215"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VEP73_00325"
     CDS             complement(<1..688)
                     /locus_tag="VEP73_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HYT24917.1"
                     /translation="MTSRPLPLLTDDEVARAVPAGDEAGAGALSTERGNLPLEAVDVD
                     ADVAGLLARVVVTQTFSNPHPEPLEATYVFPLPDRAAVTEFRMEVAGRVVDGVLEERG
                     AARATYDRAVAEGRRASIAEEERPDVFTMRVGNLMPGERASVRLAMAGPLPYADGEAT
                     FRFPLVVAPRYVPGVPLGGEPAGDGTALDTGAVPDASRITPPVLLPGFPNPVRLSIAV
                     RVDPGGLPLAR"
     gene            complement(685..1368)
                     /locus_tag="VEP73_00330"
     CDS             complement(685..1368)
                     /locus_tag="VEP73_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYT24918.1"
                     /translation="MGTTAFWTLDELTRGVAAALAVDYDGQASGRVRDVPDQRAIRYY
                     TTLGLVDRPAAMRGRTALYGPRHLLQLVAIKRLQARGLSLAAVQAELAGVGEADLERI
                     ARVPPAPPGEEAPRASGARASRASGARASRASGEDAPRRFWAASPAPAPSSPGPPGPL
                     MRPAPAARLQGIRLSEEATLLIGAHRPVDDDDVRALQAAARPLLEALAARGLAEPAPD
                     GPTTGRDLP"
     gene            complement(1400..2689)
                     /gene="thrC"
                     /locus_tag="VEP73_00335"
     CDS             complement(1400..2689)
                     /gene="thrC"
                     /locus_tag="VEP73_00335"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYT24919.1"
                     /translation="MTAAEHDRLTPAPAADGVSLRCRGCRTPYPPELRYACPNCLGPL
                     DVEYDGDAVDPRLARASIEAGPASIWRYGAFLPSRPGAGDLSPGLTPLVRTPRLAEAL
                     AIPGELWCKDDTRNPTNSFKDRVVAVAVAKAREFGLSTVACASTGNLAGATAAAAAAQ
                     GLDCVVVIPADLEAGKIASAATFGATVVAVEGSYDHANRLSAQAAEAFGWGFVNVNLR
                     PWYAEGSKTVGLEVAEQLGWSLPDHVVVPIASGALLSKVHQGFGTLVAAGLVAGTPVH
                     VHGAQSDGCPPVAAAFAEGRDDVRPVRPTGIVKSLGIGDPADGVEALEVVRSTGGRVE
                     AVTDEEVVDAIGLLARTTGIFGETAAGVTIATLRRLAQRGAFRPGERIVALVTGHGLK
                     TREVLDGRDGATITVRPNIDALEAALDPVRPDLVSGS"
     regulatory      complement(2749..2867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2978..3976
                     /locus_tag="VEP73_00340"
     CDS             2978..3976
                     /locus_tag="VEP73_00340"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HYT24920.1"
                     /translation="MAELDTWDWFGNRTFHYGQFEPVSRLRAARERSGTTVSICLPAR
                     NEAATIGRIVRVLRRSMLERSRLADEIVVVDGGSTDGTVEIAMAEGATVHRELDVLPE
                     EGPGSGKGEGVWKSLHVCRGDLICWIDADIRNIHPRFVYGLLGPLLLDPGIVYVKAFY
                     ERPIRERNALRATGGGRVTELLARPVLSLFWPQLAGFVQPLSGEYAGRREVLEQVPLF
                     SGYGVELGLLIDIARHAGVDAIAQVDLERRVHRNQDVQALSRMSFGILQAALARLADE
                     DRMAPDTWSTTLYQFANRLREYHIEPREITTVQRPPMATLDSYRRRRDALAAGSSA"
     gene            3978..>4725
                     /locus_tag="VEP73_00345"
     CDS             3978..>4725
                     /locus_tag="VEP73_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYT24921.1"
                     /translation="MPALRLAEAGAVLDRLDPAVLFSDVDGTLVGRGGSLLAGLDGTP
                     TLAAAEALVEAGRAGLEVVLVSGRTAAQLREASRLLGLRDAIGEVGAVLVLGGTVELQ
                     WGEAPRGVGATPAEAIERSGSLATVLDAFRGRLEPHAPWHEGRQGTALLRGQVDAVEV
                     DEVLAGAGFGWARLQDNGRLRGAYPHLGPATTHAYHLAPAGVTKARAAAAYLARRGLA
                     AGRAAAIGDAPADLELAGVVGAMFLVANGAW"
BASE COUNT          568 a         1744 c         1836 g          577 t
ORIGIN      
        1 ggcgggcgag cgggaggccg cccgggtcca cccggacggc gatcgacagg cggacggggt
       61 tcgggaagcc gggcaggagg accggcgggg tgatgcggga ggcgtcgggc acggcgccgg
      121 tgtcgagcgc ggtgccgtcg ccggccggct cgcccccgag cgggacccca gggacgtagc
      181 gcggcgcgac gacgagcggg aagcggaacg tcgcctcccc gtcggcgtac ggcagcggcc
      241 cggccatcgc gagccggacg ctcgcccgct cgccgggcat gaggttgccg acgcgcatgg
      301 tgaacacgtc cgggcgctcc tcctcggcga tcgaggcgcg gcgcccctcg gcgacggccc
      361 ggtcgtaggt ggccctggcg gcgccccgct cctcgagcac gccatcgacc acccgcccgg
      421 ccacctccat gcggaactcg gtgacggcgg cccggtcggg cagcgggaac acgtaggtgg
      481 cctcgagcgg ctcggggtgg gggttcgaga aggtctgggt cacgacgacc cgggcgagga
      541 ggccggcgac gtcggcgtcg acgtcgaccg cctcgagggg caggttgccc cgctcggtgg
      601 agagcgcgcc ggcgccggcc tcgtcgccgg cgggcacggc ccgggcgacc tcgtcgtcgg
      661 tcagcagcgg gagcgggcgg ctcgtcatgg aaggtccctt ccggtggtgg ggccgtcggg
      721 cgcgggctcg gcgagccctc gagcagcgag cgcctcgagc agcggccggg cggcggcctg
      781 cagggcgcgg acgtcgtcgt cgtccaccgg ccggtgggcg ccgatgagga gggtcgcctc
      841 ctcggagagg cggatcccct ggaggcgcgc ggccggggca ggccgcatga gcgggccggg
      901 cggccccggg gacgacggcg ccggggcggg cgaggcggcc cagaacctgc gcggggcatc
      961 ctcgcctgaa gcgcgcgagg cccgggcgcc tgaagcgcgc gaggcccggg cgcctgaagc
     1021 gcgcggcgcc tcctcgcctg gtggggcggg cgggacgcgg gcgatccgct cgaggtcggc
     1081 ctcgcccacc ccggccagct cggcctggac ggcggccagc gacaggccgc gggcctggag
     1141 ccgcttgatc gcgacgagct gcaggaggtg gcgcggcccg tacagggcgg tccggccccg
     1201 catggcggcg ggccggtcga ccaggccgag cgtggtgtag tagcggatcg cccgctggtc
     1261 gggcacgtcg cgcacccggc cgctcgcctg gccgtcgtag tcgacggcca gcgcggcggc
     1321 gaccccgcgg gtcagctcgt cgagtgtcca gaacgccgtg gtgcccatac cctcactttg
     1381 acactgtcat atgacactgt caacttccac tgaccaggtc ggggcggacc gggtcgaggg
     1441 ccgcctccag cgcgtcgatg ttcgggcgga cggtgatcgt cgcgccgtcc cggccgtcca
     1501 ggacctccct ggtcttcagg ccgtgcccgg tcaccagggc gacgatccgc tcgcccggcc
     1561 ggaaggcgcc ccgttgcgcg aggcgccgca gggtggcgat ggtcaccccg gcagcggtct
     1621 cgccgaagat gccggtggta cgggcgagca gcccgatggc gtccaccacc tcctcgtcgg
     1681 tcacggcctc gacccgcccg ccggtggagc ggacgacctc gagcgcctcc acgccgtcgg
     1741 ccgggtcgcc gatgccgagc gacttcacga tgcccgtcgg ccgcaccggc cggacgtcgt
     1801 cgcgcccctc ggcgaacgcc gccgcgaccg gcgggcagcc gtcggactgg gcgccgtgga
     1861 cgtgcaccgg ggtgccggcc accagaccgg ccgcgaccag cgtgccgaac ccctggtgga
     1921 cctttgacaa cagcgccccc gaggcgatcg ggacgaccac gtggtcgggc agggaccaac
     1981 cgagctgctc ggccacctcg agcccgacgg tcttggaccc ctccgcgtac caggggcgga
     2041 ggttgacgtt gacgaagccc cagccgaacg cctcggcggc ctgcgcggac agccggttgg
     2101 cgtggtcgta ggacccctcg acggcgacca cggtggcccc gaaggtggcc gccgaggcga
     2161 tcttgcccgc ctcgaggtcg gccgggatga ccaccacgca gtcgagcccc tgggcggccg
     2221 cggccgcggc cgtcgccccg gccaggttgc cggtcgaggc gcacgccacg gtcgagaggc
     2281 cgaactcgcg agccttggcg accgcgaccg cgaccacccg gtccttgaac gagttggtcg
     2341 ggttgcgggt gtcgtccttg caccacagct cgcccgggat cgccagcgcc tcggcgagcc
     2401 gcggcgtccg cacgagcggc gtcaggccgg gggacaggtc cccggcgccg ggacgggaag
     2461 gcaggaaggc gccgtagcgc cagatcgacg ccggcccggc ctcgatcgag gcccgggcga
     2521 gccggggatc cacggcgtcg ccgtcgtact cgacgtcgag cgggccgagg cagttgggac
     2581 aggcgtagcg gagctccggc gggtaggggg tccggcagcc gcggcaacgc agggagacgc
     2641 cgtcggcggc cggggcgggg gtgaggcggt cgtgctcggc agcggtcatg cgggtgacct
     2701 ccgtgggctc gtaggcgatc gcctcgcgcc gcggtcaccc ggaccgctcg cgcatctttc
     2761 cccgtccgtg aggcggggtc ggacgtggca ccttccccgt ccggtgtccg gtcggggagg
     2821 ttgccggggc ttcgtcgggc cggtccctcc acccctcttg atgcgtcgcc gttcttcagt
     2881 tgtcgtttcc agcttgccct ggcaggctcg gaggcgtcaa cgccgacggc ctagggtatg
     2941 ccgggcagcc gaatgacaaa cccgatctgg agaggggatg gcggagctcg acacctggga
     3001 ctggttcggc aaccggacct tccactacgg acagttcgag ccggtttcca ggctccgcgc
     3061 ggccagggag cggtcgggaa cgacggtctc gatctgcctg cccgcccgca acgaggcggc
     3121 caccatcggc cggatcgtgc gcgtcctgcg gcggtcgatg ctcgagcggt cgcgcctcgc
     3181 cgacgagatc gtcgtcgtgg acggcggctc gaccgacggc acggtcgaga tcgccatggc
     3241 cgagggcgcg accgtccacc gcgagctcga cgtgctgccc gaggaggggc ccgggtccgg
     3301 caagggggag ggcgtgtgga agagcctgca cgtctgccgc ggcgacctca tctgctggat
     3361 cgacgccgac atccgcaaca tccacccgcg cttcgtgtac ggcctgctcg ggccgctgct
     3421 cctcgacccg ggcatcgtgt acgtgaaggc cttctacgag cgcccgatcc gggagcgcaa
     3481 cgcgctgcgg gccaccggcg gcggccgggt caccgagctc ctggcgcggc ccgtgctcag
     3541 cctgttctgg ccgcagctcg ccggcttcgt ccagcccctg tcgggcgagt acgccgggcg
     3601 gcgggaggta ctcgagcagg tcccgctctt ctccggctac ggcgtcgagc tgggcctgct
     3661 catcgacatc gcgcgccacg ccggggtcga cgcgatcgcc caggtcgacc tggagcgccg
     3721 cgtccaccgc aaccaggacg tgcaggcgct ctcgcggatg agcttcggca tcctccaggc
     3781 ggccctcgcc cggctcgccg acgaggacag gatggcgccg gacacctggt cgacgacgct
     3841 gtaccagttc gccaaccgcc tgcgcgagta ccacatcgag cccagggaga tcaccaccgt
     3901 ccagcggccc cccatggcca ccctcgacag ctaccggcga cggcgtgacg cgctcgccgc
     3961 cgggtcgagc gcctgacgtg cccgcgctgc ggctcgccga ggcgggcgcc gtgctcgacc
     4021 ggctcgaccc ggcggtgctc ttctccgacg tcgacggcac cctggtgggc cggggcggct
     4081 ccctgctcgc cggcctcgac gggaccccga ccctggcggc ggccgaggcg ctcgtcgagg
     4141 ccggccgcgc cggcctcgag gtcgtgctcg tgtcgggccg gaccgccgcc cagctccggg
     4201 aggcgtccag gctcctcggc ctgcgcgacg ccatcggcga ggtcggcgcc gtgctggtcc
     4261 tcggcggcac ggtcgagctg cagtggggcg aggcgccgcg tggcgtgggc gccaccccgg
     4321 ccgaggccat cgagcgctcg ggctccctcg ccacggtcct cgacgccttc cggggcaggc
     4381 tcgagccgca tgcgccctgg cacgagggac gccagggcac cgccctgctg cgcggccagg
     4441 tggacgccgt cgaggtcgac gaggtcctgg ccggcgccgg cttcggctgg gccaggctgc
     4501 aggacaacgg gcgcctgcgg ggcgcctacc cgcatctcgg cccggccacg acccacgcct
     4561 accacctcgc gccggccggc gtgaccaagg cgcgcgcggc cgccgcgtac ctcgcccgcc
     4621 gcggcctcgc cgcgggccgg gcggcggcga tcggcgacgc cccggccgac ctcgagctgg
     4681 ccggcgtggt gggcgcgatg ttcctggtcg ccaacggcgc ctggg
//
LOCUS       DASPYY010000027         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1829865, whole genome shotgun sequence.
ACCESSION   DASPYY010000027 DASPYY010000000
VERSION     DASPYY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829865"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VEP73_00350"
     CDS             complement(<1..700)
                     /locus_tag="VEP73_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYT24922.1"
                     /translation="MGKLFGTDGVRGLANADLTPELALALGRAAVGVLRAEAEADAEA
                     DAGAGDPAGERPVVVVGRDPRASGALLESALVGGILSAGGDALLAGVLPTPAIAFLTR
                     HLRAAAGAMISASHNPMPDNGIKFFGPEGRKLPDPLEDRIEAALGAAEAYLAHLLEGL
                     PDLGGLQVVVDCANGAASSIGPEAYRRAGARVTAIAATPDGHNINHGCGSTHPERLQE
                     AVAASGASIGLAHDG"
     gene            complement(747..1145)
                     /gene="rpsI"
                     /locus_tag="VEP73_00355"
     CDS             complement(747..1145)
                     /gene="rpsI"
                     /locus_tag="VEP73_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYT24923.1"
                     /translation="MAAPTLLARGTGRRKEAVARVRLLEGTGAFKLNGRPLEDYFPNP
                     AHRRMITEPLRVTNTLERYDVIASLAGGGTSGQAGALRHGIARSLVDLDVELRPELKK
                     RGFLTRDAREVERKKYGLKKARKAPQYSKR"
     gene            complement(1171..1641)
                     /gene="rplM"
                     /locus_tag="VEP73_00360"
     CDS             complement(1171..1641)
                     /gene="rplM"
                     /locus_tag="VEP73_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYT24924.1"
                     /translation="MPLASQRTFSPRPQDITREWFVVDADGQTLGRLAAQVAAILRGK
                     HKPIFAPHMDTGDHVIIVNAAKIRLTGNKLQQKNVYRHSGYPGGLKTTPYTVLLAQRP
                     SRAMEKAVRGMLPHNRIGRQMLKKLKVYDGPTHPHQAQAPKPLDLGPDSGAKQS"
     gene            complement(1744..2517)
                     /gene="truA"
                     /locus_tag="VEP73_00365"
     CDS             complement(1744..2517)
                     /gene="truA"
                     /locus_tag="VEP73_00365"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYT24925.1"
                     /translation="MRRLMLVVAYDGRPFSGFARQKDRRTVQGDLERLLAQLAKAPVT
                     TVGAGRTDAGVHARGQVVHADIPDEARPLDPERVRLALNGALGPAIVVREAAWAPDGF
                     DARLSARRRTYVYRVDDSTAPDPLTSGFVLHYRRPLDLERMRLAAGALLGEHDFAAFC
                     RRAPGGTTMRRLRSLSIRRRGGLVEFKLVADAFCWQMVRSLVGYLLAVGDGRRDPAEA
                     PAVLASGDRAAAGQLAPPHALVLETVSYPRKLTEIAGDR"
     gene            complement(2527..>2858)
                     /locus_tag="VEP73_00370"
     CDS             complement(2527..>2858)
                     /locus_tag="VEP73_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24926.1"
                     /translation="DDEQQEGGRRRFGRGRREATAAPEGSNRATRRAAARGKGPAPAP
                     VEAEAAEAEAEAEDGEERPDVPTDAEIEAGVAAPVDAEDEDEAAEAEADGEAAGDAEA
                     GEEKGKA"
BASE COUNT          346 a         1001 c         1091 g          420 t
ORIGIN      
        1 cgccgtcgtg agccaagccg atgctcgcgc cggaggcggc gaccgcctcc tggaggcgtt
       61 ccggatgggt ggagccacag ccgtggttga tgttgtgccc gtccggggtg gctgcgatcg
      121 cggtgacgcg cgccccggca cggcggtagg cctccgggcc gatgctggag gccgcgccgt
      181 tggcgcagtc gaccaccacc tggaggccgc ccaggtccgg caggccttcc agcaggtggg
      241 cgaggtaggc ctcggcggcg ccgagggcgg cctcgatgcg gtcctccagc gggtcgggga
      301 gcttgcggcc ctccggcccg aagaacttga tgccgttgtc cggcatgggg ttgtgcgacg
      361 ccgagatcat ggcacccgcc gccgcccgca ggtgcctggt aagaaacgcg atcgccggcg
      421 tcggcaggac cccggcgagc agcgcgtcgc cgccagcgct gaggatcccc cccacgagcg
      481 cgctctccag cagcgccccg gaggcgcgcg gatcacgccc gacgaccacc acggggcgct
      541 cccccgccgg gtccccggcc ccagcgtcgg cttcggcgtc agcttcggcc tcggcgcgca
      601 gcacaccgac ggcggcgcgg ccgagggcca gcgccagctc tggcgtcagg tcggcgttgg
      661 ccagcccgcg cacgccgtca gtcccgaaca gcttgcccaa gttcacgacc ccccgcgtga
      721 tggcccggtg caggtgcagg gataggtcag cgcttggagt actgcggcgc cttgcgggcc
      781 ttcttgaggc cgtacttctt gcgctcgacc tcgcgggcgt cacgggtgag gaagccgcgc
      841 ttcttcagct cggggcgcag ctcgacgtcg aggtcgacca gggagcgggc gatgccgtgg
      901 cgcagcgcgc ctgcctggcc ggaggtgcct cccccggcca gggaggcgat cacgtcgtag
      961 cgctccaggg tgttggtgac ccgcagcggc tcggtgatca tccggcggtg ggccgggttg
     1021 gggaagtagt cctcgagcgg ccggccgttc agcttgaagg cgccggtccc ctcgagcagc
     1081 cgcacccggg ccacggcctc cttgcggcgg ccggtgcccc gggcgagcag tgtgggtgct
     1141 gccaaggtga gtttcctcct gtccctgttc ctagctctgc ttggcgcccg agtccgggcc
     1201 gaggtccagc ggcttgggcg cctgggcctg gtgcgggtgg gtcgggccgt cgtagacctt
     1261 gagtttcttg agcatctgcc gcccgatgcg gttgtggggc agcatgcccc ggaccgcctt
     1321 ctccatcgcg cgcgagggcc gctgggccag cagcacggtg tagggggtgg tcttgaggcc
     1381 gccggggtac ccagagtggc ggtagacgtt cttctgctgc agcttgttgc cggtgagccg
     1441 gatcttcgcc gcgttgacga tgatcacgtg gtcgccggtg tccatgtgcg gcgcgaagat
     1501 cggcttgtgc ttgccgcgca ggatcgccgc gacctgggcg gcgagacgcc cgagggtttg
     1561 gccgtccgcg tcgacgacga accactcgcg ggtgatgtcc tgcgggcgcg gcgagaatgt
     1621 gcgctggctt gcgagaggca tgcgggcaac tccatgaacg gacaagaatc gtgggttggg
     1681 cggcatttac gacgccaccg ttcctcaagt cgacagcttg tgtccacttg aggaaacacg
     1741 aggctaccgg tcacctgcta tttcggtcaa ctttcgtgga taggagaccg tctccaggac
     1801 gagggcgtgc gggggtgcga gctggccggc agcggcgcgg tcgccggagg cgaggacagc
     1861 gggggcctcg gcggggtcgc ggcggccgtc gccgacggcg agcaggtagc cgacgaggct
     1921 gcgcaccatc tgccagcaga aggcgtcggc gaccagcttg aactccacca agccaccgcg
     1981 gcgccggatc gagagggagc gcaggcgccg catggtggtg ccgcctggag cgcggcggca
     2041 gaaggccgcg aagtcgtgct cccccagcag cgcacccgcc gccaggcgca tgcgctccag
     2101 gtcgagcggg cgccggtagt ggagcacgaa cccgctggtg agcggatccg gggcggtgga
     2161 gtcgtcgacc cggtagacgt aggtgcggcg ccgcgcggac aggcgggcgt cgaagccgtc
     2221 gggggcccag gccgcctccc ggaccacgat cgccggcccg agcgcgccgt tgagggcgag
     2281 gcgcacccgc tcggggtcga gcgggcgggc ctcgtcaggg atgtcggcgt ggaccacctg
     2341 gccgcgggcg tgcacgcccg catcggtccg cccggcgcca accgtggtca ccggcgcctt
     2401 ggcgagctgc gcgaggagcc gttccagatc accctggacg gtgcgccggt ccttctgccg
     2461 ggcgaacccg gagaacggcc ggccgtcgta ggcgaccacc agcatcagcc ggcgcatctc
     2521 tcctggctac gccttcccct tctcctcgcc ggcctcggcg tcgcccgcag cctcaccgtc
     2581 ggcctccgcc tcggcggcct cgtcttcgtc ctcggcgtcg accggggcgg cgacgcccgc
     2641 ctcgatctcg gcgtcggtcg ggacgtccgg gcgctcctcg ccgtcctccg cctccgcctc
     2701 ggcctcagcg gcctcggcct cgacgggcgc cggggcgggg cccttgccgc gtgcggcggc
     2761 gcggcgggtg gcgcggttgg agccctcggg cgcggcggtg gcctcgcgac ggccacggcc
     2821 gaagcggcgg cgaccaccct cctgctgctc gtcgtcca
//
LOCUS       DASPYY010000028         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2672889, whole genome shotgun sequence.
ACCESSION   DASPYY010000028 DASPYY010000000
VERSION     DASPYY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..610)
                     /locus_tag="VEP73_00375"
     CDS             complement(212..610)
                     /locus_tag="VEP73_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HYT24927.1"
                     /translation="MTIWLLGLAVMLLFIGGLSLDLWRAFTERQALANAVDAAAIAGA
                     SGIDELRFRASGTLALSPSRAEQLAWDNLRAQSVGHRLSAARVDATPARVEVVASTTV
                     DFTLLRVLLPRERPFTVTAHAVSVPVESGA"
     gene            complement(655..1104)
                     /locus_tag="VEP73_00380"
     CDS             complement(655..1104)
                     /locus_tag="VEP73_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24928.1"
                     /translation="MTPATRARTRRRRRARSGGAGYAAVEFVLGICLVVLPVALLVLS
                     LPIWSERQSMAQRAAEESARQVVLAPSWQDGVVEGRRVVAEVARNYGLPAGALRVRFD
                     GALQRGATVTSRVTVPMPALAIPLVGRAAAWHWTAVHVEHVDSYRSL"
     gene            complement(1101..1490)
                     /locus_tag="VEP73_00385"
     CDS             complement(1101..1490)
                     /locus_tag="VEP73_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24929.1"
                     /translation="MPARTQGTRAGGFVTAQFVVVVALSLVFFVFLCNAVVFMYGRGV
                     VRAALEEGVRAGSPAGSSTAACRRAADDAIRSLLGGRMGAQVRISCALAAGEVRATAD
                     VRFQGWLPGVPDWRFRNDASAVKERLP"
     gene            complement(1540..>1758)
                     /locus_tag="VEP73_00390"
     CDS             complement(1540..>1758)
                     /locus_tag="VEP73_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24930.1"
                     /translation="GRMLVRCYLALLVLGGRALGAVHGRPRGEAGFSTAEGLAGAALA
                     VIALVAIWGALKALGMDVVDWMRSQIIK"
BASE COUNT          243 a          672 c          657 g          186 t
ORIGIN      
        1 gccggccgtc gtcggcgggg tcgcgcgggt ggtgggcggg gccgtggggg cggcggccgt
       61 gccgggggag ggctcgccgt cgccgccccg gttcagcagc acgagggcca gggcgatggc
      121 gagcagcacc gggatggcga ggccgaccgc gaggcgcgac cggctcatgc tcgggcctgc
      181 ctcatgctcc ggccggctca tgtcccccgc ctcatgctcc ggactccacg ggcaccgaga
      241 cggcgtgggc ggtgaccgtg aacggccgct cgcggggaag caggaccctg aggagggtga
      301 agtcgacggt ggtgctcgcg accacctcga cccgggccgg ggtcgcgtcc accctggcgg
      361 ccgacaggcg gtggccgacc gactgcgcgc gcaggttgtc ccaggcgagc tgctcggccc
      421 ttgacgggga cagggcgagg gtgcccgagg cgcggaagcg gagctcgtcg atgccggacg
      481 cgcccgcgat ggcggctgcg tcgacggcgt tcgcgagcgc ctggcgctcg gtgaacgcgc
      541 gccacaggtc gaggctcagg ccgccgatga acaggagcat caccgcgagc ccgagcagcc
      601 agatcgtcac ggaccctcgc tcccggtcaa tcgccctggc catggcacac gccctcaaag
      661 gctccggtaa ctgtccacgt gctcgacgtg gacggcggtc cagtgccagg cggcggcccg
      721 gccgacgagc gggatggcga gggcgggcat ggggacggtg acccggctcg tcacggtcgc
      781 cccccgctgc agcgcgccgt cgaagcggac ccgcagcgca ccggcgggca ggccgtagtt
      841 gcgcgccacc tcggccacga cccggcggcc ctcgacgacc ccgtcctgcc agctcggggc
      901 gaggacgacc tggcgggcgc tctcctcggc cgcccgctgg gccatcgact gccgctccga
      961 ccagatcggc agggacagca ccagcagcgc gacgggcagg acgacgaggc agatgccgag
     1021 gacgaactcg accgcggcgt acccggcgcc gccggagcgg gcgcggcgac ggcgccgggt
     1081 gcgcgctcgg gtcgccggtg tcaaggcagc cgctccttca ccgcgctggc gtcgttcctg
     1141 aaccgccagt cgggcacgcc gggaagccag ccctggaacc ggacgtccgc ggtcgcccgc
     1201 acctcgccgg cggccagcgc gcaggagatg cggacctgcg cgcccatcct gcccccgagc
     1261 aggctccgga tggcgtcgtc ggccgcccgc cggcacgcgg ccgtcgagct gccggccggg
     1321 ctgcccgccc gcacgccctc ctccagggcg gcccggacca cgccccgccc gtacatgaac
     1381 acgacggcgt tgcagaggaa cacgaagaac accagggaca gggccaccac gacgacgaac
     1441 tgggcggtca cgaagccgcc cgctcgggtc ccctgcgtcc gcgctggcat cgctcacctt
     1501 ccgacgtggc cctggtcccg cggccgtggt ccctcggcgc tacttgatga tctgcgaccg
     1561 catccagtcg acgacgtcca tcccgagcgc cttcagcgcc ccccagatgg cgaccagcgc
     1621 gatcacggcg agggccgcgc cggccaggcc ctcggcggtc gagaaccccg cctcgccgcg
     1681 cggccggcca tggacggcgc cgagcgcgcg ccccccgagc acgagcagcg ccaggtagca
     1741 gcgaacgagc atccttcc
//
LOCUS       DASPYY010000029         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2251597, whole genome shotgun sequence.
ACCESSION   DASPYY010000029 DASPYY010000000
VERSION     DASPYY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251597"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /gene="tnpB"
                     /locus_tag="VEP73_00395"
     CDS             complement(<1..1148)
                     /gene="tnpB"
                     /locus_tag="VEP73_00395"
                     /inference="COORDINATES: protein motif:HMM:NF038280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family element RNA-guided endonuclease
                     TnpB"
                     /protein_id="HYT24931.1"
                     /translation="MGARQKFEIPEGWVARGFRFEVEATTAEQQELIHQHFGARRFAH
                     NWALAQVRANLDARAVDPAVPALGWSLPALRKAWNQAKQQVAPWWRECSKEAYSSGIA
                     DLVAALHNWSDSKAGRRAGRRVGFPRFKARHRDRGRVRFTTGAMRLEPDRRHLTLPVI
                     GRLRSKENTRRLQRLVATGRARILSMTLSEHGGRLFVSVATIVCHTPRTPAEPDARCG
                     IDLGIGHEWAVIAHDDDTIQRVAHPAPWAQTHMKRCRVARQRSRRIVGSRGHRQVSAK
                     LAALDRRAANLRTDQVHLLTTILARRYGTIVVEDLDIAAMGRAMGRRAFRRSVARAGI
                     GKVRPTLAYKTAQEGGRLLLAERWFASSKTHHGCGGYLADLKLSQRAWA"
     gene            complement(1148..1761)
                     /locus_tag="VEP73_00400"
                     /pseudo
     CDS             complement(1148..1761)
                     /locus_tag="VEP73_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(1780..>2744)
                     /locus_tag="VEP73_00405"
     CDS             complement(1780..>2744)
                     /locus_tag="VEP73_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24932.1"
                     /translation="PLVALGGGAVVKIDRNGTVTSLTPGGFQAGGAARRGDDVLACGR
                     RPLPPGERSAARAAGRDQTTAAPALLLPGTTGRATVLALGCPVSWAAAADVVAGAGGP
                     QVAYRRTTRGASVLAGPHGGPLGLTVSLRAVTSFAVAVASAGAMYLRAAGESVLSDRL
                     GSAPPSASGLGVAQRVAGKRDLRGLDATLRAAMPALPPLEVPVTQLETAEPTALGRPG
                     STQPARTRQTARLGQPVRPLPAPRRRARPLPRADLAGRPRRGGDLGAGSRQAAHGRRG
                     RARRRGPRRERRRTAGEGDHGLPAHPGRPLVVGVANCWPMGRRT"
BASE COUNT          350 a         1016 c         1026 g          352 t
ORIGIN      
        1 gcccatgccc gctggctgag cttgaggtcg gccaggtagc cgccgcagcc gtggtgggtc
       61 ttggacgacg cgaaccaccg ctcggccagc agcagccgac cgccctcctg ggcagtcttg
      121 taggccaggg tcgggcgcac cttgccgatc ccggcccggg cgacgctgcg ccgaaacgct
      181 cgccggccca tcgcgcggcc catcgcggcg atgtcgagat cctcgacgac gatggtgccg
      241 tagcggcgcg ccaggatggt ggtaaggagg tggacctggt cggtgcggag gttggccgct
      301 cgccggtcga gggccgccag cttggcgctc acctggcggt ggcctcggga gccgacgatg
      361 cggcgggacc gctgccgggc gacccggcag cgcttcatgt gggtctgggc ccacggggcg
      421 ggatgggcga cccgctggat ggtgtcgtcg tcgtgggcga tgaccgccca ctcgtgcccg
      481 atccccaggt cgatcccgca acgcgcgtcg ggctcggctg gggtgcgggg ggtgtgacag
      541 acgatggtgg cgaccgacac aaacagccgc ccgccatgct ccgagagggt catcgacagg
      601 atccgcgccc ggcccgtggc gaccaggcgc tgcagtcgcc gggtgttctc cttggagcgg
      661 agccggccga tgaccggcag ggtgaggtgg cggcggtcgg gctccaggcg catcgccccg
      721 gtggtgaagc ggacccggcc acgatcccgg tgacgcgcct tgaagcgggg gaacccgacc
      781 cgcctgcccg cccgccggcc tgccttgctg tccgaccagt tgtgcagggc ggcgaccagg
      841 tcggcgatcc ccgacgagta ggcctccttg gagcactcgc gccaccacgg cgcgacctgc
      901 tgcttggcct ggttccacgc cttgcggagc gcgggcaggc tccacccaag cgccggcacc
      961 gcagggtcga ccgcccgcgc gtcgaggttc gctctgacct gggccagtgc ccaattgtgg
     1021 gcgaaccgtc gggcgccgaa atgctgatgg atcagctcct gctgctcggc ggtggtcgcc
     1081 tccacctcga accgaaagcc gcgggcaacc cagccctcgg ggatctcgaa cttctggcga
     1141 gcgcccatca gccgtcctgg ctgccggtgg gatcggggcg ggcaggccgc ttggcgcagg
     1201 tcagcgcccg catggcgcgg ttgcgggcgc cacggcggcc ataccgccgg gcgcagaagc
     1261 cggtgagcac atcgagcatg tcgcgcacga ggtcgtcatc ggtctcgccg gggtcgacca
     1321 ccaccagcct gcggccctgc gcggacagcg ccgcctccaa atgctccacc ccgaagcggg
     1381 ccagccggtc gcggtgctcc acgacgatcg tggtcgcctg cggctgggcg agcagccggc
     1441 gcagcctcgg ccgcttgccg ttcaggccgg agccgacctc acagaccacc tcatcgaccg
     1501 ctaggccctg ccgggtggcc cactgggtca cccgggcgac ctgccggtcg agatcaacac
     1561 gctggtcgtg ggaggacacc cgtgcgtaga cgaccaaacg gccggcacgg cttggctcga
     1621 cgtccaccag gatcgttccc gtcggggtct gcgagcgggc acggggagct tgccggcccg
     1681 ccaccatcgc cacgctgtcg tgtagtggat gccctcgcgt tgcgcccact cggaagcttc
     1741 acgccgccga caatagcaca tacgttcgcc tacgttcgcc tacgttcgcc tacccatcgg
     1801 ccaacagttc gcgaccccca ccaccagggg tcgtccgggg tgggccggta gaccgtgatc
     1861 accttcgcct gccgtgcgac gccgttcgcg ccgagggccg cggcgtcgag cgcgtccccg
     1921 acgcccatgt gcagcttggc ggctgcccgc tccgagatcg ccgcctcggc gggggcgtcc
     1981 agcgaggtcg gcccggggca gcggccgcgc acgacgacga ggtgctggca gaggccggac
     2041 cggctgaccc agcctcgctg tctgcctcgt cctggccggc tgggtcgagc cgggccggcc
     2101 gagcgcggtc ggctctgccg tctcgagctg ggtcaccggc acctcgagcg ggggcagggc
     2161 cggcatcgcg gcccggagcg tcgcgtcgag cccgcgcagg tcgcgctttc cggcgacccg
     2221 ctgcgcgacc ccgagcccgg acgcggacgg cggcgccgag ccgagccggt cggagagcac
     2281 cgactcgccc gccgcccgca ggtacatcgc gccggcgctg gccaccgcga cggcgaagga
     2341 cgtgaccgcg cggagggaga cggtgagccc gagcggcccg ccgtggggac cggcgagcac
     2401 cgaggcgccc cgcgtggtgc ggcggtacgc cacctggggg ccaccggccc ccgcgaccac
     2461 gtcggcggcg gctgcccagc tcaccgggca gccgagcgcc agcacggtgg ccctgcccgt
     2521 cgtgcccggc aggagcaggg ccggcgcggc ggtggtctgg tccctgccgg ccgccctcgc
     2581 ggcggagcgc tcgccggggg gaagcgggcg ccgcccgcag gcgagcacat cgtcccccct
     2641 tcgggcggcg ccaccggcct ggaagccgcc gggggtcagc gaggtgaccg ttccgttccg
     2701 gtcgatcttc acgaccgcgc cgccgccgag cgcgacgagc ggcg
//
LOCUS       DASPYY010000030         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2251638, whole genome shotgun sequence.
ACCESSION   DASPYY010000030 DASPYY010000000
VERSION     DASPYY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251638"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2867)
                     /gene="ileS"
                     /locus_tag="VEP73_00410"
     CDS             complement(<1..2867)
                     /gene="ileS"
                     /locus_tag="VEP73_00410"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYT24933.1"
                     /translation="MLALWRARKVFERSVDQRAGADPFVFYEGPPTANGRPGVHHVEA
                     RVFKDLFPRFKTMRGYRVDRKGGWDCHGLPVELEVERELGLAHKQEIEAFGVEAFNAR
                     CRESVERYVGVFEDLTERIGFWIDTSQAYRTMDQSYVESVWWALAELYRRDLLFEDYK
                     VLPYCPRCGTGLSDAELGLDAYQEVDDPSVYVMFAATSGRLAAERAALLVWTTTPWTL
                     VSNAAVAAGPEIGYVLVEATGPGGGTYRLVLAEARMAEVVGEDARVLRRVPFDELLGT
                     TYQRPFELVEVAPEEAAGAWRVVAADYVTTTAGTGLVHTAPAYGTEDLEVGRREGLPV
                     IHPVDREGRFAEGTGELSGLHVKAADPLIVELLRAAGTLWREETYRHDYPHCWRCKTP
                     LLYYALTSWYARTTAVADRMLAENAEVGWQPEHIRDGRFGDWLANNVDWALSRARYWG
                     TPLPLWRCESGHVTAVESLADLSARAGRDLSGLDPHRPYVDAVTFACPECGREARRVP
                     DVIDAWFDSGSMPFAQWGYPHTGTGEFERAFPADFISEAIDQTRGWFYSLLAVNTLLF
                     DRSPYRNVVCLGHIVDRDGRKMSKSLGNVLDPFDLLDRYGADPLRWFMLATGSPWVSR
                     RLSPEALEEVTRSFFLTLWNTYAFLTLYARLAGWVPSGGAESGPPDPPATPTAALDRW
                     ALARVRETVAGVTADLDAYDATVAARRLSSLVDDLSNWYVRLSRRRFWRGSGKDASAA
                     FRTLWTSLRTTALLLAPYAPFVSEALWQGLVVAVDPDASDSVHLESWPRDAPVEGDAE
                     LLAAMGEVRRLVGLGRQARTEARTKVRQPLARALVTVRPGLREAVAPLLDLVAAELNV
                     KQVGFAEGDAGLVAFRLSPNFRALGPRFGRQAPAVAAAVRTADAGALASRLQAGEGVE
                     VDVPGVGPVRLGPGEVGVAEEPVVGWRVVRDGATSVALDLEV"
     gene            3451..>3763
                     /locus_tag="VEP73_00415"
     CDS             3451..>3763
                     /locus_tag="VEP73_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYT24934.1"
                     /translation="MELLRRRRADATRPGARTDPFKVGLAVEGGGMRGVVSGGMLNAV
                     EDLGLAGAVDAVYGCSSGAVNAAYFLMGNSWYWLSIYYDDLPGRSFLDFRRAARGRPV
                     LA"
BASE COUNT          489 a         1400 c         1386 g          488 t
ORIGIN      
        1 acctcgaggt cgagggcgac gctggtggca ccgtcgcgga cgacccgcca gccgaccacc
       61 ggctcctcgg ccacgccgac ctccccgggg ccgagccgga ccggcccgac gccgggcacg
      121 tcgacctcga ccccctcacc cgcctggagc cgggaggcga gcgcgccggc gtcggccgtg
      181 cgcacggccg cggcgaccgc cggcgcctgc ctgccgaacc gcgggccgag cgcccggaag
      241 ttggggctga gccggaacgc gaccagcccg gcgtcgccct cggcgaaccc gacctgcttc
      301 acgttcagct cggcggcgac gaggtcgagc agcggtgcca ccgcctcgcg caggccgggc
      361 cgcacggtca cgagcgcgcg ggcgagcggc tggcgcacct tcgtcctcgc ctcggtgcgg
      421 gcctggcgcc cgagccccac gagccggcgc acctcgccca tggcggcgag cagctccgcg
      481 tcgccctcga ccggcgcgtc ccgcggccag ctctcgaggt ggaccgagtc gctcgcgtca
      541 gggtcgaccg cgaccacgag cccctgccag agggcctccg aaacgaacgg tgcgtacggg
      601 gcgagcagca gcgcggtcgt gcgcaggctc gtccacaggg tccggaaggc ggccgaggcg
      661 tccttgccgg agccgcgcca gaagcggcgc cgcgacaggc gcacgtacca gttggagagg
      721 tcgtcgacga gcgacgagag ccgccgggcg gccacggtgg cgtcgtaggc gtccaggtcg
      781 gcggtgacgc cggccacggt ctcccgcacc cgggcgagcg cccagcggtc gagcgccgcc
      841 gtgggggtcg cgggggggtc cggggggccg ctttccgccc ctccggaggg aacccacccc
      901 gcgagccggg cgtagagcgt gaggaacgcg taggtgttcc acagggtcag gaagaaggac
      961 cgggtgacct cctcgagcgc ctcgggcgag agccgccgcg agacccaggg cgagccggtc
     1021 gcgagcatga accagcgcag cgggtcggcc ccgtagcggt cgagcaggtc gaacgggtcg
     1081 agcacgttgc cgaggctctt cgacatcttg cgcccgtccc ggtccacgat gtggccgagg
     1141 cagaccacgt tccggtacgg cgaccggtcg aacagcaggg tgttcacggc gagcagcgag
     1201 tagaaccagc cgcgggtctg gtcgatcgcc tccgagatga agtcggccgg gaacgcccgc
     1261 tcgaactcgc cggtcccggt gtgcgggtag ccccactggg cgaagggcat ggacccggag
     1321 tcgaaccagg cgtcgatgac gtccgggacg cgccgcgcct cccgcccgca ctcggggcag
     1381 gcgaacgtca ccgcgtcgac gtagggccgg tgcgggtcga gcccggacag gtccctgccg
     1441 gccctggccg acaggtcggc gaggctctcg accgccgtga cgtggccgga ttcgcagcgc
     1501 cagagcggca gcggcgtccc ccagtagcgg gctcgcgaga gcgcccagtc cacgttgttg
     1561 gccagccagt ccccgaagcg gccgtcgcgg atgtgctccg gctgccagcc gacctcggcg
     1621 ttctccgcca gcatccggtc ggcgaccgcc gtggtccgcg cgtaccagga cgtgagggcg
     1681 tagtagagca ggggggtctt gcagcgccag cagtgcgggt agtcgtggcg gtaggtctcc
     1741 tcccgccaga gcgtcccggc cgccctgagc agctcgacga tgagcgggtc ggccgccttc
     1801 acgtgcagcc cggagagctc cccggtgccc tccgcgaacc ggccctcccg gtcgaccggg
     1861 tggatgaccg gcagcccctc gcgccggccc acctccaggt cctcggtccc gtacgcgggc
     1921 gcggtgtgga cgagcccggt gccggcggtg gtggtgacgt agtcggcggc caccacgcgc
     1981 caggcgcccg ccgcctcctc gggcgccacc tcgaccagct cgaacggccg ctggtaggtg
     2041 gtcccgagca gctcgtcgaa cggcacccgg cgaagcaccc tggcgtcctc gccgaccacc
     2101 tccgccatcc gcgcctcggc aagcaccagc cggtaggtgc cgccgcccgg tccggtcgcc
     2161 tccacgagca cgtagccgat ctccggcccc gccgccacgg ccgcgttcga gacgagcgtc
     2221 cacggcgtcg tcgtccacac gagcagggcg gcccgctccg cggccaggcg gccggaggtg
     2281 gcggcgaaca tgacgtagac cgaggggtcg tcgacctcct ggtaggcgtc gaggccgagc
     2341 tcggcgtccg acagcccggt gccgcagcgc gggcagtacg ggagcacctt gtagtcctcg
     2401 aagaggaggt ccctccggta cagctcggcg agcgcccacc agaccgactc gacataggac
     2461 tggtccatcg tccggtacgc ctggctcgtg tcgatccaga agccgatgcg ctcggtgagg
     2521 tcctcgaaca cgccgacgta gcgctcgacc gactcccggc acctggcgtt gaacgcctcg
     2581 accccgaagg cctcgatctc ctgcttgtgg gcgaggccga gctcccgctc gacctcgagc
     2641 tcgaccggca gcccgtggca gtcccagccg cccttgcggt cgacccggta gccacgcatc
     2701 gtcttgaacc tcggaaacag gtccttgaag acgcgggcct cgacgtggtg cacgccgggc
     2761 cggccgttgg cggtcggggg gccctcgtag aagacgaagg ggtcggcgcc ggcccgctgg
     2821 tcgacggagc gctcgaacac cttccgcgcg cgccacagcg cgagcacccg ccggtcgagg
     2881 gcgggcaggt cgaggttggg gtcgacgtcg gggaactgcc catcggtggt ctcctccgcg
     2941 gtcgcgttcg tcggttcctc gcgaagggac gaggcccgag gccccgcggt accacccctc
     3001 ttgccggcgt ggtcctccca cggctcgcgg aagggcgccg gcccctcacg accacccgaa
     3061 gatggcctcc cgctgtcacg ggcggacccg gctgcgtcta cttggcggtc ccggtcccag
     3121 cgtcaccggg cgcccgccgt tcgggcagcg gctcggggag gatgttcacg ggtgccgtgc
     3181 caccgggctt ccaccgtccc cggctcgctc gcggccgtgc cgcccgctac tcgttcccgt
     3241 catcgccttt cccgaagtgt accgtccccg ggcaacccgg ttccggccgt tccctccagg
     3301 tgggtacggt cgtgttgccg gtccggccgc cgcctgccag gcttgtgacg tgagtcgtgg
     3361 cgacccgcga gggacctgcc gccgagtgag gcggtcttga cagcgttgcc tccttccccc
     3421 tcggcgcctt cggcgccaga ccatcccgtg gtggagctgc tgcggcggcg gcgggcggac
     3481 gccacccgcc cgggcgcgcg aaccgacccg ttcaaggtcg gcctggccgt ggagggcggc
     3541 gggatgcgcg gggtggtctc gggcgggatg ctcaacgccg tcgaggacct ggggctggcg
     3601 ggcgccgtcg acgcggtcta cggctgctcc tcgggggccg tgaacgccgc ctacttcctg
     3661 atgggcaaca gctggtactg gctgtcgatc tactacgacg acctgcctgg ccggtccttc
     3721 ctggacttcc gccgggccgc gcgggggcgg ccggtgctgg cgg
//
LOCUS       DASPYY010000031         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1689882, whole genome shotgun sequence.
ACCESSION   DASPYY010000031 DASPYY010000000
VERSION     DASPYY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689882"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VEP73_00420"
     CDS             <1..1051
                     /locus_tag="VEP73_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HYT24935.1"
                     /translation="YHVLRELEARELPAVRALGLVGQPDSGNAILITRYLEHSWQYRR
                     VFQRLPWGLTRHRERLLDAMAGLVVDLHRSGVFWGDCSLGNTLFARDGQLLQAFLVDA
                     ETSEIHPALSDGQRALDLGILVENVAGDLVDLSFRLGRAEQVDEDLAAAESVATRYEW
                     LWEELSREETFGPDERYKVEKRVRRLNELGFAVDELAMEPATPDRSTLRLKVCVANRR
                     FHTNQLRRLAGLEAGEGQATILLNDLRAYQSRLEQERPGSAPDDVAGLRWLTEVFRPQ
                     SARAAAALGPGTDSVQAYCDLLEVRWLLSEEAGRDVGDKVVLKALSSRQTPSGAAAEL
                     VVAESPTGTFPPVRP"
     gene            complement(1068..1673)
                     /locus_tag="VEP73_00425"
     CDS             complement(1068..1673)
                     /locus_tag="VEP73_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYT24936.1"
                     /translation="MMARTGRRPGGGGTRAAILDAARRAFAERGYDHATIRAVAEAAR
                     VDPALVHHYFGSKERLFVAAMDLPVDPVGIVAMLLDGDRERIGERIVGTFVSVWDQTA
                     NRNPLIALIRSAVSSDRAAVMLREFILRELFGRITDRLGMPDSRLRASLVASQLMGLA
                     VARYIVGLDPIASASPAELVATIGPTIQRYVTGDIRAEAGR"
     gene            complement(1670..>2023)
                     /locus_tag="VEP73_00430"
     CDS             complement(1670..>2023)
                     /locus_tag="VEP73_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT24937.1"
                     /translation="GAGALVTTHSMGEVEQCDRLVVMAGGRVAAEGTMAAIMGDARTV
                     RVAAPGWEDAFVALEDAGLPVALVGRSLRVPGADRRRVAEALGAAGVEADLRLVPATF
                     EETFVALATGGRAAA"
BASE COUNT          266 a          751 c          760 g          246 t
ORIGIN      
        1 gtaccacgtc ctgcgcgagc tggaggcgcg cgagctgccc gccgtgcgcg ccctcgggct
       61 ggtggggcag cccgactccg gcaacgccat cctcatcacc cgctacctcg agcactcctg
      121 gcagtaccgg cgcgtgttcc agcgcctgcc ctgggggctg acccggcacc gcgagcggct
      181 cctcgacgcg atggcgggcc tggtcgtcga cctgcaccgc agcggggtgt tctggggcga
      241 ctgctcgctc ggcaacaccc tgttcgcgcg ggacgggcag ctcctgcagg cgttcctggt
      301 cgacgccgag acgagcgaga tccacccggc cctctcggac gggcagcgtg ccctcgacct
      361 cggcatcctg gtcgagaacg tcgccgggga cctcgtcgac ctgagcttcc ggctcgggcg
      421 ggccgagcag gtcgacgagg acctcgccgc ggccgagagc gtggccaccc gctacgagtg
      481 gctctgggag gagctgtcgc gcgaggagac gttcgggccg gacgagcggt acaaggtcga
      541 gaagcgggtg cgccggctca acgagctggg cttcgccgtc gacgagctcg ccatggagcc
      601 ggcgacgcca gaccggagca cgctgcggct caaggtctgc gtggccaacc ggcggttcca
      661 cacgaaccag ctgcgcaggc tggccgggct cgaggcgggg gaggggcagg ccaccatcct
      721 gctcaacgac ctgcgcgcct accagagccg gctcgagcag gagcgacccg ggtcggcacc
      781 cgacgacgtc gccggcctgc gctggctgac cgaggtcttc cggccccagt ccgccagggc
      841 ggccgccgcg ctcgggccgg gcaccgactc cgtgcaggcg tactgcgacc tgctcgaggt
      901 ccgctggctg ctcagcgagg aggccggacg cgacgtgggc gacaaggtcg tcctgaaggc
      961 gctctcgtcc aggcagacgc cgtccggggc ggcggccgag ctcgtggtgg cggagtcccc
     1021 gaccggcacc ttccccccgg tgcgaccctg acgccgccgc cgggccgtca gcggccggct
     1081 tcggctctga tgtcgccggt cacgtagcgc tggatcgtcg ggccgatggt ggcgaccagc
     1141 tcggccggcg aggccgaggc gatcgggtcg agcccgacga tgtagcgggc caccgcgaga
     1201 cccatgagct gagaggcgac gaggctcgcg cgcagccggc tgtcgggcat ccccaggcgg
     1261 tcggtgatgc ggccgaacag ctcgcggagg atgaactcgc gcagcatgac ggctgccctg
     1321 tcgctcgaca ccgccgagcg gatgagcgcg atgagcgggt tgcggttggc ggtctggtcc
     1381 cacacggaca cgaaggtgcc gacgatccgc tcgccgatcc gctcccggtc gccgtcgagc
     1441 agcatggcga cgatcccgac cgggtcgacg ggcaggtcca tggcggccac gaacagccgc
     1501 tccttgctgc cgaagtagtg gtgtaccagg gccgggtcga ccctggcggc ctcggcgacc
     1561 gcgcggatgg tggcatggtc gtagccccgc tcggcgaagg cccgccgggc ggcgtcgagg
     1621 atggcggcac gcgtgccgcc gccgcccggc cgccgcccgg tccgcgccat caggcggccg
     1681 cccggccgcc ggtggccagc gccacgaacg tctcctcgaa cgtggcgggc acgagccgga
     1741 ggtcggcctc gacgcccgcg gcgccgagcg cctcggctac ccgccgccgg tcggcgccgg
     1801 ggacccgcag ggaccggccg accagcgcga cgggcagccc ggcgtcctcg agcgccacga
     1861 acgcgtcctc ccagcccggc gcggccaccc gcacggtcct ggcgtcgccc atgatggcgg
     1921 ccatggtccc ctcggcggcg acccggccgc cggccatgac caccagccgg tcgcactgct
     1981 cgacctcgcc catggagtgg gtggtgacga gcgcgccggc gcc
//
LOCUS       DASPYY010000032         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2251891, whole genome shotgun sequence.
ACCESSION   DASPYY010000032 DASPYY010000000
VERSION     DASPYY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251891"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VEP73_00435"
     CDS             <1..576
                     /locus_tag="VEP73_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYT24938.1"
                     /translation="TVQSESEGCGGLLFGSGFPVSGNFVVTNAHVVAGTRNHRVQTNG
                     GRKVVATVVWFDPRRDLALLRAPGLGLRPLTLGDAQPPTPGAVIGYPGGGREQVVGAQ
                     VVSRTLARGRDIYSRSLVTRQIYILRATVRKGDSGGPVVDRAGRALGVVFAASTVDGQ
                     EGFALTNVELRAALSAAGGRTAPAATGACAA"
     gene            662..>2006
                     /locus_tag="VEP73_00440"
     CDS             662..>2006
                     /locus_tag="VEP73_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYT24939.1"
                     /translation="MRLAETVMPGAGPDGAPWVTFDTDPSRYRHWKLDVDGPVATLAL
                     DTREDGGLHPGYTLKLNSYDLGVDIELHDAVQRLRFEHPEVRAVVLTSAKEGVFCAGA
                     NIRMLAQAAHPFKVNFCKFTNETRNAIEDASAHSGQVWLCAVNGTASGGGYELALACE
                     HLLLVDDGASTVSLPEVPLLGVLPGTGGLTRIVDKRGVRRDHADFLCTVAEGVRGRRA
                     VEWRLVDELAPRSGLAAAARGRAEELAARSDRPDGAAGVALPPLDRELAGGRLAWPHV
                     TAELDRGSRVATLTVRGPREPQPADPAGIHAAGARWWPLAVARELDDAILLLRFNELE
                     LGTWVLRTEGDPAAVAAVDEVLLAHAGDWLVREVVLNLKRTLKRLDVTSRSLVALVEP
                     GSCFTGTLLELALAADRAFMLEGTFDEDRDGEDRDGEDREQDPGGAPAGLDTPAAV"
BASE COUNT          252 a          760 c          752 g          242 t
ORIGIN      
        1 accgtgcaga gcgagagcga gggctgcggc ggcctgctct tcggcagcgg cttcccggtc
       61 tccggcaact tcgtggtgac caacgcgcac gtggtcgccg gcacgcgcaa ccaccgggtg
      121 cagaccaacg gcggacggaa ggtcgtggct accgtggtct ggttcgaccc gaggcgcgac
      181 ctggcgctgc tgcgcgcgcc cggcctcggg ctgcggccgc tcaccctggg cgacgcgcag
      241 ccgccgacgc ccggtgccgt gatcggctac ccgggcggcg ggagagagca ggtcgtcggg
      301 gcccaggtgg tgtcgaggac gctcgcgaga gggcgcgaca tctactcccg ctccctggtg
      361 acccggcaga tctacatcct gcgggcgacg gtccgcaagg gcgacagcgg cgggccggtc
      421 gtggaccggg ccggccgcgc gctcggcgtc gtgttcgccg cctccaccgt cgacggccag
      481 gaagggttcg cgctcaccaa cgtcgagctc cgcgcggccc tgtccgcggc cggggggcgc
      541 accgcgccgg cggcgaccgg tgcgtgcgcc gcctgaggcc cgccatcgcg agacggcctg
      601 ttcacggcgc cgtctccatg gggcaggata aggggacgcg acgagctgga agaaggctgc
      661 catgcgcctt gccgagacgg tcatgccggg cgccggtccc gacggcgccc cgtgggtcac
      721 cttcgacacc gatcccagcc gctaccgcca ctggaagctc gacgtggacg ggcccgtggc
      781 caccctcgcc ctcgacacgc gggaggacgg cggcctgcac cccggctaca cgctcaagct
      841 gaactcctac gacctcggcg tcgacatcga gctgcacgac gccgtccagc ggctgcggtt
      901 cgagcacccc gaggtccgcg cggtcgtgct gaccagcgcg aaggaagggg tcttctgcgc
      961 cggcgcgaac atccggatgc tcgcccaggc cgcccacccg ttcaaggtca acttctgcaa
     1021 gttcaccaac gagacccgca acgcgatcga ggacgcgagc gcccactccg ggcaggtgtg
     1081 gctgtgcgcg gtcaacggca ccgcgtccgg cggcggctac gagctcgccc tcgcctgcga
     1141 gcacctcctg ctcgtcgacg acggcgcgag caccgtgtcc ctgcccgagg tgccgctcct
     1201 cggcgtgctg cccggcaccg gcggcctgac ccggatcgtc gacaagcgcg gggtgcggcg
     1261 cgaccacgcc gacttcctgt gcaccgtcgc ggagggggtc cgcggccggc gggcggtcga
     1321 gtggcggctg gtcgacgagc tggcgccgag gagcggcctg gccgccgcgg cccgcgggcg
     1381 cgccgaggag ctggccgccc ggtcggaccg gccggacggc gcggcgggcg tggcgctgcc
     1441 gccgctcgac cgggagctgg ccggtggccg cctcgcctgg ccccacgtca ccgccgagct
     1501 ggaccgcggc tcgcgcgtcg cgacgctcac cgtccgcggg ccgcgggagc cccagccggc
     1561 cgacccggcc ggcatccacg cggccggcgc ccgctggtgg ccccttgcgg tcgcccggga
     1621 gctcgacgac gcgatcctgc tcctgcgctt caacgagctc gagctcggca cctgggtgct
     1681 gcgcaccgag ggcgaccccg ccgccgtcgc cgccgtggac gaggtcctgc tcgcgcacgc
     1741 cggggactgg ctcgtgcgcg aggtcgtcct gaacctcaag cggacgctca agcgcctcga
     1801 cgtgacctcc cgcagcctgg tcgcgctcgt cgagccgggg agctgcttca ccgggaccct
     1861 cctcgagctg gcgcttgccg ccgaccgcgc cttcatgctg gaaggcacct tcgacgagga
     1921 ccgggacggc gaggaccggg acggcgagga ccgggagcag gacccggggg gcgcgccggc
     1981 cggcctggac acgccggcgg cggtca
//
LOCUS       DASPYY010000033         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2252016, whole genome shotgun sequence.
ACCESSION   DASPYY010000033 DASPYY010000000
VERSION     DASPYY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252016"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEP73_00445"
     CDS             complement(<1..319)
                     /locus_tag="VEP73_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HYT24940.1"
                     /translation="MSGTRWPRRLAIVGTGLLGASVGMAARAAGVPEVVGVDADRREL
                     RDALAREAITAQARSVEDAADGADLVIAATPVRALAGVLARAHKVNPTAVLSDVGSTK
                     SHLL"
     gene            complement(482..1252)
                     /locus_tag="VEP73_00450"
     CDS             complement(482..1252)
                     /locus_tag="VEP73_00450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYT24941.1"
                     /translation="MTGARPDPSPDTEPGTDGEQIRLNKVLAGAGLGSRRAVEALVRA
                     GRVTVDGEVVSDLGRRVDPARDRVALDGSQVVLDTQRRYWLLNKPAGVVSTAADEKGR
                     PTVVQLVPERPRVFPVGRLDRDTEGLLLLTNDGPLAYRLTHARYGVEKRYLAEVERLP
                     GNAISRLLAGVTLEDGLAKAERARLVAGSGRRRMVEVVMVEGRNREVRRLLDAVGAPV
                     RRLVRTAVGPIRLTGLAPGEYRPLRPEEVRRLYQAVGL"
     gene            complement(1249..>1599)
                     /gene="scpB"
                     /locus_tag="VEP73_00455"
     CDS             complement(1249..>1599)
                     /gene="scpB"
                     /locus_tag="VEP73_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYT24942.1"
                     /translation="HPGARQVVEEFLRAGQQVRITRAALETLAVVAYRQPVTRFQVAA
                     VRGVNVDGVFRTLLHRGLIREVGHDDGPGQAILYATTSQFLEQLGLDSLEELPPIKDY
                     LPEGVDLADLQEQL"
BASE COUNT          198 a          606 c          587 g          208 t
ORIGIN      
        1 caagaagatg ggatttggtg ctgccgacgt cgctcagcac cgcggtcggg ttgaccttgt
       61 gggcgcgggc gagcaccccg gcgagcgccc gcaccggcgt ggcggcgatc accaggtcgg
      121 cgccgtcggc ggcgtcctcg accgagcgcg cctgcgcggt gatcgcctcg cgggcgagcg
      181 cgtcgcgcag ctcgcgccgg tcggcgtcca ccccgaccac ctcgggcacc ccagcggcgc
      241 gggcggccat gcccaccgag gcgcccagca gcccggtgcc aacgatcgcg agccggcgcg
      301 gccacctggt cccgctcacc tgtccggctc cgtgtccggc tccaggtcac tctccatgcc
      361 actgcctcgt ttcatcgctt ggaggacaaa ggtacttgtc gacttgtcgc ttcatcgccg
      421 ccggccgctg aggctccggc tgagtccgcc agccgcgcgc gtcgccgccg cagcctcagg
      481 ctcagaggcc gacggcctga tagaggcggc ggacctcctc tggccgcagc ggccgatact
      541 cgcccggggc gaggccagtc aggcggatgg ggccgacggc ggtccgcacc agccggcgca
      601 cgggcgcgcc gaccgcgtcg agcaggcggc ggacctcgcg gttgcgcccc tcgaccatca
      661 ccacctcgac catgcgccgc cggcccgagc cggccaccag ccgggcccgc tccgccttgg
      721 ccaggccgtc ctccaaggtg acgccggcca gcagccgcga gatcgcgttg cccggcagcc
      781 gctccacctc ggcgaggtag cgcttctcca ccccgtagcg ggcatgggtg agccggtagg
      841 cgagcgggcc gtcgttggtg agcagcagca gcccctcggt gtcgcggtcc aggcgcccga
      901 ccgggaagac gcggggccgc tccggcacca gctggacgac ggtggggcgg cccttctcgt
      961 cggcggcggt gctgaccacc ccggcgggct tgttgagcag ccagtagcgg cgctgggtgt
     1021 ccagcaccac ctgggagccg tcgagggcga cgcggtcgcg ggccgggtcg acccgccggc
     1081 caaggtcgct gaccacctcg ccgtcgacgg tgacacgccc ggcgcgcacc agcgcctcga
     1141 ccgcgcgccg cgacccgagc ccggcaccgg ccagcacctt gttcagccgg atctgctcac
     1201 cgtccgtgcc cggctccgtg tccggcgacg ggtccggcct ggccccggtc acagctgctc
     1261 ctggaggtcg gcgaggtcga cgccctcggg gaggtagtcc ttgatcgggg gcagctcctc
     1321 caggctgtcg aggccgagct gttcaaggaa ctgcgatgtc gtggcgtaga ggatcgcctg
     1381 gccgggtccg tcgtcgtggc cgacctcgcg gatcaggccg cggtggagca gggtgcggaa
     1441 gaccccgtcg acgttgacgc cgcgtacggc cgcgacctgg aagcgggtca ccggctggcg
     1501 gtaggcgacc accgccaggg tctccagcgc ggcccgggtg atgcggacct gctgcccggc
     1561 gcgcaggaac tcctcgacca cctggcgggc gcccggatg
//
LOCUS       DASPYY010000034         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_4614, whole genome shotgun sequence.
ACCESSION   DASPYY010000034 DASPYY010000000
VERSION     DASPYY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1035)
                     /gene="sucD"
                     /locus_tag="VEP73_00460"
     CDS             complement(163..1035)
                     /gene="sucD"
                     /locus_tag="VEP73_00460"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYT24943.1"
                     /translation="MAILVDSQTRLVVQGLTGREGRFHALRNRDYGTNVVAGVTPGKA
                     GQDVEGVPVFNTVADAVAETGANTSLVFVPARFAVDALYEAVDGGIGTVVLVTENIPV
                     LDMTRAYNYVQAKGVALVGPNCPGVISPGKANVGIIPGEITHPGPVGLVSRSGTLTYQ
                     VMYEMAEAGVGQTTAVGIGGDPVVGSSFIDILARFEADPETEAVVMIGEIGGDEEERA
                     ARFVAERMTKPVVAYIAGFEAPPGKRMGHAGAIVSGSSGTAKAKAEALEAAGVRVGRN
                     PTEAAELAVEKVKG"
     gene            complement(1039..>1525)
                     /locus_tag="VEP73_00465"
     CDS             complement(1039..>1525)
                     /locus_tag="VEP73_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HYT24944.1"
                     /translation="KERGLNYIKLDGYVGIIGNGAGLVMSTLDVVAQAGGKAANFLDV
                     GGGASAETMAAGLEVILSDPKVRSVLVNIFGGITRGDLVAQGILDALDRLGPKVTVPL
                     VVRLDGTNAEEGRELLAESPSDKVVPAATMLEAAAKAVELAGASTAQVTTTQATDKGT
                     V"
BASE COUNT          202 a          538 c          561 g          224 t
ORIGIN      
        1 ccggaggagg cgggcgatgc cgcgtgatga cgatcggcgc atgggcaggc ctcggcgctc
       61 gaggaggtcg gtcgcgtgcg tacccgaacc atcctcgcac ggcgatcaag acccggcagc
      121 ccggcgagag gcgcgcgaat cagccaaagg cgaccagccg ggtcagcctt tgaccttctc
      181 gacggccagc tcggcggcct cggtcgggtt gcggccgacc cgcacgccgg ccgcctcgag
      241 ggcctccgcc ttggccttcg ccgtgccgct cgacccggag acgatcgcgc ccgcgtggcc
      301 catgcgcttg ccgggcggcg cctcgaagcc ggcgatgtac gcgacgaccg gcttggtcat
      361 gcgctcggcc acgaaccggg cggccctctc ctcctcgtcg ccgccgatct cgccgatcat
      421 cacgaccgcc tcggtctcgg ggtcggcctc gaaccgggca aggatgtcga tgaagctcga
      481 gccgaccacc gggtcgccgc cgatgccgac cgccgtggtc tggcccaccc cggcctccgc
      541 catctcgtac atgacctggt aggtgagcgt cccggagcgg gacacgagcc cgaccggccc
      601 tgggtgggtg atctcgccgg ggatgatgcc gacgttggcc ttcccgggcg agatgacccc
      661 cgggcagttc gggccgacca gggcgacgcc cttggcctgc acgtagttgt acgcgcgggt
      721 catgtcgagg accgggatgt tctcggtgac caggaccacc gtgccgatgc cgccgtcgac
      781 ggcctcgtag agggcgtcga ccgcgaagcg ggcgggcacg aagacgagcg aggtgttcgc
      841 gcccgtctcg gccaccgcgt cggccaccgt gttgaagacg gggacgccct cgacgtcctg
      901 gcccgccttg cccggggtca cgccggcgac gacgttggtg ccgtagtccc ggttgcggag
      961 cgcgtggaag cggccctccc gcccggtgag gccctggacg acgagccggg tctgggagtc
     1021 gacgaggatg gccatcggct acaccgttcc cttgtcggtt gcctgcgtgg tggtgacctg
     1081 cgcggtggag gcgccggcga gctcgacggc cttggcggcc gcctcgagca tggtggcggc
     1141 ggggacgacc ttgtccgagg gggactcggc gagcagctcg cgcccctcct cggcgttggt
     1201 gccgtccaga cggacgacga gcggcaccgt gaccttgggg ccgagtcggt ccagcgcgtc
     1261 gaggatgccc tgggcgacca ggtcgccgcg ggtgatgccg ccgaagatgt tcaccaggac
     1321 gctgcggacc ttggggtcgg agaggatcac ctcgaggccg gccgccatcg tctcggccga
     1381 cgccccccca ccgacgtcga ggaagttcgc cgccttgccg ccggcctggg cgaccacgtc
     1441 gagggtggac atgacgaggc cggcgccgtt gccgatgatc ccgacgtacc cgtccagctt
     1501 gatgtagttg aggccccgct ccttg
//
LOCUS       DASPYY010000035         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1549970, whole genome shotgun sequence.
ACCESSION   DASPYY010000035 DASPYY010000000
VERSION     DASPYY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549970"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1799
                     /gene="guaA"
                     /locus_tag="VEP73_00470"
     CDS             252..1799
                     /gene="guaA"
                     /locus_tag="VEP73_00470"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYT24945.1"
                     /translation="MTPTTAGHDTVLVLDFGAQYAQLIARRVRECHVYSEVVPFDVDP
                     AVVRARGTRAIILSGGPASVYEAGAPALDPGLLDLGIPVLGLCYGQQAMARALGGAVE
                     PTGVREYGRATLTAETRSVLFRDLPAEQAVWMSHGDTVTAAPPGARITARTAAAPVAG
                     FEDAARGLYGVQFHPEVAHTPRGLDLLKNFLYQAAGCRPNWTSVSIIEEQEAAVRAQV
                     GDAHVLCALSGGVDSSVAALLVHRAVGERLTCVFVDTGLLRHGEAAEVEETFRRDFKV
                     NLVHVKAAGRFLDRLAGVADPEAKRKAIGNEFIRVFEEVARDHDDVRFLVQGTLYPDV
                     VESGGQRTATIKTHHNVGGLPETMRLELVEPLRLLFKDEVRRVGVELGLPEDVVGRQP
                     FPGPGLAVRIIGEVTAERLETLRAADRVVLEEVRRAGLDAELWQWFAVLPAVRTVGVM
                     GDGRTYAYPIVIRAVTSEDAMTADFARLPWEVLERISTRVVGEVPGVNRVAYDVTSKP
                     PGTIEWE"
     gene            complement(1854..3155)
                     /gene="glyA"
                     /locus_tag="VEP73_00475"
     CDS             complement(1854..3155)
                     /gene="glyA"
                     /locus_tag="VEP73_00475"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYT24946.1"
                     /translation="MTTDQATRRDAPTDGPLAQVDPELAGLLRAEERRQVAKLPLIPS
                     ENYASRAVLEASGSVLTNKYSEGYPGRRYYEGQQLIDQVEQLAIERAKALFGVEHANV
                     QPYSGSPANLAIYLAFCQPGDTVMGMALPMGGHLTHGWNVSVTGRWFRAVQYSVRRDT
                     GRVDLDEVRDLARRERPRLLFCGGTAIPRTIDFAGFAEIAREVDAILVADIAHIAGLV
                     AGGAHPSPVGHAEVISTTTHKTLRGPRGAMLACTADRAQALDRAVFPGLQGGPHNHTT
                     AAIAVALHEAAQPAFRDYAAQVVANAARLAEVLSDRGFELVSGGTDNHLLLIDLTSKG
                     VEGKPAAQGLDRAGLVANYNTVPYDPRKPFNPSGIRLGTPSVTSRGMGLAEMDLIGRW
                     IDEGVAAVWAGDEAVLDRIAAEVRELAARFRPPGVLVHGEE"
BASE COUNT          405 a         1217 c         1150 g          452 t
ORIGIN      
        1 ctcgacgacg gcgtcgctgg cgggctccgg gctgggatcg tcggctcggt gcaaggttca
       61 ctcctcccgc gtccacgacc cggccccacg ggccagatcc ggggccgccc gcaaaccggt
      121 gcaagcaaaa agcaccacgc aggctctgcg tggtgctggg tccaggcacc agggcgggca
      181 gggcgccccg actcgttgtg ctggtcaagg tactctcgtc gggtgcggcc cgccagcggt
      241 atcctccacc gatgaccccc accaccgccg gccacgacac ggtcctcgtc ctggacttcg
      301 gcgcccagta cgcccagctg atcgcccgcc gggtgcgcga gtgccacgtc tactcggagg
      361 tcgtcccgtt cgacgtcgac cccgcggtcg tccgggcccg tggcaccagg gcgatcatcc
      421 tgtccggcgg gcccgcctcg gtgtacgagg ccggcgcgcc cgcgctcgac cccggcctgc
      481 tcgacctcgg gatccccgtg ctgggcctgt gctacgggca gcaggcgatg gcacgcgcgc
      541 tcggcggtgc ggtcgagccg accggggtgc gcgagtacgg gcgcgccacg ctcaccgcgg
      601 agacccggag cgtgctgttc cgcgacctgc cggccgagca ggccgtctgg atgagccacg
      661 gcgacacggt caccgccgcg ccgcccgggg ccaggatcac cgcccgcacc gcggccgcgc
      721 cggtggccgg gttcgaggac gccgcccgcg ggctctacgg cgtgcagttc caccccgagg
      781 tcgcccacac cccacgcggc ctcgacctcc tcaagaactt cctctaccag gcggccggct
      841 gccggcccaa ctggaccagc gtgtcgatca tcgaggagca ggaggcggcc gtccgcgccc
      901 aggtcgggga cgcccacgtg ctgtgcgccc tctcgggcgg cgtcgactcc tcggtggccg
      961 ccctgctcgt ccaccgggcc gtcggtgaac ggctgacctg cgtgttcgtt gacacggggc
     1021 tgcttcggca cggcgaggcg gccgaggtcg aggagacctt ccggcgcgac ttcaaggtca
     1081 acctggtcca cgtcaaggcg gccggccgct tcctcgaccg gctcgccggc gtggccgacc
     1141 ccgaggccaa gcgcaaggcg atcggcaacg agttcatccg cgtgttcgag gaggtcgcgc
     1201 gcgaccacga cgacgtgcgg ttcctggtcc aggggacgct ctacccggac gtggtcgagt
     1261 cgggcggcca gcggacggcc accatcaaga cccaccacaa cgtgggcggc ctgcccgaga
     1321 cgatgcgcct cgagctggtc gagccgctcc gcctgctgtt caaggacgag gtccgccggg
     1381 tcggggtcga gctcggcctg cccgaggacg tcgtcgggcg ccagcccttc cccggccccg
     1441 ggctcgccgt ccgcatcatc ggcgaggtga ccgccgagcg gctggagacg ctgcgggccg
     1501 ccgaccgggt cgtcctcgag gaggtccgcc gggccggcct cgacgccgag ctgtggcagt
     1561 ggttcgccgt gctgcccgcc gtgcgcaccg tcggcgtcat gggcgacggg cgcacgtacg
     1621 cctacccgat cgtgatccgc gcggtgacca gcgaggacgc gatgacggct gacttcgcgc
     1681 gcctcccgtg ggaggtcctc gagcgcatct ccacccgggt ggtcggcgag gtccccggcg
     1741 tcaaccgggt cgcctacgac gtcacctcga agccgccggg cacgatcgag tgggagtagc
     1801 cccctggacc ggtccggacc ggtcgacagg cccgcgcgac gaccccggcc ggcctactct
     1861 tctccgtgga ccagcacgcc cggtggccgg aaccgggcgg ccagctcgcg cacctcggcc
     1921 gcgatccggt ccagcacggc ctcgtcgccg gcccacaccg ccgccacgcc ctcgtcgatc
     1981 cagcggccga tcaggtccat ctcggccagc cccatccctc gactggtcac cgagggcgtc
     2041 ccgagccgga tcccggaagg gttgaacggc ttgcgagggt cgtaggggac ggtgttgtag
     2101 ttggcgacca gccccgcgcg gtccagcccc tgcgcggccg gcttgccctc gacccccttg
     2161 ctggtcaggt cgatcagcag caggtggttg tcggtgccgc ccgacacgag ctcgaacccg
     2221 cggtcgctga gcacctcggc cagccgcgcg gcgttggcga ccacctgggc ggcgtagtcg
     2281 cggaacgcgg gctgggcggc ctcgtgcagc gcgaccgcga tcgccgcggt ggtgtggttg
     2341 tgcggcccgc cctgcagccc cgggaagacc gcccggtcga gcgcctgcgc ccggtcggcc
     2401 gtgcacgcga gcatcgcgcc gcgcggtccc cggagcgtct tgtgcgtggt ggtggagatc
     2461 acctcggcat ggccgaccgg cgagggatgc gccccgccgg cgaccagccc ggcgatgtgg
     2521 gcgatgtcgg cgaccaggat ggcgtccacc tcgcgcgcga tctcggcgaa cccggcgaag
     2581 tcgatggtgc gcgggatcgc cgtgcctccg cagaacagca gccgcggccg ttcccgcctg
     2641 gcgaggtcac ggacctcgtc caggtccacc cggccggtgt cgcggcgcac ggagtactgc
     2701 accgcccgga accacctgcc cgtgaccgac acgttccagc cgtgcgtcag gtggccgccc
     2761 atcggcagcg ccatccccat gacggtgtcg ccgggctggc agaacgccag gtagatcgcg
     2821 aggttggcgg gcgagccgga gtacggctgc acgttggcgt gctcgacgcc gaacagcgcc
     2881 ttggcccgct cgatcgcgag ctgctcgacc tggtcgatga gctgctggcc ctcgtagtag
     2941 cgccgcccgg ggtagccctc ggagtacttg ttggtcagca ccgagccgct ggcctcgagc
     3001 accgcgcggg acgcgtagtt ctccgacggg atcaggggca gcttggcgac ctgccgtcgc
     3061 tcctcggccc tgagcagccc ggcaagctcc gggtccacct gggccagcgg cccatcggtc
     3121 ggcgcgtcgc gccgcgttgc ctggtcggtc gtcatcttcc gctctccctc cgagcctccg
     3181 gcgcgagcgt gtcgcgccat tgtctggtca tccggtccac catg
//
LOCUS       DASPYY010000036         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_567441, whole genome shotgun sequence.
ACCESSION   DASPYY010000036 DASPYY010000000
VERSION     DASPYY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567441"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..979)
                     /locus_tag="VEP73_00480"
     CDS             complement(158..979)
                     /locus_tag="VEP73_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT24947.1"
                     /translation="MGAPAAGDARRAAAGDARSLVPAVSRAAAILDELAGGEGGALGP
                     SELARRLSLPKSSVVNICNALVDAGLARRRDGGFVLGGKLAELGAAYLSAFDLVREFH
                     EACAAADPPVEETLQLAVLGDGLDVIYLARRDGSHRVRLASDVGRRLPATCTATGKAL
                     LASLPPGEAAARLPQDGGLATLTPNSIARVKALLEELERVRALGFAVDREETLEGVTC
                     VGAAVPAVTPDGDRAAVSCTLLTPRAGDDRIVLLAQRVQALAGELADRVGTARQS"
     gene            1193..2005
                     /locus_tag="VEP73_00485"
     CDS             1193..2005
                     /locus_tag="VEP73_00485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT24948.1"
                     /translation="MKELDGLAALVTGAASGIGLATARHLRRGGAWVAALDLQPVPTI
                     EGILPLVADVTDQAAVDDAVATAARQLGGLDVLVNNAGIGAVGTVEDGDEAEWNQVFD
                     VNVLGIVRTSRAALPHLRRSRYPAIVNVASIAATAGLPRRACYSASKGAVLSLTLAMA
                     ADLLADGVRVTCVTPGTIDTPWVGRLLAETADPEAERAALAARQPVGRLGSAEEVAAA
                     VAFLAAPAASFVTGIALPVDGGMAGLRVPATTAPSAASSASGPEVAGDGRPA"
BASE COUNT          288 a          857 c          885 g          275 t
ORIGIN      
        1 cggccgatga cgctctggga cgagctcggc gggcggacgt ggcgcgagcg ggcggcgtcg
       61 cggccggccg gctccgggcc ggcccgccgc tcgcgccggc gtcggcgggc ggtgcgctcc
      121 cgccgaaccg cccagcgccc cccggccccg ccggcggtca gctctgccta gccgtgccga
      181 cccggtcggc gagctccccg gcgagcgcct ggacccgctg cgccagcagg acgatccggt
      241 catcgccggc acgaggcgtg agcagcgtgc agctcaccgc ggcgcgatcc ccgtcgggcg
      301 tcaccgccgg cacggccgcg cccacgcagg tgaccccctc gagggtctcc tcgcggtcga
      361 cggcgaagcc cagcgcgcgg acccgctcca gctcctcgag cagggccttg acgcgagcga
      421 tggagttggg ggtcagcgtg gcgagccctc cgtcctgggg cagccgggcg gcggcctcgc
      481 ccggtggcag gctggccagc agcgccttgc cggtcgccgt gcacgtggcc ggcagccggc
      541 gtccgacgtc ggacgcgagg cgcacccgat ggctgccgtc ccggcgggcg aggtagatca
      601 cgtccaggcc gtccccgagc acggcgagct gcagcgtctc ctcgaccggc gggtcggccg
      661 ccgcgcaggc ctcgtggaac tcgcgcacga ggtcgaaggc tgagaggtag gcggcgccga
      721 gctccgcgag cttgccgccg agcacgaacc ccccgtctcg gcggcgcgcc aggccagcgt
      781 cgacgagcgc gttgcagatg ttgacaacgg acgacttggg cagcgagagc cggcgggcga
      841 gctcgctcgg cccgagcgcg ccgccctcgc cgccggccag ctcgtcgagg atcgccgcgg
      901 cgcggctgac cgcggggacc agcgacctcg cgtctccggc cgcggcacga cgcgcgtctc
      961 cggccgctgg ggctcccacg atggaccgcc tctctgatcg cgccgttcga gtcaagtcta
     1021 gcgttgcccg tgggaccgcc aacggcgggc ggccgtcctc ccgagaccct cttgtgccgt
     1081 cgttccctgc gctgtataac attctgaccg ctgatcagta tattgaacag cagtcctcga
     1141 cgacactgct cggcacgagc gctttctcag cggaggaagt gaggggtcgc gggtgaagga
     1201 gctggacggg ctggccgccc tggtcaccgg ggccgcgtcg gggatcggcc tggccaccgc
     1261 gaggcacctg cggcgcggcg gcgcctgggt cgccgccctc gacctgcagc cggtcccgac
     1321 gatcgagggc atcctgccgc tcgtcgccga cgtcaccgac caggcggcgg tcgacgacgc
     1381 cgtcgccacg gccgccaggc agctcggcgg cctggacgtg ctggtcaaca acgccgggat
     1441 cggcgcggtc ggcaccgtgg aggacggcga cgaggcggag tggaaccagg tcttcgacgt
     1501 gaacgtcctc ggcatcgtcc gcacgagccg tgccgcgctg ccgcacctgc gccgctcgcg
     1561 gtatccggcc atcgtgaacg tcgcctcgat cgcggcgacc gccggactgc ccaggcgggc
     1621 ctgctacagc gcgagcaagg gcgccgtgct ctcgctcacc ctcgcgatgg cggcggacct
     1681 cctcgccgac ggcgtccggg tgacctgcgt caccccggga acgatcgaca ccccgtgggt
     1741 cggccggctg ctcgccgaga cggcggaccc ggaggccgag cgggcggcgc tggccgcacg
     1801 ccagccggtc ggccgcctcg ggtcggccga ggaggtcgca gcggccgtcg cgttcctcgc
     1861 cgccccggcc gcgagcttcg tcaccgggat cgcgctcccg gtcgacggcg gcatggcagg
     1921 cctgcgcgtc ccggccacga ccgcgccgag cgccgcctcc tcggcctccg gacccgaggt
     1981 cgccggcgat gggaggccgg cgtgaccggc ggcccggtgg gaacgccggc gggcggcacc
     2041 ggccgggagg aagggggcgg tcccgaggtc ctggaggcgc cgtgggccgc ggcgaggaga
     2101 agtcagcgtg agagggagag gaggacagca tgaccaaccg tcacttccgc gggtgggtcg
     2161 cgtgcatcgc cgggctgtcg ctgttggtcg ccgcgtgcag cagcggcggc aaggggcagt
     2221 cgaagcaggc caccggcggg aagaagtacg aaggcgtctc ggtcaacgtg ctgacgttca
     2281 ccgggccgca gatcgccgag ccgct
//
LOCUS       DASPYY010000037         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_848007, whole genome shotgun sequence.
ACCESSION   DASPYY010000037 DASPYY010000000
VERSION     DASPYY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848007"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1699)
                     /locus_tag="VEP73_00490"
     CDS             complement(35..1699)
                     /locus_tag="VEP73_00490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYT24949.1"
                     /translation="MSTLVYTFRRILRRGRPAAGPDAPPVVARALTVEAPPVDIAPTD
                     PLVAYFQSATGAVDLATLELGSRALDDLRAAGVRMVVPLVSQGDLIGLVNLGPRRSEQ
                     DYSADDRKLLNDLAGHAAPAVRVAQLVREQQAEIRQRERIEQELRVAQLIQQQFLPHA
                     LPELPGWHVAAYYKPARAVGGDFYDFIELPAGQVGIVVGDVTDKGVPAALVMATTHSI
                     LRAEAPRLVSPAEVLERANDLLVTEMPAHMFVTCLYAVLDPATGRLRFANAGHNLPYL
                     RGAEGVAELRATGMPLGLMPGMHYDEKETTLRAGDGLLLHSDGLAEAHGAEREMFGFP
                     RLAKLVGDSDGGEALIDLLLGELAGFTGPEWEQEDDITLVTLQCTGAGTAGTAASALA
                     GAGAGGAGGEDDRDGRVLAEFSVPSEPGNERVAIDRVAEAVQGLGLPGARLERLKTAV
                     GEATMNAIEHGNRNRPELSVGVRVSASEADLSVRITDHGDDRSLPEPGVPDLEAKLSG
                     LQTPRGWGLFLIRNMVDDLRTSTDEAGHVVELVVHLEKGADGREHV"
     gene            1940..>2482
                     /locus_tag="VEP73_00495"
     CDS             1940..>2482
                     /locus_tag="VEP73_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24950.1"
                     /translation="MPELRLALLGPPRAERDGDPIEVDTRKAIALLAYLALTGGLQGR
                     DSLAALLWPDYDRDHARAALRRTLSVLNKALSARGHAGPVRGWLRVDRTAARLDPDGV
                     WFDVRRFRDLVAGCLEHGHPAAEVCAACVAPLAEAVALHRDDFLAGFSLRDSPSFDDW
                     QFYQAESLRRELAAALDRLTR"
BASE COUNT          298 a          925 c          912 g          347 t
ORIGIN      
        1 gatgctcgcg tcgccgcggc gacgcaccgt cgtctcaaac gtgctcacgg ccatccgctc
       61 ccttctcgag gtggacgacc agctcgacga cgtggcctgc ctcgtcggtc gacgtccgca
      121 ggtcgtccac catgttcctg atgaggaaga gcccccagcc ccgcggtgtc tgcaggccgg
      181 agagcttggc ctcgaggtcg gggacgccag gctcggggag ggaccggtcg tccccatggt
      241 cggtgatccg caccgacagg tccgcctccg aggcgctcac ccgcacgccg accgacagct
      301 cggggcggtt ccggttgccg tgctcgatgg cgttcatcgt cgcctcgccg accgcggtct
      361 tgaggcgctc gaggcgcgcc ccgggcagcc cgagcccctg gaccgcctcg gccacccggt
      421 cgatggcgac ccgctcgttc ccgggctcgc tggggacgct gaactcggcg agcacccggc
      481 cgtcgcggtc gtcttcgccc cccgccccgc cggcgccggc gccggccagc gccgacgccg
      541 cggtgccggc cgtccccgca ccggtgcact gcagggtcac gagggtgatg tcgtcctcct
      601 gctcccactc cggcccggtg aacccggcca gctcgccgag gagcaggtcg atcagcgcct
      661 cgccgccgtc ggagtcgccg accagcttgg cgagccgggg gaagccgaac atctcccgct
      721 cggccccgtg cgcctcggcc aggccgtcgg agtgcagcag caggccgtcg ccggcgcgga
      781 gggtggtctc cttctcgtcg tagtgcatcc ctggcatgag gccgagcggc atcccggtcg
      841 cgcgcagctc ggcgacgccc tccgcgcccc tgaggtaggg gaggttgtgc ccggcgttcg
      901 cgaagcggag ccggcccgtg gccgggtcga gcaccgcgta caggcaggtg acgaacatgt
      961 gggccggcat ctccgtgacc agcaggtcgt tggcccgctc cagcacctcg gcgggcgaga
     1021 cgagcctggg cgcctccgcg cgcaggatcg agtgcgtcgt ggccatcacg agggcggccg
     1081 gcacgccctt gtcggtgacg tcgccgacga cgatgccgac ctggccggcg ggcagctcga
     1141 tgaagtcgta gaagtcgccg ccgacggcgc gggccggctt gtagtaggcg gccacgtgcc
     1201 agccgggcag ctcgggcagc gcgtgcggca ggaactgctg ctggatgagc tgggcgaccc
     1261 gcagctcctg ctcgatgcgc tcgcgctggc ggatctcggc ctgctgctcg cggacgagct
     1321 gggcgacccg gacggccggg gcggcatgcc cggccaggtc gttcaggagc ttgcggtcgt
     1381 cggccgagta gtcctgctcg ctgcgccggg gcccgaggtt cacgaggccg atgaggtcgc
     1441 cctggctgac gagcgggacg accatgcgca cgccggccgc ccggaggtcg tcgagcgccc
     1501 tcgaccccag ctccagggtg gcgaggtcga ccgcgccggt cgcgctctgg aagtaggcga
     1561 ccagcgggtc ggtcggggcg atgtcgacgg gcggggcctc gaccgtgagg gcgcgcgcga
     1621 ccaccggcgg ggcgtcgggg ccggccgccg gccggccccg acggaggatc cgcctgaagg
     1681 tgtagacgag tgtgctcatc ggcgctcctt cacgcccttc tcctgtcgcg tcgttcatca
     1741 tggctccgtc gtaccaggcg accgttccgg gatcgttacg ggcggcgtta cggtgaccgg
     1801 accggatcgc cgatcctcca gctcgctgcc aaggatgacc ggttgcccgc ccgggggcca
     1861 tcccccgcct gccgttgcat cctgccgttg catcgggagg gcgcttcgcg gtatgcaacc
     1921 cgagcgaagg gagggacgcg tgccggaact gcggctcgcc ctgctcgggc ctccacgggc
     1981 cgagcgcgac ggcgacccca tcgaggtgga cacccgcaag gcgatcgccc tgctcgccta
     2041 cctcgcgctc accggcggcc tgcagggccg cgacagcctc gccgccctgc tctggccgga
     2101 ctacgaccgc gaccacgcgc gggccgccct gcggaggacc ctgtccgtgc tgaacaaggc
     2161 gctcagcgca cgagggcatg caggtccggt ccgcggctgg ctgcgggtcg accgcaccgc
     2221 ggcgcgcctg gacccggacg gcgtctggtt cgacgtgcgg cgcttccgcg acctcgtcgc
     2281 cgggtgcctc gagcacggcc acccggccgc cgaggtgtgc gccgcctgtg tcgccccgct
     2341 ggccgaggcg gtcgcgctgc accgggacga cttcctcgcc ggcttctcgc tgcgcgacag
     2401 cccgagcttc gacgactggc agttctacca ggccgagagc ctgcgccggg agctggccgc
     2461 ggcgctcgac cggctcacac gc
//
LOCUS       DASPYY010000038         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1269211, whole genome shotgun sequence.
ACCESSION   DASPYY010000038 DASPYY010000000
VERSION     DASPYY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269211"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VEP73_00500"
     CDS             complement(<1..1007)
                     /locus_tag="VEP73_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HYT24951.1"
                     /translation="MADIEIGIGKSGRRAYGFDDIAIVPSRRTRDPDDVDISWDIDAY
                     HFELPLLASAMDGVVSPRIAIELGRLGGLGVLNLEGLWTRYADPDAVYQEIAGLPDAE
                     ATKRMQAIYREPIDPDLLGQRVKEIKDAGGIAAASLTPQRVERYHQYALDAGLDILVI
                     QGTVVSAEHVSTTSEPLNLKTFIARYDLPVIVGGCASYSTALHLMRTGAAGILVGVGP
                     GAACTTRGVLGIGVPQATALADAAGARVRHLEETGRYVQVIADGGMRTGGDVAKAIAC
                     GADAVMLGSPLARAVEAPGRGYHWGMATFHPTLPRGARVKVDQVASMEEIVVGPAREN
                     DG"
     gene            1194..1676
                     /locus_tag="VEP73_00505"
     CDS             1194..1676
                     /locus_tag="VEP73_00505"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HYT24952.1"
                     /translation="MPDPLDPFEPDDAQPEPIDEEERASLLEDLADLAEFREALEPRG
                     FRGVVIPCPDCEEDHFFSWDLLRENLEHILEQGEPRVHEPAFEPSVDHYVTWDYGKGF
                     VDGLLEGEHENGPLRDGLTSPTEAARRIRRALTTRGLTEDEVTEVLSEGGLPAGSDLE
                     "
     gene            complement(1688..2491)
                     /locus_tag="VEP73_00510"
     CDS             complement(1688..2491)
                     /locus_tag="VEP73_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HYT24953.1"
                     /translation="MSTTWAPTRRAAFFDVDKTLLPGSSLYLLARGMYRRGFYTWRDI
                     VGFALGQITFRVTGAEGRRGLEAARESALAFIVGKSQAEMVQLGHDIVVEVIGPRIYP
                     GMRRVIDVHHANGDRTYLVTAAPRELAERIAAYLGMDGALGTEGELVDGIYTGRLTGP
                     VLHGPAKLDAVVKLAAEEGIDLRDSSAYSDSVNDRPMLEGVGHPVAVNPDRQLRDLAA
                     QRGWPVQDFRKRRRLWLVWVPSGLAGVGAALAAVMLRRRGGHDRFLGAP"
     gene            complement(2488..3432)
                     /locus_tag="VEP73_00515"
     CDS             complement(2488..3432)
                     /locus_tag="VEP73_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HYT24954.1"
                     /translation="MPRAVITGGAGFLGSHLCRRLLAEGFDVVCMDSLLTGRAANVED
                     LVGKHFRLVNVDVTDYVHVSGPVDAVLHFASPASPIDYLRYPIQTLKVGALGTHRALG
                     LAKDKGARFLLASTSEVYGDPQVHPQPETYWGHVNPIGPRGVYDEAKRFAEALAMAYQ
                     RTHGMQIRIARIFNCYGWRMRIDDGRAFCTFAVQALRGEPLTVHGDGSQTRSLCYVDD
                     LVEGFWRLLWSDLEGPVNLGNPQEVTILELATMVAEAAGAEVRVDYRPRPVDDPEVRR
                     PDITRAMELLDWKPTVTLEEGIARTLPWFRRALEAEQR"
BASE COUNT          497 a         1288 c         1275 g          486 t
ORIGIN      
        1 ccgtcgttct cgcgcgcggg cccaaccacg atctcctcca tgctcgccac ctggtcgacc
       61 ttcacgcggg caccgcgcgg gagcgtcggg tggaatgtcg ccatccccca gtggtacccg
      121 cggccgggcg cctcgacggc cctggccagc ggggacccga gcatcacggc gtccgcgccg
      181 caggcgatcg ccttggcgac gtcgccgccg gtgcgcatgc ccccgtcggc gatgacctgg
      241 acgtagcggc ccgtctcttc gaggtggcgg acgcgggcgc cggcggcgtc ggccagcgcg
      301 gtcgcctggg ggaccccgat gccgaggacg cccctggtcg tgcaggcggc gcccgggccc
      361 acgccgacga ggatgccggc cgccccggtc cgcatcaggt gcagcgcggt cgagtaggag
      421 gcgcagccgc cgacgatcac gggcaggtcg tagcgggcga tgaaggtctt gaggttgagc
      481 ggctcgctcg tggtcgagac gtgctcggcc gagaccacgg tgccctggat gacgaggatg
      541 tccagcccgg cgtcgagcgc gtactggtgg taccgctcga cccgctgggg ggtgagcgag
      601 gccgcggcga tcccgccggc gtccttgatc tccttgaccc gctggccgag caggtcgggg
      661 tcgatcggct cgcggtagat cgcctgcatg cgcttggtcg cctcggcgtc gggcaggccg
      721 gcgatctcct ggtagaccgc gtccgggtcg gcgtagcggg tccacaggcc ctcgaggttg
      781 agcacgccga gcccgcccag gcggccgagc tcgatcgcga tccggggcga gacgaccccg
      841 tccatggccg aggccagcag cggcagctcg aagtgatagg cgtcgatgtc ccaggagatg
      901 tccacgtcgt ccgggtcgcg cgtccgcctg ctcggcacga tggcgatgtc gtcgaagccg
      961 taggcgcgcc ggccgctctt gccgattccg atctctatgt cagccaacgg tctcccccat
     1021 cgggcggaat gcaaaatgga cccgaccggg gcgcggtggg tccaggtagt ggtgcatacc
     1081 tgaacctacc aacggtcctc cccagggtca aacgcggcct ccgcagccca ggcaacacgc
     1141 tgtaccatgc gagggttcac aagaagagcg atcgaccccg cacagaggtg cccgtgcctg
     1201 accccctcga cccgttcgag cctgacgacg cgcaacccga gcccatcgac gaggaggagc
     1261 gtgcctccct gctcgaggac ctggccgacc tggccgagtt ccgtgaggcg ctcgagccgc
     1321 gcggcttccg gggcgtggtc atcccctgcc cggactgcga ggaggaccac ttcttcagct
     1381 gggacctcct ccgcgagaac ctcgagcaca tcctcgagca gggcgagccc cgcgtccacg
     1441 agccggcctt cgagccgtcc gtcgaccact acgtgacctg ggactacggc aagggcttcg
     1501 tcgacggcct gctcgagggc gagcacgaga acggcccgct ccgcgacggc ttgacctcgc
     1561 ccaccgaggc cgcccggcgc atccgccgcg ccctcaccac ccgcggcctg accgaggacg
     1621 aggtcaccga ggtgctcagc gagggcggcc tgccggccgg cagcgacctc gagtagcccc
     1681 gccgcggtca gggcgcgccg agaaaccggt cgtgtcctcc gcggcggcgg agcatgacgg
     1741 ccgccagggc ggcgccgacg ccggccagcc ccgaggggac ccacaccagc cagaggcggc
     1801 ggcgcttgcg gaagtcctgg accggccagc cgcgctgggc ggccaggtcc cgcagctggc
     1861 ggtcggggtt gaccgccacc gggtggccga cgccctcgag catcggacgg tcgttcaccg
     1921 agtccgagta cgcgctcgag tcgcgcaggt cgatgccctc ctcggcggcg agcttgacga
     1981 ccgcgtcgag cttggccggg ccgtggagca ccggcccggt gagccgcccg gtgtagatcc
     2041 cgtcgacgag ctcgccctcg gtcccgaggg cgccgtccat gccgaggtag gcggcgatcc
     2101 gctcggccag ctcccggggc gcggcggtga ccaggtaggt gcggtcgccg ttcgcgtggt
     2161 ggacgtcgat gacccgccgc atgcccgggt agatgcgggg gccgatcacc tccaccacga
     2221 tgtcgtggcc gagctggacc atctcggcct gggacttgcc cacgatgaac gcgagcgccg
     2281 actcgcgggc cgcctcgagc ccccgcctgc cctccgctcc ggtgaccctg aacgtgatct
     2341 gcccgagcgc gaacccgacg atgtcccgcc aggtgtagaa cccgcgccgg tacatgcccc
     2401 gggcgagcag gtacaggctc gaccccggca gcagggtctt gtcgacgtcg aagaacgccg
     2461 cccggcgggt cggtgcccac gtcgtgctca ccgctgctcg gcttccagcg ccctgcggaa
     2521 ccacggcagc gtccgcgcga tgccctcctc gagcgtgacc gtcggcttcc agtcgagcag
     2581 ctccatggcg cgggtgatgt cgggccgccg cacctcgggg tcgtcgaccg gccgcggccg
     2641 gtagtcgacc cgcacctcgg ccccggccgc ctcggcgacc atcgtggcga gctcgaggat
     2701 ggtgacctcc tgcgggttcc cgaggttgac gggcccctcg aggtcgctcc agagcagccg
     2761 ccagaaccct tccaccaggt cgtcgacgta gcacagcgac cgggtctggc tgccgtcccc
     2821 gtgcacggtg agcggctccc cgcgcaacgc ctgcaccgcg aacgtgcaga acgcccgccc
     2881 gtcgtcgatt ctcatgcgcc acccgtagca gttgaagatt cgcgcgatcc gaatctgcat
     2941 gccatgggtg cgctggtagg ccatggcgag ggcctccgcg aagcgcttgg cctcgtcgta
     3001 gacgccgcgg gggccgatcg ggttgacgtg gccccagtac gtctcgggct gcgggtggac
     3061 ctgggggtcg ccgtacacct cgctcgtcga ggcgagcagg aagcgggcgc ccttgtcctt
     3121 ggccagcccg agcgcccggt gcgtgccgag cgctcccacc ttcagggtct ggatcgggta
     3181 gcggaggtag tcgatggggc ttgccggcga cgcgaagtgc agcacggcgt cgaccggccc
     3241 ggagacgtgg acatagtcgg tgacgtcgac gttgaccagg cggaagtgct tgccgacgag
     3301 gtcctcgacg ttcgccgccc gcccggtcag caggctgtcc atgcagacca cgtcgaagcc
     3361 ctcggcgagc agccggcggc agagatggga gccgaggaaa ccggcgcccc cggtgatgac
     3421 ggccctcggc atggtgctca gccccgggcg tcggcgggcg gcgtcacgac cgggcgcccc
     3481 acgccggcca cggtgaaccc ctcggcgacg aacgcggcca cgtcgaggaa gttgcgcgcg
     3541 tcgacg
//
LOCUS       DASPYY010000039         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_5249, whole genome shotgun sequence.
ACCESSION   DASPYY010000039 DASPYY010000000
VERSION     DASPYY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5249"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VEP73_00520"
     CDS             complement(<1..515)
                     /locus_tag="VEP73_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24955.1"
                     /translation="MAACLGCLLTVAVGVTTMSPAVAGGSTRTISSSGSTSFSSRPSG
                     SDTLQWPELAGGDAAEEGAAPYDGTIVNRSYSHGTAKGPATSSARKAKSNPELVTSFD
                     GLTLRQQRLANGGNQFTVEPPDQGLCVGNGYVLESVNSVLNVYGTSGDSKLGVVDLNS
                     FYGYPAQFNRTT"
     gene            complement(711..>1529)
                     /locus_tag="VEP73_00525"
     CDS             complement(711..>1529)
                     /locus_tag="VEP73_00525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT24956.1"
                     /translation="GVTFFDTADVYGAGHSERVLGRALAGVRDQVVVATKFGNTFDER
                     TRQMGEPDVSPAYVRRACEASLRRLGTDRIDLYQLHVGHLPAGEAEAALEALEALARD
                     GLVRAYGWSTDDPGCARLFAGRPHYAAVQFDFNVLLDAPEMVALCDELDLAGINHGPL
                     AMGLLTGKFHAGSRLGADDVRGDAAPGWMRYFTDGRPSPEWLERLAAIRDVLTSGERT
                     LAQGAIAWIWARSGRTVPIPGFRTVRQVEENAGAMERGPLTAEQLADVDALLGR"
BASE COUNT          205 a          557 c          549 g          218 t
ORIGIN      
        1 gtggtgcggt tgaactgcgc cgggtagccg tagaaggagt tcaggtccac gaccccgagc
       61 ttcgagtcgc cgctggtgcc gtacacgttg aggactgagt tgaccgattc gagcacatag
      121 ccgttgccca cgcacagacc ctggtccggc ggctcgacgg tgaactggtt gccgccgttg
      181 gccagccgct gctggcgcag cgtgagcccg tcgaagctcg tcacgagctc gggattggac
      241 ttcgccttcc gcgcgctcga cgtagcgggg cccttggccg tcccgtgcga gtagctccgg
      301 ttgacgatcg tgccgtcgta cggggccgcg ccctcctcgg ctgcgtcgcc gccggcgagc
      361 tccggccact gcagcgtgtc cgagcccgac gggcggctcg agaagctggt gctgcccgag
      421 ctcgagatgg tccgcgtcga gccgcccgca accgcggggc tcatggtggt gacgcccacg
      481 gcgacggtca acaggcagcc gagacaggcc gccacgagag ctcttgcccg catccccaac
      541 gccccctcac ttcgggccct cgcggtcgtc ttgcgggccg acgctacctc ggcacacccg
      601 aaatgacaag acttgcgtag aaaggcgggc ttgcgaggca cgtcgggcaa gcatgcccaa
      661 ggcccagtcg tgccattcca ggccggcggg accgcttccc gtcccgccgg tcagcggcca
      721 agcagcgcgt cgacgtcggc cagctgctcg gcggtcagcg gcccgcgctc catcgccccg
      781 gcgttctcct cgacctggcg cacggtccgg aagccgggga tggggacggt ccgcccgctc
      841 ctcgcccaga tccacgcgat ggcaccttgc gcgagcgtcc gctcgccgct ggtgagcacg
      901 tcgcggatgg ccgcgagccg ctcgagccac tccgggctcg gcctgccgtc ggtgaagtac
      961 cgcatccacc cgggcgccgc gtccccgcgc acgtcgtccg ccccgaggcg gctgccggcg
     1021 tggaacttgc cggtgagcag ccccatggcc agcgggccgt ggttgatgcc ggccaggtcg
     1081 agctcgtcgc acagcgcgac catctcgggg gcgtccagca gcacgttgaa gtcgaactgg
     1141 acggcggcgt agtgcggccg cccggcgaac aggcgggcgc atcccgggtc gtcggtgctc
     1201 cacccgtacg cgcggacgag gccgtcgcgg gccagcgcct ccagggcctc gagcgcggcc
     1261 tcggcctctc cggcggggag gtgcccgacg tggagctggt acaggtcgat gcggtcggtg
     1321 cccaggcggc gcagggacgc ctcgcaggca cggcggacgt acgccggcga gacgtcgggc
     1381 tcacccatct ggcgggtgcg ctcgtcgaac gtgttgccga acttggtcgc gaccaccacc
     1441 tggtcccgca ccccggccag ggcgcggccg aggacccgct cgctgtggcc cgccccgtag
     1501 acgtcggcgg tgtcgaagaa ggtgacgcc
//
LOCUS       DASPYY010000040         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1550306, whole genome shotgun sequence.
ACCESSION   DASPYY010000040 DASPYY010000000
VERSION     DASPYY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550306"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1574)
                     /locus_tag="VEP73_00530"
     CDS             complement(<1..>1574)
                     /locus_tag="VEP73_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYT24957.1"
                     /translation="LRNMLYEGSLTLAGLRFVVMDEVHYLQDRVRGAVWEEVLINLPV
                     TVKVAALSATVSNAEEFGEWLESTRGPTEVVIEERRPVPLDNYYLVGRELHPLLVRRD
                     DGDVAPNPALARRGEREWAPSPRARDSGRRGQAGRQGAGRGWLPSRVEVVDLLEAERL
                     LPAICFIFSRAGCDQALAQCRAANIRLTTQAERLEINEFLELRVAALDPADLDALGYP
                     ELAESIGRGVASHHAGMLPLFKEAVEELFAQGLVKVVFATETLSLGINMPARTVVIEK
                     LSKWQGERHELLTPGEYTQLTGRAGRRGIDRRGAAVVLYQPYIPFERITGLASTRTYP
                     LASSFRPSYNMAVNLIANYEREEAERLLASSFAQFLADRTVHGAEQTLARNAEYLAGY
                     RAAAACDRGDADEYWELRSRIRAREGALADADRRARGDQTAEAVRALTPGTVVQLPGG
                     RRRGLAAVLGPAGGRDGSTGVLVLTEDRRVRRVAVRDFGEAPVPVGRIAMPRTLSPKS
                     PRFKARVAEALAQLDLP"
BASE COUNT          196 a          596 c          579 g          203 t
ORIGIN      
        1 ggcaggtcga gctgggcgag cgcctcggcc accctggcct tgaaccgggg gctcttcggg
       61 gagagcgtgc gcggcatcgc gatcctgccg accggcaccg gcgcctcccc gaagtcgcgt
      121 accgcgacgc ggcgcacccg ccggtcctcg gtcagcacga gcacgccggt cgagccgtcc
      181 cgcccccccg ccggcccgag cacggcggcg agcccccggc gccggccccc gggcagctgg
      241 acgaccgtgc ccggggtgag cgcgcggacc gcctcggccg tctggtcgcc gcgggccctg
      301 cggtcggcgt cggcgagcgc gccctcccgc gcccggatcc ggctgcgaag ctcccagtac
      361 tcgtcggcgt cgccccggtc gcatgctgcg gcggcgcggt agcccgcgag gtactcggcg
      421 ttgcgggcga gggtctgctc cgcgccgtgc acggtccggt cggccaggaa ctgggcgaac
      481 gaggaggcga gcaggcgctc ggcctcctcc cgctcgtagt tggcgatgag gttcaccgcc
      541 atgttgtagg agggccggaa cgaggaggcg agcgggtagg tccgggtcga cgcgaggccg
      601 gtgatccgct cgaaggggat gtagggctgg tagagcacga ccgcggcacc ccggcggtcg
      661 atcccgcgcc ggcccgcccg cccggtgagc tgggtgtact cccccggggt gagcagctcg
      721 tggcgctccc cctgccactt cgacagcttc tcgatcacca ccgtgcgggc cggcatgttg
      781 atcccgagcg acagcgtctc ggtcgcgaag accaccttga cgagcccctg ggcgaacagc
      841 tcctcgaccg cctccttgaa cagcggcagc atgccggcgt ggtggctcgc gaccccgcgc
      901 ccgatcgact cggccagctc ggggtagccg agggcgtcca ggtcggccgg gtcgagggcg
      961 gccacccgca gctcgaggaa ctcgttgatc tcgagccgct cggcctgggt cgtcaggcgg
     1021 atgttggccg cccggcactg ggccagggcc tggtcgcagc ctgctcgcga gaagatgaag
     1081 cagatggcgg gcagcagccg ctcggcctcg agcaggtcga cgacctcgac ccgggacggc
     1141 agccagccgc gcccggcccc ctggcggccg gcctggccgc gccggccgct gtcccgggcg
     1201 cggggcgacg gcgcccactc ccgctctccg cgccgggcca gggccgggtt cggggccacg
     1261 tcgccgtcgt cccgccgcac cagcagcggg tgcagctcgc ggccgacgag gtagtagttg
     1321 tccaggggca ccggccggcg ctcctcgatc accacctcgg tcggcccccg ggtcgactcg
     1381 agccactcgc cgaactcctc ggcgttggac accgtcgccg acagcgcggc caccttgacc
     1441 gtcaccggca ggttgatgag gacctcttcc cacaccgcgc cgcgcacccg gtcctgcagg
     1501 tagtgcacct cgtccatgac cacgaacctg aggccggcga gggtcaggct gccctcgtag
     1561 agcatgttgc gcag
//
LOCUS       DASPYY010000041         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1410131, whole genome shotgun sequence.
ACCESSION   DASPYY010000041 DASPYY010000000
VERSION     DASPYY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410131"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2059
                     /locus_tag="VEP73_00535"
     CDS             <1..>2059
                     /locus_tag="VEP73_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT24958.1"
                     /translation="LARLVPVVAERARRRLIDEALALSEQLDDAGTVTFWIPEGARGE
                     LLAELQRRGFNWLRVQVVIIARAVLDARQRVTAAIPPALARWAELAASVEADRTDGST
                     RQVAAQLDRRIDGLLDVGDTGQALTWLDTARALAPLLGGEFEVSVLANTRRVELVHRR
                     WLDRRRLERFLHRDEQIVAFGALLRQPAAEGDPWALHYLGVGGVGKTMLIRYLTAVAA
                     PAANAVTSRIDFDHLNPDFPRRRPAQILVELATDLEAQAQDSRLDYLFRRAWEYLQEI
                     NARARPAAVADDDFAAALRAFAGFLQALDRPVVLVLDTCEELEKFRPAGGRLEQLEAT
                     FQILEALHQQVPALRVVLAGRRPLARAGHGWRLPPGPAGQGNVLLPESKPYLLLHDVR
                     GFDRTEAERFLINHAGLRADRALLEAILERSPDPGSAADLFWDDGHRPPQVPRYNPFD
                     LDLYAGWVRAEPGMDAAALRGGGSDHYVEARIVGRLKNPEVERLLPAVLALRRFDRDM
                     LRLAFDGAEDALVDAERELASSEWIDVQPDAALDTTFLEVDRSLHPRLEAHFQRERRG
                     ELATASRRLAPGLAAMVDSRRPDRLSVEHVAAALRLLDPPDAAWLVDRLAMRVIEDAA
                     WGWAATVGERLLALDSPLADHAHPAAAGLRAMLASALLHQDPDRDLVELWAVVEQTAA
                     NHPD"
BASE COUNT          257 a          835 c          704 g          263 t
ORIGIN      
        1 ctggcccgcc tggtcccggt cgtggcggag cgcgcccgcc ggaggctgat cgacgaggcg
       61 ctcgcgctct cggagcaact cgacgacgcc gggaccgtca ccttctggat cccagaggga
      121 gcgcgcggcg agctgctcgc ggagctgcag cgccgcgggt tcaactggct gcgggtgcag
      181 gtcgtgataa tcgcgcgtgc tgtgctcgac gcgcggcagc gcgtcaccgc cgcgatcccc
      241 ccggccctag cacgctgggc ggagctggcc gcgagcgtcg aagcggaccg cactgacggc
      301 agcacccgcc aggtcgccgc gcaactcgac cggcgcatcg acgggctgct cgatgtcggg
      361 gacaccgggc aggcgctcac ctggctcgac acggccaggg cgctcgctcc gctgctcggc
      421 ggggagttcg aggtcagcgt gctggccaac acccgccggg tggagctggt gcatcgccgc
      481 tggctggacc ggcggcggct ggagcggttc ctgcaccgcg acgagcagat cgtggcgttc
      541 ggggcgctgc tgcgccagcc ggcggcggag ggcgacccct gggcgctgca ctacctcggc
      601 gtcggcgggg tcggcaagac catgctgatc cgctacctca ccgccgtggc ggcgcccgcg
      661 gcgaacgcgg tcaccagccg catcgacttc gaccacctga accctgactt cccccggcgc
      721 cgccccgccc agatcctcgt cgagctggcc accgacctgg aggcgcaggc gcaggactcc
      781 cggctcgact acctgttccg ccgcgcctgg gaatacctcc aggagatcaa cgcccgcgcc
      841 cggccggcgg cggtcgccga cgacgacttc gccgcggcgc tgcgggcgtt cgccgggttc
      901 ctgcaggcgc tcgaccgccc ggtggtgctg gtcctggaca cctgcgagga gctggagaag
      961 ttccgcccgg cgggtgggcg gctggagcag ctcgaagcga ccttccagat cctggaggcc
     1021 ctccaccagc aggtccccgc cctgcgggtg gtgctcgccg gccgcaggcc cctcgcccgc
     1081 gccgggcacg gctggcgcct gccgccggga cccgccggcc agggcaacgt gctgctcccc
     1141 gagtccaagc cctacctgct gctccacgac gtccgcggct tcgaccgtac cgaggccgag
     1201 cgcttcctca tcaaccacgc cggcctgcgc gccgaccggg cgttgcttga ggcgatcctg
     1261 gagcgcagcc ccgacccggg cagcgccgcc gacctgttct gggacgacgg ccaccgcccg
     1321 ccgcaggtgc cccgctacaa ccccttcgac ctggacctgt acgccgggtg ggtccgcgcc
     1381 gagcccggca tggacgctgc ggcgctacga ggcggtggca gcgaccacta cgtcgaggcc
     1441 cgcatcgtcg gccgcctgaa gaaccccgag gtcgagcgcc tcctcccagc ggtgcttgcg
     1501 ctgcgccgct tcgaccgcga catgctccgc ctcgccttcg acggcgccga ggacgcgctc
     1561 gtcgacgccg aacgcgaact ggcctcctcc gagtggatcg acgtccagcc cgacgccgcc
     1621 ctggacacca ccttcctcga agtcgaccgc agcctccatc cccgcctgga ggcccacttc
     1681 cagcgcgagc gccgtggtga gcttgccacc gccagccgcc gcctcgcccc gggcctcgcc
     1741 gccatggtcg acagccgccg ccccgaccgc ctcagcgtcg agcacgtggc ggcggcgctg
     1801 cgcctgctgg acccccccga cgccgcgtgg ctggtcgacc ggctcgccat gcgcgtcatc
     1861 gaagacgccg cctggggctg ggccgccacc gtcggcgaac gcctcctcgc gctggactcc
     1921 ccgctggccg accacgccca ccccgccgcc gccggcctcc gggccatgct tgcctcggcc
     1981 ctcctccacc aggaccccga ccgcgacctc gtcgagctgt gggccgtggt cgagcagacc
     2041 gccgccaacc accccgacc
//
LOCUS       DASPYY010000042         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1691281, whole genome shotgun sequence.
ACCESSION   DASPYY010000042 DASPYY010000000
VERSION     DASPYY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691281"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VEP73_00540"
     CDS             <1..391
                     /locus_tag="VEP73_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYT24959.1"
                     /translation="MDIVRAPLRWLGRVTLSAMFIAGGVDAFRQPEPRAPKAASLGIP
                     SPELAVRANGAAMVAAGVALALGYRRRTAAAVLAGLLVPTTLAGHPFWAEQDPVARMQ
                     QRVHFLKNLGLLGGALLVLGERPKPSR"
     gene            439..1530
                     /locus_tag="VEP73_00545"
     CDS             439..1530
                     /locus_tag="VEP73_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT24960.1"
                     /translation="MVVRPVRAGEAAALWAVRLRALADAPEAFGQTLDEARASGPASF
                     EELVADAVAGRQFVAVAEARRAERAGELVGMTGVRRSAQEGKFAHRAVVWGVWVAPEA
                     RGLGVAGAMLDQAIDWCRASGVGTVQLTVVVGNTAALRLYRRAGFVVYGTDPDAMRDG
                     GRSVPEHLMLLRLDAEPVAVRAVRAGEGPALWALRLRSLAESPDTFGQTVAEAEATGG
                     TPIVRLVAEAVAGHAFAGVAEQGGRLVGMAVVRPGERAANRHRGVLFGVFVAPEARGQ
                     GVATRLVEAAVAWCRGRGLRSVELWVRADNTAARAVYERAGFVRRGLDPDRFRTAGAP
                     VAEHLMILSLSHETIPSPPQDRGDERSKQ"
     gene            1527..2060
                     /locus_tag="VEP73_00550"
     CDS             1527..2060
                     /locus_tag="VEP73_00550"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT24961.1"
                     /translation="MTEQERPGPAGTPPASRPDLPASYGVPPTAEGMLPWSHAVERIR
                     AARNYWVSTTRPDGRPHAVPVWGVWVDETFFHGGGPDTRKARNLAVNPAVSVHLESGD
                     EVVIVEGTVDRITADHPDQDLLRRIDDAYEAKYGMRHGTPVWALRPRVAFAWRLEGFP
                     TTMTRWTFAPRATGEVP"
     gene            complement(2191..>2539)
                     /locus_tag="VEP73_00555"
     CDS             complement(2191..>2539)
                     /locus_tag="VEP73_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT24962.1"
                     /translation="DVLVNNAGLAAGLAPIAEGDERDWAVMLETNVLGLLRVTRAFLP
                     LLRAAPHAHIVNLGSIAGGEVYPGIQPLTADDVADCVAFVVTRPPHVDIDRLVVRPVA
                     QAAAYMVARADTR"
BASE COUNT          294 a          941 c          998 g          306 t
ORIGIN      
        1 gatggacatc gtccgcgcac cgctccgctg gctgggtcgc gtcaccttgt ccgcgatgtt
       61 catcgcgggc ggcgtcgacg ccttccggca gcccgagcct cgcgcgccca aggcggccag
      121 cctcggcatc ccgagcccgg agctcgcggt cagggcgaac ggcgcggcga tggtcgcggc
      181 cggggtcgcg ctggccctcg gctaccgccg gcggacggcc gcggccgtgc tggccggcct
      241 cctggtcccg accacgctgg ccggccaccc gttctgggcg gagcaggacc ccgtcgcccg
      301 catgcagcag cgcgtccact tcctcaagaa tctcggcctg ctcggcggcg ccctgctcgt
      361 cctcggcgag cgcccgaaac cgtcccgctg acccgttcgc gggcccgtca ggcaggcgtg
      421 ccgaacggaa caggaagcgt ggtcgtccgc ccggtccggg cgggcgaggc ggccgccctc
      481 tgggcggtcc gcctgcgcgc gctggccgac gcgcccgagg cgttcggcca gacgctcgac
      541 gaggcgaggg ccagcggccc ggcgtcgttc gaggagctgg tcgctgacgc ggtcgccggc
      601 cgccagttcg tcgccgttgc ggaggcacgc cgggcggagc gggccggcga gctggtcggc
      661 atgacaggcg tgcgccgcag cgcccaggaa ggcaagttcg cccaccgggc cgtcgtctgg
      721 ggcgtctggg tggccccgga ggccaggggc ctcggcgtgg ccggggcaat gctcgaccag
      781 gcgatcgact ggtgccgcgc cagcggggtc ggtacggtgc agctcaccgt ggtggtcggc
      841 aacacggccg ccctccggct ctaccggcgg gcaggcttcg tcgtctacgg caccgacccc
      901 gacgccatgc gcgacggcgg ccggtcggtc cccgagcatc tcatgctgct gcgcctggac
      961 gccgagccgg tcgccgtccg ggcggtccgg gcgggggagg ggccggcgct ctgggccctg
     1021 cggctgcgct cgctcgccga gtcgcccgac accttcggcc agaccgtcgc ggaggccgag
     1081 gccaccggcg gcacgccgat cgtgcggctg gtggccgagg cggtcgccgg gcacgccttc
     1141 gcgggcgtcg ccgagcaggg cggccggctg gtcgggatgg cggtcgtgcg gcccggcgag
     1201 cgagccgcca accgccaccg cggcgtgctg ttcggcgtct tcgtcgcccc cgaggcccgc
     1261 ggtcagggcg tcgcgacccg gctcgtggag gccgcggtcg cctggtgccg cgggcgcggg
     1321 ctgcgctcgg tcgagctgtg ggtgcgggcc gacaacacgg ccgcgcgggc cgtctacgag
     1381 cgggccggct tcgtccgccg cggccttgac cccgaccgct tccggacggc cggcgccccg
     1441 gtcgccgagc acctcatgat cctgtcactg tcccacgaga cgatcccgtc gccgccgcag
     1501 gaccgcgggg acgagaggag caagcaatga ccgagcagga gcggccgggg ccggcgggga
     1561 cgccccctgc gagccgacca gacctcccgg cgagctacgg cgtcccgccg acggccgagg
     1621 ggatgctgcc ctggagccac gcggtcgagc ggatcagggc cgcccgcaac tactgggtgt
     1681 cgacgacccg gcccgacggg cgaccgcacg cggtgccggt gtggggggtg tgggtcgacg
     1741 agacgttctt ccacggcggc gggccggaca cccgcaaggc ccgcaacctc gcggtcaacc
     1801 cggcggtgag cgtgcacctg gagagcggcg acgaggtcgt gatcgtcgag gggacggtgg
     1861 accgcatcac cgccgaccat cccgaccagg acctgctgcg ccgcatcgac gacgcctacg
     1921 aggcgaagta cggcatgcgg cacggcaccc cggtgtgggc gctgcgccca cgggtcgcgt
     1981 tcgcgtggag gctcgagggg ttccccacga cgatgacccg ctggaccttc gccccacggg
     2041 cgaccggcga ggtgccgtag ccgcggggct cccggggctc ggtcccccac tcgccctccg
     2101 tacgttatgt caaccgatgg gggagcgggc ggcgggcggc gctcccgcgc ggagtcccgg
     2161 cgccggcggc tcgccggacc gggcggcggc tcagcgggtg tccgcgcggg cgaccatgta
     2221 cgcggcggcc tgggcgaccg ggcggacgac gagccggtcg atgtcgacgt gtggtgggcg
     2281 cgtcaccacg aacgcgacgc agtcggcgac gtcgtcggcg gtgagcggct ggatgcccgg
     2341 gtagacctcc ccacccgcga tcgacccgag gttgacgatg tgcgcgtggg gcgcggcccg
     2401 cagcaggggg aggaaggcgc gggtgacccg cagcagcccg agcacgttgg tctcgagcat
     2461 caccgcccag tcccgctcgt cgccctcggc gatcggcgcg agccccgccg cgagcccggc
     2521 gttgttgacc agcacgtcc
//
LOCUS       DASPYY010000043         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2533741, whole genome shotgun sequence.
ACCESSION   DASPYY010000043 DASPYY010000000
VERSION     DASPYY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533741"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VEP73_00560"
     CDS             complement(<1..1239)
                     /locus_tag="VEP73_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT24963.1"
                     /translation="MTRTVASIIGGQPRHGTAGARRRALPNPARTGQVTSEAELAGAG
                     VFLEACRAAQAARRAWAATPAPVRGRAIQQAGRLVESNAEALARIVTDEIGKPYAESL
                     GEVQEVVDTCSFFLGEGRRLYGQTVPSEMPDKQLFTFRVPVGVAAVVTAGNFPVAVPS
                     WYLVPALVCGNPVVWKPAEYAPASAEALTRLFLHAGVPADVLHLVVADGPTAFEGLEL
                     ALAAGLVDKVGFTGSSDVGRRIGALTGRHLQSPCLELGGKNPLVVMPDADLDLAVEGA
                     LFSGFGTAGQRCTSLGTVVVHESVHDDFLARFTAATEAAAIGDPYADVLYGPMLNHRF
                     LERFLGWLDLVEGHHRVSGSTGVGRITRANPRAGFVGDPDAGLYCHPTIVDGVTATDK
                     LARTETFGPIVGVASFADDDG"
BASE COUNT          192 a          589 c          568 g          192 t
ORIGIN      
        1 cccgtcgtcg tcggcgaagg acgccacgcc cacgatgggg ccgaacgtct ccgtgcgggc
       61 cagcttgtcg gtggcggtga cgccgtcgac gatggtcgga tggcagtaca ggcccgcgtc
      121 ggggtcgccg acgaaccccg cgcgtgggtt cgcgcgggtg atccggccca ccccggtcga
      181 cccgctcacc cggtggtgcc cctcgacgag gtcgagccag ccgaggaacc gctcgaggaa
      241 ccgatggttc agcatcggcc cgtacagcac gtcggcgtac ggatcgccga tcgcggccgc
      301 ctcggtcgcg gcggtgaagc gggcgaggaa gtcgtcgtgg accgactcat ggacgaccac
      361 cgtgccgagc gaggtgcagc gctgcccggc agtcccgaac ccgctgaaca gcgccccctc
      421 gacggccagg tcgaggtcgg cgtccggcat caccacgagg gggttcttcc cgccgagctc
      481 gaggcagggc gactgcaggt gccggccggt gagcgccccg atccggcggc cgacgtccga
      541 cgagccggtg aagccgacct tgtcgacgag cccggccgcg agcgccagct caagcccctc
      601 gaacgcggtc gggccgtccg cgacgaccag gtgcagcacg tccgccggca cgccggcgtg
      661 caggaacagc cgggtgaggg cctcagcgct cgccggcgcg tactcggccg gcttccacac
      721 gaccgggttg ccgcacacca gcgcgggcac gaggtaccag gacgggacgg cgaccgggaa
      781 gttcccggcg gtgacgacgg cggccacccc caccggcacc cggaaggtga acagctgctt
      841 gtcgggcatc tcgctcggta ccgtctggcc gtacagccgc ctcccctcgc caaggaagaa
      901 gctgcacgtg tcgacgacct cctgcacctc gccgagcgac tccgcgtacg gcttgccgat
      961 ctcgtcggtg acgatccggg cgagcgcctc ggcgttgctc tcgacgagcc ggccggcctg
     1021 ctggatggcc cggccgcgga ccggcgccgg cgtggccgcc cacgcgcgcc gggccgcctg
     1081 ggcggcgcgg cacgcctcga ggaacacccc ggcgccggcg agctccgcct cgctcgtcac
     1141 ctggccggtc cgggccgggt tgggcagggc gcgccggcgg gcccctgccg tgccgtgcct
     1201 gggctgcccg ccgatgatcg aggcgacggt tctggtcatg ctgcctccgc tctgcggcgc
     1261 tgccggcgcg gtcgcgccgg ccgggccaac ggtcctcgcg gccatgctgc tcgcccgggc
     1321 cggcagaagg caacgggcgg ccgggcgcgc ggttgtcccc aggcggccgg tgcactccca
     1381 ccccggccct ggcccgtgcg agacttcggt ccatgctcga gctctcacac ctgtcccgtt
     1441 catcacgcca aggcgtgcct ccgagcgcgt gggcccgcgt cctcgacccg cccctcgccg
     1501 ccgccgcggt cgccgcggcg agggaggtgg gcgtgcggct g
//
LOCUS       DASPYY010000044         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1550840, whole genome shotgun sequence.
ACCESSION   DASPYY010000044 DASPYY010000000
VERSION     DASPYY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550840"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VEP73_00565"
     CDS             complement(<1..639)
                     /locus_tag="VEP73_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HYT24964.1"
                     /translation="MTISPDGLKHALDQLAELRTSEATLDEAMRRVVDSTTVLFGVDG
                     TAFMLLDRDQLLRNVAASDPPARTLEELQIEHGEGPAIDAFDEQLVVGATDLAAERRW
                     PGFAPAAVRRGLVAVLASPIPYSEQAVGVLAVLSSVARPWGDTEREAIAAFTDLAALL
                     VLNAMQARERGRLANELQDALDSRIVIEQAKGVLIGRHGLAPRQAFERLRRQS"
     gene            complement(659..901)
                     /locus_tag="VEP73_00570"
     CDS             complement(659..901)
                     /locus_tag="VEP73_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24965.1"
                     /translation="MPDVDPEPTDEQVESRAGSLAAEPGNGRRLRRASRAGDTDVQAR
                     ALLKESEERVEDPATRDPDDPGVIRRTSDEGVLAEE"
     gene            complement(998..>1778)
                     /locus_tag="VEP73_00575"
     CDS             complement(998..>1778)
                     /locus_tag="VEP73_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYT24966.1"
                     /translation="GRGEQIAEEVTRWLRLRRFLHRRLDPRQATGLALTIALAAIFAA
                     GLLFGVVADMITSKTGLARLDASAANWGVRHATKESVRVFDWLTLLGSSAVITVEVVV
                     FGLADRLRRRRWSGAAFLATVALGQNLLANGIKLLFHRERPPVPHLTGATGFSFPSGH
                     SAAAAATYAALALLLGSGRRWPLKVWLGVAAAAVTTAVATSRVLLGVHWLTDVIGGAL
                     LGLGWFAVCSIAFGGPLLHFGAPAERAEAEDRSGVPPPAGD"
BASE COUNT          229 a          637 c          696 g          216 t
ORIGIN      
        1 cgactgcctg cggaggcgct cgaacgcctg cctgggggcg aggccgtggc ggccgatcag
       61 cacccccttg gcctgctcga tgacgatccg ggagtcgagc gcgtcctgca gctcgttggc
      121 gaggcgcccg cgctcgcggg cctgcatcgc gttcaggacg agcagggcgg cgaggtcggt
      181 gaaggcggcg atcgcctcgc gctcggtgtc cccccagggg cgcgcgaccg aggagaggac
      241 cgcgaggacc cccaccgcct gctccgagta cgggatgggg ctcgcgagga ccgcgacgag
      301 cccgcgccgc accgcggcgg gggcgaagcc cggccagcgc cgctccgcgg ccaggtcggt
      361 ggccccgacc accaactgct cgtcgaacgc gtcgatcgcc ggaccctcgc cgtgctcgat
      421 ctgcagctcc tcgagcgtgc gggccggcgg atcggaggcc gcgacgttgc ggaggagctg
      481 gtcgcggtcg agcagcatga acgcggtccc gtcgaccccg aacagcaccg tggtcgagtc
      541 gacgacccgc cgcatggcct cgtcgagggt ggcctcggac gtacgcagct cggcgagctg
      601 gtcgagcgca tgcttgaggc cgtcggggga gatggtcatg agatcctcga gggtcgcgtc
      661 actcctcggc gagcacccct tcgtccgagg ttcgccggat cacgccgggg tcgtccgggt
      721 cgcgcgtggc cgggtcctcg acccgctcct cggactcctt gagcagcgcc cgggcctgca
      781 cgtcggtgtc gcccgcccgg ctggcgcgcc tcagcctcct tccgttgccc ggctcggcgg
      841 ccagcgagcc cgcgcgcgac tcgacctgct cgtcggtggg ctctgggtcc acgtcaggca
      901 tcggcgtcgc ctccgtctct gatcgggtcg gctgctcggg cacgcgggca agcgtacctc
      961 ggtcgtggcc ggcgcgcgcc gcgggtcccc ggtatggtca gtcgcccgcg ggcggcggga
     1021 cgcccgaccg gtcctcggcc tcggcccgct cggcgggcgc gccgaagtgg agcagcggcc
     1081 cgccgaaggc gatcgagcag acggcgaacc agccgagccc gaggagcgcc cccccgatga
     1141 cgtcggtgag ccagtggacg ccgagcagca cccgggaggt ggccacggcg gtcgtgaccg
     1201 cggcggccgc gacgcccagc cataccttga gcggccagcg ccggccgctg ccgagcagca
     1261 gggccagggc ggcgtaggtg gcggcggcgg cggccgagtg gcccgacggg aacgagaacc
     1321 cggtcgcgcc ggtgaggtgg ggcacgggcg ggcgctcgcg gtggaacagc agcttgatcc
     1381 cgttggcgag caggttctgg ccgagggcga cggtcgccag gaacgcggct cccgaccagc
     1441 ggcggcgccg gagccggtcg gcgagcccga agacgaccac ctcgacggtg atcacggccg
     1501 agctgccgag cagggtcagc cagtcgaaga ccctgaccga ctccttcgtg gcgtggcgga
     1561 ccccccagtt ggccgccgag gcgtcgaggc gggcgagccc ggtcttcgag gtgatcatgt
     1621 cggcgaccac cccgaacagc agcccggcgg cgaagatggc ggcgagcgcg atggtgagcg
     1681 cgagaccggt ggcctgcctg gggtcgagcc tgcggtggag gaaccggcgc aggcgcagcc
     1741 agcgggtgac ctcctcggcg atctgctcgc cgcgcccg
//
LOCUS       DASPYY010000045         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_286865, whole genome shotgun sequence.
ACCESSION   DASPYY010000045 DASPYY010000000
VERSION     DASPYY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286865"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VEP73_00580"
     CDS             complement(<1..717)
                     /locus_tag="VEP73_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HYT24967.1"
                     /translation="MAEHLRIGLVYPELLGTYGDRGNAEVLAWRAVRRGVAADVVEVR
                     APAPIPADLDVYLLGGGEDAAQATAADLLRSAAGDGLRQAVLEGRPVLAICGALQLLG
                     RTYADGAGRRTEGLGLVDLHTQSGVPRLVGEVVIRTREGELLTGFENHGGRTRLGRGV
                     EPLGTVVLGAGNNGADGTEGIRQGALVGTYLHGPVLARNPFLADMLLGWVLSRRGVDP
                     GTLRPLGPDPSGRLHDARLRA"
     gene            complement(710..2107)
                     /locus_tag="VEP73_00585"
     CDS             complement(710..2107)
                     /locus_tag="VEP73_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HYT24968.1"
                     /translation="MLVVLEEAPVATDHGRSLNELDYPAATVVPAVQAAIPAQRGHPA
                     GSGRRRAADRLVARGVRLARRVIRLTGRGRGATLPGLLAERCAPGIAGRWAAHLERVV
                     LVSGTNGKTTTTALLSGALAGAGRRVATNASGSNLHRGLVTALLCADQDAQDAVLEVD
                     EAVLPGAIEQLRPVVVALLNLSRDQLDRHHEVGGLGERWRAAMAHLPAGATVVANAGD
                     ARVAHIAEAAPRAVLVGMPGARRGRDDVACPRCGALLVEGPGGAPRCRRCEWAPMPVA
                     VEVWRRHAAVRVRGLGLERRAQLPVGADGYALDAATAWTAATLLGVDPTAAWAAVTAA
                     GTVQGRYAERRWQDVSVRLLLAKNPAGWDEALVAAADRRRAAVVAVNGFGPDGRDTSW
                     LWDVGMARLRDRPRVVATGARAEDVALRLEVAGVGCAIVRPLAAAITGARAEAVDLFA
                     DYTSFQAARRLLGDG"
     gene            2287..2445
                     /locus_tag="VEP73_00590"
                     /pseudo
     CDS             2287..2445
                     /locus_tag="VEP73_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            2448..2648
                     /locus_tag="VEP73_00595"
     CDS             2448..2648
                     /locus_tag="VEP73_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24969.1"
                     /translation="MVRLCREHKLNAISMAMEEQLLAALEREPVALPHPTVAAESSRE
                     AALLIERLAYAALNQTPEARRL"
     gene            2755..>3062
                     /locus_tag="VEP73_00600"
     CDS             2755..>3062
                     /locus_tag="VEP73_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24970.1"
                     /translation="MPSRRPRSRRRSSSRRPRSRPRTASRRSSSLGYLVAVALVAVVG
                     YLLVERGVLPAPPGSTQRPPATRHAPATRGPSGSTTHGPADNSAAIRRLGGTVGYGRG
                     "
BASE COUNT          373 a         1230 c         1125 g          334 t
ORIGIN      
        1 ggcccgcagc cgcgcgtcgt gcagcctccc ggacgggtcc gggccgagcg gacggagcgt
       61 accggggtcg acgccccggc gcgacagcac ccagccgagc agcatgtcgg cgaggaacgg
      121 gttcctggcg aggaccgggc cgtgcaggta cgtgccgacg agcgccccct gcctgatgcc
      181 ctccgtcccg tccgcgccgt tgttgccggc gcccaggacg accgtgccga ggggctccac
      241 gccgcggccg agccgggtgc ggccgccgtg gttctcgaac ccggtgagca gctccccctc
      301 gcgcgtgcgg atcaccacct cccccaccag gcggggcacg cccgactggg tgtggaggtc
      361 gacgagcccg aggccctcgg tgcgccggcc ggcgccgtcg gcgtaggtcc gcccgaggag
      421 ctggagcgcg ccgcagatcg cgagcaccgg ccggccctcg agcacggcct gccggagccc
      481 gtcccccgcc gccgaccgca gcaggtcggc ggctgtcgcc tgggcggcgt cctcgccgcc
      541 cccgagcagg tagacgtcca ggtcggccgg gatgggcgcg ggcgctcgga cctccacgac
      601 gtcggcggcg acgccgcggc ggacggcccg ccaggcgagc acctcggcgt tcccccggtc
      661 gccgtaggtc ccgagcagct cggggtagac gagaccgatc cgcaggtgct cagccatcgc
      721 cgagcaaccg ccgtgccgcc tggaacgagg tgtagtcggc gaacaggtcg acggcctcgg
      781 ccctggcccc ggtgatcgcg gcggcaagcg gccgcacgat cgcgcacccc acgccggcca
      841 cctcgaggcg cagggcgacg tcctcggccc gggcgccggt cgccaccacg cgcggccggt
      901 cgcgcagccg ggccatcccg acgtcccaga gccaggaggt gtcccgcccg tcagggccga
      961 acccgttcac ggcgaccacc gcggcacgcc gccggtcggc cgcggcgacc agcgcctcgt
     1021 cccagccggc cgggttcttg gccaggagca ggcgcacgct cacgtcctgc cagcggcgtt
     1081 cggcgtagcg gccctgcacc gttcccgctg ccgtgaccgc ggcccacgcg gcggtcgggt
     1141 cgacgccgag cagcgtcgcc gccgtccatg cggtggccgc gtccagcgcg tacccgtcgg
     1201 ccccgaccgg gagctgggcg cgccgctcga gcccgaggcc gcggacgcgg acagcggcgt
     1261 gccgccgcca gacctcgacc gccaccggca tcggcgccca ctcgcagcgc cggcaccgtg
     1321 gcgcgccgcc cgggccctcg accaggagcg ctccgcagcg cgggcaggcc acgtcgtcgc
     1381 ggccccggcg ggcgcccggc atgcccacca gcacggcccg aggcgccgcc tcggcgatgt
     1441 gggcgacgcg cgcgtcgccc gcgttggcga cgaccgtcgc gccagctggg aggtgcgcca
     1501 tggcggcgcg ccagcgctcg ccgaggccgc cgacctcgtg gtggcggtcg agctggtcgc
     1561 ggctcaggtt gagcagggcg accacgacgg gccggagctg ctcgatggca ccggggagca
     1621 cggcctcgtc gacctcgagc acggcgtcct gcgcatcctg gtccgcgcac agcagcgcgg
     1681 tgaccaggcc ccggtgcagg ttggagccgg aggcgttcgt ggccacccgc cggccggcgc
     1741 cggccagcgc gccgctgagc agggccgtcg tggtggtctt cccgttggtg cccgagacga
     1801 gcacgacccg ctcgaggtgt gccgcccagc ggccggcgat cccgggagcg caccgctcgg
     1861 cgagcaggcc gggcagggtg gcaccccgcc cgcggccggt cagccggatc acccgccgcg
     1921 ccaggcggac gccacgggcg acgagccggt cggccgcccg gcgcctgccg ctgccggcag
     1981 ggtggccacg ctgggccggg atcgccgcct ggaccgccgg caccaccgtt gccgccgggt
     2041 agtcgagctc gttcagggac cttccatgat ccgttgccac cggcgcctcc tcgagaacga
     2101 cgagcatgcc gccgagcgct tcgtcccgag gtcctgttgt acgtgcttgc cggtgagacg
     2161 ccggtgagac ccgcgtggga gagatatgat ccttgagagg gtcaagatga attgccacaa
     2221 tgcatcggcc gctgtgacgc aggggacgcc atcgccctcg acagctacgc cgaggtcggc
     2281 gccgacgagg gctacgcgct caccaaggcc tggttcctcg aggagcggct gatgatcgcc
     2341 gcccgcacca tcggcgcggc cgagcgggcg ctggagctcg cgacggagtg ggtcgggcag
     2401 cgcgtcacgt tcggccagcc catcaccgag caccagctga tccagcggtg gtccggctct
     2461 gccgcgagca caagctcaac gcgatctcga tggcgatgga ggagcagctc ctcgccgccc
     2521 tcgagcgcga gcccgtcgcg ctgccgcacc cgacggtcgc cgcggagtcc tcgcgcgagg
     2581 ccgcgctgct catcgagcgg ctcgcctacg ccgccctcaa ccagacgccg gaggcgcgcc
     2641 ggctctgacc agcggggctt gcacgcgacc gtccggagcg gcgagcttga gggccacacg
     2701 cgcacgagca ccgcccgcac ccgaccgcct gcacccgacc gccggagccg ccccatgccg
     2761 tcccgtcgcc ccaggtcccg ccgccgttcg agctcccgcc gcccgaggtc ccgccctcgc
     2821 accgcgtccc gccgctccag ctcgctcggc tacctggtcg ccgtggccct ggtcgccgtg
     2881 gtcggctacc tcctcgtcga gcggggggtc ctgcccgcgc cgccgggctc cacccagcgg
     2941 cccccggcga cccggcacgc gccggccacc cggggaccgt cgggctccac cacccatggg
     3001 cccgccgaca acagcgcggc catccgccgc ctgggcggca ccgtcgggta cggccgcggc
     3061 ga
//
LOCUS       DASPYY010000046         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_286889, whole genome shotgun sequence.
ACCESSION   DASPYY010000046 DASPYY010000000
VERSION     DASPYY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VEP73_00605"
     CDS             <1..671
                     /locus_tag="VEP73_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24971.1"
                     /translation="ADWTVDFTGVNDPGLRDMLTVTAALRRIAPEWVAGPDEQFVDHL
                     RGLVINDASGGRSGRKDPRNGAAAGVATRAGAGGGGLGNPFRLARGPWIITFLVGIVI
                     VAFLAAQIASRLGTKANNSSSVSTQGAGTATTSAASGGGGGGVTITTLGTGSTSPSAS
                     VIIPGTTRGTTATTSGGATTSSSATSTTRRATTTSRRATTTSTTTTTTTTTTPTTTTL
                     PPST"
     gene            complement(710..>1637)
                     /locus_tag="VEP73_00610"
     CDS             complement(710..>1637)
                     /locus_tag="VEP73_00610"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HYT24972.1"
                     /translation="RAIPYATPEALRAAVSDRAKDAAAAQPHYSVAERQRQFAYGRLL
                     ARVFSDEPERWVLKGGAALLARLPDARHSRDVDLWGGDEDLDAAERALERSAALDLGD
                     HMSFRVGAWRRGDLNGQPLARTMLICRIGRREFARFGVDLVVGGLPPLEPERVAPPRP
                     VDIPGLPEVPWRLYPIAAQVADKVAGIHKRHEERPSSRYRDLVDLAEIALTQEVAAAD
                     LHLALHSELPRQRVPVPAELAVPDLDRWAAGYAQQARRLPVLDGIGFEQALALVKSLV
                     DPVLAGQRKGTWLPGERRWSDTDAAAEPEPEL"
BASE COUNT          235 a          611 c          587 g          204 t
ORIGIN      
        1 tcgccgactg gaccgtcgac ttcaccggcg tgaacgaccc cgggctgcgc gacatgctga
       61 cggttaccgc cgcgctgcgg cggatcgcgc cggagtgggt cgccgggccg gacgagcagt
      121 tcgtggacca cctccgcggg ctggtcatca acgacgcctc cggcggccgc tcgggacgca
      181 aggacccgcg caacggcgcc gccgccgggg tcgctacacg cgcgggcgcc ggcggcggag
      241 gtcttggcaa cccgttccgc ctcgcccgcg ggccctggat catcaccttc ctggtcggca
      301 tcgtgatcgt cgcgttcctc gccgcccaaa tcgcctcccg gctgggcacc aaggccaaca
      361 attcctcctc ggtcagcacc cagggagcgg ggaccgcgac cacctccgcc gcctcggggg
      421 gaggaggcgg cggcgtcacc attaccaccc tcggcaccgg cagcaccagc ccctccgcga
      481 gcgtcatcat cccaggcacg acccggggca ccacggcgac cacgtccggc ggcgccacca
      541 ccagctcgtc ggcgacctcg acgacacggc gagcgaccac gacgtcgcgg cgggcaacca
      601 ccacctcgac tacgacgacg accaccacga cgaccacccc cactaccacg acgttgccac
      661 cgtcgacgtg agggctggtg cgctcagcca ggcgcgaggt tggcgcccgt tacagctccg
      721 gctccggctc agcagccgcg tccgtgtccg accagcggcg ctcaccaggt agccaggtgc
      781 ccttccgctg accggcgagg accggatcga ccagcgattt cactagggcg agcgcctgct
      841 cgaagccgat gccgtccagg accggaaggc ggcgtgcctg ctgcgcgtag ccggcggccc
      901 agcggtcaag gtcggggacg gctagttcgg cggggaccgg gacccgctgg cgtggcagtt
      961 cgctgtggag ggcgaggtgc aggtcagcgg cggcgacctc ctgggtgagg gcgatctcgg
     1021 cgaggtccac caggtcgcgg taacggctgg agggacgctc ctcgtggcgc ttgtggatgc
     1081 cggcgacctt gtccgccacc tgggcagcga tcgggtatag gcgccagggg acctccggca
     1141 ggcccggtat gtcgaccggt cggggcgggg cgacgcgctc gggttcgagc ggcgggaggc
     1201 cgcccaccac taggtcaacg ccgaagcggg caaactcgcg ccggccgatg cggcagatga
     1261 gcatggtgcg ggcgagcggc tggccgttca ggtcgccgcg ccgccaggcg ccgacgcgga
     1321 agctcatgtg gtcgccgagg tcgagcgcgg ccgaccgctc cagcgcccgc tcggcagcgt
     1381 cgaggtcctc gtcgccaccc cacaggtcga cgtcgcggga gtggcgggca tcgggcagcc
     1441 tggcgagcaa cgccgccccg cccttcagca cccagcgctc cggctcatca ctgaacaccc
     1501 gggcgagcag gcgcccgtag gcgaactggc gctgccgctc ggccacgctg tagtgcggct
     1561 gcgccgcagc ggcgtccttg gcgcggtcgc tgaccgccgc ccgcaacgcc tccggcgtgg
     1621 catacgggat tgctcgc
//
LOCUS       DASPYY010000047         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2533987, whole genome shotgun sequence.
ACCESSION   DASPYY010000047 DASPYY010000000
VERSION     DASPYY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533987"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="VEP73_00615"
     CDS             <1..1642
                     /locus_tag="VEP73_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT24973.1"
                     /translation="VHPGRTPAAQGPRTAAADDRDRIAYEDGGHLWAINADGTGRAQI
                     TRGPGKDAAPRWSPDGRRVAFMRQTRPRRDPTEEVPTVVMVADADGTHQRVVAPLPLG
                     GDFPAWSPDGTRIAFSTRRGLAVVGLDGGPPVRLPVVGSCPAWSPDGTRIAFCGVGVG
                     RPDNSTIMTVGADGSGLRTVLAGTARNFPAGWSPDGSRIVFSSDRDGDLDLYTVGADG
                     VGLTRVLRAPGGQSADAWLPDGRIVYEDTADGSDRSEVRTIDPDGSHLVRYPNLPAPD
                     PIDWLPAAARRARPAVTRPATPAPASQATGRLVDVGGRRLFLDCQGTGPIVVLDAGLG
                     VSSDTWEGVQRGARRFARVCRYDRAGLGASDPGPSPRSSAAMVGDLHTLLHAARVRAP
                     YVLVGASLGGLNAQLFASRYPQEVGGLVLVDALHPALDRRIERILSPAQVRQRRADLA
                     RNPEGVTFADLLDSDAQVRAARIPPDLPLVVLRHGLPFQAPAGFPSAAVERLWAGLQR
                     DLASRRGSLVLARHSHHRIAESEPGLVVDAIRAVAAAG"
     gene            1851..>2302
                     /locus_tag="VEP73_00620"
     CDS             1851..>2302
                     /locus_tag="VEP73_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYT24974.1"
                     /translation="MEENIRLGRIAGIRIGANWSLLAIFVLLVVGLAGGAFPSEYPGR
                     SAAAYLAAAVVAALLFFVSLLAHELAHALVARRDGLEVEGITFWLFGGVSRLSGEART
                     PGSDFRIAAVGPLTSLLLAAVFALAASGLDALGTAALAVAVARWLARI"
BASE COUNT          267 a          916 c          817 g          302 t
ORIGIN      
        1 cgtccatcct ggccggacgc cggccgccca gggaccgcgg acggcggccg cggacgaccg
       61 ggaccggatc gcctacgagg acggcggcca cctctgggcc atcaacgccg atggcaccgg
      121 acgggcccag atcacgcgtg gaccggggaa ggacgccgct cccaggtggt cgccggacgg
      181 ccggcgggtc gcgttcatgc gccagacccg accccggcgc gacccgaccg aggaagtgcc
      241 caccgtcgtg atggtcgccg acgccgacgg gacgcatcag cgggtcgtcg ccccgcttcc
      301 ccttggcggg gacttcccgg cctggtcgcc ggacggcaca cggatcgcct tcagcacccg
      361 ccgcgggctc gcggtcgtcg gtctcgacgg gggcccgccg gtgcggctcc cggtcgtcgg
      421 ctcctgcccc gcctggtcgc cggacggcac gcggatcgcc ttctgcggcg tcggggtggg
      481 ccggcccgac aactccacca tcatgaccgt cggcgccgac ggctcaggcc ttcgcacggt
      541 gctggccggc acggcacgca acttccccgc cggctggtcg cccgacgggt cccggatcgt
      601 gttctcctcc gaccgggacg gcgacctcga cctctacacc gtcggtgccg acggtgtagg
      661 cctgacgcgc gtcctgcgag cgccgggtgg ccagtcggcc gatgcctggc tccccgacgg
      721 ccggatcgtc tacgaggaca ccgcggacgg ctccgaccgc tccgaagtcc gcaccatcga
      781 cccggacggc tcccacctgg tgcgctaccc gaacctcccg gcgcccgacc cgatcgactg
      841 gctccccgcg gccgcacggc gcgcacgccc ggccgtcacc cggcccgcca ctccggctcc
      901 ggcatcccag gcgacgggcc ggctcgtcga cgtcggcgga cggcgcctgt tcctggactg
      961 ccagggcacc ggccccatcg tcgtcctgga cgcgggcctc ggcgtcagct ccgacacgtg
     1021 ggagggggtc caacgcggcg cgcggcggtt cgcccgcgtc tgccgctacg accgcgccgg
     1081 actcggcgcc agcgaccccg gcccgtcccc acgcagcagc gcggcgatgg tcggcgacct
     1141 ccacacgctg ttgcacgccg cccgtgtgcg cgcaccctat gtcctggtcg gcgcgtccct
     1201 cggcggcctc aacgcccagc tgttcgcgag ccgctaccca caggaggtcg gcggcctcgt
     1261 cctcgtcgac gcgctccatc ccgccctgga ccgccgcatc gagcggatcc tgtctcctgc
     1321 ccaggtcagg cagcggcgcg ccgacctggc ccgcaacccg gagggggtga cgttcgcgga
     1381 cctgctcgac agcgacgccc aggtccgcgc cgcccggatc ccgcccgacc tcccgctcgt
     1441 cgtcctccgc cacgggctgc cgttccaggc ccccgccggc ttcccgagcg cggcggtcga
     1501 gcgcctctgg gccggactcc agcgcgacct cgccagccgg cgcggctccc tcgtcctggc
     1561 gcggcacagc caccaccgca tcgccgagtc cgagcccggc ctggtcgtcg acgccatccg
     1621 cgccgtggcg gcggccggct gacccggccc cacggcacga gggacccggt cggccgcgcg
     1681 gcgccgccga ggatccgtgc cggggatccg tgcttgacat cggctcgagg tggtagcaag
     1741 aggtggtagc aagagaaggt gtggcgcgtc ggttcgagcg ggacgatgac gcgaagctga
     1801 ccaccagagg acgcggcggc gatctggccg cggtaacgga cggaccaggc atggaagaga
     1861 acattcgact cggccggatc gccggcatcc gcatcggcgc gaactggagc ctgctggcca
     1921 tcttcgtgct gctcgtggtc gggctggccg gcggcgcgtt cccgagcgag tacccgggcc
     1981 ggagcgcggc cgcgtacctg gcggccgcgg tcgtcgcggc cctgctcttc ttcgtgtcgc
     2041 tgctggccca cgagctggcc cacgcgctgg tcgcccgccg ggacggtctg gaggtcgagg
     2101 gcatcacctt ctggctgttc ggcggcgtct cgcgtctctc cggcgaggcg cgcacgccag
     2161 gcagcgactt ccgcatcgcc gccgtcgggc cgctgaccag cctcctgctc gccgcagtgt
     2221 tcgccctggc ggcctcgggc ctggacgcgc tcggcacggc ggcgctcgcc gtcgcggtgg
     2281 cgcggtggct cgcaaggatc aa
//
LOCUS       DASPYY010000048         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_146859, whole genome shotgun sequence.
ACCESSION   DASPYY010000048 DASPYY010000000
VERSION     DASPYY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146859"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VEP73_00625"
     CDS             complement(<1..348)
                     /locus_tag="VEP73_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HYT24975.1"
                     /translation="MSPPASYRGLPVSEGVAVGEIHLADAATAAHATPEQVRAAFAAV
                     AADRSALADRLRAAGRDEAADIVGVGALIAADPALVEPAAAAAGEGTEAGAAVHRAAA
                     AQAAVLAALSTPEL"
     gene            complement(345..980)
                     /gene="dhaL"
                     /locus_tag="VEP73_00630"
     CDS             complement(345..980)
                     /gene="dhaL"
                     /locus_tag="VEP73_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HYT24976.1"
                     /translation="MDAEFFRAWIAEATRAIENQRDHLTQLDAAIGDADHGINLTRGF
                     GAVATALQQADPKTPGSVLMLTGSTLISKVGGASGPLYGMAFRQAGKALGDAPEVEMH
                     ALAGALDAALAGVQKLGAAREGDKTMVDALAPAASALAKALAEGAAAADALSASAGAA
                     AAGAEATIPMQALKGRASYLGPRSVGHEDPGAASTALILGALRDVAARQGR"
     gene            complement(1020..>1516)
                     /locus_tag="VEP73_00635"
     CDS             complement(1020..>1516)
                     /locus_tag="VEP73_00635"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HYT24977.1"
                     /translation="GADLGRVVATAAEVNERSRSFGVALTSCTVPAAGKPTFELGDAE
                     IELGIGIHGEPGRERATLATAREITAAALDAIDADMPLTGDLLVMVNGMGGTPLIELY
                     LVYNEVHRYLTGRGARVARNLVGNYVTSLEMQGFSVTVCRLTATLTELWDAPVDTPAL
                     RWGR"
BASE COUNT          182 a          572 c          545 g          217 t
ORIGIN      
        1 aagttccggc gtgctcagcg ccgctagcac cgcggcctgc gccgccgcgg cgcggtgcac
       61 cgccgccccg gcctcggtgc cctcacccgc cgccgctgcc gcgggctcga cgagggcggg
      121 atcggcggcg atcagggcac cgaccccgac tatgtcggcg gcctcgtccc gcccggcggc
      181 gcgcagccgg tccgcgagtg cgctgcggtc ggccgccacc gcggcgaaag ccgcccgcac
      241 ctgctcgggc gtcgcgtgag ccgcggtggc cgcatcggcg aggtgaatct cgccgaccgc
      301 cacgccctcg gaaacgggca gcccgcggta gctggcgggg gggctcatcg gccctgccgc
      361 gcggcgacat cgcgcagggc accgaggatc agggcggtgg acgccgcgcc cgggtcctcg
      421 tggccgacgc tgcgcggccc gaggtagctc gccctgccct tgagtgcctg catcgggatc
      481 gtggcctcgg ctccggccgc ggcggcgccg gcgctggcgg agagggcgtc ggccgcggcc
      541 gccccctccg ccagcgcctt ggcgagagcg ctcgccgccg gggcaagcgc gtcgaccatc
      601 gtcttgtcac cctcccgcgc cgcgcccagt ttctggacgc cggccaaagc cgcgtcaagc
      661 gcgcccgcca gggcgtgcat ctccacctcg ggcgcatccc ccagcgcctt ccctgcctgg
      721 cggaaggcca tgccgtacag cgggcccgac gcgccgccga ccttcgagat cagggtcgac
      781 ccggtcagca tgaggacgga gcccggtgtc ttcgggtccg cctgctgcag cgcggtggcg
      841 accgcgccga agccgcgggt caggttgatg ccgtgatcgg cgtcgccgat cgccgcgtcg
      901 agctgggtga ggtggtcacg ctggttttcg atggctctgg tggcctcggc gatccacgcg
      961 cggaagaatt cggcgtccat gagggctcct agcgatccgg gaaagacggt cctggatcct
     1021 taccgtcccc aacgcagtgc cggtgtgtcg acgggcgcgt cccacagctc tgtcagcgtg
     1081 gcggtaagcc ggcacacggt caccgaaaat ccctgcatct ccaggcttgt gacgtagttg
     1141 cccacgaggt ttctcgcgac ccgggcgccc ctgccggtca ggtacctgtg gacctcgttg
     1201 tagaccaggt agagctcgat cagcggtgtt ccgcccatgc cgttgaccat caccagcagg
     1261 tcgccggtca gcggcatgtc ggcgtcgatg gcgtcgagcg ccgcggccgt gatctcgcgt
     1321 gcggtcgcca gcgtcgcgcg ctccctgccc ggctcgccgt ggatgccgat gccaagctcg
     1381 atctcggcgt cgccgagctc gaaggtcggc ttgcccgcgg cgggcacggt gcacgaggtc
     1441 agcgcgacac cgaacgagcg gctgcgctcg ttgacctccg ccgccgtcgc caccacccgg
     1501 ccgaggtcgg ccccgc
//
LOCUS       DASPYY010000049         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_147501, whole genome shotgun sequence.
ACCESSION   DASPYY010000049 DASPYY010000000
VERSION     DASPYY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147501"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VEP73_00640"
     CDS             complement(<1..263)
                     /locus_tag="VEP73_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYT24978.1"
                     /translation="MSETIAFLVRHGYALLFVWLLLEQLGLPLPAIPVLLAAGALAGA
                     GRMSLALALAVALAGALLGDAVWYLLGRRRGAKVLNLLCRMSL"
     gene            complement(277..>1717)
                     /locus_tag="VEP73_00645"
     CDS             complement(277..>1717)
                     /locus_tag="VEP73_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT24979.1"
                     /translation="LVALLQAAATLPLFLLAVPSGALADVVDRRRLLIFAQIWMTAAA
                     ALLGVLTVAGAVTPSILLGATFLLGLGAAVNAPAWQAIIPELVSGQELAAALSLQGAG
                     INLARAVGPALGGLVVAAAGPGAAFLLNAVSFLGVVVVLYAWRREHHPSVLPAERILG
                     AMSAGLRYVRHAPDLRAVMIRSSTFIVFGSALWALLPLVVRFELGLGPTGYGLLLGCL
                     GAGAVAGAVLVPWLRRRLAVGTLVAGASVVFALALLGMAFIVQAWAMGVVMFAAGVAW
                     LALLTSFHTAAQTVLPGWVRGRGLSIYLLVLFGGMAAGSALWGAVAKVVGPSAALASA
                     ALGILAGLAATVRFSLSSGEGLNLTPSRHWPAPVVSGTPVDDDGPVMVTVEYHVEPRR
                     AHDFARAMRDVRRIRLRDGAIRWNLLNDSADPSRYVESFLVESWVEHLRQHERVTVAD
                     REIEARARAFHIGASPPVVSHFIARALPR"
BASE COUNT          291 a          637 c          640 g          149 t
ORIGIN      
        1 tcgagcgaca tcctgcagag gaggttcagg accttcgcgc cccgccgccg tccgaggagg
       61 taccagacgg cgtcgccgag gagcgcgccc gcgagggcga cggcgagcgc cagcgccagg
      121 ctcatgcggc cggcgcccgc gagcgccccg gccgccagca gcaccgggat cgccggaagc
      181 ggcaggccca gctgctcgag cagcagccac acgaacagga gcgcgtagcc gtgccggacc
      241 aggaacgcga tcgtctcgct catgccgggc cctcgctcac cggggcagcg cccgggcgat
      301 gaagtgcgat acgaccgggg gcgaggcgcc gatgtggaag gcgcgggccc gggcctcgat
      361 ctcgcgatcc gcgacggtga cccgctcgtg ctgccgcagg tgctcgaccc acgactcgac
      421 caggaacgac tcgacatagc gcgacggatc ggccgagtcg ttcagcaggt tccagcggat
      481 ggcgccgtcg cgcagcctga tccggcgcac gtcccgcatg gcgcgggcga agtcgtgcgc
      541 gcgccgcggc tcgacgtggt actcgacggt gaccatgacc gggccgtcgt cgtccaccgg
      601 cgttcccgac acgacggggg ccggccagtg cctcgacggg gtcaggttca gcccctcgcc
      661 ggacgacagg ctgaagcgca ccgtggcggc gaggccggcg aggatcccga gcgccgcgga
      721 cgctagcgcc gcgctcggcc ccacgacctt ggccacggcg ccccagagcg cgctgccggc
      781 ggccatgccg ccgaagagaa ccaggagata gatcgacaac ccgcgcccgc gcacccaccc
      841 ggggaggacc gtctgcgccg cggtgtggaa gctcgtcaag agcgccagcc aggcgacccc
      901 ggcggcgaac atcaccactc ccatggccca ggcctggacg atgaacgcca tcccgaggag
      961 cgccagggcg aagaccacac tggcccccgc caccagcgtc ccgaccgcga gcctgcgccg
     1021 cagccacggc acgaggaccg ccccggccac ggcgccggcg ccgaggcagc cgagcagcag
     1081 accgtagccg gtcggcccga gcccgagctc gaagcgcacg accaagggca ggagggccca
     1141 gagcgcgctg ccgaagacga tgaaggtgct cgagcggatc atcacggcgc gcaggtcggg
     1201 agcgtgccgg acgtagcgca gccccgcgga catggcaccg aggatccgct cggccggcag
     1261 gacgctcgga tggtgctccc gcctccaggc gtagaggacc acgacgacgc cgaggaacga
     1321 gacggcgttc aggaggaagg ccgcccccgg ccccgccgcg gcgaccacga ggccgcccag
     1381 cgccgggccg acggcgcgcg ccaggttgat cccggcgccc tggagggaga gcgccgccgc
     1441 cagctcctgg cccgagacca gctcggggat gatcgcctgc caggcgggcg cgttcaccgc
     1501 ggcgccgagg ccgagcagga aggtcgcccc cagcaggatc gatggggtga cggcaccggc
     1561 gacggtcagc acgccgagca gcgcggccgc cgccgtcatc cagatctgcg cgaagatgag
     1621 caggcgccgc cgatcgacga cgtcggcgag ggcaccggat ggcaccgcca ggaggaagag
     1681 cggcagcgtc gccgcggcct gcagcagggc gacgagg
//
LOCUS       DASPYY010000050         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_428757, whole genome shotgun sequence.
ACCESSION   DASPYY010000050 DASPYY010000000
VERSION     DASPYY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428757"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VEP73_00650"
     CDS             <1..585
                     /locus_tag="VEP73_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HYT24980.1"
                     /translation="VVVFTAFRQTLGFLTELAAAAGLPAVAYHGGLTRREKEGAIRSF
                     REELGILLTTEAAGEGRNLQFCHVMVNFDLPWNPMQIEQRLGRIHRIGQDHEVLLTNL
                     VTRGTVEERILKVLEAKINLFELVVGELDMILGRIEEDFDFEHFVFDAHLASRDDAEF
                     QARLDALGDDLVRARRDHLDSRERTDRLIAPSEP"
     gene            582..>1741
                     /locus_tag="VEP73_00655"
     CDS             582..>1741
                     /locus_tag="VEP73_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24981.1"
                     /translation="MTATDPAVQFWLRYVEAEGGLHEDTGDKVLMMLPGALQAALDLP
                     EDVAVTADPEVAREDGALLLLPGHPVLDHAATHVLDQGDGGFAWLAWPRAAPPTPPLL
                     LERARERFDVDHGRIDPEGHPAQVYLPVLRVGALVSYAAGLGEHFQEREEVWVDARNG
                     LVLREGLARAVASMPRLEVPDAPHQALVPDLAAALAGAHAGIEDRALARREALARQAQ
                     GPLREELARSDAYYEAALAAIAKRRGQAPPDRRRLLDAQAEATRVEQARRQAEIAEKF
                     RPSHQARPFRLHLLLVPALELPVQVRRGAAAHPFRFTWILPRAGFVDVRCPRCGAADQ
                     LVAGRDRLGCRACLGRPTAAAAPSPAPAPPPAGASAEAAPSRPAPRPPPAPTR"
BASE COUNT          216 a          664 c          640 g          221 t
ORIGIN      
        1 gtggtggtgt tcaccgcctt ccgtcagacg ctcggcttcc tcaccgagct cgccgccgcg
       61 gcgggcctgc ccgcggtcgc ctaccacggg ggcctgaccc ggcgggagaa ggagggggcg
      121 atccgctcct tccgcgagga gctggggatc ctgctgacca ccgaggcggc tggcgagggc
      181 cgaaatctcc agttctgtca cgtcatggtc aacttcgacc tgccctggaa cccgatgcag
      241 atcgagcagc ggctcggtcg catccatcgc atcggccagg accacgaggt gctgctcacc
      301 aacctggtca cccgtgggac cgtggaggag cggatcctca aggtcctcga ggccaagatc
      361 aacctgttcg agctggtcgt cggcgagctg gacatgatcc tcgggcgcat cgaggaggac
      421 ttcgacttcg agcatttcgt gttcgacgcc cacctcgcca gccgcgacga cgcggagttc
      481 caggcgcggc tggacgcgct cggtgacgac ctggtccgag cgcgccgtga ccacctggac
      541 agccgggagc gtaccgaccg gctcatcgcc ccgagcgagc catgaccgcc accgaccccg
      601 ccgtccagtt ctggctccgc tacgtcgagg cggagggcgg cctgcacgag gacaccggcg
      661 acaaggtcct gatgatgctc cccggcgcgc tgcaggccgc gctcgacctt cccgaggacg
      721 tcgcggtcac cgccgacccg gaggtggcgc gcgaggacgg ggcgctgctg ctgctcccgg
      781 gccaccccgt gctcgaccac gcggcgacgc acgtgctcga ccagggtgac gggggcttcg
      841 cgtggctggc ctggccaagg gcggcacccc cgacgccgcc gctgctgctg gagcgggcac
      901 gggagcggtt cgacgtggac cacggccgca tcgaccccga gggccacccg gcccaggtgt
      961 acctgccggt gctgcgggtg ggcgcgctgg tctcctacgc ggccgggctg ggtgagcact
     1021 tccaggagcg cgaggaggtc tgggtcgacg cgcgcaacgg cctggtgctc cgcgagggcc
     1081 tggcgcgggc ggtcgcgtcg atgcctcgcc tggaggtccc cgacgcgccg caccaggcgc
     1141 tcgtgcccga cctggccgcg gccctcgccg gggcgcatgc gggcatcgag gaccgggcgc
     1201 tggcgcgccg ggaggcgctg gcgaggcagg cacagggccc cctgcgtgag gagctggccc
     1261 ggtccgacgc ctactacgag gccgccttgg cggccatcgc caagcggcgg ggccaggcgc
     1321 cacccgaccg ccggcggctc ctggacgccc aggccgaggc gaccagggtc gagcaggccc
     1381 gccggcaggc cgagatcgcg gagaagttcc gcccgagcca ccaggcccgg ccgttccggc
     1441 tgcatctgct gctggtgccc gcgctcgagc tgcccgtgca ggtccgccgc ggcgcggcgg
     1501 cgcacccgtt caggttcacc tggatcctgc cccgcgcggg cttcgtcgac gtccgctgcc
     1561 cccgctgcgg cgccgccgac cagctggtgg cgggtcggga ccggctcggc tgccgtgcct
     1621 gcctcgggag gcccaccgcg gccgccgccc cctccccggc gccggcgcca ccacccgccg
     1681 gggcgtccgc ggaggcggcg ccttcgaggc ccgcaccccg gccgccaccc gcgcccaccc
     1741 g
//
LOCUS       DASPYY010000051         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_288296, whole genome shotgun sequence.
ACCESSION   DASPYY010000051 DASPYY010000000
VERSION     DASPYY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288296"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VEP73_00660"
     CDS             <1..1266
                     /locus_tag="VEP73_00660"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYT24982.1"
                     /translation="AAAAGAEVVVSLNASPYRRSKGERLEPMLATRAADHGTHLFYVN
                     LLGGQDELVFDGQSASFDPQGRTQARAGAFTEELLIVDLTLEEGATARLSDRPRRSPA
                     VDPHPALAVRRVQVTDALPDPGPPAERRLAAATGTEEEVYTALVTATRDYVRKNGFES
                     VLVGLSGGIDSSLVAAVAADALGRDRVTGVAMPSSYSSEGSVTDARALAGSLGIRFLV
                     LPVAEPVEAFLSVLKEPFAGTEEGVAEENLQSRSRGTLLMALSNKFGSLVLATGNKSE
                     MAVGYATLYGDMAGGFAVIRDVPKTLVYDLCRWRNQAGAVIPQSVLDKPPSAELRPGQ
                     LDTASLPPYEVLDPILEAYIEEDASVDELVARGFDRETVEKVVKLVDGAEYKRRQAPP
                     GPKITGRSFGKDRRLPITNRFRGVPRPAR"
     gene            1380..>2054
                     /gene="panB"
                     /locus_tag="VEP73_00665"
     CDS             1380..>2054
                     /gene="panB"
                     /locus_tag="VEP73_00665"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYT24983.1"
                     /translation="MPVAVADLAAWKARGERFVMLTAYDALTARVLDEAGVPLLLVGD
                     SVGNNVLGYETTIPVTMRESLVFTAAVARGASNALVVGDLPFGAYQASAAEAVRNAAR
                     LVKAGANAVKLEGGRRSSKAVRRMTRAGIPVMGHLGFTPQSLNAFGGFRVQGRGEKGD
                     ELLADALALQEAGCFAIVLEMVPSELGARLTEALDVATIGIGAGPGVDGQVLVITDLI
                     GLTGDPA"
BASE COUNT          281 a          773 c          743 g          257 t
ORIGIN      
        1 gccgccgcgg ccggggccga ggtcgtggtc agcctgaacg cgtcgccgta ccgccgctcc
       61 aagggcgagc ggctggagcc gatgctcgcc acccgagccg ccgaccacgg cacccacctg
      121 ttctacgtga acctgctcgg aggccaggac gagctggtct tcgacggcca gagcgcctcc
      181 ttcgacccgc agggccgcac ccaggcccgg gccggcgcct tcaccgagga gctgctgatc
      241 gtcgacctca ccctggagga gggggcgacc gcacgcctgt cggaccggcc ccgccggtcc
      301 cccgccgtcg acccccaccc ggccctcgcc gtgcgccggg tccaggtcac cgacgccctg
      361 cccgaccccg gcccgccggc cgagcgccgg ctcgccgccg cgaccggcac ggaggaggag
      421 gtctacaccg cgctcgtcac ggcgacacgg gactacgtgc ggaagaacgg cttcgagtcg
      481 gtgctcgtcg ggctgtccgg cgggatcgac tcgagcctgg tcgcggcggt cgcggccgac
      541 gcgctcggcc gcgaccgggt caccggcgtc gccatgccgt cctcgtactc gtcggagggg
      601 agcgtgaccg acgcccgcgc cctggcgggc agcctcggca tccggttcct cgtcctgccg
      661 gtcgcggagc cggtcgaggc gttcctgtcg gtgctcaagg agccgttcgc cggcaccgag
      721 gagggggtcg ccgaggagaa cctccagtcg cggagccgcg gcaccctgct catggcgctg
      781 tccaacaagt tcggcagcct cgtgctcgcc accggcaaca agtccgagat ggcggtcggc
      841 tacgccacgc tgtacggcga catggcgggc ggcttcgccg tgatccggga cgtcccgaag
      901 acactcgtgt acgacctctg ccgctggcgc aaccaggccg gggcggtgat cccccagtcg
      961 gtgctcgaca agccgccgtc ggccgagctg cggccgggcc agctcgacac cgcctccctc
     1021 cctccctacg aggtcctcga cccgatcctc gaggcctaca tcgaggagga cgcgagcgtc
     1081 gacgagctcg tcgccagggg cttcgaccgc gagacggtcg agaaggtcgt gaagctggtc
     1141 gacggcgccg agtacaagcg gcgccaggcg cccccgggac cgaagatcac cggacggtcg
     1201 ttcggcaagg accgccggct gccgatcacc aaccggttcc ggggggtccc ccgcccggcc
     1261 aggtagcgcc cggacgccgg cccgcgccgc acgggccggc gtcgaccggg gcatggcaca
     1321 cttgagagcg tccaggggac cctcgcgggc tccaaggcac acatagcaag gaggccgcca
     1381 tgccggtcgc cgtcgccgac ctcgccgcct ggaaggcgcg cggggaacgg ttcgtcatgc
     1441 tcaccgccta cgacgcgctg accgcgcgcg tgctcgacga ggcaggggtc ccgctcctgc
     1501 tcgtcggcga ctcggtcggc aacaacgtcc tcggctacga gaccaccatc ccggtcacca
     1561 tgcgggagtc gctggtgttc accgcggcgg tggcccgcgg ggcaagcaac gcgctggtcg
     1621 tcggggacct gccgttcggg gcctaccagg cctcggcggc cgaggcggtg cgcaacgccg
     1681 cccggctcgt caaggccggg gcgaacgcgg tcaagctcga gggcggccgc cggtcgagca
     1741 aggcggtccg caggatgacc cgggccggga tcccggtcat gggccacctc ggcttcacac
     1801 cccagtcgct caacgccttc ggcggcttcc gggtccaggg ccgcggcgag aagggcgacg
     1861 agctgctggc cgacgcgctc gcgctccagg aggccggctg cttcgcgatc gtgctggaga
     1921 tggtcccctc cgagctcggc gccaggctca ccgaggcgct cgacgtcgcg acgatcggga
     1981 tcggggcggg gccgggggtc gacggccagg tgctcgtgat cacggacctg atcggcctca
     2041 ccggggatcc cgcc
//
LOCUS       DASPYY010000052         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_7851, whole genome shotgun sequence.
ACCESSION   DASPYY010000052 DASPYY010000000
VERSION     DASPYY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7851"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VEP73_00670"
     CDS             <1..397
                     /locus_tag="VEP73_00670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYT24984.1"
                     /translation="ALRVTSFDGQTVTLDGFRGRPALVSFFESWCPICKVEQPDLSRV
                     AREFAGRVGFVGVSYHDTVAGGRAYQRRFGVSYPLANDASGRTWARWHVPYQPVTVLV
                     DKRGRIAERFDGGTTGGTLSAALAYLAAE"
     gene            456..914
                     /locus_tag="VEP73_00675"
     CDS             456..914
                     /locus_tag="VEP73_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24985.1"
                     /translation="MEGEQDGRGLQVEQLRQALLSARARLESERLTAVAVDRAASEET
                     GSARPRRAWRLVVPAATLAVGMVLGFALGSARAGREPAGAAAARPPTTRPAPPTSVVV
                     RPSASPACLETATRGDRLIELLVTNQRDKVADLLVAYTVASRQCRRDASP"
     gene            1051..1515
                     /locus_tag="VEP73_00680"
     CDS             1051..1515
                     /locus_tag="VEP73_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24986.1"
                     /translation="MRRAIGIGAVVIAILAAVGIGVGAYNLGVSRGLEHGGHAVEAVR
                     VVGPGFHHGYGYGYFPFGLILFPLFLLGIFLLLRGAFWRGRWGGPGYGPGGWGGPGAR
                     SWGGPGPWGQGGPAAGERAEEWHRRQHGDQAGERQREQAGEQPDGGGEPAGT"
BASE COUNT          190 a          582 c          658 g          205 t
ORIGIN      
        1 tgccctgcgg gtgacctcct tcgacggcca gacggtcacc ctggacggct tccgcggccg
       61 gccggcgctg gtgagcttct tcgagtcctg gtgcccgatc tgcaaggtcg agcagccgga
      121 cctgtcacgg gtggccaggg agttcgccgg ccgggtcggc ttcgtcggcg tctcctacca
      181 cgacacggtg gccggcggcc gcgcctacca gcgccgcttc ggcgtctcct acccgctcgc
      241 caacgacgcg tccgggcgga cgtgggcccg ctggcacgtg ccgtaccagc cggtcacggt
      301 gctggtcgac aagcggggcc ggatcgccga gcgcttcgac ggcgggacca cgggcggcac
      361 gctctccgcc gccctcgcct acctggccgc cgagtgacgg gagggcgggt tcggaggtcg
      421 cctggtcgcg ggcaccgacc accagaggag catgcatgga gggggagcag gacggacgag
      481 gtctccaggt ggagcagctg cggcaggccc tcctttcggc gcgggcacgg ctcgagtccg
      541 agcggctgac ggcggtggcg gtcgaccggg cagcctccga ggagaccggc agcgcccgcc
      601 cgcggcgggc gtggcgcctg gtcgtcccgg cggcgaccct cgcggtcggg atggtgctcg
      661 ggttcgccct ggggtccgca cgggcgggcc gggagccggc gggcgcggcg gcggcgcgcc
      721 cgcccacgac ccggccggcc cccccgacgt cggtcgtggt gcggccgtcg gcctccccgg
      781 cctgcctgga gaccgcgaca aggggcgacc ggctcatcga gctgctggtc accaaccagc
      841 gcgacaaggt cgccgacctg ctcgtggcct acaccgtggc gagccggcag tgccgcaggg
      901 acgcctcccc gtaggccgcg ggcagcgccc gcgcggcctc ggcgggcggg ccctcccgca
      961 ggcctcccca ccgcgttcac aggtccttca caccaccacg gcagcatggg ccccgtgaga
     1021 tgaggaaccc cgtcaaccaa ggaggacgag atgcgacggg cgatcggtat cggggcggtg
     1081 gtgatcgcga tcctggccgc ggtcgggatc ggggtcggcg cctacaacct gggggtgtcc
     1141 cgggggctcg agcacggcgg ccacgccgtc gaggcggtgc gggtggtcgg cccgggcttc
     1201 caccacgggt atgggtacgg ctacttcccg ttcgggctga tcctgttccc gctgttcctg
     1261 ctcgggatct tcctgctgct gcgcggcgcc ttctggcgcg ggcgctgggg cggcccgggc
     1321 tacggccctg gtggctgggg cggcccgggc gcaaggtcct ggggcggccc aggcccctgg
     1381 gggcagggcg gcccggcggc cggggagcgg gccgaggagt ggcaccgccg ccagcacggc
     1441 gaccaggcgg gcgagcgcca gcgcgagcag gcgggcgagc agccggatgg cgggggcgag
     1501 ccggccggca cgtgaccgcg cccgacccga ggcggggcgg aggcttgcct ccgccccgcc
     1561 gtctcgtcag gcttcgggag ggatcggagg cagaccgtga agctgatcct ggtggtcgag
     1621 gacgagacga agatc
//
LOCUS       DASPYY010000053         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1412835, whole genome shotgun sequence.
ACCESSION   DASPYY010000053 DASPYY010000000
VERSION     DASPYY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412835"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..941)
                     /locus_tag="VEP73_00685"
     CDS             complement(297..941)
                     /locus_tag="VEP73_00685"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HYT24987.1"
                     /translation="MYQRDYILRQVELAAQALARILRLARDGRGEEAVGLFDQAYRPL
                     VGVSGRVVATLDEEQLLTLLTSGSSPDPRRVAVAVELMVAEGDLHAEAGREADAAIRY
                     GRALGLAAYLASHMGRLPDRGLAARLAARAGELDLTAAQRLHLCRLQEALGRYADAED
                     SLFEAIDGAPDPTAAIDQGIAFYQRLLARDDDDLEAGGLPRDEVRAALVDLLHR"
     gene            complement(1039..1140)
                     /locus_tag="VEP73_00690"
     CDS             complement(1039..1140)
                     /locus_tag="VEP73_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HYT24988.1"
                     /translation="MRTKAAVVAFQRSHGLESDGIVGPLTWRARFSS"
     gene            complement(1159..2307)
                     /locus_tag="VEP73_00695"
     CDS             complement(1159..2307)
                     /locus_tag="VEP73_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HYT24989.1"
                     /translation="MTEQASQRTTPAPTVGDWVDLVHHRWPAATAEPWDNVGLQVGDP
                     ATRATTVLVALDVTAAVLDEAAGLGAGLVVAHHPLVFRPLASVRAGDPVGGLVLRAAV
                     RGTAVLAAHTNFDVAAEGSPSDVVADLLELADRRPLRPAAGEPAADYKYVVFCPTEAT
                     MKVLDAAARAGAGIIGNYTRCSFRTPGTGTFQPGEGARPALGEVARLNQVAEERLEVV
                     VPADRLAAVRAAVAAAHPYEEVAADVYPLHAELPGLGRVGSLPLPATLSSLAERLREG
                     LPAPGLRVAGDPDRRVRRVAVLGGSGGDEVAAAVAAGAECYVTGDLKHHQVLAALAAG
                     VACIDATHAATEQAALPGLAERLRADAAAAGWAVDVRLSTVPPHPFSG"
     gene            complement(2685..>3063)
                     /locus_tag="VEP73_00700"
     CDS             complement(2685..>3063)
                     /locus_tag="VEP73_00700"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HYT24990.1"
                     /translation="GVLGEAIVRRLADSGWHGPLARTGGGRWHLYLPPSLVGTTLVRP
                     AERNPARQLYVHGTGGYVVAPPSRHVTGVAGRWIRPLRAQVASLPVALGSLLADPPGP
                     APGAARPAFDRSTGGIATGRVAG"
BASE COUNT          379 a         1150 c         1194 g          340 t
ORIGIN      
        1 tcgcgctggc ggtgatcggc aagccgttgg cgtcgcagaa ccgggccagg agccggacgg
       61 cctcggcgta ggcctggacg gtccgctcgc tcttgttctc cgcgcgcagt gtgcgggcga
      121 agctggcgtt cagggcggtg agatcgcccg gcgggggagc agtgcccata cagcatagtt
      181 agatcggctt cgagcgcagt gcaagcggca gtcatgcgcc gctgcatgcc gtgcatggca
      241 tctgagctgc ctgaatgctg acaaccgcgc cggctggcgg gtgtctcctg gccgacctac
      301 cggtgcagca ggtcgaccag ggcggcccgc acctcgtcgc gggggaggcc gccggcctcg
      361 aggtcgtcgt cgtcccgcgc cagcaggcgc tggtagaagg cgatgccctg gtcgatcgcg
      421 gcggtcgggt caggggcgcc gtcgatcgcc tcgaacagcg agtcctcagc gtccgcgtag
      481 cgcccgagcg cctcctgcag ccggcacagg tgcaggcgtt gcgcggcggt gaggtcgagc
      541 tccccggccc gcgccgcgag cctcgcggcc agcccgcggt cgggcaggcg ccccatgtgg
      601 ctggccaggt acgcagccag cccgagcgcg cgcccgtagc ggatggcggc gtccgcctcc
      661 cgtcccgcct cggcgtgcag gtccccctcg gccaccatca gctcgacggc cacggccacc
      721 cgccgggggt cggggctcga ccccgacgtg agcagggtca ggagctgctc ctcgtccagg
      781 gtcgcgacca cccgcccgct cacgccgacg agcggccggt acgcctgatc gaacagcccg
      841 accgcctcct cgccgcgtcc gtccctggcg aggcgcagga tgcgggcgag cgcctgcgcc
      901 gccagctcga cctgccgcag gatgtaatcg cgttggtaca gcggacctcc ctgccggcca
      961 gcggggaccc ggcacgggtc cccgcgccct ccgacgagga gggaccgtgg ctgccaagcc
     1021 tacagcccgc cggcgcggtc aggacgagaa ccgcgcacgc caggtcagtg gcccgacgat
     1081 gccgtcggat tcgaggccgt gggaccgctg gaacgcgacc acggccgcct tggtccgcag
     1141 cccgaagcgc cggaccggtc agcccgagaa ggggtggggc gggaccgtgg acaggcggac
     1201 gtcgaccgcc cagccggcgg cggccgcgtc ggcccggagc cgctcggcca ggccgggcag
     1261 ggcggcctgc tcggtggccg catgggtggc gtcgatgcag gccacgcccg cggccaaggc
     1321 cgcaagcacc tgatggtgct tcaggtcgcc ggtcacgtag cactcggctc ccgccgccac
     1381 cgcggccgcc acctcgtcgc cgcccgaccc gccgagcacg gcgacccgtc tcacccgccg
     1441 gtcagggtcg ccggccaccc ggagaccggg cgccggcaga ccctcccgca gccgctcggc
     1501 cagcgaggac agggtcgccg gcagcggcag gctgccgacc cggccgaggc cggggagctc
     1561 ggcgtgcaac gggtagacat cggccgccac ctcctcgtag ggatgggcgg cggcgacggc
     1621 ggccctgacg gccgccagcc ggtcggccgg cacgacgacc tccaggcgct cctctgccac
     1681 ctggttgagc cgcgcgacct cgccgagggc cgggcgggca ccctctccgg gctggaacgt
     1741 gcccgtgccc ggggtgcgga acgagcaccg cgtgtagttg ccgatgatcc cggcgccggc
     1801 ccgcgcggcg gcatcgagca ccttcatggt cgcctcggtc gggcagaaga cgacgtactt
     1861 gtagtcggcg gccggctcgc cagcggcagg gcggagaggc cgccggtcgg cgagctcgag
     1921 caggtcggcg accacgtccg acggcgagcc ctccgcggcc acgtcgaagt tggtgtgggc
     1981 ggccagcacc gcggtgcccc gcacggccgc ccgcagcacg aggccgccca ccgggtcgcc
     2041 ggcccggacc gacgcgagcg gccggaacac gagcgggtgg tgggcgacga cgagcccggc
     2101 gccgagcccg gcagcctcgt cgaggacggc cgcggtcacg tcgagcgcta ccagcacggt
     2161 ggtggcccgg gtggccgggt cgcccacctg cagcccgacg ttgtcccagg gctcggcggt
     2221 ggccgccggc cagcggtggt ggacgaggtc gacccagtcg ccgacggtcg gcgccggggt
     2281 ggtgcgctgg gatgcctgct cggtcatgtc gcatcgtctc cggggttcgg gtgccgagga
     2341 cggtcggcgc gtcccgagac ggaacgaagg ctgcgggggc acgccgacgg actccggccg
     2401 tgcccccgcg agccggccgg tgacccggtg gggaccccgc cgtgacctct cagggaatca
     2461 tgcaacacga gggcggtccg atgctcaccc ggaggacagg cggcgcggct ggcgcgcctg
     2521 cgccgcagcg ggggtcgccg ctccctaccg ctgcggcgca ggcggcttct cgcccgtcgg
     2581 ggccggcgcc ccggcccacg ggcccggcgg ctgcgcgccg ccggcgtccg gcgatcctgc
     2641 gatttcctcg tcgtccgggg gcaccggaat gggatggcag ccggtcaccc cgccacccgc
     2701 ccggtggcga tgccgcctgt cgaccggtcg aacgccgggc gggccgcgcc aggcgccggg
     2761 cccggcgggt cggcgagcag cgacccgagg gcgacgggga ggctcgcgac ctgcgcccgc
     2821 aggggccgga tccagcggcc ggcgacgccg gtgacgtgcc ggctcggggg ggcgaccacg
     2881 tacccgcccg tgccgtggac gtagagctga cgggcggggt tgcgctcggc cggccggacc
     2941 agggtcgtcc cgaccagcga cggcggcagg tacaggtgcc agcggccgcc gcccgtccgg
     3001 gcgagcgggc cgtgccagcc agagtccgcg aggcggcgga cgatcgcctc gccgagcacc
     3061 ccg
//
LOCUS       DASPYY010000054         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2676912, whole genome shotgun sequence.
ACCESSION   DASPYY010000054 DASPYY010000000
VERSION     DASPYY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676912"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VEP73_00705"
     CDS             complement(<1..1202)
                     /locus_tag="VEP73_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYT24991.1"
                     /translation="MIAKLLVANRGEIARRVMRTCRAMGIATVAVFADPDAGEPFAVE
                     ADEAVPIGGATPAESYLRAEAILDAAERTGADAVHPGYGFLAENPDFARGCRKRDLVF
                     VGPPAEAIAAMGDKLEAKRLAAAAGIPVLPGADATALTSGELPAAADRVGWPVLVKAA
                     LGGGGVGMRVVRDRGDLAGAVAAAARQAAAAFGDGTVLLERYVEAPRHVEVQVLGDAH
                     GRVVHLFERECSIQRRHQKLVEEAPSPAVDARLRRRLGAAAVAAAEAVGYVGAGTVEF
                     LLAPDGEFWFLEMNTRLQVEHPVTEAVTGLDLVRLQLLVAEGAPLPREALDARVTGHA
                     IEARLYAEDPLRGFLPATGTLHRFRIDPRPGLRVDAGVADGSTVGIHYDPMLAKVIAY
                     APTRREAAG"
     gene            complement(1199..2797)
                     /locus_tag="VEP73_00710"
     CDS             complement(1199..2797)
                     /locus_tag="VEP73_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYT24992.1"
                     /translation="MGVLSSRVDPTADAYLANRDANLELLRELDAELARSRAGGGERY
                     VARHRARGKLPARERIELLLDRDAAFLELSPLAAWGTDFHVGASVVTGVGPVSGVECV
                     VVASDPTVRGGATNPFSLRKTLRAMEIAERNRLPLLQLIESGGADLPTQAEIFIPGGA
                     VFRNLTRLSRAGIPTVALVFGNSTAGGAYVPGMSDHTVMVRGRARVFLGGPPLVRMAT
                     GEESGEEELGGAEMHARTSGLADLLAADELDCLRLGRQVVANLNWRKLGPAPRLPAEA
                     PRYDAEDLLGLAPVDLKVPADVREILARLVDGSRFDEHKPLYGPSLVTGWARIHGWPV
                     GILANSRGVLFSEESNKAAQFVQLANQVDTPLVFLQNTTGYMVGREYEQRGIVKDGAK
                     MINAVSNSTVPHLTVILGASYGAGGYGMAGRAYDPRFLFAWPASKSAVMGPAQLAGVM
                     SILNRASATSAGRPFDEEADRAMRQGVEDHIEAESRALFNTGLLYDDGVIDPRDTRTV
                     LGIALSACHSGEVSGTDGFGVFRM"
     gene            complement(2935..>3250)
                     /locus_tag="VEP73_00715"
     CDS             complement(2935..>3250)
                     /locus_tag="VEP73_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYT24993.1"
                     /translation="ADVILVSPVSCWEVATLLCSGRIALDRDVYVWVRDLFASDEVEP
                     APLTPQVAVSAALLATAGFHGDPADRMLYATALEQSVPLVTKDGSIRSYAKKAGDLRT
                     IW"
BASE COUNT          435 a         1207 c         1193 g          415 t
ORIGIN      
        1 ccggccgcct cgcggcgcgt gggggcgtag gcgatcacct tggcgagcat cgggtcgtag
       61 tggatcccga ccgtcgaccc gtcggcgacc ccggcgtcga cgcgcaggcc gggccggggg
      121 tcgatgcgga agcggtgcag ggtgccagtg gcgggcagga agccgcgcag cgggtcctcg
      181 gcgtagaggc gcgcctcgat cgcgtggcca gtcacgcgcg cgtcgagcgc ctcgcggggc
      241 agcggcgccc cctcggcgac gagcagctgg aggcggacga ggtccaggcc ggtgaccgcc
      301 tcggtgaccg ggtgctcgac ctggagccgg gtgttcatct cgaggaacca gaactcgccg
      361 tccggagcca gcaggaactc caccgtgccg gcgccgacgt acccgaccgc ctcggcggcg
      421 gcgaccgccg ccgcgccgag gcggcgccgc agccgcgcgt ccaccgccgg cgacggcgcc
      481 tcctcgacga gcttctggtg ccgccgctgg atcgagcact cccgctcgaa caggtggacc
      541 acccggccgt gggcgtcgcc caggacctgg acctcgacgt gcctcggcgc ctcgacgtac
      601 cgctcgagga gcacggtgcc gtcgccgaac gccgcggcgg cctgccgcgc cgcggcggcc
      661 accgcgccgg ccaggtcccc gcggtcgcgg acgacccgca tgccgacccc gccgccgccg
      721 agcgccgcct tgaccaggac gggccagccg acccggtcgg ccgcggcggg cagctcgccg
      781 gaggtgagcg cggtggcgtc ggcaccgggg agcaccggga taccggccgc ggccgcgagc
      841 cgcttggcct cgagcttgtc gcccatggcc gcgatcgcct cggccggcgg cccgacgaac
      901 acgaggtcgc gcttgcggca gccgcgggcg aagtcggggt tctcggccag gaagccgtag
      961 ccagggtgga ccgcgtcggc gccggtccgc tcggccgcgt ccaggatcgc ctccgcgcgc
     1021 aggtacgact cggcgggggt cgcgcccccg atggggaccg cctcgtcggc ctcgacggcg
     1081 aacggctcgc ccgcgtcggg gtcggcgaac accgccaccg tcgcgatccc catcgcccgg
     1141 cacgtccgca tgacccggcg ggcgatctcg cctctgttgg cgaccagcag cttcgcgatc
     1201 acatccggaa cacgccgaac ccgtccgtgc cgctcacctc ccccgagtgg caggcggaga
     1261 gcgcgatccc gaggacggtg cgggtgtcgc gcgggtcgat caccccgtcg tcgtagagca
     1321 gcccggtgtt gaacagcgcc ctcgactccg cctcgatgtg gtcctccacc ccctggcgca
     1381 tggcgcggtc ggcctcctcg tcgaaggggc ggccggccga ggtggccgac gcccggttga
     1441 ggatcgacat gaccccggcg agctgggccg ggcccatgac cgccgacttc gaggccggcc
     1501 aggcgaacag gaagcggggg tcgtacgccc gcccggccat gccgtacccg ccggcgccat
     1561 aggacgcgcc gaggatgacc gtgaggtgcg gcaccgtgct gttcgagacc gcgttgatca
     1621 tcttggcgcc gtccttgacg atgccgcgct gctcgtactc cctgcccacc atgtagccgg
     1681 tggtgttctg caggaacacg agcggggtgt cgacctggtt ggcgagctgg acgaactggg
     1741 ccgccttgtt cgactcctcg ctgaacagca cgccccgcga gttcgcgagg atcccgaccg
     1801 gccagccgtg gatccgcgcc cagccggtga cgaggctcgg gccgtagagc ggcttgtgct
     1861 cgtcgaagcg ggagccgtcg accaggcggg cgaggatctc gcggacgtcg gcgggcacct
     1921 tgaggtcgac cggggcgagc cccaggaggt cctcggcgtc gtaccgcggc gcctcggccg
     1981 gcaggcgcgg ggccggcccg agcttgcgcc agttgaggtt ggcgacgacc tgcctgccga
     2041 ggcgcaggca gtcgagctcg tcggcggcca gcaggtcggc gagccctgag gtgcgcgcgt
     2101 gcatctcggc gccgcccagc tcctcctcgc cggactcctc gccggtggcc atcctgacga
     2161 gcggcgggcc cccgaggaac acccttgccc tgcccctcac catcacggtg tgatcggaca
     2221 tgccgggcac gtaggcgccc ccggcggtcg aattaccgaa caccagcgcc actgtgggga
     2281 tgccggcgcg ggacaggcgc gtgaggttgc ggaatacggc tccgccggga atgaaaatct
     2341 ccgcctgggt cggcaggtcg gcaccgcccg actcgatgag ctgcagcagc gggagccggt
     2401 tgcgctcggc gatctccatc gcccgcagcg tcttgcgcag gctgaaggga ttggtggcgc
     2461 cgccgcgcac ggtcgggtcg ctcgcgacga cgacgcactc cacccccgag accgggccga
     2521 caccggtgac cacgctcgcg ccgacgtgga agtcggtccc ccacgccgcg agcggcgaca
     2581 gctcgaggaa cgcggcatcc cggtcgagca gcagctcgat ccgctccctg gcgggcagct
     2641 tgccgcgcgc gcggtgccgg gcgacgtacc gctcgccccc gccagcccgg ctcctcgcca
     2701 gctcggcgtc gagctcgcgc agcagctcga ggttggcgtc ccggttggcc aggtaggcgt
     2761 cggcggtcgg gtcgacccgg ctggagagca cgcccatacg cccgtcgcct ctcttcggcc
     2821 cgcgtccgca cgcctgccac aagcagctaa gcatgccctg gccagaggtt tcaacggtgg
     2881 gcgggcgctg gcgggaccac ccgacctcgt caggcgtcac ccgcccgtcc cgagctacca
     2941 gatcgtgcgg agatcgcccg ccttcttggc ataggaccgg atcgacccgt ccttggtgac
     3001 gagcgggacc gactgttcca gcgcggttgc gtacagcatg cgatcggcag ggtcgccatg
     3061 gaatcctgcc gtggcgagca gcgcggcgct gaccgccacc tgtggggtca gcggggcagg
     3121 ttccacctcg tccgaggcga acaggtcccg gacccagacg tacacgtccc ggtcgagcgc
     3181 gatccgcccg ctgcagagca gggtcgcgac ctcccagcac gagacggggc tcacgaggat
     3241 cacgtcggcc
//
LOCUS       DASPYY010000055         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1272791, whole genome shotgun sequence.
ACCESSION   DASPYY010000055 DASPYY010000000
VERSION     DASPYY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272791"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2137)
                     /gene="ftsH"
                     /locus_tag="VEP73_00720"
     CDS             complement(170..2137)
                     /gene="ftsH"
                     /locus_tag="VEP73_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYT24994.1"
                     /translation="MPQQHHRTQPVRHQSNQVKSPPPDRPPRPAPPPPPKWRAWLPAL
                     GLILTLLLLFLPSRMSQGQVEQLSYTNLKAKVAAGEVKSVEIGSDGHIEGQLKNGTRF
                     RSALPTALNDPELPKLLDGKGVEVKAVGPSTSAGSVLLSLLPLLLFVAFFVYLGRSTR
                     RQLAGLGGIGRSRGKVYDMEERPSTTFADVAGYEGAKREVSEVVDFLKNPDRYRKAGA
                     VGPKGVLLVGPPGTGKTLLARAVAGEAGVPFISVTGSSFVKMFVGVGAARVRDLFNDA
                     RKRAPSIIFIDELDAIGQRRGGQVISNDERDQTLNQMLAEMDGFDPATGVVVMGATNR
                     PEVLDPALLRPGRFDRQVVIPLPTQRERRAILEVHVRGKKLGPDVDLDVVARGTPGFS
                     GADLANLVNEAAIFAVRAGREVIAAVDFSEARDRILLGRRDSTNALLPDEKRAVAVHE
                     SGHALVAAISEHGDPVAKVTILPAGQALGVTEQLPIDERHLYSESYLKDSLAIRMGGR
                     AAEKMVFGETSTGAANDLASATDLAMRMVREFGMSPALGPVGFASGSPMYLGGEEVRS
                     RPYAEATQRVIDEEVEKFLREADERASDLLVEHRDALDRLTLLLLDRETVDGTDVDAV
                     LGRVPGQAEPVGATGHAATATSSKEPGAPAR"
     gene            complement(2232..2777)
                     /locus_tag="VEP73_00725"
     CDS             complement(2232..2777)
                     /locus_tag="VEP73_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HYT24995.1"
                     /translation="MAEVFESSDIPGVWLVRLQPVDDARGRFMETYRASWLPFAAAMV
                     QGNRSESAPKVLRGLHYHRRQADFWFVQSGQVTTVLLDTRIGSPTEGKHATLRQGGGQ
                     DVAVYIPEGVAHGFHAHDQVVMTYLVTNEYDSTDELGVAWDDPDLGIAWPEREPVLSE
                     RDRKNPRLSDVPADRRVPYSG"
     gene            2991..3473
                     /locus_tag="VEP73_00730"
     CDS             2991..3473
                     /locus_tag="VEP73_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYT24996.1"
                     /translation="MAEATGAAGLELLLFRLAGRGYACRRETVREVVDIGTGQPPVPG
                     MPSSSLGALGADDRPVAVVSLRIALGLPDLGPVGRIVVVETRLGPVGFLVDDVLRVER
                     LDPGTSRIHPRSLAGGPRSLAGGYVSATVETPGGSFLLVDWDAVPLPGRPGDTPGAPR
                     "
     gene            complement(3521..3811)
                     /locus_tag="VEP73_00735"
     CDS             complement(3521..3811)
                     /locus_tag="VEP73_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT24997.1"
                     /translation="MLLTGYVLALVALAVQLALAEIVVRRPGAARRAVPYAAIALSAA
                     GTAAADALGRSAEFTPGLRGLQVACFGVALAGALVVVRAVTGVLAAEKRRGR"
BASE COUNT          495 a         1444 c         1541 g          568 t
ORIGIN      
        1 cgccgagccg cgcgagcacg gcgtacggcc cgagccgccg ggggtcggtg tgctggagcg
       61 ggctggtcat cgtcatcgtt ctcgccgcct ggcgggcggc ctcctcccgg gatcggccgc
      121 tccagtgacg cacaggagcc cgggaggaga caatggggcc gcgggcacgt cagcgggcgg
      181 gcgctcccgg ctccttggag ctcgtggcgg tcgccgcgtg cccggtggcg ccgaccggct
      241 cggcctggcc ggggacccgg ccgaggaccg cgtcgacgtc ggtgccgtcg accgtctccc
      301 ggtcgagcag cagcagggtc aggcggtcga gggcgtcgcg gtgctcgacc aggaggtcgc
      361 tggcccgttc gtcggcctcg cgcaggaact tctccacctc ctcgtcgatc acccgctggg
      421 tcgcctcggc gtagggccgg ctgcgcacct cctcgccgcc gaggtacatc gggctcccgg
      481 aggcgaagcc gaccgggccg agcgccgggc tcatgccgaa ctcgcggacc atgcgcatgg
      541 ccaggtcggt cgctgaggcg aggtcgttgg cggccccggt cgaggtctcg ccgaacacca
      601 tcttctcggc cgcccggcca cccatgcgga tcgcgaggga gtccttgagg tagctctcgg
      661 agtacaggtg gcgctcgtcg atcgggagct gctcggtcac ccccagggcc tgcccggcgg
      721 gcaggatggt gaccttggcg acggggtcgc cgtgctcgga gatcgccgcg accagggcat
      781 ggccggactc gtggacggcg accgcgcgct tctcgtcggg cagcagcgcg ttggtgctgt
      841 cgcggcggcc gagcaggatg cggtcgcggg cctcggagaa gtcgaccgcg gcgatcacct
      901 cgcggccggc ccggaccgcg aagatggccg cctcgttgac caggttggcg aggtccgcgc
      961 cggagaagcc gggggtgccg cgggcgacca cgtccaggtc gacgtcgggg ccgagcttct
     1021 tcccgcggac gtgcacctcg aggatggccc tgcgctcccg ctgggtgggc agggggatga
     1081 cgacctggcg gtcgaagcgg ccggggcgga gcagggccgg gtcgagcacc tcgggccggt
     1141 tggtggcgcc catcaccacg accccggtgg ccgggtcgaa gccgtccatc tcggcgagca
     1201 tctggttcag ggtctggtcc cgctcgtcgt tggagatcac ctgcccgccg cggcgctggc
     1261 cgatggcgtc gagctcgtcg atgaagatga tcgagggcgc ccgcttgcgg gcgtcgttga
     1321 acaggtcgcg gacccgggcc gcgccgacgc cgacgaacat cttgacgaag ctcgagccgg
     1381 tgaccgagat gaacggcacc ccggcctcgc cggccaccgc ccgcgccagc agcgtcttgc
     1441 ccgtccctgg ggggcctacc aggaggacgc ccttcggccc gacggcgcct gccttgcggt
     1501 accggtcggg attcttcagg aagtcgacga cctcgctgac ctcccgcttg gcgccctcgt
     1561 agccggcgac gtcggcgaag gtggtgctcg gccgctcctc catgtcgtag accttgccgc
     1621 gggagcgtcc gatgccgccg agcccggcga gctggcgcct ggtggagcgg cccaggtaga
     1681 cgaagaacgc cacgaacagc agcagcggca ggaggctcag gagcaccgag ccggccgagg
     1741 tgctcggccc caccgccttg acctcgacgc ccttcccgtc gagcagcttg gggagctccg
     1801 ggtcgttcag ggcggtcggc agggcggagc ggaagcgggt gccgttcttg agctgcccct
     1861 cgatgtggcc gtcggagccg atctcgaccg acttgacctc gcccgcggcg accttggcct
     1921 tcaggttggt gtagctgagc tgctccacct ggccctggct catccgcgac ggcaggaaga
     1981 ggaggagcag ggtgaggatg aggccgaggg cgggcagcca cgcccgccac ttgggcggcg
     2041 gcggcggtgc gggccttggc ggccggtcgg gcggcggcga cttcacttgg ttgctctgat
     2101 gcctcactgg ttgcgtccgg tggtgctgct gcggcatggc gcgctcctga agggcgagct
     2161 gacgtctccc tacggtagcg ccgcaggagg gtaacgcggg agggtgacgt gtagggagag
     2221 cgtgtacctc gtcagcccga gtacggaacg cggcgatcgg cggggacgtc ggaaaggcgc
     2281 gggttcttgc ggtcccgctc ggagaggacc ggctcgcgct cgggccaggc gatccccagg
     2341 tcggggtcgt cccatgccac cccgagctcg tcggtgctgt cgtactcgtt cgtcaccagg
     2401 tacgtcatga ccacctggtc gtgggcgtgg aagccgtggg cgacgccctc cgggatgtag
     2461 acggcgacgt cctgcccgcc gccctgccgg agggtcgcgt gcttgccttc ggtcggtgag
     2521 ccgatgcggg tgtcgagcag cacggtcgtg acctgcccgg actgcacgaa ccagaagtcg
     2581 gcctggcgcc ggtggtagtg caggccgcgt agcaccttgg gtgccgactc cgagcggttg
     2641 ccctgcacca tcgcggcggc gaacggcagc caggaggccc ggtaggtctc catgaagcgg
     2701 ccccgtgcgt cgtcgaccgg ctgcagccgg acgagccaga ccccggggat gtcgctggac
     2761 tcgaagacct cggccacgcg gcgctccctt cctctcctcg aggagttcca ggacggggga
     2821 gggtacctgc ccccggctcc ccacgctact tctggctgca atgtgatccc tgtaggtggc
     2881 cgctggagct gccgaggtag taccgcgggc ctacgcccga tggccgcttc cccgaggaga
     2941 gctcatgccc gtccccaccg ccggccgcct cgcgacctcc gggccagccg ttggctgagg
     3001 cgaccggtgc ggcgggcctc gagctgctcc tgttccggct ggccgggcgg gggtacgcct
     3061 gccgtcgcga gaccgtccgg gaggtcgtcg acatcggcac cgggcagccg ccggtgcccg
     3121 ggatgccgtc gtcgagcctt ggcgcgctcg gcgccgacga ccgcccggtg gccgtggtca
     3181 gcctgaggat cgccctcggc ctgcccgacc tcgggccggt cgggcgcatc gtcgtcgtcg
     3241 agacccggct cggcccggtc ggcttcctcg tcgacgacgt gctgcgcgtc gaacggctcg
     3301 accccgggac cagccggatc catccgagga gcctcgccgg cggcccgagg agcctcgccg
     3361 gcggctacgt cagcgcgacg gtggagaccc cgggcgggtc cttcctgctg gtcgactggg
     3421 acgccgtccc gctgccgggc cgcccgggcg acacgcctgg cgcgccccgc tagccctccc
     3481 gccggccggc gctcggcgag ctggccaccc tggctcgctg tcagcgaccg cgccgcttct
     3541 cggccgcgag cacgccggtg accgcgcgca cgaccaccag cgcgccggcc agcgccaccc
     3601 cgaagcaggc gacctggagc ccgcgaagcc cgggcgtgaa ctcggcgctg cgcccgaggg
     3661 cgtccgcggc tgccgtcccg gccgcgctca gggcgatcgc ggcgtagggg accgcgcgcc
     3721 gggccgcccc ggggcggcgg acgacgatct cggccagcgc gagctggacc gcgagggcca
     3781 cgagggcaag gacgtatccg gtgagcagca aggacgatca cctcatcgca tctcgatcgc
     3841 aggtcgcgtc cgcacgtgtg ccaggtgcgc ggactggtcc ggtcgccgcc ggacggtcgc
     3901 ggccgagcgg cctcgagacc attctggtcg tgtcggcggc gagcgcaacc ccctacgggg
     3961 tgggcccgcg acccgccggt cccggtcggc ggcgaccgcc cgggcggccc ggcgcgcggt
     4021 cccctacgcc gcgatcgccc tgagcgcg
//
LOCUS       DASPYY010000056         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2256338, whole genome shotgun sequence.
ACCESSION   DASPYY010000056 DASPYY010000000
VERSION     DASPYY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256338"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VEP73_00740"
     CDS             complement(<1..1032)
                     /locus_tag="VEP73_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYT24998.1"
                     /translation="MARFRLLNLRHVVRQRARSVLSVAGIAVAAALIVTVFSTYGSLT
                     GSVQRLGARVAGNAEIEVAGVTDTGLDQRLLVGVRTTSGVDAAVPLIRSPVLLGGRQA
                     VLFGVDNSLSALGGEFERLVTSASATAFKGPPGVFVGDALAHAAKLRVGSDLALLSGS
                     GTPSSVKVAGVLGGPQARRINAGFFAIAPLPLAQQLLHKPGQVDSILVVVDPRVGAQE
                     VGRRLDQVVGGRALVSTPQLRTEQAAAATRVFQDATLFVALMTLAVGGLLVFDLMSMI
                     GLERRKELAVLRALGATRPMLLRDFLAEAALLGVAGAALGCPAGVLLGRWLVVRLPPF
                     LVASFDTRLQ"
     gene            complement(1011..1754)
                     /locus_tag="VEP73_00745"
     CDS             complement(1011..1754)
                     /locus_tag="VEP73_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT24999.1"
                     /translation="MPRVQLDDVTRSYARGQHVVRALDHVSLTIEQGEFVAVVGPSGA
                     GKSTLLHLVGGLDAPSSGTVRIGGDDLARLSDTARAAFRRTRLGFVFQFFNLLPTMSA
                     WENVALPKVLDGTRLAHARGDAERLLERVGLAHRATHRPSELSGGELQRVALARALVM
                     DPALILADEPTGNLDSEAGKEILDLLASCALDDGRTVVVVTHNPAAAARARRVVTLSD
                     GRVASDQTTGGQTSSDQTTRGQAWHASAS"
     gene            complement(1727..>2033)
                     /locus_tag="VEP73_00750"
     CDS             complement(1727..>2033)
                     /locus_tag="VEP73_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25000.1"
                     /translation="DRHQVGYALPAGGIYLFVADADGGAEAEAVRALGAAGVRVLPGD
                     AFGASGRGWLRTTFAVPDDELVGGFTRVGQVLATQGMQRNRTTQRRRPPCRVSSSTT"
BASE COUNT          294 a          701 c          798 g          240 t
ORIGIN      
        1 ctgcagcctg gtgtcgaagg aggcgaccag gaacggcggc agccgcacca ccagccagcg
       61 accgagcagg accccggcag gacagccgag ggcggcgccg gccacgccga gcagggcggc
      121 ctcggcgagg aagtcgcgca gcagcatcgg gcgggtggcg ccgagggcgc gcaggaccgc
      181 cagctccttg cggcgctcca ggccgatcat gctcataagg tcgaagacga gcaggccgcc
      241 gacggcgagg gtcatcaggg cgacgaacag ggtggcgtcc tggaacacgc gggtcgcggc
      301 agctgcctgc tccgtgcgga gctggggcgt gctgaccagg gcgcgcccgc cgaccacctg
      361 gtcgagccgc cggccgacct cctgggcgcc gacccgcggg tcgaccacga ccaggatcga
      421 gtcgacctgg cctggcttgt ggagcagctg ctgcgcgagc gggagcggag cgatggcgaa
      481 gaacccggcg ttgatgcgcc gcgcctgggg tccgccgagc acgccggcga ccttgacgga
      541 gctgggcgtc ccggagcccg acagcagggc gaggtcgctg ccgacccgca gcttggcggc
      601 atgggcgagg gcgtcgccga cgaaaacgcc tggcggaccc ttgaaggcgg tggcgctggc
      661 gctggtcacc agccgctcga actcgccgcc gagcgcgctg aggctgttgt cgacgccgaa
      721 cagcaccgcc tgccggccgc cgagcaggac gggcgagcgg atcagcggca cggcggcgtc
      781 gacccctgag gtggtccgga cgccgaccag cagccgctgg tcgagcccgg tgtcggtaac
      841 gccagcgact tcgatctcgg cgttgccggc gacccgggcg ccgaggcgtt gcaccgagcc
      901 ggtcagtgag ccgtaggtgg agaagacggt gacgatcagc gcggcggcga cggcgatgcc
      961 ggcgaccgac agcaccgaac gcgcccgctg ccggacgacg tggcgcaggt tcaggaggcg
     1021 gaagcgtgcc atgcctgccc cctggtggtc tggtcgctgg aggtctgtcc cccggtggtc
     1081 tggtcgctgg cgacgcggcc gtcgctgagc gtgacgacac gccgcgctct ggccgcggcg
     1141 gcggggttgt gggtcacgac cacgaccgtg cggccgtcgt cgagggcgca gcttgccagc
     1201 aagtcgagga tctccttgcc cgcctcggag tcgaggttgc cggtgggctc gtcagcgagg
     1261 atcagcgcgg ggtccatcac cagcgcacga gccagcgcga cccgctgcag ctcaccgcct
     1321 gacagctccg acgggcggtg ggtggcgcgg tgggcgagcc cgacccgctc cagcaggcgc
     1381 tcggcgtcgc cgcgggcgtg cgccaggcgg gtaccgtcga ggaccttggg cagggcgacg
     1441 ttctcccacg ccgacatggt cgggagcagg ttgaagaact ggaacacgaa gccgagccgg
     1501 gtgcgccgga aggcggcgcg agcggtgtcg gagaggcgcg cgaggtcgtc gccgccgatg
     1561 cggaccgtcc cggagctggg ggcgtccaag ccgccgacga ggtgcagcaa ggtgctcttg
     1621 cccgcgccgc tggggccgac caccgccacg aactcgccct gctcgatggt gagcgagacg
     1681 tggtcgagcg cgcgcacgac gtgctggccg cgggcatagg agcgggtcac gtcgtcgagc
     1741 tggacacgcg gcatggcggt cttctcctct gggttgtccg gttccgctgc attccttggg
     1801 tggcgagcac ctggccgacg cgagtgaacc cgccgaccag ctcgtcgtcg ggcacggcga
     1861 aggtggtccg gagccagcca cgcccgctcg caccgaaggc gtcgcctggg agcacacgca
     1921 cccccgccgc gccgagcgcg cggacggcct ccgcctccgc gccgccgtcg gcgtccgcga
     1981 cgaacaggta gatcccgccg gccggcagcg cgtagccgac ctggtggcgg tcg
//
LOCUS       DASPYY010000057         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1132870, whole genome shotgun sequence.
ACCESSION   DASPYY010000057 DASPYY010000000
VERSION     DASPYY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132870"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VEP73_00755"
     CDS             <1..403
                     /locus_tag="VEP73_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT25001.1"
                     /translation="ANLAAMAAGLPYTIAAQWALPDRQCIAWVGSEGFAKLMAEFLTA
                     ARHRLPVKVVVSNTPPPRPPHQAGTSPPATADFALWARACGGLGVRVERSDEVEAAVR
                     AALDHPGPALVDVVIDPDELPEPVGGELDPG"
     gene            551..1534
                     /locus_tag="VEP73_00760"
     CDS             551..1534
                     /locus_tag="VEP73_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT25002.1"
                     /translation="MGTPKVVVVTGATSGVGRATTRAFASGGASVGLVARGADALRAA
                     AKEAEAAGGRGLVLPADVADAGQVEEAATRAEAELGPVDVWVNNAMTSVFAPFAEIEP
                     DEFRRVTEVTYLGVVNGTRAALRRMVPRDRGTIVQVGSALAYRGIPLQSAYCGAKHAI
                     QGFTESVRCELFHDRSHVRIAMVQLPALNTPQFEVVLSRLPQKSQPVPPVYQPEVAAR
                     AIVWAAGHSRREVWAGASTAATIVANSVVPGLLDRYLARTGFRSQQTGEPADPDRPAN
                     LWEPVPGDRGAHGAFDPRSHGQSAQLWATTHRGLLAAAVGLAGAAVGLSRR"
     gene            complement(1586..>2293)
                     /locus_tag="VEP73_00765"
     CDS             complement(1586..>2293)
                     /locus_tag="VEP73_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYT25003.1"
                     /translation="EEFEPEVGATVVRVLERNGVAVTYPRQNCCGLPLVSNGDFDSAR
                     GYAGKLVRRLSPSASDPGVIIANSTSCGMTLKAKYRELLGMEDERTTAVSRAVYDICE
                     YLLELHDDGELDTGLGEIRARAVYHPPCQLMAHGIGTPALDLLALVPGLEVERSRAGC
                     CGTAGTYGTKVEKYEIAMDVGQTLFDQVADVRPEFVVCDSETCRWHIARATGLPVYHP
                     VQVLDHAYALADSRRRG"
BASE COUNT          296 a          847 c          862 g          288 t
ORIGIN      
        1 ggccaacctg gcggcgatgg cggccggcct gccgtacacc atcgccgcgc aatgggcgct
       61 cccggaccgg cagtgcatcg cctgggtcgg ctcggagggg ttcgcgaagc tcatggccga
      121 gttcctcacc gccgccaggc accggctgcc ggtcaaggtc gtggtcagca acaccccgcc
      181 gccgcgcccg ccccaccaag ccggcacgtc gccgccggca acggcggact tcgccctctg
      241 ggcacgggcg tgcggcgggc tcggcgtccg cgtcgagcgg tcggacgagg tcgaggcggc
      301 ggtccgcgcc gccctcgacc atcccggccc cgcgctggtc gacgtggtga tcgaccccga
      361 cgagctgccc gagccagtcg gtggtgagct cgaccccggg tgaccggcgc ggcggggtcc
      421 cgcagcagca tgaggacagg tgaccggtgg gtgaggggag ccggcagcgg gtagacggcc
      481 ttgtcccgcc cacggacgcc ggaccccgcc gccacgggca gcggcccgga tcgcagcaag
      541 gagagcgcac atggggactc ccaaggtcgt cgtcgtcacc ggcgccacgt cgggcgtggg
      601 gcgggcgacg acgcgagcat tcgcgagcgg cggcgcgagc gtcggcctgg tcgcccgggg
      661 cgccgacgcc ctacgggcgg cggccaagga ggcggaggcg gccggcgggc gcgggctcgt
      721 cctgcctgcc gacgtcgccg acgccggaca ggtcgaggag gcggccaccc gtgccgaggc
      781 cgagctcggc ccggtcgacg tctgggtcaa caacgccatg acctcggtgt tcgcgccgtt
      841 cgccgagatc gagcctgacg agttccgccg ggtgacggag gtcacctatc tcggcgtggt
      901 caacggcacg agagcggccc tgcgccgcat ggtgccccgc gaccggggca ccatcgtgca
      961 ggtcggctcc gccctggcgt accgggggat cccgctgcag agcgcctact gcggggcgaa
     1021 gcacgccatc cagggattca ccgagtcggt gcgctgcgag ctcttccacg accggtccca
     1081 cgtacgcatc gccatggtgc agctgcccgc gctgaacacg ccgcagttcg aggtcgtgct
     1141 ctcacggctg ccccagaagt cccagcccgt cccgcctgtc taccagcccg aggtcgccgc
     1201 ccgcgcgatc gtgtgggcgg ccggccattc ccgtcgggag gtgtgggccg gcgccagcac
     1261 ggccgccacg atcgtggcca acagcgtggt gccggggctg ctcgaccggt acctggcccg
     1321 gaccggcttc cggtcgcagc agaccggcga gccggccgac cccgaccggc cggccaacct
     1381 gtgggagccg gtgccagggg accgcggggc gcacggcgcg ttcgaccccc gctcgcacgg
     1441 acagagcgcg cagctctggg ccaccaccca ccgcggcctc ctggccgcgg cggtcggcct
     1501 ggccggggcg gccgtcggcc tgtcccgccg gtgacgtgcc cgcccggccc cgctggcccg
     1561 ggtcggcacg tcgagccggg aggggtcagc cgcgccgcct cgagtcggcc agggcgtagg
     1621 cgtggtcgag gacctgcacc gggtggtaca cgggcaggcc ggtcgccctc gcgatgtgcc
     1681 agcggcacgt ctcggagtcg cacacgacga actccggccg gacgtcggcg acctggtcga
     1741 acagggtctg gccgacgtcc atggcgatct cgtacttctc gaccttggtg ccgtacgtgc
     1801 ccgcggtgcc gcagcaaccg gccctggacc gctccacctc gagcccgggg acgagcgcca
     1861 ggaggtcgag cgccggcgtg ccgatcccgt gggccatgag ctggcagggc gggtggtaga
     1921 cggcgcgcgc ccggatctcg ccgagcccgg tgtccagctc gccgtcgtcg tggagctcga
     1981 ggagatactc gcagatgtcg tacacggccc gcgagaccgc ggtggtccgc tcgtcctcca
     2041 tgccgaggag ctccctgtac ttggccttga gggtcatgcc gcacgaggtc gagttggcga
     2101 tgatcacgcc cgggtcgctg gccgagggcg agagccgcct gacgagcttg ccggcgtacc
     2161 cccgcgccga gtcgaagtcg ccgttggaga cgagcggcag gccgcagcag ttctgccgcg
     2221 ggtaggtgac cgcgacgccg ttgcgctcga ggacccggac caccgtcgcg ccgacctccg
     2281 gctcgaactc ctc
//
LOCUS       DASPYY010000058         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_149376, whole genome shotgun sequence.
ACCESSION   DASPYY010000058 DASPYY010000000
VERSION     DASPYY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149376"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1411)
                     /locus_tag="VEP73_00770"
     CDS             complement(47..1411)
                     /locus_tag="VEP73_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25004.1"
                     /translation="MRPDGPAPHGGAWPRWTARLRLTLWYGGLFLLAGSVLVATSYLL
                     VHQQLTPSPAAGIAKASTTPANSAEAVVCVSSPGSAEPPQCQTLPPGVDLPTPDPTGG
                     YRVTGGQIITLEHTFLSQAMRTFLQTMLGVLGLMALLSLGLGWVVAGRVLRPLQRITA
                     TAKRLSERTLHQRIALDGPDDELKELADTFDRMLARLDAAFDSQRRFAANASHELRTP
                     LAISRTEVDVALADPDATTAELRAMAERVRDATDRSERLIEGLLTLARSERELRSREP
                     ADLADAAAEALEHARREGGRDANGDHGITGLRVVAGLRPAPVAGDPALLERMVANLVE
                     NAVRHNQPSGWLDVATGTSDGRVYVRVANGGRAIPADQVESLFEPFRRLHRRVASAAC
                     GAGLGLSIVRSVARAHGGDAHARALARGGLEVTVRLPVRAAACAGELVPGRGQRVGHA
                     TAPR"
     gene            complement(1408..2061)
                     /locus_tag="VEP73_00775"
     CDS             complement(1408..2061)
                     /locus_tag="VEP73_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25005.1"
                     /translation="MRVLLVEDEVVLAEAVARGLRHQGMAVDLAFDGTDALEKARVNH
                     YDVLVLDRDLPEVHGDEVCRKLASQGASTRILMLTAAGTVDDRVEGLDLGADDYLGKP
                     FAFSELVARVRALGRRSGGPTPPVLARYDVVLDPARREATRGGHFLRLTRKEFGVLEV
                     LLAADGTAVSAEELLERVWDEHADPFTHTVRVTVGNLRRKLGTPPLIETVIGAGYRL"
     gene            2353..>2965
                     /locus_tag="VEP73_00780"
     CDS             2353..>2965
                     /locus_tag="VEP73_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25006.1"
                     /translation="MHDHSGRATPARPPRRRWRWPHLAALVGALTLALGVAACAGGGK
                     TGGVASLSGSGRPTSTTNANSSEDFRQAALAFARCMRQHGIDMPDPQFSGNRVTQELK
                     AGPGGKGPDDPKFKAAQQACRQYMPNGGQPLKPNAQEQQQMVQFARCMRQHGIAVPDP
                     GPNGGIEVDRGNGRKGPGPDDPKFKAAEQACRQYQPEGGKLQTS"
BASE COUNT          404 a         1093 c         1138 g          330 t
ORIGIN      
        1 cggccccgac aacatcaccg tcgtcctgct gcgcggctgc tgaccatcac ctcggtgccg
       61 tggcgtggcc cacgcgctgc cccctgccgg ggacgagctc tccggcgcag gccgcggcgc
      121 ggaccgggag gcgcacggtg acctccaggc cgccgcgggc gagcgcccgg gcatgggcgt
      181 cgccgccgtg ggcgcgggcg accgagcgga cgatcgacag gcccaggccg gcgccgcagg
      241 cggccgacgc gacccggcgg tgcaggcggc ggaacggctc gaacagcgac tcgacctggt
      301 cggccgggat ggcacggccg ccgttggcga cccgcacgta gacgcgaccg tcgctcgtgc
      361 cggtggcgac gtcgagccag ccggagggct ggttgtggcg cacggcgttt tcgaccaggt
      421 tggcgaccat gcgctccagc agcgcggggt cgccggcgac cggggcgggc cgcagccccg
      481 cgaccacgcg caggccggtg atgccatggt cgccgttggc gtcccggccg ccctcgcgcc
      541 gggcgtgctc gagcgcctcg gcggcggcgt cggccaggtc ggccggctcc cgcgagcgca
      601 gctcccgctc gctgcgggcg agcgtgagca gcccctcgat cagccgctcg ctgcggtcgg
      661 tggcgtcccg gacccgctcg gccatggcgc gcagctcggc ggtggtggcg tccgggtcgg
      721 ccagggcgac gtccacctcg gtgcggctga tcgccagcgg ggtgcgcagc tcgtgcgagg
      781 cgttggcggc gaagcggcgc tgggagtcga aggcggcgtc gagccgggcg agcatccggt
      841 cgaaggtgtc ggccagctct ttgagctcgt cgtcggggcc gtccagggcg atccgctggt
      901 gcagggtgcg ctcggacagg cgcttggcgg tcgcggtgat ccgctgcagc gggcgcagca
      961 cccgcccggc caccacccag ccaagcccca gcgacaacag cgccatcagc cccagcaccc
     1021 ccagcatcgt ctgcaggaac gtgcgcatcg cctgggagag gaaggtgtgc tcgagcgtga
     1081 tgatctgccc gccggtgacg cggtagccgc cggtggggtc cggggtgggc aggtccaccc
     1141 cgggcggcag ggtctggcac tggggcggct cggcggatcc gggggaagac acgcacacca
     1201 cagcctcggc ggagttggcg ggggtggtcg acgccttggc gatcccggcg gcgggcgagg
     1261 gcgtgagctg ctggtggacc agcaggtagc tggtggccac cagcaccgag ccggcgagca
     1321 ggaacagccc gccgtaccac agcgtcaggc gcagccgcgc cgtccagcgc ggccaggcgc
     1381 cgccgtgcgg ggcggggccg tcgggtctca cagccggtac cccgcgccga tcaccgtttc
     1441 gatcagcggc ggcgtgccga gcttgcggcg caggttgccg accgtgaccc ggacggtgtg
     1501 ggtgaacggg tcggcgtgct cgtcccacac ccgctcgagc agctcctcgg cgctgaccgc
     1561 cgtgccgtcg gcggccagca gcacctccag cacgccgaac tccttgcggg tcagccgcag
     1621 gaagtggccg ccgcgggtcg cctcgcgcct ggccgggtcg aggacgacgt cgtagcgggc
     1681 cagcaccggc ggggtcggcc cgccgctgcg ccgccccagc gcccgcacgc gcgcgaccag
     1741 ctcgctgaag gcgaacggct tgccgaggta gtcgtcggcg ccgaggtcca ggccctcgac
     1801 gcggtcgtcg accgtgcccg cggccgtcag catcaggatc cgcgtgctgg cgccctggct
     1861 ggcgagcttg cggcagacct cgtcgccgtg cacctcgggc aggtcgcggt ccagcaccag
     1921 cacgtcgtag tggttgaccc gggccttctc gagcgcgtcg gtgccgtcga aggcgaggtc
     1981 gacggccatg ccctggtggc gcaggccgcg ggcgaccgcc tcagccagca ccacctcgtc
     2041 ctccaccagc agcacgcgca cggcaggccc tcctcgacgc ccggacctca cgagttggac
     2101 cggccgcgcg ggcaggacgt gcccgcccgc tgtcgcggct cgtcgcacga gccgaccggc
     2161 cggcgtgacc agggtgcgcc cgcccgcgtc acgtccgcgt aaagcaaacg cgagacgcgg
     2221 acattacccc ctgccaggta gaccggtacg cgaccggggc gagcggcgcc ccggcccgcc
     2281 cggggccgca cgccccggag aacccgtccg ggggcacgcg cccgagaggt ccgtaaccag
     2341 gaggagcgtc tcatgcacga tcacagcggc cgggcaaccc cggcacgccc accgcgtcgg
     2401 cgctggcgct ggccgcacct ggccgcgctc gtcggcgcgc tgacccttgc gctcggtgtc
     2461 gcggcctgcg cgggcggcgg caagaccggc ggggtggcca gcctcagcgg gtccgggcgg
     2521 cccaccagca ccaccaacgc gaacagcagc gaggacttca ggcaggcggc gcttgcgttt
     2581 gccaggtgca tgcgccagca cggcatcgac atgcccgacc cgcagttcag cgggaaccgg
     2641 gtcacccagg agctcaaggc gggtcccggc ggcaaaggcc cggacgaccc gaagttcaag
     2701 gcggcgcagc aggcctgccg gcagtacatg ccgaacggcg gccagcccct caagcccaac
     2761 gcgcaggagc agcagcagat ggtgcagttt gcccggtgca tgcgccagca cggcatcgcc
     2821 gtgcccgacc cgggcccaaa cggcgggatc gaggtagaca gggggaacgg ccggaaaggg
     2881 cccggcccgg acgacccgaa gttcaaggcg gcggagcagg cctgccggca gtaccagccc
     2941 gagggcggca agctgcagac cagcg
//
LOCUS       DASPYY010000059         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_711191, whole genome shotgun sequence.
ACCESSION   DASPYY010000059 DASPYY010000000
VERSION     DASPYY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711191"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VEP73_00785"
     CDS             complement(<1..381)
                     /locus_tag="VEP73_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYT25007.1"
                     /translation="MNRRGDRPGPPAGGGALPRVAVVGRPNVGKSTLVNRFLGRRAAI
                     TEEAPGVTRDRVPYEADWSGRRFELVDTGGWEPRATGLAARVVAQAEQAMAESDLVLF
                     VVDASVGVTDEDAAVAARLRRGGVP"
     gene            complement(386..1081)
                     /locus_tag="VEP73_00790"
     CDS             complement(386..1081)
                     /locus_tag="VEP73_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT25008.1"
                     /translation="MTTAGADRGYRTAATVVKPLMRAWFRIRLDGAEHIPPAGPAILA
                     ANHRSNVDPVLLACAVERPVFFMSKAELFVWPLGPILRWIGQFPVRRGGADREALRQT
                     AAVIARGGMLGLFPEGSRGEGRFATVHEGLAYIVLRERCPVLPVAMLGTERVRRRAGW
                     LPFASPVRVVVGPPVALPDPGVGRTGRRAATEALRRTLHDFLDGVESGATATTRTGSP
                     PADERHIDKPMDE"
     gene            complement(1078..1773)
                     /gene="cmk"
                     /locus_tag="VEP73_00795"
     CDS             complement(1078..1773)
                     /gene="cmk"
                     /locus_tag="VEP73_00795"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYT25009.1"
                     /translation="MIMEELDEGQHAPDDVVVAIDGPAGSGKSTVARAVAERADLRHL
                     DTGATYRALTLALLRQGVAPDDADAVAVAAKGADLALELAPGRSGTVRVLLDGSAMGS
                     ELRSPEVSAAVSAVSAVPAVREQLVALQRAVISLGGVVAEGRDVGTVVWPQAEVKVFL
                     TADAAERARRRAEGESAEALARRDRLDSGRASAPTRPAADALLVDSTGREVDDVVGQI
                     LGLVSSARAGRPR"
BASE COUNT          216 a          721 c          709 g          201 t
ORIGIN      
        1 cggcacgccc ccccggcgca ggcgcgccgc gacggcggcg tcctcgtcgg tgacgccgac
       61 gctcgcgtcc acgacgaaca gcacgaggtc ggactctgcc atggcctgct cggcctgggc
      121 cactaccctg gcggccaggc cggtggcccg cggctcccag cccccggtgt cgaccagctc
      181 gaagcggcgg ccggaccagt ccgcctcgta cggcacccgg tccctggtca cgccgggcgc
      241 ctcctcggtg atggcggcgc gccggccgag gaagcggttg accagggtgg acttgcccac
      301 gttgggccgt ccgaccacgg cgacgcgcgg cagcgccccg ccgccggccg gcgggcccgg
      361 gcgatcgcct cgtcgattca tgagctcatt catccatggg cttgtcgatg tgtcgctcgt
      421 cggccggcgg ggagccggtc cgcgtcgtcg ccgtggcgcc cgactcgacg ccgtccagga
      481 agtcgtggag ggtccggcgc agcgcctcgg tggccgcacg ccggccggtc cggccgacgc
      541 cggggtccgg cagcgcgacg ggcggcccga cgacgacgcg gaccggcgag gcgaagggca
      601 gccagccggc acgccgccgc acccgctccg tgccgagcat ggccacgggc agcacagggc
      661 agcgctcgcg cagcacgatg taggcgaggc cctcgtggac ggtggcgaac cgcccctcgc
      721 cgcggctgcc ctctgggaac agcccgagca tgccgccccg cgcgatcacc gcggcggtct
      781 ggcgcagcgc ctcccggtcg gcgccccccc gccgcaccgg gaactggccg atccagcgca
      841 ggatcggccc gagcggccag acgaacagct cggccttgct catgaagaac accggccgct
      901 cgaccgcgca cgccagcagg accgggtcga cgttcgagcg gtggttggcg gcgaggatcg
      961 cggggccggc gggcgggatg tgctcggcgc cgtcgagccg gatgcggaac cacgcccgca
     1021 tcagcggctt cacgacggtc gcggccgtgc ggtagccacg atccgcgccc gccgtggtca
     1081 ccgcggccgt ccggccctgg ccgaggagac gagcccgagg atctgcccga cgacgtcgtc
     1141 cacctcgcgg ccggtcgagt cgaccagcag cgcgtcggcc gccggcctgg tgggggccga
     1201 cgcccggccg ctgtccagcc ggtcgcgccg ggcgagcgcc tcggccgact cgccctcggc
     1261 cctgcggcgg gcacgctcgg ccgcatcggc ggtgaggaag accttcacct cggcctgcgg
     1321 ccacaccacc gtgccgacgt cccgcccctc ggcgaccacg ccgcccaggc tgatgacggc
     1381 gcgctgcagg gcgacgagct gctcgcgcac cgccgggacg gccgagacgg ccgagacggc
     1441 ggcgctcacc tcgggcgagc ggagctcgct gcccatcgcg gagccgtcca gcagcacccg
     1501 caccgtgccc gagcggcccg gggcgagctc gagggcgagg tcggcgccct tcgcggcgac
     1561 ggccaccgcg tcggcgtcgt ccggcgcgac gccctggcgc agcagggcca gggtgagcgc
     1621 ccggtaggtg gcgccggtgt cgaggtgccg gaggtcggca cgctccgcca ccgcccgggc
     1681 gacggtgctc ttgcccgagc cggccgggcc gtcgatcgcg acgacgacgt cgtcgggggc
     1741 gtgctggccc tcgtccagct cctccatgat catgcggcct tcccgtcggt ccggcacgcg
     1801 gcgagggcgg ggcgggcgct ggcgccacgc ggtcgaggcg gggggtt
//
LOCUS       DASPYY010000060         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1695548, whole genome shotgun sequence.
ACCESSION   DASPYY010000060 DASPYY010000000
VERSION     DASPYY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695548"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VEP73_00800"
     CDS             complement(<1..352)
                     /locus_tag="VEP73_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT25010.1"
                     /translation="MAKQTADVDSLVGRTLGRRRYEVVALVAQGGMATVYRAHDRQLD
                     RVVAVKVPRPEFARDAGFSEQFRREARAAARLSHPNVVAVYDSGEDRGLPWIVMEYVS
                     GQTLRDLLERRGRLD"
     gene            510..842
                     /locus_tag="VEP73_00805"
     CDS             510..842
                     /locus_tag="VEP73_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25011.1"
                     /translation="MAFDDDLRMVGFRLTQDRRDGTRTWSLPRNGYLTYWLHVPPTDG
                     IALFTWEFAIGEYMNEYGLQIGTNEPLNQFLFPQDDTEVPEDLGAVVKAMERTEAMFA
                     GMDFGHRG"
     gene            940..1119
                     /locus_tag="VEP73_00810"
     CDS             940..1119
                     /locus_tag="VEP73_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25012.1"
                     /translation="MRRGGSLLVTILLLFGAFYLGVLYERHDCKIDLPQTAGQVGKSV
                     ACRDYRSNINTNVPK"
     gene            1202..1573
                     /locus_tag="VEP73_00815"
     CDS             1202..1573
                     /locus_tag="VEP73_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HYT25013.1"
                     /translation="MLGRGTYLALLAACVLVTLPLELVLHARVYARPRRWLASLLPVF
                     VVFSLWDYVAIRRGHWSYDPRYTTGVAFFGHVLPLEELVFFVVVPTCAILAYQAVSRL
                     LGPVPPRDRPEEAGRPGRRRR"
     gene            1570..>1781
                     /locus_tag="VEP73_00820"
     CDS             1570..>1781
                     /locus_tag="VEP73_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HYT25014.1"
                     /translation="MREYTLAAAAGVVLAVALETAVLRTGMFRDRRYWAALAIVAGFQ
                     LVVNGVLTCTPIVTYDPRRILGLRVA"
BASE COUNT          237 a          640 c          636 g          268 t
ORIGIN      
        1 ggtcgaggcg gccccgccgc tcgagcaggt cgcgcagggt ctggccgctg acgtactcca
       61 tcacgatcca gggcagcccc cggtcctcgc cggagtcgta gaccgcgacg acgttcgggt
      121 gcgacaggcg cgcggcggcg cgcgcctcgc ggcggaactg ctcggagaag ccggcgtcgc
      181 gagcgaactc ggggcggggc accttgacgg ccacgacccg gtcgagctgg cggtcgtggg
      241 cgcggtagac ggtcgccatg ccgccctgag cgaccagggc gaccacctcg tagcggcgcc
      301 tgccaagggt acggccgacg aggctgtcga cgtcggcggt ctgctttgcc acgcgcggtc
      361 ccagcggatg gtcgggtcgt ggcccggcgg gtcccgggtg ccagccgaga ttgtaagcca
      421 acggggccac atcggccccg ctggcggatc cagccgtccg accggacaat gccctgcgac
      481 cctccgacgc gaaggagccc ggcccgacga tggcgttcga cgacgatctc cggatggtgg
      541 gattccgcct tacccaggac aggcgcgacg ggacccggac gtggtccctg ccccgcaacg
      601 gctacctcac ctactggctg cacgtgccgc cgaccgacgg catcgccctg ttcacctggg
      661 agttcgccat cggcgagtac atgaacgagt atggcctgca gatcggcacg aacgagccgc
      721 tgaaccagtt cctgttcccc caggacgaca ccgaggtgcc agaggacctg ggcgcggtgg
      781 tcaaggcgat ggagcggacg gaggcgatgt tcgccggcat ggacttcggg caccggggct
      841 gagcgccggg ggcgattgca agcggttcga gcagggtagg ccgagttcgt tccagaatgg
      901 cgctctcatc cggcaggcat ccctcgagga ggctcccaaa tgcgtcgcgg tggctcgctg
      961 ctcgtcacca tcctgctcct gttcggggcg ttctacctcg gcgttctcta cgagcgccac
     1021 gactgcaaga tcgacctgcc ccagaccgcc gggcaggtcg gcaagtcggt cgcgtgccgc
     1081 gactaccgct ccaacatcaa cacgaacgtc ccgaagtaga cgcggctctc cgcctgcgga
     1141 tccccgccgg acgcggtacg gtcggccccg gcgggccccc atgcgcttgg gaggcgaggc
     1201 ggtgctcgga cgggggacct acctcgcgct gctggcggcc tgtgtgctcg tcaccctgcc
     1261 gctcgagctc gtcctgcacg cccgcgtgta cgcacggcca cggcgctggc tcgcgagcct
     1321 gctgccggtg ttcgtggtgt tcagcctctg ggactacgtg gcgatccgcc gcggccactg
     1381 gtcctacgac ccccgctaca cgaccggggt cgccttcttc ggccacgtcc tgccgctcga
     1441 ggagctggtc ttcttcgtcg tggtgccgac ctgcgcgatc ctcgcctacc aggcggtgag
     1501 ccgcctcctc gggccggtcc cgccgcggga ccggcccgag gaggccggcc ggcccggccg
     1561 gaggcgccgg tgagggagta cacgctggcc gcggcggccg gggtcgtgct cgcggtggcg
     1621 ctcgagacgg cggtgctgcg gaccggcatg ttccgcgacc ggcgctactg ggccgcgctc
     1681 gccatcgtgg ccgggttcca gctcgtcgtg aacggggtcc tcacctgcac cccgatcgtg
     1741 acctacgacc cgcgccgcat cctcggcctc cgggtcgcct g
//
LOCUS       DASPYY010000061         1904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_150418, whole genome shotgun sequence.
ACCESSION   DASPYY010000061 DASPYY010000000
VERSION     DASPYY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150418"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="VEP73_00825"
     CDS             complement(<1..1902)
                     /locus_tag="VEP73_00825"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HYT25015.1"
                     /translation="MDGAEQVRVAVLGMGKLGGEELNYVSDIDVLFCHQAAPDPSRGP
                     AAATGSARGSSGRGTMPPEPPGAEIGADEAGRIAAALARELIGGLSAVTAEGTCYRVD
                     ANLRPEGRNGPLTRTLGSYQAYWERWAEAWEQQALLKVRPVAGDRALGEDFHQAAAAW
                     TYPERLDPEAIAAIRHMKVRVEASQQARAGGERQVKLGPGGLRDVEFAVQLLQLVHGR
                     HDAALRSGSTLVALEALSAAGFVGRSDAAALASAYRFLRMIEHRLQLASELRTHTIPP
                     GEAERRWLARTMGYRDGPDGDALELFEADRRRHAATVRGLHEKLFYRPLLEVFGAVPT
                     GLDPQEARERLAALGYANPDRAMAHLRALTSGLSRRATLFRAMLPAMLTWLAEAPDPD
                     GGLGALRVVAEALGRHGAFLATLRDNPAVAELLCTVLGSSRLLGDLLARHPELLTAMA
                     EAGGPAGARTRDQLVGEALAIVARHLGHGHREGDAGLGGGSAAEVADAWDGLRRFKRR
                     ELLRIAVRDLVNQVEVAVVGQELAALAEACLEAGLAVATRRVDGGEQVRVAVLGMGKL
                     GGEELSYTSDLDVLLCHEPAEGADPSRAAHAAAQVARELLSGLGAVTREGTGFKVDAN
                     LRPEGRNGPL"
BASE COUNT          239 a          700 c          732 g          233 t
ORIGIN      
        1 cagcgggccg ttgcgcccct cgggccgcag gttggcgtcc accttgaaac cggtcccctc
       61 gcgggtgacc gccccgagcc cgctgagcag ctcgcgggcg acctgggcgg cggcgtgggc
      121 tgcccggctt gggtcggcgc cctcggcagg ctcgtggcac agcaggacgt ccaggtcgga
      181 ggtgtagctc agctcctcgc cgccgagctt gcccatgccg agcaccgcca cccgcacctg
      241 ctcgccgccg tccacgcggc gggtggcgac tgccaggcca gcctccaggc acgcctcggc
      301 gagtgccgcc agctcttggc cgaccaccgc cacctcgacc tggttgacca gatcccggac
      361 ggcgatccgc agcagctccc gccgcttgaa gcggcgcagc ccgtcccagg cgtcggccac
      421 ctccgcagcg ctgcctccac ccaaaccggc gtcgccctca cggtggccgt ggccgaggtg
      481 gcgggcgacg atcgccaggg cctcccccac cagctggtcg cgggtgcggg cgccggccgg
      541 gccaccggcc tcggccatgg cggtgagtag ctcggggtgg cgggcgagca ggtcgccgag
      601 cagccgggag ctgcccagca cggtgcagag cagctcggcc acggcggggt tgtcccgcag
      661 ggtggcaagg aaggcgccgt gccggccgag cgcctcggcg acgacccgca gggcgccgag
      721 gccgccgtcc gggtcgggtg cctcggccag ccaggtgagc atggcgggca gcatggcgcg
      781 gaacagggtg gcacggcggg acaggccgct ggtgagcgcc cgcaggtgcg ccatggcccg
      841 gtcggggttg gcgtagccga gcgcggcgag ccgctcgcgg gcctcctgcg gatccaggcc
      901 ggtggggacc gccccgaaga cctccagcag cggccggtag aagagtttct cgtggaggcc
      961 gcggacggtg gccgcgtggc ggcggcggtc ggcctcaaaa agctccagcg cgtcgccgtc
     1021 ggggccgtcg cggtagccca tggtgcgggc gagccagcgg cgctcggcct cgcccggcgg
     1081 aatggtgtgg gtgcgaagtt cgctggcgag ctgcaggcgg tgctcgatca tgcgcaggaa
     1141 gcggtaggcg ctggcgagcg cagccgcgtc gctgcggccg acgaacccgg cggcgctgag
     1201 cgcctccagc gccaccagcg tggagccgct gcgcagcgcc gcatcatggc ggccgtggac
     1261 caactgcagg agctgcacag cgaactcgac gtcgcgcagc cccccagggc cgagcttgac
     1321 ctggcgttcg ccgcccgcgc gggcctgctg ggaggcctct acgcggacct tcatgtggcg
     1381 gatcgcggcg attgcctcgg ggtcgagccg ttccgggtag gtccaggcag cggcggcctg
     1441 gtgaaagtcc tcgccgagag cgcggtcccc ggcgaccggg cggaccttga gcagcgcctg
     1501 ctgctcccac gcctccgccc agcgctccca gtaggcctga taggagccaa gcgtgcgggt
     1561 cagcgggccg ttgcgcccct cggggcggag gttggcgtcc acgcggtagc aggtgccctc
     1621 cgccgtgacg gcgctgaggc cgccgatcag ctcccgcgcg agcgcggcgg cgatccgccc
     1681 agcctcgtcg gcgccaatct ccgcgccggg gggttcgggg ggcatggtcc cgcggccgga
     1741 agaaccccga gccgatcccg tggcagccgc ggggcccctc gatgggtcag gggcggcctg
     1801 gtggcagaag agcacgtcga tatcggagac gtagttcagc tcctcgccgc cgagcttgcc
     1861 catgccgagc acagccaccc gcacctgctc ggcgccgtcc accc
//
LOCUS       DASPYY010000062         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1976382, whole genome shotgun sequence.
ACCESSION   DASPYY010000062 DASPYY010000000
VERSION     DASPYY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976382"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VEP73_00830"
     CDS             <1..1237
                     /locus_tag="VEP73_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYT25016.1"
                     /translation="VAELEPGDEAGVTAWAGDLSTPPPPAREVVDAARSRAEGIAARS
                     EPADDVDETLAVAADQFVVAGPTVVAGYPWFGDWSRDTMTSYEGLFLTTNRWDEGRAL
                     LMRAAGTLSAGMLANTADVGGMPEFNTADGTLWFLHAVGRHVQITGDLDLGAALCPDL
                     EAVVANHVVDTRFGIGVDRSDGLLTQGQDGWALTWMDARVDGRPVTARAGKAVEVNAL
                     WISGLAAVAGVQEAVGRDSSATRTLEAKARRSFVRRFARPDGGLLDVVDGPSGDSPEL
                     RPNQLLAVSLPDAPVRDPGVVSACGPLLTSLGLRSLGPAEPGYLGRHRGGPAERDRAY
                     PQGTVWPWLIGPYAEAARKTGVSTEGLLDGLEAHLQEWGLGSVSETADGDPPHAATGC
                     PFQAWSVAEVLRARRLLRA"
     gene            complement(1244..>1534)
                     /locus_tag="VEP73_00835"
     CDS             complement(1244..>1534)
                     /locus_tag="VEP73_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25017.1"
                     /translation="AVFSPRLAGFVLDAFGSGADAVGGLAASADPELDQLTPREREVL
                     RHIARGYAYKQIARQLHISVKTVETHVSAVLRKLQLSSRYELTRWATDRRLV"
BASE COUNT          191 a          523 c          613 g          207 t
ORIGIN      
        1 cgtcgccgag ctggagccgg gcgacgaggc cggggtgacc gcgtgggcgg gggacctatc
       61 gaccccaccg ccaccggcgc gcgaggtggt cgacgcggcc cggagccggg ccgaggggat
      121 cgcagcccgg tcggagcccg ccgacgacgt cgacgagacg ctcgccgtcg cggccgacca
      181 gttcgtggtg gccggcccca cggtggtggc gggctacccg tggttcggcg actggtcgag
      241 ggacaccatg acctcctacg aggggctgtt cctgacgaca aatcggtggg acgagggccg
      301 ggccctcctc atgcgggcgg ccggcacgct ctccgcgggg atgctcgcca acaccgccga
      361 cgtcgggggg atgcccgagt tcaacaccgc cgacgggacg ctgtggttcc tgcacgccgt
      421 ggggcggcac gtgcagatca ctggggacct cgacctgggc gccgcgctgt gtcccgacct
      481 ggaggccgtg gtggcgaacc acgtggtgga cacgcggttc gggatcgggg tggaccgctc
      541 cgacggcctc ctgacccagg gacaggatgg gtgggccctc acgtggatgg acgccagggt
      601 ggacggccgg ccggtgaccg cgcgggcggg caaggcggtc gaggtgaacg cgctgtggat
      661 ttccggcctg gcggcggtgg cgggggtgca ggaggcggtg gggcgcgaca gctcggcgac
      721 gcgcacgctc gaggcgaagg cccgccgatc gttcgtccgg cggttcgccc ggcccgacgg
      781 cggcctgctg gacgtggtgg acggtccgtc cggcgattcc ccggagctca ggccgaacca
      841 gctgctcgcg gtgtccctgc cggacgcgcc ggtgcgcgac ccgggcgtgg tttcggcctg
      901 cgggccgctc ctgacctccc tggggctgcg gtctctggga ccggcggagc ccggatacct
      961 gggccggcac cgaggaggtc cggccgagcg tgaccgggcc tatccccagg gcacggtgtg
     1021 gccctggctg atcggtccgt acgccgaagc cgcccgcaag accggtgtgt ccaccgaggg
     1081 cctcctggat gggctggagg cgcacctcca ggaatggggg ctcggatccg tctccgagac
     1141 cgccgacggc gacccgcccc acgccgccac ggggtgtccc ttccaggcct ggtcggtggc
     1201 ggaggtgctt cgagcccgcc gcctgctccg ggcttaggtt ctttcagacc aggcggcggt
     1261 cggtggccca gcgggtcagc tcgtaccggc tcgagagctg gagtttccgc aggaccgccg
     1321 agacgtgggt ctccacggtc ttcaccgaga tgtggagctg ccgggcgatc tgcttgtacg
     1381 cgtatccgcg ggcgatgtgg cgcaggacct cccgctcgcg cggggtgagc tggtcgagct
     1441 cgggatcggc cgacgcggcc aggccgccga ccgcgtccgc gcccgagccg aacgcgtcga
     1501 gcacgaagcc ggccagccgg ggcgagaaga ccgc
//
LOCUS       DASPYY010000063         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2257648, whole genome shotgun sequence.
ACCESSION   DASPYY010000063 DASPYY010000000
VERSION     DASPYY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257648"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VEP73_00840"
     CDS             <1..941
                     /locus_tag="VEP73_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HYT25018.1"
                     /translation="GLMCRLAAYLGPPAPLSVLLYDPPWSLAEQAYRPRRQEYGTVNV
                     DGTAVVWWPPGEAEPLRYATERPPWADPNLPLLAPRLHATVQLAAVRSATPGLPFGAD
                     AAPPFLHGGLALAHNGRIEGFRGALGRRLLASLPDDLYGALGPLPLHDSVLLLLLTAW
                     GRRERPEAGGSGGTVPRLEGPQADPMVAAGPPDGSRSLAGALGAAVTRVAGACAEAGE
                     RATLTALAAEHGHLAGVRVAVGLASPALFLHLPTGPSGSESEAALVASEPLDDRSGWT
                     EVPDRHLLDLSAAGATLTPLDLNGLDAGTHTMERSA"
     gene            938..>1805
                     /gene="egtD"
                     /locus_tag="VEP73_00845"
     CDS             938..>1805
                     /gene="egtD"
                     /locus_tag="VEP73_00845"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HYT25019.1"
                     /translation="MTTRTLPSVTSLAPPADWRGQLAADVRAGLTANPRTLPSKYFYD
                     AEGSELFEQITRVPEYYLTRAEHEILSDHADQLLAAAQASEVLELGSGSSRKTRLLLE
                     AMHRLGDRLGDRDSGGQRYAPLDVSEDALIGAALALCNDYPWLRFDGYVGDFTADLPK
                     VPRHGRRLVVLLGSTIGNLAPEERAGLLAGVSAACQPGDRFLLGVDLVKDSATLEAAY
                     DDAAGVTAAFNRNVLQVLARELDAAVPVEAFEHAARYDERRARIEMHLRASQDLVLEF
                     PSLDLTVPLAAGE"
BASE COUNT          216 a          719 c          636 g          234 t
ORIGIN      
        1 ccgggctgat gtgccggctt gccgcctatc tcggcccgcc cgcgccgctg tcggtgctgc
       61 tctacgaccc gccgtggtcg ctggcggagc aggcctaccg gccccgccgc caggagtacg
      121 gcaccgtcaa cgtggacggc accgcggtgg tgtggtggcc gcccggcgag gcggagccgc
      181 tccgctacgc caccgagcgg ccgccctggg ccgatccgaa cctgccgctg ctcgccccga
      241 ggctgcacgc cacggtgcag ctcgcggcag tccgttccgc tacccccggc ctgccgttcg
      301 gcgccgacgc cgccccgccg ttcctgcatg gaggcctggc tctggcgcac aacggccgca
      361 tcgaggggtt ccgcggggcg ctcgggcggc ggctgctcgc cagcctcccc gacgacctgt
      421 acggggcgct cggcccgctg ccgctgcacg actcggtgct gttgctgctg ctgaccgcgt
      481 gggggcgccg cgagcggccg gaggcggggg gttcgggggg cacggtcccg cggctggaag
      541 gaccacaagc cgatcccatg gtggccgcgg ggccccccga tgggtcaagg agcctggccg
      601 gcgcgctcgg cgccgccgtg acccgggtcg ccggcgcctg cgccgaggcc ggtgagcggg
      661 cgaccctcac cgcgctggcc gccgagcacg gccacctggc cggggtgcgc gtggcggtgg
      721 gcctcgcctc gcccgcgctg ttcctgcacc ttcccaccgg gccgtcgggg tctgagagcg
      781 aggcggccct ggtcgcctcc gaaccgctcg acgaccgctc cggctggacc gaggtccccg
      841 accggcacct gctcgacctc agcgccgccg gggctacgct gacgcccctg gacctgaacg
      901 gcctggacgc cggcacgcac accatggaga gatccgcatg acgacccgca ccctcccttc
      961 ggtcaccagc ctcgcgccgc ccgcggactg gcgggggcag ctcgccgccg acgtccgcgc
     1021 cggcctcacc gccaaccccc ggacgcttcc ctccaagtac ttctacgacg ccgagggctc
     1081 ggagctgttc gagcagatca cccgcgtgcc cgagtactac ctcacccgcg ccgagcacga
     1141 gatcctcagc gaccacgccg accagctgct ggcggcggca caggccagcg aggtgctgga
     1201 actgggctcc ggctcgtcac gcaagacccg gttgctgctg gaggcgatgc accgcttggg
     1261 cgaccgcttg ggcgaccgcg acagcggcgg gcagcgctac gcccccttgg acgtcagcga
     1321 ggacgccctc atcggcgcgg cgctggccct ctgcaacgac tacccgtggt tgcgcttcga
     1381 cggctatgtc ggcgacttca ccgccgacct gcccaaggtg ccccgccacg gccgccgcct
     1441 ggtggtgctg ctcggctcca ccatcggcaa cctcgcgccg gaggaacgcg ccggcctgct
     1501 ggcgggggtg tccgccgcat gccagcccgg cgaccgcttc ctgctcggcg tcgacctggt
     1561 caaagactcc gccacgctgg aggcggccta cgacgacgcg gccggggtga ccgccgcctt
     1621 caaccgcaac gtcctgcagg tgctcgcccg ggagctggac gccgccgtcc ccgtcgaggc
     1681 gttcgagcac gccgcccgct acgacgagcg ccgagcccgc atcgagatgc acctgcgcgc
     1741 gagccaggac ctggtgctgg agttcccgag cctcgacctg accgtcccgc tcgcggcggg
     1801 cgaga
//
LOCUS       DASPYY010000064         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1415020, whole genome shotgun sequence.
ACCESSION   DASPYY010000064 DASPYY010000000
VERSION     DASPYY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415020"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VEP73_00850"
     CDS             complement(<1..518)
                     /locus_tag="VEP73_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYT25020.1"
                     /translation="MTEPATVAPNRPLDVAVIIPAFNAGHYLDQALASVAGQTLAPAV
                     VVVADDGSTDDTVARARRWRDHLPVEVVRLGRNAGPGPARHRAILATDAPVLAMLDAD
                     DLWLPDHLETMTAAYERAPGLVTAREFAWIRGRGIDLARRRARAPIPTDPDEQLAALL
                     QHNYVSFGFFPRT"
     gene            635..1786
                     /locus_tag="VEP73_00855"
     CDS             635..1786
                     /locus_tag="VEP73_00855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25021.1"
                     /translation="MGDRGGLRLLVRPRLGPLEPAWDALVERLPLPSPFLRSWWLEQT
                     ARGVPRFLLVVDAGELVGGLALQEERWLGVPRLRVMGAGALCPDHLDLVALPGREEDV
                     AAALAAWLRRPGSRVLDLEGVAGGSRLAAALPSPVRREVVAVAPWAPLTEDPDEWLRT
                     RPRGFRATLRKAARRLDEQAAAHRVLRGAQVDAALAALRRLHGAQWGERSNFLAAYDR
                     FAAASRAGASRGEVVVHELAAGDEVVAVVSCFEVAGRVSLYQSGRLCAHGWRNAATVL
                     LVEVIRDACRRGLTEVDLLRGDEPYKAGFASAARELLRLRAAHGAAGRLALAALVLGT
                     AVRKLAGRRLRLRRRRALPAPSAAGQAAGQAAPQGVRAHGGDLGLVRQR"
     gene            complement(1670..>2205)
                     /locus_tag="VEP73_00860"
     CDS             complement(1670..>2205)
                     /locus_tag="VEP73_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25022.1"
                     /translation="RGMDMRRRVGFVFVSSPNSTTPAHFDIEHSLIMQMHGQKRLTFG
                     RFETPEARRHEVVRYWDGSHGRIEALPPELVRFDLVRGMGVYIPPVTPHWVRNGDAVS
                     VSVTLTFFTPDTDRESLVQAFNARLRRVRLSPRWPGESPLADQAKVTAMRAYALRRRL
                     PRRLPGRRRSGKGAPTT"
BASE COUNT          246 a          827 c          856 g          276 t
ORIGIN      
        1 gtgcgtggga agaacccgaa gctgacgtag ttgtgctgca gcagcgcggc gagctgctca
       61 tcggggtcgg tggggatcgg cgcccgcgcc cgccgccgcg cgaggtcgat cccgcgcccc
      121 cggatccagg cgaactcccg cgcggtcacg agcccagggg cgcgctcgta cgccgccgtc
      181 atcgtctcca ggtggtcggg gagccacagg tcgtcggcgt cgagcatcgc cagcaccggg
      241 gcgtccgtcg ccaggatcgc ccggtggcgc gcgggcccgg gcccggcgtt ccgccccagg
      301 cgcacgacct cgaccggcag gtggtcccgc cagcgccggg cccgcgctac cgtgtcgtcg
      361 gtcgagccgt cgtcggcgac cacgaccacg gcgggggcga gggtctggcc ggcgaccgac
      421 gcgagcgcct ggtccaggta gtgcccggcg ttgaacgcag ggatgatgac cgccacgtcc
      481 agcgggcggt tcggcgccac ggtggcgggc tcggtcatcg acgatgatcg caacagcaga
      541 tgatcgcaac agcggggaag acggtccgcg gctgacccta ccgcagcagc cgcgacctgc
      601 caaccaggat gccggcgctg ggaggggacg ggacatgggc gaccggggcg gcctgcgcct
      661 gctcgtgcgg ccccgcctcg ggccgctgga gcccgcgtgg gacgccctcg tcgagcgcct
      721 ccccctgccc tcgccgttcc tgcggtcctg gtggctcgag cagaccgcgc gcggcgtccc
      781 ccgcttcctg ctggtcgtgg acgccggcga gctggtcggc gggctcgcgc tgcaggagga
      841 gcgctggctc ggcgtgccgc gcctgcgcgt gatgggcgcg ggcgccctct gccccgacca
      901 cctcgacctc gtcgccctcc cgggccggga ggaggacgtc gccgccgcgc tggccgcctg
      961 gctgcgccgg cccgggtcgc gggtgctcga cctcgagggc gtggccggcg gctcccgcct
     1021 cgcggcggcg ctgccctcac cggtgcgccg cgaggtcgtc gccgtggccc cctgggcgcc
     1081 cctcaccgaa gaccccgacg agtggctccg cacccgcccg cggggcttcc gcgccacgct
     1141 ccggaaggcc gccaggcggc tcgacgagca ggcggccgcg caccgggtgc tccggggcgc
     1201 ccaggtcgac gccgcgctcg ccgccctgcg gcgcctgcac ggcgcccagt ggggcgagcg
     1261 gtccaacttc ctggccgcct acgaccgctt cgcggcggcc agccgggccg gcgcgtcgcg
     1321 cggcgaggtc gtcgtccacg agctggccgc gggcgacgag gtcgtcgccg tggtgagctg
     1381 cttcgaggtc gccggcaggg tgagcctgta ccagagcggg cggctgtgcg cgcatggctg
     1441 gcgcaacgcg gcgaccgtcc tgctcgtcga ggtgatccgg gacgcctgcc ggcgcgggct
     1501 caccgaggtc gacctcctgc ggggcgacga gccgtataag gcgggcttcg cctccgccgc
     1561 acgggagctc ctgcggctcc gggccgcgca cggggcggcc ggccgcctgg cgctggccgc
     1621 gctggtcctg ggcacggcgg tgcggaagct cgccggccgc cggctgcggc tacgtcgtcg
     1681 gcgcgccctt cccgctcctt cggcggccgg gcaggcggcg gggcaggcgg cgccgcaggg
     1741 cgtacgcgcg catggcggtg accttggcct ggtccgccag cggtgactcg cccggccacc
     1801 tgggcgacag gcgcacgcgc cgcaggcgcg cgttgaacgc ctgcaccagc gactcccggt
     1861 cggtgtccgg cgtgaagaac gtgagcgtga cggagaccga caccgcgtcc ccgttgcgga
     1921 cccagtgggg cgtgaccggc gggatgtaga cgcccatgcc ccgcacgagg tcgaaccgga
     1981 cgagctccgg cggcagcgcc tcgatgcgtc cgtgggagcc gtcccagtac cgcacgacct
     2041 cgtgccgccg ggcctcggga gtctcgaacc tgccgaacgt cagccgcttc tggccgtgca
     2101 tctgcatgat gaggctgtgc tcgatgtcga agtgcgcggg ggtcgtggag ttcggcgacg
     2161 agacgaacac gaaccccacg cgcctgcgca tgtccatgcc gcgca
//
LOCUS       DASPYY010000065         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1274673, whole genome shotgun sequence.
ACCESSION   DASPYY010000065 DASPYY010000000
VERSION     DASPYY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274673"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEP73_00865"
     CDS             <1..462
                     /locus_tag="VEP73_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT25023.1"
                     /translation="EGRGTFPALTVDENLRLGAHTRRDTAGIRADLGRWYGVFPRLAE
                     RRGQPAGSLSGGEQQMLAIARALMLRPRLLLLDEPSLGLAPLVTRELFEVLARVKGEQ
                     GTTMVLVEQNASLALGLADHAHVLEAGRAVLAGPAGRIRADEHVRRAYLGW"
     gene            472..1353
                     /locus_tag="VEP73_00870"
     CDS             472..1353
                     /locus_tag="VEP73_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT25024.1"
                     /translation="MGRFLQVVVDGIADGSIYATLALALVLIFRSTGIVNFAQGEMAM
                     FSTFVAWGLADAGWPLWLALLGALAVSFLGGMAIERVLIRPVEGGRPLTMVIVTLGLF
                     VLVNSAAGWIWGFSNRGFPRIFPAGTARLGQVAFSLESLGIIAVLLAVVGLLWLLFQH
                     TRAGLAMRAVAQNPGSSRLVGIRVGRTLMLGWGLAALLGALAGVLVAPRLFLDVNLMG
                     GVLVYAFAGAALGGFDSPLGAVVGSWIIGVTENLAGTYVDAIGADLKVLVPLAVIFVV
                     LLVRPSGLFGSPEVTRA"
     gene            1350..2279
                     /locus_tag="VEP73_00875"
     CDS             1350..2279
                     /locus_tag="VEP73_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT25025.1"
                     /translation="MSLRVVAGGRRHRLLRAAALAATALVAVGAYTAAILTARAQVPY
                     LLTVPAAALVTFALGFAFGVPALRLRGLYLALVTLALSVAVPPLVKRFEGLTGGVQGM
                     SVPQPAVPAWLPLDPDQFLYLLVLAVAAVTCLLAVNLTRGGLGRTLVSIREDERAATT
                     MGVDLAGVKARVFACSAAYAGVAGALFAFAIGFVAPESFTPLLSFSFLAAIVIGGLGT
                     VGGALLGALFIEFVPQYAGDVNQALTGAVYGATLIAVMYLLPGGATGLLRRLGRRLVE
                     VVEAPPPTRQPPGETAMEAGAVSPSGGGGLPAV"
BASE COUNT          227 a          851 c          885 g          376 t
ORIGIN      
        1 gaggggcgcg gcacgttccc ggcgctcacc gtcgacgaga acctccggct cggcgcccac
       61 acccgccggg acacggccgg gatccgcgcc gacctgggcc ggtggtacgg ggtgttcccc
      121 cggctcgccg agcgccgcgg gcagcccgcc ggcagcctgt caggcggcga gcagcagatg
      181 ctcgcgatcg cccgcgcgct gatgctgcgc ccgcggctgc tgctgctgga cgagccgtca
      241 ctgggcctcg cgccgctggt caccagggag ctgttcgagg tgctcgcccg ggtcaagggg
      301 gagcagggca ccaccatggt gctggtggag cagaacgcca gcctcgcgct ggggctcgcc
      361 gaccacgccc acgtgctcga ggccgggcgc gccgtgctcg cgggcccggc agggcggatc
      421 agggcggacg agcacgtgcg gcgcgcctac ctgggctggt gaggagcggc gatgggacgg
      481 tttctccagg tcgtggtcga cgggatcgcc gacggctcca tctacgcgac gctcgcgctt
      541 gccctggtgc tgatcttccg ctcgaccggg atcgtcaact tcgcccaggg cgagatggcg
      601 atgttctcga ccttcgtcgc ctgggggctg gcggacgcgg gctggccgct gtggctcgcg
      661 ctgctcggcg cgctcgcggt ctcgttcctg ggcggcatgg cgatcgagcg ggtgctcatc
      721 cgcccggtgg agggcggccg gccgctcacc atggtgatcg tcaccctcgg gctgttcgtc
      781 ctggtcaact cggcggccgg ctggatctgg gggttctcca accgggggtt ccccaggatc
      841 ttcccggccg gcacggctcg gctcggccag gtcgcgttct cgctcgagtc gctcgggatc
      901 atcgcggtgc tgctcgccgt cgtcgggctg ctctggctcc tgttccagca caccagggcg
      961 gggctggcga tgcgggcggt cgcgcagaac cccggctcga gccgcctggt cgggatccgc
     1021 gtcggccgga cgctgatgct cggctggggg ctggcggcgc tgctcggcgc gctggccggg
     1081 gtgctggtcg cgccgcggct gttcctcgac gtgaacctga tgggcggcgt gctggtctac
     1141 gccttcgccg gcgcggcgct cggcggcttc gactcgccgc tcggcgcggt cgtcgggagc
     1201 tggatcatcg gcgtcaccga gaacctcgcc ggcacctacg tggacgccat cggtgcggac
     1261 ctgaaggtgc tcgtgccgct ggcggtcatc ttcgtggtgc tcctggtgcg cccctcgggg
     1321 ctgttcggct cgccggaggt gacccgcgca tgagcctgcg ggtcgtcgcg ggcggccggc
     1381 ggcaccggct gctgcgcgcc gcggcgctcg ccgcgaccgc gctggtcgcg gtcggcgcct
     1441 acaccgcggc catcctgacc gcaagggcgc aggtgccata cctcttgacc gtgcccgccg
     1501 ccgcgctcgt caccttcgcg ctcggcttcg ccttcggcgt gccggcgctg cgcctgcggg
     1561 ggctgtacct ggcgctcgtc acgctcgcgc tctcggtcgc ggtcccgccc ctggtcaagc
     1621 gcttcgaggg gctcacaggc ggcgtgcagg ggatgtcggt gccgcagccg gccgtgcccg
     1681 cctggctgcc cctcgacccc gaccagttcc tctacctgct ggtgctcgcc gtcgccgcgg
     1741 tgacgtgcct cctcgcggtg aacctcaccc ggggcgggct gggccggacg ctggtctcca
     1801 tccgcgagga cgagcgggcc gcgaccacca tgggcgtcga tctcgcgggg gtgaaggccc
     1861 gcgtgttcgc ctgcagcgcg gcctacgcgg gcgtcgcggg cgcgctgttc gccttcgcca
     1921 tcggcttcgt cgcgccggag tcgttcaccc cgctgctgtc gttcagcttc ctcgccgcca
     1981 tcgtgatcgg cgggctcggc accgtcggcg gcgcgctgct cggcgcgctc ttcatcgagt
     2041 tcgtgccgca gtacgcgggc gacgtcaacc aggcgctcac cggcgccgtc tacggcgcga
     2101 cgctgatcgc agtcatgtac ctgctgcccg ggggcgccac cggcctgctg cggcgcctcg
     2161 gccgccgcct ggtcgaggtg gtcgaggcgc caccgcccac caggcagccg cccggcgaga
     2221 cggccatgga ggccggcgcc gtctcgccct cgggaggcgg tggcctgccg gccgtctgaa
     2281 ggacgtcgtc tggagaaaag ggagggaccc atgctggcac ggaggaggac gctcgtccc
//
LOCUS       DASPYY010000066         6271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_151153, whole genome shotgun sequence.
ACCESSION   DASPYY010000066 DASPYY010000000
VERSION     DASPYY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151153"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VEP73_00880"
     CDS             complement(<1..206)
                     /locus_tag="VEP73_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin"
                     /protein_id="HYT25026.1"
                     /translation="MGTAGLLWAWGPVWLASAAVAQATDRSNAEIVDFHYNPGTTTVA
                     VGTTVTWVNHGSRPHTVTDRGGTF"
     gene            complement(239..679)
                     /locus_tag="VEP73_00885"
     CDS             complement(239..679)
                     /locus_tag="VEP73_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HYT25027.1"
                     /translation="MDLIGLLNDLTGGNLLLWKVVGATAVFFLAGLQVLLAARLWGVT
                     PFPPVSSGAAARAHRVVGRAALLVAVLVAVACIAGPAGPTSPTRVLLHSVFGTLVFVI
                     LAAKFALLRVLRRGDSLLPWIGTALFLTFVGIWATSVADYVTAR"
     gene            complement(692..1078)
                     /locus_tag="VEP73_00890"
     CDS             complement(692..1078)
                     /locus_tag="VEP73_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYT25028.1"
                     /translation="MDARRVLWGAIVVGFLAIAVSGPVAMLRERPAAARPAAASPAGA
                     PATTPVVRMENIAFVPATLTVRPGTKVRFENRDVAPHTVTAATPGGIDSGTINPGRSF
                     ELVVSQPFNYVCTIHPSMKAKILLSG"
     gene            complement(1268..2170)
                     /locus_tag="VEP73_00895"
     CDS             complement(1268..2170)
                     /locus_tag="VEP73_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HYT25029.1"
                     /translation="MGNGAVPRTPINELGLSTGKKARLYRILHQHGLRNGTAMFLPYD
                     QGLEHGPRDFFDNPAAADPRYIIKLAVEGGFNGIAIQIGTAEKFYWDYAGEVPLLLKL
                     NGRTEIPPADAPRSPLNATVEDAARLGADAVGYTLYVGSAQQEADFEAFARVRRDAHR
                     LGLPLVVWSYPRGSSVDAKGGKNSLYEVDYAARTAAELGADVVKVNFPDPTATEGVYK
                     TYGTPVSEQDAVSAVVRSAVRTPVLLSGGERGGDDEVLDKARMAMEAGATGLIFGRNV
                     WQRERDESLRFIARLRELLEKYPS"
     gene            complement(2341..2679)
                     /locus_tag="VEP73_00900"
     CDS             complement(2341..2679)
                     /locus_tag="VEP73_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HYT25030.1"
                     /translation="MHTTDALLERAKTRRGRDELAGTTGLSPRRILEWVNHVDLMRLD
                     GVGPEFADLLEAAGVDSPAELAHRNAANLAARLAELNEAKHLTRRVPGEDVVGRWIAE
                     AGSLPRAVEH"
     gene            2851..3066
                     /locus_tag="VEP73_00905"
     CDS             2851..3066
                     /locus_tag="VEP73_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25031.1"
                     /translation="MIAFLTVALALFFFVMKPVNNLVSLARHREPPDPTTRKCPECLS
                     EIPLQARRCAFCTAEVGAVGPAATAER"
     gene            complement(3099..4445)
                     /locus_tag="VEP73_00910"
     CDS             complement(3099..4445)
                     /locus_tag="VEP73_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HYT25032.1"
                     /translation="MSDQAHDHAHHGPGFASPAEAIAGPREELVYVAAIHTGTGVEEP
                     DFLATVDVAEGSPTYGQIVHRTPMPNVGDELHHFGWQVCSSACHTSDLERRHLVVPGF
                     RSSRVHVVDVGTDPRAPRIAKVVEPEEIKARTGLSAPHTVHCMPGDNVVLSMLGDADG
                     NDGAGFAVLDAETFAVKGRWENGGPTPVWNYDFWYQPRKGYLVSSEWAAPKTFQDGFR
                     LEDVAEGRYGHSIHVWDLGRRAVAQDIDLGPEGMIPLEVRWLHDPDADEGFVGAALSS
                     TMWHVHRDRDGRFAADKVIEVETRDLEGFPVPVPGLITDLVVSMDDRYLYFSNWLHGD
                     LRQYDISDPAHPVLASSVSLGGLFEEARHPNGTRLTGGPQMLQLSMDGKRLYVSNSLY
                     STWDNQFYPTLESWLLKLDAGDGGLRVDPDFFVDFSPARAHEIHLPGGDVTTEIFA"
     gene            4683..4961
                     /locus_tag="VEP73_00915"
     CDS             4683..4961
                     /locus_tag="VEP73_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001000 - bacterial-type RNA polymerase
                     core enzyme binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HYT25033.1"
                     /translation="MNDSRYRLPAEFDRLQAPSNAYAPRQVVTYLCTREHLVSVPFAA
                     DAEVPEMWDCRCGKPATRVAPDHAAAATRVGAEPAARMAAEEPKASTP"
     gene            5042..6220
                     /locus_tag="VEP73_00920"
     CDS             5042..6220
                     /locus_tag="VEP73_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HYT25034.1"
                     /translation="MDASQVWDLIVVGAGPAGSAAALQARLLRPGSAVLLLDKAVFPR
                     DKACGDGIAPHAADELARLGAAHALDGYPPIHRLRIRAPGGGEVAGRPARPARVVPRA
                     VFDARLVEAAVAGGAVLRRERVRTLEERRGLVVVNGELAGRALVGADGANSAVRRLLG
                     VPLNPPGDLAIALRGYAPAGDGPLEQFIGWVPDGWPAYVWSFPTGTGLANVGYGLLRS
                     RFAGGRAELSARLATLLPEARPDEASLRAHHLPFSTHRPRPGRGRVLLAGDAASLVNP
                     LSGEGIYYALASGRLAATAAVLATADPAAAYRQLLARSLGRHFRHSTVLARAIRSRRL
                     ASAAMLASGASPALFDALVEMGLGQARLTAGLVARLPPAYLRARAAGRRTASGPAGRG
                     "
BASE COUNT          801 a         2255 c         2355 g          860 t
ORIGIN      
        1 tcgaacgtcc cgccgcggtc ggtgaccgtg tgcgggcgcg acccatggtt cacccaggtg
       61 acggtcgtgc cgacagcgac cgtggtagtg cccgggttgt agtggaagtc gacgatctcc
      121 gcgttcgacc ggtcggtcgc ctgggcgacc gccgcggagg cgagccagac cggtccccac
      181 gcccacagca ggcccgcggt ccccatgagc gcaatcctga cccctcgtcc catgtccgct
      241 acctcgctgt cacgtagtcc gccacgctcg tcgcccagat gccgacgaac gtgaggaaga
      301 gggccgtgcc gatccacggc agcaggctgt cgcccctgcg gagcacccgc aggagcgcga
      361 acttggccgc caggatcacg aagaccagcg tcccgaacac cgagtgcagc agcacgcggg
      421 tcggcgaggt ggggcccgcc ggaccggcga tgcaggccac ggcgaccagc accgccacga
      481 gcagcgccgc gcggccgacg acccggtgcg ccctggcggc ggccccggag ctcaccggcg
      541 ggaacggcgt caccccccac aaccgcgctg cgagcagcac ctgcagcccg gcgaggaaga
      601 acacggccgt cgcaccgacc accttccaga gcagcaggtt gccgccggtg aggtcgttca
      661 gcaagccgat caggtccacg ccgtcccccc ttcacccgga gagcaggatc ttggccttca
      721 tgctcggatg gatcgtgcac acgtagttga acggctggct gaccaccagc tcgaaggacc
      781 tgcccgggtt gatggtcccg gagtcgatcc ccccgggagt tgcggccgtc acggtgtgcg
      841 gggcgacgtc ccggttctcg aagcgcacct tggtcccggg tcggacggtc agcgtcgccg
      901 gcacgaaggc gatgttctcc atcctgacga ccggcgtggt ggccggcgcc ccggcagggg
      961 aggcggcggc cgggcgggcg gcggccgggc gctccctgag catcgccacc gggcccgaga
     1021 ccgcgatcgc caggaagccg acgacgatgg caccccacag cacacgcctt gcgtccacgc
     1081 gagcccccca cgtcggcagg tcgaccgttg cagccaggag cctggccggc accgcggtac
     1141 gctacggacg acttctaagc gatgcaagcg tccgtgtcca gtacccgcaa tgggaggcgg
     1201 agcggaccag gcggaggcgg cgaccctagg tcggggccgc cgcgcgcccg cctaccctag
     1261 cccaacccta ggacgggtac ttctcgagca gctcgcgcag gcgcgcgatg aagcggagcg
     1321 actcgtcgcg ctcgcgctgc cagacgttgc ggccgaagat cagcccggtc gcgccggcct
     1381 ccatggccat gcgggccttg tcgaggacct cgtcgtcgcc accccgctcg ccgccgctga
     1441 gcagcaccgg ggtccggacc gcggagcgga ccaccgcgct cacggcgtcc tgctcgctga
     1501 ccggggtgcc gtaggtcttg tacacgccct cggtcgccgt ggggtcgggg aagttgacct
     1561 tgacgacgtc ggcgccgagc tcggcggcgg tccgtgccgc gtagtcgacc tcgtagaggg
     1621 agttcttccc gcccttggcg tccaccgagc tgccgcgggg gtacgaccag acgaccagcg
     1681 gcagcccgag gcggtgggcg tcgcggcgga cccgggcgaa cgcctcgaag tcggcctcct
     1741 gctgcgccga gccgacgtag agggtgtagc cgaccgcgtc cgcgccgagc cgggcggcgt
     1801 cctcgacggt ggcgttgagc ggcgagcgcg gcgcgtcggc cggggggatc tcggtgcgcc
     1861 cgttcagctt gagcagcagc ggcacctcgc cggcgtagtc ccagtagaac ttctcggcgg
     1921 tgccgatctg gatggcgatc ccgttgaagc cgccctcgac cgccagcttg atgatgtacc
     1981 tggggtcggc cgccgcgggg ttgtcgaaga agtcgcgggg gccgtgctcg agcccctggt
     2041 cgtagggcag gaacatcgcc gtgccgttcc tcagcccgtg ctggtggagg atccggtaca
     2101 gccgggcctt cttgccggtg ctgagcccga gctcgttgat cggggtgcgg gggaccgcgc
     2161 cgttgcccat gagtgcctcc tggtcgtcgt gggatgcggg cccccttccc gaccgagggc
     2221 cttgcacgtg ccgtcgtccc gggccggcgt gcctgccggc ccggacaccc cattgtccac
     2281 agggcggcgc tgctccggca acctctggcc cgccccggtg cgggacggtg ggcgacctgg
     2341 tcagtgctcg acggctctgg gcagcgaccc ggcctcggcg atccagcgtc cgaccacgtc
     2401 ctcgccgggg acgcggcggg tcaggtgctt ggcctcgttc agctcggcga ggcgggcggc
     2461 caggttggcc gcgttgcggt gggccagttc ggccggggag tccacgccgg ccgcctcgag
     2521 caggtcggcg aactccggtc ccacgccgtc gaggcgcatc aggtcaacgt ggttcaccca
     2581 ctcgaggatg cgcctggggc tgaggccggt ggtgcccgcg agctcgtcac ggcccctgcg
     2641 ggtcttcgcc cgctcgagca gggcgtccgt ggtgtgcacc ccggctgcct ggagcttggc
     2701 ggcccggatg ggccgatgcc ttcgacgtcc tccaccttca tgcgtgctcc tctcagggac
     2761 gggcgccgcc cgtccgcgga cgggcgcgat gcgcgatgtc ggactggtcg ggcaagacct
     2821 gctcacgctg ccgggatcct aaacaacggg gtgatcgcct tcctcacggt cgcgctcgcg
     2881 ctcttcttct tcgtgatgaa gccggtgaac aacctcgtgt cgctcgcccg ccaccgcgag
     2941 ccgccagacc cgaccacacg caagtgcccg gagtgcctga gcgagatccc gctgcaggcg
     3001 cggcgctgcg cgttctgcac ggcggaggtg ggggcggtgg gcccggcggc caccgccgag
     3061 cggtgagccg gccgggcgcc gtcgcggtca ggggcgggtc aggcgaagat ctcggtggtg
     3121 acgtcgccgc caggcaggtg gatctcgtgc gcccgggcgg gggagaagtc gacgaagaag
     3181 tcggggtcga cacgcaggcc gccgtcgccg gcatcgagct tgaggagcca ggactcgagg
     3241 gtcgggtaga actggttgtc ccaggtcgag tagagcgagt tgctcacgta gaggcgcttg
     3301 ccgtccatcg agagctgcag catctgcggg ccgccggtca gccgggtgcc gttcgggtgg
     3361 cgtgcctcct cgaacaggcc gccgagcgac acggagctcg cgaggaccgg gtgggccggg
     3421 tcggagatgt cgtactggcg caggtcgccg tggagccagt tggagaagta gaggtaccgg
     3481 tcgtccatcg agacgaccag gtcggtgatg aggccgggca ccggcaccgg gaagccctcg
     3541 aggtcgcggg tctcgacctc gatcaccttg tcggcggcga agcgcccgtc gcggtcacgg
     3601 tggacgtgcc acatggtcga ggagagcgcg gccccgacga acccctcgtc ggcgtcaggg
     3661 tcgtgcagcc agcggacctc gagcgggatc atgccctcgg ggccgaggtc gatgtcctgg
     3721 gcgaccgcgc gacgcccgag gtcccagacg tggatcgagt gcccgtagcg gccctcggcc
     3781 acgtcctcga ggcggaagcc gtcctggaac gtcttcgggg ctgcccactc ggacgacacc
     3841 aggtagccct tgcggggctg gtaccagaag tcgtagttcc agaccggcgt cgggccgccg
     3901 ttctcccagc ggcccttgac cgcgaacgtc tcggcgtcca ggaccgcgaa gccggcgccg
     3961 tcgttgccgt cggcgtcgcc gagcatcgag aggacgacgt tgtcgcccgg catgcagtgg
     4021 acggtgtggg gggccgaaag gccggtcctg gccttgatct cctcgggctc gacgaccttc
     4081 gcgatccggg gggcacgcgg gtcggtgccc acgtcgacga cgtggacccg cgacgagcgg
     4141 aacccgggca cgacgaggtg gcggcgctcg aggtcggagg tgtggcaggc cgaggagcag
     4201 acctgccagc cgaagtggtg cagctcgtcg ccgacgttcg gcatcggcgt ccggtggacg
     4261 atctggccgt aggtgggcga gccctccgcc acgtcgacgg tggcgaggaa gtcgggctcc
     4321 tcgaccccgg tgcccgtgtg gatggcggcg acgtagacga gctcctccct ggggccggcg
     4381 atcgcctcgg cgggcgacgc gaagccgggc ccgtggtgcg cgtggtcgtg ggcctggtcg
     4441 gacatgggca tcctcctccg ggatggtccg gtcgtggagg ccgccgaggc gctccgtggg
     4501 cgcggtcgcg gcctgccgct cagcgtgtcc cattgcagca gacctcgcgg gaccgggcca
     4561 gggtcgcgtc caggcggtgg accggtgacc ggattcaccg tcttcccgtc gtttgacagc
     4621 ggagcaatac ttgcatcgtc agcccaccgg gcatgccatc cgagcaccca caaggagcag
     4681 cgatgaacga cagccggtac cgccttccgg cggagttcga ccggttgcag gcgccctcca
     4741 acgcctacgc cccacggcag gtcgtcacgt acctgtgcac gcgggagcat ctcgtctcgg
     4801 tcccgttcgc ggccgacgcc gaggtgcccg agatgtggga ctgtcgctgc ggcaaacccg
     4861 ccacccgggt cgccccggac catgccgcag ccgccacccg ggtgggcgcc gagccggccg
     4921 cgcggatggc ggccgaggag cccaaggcct ccacgccgta gcgcggccgc ccgccttgcg
     4981 gcgtggaggc ccgcggccgc gcccctcagc ggacctgcga ccgcccgtgg catgcgggga
     5041 tatggacgcc agccaggtct gggacctgat cgtggtgggc gccggcccgg ccggctcggc
     5101 cgccgccctg caggcgaggc tgctcaggcc gggcagcgcc gtgctgctgc tcgacaaggc
     5161 cgtcttcccg cgcgacaagg cctgtgggga cggcatcgca ccgcacgccg ccgacgagct
     5221 ggcgcggctc ggcgccgcgc atgcgctcga cggctacccg ccgatccacc ggctgcggat
     5281 ccgcgcgccc ggcggaggcg aggtcgcggg ccgcccagcc cgcccggccc gggtggtccc
     5341 ccgcgcggtg ttcgacgccc ggctggtgga ggcggccgtg gccggggggg ccgtcctccg
     5401 gcgggagcgt gtccgcacgc tcgaggagcg ccgaggcctc gtggtggtca acggcgagct
     5461 ggccggccgg gcgctggtcg gtgccgacgg cgccaactcg gccgtgcgtc gcctcctcgg
     5521 cgtccccctc aacccgcctg gcgacctggc catcgcgctg cggggctacg ccccggccgg
     5581 cgacggcccg ctcgagcagt tcatcggctg ggtgccggac ggctggccgg cgtacgtctg
     5641 gtcgttcccg acagggaccg ggctggctaa cgtcggctac ggcctgctgc ggagccgctt
     5701 cgcgggtggc cgggccgagc tgtcggcgcg gctcgccacc ctgctccccg aggcacgacc
     5761 cgatgaggcc agcctgcgag cgcaccacct gccgttctcc acccaccggc ccaggcccgg
     5821 ccgggggcgg gtgctgctcg cgggcgacgc cgcgtcgctc gtcaaccccc tgagcggcga
     5881 gggcatctac tacgccctcg cctcggggcg gctcgcggcc accgcggccg tgctcgcgac
     5941 cgccgacccg gccgccgcct accggcagct cctggccagg tcgctcggcc ggcatttcag
     6001 gcactcgacg gtgctcgcgc gggcgatccg gtcgcggcgg ctcgcgtcgg cggcgatgct
     6061 cgcgtcgggt gcctcgccgg cgctgttcga cgcgctggtg gagatgggcc tcggccaggc
     6121 ccggctgacc gcgggcctgg tcgcgcgcct cccccccgcc tacctgcgcg cccgcgcggc
     6181 gggacggcgg accgcctccg gccccgcggg gcggggctga ccggccgggg ggaggcggtc
     6241 ggccgcggcg gcggcgatgg ccgccaccgc g
//
LOCUS       DASPYY010000067         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_713166, whole genome shotgun sequence.
ACCESSION   DASPYY010000067 DASPYY010000000
VERSION     DASPYY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713166"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..948
                     /locus_tag="VEP73_00925"
     CDS             121..948
                     /locus_tag="VEP73_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018587041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HYT25035.1"
                     /translation="MIGMVLAAGTGRRLRPHTNGRPKALLPVAGETTILDIALRNLAA
                     AGLTEVVIVVGYAAGMVADRIPALERAYGISIDLVHNERAEEWNNAYSLWLAREYFGR
                     GVMLVNGDTVHPVSVEKTLLAASAGGQPARPARLARKDPARYSAGTPDGSRREPLLGR
                     IIIAIDDIKALAEEEMKVVLDGNGMLVQITKVMDPAHAHGEYMGVTLIEPAAAVALAD
                     CLETTWRRDPHLFYEDGFAEFSERGGAVLAAPIGAIEWVEVDDHADLLRAREIAFRC"
     gene            1034..>1675
                     /locus_tag="VEP73_00930"
     CDS             1034..>1675
                     /locus_tag="VEP73_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYT25036.1"
                     /translation="MPLLARMLAAPLVIDVRPGAIAGLAALLTDRKIVSDGRVAIAVG
                     PGQGDAIVGALPPGLGDVIKLPDGGYDSAVGLGRDLRAGRYEAVAGIGGGKTIDVTKL
                     AAHVAGIPMVAVATSLAHDGIASPVSTLAHESGSGSYGVVPPVAVVVDLDRVRRAPGP
                     LSTAGIGDAISNLSAVADWELSAADTGEAVDGLAAAMARSAAHAVLHEPGPGNT"
BASE COUNT          239 a          601 c          610 g          225 t
ORIGIN      
        1 cctcgccgcc acgttcggcc tggcgacgtt cgggtacgtt gggctcgcag cgtatcttgg
       61 tgtgctcatc tgccggaagg tcgtgaccgg ctacctcgta cccagggagg aagaccggca
      121 gtgatcggta tggttctcgc cgcgggaacg ggccgcaggc tccgcccgca cacgaacggc
      181 cggccgaagg ccctgctgcc ggtggcaggc gagaccacca tcctcgacat cgcgctgcgc
      241 aacctggccg ctgcaggcct gaccgaggtc gtgatcgtcg tcggctacgc ggccgggatg
      301 gtcgccgacc ggatcccggc gctcgagcgg gcctacggca tctcgatcga cctcgtgcac
      361 aacgagcgcg ccgaggagtg gaacaacgcc tactcgctgt ggctcgcccg cgagtacttc
      421 gggcggggcg tcatgctcgt caacggcgac accgtgcacc cggtcagcgt ggagaagacg
      481 ctcctcgccg ccagcgcggg cggccagccc gccaggccgg cccgcctcgc gcgcaaggat
      541 ccggccaggt acagcgcggg gacacccgac gggtcgcggc gcgagcccct gctcggccgg
      601 atcatcatcg ccatcgacga catcaaggcc ctggccgagg aagagatgaa ggtcgtcctc
      661 gacggcaacg ggatgctcgt gcagatcacc aaggtgatgg acccggcaca cgcgcacggc
      721 gagtacatgg gcgtcaccct gatcgagccg gccgcggccg tcgccctcgc cgactgcctg
      781 gagacgacct ggcggcgcga cccgcatctg ttctacgagg acggcttcgc cgagttctcc
      841 gagcggggcg gcgcggtgct cgccgcgccc atcggcgcca tcgagtgggt cgaggtcgac
      901 gaccacgccg acctgctacg ggccagggag atcgccttcc gctgctaatc tcgcgctagg
      961 ttccgcaccg ctgcgccggg cggatccgcg gatcggcccg cgtccgcatc gccgggctgg
     1021 ccagaggagg atcatgccgc tgctggcgcg aatgctcgcc gccccgctgg tcatcgacgt
     1081 caggccgggg gcgatcgctg ggctcgccgc gctgctcacc gaccgcaaga tcgtcagcga
     1141 cgggcgcgtg gccatcgcgg tcggcccagg tcaaggtgat gccatcgtcg gcgcgctgcc
     1201 gccggggctc ggcgacgtga tcaagctccc tgacggcggc tacgacagcg cggtcgggct
     1261 cggccgggac ctgcgagccg gccggtacga ggcggtggcc gggatcggcg gcggcaagac
     1321 gatcgacgtc accaagctcg ccgcgcacgt ggcgggcatc ccgatggtcg cggtcgcgac
     1381 gagcctggcc cacgacggca tcgcctcccc ggtgagcacg ctcgcgcacg agtcaggcag
     1441 cggctcctac ggcgtggtgc cgccggtcgc ggtcgtggtc gatctggacc gggtgcgccg
     1501 ggcccccggg ccgctctcga ccgccgggat cggcgacgcc atcagcaacc tgtccgccgt
     1561 ggccgactgg gaactgtcag cggccgacac cggtgaggca gtcgacgggc tcgcggccgc
     1621 gatggcgcgg tcggcggcac acgcggtgct gcacgagccg ggtcctggca acacc
//
LOCUS       DASPYY010000068         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2117773, whole genome shotgun sequence.
ACCESSION   DASPYY010000068 DASPYY010000000
VERSION     DASPYY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117773"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VEP73_00935"
     CDS             complement(<1..539)
                     /locus_tag="VEP73_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYT25037.1"
                     /translation="MRGAVDFLLTGAGQLVTGDPWLGAGPLGLVERGAVAARAGTVVW
                     SGPERALPDLPLAAGATRLDAGGRAVVPGFVDAHTHLVFAGDRAEEFAARLRGVGYEE
                     ALAAGGGINRTVRETRAATTTQLEAAAAGRLVRAALHGTTTLEAKSGYGLTVADERRT
                     LEVVAALGARARVDVVPTFL"
     gene            complement(536..>2194)
                     /gene="hutU"
                     /locus_tag="VEP73_00940"
     CDS             complement(536..>2194)
                     /gene="hutU"
                     /locus_tag="VEP73_00940"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYT25038.1"
                     /translation="TPIRAARGTQLSARGWQQEAALRMLCNNLDPDVAERPDDLVVYG
                     GTGRAARDWRSFDRIVATLRRLRDDETLLVQSGKPVGVFRTHSFAPRVLIANSLLVPE
                     WATWEEFRELEAAGLTMYGQMTAGSWIYIGTQGILQGTYETFAALAAARGWPSLAGRI
                     VLTAGLGGMGGAQPLAVTMNEGVALCVEVDPEHLERRLATGYLDERADDLADAVERVE
                     RAARERRAVSVGVLGNAADVLPELLAAGFRPDVVTDQTSAHDPLGGYVPSGVDPGDAP
                     AARRDDPAGYVKQARESMAAHCQAMVGLLRAGAEVFDYGNNLRGEARAAGFADAFAYP
                     GFVPAYIRPLFCEGKGPFRWAALSGDPADIARTDQAVLGLFGRDEHLARWLRLASERV
                     RFQGLPARICWLGYGERHQAGLAFNELVAKGELRAPIVIGRDHLDAGSVASPYRETEA
                     MADGSDAIADWPILNALLNTASGASWVSVHHGGGVGIGRSIHAGVVVVADGTAEGARR
                     VELVLTNDPGTGVMRHADAGYAIATGVAHERGLDLPMVTAGGSG"
BASE COUNT          266 a          901 c          772 g          255 t
ORIGIN      
        1 aggaacgtgg gcaccacgtc cacgcgggcg cgggcgccga gcgcggccac gacctcgagc
       61 gtgcgccgct cgtcggccac ggtcaggccg tacccggact tcgcctcgag cgtcgtggtc
      121 ccgtggagcg ccgcccgcac caggcgcccg gccgcggccg cctcgagctg cgtggtggtc
      181 gcggccctcg tctcccgcac cgtccggttg atgccgccgc ccgccgccag cgcctcctcg
      241 tagccgacgc cgcgcagccg ggcggcgaac tcctcggccc ggtcgcccgc gaacaccagg
      301 tgggtgtggg cgtccacgaa gccgggcacg accgcgcgcc ctccggcgtc caggcgggtc
      361 gcccccgccg cgagcggcag gtcgggcagg gcccgctccg gccccgacca caccaccgtc
      421 ccggccctgg ccgccaccgc gccccgctcg accagcccga gcgggccggc gccgagccag
      481 gggtcgcccg tgacgagctg cccggccccg gtgagcagga agtcgaccgc ccccctcacc
      541 cggacccccc ggcggtcacc atgggcaggt cgaggccgcg ctcgtgggcc acgccggtcg
      601 cgatggcgta gccggcgtcg gcgtggcgca tcaccccggt gcccgggtcg ttggtgagga
      661 ccagctcgac gcggcgtgcc ccctccgcgg tgccgtcggc gacgaccacc accccggcgt
      721 ggatcgaccg cccgatcccc accccgccgc cgtggtggac gctgacccac gacgccccgg
      781 aggcggtgtt cagcagcgcg ttcaggatcg gccagtccgc gatcgcgtcc gacccgtcgg
      841 ccatcgcctc ggtctcccga tacggcgagg cgaccgagcc ggcgtccagg tggtcgcggc
      901 cgatcacgat cggcgcccgc agctcgccct tggcgaccag ctcgttgaac gccaggccgg
      961 cctgatggcg ctcgccgtag ccgagccagc agatccgcgc gggcaggccc tggaagcgca
     1021 cccgctcgct ggccaggcgc agccagcgag ccagatgctc gtcgcggccg aacagcccga
     1081 gcaccgcctg gtcggtgcgg gcgatgtcgg ccgggtcgcc ggacagcgcc gcccaccgga
     1141 acgggccctt cccctcgcag aacagcggcc ggatgtaggc gggcacgaac cccgggtacg
     1201 cgaacgcgtc ggcgaacccg gccgcgcgcg cctccccccg caggttgttg ccgtagtcga
     1261 agacctcggc gcccgcccgc aggaggccga ccatggcctg gcagtgcgct gccatcgact
     1321 ccctggcctg cttcacgtac ccggccgggt cgtcgcgcct ggccgccggg gcgtcgcccg
     1381 ggtcgacccc gctcggcacg tacccgccga gcgggtcgtg cgccgaggtc tggtcggtga
     1441 ccacgtcggg ccggaacccg gccgcgagca gctcagggag cacgtcggcc gcgttgccga
     1501 gcacgccgac ggagaccgcc cggcgctcgc gggcggccct ctcgacccgc tccaccgcgt
     1561 cggcgaggtc gtcggcccgc tcgtcgaggt acccggtcgc gagccggcgc tcgaggtgct
     1621 ccgggtccac ctcgacgcac agcgccacgc cctcgttcat cgtcaccgcc agcggctgcg
     1681 ccccgcccat cccgccgagc cccgcggtga gcacgatcct gccagcgagg ctcggccagc
     1741 cccgcgccgc ggccagcgcc gcgaaggtct cgtaggtgcc ctgcaggatc ccctgcgtcc
     1801 cgatgtagat ccacgacccg gccgtcatct gcccgtacat cgtgaggccg gccgcctcga
     1861 gctcccggaa ctcctcccag gtcgcccatt ccgggacgag cagggagttg gcgatcagca
     1921 ccctgggcgc gaacgagtgg gtccggaaca cccccaccgg cttccccgac tgcaccagca
     1981 gcgtctcgtc gtcgcggagc cggcgcagcg tcgccacgat ccggtcgaac gacctccagt
     2041 cccgcgccgc ccgacccgtc ccgccgtaga cgaccaggtc gtcggggcgc tccgcgacgt
     2101 cggggtccag gttgttgcag agcatgcgca gcgcggcctc ctgctgccag ccgcgagcgc
     2161 tcagctgggt gccgcgtgcg gcgcggatcg gggt
//
LOCUS       DASPYY010000069         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2258684, whole genome shotgun sequence.
ACCESSION   DASPYY010000069 DASPYY010000000
VERSION     DASPYY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258684"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEP73_00945"
     CDS             <1..744
                     /locus_tag="VEP73_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYT25039.1"
                     /translation="PGSTFKVITSAALISHGVTPRSAASCPGTATVDGEVFHNAESEA
                     PVSNLLQAFTELCNTAFIKLATSHLSPSDLPSAAQLLGLGGKPDIGLVAFGGSVPQPR
                     DEADLAATSIGQGRVLVSPLAMAMVAAAADTGTVRAPRLVASVASEVGSVITGSPARQ
                     LPAPLVRDLHEMMASVVASGTAAGQGLPSGTYAKTGTAQYGTTKPLKLDAWLMGFRGN
                     IAFAALVVDSAGNGGPTCGPIVARFLRGL"
     gene            complement(759..1760)
                     /locus_tag="VEP73_00950"
     CDS             complement(759..1760)
                     /locus_tag="VEP73_00950"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013138797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYT25040.1"
                     /translation="MLPVAELHVHIEGTLEPELLVKLAVRNGTWMPTLDVAELRTTYR
                     FDSLSSFLSLYYANLQVLKVEADFYDLASDYLTRAAAAGVRRAEIFFDPQTHLANGVP
                     LEEVFGGLTAALADGARDHGISAVLILCFLRDLGADAADAVLRACLPFRDTFIGVGLD
                     SAEVGYPPALFAQVYRRAAAEGLRLVAHAGEEGGPDYVREALDELHVERVDHGVRSME
                     DPELVARLCAEQIPLTVCPLSNVALRVVRTLGDHMLPGMLDAGLLATVNSDDPAYFGG
                     YVDDNLAALRGAFGYDNARLATLARNSFDACFAPEADKRKWKAEVGSWLAARAAAPS"
     gene            complement(1789..>2031)
                     /locus_tag="VEP73_00955"
     CDS             complement(1789..>2031)
                     /locus_tag="VEP73_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HYT25041.1"
                     /translation="ELPEDRDDDDEADEYLDGLLLDDGPDPRAGRLVAEDEGAHADDE
                     EDLVARDVGIDGGAASAEEAAVHVVEDDDTDIRSGD"
BASE COUNT          273 a          741 c          725 g          292 t
ORIGIN      
        1 ccgggctcga cgttcaaggt gatcacgtcc gccgcgctga tcagccacgg ggtgaccccg
       61 cggtcggcgg ccagctgccc gggcacggcc accgtcgacg gcgaggtgtt ccacaacgcc
      121 gagagcgagg ctccggtgag caacctgctg caggcgttca ccgagttgtg caacaccgcc
      181 ttcatcaagc tggctaccag tcacctgtcg ccatcggacc tcccgtccgc ggcgcagctg
      241 ctcgggctcg gcggcaagcc tgacatcggc ctggtcgcct tcggcggctc ggtcccgcag
      301 ccgcgtgacg aggccgacct ggccgccact tccatcggcc aggggcgggt gctggtctcg
      361 ccgctggcca tggcgatggt cgccgcggcc gccgataccg gcacggtgcg cgcaccgcgg
      421 ctggtggcga gcgtggcgag cgaggtcggc tcggtcatca ccggctcgcc ggcccgtcag
      481 ctgcccgcgc cgctcgttcg cgacctgcac gagatgatgg cctcggtggt ggccagcggc
      541 accgcggccg gccagggcct gccgagcggt acctacgcca agaccgggac cgcgcagtac
      601 gggacgacca agccgctgaa gctcgacgcc tggctgatgg gtttccgggg caacatcgcg
      661 ttcgccgcgc tcgtggtcga ctcggccggg aacggcgggc cgacctgcgg cccgatcgtg
      721 gcgaggttcc tcagggggct ctagcccggc gcgcggtgct agctcggcgc cgcggcgcgg
      781 gccgcgagcc agctgccgac ctcggccttc cacttccgct tgtccgcctc gggcgcgaag
      841 caggcgtcga acgagttccg ggcgagcgtg gcgagacgcg cgttgtcgta tccgaacgcg
      901 ccgcgcagcg cggccaggtt gtcgtccacg tatccgccga agtaggcagg gtcgtcgctg
      961 ttcacggtgg cgagcaggcc cgcgtccagc atgccgggca gcatgtggtc gccgagcgtg
     1021 cggaccactc gcagcgccac gttggacagc gggcagacgg tcagcgggat ctgctcggcg
     1081 cacagcctgg cgaccagttc ggggtcctcc atcgaccgga ccccgtgatc gacccgctcc
     1141 acatgcagtt cgtcgagcgc ctcgcgcacg tagtcagggc cgccttcctc gccggcatgg
     1201 gccaccagcc gcagcccttc ggccgccgcc cgccggtaga cctgggcgaa cagggcgggc
     1261 gggtagccga cctccgcgga gtcgagcccg acgccgatga acgtgtcccg gaacggcagg
     1321 caggcgcgga gcaccgcgtc ggcggcgtcc gcgcccaggt cgcgcaggaa gcacaggatc
     1381 aggacggcgg agatcccgtg gtcccgggcg ccatcggcca gcgccgccgt cagcccgccg
     1441 aacacctcct ccagcggcac gccgttggcc aggtgcgtct gcgggtcgaa gaagatctcc
     1501 gcccgccgca ccccggccgc ggccgcccgg gtgagatagt cgctggccaa atcgtaaaag
     1561 tccgcctcga ccttcagcac ctgcaggttg gcgtagtaca ggctcaggaa ggacgacagg
     1621 ctgtcgaagc gatacgtggt ccgtagctcg gccacgtcca gggtcggcat ccaggtgccg
     1681 ttgcgcaccg cgagcttcac gagcagctcg ggttccagcg tgccctcgat gtggacgtgc
     1741 agctcggcga ctggcagcat gcgacccctc accggaccga tcccgccgtc agtcgccgga
     1801 gcggatgtcg gtgtcgtcgt cctcgaccac gtggaccgcg gcttcctcgg cgctggcggc
     1861 cccgccgtcg atgccgacgt cgcgggcgac gagatcctcc tcgtcgtcgg catgggcgcc
     1921 ttcgtcctcg gccaccagcc ggcccgcccg cgggtccggg ccgtcgtcga gcaggaggcc
     1981 gtcgaggtac tcgtcggctt cgtcgtcgtc gtcgcggtct tcgggcagct c
//
LOCUS       DASPYY010000070         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1275826, whole genome shotgun sequence.
ACCESSION   DASPYY010000070 DASPYY010000000
VERSION     DASPYY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275826"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VEP73_00960"
     CDS             complement(<1..451)
                     /locus_tag="VEP73_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYT25042.1"
                     /translation="MSEQAPFSWPHVLGPLLRCEDLTAAQAAAAMRIILAGEATPAQI
                     AAFATGLRIKGETATEVAGLVQAMLEAAAPLDVPGPLLDTCGTGGDRAGTFNVSTLAA
                     VVAVGAGQRVAKHGNRAASGRCGSADLLEGLGVAIALPPEGVAATIAE"
     gene            complement(527..1699)
                     /locus_tag="VEP73_00965"
     CDS             complement(527..1699)
                     /locus_tag="VEP73_00965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYT25043.1"
                     /translation="MKLADRMGRLGTESAFEMLARARGLEATGREIIHLEIGEPDFDT
                     APHIKAAAEEALERGHTHYVPAPGIMPVREAVAAFLERTGRLATSPDRVILTPGAKPI
                     MFFTILALCQEGDEVLYPDPGFPMYESIAAFAGATPVPVPLREDHDFRMDPDELASLV
                     TDRTRLVVLNSPQNPCGSALTRADVEAIAQVALEHDLVVLSDEVYWAIAYGGTHASVL
                     DVDGMAERTVLLDGWSKTFAMTGWRLGFGVFPPALVEPVTRLVINSVSCTSAFSQFAA
                     IAALEGPWDPVTAMVEEFRRRRDVIVAGLNAVPGVSCVEPGGAFYVFPRVTDLGLPAA
                     DVAELLLQRAGVACLPGTAFGAHGEGYLRFSYAASVGDIRRALDAVGSVASELAPA"
     gene            1898..>2138
                     /locus_tag="VEP73_00970"
     CDS             1898..>2138
                     /locus_tag="VEP73_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HYT25044.1"
                     /translation="MPTSQAPPRPGYRDGARAAAPLAVADLAFGTSFGVLAHGAGFGN
                     AAAVVMSATTFAGSAQIAATSVLATGGGVAAAVTAA"
BASE COUNT          291 a          781 c          797 g          269 t
ORIGIN      
        1 tctccgcgat cgtcgccgcc accccctcgg gtggcagcgc gatcgccacc ccgagcccct
       61 cgagcaggtc ggcgctcccg cagcgcccgg aggcggcgcg gttgccgtgc ttggccaccc
      121 gctgcccggc accgaccgcg accaccgcgg cgagcgtgga cacgttgaag gtgcccgccc
      181 ggtcgccgcc cgtcccgcag gtgtcgagca gcggcccggg gacgtcgagc ggcgcggcgg
      241 cctccagcat ggcctggacg agcccggcca cctccgtggc ggtctccccc ttgatgcgca
      301 ggccggtcgc gaaggcggcg atctgcgccg gcgtggcctc gccggcgagg atgatccgca
      361 tggctgcggc cgcctgcgcc gcggtgaggt cctcgcagcg cagcaggggc ccgagaacgt
      421 gcggccagga gaaaggggct tgctcggaca cgtggggctc ccggttcatc gaggcatgcg
      481 cggctgccaa gcctaccggg aacgggggcg gccgggcgcc tcccggtcag gccggggcga
      541 gctcggaggc gaccgacccg accgcgtcga gggcgcggcg gatgtcgccc accgaggcgg
      601 cgtaggagaa gcggaggtag ccctcgccgt gggcgccgaa ggccgtgcct ggcaggcacg
      661 cgacgccggc ccgctggagg agcagctcgg cgacgtcggc cgccgggagc ccgaggtcgg
      721 tcacgcgcgg gaacacgtag aaggcgcctc ccggctcgac gcagctgacg ccagggacgg
      781 cgttcaggcc ggccacgatg acgtcccggc gccggcggaa ctcctcgacc atcgcggtga
      841 ccgggtccca cgggccctcc agggcagcta tggccgcgaa ctggctgaac gcggacgtgc
      901 aggacaccga gttgatgacg aggcgggtca ccggctcgac cagggcgggc gggaagacgc
      961 cgaacccgag gcgccagccg gtcatcgcga acgtcttgga ccaaccgtcg agcaggaccg
     1021 tccgctcggc catcccgtcg acgtcgagca cgctcgcgtg cgtcccgccg taggcgatcg
     1081 cccagtacac ctcgtcggac agcaccacga ggtcatgctc cagcgccacc tgggcgatcg
     1141 cctcgacgtc ggcgcgggtg agcgcgctgc cgcacgggtt ctgcggcgag ttcagcacga
     1201 cgaggcgggt gcggtcggtg accagcgagg cgagctcgtc ggggtccatg cggaagtcgt
     1261 ggtcctcgcg gaggggcacc ggcaccgggg tcgcgccggc gaacgcggcg atcgactcgt
     1321 acatcgggaa gccggggtcc gggtagagga cctcgtcgcc ctcctggcag agcgcgagga
     1381 tggtgaagaa catgatcggc ttggcccccg gggtgaggat cacccggtcg ggcgaggtgg
     1441 cgaggcgccc ggtccgctcg aggaacgcgg ccaccgcctc ccgcaccggc atgatgccgg
     1501 gcgctggcac gtagtgggtg tgcccccgct cgagcgcctc ctcggcggcg gccttgatgt
     1561 gcggggccgt gtcgaagtcg ggctcgccga tctcgaggtg gatgatctcg cggccggtcg
     1621 cctcgaggcc gcgcgccctg gccagcatct cgaacgccga ttcggtcccg agccgcccca
     1681 tccggtccgc gagcttcact gctggcccct tctgcgaggc tgtccggtct gtaccggagc
     1741 ataggcgcca ccgcggaggg cggtaaaccg gccgggagga ggcaccgtgg tcgtgacgtc
     1801 atgccccaag ccccttcacc ggtttcgaat caccgtcata gggtagacaa tggtgacgca
     1861 agaccgagtg acctcgttcc tcaggaggct gtcgaccatg ccgacgagtc aagcaccccc
     1921 gcgacccggc taccgggacg gcgcgcgtgc cgccgccccg ctcgccgtcg ccgacctcgc
     1981 cttcggcacc tcgttcggcg tcctcgccca cggcgcgggc ttcggcaacg ccgccgcggt
     2041 cgtcatgtcg gccaccacct tcgcgggctc cgcccagatc gcggccacct ccgtcctcgc
     2101 gaccggcggc ggggtcgcgg ccgcggtgac cgccgcgc
//
LOCUS       DASPYY010000071         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2680758, whole genome shotgun sequence.
ACCESSION   DASPYY010000071 DASPYY010000000
VERSION     DASPYY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680758"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..739)
                     /locus_tag="VEP73_00975"
     CDS             complement(89..739)
                     /locus_tag="VEP73_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25045.1"
                     /translation="MFPFGPAGSPDELLEVPGCPVCLALPQIETELVGWFALQALHDP
                     DSRRRVLAARGLCPRHWRLVAAEEQHRRQSMLGTAELFAAVLSRQGTHEETAVNCPVC
                     ADLAASARHRLYLLLVDLGQERLDAAPATWRPCLPHLRGLGELRLERWLQRWAHEREE
                     RILTDAVAAARRYVRTRQQRYHAEATGTEAQDLVDAMAALLGDGPPAAQPDDRAVR"
     gene            complement(742..933)
                     /locus_tag="VEP73_00980"
     CDS             complement(742..933)
                     /locus_tag="VEP73_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25046.1"
                     /translation="MSVTTPPDDRDDGRRKIASTLAHAERELLALLAENRAEPQLTSL
                     ADRVEELEDILRPVIEAVR"
     gene            complement(911..1366)
                     /locus_tag="VEP73_00985"
     CDS             complement(911..1366)
                     /locus_tag="VEP73_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYT25047.1"
                     /translation="MARTAVANMVVNLCPECFTRLDGTPPRCPACAADLAETGPYDYE
                     QGLIRALRHPFHDQRVLAARILGKRRSRVAVPSLVAVVEDGGRDPYLVAEAVRALARI
                     GDDRGDGGRPSRRGRRARRRAGRRGKSSPRAGIRPHGSEGSQHVCHDAA"
     gene            complement(1407..>1909)
                     /locus_tag="VEP73_00990"
     CDS             complement(1407..>1909)
                     /locus_tag="VEP73_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25048.1"
                     /translation="AAAAALLGLAAWPVAVDARPRPLLVLLGTVAALLQLAGLAGSLP
                     ALVPWSIGTLMASYTLGLLWRGGGVDASAPLYGGGLLLAAELSYWSIELRRVRALPGP
                     VGRRRAAVVAALVLAGIALAATSTLVAGPLPVRSTLLLRAVAVVAAVATVATVAGLAR
                     RGRAQR"
BASE COUNT          261 a          697 c          745 g          206 t
ORIGIN      
        1 gggcgccaag gaccccgccg gcgaagatcg cggcgagctc ccgccagtca gctctgggca
       61 cgagcgcgct ccggtcgcgg ggccgagctc atcgcacggc tcggtcatcg ggctgggcgg
      121 caggcgggcc gtcgccgagc agggccgcca tcgcgtcgac gaggtcttgc gcctcggtgc
      181 cggtcgcctc ggcgtgatag cgctgctgac gggtgcggac atagcggcgg gcggcggcga
      241 cggcgtcggt caggatgcgc tcctcgcgct cgtgcgccca ccgctgcagc cagcgctcga
      301 gacgcagctc ccccagccct cgcaggtgcg gcagacacgg ccgccaggtg gccggggccg
      361 catcgagccg ctcttggccg aggtcgacga gcagcaggta gagccggtgc ctggccgagg
      421 cggccaggtc cgcgcagacc gggcagttga cagcggtttc ctcgtgcgtc ccctggcggg
      481 agaggaccgc ggcgaacagc tcggcggtcc ccagcatgga ctggcgccgg tgctgctcct
      541 ccgcggcgac cagccgccag tgccgcgggc acagcccgcg ggccgccagg acccgcctgc
      601 ggctgtcggg gtcgtgcagg gcctgcagcg cgaaccagcc gaccagctcc gtctcgatct
      661 gcgggagcgc caggcagacc ggacacccgg ggacctcgag cagctcgtcg ggtgagccag
      721 ccgggccgaa ggggaacatc ctcagcggac cgcctcgatg accggcctca ggatgtcctc
      781 caattcctcg acacggtcgg cgaggctcgt gagctgcggc tcggcccggt tctcggcgag
      841 gagcgcgagc agctcccgtt ccgcgtgggc cagggtcgaa gcgatcttgc gccggccgtc
      901 gtcccggtcg tcaggcggcg tcgtgacaga catgctggct cccttcactg ccgtgcggac
      961 ggatcccagc cctcggcgag gacttcccgc gacggcccgc gcgacggcgg gcccgtcggc
     1021 cgcgacgcga cggacgacct ccatcccccc ggtcgtcgcc gatcctcgcg agcgcccgta
     1081 ccgcctccgc gacgaggtat gggtcgcgcc cgccgtcctc cacgaccgcg acgaggctgg
     1141 ggaccgccac ccgggaccgc cgcttcccga ggatccgggc ggcgagcacc cgctggtcgt
     1201 ggaaggggtg gcggagggct cggatcaggc cctgctcata gtcgtagggc cccgtttcag
     1261 cgaggtcggc ggcgcatgcc gggcagcgag ggggcgtgcc gtccagccgg gtgaagcact
     1321 cggggcagag gttcacgacc atgttcgcca ccgcggtgcg agccatcgct ggagcgctgc
     1381 ggcgccccga agggaggatg cccaggtcat cgctgcgctc ggccacggcg ggcgagtccg
     1441 gcgacggtcg cgaccgtggc gacggcggcg acgacggcga ccgcccgcag gagcagggtg
     1501 gagcggaccg gcagcggacc cgcgacgagc gtgctggtgg cggccagggc gatcccggcg
     1561 aggacgagtg cggcgaccac ggccgcgcgg cgccgtccca cggggccagg gagcgccctg
     1621 acccggcgga gctcgatcga ccagtacgac agctccgcgg ccagcaggag gccaccgccg
     1681 tagagcggcg cggaggcgtc cacgccgccg ccgcgccaga ggagaccgag ggtgtaggac
     1741 gccatcagcg tcccgatcga ccacggcacg agcgctggca gcgaaccggc caggccggcg
     1801 agctgcagca gcgccgcgac cgtgccgagc agcacgagca gcgggcgcgg ccgggcgtcg
     1861 acggcgaccg gccaggcggc gaggccgagg agcgccgcgg ccgccgccg
//
LOCUS       DASPYY010000072         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_152627, whole genome shotgun sequence.
ACCESSION   DASPYY010000072 DASPYY010000000
VERSION     DASPYY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152627"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VEP73_00995"
     CDS             <1..1410
                     /locus_tag="VEP73_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYT25049.1"
                     /translation="EVPAMLAGRGGVLVQRVVELVRAIEQGRRACSPANVGLLAAHER
                     LERLGRPLNAACAAIGAPDRARTGPLAGRPVGVKDIIAVVGVPTRCGSPASDPRPAAG
                     DAVLVRRLREAGAEVFATTQCLEYAAGFAHPEVGDTRNPRDPSRTSGGSSGGSAALVA
                     AGVCDLALGTDTGGSIRIPAAYCGVVGLKPSYGLLPLEGVFPLSPTCDHAGTLTATVA
                     GAADLLAALTEPSAPTTPSDLTTPSGPATPSDPPDPIAPAGPATFTVGVLAAQLADPS
                     VTPAVRDAVRGALAALGAAGWRVTELTAPWLGELAAWEEVLAVIVAREAYLVHQVRDT
                     SRYAEGTRALLEYGASVSDGRYAAAIERRAELAAAIEACLADVDVLAGPTVGYQAPEQ
                     DPPFGTGEDHGESRFTGPYNLTGHPAVSLPVPTAGSPADRAALPAGLQLVGRRGADVA
                     LLRVAAAAERLIAAPTSPT"
     gene            1428..>1716
                     /locus_tag="VEP73_01000"
     CDS             1428..>1716
                     /locus_tag="VEP73_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HYT25050.1"
                     /translation="MPTTTRRHALTLAGLEVPVVELAGAGDGPRLTVLAGVHGCEYAS
                     IAGVRQWARSLEERELRGRVVAVPVLNLPAFRARSPFVVPDDGKNLNRCFPG"
BASE COUNT          183 a          706 c          618 g          209 t
ORIGIN      
        1 gaggtgcccg ccatgctcgc cgggcgaggc ggcgtcctgg tccagcgggt cgtcgagctc
       61 gtgcgggcca tcgagcaggg gcggcgcgcg tgctcgccgg cgaacgtcgg cctgctggcc
      121 gcccacgagc ggctggagcg gctcggccgg ccgctcaacg cggcctgcgc ggcgatcggc
      181 gccccggacc gggcccgcac cgggccgctg gccggacgcc cggtcggcgt caaggacatc
      241 atcgcggtgg tcggcgtgcc gacccgctgc ggcagcccgg ccagcgaccc ccggccggcg
      301 gccggggacg cggtcctggt ccgccgcctg cgcgaggccg gggccgaggt gttcgcgacc
      361 acccagtgcc tggagtacgc ggcggggttc gcacaccccg aggtcggcga cacgcgcaac
      421 ccccgcgacc cctcccgtac ctcgggcggc tcctccggcg ggtcggcggc cctcgtcgcg
      481 gccggcgtgt gcgacctggc cctcggcacc gacaccggcg gctcgatccg gattcccgcg
      541 gcgtactgcg gggtcgtcgg gctcaagccg agctacgggc tgctcccgct ggagggagtc
      601 ttcccgctct cgccgacctg cgatcacgcg ggcacgctca ccgccaccgt ggccggcgcc
      661 gcagacctgc tagctgccct caccgagccc tccgccccta ccacgccctc cgacctcacc
      721 acgccctccg gccccgccac tccctccgac ccccctgacc ccatcgcgcc tgcgggcccc
      781 gcgaccttca cggtcggcgt cctcgccgcc cagctggcgg acccgtcggt cacgccggcg
      841 gtccgtgacg ccgtccgcgg cgcgctggcc gcgctcggcg cggccggctg gcgggtcacc
      901 gagctgaccg cgccgtggct cggtgagctg gccgcctggg aggaggtcct ggccgtcatc
      961 gtggcccggg aggcgtacct ggtgcaccag gtacgcgaca ccagccgcta cgccgagggc
     1021 acgcgggcgc tcctggagta cggcgcctcc gtcagcgacg gccgctacgc cgccgcgatc
     1081 gagcggcggg cggagctcgc cgctgcgatc gaggcctgcc tcgcggacgt cgacgtcctg
     1141 gccggcccca cggtcggcta ccaggccccg gaacaggatc cgccgttcgg gaccggtgag
     1201 gaccacgggg aaagccggtt caccggcccg tacaacctca ccggccaccc ggccgtgtcg
     1261 cttcccgtgc cgaccgcggg ttcccccgct gatcgcgctg ccctcccggc cggcctccag
     1321 ctggtcggcc gccgcggcgc cgacgtggcc ctgctccggg tggccgccgc cgccgagcgc
     1381 ctgatcgcag ccccgacctc gccgacctag ccatggagga ccgacgcatg cccacgacca
     1441 cgcgccgtca cgcgctcacg ctggccggcc tggaggtacc cgtcgtcgag ctggccggcg
     1501 ccggcgacgg cccgcggctc accgtcctgg ccggagtgca cggctgtgag tacgcgtcga
     1561 tagccggcgt ccgccagtgg gcccggagcc tcgaggaacg ggaactgcgc ggccgggttg
     1621 tcgcggtgcc ggtgctgaac ctgcccgcgt tccgcgcccg cagcccgttc gtcgtgcccg
     1681 acgacggcaa gaacctcaac cggtgcttcc cgggcg
//
LOCUS       DASPYY010000073         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2259910, whole genome shotgun sequence.
ACCESSION   DASPYY010000073 DASPYY010000000
VERSION     DASPYY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259910"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2580
                     /locus_tag="VEP73_01005"
     CDS             <1..>2580
                     /locus_tag="VEP73_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HYT25051.1"
                     /translation="LTEATEPERPGRDPDELDRQADELREEEETLNERLTAGREVLAV
                     ATAGRTAAERELAAAERRQAEGAKAAAARAGRVARLREQVGAADSRTVAAQEEMDRLE
                     VSATEARERATLAQDEFEQVQDQAGGHDEDRSELAAAHERATAMLAECTERAKVLRTA
                     ERESARDLAARRARADALAEAARRGADASAALLADPARFGGVVGSFAARLRVAEGYEV
                     AIAAALGAAAEAVAVAGLDAAAEILATLRRDDAGGASLVIAGEAATPTPLIADEATPA
                     PPTSSAPPTPSATPAPTTPSAPHGSGPIPANELVRAPDGLAEAAAELLCDVVVTADLE
                     QARRYVRDNPGFKAVTRDGDLLGAYWAQGGGARPQSLFEMRAAADEAAAGLAEAELRC
                     DEAARQLAVAAEAEEDARQAVARTQARRHEVDAAAAEVSGRLGRLAGAARAAREEAAR
                     LDSAITSARQAAEKDLARLEQLRAELAEAEELDATDEPDEPQHQTAREDLAEFCTAAR
                     NAEMEARLEVRTAEERLRAIGGRAESLAAAAAAERATRQRAAERRQRRATQAAVARAV
                     ALGARYAVEAAEQSVNQALARRTEAEAANSGGAAELKAVRTRVRDLAADLDTVVNTAH
                     GTEIARAEHRLRLQQLAEHALEEFGVEADALVAEYGPEVPVPVIGDDAAVPSRFERTA
                     AERRAADAQRQLDRLGKVNPLALEEFEALTERHTFLATQLEDLRKTRRDLLTVVKEVD
                     DRVQEVFASAYADTAREFEHLFSRLFPGGQGNLVLTEPDDMLATGIEIEARPPGKKVK
                     RLSLLSGGERSLTAIAYLFAIFKARPSPFYVLDEVEAALDDTNLQRLLLVFEELRESS
                     QLIV"
BASE COUNT          371 a          979 c          972 g          258 t
ORIGIN      
        1 ctgaccgagg cgaccgagcc ggaacgcccc ggtcgcgacc ccgacgagct cgaccgccag
       61 gccgacgaac tgcgggaaga agaggagacc ctgaacgagc ggctgacggc cgggcgcgag
      121 gtgctcgccg tcgccaccgc cgggcgcacc gccgccgagc gggagctcgc cgcggccgaa
      181 cgccgccagg ccgagggggc caaggccgcc gccgcccggg ccggccgggt cgcccggctg
      241 cgcgagcagg tcggcgcggc ggacagccgc accgtggccg cgcaggagga aatggaccgg
      301 ctggaggtct ccgcgaccga ggcccgggag cgcgccaccc tggcccagga cgagttcgag
      361 caggtgcagg accaggcggg cgggcacgac gaagaccgta gcgagctggc cgccgcgcac
      421 gagcgggcca ccgccatgct cgccgaatgt accgaacggg ccaaggttct ccgcaccgcc
      481 gaacgtgaat cagctcgtga cctggccgcc cggcgggcgc gcgccgatgc gctggccgag
      541 gccgcccggc gcggcgcgga cgcctccgcg gcgctgctcg ccgacccggc caggttcggc
      601 ggcgtggtcg gctcgttcgc cgcgcggctg cgggtagccg aggggtacga ggtggccatc
      661 gccgcggccc tgggtgccgc cgccgaggcg gtggcggtgg ccggcctgga cgcggccgcc
      721 gagatcctgg ccaccctgcg ccgcgacgac gccggcggcg cctccctcgt catcgccggc
      781 gaagccgcca cccccacccc gctcatcgcc gacgaagcca cccccgcccc accaacctca
      841 tccgccccac caaccccatc cgccacacca gcacccacaa ccccatcagc cccacacggc
      901 tcagggccca tcccggctaa cgagctggtg cgcgcgcccg acgggctggc ggaggccgcg
      961 gccgagcttc tgtgcgacgt agtggtgaca gcggacctgg agcaagctcg ccggtacgtt
     1021 cgggacaatc ctggttttaa ggccgtgacc agggacggtg acctgctcgg ggcctattgg
     1081 gcgcagggcg gcggcgcccg gccgcagagc ctgttcgaga tgcgggccgc cgccgacgag
     1141 gcggccgcgg ggctggccga ggccgaactg cgctgcgatg aggcggcccg ccagctggcg
     1201 gtggcggccg aggccgagga ggacgcccgg caggcggtgg cgcggacgca ggcccgcagg
     1261 cacgaggtcg acgcggccgc ggcggaggtc tccggccgtc ttggccggct ggccggggcg
     1321 gcccgcgccg cgcgggagga ggccgcgcgg ctcgatagcg ccatcacctc ggcccgccag
     1381 gcggccgaga aggacctggc ccggctggag cagctccgcg ccgagctcgc cgaggccgaa
     1441 gagctagacg ccaccgacga gccggacgag ccgcagcatc agacggcccg cgaggacctc
     1501 gccgagttct gtaccgcggc gcgcaacgcc gagatggagg ccaggctcga ggtcaggacc
     1561 gcggaggagc ggctgcgagc gatcggcggc cgcgccgagt ccctcgctgc cgcggcggcg
     1621 gccgagcgcg ccacccgcca gcgcgcggcc gagcgccgtc agcgccgggc cacccaggcc
     1681 gccgtggccc gggccgtcgc gctgggcgcg cggtacgcgg tcgaggcggc cgagcagtcg
     1741 gtaaaccagg ccctggcccg ccggaccgag gccgaggcgg ccaacagcgg cggcgccgcc
     1801 gagctgaagg cggtccgcac ccgggtccgc gacctggccg ccgacctgga caccgtggtg
     1861 aacaccgcgc acggcaccga gatcgcccgg gccgagcacc ggctgcggct gcagcagctg
     1921 gccgagcacg cgctggagga gttcggcgtc gaggcggacg ccctggtggc cgagtacggc
     1981 cccgaggtgc ccgtgccggt gatcggagac gacgccgccg tgccgtcgag gttcgagcgc
     2041 accgccgccg agcgccgcgc cgccgacgcg cagcgccagc tggaccggct gggcaaggtc
     2101 aacccgctgg ccctggagga gttcgaggcg ctcaccgagc ggcacacctt cctggctact
     2161 cagctcgagg acctccgcaa gacccggcgt gacctgctca ccgtggtcaa ggaggtcgac
     2221 gaccgggtgc aggaggtgtt cgcctccgcc tacgccgaca ccgcccgcga gttcgagcac
     2281 ctgttcagcc gcctgttccc cggcggccag ggcaacctgg tgctgaccga acccgacgac
     2341 atgctcgcca ccggcatcga gatcgaggcg cgcccgccgg gcaagaaggt caagcggctg
     2401 tcgctgctgt ccggcgggga gcggtcgctg accgccatcg cctacctctt cgcgatcttc
     2461 aaagcccgcc cctcgccgtt ctacgtgctc gacgaggtgg aagcggccct ggacgacacc
     2521 aacctgcagc ggctgctcct ggttttcgag gagctcaggg agagttccca gctcatcgtc
//
LOCUS       DASPYY010000074         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_714518, whole genome shotgun sequence.
ACCESSION   DASPYY010000074 DASPYY010000000
VERSION     DASPYY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714518"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="phaZ"
                     /locus_tag="VEP73_01010"
     CDS             <1..695
                     /gene="phaZ"
                     /locus_tag="VEP73_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HYT25052.1"
                     /translation="DALDPSVEVIRFDVPGVGGSLLPRLPYRLPTLARLVAGLLDQLG
                     YTSVDVLGVSWGGGLAQQFALCHRDRCRRLVLVATSTGMLMVPGRPSALARMATPRRY
                     LDPDYAERIAADLYGGTIRSDPGRARALLHGRARVGPRLGYLYQLAAGAGWSSLPFLP
                     WIRQPTLLLAGDDDPIVPLVNARIMRRLLPDAELHVYHGGHLGLLTEAAELVPVVDRF
                     LCGDGRSADVPT"
     gene            748..1920
                     /locus_tag="VEP73_01015"
     CDS             748..1920
                     /locus_tag="VEP73_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT25053.1"
                     /translation="MPADADFYALELLLDDEEDRALLHRVRAFMEKEVQPVINQYWTR
                     AKFPHELLPGLRELGVAGLPYSGYGCPGRSPLLDGMVAMELARTDPSIATFNGVHSGL
                     AMGSIYLCGSEEQKQRWLPEMARLEKIGAFGLTEPEVGSGASRGLATTARRDGDAWVL
                     DGRKKWIGNASFADLTVIWARDADDGQVKGFVVERGTPGFAAEDLKDKIALRVVQNAL
                     VTLDGVRVPEANRLQHAGSFRDTAEVLRMTRAGVAWMAVGCARGAYEHALAYAVEREQ
                     FGRPIGGFQLVQDLLVRMLGNITASATLCARLSQLQAAGLARDEHASLAKAFCTVRMR
                     ETVGYARELLGGNGILLEHHVGRFVADAEAIYSYEGTREMNTLIVGRAITGLSAFV"
     gene            complement(1943..>3097)
                     /locus_tag="VEP73_01020"
     CDS             complement(1943..>3097)
                     /locus_tag="VEP73_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25054.1"
                     /translation="LMSDRRDAWERWYGDVAAARWGTAVWHTLAWAVFGGAYVGAVVL
                     VSSGLRATPGDVLLVLAAGSRLSSYIGATVGEIGFLRGIWMDGSRRVAWLEDYAAALV
                     ASADQAVPAALTRGIRVEHVSFAYPGTDRLVLDDVCLDLPTGSVVAIVGENGAGKTTL
                     VKLLCKLYEPTSGRILVDGLPLARMPADEWRSRLAGAFQDFFRFELRARHSVGVGDVP
                     RLDDEPAVVGAVHRAGADDVVARLAAGLETQLGPTWPDGVDVSFGQWQKLALARGFMR
                     DRPLLLVLDEPTAALDAETEHALFERYAAAARGDGQAGGDSAGGGSAGRAPDGRVTIL
                     ISHRFSTVRMADLIVVLDGARVLEVGSHQELMARGGQYAELYGIQAAAYR"
BASE COUNT          436 a         1162 c         1109 g          390 t
ORIGIN      
        1 tcgacgccct cgacccgtcg gtcgaggtga tccgcttcga cgtccccggc gtcggcggct
       61 ccctcctgcc aaggctcccc taccgcttgc cgacgctggc gcgtctggtc gccggcctcc
      121 tcgaccagct cggctacacg agcgtcgacg tgctgggcgt ctcctggggc ggcggcctgg
      181 cgcagcagtt cgcgctgtgc caccgcgacc gctgccggcg gctggtcctg gtcgccacca
      241 gcaccggcat gctgatggtg ccgggccggc cgagtgccct cgcgcggatg gccacacccc
      301 gccggtacct ggacccggac tacgccgagc ggatcgccgc cgacctctac ggcgggacca
      361 tccggtccga cccgggcagg gcgcgggcgc tcctgcacgg ccgggcccgc gtgggccccc
      421 ggctcggcta cctctaccag ctcgccgccg gcgcgggctg gagcagcctg ccgttcctcc
      481 cctggatccg gcagccgacg ctgctgctcg ccggcgacga cgaccccatc gtcccgctcg
      541 tcaacgcccg catcatgcgg cgcctcctgc ccgacgccga gctgcacgtc taccacggcg
      601 gccacctcgg actgctcacc gaggcggccg agctcgtccc cgtggtggac cggttcctgt
      661 gcggcgacgg ccggagcgcc gacgtcccca cctgaaccgt gccagccaga ccgtgccagc
      721 cagaccgtgc ccgccaagga ggtcccgatg cccgccgacg ccgacttcta cgccctggag
      781 ctgctgctcg acgacgagga ggaccgcgcg ctgctccacc gcgtgcgggc gttcatggag
      841 aaggaggtgc agccggtcat caaccagtac tggacccggg cgaagttccc ccacgagctc
      901 ctgcccggcc tgcgcgagct cggcgtcgcc gggctcccgt acagcggcta cggctgcccg
      961 ggcaggagcc cgctgctcga cggcatggtc gcgatggagc tggcccgcac cgacccgtcg
     1021 atcgcgacct tcaacggcgt ccacagcggg ctggcgatgg gctcgatcta cctgtgcggc
     1081 tcggaggagc agaagcagcg ctggctgccg gagatggccc gcctggagaa gatcggcgcg
     1141 ttcgggctga ccgagcccga ggtcggctcg ggcgcgtcgc gaggtctcgc caccacggcc
     1201 cgccgcgacg gcgacgcctg ggtgctcgac gggcgcaaga agtggatcgg caacgcgagc
     1261 ttcgccgacc tgaccgtgat ctgggcaaga gacgccgacg acgggcaggt caagggcttc
     1321 gtcgtcgagc gtgggacgcc cgggttcgcc gccgaggacc tcaaggacaa gatcgcgctg
     1381 cgggtggtgc agaacgccct cgtcaccctg gacggcgtcc gggtccccga ggccaaccgg
     1441 ctgcagcacg ccggctcgtt ccgggacacc gccgaggtgc tgcggatgac ccgggccggg
     1501 gtggcctgga tggcggtcgg ctgcgcgcgc ggcgcgtacg agcacgcgct cgcctacgcc
     1561 gtcgagcgcg agcagttcgg ccggcccatc ggcggcttcc agctggtcca ggacctgctc
     1621 gtccggatgc tggggaacat caccgcatcg gccacgctgt gcgcccggct gtcgcagctg
     1681 caggcggccg gcctggccag agacgagcac gcgtcgctgg cgaaggcgtt ctgcaccgtg
     1741 cggatgcgcg agacggtcgg ctacgcccgc gagctgctgg gcggcaacgg gatcctcctc
     1801 gagcaccacg tcggccggtt cgtcgccgac gccgaggcga tctactccta tgaaggcacc
     1861 cgcgagatga acacgctgat cgtcggccgc gcgatcaccg gcctcagcgc cttcgtgtga
     1921 gcgcgctccc acccgccgct cgctaccggt aggccgcggc ctggatgccg tacagctcgg
     1981 cgtactggcc gcccctggcc atcagctcct ggtggctgcc gacctcgagg acgcgggcgc
     2041 cgtcgagcac gacgatcagg tcggccatgc gcacggtgga gaaccggtgc gagatgagga
     2101 tggtgacgcg gccgtcgggg gcgcggccgg cggagccacc gcccgcgctg tcaccgcccg
     2161 cctggccgtc gccgcgggcc gcggcagcgt agcgctcgaa cagggcgtgc tcggtctcgg
     2221 cgtccagtgc ggcggtgggc tcgtcgagga ccagcagcaa cgggcggtcg cgcatgaagc
     2281 cacgcgccaa ggcgagcttc tgccactggc cgaaggagac gtcgaccccg tccggccacg
     2341 tcggaccgag ctgggtctcg agcccggcgg ccaggcgcgc gaccacgtcg tcggccccgg
     2401 cgcggtggac ggcgccgacc acggccggct cgtcgtcgag gcggggcacg tcgccgacac
     2461 cgacgctgtg gcgggctcgg agctcgaagc ggaagaagtc ctgaaacgca cctgccagcc
     2521 gtgaccgcca ctcgtcggca ggcatgcggg ccagcggcag gccgtccacc aggatccggc
     2581 ccgaggtcgg ctcatagagc ttgcacagca gtttcaccag cgtcgtcttg ccggcgccgt
     2641 tctcgccgac gatggcgacc acggagccgg tgggcaggtc gaggcagacg tcgtcgagca
     2701 ccagccggtc ggtgcccgga taggcgaacg acacgtgctc gacgcggatg ccccgggtca
     2761 gcgccgccgg caccgcctgg tcggccgagg cgacgagcgc cgcggcgtag tcctcgagcc
     2821 aggcgacgcg gcgcgacccg tccatccaga tgccacgcag gaacccgatc tcgccgaccg
     2881 tggcaccgat gtaggacgac aaccgggagc cggccgccag caccagcagc acgtcgccag
     2941 gggtggcacg gaggccggac gacacgagca ccacggcgcc gacgtaggcc ccgccgaaca
     3001 ccgcccacgc gagcgtgtgc cagaccgcgg taccccaacg ggccgcggcc acgtcgccat
     3061 accaccgttc ccatgcgtcc cggcggtcgg acatcag
//
LOCUS       DASPYY010000075         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_293181, whole genome shotgun sequence.
ACCESSION   DASPYY010000075 DASPYY010000000
VERSION     DASPYY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293181"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VEP73_01025"
     CDS             complement(<1..609)
                     /locus_tag="VEP73_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYT25055.1"
                     /translation="MGTAPPPGDLTALNASFARTLRAENKSERTVQAYAEAVRLLARF
                     CDANGLPITASAIRRAHLERFVSDQLARWKPATAHARYRGLHAFFVWAVAEGELDASP
                     MAGMRPPRVPETPVPVLTEDQLRRLLKVCEGRGFLARRDTALIRVFADTGARRAEVAG
                     MRLDDLHLDDQVLLVVGKGRRPRGLAFGRRTALVLDRYLRVRA"
     gene            complement(738..1400)
                     /locus_tag="VEP73_01030"
     CDS             complement(738..1400)
                     /locus_tag="VEP73_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT25056.1"
                     /translation="MRSLMDWFLLLGLRFVFVLYMVLVLGYSVFQRLYAYRSARRSRH
                     RRAAGAPPGSYRPTVDVVVPCFNEEPELLAACCASLDRQDYEGELRVWVVDDGSRRNR
                     EQLLGVLERYAVAEEVHLDPETTEVRVTEPAHRPGGRAGPATRHRWTVLLLTGNAGKR
                     LAQHAAVHHGDGDLVLTVDSDTMLAPDGVRSIVAAFRDGRVGAVTGNIAVENADVNLL
                     TR"
     gene            1727..>2351
                     /locus_tag="VEP73_01035"
     CDS             1727..>2351
                     /locus_tag="VEP73_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT25057.1"
                     /translation="MTRCRCGAENPDNANFCLACGRRLAAVPAADRDVLRTVTVVFTD
                     VVDSVGLASRLGTETWRSVVSRYFEKARAVLEQYGGTVQKFAGDAVMAVFGVEELHED
                     DAVRAVSAAFELHRALPGLNDELEREWKVRLALHTGVNTGEVTVDYPVEGHMLVLGEA
                     INLAARLQSAAGPDEILIGHPTYRLVRDSVAVDPVEALRLRGIETAVT"
BASE COUNT          350 a          806 c          883 g          312 t
ORIGIN      
        1 cgcccgcacc cgcaggtagc ggtcaaggac cagcgcggtc ctgcggccga aggccaggcc
       61 gcgaggccgc cggcccttgc cgaccacgag caggacctgg tcgtcaaggt gcaggtcgtc
      121 caggcgcatg cccgcgacct cggcgcggcg cgcaccggtg tcggcgaaca cgcggatcag
      181 ggcggtgtcg cgccgagcca ggaagccgcg gccctcgcag accttcagca gccggcggag
      241 ttgatcttcg gtgaggaccg ggacgggcgt ctcgggcacc cggggcgggc gcatcccggc
      301 catggggctc gcgtccagct caccctcggc aacggcccag acgaagaacg cgtgcagccc
      361 gcggtagcgg gcgtgggcgg tcgccggctt ccagcgggcc agctggtcgc tgacgaaccg
      421 ctcgaggtgg gcgcgccgga tcgcgctggc ggtgatcggc aagccgttgg cgtcgcagaa
      481 ccgggccagg agccggacgg cctcggcgta ggcctggacg gtccgctcgc tcttgttctc
      541 cgcgcgcagt gtgcgggcga agcttgcgtt cagggcggtg agatcgcccg gcgggggagc
      601 ggtacccatg gaccacaagt taggtcaact tcgagcgcta tgcaagcggc aaccgctcgc
      661 gtggagccga cgcacatagc ctctgagctg cacagatagc gacgatgggc ccgccgacgg
      721 ggcgctcact agccgaccta cctggtcagc aggttgacgt cggcgttctc caccgcgatg
      781 ttcccggtga cggccccgac tcgcccgtcg cggaaggcgg cgacgatcga ccggaccccg
      841 tcgggcgcca gcatggtgtc ggagtcgacc gtgagcacga ggtcgccgtc gccgtggtgg
      901 acggccgcat gctgcgccag gcgcttgccg gcgttaccgg tcagcagcag gaccgtccac
      961 cggtggcgcg tggccgggcc ggcgcggccg cctggccggt gcgcgggctc ggtcacccgc
     1021 acctcggtcg tctcggggtc gaggtggacc tcctcggcga ccgcgtagcg ctcgagcacg
     1081 ccgaggagct gctcccggtt cctccgcgac ccgtcgtcga cgacccagac gcgcagctcc
     1141 ccctcgtagt cctgtcggtc gagcgacgcg cagcaggcgg cgagcagctc gggctcctcg
     1201 ttgaagcacg ggacgacgac gtcgacggtg ggtcggtagg agccgggcgg cgcgcccgcc
     1261 gcccgccgat gccggctgcg cctcgcgctc ctgtacgcgt agagccgctg gaagaccgag
     1321 tagccgagca ccagcaccat gtagagcacg aacacgaagc gcaggccgag gagcaggaac
     1381 cagtccatga ggctacgcac gggctcgcgc ggcgagcgcc atggcagcaa cggagggaac
     1441 cggggcaaag ggggtgctgg cgatgtccgg tatcgtcatg ctctgcttgc tcggcggaac
     1501 gtctacggca gtagcattat aggtggctag gcttgcccgt ccaggatgaa agagatgctg
     1561 gaattgtctt gatgatccgt cgggcggagg cctggggcgc cgtcgaggag cgccggcggc
     1621 ccggtcgggc gtgcgcacga cagctcggct gacgcctccg cgacagtggc cggggcgacc
     1681 aggtgcctag catcggggac gagccgagat cgagaggtcc caagggatga cacggtgccg
     1741 ctgcggcgcg gagaacccgg acaacgccaa cttctgcctg gcgtgcggac gccggctcgc
     1801 cgccgtcccg gccgcggacc gcgacgtgct ccggaccgtg acggtcgtct tcaccgacgt
     1861 cgtggactcg gtcgggctgg ccagccgcct cggcaccgag acctggcggt ccgtcgtgag
     1921 ccggtacttc gagaaggcac gcgcggtgct cgagcagtac ggcggcaccg tgcagaagtt
     1981 cgccggcgac gcggtcatgg cggtgttcgg cgtcgaggag ctgcacgagg acgacgccgt
     2041 ccgggcggtg agcgccgcct tcgagctcca ccgcgcgctt cccggcctga acgacgagct
     2101 cgagcgggag tggaaggtgc gcctcgcgct gcacaccggc gtgaacaccg gcgaggtgac
     2161 ggtcgactac cccgtcgaag ggcacatgct cgtcctcggc gaggccatca acctcgccgc
     2221 gcgcctgcag tcggccgccg gccccgacga gatcctcatc ggccacccga cctaccggct
     2281 cgtgcgcgac tcggtcgccg tcgacccggt cgaggcgctc aggctccggg ggatcgagac
     2341 ggcggtgacg g
//
LOCUS       DASPYY010000076         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_12725, whole genome shotgun sequence.
ACCESSION   DASPYY010000076 DASPYY010000000
VERSION     DASPYY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12725"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEP73_01040"
     CDS             complement(<1..431)
                     /locus_tag="VEP73_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT25058.1"
                     /translation="MIRTSGLTKRYGQLVAVDGLDLDVHEGDLFGFLGPNGSGKTTTV
                     RMLLGLVFPTSGTVELLGRPMPRAQRSVLPQVGSLIEGPAFYPHLPGRTNLALFDAAG
                     PAPAQRAGPGGRRADRRRRIGDALARVGLDGVGRRPLKAYS"
     gene            complement(440..1549)
                     /locus_tag="VEP73_01045"
     CDS             complement(440..1549)
                     /locus_tag="VEP73_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HYT25059.1"
                     /translation="MRFLPRSRWSWRWLALLAGVAVLASVPAIVAAMPAPGREVAAAD
                     LLGRVRASGPVAYQGYAESRAGLGLPDVPRVGRVVALLGETTRMRAWVRSPTAWRVDE
                     LTAIGERDTYQDARGTWEWDSGERRASRTGGEAAVRLARPADLLPPELGRRLAAAAEP
                     GEARRLGSRRVAGVTAAGLRIVPRSPDTTVGRVDLWADPRTGLPLRVEVTARGAHAPI
                     LTTAFLDLRQTAPGNEAVTFHLPLDAEVENDEAPDLARAIDRFSPFVLPSDLAGLPRR
                     TLVGRAAGTFGQGFEVVAALAFPAGYSPRTQAVLDAVPQVKGPWGEAGLVTTPLLNAL
                     VFDRSGVAYALAGTVPPRVLERVATQLARNGAAPA"
     gene            1679..2524
                     /locus_tag="VEP73_01050"
     CDS             1679..2524
                     /locus_tag="VEP73_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HYT25060.1"
                     /translation="MAYHVELKPGEKVELADHDPDADGGLDKQAGKKALVPLLEELDA
                     LQQELYAAGVNAVLAVLQGMDTSGKDGTIRKVFAGVNPQGCQVTSFKVPTPEEAAHDF
                     LWRVHRVAPAKGMLGVFNRSHYEDVLAVRVHELVPPKVWKARYDQINDFERTLALNGT
                     IVCKFFLHVSQDEQERRLLAREADVEKAYKLSPGDWRERRYWDDYKAAYQDALGRCAT
                     REAPWHLVPANRKWYRNLAVAEALVERLRGYRKDWQRELERMSRERLAELAKVKAEEA
                     EEAAG"
BASE COUNT          369 a         1084 c         1170 g          326 t
ORIGIN      
        1 ctcgagtacg ccttgagggg ccggcgcccg accccgtcga ggccgacccg ggccagcgcg
       61 tcgccgatgc gccggcgccg gtcggcgcgg cgccccccgg ggcccgcgcg ctgcgcggga
      121 gcgggcccgg cggcgtcgaa cagcgccagg ttcgtgcgcc cgggcaggtg ggggtagaag
      181 gcggggccct cgatcagcga gccgacctgg ggcaggaccg accgctgcgc gcggggcatc
      241 ggccggccga gcagctcgac cgtgcccgag gtcgggaaca cgagcccgag cagcatccgg
      301 acggtggtcg tcttgcccga cccgttcggg ccgaggaacc cgaacaggtc cccctcgtgg
      361 acgtcgaggt cgaggccgtc gacggcgacg agctgcccgt agcgcttggt cagcccgctg
      421 gtgcggatca cggcggcgct cacgccggcg ccgccccgtt ccgggcgagc tgggtggcga
      481 cccgctcgag gacccgcggc ggcaccgtgc cggcgagggc gtaggcgacc cccgagcggt
      541 cgaagacgag cgcgttcagc agcggggtgg tgaccaggcc ggcctcgccc cacgggccct
      601 tcacctgcgg caccgcgtcc agcaccgcct gcgtgcgggg cgagtacccg gccgggaagg
      661 ccagggcggc gaccacctcg aacccctggc cgaaggtgcc cgccgccctg ccgaccaggg
      721 tgcggcgggg cagcccggcg aggtccgagg ggagcacgaa cggcgagaag cggtcgatgg
      781 cgcgggcgag gtcgggcgcc tcgtcgttct cgacctcggc gtcgagcggg aggtggaagg
      841 tcacggcctc gttgccgggg gcggtctggc gcaggtcgag gaaggcggtg gtgaggatcg
      901 gggcgtgcgc gcctctggcg gtcacctcga cccgcagggg caggcccgtc cgcgggtcgg
      961 cccacaggtc gacccggccc accgtggtgt ccggcgagcg cgggacgatg cgcaggccgg
     1021 cggcggtgac gccggcgacg cggcggctgc cgagccggcg cgcctctccg ggctcggcgg
     1081 cggcggcgag ccggcgtccg agctctggcg ggagcaggtc ggccgggcgg gccaggcgca
     1141 ccgcggcctc ccccccggtc cggctggcgc gccgctcgcc cgagtcccac tcccaggtgc
     1201 cccgcgcgtc ctggtaggtg tcgcgctcgc cgatggcggt cagctcgtcg acccgccagg
     1261 cggtcgggct ccgcacccag gcgcgcatgc gggtggtctc gccgagcagg gcgaccaccc
     1321 ggccgaccct gggcacgtcg ggcagcccga gcccggcccg gctctcggcg tagccctggt
     1381 aggcgaccgg gccggacgcc cggacccggc cgagcaggtc ggcggcggcg acctcgcggc
     1441 cgggggcggg catggccgcg acgatcgcgg ggaccgaggc aagcaccgcg acgccggcca
     1501 gcagcgcgag ccagcgccac gaccatcggg aacgtggcag gaaccgcatc gtttctccac
     1561 ggtacgacgg tcccgccgat cgcgggcggc cacggggcca gggccgctcc gccgcccccc
     1621 ttcggctacg ctcccgtcgg acggcccgga gcaggccggg ccggggagag gaggccgcat
     1681 ggcgtaccac gtcgagctga agccgggcga gaaggtcgaa ctcgccgacc acgacccaga
     1741 cgcggatggc ggcctcgaca agcaggccgg caagaaggcg ctcgtccccc tgctcgagga
     1801 gctggacgcg ctgcagcagg agctgtacgc ggccggcgtc aacgccgtcc tcgcggtcct
     1861 gcagggcatg gacaccagcg gcaaggacgg caccatccgc aaggtgttcg cgggcgtgaa
     1921 cccgcagggc tgccaggtca cctcgttcaa ggtgccgacc ccggaggagg cggcccacga
     1981 cttcctgtgg cgggtgcacc gggtcgcgcc cgccaagggc atgctcgggg tgttcaaccg
     2041 ctcccactac gaggacgtgc tggccgtccg cgtccatgag ctggtcccgc cgaaggtctg
     2101 gaaggcccgc tacgaccaga tcaatgactt cgagcgaacc ctggcactga acggcaccat
     2161 cgtgtgcaag ttcttcctcc acgtctccca ggacgagcag gagcgccggc tgctcgcccg
     2221 cgaggccgac gtcgagaagg cgtacaagct ctcccccggc gactggcgcg agcgccgcta
     2281 ctgggacgac tacaaggcgg cctaccagga cgccctcggc cgctgcgcca ccagggaggc
     2341 gccctggcac ctcgtccccg ccaaccggaa gtggtaccgc aacctcgccg tcgccgaggc
     2401 cctggtcgag cgcctgcggg ggtatcgcaa ggactggcag cgggagctcg agcggatgag
     2461 cagggagcgc ctggccgagc tggccaaggt gaaggcggag gaggcggagg aggcggccgg
     2521 ctgagcgcgg ggggtcgtgg gtccgtctcg gacgggccgg gcggctggcc gccctggcgg
     2581 cggtcacggc ggctctgctc gcggccggct ggcggctgcg ctccggccag gcccgggcca
     2641 tgcggcgcct cgccagcgtg ctgtggctgc tctcgacggg agcctgggcc gggctctgcg
     2701 ccgtgctcgc cggcggccgg gccgacgccc tcgccaccgg cccgctgccc gtcgccgcgg
     2761 gcacgctcgc ctatgccgcc gtgcctgtgg cgcttcgagc ggcgcgccct ccagcaggtc
     2821 gcgctgttcg cggcggccat gactaccgtg gcggcgacgg ccgcccaggt cgggcggtcg
     2881 gccggggtca cgtccggcat cggcttcctg gcgctggggg cggcctggct cgagctcggc
     2941 cggcgcggc
//
LOCUS       DASPYY010000077         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1136946, whole genome shotgun sequence.
ACCESSION   DASPYY010000077 DASPYY010000000
VERSION     DASPYY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136946"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..388)
                     /locus_tag="VEP73_01055"
     CDS             complement(26..388)
                     /locus_tag="VEP73_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYT25061.1"
                     /translation="MARLLFHCTHGADDPERAIVPFIAANVAAVSGQDAVVLLTAEGA
                     WLCKRGYADGVRLDGYPELGALVADFVAAGGAIWGCSACTTPRGITADDLVPGAAIVG
                     AAAIVEAVAGGAIPVALA"
     gene            complement(401..889)
                     /locus_tag="VEP73_01060"
     CDS             complement(401..889)
                     /locus_tag="VEP73_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HYT25062.1"
                     /translation="MRYVLKQSLLARASDYAISDESGRTAFVVHGRAFGIGRKLSLKD
                     AAGAEVASVRERRLALRPRYEVSRAGRVAAVVRTVPFTSRRRFVVQAPGQHGFRVTGN
                     FFANDYLLSRGDRRVARVYKELFTMTDTYAVDVADGEDPVVVLATAVVIDLVCHQPGS
                     RG"
     gene            1006..>1932
                     /locus_tag="VEP73_01065"
     CDS             1006..>1932
                     /locus_tag="VEP73_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT25063.1"
                     /translation="MGRTRMVVIGGDAAGMSAASQARRRRPASDLEIVAFERGGHTSY
                     SACGLPYHAGGMVDDWHDLVTRTPAQFAGQDIDARTRHEVESIDLEAGRVRVRDLDAG
                     RAFDEPWDVLVVTTGSEPARQPIPGIDLPGVHALSILQDGIDLRRAVDAKPARAVVIG
                     GGYIGLEAAEALVYRGIPTSLVERGPEVMNRIDPDMGALVGQALREAGVDVFTGEKVE
                     AIEGDDRVRAVRTATRTLPADLVVLGLGTRPRTGLAREAGITLGQTGAIVVGPRMQTP
                     HEGVWAAGDCAETRHLVSGAPVNYHLGTIANKQ"
BASE COUNT          271 a          714 c          724 g          223 t
ORIGIN      
        1 ggcgcttcga tcgagcgggc ctcgctcagg cgagcgcgac cgggatggcc ccgccggcca
       61 ccgcctcgac gatggcggcg gcaccgacga tggccgcccc ggggacgagg tcgtcggcgg
      121 tgatgccgcg cggggtcgtg caggcgctgc acccccagat cgcgccaccg gcggccacga
      181 agtcggccac gagcgcgccg agctccgggt agccgtcgag gcggacgccg tccgcgtacc
      241 cccgcttgca gagccacgcc ccctcggcgg tgagcagcac gaccgcgtcc tgaccggaca
      301 cggcggccac gttggcggcg atgaacggga cgatggcgcg ctcgggatcg tcggcgccgt
      361 gggtgcagtg gaagagcagc ctggccatgc gtggctcctt tcagccgcgg ctccccggct
      421 ggtggcagac gaggtcgatg accacggcgg tcgcgagcac gacgaccgga tcctcgccgt
      481 cggcgacgtc gacggcgtac gtgtcggtca tcgtgaacag ctccttgtag acccgggcga
      541 cccgccggtc gccgcgggag agcaggtagt cgttcgcgaa gaagttgccg gtgacccgga
      601 agccgtgctg gcccggcgcc tggacgacga accgccgccg ggaggtgaac ggcaccgtcc
      661 tcaccaccgc cgccacccgg ccggcgcggg acacctcgta tctcgggcgc agcgccagcc
      721 tccgctcccg cacggacgcg acctcggcgc cggccgcgtc cttgagcgac agcttccggc
      781 cgatcccgaa cgcgcggccg tggacgacga aggcggtccg gcccgactcg tcggagatgg
      841 cgtagtcgct cgcccgggcg agcagcgact gcttcagcac gtagcgcacc gcgagatcct
      901 acccggggcg aagcgcccgc cgtcgccctc ggggcgaagc gcccgccgtc gcccggatcg
      961 ggcagactag gcgggagtgg tcgagcctct cccaaggagc gggtcatggg gcgcacgcgg
     1021 atggtcgtga tcggcggcga cgccgccggg atgagcgcgg cctcgcaggc tcgcaggcgg
     1081 cggcccgcct ccgacctcga gatcgtcgcc ttcgagcggg gcgggcacac ctcgtactcc
     1141 gcctgcggcc tgccatacca cgccggaggg atggtcgacg actggcacga tctggtcacc
     1201 cgcacgcccg cccagttcgc cgggcaggac atcgacgccc gcacgcgcca cgaggtcgag
     1261 agcatcgacc tcgaggccgg gcgggtgcgg gtccgcgacc tggacgccgg gcgggcattc
     1321 gacgagccct gggacgtgct cgtggtcacc accgggtcgg agccggcacg gcaaccgatc
     1381 cccggcatcg acctgcccgg cgtccacgcc ctgtcgatcc tgcaggacgg catcgacctg
     1441 cgccgggcgg tcgacgcgaa gccggcgcgg gccgtcgtga tcggcggcgg ctacatcggc
     1501 ctcgaggcgg ccgaggcgct ggtctaccgc ggcatcccga cgtcgctggt ggagcgcggc
     1561 cccgaggtga tgaaccggat cgacccggac atgggcgcgc tggtcggcca ggcgctcagg
     1621 gaggccggcg tcgacgtctt caccggcgag aaggtcgagg cgatcgaggg cgacgaccgg
     1681 gtgcgcgcgg tgcgcacggc cacgcggacc ctgcccgccg acctcgtcgt cctcggcctc
     1741 ggcacgcggc cgcggacggg tctcgccagg gaggccggca tcacgctcgg ccagaccggc
     1801 gcgatcgtgg tcgggccgcg catgcaaacg ccccacgagg gcgtctgggc ggccggcgac
     1861 tgcgccgaga cgaggcacct cgtctccggc gccccggtca actaccacct cggcacgatc
     1921 gccaacaagc ag
//
LOCUS       DASPYY010000078         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2540776, whole genome shotgun sequence.
ACCESSION   DASPYY010000078 DASPYY010000000
VERSION     DASPYY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540776"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3148
                     /locus_tag="VEP73_01070"
     CDS             <1..>3148
                     /locus_tag="VEP73_01070"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HYT25064.1"
                     /translation="LALAALRGLGAGPWLAVRLWRALLLLVAGWGAARLFADVTRDET
                     AADAAGRGRAGAAGRVAAAVFYVANPYVVVAGATDPILVPYAFLPWLVLALARSVRRP
                     RSWRAPAAFALGFALCGGMNAGVVPLFMLLAVPCYLAYARRALGVGWRDLGAAAGRCL
                     GLALLVSLYWLVPAALASSSGAAIAATTETPRDVAGPSSYAETLRLLGLWTLYGQAGG
                     RPFLPGMVAYLTNPLVVLASFALPVAATASALASRARARLLPAALLAAAVPVMVGLFP
                     TAGPSPFGRVLEAAFRRLPGAIAFRTTNKVGALVALALTLLLALGAAELAGWAEARRS
                     SGPLSPQRSAGWAGPRWLGAAGRPPSGRLAGGIGAAMAAVLALAVLPAWTGGLNLARY
                     RVPGYWRQAAADLNRGPASSRVLVLPGQVQADYRWGLEGPDDLDASLLTRPSVVRSTV
                     PNGSKEQANFLAALDLALPPTSPDTAGVGTMARYLGAGEVLARYDRVWEAAGGTPPSV
                     LAAALRQDPGLRREAAYGRPGEHTVAPHGGAPGDAGLAPLERFAVAGARPVQRTEPAQ
                     GTLLIDGDSFALPPLDRLGRLQGQPPFRLLGSTSLDEAALALDDGARIVLTDTNRRRE
                     WDPHRSGPSFSPTLRADQPIADGASSLTLSGDPLSGDPSSGDPADQSVAVLDGARAVT
                     ATSSGPAFGLAPWGKPSFAFDGDPRTAWVTGAYGTAVGQSITIEFGRRVRISRLTLRP
                     LLGSPAQVSSVRISLDGRSWDAEVPARPEVTVPVPETDAYAATVEITGVRGGAGLNPV
                     GFYEIGIPGVRATEGVRMPERFRELAGSLDTGAGKRLAATPIEVVMTRAAGDPARPED
                     DEERVLDRRFWLPDTRTFGVGGRVSAAGYDLPEPVVDRLAGAPGDVVARSSSRAFDSL
                     ALRASMALDGNRDTAWVPAGQGPGEWIDLSFPGRQLDHVVVRQDVPDALKGRKGANVA
                     VEADLSLDGGAPRPVRLRSGATRIDFAKRPVHRLRLELTKVAGLGGGVRISEIEAGGV
                     RVPPAKPGPLQGCATIARL"
BASE COUNT          294 a         1248 c         1194 g          412 t
ORIGIN      
        1 cctcgcgctc gccgccctgc gtggcctcgg cgcgggcccg tggctcgccg tgcgcctgtg
       61 gcgggcgctc ctcctgctcg tcgccgggtg gggagcagcc cggctgttcg cggacgtgac
      121 ccgcgacgag acggcggcgg acgcggccgg caggggccgt gccggcgcgg ccggcagggt
      181 cgccgccgcg gtcttctacg tcgccaaccc ctacgtggtc gtggccgggg cgaccgaccc
      241 gatcctcgtg ccgtacgcgt tcctgccctg gctcgtcctg gcgctcgccc gcagcgtgcg
      301 ccggccccgg tcgtggcggg cgcccgcggc gttcgcgctc ggcttcgcgc tctgcggcgg
      361 gatgaacgcg ggcgtcgtgc cgctgttcat gctgcttgcc gtcccctgct acctcgccta
      421 cgccaggcgg gcgctcgggg tgggatggcg cgacctcggc gcggcggccg gccgctgcct
      481 cggcctggcg ctcctggtct cgctgtactg gctggtgcct gccgcgctcg ccagctcgag
      541 cggcgccgcg atcgccgcca ccaccgagac gccccgcgac gtggccgggc cgtcgtccta
      601 cgccgagacg ctgcgcctgc tcgggctgtg gaccctctac gggcaggcgg gggggcggcc
      661 cttcctgccc ggcatggtcg cctacctgac caacccgctg gtcgtgctcg cctccttcgc
      721 cctcccggtg gccgcgaccg cctcggccct cgcctcgcgg gcacgggcga ggctgctccc
      781 ggccgccctg ctcgcggcgg ccgtccccgt gatggtcggg ctgttcccga ccgccgggcc
      841 ctcgccgttc gggcgggtcc tcgaggcggc gttccggagg ctgccaggcg cgatcgcgtt
      901 ccggaccacc aacaaggtcg gcgccctggt cgcgctcgcc ctgaccctgc tgctggcgct
      961 cggggccgcc gagctggccg gctgggcgga ggccaggagg tcgtccggtc ccttgagccc
     1021 tcagcggagc gccgggtggg ccgggccccg ctggctcggg gcggccgggc ggccgccgtc
     1081 cgggcgcctg gccggcggca tcggcgccgc gatggcggcc gtcctcgccc tcgccgtcct
     1141 gcccgcctgg accggcggtc tcaacctcgc gcgctaccgc gtccccgggt actggcgaca
     1201 ggcggccgcc gacctgaacc gcggcccggc gtccagccgg gtgctcgtgc tgccgggcca
     1261 ggtgcaggca gactaccgct gggggctcga ggggcccgac gacctcgacg cgtccctgct
     1321 gacgcggccg tcggtggtgc gctccacggt cccgaacggc tccaaggagc aggccaactt
     1381 cctcgccgcc ctcgacctcg cgctcccgcc cacctcgccc gataccgcgg gggtcggcac
     1441 catggcccga tacctgggcg ccggcgaggt gctcgcccgc tacgaccggg tctgggaggc
     1501 ggccgggggc acgccgccct cggtgctcgc ggccgcgctc cgccaggatc cgggcctgcg
     1561 cagggaagcc gcctacggcc ggccggggga gcacacggtc gcgccgcacg ggggcgcgcc
     1621 cggggatgcc gggcttgcgc cgctcgagcg cttcgcggtc gccggcgctc ggccggtcca
     1681 gcggaccgag cccgcgcagg ggacgctgct gatcgacggc gacagcttcg ccctgccccc
     1741 gctcgaccgc ctcgggcggc tccagggcca acccccgttc cggctgctcg gctcgaccag
     1801 cctcgacgag gcggcgctgg ccctggacga cggggcgcgc atcgtgctca ccgacacgaa
     1861 ccggcggcgg gagtgggatc cccaccgcag cggcccgtcc ttctcgccga cgctgcgcgc
     1921 cgaccagccc atcgccgacg gcgcgtcctc cctcaccttg tcaggcgacc cgttgtcagg
     1981 cgacccgtcg tcaggcgacc cggccgacca gagcgtcgcc gtcctcgacg gcgcccgcgc
     2041 ggtcaccgcg accagctcgg gccccgcgtt cgggctggcc ccctggggca agccgtcgtt
     2101 cgcgttcgac ggcgacccgc ggacggcctg ggtcaccggc gcctacggga cggcggtggg
     2161 ccagtccatc acgatcgagt tcggccggcg ggtgcggatc tcccgcctga ccctccgccc
     2221 cctgctcggc tcgccggccc aggtctcgag cgtgcggatc tcgctcgacg gccgctcgtg
     2281 ggacgccgag gtcccggccc ggccggaggt gaccgtgccg gtgcccgaga cggacgcgta
     2341 cgccgccacc gtcgagatca ccggggtgcg gggcggcgcg ggcctgaacc cggtgggctt
     2401 ctatgagatc ggcatcccgg gcgtgcgggc caccgagggc gtgcgcatgc ccgagcgctt
     2461 ccgcgagctc gccgggtcgc tcgacaccgg cgcggggaag cgcctcgccg ccaccccgat
     2521 cgaggtcgtc atgacgaggg cggccggcga cccggcccgg cccgaggacg acgaggagcg
     2581 ggtgctcgac cgccgcttct ggctgccgga cacgcggacg ttcggggtcg gcggccgggt
     2641 ctcggccgca ggctacgacc tgcccgagcc ggtcgtcgac cggctcgcgg gcgcgcccgg
     2701 cgacgtggtc gcgcgctcct cgtcgcgggc cttcgactcg ctcgcgctgc gcgcctccat
     2761 ggcgctcgac ggcaaccggg acaccgcctg ggtgcccgcc ggccaggggc ctggggagtg
     2821 gatcgacctc tcgttccccg ggcggcagct cgaccacgtg gtcgtcaggc aggacgttcc
     2881 cgacgcgctc aaggggcgca agggcgcgaa cgtcgccgtc gaggccgacc tctcgctcga
     2941 cggaggggct ccccgaccgg tgcgcctgcg ctcgggggcg acacggatcg acttcgcgaa
     3001 gcgcccggtg caccggctgc ggctggagct caccaaggtc gcgggcctcg ggggtggggt
     3061 gcgtatcagc gagatcgagg ccggcggcgt ccgcgtcccg cccgcgaagc ccgggccgct
     3121 gcagggctgc gcgacgatcg cccggctc
//
LOCUS       DASPYY010000079         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_293889, whole genome shotgun sequence.
ACCESSION   DASPYY010000079 DASPYY010000000
VERSION     DASPYY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1447)
                     /locus_tag="VEP73_01075"
     CDS             complement(173..1447)
                     /locus_tag="VEP73_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HYT25065.1"
                     /translation="MLNARLDALLDEPLDWRDRAVPGTGGAVTLGTMGAQGWNLLAGD
                     LLLPVVVLKADALEHNLRTMARWCADHGVLLAPHGKTTMAPQLVERQLDLGAWGVTAA
                     TAAQARVFRAFGVERILLANELVDPAALGWVARELAADPGFWFSCLVDSEAGVALMDG
                     ALARAGAERPLPVLVELGAPNARAGCRTDAEAAAVAAAVARSRYLELAGVEGYEGSVR
                     ADGSGPVQAAVDRFLDRLRAFTTDLDRQGAFDHADEVIVTAGGSAFFDRVAERLAFPA
                     GRRPVRVVLRSGCYVTHDHGRYARVSPLREGAAPTPLRPALELWGEVLSRPEPGLAIV
                     GFGKRDAPYDLDLPIPLAVVRRSGERPGAEGLRVVELNDQHAFVRVEGELGVGDLVGC
                     GISHPCTTFDKWRLIPLVDDGYRVTGGIRTFF"
     gene            complement(1538..>2969)
                     /locus_tag="VEP73_01080"
     CDS             complement(1538..>2969)
                     /locus_tag="VEP73_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HYT25066.1"
                     /translation="KLSWVVTAYLLTSTSATPLWGKISDLYGRRLIFQAAIAIFLVGS
                     MLSGLSQNMTELIAFRAVQGIGGGGLFSIALSIIGDVIPPRERGRYQGYFGAVFGISS
                     VAGPLLGGWFTDGPGWRWIFYINLPIGLAALAVTTMALRMPVVRRHHRIDFAGAATIV
                     AAVTCLLLYLDWRGNSYGWTEGGALALLAGFMVFSLLFVWIELHTAEPIIPMRLFRNP
                     VFAVGNAYGFLAGVAMFGAIIFLPIYLQAVRGLSPTASGLSLLPAIIGIFATSITSGR
                     LITRTGRYRIYPIIGSVVLVIALFLLSRLGVDTPAWQVGLYEFLFGAGLGFTLQTITT
                     AVQNAVDFRDLGTATAAVTFFRQMGGAIGAAVFGAVLSSRLTHYLAEAFAGAPAGAGR
                     GAGLDVNDIQAIQRLAEPAKSIVLHAFTNALDDVFLVGVPFIVVAFVVSLFMKEVPLR
                     TGPGTPTGGPTAPGAEDVPAPPLGGV"
BASE COUNT          454 a         1060 c         1125 g          330 t
ORIGIN      
        1 ccatcttccc gctcccgccg ctcccggtga agccgtcggg tcgccgcggc tcgcggcgcg
       61 acgcggcgat cgtgtcgaga tggctccgcc caagatcgcg gctccggtag cagcgcccgg
      121 ctgggacggc ccctgcgaga cgggcgcacg gtcgcccgcc cgttgcgacc cgtcagaaga
      181 acgtcctgat cccccctgtg acccggtacc cgtcgtcgac cagcgggatg agccgccact
      241 tgtcgaaggt ggtgcacggg tgggagatcc cgcagcccac gaggtcgccg acgccgagct
      301 ccccctccac ccgcacgaag gcgtgctggt cgttcagctc gaccacccgc aggccctcgg
      361 cccccgggcg ctccccggag cggcggacga ccgccagggg gatcggcagg tcgaggtcgt
      421 agggcgcgtc gcgcttgccg aagccgacga tggcgaggcc cggctcgggc cgggacagca
      481 cctcgcccca gagctcgagc gccggccgca gcggcgtggg ggcggcgccc tcgcgcagcg
      541 gtgagacgcg cgcgtaccgc ccgtggtcgt gggtgacgta gcagccgctg cgcagcacga
      601 cccggaccgg ccggcgcccg gccgggaagg ccagccgctc ggcgacccgg tcgaagaagg
      661 cgctcccgcc cgcggtcacg atcacctcgt cggcgtggtc gaaggcgccc tgccggtcga
      721 ggtcggtggt gaaggcgcgc aggcggtcga ggaagcggtc gacggcagcc tggaccggcc
      781 cggagccgtc ggcgcggacg gagccctcgt agccctcgac gccggcgagc tcgaggtacc
      841 gcgagcgggc cacggcggcg gcgacggcgg ccgcctccgc gtcggtccgg cagccggccc
      901 gcgcgttcgg cgcccccagc tcgaccagca cgggcagggg ccgctccgcc ccggcccgcg
      961 ccagggcgcc gtccatcagc gcgacgcccg cctccgagtc gaccaggcag ctgaaccaga
     1021 agcccgggtc ggcggcgagc tcccgggcga cccacccgag cgcggccggg tccaccagct
     1081 cgttggcgag caggatgcgc tcgaccccga aggcgcggaa cacccgggcc tgggcggccg
     1141 tggcggcggt gacgccccag gcgccgaggt cgagctggcg ctcgacgagc tgcggcgcca
     1201 tcgtcgtctt cccgtgaggg gccagcagga cgccgtggtc ggcgcaccag cgggccatgg
     1261 tgcgcaggtt gtgctcgagc gcgtcggcct tcaggaccac gaccggcagg agcaggtcgc
     1321 cggcgagcag gttccacccc tgcgcgccca tggtcccgag cgtcaccgcc cctccggtgc
     1381 cgggcacggc gcggtcgcgc cagtccagcg gctcatcgag cagggcgtcc agccgggcgt
     1441 tcagcatgac cgtacgatac cgcccatgcg gacgtcagcc ttccggcggc gtgcgccatc
     1501 ccagcgcacc ggctggccgc gggatcaggg cgcaggctca gacgccgccg agcggcgggg
     1561 ccggcacgtc ctcggctcct ggggcggtgg gaccgccggt cggcgtgccc ggaccggtgc
     1621 gcagcggcac ctccttcatg aacagcgaca cgacgaacgc gacgacgatg aacggcaccc
     1681 cgacgaggaa gacgtcgtcc agggcgttgg tgaaggcgtg caggacgatg ctcttcgccg
     1741 gctcggccag ccgctggatc gcctggatgt cgttgacgtc gagccccgcg ccacgccccg
     1801 cgccggcggg cgcgccggcg aacgcctcgg ccaggtagtg ggtgagccgg ctcgacagga
     1861 ccgcaccgaa cacggccgcg ccgatggcgc cgcccatctg ccggaagaag gtgaccgcgg
     1921 ccgtcgccgt gccgaggtcg cggaagtcca ccgcgttctg gaccgcggtg gtgatcgtct
     1981 gcagcgtgaa gccgaggccg gcgccgaaga ggaactcgta gagcccgacc tgccaggccg
     2041 gcgtgtccac gccgaggcgc gacagcagga acagcgcgat gacgagcacc acggagccga
     2101 tgatcgggta gatcctgtac ctgccggtgc gggtgatcag ccggcccgag gtgatcgagg
     2161 tggcgaagat gccgatgatg gcgggcagca gcgacaggcc cgaggcggtg ggcgacaggc
     2221 cccgcaccgc ctgcaggtag atcggcagga agatgatcgc gccgaacatc gcgacgccgg
     2281 cgaggaagcc gtaggcgttg ccgacggcga acaccgggtt gcggaacagc cgcatcggga
     2341 tgatcggctc ggcggtgtgc agctcgatcc acacgaagag cagcgagaac accatgaacc
     2401 cggcgagcag cgcgagcgcg ccgccctcgg tccacccgta ggagttgccc cgccagtcga
     2461 ggtagaggag caggcaggtc acggcggcca cgatcgtcgc cgcgccggcg aagtcgatcc
     2521 tgtggtggcg acggaccacg ggcatccgga gcgccatcgt ggtcacggcg agcgcggcga
     2581 ggccgatcgg caggttgatg tagaagatcc agcgccagcc cggcccgtcg gtgaaccagc
     2641 cgccgagcag cgggccggcc acgctcgaga tgccgaagac cgccccgaag tagccctggt
     2701 accggccgcg ctcccggggc gggatgacgt cgccgatgat gctgagcgcg atcgagaaga
     2761 ggcccccgcc gccgatgccc tggaccgcgc ggaaggcgat cagctcggtc atgttctggc
     2821 tgaggcccga cagcatcgag ccgacgagga agatcgcgat ggcggcctgg aagatcagcc
     2881 tgcggccgta gaggtcggag atcttgcccc acagcggggt ggccgacgtc gaggtcagca
     2941 ggtaggcggt gacgacccag gagagcttg
//
LOCUS       DASPYY010000080         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1137338, whole genome shotgun sequence.
ACCESSION   DASPYY010000080 DASPYY010000000
VERSION     DASPYY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137338"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEP73_01085"
     CDS             complement(<1..277)
                     /locus_tag="VEP73_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25067.1"
                     /translation="MLPPRVVRRLVLAPLPLLGTVVLLVLLPVWVLLAAATSPWLPGR
                     WRALRLTWFGLVWLALESAALFACLGLWVASGFGGRLRSERYQDRHYA"
     gene            complement(282..1166)
                     /locus_tag="VEP73_01090"
     CDS             complement(282..1166)
                     /locus_tag="VEP73_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYT25068.1"
                     /translation="MEDPGRRHGVAFVLGGGGVLGAHEVGMLQALAEAGIHADLVLGT
                     SVGAFNGVFVAADPAAAVSRLAQVWRDGAASEAFGGSLFGRVTTLARSGTHLHSNEPL
                     RRLLRELLPVARIEDLAVPFQCVAASIERAAAHWFTSGPIVPAVLASAAVPGLLPPVR
                     VGSEHFFDGGLVHSIPVGRAVKLGAHTVYVLHVGRIERPLEVPRRPWEVGLVAFEIAR
                     RHRFAEDMASLPRDVTVHVLPAGVNGQGRGADLAQLRYRDVSRIDEHITRAYEASADY
                     LAGWRDRRPPGPGRRPAG"
     gene            1368..1535
                     /locus_tag="VEP73_01095"
     CDS             1368..1535
                     /locus_tag="VEP73_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25069.1"
                     /translation="MPRVRRHHRRGRARNRNRRRSYWPRRSRRSQERLLLWIVIVGGL
                     VLLLLIALRSS"
     gene            complement(1556..>1808)
                     /locus_tag="VEP73_01100"
     CDS             complement(1556..>1808)
                     /locus_tag="VEP73_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25070.1"
                     /translation="RLLSNEPDDGELARVLPAARARVGDVSRVARTIRRAATAGLGAA
                     HAADVLALNAGVEREVAALDTGVETLVALAADQPAAWTA"
BASE COUNT          245 a          670 c          713 g          180 t
ORIGIN      
        1 tcgcgtagtg ccggtcctgg taccgctcgg agcgcaaccg gccgccgaag ccgctcgcga
       61 cccacaggcc caggcaggcg aagagcgcgg ccgactcgag cgccagccac accaggccga
      121 accaggtgag ccggagcgcc cgccagcggc ccgggagcca gggcgacgtc gcggcggcga
      181 ggagcaccca cacgggcagc aggacgagca ggaccacggt gccgaggagc gggagcggcg
      241 cgagcaccag ccgccggacg acgcgggggg gcagcatgcg gtcacccggc cggccggcgg
      301 ccggggcccg gagggcgacg gtcccgccag ccggcgaggt agtcggcgga cgcctcgtac
      361 gcacgcgtga tgtgctcgtc gatgcgggag acgtcgcggt agcggagctg tgccaggtcg
      421 gcgccgcggc cctggccgtt cacgcccgcg gggaggacgt ggacggtgac gtcccgcggg
      481 agcgaggcca tgtcctcggc gaagcggtgc cgccgcgcga tctcgaacgc gaccaggccg
      541 acctcccacg gccgccgcgg cacctccagg gggcgctcga tgcggccgac gtggagcacg
      601 tagacggtgt gggcgccgag cttcaccgcg cggcccaccg ggatgctgtg caccaggccg
      661 ccgtcgaaga agtgctcgct ccccacccgg accgggggca gcaggccggg cacggcggcc
      721 gaggcgagca ccgccgggac gatcggcccg ctggtgaacc agtgcgccgc ggcccgctcg
      781 atgctcgcgg cgacgcactg gaacggcacg gccaggtcct cgatgcgggc gacgggcagg
      841 agctcacgga gcagccggcg cagcggctcg ttggagtgga ggtgggtgcc tgagcgcgcg
      901 agcgtcgtga cccggccgaa cagcgagccg ccgaacgcct cgctggcggc gccgtcccgc
      961 cacacctggg cgagccgcga caccgccgcg gccgggtcgg ccgcgacgaa gacgccgttg
     1021 aacgcgccga ccgaggtgcc gaggacgagg tccgcgtgga tcccggcctc ggcgagcgcc
     1081 tgcagcatgc cgacctcgtg cgcgccgagg acgccgccgc cgccgagcac gaacgcgacc
     1141 ccgtgccgcc gtcccgggtc ctccacaccg tctccttccg tgtcgcgcga tgatcccacg
     1201 gtggcggccg ccgctcacgc gggagccgcc cgacctcctc gccggagggt gcgccgcagg
     1261 gtggggagca gcgcggccac gagcacccac cctccggttg acgggcccga gtcgtgggga
     1321 tacggggatc gagcgcgcac cgtcgcggga cacgaaggag gcaccccatg ccgcgcgtga
     1381 gacgccatca ccggcggggc cgggcccgca accgcaaccg ccgccggtcg tactggccgc
     1441 gccggtcgcg ccggtcgcag gagcgcctgc tcttgtggat cgtgatcgtg ggcggtctgg
     1501 tcctgctgct gctgatcgcc ctcaggtcgt cgtagaccgc cgccataccg gcggctcagg
     1561 ccgtccaggc tgccggctgg tcggcggcca gggcgacgag cgtctccacc ccggtgtcga
     1621 gcgccgccac ctcgcgctcg acaccggcgt tgagcgcgag cacgtcggcg gcgtgcgcgg
     1681 cgccgagacc ggcggtggcg gcgcgcctga tggtccgggc gacgcggctg acgtcgccga
     1741 cgcgggcgcg cgcggcgggc aggacgcgtg ccagctcgcc gtcgtcgggc tcgttcgaca
     1801 agagccgg
//
LOCUS       DASPYY010000081         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_996993, whole genome shotgun sequence.
ACCESSION   DASPYY010000081 DASPYY010000000
VERSION     DASPYY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996993"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VEP73_01105"
     CDS             complement(<1..1551)
                     /locus_tag="VEP73_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT25071.1"
                     /translation="MAGLDLLEGTILAGYRIVAPIGRGGMGVVYQAEEAALGGRTVAL
                     KVLSPALSHDGGFRRRFLREMRLASSIEHPNVIPVYRAGEEDGLLYIAMRYVRAADLR
                     ILLGREGRLDPGHALAIVEQVARALDAAHARGLVHRDVKPANVLLGPPAGRGEPEHVY
                     LVDFGLARSAEPDASITGGGSFMGTPRYAAPEQIAGQAVDGRTDVYALGCVLYECLTG
                     RPPFTADDGLAVLHAHMWADVPRISSERPGLPPELDGVVGRALAKAKEDRFPSCGELV
                     AAARRALARPGQPAPGWPAPVPATRAEPVPATRAAEAPTRVVSRPPGALVDYQPLGRP
                     ARRARRGLAAAVFLAFAGMLVNLDETELGRWLRGRPPAAGPGGPAVAVSVLQALVFLV
                     TAALFLVWFGRAYANLAVLGARGLRYRRWWATAGWLLPVYSLFRPKQVLNDVWRASDP
                     ELPAEAGTSWRGERVPALLGWWWLAFLASTLARSVTVESVHALASVMTFGLSRRLDAV
                     APSAGAQAT"
     gene            2082..2363
                     /locus_tag="VEP73_01110"
     CDS             2082..2363
                     /locus_tag="VEP73_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25072.1"
                     /translation="MAAGFAALALGVLLGAATGGVAVTPAGATPPAAVTVTTTVTKAV
                     VPSVCLSAMRQGDAVIGLLDKDERDRQLGELLKSYTVAAQSCRKEVSRR"
     gene            2360..2854
                     /locus_tag="VEP73_01115"
     CDS             2360..2854
                     /locus_tag="VEP73_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HYT25073.1"
                     /translation="MRTRRPLKALLVLAALVALGAAPASAAQTLVLTCTICSQVIATG
                     SGLPANSTVRLTMTDVVTGQEVAPQIPVQTDAQGKFLKKVNVDLYKHPSLESSVWASN
                     GQVLVIAAHNRFNSPCKGHSMDMGNMLPMTGSSHAPVFLGISLALLAVGAVLLGAARR
                     RTAP"
     gene            3033..>3363
                     /locus_tag="VEP73_01120"
     CDS             3033..>3363
                     /locus_tag="VEP73_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT25074.1"
                     /translation="MSLTVLMNAGPWLAVPPRHYGGTENVVACLIPELRRRGVRVVLC
                     TVGESTVAVDRRRVSFPEGQFRHLGGAYGQVVGITHSHMGHVLDELRHQRVDLVHDHL
                     EVVGPSML"
BASE COUNT          470 a         1296 c         1214 g          383 t
ORIGIN      
        1 ggttgcctgg gcgccggccg acggcgccac ggcgtcgagc cgccgcgaca ggccgaaggt
       61 catcaccgag gcgagcgcgt gcaccgactc gacggtcacg ctgcgagcca gcgtggaggc
      121 gaggaacgcg agccaccacc agccgagcag ggcgggcacg cgctcgcccc gccacgacgt
      181 ccccgcttcg gcgggcagct cggggtcgct ggcccgccag acgtcgttca gaacctgctt
      241 gggccggaac aggctgtaga ccggcagcag ccagcccgcg gtcgcccacc agcgccggta
      301 gcgcaggccg cgcgcgccga ggacggccag gttggcgtac gcgcgcccga accagacgag
      361 gaacagcgcg gcggtgacca ggaacacgag cgcctggagc acgctgacgg cgaccgccgg
      421 cccgcccggc cccgcggcgg gcggccgccc gcgcagccac cggccgagct cggtctcgtc
      481 gaggttgacc agcatgccgg cgaaggcgag gaacaccgcc gccgcgaggc cgcgccgggc
      541 ccgccgcgcc ggccgcccga gcggctggta gtcgacgagt gcgcccgggg gccgggagac
      601 cacccgcgtc ggcgcctccg ccgcccgggt ggccggcacc ggctccgccc gggtggccgg
      661 caccggtgcc ggccacccgg gcgccggctg cccgggccgg gcgagcgcgc ggcgggcggc
      721 ggccaccagc tccccgcacg acgggaagcg gtcctccttg gccttggcca gcgcccgccc
      781 gaccaccccg tcgagctcgg gcggcaggcc cggccgctcg gagctgatcc tcggcacgtc
      841 cgcccacatg tgggcgtgca ggacggccag gccgtcgtcg gccgtgaacg gcggccggcc
      901 ggtgaggcac tcgtagagca cgcagccgag cgcgtagacg tcggtccgcc cgtcgaccgc
      961 ctggcccgcg atctgctccg gcgccgcgta ccgcggcgtg cccatgaacg acccgccccc
     1021 cgtgatcgag gcgtcgggct ctgccgacct ggccagcccg aagtcgacga ggtacacgtg
     1081 ctctggctcg ccccgcccgg ctggcggccc gagcagcacg ttggccggct tgacgtcccg
     1141 gtggaccagc ccgcgggcat gggcggcgtc gagggcgcgc gccacctgct cgacgatggc
     1201 gagggcgtgg ccggggtcga ggcggccctc acgcccgagc aggatgcgga ggtccgccgc
     1261 ccgcacgtag cgcatcgcga tatagagcag cccgtcctcc tcgccggcgc ggtagacggg
     1321 gatgacgttc gggtgctcga tcgaggaggc gagccgcatc tcgcgcagga accgccgccg
     1381 gaagccgccg tcgtgggaca gggcggggga caggaccttg agggccaccg tccggccgcc
     1441 gagcgcggcc tcctcggcct ggtagaccac gcccatcccg ccccggccga tcggcgccac
     1501 gatccggtac ccggcgagga tggtgccctc gaggaggtcg agaccagcca tcgccacctc
     1561 agtccgcgtc cctcggcgtc acgttcgagc aggtccaacc tacccgccga cgcggcaccg
     1621 caaccgccgc agccgagccc gcccggcatt tgcggcggag cgaccgaaaa gcatccgtaa
     1681 cgggcacggc ggggcgtccg gacgggcatc gcggcgcgtt ccgcgctgaa acgaatcgaa
     1741 taggtcgttg tgcagggtct gggcacgaat atggcagctc cgcactattg ccgaattcac
     1801 gaggggccgc cgccgaaata ggctcccggc cgcggcgacc tcgcgtcgcg gctgtcgggc
     1861 gccggtgccc gccatgcctc gcgggtcgcg cggacgtgca cggacgacga gcgagggggg
     1921 ccatgccgat gctgcgccat ggacggcgct aggagcagat ggcacatgcg cgctcgccgt
     1981 gtcgcgcgcg gaatcggaag ggtggcgcca tgcacccgac aggacgcgat cccacggagg
     2041 accctgaaca attcccaccc cgccgccccc gctggtggcc ggtggccgcc ggcttcgccg
     2101 cgctcgccct cggggtgctg ctcggcgccg ccaccggagg cgtcgcggtg acccccgccg
     2161 gggcgacgcc gccggccgcc gtcacggtga ccacgacggt cacgaaggcg gtggtgccga
     2221 gcgtctgcct gtccgccatg cggcagggcg acgccgtcat cggcctgctc gacaaggacg
     2281 agcgtgaccg ccagctcggc gaactgctca agtcgtacac cgtcgcggcc cagtcgtgcc
     2341 gcaaggaggt atcccggaga tgagaacccg ccgcccgctg aaggccctgc tcgtcctcgc
     2401 ggcgctcgtc gccctgggcg ccgcgcccgc gagcgcggcc cagacgctgg tgctgacctg
     2461 caccatctgc tcgcaggtca tcgccaccgg cagcggcctg cccgccaact cgacggtccg
     2521 cctgaccatg accgacgtgg tgacgggcca ggaggtcgcg ccgcagatcc cggtccagac
     2581 cgacgcccag ggcaagttcc tcaagaaggt caacgtcgac ctgtacaagc acccctcact
     2641 cgagtcgagc gtgtgggcga gcaacggcca ggtgctcgtc atcgccgcgc acaaccgctt
     2701 caactcgccc tgcaaggggc attcgatgga catggggaac atgctcccga tgaccggctc
     2761 ctcccacgct ccggtgttcc tcggcatctc cctggccctc ctcgccgtcg gtgcggtgct
     2821 cctcggcgcg gccaggcgcc ggaccgcccc ctgaggcgcg taggctgatc tcccgccgcc
     2881 ggggccgagc ccgccccggc caccctgcgc ccggtttgcc gaccgacccc gctggcaacg
     2941 ctcggcaggt cgcacgcgaa ccgggcgccg gcacgccagg gcatcggccg gcacgccagc
     3001 cccaggcacc ggaccagggc atcggaggga cgatgagcct caccgtactg atgaacgcgg
     3061 gcccgtggct cgcggtgccg ccgcgccact acggcggcac cgagaacgtg gtcgcctgcc
     3121 tgatccccga gctgcgccgc cgcggcgtcc gcgtcgtgct ctgcacggtc ggcgagtcca
     3181 ccgtcgcggt ggaccggcgg cgcgtgtcgt tcccggaggg acagttccgg catctcggcg
     3241 gcgcctacgg gcaggtcgtc ggcatcaccc acagccacat gggccacgtc ctggacgagc
     3301 tgcgccacca gcgcgtcgac ctcgtccacg accacctcga ggtggtcgga ccgagcatgc
     3361 tcg
//
LOCUS       DASPYY010000082         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_293993, whole genome shotgun sequence.
ACCESSION   DASPYY010000082 DASPYY010000000
VERSION     DASPYY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293993"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEP73_01125"
     CDS             <1..971
                     /locus_tag="VEP73_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25075.1"
                     /translation="YLLGGAAPDGTVITPKYVGSRRLIEVAVATLATDRALLLLGVPG
                     TAKTWLSEHLAAAVSGDSTLMVQGTAGTAEEAVRYGWNYARLIAEGPSRDALVPSPVM
                     RAMAGGQIARVEELTRMPSDVQDALITILSEKTLPVPELGTEQQAVRGFNVIATANDR
                     DRGVNDLSSALRRRFNTVVLPVPASAEQEVDIVARRVGELGRSIGLPETPHGADEIRR
                     VVTVFRELRQGVTEDGRAKVKSPSGTLSTAEAISVVTSGLALAAHFGDGVLRPADVAG
                     GITGAVVQDPVADRAVWQEYLETVVRDRDDWSDFYRACRDVAGPND"
     gene            964..>1954
                     /locus_tag="VEP73_01130"
     CDS             964..>1954
                     /locus_tag="VEP73_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HYT25076.1"
                     /translation="MTSTVSTAVYGIRHHGPGSARSVRAALAGQRPDVVLIEGPPEAD
                     DLVALAADPDMRPPVALLGYVPGEPRQAAFWPFAVFSPEWQAITYALDAGVPVRFCDL
                     PAAHQLALKAAAPEEDAAEDGEKPPRRRTDPVSDLAAAAGYDDPERWWEDVVEHVPGP
                     AVFDALAEAISILRDDAGETGSRDAVREAHMRRVLRRTVKDGFERIAVVCGAWHVPAL
                     RELPSAAADDRLLRGLPKVKAALTWVPWTYGRLSYASGYGAGIRSPGWYDHLFSSPGQ
                     PVERWLARAAAVLRADGIPASSAHVIESVRLAEALAALRGRPLAGLEEVTEAAH"
BASE COUNT          245 a          698 c          758 g          253 t
ORIGIN      
        1 tctacctgct gggcggcgcg gcccccgacg gcaccgtgat cacgccgaag tacgtcggtt
       61 cccgccgcct catcgaggtc gcggtggcca cgctggctac ggaccgggcg ctgctgctgc
      121 tcggcgtgcc gggcacggcg aagacgtggc tgtccgagca cctggccgcg gccgtctccg
      181 gcgactccac gctaatggtg cagggcacgg cgggcaccgc cgaggaggcg gtcaggtacg
      241 gctggaacta cgccaggctg atcgccgaag ggccgtcacg ggacgcgctg gtgccgagcc
      301 cggtgatgcg ggcgatggcg ggcggccaga tcgcccgggt cgaggagttg accaggatgc
      361 cgtcggacgt gcaggacgcg ctgatcacca tcttgtcgga gaagacgctg ccggttcccg
      421 agctcggcac cgagcagcag gcggtccgcg gcttcaacgt gatcgccacc gccaacgacc
      481 gggaccgcgg cgtgaacgac ctgtccagcg ccctgcgcag gcggttcaat accgtggtgc
      541 tcccggtgcc cgcctcggcc gaacaggagg tcgacatcgt cgcccgccgg gtcggcgagc
      601 tcggccgctc gatcgggctg ccggagacgc cgcacggggc ggacgagatc aggcgggtgg
      661 tcacggtgtt ccgcgagctg cgccagggcg tcaccgagga cgggcgggcc aaggtcaagt
      721 cgccgagcgg cacgctgtcc acggcggagg cgatctccgt ggtcaccagc ggcctggcgc
      781 tggccgcgca cttcggcgac ggggtgctgc gcccggccga cgtggccggc ggcatcaccg
      841 gcgccgtcgt gcaggatccg gtcgccgacc gcgcggtgtg gcaggagtac ctggagacgg
      901 tcgtcaggga ccgcgacgac tggagcgact tctaccgcgc ctgccgtgac gtcgccggcc
      961 cgaatgacta gcactgtcag caccgccgtc tacgggatcc gccatcacgg gcccggatcg
     1021 gcgcggtcgg tgcgggccgc cctggccgga caacggcccg acgtcgtcct gatcgagggt
     1081 ccgccggagg cggacgacct ggtcgcgctg gccgcggacc cggacatgcg ccccccggtg
     1141 gcgctgctcg gttacgtgcc cggggagcca cggcaggcgg cgttctggcc gttcgcggtg
     1201 ttctcgccgg aatggcaggc gatcacgtac gcgctggacg cgggggtccc ggtccggttc
     1261 tgcgacctgc ccgccgcgca tcagctcgcc ctcaaggcgg cggccccgga ggaggacgcg
     1321 gcggaggacg gggagaagcc gccgaggcgg cgcaccgacc cggtgagcga cctggccgcc
     1381 gcggccgggt acgacgaccc ggagcgctgg tgggaggacg tggtggagca cgtccccggg
     1441 cccgcagtct tcgacgcgct ggccgaggcc atctcgatcc tgcgggacga cgccggggag
     1501 accggctcgc gggacgcggt ccgcgaggcg cacatgcgcc gggtcctgcg ccggaccgtc
     1561 aaggacgggt tcgagcggat cgccgtggtc tgcggcgcct ggcacgtgcc ggccctgcgg
     1621 gagctgccgt ccgcggccgc ggacgaccgc ctgctgcgcg gcctgccgaa ggtgaaggcc
     1681 gccctgacct gggtgccgtg gacctacggg cggctgtcct acgcctcggg ctacggggcg
     1741 ggcatccgct cccccggctg gtatgaccac ctgttctcct cgcccggcca gccagtcgag
     1801 cggtggctgg cccgggccgc cgccgtcctg cgcgccgacg gtatcccggc gtcatcggcg
     1861 cacgtgatcg agtccgttcg gctcgccgag gcgctcgccg ccctgcgcgg ccgtccgctg
     1921 gccggcctgg aggaggtcac cgaggccgcc cacg
//
LOCUS       DASPYY010000083         7888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_575802, whole genome shotgun sequence.
ACCESSION   DASPYY010000083 DASPYY010000000
VERSION     DASPYY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575802"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VEP73_01135"
     CDS             complement(<1..664)
                     /locus_tag="VEP73_01135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYT25077.1"
                     /translation="MPLTDQTLALAEELIARYPERRSALLPILFLLQAEDGYVSPAGV
                     AQAAGLLGLTRAEVGAVATFYTMFRRRPVGKYLVSVCRTLSCQLRGSREIAEALSERL
                     GVPLGGTEPSGMVTVEEVECLAACDGAPVVQVNTENYERLTAGQAVQLVEALLHDEVP
                     PPTFAAEGSVTEPSTAAMHWRLAGLGEPPPSLGGRRPEEVAAVPTAFPPQPPAPTVDE
                     DPT"
     gene            complement(699..1964)
                     /locus_tag="VEP73_01140"
     CDS             complement(699..1964)
                     /locus_tag="VEP73_01140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYT25078.1"
                     /translation="MGGGDWAEVTARAHAEGDDDLMVINMGPQHPSTHGVLRLVLELR
                     GEEVVSCQPVVGYLHTGIEKTAEFRTWVQGVTLVTRMDYLAPIFNETAYCLAVEKLLG
                     IEAPPRAQAIRVLMMELNRVASHLVWLATTGLELGALSVMLYGFREREEILDILEDVT
                     GLRMNHAYVRPGGVIQDLPDGVDAKIRTFVGDMRAKADEYETLLTRNPIWLERTRGIG
                     HLTQDQLIDLGTTGPMLRAGGLAHDLRKAEPYCGYEQYDFEVPSATGSDVYARYQVRL
                     AEIRQSLRIVEQVLDSLPDGPVMIEDPKVGWPARLALGPDGLGNSLAHVNHIMGESME
                     ALIHHFKLVTEGFKVPAGEVYVTVESPRGELGYHVVSDGGNLPYRVHVRDPSFVNLQT
                     GGPMVEGLLLADVISAMSSIDPVLGGVDR"
     gene            complement(2114..2677)
                     /locus_tag="VEP73_01145"
     CDS             complement(2114..2677)
                     /locus_tag="VEP73_01145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYT25079.1"
                     /translation="MQPRDAVETPPDALQPVLTGLRERFGAAELQVTGYRGEVTVELD
                     RANLVEVATWLRDDPAARFRHLSDLSGTDYPDRPDRLCVDYHLYSFEHNARLRLKVRV
                     TVDDPHVPTLTGVWRTADWHEREVYDFFGVRFDGHPNLVRIEMPEDWKGHPQRKDYPL
                     GGVNVDYIGAHVPPPDTRRYKGGWTST"
     gene            complement(2770..3228)
                     /locus_tag="VEP73_01150"
                     /pseudo
     CDS             complement(2770..3228)
                     /locus_tag="VEP73_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(3246..3608)
                     /locus_tag="VEP73_01155"
     CDS             complement(3246..3608)
                     /locus_tag="VEP73_01155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYT25080.1"
                     /translation="MSLGSFTPILAMMVLATAFALVSIVVSAFLAPHRPTRAKYSAYE
                     SGIEPGRLPKGERFPVKFYLTAMLFVVFDVEVIFLYPWAVVFRQLKVFGLVEMGIFIG
                     LVLVAYVYDWKRGGLEWD"
     gene            complement(3733..4965)
                     /locus_tag="VEP73_01160"
     CDS             complement(3733..4965)
                     /locus_tag="VEP73_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HYT25081.1"
                     /translation="MTSQYDALVVGGGPGGAATAYHFARAGARVLLVEKAGYPRDKVC
                     GDGLTPRAVAALDTMGLRDEYRTWSRSAGLKVHGGGLVLELPWPELSRYPSYGLARPR
                     TDFDQLLARHAEKAGATLWESTEVVLPLFERGLVRGAVVRREGEDPVDVHAEVVVAAD
                     GASSRFAQTLGLHRDPGRPVGVAIRQYFTSERDHDPWLDSYLELWRGDDLLPGYGWVF
                     PMADGTVNVGLGLLNTSDHFRNTNYRRLLADWVPTVGRDWGFTPTDAVGGPRSAPLPM
                     AANRHPPLHRGVMFVGDAAGLVNPFNGEGIDYAMESGQLAAETGLAVLASGDRTLLRR
                     YRSGVELRYGSYFALGRAFVRAIGDPRVMKLATTYGLPRPALMKIVLKLLANLYEPAG
                     GDAADRVVRALTWMAPSR"
     gene            5208..5390
                     /locus_tag="VEP73_01165"
     CDS             5208..5390
                     /locus_tag="VEP73_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25082.1"
                     /translation="MRQKRRPPGAPREVRSIEQARCCATDGCEVHLSRYNPTPHCGVH
                     RGWDHEEVHRKPRHAG"
     gene            complement(5404..6141)
                     /locus_tag="VEP73_01170"
     CDS             complement(5404..6141)
                     /locus_tag="VEP73_01170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT25083.1"
                     /translation="MSASPAAERTSAGVRRMFDRVAPRYDLVNTVFSLGQDRAWRRAA
                     ALSTGLEAGETAVDVACGTGALTRELAGLAPGAVVLGLDFSWEMVRRGLPRPGGRGGG
                     DGRADIRRVRFAVADALRLPLADASADVLTIAFGLRNLPEPGQGLLEFRRVLRPGGRL
                     VVCEFSHPVVPVLDQVYGRYLTRLMPLAARRLSSDAEAYEYLARSIGAWPDQEGLARW
                     VREAGFERVRWRNLTGGIVALHWGAAA"
     gene            complement(6211..7062)
                     /locus_tag="VEP73_01175"
     CDS             complement(6211..7062)
                     /locus_tag="VEP73_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYT25084.1"
                     /translation="MRRSSLGVVLALALLVSAAVPAADAAAPAAPPPPGSVAAIGDSI
                     SQAFDACCWYGDHPSHSWSTGWFPFDGIASHYERIRALRPDVAGHWDLAVSGAKVADG
                     PRQAGEAVARRAGYVTVLLGANDLCAPSPDRMTPVPVFAERFRQTLAVLRAGLPAGAH
                     VFVSSIPDLHRLWEVLHGDPRARFVWRVAHICPALLARSNGPEERQAVAEREIAYNGV
                     LADACTGWSACRFDGLATYRYPFTADEVSALDYFHPDLKGQARLAEVTWRASFWASER
                     RTSAAVR"
     gene            complement(7175..7669)
                     /locus_tag="VEP73_01180"
     CDS             complement(7175..7669)
                     /locus_tag="VEP73_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYT25085.1"
                     /translation="MPNKVVPINEGAEAVRATLDDTDLAILRALQANARATFTEIGKA
                     VGLSAPTVHERVHRLEARGVIRAYRAEVDPDLLGPAVLSLVSVLPSDSSNAAAMSEVF
                     AGIEQIEAAYTVAGEESHVLVVRTRSVAELSEVLQRLRDVDGVARTRTAVVLSTPFQR
                     GPKL"
BASE COUNT         1131 a         2819 c         2881 g         1057 t
ORIGIN      
        1 cggtcgggtc ctcgtcgacc gttggcgccg gcggctgggg cgggaaggcg gtcggcacgg
       61 cggcgacctc ctcgggccgg cgcccgccga gcgacggcgg cggctcaccc agcccggcga
      121 gccgccagtg catggcggcg gtggacggct cggtgaccga cccctcggcg gcgaaggtgg
      181 gcggcggcac ctcgtcgtgc agcagggcct cgacgagctg gaccgcctgc ccggcggtca
      241 gccgctcgta gttctcggtg ttcacctgca cgaccggggc gccgtcgcag gcggcgagac
      301 actcgacctc ctcgacggtc accatgccgc tcggctcggt gccgccgagc ggcaccccga
      361 gccgctccga gagcgcctcc gcgatctcgc gcgagcccct gagctggcac gacagcgtcc
      421 ggcagaccga gacgaggtac ttgccgaccg ggcggcggcg gaacatcgtg tagaaggtgg
      481 cgaccgcgcc gacctcggcc ctggtgaggc cgagcagccc ggccgcctgc gccacccccg
      541 ccggggagac gtacccgtcc tcggcctgca ggaggaacag gatcggcagc agggccgagc
      601 gccgctcggg atagcgggcg atgagctcct cggcgagggc cagggtctgg tcggtcagcg
      661 gcacggcggt gggcttctcc ttgggcgctc ggcgtgggtc agcggtcgac gccgccgagg
      721 acggggtcga tcgaggacat cgcggagatg acgtcggcca ggagcaggcc ctcgaccatg
      781 gggcctccgg tctgcaggtt gacgaaggag gggtcgcgga cgtgcacccg gtagggcagg
      841 ttcccaccat cggacaccac gtggtagccg agctcgcccc gaggcgactc gacggtcacg
      901 tagacctcgc ccgccgggac cttgaagccc tcggtcacca gcttgaaatg gtggatcagg
      961 gcctccatcg actcgcccat gatgtggttg acgtgggcga gcgagtttcc caacccgtcg
     1021 gggccgagcg ccagccgcgc cggccagccg accttgggat cctcgatcat gacggggccg
     1081 tcgggcagcg agtccagcac ctgctcgacg atccgcaggc tctggcggat ctcggccagg
     1141 cggacctggt agcgggcgta gacgtcggag ccggtggcgc tcggcacctc gaagtcgtac
     1201 tgctcgtagc cgcagtacgg ctcggccttg cgcaggtcgt gggcgagccc gccggcgcgc
     1261 agcatcgggc cggtggtgcc gaggtcgata agctggtcct gggtgaggtg cccgatgccc
     1321 ctggtgcgct ccagccagat ggggttgcgg gtgagcaggg tctcgtactc gtcggccttg
     1381 gcgcgcatgt ccccgacgaa ggtgcggatc ttggcgtcga cgccgtcggg caggtcctgg
     1441 atgacgccgc cgggtcgcac gtacgcgtgg ttcatgcgca gcccggtgac gtcctcgagg
     1501 atgtcgagga tctcctcgcg ctcgcggaag ccgtacagca tcaccgacag cgcgcccagc
     1561 tccagcccgg tggtggcgag ccacaccagg tgggaggcga cccggttgag ctccatcatg
     1621 aggacgcgga tggcctgggc cctcggcggc gcctcgatcc cgagcagctt ctccaccgcc
     1681 aggcagtagg cggtctcgtt gaagatcggg gcgaggtagt ccatgcgggt gaccagggtg
     1741 acgccctgga cccaggtgcg gaactcggcc gtcttctcga tcccggtgtg caggtacccc
     1801 accaccggct ggcagctgac cacctcctcg ccgcgcagct cgagcacgag ccggagcacc
     1861 ccgtgggtgg acgggtgctg cgggcccatg ttgatgacca tcaggtcgtc gtcgccctcg
     1921 gcgtgggcgc gggcggtgac ctcggcccag tccccgccgc ccatggccag cacctgccgg
     1981 gcgcgccgct ggttgacggt gacgtgctcg gccggcgggg tggcgggcag ctcgagcgcc
     2041 tcacccggcc gcacgaccgc ctcgtcgacc agctccccgg tcggccggtc gatgctcggc
     2101 gtctcgagct cgctcatgtg ctcgtccacc ctcccttgta ccgcctggtg tccggcggtg
     2161 gcacgtgcgc gccgatgtag tcgacgttca ccccgccgag cgggtagtcc ttgcgctgcg
     2221 gatgcccctt ccagtcctcg ggcatctcga tgcggacgag gttcgggtgc ccgtcgaagc
     2281 gcacgccgaa gaagtcgtac acctcccgct cgtgccagtc tgccgtgcgc cagaccccgg
     2341 tcagggtcgg gacgtgcggg tcgtcgaccg tgacccggac cttgagccgc aggcgcgcgt
     2401 tgtgctcgaa cgagtacagg tggtagtcca cgcagagccg gtccggacga tccgggtagt
     2461 cggtccccga gaggtccgac aggtgccgga agcgggcggc cggatcgtcg cgcagccagg
     2521 tggccacctc gaccaggttg gcccggtcga gctcgacggt gacctcgccg cggtacccgg
     2581 tgacctgcag ctcggccgcg ccgaagcgct cgcgcaggcc ggtgaggacc ggctggaggg
     2641 cgtcgggggg cgtctcgacg gcgtcgcggg gctgcacctc gctcaggcct cctggtgggc
     2701 cgggaccgcc ccgcggccgt cgccgggccg gtcggtcagg acgagcttgg accggggctc
     2761 gcccttcctg atcttctcgt ggagccggat gatgccgtcc atcagcatct cgggccgggg
     2821 cgggcagcct gggacgtaca tgtcgacggg cacgatctgg tcgacgccct ggacgatcgc
     2881 gtagttgttg aacatgccgc cggaggtggc gcacacgccc atcgagacga cccacttcgg
     2941 ctcgggcatc tggtcgtaga tggtgcgcag caccggggcc atcttctggg acacccggcc
     3001 ggccacgatc atgaggtcgg cctgccttgg cgacgcccgg aacacctcca tgccgaaccg
     3061 ggccaggtcg tagcggccgg cgccggccgc catcatctcg atggcgcagc aggcgaggcc
     3121 gaaggtggcc ggccacagcg agcccttgcg ggcccagttg gccagcttct ccacggtggt
     3181 gacgaggatg ccgccaggca gcttctcgag cccgctaccg gtgctcatcg gtcagccctc
     3241 attggtcagt cccactccag tccgccgcgc ttccagtcgt agacgtaggc gaccagcacc
     3301 agcccgatga agatgcccat ctcgacgagg ccgaacacct tgagctgccg gaagacgacg
     3361 gcccacgggt acaggaagat cacctcgacg tcgaacacca cgaacagcat cgcggtgaga
     3421 tagaacttga ccgggaagcg ctcgcccttc gggagccgcc ccggctcgat gccggactcg
     3481 taggcggagt acttggccct ggtcggccgg tgcggcgcca ggaacgccga cacgacgatc
     3541 gacaccaggg cgaacgccgt cgcgaggacc atcatcgcga ggatcggggt gaaggagccg
     3601 aggctcacgg gtggcctccc accggcgtgc cggtacgtcg ggcacgctgc accgtgccgt
     3661 gtgcggtatg gggcggagcc gccaacctac gtgcccgttc ggattgtgcg caaaccgcgc
     3721 tccacatgcc actcacctcg acggagccat ccaggtcagg gcgcgtacga cccggtcggc
     3781 ggcgtcgcct cccgccggct cgtacaggtt cgccaggagc ttgaggacga tcttcatcag
     3841 cgccggacgg ggcagcccgt aggtcgtcgc gagcttcatc acgcgggggt cgccgatggc
     3901 cctcacgaac gcccggccga gggcgaagta cgagccgtag cggagctcca ccccggaccg
     3961 gtagcgccgc agcagggtcc ggtcgccgct cgcgagcacg gcgagccccg tctcggccgc
     4021 gagctggccg ctctccatcg cgtagtcgat gccctcgccg ttgaacgggt tgacgagccc
     4081 ggccgcgtcg ccgacgaaca tgaccccgcg gtgcaggggc gggtgccggt tggcggccat
     4141 cggcaggggc gccgaccgcg gcccgccgac ggcgtcggtg ggcgtgaagc cccagtcgcg
     4201 ccccaccgtc gggacccagt cggccagcag cctgcggtag ttggtgttgc ggaagtggtc
     4261 ggaggtgttc agcaggccga gaccgacgtt gacggtgccg tcggccatcg ggaacaccca
     4321 tccgtagccg ggcagcaggt cgtcgccccg ccacagctcg aggtaggagt cgagccaggg
     4381 gtcgtggtcc cgctcgctgg tgaagtactg ccggatggcg acgccgacgg gccggccggg
     4441 atcccggtgc agcccgaggg tctgggcgaa ccggctcgac gcgccgtcgg ccgccaccac
     4501 gacctcggcg tgcacgtcga ccgggtcctc gccctcgcgg cggacgaccg ccccgcggac
     4561 caggccgcgc tcgaagagcg gcaggacgac ctcggtcgac tcccagagcg tggccccggc
     4621 cttctcggcg tggcgggcga ggagctggtc gaagtcggtc cgcggccggg cgagcccgta
     4681 cgacgggtac cgggacagct ccggccaggg cagctcgagc acgaggccgc cgccgtgcac
     4741 cttgaggccg gcactgcgcg accaggtccg gtactcgtcg cgcaggccca tggtgtcgag
     4801 cgcggccacc gcgcggggcg tcagcccgtc gccgcagacc ttgtcgcggg gatagccggc
     4861 cttctccacc agcagcacgc gcgcaccggc acgggcgaag tggtaggcgg tcgccgcccc
     4921 acccggccct cccccgacga cgagcgcgtc gtactggctg gtcaccgtcg cctccggagc
     4981 cgcggcccgc ggccgcggcc gcgtgtcctt gtgaattgac gcacaaagat gctgctgaga
     5041 gtgtaggccg ggtcgcaccc cacggcaaac gacgctccct gtccgttcgg cacgcccctc
     5101 cctgtcccga tcggcgtgcg tgcacgcgaa gcatagttcc cccggcgccg aggagggggg
     5161 tttccgctcc tccgggtctg gggaccatgg tggtcatggt ggtccgcatg cgacagaagc
     5221 ggcgcccgcc cggcgccccg agagaggtcc gttcgatcga gcaggcccgc tgctgcgcga
     5281 ccgacggctg cgaggtccac ctgtcgcgct acaatccgac gccgcactgc ggtgtccacc
     5341 gcggctggga ccacgaagag gtgcaccgca agccccgcca cgccgggtga acggaggcac
     5401 gggtcaggcg gcggcgcccc agtgcagcgc gacgatgccg ccggtcaggt tgcgccagcg
     5461 gacccgctcg aacccggcct cccggaccca ccgggccagc ccttcctggt cgggccaggc
     5521 cccgatcgag cgggcgaggt actcgtatgc ctccgcgtcg ctcgagaggc ggcgcgccgc
     5581 gagcggcatg agccgggtca ggtagcgccc gtagacttgg tcgagcacgg ggacgacagg
     5641 gtgggagaac tcgcagacga ccaggcgccc cccggggcgc agcacgcggc ggaactcgag
     5701 caggccctgg cccggctcgg gcaggttgcg cagcccgaac gcgatcgtga ggacgtcggc
     5761 ggaggcgtcg gcgaggggca gccgcagcgc gtcggcgacc gcgaaccgga cgcgacggat
     5821 gtccgcccgg ccatcccccc ctcctcgccc ccccgggcgc ggcaggcccc gtcggaccat
     5881 ctcccaggag aagtcgaggc cgagcacgac ggcgcctggt gccagcccgg ccagctcccg
     5941 ggtcagcgca ccggtcccgc aggcgacgtc gaccgcggtc tcccccgcct ccaggccggt
     6001 cgacagggca gccgcccgcc gccacgcccg atcctggccg agcgagaaca cggtgttgac
     6061 aaggtcgtac ctgggcgcca cccggtcgaa catccgccgc acccccgccg aggtgcgctc
     6121 ggccgccggt gacgcgctca tggggcaggc tcctcgggtc ggcggcaggg cggccattct
     6181 cccatcccga gcgccccgtg acgcccagcg tcaccggacc gcggcggagg tacgccgctc
     6241 ggaagcccag aacgacgccc tccaggtcac ctcggccagc ctcgcctggc ccttcaggtc
     6301 ggggtggaag tagtcgagcg ccgagacctc gtcggcggtg aacgggtacc tgtaggtggc
     6361 gaggccgtcg aagcggcacg cggaccagcc ggtgcaggcg tcggcgagga cgccgttgta
     6421 ggcgatctcc cgctcggcga ccgcctggcg ttcctcgggt ccgttcgacc gggcgagcag
     6481 cgccgggcag atgtgggcca cccgccacac gaagcgcgcc ctgggatcgc cgtggaggac
     6541 ctcccagagc cggtggaggt cggggatgct gctcacgaag acgtgggcgc cggcaggcag
     6601 ccccgcgcgc aggaccgcga gcgtctgccg gaaccgctcg gcgaacaccg gcaccggcgt
     6661 catcctgtcc ggcgacgggg cgcagaggtc gttcgcgccg agcagcacgg tgacgtaccc
     6721 ggcccggcgg gcgaccgcct cgcccgcctg cctgggcccg tcggccacct tggccccgct
     6781 caccgcgagg tcccagtgcc cggcgacgtc cggccggagc gccctgatgc gctcgtagtg
     6841 gctcgcgatg ccgtcgaagg ggaaccagcc ggtcgaccag gaatggctcg ggtggtcgcc
     6901 gtaccagcag caggcgtcga acgcctggct gatcgagtcg ccgatggcgg cgaccgagcc
     6961 gggcggcggc ggggcggcgg gggcggccgc gtcggccgcc gggaccgcgg cggacaccag
     7021 cagggcgagg gcgagcacga cgcccaggga actccgccgc atgcgatccc tccatcaggg
     7081 cagacacaga cggacggcta cagcatcgca aaccggtgct ccctttccta tccctcgctc
     7141 cgaaccagcg tgtcgcgaga gggcccggcg gcattcagag cttcgggccg cgctggaagg
     7201 gcgtggacag cacgaccgcg gttcgggtgc gggcgacccc gtcgacgtcg cggaggcgct
     7261 ggagcacctc tgacagctcg gccacggacc gcgtgcggac caccagcacg tggctctcct
     7321 caccggcgac ggtgtacgcg gcctcgatct gctcgatgcc ggcgaagacc tccgacatgg
     7381 cggcggcgtt ggaggagtcg ctcggcagca cgctgaccag ggagaggacc gcggggccga
     7441 gcaggtccgg gtcgacctcg gcccggtagg cacggatcac cccgcgcgcc tcgagccggt
     7501 ggacccgctc gtggaccgtc ggggcggaca gcccgaccgc cttgccgatc tcggtgaagg
     7561 tcgccctcgc gttggcctgc agggcacgga ggatcgccag gtcggtgtcg tcgagggtcg
     7621 cccggacggc ctcggctccc tcgttgatcg gcactacttt gttcggcacc gtcggcccat
     7681 gattcgaaga atcttcctct ctgaccacgt tgcatccccg gaatattcag ctgcccttgg
     7741 cgtgttaccc gcagtgacga ccggagcacg accgaacatc caagcacgac atccaagcac
     7801 gacggagtgt gatccgcatg atgctgcacg tctcgaccgc cctggcggtc gcccaccagg
     7861 aggacctacg ggcccaggcc cgccagga
//
LOCUS       DASPYY010000084         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1277726, whole genome shotgun sequence.
ACCESSION   DASPYY010000084 DASPYY010000000
VERSION     DASPYY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277726"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VEP73_01185"
     CDS             complement(<1..344)
                     /locus_tag="VEP73_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018486930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYT25086.1"
                     /translation="MPIEIGQEAPGFALKDQNGEVRRLSDYRGDKNVVVVFYPLAFSG
                     ICTAELCQLRDDLPAFHNDDVATLAVSIDSPYVLRAFADRQGYEFPLLSDFWPHGQVA
                     KAYGVFNEERGFA"
     gene            complement(720..1469)
                     /locus_tag="VEP73_01190"
     CDS             complement(720..1469)
                     /locus_tag="VEP73_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYT25087.1"
                     /translation="MTGDVFEALAAARRAGRPVVMGTTVRTEGDPPSAPGNRALFGSG
                     GELLAGTIGCNGLDRRTGGDGRGLLEAGKRSGLRRYTSFEGEPGEGVVEVFLEAFAGQ
                     ARLLVGGGGPVAEALAAIGGIVDLTVRAELPGSVAFAAAADDLGPGDAVVFVDHDDPD
                     LVPAVTRVLQGQAGYVGVMGSRRHTPGFVAELRSAGLDLARLHSPTGLDIGARVPPEI
                     ALSILSEVLAATRGRPGDSLDLVGRPALPRN"
     gene            complement(1466..>2747)
                     /locus_tag="VEP73_01195"
     CDS             complement(1466..>2747)
                     /locus_tag="VEP73_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYT25088.1"
                     /translation="GTTAIFPSDEQTRRFLRRQRREDQWAELGPDPDAKYDEEVEVDL
                     SSLEPLIAKPSQPDNVVPVEEVAGTEVFQVCFGSSVNSWYEDLAIPAAVLRGHHLPPT
                     TAGTVSPGSRQILTTITTSGVLEDLERAGARILEPACGPCVGIGQAPPSGKASMRTFN
                     RNFKGRSGTVDDQVYLASPATAAATARNGRITDPRTLGDPPVVDDPDPVVDDFMILDP
                     SPPEERERVEIIRGPNIKTPPIPPPLPEDMSGRVLIVLPDNVSTGSMAPDGAIVMADR
                     SNVAAIAEYTFMKEDPEFVRRAKEWGGGFIVGGDNYGQGSSREHAALAPLQLGIRAVL
                     AQGFARIHRRNLIAQGLVPLLIDAEVHDRLEVGDRLSVRGLGAAVTAGAQEVDVEVEG
                     KDGFRAGLDLSPREREVVAVGGVVNQVREEAGTG"
BASE COUNT          348 a          978 c         1043 g          378 t
ORIGIN      
        1 gcgaagccgc gctcctcgtt gaagaccccg taggccttgg ccacctggcc gtggggccag
       61 aagtcgctga ggagggggaa ctcgtagccc tgcctgtccg cgaaggcgcg caggacgtag
      121 ggcgagtcga tcgacaccgc caacgtggcc acgtcgtcgt tgtggaaggc cggcaggtcg
      181 tcccggagct ggcagagctc ggcggtgcag atgcccgaga aggcgagcgg gtagaagacc
      241 acgacgacgt tcttgtcgcc gcggtagtcg gagagccggc gcacctcgcc gttctggtcc
      301 ttgagggcga agccgggcgc ctcctggccg atctcgatag gcatggtcgg gtcctttcgt
      361 acggtgctgc cggtcggcgg catccggccg gaaccgtgag tctacgtact ggcttggtac
      421 ccgaggggga cgaatctggg gattgaccta agcaacacgg tctagttaga gttcggcgtg
      481 tcggcacggt ggcctgcctt gtacccccgt gccgacgaac ggtaggtcgg gcgtcttcgc
      541 gccggaccag gcgccctggt ctcctcccgg gactggggcg cgacccgtcg gggccggttt
      601 cgtctggccg cggcggggct gggcccgctc cccggatcgg gcccagaggg tgccgttcgg
      661 ggatggagag ccccgagagg gtcgagggtg ccgcgcactc aaggcctgct cggggctctt
      721 cagttccgcg ggagggccgg acggccgacc aggtcgaggc tgtcgccggg ccgcccgcgg
      781 gtcgcggcca gcacctccga gaggatcgac agggcgatct ccgggggcac ccgcgcgccg
      841 atgtcgagcc cggtcgggct gtgcagccgc gcgaggtcga gcccggccga gcgcagctcg
      901 gcgacgaacc cgggcgtgtg gcggcggctg cccatgaccc cgacgtaccc ggcctggccc
      961 tggaggacgc gggtgaccgc gggcacgagg tcggggtcgt catggtcgac gaagacgacc
     1021 gcgtcgccgg ggccgaggtc gtcggcggcg gcggcgaacg cgacggagcc gggcagctcg
     1081 gcccggacgg tgaggtcgac gatcccgccg atggcggcga gggcctcggc caccggcccg
     1141 ccgccgccga ccagcagccg ggcctgcccg gcgaacgcct ccaggaagac ctcgacgacg
     1201 ccctcgcccg gctcgccctc gaagctcgtg tagcggcgca gcccggaccg cttccccgcc
     1261 tcgagcaggc cgcggccgtc gccgccggtc ctgcggtcga gcccgttgca gccgatcgtc
     1321 ccggccagga gctccccgcc cgaaccgaac agggcgcggt tgcctggcgc cgagggcggg
     1381 tcgccctcgg tccggacggt ggtgcccatg accaccggcc gcccggcccg ccgggcggcg
     1441 gcgagggcct cgaacacgtc gccggtcatc cggtgcctgc ctcctccctg acctggttga
     1501 ccacgccgcc gacggcgacc acctcgcgct cgcggggcga caggtcgagc ccggcccgga
     1561 acccgtcctt gccctcgacc tcgacgtcga cctcctgggc gccagcggtc acggccgcgc
     1621 cgagcccgcg gaccgagagc cggtccccca cctcgagccg gtcgtggacc tccgcgtcga
     1681 tgaggagcgg gacgaggccc tgcgcgatca gattgcgccg gtggatgcgc gcgaacccct
     1741 gcgcgaggac ggcgcggatg ccgagctgca ggggcgcgag ggccgcgtgc tcgcggctcg
     1801 agccctggcc gtagttgtcc ccgccgacga tgaagccgcc cccccactcc ttggcgcgcc
     1861 gcacgaactc cgggtcctcc ttcatgaagg tgtactcggc gatcgcggcg acgttggacc
     1921 ggtccgccat gacgatcgcc ccgtcgggcg ccatcgaccc ggtcgagacg ttgtcgggca
     1981 ggacgatcag cacccgcccg ctcatgtctt cgggcagcgg cggcgggatc ggcggcgtct
     2041 tgatgttcgg gccgcggatg atctcgacgc gctcccgctc ctcgggcggg gacgggtcga
     2101 ggatcatgaa gtcgtcgacc accgggtcgg ggtcgtcgac caccggcggg tcgcccagcg
     2161 tgcgagggtc ggtgatgcgg ccgttgcgcg cggtggccgc cgccgtcgcc ggcgaggcca
     2221 ggtagacctg gtcgtccacc gtgccggagc gccccttgaa gttccggttg aaggtccgca
     2281 tgctcgcctt gcccgacggg ggcgcctggc cgatgccgac gcacgggccg caggccggct
     2341 cgaggatgcg cgccccggcg cgctccaggt cctcgagcac gccggaggtc gtgatcgtgg
     2401 tcaggatctg cctcgagccc ggcgagaccg tgccggccgt ggtcgggggc aggtggtggc
     2461 cccgcaggac cgcggcgggg atggcgaggt cctcgtacca ggagttgacg ctcgacccga
     2521 agcagacctg gaacacctcg gtgcccgcga cctcctccac cggcacgacg ttgtcgggct
     2581 gggacggctt ggcgatcagg ggctcgaggc tcgagaggtc gacctcgacc tcctcgtcgt
     2641 acttcgcgtc cgggtcgggg ccgagctcgg cccactggtc ctcccgccgc tggcgccgca
     2701 ggaaccgcct ggtctgctcg tcggacggga agatcgcggt cgtcccg
//
LOCUS       DASPYY010000085         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_294378, whole genome shotgun sequence.
ACCESSION   DASPYY010000085 DASPYY010000000
VERSION     DASPYY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294378"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEP73_01200"
     CDS             <1..249
                     /locus_tag="VEP73_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25089.1"
                     /translation="VYPFVVPLRPVPGSLMERCRPPAPDDMDAVYRQVAPYLVARGVA
                     SWDVRAGCARCQACSAISILERDLASDQRTHLPILDVR"
     gene            203..730
                     /locus_tag="VEP73_01205"
     CDS             203..730
                     /locus_tag="VEP73_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HYT25090.1"
                     /translation="MPATSAPISPFSTCADLDAAVGAEPALGCRLVATPAERALHLRI
                     RAEVFVGEQGLFSVTDRDAHDDDPDTLHVLGLCGPVAAGAVRLYPLDEPGLWKGDRLA
                     VLPGLRKHGLGAPLVRFAVRTAGERGGREMLAQVQLANVGFFERLGWGRLGEPGDYCG
                     RPHQRMRIGLRPPRT"
     gene            complement(747..1715)
                     /locus_tag="VEP73_01210"
     CDS             complement(747..1715)
                     /locus_tag="VEP73_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HYT25091.1"
                     /translation="MEAAALLEEVVNEVRAHAGLRGKSPLGLVTEVLGPTDWRSGPGD
                     DGAVVADGASTLVVGGEALLDRFVAADPFAAGVAAVLTNVNDLAAMGAWPLAIVDTVV
                     ASEAVARRALQGMRHASELYRVPVVGGHLTIRGGPPGISAFGLGRVARVLSVRAAAPG
                     QTLLVACCLEGTMRDDFPFFASLPRRGAAVADDVRLLPRLAESGACVAAKDVSMAGLL
                     GSLAMLLEYGRLGAAVDLDALPRPAGVPLARWLVAFPAFAFLLCAPPGQADACRAPFL
                     DRGLACEPVAVLDGSGALRARLGGAEALVLDLRRETVTGLGNRVPG"
     gene            1783..2574
                     /locus_tag="VEP73_01215"
     CDS             1783..2574
                     /locus_tag="VEP73_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HYT25092.1"
                     /translation="MASSGFLDLPPRSSKPRRAGVTHVLDKGLPVAGMEPLLAILADH
                     VDLWKLGWGTAYLDPGAGDKVALLARHEVRACVGGTLLEVAWAQGKAEACLAWAGGLG
                     FPCVEVSNGAVGMPLAEKRRLIAQATEGFTVLAEVGAKDPGVPVSPSRWAAEMLGDLE
                     AGASWAVAEGRESGTVGLYRPDGSVRDDVVDAVVGAVGLRTLIFEAPARQQQAWFIRR
                     FGPDVSLGNVPAPEVLGLEALRLGLRADTIELARSRSAAVTVAGP"
     gene            2571..3455
                     /locus_tag="VEP73_01220"
     CDS             2571..3455
                     /locus_tag="VEP73_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25093.1"
                     /translation="MSNLVPGRYRDVSVATVERGLSAEDLERHFLGREAYRRTRYVVA
                     RRGPEVALVEVTKASELPLLSPIVGVRVLAPPGECAVVDAPEVDTGVPSQLARAARAL
                     APGARCVVVQGRYRHVSFILDPDPIRVRVVEVVPPEPPKLLDQARRVLEVAEDLPPIE
                     LLGDLTDLTELAGRRPARRYLLPCRGSGVRIDGAETSFLDERPPLREWVLIGCARSRE
                     LYGWFYGAEAEAVDMCPRRLAGPAGGTPVLTKCCLLEEHVEREGARVTVPWGASLELV
                     REGLARVAAMAEPAWPPA"
     gene            3440..4816
                     /locus_tag="VEP73_01225"
     CDS             3440..4816
                     /locus_tag="VEP73_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HYT25094.1"
                     /translation="MAARLSDSELYAHLWGTGELRAVFEERARLQSWLDVLAALAEAQ
                     AELGIIPAEAATAVARSASVDRLDLAFVAAETRRTSHSTLGIIRGLERVLPPEAREYV
                     YYGATVQDVTDTWTALVVRRVGGVVWRDLRRIRAHLVDLAERHRDTVMAGRTHGQPGA
                     AITFGFKAASWADEVHRHVARLRQGAPRWLVGQLAGAVGTVAFFGDRGLALRARFCAR
                     LGLADPGISWLTSRDRLAEFGHLLAMVTATLARIGTEVYELQRPEIGELREPAGHDAV
                     GSITMPHKRNPEGAEHLATLARLVRAQAGVLTESMVAEHERDGRAWKAEWLAFPEACL
                     LAGAALELAERLLAGLEVGTEAMAANLAAARGYLASEQVLAMLAPTVGKHRAQALLQE
                     ALRAGSARGQPLGDALLASAALRPYLDPGALGPLLADPDAGSAAAMADEVVRRIRADE
                     AAEAGGWP"
BASE COUNT          556 a         1872 c         1827 g          576 t
ORIGIN      
        1 gtgtacccgt tcgtcgtgcc gctgcggccc gtgccgggca gcctcatgga gcgctgccgg
       61 cccccggccc ccgacgacat ggacgccgtc taccgccagg tcgctcccta cctggtggcc
      121 cggggggtgg cgtcgtggga cgtccgcgcc ggctgcgccc gctgccaggc gtgctcggcc
      181 atctccatcc tggagcgcga ccttgccagc gaccagcgca cccatctccc cattctcgac
      241 gtgcgctgac ctcgacgcgg ccgtcggcgc ggagccggcg ctcggctgcc gcctggtggc
      301 cacgccggcc gagcgcgcgc tgcacctgcg catccgggcg gaggtcttcg tcggggagca
      361 gggcctgttc tccgtgaccg accgggacgc acacgacgac gacccggaca cgctgcacgt
      421 gctggggctg tgcggcccgg tggccgccgg cgccgtgcgc ctgtacccgc tggacgagcc
      481 cgggctgtgg aagggcgacc gcctggccgt gctgcccggg ctccgcaagc acggcctcgg
      541 ggcgccgctg gtgcggttcg cggtgcgcac ggccggcgag cggggcggcc gggagatgct
      601 cgcccaggtg cagctcgcca acgtcggctt cttcgagcgg ctcgggtggg gccggctcgg
      661 cgagccgggc gactactgcg gccggcccca ccagcgcatg cgcatcggcc tgcgaccgcc
      721 ccgcacctga ccgggccggc ggacgctcag cccggcaccc ggttcccgag gcccgtcacc
      781 gtctcgcggc gcaggtccag gacgagcgcc tccgccccgc cgaggcgggc ccgcagcgct
      841 cccgagccgt cgaggacggc gaccggctcg caggcgaggc cgcggtcgag gaacggggcc
      901 cggcacgcgt ccgcctgccc ggggggcgcg cagagcagga acgcgaacgc cgggaaggcg
      961 acgagccagc gggcgagcgg caccccggcc gggcggggca gcgcgtccag gtcgacggcg
     1021 gcccccagcc ggccgtactc gagcagcatg gcgagcgagc cgagcagccc cgccatgctg
     1081 acgtccttgg ccgccacaca ggcgcccgac tcggccaggc ggggcagcag gcggacgtcg
     1141 tcggcgacgg ccgcgccgcg ccgcggcagc gacgcgaaga acgggaagtc gtcccgcatg
     1201 gtcccttcca ggcagcaggc gaccagcaag gtctgccccg gggcggcggc gcgcaccgac
     1261 agcacgcgcg ccacccggcc gaggccgaac gccgagatcc ccggcgggcc gccccggatc
     1321 gtgaggtgac cgcccaccac gggcacccgg tacagctcgg acgcgtgccg catgccctgc
     1381 agggcgcgcc gggcgaccgc ctcggaggcg accacggtgt ccacgatggc cagcggccac
     1441 gcccccatgg cggccaggtc gttgacgttg gtgagcaccg cggcgacccc ggcggcgaac
     1501 gggtccgccg ccacgaagcg gtccaggagc gcctcgcctc cgaccacgag cgtcgaggcg
     1561 ccgtcggcga cgacggcccc gtcgtcgccc ggcccgctgc gccagtccgt gggcccgagc
     1621 acctcggtca ccaggccgag cggggacttg ccgcgcaggc cggcgtgggc cctgacctcg
     1681 ttcacgacct cctcgagcag cgctgccgcc tccataccgt ccctcccggc gcgaccggac
     1741 gcctcttgag gaagatcaca tgcccgttta ctatacgaaa cgatggccag cagcggcttc
     1801 ctcgacctcc cgccccgcag ttccaagcct cgacgggcgg gggtcaccca cgtgctcgac
     1861 aagggcctcc ccgtggccgg gatggagccg ctcctggcga tcctcgccga ccacgtcgac
     1921 ctctggaagc tcggctgggg gaccgcgtac ctcgacccgg gcgccggcga caaggtcgcc
     1981 ctgctggccc ggcacgaggt acgcgcctgc gtcggcggga ccctcctcga ggtcgcctgg
     2041 gcccagggca aggccgaggc ctgcctggcc tgggccggcg ggctcggctt cccctgcgtg
     2101 gaggtgtcga acggcgcggt cggcatgccg ctggccgaga agcgacggct gatcgcccag
     2161 gccaccgagg ggttcaccgt cctggccgag gtcggcgcga aggacccggg cgtcccggtc
     2221 tcgccctccc gctgggccgc cgagatgctg ggcgacctcg aggccggggc gtcctgggcg
     2281 gtggccgagg gccgcgagag tggcaccgtg ggcctgtacc ggccggacgg ttcggtacgg
     2341 gacgacgtgg tcgacgccgt ggtgggcgcc gtcggcctgc gcacgttgat cttcgaggcc
     2401 ccggcaaggc agcagcaggc ctggttcatc cgccgcttcg gccccgacgt gagcctcggc
     2461 aacgtgccgg ccccggaggt gctcggcctg gaggcgctgc gcctcgggct gcgggccgac
     2521 acgatcgagc tggcgcgctc gcgcagcgcg gccgtgaccg tggcggggcc gtgagcaacc
     2581 tcgtcccggg ccgctaccgc gacgtgtcgg tcgccacggt cgagcgcggc ctcagcgccg
     2641 aggacctcga gcgccacttc ctcggccgcg aggcctaccg ccggacccgc tacgtcgtcg
     2701 cccgccgggg tcccgaggtg gccctcgtcg aggtgaccaa ggcgtccgag ctcccgctgc
     2761 tctcgccgat cgtgggcgtc cgggtcctcg cgccgcccgg cgagtgcgcc gtggtcgacg
     2821 ccccggaggt cgacaccggc gtcccctccc agctcgcgcg ggcagcccgc gcgctggccc
     2881 ctggcgctcg ctgcgtggtc gtgcagggac gctaccgcca cgtcagcttc atcctcgacc
     2941 ccgacccgat ccgggtccgc gtggtcgagg tcgtcccgcc ggagcctccg aagctgctgg
     3001 accaggcccg ccgggtgctg gaggtggccg aggacctccc gccgatcgag ctgctcggtg
     3061 acctcaccga cctcaccgag ctggccgggc ggcggcccgc ccggcgctac ctgctgccgt
     3121 gccggggctc gggcgtccgc atcgacggcg ccgagaccag cttcctcgac gagcggccgc
     3181 ccctgcggga gtgggtgctc atcggctgcg cccgctcccg cgagctgtac ggatggttct
     3241 acggagcgga ggccgaggcg gtggacatgt gccccaggcg gctggccgga ccggcgggcg
     3301 gcacgccggt gctcaccaag tgctgcctgc tcgaggagca cgtcgagcgc gagggcgccc
     3361 gcgtgaccgt cccctggggc gcctcgctgg agctcgtgcg cgaggggctg gcccgcgtcg
     3421 cggccatggc ggagccggca tggccgcccg cctgagcgac tccgagctgt acgcccacct
     3481 ctgggggacc ggcgagctgc gcgcggtgtt cgaggagcgc gcccgcctgc agtcctggct
     3541 cgacgtcctg gcggccctgg ccgaggccca ggccgagctc ggcatcatcc cggccgaggc
     3601 ggccacggcc gtggcccgct cggcgtcggt cgaccggctc gacctggcgt tcgtggccgc
     3661 cgagaccagg cggacctcgc actccacgct cgggatcatc cgcggcctcg agcgcgtgct
     3721 cccacccgag gcgcgcgagt acgtctacta cggcgccacg gtccaggacg tcaccgacac
     3781 ctggacggcg ctcgtggtgc gccgcgtcgg cggggtcgtc tggcgcgacc tgcgccggat
     3841 ccgggcgcac ctggtcgacc tggcggagcg ccaccgtgac acggtcatgg ccgggcgcac
     3901 gcacgggcag ccgggcgccg cgatcacctt cgggttcaag gccgcctcct gggccgacga
     3961 ggtccaccgg cacgtcgccc gcctgcgcca gggcgcgccg cgctggctgg tcggccagct
     4021 cgccggcgcg gtcgggacgg tggcgttctt cggggaccgg gggctcgccc tgcgggcccg
     4081 cttctgcgcc cgcctggggc tcgccgaccc gggcatctcc tggctcacct cccgcgaccg
     4141 cctggccgag ttcggccacc tcctggccat ggtcacggcc accctggccc ggatcgggac
     4201 cgaggtgtac gagctgcagc ggcccgagat cggcgagctg cgcgagccgg caggccacga
     4261 cgcggtcggg agcatcacca tgccccacaa gcgcaacccg gagggcgccg agcatctcgc
     4321 caccctcgcc cgcctggtca gggcccaggc gggcgtgctg accgagagca tggtggccga
     4381 gcacgagcgc gacgggcggg cctggaaggc ggagtggctg gcgttccccg aggcctgcct
     4441 gctggcgggc gccgccctcg agctcgccga gcggctgctg gccgggctcg aggtgggcac
     4501 cgaggcgatg gcggccaacc tggccgccgc gcgcggctac ctggcgtccg agcaggtcct
     4561 cgccatgctg gcacccacgg tcggcaagca ccgggcccag gccctgctcc aggaggcgct
     4621 gcgggcgggg tcggcccggg gccagccgct cggggacgcc ctgctggcga gcgcggcgct
     4681 gcggccctac ctcgaccctg gcgccctggg accgctgctg gccgaccccg acgccggctc
     4741 ggccgcggcc atggccgacg aggtcgtccg gcggatccgc gccgacgagg ctgccgaggc
     4801 gggaggctgg ccgtgaaccc gtccggccgc t
//
LOCUS       DASPYY010000086         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1278237, whole genome shotgun sequence.
ACCESSION   DASPYY010000086 DASPYY010000000
VERSION     DASPYY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278237"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..>1534
                     /gene="alaS"
                     /locus_tag="VEP73_01230"
     CDS             165..>1534
                     /gene="alaS"
                     /locus_tag="VEP73_01230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYT25095.1"
                     /translation="MNSIEIRRRFTDFFVARGHRLVPSSPLIPNDPTLLLTNAGMNQF
                     KPYFLGEAVPEFTRATSVQKCARTPDIELVGHTTRHVTFFEMLGNFSFGDYFKAQAVR
                     YAYELATGHYGLDPDRLWATVYLDDDEAVGYWREVGIPLERIQRLGKEDNFWSMGVAG
                     PCGPCSEIYYDRGPAYGREGGPAVDPERYLEFWNLVFMQNIRDDDYDIVGELPKKNID
                     TGLGFERLAVILQGVGNVFETDVLAPIVQQVQDVTRRRLGESDRTDVSLKVVADHART
                     SSFLIADGVLPSNEARGYVLRRLLRRAVRHVRLLGADDLALPAVTSRVVDTLGEAWPE
                     LAAQRSLLAQVVASEEESFARTLRQGSALLDSAIATTRERRGTVLSGETAFRLHDTYG
                     FPLELTLEAATDAGLSVDRDQFAVLMEDQRRRAREARKELAGAQRKVEAYRDLAARYG
                     PTSFVG"
BASE COUNT          222 a          565 c          534 g          213 t
ORIGIN      
        1 ccaggctggc cggcgcgctc gaggagggcc gggccgccat ggcggcccgc gaggcggagc
       61 tgcgagctca ccgcgccgga ccggggaacg gggcggcctg acgtcccggc ctggtccggc
      121 ggggggtccc aggcttccgt cgagcgtcac gaggtcaaca tcccatgaac tccatcgaga
      181 tccgtcggcg gttcaccgac ttcttcgtgg ctcgggggca ccggctggtg ccctcgagcc
      241 cgctcatccc gaacgacccg accctgctgt tgaccaacgc ggggatgaac cagttcaagc
      301 cctacttcct cggcgaggcg gtgcccgagt tcacccgggc gaccagcgtg cagaagtgcg
      361 cccgcacgcc cgacatcgag ctggtcggcc acaccaccag gcacgtcacg ttcttcgaga
      421 tgctcggcaa cttctcgttc ggcgactact tcaaggccca ggccgtccgc tacgcctacg
      481 agctggccac cgggcactac ggcctcgacc ccgaccgcct gtgggccacc gtctacctcg
      541 acgacgacga ggccgtcggc tactggcggg aggtcggcat cccgctcgag cgcatccagc
      601 ggctcggcaa ggaggacaac ttctggtcga tgggcgtggc cgggccctgc ggcccctgct
      661 cggagatcta ctacgaccgc ggcccggcct acggcaggga ggggggcccg gcggtcgacc
      721 cggagcgcta cctcgagttc tggaatctcg tgttcatgca gaacatccgt gacgacgact
      781 acgacatcgt cggcgagctg cccaagaaga acatcgacac cggcctcggg ttcgagcggc
      841 tcgcggtcat cctgcagggc gtcggcaacg tgttcgagac cgacgtgctc gcgcccatcg
      901 tgcagcaggt ccaggacgtc accaggcgcc ggctggggga gtcggaccgg accgacgtgt
      961 cgctcaaggt cgtcgccgac cacgcccgca cctcgagctt cctgatcgcc gacggggtgc
     1021 tgccgtccaa cgaggcccgc gggtacgtgc tgcgccggct gctgcgccgc gcggtgcgcc
     1081 acgtccgcct gctcggcgcg gacgacctcg ccctgcccgc ggtgacgagc cgggtggtcg
     1141 acaccctcgg cgaggcgtgg ccggagctcg ccgcccagcg gtcgctcctc gcccaggtgg
     1201 tggcgagcga ggaggagtcg ttcgcccgca ccctgcgcca ggggtcggcc ctgctcgact
     1261 cggccatcgc caccaccagg gagcggcgcg gcacggtcct ttccggcgag accgcgttcc
     1321 ggctccacga cacctacggg ttccccctgg agctcaccct ggaggcggcc accgacgcgg
     1381 ggctctcggt ggaccgcgac cagttcgcgg tcctcatgga ggaccagcgg cgccgcgcca
     1441 gggaggcccg caaggagctc gcgggcgccc agcgcaaggt cgaggcatac cgtgacctcg
     1501 ccgcgcggta cgggccgacc agcttcgtcg gcta
//
LOCUS       DASPYY010000087         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2402246, whole genome shotgun sequence.
ACCESSION   DASPYY010000087 DASPYY010000000
VERSION     DASPYY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402246"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEP73_01235"
     CDS             <1..318
                     /locus_tag="VEP73_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25096.1"
                     /translation="AYVNPVVAFILGALLLGEAISPRTVVAAVVIVAAVALIVTARSR
                     SGPEAQTEDAHPEAESDADADHAPDTTAGEPSPVTDRPAAFGGPPVMPRPGPSARPDA
                     PGA"
     gene            complement(269..>1840)
                     /locus_tag="VEP73_01240"
     CDS             complement(269..>1840)
                     /locus_tag="VEP73_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYT25097.1"
                     /translation="RLPADGRGPLQLTADVVVVGSGAGGGVMAAELARAGRSVVVLEA
                     GSFVPEPEMPTDELAAYDRLYLDHGLTSTWDGAISILAGSVVGGGTTVNWMTSIPVQP
                     AVRAEWAREHGLDGVDGAELEADLAVIERELAVQGPPNMPAKDAAILRGASALGWEAA
                     ETRRNGVGCGDCGSCPFGCRLGAKQGGLRVHLAEAWRCGARIVPEATVTRVLLGPAPD
                     ATGPHATGPYAAGVEAALADGRRLNVVASQTVIAAGALRTPVILERSGLQHPAIGRHL
                     HLHPVTVIAALLADPVAMWSGTTQAARSLEFLDPRTPGGSGFVIESAPAHPGLIGLAF
                     PWQSAGAFAENMGRILHVAPLVAIGRDLGAGRVRARPAGGARIDYRLAATDIATLRRG
                     LVELARLGRAAGAEELVALGSPAAWFGRDRPMREAGAAAFEAYLARLAAFDFAPNRAM
                     VFSAHQMGTARMGARPADHVCDERGRVRTDRGRVIRGLYVADASLFPTAIGVNPMITV
                     MLLARRVSRTVLAEA"
BASE COUNT          254 a          719 c          665 g          202 t
ORIGIN      
        1 gcctacgtca acccggtcgt cgcgttcatc ctgggcgcgc tcctgctcgg cgaggccatc
       61 tcgccgcgga ccgtcgtcgc ggccgtggtg atcgtggccg ccgtggccct catcgtcacg
      121 gcccgcagcc gctcggggcc cgaggcgcag acggaggatg cacatcccga agccgagtcc
      181 gatgccgacg cggaccacgc gcccgacacc accgccggcg agccctcccc ggtgaccgac
      241 cggccggccg cgttcggcgg gccgccggtc atgcctcggc caggaccgtc cgcgagaccc
      301 gacgcgccag gagcatgacc gtgatcatcg ggttgacgcc gatcgcggtc gggaacagcg
      361 aggcgtcggc cacgtagagg ccccgaatga cgcggccgcg gtccgtccgg acgcgaccgc
      421 gctcgtcaca cacgtggtcg gcgggccgag cacccatgcg cgccgtgccc atctggtgcg
      481 cggagaagac catcgcccgg ttcggggcga agtcgaacgc ggcgagccga gccaggtacg
      541 cctcgaaggc ggccgcgccg gcctcgcgca tcggtcggtc gcggccgaac caggccgcgg
      601 gagagccgag cgccacgagc tcctcggctc cggccgcgcg gcccagccgc gccagctcga
      661 ccaggccgcg ccggagcgtg gcgatgtccg tggccgccag ccggtagtcg atccgggctc
      721 ctccggcggg acgcgcccga acccgaccgg cgcctagatc gcggccgatc gcgacgagcg
      781 gcgccacgtg aaggatgcgg cccatgttct cggcgaaagc ccccgcggat tgccacggaa
      841 aggcgaggcc gatcaggcct ggatgtgccg gcgccgactc gatgacgaat ccggagcctc
      901 ccggcgtgcg cgggtcgagg aactccaggg agcgcgccgc ctgcgtcgtg cccgaccaca
      961 tcgcgaccgg gtcggccaac agtgcggcga tgacggtgac cgggtggaga tgcaggtggc
     1021 ggccgatcgc cgggtgctgc aggccgctcc gctccaggat caccggcgtc cgcagcgcgc
     1081 cggcggcgat cacggtctgc gacgcgacga cattcagccg gcgcccgtcc gcgagcgccg
     1141 cctccacgcc ggccgcgtac ggcccggtcg cgtgcggccc cgtggcgtcc ggcgccggac
     1201 ccagcagcac ccgcgtcacg gtggcctcgg gcacgatccg ggcgccgcag cgccaggcct
     1261 ccgcgagatg gacgcgcagg ccgccctgct tcgccccgag ccggcagccg aacgggcagg
     1321 agccgcaatc gccgcagccg accccgttcc gccgcgtctc ggcggcctcc cagcccaggg
     1381 ccgacgctcc gcgcaggatc gcggcgtcct tggccggcat gttcggcggt ccctgcaccg
     1441 cgagctcccg ctcgatgacg gccagatcgg cctccagctc ggcaccgtcg acgccgtcca
     1501 ggccgtgctc acgtgcccac tcggcgcgaa ccgccggctg gaccgggatc gaggtcatcc
     1561 agttgacggt cgtgccgccg cccacgaccg aaccggcgag gatcgagatc gcgccatccc
     1621 aggtggacgt caggccatgg tccaggtaca gccgatcgta cgcggccagc tcgtcggtcg
     1681 gcatctccgg ctcagggacg aacgagcccg cctccaggac cacgaccgag cggccggccc
     1741 gggcgagctc ggcggccatc acgccgccgc cggcgccgga gcccacgacc acgacgtccg
     1801 ccgtgagctg gagcgggccg cgtccatcgg ccggcaggcg
//
LOCUS       DASPYY010000088         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_14531, whole genome shotgun sequence.
ACCESSION   DASPYY010000088 DASPYY010000000
VERSION     DASPYY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14531"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VEP73_01245"
     CDS             complement(<1..634)
                     /locus_tag="VEP73_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYT25098.1"
                     /translation="MGWVGGTGAALGVAGAAILAWTAAGRTPRAVDPGFDSFVAGWRR
                     AHRIPDGPREPGGRVTLAWLRVPWAVGVVLARRGVDPDRLTACALWLALAAGWTAALG
                     PGWAAGAGALTLLAGVADGVDGAVAVLERRTSAFGAVWDSTADRLADLAMVAGPVALV
                     ATRAPDPRPALAAGAAAAAAGLLSEYVRARAQATGVVAAWTLVTPGERPAR"
     gene            828..1838
                     /locus_tag="VEP73_01250"
     CDS             828..1838
                     /locus_tag="VEP73_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT25099.1"
                     /translation="MVVPARDEAPRIGGCVAALLAQDVAAAEVVVVDDGSRDATARLA
                     ARAGARVVPAPTLPAGAAGKAAACAAGAAAARAGEWLAFVDADVRLAPEALSRLVAAA
                     ARSGSAAASPLARPLAGSWWEELLLPDLGLQVAERLDPGALADPARPDAFLSGQCLLV
                     RRDAYEAVGGFAAVAGSLVEDVALARRLKAGGYGLAVRLAPGLAAVRMYERPGQLAEG
                     LARNLAEVWGGDARAVARQAGRALLAALPWAALAAPRLRRAGLPRAALLAAGLLHLAT
                     RAGGRLRAGADPRWALAYPVADAVLLAVYLDSVHRRLAGRPVAWKGRAYPAGRAGPRP
                     PP"
     gene            complement(1878..2351)
                     /locus_tag="VEP73_01255"
     CDS             complement(1878..2351)
                     /locus_tag="VEP73_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HYT25100.1"
                     /translation="MATRAEPFAVFVFGMRLNRLRGLPRLLWGLRVLRRVLDDLEGHE
                     RGFLAGRVYRSGRTLVAIQYWESFDALDAWARDHALPHRGPWQRYLREALRDPAVGLW
                     HETYLARPGTWEGVYVNMPPWGLGEAGEVVEMQATRGSARDRLRERRKRGDQPPP"
     gene            complement(2797..>3246)
                     /locus_tag="VEP73_01260"
     CDS             complement(2797..>3246)
                     /locus_tag="VEP73_01260"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT25101.1"
                     /translation="RPNCSYKGGEPGFVRVVDERTIAFPNYDGNGMFLSMGNLLRNPE
                     VGLLFIDFERGHRLRLNGTASIDEQDELLAGWPEARLVVRVRAREVFPNCPRYIHRHQ
                     LLQRSRFVPRAGCQTPVPAWKRAGWAADALPAGDPANDPRDREVLDR"
BASE COUNT          341 a         1312 c         1227 g          366 t
ORIGIN      
        1 cgcgggcggg ccgctcgcca ggcgtcacca gcgtccaggc cgccaccacg cccgtcgcct
       61 gcgcccgcgc ccggacgtac tcggagagca gcccggcggc ggcagccgcc gcgcccgccg
      121 cgagcgccgg ccgcgggtcc ggcgcccgcg tcgcgaccag tgcgaccggc cccgccacca
      181 tggcgaggtc ggccagccgg tcggcggtgg agtcccagac ggccccgaag gccgaggtgc
      241 gccgctccag caccgcgacc gccccgtcga ccccgtcggc caccccggcc aggagcgtga
      301 gcgcgcccgc gccagccgcc cagcccggcc cgagcgccgc ggtccagccc gcggccagcg
      361 ccagccagag cgcgcacgcg gtgagccggt ccgggtccac gccccgccgg gccaggacca
      421 cgccgacggc ccagggcacg cgcagccatg cgagcgtgac ccggccgccc ggctcgcggg
      481 gcccgtccgg gatgcggtgg gcgcggcgcc agcccgccac gaaggagtcg aaccccgggt
      541 cgaccgccct cggcgtgcga ccggcggccg tccacgcgag gatggcggcg ccggccacgc
      601 cgagcgccgc gccggtccca ccgacccagc ccaccgagag gacctcctcg cccactccgt
      661 cgcccactcc gtcacggccc cggcggcccg ttgccgcctg gccggcgctc ctgctcgccg
      721 ccgcgagcgt cggcgccgcg gccgacagcc tcgcacgcct ggcccgggcg gggcgacgcc
      781 gctaccggct gggcccgggc gacccggcgg cggccgcctc ggtcagcgtg gtcgtccccg
      841 cccgcgacga ggcgccgcgg atcggggggt gcgtcgccgc cctgctcgcc caggacgtcg
      901 cagcggccga ggtcgtggtc gtggacgacg gctcccggga cgcgaccgcc cggctcgcgg
      961 cccgggcggg cgcccgggtc gtcccggcgc cgaccttgcc ggcgggcgcc gccggcaagg
     1021 cggccgcctg cgcggccggg gcggcggcgg cccgggcggg ggagtggctc gcgttcgtcg
     1081 acgccgacgt ccgcctggcc ccggaggcgc tctcccggct cgtggccgct gccgcccgct
     1141 cggggtcggc ggccgcgagc ccgctcgccc ggccgctggc cggctcctgg tgggaggagc
     1201 tgctcctgcc cgacctcggc ctgcaggtcg ccgagcgcct cgatcccggt gccctggccg
     1261 acccggcccg ccccgacgcc ttcctgtccg gccagtgcct gctcgtccgg cgcgacgcct
     1321 acgaggccgt gggcgggttc gcagccgtgg ccggctcgct ggtcgaggac gtcgcactcg
     1381 ccaggcggct caaggccggg ggctacggcc tggccgtcag gctcgcgccc gggctcgccg
     1441 cggtgcgcat gtacgagcgg cccgggcagc tcgcggaggg gctcgcacgg aacctggccg
     1501 aggtgtgggg aggcgacgcc cgcgcggtgg ccaggcaggc cggccgcgcg ctcctcgccg
     1561 ccctgccatg ggccgcgctc gccgccccgc gcctgcggcg ggccggcctc cctcgggccg
     1621 cgctgctcgc cgccgggctg ctgcatctcg cgacgcgcgc cgggggccgg ctgcgcgccg
     1681 gcgccgatcc ccggtgggcg ctcgcctacc cggtcgccga cgccgtcctg ctcgccgtct
     1741 acctcgacag cgtccaccgc cgcctcgccg gccgcccggt cgcctggaag ggccgcgcct
     1801 acccggccgg ccgggcaggc ccccgcccac cgccatgacg gcccgccggc cgggagtgct
     1861 ccggctgacc gggcgcgtca cggcgggggc tggtcgcccc gcttgcgccg ctcgcggagg
     1921 cggtcgcggg ccgagcccct ggtcgcctgc atctcgacca cctcgccggc ctcgccaagg
     1981 ccccacggcg gcatgttgac gtacacgccc tcccaggtcc ccggacgggc caggtaggtt
     2041 tcgtgccaca ggccgacggc ggggtcgcgc agcgcctcgc gcaggtaccg ctgccatggt
     2101 ccgcggtgcg gcagggcgtg gtcgcgggcc caggcgtcga gcgcgtcgaa cgactcccag
     2161 tactggatcg cgacgagcgt ccgcccggac cggtagaccc ggccggccag gaaaccgcgc
     2221 tcgtggccct ccaggtcgtc gagcacgcgg cggagcaccc ggagccccca gaggagccgc
     2281 ggcaggccac gcaggcggtt gagccgcatg ccgaacacga acacggcgaa cggctcggcc
     2341 cgcgtggcca tccaccggcc ggtccgcacc ggctctgagc tgctgtttct cctcgtcagg
     2401 ctcactcgcc ccctgttgac gccgtagcac agcgctcgta cgccacatta cttggcgtgc
     2461 tgagagcgcg gcggccggcg gtgatcgcct cggcggcggc ggcgtgccgg tccagccggg
     2521 gccggcccct gctcctggtc ggtgatcggc accggccgtc cctactatca ggccgcgctc
     2581 gccacctgac gggtggccag gtgagcccct gcccagcagg ggctttcgca cttcctgggc
     2641 aagggagtca atggggcggt acgaccaagc cgacagtggg tggcagaggc agcgagcagg
     2701 agaacggtga gtggagctga ttctccggct gggcaggatc ggggtgccga tgatgcttcg
     2761 cccgcgccgc ggtcgcctgt tgcatccgcg tctgggttac cggtcgagca cctcccggtc
     2821 gcgtgggtcg ttggccgggt cgcccgccgg cagggcgtcg gcggcccacc cggcgcgctt
     2881 ccatgcgggc accggggtct ggcagccggc gcggggcacg aacctggacc gctgcagcag
     2941 ctggtggcgg tggatgtagc gagggcagtt cgggaacacc tcgcgggcgc gcacccgcac
     3001 caccagccgc gcctccggcc agccggccag cagctcgtcc tgctcgtcga tgctggccgt
     3061 gccgttcagg cgcaggcggt ggccccgctc gaagtcgatg aacagcagcc cgacctccgg
     3121 gttgcgcagg aggttgccca tcgacaggaa catgccgttg ccgtcgtagt tggggaacgc
     3181 gatggtccgc tcgtccacca cccggacgaa gcccggctcg ccacccttgt aggagcagtt
     3241 cggccg
//
LOCUS       DASPYY010000089         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_716662, whole genome shotgun sequence.
ACCESSION   DASPYY010000089 DASPYY010000000
VERSION     DASPYY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716662"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1694)
                     /locus_tag="VEP73_01265"
     CDS             complement(<1..>1694)
                     /locus_tag="VEP73_01265"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYT25102.1"
                     /translation="ALTVSGGGVVDTLSLIGVAPGTAFALSDDGRGGTEVHEVPPAPS
                     GLVLLSDSGTPGDNRTNVTVPLIGGSGEAGDRVRLFDGGADTGQSTIVGAGGGWVLAP
                     SLAAGVHLLTASESDGAGEVSLPSAGLSVTIDTSAPLTAVVSASFAFDGGAAPIGIAS
                     PSDSDDPADALGVTVTGLPGNGTLTLGDGVTPVTAGEPLTIGQLSGLLFTPAADRPAA
                     GGSFGYSVTDRAGNTAAGGATLTVVPSATVSFFDFLFAYGDGKDYYYGRAADNGTFGY
                     HVGQHISVGLGQYTVFDKEGLPAPVAAGSVFVSNYSHDGPGAASPVPLGFPAADGIGG
                     LGSESDAVRGSDGQPHNFSPTLEASFATSALFGFVFSYADGAAFYTGTVAVAPGSTPA
                     VPSGGILGSYSVFAEGVTPRDAGTVAVDRFTAGGASYVPDGAAGGAGGLGSEAGSITV
                     NGTTFVFSDRQEPVIPSGIASVPLDPTPDPNDVVASILTEIYREVLDRLPDPGGLATY
                     RAGLAGGGSAASVRRDIAASDEAGGLINGLYHQIFGRDADPVGLAIYRGALANGSSL"
BASE COUNT          244 a          671 c          565 g          214 t
ORIGIN      
        1 aggctcgacc cgttggccag cgccccgcgg tagatcgcca gcccgaccgg gtccgcatcg
       61 cgcccgaaaa tctggtggta cagcccgttg atcagacccc ccgcctcgtc cgaggcggcg
      121 atgtcccggc gcaccgaggc cgcgctgccg ccgcccgcca gccccgcccg gtaggtcgcc
      181 agaccgcccg ggtcgggcag gcggtccagc acctcccggt agatttcggt gaggatgctg
      241 gcgacgacgt cgttggggtc cggcgtcggg tcgagcggca ccgaagcgat gccggacggg
      301 atgaccggct cctgccggtc gctgaagacg aacgtcgtgc cgttgacggt gatcgacccc
      361 gcctccgagc ccagcccgcc cgcgccgccg gccgcgccgt ccgggacata ggacgcgccg
      421 cccgcggtga agcggtcgac cgccaccgtg ccggcgtcgc gcggcgtcac cccctcggca
      481 aataccgaat agctgcccag gatgccgccc gaggggaccg ccggggtgct gccgggtgcc
      541 accgccacgg tccccgtgta gaacgccgcg ccgtcggcat agctgaagac aaagccgaac
      601 agcgcactcg tcgcgaaact cgcctccagc gtcgggctga aattgtgcgg ctgtccgtcg
      661 ctgcccctca ccgcgtcgct ttcgctgccc agcccgccga tgccgtcggc cgccgggaag
      721 ccgagcggaa cgggcgaggc cgcgcccggc ccgtcatgcg aataattgct gacaaagacc
      781 gacccggccg ccaccggcgc cggcagccct tccttgtcaa agaccgtgta ttggcccaat
      841 ccgacggaga tatgctgccc gacatgatag ccgaacgtgc cgttatcggc ggcccggccg
      901 tagtaatagt ccttgccgtc accgtaggcg aagaggaagt cgaagaagct gaccgtcgcc
      961 gacggcacga ccgtcagcgt cgcgccgccc gccgccgtgt tgcccgcccg atcggtgacg
     1021 ctgtagccga agctgccgcc cgccgccggc cggtccgccg ccggggtgaa gagcagcccg
     1081 ctcagctgcc cgatcgtcag cggctcgcct gccgtcaccg gggtgacgcc gtcgccgagg
     1141 gtgagcgtgc cgttgcccgg caggccggtg accgtgacgc cgagcgcgtc cgccggatcg
     1201 tcgctgtcgc tcggcgacgc aatcccgatc ggcgccgcgc cgccgtcaaa ggcaaagctc
     1261 gccgagacca ccgccgtcag cggcgccgag gtgtcgatgg tcaccgagag ccccgccgag
     1321 ggcaggctca cctcgcccgc gccgtcgctc tcgctcgcgg tcaacagatg caccccggcc
     1381 gcgaggctcg gcgccagaac ccacccgccg ccggccccga cgatcgtgct ctggccggtg
     1441 tccgccccgc cgtcgaagag ccgcaccctg tcgccggcct cgccgctgcc gccgatcagc
     1501 ggcaccgtga cattggtccg gttgtcgccc ggggtgccgc tatccgacag cagcaccagc
     1561 cccgacggcg ccggcggcac ctcgtgcacc tcggtcccgc cccgcccgtc atccgacagc
     1621 gcaaacgccg tccccggcgc gacgccaatg aggctcagcg tgtcgaccac cccgccgccg
     1681 ctgaccgtca gcgc
//
LOCUS       DASPYY010000090         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_295415, whole genome shotgun sequence.
ACCESSION   DASPYY010000090 DASPYY010000000
VERSION     DASPYY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295415"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEP73_01270"
     CDS             <1..387
                     /locus_tag="VEP73_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25103.1"
                     /translation="DNAAKYAPAGVPVWVDALRDGSLVVLAVEDGGPGVPTAERERIF
                     DRFGKLDANAVRRAGGVGLGLYIARRLARAEGGDLLLVEPTRPDAGARFELRLPPALK
                     WEARSVADAVAEGIAKDTESRTSHLF"
     gene            complement(411..>1540)
                     /locus_tag="VEP73_01275"
     CDS             complement(411..>1540)
                     /locus_tag="VEP73_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HYT25104.1"
                     /translation="YLPLAHSLERLSGHYAALWRAESVWFCPEVLRVVEYLPQVRPST
                     FVAVPRLWEKAQAGIAAVLAAEPNPRRRRIAEQAIATGRQAARLAQAGKPVPPGLRLR
                     RALFDRLVFSKIRHRLGLDRCLVAFSGAAPLAEDVMEFFLAIGVPLREGYGLTESTAP
                     ASLNLPERVKVGTVGPALPGVELRVADDGELLIRGGNVAKGYYKDPEQTAATFDPDGW
                     LHSGDVAEVDEDGFIRIVDRKKELLITAGGDNVSPSNLENLLKAHPLIAQACAVGDRK
                     PYIAALIVLDGEVAPAWAAQHGIPFQSLEQLAADERVRAELQSAVDAVNQHVARIEQL
                     KRFTVLPAEWTVDSEELTPTLKLKRRVIHKKYAAEIQALYQ"
BASE COUNT          203 a          542 c          575 g          220 t
ORIGIN      
        1 gacaacgcgg ccaagtatgc acccgccggg gtgccggtct gggtcgacgc gctgcgcgac
       61 gggagcctcg tggtcctcgc ggtcgaggac ggcggcccgg gggtgcccac cgccgagcgc
      121 gagcgcatct tcgaccgctt cggcaagctc gacgccaatg ccgtccgccg tgccggcggg
      181 gtcgggctgg gcctgtacat cgccaggcgc ctcgcccgcg ccgaaggcgg cgacctgctg
      241 ctggtcgagc cgactaggcc cgacgcgggc gcccgcttcg agctgcgcct cccgcccgcc
      301 ctcaaatggg aggcccgcag cgtcgccgac gccgtcgccg aaggcatcgc caaagacacg
      361 gagtccagga ccagccatct cttctgagcc ctggggtctg caccgaggcc ctactggtac
      421 agcgcctgga tctccgcggc gtacttcttg tgaatcacgc ggcgtttgag cttgagggtg
      481 ggggtcagct cctcggagtc gacggtccac tcggccggca ggacggtgaa gcgcttgagc
      541 tgctcgatgc gggcgacgtg ctggttgacg gcatcgacgg cggactgcag ctcggcgcgg
      601 acgcgctcgt cggcggcgag ctgttctagg ctctggaagg ggatgccgtg ctgggctgcc
      661 caggcggggg cgacctcgcc gtccagcacg atcagcgcgg cgatgtaggg cttgcggtcg
      721 ccgaccgcgc acgcctgggc gatcagcggg tgcgccttca gcagattttc gagatttgag
      781 ggcgagacgt tgtcgccgcc ggcggtgatc agcagctcct tcttgcggtc gacgatgcgg
      841 atgaagccgt cctcgtccac ctcggcgacg tcgccggagt gcagccagcc gtccgggtcg
      901 aaggtcgcgg cggtctgctc ggggtccttg tagtagccct tggcgacgtt gccgccgcgg
      961 atcagcagct cgccgtcgtc ggcgacgcgc agctcgaccc cggggagcgc cggccccacc
     1021 gtgccgacct tgacccgctc gggcaggttg aggctggccg gcgcggtcga ctcggtcagg
     1081 ccgtagccct cgcgcagcgg cacgccgatg gccaggaaga actccatcac gtcctcggcg
     1141 agcggggcgg cgccgctgaa cgccaccagg cagcggtcca ggccgagccg gtggcggatc
     1201 ttgctgaaca ccagccggtc gaacagggcg cggcgcaggc gcagcccggg ggggaccggc
     1261 ttgcccgcct gggccagtcg cgccgcctgc cggccggtcg cgatcgcctg ctcggcgatg
     1321 cggcggcggc gcgggttcgg ctcggcggcc agcaccgcgg cgatgccggc ctgcgccttc
     1381 tcccacaggc gcgggactgc cacgaaggtg gagggccgga cctgcggcag gtactcgacc
     1441 accctgagca cctccgggca gaaccacacc gactccgccc gccacagggc ggcgtagtgc
     1501 ccgctcagcc gttccaggct gtgtgccagc ggcaggtagg
//
LOCUS       DASPYY010000091         4586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_436309, whole genome shotgun sequence.
ACCESSION   DASPYY010000091 DASPYY010000000
VERSION     DASPYY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436309"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VEP73_01280"
     CDS             complement(<1..139)
                     /locus_tag="VEP73_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004955763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT25105.1"
                     /translation="MAEVKSYAPGTPSWVDLMTSDPEGARAFYGELFGWEFEIGGPET
                     GN"
     gene            complement(309..1790)
                     /locus_tag="VEP73_01285"
     CDS             complement(309..1790)
                     /locus_tag="VEP73_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT25106.1"
                     /translation="MADGITAAPGRAPARLDWAYAPAPEATDHVKVRGRYGLLVDGKW
                     VEAASGSRFETINPATEQVLASVAEAGEEDVDAAVKAARTAWAKVWSKTSGVERAKYL
                     YRIARILQERAREFAVLETMNGGKPIKESRDVDVPLAAAHFFYHAGWADKLDYAFPGR
                     RPRPLGVAAQVIPWNFPLLMAAWKLAPALAAGNTVVLKPAETTPLTALLLGEVLQQAE
                     LPPGVVNIVTGAGQTGAALVAHPGVDKVAFTGSTEVGKAIQRTLAGSGRRLTLELGGK
                     AANIVLDDAPIDQAVEGIINGIYFNQGHVCCAGSRLLVQESVAEPLVAKLKRRMATLR
                     VGDPLDKNTDVGAINSRAQLDRIEELVASGEADGAEVYQPPCELPDRGFWFRPTVFTG
                     VAQSYRIAQEEIFGPVLSVLTFRTPDEAVQKANNTPYGLSAGVWTDKGSRILELASRL
                     RAGVVWANTFNRFDPASPFGGYRESGFGREGGRHGLAAYLDLS"
     gene            complement(1832..2785)
                     /gene="deoC"
                     /locus_tag="VEP73_01290"
     CDS             complement(1832..2785)
                     /gene="deoC"
                     /locus_tag="VEP73_01290"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HYT25107.1"
                     /translation="MATTPVRLPEAGGGFLQDGALGSLLARSAVDQTGVEERAASLAK
                     RSIKKDAKVAALRLAVAVTDLTTLEGADTVGKVEALCAKARRPDPSDPGVPPVAAVCV
                     YPRLAATAKRALAGSGVKVASVATAFPSGQSSLAVRLADVADAVDAGADEVDMVISRG
                     AFLAGRYRDVFEEILIVKDACGPAHLKVILETGELGSYDDVRRASLLAMAAGADFVKT
                     STGKVQPAATLPVALVMIEAIRDFHDRTGRAVGFKPAGGVRTAKQAIQHLVLVNETLG
                     PDWLTPDRFRIGASSLLNDCLMQLAKERTGRYQSANYFTVD"
     gene            3080..3613
                     /locus_tag="VEP73_01295"
     CDS             3080..3613
                     /locus_tag="VEP73_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25108.1"
                     /translation="MRSLGRGFALVLAGAFLLGVYKTHAPGAKEGPSLGQPTGTAAVV
                     RAHQNRTPPRLVSVRTSSHGTFDRVVFTFAGPLPASRTEFVRSVRAEGSATPLRLSGF
                     ADLRVTFAAVGRDRAGRPTWPGATSRRTAYPSLRQVTLAGSGGGQTTFGLGLDHKVGF
                     RVIELARPTRIVIDVAS"
     gene            3673..4377
                     /locus_tag="VEP73_01300"
     CDS             3673..4377
                     /locus_tag="VEP73_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25109.1"
                     /translation="MGLDVYVGSLTGWYAAGPPAAVERLAWHQRVPYMVIRGSEHASG
                     HTERDVIRSAVLAWRDALRQALGDRVEAPLDWDESPEAPSFTGRPGWDGYGATVLLAA
                     YEEHPELEAPATVAPEWPDDVAYQTSVAAGACSRYDHLLVPDLWLPCPFRFTFRTQDA
                     AGHTVDVGSSTTLLAQLRTLGARTGLATPDTGGEVPRPAGPKPPLRATARHGLAALLR
                     LAGRSVEHRVPMRLDH"
BASE COUNT          575 a         1747 c         1636 g          628 t
ORIGIN      
        1 agttcccggt ctcggggccg ccgatctcga actcccatcc gaacagctcg ccgtagaagg
       61 cgcgcgcccc ctccgggtcg ctcgtcatga gatcgaccca cgagggcgtc cccggcgcat
      121 acgacttgac ctcggccatg ctccccctcc tgctccatcc gctggagcct ccggttgccg
      181 gaaccgctgg tttcccgaac gtgcgttcga gaccgccgta tcctaccgca cgcgcccgat
      241 cttctctcct cgaagcggac gcgcccggca ttgccacccg ccgttcacgg cgcggaggag
      301 gtcacccttc agctgaggtc caggtaggcg gcgaggccgt gccggccgcc ctcccggccg
      361 aagcccgact cccggtagcc gccgaacggc gacgccgggt cgaaacggtt gaacgtgttg
      421 gcccagacca cgccggcccg caggcggctg gcgagctcga ggatgcggct gcccttgtcg
      481 gtccacaccc ccgccgacag gccgtacggg gtgttgttgg ccttctggac cgcctcgtcg
      541 ggagtgcgga acgtcagcac cgacagcacc gggccgaaga tctcctcctg ggcgatgcgg
      601 taggactggg cgacgccggt gaacaccgtc ggccggaacc agaagccgcg gtcgggcagc
      661 tcgcacgggg gctggtagac ctccgcgccg tcggcctcgc ccgaggcgac cagctcctcg
      721 atcctgtcga gctgggcccg cgagttgatc gcgcccacgt cggtgttctt gtcgagcggg
      781 tcgcccaccc gcagggtggc catgcgccgc ttgagcttgg cgaccagcgg ctcggcgacc
      841 gactcctgca ccagcaggcg cgacccggcg cagcagacgt ggccctggtt gaagtagatc
      901 ccgttgatga tgccctcgac cgcctggtcg atcggggcgt cgtcgagcac gatgttggcc
      961 gccttgccgc cgagctccag ggtgagccgc ctgccggagc cggccagggt ccgctggatc
     1021 gccttgccga cctcggtcga accggtgaag gcgaccttgt cgacgcccgg gtgggcgacc
     1081 agcgcggcgc cggtctgccc ggcgccggtg acgatgttga ccaccccggg cggcagctcg
     1141 gcctgctgta gcacctcgcc gagcagcagc gcggtgagcg gcgtggtctc ggccggcttg
     1201 agcacgacgg tgttgccggc cgcgagcgcg ggcgcgagct tccaggccgc catcagcagc
     1261 gggaagttcc acgggatgac ctgcgcggcg acgccgagcg ggcgcgggcg gcggcccggg
     1321 aaggcgtagt cgagcttgtc ggcccagccg gcgtggtaga agaagtgggc cgccgccagc
     1381 gggacgtcga cgtcgcggga ctccttgatc ggcttgccgc cgttcatcgt ctcgaggacg
     1441 gcgaactcgc gggcacgctc ctgcaggatg cgggcgatcc ggtacaggta cttggcccgc
     1501 tcgaccccgg aggtcttcga ccacaccttg gcccaggcgg tccgggccgc cttgacggcc
     1561 gcgtcgacgt cctcctcccc cgcctcggcg accgaggcga ggacctgctc ggtggccggg
     1621 ttgatggtct cgaaccgcga gccggaggcc gcctcgaccc acttgccgtc caccagcagc
     1681 ccgtagcgcc cgcgcacctt gacgtggtcg gtcgcctcgg gcgcgggcgc gtaggcccag
     1741 tccaggcgtg ccggcgccct gccaggcgcc gcggtgatcc cgtcggccat cgtctcggcc
     1801 atcctcggcc tcccgtccgg tcccatgtgg ttcagtcgac cgtgaagtag ttggccgact
     1861 ggtagcggcc ggtgcgctcc ttggcgagct gcatgaggca gtcgttcagc aggctcgacg
     1921 cgccgatccg gaaccggtcc ggggtcagcc agtccggccc gagggtctcg ttcaccagca
     1981 cgaggtgctg gatcgcctgc ttggcggtgc gcacgccgcc ggccggcttg aacccgaccg
     2041 cccggccggt gcggtcgtgg aagtcgcgga tcgcctcgat catgacgagg gcgacgggca
     2101 gggtggccgc gggctggacc ttcccggtcg aggtcttgac gaagtccgcc ccggccgcca
     2161 tggcgagcag gctcgcacgg cgcacgtcgt cgtagctgcc gagctcgccg gtctccagga
     2221 tgaccttgag gtgggccggg ccgcaggcgt ccttgacgat caggatctcc tcgaacacgt
     2281 cgcggtagcg gccggccagg aacgcgcccc ggctgatcac catgtcgacc tcgtcggcgc
     2341 ccgcgtcgac ggcgtcggcg acgtcggcca gccggaccgc gagcgacgac tgcccgctcg
     2401 ggaacgcggt cgccaccgag gcgaccttca ccccggaccc ggccagcgcg cgcttggcgg
     2461 tggcggcgag ccgcgggtag acgcagaccg ccgcgacggg cggaaccccg gggtcggagg
     2521 gatcgggccg cctcgccttg gcgcagaggg cctccacctt gccgaccgtg tccgcccctt
     2581 ccaaggtggt gaggtccgtc acggccaccg cgagccgcag ggcggccacc ttcgcgtcct
     2641 tcttgatgct gcgcttggcg agcgacgcgg cgcgctcctc gacgcccgtc tggtcgacgg
     2701 cggaccgtgc caacagcgag ccgagcgcgc cgtcctgcag gaagccgccg ccggcctcgg
     2761 ggagccggac gggtgtcgtt gccatgccac ctcccgcgcc cggggcctca ccgctgtcgg
     2821 gtccgccgtc gagccaccat cgagatcgag ccgccatcga gccgtcggtc gattgtagag
     2881 cgcccgggcg gcggtcgttc gttcccgcgg aacacgacga ccacccgcgc aacgacgaag
     2941 ctgtcatcat cttgtgaccc tgggcggtca tgatggtctc ggacccgttt ctacactggt
     3001 tccaccctgg tgggaccggc cgtaccgggt cgccggcagc cgtgccgggt tgcccggtag
     3061 cggaagggag acgcacggcg tgcgatcgct cgggagaggc ttcgccctgg tcctggccgg
     3121 cgcgttcctg ctcggcgtct acaagacgca cgccccgggc gcgaaggaag gcccgtcgct
     3181 cgggcagccg accgggaccg ccgcggtggt ccgcgcgcac cagaaccgga cgccgcccag
     3241 gctcgtgtcg gtgcggacct cgagccacgg caccttcgac cgcgtcgtct tcaccttcgc
     3301 cggcccgctt cccgcctccc ggaccgagtt cgtgcgcagc gtccgcgccg aggggtcggc
     3361 cacgccgctg cgcctgagcg gcttcgccga cctgcgggtc accttcgcgg ccgtgggccg
     3421 cgaccgcgcg ggcaggccga cctggcccgg cgcgaccagc cgccgcacgg cgtacccgtc
     3481 gctgcggcag gtcaccctgg ctggcagcgg cggcgggcag accacgttcg ggcttggcct
     3541 cgatcacaag gtcgggttcc gggtgatcga gctggcccgg cccacccgca tcgtgatcga
     3601 cgtcgcgtcc tgaccgaacc acccagatcc accgacgcct tggccgaccg gtcgtgacag
     3661 gaagggagca gcgtgggcct tgacgtctat gtcggctccc tgaccggctg gtacgccgcc
     3721 gggccgccgg cggccgtcga gcgcctggcc tggcaccagc gcgtccccta catggtcatc
     3781 cgcggcagcg agcacgcgag cgggcacacc gagcgggacg tgatccgctc cgccgtgctg
     3841 gcgtggcgcg acgccctgcg ccaggcgctc ggcgaccggg tcgaggcgcc gctcgactgg
     3901 gacgagtcgc ccgaggcgcc gtcgttcacc gggcggcccg gctgggacgg ctacggcgcc
     3961 acggtgctgc tcgccgccta cgaggagcac ccggagctcg aggccccggc cacggtcgcc
     4021 ccggaatggc ccgacgacgt cgcctaccag acgtccgtcg ccgcgggcgc ctgctcccgc
     4081 tacgaccacc tcctcgtgcc cgacctgtgg ctcccctgcc cgttccggtt caccttccgg
     4141 acccaggacg ccgccgggca caccgtcgac gtcgggtcga gcaccaccct gctcgcccag
     4201 ctccggaccc tgggcgcccg gaccgggctc gccaccccgg acaccggcgg cgaggtcccc
     4261 cgccccgccg gcccgaagcc gcccctgcgc gcgaccgccc gccacgggct cgcggccctg
     4321 ctccggctgg cgggccgctc ggtcgagcac cgggtcccga tgcgcctcga ccactgaccc
     4381 acccgccgcg gcggccggga ccaggacccg cgcggcgcgc cggagccggg accgcgcggc
     4441 catctcagag gggcggcggg ccctcgagaa cctcgacgcg ggcgccccac gggtcgcggg
     4501 tgaagaagcg gcgccggccc gcccaggcgt ggtcgtcctc gaccccgccc ccggccgcct
     4561 ctgcgcggcc gcgcagcgcg tcgaag
//
LOCUS       DASPYY010000092         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2543122, whole genome shotgun sequence.
ACCESSION   DASPYY010000092 DASPYY010000000
VERSION     DASPYY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543122"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1027)
                     /locus_tag="VEP73_01305"
     CDS             complement(203..1027)
                     /locus_tag="VEP73_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYT25110.1"
                     /translation="METLLLERRGPVAWLRLDRPAKRNAQTRAMWDELRAAGAELVHD
                     LAVRCLVVTGEGPSFSAGIDLTELAGGVGGLAGAAGRGADPDGEVGGDPLPAAIRHIQ
                     TAFTWIRAAPFPTVAAVRGHALGAGLQLALACDLRVFASDAVVGLVELRWGILPDLGG
                     TEWLPGIVGAAKARELVLTAAEVDAEEALRIGLANRVVAPEELEAATTTLAGTLAAQP
                     PLAVRHAKRALDAAFEATGRSLELAAAGQAECLRSADFVEAVRAAAERRPPVYQGR"
     gene            complement(1107..>2265)
                     /locus_tag="VEP73_01310"
     CDS             complement(1107..>2265)
                     /locus_tag="VEP73_01310"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25111.1"
                     /translation="YVAIPYQLFQLTRSSLAVGLLGIVELAPLLATALLGGALADARD
                     RRRMVQLTELGLAALSGALVLNAMAGRPRVWVLYAVAALAAALDGLQRPSLDALVPRL
                     VGRDEMPAAAALSSLRMNFGMVGGPAVGGLLVAKLGLAATYGLDVVSFVASLVALRLM
                     RAVPPPAGAERPSLRGVLRGLRYARSRQELLGTYVVDIVAMLFGMPTALFPAVAVRYG
                     GAGALGLLYAAPSVGSLLANATSRWTGGVHRHGRAILLAAAVWGAAIAAFGLARPLWL
                     ALGLLAVAGGADMVSGIFRSTIWNQTIPDPLRGRLAGIELLGYSTGPLLGNVEAGGVA
                     AVFGVRASVVSGGLLSVVGVAVVAVAMPVFRTYDARRRPAPEPGAPVPETT"
BASE COUNT          317 a          859 c          873 g          216 t
ORIGIN      
        1 cccggcggca ccgagcacgt gaccagcacg cctcggacgc cggttgcggt ctggacctgg
       61 ctcggggaat gggcggtcgc cgtgcggctc ggccccgcca accagttggt ggtcggccgg
      121 cacaccggcg cccgctgctg aacgaccgga agcgcccgga gtcccacccc gggcgcttcc
      181 ggcgctccag ggcatgtgcc ggtcagcggc cctggtacac cggcggccgc cgctcggcgg
      241 cggcgcggac ggcctcgacg aagtcggccg agcgcaggca ctcggcctgg ccggcggcgg
      301 ccagctccag gctgcggccg gtcgcctcga atgccgcgtc gagcgcccgc ttggcgtgcc
      361 gcacggccag gggtggctgg gcggccagcg tcccggccag cgtggtggtg gccgcctcca
      421 gctcctcggg ggcgaccacc cggttggcca ggccgatgcg cagcgcctcc tcggcgtcca
      481 cctcggcggc ggtcaggacg agctccctcg ccttggcggc accgacgatg ccggggagcc
      541 actcggtgcc gccgaggtcg ggcaggatcc cccaacggag ctcgacgagc cccaccacgg
      601 cgtcggacgc gaagacgcgc aggtcgcacg cgagcgcgag ctgcagcccg gcaccgagcg
      661 cgtggccgcg gaccgcggcg acggtcggga acggcgcggc gcggatccag gtgaacgcgg
      721 tctggatgtg gcggatcgcg gccggcagcg ggtccccgcc gacctcgccg tcggggtcgg
      781 cgccacggcc ggccgccccc gccaggccgc cgacgccacc cgccagctcg gtgaggtcga
      841 tcccggccga gaagctcggt ccctcacccg tcaccaccag gcagcgcacg gcaaggtcat
      901 ggacgagctc cgcaccggcg gcccgcagct cgtcccacat ggcgcgcgtc tgggcgttcc
      961 gtttggccgg ccggtcgagc cgcagccagg ccaccggccc ccgccgctcg agcaagaggg
     1021 tctccatcgg cttcctcctc ggtcgccccg tgcgagggtg cccggagccg cacaagtatc
     1081 gccccgcgcc aggcgggtcc ggcgcctcag gtggtctccg gaaccggcgc gcccggctcc
     1141 ggggccggcc ggcgacgcgc gtcgtaggtc cggaacaccg gcatcgcgac ggccacgacc
     1201 gcgaccccga cgacactgag cagtcccccc gacaccaccg aggcgcggac gccgaacacc
     1261 gcggcgaccc cgcccgcctc cacgttgccg agcagcggcc cggtcgagta gccgagcagc
     1321 tcgatgcctg ccaggcggcc acgcaggggg tccgggatgg tctggttcca gatcgtcgaa
     1381 cggaagatgc cgctcaccat gtcggccccg cccgccacgg cgagcaggcc gagggcgagc
     1441 catagcggcc gggcgagccc gaacgcggcg atggcggcgc cccagaccgc ggccgcgagc
     1501 aggatggcgc gaccgtggcg gtggacgccg ccggtccagc ggctggtcgc gttggcgaga
     1561 agggagccca ccgaaggggc ggcgtagagc aggccgagcg cgccggcccc gccgtaacgg
     1621 acggcgacgg ccgggaacag cgcggtcggc atgccgaaca gcatcgcgac gatgtcgacg
     1681 acgtaggtgc cgagcagctc ctggcggctg cgggcatagc ggagcccccg cagcacgccg
     1741 cgcaggctcg gccgctccgc gcccgccggg ggcggcacgg cccgcatgag gcggagcgcc
     1801 acgagcgagg cgacgaagct gaccacgtcg agaccatagg tggcggcgag accgagcttg
     1861 gccaccagca ggcccccgac ggccgggccg cccaccatcc cgaagttcat ccgcagcgag
     1921 ctgagcgcgg cggcggccgg catctcgtcg cgcccgacga gcctcggcac gagcgcgtcg
     1981 agcgaagggc gctgcaggcc gtccagcgcg gccgcgaggg cggcgacggc gtagaggacc
     2041 cagacgcgcg ggcgtccggc catggcgttg agcaccagcg ccccggagag ggcggcgagc
     2101 ccgagttcgg tgagctgcac catccgccgc cggtcccggg cgtcggccag cgccccgccg
     2161 aggagggcgg tggccagcag cggggcgagc tcgacgatcc cgagcaggcc cacggcaagc
     2221 gaggagcggg tgagctggaa cagctggtac gggatggcga cgtag
//
LOCUS       DASPYY010000093         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_858322, whole genome shotgun sequence.
ACCESSION   DASPYY010000093 DASPYY010000000
VERSION     DASPYY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858322"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VEP73_01315"
     CDS             complement(<1..1311)
                     /locus_tag="VEP73_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYT25112.1"
                     /translation="MDVGVDEEMVVVDYTVAGVEGGVCAPRGFRAAGVDAGLVDGPGS
                     DLGLVVSQQSAAAAGRFTGHRFASAPVRWSRLQVAAGAARAVLVHAGAANAATGTRGD
                     ADAAALAELCAAAVGCTATEVLLLGTGPIGRRLELEAAAEGAEKAVAALGRRGSTELA
                     RALAASGGVAREHAVAVAWSEGEVRIGGVAKASVPFAPSFASTLVLLTTDATVEPAVL
                     DGILERALGRSFERILVDQGPSMADSAILLANATAAAPVLSAGSEGAAAFEAALAYLC
                     ESLGEQLAMRIPGSRKLIVVTVHGAASHADALAAARAVAGSVALRAALAAGLPAWPAV
                     LDAIDRAAIPLDPTLVGVRFGPVTVVAGGEAAAHDERAAAAVAAKGQVDLAVDLGAGE
                     AAVWAAEPLQHLGAAEAGHHHVEDHHGGEDRAGEPQPLLAVGGED"
     gene            complement(1370..3889)
                     /gene="pheT"
                     /locus_tag="VEP73_01320"
     CDS             complement(1370..3889)
                     /gene="pheT"
                     /locus_tag="VEP73_01320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYT25113.1"
                     /translation="MRVPLTWLAEHVDLGDIGPEALADKLTFAGLEIETIDWVGHDIA
                     GVITARVLDVTRHPDADRLALVTIDPGTEPREVVCGAHNYGPGDVVPWAAPGAKLPGG
                     LEIGRRKVRGRWSDGMLASARELGVFDDHAGILVLPPDTPIGADLTDEAIGLRDAVLD
                     IKTFPNRGDTLSIRGIAREAALVLGSELRPLDLSVPEAGPPAARLASVEVDDTEGCPV
                     YHARVAEGLDAARPAPLWMARRLYLYGQRPLGAVVDVTNYLLIDQGQPLHAFDLDRIP
                     GQRILVRRARPGEVVRTLDGRDRHMTPDDTLITAGEEPLGLAGIMGGEDSEVRADTTR
                     VLLESAHFPPASIRRTMRRLGMNTEGAQRWARGVDPAGAGPVCDQAARLMAALAGGTI
                     ASGRLRAGPGVREREAISLNWRRSAERLGAPADPAFAAAYLDSVGCTVRVEGGPDAIV
                     AAVPPSWRFDLEGWADLEEEVARRWGYDKLPATLPKATGGRRSAEQRLRRHTRELLAG
                     MGLTEVQPYPFSSQAALDRLELPASDRRRRALRLANPLSEEYPELRTTLLPGLAEVAR
                     RNLEKGLAGVAVYEIGALFLPDPDGNRELPDEPRMLGILLAGRRLGGRYDDPAPEVTF
                     ADAKGVVEGLAAALGVEQVSYRPEAPMPYHPGRCAGIWLGGGAGGSGSSGDDEGVPIG
                     VVGQLHPRVARNLELPAATFAAELELTPLLEAAPATRPAPTPSPYPELSFDVAFLVPP
                     GVAAADLQATLADAGADLLARLALFDAYEGPPLPPGHRNLAFRVTLQAPDRTLTDAEA
                     ATTRDRMVTLAAERHDAALRSGATGLSTDVALGDAGDRAAP"
     gene            complement(3931..>4581)
                     /locus_tag="VEP73_01325"
     CDS             complement(3931..>4581)
                     /locus_tag="VEP73_01325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYT25114.1"
                     /translation="PEVETDWYNFEALNFPPDHPARSMQDSVSLQARDAQGIPLLLRT
                     HTSPMQIRSMLAAPPPLYVVIPGRCYRADTPDATHVPVFNQIEGLAVDEHITMGDLKG
                     TLLAFARAYFGEERQIRLRPSYFPFVEPGAEVDVSCFVCGGRGTGCRTCRGEGWIELL
                     GAGMVHPNVLRAGGYDPDRVSGFAFGMGIERPYMLRSGLADMRTLTDNDLRWLANA"
BASE COUNT          561 a         1564 c         1880 g          576 t
ORIGIN      
        1 gtcctcgcca ccgacggcga ggaggggctg cggctcgccc gcgcggtcct ccccgccgtg
       61 gtggtcctcg acgtgatgat gcccggcctc ggcggccccg aggtgctgca gcggctccgc
      121 cgcccagacg gcagcctccc cggcgccgag atcgacggcg aggtcgacct ggcctttggc
      181 ggcgactgcg gcggcggcgc gctcgtcgtg ggccgcggct tcgccaccgg ccaccaccgt
      241 gaccggtccg aagcggaccc cgaccagggt cgggtcgagc gggatcgcgg cccggtcgat
      301 ggcgtcgagc accgccggcc aggcaggcag gccggcggcg agtgcggcgc gcagcgccac
      361 cgacccggcc accgcgcggg cggcggcgag cgcgtcggcg tggctggcgg cgccgtggac
      421 ggtgacgacg atcagtttgc ggctgccagg gatccgcatg gcgagctgct cgccgaggga
      481 ctcgcacagg taggcgaggg cggcctcgaa cgcggcggcg ccttcggagc cggcgctgag
      541 caccggcgcg gcggcggtgg cgttggcgag caggatggcg ctgtcggcca tactggggcc
      601 ctggtcgacc aggatccgct cgaaggagcg gccaagcgcc cgttcgagga tgccgtcgag
      661 cactgccggt tccacggtgg cgtcggtggt gagcagcacc agggtgctgg cgaacgaggg
      721 cgcgaacggg accgatgcct tggcgacccc gccgatgcgg acctcgcctt cggaccaggc
      781 gacggcgacg gcatgttcgc gggcgacacc gccggatgcg gccagggcgc gggcgagctc
      841 ggtggagccg cgccggccga gcgcggcaac ggccttctcg gcgccttcgg cggccgcttc
      901 gagctctagg cgccggccga tcgggccggt gccgagcagc aggacctcgg tggcggtgca
      961 gccgaccgcg gcggcgcaca gctccgccag ggcggcggcg tcggcgtccc cgcgggtccc
     1021 ggtggcggcg ttggcggcac cggcgtggac caggacggcg cgggcggccc cggcggcgac
     1081 ctgcagccgt gaccagcgca ccggcgccga ggcgaagcgg tggccggtga agcggcccgc
     1141 cgccgccgcg ctctgttggc tcaccaccag gccgaggtcg gacccgggtc cgtcgaccag
     1201 cccggcgtcc acccccgccg cgcggaaccc ccggggcgcg cagacaccac cctccactcc
     1261 ggcgaccgtg tagtcgacca cgaccatctc ctcgtcaaca ccgacatcca cgccgttcgg
     1321 cgcattctga ccgaaccccc tgccggtggg ggtccccccg gtttcaagcc tacggcgcgg
     1381 cgcggtcgcc ggcatccccc agcgcgacat ctgtggataa cccagtggcg ccgctgcgca
     1441 gggcggcgtc gtggcgctcg gcggcgaggg tgaccatgcg gtcgcgggtg gtggccgcct
     1501 cggcgtcggt gagggtgcgg tcgggggcct gcagggtgac ccggaaggcg aggttgcggt
     1561 ggccgggagg cagcggcggg ccctcgtagg cgtcgaacag ggcgaggcgg gcgagcaggt
     1621 ccgcgccggc gtcggcgagg gtcgcctgca ggtcggcggc ggccacgccg ggtgggacca
     1681 ggaaggccac gtcgaagctg agctccgggt agggcgaggg ggtgggcgca ggccgggtgg
     1741 cgggcgccgc ctccagcagc ggggtcagct ccagctcggc agcgaaggtc gcagcgggca
     1801 gctccaggtt gcgggcgacg cgggggtgga gttgcccgac aacgccgatc ggcacgccct
     1861 cgtcatcacc gctgctgccg ctgcccccgg ccccgccgcc gagccagatc ccggcgcagc
     1921 ggccggggtg gtagggcatg ggcgcttcgg ggcggtagct gacctgctcg acgccgagcg
     1981 cggcggccag gccctcgacc acgcccttgg cgtcggcgaa ggtgacctcg ggggcggggt
     2041 cgtcgtagcg gccgccgagg cggcggccgg cgagcaggat cccgagcatg cggggctcgt
     2101 cgggcagctc ccggttgccg tccgggtcgg gcaggaacag cgcgccgatc tcgtagacgg
     2161 ccacgccagc aaggcccttt tcgaggttgc gcctggccac ctcggcgagg ccgggcagca
     2221 gtgtggtgcg cagctccggg tactcctcgg acagcgggtt ggcgagccgc agggcgcggc
     2281 ggcgccggtc gctggcaggc agctccagac ggtcgagggc ggcctggctg gagaacgggt
     2341 agggctgcac ctcggtgagg cccatgccgg ccagcagctc gcgggtatgg cggcgcaggc
     2401 gctgctcggc gctgcggcgg ccgccggtcg ccttcggcag ggtcgcgggg agcttgtcgt
     2461 agccccagcg gcgggcgacc tcctcctcca ggtcggccca gccttccagg tcgaagcgcc
     2521 agctcggcgg gacggccgcc acaatggcgt ctggtccgcc ctccacccgc acggtgcagc
     2581 cgacgctgtc gaggtaggcg gcggcgaagg cggggtcggc gggcgcgccg agccgctcgg
     2641 cggagcggcg ccagttgagg ctgatggcct cgcgctccct caccccgggg ccggcgcgga
     2701 gccggccgct ggcgatggtc ccgccggcga gcgccgccat gaggcgggcg gcctggtcgc
     2761 agacgggccc ggcgccggcc gggtcgacac cgcgggccca gcgctgggcg ccttcggtgt
     2821 tcatgccgag gcggcgcatg gtgcggcgga ttgaggctgg cgggaagtgg gccgattcga
     2881 ggaggacccg ggtggtgtcg gcgcggacct cggagtcctc gccgcccatg atgccggcca
     2941 gcccgagcgg ctcctcccca gcggtgatga gggtgtcgtc gggggtcatg tggcggtcgc
     3001 ggccgtcgag ggtgcggacc acctcgccgg gccgggcgcg gcgcaccagg atccgctggc
     3061 cggggatgcg gtccaggtcg aaggcgtgca gcggctggcc ctggtcgatc agcaggtagt
     3121 tggtgacgtc gacgacggcg ccgaggggcc gctgtccgta caggtagagg cggcgggcca
     3181 tccacagcgg cgcgggccgg gccgcgtcga ggccctccgc gacccgcgcg tggtacaccg
     3241 ggcagccttc ggtgtcgtcc acctcgacgc tggccagcct ggcggcaggg ggacctgcct
     3301 cgggcacgga gaggtcgagc ggccgcagct cgctgccgag caccagggcg gcctcgcggg
     3361 cgatgccgcg gatggacagg gtgtcgccgc ggttggggaa ggtcttgatg tcaaggacgg
     3421 cgtcgcgcag gccgatcgcc tcgtcggtga ggtcggcgcc gatcggggtg tcgggtggca
     3481 gcaccaggat gccggcgtgg tcgtcgaaca cgccgagctc gcgggcggag gcgagcatcc
     3541 cgtccgacca gcggccgcgg accttgcggc ggccgatctc caagccgccg gggagcttgg
     3601 ctccgggcgc ggcccagggg accacgtcgc cggggccgta gttgtgggcg ccgcagacca
     3661 cctcgcgtgg ctcggtgccc gggtcgatgg tgaccagggc gagccggtcg gcgtcggggt
     3721 ggcgggtgac gtcgaggacc cgggcggtga tgaccccggc gatgtcgtgc ccgacccagt
     3781 cgatggtctc gatctccagg ccggcgaagg tgagcttgtc ggccagtgcc tccggtccga
     3841 tgtcgccgag gtcgacgtgc tcggccagcc aggtgagcgg tacgcgcatg gggttcctcc
     3901 tttcggttct gttgcgctca gacgcccgct ttatgcgttg gccagccagc gcaggtcgtt
     3961 gtcggtgagg gtgcgcatgt cggccaggcc ggagcggagc atgtagggcc gctcgatgcc
     4021 catgccgaag gcgaaaccgg agaccctgtc ggggtcgtag ccaccggcgc gcagcacgtt
     4081 ggggtggacc atgccggcgc cgagcagctc gatccagccc tcgccgcggc aggtgcggca
     4141 gccggtgccg cggccgccgc agacaaagca ggacacgtcc acctccgcgc ccggctccac
     4201 gaatgggaag tacgagggcc gcaggcggat ctggcgctcc tcgccgaagt aggcgcgggc
     4261 gaaggcgagc agggtgccct tgaggtcgcc catggtgatg tgctcgtcca cggcgaggcc
     4321 ctcgatctgg ttgaagaccg gcacgtgagt ggcgtcgggg gtgtcggcgc ggtagcagcg
     4381 gccgggaatc accacgtaca gcggcggcgg ggcggcgagc atggagcgga tctgcatcgg
     4441 cgaggtatgg gtgcgcagca gcagcgggat cccctgcgcg tcgcgcgcct gcagcgacac
     4501 gctgtcctgc atggagcggg ccgggtggtc gggcgggaag ttcagcgcct cgaagttgta
     4561 ccagtcggtc tcgacctcgg g
//
LOCUS       DASPYY010000094         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2683650, whole genome shotgun sequence.
ACCESSION   DASPYY010000094 DASPYY010000000
VERSION     DASPYY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683650"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..821
                     /locus_tag="VEP73_01330"
     CDS             78..821
                     /locus_tag="VEP73_01330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HYT25115.1"
                     /translation="MRHALPAARRGTAAAVPPRAAGTDLPLAAGLAAAYAAFALTFRG
                     PRRSFWHRMTATGVTLGGLALAAEPGLRRTRFHGSDVAAGLASAAGLYGVFMVGDRLV
                     RRLLPSGGQEIGDIYALRDLRPRAELVARLGAVIGPAEELFWRGFVNERLVRRYGRWP
                     GAALGAAAYGGAHLATGNFTLFGAAGVAGAYWSALAAAGMPMGALVVSHVAWDLWAFL
                     VAPTSQRDARGHVAEQGSATTAAGGTPRR"
     gene            complement(790..894)
                     /locus_tag="VEP73_01335"
     CDS             complement(790..894)
                     /locus_tag="VEP73_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25116.1"
                     /translation="MTVIVPMHSRVRLGTLAQILEDAGVSVEEFRRLL"
     gene            complement(1007..1222)
                     /locus_tag="VEP73_01340"
     CDS             complement(1007..1222)
                     /locus_tag="VEP73_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYT25117.1"
                     /translation="MSERHELRLTAVVTPDEDWFMARCLEVPVTSQGPTPEAAVANLQ
                     EALELYLEDEPVPAPARVEYVDVCLSA"
BASE COUNT          174 a          582 c          531 g          227 t
ORIGIN      
        1 atcgacctcg agaccgccca gctccacgcg gccttcggct tcctgctggt ggccggcctg
       61 gccatcgcgg cggtccggtg aggcacgccc tgccggccgc ccgccgggga acggcagcgg
      121 ccgtcccgcc gagggccgcc gggaccgacc tgcccctggc ggccgggctc gcggccgcgt
      181 acgcggcctt cgcgctcacc ttccgcggcc cccggcggtc gttctggcat cgcatgaccg
      241 cgaccggggt cacgctcggc ggcctcgcgc tggccgccga gccggggctc aggcggaccc
      301 ggttccacgg gtccgacgtc gcggccgggc tcgcgtccgc ggccgggctg tacggcgtgt
      361 tcatggtcgg cgaccggctg gtccggcgcc tgctgccgag cggcggccag gagatcggcg
      421 acatctacgc gctgcgggac ctgcggcccc gggccgagct ggtcgcccgg ctcggcgccg
      481 tcatcgggcc cgccgaggag ctgttctggc gcgggttcgt caacgagcgg ctcgtgcgcc
      541 gctacgggcg ctggccgggc gccgccctcg gcgcggccgc ctacggcgga gcgcacctcg
      601 cgaccggcaa cttcaccctg ttcggggcgg cgggcgtggc cggcgcctac tggtcggcgc
      661 tggccgccgc cggcatgccg atgggcgccc tggtcgtgag ccacgtcgcc tgggacctct
      721 gggcgttcct cgtcgccccg acctcccagc gtgacgctcg cggtcacgtg gccgagcagg
      781 gctcggccac tacagcagcc ggcggaactc ctcgacgctg acgcccgcat cctcgaggat
      841 ctgcgcgagc gtgccgagac gcacgcgcga gtgcatcggc acgataaccg tcaccttcgt
      901 gtcctcgtgg cgcagctaca gtgcgaccca cgggtcgaga cctcgacata gccggcgcgg
      961 gcaagggcgc gcttcacgcg agccccggag acggcaggga ggctcgtcac gccgagaggc
     1021 acacgtcgac gtactcgacg cgcgcgggag ccggcaccgg ctcgtcctcc aggtacagct
     1081 cgagcgcctc ctgcaggttc gcgaccgccg cctcgggcgt cggcccctgg ctcgtgaccg
     1141 gcacctccag gcacctggcc atgaaccagt cctcgtccgg cgtgacgacc gccgtcaggc
     1201 gcagctcatg ccgctcgctc atcggcctcc ccggtcggag tcagggcgtc cccacggccg
     1261 agtgcgaccg ccaggcggta ccgttccgta ccagtgtacc tgtgcacgcg tacgctcctc
     1321 agcttctgcc tccgtacccg tgcggtcgtc ttccgccggt cggtgatccc tcacgccctg
     1381 gcgtgcttcc gtcgctccgc cgctggcgcg ttcttcacaa gatcgtacta gcggctttac
     1441 agtagatctc tactttaata gacagtccgt atgacagggc gtgagctcct ccggcggctc
     1501 aagcgacggg gctg
//
LOCUS       DASPYY010000095         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2263158, whole genome shotgun sequence.
ACCESSION   DASPYY010000095 DASPYY010000000
VERSION     DASPYY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263158"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VEP73_01345"
     CDS             <1..1589
                     /locus_tag="VEP73_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HYT25118.1"
                     /translation="GLASLHLHVSPFVMVPAGALAAALTAALLGVVVMRTRGHAFVII
                     TIAFLFLAQLVAVNAVGLTGGSNGLTLPLPSWDRDYQNFPFYYALLALLVLSIAMSAR
                     IRRSKLGAGLVAIREDEGKATTVGVNTRVYKVLGFAASAVLIGAAGGVFGYYLTFVDP
                     RGMFDILVSVQIVLAGLLGGRGTVWGPVLGAFLLEPVNEATNAWFGGSQLHLVIFGAL
                     LAAAVLFLPAGIVPTVTERLERRRAAAGAAAGLDRATRTTSTAVPPSAPAGAPRPDAD
                     LLELRGLAKRFGGLTAVDGCSFAVRAGSVTGLIGPNGSGKTTIFNLVSGTARPDRGEI
                     WFGGRRIDRAQPWERAWLGLGRTFQITRLLAQMTVLENVVAPLRRFSWRQLGADAVSG
                     PEARRAHELLEFVGMHEFAGERAGHLSFGQQKLVELAQVLMLEPRLILLDEPAGGVSP
                     RLVERISELIGELNRRGMTFLIVEHNMPMVLGLCDPVLVLSAGRCIAQGPPKAIQRDP
                     AVLDAYLGRDLAAPHQRLEV"
     gene            1589..2365
                     /locus_tag="VEP73_01350"
     CDS             1589..2365
                     /locus_tag="VEP73_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25119.1"
                     /translation="MAALLELTGVVAGYGGADVLQGVDLAVESGSITCIVGPNGAGKS
                     TVLRVVSGLLVPSSGRVELAGAAIGGLRPAEILARGVVQVPQQRGLFPSLTVRENVLM
                     GGYVIRGDRELVRRRHRQVEELVPVVRERAADRAGNLSGGQRRMVEFARSLMLDPAVV
                     LLDEPSLGLDPSSLRVVYDLIPVLRERGKTVLLVEQNVRIGLGVATHGVVMERGRVRA
                     AAPARDILADPATADLYLGGGVRDRPGPGATGRASRSSPA"
     gene            complement(2290..3174)
                     /gene="fdhD"
                     /locus_tag="VEP73_01355"
     CDS             complement(2290..3174)
                     /gene="fdhD"
                     /locus_tag="VEP73_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYT25120.1"
                     /translation="MTGRPGSKTRTRVLTVTGSEPHTRSDELATEEPLEIRVVSRTTA
                     TVAVTMRTPGADFELAAGFLYGEGVLTGRDDVTRIAYCTDPEVDAEQQYNVVSVGLAA
                     GALPDLRALERHFMTSSACGVCGKASLQALELRGCPVVPPGPVVDPAVLTSLPAALRE
                     AQGLFESTGGLHAAGLFTPDGGLVALREDVGRHNAVDKLVGWALLQGRLPLHDHLLMV
                     SGRSSYEILQKALAAGIPFVCAVSAPSSLAVALARRFGMTLVGFLRGERFNVYAGEER
                     LALPVAPGPGRSRTPPPR"
     gene            complement(3269..3805)
                     /locus_tag="VEP73_01360"
     CDS             complement(3269..3805)
                     /locus_tag="VEP73_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25121.1"
                     /translation="MHRGDQDPVDRERRKREVEEVRRSFTAVGRRLGTLGQQRRFSTV
                     NPVAVQPEARSAKADPAPPAPPAALELPTVQPVPRRPTWPLAAVVLGLIFAVGVLLGR
                     GIGDARPAPASRATPATITRTVTRSVVPPSCLTAVQRADEAIHLLVARVRDQRLSDSL
                     TSYAAESRACRKAAPEPG"
     gene            4294..5413
                     /gene="prfB"
                     /locus_tag="VEP73_01365"
     CDS             join(4294..4362,4364..5413)
                     /gene="prfB"
                     /locus_tag="VEP73_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824324.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HYT25122.1"
                     /translation="MPTDHAPELDALAKRLADAKEFLDLEAKERELGGLREQAAAPNL
                     WDDPGRARRVMSRMASVEADLAAAARLSGQLSDLETLNELALEEDDDSVAAELEQGLS
                     ELAAAVDELEVLVLLGGEFDERDAIVTIHPGAGGTESQDWAEMLWRMLTRWAERRGFK
                     VTVNDYQEGDEAGVKSVTMTLEGPRVYGLASAEAGTHRLVRISPFDQAKRRHTSFAAV
                     DVIPVLDDEDAPEIDDADLRIDIYRSSGPGGQGVNTTDSAVRITHLPTGIVVAVQNER
                     SQLANKTIALKILAARLAERARQEREAALAALRGDRGDIAFGNQIRSYVLHPYQMVKD
                     HRTDVETGNVQAVLDGDLDRFVAAELRRRAVGRLGDAR"
     gene            5597..>6141
                     /locus_tag="VEP73_01370"
     CDS             5597..>6141
                     /locus_tag="VEP73_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HYT25123.1"
                     /translation="MSAITGLYRRWRILVHELAKFGIVGVFNTFLDFGLANLLHFGFH
                     WHELAAKTGSTVVAATSSYFMNRHWTFRHRARTGLRREYSLFFLLNGVGLLISDACIL
                     VVQVGLHRTGVLWFNLAQVVGLAFGMLFRFTTYKRWVFVQPEVAAARAVAEGSVAEAS
                     VEGAPVGAPVLVPGDPRRNRS"
BASE COUNT          737 a         2139 c         2384 g          881 t
ORIGIN      
        1 ccgggctcgc ctccctgcac ctgcacgtgt cgcccttcgt catggtgccg gccggggcgc
       61 tcgccgccgc cctgaccgcc gccctgctcg gcgtcgtcgt gatgcgcacg cgcgggcacg
      121 cgttcgtgat catcaccatc gcattcctgt tcctggcgca gctcgtcgcc gtcaacgcgg
      181 tcgggctcac cgggggcagc aacgggctca ccctacccct gccctcgtgg gaccgggact
      241 accagaactt ccccttctac tacgcgttgc tcgcgttgct cgtcctctcc atcgccatgt
      301 cggcgcggat ccggcgcagc aagctgggcg cgggcctggt cgccatccgg gaggacgagg
      361 ggaaggccac caccgtcggg gtgaacaccc gggtctacaa ggtcctcggg ttcgccgcga
      421 gcgccgtgct gatcggcgcc gccggagggg tcttcggcta ctacctcacc ttcgtcgacc
      481 cgcgcgggat gttcgacatc ctggtgagcg tccagatcgt cctcgcgggc ctgctcggcg
      541 ggcgcgggac cgtctggggg ccggtcctgg gcgccttcct cctggagccg gtcaacgagg
      601 cgaccaacgc ctggttcggc ggctcccagc tgcacctggt gatcttcggc gcgctcctcg
      661 ccgccgccgt gctgttcctg ccggccggga tcgtcccgac cgtgacggag cggctcgagc
      721 ggcgccgcgc ggccgcgggg gccgccgccg gcctcgaccg ggcgacgcgc acgaccagca
      781 cggccgtccc gccctcggcg ccggcgggcg cgcctcggcc agacgccgac ctgctggagc
      841 tgcgcgggct ggccaagcgc ttcggcggcc tgaccgcggt cgacggctgc tccttcgcgg
      901 tccgggccgg ctccgtcacc gggctgatcg ggcccaacgg ctcgggcaag accacgatct
      961 tcaacctcgt cagcgggacg gccaggccgg acaggggtga gatctggttc ggcgggcggc
     1021 ggatcgaccg cgcccagccg tgggagcgcg cctggctcgg cctgggccgc acgttccaga
     1081 tcacgcggct gctggcgcag atgaccgtgc tcgagaacgt ggtggccccg ctccggcggt
     1141 tctcgtggcg gcagctcggc gccgacgcgg tgagcgggcc ggaggcgcgg cgcgcccacg
     1201 agctgctcga gttcgtcggc atgcacgagt tcgccggcga gcgggccggc cacctgtcgt
     1261 tcgggcagca gaagctggtc gagctcgccc aggtgctgat gctcgagccg aggctgatcc
     1321 tgctcgacga gccggcgggc ggggtcagcc cgcggctggt ggagcgcatc tccgagctga
     1381 tcggtgagct caaccgccgc ggcatgacct tcctgatcgt cgagcacaac atgcccatgg
     1441 tcctcgggct gtgcgacccg gtgctggtcc tgagcgccgg ccgctgcatc gcccaggggc
     1501 cgcccaaggc gatccagcgc gatcccgccg tgctcgacgc ctacctgggc cgggacctcg
     1561 ccgcgccgca ccagcggctg gaggtctgat ggcggcgctg ctcgagctca ccggcgtggt
     1621 ggcgggctac ggcggggccg acgtgctcca gggcgtcgac ctcgcggtcg agtccggctc
     1681 gatcacctgc atcgtgggac ccaacggcgc cggcaagtcg accgtgctgc gggtggtgag
     1741 cgggctgctc gtgccgtcga gcggccgggt cgagctcgcg ggcgccgcga tcggcggcct
     1801 ccgcccggcg gagatcctcg cccgcggcgt cgtccaggtc ccccagcagc gggggctctt
     1861 cccgagcctc accgtcaggg agaacgtcct gatggggggc tacgtgatcc gcggggaccg
     1921 ggagctggtg cggcgtcgcc accgccaggt cgaggagctc gtgccggtcg tgcgggagcg
     1981 cgccgcggac cgggccggga acctgtccgg cggccagcgg cgcatggtcg agttcgcccg
     2041 ctcgctgatg ctcgacccgg ccgtcgtgct gctcgacgag ccgtcgctcg gcctggaccc
     2101 gagcagcctg cgggtcgtct acgacctcat cccggtcctg cgtgagcggg gcaagaccgt
     2161 cctgctggtg gagcagaacg tgcgcatcgg cctgggcgtg gccacgcacg gggtggtcat
     2221 ggagcgtggg cgcgtccggg ccgccgctcc ggcaagagac atcctggccg acccggccac
     2281 cgccgacctc tacctcggcg gcggcgtccg cgaccgtccg ggcccggggg cgacgggcag
     2341 ggcgagccgc tcctcgccgg cgtagacgtt gaagcgctcg ccgcgcagga agccgacgag
     2401 cgtcatgccg aaccgccgcg cgagggcgac cgcgaggctc gacggggcgg acacggcgca
     2461 gacgaacggg atgccggcgg cgagggcctt ctgcaggatc tcgtagctcg accggccgct
     2521 gaccatgagc aggtggtcgt ggaggggcag acggccctgg aggagggccc agccgacgag
     2581 cttgtcgacc gcgttgtgcc ggccgacgtc ctcgcgcagg gcgaccagcc cgccgtcggg
     2641 ggtgaacagc ccggccgcgt ggaggccgcc ggtggactcg aacaggccct gtgcctcgcg
     2701 gagcgcggcg gggaggctgg tcagcacggc ggggtcgacg accgggccgg gggggacgac
     2761 cgggcagccg cggagctcga gcgcctgcag gctcgccttg ccgcagacgc cgcaggcgct
     2821 ggacgtcatg aagtggcgct cgagcgcccg caggtcgggg agcgcacccg cggcgagccc
     2881 gacgctgacg acgttgtact gctgctcggc gtccacctcg gggtcggtgc agtaggcgat
     2941 ccgcgtgacg tcgtcgcgcc cggtgagcac gccctcgccg tagaggaacc cggcggcgag
     3001 ctcgaagtcg gcgccggggg tgcgcatggt gacggcgacg gtcgcggtcg tgcggctcac
     3061 gacccggatc tcgaggggct cctcggtggc gagctcgtcg gaccgggtgt gcggctcgct
     3121 gccggtgacg gtgaggacgc gcgtcctcgt cttgctgccc ggtcgcccgg tcacgccagc
     3181 tccttccccg ccccgttcgg ctccaggggt cgaccaggcg ccggcccgcc cggtcctccc
     3241 cattgaagca ggccgctgat cgctcgtgct atccgggttc cggggccgcc ttgcggcagg
     3301 cccggctctc ggccgcgtag gacgtcaggc tgtcgctgag gcgctggtcc ctgaccctcg
     3361 cgacgagcag gtggatggcc tcgtcggcgc gttgcacggc ggtgaggcag gacgggggga
     3421 cgacgctcct ggtgaccgtc cgggtgatgg tcgccggcgt cgcccgggac gcgggggcgg
     3481 gccgtgcgtc gccgatgccg cggccgagga ggacgcccac cgcgaagatg aggccgagga
     3541 cgacggcggc aaggggccac gtcgggcggc gggggacggg ttggacggtg gggagctcga
     3601 gcgctgccgg cggggcgggc ggggcggggt cggccttggc gctccgggcc tccggctgga
     3661 cggccacggg gttgacggtg gagaagcgcc gctgctgccc gagcgtgcca aggcgccgcc
     3721 cgaccgcggt gaaggaccgg cggacctcct cgacctcccg cttgcgccgt tcgcggtcga
     3781 cggggtcctg gtcgccccgg tgcatggccg tctcgtcctc gagcctcgcc gcctcggcgg
     3841 ggtcgggcgt cgtggggcgt ggtgccgccg ccgggacggg gcgctccgtg gacgccgagg
     3901 gatcgggccg tcggcggctc gagccgtcgc gttcgactgg ttcgttgtcg actggttcgt
     3961 tgtgtgctgc ggtcatgggc gctggtggcg gctcctggcc tctcgggatc cgggtcctgc
     4021 gggggcgctg acggcggcga gggccgcgtg gcgttgccgc cgtcagctcg ccccgtcata
     4081 acaggttgat ccggcaacga ccaggttcgg cgcggtcgac tcggtaggag tacgtagctg
     4141 ctggtccgtt cgccgtttgc cgggggtcga tgccgttttc ggaacgattc gcgcgacggc
     4201 cggtggccgc cgcgcgcggc gaggtgagtg ccgggcgacg gcgggtacga tggtgggaac
     4261 gtcccgccgc ctgacgagga gatgccgcac gccatgccga ccgaccacgc tcccgagctc
     4321 gacgccctgg ccaagcgcct ggccgacgcg aaggagtttc tttgacctcg aggccaagga
     4381 gcgggagctc ggcggcctgc gggagcaggc ggcggcgccg aacctgtggg acgaccccgg
     4441 ccgggcccgg agggtgatga gccggatggc gtcggtggag gcggacctcg ccgccgccgc
     4501 gcggctgtcc gggcagctgt cggacctcga gaccctgaac gagctggcgc tcgaggagga
     4561 cgacgactcg gtcgccgccg agctcgagca gggcctgtcg gagctggccg ccgcggtcga
     4621 cgagctcgag gtgctcgtgc tgctgggcgg ggagttcgac gagcgcgacg cgatcgtgac
     4681 catccatccg ggggcgggcg ggaccgagtc gcaggactgg gccgagatgc tgtggcggat
     4741 gctgacccgc tgggcggagc ggcgcgggtt caaggtgacg gtgaacgact accaggaggg
     4801 cgacgaggcc ggcgtcaagt cggtgacgat gaccctggag ggccctcggg tgtacgggct
     4861 ggcgtcggcg gaggccggca cccaccggct ggtgcggatc tcaccgttcg accaggccaa
     4921 gcgcaggcac acgtcgttcg ccgcggtcga cgtgatcccg gtgctcgacg acgaggacgc
     4981 gcccgagatc gacgacgcgg acctgcggat cgacatctac cgctcgtcgg gaccgggcgg
     5041 ccagggcgtg aacaccaccg actcggcggt gcgcatcacc cacctgccga ccgggatcgt
     5101 cgtcgcggtg cagaacgagc gctcgcagct cgcgaacaag acgatcgcgt tgaagatcct
     5161 ggccgcgcgg ctggccgagc gggcccgcca ggagcgcgag gcggccctgg cggcgttgcg
     5221 cggcgaccgc ggcgacatcg ccttcggcaa ccagatccga tcgtacgtcc tgcacccgta
     5281 ccagatggtc aaggaccacc ggaccgacgt ggagacgggc aacgtgcagg cggtgctcga
     5341 cggcgacctc gaccggttcg tcgccgccga gctgcggcgc cgcgcggtcg gcaggcttgg
     5401 cgatgcccga taggcgatgc cggatagacg gggggccgat gcgggatctt gaccgacggt
     5461 gcgctaggct tcccgggccg ttcccgtctc actggagtgc catgtcctct cccactccca
     5521 cgatcccgac ctccgcggtg cgcgcccctg cccgcgcgcc gctcgacccg gccgccccca
     5581 gggcggggat gcgcggatga gcgcgatcac ggggctctac cggcggtggc gcatcctggt
     5641 ccacgagctg gcgaagttcg gcatcgtcgg cgtgttcaac accttcctgg acttcgggct
     5701 ggccaacctg ctgcacttcg ggttccactg gcacgagctg gcggccaaga ccgggtcgac
     5761 ggtggtggcg gcgacgtcgt cgtacttcat gaaccggcac tggacgttcc ggcaccgggc
     5821 caggaccggc ctgcgccggg agtattccct gttcttcctg ctcaacggcg tggggctctt
     5881 gatctccgac gcctgcatcc tggtcgtgca ggtcggcctg cacaggaccg gcgtgctgtg
     5941 gttcaacctg gcccaggtgg tcggcctggc cttcggcatg ctgttccggt tcaccaccta
     6001 caagcgctgg gtgttcgtgc agccggaggt ggcggcagcg cgcgcggtgg ccgagggctc
     6061 ggtggccgag gcctcggtcg agggcgcgcc ggtgggcgcg ccggtgctcg tgcccggcga
     6121 cccccgccgc aaccgctcgt g
//
LOCUS       DASPYY010000096         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_156158, whole genome shotgun sequence.
ACCESSION   DASPYY010000096 DASPYY010000000
VERSION     DASPYY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156158"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VEP73_01375"
     CDS             complement(<1..1076)
                     /locus_tag="VEP73_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25124.1"
                     /translation="MASPDESRADDARDPSSAPESPEASLDAESDGGLRTGAVAPGRP
                     RGAASFPGGPPPVSAPPPEEPAPPPAPVAVQSPPRFQVTTVESTAAASFPGGPPPVST
                     PPVSAPPVSAPPPTSTSPGEESAPPSAPAGEPPAPRAAGERPAVGDVPGPGGAGGNGR
                     ASRGGRASRASGIEASRASGIEASRASGSVASRASGVGGGAWAAEDGGAARDTAEAEG
                     TAEPPVAEAVEAAGAVDAAGAVDADAPPARAGGRRRRRGRRSAQAERSDALAEELPAP
                     AGPPARAGEPDPLDGPVALAAPAAAEPYGDEQAPGDAGVHRDEDAEEDQEGPGSARTR
                     ARRRRRAAAKARVNSEPASAEDAPP"
     gene            complement(1503..>2522)
                     /gene="rodA"
                     /locus_tag="VEP73_01380"
     CDS             complement(1503..>2522)
                     /gene="rodA"
                     /locus_tag="VEP73_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYT25125.1"
                     /translation="ASLREQGLPESLVMRRQLLSLAVGLAGMVAVTLVDYRRLQAYAG
                     AVLGAVVLALAAVLTPLGATANGAQSWFGFGSYQVQPSELAKVGVIVALAAAVGGRAG
                     SERSEWRRLLLALGVVGLVSVELLLQPDFGTFMVFVAVLLGVLLVGGVPLRWLLVLVL
                     VGSLGVAGMFRLNLLKEYQKERLTAFVNPSADTGGRGYTYNYRQALIAIGSGGLTGKG
                     YLRGTQTNLSYVPEQRTDFIFTVIGEELGFAGGAVLLLLFAVLLWRGLRIAALARDPF
                     GCLLATGAVTMFAFQAFVNIGMTIGIMPITGIPLPFVSYGGSSLISSFLAVGLLENVH
                     MRRYL"
BASE COUNT          316 a          941 c          997 g          268 t
ORIGIN      
        1 gggggggcgt cctcggcgga ggccggctcg gaattgaccc tggccttggc ggcggcgcgg
       61 cggcggcggc gggcgcgggt gcgggccgag ccggggccct cctggtcctc ctcggcgtcc
      121 tcgtcgcgat ggacccccgc atcgcccggt gcctgctcgt cgccgtacgg ctcggccgcg
      181 gcgggcgccg cgagggcgac cggcccgtcc agcgggtccg gctcgccggc ccgggcgggc
      241 gggccggcgg gcgccgggag ctcctcggcg agcgcgtcgg aacgctcggc ctgcgccgag
      301 cggcggcctc ggcggcgccg ccgcccgccc gcccgggccg gcggggcgtc cgcatccacg
      361 gccccggcgg cgtccacggc cccggcggcc tccacggcct cggcgaccgg tggctcggcg
      421 gtcccttcgg cctcggcggt gtccctggcg gccccgccgt cctccgccgc ccaggcgccg
      481 cccccgacgc ctgaggcgcg cgaggccacg ctgcctgaag cgcgggaggc ctcgatgcct
      541 gaagcgcgcg aggcctcgat gcctgaagcg cgcgaggccc ggccgcccct ggaggcgcgc
      601 ccgttcccgc ccgcaccgcc cgggccgggg acgtcgccca cagcgggccg ctcccccgcc
      661 gcccgaggag ccggcggttc cccggcgggc gcggacgggg gggcggactc ctcgcccggc
      721 gaagtgctcg tgggtggcgg ggcgctcacc ggcggggcgc tcaccggcgg ggtgctcacc
      781 ggtggcgggc cgccagggaa ggaggcggcc gcggtgctct ccaccgtggt cacctggaag
      841 cgcgggggcg actggaccgc cacgggggcc gggggcgggg ccggctcctc gggcggcggg
      901 gcgctcacgg gcggcgggcc gccagggaag gaggcggccc ctcggggccg gccgggcgcg
      961 acggcgcccg tgcggagccc gccgtcggac tcggcgtcca gggatgcctc cggggattcg
     1021 ggtgcgctgc tggggtcccg ggcgtcgtct gcccgggact cgtcggggga agccattgat
     1081 gctcctcacc aagctctggc ggccaccgac gggggcggcc gaaaccgttg cgcgctcggg
     1141 ggagcctccc ccggccgagc cgggcccggc ccgtgaccgt cggtaccggt ccgagccgtc
     1201 cgcccggtcg ggcggcgccg ccacaccgtc cggatggtcc caggctgggt gagcggcagc
     1261 ccatccagtg ggaacgaccg gcccgtcgtc tgcgacgaag ccgcgagccc cttgccggcg
     1321 cctcgaccct cgggcaggtc ccgatccccg ccggccggcg gccggagcgg gccccgagat
     1381 gcccaggaac tgagtgaggc ggcggcacga cgtcgggccg caccgcaggg tggtatggcg
     1441 aaagacctca cgagtctgcc agaaggctcc ggtcgcggca aaggttcgtt cgttccgacc
     1501 ggtcagaggt agcggcgcat gtggacgttc tcgagcagcc cgacggcgag gaagctcgag
     1561 atcagcgacg agcccccgta ggagacgaac gggagcggga tcccagtgat cggcatgatc
     1621 ccgatggtca tgccgatgtt gacgaacgcc tggaaggcga acatcgtgac ggcgccggtc
     1681 gcgagcaggc agccgaaggg gtctctggcc agcgcggcga tgcgcaggcc gcgccacagc
     1741 agcacggcga acagcagcag cagcacggct ccgccggcga agcccagctc ctccccgatg
     1801 accgtgaaga tgaagtcggt gcgctgctcg ggcacgtagc tcaggttcgt ctgggtgccg
     1861 cgcaggtagc ccttgccggt gagcccgccc gacccgatcg cgatgagcgc ctgccggtag
     1921 ttgtaggtgt agccgcggcc gcccgtgtcc gcgctcgggt tcacgaacgc ggtcaggcgc
     1981 tccttctggt actccttgag caggttgagc cggaacatgc cggccacccc gaggctgccg
     2041 acgagcacga gcacgagcag ccagcgcagc gggacgccgc cgaccagcag cacgccgagc
     2101 agcacggcca cgaacaccat gaacgtgccg aagtcgggct ggaggaggag ctcgacgctg
     2161 accaggccga ccacgccgag cgcgagcagg aggcggcgcc actccgaccg ctcggacccc
     2221 gcccgcccgc cgaccgcggc ggcgagggcg acgatcacgc cgaccttggc gagctcggac
     2281 ggctgcacct ggtaggagcc gaagccgaac caggactgcg ccccgttggc ggtggcgccg
     2341 agcggggtga gcacggcggc gagggcgagc accaccgccc cgagcaccgc gcccgcgtac
     2401 gcctggaggc ggcggtagtc gacgagcgtc accgcgacca tgccggcgag ccccacggcg
     2461 aggctcaaga gctgcctccg catgaccagg ctctcgggca ggccctgctc gcgcaggctc
     2521 gc
//
LOCUS       DASPYY010000097         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_859122, whole genome shotgun sequence.
ACCESSION   DASPYY010000097 DASPYY010000000
VERSION     DASPYY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859122"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VEP73_01385"
     CDS             <1..509
                     /locus_tag="VEP73_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT25126.1"
                     /translation="CHSATTTPLVLAFPQLSGYAAADFFMGKELRDKGVVHRDIKPPN
                     VMLTRTGAKLLDFNIASRVGDPVDTQSGTPPYQAPDADLTRWDVSTDLFAVGVMLYEL
                     LCNGQHPYPGGKPMVGEGIIDPKTIRPELDDALVRFLRKACAPERGRRFSTAAEMAAE
                     LREIRATL"
     gene            complement(585..1004)
                     /locus_tag="VEP73_01390"
     CDS             complement(585..1004)
                     /locus_tag="VEP73_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25127.1"
                     /translation="MPPAPAQAPRVLLVDDSAELRQALRRALEDEGIEVVGEAADGAA
                     GVAQAKALEPDVVLMDLRMPVMDGVAATSAIKQALPVTQVLILTAYEEWGSSARAAGA
                     YAYLVKGTGPELLRDVVLQAWKLKVGLEARLDGGNPG"
     gene            complement(1175..2131)
                     /locus_tag="VEP73_01395"
     CDS             complement(1175..2131)
                     /locus_tag="VEP73_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT25128.1"
                     /translation="MSGAEPLRLLGVDIGGTKIAVAVVDPASGAVIARRAVSTPAQEG
                     PVAVVEAAADLAARVLVAYPATAAGVGAPGVVDARRGVVATSTGVLPGWEGTAVAAEL
                     ERRLGLPFAADNDVNAAALGEARYGAGRGLDSFLLVVVGTGLGGAIVQGGRVLRGATG
                     TTGEIGHVPVFGVDGVVCSCGSSGHLEAVVAAPAITAAYHRATGEPGVDIRVIARRAT
                     AGEAEAVATLAAVGTVFGRTLGGLVNTLDPEAVVLGGGVMQAGPPIWEPLEAALRAEV
                     LPSVSGVPLRPAALGVDAGVIGAAALALELVEAPYAAAGREP"
BASE COUNT          284 a          832 c          774 g          261 t
ORIGIN      
        1 ggtgtcattc agccacaacc acgccgctcg tcctagcatt cccgcagctc agcggctacg
       61 ccgccgccga cttcttcatg gggaaggagc tacgggacaa gggtgtggtg catcgtgaca
      121 tcaagccccc gaacgtcatg ctcacgcgga cgggggcgaa gctgctcgac ttcaacatcg
      181 cctctcgggt cggtgacccg gtggacacgc agtcgggcac gccgccgtac caggcgcccg
      241 atgccgacct cactcgatgg gacgtctcca ccgacctgtt cgccgtcggc gtgatgctct
      301 acgagctgct ctgcaacggc cagcacccgt accccggcgg gaagccaatg gtgggtgagg
      361 ggatcatcga tccgaagacg atccgtcctg agctcgatga cgcgctcgtg aggttcctgc
      421 gcaaggcttg tgctcccgag cgcggcaggc gcttctccac cgccgctgag atggcagccg
      481 aactccgcga gatccgggct acgttgtgac gtgggggctc cgcacggcat cgcccgggag
      541 atccacccgg ccacgcgccg agcatcctcg ccgccacgac ccggtcagcc agggttcccg
      601 ccgtccaggc gcgcctcgag gccgaccttg agcttccagg cctgcaggac cacgtcgcgg
      661 agcagttcgg gcccggtccc cttgacgagg taggcgtagg ccccggcggc gcgcgcgctc
      721 gagccccact cctcgtaggc ggtcaggatc aggacctggg tgaccggcag cgcctgcttg
      781 atcgcgctgg tggccgcgac gccgtccatg accggcatgc gcaggtccat gagcaccacg
      841 tcgggttcga gcgccttggc ctgcgccacc ccggcggcgc cgtcggccgc ctcgccgacg
      901 acctcgatgc cctcgtcctc gagcgcccgg cgcagcgcct ggcgcagctc ggcactgtcg
      961 tcgacgagca gtacccgggg cgcctgggcc ggggccggag gcaccccgct caccaggcgc
     1021 cggcgacgcc gagggagaaa ggtgggaccc gctgcttcat gtcctcccca tcggcaggat
     1081 cggttgccca cccagtcctt cggcaccccg gcgggcaggg ttgacccgct ccgcgcacgg
     1141 taccagaccg ggggacggcc ggtcgcggga cgggtcaggg ctctctcccg gcggccgcgt
     1201 acggtgcctc caccagctcg agggcgagcg cggcggcccc gatcacgccg gcgtcgacgc
     1261 cgagggcggc cggccggagc ggcaccccgg acaccgacgg caggacctcg gcgcgcaggg
     1321 cggcctcgag cggctcccag atcggcgggc cggcctgcat gaccccgccg ccgagcacga
     1381 ccgcttccgg gtcgagcgtg ttgaccaggc cgccgagcgt gcggccgaag acggtcccga
     1441 cggcggcgag cgtggcgacc gcctcggcct cgccggccgt ggcgcgccgg gcgatcaccc
     1501 ggatgtccac cccaggctcg cccgtggccc ggtggtacgc ggcggtgatg gccggggccg
     1561 cgacgaccgc ctcgaggtgg ccggagctgc cgcagctgca caccacgccg tcgacgccga
     1621 acaccgggac gtggccgatc tccccggtcg tgccggtcgc gccgcgcagc acccggccgc
     1681 cctgcacgat ggcgccgccg agcccggtcc cgacgacgac gagcaggaac gagtcgagcc
     1741 cgcggccggc gccgtacctc gcctcgccga gcgccgccgc gttgacgtcg ttgtcggccg
     1801 cgaacggcag gccgagccgg cgctccagct cggcggcgac cgcggtgccc tcccagccgg
     1861 gcaggacgcc ggtcgaggtg gcgacgacgc cgcggcgcgc gtcgaccacg ccgggggcgc
     1921 cgacgccggc ggccgtggcc gggtaggcga cgagcaccct ggcggcgagg tcggcggccg
     1981 cctccaccac cgcgacgggt ccctcctggg ccggcgtgga gaccgcgcgc ctggcgatca
     2041 cggcgccgct ggccgggtcg accacggcga ccgcgatctt ggtgccgccg atgtccaccc
     2101 cgagcagccg caggggctcc gccccgctca cgggaccggc cctcccggcc t
//
LOCUS       DASPYY010000098         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2263741, whole genome shotgun sequence.
ACCESSION   DASPYY010000098 DASPYY010000000
VERSION     DASPYY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263741"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VEP73_01400"
     CDS             complement(<1..602)
                     /locus_tag="VEP73_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HYT25129.1"
                     /translation="MTAGGRVRPERGWSLVYEGFEPAREGLREALCTLGNGFFATRGA
                     VPEARADGVHYPGTYVAGLYNRLTTELAGRGVENEELVNVPNWLPLEFRAAGGPWFAV
                     FEADLLGHRHELDLRAGLLLRELRWRDGRGRVTRCTQRRFVHMADPHLAGLETTFVAE
                     NWSGTLEVRSGLDGRVVNAGVARYRDLANRHLDVLGAGPAG"
     gene            complement(599..1375)
                     /locus_tag="VEP73_01405"
     CDS             complement(599..1375)
                     /locus_tag="VEP73_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYT25130.1"
                     /translation="MAFGGAIFDVDGVLVDSPHERAWEESLRELMEHEWRDVRPGTSW
                     APDRFTTELYQQVMAGKPRFEGARAVLEHFGVPDLDRRTREYGDRKQQRVVELIEAGE
                     FHAFADALRFLLDVKRLGIRVASASSSKNAALFLEQIRLDTFAEREGIAGVPPGLTLL
                     DAFDADVTGRDFPRGKPDPAIFLAAAEELGLPPAACFVVEDAASGVQAAKAGGMAALG
                     VARLHDEDLLRDAGADLVVTTLDEVDRDALAAGRLERRPR"
     gene            complement(1401..2489)
                     /locus_tag="VEP73_01410"
     CDS             complement(1401..2489)
                     /locus_tag="VEP73_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYT25131.1"
                     /translation="MNGPPPTGTVHVTVATPLEQELAGRIAATGPRVEVAYDPALLPP
                     ARYPSDHKGDPGFRRGPAGERRWRELLAGAEVLFGVPGDSAEGLAAAVRGLPRLRWVQ
                     ATSAGAGQQVRAAGLARADLERVAVTSASGVHAGPLAEFCLFGLLAFAKGWRRLERDR
                     DRRRWDHYAVSELRDRTALIVGLGHVGAEVARLARCFGMRTVAVKRHPDRSPARRRGS
                     ADDRVDELHPTSRLHDLVGGADAVVVTLPLTRETEGLVDRAAIARMRPAAVLVNVGRG
                     GVVDEAALVEALEAGRIGGAALDVTATEPPPASSPLWHLDNVLLSPHTMALVERENER
                     IVALFVANLRRWLAGEPLEHRVDPDLYY"
     gene            2729..>3629
                     /locus_tag="VEP73_01415"
     CDS             2729..>3629
                     /locus_tag="VEP73_01415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYT25132.1"
                     /translation="MRRVLVGVTILVTLPCVVALACAPPAAADSLAGARARERALRTQ
                     LQAAAARLDAANDALEAVEQRLAFDQRQVAQAGSRLAAARASLDGQVASIYRSGGMAM
                     VGAILGNSTNQMPERVELLTVLLDRQSEGVAEADAASESYRFALARLRDDRAQARAAH
                     GGAAAALAQLNGKLKTASALTTRLAGFPGGQSSYPPPVLGGIACPVEPPYTFTDTYGA
                     PRPGGRTHKGTDVMAPYGAREFAYTAGVISDEHANALGGITLYLKGDDGNEYYYAHLS
                     RYAVARGARVVAGQLVGYVGNTGD"
BASE COUNT          455 a         1408 c         1352 g          414 t
ORIGIN      
        1 ccggcgggcc cggccccgag cacgtcaagg tgccggttcg cgaggtcgcg gtagcgggcc
       61 acgccggcgt tgaccacccg gccgtcgagc ccggaccgca cctcgagcgt ccccgaccag
      121 ttctccgcca cgaaggtcgt ctccaggccg gcgaggtgcg ggtctgccat gtgcacgaag
      181 cggcgctgcg tgcagcgtgt cacgcgcccc cgcccgtcgc gccagcgcag ctcgcggagg
      241 agcagcccgg cgcgcaggtc gagctcgtgg cggtggccga gcaggtcggc ctcgaagacc
      301 gcgaaccacg gcccgccggc ggcccggaac tcgagcggca gccagttggg cacgttgacc
      361 agttcctcgt tctcgacccc gcggccggcc agctcggtgg tgagccggtt gtacaggccg
      421 gcgacgtagg tgcccgggta gtggacgccg tcggcccgcg cctcggggac cgcgccgcgg
      481 gtcgcgaaga agccgttgcc gagcgtgcac agggcctcgc ggagcccctc gcgcgcgggc
      541 tcgaacccct cgtacacgag cgaccagccg cgttcgggcc gcacccgccc gcccgccgtc
      601 atcgcggcct ccgctcgagc cggccggcgg ccagcgcgtc ccggtcgacc tcgtccagcg
      661 tcgtgacgac caggtcggct cccgcgtcgc ggagcaggtc ctcgtcgtgc agccgggcga
      721 cgccgagcgc cgccatgccg cccgccttgg ccgcctgcac gccggaggcg gcgtcctcga
      781 ccacgaagca ggccgcgggc ggcagcccga gctcctccgc ggcggccagg aagatcgccg
      841 ggtccggctt gccgcgcggg aagtcccgcc cggtgacgtc ggcatcgaac gcgtcgagca
      901 gggtgaggcc gggcgggacg ccggcgatgc cctcacgctc cgcgaacgtg tccaggcgga
      961 tctgctcgag gaacagcgcc gcgttcttcg acgacgaggc ggacgccacc cggatgccga
     1021 gccgcttcac gtcgaggagg aagcgcagcg cgtccgcgaa cgcgtggaac tcgcccgcct
     1081 cgatcagctc cacgacccgc tgctgcttgc ggtcgccgta ctcccgggtg cggcggtcga
     1141 ggtcggggac gccgaagtgc tcgaggacgg ccctggcgcc ctcgaagcgc ggcttgccgg
     1201 ccatcacctg ctggtagagc tcggtggtga agcggtcggg cgcccagctg gtgccgggcc
     1261 ggacgtcccg ccactcgtgc tccatcagct cccgcagcga ctcctcccag gcccgctcgt
     1321 ggggggagtc gacgagcacc ccgtcgacgt cgaagatcgc gccaccgaag gccacggccg
     1381 tcacgtccct ttcctggtcg tcagtagtag aggtccgggt cgacgcggtg ctcgagcggc
     1441 tccccggcca gccagcggcg caggttggcc acgaacagcg cgacgattcg ctcgttctcc
     1501 cgctcgacca gcgccatcgt gtgcgggctg aggagcacgt tgtccaggtg ccagagcggg
     1561 ctcgacgccg ggggcggctc cgtcgcggtc acgtcgagcg ccgcgccgcc gatccggccg
     1621 gcctcgagcg cctcgacgag cgccgcctcg tcgaccaccc cgccgcgccc gacgttgacc
     1681 aggaccgcgg ccgggcgcat ccgggcgatc gccgcccggt cgaccaggcc ctcggtctcg
     1741 cgggtgagcg gcagggtgac gaccaccgcg tcggctccgc cgacgaggtc gtggagccgg
     1801 ctcgtggggt gcagctcgtc gacgcggtcg tcggcggacc cgcgccgccg ggcgggagaa
     1861 cggtcggggt ggcgcttcac ggcgaccgtg cgcatcccga agcaccgcgc aagcctggca
     1921 acctcggcgc cgacgtggcc cagcccgacg atcagcgcgg tgcggtcccg cagctccgag
     1981 acggcgtagt ggtcccagcg ccggcggtcc cggtcgcgct cgagccggcg ccagcccttg
     2041 gcgaacgcga gcagcccgaa caggcagaac tcggccagcg gcccggcgtg cacgccgctc
     2101 gccgacgtga cggccacccg ctcgaggtcg gcccgcgcca ggccggccgc ccgcacctgc
     2161 tggccggcgc ccgcgctggt cgcctggacc cagcgcagcc gcgggaggcc ccgcacggcc
     2221 gcggccagcc cttcggccga gtcgcccggg acgccgaaca gcacctcggc gccggccagg
     2281 agctcgcgcc agcgccgctc gcccgcgggg ccgcgccgga agccggggtc gcccttgtgg
     2341 tcggacgggt acctggccgg cggcagcagc gccgggtcgt aggccacctc gacccggggg
     2401 ccggtggccg cgatccgccc ggccagctcc tgctcgagcg gtgtcgcgac cgtgacgtgg
     2461 accgtcccgg tgggcggcgg gccgttcatc gggggatcct ccttgccgga gctcggcgcc
     2521 cgtcgcggcc gcgcgggacc tggcgggccg gccatgccgg ccacggcaga cccccacgca
     2581 aaggatagcc gccgaggagc ggcacgagcg acacgaagga gggtcccggg gcccggtgcg
     2641 attggcgtgc cgcgggggcc gcggtgtagg ttgcaggccc cctgagggct tccccgcctc
     2701 cgttcccccg acggaaaggc tgccgcagat gcgccgggtg ctcgtcggcg tgacgatcct
     2761 cgtgacactc ccgtgcgtcg tggcactcgc atgcgcgccg ccggcggccg ccgacagcct
     2821 ggcgggcgcc cgcgcccgcg agcgcgcgct gcgcacccag ctccaggccg cggctgcccg
     2881 gctcgacgcg gccaacgacg cgctcgaggc ggtcgagcag cgcctggcgt tcgaccagcg
     2941 gcaggtcgcg caggccgggt cccggctcgc cgccgcccgc gcctcgctcg acggccaggt
     3001 cgcgtccatc taccgctcgg gcggcatggc gatggtcggc gcgatcctcg gcaactcgac
     3061 gaaccagatg cccgagcggg tggagctgct caccgtcctc ctcgaccggc agagcgaagg
     3121 ggtcgcggag gcggacgcgg cgagcgagtc gtaccggttc gcgctcgcca ggctgcggga
     3181 cgaccgcgcg caggcgcggg cggcgcacgg cggcgccgcc gcggccctgg cgcagctgaa
     3241 cggcaagctg aagacggcga gcgcgctcac cacccggctc gccggcttcc ccggcggcca
     3301 gtcgagctac ccgccgcccg tgctcggcgg gatcgcctgc ccggtggagc cgccctacac
     3361 cttcaccgac acctacgggg cgccccgccc cggcgggcgg acccacaagg gcaccgacgt
     3421 catggccccc tacggcgcca gggagttcgc ctacacggcc ggcgtgatct ccgacgagca
     3481 cgccaacgcc ctcggcggga tcacgctgta cctgaagggg gacgacggca acgagtacta
     3541 ctacgcccac ctgtccaggt acgccgtggc ccgaggggcg cgcgtcgtgg ccggccagct
     3601 cgtcggctac gtcggcaaca ccggcgacg
//
LOCUS       DASPYY010000099         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2122900, whole genome shotgun sequence.
ACCESSION   DASPYY010000099 DASPYY010000000
VERSION     DASPYY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122900"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VEP73_01420"
     CDS             complement(<1..324)
                     /locus_tag="VEP73_01420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYT25133.1"
                     /translation="MADTHATLKTATLKTSKGDIRVALFPDTAPRTVENFVGLATGTR
                     TWKDPRTGAEQRRPLYDGTIFHRVIPQFMVQGGDPLGTGTGGPGYKFADEVAGPRRFD
                     RPGYLA"
     gene            complement(391..1191)
                     /locus_tag="VEP73_01425"
     CDS             complement(391..1191)
                     /locus_tag="VEP73_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HYT25134.1"
                     /translation="MVVFDDVEVALLDLDGTVYVGTRPVPGAGEAVERLRRDGLAVRF
                     TSNTDSTSPAALAARLAGMGIPAAEDDFLTPVVLARRLFDDLPAARVLAIASPAVREH
                     LPRLQDLGGPGGRPTHVLLCDPSYGATYDDLDAAFRAVRDGAELVATQLGRVARRDDG
                     EHLDTGGWVRLLEYATGVRARVLGKPSPEFFRLALELVGGTPDRALVVGDDRAADVGG
                     ARTIGARSVLVRTGKGARPPGGGDGDGPEPDAEVDSLADVPGLLRRVG"
     gene            1749..2960
                     /locus_tag="VEP73_01430"
     CDS             1749..2960
                     /locus_tag="VEP73_01430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018745996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT25135.1"
                     /translation="MPPVPLPSDAPEDGRRPGRGGVVFLNWRDTRNPEGGGSELFVER
                     VAAGLAAQGRPVTIVCAAWRGAPREELADGVRILRRGSRLSVYLRAFLLYASGRVGPH
                     GVVVDVQNGLPFFSTLYARAPVVVLVHHVHREQWRVVLPPLPARVGWWIESRLAPRLY
                     RGARYVAVSESTRQELVRLGVRPDAIALVHNGTEIPPATAPTRTPCPSICVLGRLVPH
                     KRVELALEAAASLRPHVPGLKVTVVGQGWWEPRLREAVARLGLEDTVELLGFVDDATK
                     HAVLGRSWVLAMPSLKEGWGLAVLEAAGQATPTVAFRSAGGLAESVIDGRTGLLADDP
                     AQFTAHLERILADRPLRERLGAAARDHAAAFSWPQATEAFAGVLDSATATTWAGPSAG
                     AVVDAPGEAQA"
     gene            complement(2968..3108)
                     /locus_tag="VEP73_01435"
     CDS             complement(2968..3108)
                     /locus_tag="VEP73_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25136.1"
                     /translation="MSGITLRVFLGLGVLALALAFLAAVLLVTSQGPSPVSQDLIVYG
                     SR"
     gene            complement(3105..>3333)
                     /locus_tag="VEP73_01440"
     CDS             complement(3105..>3333)
                     /locus_tag="VEP73_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25137.1"
                     /translation="AAAALGLPGSAAAAGLPVAAGAADAAAAALVVWTIGGRPMAPLR
                     RRLLDFQSSRPGLARFRQRGLARLRRGGSDR"
BASE COUNT          413 a         1234 c         1286 g          400 t
ORIGIN      
        1 cgcgaggtag ccgggcctgt cgaagcgccg cgggccggcg acctcgtccg cgaacttgta
       61 gccggggccg ccggtgccgg tcccgagcgg gtcgccgccc tgcaccatga actgcgggat
      121 cacccggtgg aagatggtgc cgtcgtagag cggccggcgc tgctcggccc cggtccgagg
      181 gtccttccag gtcctggtcc cggtcgccag cccgacgaag ttctcgaccg tcctgggggc
      241 ggtgtccggg aacagggcga cccggatgtc gcccttagag gtcttcagcg tcgcggtctt
      301 cagcgtcgcg tgggtgtcgg ccatcgtgct cgctcctgtc gtgaggttcg tacgcctacg
      361 gcgggaaggt gccgtcccgt cacggccacc ctacccgacc cggcggagga ggccgggcac
      421 gtcggccaga ctgtccacct cggcgtccgg ctccgggccg tccccgtcgc ccccgccggg
      481 cgggcgcgcg cccttgccgg tgcggaccag gaccgaccgg gcgccgatgg tgcgggcgcc
      541 gccgacgtcg gcggcacggt cgtcgcctac cacgagcgcg cggtcgggcg tgccgccgac
      601 cagctcgagt gccaggcgga agaactccgg cgacggcttg ccgaggacgc gggcacgcac
      661 gccggtggcg tactcgagca ggcgcaccca cccgccggtg tccaggtgct cgccgtcgtc
      721 gcgcctggcg acgcgcccga gctgggtggc caccagctcg gcgccgtcgc ggacggcccg
      781 gaaggcggcg tccaggtcgt cgtaggtcgc cccgtagctg ggatcgcaca gcagcacgtg
      841 ggtgggccgg ccgcccggcc cgcccaggtc ctgcagccgc ggcaggtgct cgcggaccgc
      901 gggcgaggcg atggcgagca ccctggccgc cggcaggtcg tcgaacagcc gcctggccag
      961 gacgaccggg gtcaggaagt cgtcctcggc ggccgggatg cccatgccgg ccaggcgggc
     1021 ggccagggcg gcgggcgagg tcgagtcggt gttgctcgtg aaccggacgg cgaggccgtc
     1081 gcggcggagc cgctcgaccg cctcgccggc acccggcacc ggccgggtgc ccacgtacac
     1141 ggtgccgtcg aggtcgagca acgcgacctc gacgtcgtcg aagaccacca tggtcgtcca
     1201 cctctccccc agcgcgtccc ggcggcacaa ctctcggcgc cacgcccgcg cggggtcaac
     1261 cagggacgtc agccagggac gtcagccagg gacggggagg ggtggggcct tggccaccga
     1321 ccggtcccgg ggacggcacc ggtgcccgcg agcgggcgga tggtacggtg tccgagtccc
     1381 tcgagggcac acgcgtgcga cgagccgcgt gacccccctt ggccctcgaa ggctgctccg
     1441 gaaccggtcg gtagcgagcg ggttatatgg tacctgcaag agcctcgcaa ggaccattcg
     1501 cgggaacggc ccggaatggc taggcgacct gcggcgggac gacgcgaccc gggggcgccc
     1561 gcgtggcggg cacggcccag aatggtcggg gtcgccgggt cgcgagcggg ggggccgccc
     1621 gcgcgcccag gacgagccgg cccgacgatc ccggcagagc ccgacgaagc tcccgttaca
     1681 aacagaatcg ggatgattgc agacgggtgg gggcaaggtg ggataggttc gccccgccga
     1741 tgtccgagat gccgcccgtg cccctgccgt cggacgcccc cgaggacggc aggcgcccgg
     1801 gccgcggcgg cgtggtcttc ctcaactggc gcgacacacg caacccggaa ggtggcggct
     1861 cggagctgtt cgtcgagcgg gtggccgccg gcctcgccgc tcaaggccgg ccggtgacga
     1921 tcgtgtgcgc cgcgtggcgg ggcgccccgc gcgaggagct ggccgacggc gtccgcatcc
     1981 tgcgccgggg ctcgcggctc agcgtgtacc tgcgcgcgtt cctgctctac gcgagcggcc
     2041 gcgtcgggcc gcacggcgtg gtggtggacg tccagaacgg cctgccgttc ttctcgaccc
     2101 tgtacgcgcg ggcgcctgtg gtcgtgctcg tccaccacgt ccacagggag cagtggcggg
     2161 tggtgctgcc cccgctgccg gcccgggtcg gctggtggat cgagtcccgg ctcgccccgc
     2221 gcctctaccg gggggcgcgc tacgtggcgg tgtcggagtc gaccaggcag gagctcgtgc
     2281 gactgggcgt ccggcccgac gcgatcgccc tcgtccacaa cgggaccgag atcccgcccg
     2341 cgaccgcccc gacccggacg ccctgcccgt ccatctgcgt gctcggccgg ctcgtgcccc
     2401 acaagcgtgt cgagctggcg ctcgaggccg ccgccagcct ccgcccgcac gtccccgggc
     2461 tcaaggtgac ggtggtcggc cagggctggt gggagccgcg gctccgcgag gcggtggccc
     2521 ggctcggcct cgaggacacg gtggagcttc tcgggttcgt cgacgacgcg accaagcacg
     2581 cggtgctggg ccgctcgtgg gtgctcgcca tgccctcgct caaggagggg tgggggctcg
     2641 ccgtgctgga ggcggccggg caggccaccc cgacggtcgc gttccgctcg gccggcggcc
     2701 tcgccgagtc cgtgatcgac gggcgcaccg gcctgctggc cgacgacccg gcccagttca
     2761 cggcccacct cgagcggatc ctggccgacc ggccgctgcg ggagcgcctg ggcgccgccg
     2821 cgcgcgacca cgccgcggcc ttcagctggc cgcaggcgac ggaggccttc gccggcgtcc
     2881 tcgacagcgc gacggcgacg acctgggcgg gtccgtcggc gggcgccgtg gtcgacgccc
     2941 ccggggaggc acaggcgtga ccggcggcta ccgcgagccg tagacgatga ggtcctggga
     3001 gacggggctc gggccctggc tcgtcaccag caggacggcg gccaggaacg cgagcgccaa
     3061 cgccagaacg ccgagaccca ggaacacgcg cagcgtgatg ccactcaccg gtcacttcct
     3121 cctcgcctca agcgcgccag gccacgctgc ctgaagcgcg ccaggcccgg tcgggaactc
     3181 tggaaatcta gcagccgccg ccggagcggc gccataggtc taccgccgat cgtccagacg
     3241 accagcgccg ccgcggcggc gtcggccgcg ccggcggcga ccggcagccc ggcggccgcg
     3301 gccgagcccg gcaggccgag cgccgcggcg gcc
//
LOCUS       DASPYY010000100         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1842984, whole genome shotgun sequence.
ACCESSION   DASPYY010000100 DASPYY010000000
VERSION     DASPYY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842984"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1659
                     /locus_tag="VEP73_01445"
     CDS             <1..>1659
                     /locus_tag="VEP73_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25138.1"
                     /translation="IGLAAAEEQRLSGELDRAGARLEDFRRARRELPDTGPVARAADA
                     VGKRAVLLARARDETTAARTALDAAIAEVDAMTRRLRQAAAERRMPAEAGQVDAVARA
                     AADFENAATQLHAERAKLAQAREDLAAQTEAIERRRTEYAEAADALAERERQQQALEE
                     EFRALEETLQADVQQVLEQIRETERLLAAASQAYRDLDARARAEHDRATEAQAGLRGE
                     RQSLADAVGQLYEQAAQFAEYARPDLRPLAGVSGVSGVSGVSGVTAAALWPWPDRARW
                     PDPAQAAADLATALAGGAPGDVDPGEAVRGVLPPDAVGILDAFAAANPKTRGGRQVTE
                     GTLKNTADRMSVALKDFTEALAACDEDYRVDWEPGAVVTVHVIADEGRKPVAEFATRI
                     AERAADQGVLLEDRERKVLEDELLAALAQQIHDRVLAARDLTRNMNADTRSKPMSSGI
                     AVGIRWAQSDKITDRQRATSRLLERDSPGPERLAELRGLLREMIREYRGGHPRATYRE
                     ALAACDEDYRVDWDPGAVVTVHVIDDEGRKPVAQFATRIAERAV"
BASE COUNT          237 a          615 c          642 g          165 t
ORIGIN      
        1 tgatcgggct ggccgccgcc gaggagcagc ggctatccgg tgagctggac cgggccggcg
       61 cgcggctcga ggacttccgc cgcgcccggc gcgaactgcc ggacacgggc ccggtcgcgc
      121 gggccgccga cgcggtcggc aagcgcgccg tgctgctcgc ccgcgcgcgg gacgagacca
      181 cggcggcacg gacggccctg gacgctgcga tcgccgaggt cgacgctatg accaggcggc
      241 tccggcaggc cgccgccgaa cgccggatgc cggccgaggc cgggcaggtg gacgcggtgg
      301 cgcgggcggc cgcggacttc gagaacgccg cgacccagct gcacgccgag cgggccaagc
      361 tggcccaggc gcgcgaggac ctcgccgcgc agaccgaggc gatcgagcgg cgccggaccg
      421 agtacgcgga ggccgccgac gcgctggccg aacgggaacg gcagcagcag gcgctggaag
      481 aggagttccg cgcgctcgag gagaccctgc aggcggacgt ccagcaggtc ctggagcaga
      541 tcagggagac ggaaaggctc ctcgcggcgg ccagccaggc ctaccgcgac ctcgacgccc
      601 gcgcccgggc cgagcacgac agggcgaccg aggcccaggc cgggctgcgc ggcgagcggc
      661 agtcgctggc cgacgcggtc gggcagctct acgagcaggc cgcgcagttc gccgagtacg
      721 cccggcccga cctgcgcccg ctggccgggg tctccggggt ctccggggtc tctggggtct
      781 ctggggtcac ggccgccgcg ctctggccct ggcccgatcg agcccgctgg cccgaccccg
      841 cccaggccgc cgcggacctg gccacggcgc tggccggcgg tgcgcctggc gacgtggacc
      901 cgggggaggc cgttcgcggc gtgctcccgc cggatgcagt cgggattctc gacgcgttcg
      961 ccgccgccaa cccgaagacc cgcggcgggc gccaggtcac cgagggcacg ctgaagaaca
     1021 ccgcggaccg gatgtccgtc gcgctcaagg acttcaccga ggccctggcc gcctgcgacg
     1081 aggactaccg ggtggactgg gaaccgggtg ccgtcgtcac cgtgcacgtg atcgccgacg
     1141 agggccgcaa gcccgtcgcc gagttcgcca cgaggatcgc cgagcgcgcc gccgaccagg
     1201 gcgtgctcct ggaagaccgg gaacgcaagg tgctcgagga cgagctgctg gccgcgctcg
     1261 cccagcagat ccacgaccgg gtgctcgccg cgcgggacct caccaggaac atgaacgccg
     1321 acacgaggtc caagccgatg tcgtccggga tcgcggtcgg catccgctgg gcgcagtccg
     1381 acaagatcac cgaccggcag cgggccacgt cccggttgct cgagcgcgac tcgccgggcc
     1441 cggagcggct cgccgagctg cgcggcctgc tccgcgagat gatccgggag taccggggcg
     1501 gccacccgcg ggccacctac cgggaggcgc tggccgcctg cgacgaggac taccgggtcg
     1561 actgggaccc cggcgccgtg gtcaccgtgc acgtgatcga cgacgagggc cgcaagccgg
     1621 tcgcccagtt cgccacccgg atcgccgaac gtgccgtcg
//
LOCUS       DASPYY010000101         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1562297, whole genome shotgun sequence.
ACCESSION   DASPYY010000101 DASPYY010000000
VERSION     DASPYY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562297"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..787
                     /locus_tag="VEP73_01450"
     CDS             161..787
                     /locus_tag="VEP73_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HYT25139.1"
                     /translation="MPPRYPILVDLAGRQAVVAGGGPVAERKARGLLAAAAAVRVVSP
                     EVTPGLAALAERGEVTVRARPWRPGDLDGAVLVVAATGDPASNREVAEQGRSRGVLVN
                     LVDDPDHGGFTVPAVLRRGDLTVAVATSGRTPGLAGALRRRLEGQFGPEWAELVDLLA
                     ADRGSLPGAADQAAWDALLAPEVLEAVRRGQPAVVRERIRACRSSSSA"
     gene            763..2037
                     /locus_tag="VEP73_01455"
     CDS             763..2037
                     /locus_tag="VEP73_01455"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYT25140.1"
                     /translation="MPVFVVGLNFKSAPLELLERLAVDPERRPKALAHLLSQDHVHEA
                     VVLSTCNRVEVYTAVSRFHGGAADVRRFLAEFHHLDAQDFADRLYDYYEERAVQHLFA
                     VASGIDSMVVGEAQVLGQVREAFQAATAEHAVGPVLSTLFRQAIKVGRRARAETGIGA
                     ALASTLTVGMRVAEGQLGSLAGRRVLVVGAGRMGRLAARAARAAGAGELVVANRSAGN
                     GATLARELGGRSVPLDRVEDELAAADLAIASTAAVAPTVTAPVMAAAVARRDRAPLVV
                     LDLGVPRDVDPEVRALPGVMLADLDGLRAVLETGDSGRRDEVDRVRAMIAGETAGFMS
                     WQREARLGPTIRSLRARAEEARRRELARAGSRLAGLDERERAAVEAVTRGLVNKLLHD
                     PVVRGKALAAGPDGDLYARMLRELYALDDPDA"
BASE COUNT          227 a          837 c          818 g          248 t
ORIGIN      
        1 gacctctcga ccgagctgca gatcctgtcc ttccacgagc agcgccgggc cgccgtcgac
       61 ctggtccgcc aggccgccgc caggcccgtg ggcgccgggc cgggcgctcg cgagggcgcc
      121 gggccggacg ctcccggggc cagaggctga cccgccggtc atgccgcccc ggtaccccat
      181 cctcgtcgac ctcgccggcc gccaggcggt ggtggccggc ggcggcccgg tggccgagcg
      241 gaaggcacgc gggctgctcg ccgccgccgc ggcggtgcgg gtggtgagcc cggaggtgac
      301 gcccggcctg gcagcgctgg ccgagcgggg cgaggtcacc gtccgggcac ggccctggcg
      361 gccgggcgac ctcgacggcg ccgtcctcgt ggtcgccgcc accggcgacc cggccagcaa
      421 ccgcgaggtg gccgagcagg gccgctcccg aggggtgctc gtgaacctcg tcgacgaccc
      481 cgaccacggc ggcttcaccg tgcccgcggt gctccgccgc ggcgacctca cggtcgcggt
      541 cgccacctcc gggcggacgc ccggcctggc gggggcgctc cggcggcgcc tcgaggggca
      601 gttcgggccg gaatgggccg agctggtcga cctgctggcc gccgatcggg gtagccttcc
      661 gggggccgcc gaccaggcgg cctgggacgc cctcctcgcc cccgaggtgc tcgaggccgt
      721 ccgccgtggc cagccggccg ttgtacgcga gaggatccgg gcatgccggt cttcgtcgtc
      781 ggcctgaact tcaagagcgc cccgctcgag ctgctcgagc ggctcgcggt cgaccccgag
      841 cgtcgcccca aggcgctcgc ccacctgctc tcccaggacc acgtccacga ggcggtggtg
      901 ctgtcgacct gcaaccgggt cgaggtctac accgcggtca gccgcttcca cggcggcgcg
      961 gcggacgtgc gccgcttcct ggccgagttc caccacctgg acgcgcagga cttcgccgac
     1021 cgcctctacg actactacga ggagcgcgcg gtccagcacc tgttcgccgt ggcctccggc
     1081 atcgactcga tggtggtcgg cgaggcccag gtcctcggcc aggtgaggga ggcgttccag
     1141 gccgcgaccg ccgagcacgc cgtcgggccc gtcctgtcga ccctgttccg ccaggcgatc
     1201 aaggtgggcc gccgggcccg ggccgagaca gggatcggcg cggcgctcgc ctccaccctc
     1261 accgtcggca tgcgggtcgc cgagggccag ctcggcagcc tcgccggccg ccgggtgctg
     1321 gtcgtcgggg cgggccggat gggccgtctg gccgccaggg cggcgcgggc ggccggcgcg
     1381 ggcgagctcg tggtcgcgaa ccgctccgcc ggcaacggcg ccaccctcgc ccgcgagctc
     1441 ggcgggcgct cggtccccct cgaccgggtc gaggacgagc tcgccgccgc cgacctcgcg
     1501 atcgcctcga ccgcggccgt cgccccgacc gtcaccgccc cggtgatggc cgcggccgtc
     1561 gcccgcaggg accgcgcccc gctcgtcgtg ctcgacctcg gggtgccgag ggacgtcgac
     1621 cccgaggtgc gcgccctccc cggggtgatg ctcgccgacc tggacgggct cagggccgtg
     1681 ctcgagaccg gggacagcgg ccggcgcgac gaggtcgacc gggtccgcgc catgatcgcc
     1741 ggggagaccg ccgggttcat gagctggcag cgggaggcgc ggctgggccc gacgatccgt
     1801 tccctgcgcg cccgcgccga ggaggcccgc aggcgggagc tggccagggc cggttccagg
     1861 ctcgccgggc tggacgagcg cgagcgggcg gcggtcgagg cggtcaccag ggggctcgtc
     1921 aacaagctcc tgcacgaccc ggtcgtgcgg ggcaaggccc tggcggccgg ccctgacggc
     1981 gacctgtacg cccgcatgct gcgcgagctc tacgccctgg acgaccccga tgcctgaagc
     2041 gcgcgaggcc ccctcgcctg aagcgcgcca ggccccggtg cctgaggggc cgccgctccg
     2101 tctcgccacg cgcggcagcg ccctcgcgct
//
LOCUS       DASPYY010000102         6561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2404849, whole genome shotgun sequence.
ACCESSION   DASPYY010000102 DASPYY010000000
VERSION     DASPYY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404849"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VEP73_01460"
     CDS             <1..1080
                     /locus_tag="VEP73_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT25141.1"
                     /translation="PYAETTEAFLAGLQQADDQGVALQYLSALPVEEKSVFDYNQGNP
                     TGNPETLGKHPRPRVQLVAVYPKEGTLESDHPWLVMHAPWVTDDKRQAAAGFLAYLRS
                     APVQARFTAAGFRTWQGTPGPVATLANGLSADQPRVVMPFPPPQVVQLIAASWEKVRK
                     RGNVLAVIDVSGSMNAKVPGTTATKLDLARRAGLSALSLFSDEDNVGLWVFNAGKDGA
                     PDWRVLVPLGRMGDAAGGLPRRQALAGSMQRIVAGGDTGLYDTTLAAVRYVQRNWVPG
                     RINTVVLLTDGKNEKQGGHTLPALLSALRETRGRPVRVITIAYGADADRSVLRQIAAV
                     TQGAAYVAADPKEIERIFIAAISHF"
     gene            1338..2651
                     /locus_tag="VEP73_01465"
     CDS             1338..2651
                     /locus_tag="VEP73_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25142.1"
                     /translation="MTGAASGRPGRGAGRRERGLGTEAAEPLGCALGVAAALTAWLLA
                     VPLPAAVLIGAVTWAVRAAAARRRPGLDPGGEGRADPPVAGGNPRVLAGAPARTRGFP
                     PWPALGEEPSPVTATGRSALDRCLAVALAAGGTARRRVERVVDRTATRLTHARHDRDR
                     HARHDRDRGGSPVAGDPPPPPGIAAEMLEPATLLLGLAAVAAAVLAGVPTAVAACTGA
                     ATVCVRAAAGALGRRAGPPGQRPASPIDPGSIEAQWLDRARGAAATFSEVVLSATSGP
                     LAEQAPTMRLRIAQTVSVLEGLAARSSAARRVLERTDVQALAAEADRLRAEHAWTSGT
                     VAALLGRSLAAVEARGDAHRQLQAARAGILARVEMGIVHLERLAAGATRLALASAPGP
                     PPSAELDELLESLAALRPGLLEADAPAVDDPAWRAWASGAAASAG"
     gene            2740..4581
                     /locus_tag="VEP73_01470"
     CDS             2740..4581
                     /locus_tag="VEP73_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding and VWA domain-containing
                     protein"
                     /protein_id="HYT25143.1"
                     /translation="MVQQQRRSPRPFLIAVVVAVALVVGLRFLLSGGLSGSGSGGGNG
                     GGGGDCGASPVTVNLTASSEKAQLMRQMAADYQGGRHEVGGRCVQVKVTTKASGAAMA
                     ALARGWNEAVDGPRPDVWSPASSGWVTLLQQRTARADRPKLIPPSVPKVAASPLVIAM
                     PRPMAEALGWPRQKLGWRKLLELAGDRQGWGRFGHPEWGAFRLGKTNPNFSTSGLNAT
                     IGAYFAATNLSNDLSEQDLARPATRDFVRRVEQSVVHYGDTTLTFLSNLQRADDEGAG
                     LTYISAVAVEEKSVWDYNQGNPTGDPATLGRHPKPRTPLAAIYPTEGTLLSDHPYVVL
                     TAPWVDDAKRRAAADFLAYLQGGKAQAKFQQFAFRDYKNNPGPLVTEANGLLRREPAT
                     LLSPPAPAVLDKLLQSWSELRKRANVLLVIDTSGSMAEQVPGTGTSKLELAKRAAVNS
                     LSEFANDDRVGLWMFSRQLDGDTDYRELVPIGRMDEQLGGGRRRAVLTERIRGLPPGG
                     GTGLYDTSLAAYQFVREHQGPGNINAVVLLTDGKNEDDGISLDNLVERLRTERGDQSV
                     RLFTIAYGADADLAVLKRIGETSGGAGYDSSDPTSIDQVFTAVVSNF"
     gene            4627..5385
                     /locus_tag="VEP73_01475"
     CDS             4627..5385
                     /locus_tag="VEP73_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25144.1"
                     /translation="MGLKQELAEPWGLLLGATAGGVAWAAHLPVLVAGGVGVAVWATK
                     AAAAALERRRGLPPAARKLPVDPRTPEGQWLARARTAAGMFADLARGMAAGPLADRVA
                     TMQTEVDDTVQALERLAGQAGAAGQALARIDGRFLAAEGERLRATRAHASGEIAAELD
                     RSLASVQAQQQVHGRLAKARHGVLARLESGTLGLESLVARVVELSAMAAAGPTGDTGA
                     VDQLTDELEGIRQGLAETEEVSRQALNAYHRSEK"
     gene            5414..6355
                     /locus_tag="VEP73_01480"
     CDS             5414..6355
                     /locus_tag="VEP73_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HYT25145.1"
                     /translation="MGKLLKRVWRYVVAALGGKLDEVSDPKIQIEQAIQESKLQHQLL
                     TQQAAAVIGNERELQLKLTRSVEETEKLQANARQALLLADQVRKAGDEQKAATYEDSA
                     RAFASKLVSAEAAMRDMHALHERAGQASAQARAAVENNAMALQQKLAERTRLLSQLDQ
                     AKMQERMNKAMAGMSELAPAGDTPTLGEVRNKIEARYARALGEAELTSNSVEARMLEV
                     ERASIDVEGAARLESIRESLGLATTPAVGADPAPAALDAEASLPAIDAPVEPAAAKAA
                     ARKRAAGTAGTRKASASRRSTAEPPPAEEPHGDAPGS"
     gene            6469..6561
                     /locus_tag="VEP73_01485"
     tRNA            6469..6561
                     /locus_tag="VEP73_01485"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6503..6505,aa:Sec,seq:tca)
BASE COUNT          911 a         2465 c         2419 g          766 t
ORIGIN      
        1 ccctacgcgg agacgaccga ggcgttcctc gccggcctgc agcaggcgga cgaccagggc
       61 gtggccctgc agtacctgtc ggccctgccg gtcgaggaga agtcggtctt cgactacaac
      121 caggggaacc cgaccggcaa cccggagacg ctcggcaagc acccgaggcc gagggtccag
      181 ctcgtcgccg tctatcccaa ggaggggacg ctcgagtcgg accatccctg gctcgtcatg
      241 cacgccccct gggtcaccga cgacaagcgc caggcggcgg ccggcttcct cgcctacctg
      301 cgcagcgccc cggtccaggc ccggttcacg gccgccgggt tccgcacctg gcagggcact
      361 cccgggcccg tcgcgacgct ggccaacggc ctgtccgccg accagccccg cgtggtcatg
      421 cccttccccc ctccacaggt cgtgcagctc atcgcggcga gctgggagaa ggtccgcaag
      481 cgcggcaacg tgctcgcggt gatcgacgtg tcgggatcga tgaacgcgaa ggtcccgggg
      541 accaccgcga ccaagctgga cctggccagg cgggccgggc tcagcgcgct ctcgctcttc
      601 tccgacgagg acaacgtcgg cctctgggta ttcaacgccg ggaaggacgg cgcccccgac
      661 tggcgggtgc tcgtgcccct cgggcgcatg ggcgacgccg cgggagggct cccgaggcgc
      721 caggcgctgg ccggcagcat gcagcggatc gtcgcgggtg gcgacaccgg gctctacgac
      781 accacgctcg ccgccgtgcg ctacgtgcag cgcaactggg tcccgggccg gatcaacacg
      841 gtcgtgctcc tgaccgacgg caagaacgag aagcagggcg gccacaccct gcccgccctg
      901 ctgtccgcgt tgcgggagac caggggacgg ccggtgcggg tgatcacgat cgcctacggc
      961 gccgacgccg accggtccgt cctgcgccag atcgccgcgg tgacccaggg cgccgcctac
     1021 gtcgccgcgg acccgaagga gatcgagcgc atcttcatcg ccgcgatctc gcacttctga
     1081 ccgggccagc cgccggcgcc acccttcgtc cggcggaccg ggagtgcagc ggaccgggag
     1141 tgcactagaa tcctcctgct catcgcgaac ggccgcaccg cgttcgcatc cggttccgcg
     1201 tcgtccacaa ggacgggtga gttggcagga cgccatgtct cgaagcgatc gaggcgcgac
     1261 gggcgctcgt acgccatcgc ggcggccacg gcggtcgtcc tgatcatcct cgtgcgcctg
     1321 ctcatggcgg tcgccgggtg acgggcgccg cctccgggcg ccctggacgg ggcgccgggc
     1381 gccgcgagcg ggggctcggc acggaggcgg cggagcccct cggctgcgcc ctcggtgtgg
     1441 cggccgcctt gaccgcctgg ctgctcgccg tcccgctccc cgccgccgtg ctcatcgggg
     1501 ccgtcacctg ggcggtcagg gcggccgcgg ccaggcggcg cccgggtctc gacccggggg
     1561 gcgaggggcg ggccgaccct cccgtggcgg gcgggaaccc ccgggtcctg gccggggccc
     1621 cagccaggac ccgggggttc ccgccgtggc cggccctcgg ggaggagccg agccctgtca
     1681 cggcgaccgg caggtccgcg ctggaccggt gcctcgctgt ggccctggcg gccggcggga
     1741 ccgcccgccg acgggtggag cgcgtcgtcg accggacggc cacccggctc acgcatgcac
     1801 gccacgaccg ggaccggcat gcacgccacg accgggaccg gggcgggtcg ccggtcgccg
     1861 gcgacccgcc cccgccgccc gggatcgccg ccgagatgct cgagccggcc acgctgctac
     1921 tcggccttgc cgccgtggcg gcagccgtcc tggccggcgt gccgacggcg gtggccgcct
     1981 gcaccggtgc cgcgaccgtg tgcgtccggg ccgcggcggg cgcgctgggc cggcgcgccg
     2041 ggccgcccgg gcagcgcccg gcctccccca tcgacccggg atcgatcgag gcgcagtggc
     2101 tggaccgggc ccgcggcgcg gccgcgacct tctccgaagt cgtcctcagc gcgacctccg
     2161 ggccgctcgc ggagcaggcg ccgacgatgc gcctgcggat cgcccagacg gtcagcgtcc
     2221 tcgaggggct cgccgcgcgg tcgagcgctg ccaggcgcgt gctcgagcgc accgacgtgc
     2281 aggccctggc cgccgaggcc gaccgcctcc gcgcagagca cgcctggacg tccggcacgg
     2341 tggcggccct cctcggccgc tcgctggccg cggtggaggc ccgcggggac gcccatcgcc
     2401 agctgcaggc ggcacgagcc ggcatcctgg cccgggtcga gatgggcatc gtccatctgg
     2461 agcgcctcgc ggcgggggcg accaggctgg ccctcgcgag cgcccccggg ccgcccccct
     2521 ccgccgagct cgacgagctg ctcgagagcc tggccgccct ccgccccggg ctgctggagg
     2581 ccgacgcgcc ggccgtcgac gaccccgcct ggcgggcgtg ggcctccggc gcggccgcgt
     2641 ccgccgggta gccgggcgcg cacctgccca cgcgggcgcc ctccatgtcg ttgaatgaga
     2701 gggagcaggc tcgactcagc ccgggtccga ggaggatcgg tggtccagca gcagcggcgc
     2761 agcccgcgcc ccttcctgat cgcagtcgtg gtggccgtcg cgctcgtcgt cggcctgcgg
     2821 ttcctcctct ccggaggcct ctccggctct ggctccggtg gcggcaacgg aggtggcggc
     2881 ggggactgcg gcgccagccc ggtgacggtc aacctcacgg cgtcgtcgga aaaggcccag
     2941 ctcatgcggc agatggcagc cgactaccag ggcgggcggc acgaggtcgg cgggcgctgc
     3001 gtccaggtga aggtgaccac caaggcgtcc ggggcggcga tggcggcgct ggcgcggggc
     3061 tggaacgagg cggtggacgg gccccggcca gacgtctggt cgcccgcctc ctcgggatgg
     3121 gtcaccctgc tccagcaacg cacggccagg gccgacaggc cgaagctgat ccccccgagc
     3181 gtgcccaagg tcgccgcctc gcccctggtg atcgcgatgc cgcgcccgat ggccgaggcg
     3241 ctcggctggc cgaggcagaa gctcggatgg cgcaagctcc tcgagctcgc gggcgaccgg
     3301 cagggctggg gcaggttcgg ccaccccgaa tggggcgcgt tccggctcgg caagaccaac
     3361 cccaacttct cgacgtcagg gctgaacgca accatcgggg cctacttcgc cgccacgaac
     3421 ctctccaacg acctgagcga gcaggacctc gcccgccccg cgacccgcga cttcgtccgc
     3481 cgggtcgagc agtcggtcgt gcactacggc gacacgaccc tcacgttcct gtccaacctg
     3541 cagcgggccg acgacgaggg cgccgggctc acctacatct cggccgtcgc cgtcgaggag
     3601 aagtcggtct gggactacaa ccagggcaac cccaccggcg acccggccac gctcggcagg
     3661 cacccgaagc cgaggacccc gctcgccgcc atctacccga ccgagggcac cctgctctcc
     3721 gaccacccgt acgtcgtgct caccgccccc tgggtggacg acgccaagcg cagggcggcg
     3781 gccgacttcc tcgcctacct gcagggcggc aaggcgcagg ccaagttcca gcagttcgcg
     3841 ttccgcgact acaagaacaa ccctgggccg ctcgtcaccg aggccaacgg cctgctgcgg
     3901 cgcgaaccgg cgaccctgct gtcgccgccg gcgcccgccg tgctcgacaa gctgctgcag
     3961 agctggagcg agctgcgcaa gcgcgccaac gtcctcctcg tcatcgacac ctcggggtcc
     4021 atggccgagc aggtccctgg cacgggcacg tccaagctcg agctcgccaa gcgggcggcg
     4081 gtcaactccc tgtcggagtt cgccaacgac gaccgggtcg gcctgtggat gttctcccgc
     4141 caactcgacg gggacaccga ctaccgggag ctcgtgccga tcgggcggat ggacgagcag
     4201 ctcggcggcg ggcggcggcg ggcggtgctc accgagcgga tcagagggct gccgcccggg
     4261 ggcggcaccg gcctgtacga caccagcctg gccgcctacc agttcgtgcg cgagcaccag
     4321 ggcccgggca acatcaacgc ggtcgtgctg ctcaccgacg ggaagaacga ggacgacggc
     4381 atctccctcg acaacctggt cgagcgcctg cggaccgagc ggggcgacca gtcggtgcgg
     4441 ctgttcacca tcgcctacgg cgccgacgcc gacctcgctg tcctcaagcg gatcggcgag
     4501 acctcgggcg gcgccggcta cgactcttcc gacccgacaa gcatcgacca ggtgttcact
     4561 gcggtagtct cgaacttctg aaagggggtc cggggcgcat cgggcggacc gccggacgag
     4621 acgacgttgg gactcaagca agagctggcc gagccttggg gcctgctgct cggggcgaca
     4681 gccggcgggg tggcctgggc ggcgcacctt cccgtcctcg tggccggcgg cgtcggggtg
     4741 gccgtgtggg cgacgaaagc ggccgcggcg gcgctggagc ggcggcgcgg cctgccgccg
     4801 gccgcgcgga agctgccggt cgacccgcgc acgccggagg gccagtggct ggcgcgggcg
     4861 aggacggcgg cgggcatgtt cgccgacctg gcccgcggga tggccgccgg gccgctcgcc
     4921 gaccgggtcg ccacgatgca gacggaggtg gacgacaccg tgcaggcgct ggagcgtctg
     4981 gccggccagg cgggcgcggc cggccaggcg ctcgcccgca tcgacggccg cttcctcgcc
     5041 gccgagggcg agcggctcag ggcgacccgc gcgcacgcgt ccggcgagat cgcggccgag
     5101 ctggaccgct cgctggcgtc ggtgcaggcg cagcagcagg ttcacggccg gctggccaag
     5161 gcgagacatg gggttctcgc acggctcgag tcgggcaccc tgggcctcga gagcctggtc
     5221 gcccgcgtcg tcgagctgtc ggcgatggcg gcggcgggcc cgaccggcga cacgggcgcc
     5281 gtcgaccagc tcaccgacga gctggagggc atcaggcagg ggctggccga gacggaagag
     5341 gtgtcgaggc aagcgctcaa cgcctaccac cgttccgaga aatgagagag gcgcgaccga
     5401 ggaggtcagg tagatgggca agctgctcaa gcgggtgtgg cgctacgtcg tggcggcgct
     5461 cgggggcaag ctcgacgagg tgtccgatcc caagatccag atcgagcagg ccatccagga
     5521 gtccaagctg cagcaccagc tcctgaccca gcaggccgcc gcggtcatcg gcaacgagcg
     5581 tgagctgcag ctcaagctca cccgctcggt cgaggagacc gagaagctgc aggcgaacgc
     5641 ccgccaggcc ctcctcctgg ccgaccaggt gcgcaaggcg ggcgacgagc agaaggcggc
     5701 gacctacgag gactcggccc gggccttcgc tagcaagctc gtcagcgccg aggcggccat
     5761 gcgcgacatg catgcgctgc acgagcgggc cggccaggcg tccgcccagg cccgcgcggc
     5821 ggtcgagaac aacgcgatgg ccctgcagca gaagctggcc gagcggaccc ggctcctgtc
     5881 gcagctcgac caggccaaga tgcaggagcg catgaacaag gccatggccg gcatgagcga
     5941 gctggccccg gccggcgaca cgccgaccct cggcgaggtg cgcaacaaga tcgaggcccg
     6001 ctacgcgcgg gcgctcggcg aggcggagct gacctcgaac tcggtcgagg cgcgcatgct
     6061 cgaggtggaa cgagcctcca tcgacgtcga gggcgccgcc cgcctcgagt cgatccgcga
     6121 gagcctgggg ctcgccacca ccccggcggt cggcgccgac cccgctcccg ccgcgctcga
     6181 cgccgaggcg tccctgcccg ccatcgacgc gccggtcgag cccgcagccg cgaaggccgc
     6241 cgcccgcaag cgcgccgccg gcacggccgg cacgagaaag gcgtcggcct ccaggcgctc
     6301 gaccgccgag ccgcccccgg ccgaggagcc gcacggcgac gcgccagggt catgatgcgg
     6361 ccccccacca cgccgacgtg acgaccgatc acgaccgtcc ccgggccttg ggcggtgcgc
     6421 cgccatcgtt gatctgcacc taccggtctg ccaggtacaa tcccgcgcgg aggcgtccgt
     6481 ggcctggtgg ccgccccggt cttcaaaacc ggtgagggga gcgtccctcc cctggcgggt
     6541 ccgattcccg tccgcctccg c
//
LOCUS       DASPYY010000103         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2123705, whole genome shotgun sequence.
ACCESSION   DASPYY010000103 DASPYY010000000
VERSION     DASPYY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123705"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VEP73_01490"
     CDS             complement(<1..196)
                     /locus_tag="VEP73_01490"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HYT25146.1"
                     /translation="MPIATPDVYAEMLDRAKAREFAYPAVNVTSSETLNAALAGFAEA
                     GSDGIVQITTGGAEFLSGQKV"
     gene            complement(231..509)
                     /locus_tag="VEP73_01495"
     CDS             complement(231..509)
                     /locus_tag="VEP73_01495"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HYT25147.1"
                     /translation="MSLGASIFLIVIGAILAFAVHVSTSGFDVNTVGVILMAAGLVGL
                     VLTLVLSSGSRYRRRRSVVGPDTIVEERPVIRERRVERDVDVPPSDIP"
     gene            660..1274
                     /gene="idi"
                     /locus_tag="VEP73_01500"
     CDS             660..1274
                     /gene="idi"
                     /locus_tag="VEP73_01500"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HYT25148.1"
                     /translation="MTTSQPSERGAGRAVERVVLLGRDGEPVGEADKAAVHGASTPLH
                     LAFSCYAFDRSGRLLVTRRSREKRTFPGTWTNTCCGHPAPGEAVAHAVRRRLRDELGL
                     EAAGLRLALPDFAYRASANGVEENELCPVFLCRVAADPTPDPSEVDGWHWRSWAAFVD
                     DAASPGSELSVWSRLQVARLEEEGQVTRFLRDTPPSPPSSSRPA"
     gene            1360..2031
                     /locus_tag="VEP73_01505"
     CDS             1360..2031
                     /locus_tag="VEP73_01505"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT25149.1"
                     /translation="MLPDRRHGRHADRLHGRTVRIVRPRDSEALLDEDAFEREEFLPY
                     WAQLWPSAVALAGAVWARAPREARTLELGCGLGLPSIAAAMAGGRVLATDWSADAVAF
                     TAANAARNGVALSTAVCSWTEPGPFVDRAPWDLVLAADVLYEQRNVQPLLELLPRLVG
                     GAGEVWVADPGRAAAAAFLAGASAVWEVRSAADALLPGGAVHRLRRRRPTASRQGPRP
                     AASPR"
     gene            complement(1985..>2950)
                     /locus_tag="VEP73_01510"
     CDS             complement(1985..>2950)
                     /locus_tag="VEP73_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25150.1"
                     /translation="LPVTRTVTENELRATGDSVTHAEDELATVDTAAGRGYSRRLLIA
                     GVGVTSLTVDTKLMAYLPKALRPQASDFLVIAFGMGGTYRAGLKAGLRTDVVELSPTV
                     PSRMPVFFPDADRYLHHPDGRVITSDGRNYVRLARKRYDLVAVDPAPPIESAGSVVLY
                     TREFLTEGRARLKPGGVFLLWMPYALPLDDFKAHVRTFDRVFAHTSLLLAPGHHGVYL
                     LGSDAPMTFSDEQVERVLGSPEARSDLADVPDYPPTDARGWVRAVHRAQWLTDGQVRA
                     FTGPGPEITDDRPRSEYFLWRRAFLADKTYINEAKLRAAGPGGSP"
BASE COUNT          360 a         1067 c         1139 g          384 t
ORIGIN      
        1 tgaccttctg gccggacagg aactccgcgc cgccggtggt gatctgcacg atgccgtcgc
       61 tgcccgcctc cgcgaagccg gcgagggcgg cgttgagcgt ctccgaggag gtcacgttga
      121 ccgcggggta ggcgaactcg cgagctttcg cccggtcgag catctcggcg tacacgtcgg
      181 gagtggcgat cggcacggcg gtcctccctc ccgggcgctc gccgaggacg tcaggggatg
      241 tcgctcggcg ggacgtccac gtcccgctcg acgcggcgct cccggatgac cggccgctcc
      301 tcgacgatgg tgtccgggcc gacgacggag cggcggcggc ggtagcggga gccgctcgag
      361 agtaccagcg tcaggaccag cccgaccagg cccgcggcca tcaggatgac gccgaccgtg
      421 ttgacgtcga atcccgaggt gctgacgtgg acggcgaacg cgaggatcgc gccgatgacg
      481 atcaggaaga tgctcgcacc gaggctcatg gggtctccct ctcgtgggtt gctcgtctgc
      541 cagagcagac ctggtgcggg cactacccat ccccgaagag cgggaaaccg ccgaggggcg
      601 cgcgccgggc gctcccggta tcgtcggcgt caccgtgcac cctcccgtgg aggcccgccg
      661 tgacgaccag ccagccgagc gagcgcggag ccggacgagc ggtcgagcgc gtcgtcctgc
      721 tcgggcggga cggcgagccg gtcggggagg ccgacaaggc cgccgtgcac ggcgcgtcga
      781 cccccctgca cctggccttc tcctgctatg ccttcgaccg ctccgggcgg ctgctggtca
      841 cgcggcggtc ccgggagaaa cggacctttc ccggcacctg gacgaacacc tgctgcgggc
      901 accccgcccc gggcgaggcc gtcgcccacg ccgtgcggcg ccgcctgcgc gacgagctcg
      961 gcctggaggc ggccggcctg cggctcgcgc tgccggactt cgcctaccgc gcgagcgcga
     1021 acggcgtcga ggagaacgag ctctgcccgg tcttcctgtg ccgggtcgcc gccgacccga
     1081 cccccgaccc gagcgaggtc gacggctggc actggcgctc ctgggcggcg ttcgtcgacg
     1141 acgccgcgag cccgggcagc gagctgtcgg tgtggtcgcg gctgcaggtg gcccggctcg
     1201 aggaggaggg gcaggtcacg cggttcctgc gggacacccc tccctcgccg ccctcctcga
     1261 gccggcccgc ctgacgtggc cccgccgccg gaggaaggag cacccgggac ggggcgggcc
     1321 acgccctctc catggtgcga cctcgtcgag gaggccgtcg tgctcccaga ccgccgccac
     1381 ggccgccacg ctgaccgcct ccatggccgc acggtccgca tcgtgcgccc gcgcgacagc
     1441 gaggcgctgc tcgacgagga cgccttcgag cgcgaggagt tcctgccgta ctgggcgcag
     1501 ctctggccga gcgccgtcgc gctcgccggg gccgtatggg cccgcgcgcc gcgcgaggcg
     1561 cgcacgctcg agctcggctg cgggctcggc ctgccgagca tcgccgcggc gatggccggc
     1621 gggcgggtgc tggccaccga ctggtccgcc gacgcggtcg cgttcacggc cgcgaacgcg
     1681 gcccgcaacg gcgtcgcgct ctcgaccgcg gtgtgctcct ggaccgagcc gggcccgttc
     1741 gtcgaccggg cgccgtggga cctggtgctc gccgcggacg tgctctacga gcagcgcaac
     1801 gtccagccgc tcctcgagct cctgccgcgc ctggtcggcg gggccggaga ggtctgggtc
     1861 gccgacccgg gccgggcggc cgccgccgcg ttcctcgccg gggcgtcggc cgtctgggag
     1921 gtccgctcgg cggccgacgc cctgctcccc gggggggcgg tccaccggct gcgccggcgg
     1981 cgccctacgg cgagccgcca gggcccgcgg cccgcagctt cgcctcgttg atgtaggtct
     2041 tgtcggcgag gaaggcgcgc cgccacagga agtactccga gcgcggccgg tcgtcggtga
     2101 tctcgggacc ggggccggtg aaggcgcgga cctggccgtc ggtgagccac tgggcccggt
     2161 ggaccgcgcg gacccagccc cgggcgtcgg tgggcgggta gtcgggcacg tccgccaggt
     2221 cgctgcgcgc ctccggggac ccgaggaccc gctcgacctg ctcgtccgag aacgtcatgg
     2281 gggcgtccga gccgagcagg tagacgccgt ggtggccggg ggcgagcagc aggctggtgt
     2341 gggcgaagac acggtcgaag gtgcgcacgt gggccttgaa gtcgtcgagg ggcagggcgt
     2401 agggcatcca gagcaggaac acgccacccg gcttgaggcg cgccctgccc tcggtcagga
     2461 actcccgggt gtagaggacg accgagccgg cgctctcgat cggcggggcc gggtcgaccg
     2521 cgaccaggtc gtagcgcttg cgcgccagcc ggacgtagtt gcggccgtcg ctggtgatca
     2581 cccggccgtc cgggtgatgg aggtagcggt ccgcgtcggg gaagaacacc ggcatcctgc
     2641 tcggcacggt cggcgacagc tcgaccacgt cggtgcggag cccggccttg agcccggcgc
     2701 ggtaggtgcc gcccatcccg aaggcgatca cgaggaagtc gctcgcctgg ggccggagcg
     2761 ccttgggcag gtaggccatc agcttggtgt cgacggtgag cgaggtcacg ccgacccccg
     2821 cgatcagcag gcggcggctg tagcccctcc cggcggccgt gtcgaccgtg gccagctcgt
     2881 cctcggcgtg ggtgaccgag tcgcccgtgg cgcgcagttc gttctcggtc accgtccggg
     2941 tgaccggcag
//
LOCUS       DASPYY010000104         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2264767, whole genome shotgun sequence.
ACCESSION   DASPYY010000104 DASPYY010000000
VERSION     DASPYY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264767"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VEP73_01515"
     CDS             complement(<1..192)
                     /locus_tag="VEP73_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25151.1"
                     /translation="MAVEPEPTPTSARQRRAILATAGLGLLALAATGATILLAGPSRP
                     DAPRLVRAVASAAPTTVPAG"
     gene            complement(197..841)
                     /gene="pilO"
                     /locus_tag="VEP73_01520"
     CDS             complement(197..841)
                     /gene="pilO"
                     /locus_tag="VEP73_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HYT25152.1"
                     /translation="MTLARRELAILVAAAVLVTAAAYVALIRPKVGEVGAAQAAARRA
                     ADQGQTLRAEIKALGDVQADGAALKLRAQAARNLFPDGPDLPDLVDALQKVADQSGVD
                     LLSIQPSAPVASAESPLLASIGTAVSVQGDYFQIEDFLARVEGMVKSPDPGAGIPPRS
                     VVVKSVDISGGGAAGGQGTATAVPASGTTGSDQLTANIALVVFQSAGSPATQRR"
     gene            complement(838..>1505)
                     /locus_tag="VEP73_01525"
     CDS             complement(838..>1505)
                     /locus_tag="VEP73_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25153.1"
                     /translation="GLLVLLVLLALLWAAEAGRADRAGRVAAREEAATAALQAQRAKL
                     QPWAELRDQVDGLERLQAAVYAKEIRFSGVLQDVSTLVPGNAWLTQMSVAFKEQPSTA
                     GTGGSATGGGTATTRSTQQTSGQAAGSAVFPGAPGDGAPVATVTLSGEALGHVDVGGL
                     VRTLNAAARRKGQRVYLNPFFTTSQRQQGSGQAPVTFAATVDLGQAAYSGRFQQPDQP
                     GGK"
BASE COUNT          171 a          561 c          585 g          188 t
ORIGIN      
        1 cccggcaggg acggtcgtgg gggccgcgct cgcgaccgcc cgcacgagcc ggggcgcgtc
       61 gggccgggag gggccggcga gcaggatggt cgcgcccgtg gctgcgagcg cgagcagccc
      121 cagcccggcc gtggcgagga tcgcgcggcg ctgccgggcg ctcgtcgggg tcggttcggg
      181 ttccaccgcc acggacctac ctcctctggg tcgccggcga cccggccgac tggaagacga
      241 ccagggcgat gttcgcggtg agctggtcgg agccggtggt cccgctcgcc ggcaccgcgg
      301 tggcggtgcc ctgcccgccg gcggcgcccc cgcccgagat gtcgaccgac ttcaccacca
      361 ccgagcgggg cgggatcccc gcgccggggt ccgggctctt gaccatgccc tcgacacgcg
      421 cgaggaagtc ctcgatctgg aagtagtcgc cctgcacgct cacggcggtg ccgatggagg
      481 ccaggagcgg gctctccgcg gacgccaccg gcgcggacgg ctggatcgag agcaggtcga
      541 cgccggactg gtcggcgacc ttctgcagcg cgtccaccag gtcgggcagg tcgggaccgt
      601 ccgggaacag gttgcgggcg gcctgggccc gcagcttcag ggcggcgccg tcggcctgga
      661 cgtccccgag cgccttgatc tccgcccgca gggtctggcc ctggtcggcc gcgcgcctgg
      721 cggcggcctg tgcggcgccg acctcgccga ccttgggccg gatcagcgcg acgtaggcgg
      781 cagcggtgac cagcaccgcc gccgcgacga ggatggcgag ctcgcggcgg gccaaggtca
      841 cttccctcct ggctggtcgg gctgctggaa ccggcccgag taggcggcct gcccgaggtc
      901 gaccgtggcc gcgaacgtca ccggcgcctg gcccgacccc tgctgccgct gcgaggtggt
      961 gaagaacggg ttgaggtaga cccgctgccc cttccgcctg gccgcggcgt tcagcgtgcg
     1021 gaccagcccg cccacgtcga cgtggccgag ggcctcgccg gagagcgtga ccgtcgccac
     1081 cggggcgccg tccccggggg cacccgggaa gaccgccgac ccggccgcct ggccgctcgt
     1141 ctgctgggtg ctccgggtgg tggccgtgcc accccccgtt gccgagccgc ccgtgcccgc
     1201 ggtgctcggc tgctccttga acgcgacgct catctgggtg agccatgcgt tgcccgggac
     1261 gagcgtcgag acgtcctgga ggacgccgga gaagcggatc tccttggcgt agacggctgc
     1321 ctggagccgc tcgagaccgt cgacctggtc ccgcagctcg gcccagggct ggagcttcgc
     1381 ccgctgggcc tgcagggcgg cggttgccgc ctcctccctc gcggcgaccc tcccggcgcg
     1441 gtccgcccgg ccagcctccg ccgcccagag cagggcgagg agcacgagga ggacgaggag
     1501 cccgg
//
LOCUS       DASPYY010000105         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1141837, whole genome shotgun sequence.
ACCESSION   DASPYY010000105 DASPYY010000000
VERSION     DASPYY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141837"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VEP73_01530"
     CDS             <1..1236
                     /locus_tag="VEP73_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYT25154.1"
                     /translation="PAIARLAGHRDSWVQRVADSLLAAGLAHATPATAVDLPPTAAAT
                     WPHSAFTWVWPMLVVAEPAADERTAEQHYASLATARRTAGTVSSLTVALANLAVTEVS
                     LGRWPDAIGNATEGLRLATETGQHATAAAFLAILAWLATHQGRADDGRRLAGQALATA
                     VPGGLVAVAADAAWTLAQLDLAGGQPQAALDRLRALATPGHPTAHAPIALLATGELVE
                     AAAHVGALAAVAPRVARFERWAAWDRRAWTQVTAHRCRALLTPGDAAEQHYQAALAVD
                     GLGELPFALARTELLYGEWLRRSRRRAGARPHLRAALELFGRLGATPWAERARSELRA
                     SGETARARDPSTRSQLTPQERQVARLADEGLSNQQIAAQLFLSPHTVSYHLHNVYTKL
                     GITSRVHLRQLDLDGDADR"
     gene            1745..>2365
                     /locus_tag="VEP73_01535"
     CDS             1745..>2365
                     /locus_tag="VEP73_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase"
                     /protein_id="HYT25155.1"
                     /translation="MQDFWDRGLLGLAVDPGFGTAGHNFVYVLYTHDHNPVGNPATWG
                     DTCPTPPGATTDGCPVTGNLSRIPVDPATGVASGPEQPLISDEWCQQFPSHSIGHLAF
                     GPDGNLYVTGGDGASFNNADWGQFGGGAGSPTPANPCGDPPNPVGTPDNPPAAEGGAL
                     RSQSPRRVAGQPILLSGAVLRVDPATGNGVAGNPMYDSQNPSSDASR"
BASE COUNT          301 a          995 c          805 g          264 t
ORIGIN      
        1 ccggccatcg cccgcctggc gggacaccgc gacagctggg tccagcgggt cgccgacagc
       61 ctgctcgcgg ccgggctcgc ccacgccacg ccggccaccg ccgtggacct gccacccacc
      121 gcggccgcca cctggccgca ctccgcgttc acctgggtgt ggccgatgct ggtcgtcgcc
      181 gagccggccg ccgacgagcg caccgccgag cagcactacg ccagcctggc caccgcccgc
      241 cgcaccgccg ggacggtcag cagcctgacc gtggccctgg ccaacctcgc cgtcaccgag
      301 gtctccctcg gccgctggcc cgacgcgatc ggcaacgcca ccgaggggct ccggctggcc
      361 accgagaccg gccagcacgc caccgccgcc gccttcctgg ccatcctggc ctggctcgcc
      421 acgcaccagg gccgcgccga cgacggccgc cggctcgccg gccaggccct ggccaccgcc
      481 gtcccaggtg ggctcgtggc ggtcgccgcg gacgccgcct ggacgctcgc gcagctcgac
      541 ctggccggcg ggcagccaca ggcggcgctg gaccggctgc gcgcgctcgc cacccccggc
      601 cacccgaccg cgcacgcccc gatcgcgctg ctggcaaccg gcgagctggt cgaggcggcc
      661 gcccacgtcg gcgcgctcgc cgccgtggcg ccgcgggtgg ccaggttcga gcggtgggcc
      721 gcgtgggacc ggcgggcctg gacccaggtg accgcccacc gctgccgcgc cctgctcacc
      781 cccggcgacg ccgccgagca gcactaccag gcggccctgg ccgtcgacgg cctcggcgag
      841 ctgcccttcg ccctcgcccg caccgagctg ctctatggcg agtggctgcg gcgctcccgc
      901 cgccgggccg gcgcccgccc ccacctgcgc gccgcgctgg agctgttcgg gcggctcggc
      961 gccaccccct gggccgagcg ggcccgctcg gagctgcggg ccagcggcga gaccgcccgc
     1021 gcccgcgacc ccagtacccg cagccagctc accccgcagg agcgccaggt cgcccgcctc
     1081 gccgacgagg ggctgagcaa ccagcagatc gcggcgcagc tgttcctcag cccgcacacg
     1141 gtcagctacc acctccacaa cgtctacacc aagctcggca tcacctcccg cgtccacctg
     1201 cgccagctcg acctcgacgg cgacgccgac cgctgacggt gccgcgtgca ggatgccgcc
     1261 gacgacgacg tgcacgcggt gaccggccgt cccccgcgca cgctcgcgcg gtccgcgcgc
     1321 gacgccaact gaccaggggc gtccaggccg ggcccagcgg ctgcctcaga cgccggccaa
     1381 accctagcca gagggcccga acgggcgcaa aatggcgcgg ggaggcgtga ccgcccaggg
     1441 cggcgacgta ggacgaatac gacctgcgca ccttctggtg gctggcggcc aggctgacca
     1501 tggtcgaagg agcttcggcg ccggcacggg cggggtgccc tctgccgccg ccgctcgggg
     1561 ggaggcagtc cagtggccac gcacatggcg ggctccaggc tggcgcgcag aggcgcccgc
     1621 cacgccgttt cgctcgcttc gctcgtcgtc ctcgtcacct cgctgctctc cgccggcccg
     1681 gcgtcggcgg ttcgactcgc tctcggaccc cacctccacc caggtcgtcg acctcagcgc
     1741 cgaggtgcag gacttctggg accggggcct gcttggcctg gcggtggatc ccggcttcgg
     1801 caccgccggc cacaacttcg tctacgtgct gtacacgcac gaccacaacc cggtcggtaa
     1861 cccggccacc tggggcgata cctgccccac cccaccgggt gccaccacag acggctgccc
     1921 ggtcaccggc aacctgtccc ggatcccggt cgaccccgcg accggggtgg ccagcgggcc
     1981 ggagcagccg ctgatctccg acgagtggtg ccagcagttc ccctcccact ccatcggaca
     2041 cctggccttc gggcccgacg ggaacctgta cgtgaccggc ggggatggag ccagcttcaa
     2101 caacgcggat tggggccagt tcgggggcgg cgcgggctcg cccaccccgg ccaacccctg
     2161 cggcgaccct cccaacccgg tggggacgcc cgacaacccg cccgctgccg agggcggcgc
     2221 cttgcgctcc cagagcccgc ggcgggtcgc cggccaaccg atcctgctca gtggtgccgt
     2281 gctcagggtc gacccggcca ccggcaacgg ggtggcgggc aacccgatgt acgactcgca
     2341 gaacccgtcc tctgacgcct cacgg
//
LOCUS       DASPYY010000106         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_158476, whole genome shotgun sequence.
ACCESSION   DASPYY010000106 DASPYY010000000
VERSION     DASPYY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158476"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEP73_01540"
     CDS             complement(<1..339)
                     /locus_tag="VEP73_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HYT25156.1"
                     /translation="MTKLQRLYDDHGQSPWLDNLTRPYLRDGTLARLVADGIRGVTAN
                     PTIFAKAIEGSAAYDDQFGALVAAGRSVPDAYWELVVDDVAHALAVLRPTFAAAGGTD
                     GFVSIEVAPEL"
     gene            complement(363..1073)
                     /locus_tag="VEP73_01545"
     CDS             complement(363..1073)
                     /locus_tag="VEP73_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25157.1"
                     /translation="MSDDDFDAQVAGVAALGEPVRRALYRYVVAQPGPVSRDQAAEGA
                     GVPRHVAKFHLDKLAADGLLEIEFRRPAGRGGPGAGRPAKLYRRSSRELRVSLPERRY
                     ELAGHVMAQAIVDAEHHDVPLAKALHGAARGTGRALGEAARRRAGPDAGQAALQAAAL
                     AVLTEHGYEPRTDPGGLTLANCPFHALAQEYTALVCGMNLDLVDGLLAGLERVDLEAR
                     LDPAPGRCCVTVRRRRPA"
     gene            1189..1632
                     /locus_tag="VEP73_01550"
     CDS             1189..1632
                     /locus_tag="VEP73_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25158.1"
                     /translation="MASIPAPRSRATAGPLATGLRENPAYQAFWLLRTGFTVAPILFG
                     LDKFFHVLVNWDRYLAPRIDHLLPGTAHQAMYAVGVVEIVAGLVVALRPRLGGYLVAA
                     WLAGIIVNLLLIPGFYDIALRDFGLLLAALALARLATAFPSSHRA"
     gene            complement(1860..2411)
                     /locus_tag="VEP73_01555"
     CDS             complement(1860..2411)
                     /locus_tag="VEP73_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYT25159.1"
                     /translation="MFERFTRQAREVVVGAQGAARRLGDDRIGTEHLLLGLLAEGTGV
                     AAMVLAGLGVTSEAVEGELAGRRPRPGRLGPADAEALEAIGIDLEAIRRRAEEAFGAG
                     ALERATRRRRGRLPFTKQAKQALEASLREAVALGHHYLGTEHVLLGLLRQPDELAAIV
                     LRALEVPPEVVRARVLAELRRVS"
     gene            complement(2428..2643)
                     /locus_tag="VEP73_01560"
     CDS             complement(2428..2643)
                     /locus_tag="VEP73_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25160.1"
                     /translation="MTETTATELASPDPGVGLRAVAALRQLLEQLEALQVDSARARGW
                     SWQEIAEVLGVSRQAVHKKHAVRLRGR"
     gene            2841..>3467
                     /locus_tag="VEP73_01565"
     CDS             2841..>3467
                     /locus_tag="VEP73_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT25161.1"
                     /translation="MTLAIRAQGLAKRFGGTRALRGIDLEAPTGSVRRGRAAGLLELV
                     DLTEAADRRVKTYSGGMRRRLDLAVSLIGHPRVLFLDEPTTGLDPRSRVGIWAVIREL
                     VAGGTTVLLTTQYLEEADHLADTIVVLDHGRVIARGTPDELKAHAGGQVLEVRPAGPD
                     GLQATARVLAELVGGSPAVDPDTRLASVPVDDPGLLAEVVRRLDRAGVR"
BASE COUNT          444 a         1310 c         1235 g          478 t
ORIGIN      
        1 gagctcgggt gccacctcga tggacacgaa gccgtcggtc ccgccggcgg cggcgaacgt
       61 cgggcgcagc acggccaggg catgggcgac gtcgtcgacg accagctccc agtaggcgtc
      121 gggcaccgag cggccggcgg cgacgagcgc gccgaactgg tcgtcgtagg cggccgagcc
      181 ctcgatggcc ttggcgaaga tcgtcgggtt ggcggtgacg ccacggatgc cgtcggccac
      241 cagccgggcc agcgtcccgt cgcgcaggta ggggcgggtc aggttgtcca gccaggggct
      301 ctgcccatgg tcgtcgtaca gccgctgcag cttggtcatg gtgactcctt ccaggccggc
      361 tttcaggccg gccgccgccg gcgcacggtc acgcagcacc tgccgggtgc ggggtcgagc
      421 cgggcttcga ggtcgacccg ctcgagcccg gcgagcagcc cgtcgacgag gtcgaggttc
      481 atgccgcaga cgagcgcggt gtactcctgg gcgagggcgt ggaacgggca gttcgccagg
      541 gtcaggccgc cggggtcggt tcgcggctcg tagccgtgct cggtgagcac cgcgagggcc
      601 gcggcctgca gggccgcctg gcccgcatcc ggcccggctc ggcgacgggc cgcctcaccc
      661 agcgcgcggc ctgtgcctct ggctgctccg tgcagcgcct tggcgagcgg gacgtcgtgg
      721 tgctccgcgt cgacgatcgc ctgggccatg acgtgcccgg cgagctcgta acggcgctcg
      781 ggcaggctca cccgcagctc ccgtgaggag cggcggtaga gcttggcggg acggcccgcg
      841 ccgggtcccc ctcgcccggc cgggcggcgg aactcgatct cgagcagccc gtcggccgcc
      901 agcttgtcca ggtggaactt tgccacgtgc cgtggcacgc cggcgccctc ggccgcctgg
      961 tcccggctca ccgggcctgg ctgggccacg acgtagcggt acagggcgcg ccgcaccggc
     1021 tcgcccaggg cggccacgcc ggccacctgg gcgtcgaagt cgtcgtcgct cacgggcggt
     1081 gctcctggca cgtacggcct cgccttctaa cggagaattt ggttgacgga acagcgggga
     1141 ggtttctaaa atccatcgta ctgtccgtta gagagggagg caacccagat ggcaagcatc
     1201 cccgcgccgc ggtcccgcgc cacggccggc ccgctggcga ccggcctcag ggagaacccc
     1261 gcctaccagg cattctggct gctgcgtacc gggttcaccg tggccccgat cctgttcggc
     1321 ctcgacaagt tcttccacgt gctggtcaac tgggaccgct acctggcccc ccgcatcgac
     1381 cacctgctcc ccggcaccgc ccaccaggcc atgtacgccg tcggggtcgt cgagatcgtc
     1441 gccgggctgg tggtcgccct ccggcctcgc ctgggcggct acctggtcgc cgcctggctg
     1501 gcagggatca tcgtcaacct gctgctcatc cccggcttct acgacatcgc cctgcgcgat
     1561 ttcgggctgc tgctcgccgc gctcgccctg gcacggctcg ccaccgcctt cccctcctcc
     1621 caccgggcct gaaggccgct gatccaggcg cgtcttatgc ccgggtggat gcggcaggtg
     1681 gcccgctaca acccgctcaa ctgggcggtc gaggccaggg gtgcacctgg tgcagtagcc
     1741 ggcatcacga tccatgacac aggccccgag cgcacgagcc tccccggcaa ggaaagcaag
     1801 ggttacgtgc ggcgacactg ccctgccgtg aggccgccgc acagcaccga gcatcgcggc
     1861 tacgagaccc tgcgcagctc ggcgagcacc cgggcacgca ccacctcggg cgggacctcg
     1921 agcgcccgca gcacgatcgc ggcgagctca tcgggctgcc ggagcaggcc gagcagaacg
     1981 tgctcggtgc cgaggtagtg gtggccgagg gcgaccgcct cccgcaggct tgcctccagc
     2041 gcctgcttgg cctgcttggt gaagggcaga cgacccctgc ggcggcgggt cgcgcgttcc
     2101 agcgcgccgg cgccgaaggc ctcctcggcc ctgcggcgga tcgcctccag gtcgatcccg
     2161 atcgcctcca acgcctcggc gtcggccggg ccgagccgcc ctggccgcgg gcgcctgccc
     2221 gccagctcgc cctcgaccgc ctcgctggtg acgcccagcc cggccaggac catggcggcg
     2281 actccagtgc cctcggcgag caggccgagc agcaggtgct cagtgccgat gcggtcgtcg
     2341 cccagccggc gcgccgcacc ctgtgccccc accacgacct cgcgggcctg cctggtgaac
     2401 cgctcgaaca tccgcgtgct ccttgtgtca gcgcccgcgc aggcggacgg cgtgcttctt
     2461 gtgcactgcc tgccgggaga cgccgaggac ctcggcgatc tcctgccacg accagccgcg
     2521 cgctctggcg ctgtcgacct ggagcgcctc gagctgctcc aggagctggc gcagggcggc
     2581 caccgcccgc agcccgactc ccgggtcggg gctggccagt tcggtcgcgg tcgtctcggt
     2641 catggccgtc atcttaggtt gacaacggcg ccgtgtcaac cgaggttgac agatgccgac
     2701 caggaaggcg gtcacgatcc ggtcccgccc gcacgtcagg gccgtgtcag cggtgtcagc
     2761 ccgccggcag gaccgtggca gccccgcggc gcaccctcct caccacacat gcaatcgacc
     2821 ggcagggagg cagtcgcatc atgaccctcg cgatccgggc ccaagggctc gccaagcgct
     2881 tcggcggcac ccgggcgctg cgcggcatcg acctggaggc acccaccggg agcgtgcggc
     2941 gcggccgcgc cgcggggctc ctcgagcttg tcgacctcac cgaggccgcc gaccgtcgcg
     3001 tgaagaccta ctcgggcggc atgcgccgcc gtctggacct cgccgtgagc ctcatcgggc
     3061 acccccgggt gctgttcctc gacgagccca ccaccgggct cgacccgcgc agccgcgtgg
     3121 gcatctgggc ggtcatccgc gagctggtcg ccggaggcac gaccgtgctg ctcaccaccc
     3181 agtacctgga ggaggccgac cacctcgccg acaccatcgt ggtgctcgac cacgggcggg
     3241 tgatcgcgcg cggcactccg gacgagctga aggcccacgc cggcgggcag gtgctcgagg
     3301 tgcgcccggc cggccccgac ggcctccagg ccacggcgcg ggtgctggcg gagctcgtcg
     3361 gcggcagtcc ggcggtggac cctgacaccc gcctcgcgag cgtgcccgtg gacgacccgg
     3421 gcctgctggc cgaggtggtg cgccgcctgg accgtgccgg cgtccgc
//
LOCUS       DASPYY010000107         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1703600, whole genome shotgun sequence.
ACCESSION   DASPYY010000107 DASPYY010000000
VERSION     DASPYY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703600"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VEP73_01570"
     CDS             <1..867
                     /locus_tag="VEP73_01570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYT25162.1"
                     /translation="MSVFLVVTGFAFKVSAAPFHFWAPDAYEGSPVPVAAYLSVASKA
                     AGFAGLLQVTFVAFPSYARYWAPAFAVLAAFTMLVGNVIALAQTNMVRLLAYSSVAQA
                     GYMLVPFAVGDSGAPDARSKAFQAVLIYLLYYGITNLGAFAVVTLVSQRQPANLLADY
                     DGLGRRSPVLAGAMTLFLLSLAGWPPFAGWYAKFVVFQAALGSGQRSLTVLAVFMAVM
                     TVVAFFYYLGIIGRMWLSPPPEEAAATGALTPSPFAGLAIVLSIAGVIVLGVLPGLLV
                     QYAPMSTLLASR"
     gene            complement(944..2128)
                     /locus_tag="VEP73_01575"
     CDS             complement(944..2128)
                     /locus_tag="VEP73_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25163.1"
                     /translation="MTGFDGVRLVARRELVERTRDRSFLISTLVTLAILCAVIVVPKL
                     LGAGKTPTYDVGLIGTGSRPLAQALTALAPQERVKVRLRQPADLATAEAQLRGGRLDV
                     AVVDGRQLVSKGELDAKLGVLVQAVSRVTRAQAVLAEAQLSQGQIQAALSPAPLPVRA
                     LQPSSPENQARKAIAFTAVFLLYGQIIGYGIAVASGVVEEKATRIVEVLLAAVRPVQL
                     LAGKILGIGLVGLVQLFIVGGVGLAVATGTKRITLPPGATGTIALILVWFVLGYAFYS
                     CLFAAAGAIVSRQEELQNTVTPLNLVLLASFFLAFSASSNPDGTLAKVTSFLPPVAPL
                     VMPPRVAGGEVAGWEVALSVLAMLTAIAVLVPLAARIYSGAVLRTGARVKLGTAWVGG
                     RR"
     gene            complement(2125..2211)
                     /locus_tag="VEP73_01580"
                     /pseudo
     CDS             complement(2125..2211)
                     /locus_tag="VEP73_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2187..>2656)
                     /locus_tag="VEP73_01585"
     CDS             complement(2187..>2656)
                     /locus_tag="VEP73_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25164.1"
                     /translation="QRVQLAAALVHDPELLVLDEPFSGLDPVGVDVLADVLKAKAAEG
                     TPVIFSSHQLELVERLCEAVAIVNQGRLVATGGVAELRAAASHRRYEIGVAGAPAHWP
                     SASRGRPSSTRRRHASPGRAGPGRGGPTAGPCSSSTTTPTSRRSSTRHVRPDG"
BASE COUNT          342 a         1008 c          923 g          383 t
ORIGIN      
        1 atgagcgtct tcctcgtcgt gaccggcttc gcgttcaagg tgtcggccgc cccgttccac
       61 ttctgggcac cagacgccta cgagggctcc ccggtgccgg tggccgccta cctctcggtg
      121 gcgtccaagg cggccggctt cgccggcctg ctccaggtca cgttcgtcgc cttcccgtcc
      181 tacgccaggt actgggcgcc cgcgttcgcc gtgctcgccg cgttcacgat gctggtcggc
      241 aacgtgatcg ccctggccca gacgaacatg gtccgcctcc tcgcctactc gtcggtcgcc
      301 caggccggct acatgctcgt gccgttcgcg gtcggcgact cgggcgcgcc ggacgcccgc
      361 tccaaggcgt tccaggccgt cctcatctac ctgctctact acgggatcac caacctgggc
      421 gcgttcgcgg tcgtcaccct cgtcagccag cgccagcccg cgaatctcct ggccgactac
      481 gacgggctcg gccgccgctc gcccgtcttg gccggcgcca tgaccctctt cctgctctcg
      541 ctggccggct ggccgccgtt cgccggctgg tacgccaagt tcgtcgtgtt ccaggccgcg
      601 ctcggctccg ggcagcggag cctcaccgtc ctcgccgtgt tcatggcggt catgacggtg
      661 gtggcgttct tctactacct cgggatcatc gggcgcatgt ggctctcgcc tcctccggag
      721 gaggcggcgg ccaccggtgc cctcaccccg tccccgttcg ccggtctcgc catcgtgctg
      781 tccatcgccg gcgtgatcgt cctcggcgtc ctgcccggcc tcctggtcca gtacgccccc
      841 atgtccaccc tgctcgcgag caggtgagcc actgcccggg ttcgtcccgc aggcggtgga
      901 gcggaccccg accgccgccc ggcccggtgg cccggtcggg aggtcaccgg cgcccgccca
      961 cccaggcggt gcccagcttc accctggccc cggtgcgcag gaccgcgccc gagtagatcc
     1021 gcgctgccag cggcaccagg acggcgatcg ccgtcagcat ggcgagcacc gacagggcga
     1081 cctcccagcc ggccacctcg ccgccggcca cccggggcgg catgacgagc ggtgcgaccg
     1141 gcggcaggaa cgaggtcacc ttggcgagcg tgccgtccgg gttggacgag gccgagaagg
     1201 ccaggaagaa cgaggccagc aggacgaggt tgagcggcgt gacggtgttc tgcagctcct
     1261 cctgccggga gacgatcgcc ccggccgccg cgaagaggca gctgtagaag gcgtagccga
     1321 ggacgaacca gaccaggatc agcgcgatcg tcccggtcgc gcccgggggc agcgtgatcc
     1381 gcttggtccc cgtggcaacg gccagcccga ccccgccgac gatgaagagc tgcacgagcc
     1441 cgaccagccc gatccccagg atcttgccgg ccaggagctg caccgggcgc acggccgcga
     1501 gcagcacctc gacgatccgc gtcgccttct cctccacgac cccggaggcg accgcgatcc
     1561 cgtagccgat gatctgcccg tagagcagga acacggcggt gaaggcgatc gccttcctgg
     1621 cctggttctc gggactcgag ggctggagcg cgcggaccgg cagcggcgcg ggtgacaggg
     1681 ccgcctggat ctggccctgg ctgagctgcg cctcggccag gaccgcctgg gcgcgggtga
     1741 cccgcgacac cgcctgcacc aggaccccga gcttggcgtc cagttcaccc ttgctgacga
     1801 gctgcctacc gtccacgacg gcgacgtcca gtctgccgcc gcgcagctgc gcctcggcgg
     1861 tggccaggtc ggcaggttgc cgcagcctga ccttgacgcg ctcctggggt gcaagggcgg
     1921 tcagcgcctg ggcgagcggc cgcgacccgg tcccgatgag cccgacgtcg taggtcgggg
     1981 tcttgccggc gccgagcagc ttcgggacca cgatgacggc gcacaggatc gccagggtga
     2041 ccaacgtgga gatgaggaac gaccggtcgc gggtgcgctc gaccagctcg cgccgcgcga
     2101 ccaggcgaac gccgtcgaat cccgtcatga ctgcaccgcc cgcctgaaga ggtcggccag
     2161 ggtcggctcg accgcgctga agtgggtcac ccgtccggcc gcacgtgccg cgtcgaggac
     2221 ctcctgctcg tcggcgtcgt cgtcgagctg gagcacggcc cggccgtcgg ccctccccgt
     2281 cctggccccg ctcgccccgg cgaggcgtgc ctccgacggg tcgaggacgg tcgcccccgg
     2341 gaggccgacg gccagtgggc cggggcgccg gccacgccga tctcgtagcg ccggtggctg
     2401 gcggccgccc gaagctccgc gacgccgccg gtggccacca gccggccctg gttgacgatg
     2461 gcgaccgcct cgcacagccg ctcgacgagc tcgagctggt ggctcgagaa gatcaccggg
     2521 gtgccctcgg ccgccttggc cttgagcacg tcggcgagca cgtccacgcc gaccgggtcg
     2581 agcccggaga acggctcgtc gagtacgagc agctcggggt cgtggacgag ggcggccgcg
     2641 agctgcaccc gctgct
//
LOCUS       DASPYY010000108         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1001935, whole genome shotgun sequence.
ACCESSION   DASPYY010000108 DASPYY010000000
VERSION     DASPYY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001935"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VEP73_01590"
     CDS             complement(<1..527)
                     /locus_tag="VEP73_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25165.1"
                     /translation="MTAPSDPFEVVLLDLDGTVYIGHLPVPGSPEAIAALRARGQRVA
                     FLTNDPAAGRGEFAARLATIGVPATPDDVVTTGMALALLLRQEGFEGATALILGTPAF
                     RTEVMAAGLLDADATGADPKSVDLVVVGGSVDLGHAELTRATRALRAGARLFGAGRDA
                     TYPTPSGPAPASGPYL"
     gene            complement(529..1686)
                     /gene="gcvT"
                     /locus_tag="VEP73_01595"
     CDS             complement(529..1686)
                     /gene="gcvT"
                     /locus_tag="VEP73_01595"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYT25166.1"
                     /translation="MTPATDAEAGSAEAGSAAEPEAGLRRSPLDAVHRRLRAKMGAFA
                     GWDMPIEYAGTLSEHRAVREAVGVFDLSHLGKVEVRGPGAYDTLQHALSNDLDRLTEE
                     GAAQYTLCLSDDGGIVDDLIAYREADGYLLVPNAANWPAVADAVRASAHRGDTEVTPR
                     PDLAVIAVQGPAAPELLDGLFEEADGLPYMHQAPITWSGATARLCRTGYTGERGYELI
                     VPGERAPALWDELFDRGEQLGAVPCGLGARDTLRLEMGYPLHGNDISTATDPFMARLG
                     WAVRMDKPAFRGKPALLERKQSGPERLLVGLVATDRLIPRHGMAVLDPDGRPIGEVTS
                     GTFSPTLRHGIALAYVVPAFAKDGTGLLVDVRGRRCSTRVTRPPFVDAAAK"
BASE COUNT          229 a          582 c          764 g          225 t
ORIGIN      
        1 aggtaggggc cggaggcggg ggcggggccg gacggggtcg ggtaggtggc gtcgcggccg
       61 gcgccgaaca ggcgggcgcc ggcgcgcagg gcccgcgtcg cgcgggtcag ctcggcgtgg
      121 ccgaggtcga ctgagccgcc gaccaccacc aggtcgaccg acttcgggtc ggctccggtg
      181 gcgtcggcgt ccaagagccc ggcggccatg acctcggtgc ggaaggctgg cgtgccgagg
      241 atcagcgcgg tagcgccctc gaagccctcc tggcgcagca gcagggccag ggccatgcca
      301 gtggtgacca cgtcgtcggg agtggcgggg acgccgatgg tggcgaggcg ggcggcgaac
      361 tcgccacggc ccgcggccgg gtcgttggtg aggaaggcga cccgctggcc gcgcgcccgc
      421 agcgccgcga tcgcctccgg ggacccgggc accggcagat ggccgatgta gacggtccca
      481 tcgagatcga gcaggacgac ctcgaacggg tcggagggag ccgtcacgtc acttcgcggc
      541 ggcgtcgacg aagggcgggc gagtgacgcg ggtgctgcag cggcggccgc ggacgtcgac
      601 cagcagcccg gtgccgtcct tggcgaatgc ggggacgacg taggcgaggg cgatgccgtg
      661 gcgcagggtc ggcgagaagg tgccgctggt cacctcccct atcgggcggc cgtcggggtc
      721 gaggacggcc atgccgtggc gtgggatgag gcggtcggtg gcgaccaggc cgacgaggag
      781 gcgctcgggg ccggactgct tgcgctccag cagggcgggc ttgccgcgga aggcaggctt
      841 gtccatgcgg acggcccagc cgaggcgggc catgaagggg tcggtggcgg tggagatgtc
      901 gttgccgtgc agggggtagc ccatctcaag gcgcagggtg tcgcgggcgc cgaggccgca
      961 ggggaccgcg ccgagctgct caccgcggtc gaacagctcg tcccacaggg cgggcgcgcg
     1021 ctcgccggga acgatcagct cgtagccgcg ctcgccggtg tagccggtac ggcacaggcg
     1081 ggcggtcgcg ccgctccagg tgatgggcgc ctggtgcatg tagggcaggc cgtcggcctc
     1141 ctcgaacagg ccgtcgagca gttcgggggc ggccgggccc tgcaccgcga tgacggcgag
     1201 gtcggggcgt ggggtgacct cggtgtcgcc tcggtgggcg gaggcgcgca ccgcgtcggc
     1261 caccgccggc cagttggcgg cgttggggac cagcagatag ccgtcggcct cgcggtaggc
     1321 gatcaggtcg tcgacgatgc ccccgtcgtc gctgaggcag agggtgtact gggccgcgcc
     1381 ctcctcggtg aggcggtcca ggtcgttgga gagggcgtgc tgcagggtgt cgtaggcgcc
     1441 cgggccgcgg acctcgacct tgccgaggtg ggaaagatcg aagacgccca ccgcctcacg
     1501 gaccgcgcgg tgctcgctga gagtgccggc gtactcgatc ggcatgtccc agccggcgaa
     1561 cgcgcccatc ttggcgcgca ggcggcggtg gacggcgtcc agcggcgagc gccgcagccc
     1621 ggcctcgggc tccgccgctg acccggcctc ggctgaccca gcctcggcgt cggtcgccgg
     1681 tgtcactcct gctcccctgc ctcaggagcg ggaggggtcg gcggtcccgg aggggtccgg
     1741 ggaaccccag gggggtgccc aggtgaatca attctctgtg gccgggggat gcggccgggc
//
LOCUS       DASPYY010000109         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2546591, whole genome shotgun sequence.
ACCESSION   DASPYY010000109 DASPYY010000000
VERSION     DASPYY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546591"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VEP73_01600"
     CDS             complement(<1..677)
                     /locus_tag="VEP73_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYT25167.1"
                     /translation="MPARCPPEWFAGLPVLVAGLGRSGRAAATHLAEFGADVVACDDA
                     PRLEVGDLDALGVKTHLGPGSLDLLEDRRLVVVGPGLPERHPVIQAALAAGIPVWSEV
                     ELAARVARSPMVGVTGTNGKTTTTELAAAMLTATGLATVAAGNVGLPLVDAALAEPAP
                     DAIVVELSSFQLRFTESLHLAAGAWLNLAPDHLDWHPGLDAYAAAKARVWANQTRDDW
                     SLYPSDEP"
     gene            complement(727..>1683)
                     /gene="mraY"
                     /locus_tag="VEP73_01605"
     CDS             complement(727..>1683)
                     /gene="mraY"
                     /locus_tag="VEP73_01605"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYT25168.1"
                     /translation="DLPDSHQTKRGTPTMGGAVMILGAVAGYVVVHFLIEVRFTAGGV
                     MVMITMVALGTVGFVDDYLKIRNQRSLGLTKTAKFAGQAAAAVALGVGAWYWADAWRG
                     VSFVRPLAGTALPLVLFLVWVFLIVSATSNGVNLTDGMDGLASGSTVLVVSAYVVIAF
                     WQFRHSCFAPPMNAAGCYRIPSSESLDLAVAAAAMMGATAGFLWWNAPPARIFMGDTG
                     SLALGGTLAALALFTNTQLLLAVLGGLYVVETLSVILQIASFRILHRRVFKMAPIHLH
                     FELSEWPEFTVIVRFWILAGLAVAFGLGLFYAEFIAQGGTSG"
BASE COUNT          255 a          579 c          653 g          196 t
ORIGIN      
        1 ggctcgtcgc tggggtagag ggaccagtcg tcgcgggtct ggttggccca cacccgcgcc
       61 ttggcggccg cgtaggcgtc gaggccgggg tgccagtcga ggtggtcggg cgcgaggttg
      121 agccaggccc ccgcggcgag gtggaggctc tcggtgaagc ggagctggaa gctcgacagc
      181 tcgaccacga tcgcgtcggg cgcgggctcg gcgagggcgg cgtcgaccag gggcaggccg
      241 acgttgccgg ccgcgacggt ggcgaggccg gtggcggtga gcatggccgc ggccagctcg
      301 gtcgtggtcg tcttgccgtt ggtgcccgtg accccgacca tcggggacct ggcgacccgg
      361 gcggccagct cgacctcgct ccacaccggg atcccggcgg ccagcgcggc ctggatgacc
      421 gggtggcgct ctggcaggcc ggggccgacg accacgagcc gcctgtcctc gaggaggtcg
      481 agcgagcctg gcccgaggtg cgtcttgacc ccgagcgcgt cgaggtcgcc gacctcgagg
      541 cgcggggcgt cgtcgcaggc gaccacgtcc gcgccgaact cggccaggtg cgtggccgcg
      601 gcgcgcccgc tgcggccgag gccggccacg agcaccggca gccccgcgaa ccattccggc
      661 gggcaccggg cgggcatgcc gcgctcccgg cggcccgggc cgccgggccg ggtgtggcgc
      721 cggggctcag ccactggtcc ccccctgcgc gatgaactcg gcgtagaaca ggccgaggcc
      781 gaaggcgacg gccagcccgg cgaggatcca gaatcgcacg atgaccgtga actcgggcca
      841 ttccgacagc tcgaagtgga ggtggatggg ggccatcttg aacacgcggc ggtgcaggat
      901 ccggaaggag gcgatctgca ggatcaccga gagcgtctcc accacgtaca gcccgccgag
      961 caccgccagc aggagctggg tgttggtgaa cagggcgagc gcggcgaggg tgccgcccag
     1021 cgccagcgag ccggtgtcgc ccatgaagat ccgggccggc ggcgcgttcc accacaggaa
     1081 gccggcggtc gcgcccatca tggcggccgc ggcgacggcc aggtcgagcg actcgctgga
     1141 cgggatgcgg tagcacccgg ccgcgttcat gggcggcgcg aagcacgagt ggcggaactg
     1201 ccagaaggcg atcacgacgt aggcgctcac gacgaggacg gtcgacccgg aggcgagccc
     1261 gtccatcccg tcggtgaggt tcaccccatt ggaggtcgcc gacacgatga ggaacaccca
     1321 gacgaggaag agcaccagcg gcagggcggt cccggccagg gggcggacga acgagacgcc
     1381 ccgccaggcg tcggcccagt accaggcccc gacgccgagc gcgaccgcgg ccgcggcctg
     1441 cccggcgaac ttggccgtct tggtgaggcc gaggctgcgc tgattgcgga tcttcaggta
     1501 gtcgtccacg aacccgaccg tgccgagggc gaccatcgtg atcatcacca tgacgccgcc
     1561 cgcggtgaac ctcacctcga tcaggaagtg caccaccacg tagccggcga ccgcgccgag
     1621 gatcatgacc gccccgccca tcgtcggcgt gccccgcttg gtctggtggg agtcgggcag
     1681 gtc
//
LOCUS       DASPYY010000110         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_18629, whole genome shotgun sequence.
ACCESSION   DASPYY010000110 DASPYY010000000
VERSION     DASPYY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18629"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VEP73_01610"
     CDS             <1..437
                     /locus_tag="VEP73_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT25169.1"
                     /translation="ATMGDGWLTYFYTAQSFRSAWARIRDHAAEAGRDPDSLTNVAQL
                     PICVSSSFEDADRRVRAFVGAYFDVAEWSRSTPDSAIRGTPEQCAEQLAEHVAAGERH
                     IVLVPCDYALEQVERVVAEVLPALQPGRLAERRQAVGVEERT"
     gene            437..1390
                     /locus_tag="VEP73_01615"
     CDS             437..1390
                     /locus_tag="VEP73_01615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT25170.1"
                     /translation="MGGPPARQLREALEGKPRPPADKLMPAEAAVRLVRDGDHVAVGG
                     TLYSRTPMALLFELLRQGRSDLTVSRPLTCYEAELFLVGGLAGGIVTSWVGIGLPWGV
                     SRVVRELVEGGRATYEEWSHLALGLRYKAGAMGVPFLPTFTMLGSDLVGLVDTHELTC
                     PFTGEKVLLVPALNPDVALLHVHRADRSGNAQIDGYRHMDADMAAAARTVIVSTEEVV
                     DAERIAERPDATVLPHFQVDAVVEAPMGAFPHECYGRYEADFAHFDDYAADVRRDGVD
                     AVSAYLDRHVRRQPDFAAFLASFGTSRLQGQQARAAELMAR"
     gene            1411..2148
                     /locus_tag="VEP73_01620"
     CDS             1411..2148
                     /locus_tag="VEP73_01620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT25171.1"
                     /translation="MTVSAGRLLRDHRVVFAGVGVPLLASVLAKQRHAPDLTIVLEGG
                     IIGPRMLAGRLPISTNEMRAARHAPMLTAITDVFLLAQRGFLHYGFLGAAQIDMHGNI
                     NTSVIGPVERPRVRLPGTGGANDIASLCNEVLVVTPHEPRRFVERVDFVTSPGHLGGG
                     DAREKAGLVSRGPSTVVTDLALLDFEPESRRMRLRALQPGVTVEDVRASTGFELPVHP
                     EVCELEPPAIPDLEILRELDAGAAARD"
     gene            2159..2863
                     /locus_tag="VEP73_01625"
     CDS             2159..2863
                     /locus_tag="VEP73_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYT25172.1"
                     /translation="MGIVETRWRVGLLVPSSNTVMEPDLWRALPPGATLHTGRMYLEE
                     TTPEGESRMLDEHVLPAARDLATARPDLVVFGCTSAGALRGDGYDGELCARIGELSGV
                     PTVSVIASVRQAIAASGARRVGVVTPYVDALNQRIRESLEAGGVEVAGIAGLGISDNF
                     EIARVPAERICDFAERTLGGLAVDLAFVSCTNFPAVSALPELERRLGLPVVTSNQAAI
                     AAVLALLDAAPALRGG"
     gene            2867..3781
                     /locus_tag="VEP73_01630"
     CDS             2867..3781
                     /locus_tag="VEP73_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT25173.1"
                     /translation="MVAAPFRYERVPSFEEAVRALHERGEDAKVIAGGQSLVPMMNLR
                     IARPEWLIDLNPVGAGPIEDHGGYLRLPALTRHRAIEEAEVVRRRCPILAEAAAHVGN
                     VRTRTRGTIGGSLAHADPAAELPCVAVALGAEVTVLGREGARAVAADDLLVSYLTTTL
                     APDEVVVEVRVPTLGPRSGWSFLEMSRRSCDWMVVGVAALVELDAAGAVTAASVAIGG
                     VAERAWLVPAEALADLLGAAPEEALLARVAERAAAMLRPEDDVQASGEYRRRLAAVLG
                     RRALAEAVARAGLPEDEPAPRGNGERAP"
     gene            3778..>4327
                     /locus_tag="VEP73_01635"
     CDS             3778..>4327
                     /locus_tag="VEP73_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT25174.1"
                     /translation="MSEPGRQRVAFTVNGEDREAWVFPHESLLDVLRGALGHHEVRYG
                     CGEGVCGTCTVLLDGEPVSGCLLLAVQADGCSLLTVRGLAPGGDLHPLQACFLRNGAA
                     QCGFCTPGMLLTAWALLQRDPRPSRERIRRELVGNLCRCTGYSKILDAVEEHAAEGSG
                     EPRRGAPVGREGSGEPRRGAPVG"
BASE COUNT          532 a         1606 c         1619 g          570 t
ORIGIN      
        1 tggcgaccat gggcgacggc tggctgacgt acttctacac ggcgcagagc ttccgctcgg
       61 cctgggcgag gatccgcgac cacgccgccg aggcgggccg tgaccccgac tcgctcacga
      121 acgtcgccca gctgccgatc tgcgtctcgt cctccttcga ggacgccgac cgccgcgtac
      181 gggcgttcgt cggtgcgtac ttcgacgtcg ccgagtggag ccggtccacg cccgacagcg
      241 ccatccgcgg cacgcccgag cagtgcgccg agcagctcgc cgagcacgtg gcggccggcg
      301 agcgccacat cgtgctggtg ccctgcgact acgcgctcga gcaggtcgag cgcgtcgtcg
      361 ccgaggtcct gcccgcgctc cagccgggcc ggttggccga gcgccgccag gccgtgggcg
      421 tggaggagcg gacctgatgg gcggcccgcc ggccaggcag ctccgcgagg cgctcgaagg
      481 caagccgcgc ccgcccgccg acaagctgat gcccgccgag gccgccgtgc ggctggtccg
      541 cgacggcgac cacgtcgccg tcggcggcac cctgtactcc cgcacgccca tggcgctgct
      601 gttcgagctg ctgcgccagg gccgcagcga cctcacggtc tcccgtccgc tcacctgcta
      661 cgaggcggag ctgttcctcg tcggcgggct ggctggcggc atcgtgacta gctgggtcgg
      721 gatcggcctg ccctggggcg tctcgcgggt cgtgcgcgag ctggtggagg ggggccgggc
      781 gacctacgag gagtggagcc acctggcgct cgggctgcgc tacaaggccg gcgcgatggg
      841 cgtgccgttc ctccccacct tcacgatgct cggctccgac ctcgtcgggc tcgtcgacac
      901 ccacgagctc acctgcccgt tcaccgggga gaaggtgctg ctggtcccgg cgctgaaccc
      961 cgacgtcgcc ctgctgcacg tgcaccgcgc cgaccggtcc ggcaacgccc agatcgacgg
     1021 ctacaggcac atggacgcgg acatggcggc ggcggcccgc acggtgatcg tgagcaccga
     1081 ggaggtggtc gacgccgagc gcatcgcgga acgtccggac gcgaccgtgc tcccccactt
     1141 ccaggtggac gcggtggtcg aggcgccgat gggcgccttc ccgcacgagt gctacggccg
     1201 ctacgaagcg gacttcgcgc acttcgatga ctacgccgcc gacgtcaggc gcgacggcgt
     1261 cgatgcggtc tccgcctacc tggatcgcca cgtccgtcgc caacccgact tcgccgcctt
     1321 cctggcgagc ttcggcacgt cccggctgca gggccagcag gcacgcgccg cggagctgat
     1381 ggcgcggtga gctggacgcc gcaggagctg atgacggtct cggcgggccg cctcctcagg
     1441 gaccatcggg tggtgttcgc cggggtgggc gtgccgctgc tcgcctcggt gctcgccaag
     1501 cagcggcacg cgcccgacct caccatcgtg ctcgagggcg gcatcatcgg cccgcgcatg
     1561 ctcgcgggca ggctgccgat ctccaccaac gagatgcgcg ccgcacggca cgcccccatg
     1621 ctcaccgcca tcaccgacgt cttcctgctg gcgcagcgcg gcttcctcca ctacggcttc
     1681 ctcggggccg cccagatcga catgcacggc aacatcaaca ccagcgtcat cggcccggtc
     1741 gagcgcccgc gcgtccgcct gcccggcacg ggaggggcca acgacatcgc ctcactctgc
     1801 aacgaggtac tggtggtcac cccccacgag ccgcgccgct tcgtcgagcg ggtcgacttc
     1861 gtgaccagcc ccggccacct cggcggcggc gacgcccgcg agaaggccgg cctggtctcc
     1921 cgcgggccga gcacggtcgt caccgacctg gcgctgctcg acttcgagcc ggagagcagg
     1981 cgcatgcgcc tccgcgcgct gcagcccggc gtcaccgtgg aggacgtccg cgccagcacc
     2041 ggcttcgagc tgccggtcca cccggaggtg tgcgagctgg agccaccggc gataccagat
     2101 ctcgagatcc tgcgggagct cgacgccggt gccgcggcgc gggactgagg agcggagcat
     2161 gggaatcgtc gagaccaggt ggcgggtcgg actgctcgtc ccgtcctcga acaccgtgat
     2221 ggagccggac ctctggcgtg cgctgccgcc cggcgccacg ctgcacacgg gccgcatgta
     2281 cctcgaggaa accacccccg agggcgagag ccggatgctc gacgagcacg tgctcccggc
     2341 cgcgcgcgac ctcgccaccg cccggcccga cctggtcgtc ttcggctgca ccagcgccgg
     2401 tgcgctgcgc ggcgacggct acgacggcga gctgtgcgcg cgcatcggcg agctgtccgg
     2461 ggtgcccacc gtcagcgtga tcgcctcggt caggcaggcg atcgcggcga gcggcgcgcg
     2521 ccgcgtcggc gtggtcaccc cctacgtcga cgcgctcaac cagcgcatcc gggagagcct
     2581 ggaggccggc ggcgtcgagg tggcggggat cgcggggctc gggatctccg acaacttcga
     2641 gatcgccagg gtcccggcgg agcggatctg cgacttcgcc gagcggacgc tgggcgggct
     2701 cgccgtcgac ctggcgttcg tgtcctgcac caacttcccg gcggtctcgg ccctgcccga
     2761 gctggagcgg cgcctcggcc tgccggtggt caccagcaac caggcggcca tcgccgcggt
     2821 gctcgcgctg ctggacgcgg ccccggcgct ccggggtggg tgaccggtgg tcgccgcgcc
     2881 cttccgctac gagcgtgtgc cctccttcga ggaggccgtg cgggcgctgc acgagcgggg
     2941 cgaggacgcc aaggtcatcg ccggcggcca gagcctggtc ccgatgatga acctccggat
     3001 cgcccggccc gagtggctga tcgacctcaa cccggtcggt gccggcccga tcgaggacca
     3061 cggcgggtac ctgcgcctcc ccgcgctcac gcggcaccgc gccatcgagg aggccgaggt
     3121 ggtgcggcgg cgctgcccga tcctggccga ggcggcggcg cacgtcggca acgtgcgcac
     3181 ccggacccgc ggcacgatcg gcggcagcct cgcccatgcc gacccggccg ccgagctgcc
     3241 ctgcgtggcg gtcgcgctgg gcgcggaggt caccgtgctc gggcgtgagg gcgcccgcgc
     3301 ggtggcagcc gacgacctgc tggtgtcgta cctgaccacc acgctcgccc ccgacgaggt
     3361 cgtcgtcgag gtgcgcgtgc ccaccctcgg gccgcggagc ggctggtcgt tccttgagat
     3421 gagccgccgc agctgcgact ggatggtggt cggtgtggcg gccctggtcg agctggacgc
     3481 cgcgggtgcg gtgacggccg cctcggtcgc gatcggcggc gtggccgagc gtgcctggct
     3541 ggtcccggcg gaggcgctgg cggacctgct cggtgcggca ccggaggagg cgctgctcgc
     3601 gcgggtggcg gagcgggccg ccgccatgct ccgtcccgag gacgacgtgc aggcctcggg
     3661 ggagtaccgg cggcgcctgg ccgccgtcct cggccgccgg gcgctggccg aggcggtggc
     3721 ccgggccggg ctgccagagg acgagccggc gccgcgcggg aacggggagc gggctccatg
     3781 agcgagccgg gcaggcagcg cgtcgccttc acggtcaacg gcgaggaccg ggaggcctgg
     3841 gtgttccccc acgagagcct cctcgacgtg ctcaggggcg ccctcggcca ccacgaggtc
     3901 cgctacggct gcggcgaggg ggtctgcggg acctgcacgg tcctgctcga cggcgagccg
     3961 gtcagcggct gcctgctgct ggcggtgcag gccgacggct gctcgctcct gaccgtgcgg
     4021 ggcctcgccc ctggcggcga cctgcacccc ctgcaggcgt gcttcctgcg caacggcgcc
     4081 gcgcagtgcg ggttctgcac gcccggcatg ctgctcaccg cctgggcgct cctgcagcgc
     4141 gacccgcggc cgagccgcga gcggatccgc cgggagctgg tcggcaacct gtgccgctgc
     4201 accggctaca gcaagatcct ggacgcggtc gaagagcacg ccgcggaggg gtccggggaa
     4261 ccccggaggg gtgccccggt gggtcgggag gggtccgggg aaccccgacg gggtgccccg
     4321 gtcggac
//
LOCUS       DASPYY010000111         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2406380, whole genome shotgun sequence.
ACCESSION   DASPYY010000111 DASPYY010000000
VERSION     DASPYY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406380"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEP73_01640"
     CDS             <1..358
                     /locus_tag="VEP73_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYT25175.1"
                     /translation="LGETPSDMVGALVEDVIRHSRGQPRAVQGDGCAEAMATLRAFMF
                     DRVYLRPEARRQTERAERLLTDLYGHYLEHPDAMPTGASLASDPVEQQAVDAIAGMTD
                     RYALAAWNAAFAPTPD"
     gene            543..989
                     /locus_tag="VEP73_01645"
     CDS             543..989
                     /locus_tag="VEP73_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HYT25176.1"
                     /translation="MREKVVMSTPAPQGAGGYRVGDAERGRTVDLLKEAHAAGYLTLD
                     EVDERLTLALTARSDRDLARLAADLPPDWRARHEVAPRPQQPAPRRPAPPPMVWALVP
                     LLLAAVVMLVIGFATRGFFFPWPLLWLWFFLGRRWTRASWHHTYRL"
     gene            1189..>1741
                     /locus_tag="VEP73_01650"
     CDS             1189..>1741
                     /locus_tag="VEP73_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYT25177.1"
                     /translation="MPEWWHGGRRGRFGPGAAEWAAMAGGRGRRGPGPFGFGRGPRGG
                     FFFGGPPFGGPPFGPPPFMRGGRAGRGNVRAAVLALLAEQPLHGYQIIQEISERSGGL
                     WRPSPGSVYPAVQQLEDEGLVRIEQVEGRRMIHLTDAGEAYVREHADELDAPWEAVAG
                     GVSDGVLELHDLLGQVGAAVVQVA"
BASE COUNT          223 a          636 c          657 g          225 t
ORIGIN      
        1 gctcggcgag accccctcgg acatggtcgg cgccctggtg gaggacgtga tccgccattc
       61 gcgcggccag ccccgcgccg tccagggcga cggctgcgcc gaggcgatgg ccaccctgcg
      121 cgcgttcatg ttcgaccgcg tgtacctgcg gcccgaggcc agacggcaga ccgagcgcgc
      181 cgagcgcctg ctcaccgact tgtacgggca ctacctcgag cacccggacg cgatgccgac
      241 gggggcgagc ctcgcgtccg acccggtcga gcagcaggcg gtggacgcga tcgcggggat
      301 gaccgaccgc tacgcgctcg cggcgtggaa cgccgccttc gcgccgacgc ccgactgagc
      361 tgtcgtcacg ccggccagga cgccctcgcc atgcaggccg ccagggtgtt ccttcccggc
      421 ggggacgaga tggtggagga acggcggaac cgccgccacc accccggttg acgaccgtca
      481 ttacacactt acactggaag gggagagcag tgaacggtcg ggccggacgg agccggcccc
      541 acgtgagaga gaaggtcgtc atgagcaccc ccgcgcccca gggggccggc ggttaccggg
      601 tcggcgacgc cgagcggggc cgcacggtcg acctgctcaa ggaggcgcac gccgccggct
      661 acctcaccct cgacgaggtc gacgagcggc tcacgctggc gctcacggcg cgcagcgacc
      721 gcgacctcgc ccggctcgcg gccgacctgc cgccggactg gagggccagg cacgaggtcg
      781 cgccgcgacc gcagcagccg gcgccccgcc gccccgcccc gccccccatg gtgtgggcgc
      841 tggtgcccct cctgctcgcc gcggtggtga tgctcgtgat cgggttcgcc acgcgcggct
      901 tcttcttccc gtggccgctc ctgtggctct ggttcttcct cgggcgccgc tggacgcggg
      961 cttcctggca ccacacctac cggctgtagc tccctggacg gtttccccgc cggggggcga
     1021 cgacccggtc ggtcccgcgg tgccccgctc gttccctcgg atcgggcaga tcgggccgtg
     1081 attcccttgc gctcgatgcg tcgacgatat atcgtagacg catcgagacg gtgtgcgcgg
     1141 aaggagcacc acatgcacgg agcacatgga gcacacggag cacacgacat gccggagtgg
     1201 tggcacggcg gccgccgtgg ccgcttcggg cctggcgctg ccgaatgggc cgcgatggcc
     1261 ggcgggcggg ggcgccgcgg ccctgggccg ttcggcttcg ggcgcgggcc gcggggcggg
     1321 ttcttcttcg gcgggccgcc gttcggcggg cccccgttcg gcccccctcc cttcatgcgc
     1381 ggcggcaggg cgggacgcgg gaacgtccgc gccgcggtgc tggccctgct ggccgagcag
     1441 ccgctgcacg gctaccagat catccaggag atcagcgagc gcagcggggg cctgtggcgc
     1501 ccgagcccgg gctcggtcta cccggcggtg cagcagctcg aggacgaggg gctcgtacgg
     1561 atcgagcagg tcgaggggcg gcgcatgatc cacctcaccg acgccgggga ggcctacgtg
     1621 cgcgagcacg cggacgagct cgacgcgcca tgggaggccg tggccggcgg ggtcagcgac
     1681 ggggtgctcg agctgcacga cctgctcggg caggtcggcg ccgccgtcgt ccaggtggcg
     1741 c
//
LOCUS       DASPYY010000112         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2687051, whole genome shotgun sequence.
ACCESSION   DASPYY010000112 DASPYY010000000
VERSION     DASPYY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687051"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VEP73_01655"
     CDS             <1..1521
                     /locus_tag="VEP73_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HYT25178.1"
                     /translation="GRRSGGREGRRAARLHAVVERVPFITRTMAPFEVLSEEGLALIE
                     HNADVILEQVGVEFRDADALATLKDAGAEVDGELVRFPRGMARALVQATAPREFTQHA
                     RNPANDVRIGGDATVFAPAYGPPFVRDLDQGRRYGTIEDFRNFVKLTYASPWLHHSGG
                     TVCEPVDLPVNKRHFDMVEAHLRLSDKPFMGSVTHPDRASDTVAMARIVFGAERLERD
                     CVILSLINANSPLVWDATMLGAARVYAEANQATLMTPFILAGAMSPATVAGTAAQTLA
                     EALAGMAYVQLVRPGAPVVFGSFASSMSMQSGAPTFGTPEPALVLYALAACARRLGVP
                     FRSGGALTAAKVPDAQAAFESANTMQPTMLGGVNFVLHVAGWLEGGLCMGYEKFVLDN
                     DQAGAWHAFARGVDLSPNGQALDAIIANGPGQHFLGSAHTLANFETAFWRSQVADANS
                     FEQWDLEGSRDAAQRANALWKKTLADYQPPPIDDAVDQELREWIERRKASFPDSNV"
     gene            complement(1525..2022)
                     /locus_tag="VEP73_01660"
     CDS             complement(1525..2022)
                     /locus_tag="VEP73_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HYT25179.1"
                     /translation="MADVSMVEVPTLSQVSLRVDLTPAQGRPAPPLELPLQPNNWRRI
                     GILEALWLGPDEWLVTAGQGNTGKMVILLEAAFDGRPHSVVDVSANRIAIDLTGPDRL
                     DLLATGCPLDLHPRVWNEGACAQTLLARVPVLLQERAAGTRLFVRPSFAAWLTDWLDA
                     AAAVS"
     gene            complement(2015..>4432)
                     /locus_tag="VEP73_01665"
     CDS             complement(2015..>4432)
                     /locus_tag="VEP73_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HYT25180.1"
                     /translation="DPGTQARAARPRHAHVETLVVGGGVAGLRAAREAAERGDRVLLL
                     DERSWLGGTARSFEQVEGRPAWEWVEETAAALRSTPDVTLLTEAAALGLYDAGYAVIH
                     ERSRPVEHLWRVRATRVVLATGAHERPVAFAGNDRPGVMLSGAVLTYLDRFGVLPAER
                     AVVWVANDAGYATARALRAAGAEVVAVVDARREGEAAAQDQARADGLEVLAGAVIVAT
                     DAATGSGHVSGAFAVGPDGQRIRLEAELLAVSGGWNPVVQLHRAIGGGLRYDPARACF
                     VPDGGPTWLSATGAAAGEVPAAEPVWHIAAGDGDSSALSTSLSEHFVDLQRDQTVSDV
                     AEALDAGLRSVEHIKRVTHIGTAADQGRTSGTLAAEIVNQLLGADPGAQGPTGARPPW
                     APLPYHVLAGPFRGRLFDPVRETPIHPWHVAHGAVFENVGQWKRPWYFPREGESMEEA
                     VLRECAAARDGVALMDASTLGKIEVVGPDAATFLDRMYTNAISTLPVGRLRYGVMLGL
                     DGMVFDDGVAMRLAADRYLVTTTTGNAAAVLDRFEEWLQTEWPELRCWATSVTEQWAT
                     IAVVGPLAREVLWAAGTDVDVSTAAFPFTAWRDGTVAGVPARVTRVSFSGSLAFEVNI
                     ASWHGLQVWEALYAAGQPFGLTVYGTEAMHVLRAEKAFPIVGQDTDGTVTADDLGMSW
                     IVNLRKGDFVGRRSLRRADLTRPNRKQLVGLLPQDTAALIPEGAQLIDPGSVGASPPV
                     PMRGHVTSSYRSAALGRTFALALLEAGRQRHGDTVFAFDARLTEPIAATVADPVFYDP
                     EGARRDG"
BASE COUNT          610 a         1639 c         1600 g          583 t
ORIGIN      
        1 gggcggcgca gcggcgggcg cgaggggcgc cgcgccgcac ggctgcacgc ggtcgtcgag
       61 agggtcccgt tcatcacccg cacgatggcg ccgttcgagg tcctttccga ggagggcctc
      121 gccctcatcg agcacaacgc cgacgtgatc ctggagcagg tcggcgtcga gttccgcgac
      181 gccgacgcgc tcgccaccct caaggacgcc ggcgccgagg tggacggcga gctggtccgc
      241 tttccccgcg gcatggcccg cgcgctggtg caggcgaccg cgccccgcga gttcacccag
      301 cacgcccgca accctgccaa cgacgtgcgc atcggcgggg acgccacggt gttcgccccc
      361 gcctatgggc cgccgttcgt ccgcgacctg gaccagggcc gccgctacgg gaccattgag
      421 gacttccgca acttcgtgaa gctcacctac gcctcgccct ggctgcacca cagcggcggg
      481 accgtctgcg agccggtcga cctgccggtc aacaagcgcc acttcgacat ggtcgaggca
      541 cacctgcgcc tctccgacaa gccgttcatg ggctcggtca cccaccccga ccgcgccagc
      601 gacaccgtgg cgatggcccg gatcgtgttc ggcgccgagc ggctggagcg cgactgcgtg
      661 atcctcagcc tgatcaacgc gaattccccg ctggtctggg acgccaccat gctcggcgcc
      721 gcccgcgtct acgccgaggc caatcaggcc accctgatga ccccgttcat cctggcggga
      781 gcaatgagcc cggcgaccgt ggcggggacc gccgcccaga ccctcgccga ggcgctggcc
      841 ggcatggcct acgtgcagct ggtgcggccg ggcgccccgg tggtgttcgg ctcgttcgcc
      901 tcctcgatgt cgatgcagtc cggcgcgccc accttcggta cccccgagcc cgccctggtg
      961 ctctacgccc tggccgcctg cgcgcgccgg ctcggcgtcc cgttccgcag cggcggcgcg
     1021 ctcaccgccg ccaaggtccc cgacgcgcag gctgccttcg agtcggccaa caccatgcag
     1081 ccgaccatgc tcggcggcgt gaacttcgtg ctgcacgtgg ccggttggct ggagggcggc
     1141 ctctgcatgg gctacgagaa gttcgtgctc gacaacgacc aggccggcgc ctggcacgcc
     1201 ttcgcccggg gcgtggacct ttccccgaac ggccaggcct tggacgcgat catcgccaac
     1261 ggtcccggcc agcatttcct cggctccgcc cataccctgg ccaacttcga gaccgccttt
     1321 tggcgctccc aggtcgccga cgccaactcc ttcgagcaat gggacctgga gggctcccgc
     1381 gacgccgccc aacgcgccaa cgccctctgg aaaaagaccc tcgccgacta ccagccccct
     1441 ccaatcgacg acgccgtcga ccaggagctg cgcgagtgga tcgaacgccg caaagcctcg
     1501 ttccccgact caaacgtctg acggctacga gaccgcggca gcggcgtcca gccagtcggt
     1561 cagccaggct gcgaacgacg ggcgcacgaa caggcgggtc ccggcggcgc gttcctggag
     1621 caggacgggc acgcgggcca gcagggtctg ggcgcaggcg ccctcattcc agacacgggg
     1681 gtgcaggtcg agggggcagc cggtcgccag caggtcgagc cggtcggggc cggtcaggtc
     1741 gatggcgatg cggttggcgc tgacgtcgac cacggagtgg ggccttccgt cgaaggcggc
     1801 ctccagcagg atcaccatct ttccggtgtt gccttgaccg gcggtcacca gccattcgtc
     1861 ggggccgagc catagggcct ccaggatccc aatccgccgc caattgttcg gctggagcgg
     1921 gagctcgagc ggtggtgcag gccggccctg cgctggtgtc aggtctaccc gcaggctcac
     1981 ctgggagagg gttgggacct ccaccatgct gacgtcagcc atcgcggcgt gctccctccg
     2041 ggtcgtagaa cactgggtcg gcgacggttg cggcgatcgg ttcggtgagg cgggcgtcga
     2101 acgcgaagac ggtgtcgccg tggcgttgcc ggccggcttc gagcagcgcg agcgcgaagg
     2161 tccggccgag cgcggcgctg cggtaggacg aggtcacatg gccccgcatc ggcacgggtg
     2221 gtgaggcgcc cacggagccg gggtcgatca gctgggcgcc ctccggaatc aatgcagcgg
     2281 tgtcctgggg gagcaggccg acgagctgtt tgcggttcgg ccgggtcagg tcggcgcggc
     2341 ggagcgagcg gcggccgacg aagtcgccct tgcggaggtt gacgatccag gacatgccga
     2401 ggtcgtcggc ggtgacggtg ccgtcggtgt cctggccgac gatcgggaag gccttctcgg
     2461 cgcggaggac gtgcatggcc tcggtgccgt agacggtcaa gccgaacggc tgcccggcgg
     2521 cgtacagggc ctcccagacc tggaggccgt gccagctggc gatgttgacc tcgaaggcga
     2581 ggctgcccga gaacgacacc cgggtgaccc gggccggcac gccggcgacg gtgccgtcgc
     2641 gccaggccgt gaacgggaac gccgcggtgc tgacgtccac gtcggtgccg gcggcccaca
     2701 ggacctcacg ggccaggggg ccgacgacgg cgatcgtggc ccactgctcg gtcacgctgg
     2761 tcgcccagca acgcagctcg ggccactcgg tctggagcca ctcctcgaag cggtcgagca
     2821 ccgccgccgc gttgccggtg gtggtcgtga ccaggtagcg gtcggcggcc agccgcatcg
     2881 ccacgccatc gtcgaagacc atcccgtcca ggccgagcat gaccccgtag cggagccgcc
     2941 cgaccggcag ggtggagatc gcgttggtgt acatccggtc gagaaaggtc gcggcgtccg
     3001 ggccgaccac ctcgatcttt ccgagggtcg aggcgtccat cagggcgacg ccgtcgcggg
     3061 ccgccgcgca ctcgcgcagc accgcctcct ccatcgactc gccctcgcgc gggaagtacc
     3121 agggacgctt ccactgcccg acgttctcga acaccgcgcc gtgggccacg tgccagggat
     3181 ggatcggggt ctcgcggacc gggtcgaaca gccggccccg gaacggcccg gccagcacgt
     3241 ggtaggggag cggcgcccac ggcggccggg ctcccgtcgg gccctgcgcg ccggggtcgg
     3301 cgccgaggag ctggttgacg atctcggcgg cgagcgtccc cgaggtgcgg ccctggtcgg
     3361 cggcggtgcc gatgtgggtc acccgcttga tgtgctcgac gctgcgcagg cccgcgtcga
     3421 gggcctcggc gacgtcggag accgtctggt cgcgctgcag gtcgacgaag tgttccgaga
     3481 gagaggtgga gagggcgctg gagtccccgt ccccagcggc gatgtgccag accggctcgg
     3541 cggcgggaac ctccccagcg gcggcgccgg tcgccgacag ccaggtgggt ccgccgtcgg
     3601 gtacgaagca ggcgcgggcg gggtcgtagc gaaggccgcc gccgatcgcc cggtggagct
     3661 gcaccaccgg gttccagcca cccgagacgg ccagcagctc ggcctccagg cggatccgct
     3721 gcccgtcggg tccgaccgcg aacgccccgc tgacgtgccc ggagccggtg gcggcgtcgg
     3781 tggcgacgat cacggccccg gccagcacct ccaggccgtc ggcgcgggcc tggtcctggg
     3841 cggcggcctc gccttcgcgc cgggcgtcga ccaccgcgac cacctcggca cccgccgccc
     3901 ggagcgcccg cgcggtcgcg tagccagcgt cgttggcgac ccaaaccacc gcccgctccg
     3961 ccgggagcac cccgaaccgg tcgagatagg tgagcaccgc cccggacagc atcacccccg
     4021 gccggtcgtt gccggcgaac gcgaccggcc gctcgtgtgc gccggtcgcc aggaccaccc
     4081 gcgtcgcgcg cacgcgccag agatgttcga ccgggcggga gcgctcgtgg atgaccgcgt
     4141 agccagcgtc gtagaggccg agcgcggcgg cctcggtcag cagcgtcacg tccggcgtgc
     4201 tccgcagcgc ggcggcggtc tcctcgaccc actcccaggc cggccgcccc tccacctgct
     4261 cgaaggagcg ggcagtcccg ccgagccagg accgctcgtc caacagcagg acccggtcgc
     4321 cgcgctccgc ggcctcccgc gcggcccgca gccccgcgac cccgccgccg acgaccagcg
     4381 tctcgacgtg ggcatggcgc ggtcgcgccg cccgggcctg cgtcccgggg tc
//
LOCUS       DASPYY010000113         6322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2406810, whole genome shotgun sequence.
ACCESSION   DASPYY010000113 DASPYY010000000
VERSION     DASPYY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406810"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VEP73_01670"
     CDS             complement(<1..1357)
                     /locus_tag="VEP73_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYT25181.1"
                     /translation="MSPARSVPGSAIPGFGGTVIRPDQPGYDAARAVWNAMHDRRPAL
                     IARPRSAPDVAAAIGFARAEGLPIAVRGGGHSMPGHSTCDGGMVIDLRELSRVTVDPV
                     TRRATVGGGALLGDVDRATQRHGLVVPAGVVSHTGVGGLTLGGGVGRLMRRFGLTVDS
                     LLSAEVVTADGRILRASAGQHPDLFWALRGGGGNFGVVTEFEFALHELSELVILATFH
                     PLDQARRVIGQGRREMADPAARDELLWTSFLRRASEVPWMPPDLVGRHGIMSLVEWSG
                     DAEEGRAVLAKIRDELQPAASDLSVVPFLVMQTITDDLFAHGLRTYIKAGFAADLTDG
                     LVDALIGRAALLRSPVSQVELLALGGAIARVHPDATAFPFRQPRWLINIPATWRDAAD
                     DEREIGWARATYAAVRPYLAEGSYVNFMGDDEDDSAAAAYGRTIERLQRVKARYDPDN
                     TF"
     gene            complement(1354..1872)
                     /locus_tag="VEP73_01675"
     CDS             complement(1354..1872)
                     /locus_tag="VEP73_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT25182.1"
                     /translation="MYPLHHVGYWVDDLDAAMKQWHRDLDVGPFDVIEHVPFEEFVLT
                     DGGQARADVVFDHSAAFAAWGPIVVELGQVHAADDRLAAAYRIAPGAISHVSWVVPDL
                     AEESARLARLGCRLINTARTGPVSVAWHEGGPLFPHPVEVHQHNDVIAGMHARLCALR
                     VPGDRSPADGAA"
     gene            complement(1872..3092)
                     /locus_tag="VEP73_01680"
     CDS             complement(1872..3092)
                     /locus_tag="VEP73_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT25183.1"
                     /translation="MSTAKPLHVLIAGGGLSGLALAQGLIKDGHTCEVFERDADDTRK
                     IGYYLHMNADGGEALRRCLSADLFELYAQTSRRTYDRRESVVLDDQLNELSSQPHLGP
                     PNEGERPHTGVHRRTLRAILRARLGDAFHPGQPVTGYEETPGQVTVTLANGNTAQGDV
                     LVGADGIRSAVRGQKLPGTTVIDTGVRGLGVFGRTPLTPELAAQLPPNLFEGVIIAAD
                     RKGSRLLIAVYRPRQRPQDAAAAIAPDVTLEPVEDYVMISCSVPPGTVIPPAAQWTDG
                     TPAMLRDAMLAAIEGWHPAARALVAGVDLASVFVIPFGFLEPAPPWEPSRVTLVGDAA
                     HAMLPTLGLGANLALRDAAHLLGQLSAAARGEAALVPAIGAYEQGMRDYVYPFMRMTS
                     EHDKQFGGGALEGR"
     gene            complement(3097..3987)
                     /locus_tag="VEP73_01685"
     CDS             complement(3097..3987)
                     /locus_tag="VEP73_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT25184.1"
                     /translation="MKLVTFDAGRVGRLEGEGDDAVVVELDCASTREWFERGGQAGET
                     GERLPLAQVRLRAPIVPKKFFHTAGNFADHHEELQAVDWSHPVHKGIVFFQNVDAIIG
                     PDDAIVYPEGLTKELDYELELAIIIGKAGKFFGPQEAAGYVAGYTVFNDITARDIQRR
                     EMQSGVFSFSKAIDTFCPIGPWIVTADEVPDAQALAMELRVNGGVRQRGNTARMLISI
                     PHLVAYHSAQGYSAGDILTTGTISGVAAVQPNPFEFYLQPGDSIEAEVEGVGVLRNHV
                     VPWAAAHSTPPYSTDLYSAD"
     gene            complement(4010..4678)
                     /locus_tag="VEP73_01690"
     CDS             complement(4010..4678)
                     /locus_tag="VEP73_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYT25185.1"
                     /translation="MTSGARIGKFEPSALDAEQRSLYDAIAGGRRAQGPQLFRLADAD
                     GRLEGPFNAFLLQPRLGAALQALGASVRYETGLDDRCREIAILVVAAHWRSDFEWHAH
                     EAVGRAAGLGDAELAALREGRHADLAGRESVVARTTAALVTRGDLDDAEYGEAVGHLG
                     PAGLFELLTLVGYYATLALQLRVFRVPAPVDTAPRGHCPRGTRRPGLTPPPLAHEDGR
                     KLLS"
     gene            complement(4675..5439)
                     /locus_tag="VEP73_01695"
     CDS             complement(4675..5439)
                     /locus_tag="VEP73_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT25186.1"
                     /translation="MEALAKGLRILSLFDEQRPSWRVSDLAAAAGLPMPTVYRVVMTL
                     ASEGYLDHLPNGDYRPGVRTLTLGTAALRSLDLVAIATPKLTQLGERTGETVNLAVLS
                     GDRVLYLIRLRNSDLVTANIQVGSTLPAVHTSIGKLLLAHLDEADLQSRITDASFAAN
                     SGPNAKVSLAELRDELRTIRDQGWAMQDEELAYGLRSVAAPITAPDGRVLAGVNLAVQ
                     AQDWSTQRIIRELRPAVLATCAEISALLSDTTAGSL"
     gene            5731..>6322
                     /locus_tag="VEP73_01700"
     CDS             5731..>6322
                     /locus_tag="VEP73_01700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT25187.1"
                     /translation="MQDWLTLDRRPTLVFGAGGLGGASALSLASQGARVALVDASQEN
                     LDTVVRAAKEAGREVKPLVADLRTAEACRAAVGEAVALVGTPQVFLHAVGRNVRKPIL
                     ELADDDWQETLSLNLSSAYWLGQAVGRLMVERRYGRMVFVSSVSGLLAHPYHGPYAAT
                     KGGMNQMLRVMAREWARHGVTVNAVAPGYIETDLTKD"
BASE COUNT          859 a         2201 c         2411 g          851 t
ORIGIN      
        1 ggaaggtgtt gtccgggtcg tagcgcgcct tgacccgctg gaggcgctcg atcgtgcgcc
       61 cgtaggcggc ggccgccgag tcgtcctcgt cgtcgcccat gaagttgacg tagctgccct
      121 cggccaggta gggccggacc gcggcgtagg tcgcccgggc ccagccgatc tcgcgctcgt
      181 cgtcggcggc gtcccgccag gtggccggga tgttgatcag ccagcgcggc tggcggaacg
      241 ggaacgcggt cgcgtccggg tgcacgcggg cgatcgcgcc gcccagcgcc agcagttcca
      301 cctggctcac cggcgaccgg agcagggccg cgcggccgat cagcgcgtcg acgaggccgt
      361 cggtcaggtc ggcggcaaag ccggccttga tgtaggtgcg caggccgtgg gcgaacaggt
      421 cgtcggtgat cgtctgcatg accaggaagg gcaccaccga caggtcgctc gcggccggct
      481 ggagctcgtc cctgatcttg gccagcacgg cccggccctc ctcggcgtca ccagaccact
      541 cgaccagcga catgatgccg tgccggccga ccagatccgg cggcatccac ggcacctcgc
      601 tggcgcgccg caggaagctg gtccagagca gctcgtcccg ggccgcggga tccgccatct
      661 cgcgcctgcc ttgcccgatc acccggcgcg cctggtccag cgggtggaag gtggccagga
      721 tgaccagctc ggacagctcg tgcagggcga actcgaactc ggtgaccaca ccgaagttgc
      781 cgcccccgcc gcgcagcgcc cagaacaggt cggggtgctg gcccgccgac gcccgcagga
      841 tcctgccgtc cgcggtgacc acctccgcgg acagcaggct gtcgacggtc aggccgaagc
      901 ggcgcatcaa ccggccgacg ccgccgccga gggtgaggcc gcccacgccg gtgtggctga
      961 ccacgccggc cggcaccacg aggccgtgcc gctgggtggc gcggtcgacg tcgccgagca
     1021 gcgccccgcc gccgacggtg gcccgccgcg ttaccgggtc cacggtgacc cggctgagct
     1081 cgcgcaggtc gatcaccatg ccgccgtcgc aggtgctgtg cccgggcatc gagtgcccgc
     1141 cgccgcgaac cgcgatcggc aggccctcgg cgcgggcgaa accgatggcg gcggccacgt
     1201 ccggcgcgga ccgcgggcgg gcgatcagcg cgggcctgcg gtcgtgcatg gcgttccaga
     1261 ccgcccgggc ggcgtcgtag ccgggctggt ccgggcggat cacggtcccg ccgaagccgg
     1321 ggatcgccga tccgggtacc gaacgagcgg ggctcacgcg gctccatccg ccggggagcg
     1381 gtcgccgggc acgcgcagcg cgcacagccg agcgtgcatg ccggcgatga cgtcgttgtg
     1441 ctggtgcacc tcgaccgggt gcgggaacag cgggccgccc tcgtgccagg cgaccgagac
     1501 cgggccggtc ctggcggtgt tgatcaggcg gcaccccagc cgggccagcc gggcgctctc
     1561 ctcggccagg tcgggcacca cccaggagac gtggctgatc gcgccgggcg cgatgcggta
     1621 cgcggccgcc aggcggtcgt cagccgcgtg gacctggccg agctcgacca cgatcggccc
     1681 ccatgcggcg aacgcggccg agtggtcgaa cacgacgtcg gcgcgggcct ggccgccgtc
     1741 ggtgagcacg aactcctcga acggcacgtg ctcgatgacg tcgaacgggc cgacatcgag
     1801 gtcccggtgc cactgcttca tcgcggcgtc caggtcgtcg acccagtagc cgacgtgatg
     1861 caggggatac atcagcggcc ctcgagcgcg ccgccgccga actgcttgtc gtgttcggag
     1921 gtcatccgca tgaacgggta gacgtagtcc cgcatcccct gctcgtaggc cccgatggcc
     1981 gggacgaggg ccgcctcgcc gcgggccgcg gcggagagct ggccgagcag gtgcgcggcg
     2041 tcgcgcagcg ccaggttggc gcccaggccg agcgtgggca gcatggcgtg ggcggcgtcg
     2101 cccaccagcg tgacccggga cggctcccac ggcggggccg gctcgaggaa gccgaacggg
     2161 atcacgaaga cggaggcgag atcgacgccc gcgaccaggg cgcgcgcggc ggggtgccag
     2221 ccctcgatcg cggccagcat ggcgtcgcgg agcatggccg gcgtgccgtc cgtccactgc
     2281 gccgcgggcg ggatcaccgt cccgggcggc acgctgcagc tgatcatgac gtagtcctcg
     2341 accggctcca gcgtgacgtc gggcgcgatc gcggccgccg cgtcttgcgg gcgctgccgg
     2401 ggccggtaga ccgcgatcag cagccgggag cccttgcggt ccgcagcgat gatcacgccc
     2461 tcgaacaggt tcggcggcag ctgcgcggcc agctcggggg tcagcggcgt gcggccgaag
     2521 acgcccagcc cgcggacgcc ggtatcgatg accgtggtgc ccgggagctt ctgcccgcgg
     2581 accgcggagc ggatgccgtc cgcgccgacc aggacgtcgc cctgtgcagt gttgccgttg
     2641 gccagggtga ccgtgacttg gcctggtgtc tcttcgtagc cggtgacggg ctggccgggg
     2701 tggaacgcgt caccgagccg ggcgcgcagg atggcgcgca gggtccgccg gtgcaccccg
     2761 gtgtgcgggc gttcgccctc gttcggcggc cccaggtgcg gctgcgagct cagctcgttg
     2821 agctggtcgt ccaggacgac cgactcgcgc cggtcgtagg tccggcgcga ggtctgcgcg
     2881 tacagctcga acaggtcggc cgacaggcag cgccggagcg cctcgccgcc gtcggcgttc
     2941 atgtgcaggt agtagccgat cttgcgggtg tcgtcggcgt cgcgctcgaa gacctcgcag
     3001 gtgtgcccgt ccttgatcag gccctgggcg agggccaggc cggacaggcc gccgccggcg
     3061 atgaggacgt gcagcggttt cgcggtgctc atgaggtcaa tccgcggagt acaggtcggt
     3121 cgagtacggc ggggtgctgt gcgcggcggc ccagggcacg acgtggttgc ggagcacgcc
     3181 gacgccctcg acctcggcct cgatgctgtc cccgggctgc aggtagaact cgaacgggtt
     3241 gggctggacc gcggccacgc ccgagatcgt cccggtggtg aggatgtctc cggccgagta
     3301 gccctgcgcc gagtggtacg cgaccaggtg cgggatggag atgagcatcc gggccgtgtt
     3361 gccgcgctgc cggacgccgc cgttgacccg cagttccatg gccagcgcct gggcgtcagg
     3421 cacctcgtcg gcggtcacga tccacggtcc gatcgggcag aaggtgtcga tcgccttgga
     3481 gaaggagaac acgccggact gcatctcccg gcgctggatg tcgcgggccg tgatgtcgtt
     3541 gaagaccgtg tacccggcga cgtagcccgc ggcctcctgc gggccgaaga acttgccggc
     3601 cttgccgatg atgatggcga gctcgagctc gtagtcgagc tctttggtca gcccctccgg
     3661 gtagacgatg gcgtcgtccg ggccgatgat cgcgtcgacg ttctggaaga acacgatccc
     3721 cttgtgcacc gggtgcgacc agtcgacggc ctgcagttcc tcgtggtggt cggcgaagtt
     3781 gccggcggtg tggaagaact tcttcggcac gatcggcgcc cgcagcctga cctgcgccag
     3841 cgggagacgc tcccccgtct cgccggcctg gccgccgcgc tcgaaccatt cccgcgtcga
     3901 ggcgcagtcg agctcgacga cgaccgcgtc gtcgccctca ccctccaggc gacccacccg
     3961 accggcgtcg aaggtgacga gcttcatggc ggtttgactc gctctcgttc tacgaaagta
     4021 actttcgtcc atcttcgtgc gcgagcggcg gcggtgtcaa gccggggcgg cgggtccctc
     4081 gggggcagtg acctcggggg gcagtgtcca ccggggcggg gacgcggaac acgcggagct
     4141 ggagcgcgag ggttgcgtag tagccgacca gggtgagcag ctcgaacagc ccggccgggc
     4201 cgaggtgacc gaccgcctcg ccgtactcgg cgtcgtccag gtcgccgcgc gtcacgagcg
     4261 cggcggtcgt gcgcgcgacc acggactcgc gcccggccag gtcggcgtga cggccctcgc
     4321 gcagggcggc cagctcggcg tcgcccaggc cggccgcgcg gccgaccgcc tcgtgggcgt
     4381 gccactcgaa gtcggaccgc cagtgcgcgg cgacgacgag gatcgcgatc tcccggcacc
     4441 ggtcgtcgag cccggtctcg tagcgcaccg aagcgccgag cgcctgcagg gccgccccaa
     4501 gccgcggctg cagcaggaag gcgttgaacg ggccctccag ccgcccgtcg gcatcggcca
     4561 gccggaacag ctgcggcccc tgggcccggc ggcctccggc gatggcgtcg tagagcgacc
     4621 gctgctcggc gtcgagcgcc gacggctcga actttccgat gcgcgccccg gacgtcaaag
     4681 cgagccggcg gtggtgtcgg acagcagcgc ggagatctcc gcgcaggtgg ccagcacggc
     4741 gggcctgagc tcgcggatga tccgctgggt ggaccagtcc tgcgcctgga cggccaggtt
     4801 gacgccggcc agcacgcggc cgtccggcgc ggtgatcggg gcggcgaccg accggagccc
     4861 gtaggccagt tcctcgtcct gcatggccca gccctggtcg cggatggtgc gcagctcgtc
     4921 gcgcagttcg gccagcgaga ccttggcgtt gggccccgag ttggcggcga acgaggcgtc
     4981 ggtgatccgg gactgaaggt cggcctcgtc gaggtgggcg agcagcagct tgccgatcga
     5041 ggtgtgcacg gcggggagcg tggagcccac ctggatgttc gcggtcacca ggtcggaatt
     5101 gcgcaggcgg atgaggtaga ggacgcggtc gccgctgagc acggccagat tcaccgtctc
     5161 ccccgtccgc tcgccgagct gggtgagctt cggcgtcgcg atggccacca ggtcgaggct
     5221 gcgcagcgcg gccgtgccga gcgtcagcgt gcgcaccccg ggccggtagt cgccgttggg
     5281 caggtggtcc aggtacccct cggacgccag cgtcatgacc acccggtaca cggtcggcat
     5341 cggcaggccc gcggcggcgg cgaggtcgct gacccgccag gacgggcgct gctcgtcgaa
     5401 cagcgacagg atgcgcaggc ccttggcgag cgcctccacg cggtactccg ggcgttccgg
     5461 gaccgcgggg accgcgggga ccccggggga cgcgggggac gcgggggacg cgggggcggg
     5521 cctgagcgcc gcggatgagg agctggcggg gcggcgaggc atggtggcct ttcctcctcg
     5581 gtgatgaggg atccgacctc agcctacggt gacgtcggcg gacgcccgcg gtggccccgg
     5641 cggaccctgg acagccgcgc gacgggtcgg ctacggtatt cagaaagcga ctttcataat
     5701 acgagaactc ttacgctaag gatagccccc atgcaggact ggctcacgct cgaccgacgg
     5761 ccgaccctgg tcttcggagc cggcgggctc ggcggggcca gcgcgctgag cctggccagc
     5821 cagggcgcgc gcgtcgcgct cgtcgacgcg agccaggaga acctggacac ggtggtgcgc
     5881 gccgccaagg aggccggccg cgaggtcaag ccgctggtcg ccgacctgcg gacggcggag
     5941 gcctgccggg cggccgtcgg cgaggccgtc gccctggtcg ggacgcccca ggtcttcctg
     6001 cacgccgtcg gccgcaacgt acgcaagccg atcctggagc tggcggacga cgactggcag
     6061 gagaccctca gcctgaacct gtcgtcggcg tactggctcg gccaggccgt cgggcggctc
     6121 atggtcgagc ggcggtacgg ccgcatggtc ttcgtgtcgt cggtctccgg cctgctcgcc
     6181 cacccgtacc acgggcccta cgcggcgacc aagggcggga tgaaccagat gctccgggtc
     6241 atggcgcgcg agtgggcccg gcacggggtc acggtcaacg ccgtcgcgcc cggctacatc
     6301 gagaccgacc tgaccaagga ct
//
LOCUS       DASPYY010000114         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1704521, whole genome shotgun sequence.
ACCESSION   DASPYY010000114 DASPYY010000000
VERSION     DASPYY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704521"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VEP73_01705"
     CDS             <1..393
                     /locus_tag="VEP73_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25188.1"
                     /translation="DPDPRVPDPGSQALDPDPQVPDPQVPDLDLDELAVRTEGLTAAE
                     LLDLCDRLAEAAVEARDPSRPVASRPVASRPITSRPITMADARRLLAELRPGAPAWFA
                     AARDHLRFSGDSDTYGELAAHLRATRAW"
     gene            complement(416..943)
                     /locus_tag="VEP73_01710"
     CDS             complement(416..943)
                     /locus_tag="VEP73_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYT25189.1"
                     /translation="MPPSRAGIVFPGTWTPTSRNGPSRKGRHPCQAHRRQAHRRTACR
                     ARRAQRTRVASGSPYEPEIGFSRALRIGDRVLVSGTAPIWPDGSCDPDPERQARRCLE
                     IILDALASPEIGGRAEHVVRTRMFLVSAADWEAVGRAHGAVFGAIRPAATMVVVAGLL
                     DPSWRVEIEAEAVLT"
     gene            1086..2477
                     /locus_tag="VEP73_01715"
     CDS             1086..2477
                     /locus_tag="VEP73_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYT25190.1"
                     /translation="MLDDAVVRQVLAAALATGGEWAEIYAEDRLSHSIRLDDRRVEEL
                     VTGRDRGAGVRVVRGTSAAYAYTNRLDADALAEAARIASAALRENRRVEVRDLRRREP
                     GVTHPVRVDPSGVERATLAELVRRADDAARGYDPAVAQVTAGYGDVRQHVFIANSEGF
                     TTSEERVRTRFTVQVVAVRDGVVQTGFDGPGRSKGFELLDEFPPEEVARRAAERAVNM
                     LASRPAPAGEMPVVIAAGNGGVLFHESSGHGMEADLVAKDASVYTGKQGKQIGVPTFN
                     GVDDATVEGGYGSFVFDDEGTPAQRTVLFVGGVCTDYLTDLIRARQLGQPASGNGRRQ
                     AYTDVPIPRMTNTYILGGTDDPEEIIGQTPRGLYAKSLGGGQVNPVTGEFVFGVVEGY
                     LIENGQATTPVRGANLVGDGPSVIAAIDALGDDFAVKEGVCGKEGQGVPVGNGAPTLR
                     IARMTVGGTGGTA"
BASE COUNT          353 a          969 c          982 g          304 t
ORIGIN      
        1 gatcccgacc cccgggtccc ggaccccggc tcccaggccc tggaccccga tccccaagtc
       61 ccggatcccc aggtcccgga cctggatctt gacgagctgg ccgtccggac cgagggcctg
      121 acagccgccg agctgctcga cctgtgcgac cgcctggccg aggctgcggt ggaggctcgc
      181 gacccgtccc ggccggtcgc gtcccggccg gtcgcgtccc ggccgatcac ctcccggccg
      241 atcaccatgg ccgacgcgcg gcgcctcctg gccgagctgc ggccgggcgc gccggcctgg
      301 ttcgcggccg cccgcgacca tctcaggttc tcaggcgaca gcgacaccta cggcgagctg
      361 gccgcccacc tgcgcgcgac ccgggcctgg tgaccacctg agcaggggct cccagctagg
      421 tgagcaccgc ctcggcctcg atctcgaccc gccacgaagg gtcgagcagg ccggccacca
      481 cgaccatggt ggcggcgggc cggatggcgc cgaacaccgc gccgtgcgcc cgcccgaccg
      541 cctcccagtc cgcggccgag acgagaaaca tccgggtgcg gacgacgtgc tcggcccggc
      601 cgccgatctc cggcgaggcc agggcgtcga ggatgatctc gaggcagcgg cgggcctgcc
      661 gctccgggtc agggtcgcac gagccgtctg gccagatggg cgcggtcccc gagacgagga
      721 cgcggtcgcc gatcctcagc gcccggctga agccgatctc gggctcgtag ggcgacccgg
      781 aggcgacccg ggtccgttgc gcccggcggg ctcggcaggc ggtgcgccgg tgtgcttgac
      841 gccggtgtgc ttgacatgga tggcgtcctt tgcgcgaggg tccgttgcgc gaggttgggg
      901 tccacgtccc ggggaacacg ataccggcgc gcgagggggg caccacctgc ctggcgtggc
      961 ggcgccacct ctgcggcgcc acctctacaa tggcgcgcgc ccacggccgg cgggcggcgc
     1021 gaccgcgcca cccgcgccac ccgcgccacc gacccggccg cgccttgccc gaaggagtac
     1081 agcccgtgct ggatgacgcc gtcgtccgtc aggtcctggc cgccgcgctc gcgacgggcg
     1141 gcgagtgggc cgagatctac gccgaggacc gcctctccca ctcgatccgg ctcgacgacc
     1201 gcagggtcga ggagctggtc accggccgcg accggggcgc cggggtccgc gtggtgcgcg
     1261 gcacctcggc cgcctacgcc tacaccaacc ggctggacgc cgacgcgctg gccgaggcgg
     1321 cccggatcgc gtcggccgcg ctgcgcgaga accgccgggt cgaggtccgc gacctgcgcc
     1381 gccgcgagcc gggcgtgacc cacccggtca gggtcgaccc gtccggggtg gagcgggcca
     1441 cgctggccga gctggtccgc cgggccgacg acgccgcgcg cggctacgac ccggcggtcg
     1501 cccaggtcac cgccgggtac ggcgacgtgc gccagcacgt gttcatcgcc aactcggaag
     1561 ggttcacgac ctccgaggag cgggtccgga cccgcttcac cgtgcaggtc gtcgccgtcc
     1621 gcgacggcgt cgtccagacc ggcttcgacg ggccgggccg ctcgaagggg ttcgagctgc
     1681 tcgacgagtt cccgcccgag gaggtggcgc gcagggcggc cgagcgggcg gtcaacatgc
     1741 tcgcgagccg gccggcgccg gccggggaga tgccggtggt gatcgcggcg ggcaacggcg
     1801 gggtgctgtt ccacgagtcg agcggccacg gcatggaggc cgacctggtc gccaaggacg
     1861 cctccgtcta caccggcaag cagggcaagc agatcggggt cccgaccttc aacggcgtcg
     1921 acgacgccac cgtcgagggc ggctacggct cgttcgtgtt cgacgacgag ggcacccccg
     1981 cccagcgcac ggtgctgttc gtgggcggcg tctgcaccga ctacctgacc gacctgatcc
     2041 gggccaggca gctcggtcag ccggcctcgg gcaacggccg ccgccaggcg tacaccgacg
     2101 tcccgatccc gcggatgacc aacacctaca tcctcggcgg gaccgacgac ccggaggaga
     2161 tcatcgggca gacgccgcgg ggcctgtacg ccaagtcgct cggcggcggc caggtcaacc
     2221 ccgtcaccgg ggagttcgtg ttcggtgtcg tcgagggcta cctgatcgag aacggccagg
     2281 ccacgactcc ggtacggggc gcgaacctgg tcggcgacgg ccccagcgtc atcgccgcga
     2341 tcgacgcgct gggcgacgac ttcgcggtca aggaaggcgt ctgcggcaag gaggggcagg
     2401 gcgtgccggt cggcaacggc gccccgacgc tccgcatcgc gcgcatgacg gtcggcggga
     2461 cgggagggac ggcatgacgg tcgagctgga gcgcatcgtc gggcggctgg ccgcggaggc
     2521 cggcggcgac gagggggtcg aggcgtacgc cgagcaggtg accgagacga acgtcaccgc
     2581 ctaccagggc gaggtcgagc ggctcacc
//
LOCUS       DASPYY010000115         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1704875, whole genome shotgun sequence.
ACCESSION   DASPYY010000115 DASPYY010000000
VERSION     DASPYY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704875"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VEP73_01720"
     CDS             <1..1232
                     /locus_tag="VEP73_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYT25191.1"
                     /translation="LARNLAELRATVVQTEQRRREGGSVAKLVHRQVALEREIRDYCR
                     HQRGEPTWQEASPPSFDRELGDELGQAALVEFVHLDETLHAVTVVHGRPRLRQLGPTA
                     QVRALIERLAFALRRLTRHHADEASRAAAAVALRHAAARLDAMLLRPLADRIGERPLL
                     VVPTGPLQSLPWSILPSLAGRPLSVSPSAGLWRMASRRPPRPGGHVVVADGGLPGARM
                     EAKTVAAIYHTTALLDPLPTVGALTDALHRAALLHLAVHGRLRADNPLFSSVDLADGP
                     LTVYDLERLERVPQTVVLAACESGRTVVLAGDELLGLSATFLSHGTRQLVASVVTIPD
                     LETAPLMIAFHRLLAAGVPAAEALARAQREVPWDDWTAVAAAAGFVCIGDGFGLPGPP
                     PPQAGRDGRPTRGRPGG"
     gene            complement(1245..>1643)
                     /locus_tag="VEP73_01725"
     CDS             complement(1245..>1643)
                     /locus_tag="VEP73_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25192.1"
                     /translation="GEPPRVQPSKRRPAAGQLAREGIVPPPALGKASGPTGRRRTTMP
                     LIEVARAATRAEALEGLERWKARHPAVWSYLAPADVLEDSMRGRSSTWTRIRLNLRNV
                     PDAERPAQEALEVDYDPWERHPGPGGPGRD"
BASE COUNT          189 a          640 c          598 g          216 t
ORIGIN      
        1 tcctcgcccg caacctggcc gagctacggg cgacggtcgt ccagacggag cagcgtcgcc
       61 gggagggagg cagcgtcgcc aagctggtcc accgtcaggt ggcgctcgag cgcgaaatcc
      121 gcgactactg ccgtcaccaa cgcggcgagc cgacctggca ggaggcgagc ccaccgtcgt
      181 tcgaccggga gctgggcgac gagctcggtc aagccgcgtt ggtcgagttc gtccacctcg
      241 acgagaccct ccacgccgtc accgtggtgc acggaaggcc gcgcctgcgc cagctggggc
      301 cgacggcgca ggtacgggca ctgatcgaac gtctcgcgtt cgcgctgcgc cgcctcaccc
      361 ggcaccacgc cgacgaggcc agccgcgccg ccgccgcggt cgcgctgcgg cacgcggcgg
      421 cgcggctgga cgcgatgctc ctgcggccgc tcgccgaccg gatcggcgag cggccgctcc
      481 tcgtcgtccc gaccgggccg ctgcagtccc tgccctggtc gatcctgccg tcgttggccg
      541 gccggccact cagcgtgtcg ccgtcggcag ggctgtggcg catggccagc cgacgtccgc
      601 ccaggcccgg cgggcacgtc gtcgtggccg acggggggct gccgggcgca cggatggagg
      661 cgaagacggt cgccgcgatc taccacacga ccgccctgct cgacccgctg ccgaccgtcg
      721 gggcgctcac cgacgcgttg caccgggcgg ccctgctgca ccttgcggtg cacgggcgtc
      781 tgcgggccga caacccgctg ttctcctcgg tcgacctcgc cgacggcccg ctgaccgtgt
      841 acgacctcga gcgcctggag cgagtgcccc agacggtcgt cctcgcggcc tgcgagagcg
      901 gtcggacggt cgtgctggca ggagacgagc tgctcgggct cagcgccacc ttcctctccc
      961 acggcacccg gcagctcgtc gcgtccgtcg tgacgatccc ggatctcgag acggctccgc
     1021 tcatgatcgc cttccaccgc ctcctcgcgg ccggggtccc ggccgccgag gcactggccc
     1081 gcgcgcaacg ggaggtcccg tgggacgact ggacggcggt ggccgcggcg gcggggttcg
     1141 tctgcatcgg ggacgggttc gggctgcccg gccctccccc gccgcaagca ggtcgagacg
     1201 ggcgaccgac cagggggcga cccggtgggt gaagcgcaca ctggctagtc ccgccccggg
     1261 ccgcccgggc ccgggtgccg ctcccagggg tcgtagtcga cctccagcgc ctcctgggcc
     1321 gggcgttccg cgtcgggcac gttgcgcagg ttcagccgga tccgcgtcca ggtgctgctg
     1381 cgtcctcgca tggagtcctc cagcacgtcc gcgggagcca ggtaggacca gacggctggg
     1441 tgccgggcct tccagcgctc cagtccctcc aacgcctcgg ccctggtggc cgcccgggcg
     1501 acctcgatca gcggcatggt cgtccggcgg cgcccagtcg gcccgctcgc cttccccagc
     1561 gccgggggcg ggacgatgcc ctcgcgcgcc agctgccccg cggccggccg ccgcttcgag
     1621 ggctgcaccc ggggcggctc acc
//
LOCUS       DASPYY010000116         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_19545, whole genome shotgun sequence.
ACCESSION   DASPYY010000116 DASPYY010000000
VERSION     DASPYY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19545"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VEP73_01730"
     CDS             <1..1349
                     /locus_tag="VEP73_01730"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT25193.1"
                     /translation="IRLRTGVDSEGRFVAHDARVVFDGGAYASAKPLPHLVLAGGSAT
                     LAAYRVPHARVEVSSVYTNTAPCGHMRSPGEVQALFAGESHVDMIAAELGIDPLELRL
                     RNALRDGDQGLGGEGFRQARAVPVLEALREAGGWERRPAGQGRGRGIALGVRHVGGGK
                     TSVHLRAGADGRVEVLTGLPDQGGGALTVIHRVAAATLEVAPGRIVVTRGSTGSAPAD
                     PGVGGSRVTHLASRATQSGALELKRRLEAAATEVFGNGRPLQLADDRFHGGGEGPIPF
                     ERVAERLAGDGIEVEGAYEAAREPGDWDFVACQVEVEVDLDTGQPTVREALLVVDVGT
                     IINPVAHRGQLVGGFVFGLGGALMEELRVEDGIVTSGTLGEVKLPTSMDCPPLRIVEL
                     PTAVGPGAFGAKMAGELTNSVVAPAVANAVAAATGARVTSLPITAERIYWALSAPG"
     gene            1394..>1694
                     /locus_tag="VEP73_01735"
     CDS             1394..>1694
                     /locus_tag="VEP73_01735"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HYT25194.1"
                     /translation="MITGGTIDSVGTDRLDLGWYLEAGKRLGDGELVARVPELDQIAA
                     VTEIPFRRLPSQSITTADWLDMIKTIQDLFAGDRVDGVVITHGTNTLEETAYFLHL"
BASE COUNT          221 a          612 c          652 g          209 t
ORIGIN      
        1 ccatccgcct gcgcaccggc gtggactcgg agggccgctt cgtcgcccac gacgcccggg
       61 tggtcttcga cggcggcgcc tacgccagcg ccaagccgct gccgcacctg gtgctggccg
      121 gcgggtcggc gaccctggcg gcctaccggg tgccgcacgc tcgcgtcgag gtctccagcg
      181 tctacaccaa caccgccccg tgcggccaca tgcgctcgcc cggcgaggtg caggcgctgt
      241 tcgccggcga gtcgcacgtc gacatgatcg ccgccgagct gggcatcgac ccgctggagc
      301 tgcggctgcg caacgcgctg cgcgacggcg accagggcct cggcggcgag gggttccgcc
      361 aggccagggc ggtgccggtg ctggaggcgc tgcgcgaggc cggcggctgg gagcggcgcc
      421 ccgccggcca gggccgcggc cgcgggatcg cgctgggcgt gcggcatgtc ggcggtggca
      481 agacctcggt gcacctgcgc gccggcgccg acggccgggt cgaggtgctc accggcctgc
      541 ccgaccaggg cggcggcgca ctgaccgtca tccaccgggt cgccgccgcg accctggagg
      601 tcgcgcccgg gcggatcgtg gtgacgcgcg gcagcaccgg cagcgcgccg gccgatcccg
      661 gggtcggcgg cagccgcgtc acccacctgg caagccgcgc cacccagagc ggggcgctgg
      721 agctgaagcg ccggctggag gcggccgcca ccgaggtgtt cggcaacggc cggccgctgc
      781 agctggcgga cgaccggttc cacggcggcg gcgaggggcc gatccccttc gagcgggttg
      841 ccgagcggct cgccggcgac gggatcgagg tggagggcgc ctacgaggcg gcgcgcgagc
      901 ctggcgactg ggacttcgtc gcctgccagg tggaggtcga ggtcgacctg gacacgggac
      961 agccgaccgt gcgggaggcg ctgctggtgg tcgacgtcgg cacgatcatc aacccggtgg
     1021 cgcaccgcgg gcagctcgtg ggaggcttcg tgttcgggct cggcggcgcg ctgatggagg
     1081 agctgcgggt cgaggacggc atcgtcacca gcggcacgct cggcgaggtc aagctgccga
     1141 ccagcatgga ctgcccgccg ttgcggatcg tggagctgcc gacggcggtc gggccagggg
     1201 cattcggcgc caagatggcg ggcgagctca ccaactcggt ggtcgcgccg gcggtcgcca
     1261 acgccgtcgc ggcggcgacc ggcgcccggg tcacctccct gccgatcacc gccgagcgga
     1321 tctactgggc gctcagcgcc ccaggctgat cgcgtactct agcatggcgg aggctcgcca
     1381 gcgcgtggcg ctgatgatca ccggagggac catcgactcg gtcggcaccg accgtctcga
     1441 ccttggctgg tacctggagg ccggcaagcg gctcggcgac ggcgagctgg tcgcccgagt
     1501 gccggagctc gaccagatcg ccgcggtcac cgagatcccc ttccggcgcc tgcccagcca
     1561 gtcgatcacc accgccgact ggctcgacat gatcaagacc atccaggacc tgttcgccgg
     1621 cgaccgcgtg gacggggtgg tgatcaccca cgggaccaac accctcgagg agaccgccta
     1681 cttcctgcac ctca
//
LOCUS       DASPYY010000117         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1564996, whole genome shotgun sequence.
ACCESSION   DASPYY010000117 DASPYY010000000
VERSION     DASPYY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564996"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VEP73_01740"
     CDS             <1..705
                     /locus_tag="VEP73_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYT25195.1"
                     /translation="RRVKELHQRGIRAVKLRFHGDWRRGLEVVEAVRDALGSQVELMV
                     DANQGWRMPGDLTPRWDVPTAAQCAAELERLGVYWLEEPLPTDDLDGYRALRHRTSMR
                     LAAGEMVRHLHEARDLVLRGGVDVVQPDAVLAGGIAGCRRVAALAALCGRTFSPHTWS
                     DGLGLVANLHLALAVSTCPYVEVPLDPPAWSAERRDWLLPEPVDVAADGTIQPPPGAG
                     LGVTPDLEALEPYRVG"
     gene            736..>1839
                     /locus_tag="VEP73_01745"
     CDS             736..>1839
                     /locus_tag="VEP73_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYT25196.1"
                     /translation="MAHGTRTVRAAVLHQARTPLVVEEVRLEPPRAGEVLVRVAAAGV
                     CHSDLHLAEGHLGDGRWPTVLGHEGAGVVEEVGAGVGHVAPGDPVAFCFVPSCGTCLE
                     CRAGRRNLCRTAADRSWAGTLLDGTSRLSLSDGRPLRHFNFVSCFADRCVVPAASAVP
                     VPPVLPLWQAAVIGCGVVTGVGAVRHAARVEPGESVAVVGCGGVGLQVVAGARLAGAH
                     PVVAVDRDPAKLERALAAGATHALDALRGDPVAATLELTGIGVDHAFEVVGRPETIRL
                     AWDLLRPGATAVVVGIVPRGVDVSVPGIELLAEKGLKGSYYGSGDPPADVAELARLAA
                     DGLLDLATGVSDLTDLDGLEAAFERLRRGGGAAR"
BASE COUNT          184 a          670 c          751 g          234 t
ORIGIN      
        1 cgcagggtca aggagctgca ccagcggggt atccgcgcgg tgaagctgcg cttccacggc
       61 gactggcggc gcggcctcga ggtcgtggaa gcggtgcgcg acgcgctcgg ctcccaggtg
      121 gagctgatgg tcgacgccaa ccaggggtgg cgcatgcccg gcgacctcac gccgaggtgg
      181 gacgtgccga cggccgcgca gtgcgcggcc gagctcgagc ggctgggcgt ctactggctc
      241 gaggagccgc tgccaaccga cgacctcgac ggctaccggg cgctgcggca ccgcacctcg
      301 atgcggctcg cggccggcga gatggtccgc cacctccacg aggcgcgcga cctggtgctg
      361 cgcggcgggg tcgacgtcgt ccagcccgac gccgtgctcg ccggcgggat cgccggctgc
      421 cggcgggtcg ccgccctggc ggccctgtgc ggcaggacgt tctcgccgca cacctggagc
      481 gacggcctcg ggctggtggc caacctccac ctcgcgctcg ccgtctcgac ctgcccgtac
      541 gtcgaggtgc cgctggaccc gccggcgtgg tccgcggagc gccgcgactg gctgctgccg
      601 gagccggtcg acgtcgccgc cgacggcacc atccagcccc cgcccggtgc cggcctcggc
      661 gtcacgccag acctcgaggc cctcgagccc taccgggtcg gttgacgtcg ggtggcgcac
      721 gagacccgac gtccggtggc gcacgggacc cgcacggtgc gggccgcggt gctgcaccag
      781 gcccggacgc cgctggtggt cgaggaggtg cgactggagc cgccgcgggc cggcgaggtg
      841 ctcgtgcgcg tcgcggcggc cggggtctgc cactccgacc tgcacctcgc cgaggggcat
      901 ctcggggacg ggcgctggcc gaccgtgctc ggccacgagg gcgccggcgt ggtcgaggag
      961 gtcggcgcgg gcgtcgggca cgtggcgccc ggcgacccgg tggcgttctg cttcgtgccg
     1021 agctgcggca cctgcctgga gtgccgggcg ggacggcgga acctctgccg tacggcggcc
     1081 gaccggtcct gggcggggac cctgctcgac gggacctcgc gcctgtcgct cagcgacggc
     1141 cggccgctca ggcacttcaa cttcgtctcg tgcttcgccg accggtgcgt ggtccccgcg
     1201 gcttcggccg tcccggtccc gccggtcctg ccgctgtggc aggcggccgt gatcggctgc
     1261 ggggtggtga ccggagtcgg cgcggtgcgc cacgccgcgc gcgtggagcc gggcgagagc
     1321 gtggcggtgg tgggctgcgg gggcgtcggc ctgcaggtcg tcgcgggggc gcgccttgcg
     1381 ggggcccacc ccgtggtcgc ggtcgaccgc gacccggcca agctcgagcg ggcgctcgcg
     1441 gccggggcga cccacgcgct ggacgcgctg cgcggcgacc cggtcgcggc gacgctcgag
     1501 ctcaccggga tcggcgtcga ccacgcgttc gaggtggtgg gacggcccga gacgatccgg
     1561 ctggcctggg acctgctgcg gcccggcgcg accgcggtgg tggtggggat cgtgccgagg
     1621 ggggtggacg tgtcggtccc cgggatcgag ctgctcgccg agaagggcct caagggctcc
     1681 tactacgggt ccggggatcc gccggcggac gtcgccgagc tcgcgcgcct ggcggccgac
     1741 gggctgctcg acctcgccac cggggtctcc gacctcaccg acctcgacgg cctcgaagcg
     1801 gcgttcgagc gcctgcgccg gggcggcggg gcggcgcgc
//
LOCUS       DASPYY010000118         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_19816, whole genome shotgun sequence.
ACCESSION   DASPYY010000118 DASPYY010000000
VERSION     DASPYY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19816"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..483
                     /locus_tag="VEP73_01750"
     CDS             220..483
                     /locus_tag="VEP73_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25197.1"
                     /translation="MVVPISSARARVRARFEGEVRRIRIHSLAAVPTLEIQLDDGTGR
                     ISALFMGRRGISGIECGRRLAVEGTPVAVERGLTLYNPAYDLR"
     gene            complement(509..1252)
                     /locus_tag="VEP73_01755"
     CDS             complement(509..1252)
                     /locus_tag="VEP73_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYT25198.1"
                     /translation="MKVAIAGAGNVGQFIAADLLAAGHQVTLLEKDPAVIEAARGSLD
                     QAEWFPFDACEVLSLVMAGIAHADVVVAATGDDEDNLVISLLAKQEFAVPRVVARVNN
                     PKNHGLFNEAWGVDVAVSTPHILRSLVEEAVSVGSMVRLMQFEQGKVSLHEVTLAEGS
                     PSIGQTLRDLALPVDAAVVAVVREGHVVVPKEDTVFAVGDEVLVVVSEGTEDYVRKML
                     VGGQPEPEGGMAIAGTAAQNPQRLADPRG"
     gene            complement(1249..1965)
                     /locus_tag="VEP73_01760"
     CDS             complement(1249..1965)
                     /locus_tag="VEP73_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYT25199.1"
                     /translation="MHVVIMGCGRVGSELAARLEARGHTVAIIDKNQNAFRSLRYGCD
                     CQKVAGYGFDEAVLVQAGIERAGAFAAVSNGDNSNIVAARLVREKFEVPIVVARIYDP
                     RRAEIYQRLGIPTVATVKWTTDQVMRFLIPEQLASDWKDPTEGVSLVTLVLPPDWAGW
                     RIDELESDGHRRVVAVTRNGQAHIVAPSLILQEGDQVHLAVDNSGLDELRPMVLAARK
                     SQQEELDEQTRTERSEEVRS"
BASE COUNT          299 a          825 c          792 g          307 t
ORIGIN      
        1 actatctcga gccctaccgg ccggccaagg acggcatcga cgtcgaggcg ctgagcgccg
       61 cgctccgggc agcggtggcg gcctgccggg ccgcgctggt ggacggccgg ccaccggcga
      121 tcgagatggt gaagctccgg ctcggccgcg cggatgccca gtaggaacga gcggcgcccg
      181 cggcgccccg caccagtccc cccggagctc gaggcgcccg tggtggtccc gatctcctcg
      241 gcccgggcgc gcgtccgggc ccgcttcgag ggcgaggtcc gccgcatccg catccactcg
      301 ctcgccgccg tgcccacgct ggagatccag ctcgacgacg gcacgggccg gatctccgcc
      361 ctgttcatgg ggcgccgcgg catcagcggc atcgagtgcg gccgccgcct ggccgtcgag
      421 ggcaccccgg tggccgtcga gcggggcctg accctctaca accccgccta cgacctgcgg
      481 tgagccccgg tcccgcgtcc cgggccggtc agccgcgcgg gtcggccagg cgctgcgggt
      541 tctgggcggc cgtgccggcg atcgccatgc ccccctcggg ctcgggctgc ccgccgacca
      601 gcatcttgcg cacgtagtcc tcggtcccct ccgagaccac cacgaggacc tcgtcgccga
      661 cggcgaacac ggtgtcctcc ttggggacga ccacgtggcc ctcgcggacc acggcgacca
      721 cggccgcgtc gaccggcagc gcaaggtcgc gcagcgtctg cccgatcgac ggggagccct
      781 cggccagcgt gacctcgtgg agggagacct tgccctgctc gaactgcatg aggcggacca
      841 tgctgccgac gctgaccgcc tcctcgacga gcgagcgcag gatgtgcggc gtcgagacgg
      901 ccacgtcgac accccacgcc tcgttgaaca ggccgtggtt cttggggttg ttcacgcgcg
      961 cgaccaccct gggcacggcg aactcctgct tggcgagcag ggagatgacg aggttgtcct
     1021 cgtcgtcccc tgtggccgcc accaccacgt cggcgtgcgc gatcccggcc atgacgaggg
     1081 acagcacctc gcaggcgtcg aaggggaacc actcggcctg gtcgagggag ccgcgcgcgg
     1141 cctcgatcac ggccgggtcc ttctcgagca gcgtgacctg gtggccggcg gcgaggaggt
     1201 cggcggcgat gaactggccg acgttgccgg ccccggcgat cgcgaccttc acgagcgcac
     1261 ctcctcggag cgctcggtcc tggtctgctc gtccagctcc tcctgctggc tcttgcgggc
     1321 cgccaggacc atgggccgca gctcgtccag cccggagttg tcgacggcca ggtggacctg
     1381 gtcgccctcc tgcaggatca ggctgggcgc cacgatgtgg gcctggccgt tgcgcgtcac
     1441 cgcgaccacg cgccggtggc cgtcggactc gagctcgtcg atccgccagc ctgcccagtc
     1501 gggcgggagg accagggtga cgagcgagac gccctcggtc gggtccttcc agtcggaggc
     1561 cagctgctcc gggatgagga agcgcatcac ctggtcggtg gtccacttga cggtcgcgac
     1621 ggtggggatc ccgagccgct ggtagatctc ggcccggcgc gggtcgtaga tgcgggcgac
     1681 gacgatcggg acctcgaact tctcgcgcac gagccgggcg gccacgatgt tggagttgtc
     1741 gccgttggag accgcggcga acgcgccggc ccgctcgatg ccggcctgca ccaggaccgc
     1801 ctcgtcgaag ccgtagccgg ccaccttctg gcagtcgcac ccgtagcgga gcgaccggaa
     1861 ggcgttctgg ttcttgtcga tgatcgcgac cgtgtgcccg cgggcctcca gccgggcggc
     1921 cagctccgag cccacccgcc cacaccccat gatgacgacg tgcaactcgg ctccctttcc
     1981 gtcggcgcct ggtcgggtcg accgatcatc gtgccatctt agccgcacgg accggaccgg
     2041 aacgccgtgt gcccgccccg ctgaccggcg gctgtcgagg cggcatactg gctggaagac
     2101 accggccacg gcgatgcaag gacggaggcg ccagcgttgg ccacggagcc acagcaacag
     2161 atcgaccggc cgcgggtcga ccagccgcgg atcgaagggg ccccgccgcc tccgcgcgag
     2221 tcc
//
LOCUS       DASPYY010000119         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1986274, whole genome shotgun sequence.
ACCESSION   DASPYY010000119 DASPYY010000000
VERSION     DASPYY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986274"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1516..1592)
                     /locus_tag="VEP73_01765"
     tRNA            complement(1516..1592)
                     /locus_tag="VEP73_01765"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:His,seq:gtg)
     gene            1758..2249
                     /locus_tag="VEP73_01770"
     CDS             1758..2249
                     /locus_tag="VEP73_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25200.1"
                     /translation="MPHEITSARIRLRQLAPDEARALLEGRPDPARPWARGYPTEGTF
                     TAVEAFVRAASAGAEPSPYGVYQLVRLDDEAVIGDIGFFGPPSPTGVVTVGYGLAASA
                     RGKGYATEALRALTAWALAQPGVRRIEADTTHANVPSQRVMERVGMRLVGQDDQLRYY
                     ALP"
     gene            2465..3064
                     /locus_tag="VEP73_01775"
     CDS             2465..3064
                     /locus_tag="VEP73_01775"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HYT25201.1"
                     /translation="MRMVLMGPPGAGKGTQGKRLAAEFGGAHVATGDLFRANAERGTE
                     LGRIAQEYMDRGDLVPDDIVIAMVTERLAEPDARGDFVLDGFPRTVPQAEALDRRLAE
                     LGLPLQAALNFEISKDELLRRLAGRAKQEDRAEDEDAEAIERRLESFVNDTRPVVDYY
                     DRQGKLLHVDAMGQVDEVTKRIIDELASRGLTSRDHGAR"
     gene            3094..3933
                     /locus_tag="VEP73_01780"
     CDS             3094..3933
                     /locus_tag="VEP73_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT25202.1"
                     /translation="MIALVTGASSGIGAATARRLAREPGARLVLVARRRERLEALAAE
                     LGGATVIAADLTDPGAPARIAAEVEREHGRLDLLVNNAGAPGRGSFGRGGWAGVQAIM
                     ALNFDSQVRLTEALLPLLRRSVPSAIVNVASVAGRVARASAGDYSASKFALTGWTEAL
                     HLEERRHGVHVGLVLPGFVATEGFPQRELVNRRLTRWLVSSEDKVAEAIVDAGLRRKA
                     ERYAPRPYALVPVVRALAPPLYRRAVGGGRRFTPGTATNPVQEAGRVPDGHEPPAGPG
                     PAS"
     gene            complement(3938..>4714)
                     /locus_tag="VEP73_01785"
     CDS             complement(3938..>4714)
                     /locus_tag="VEP73_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25203.1"
                     /translation="LLGAQSTLQVGRQLYTFGLSTPDNRLVTGGRPQVWTARDETSRA
                     AGPFTAEWFVLDAYQKTGDTSPRSDLTGFYAARVDFPAAGNWLVAATVDSGGQQGVAR
                     GAVPVAADRVPARIGSKAVAAATLVATDRADLAKVCTRTPPCDLHAISLDRALASGRP
                     TVVCFATPLFCSSRMCGPVVDELLTVAGKVRPPQANLLHVEIYPDQKSPGKPVAAFTA
                     WGFQTEPWTVVVDRKGIIRASFEGPVTGAQIDSALRPLLR"
BASE COUNT          686 a         1729 c         1702 g          597 t
ORIGIN      
        1 cgaacagcca gccggccggg taggcggtct cctcccgccg gtagctgatg aagatccggc
       61 ctggagctgt cggaggggat gcagccatga tctcttcctc ggggacgccc tacccccgtt
      121 ccagccaggc agcatcagac ccccaagcac agagccgctc gcctgagtgg ctcctgtcag
      181 cccggcggca gggagcgcct aacgataacg gacggatcgt aacggtcggg cgcgccgcaa
      241 ggaagggcat gtgttgcgga gcacccggag ctggtgtcgc agagcacgtt cacccgaggg
      301 gtgatcgccg agcaccgcac ccaacgctgg cccaatggtg tggggggctc ccggctcgat
      361 catccagatg agcaccccag gctcatcgag cccgaagcta taaggccctc aacgaggcgc
      421 ttgccgcgct gcggaccgat accttcatcg agccttccca gcggactctc ggaagcttcc
      481 tggtggacga gtggctcccc gccgtccggc cgcccaaggt gcggccgtca acctggctga
      541 gctatcagct caacgtcgag gggcacatcg tcccggcgct cgggcgggtc ccgctccagc
      601 ggcttacgcc gccccagctc accgccttct accggacgct gctggacggc gacggcgcaa
      661 tcgtcgcggc ggtcttgccc cgaagaccgt ccgcaacatt catggtgcct tacataccgc
      721 gctcaaggat gccgttcgct ggggctatgt cgcccgcaac gtcgccgccg cagccgaccg
      781 gcccaatggc atgacgcccg agatgcacgt ctggagtccc gagcagctcc gcaccttcct
      841 ggagcacgtc caagaggacc ggctctatgc cgcctggctg ctgctggcca ccacgggcat
      901 gcggcgcggg gaggttgcgg gtcttcgctg ggcggacatc gacctcgacg ccggacgggt
      961 ctcgccgcgg cgacctcgtg tggtcgtcag gtatgaggtg attgtgtcgg agccgaagac
     1021 ggccaagggc cgccgctcgc tcgcgctcga ccccgccacg atcgcggccc tacgagagca
     1081 ccgcatgagg cagcttgagg atcggcttgc ggtcgggccg cgctggcagg actccggtct
     1141 ggtgttcacc tggccagatg gccggccgct gcacccgagc ggttctcgaa atggttcgag
     1201 cagcacaccc gggcggccgg cctcccgaag attcgactgc acgacgtgcg ccacagctac
     1261 gcgaccgcga cgcttgccgc cgggatcccg gccaaggtcg tgagcgagcg gctcggccac
     1321 gccaacatcg ccatcaccat ggacacatac agccacgtgc tacccggcct tgatgcccag
     1381 gcggccggca cagtggcccg gctcatcctc ggtccgccgc ggtccaacca gcccgctgct
     1441 gtcaaaccgc tgtcatccgg ccgcaaggcc acacacaggc gaagggaggt gaagcgcgaa
     1501 acgcctggtc agaactgggg tgaccgaggg gactcgaacc cccgaccttc gggaccacaa
     1561 cccgacgctc taaccaactg agctacgatc accaggtcgt caagcgaggc gaaggatacc
     1621 ggcccggggg cgcgcccgcc agcggcgaga ggcttgcagg cccggccgcc gcatggacga
     1681 ccggtgccgt ggcccgggcg agccgtcggt gcccggccgc gttgaacggg cggcggccgg
     1741 cgggttaggt tccggccatg ccccacgaga tcacgtccgc gcgcatccgc ctgcggcagc
     1801 tcgcgccgga cgaggcgcgc gccctgctcg agggcaggcc ggacccggcg cggccgtggg
     1861 cgcgcggcta cccgaccgag gggacgttca ccgccgtcga ggcgttcgta cgagccgcca
     1921 gcgccggcgc cgagccgagc ccctacgggg tgtaccagct cgtccggctc gacgacgagg
     1981 cagtgatcgg cgacatcggg ttcttcggcc cgccgagccc gaccggggtg gtgacggtcg
     2041 ggtacggcct cgccgcgagc gcccgcggga agggatacgc gaccgaggcg ctgcgcgcgc
     2101 tgaccgcctg ggcgctcgcc cagcccgggg tgcgccggat cgaggcagac accacccacg
     2161 cgaacgtgcc gtcccagcgg gtcatggagc gggtcggcat gcggctcgtc ggccaggacg
     2221 accagctccg ctactacgcg ctgccatagc cggggcccgg gcgcccgctg gtcgccccgt
     2281 tgccgacccg gccatacccg tggttgcatg cgggcacggc cgacgccgtg gacgccaggc
     2341 cgccggggga gacgacccgg tgcgcggcac accgtccggt ggcatccaca cgtggggaga
     2401 accaggctcg acagggagcc gccccgtcct ccgagacggc gatgctcgca aggggagtgc
     2461 acacatgcga atggtcctga tgggcccgcc gggcgcgggg aagggcaccc agggcaagcg
     2521 gctcgccgcc gagttcggcg gggcccacgt cgccaccggt gacctgttcc gggccaacgc
     2581 cgagcgcggg accgagctcg gccggatcgc ccaggagtac atggaccggg gcgacctggt
     2641 gcccgacgac atcgtgatcg ccatggtgac cgagcggctg gccgagccgg acgcgcgcgg
     2701 cgacttcgtg ctcgacggct tcccccggac ggtcccgcag gccgaagccc tcgaccggcg
     2761 cctggccgag ctcggccttc cgctccaggc cgccctgaac ttcgagatct caaaggacga
     2821 gctgctgcgg cgcctggccg ggcgggcgaa gcaggaggac cgcgccgagg acgaggacgc
     2881 cgaggcgatc gagcgccgcc tcgagtcctt cgtcaacgac accaggccgg tcgtcgacta
     2941 ctacgaccgg cagggcaagt tgctccacgt cgacgcgatg ggccaggtcg acgaggtcac
     3001 caagcgcatc atcgacgagc tggcctcgcg cggcctcacc agccgcgacc acggcgcacg
     3061 ctgaacgggg ttgaagctga actggagggt gagatgatcg cgctggtcac cggggcgtcg
     3121 agcgggatcg gggccgcgac cgcgcgccgc ctcgcccggg agccgggcgc gcgcctggtg
     3181 ctcgtcgccc gcaggcgcga gcgcttggag gcgctcgcgg ccgagctcgg cggtgccacc
     3241 gtgatcgccg ccgacctcac cgaccccggc gcgcccgccc gtatcgccgc cgaggtcgag
     3301 cgcgagcacg gccgcctcga cctcctggtg aacaacgcgg gcgccccggg acgcggcagc
     3361 ttcggccgcg gcggctgggc cggcgtacag gcgatcatgg cgctgaactt cgacagccag
     3421 gtccggctga ccgaggcgct gctgccgctc ctgcgccgct cggtcccgag cgcgatcgtc
     3481 aacgtcgcga gcgtcgccgg ccgggtcgcc cgggcctcgg caggggacta ctcggccagc
     3541 aagttcgccc tgaccggctg gaccgaggcc ctccacctgg aggagcgccg gcacggcgtg
     3601 cacgtcgggc tggtcctgcc cggcttcgtc gccaccgagg gcttccccca gcgggagctc
     3661 gtgaaccgcc ggctcacccg ctggctggtg tcgagcgagg acaaggtggc cgaggcgatc
     3721 gtggacgccg ggctgcggcg caaggcggag cggtacgccc cccgccccta cgcgctcgtc
     3781 ccggtcgtcc gcgcgctcgc cccgcccctg taccgccggg cggtcggggg tggccggcgc
     3841 ttcacccccg gcaccgcgac gaacccggtg caggaagccg gacgggtgcc ggacggccat
     3901 gagccgcctg ccgggccggg gcccgcgagc tgaccgctca gcggagcagg ggccggagag
     3961 cggagtcgat ctgcgcgccg gtcaccggcc cctcgaagct ggcccggatg atccccttgc
     4021 ggtcgaccac gaccgtccac ggctcggtct ggaaccccca ggcggtgaac gcggccaccg
     4081 gcttgcccgg cgacttctgg tccgggtaga tctcgacgtg caggaggttc gcctgcggcg
     4141 ggcgcacctt gccggcgacg gtgagcagct cgtcgacgac cggcccgcac atgcggctcg
     4201 agcagaacag cggcgtcgcg aagcacacca ccgtgggcct gcccgacgcg agcgcccggt
     4261 ccagggagat ggcgtgcagg tcgcagggtg gcgtgcgggt gcagaccttg gccaggtcgg
     4321 cgcggtccgt ggccacgagc gtcgccgccg ccaccgcctt gctgccgatc cgcgccggca
     4381 cccggtccgc ggccaccggg acggcccccc tggccacgcc ctgctgcccg ccgctgtcga
     4441 cggtcgccgc gacgagccag ttgcccgccg ccgggaagtc gacccgggcg gcgtagaagc
     4501 cggtgaggtc cgaccttggc gaggtgtcgc ccgtcttctg gtaggcgtcg agcacgaacc
     4561 actcggcggt gaacgggccc gccgccctgc tggtctcgtc ccgcgccgtc cacacctggg
     4621 gccggccgcc ggtcacgagg cggttgtcgg gcgtggacag gccgaaggtg tagagctgcc
     4681 tgcccacctg gagcgtgctc tgggcgccga gcag
//
LOCUS       DASPYY010000120         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_160701, whole genome shotgun sequence.
ACCESSION   DASPYY010000120 DASPYY010000000
VERSION     DASPYY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160701"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VEP73_01790"
     CDS             <1..494
                     /locus_tag="VEP73_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYT25204.1"
                     /translation="DLQERLMPVIADHEAVVARAVRLAEAAALLDVPVRATEQNPAGL
                     GSTVPPLAGYPQSVLAKTTFSAAGDPGFAALLPGAGQIVVAGCEAHVCVLQTVLDLLA
                     AGRRVAWAADATGSRDPADRTAAIERARQHGADIVTSEMVLFEWLRDARHPRFREVHK
                     LLR"
     gene            491..1237
                     /locus_tag="VEP73_01795"
     CDS             491..1237
                     /locus_tag="VEP73_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HYT25205.1"
                     /translation="MSGYGEAMRAQGSGPGPLALVGSGEYLPVMAGVEGSLIEGRPRR
                     YVQIPTAAAPEGARRLQYWLDLGAAQAERLGAAQVPVVVRDRDEAESEDLAALVAGAG
                     LVYLSGGNPLFLARTLRGTRVWAAVQAAWRSGAALAGCSAGAIALTDWVPAVRAPAGE
                     AEPGLGALPWLRVLPHFDKMTSWAPDLVTRAAADAPPGTTVLGIDEDTAIVDLTGRGR
                     SWQVHGRQQAWVLDGGGPRRPFPAGATVQT"
     gene            complement(1296..2093)
                     /locus_tag="VEP73_01800"
     CDS             complement(1296..2093)
                     /locus_tag="VEP73_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT25206.1"
                     /translation="MSTVHSADGTAIAFDAWGGGQPLIMVDGATSYRAVSQLNAEVGK
                     LLGDQFRVYAYDRRGRGESGDTAPFAVQREVEDLAALIEEAGGTALVCGYSSGAILAL
                     DAAAAGLPITRLALFEPSFVVDGGRPPLPADYVARLDASTASGRPGDAVELFLTAAIG
                     MPAEAVAGMRQSPFWPALESVAHTIAYDGRIVGTTMSGAPLPADRWAAVSMPTLVMYG
                     RGTEPWLISAARALTDLLPAASLRAVDGEQHNVAAEVLAPVLREFGA"
BASE COUNT          300 a          856 c          864 g          284 t
ORIGIN      
        1 tcgacctgca ggagcggctc atgcccgtca tcgccgatca cgaggccgtc gtggcccggg
       61 ccgtgcggct cgcggaggcg gcggcgctgc tcgacgtgcc ggtccgcgcg accgagcaga
      121 acccggcggg cctgggctcg acggtgccgc cgctggccgg ctacccgcag tcggtcctgg
      181 ccaagacgac gttctctgcg gcgggcgacc cggggttcgc ggcgctgctc cccggcgccg
      241 gccagatcgt ggtggccggc tgcgaggcgc acgtgtgcgt cctgcagacg gtgctcgacc
      301 tgctggcggc cgggcgccgg gtggcctggg cggcggacgc gaccggctcg cgcgacccgg
      361 ccgaccgaac ggccgccatc gagcgggcgc gccagcacgg cgcggacatc gtgacgtcgg
      421 agatggtgct gttcgagtgg ctgcgtgacg cccggcaccc gaggttccgc gaggtgcaca
      481 agctgctcag gtgagcggct acggtgaggc catgcgggca cagggatcgg gtccagggcc
      541 gctggccctg gtggggagcg gtgagtacct gccggtcatg gcgggtgtcg agggctcgct
      601 gatcgagggg cgcccgcgcc gctacgtgca gatccccacc gcggccgctc ccgagggagc
      661 gcggcgcctg cagtactggc tcgacctggg cgcggcgcag gccgagcgcc tcggggcggc
      721 gcaggttccc gtggtggtca gggaccgcga cgaggccgag tccgaggacc tggcggccct
      781 ggtcgccggc gccggcctgg tctacctgtc cggcggcaac ccgctcttcc tcgcccggac
      841 cctgcgcggg acgcgggtct gggccgccgt ccaggccgcc tggcgctcgg gcgccgcgct
      901 ggcggggtgc tccgcggggg cgatcgcgct caccgactgg gtccccgcgg tccgcgcccc
      961 ggccggcgag gcagagcccg gtctgggcgc gctgccctgg ctgcgcgtcc tgccgcactt
     1021 cgacaagatg acctcctggg cgccggacct ggtcacgcgg gccgcggccg acgccccgcc
     1081 cggcaccacg gtgctcggga tcgacgagga caccgcgatc gtggacctga ccggccgcgg
     1141 ccgtagctgg caggtgcacg gccggcagca ggcctgggtc ctggacggcg ggggcccgcg
     1201 ccgcccgttc ccggccggcg ccaccgtcca gacctaacgc gtccggacct agcgcgtccg
     1261 gacctagcgc gtcccggacc ctggaccctt cagccctagg cgccgaactc gcgcaggacc
     1321 ggcgccagga cctcggcggc gacgttgtgc tgctcgccgt ccaccgcccg cagggacgcg
     1381 gcgggcagca ggtccgtcag cgcgcgggcc gccgagatca gccacggctc ggtgccgcgt
     1441 ccgtacatca ccagggtcgg catgctgacg gcggcccacc ggtcggcggg cagcggcgcg
     1501 ccggacatcg tcgtccccac gatccggccg tcgtaggcga tagtgtgggc gaccgactcg
     1561 agcgcgggcc agaacgggct ctgccgcatc ccggccacgg cctcggccgg catcccgatc
     1621 gcggcggtca agaacagctc caccgcgtcg ccggggcggc cgctggccgt ggacgcgtcg
     1681 agccgcgcga cgtagtcggc gggcagcggc gggcggccgc cgtcgaccac gaacgacggc
     1741 tcgaacagcg ccagcctggt aatcggcagc ccggccgcgg cggcgtccag ggcgaggatg
     1801 gcgcccgagg agtaaccgca gaccagcgcc gtccccccgg cctcctcgat cagggcggcc
     1861 aggtcctcga cctcgcgctg caccgcgaac ggagcggtgt cgccgctctc gccacgcccc
     1921 cggcggtcgt aggcgtacac ccggaactgg tccccgagca gcttcccgac ctcggcgttc
     1981 agctgcgaga ccgctcggta gctggtcgcg ccgtcgacca tgatcagcgg ctggcccccg
     2041 ccccacgcgt cgaaggcgat ggcggtgccg tccgcggagt gaaccgtgct catgtcatcc
     2101 tcccgtgtcc cgatcgggcc cctcccgacc gcatcttaag tactgaacgg taccgtacta
     2161 tgctcagtac ggtaccgtct agtactattc gggcagaacc tttggagaac gttcaccggg
     2221 agcacccgga gcaccgggaa ccaggagcac cgggaaccag aagcaccggg aaccagaagc
     2281 accgggaacc agaagcaccg ggaa
//
LOCUS       DASPYY010000121         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_863383, whole genome shotgun sequence.
ACCESSION   DASPYY010000121 DASPYY010000000
VERSION     DASPYY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863383"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VEP73_01805"
     CDS             <1..764
                     /locus_tag="VEP73_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25207.1"
                     /translation="VSDADNLVAGDGNGASDVFVYDRASGQTTLESRGSDGALADDWS
                     YYPAISADGRVVAFDSLATNLAANDGERPDVFVRDRASGQTRIASVSLTGGKADYPSF
                     APVLSADGRYVAFDSSASNLVAFDANGNGSDVFVRDLAAGTTALVSVDAGASAQPDQS
                     FDAAISPDGSFVAFAGLSVLSSGAADCQYYLRGPLRPPGQPDVPLAAPSTTVPPVETT
                     SLTTEAPSTSTSLSTEPPPAAPTSTLVPTTAPTVG"
     gene            956..2263
                     /locus_tag="VEP73_01810"
     CDS             956..2263
                     /locus_tag="VEP73_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HYT25208.1"
                     /translation="MRFGRVRHDDERGQVAIVVALTLTLMLAFGALIVDVGLNWAGRS
                     EVQSAADAASLAGAAKLPGSPDEAVALAKAYLFDNIPGLAGPPGWEGNGTDADGDITC
                     YRPPAPVPAPGAHGCQVGDTAIQVITPPLRPSYAFASILGRTSNEVKALAAAGATPGA
                     TAPCALCVLSTDQDPALVMNGTPQVSVEGAGVVVNSGSDIGNGSLTASNGTGSIGVAG
                     NARQGPQGQYSPAPTRGIAPIADPLSSLPAPNQLNPPVLNGPIAPLFPGVYETISVSG
                     GVTRRLQPGIYVIKGGISVSGGATLLASGVTLYLTCDSYPIAPCNGTGAGLDVSDGGR
                     LVLTAPVTTGPAAYRNLAIFFDRTNTAGLDIAASAGGSRVTGTIYAPSATLELGGDAA
                     TRLDSMTVVGAMSMQGGAQASAAIVYQPSQNVAVQAASGGLIK"
     gene            2420..2809
                     /locus_tag="VEP73_01815"
     CDS             2420..2809
                     /locus_tag="VEP73_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25209.1"
                     /translation="MLFSNLLVAIVLTTLWPLGLLGVLAACAWLERRTLVTGEVLPRR
                     IQRMERKPPEAVEAMVLEETAEIVAAYWSSTGRPLPARAGPAENGQRPAATAAQAPAS
                     GQGRPVPGRADRAAMRRRPSRGRHERR"
     gene            2929..3204
                     /locus_tag="VEP73_01820"
     CDS             2929..3204
                     /locus_tag="VEP73_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYT25210.1"
                     /translation="MGIIALLVLGLLAGAVAKLLLPGPDPGGIIITMAIGVVGALLGG
                     FLAAALGFGGLSGFDFRTFVIAVIGSIVLLLIYRAFAGRGRGRRRLI"
     gene            3353..>3586
                     /locus_tag="VEP73_01825"
     CDS             3353..>3586
                     /locus_tag="VEP73_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25211.1"
                     /translation="MEQRVARRLRTSEAFVAGVSAMLVATGVLGLAHIVEGSVPFPSL
                     GLAQRLLRVVPGPLAVFFVERLGHLALKLFAIGF"
BASE COUNT          451 a         1379 c         1250 g          506 t
ORIGIN      
        1 tcgtgtccga cgccgacaac ctcgtggccg gcgacggcaa cggcgcgagc gacgtgttcg
       61 tctacgaccg ggccagcggg cagacgacgc tggagtcgcg cggctccgac ggcgcgctcg
      121 ccgacgactg gagctactac ccggcgatct cggccgacgg ccgggtcgtg gccttcgact
      181 cgctggcgac caacctcgcc gccaacgacg gggagcggcc ggacgtcttc gtgcgcgacc
      241 gggccagcgg gcagacgagg atcgcctcgg tgagcctgac cggcgggaag gccgactacc
      301 ccagcttcgc cccggtcctg tcggccgacg gccgctacgt ggcattcgac tcgagcgcct
      361 ccaacctggt ggccttcgac gccaacggca acggcagcga cgtgttcgtc cgcgacctcg
      421 cggccgggac gaccgcgctg gtctcggtcg acgccggcgc gtcggcacag cccgaccagt
      481 ccttcgacgc cgccatctcg cccgacgggt cgttcgtggc gttcgcgggc ctttccgtgc
      541 tgtcgagcgg cgccgccgac tgccagtact acctgcgggg cccgctgcgc ccgcccggcc
      601 agccggacgt tcccctggcc gctccgtcca ccaccgtgcc gccggtcgag acgaccagcc
      661 tgaccaccga ggcgccgtcc acctccacca gcctgtcgac ggagccgccc ccggcggcgc
      721 ccacctccac cctcgtcccc acgaccgcgc cgacggtcgg ctgagccgct ccgccgcctg
      781 ccgtcgcgcc caccgagtcc gcagcatccg tctcgtccgc gacgaatgtg tcatacggcg
      841 gggacttggt cacgagcgga agatcggcga ccatgtcccc ggcgccacgg gtcccgtggc
      901 caccgcatcg gcgtgagcga gcaccggtcg tccaccctca cccgagaagg attcgatgcg
      961 tttcggtcga gtacgccacg acgacgaacg cggccaggtc gccatcgtcg tcgccctcac
     1021 gctgacgctc atgctggcgt tcggcgcgct gatcgtggac gtggggctca actgggccgg
     1081 ccgcagcgag gtccagagcg ccgccgacgc cgcgtcgctg gccggagcgg cgaagctgcc
     1141 cggctccccg gacgaggcgg tcgcgctggc caaggcctac ctgttcgaca acatccccgg
     1201 cctggccggc cccccgggct gggagggcaa cggcaccgac gccgacggcg acatcacctg
     1261 ctaccggcca ccggccccgg tgccggctcc gggcgcccac ggctgccagg tcggtgacac
     1321 cgccatccag gtgatcacgc cgccgctcag gccgtcgtac gccttcgcga gcatcctcgg
     1381 gcgcacctcg aacgaggtga aggcgctggc ggcggccggg gcgacacccg gggccaccgc
     1441 gccgtgcgcc ctctgcgtgc tctcgaccga ccaggaccct gcgctcgtca tgaacggcac
     1501 tccccaggtc tccgtggagg gcgccggcgt cgtggtcaac tcgggcagcg acatcggcaa
     1561 cggcagcctc accgccagca atggcaccgg ctcgatcggc gtcgccggca acgcgcggca
     1621 gggcccgcag ggccagtact cgcccgcgcc gacgaggggg atcgccccga tcgccgaccc
     1681 gctctccagc ctcccagcgc ccaaccagct caacccgccg gtcctgaacg gaccgatcgc
     1741 gccgttgttt cccggcgtgt acgagaccat cagcgtgagc ggcggcgtca cgaggcggct
     1801 gcagcccggg atctacgtca tcaagggcgg catcagcgtc tccggagggg cgacgctcct
     1861 cgccagcggc gtcacgctgt acctcacctg cgacagctac cccatcgcgc cgtgcaacgg
     1921 cacgggagcg ggcctcgacg tctcggacgg cggcaggctc gtcctcacgg ccccggtcac
     1981 gaccggcccg gccgcctacc gcaacctggc catcttcttc gaccggacca acacggccgg
     2041 gctcgacatc gccgccagcg ccggcggcag cagggtcacc ggcacgatct acgccccgag
     2101 cgcgacgctc gagctcgggg gggacgcggc gacgaggctc gactcgatga ccgtcgtcgg
     2161 cgccatgagc atgcaagggg gtgcgcaggc gtcggccgcc atcgtctacc aacccagcca
     2221 gaacgtcgcg gtccaggccg cgagcggcgg gctgatcaag tgacatgaac cggggacgcg
     2281 cgggaggccg ggcaggatcc gcggcggcgt tgcggcccgc cctgaccgcc ggtgcgcgtc
     2341 cggggcaccg gccgagtttg cggccgccct ccgtggtcaa gccgtaccag gtcctccgtg
     2401 gtcgttcgtg aggtcgagca tgctgttctc caacctcctg gtcgcgatcg tgctcaccac
     2461 gctgtggccg ctcgggctgc tcggcgtgct ggcggcgtgc gcctggctgg agcggcgcac
     2521 cctggtcacc ggggaggtcc ttccccggcg gatccagcgg atggagcgca agccgcccga
     2581 ggcggtcgag gcgatggtcc tcgaggagac ggccgagatc gtcgccgcct actggtcgtc
     2641 gaccggccgc cccctgccgg cccgcgccgg tccggccgag aacggccagc gtcccgcggc
     2701 cacggccgcg caggcgccag cctccggcca ggggcggccg gtgcctgggc gggccgacag
     2761 ggccgccatg cggcgcaggc cctcccgcgg ccgccacgag cggcgctgac gcgctcccga
     2821 gtccctgttt gccgcatgcc ccgtgcggga agcctaccta tgtgggttgg cttgcttcct
     2881 tcgtcgcacc cgaaacggtc tgcgacgtca cccgaaagga gcacgttcat ggggatcatc
     2941 gccctgctgg tgctgggcct tctcgccggc gccgtcgcca agctcctcct tcccggcccc
     3001 gacccgggcg ggatcatcat caccatggcc atcggcgtcg tgggcgcgtt gctcggcggg
     3061 ttcctcgccg cggcgctcgg cttcgggggg ttgtcggggt tcgacttccg gaccttcgtc
     3121 atcgccgtga tcggctcgat cgtgctgctg ctcatctacc gggcgttcgc cgggcgtggc
     3181 cgaggacggc ggcgcctcat ctgaccttcg acgctcgcgg cgcggcccgg cggggccgcg
     3241 ccgctcgttc gtgccgccgg tcccgcggcc ttcgcccggt cttccgcggc gggccaccga
     3301 acctcgggct tgcggttatc gtggacccat ggaacgggtg gaacggcaat ccgtggaaca
     3361 gcgcgtcgcg cgacggctgc ggacgtcgga ggcgttcgtc gcgggggtct cggcgatgct
     3421 ggtcgcgacc ggggtactcg gcctcgccca catcgtcgag gggagcgtcc cgttcccttc
     3481 cctcggcttg gcccagcgcc tgctgcgggt cgtccccggg ccgctcgcgg tcttcttcgt
     3541 cgagcggctg ggccacctgg ccctgaagct gttcgcgatc ggcttc
//
LOCUS       DASPYY010000122         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1004379, whole genome shotgun sequence.
ACCESSION   DASPYY010000122 DASPYY010000000
VERSION     DASPYY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004379"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VEP73_01830"
     CDS             <1..1069
                     /locus_tag="VEP73_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HYT25212.1"
                     /translation="RYPVLLGGAALNRTYVEGDLRRLYKGRVFYCRDAFAGLAVMDQL
                     REERASGVKVEKEAVRAPRVTPRAPRGPAPEAPGTSETARSRVATDVAVPTPPFLGEA
                     RIVKGIPVRDVAPYLNERALFRGQWQLRPAGGRDGWERVLETEARPRLRALLDEAVAR
                     QILRPAIVYGYFPCNADGNDLVVWRQDGSEWVRFTFPRQRRDERLCLADYFRPVDSGE
                     RDVVGFQVVTMGASASRATAALFAADRYREYLELHGVTVEMAEALAEYWHKRVREELG
                     IAGQDAADVEELFDLGYRGARFSFGYGACPDLEEQTKIFELLHPERIGVELSEEFQLH
                     PEQSTSAVVVHHPEAKYFNAR"
     gene            1150..2451
                     /locus_tag="VEP73_01835"
     CDS             1150..2451
                     /locus_tag="VEP73_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYT25213.1"
                     /translation="MAPTTEQLFVSRLARLPVRAPNGDTIGRINDVVVAKAVPGSPAV
                     VLGMTVVVQRHPIFVAMGRVRELGEDGATLRTGSVNLRPFELRQGESLVIGELLDAKV
                     RHRETGKELRVNDVAISQVRHEGWVITALDVLEPTGGLRPWRRARHLRLPWDMVAGLE
                     AAETAESRVAALAGLRPADLAAVLQSMPARVQRDVVHDLDDDRLAEALEELPEEVQAG
                     LLAGLEEERAADVLEAMDPDDAADLLGELSEADRARLLALMVPDEAEPVRRLLVYDEE
                     TAGGLMTTEPVIMRASDTVAEALARIRDPDLPPALAGQVFVVRPPTQTPTGPYMGVAH
                     LQRLLRELPGDRLVSCIDTDLDTLRPDLPTRRVAEYLATYDLLAAPVCDDRGRLLGAV
                     SVDDVLDHLLPEAWRRGPVRTRGNGNGPRQPVAEQAGEERP"
     gene            2490..2984
                     /locus_tag="VEP73_01840"
     CDS             2490..2984
                     /locus_tag="VEP73_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYT25214.1"
                     /translation="MAAPRQRRAPRIQSQYYDPDAFGRFSEAIARFLGTARFLVLQTG
                     VVVAWIAWNVALRKEGLSFDPYPFILLTLALSLQASYAAPLILLAQNRQEERDRQETE
                     TDRRTNTRALADTEYLARELASIRLALGEMPTRDYLDEELEPIKEELSRLRARLEQAA
                     RAED"
     gene            2988..3407
                     /locus_tag="VEP73_01845"
     CDS             2988..3407
                     /locus_tag="VEP73_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HYT25215.1"
                     /translation="MLRTNLLHPEILHALGSAGHGSTVLLADGNFPHRTAPSPGAPRV
                     YLNLAPGVVTVTQVLAAVRSVIEVERAAVMLPDDGTRLAVHEEFARLLDGTPLDAMGR
                     FEFYDATRTGDLALVVATADERVYANLLLTVGVVAPG"
     gene            complement(3419..4114)
                     /locus_tag="VEP73_01850"
     CDS             complement(3419..4114)
                     /locus_tag="VEP73_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25216.1"
                     /translation="MPRPSLPPSRSGPFLLCWLLMAALVSAGCQAFPSAKADEQTSAS
                     TTTATPAGAVPLATSCDQSPLPRHDGFQDAPRCVAVDDGEVPALTPAVLIARFPRLVR
                     AGRQFQVTISTRNLLRDRFLAAAAGGYYREMSVLDPGSGVVRGHLHAYVQALAGGVVP
                     DAEAAPLEFFVAIEDGGGGAGVSSVTVSVNGLTAGRKRLCVTAGDGSHRVPMMRAAKL
                     AAAIDCVRIRVIR"
BASE COUNT          563 a         1563 c         1559 g          533 t
ORIGIN      
        1 ccgctacccc gtcctgctcg gcggggccgc cttgaaccgc acctacgtcg agggcgacct
       61 gcggcgcctc tacaagggcc gcgtcttcta ctgccgggac gcgttcgcgg gcctcgcggt
      121 gatggaccag ctccgcgagg agcgggcgag cggcgtcaag gtcgagaagg aggcggtgcg
      181 ggcaccccgg gtcaccccca gggcgccgcg cggcccggcc cccgaggcgc cgggcacgag
      241 cgagaccgcc cgctcgcggg tggccaccga cgtggcggtc ccgaccccgc ccttcctcgg
      301 cgaggcgagg atcgtcaagg gcatccccgt gcgcgacgtc gctccctacc tgaacgagcg
      361 ggcgctgttc cgcggccagt ggcagctccg cccggcgggt gggagggacg gctgggagcg
      421 cgtcctcgag accgaggccc ggccgcgcct tcgcgccctg ctcgacgagg cggtcgcccg
      481 gcagatcctc cgcccggcga tcgtctacgg gtacttcccg tgcaacgcgg acggcaacga
      541 cctcgtggtg tggcgccagg acggcagcga gtgggtgcgc ttcacgttcc cgcgccagcg
      601 gcgcgacgag cgcctgtgcc tcgcggacta cttccggccg gtcgactccg gggagcgtga
      661 cgtcgtcggc ttccaggtcg tgacgatggg cgcctccgcg tcgcgggcga ccgccgcgct
      721 gttcgccgcc gaccgctacc gcgagtacct cgagctgcac ggcgtcaccg tcgagatggc
      781 cgaggcgctg gccgagtact ggcacaagcg cgtccgcgag gagctcggca tcgcgggcca
      841 ggacgccgcc gacgtggagg agctgttcga cctcgggtat cgtggcgccc gcttctcgtt
      901 cggctacgga gcttgccccg acctcgagga gcagaccaag atcttcgagc tcctccaccc
      961 ggagcgcatc ggggtcgagc tgtccgagga gttccagctc caccccgagc agtcgacctc
     1021 ggccgtggtc gtgcaccatc ccgaggcgaa gtacttcaac gcgaggtagc cccggcgggg
     1081 gggagcaacc caggatgccg gtggtaccca ggatgccggt gtccgacccg tgccgggagg
     1141 tgagcaagcg tggccccgac gaccgagcag ctcttcgtgt cccgcctggc gcggctgccg
     1201 gtacgggccc cgaacgggga cacgatcggt cggatcaacg acgtggtggt cgccaaggcc
     1261 gtccccggct cgcccgcggt cgtcctcggc atgacggtcg tggtccagcg ccacccgatc
     1321 ttcgtcgcca tgggccgggt cagggagctc ggcgaggacg gcgccaccct gcgcacgggc
     1381 tcggtcaacc tgcggccgtt cgagctcagg cagggggagt cgctcgtcat cggggagctg
     1441 ctcgacgcca aggtccgcca ccgcgagacg ggcaaggagc tccgtgtcaa cgacgtggcg
     1501 atcagccagg tccgccacga gggctgggtg atcaccgcgc tcgacgtgct ggagccgacc
     1561 ggcgggctgc gcccctggcg ccgggcgagg cacctgcgcc tgccctggga catggtggcc
     1621 gggctggagg cggccgagac ggccgagagc cgcgtcgccg ccctcgccgg cctgcgtccc
     1681 gccgacctgg ccgccgtgct gcagtccatg cccgcgcggg tccagcggga cgtggtccac
     1741 gacctcgacg acgaccggct ggcggaggcc ctggaggagc tccccgagga ggtccaggcc
     1801 ggcctgctcg ccgggctgga ggaggaacgc gcggcggacg tgctcgaggc gatggacccc
     1861 gacgacgccg ccgacctcct cggcgagctg tcggaggcgg accgggcccg gctgctcgcc
     1921 ctcatggtcc ccgacgaggc cgagccggtg cggcgcctgc tcgtctacga cgaggagacg
     1981 gcgggcggcc tcatgaccac cgagccggtc atcatgcggg cgtccgacac ggtcgccgag
     2041 gcgctggccc ggatccgcga ccccgacctg ccgcccgccc tggccggcca ggtcttcgtg
     2101 gtgcggcccc cgacgcagac cccgaccggc ccgtacatgg gcgtggcgca cctgcagcgg
     2161 ctgctgcgcg agctgccggg cgaccggctc gtgtcctgca tcgacaccga cctcgacacc
     2221 ctgcggccgg acctgcccac ccggcgggtg gcggagtacc tggcaaccta cgacctgctc
     2281 gccgcgccgg tctgcgacga ccggggccgg ctcctcgggg cggtgtcggt cgacgacgtt
     2341 ctcgaccacc tgctgcccga ggcgtggcgg cgcggcccgg tccgcacccg cggcaacggc
     2401 aacggcccgc ggcagccggt ggccgagcag gcgggggagg agcggccgtg agcagccgga
     2461 cggcgaaggg acggcgccgg gcgtcgcggc tggcagcgcc ccgccagcgg cgggcgccca
     2521 ggatccagtc gcagtactac gacccggacg cgttcggccg cttctccgag gcgatcgccc
     2581 gcttcctcgg caccgccagg ttcctggtgc tgcagaccgg ggtggtggtc gcctggatcg
     2641 cctggaacgt ggcgctccgc aaggagggcc tctcgttcga cccctacccg ttcatcctgc
     2701 tcaccctggc gctgtcgctg caggcgtcct acgccgcccc gctcatcctg ctcgcccaga
     2761 accgccagga ggagcgggac cgccaggaga ccgagaccga ccggcgcacc aacacccggg
     2821 cgctcgcgga caccgagtac ctcgcgcggg agctggcctc gatccggctc gccctcggcg
     2881 agatgccgac ccgcgactac ctcgacgagg agctggagcc catcaaggag gagctcagcc
     2941 ggctgcgggc ccggttggag caggcagccc gcgcggagga ctgacccatg ctgcgtacga
     3001 acctgctcca cccggagatc ctgcacgccc ttggctccgc cgggcacggc tccacggtcc
     3061 tgctcgcgga cggcaacttc ccgcaccgga cggcgccgtc cccgggcgcc ccgcgggtgt
     3121 acctcaacct ggcgcccggc gtggtcaccg tcacccaggt cctggccgcg gtccgctcgg
     3181 tgatcgaggt ggagcgggcg gcggtgatgc tccccgacga cgggacccgg ctcgcggtcc
     3241 acgaggagtt cgcgcggctg ctggacggca ccccgctcga cgccatgggc cggttcgagt
     3301 tctacgacgc gacgcggacc ggcgacctcg ccctcgtggt ggccaccgcg gacgagcggg
     3361 tctacgccaa cctgctgctg accgtcgggg tcgtcgcacc ggggtgaccc cggccgggct
     3421 accggatcac ccggatccgg acgcagtcga tggcggcggc gagcttggcc gcccgcatca
     3481 tcggcacccg gtgcgacccg tcccccgcgg tcacgcacaa ccgcttccgc cccgcggtca
     3541 acccgttcac cgacacggtc accgacgaca cccctgcccc gccgccgccg tcctcgatcg
     3601 ccacgaagaa ctcgagcggc gccgcctcgg cgtcgggcac cacaccaccg gcaagcgcct
     3661 gcacgtaggc gtgcaggtgg ccgcggacca ccccggagcc cgggtcgagg acgctcatct
     3721 ccctgtagta gccgccggcg gcggccgcga ggaagcggtc acggagcagg ttgcgggtgc
     3781 tgatggtgac ctggaactgc cggccggccc gcaccaggcg ggggaagcgg gcgatcagca
     3841 cggcgggggt gagggcgggg acctcgccgt cgtccacggc gacgcagcgg ggggcgtcct
     3901 ggaagccgtc atggcgtggc agggggctct ggtcgcagct ggtggccagc ggcacggccc
     3961 cggcgggcgt cgcggtcgtc gtcgaggcgc tcgtctgctc atcggccttc gccgacggga
     4021 aggcctggca gccggcgctc acgagcgccg ccatgagcag ccagcagagc aggaacggcc
     4081 cgcttcggga cggcggaagg cttggtcgtg gcatacgccg accacctttc atgggggatg
     4141 cagcctgttc gggctgcacg aaaggacgca tacaaggccg gccgcgtgat gggcgacgag
     4201 cctgaggtat gcaggctg
//
LOCUS       DASPYY010000123         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1986838, whole genome shotgun sequence.
ACCESSION   DASPYY010000123 DASPYY010000000
VERSION     DASPYY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986838"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VEP73_01855"
     CDS             complement(<1..1260)
                     /locus_tag="VEP73_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYT25217.1"
                     /translation="MTATPSGEAPFDLDAFVRLPRVSGLALSPDGTRLVASVATLAPD
                     GKGFVTSLWELDPDGARPPRRLTRSAPGESGAAFLPDGSLLFTSSRKDPDAKPEERED
                     DDKAAIWLLPPGGGEARLVASAPGGVHALEVAREAGTVVYATSLHPDAETLDEDERRA
                     KAARDAGVGAQLFEGYPIRYWDHYLGPRAPRLFAATPPHDDRLGAGVQLTPAPGRALD
                     EASFAVSPDGATVLTTWKVDDVHDPRTQLVAIDVAGGAQRRLFDEPEASVDEVAISPD
                     GRFAVCQRIAHGTPTTGPSVTLWLVDLATGGGRDLTPDFEPWPSGPAWAPDGSAVFFV
                     ADEGGRTPLFRVEVASRASGIRASPDGGVTRLTAEGTYSDCRPAPDGRRLYALRSTVA
                     SPPEAVVLDAATAGQEPRPLPTPGLPLL"
     gene            complement(1285..2100)
                     /locus_tag="VEP73_01860"
     CDS             complement(1285..2100)
                     /locus_tag="VEP73_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYT25218.1"
                     /translation="MSIRFRYVGHAALLAGGAMVTTLAAEVWAAMHHKFVPSTPVLEI
                     SGVIGPEDGEPLTVAVLGDSSVAGVGADSAEDTLAYGVAKALADRYRVSLHALGVSGS
                     RLRNVVGEQLPQLAGLDPDVVLICVGTNDITHSTTTREIRGHLKQLGAGLRTVAPRAV
                     VVVSGLPPAAVALCFRWPLRNLLGVRAWAVTRIYKSELAAFGIPVFEIARETRAAFRG
                     RREMFSADLFHPSSAGYAFLGTVYGRAVREALDTARAEPRPGTDLGLADGLAG"
BASE COUNT          319 a          921 c         1023 g          308 t
ORIGIN      
        1 gaggagcggg agccccggcg tcggcagcgg ccggggctcc tggccggccg ttgccgcgtc
       61 gagcacgacc gcctccggcg gggaggcgac ggtggaccgc agggcgtaga ggcggcgccc
      121 gtccggtgcc gggcggcagt cgctgtaggt gccctcggcc gtcagccggg tgacccctcc
      181 gtcaggtgac gcccggatgc ctgaagcgcg tgaggccacc tcgacccgga acagcggggt
      241 gcggccaccc tcgtcggcga cgaagaacac cgccgagccg tcgggcgccc aggccgggcc
      301 ggagggccag ggctcgaagt ccggggtcag gtcgcggccc ccgccggtcg cgaggtcgac
      361 gagccacagc gtcaccgacg gtcccgtcgt gggcgtcccg tgggcgatgc gctggcagac
      421 cgcgaagcgc ccgtccgggg agatggccac ctcgtcgacg gacgcctccg gctcgtcgaa
      481 cagcctgcgc tgagcgccgc cggcgacgtc gatcgcgacc agctgggtgc gcgggtcgtg
      541 gacgtcgtcg accttccagg tcgtgagcac cgtggcgccg tccggcgaca ccgcgaagct
      601 cgcctcgtcc agggcgcggc cgggcgccgg ggtgagctgc acgccggccc ccaggcggtc
      661 gtcgtgcggg ggagtggcgg cgaacagccg cggcgcgcgc ggcccgaggt agtggtccca
      721 gtagcggatc gggtagccct cgaagagctg ggcgccgacg ccggcgtccc tggcggcctt
      781 ggcccggcgt tcgtcctcgt ccagggtctc ggcgtccggg tggaggctgg tcgcgtagac
      841 gacggtgccc gcctcgcggg ccacctcgag cgcgtggacg ccgcccgggg ccgacgcgac
      901 caggcgggcc tcgccgccgc cgggcggcag cagccagatc gccgccttgt cgtcgtcctc
      961 gcgctcctcg ggcttggcgt cagggtcctt gcgggacgac gtgaacagca gggagccgtc
     1021 ggggaggaac gcggcgccgc tctcgccggg ggcggagcgg gtcagccggc gtggcggccg
     1081 cgccccgtcc gggtcgagct cccagaggga ggtcacgaac cccttgccgt cgggcgccag
     1141 cgtggccacc gaggcgacca ggcgcgtccc gtccggcgag agcgcgagcc ccgacacgcg
     1201 cggcagcctc acgaaggcgt cgaggtcgaa gggggcttcg ccgctgggcg tggcggtcat
     1261 ggggcggcct ccccgggccg gtggtcagcc cgcaaggccg tcggcgaggc cgaggtcggt
     1321 tcctggacgc ggctcggccc gggccgtgtc gagggcctcg cggaccgctc ggccgtagac
     1381 cgtgcccagg aaggcgtagc cggcgctcga cgggtggaac aggtcggcgc tgaacatctc
     1441 gcgcctgccc cggaaggcgg cccgggtctc gcgcgcgatc tcgaacacgg gaatgccgaa
     1501 tgcggccagc tcggatttgt agatgcgcgt gaccgcccac gcccgcacgc ccagcagatt
     1561 gcgcagcggc cagcggaagc agagcgcgac cgcggcgggg ggcaggccgg agacgaccac
     1621 cacggcgcgc ggggcgaccg tgcgcagccc ggccccgagc tgcttcaggt ggccgcggat
     1681 ctccctggtc gtggtggagt gggtgatgtc gttggtgccg acgcagatca gcaccacgtc
     1741 cgggtcgagg ccggcgagct gcgggagctg ctcgcccacg acgttgcgga gccgggagcc
     1801 ggagaccccg agcgcgtgga ggctgacccg gtagcggtcg gccagcgcct tggcgacccc
     1861 gtacgcgagc gtgtcctcgg ccgagtcggc gccgaccccg gctaccgagc tgtcgccgag
     1921 caccgccacc gtcagcggct cgccgtcctc ggggccgatg acaccgctga tctcgagcac
     1981 gggcgtggag ggaacgaact tgtggtgcat ggccgcccag acctcggcag ccagggtcgt
     2041 gaccatcgcc ccgccggcga gcaacgcggc gtggccgacg taccggaacc ggatgctcat
     2101 cgtcccctcc tcacccagag cgtgcgagtt ctcgtccggt atggggagga ccggcgcgct
     2161 tgggaggaat cggcgggcgc cggcgccgcc gagggtggga gggcgggagg ggttcctcct
     2221 ccccggcggg cgggagcggg gtcatccgcg cgctccgcgt ccgctccgga aggcggcaag
     2281 cccggtccgt ccggccgacc ggagccttgt gtgccgcgga ccgatacggt gccggagcgg
     2341 gtcggatgcg ctcgccatcg tgccctcagc ttcgactgtc tggttccccc gtctcaagtg
     2401 taaccaggcc gtcgcgccac cgggagcccg atttgtgccc ggaggcatcg cggcccggac
     2461 gggggcccga ccgggaggga ccggggggtg gcccgggtgg cccgggacac cgccccgcgc
     2521 ataccatccg cccatgaccg aatcctccgg tgcgcccacc gtccggcggc g
//
LOCUS       DASPYY010000124         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1144906, whole genome shotgun sequence.
ACCESSION   DASPYY010000124 DASPYY010000000
VERSION     DASPYY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144906"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..114
                     /locus_tag="VEP73_01865"
     CDS             4..114
                     /locus_tag="VEP73_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYT25219.1"
                     /translation="MSAHTVKTHLERIFDKLGANDRAQAVAIALRKGLVD"
     gene            complement(134..1432)
                     /locus_tag="VEP73_01870"
     CDS             complement(134..1432)
                     /locus_tag="VEP73_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25220.1"
                     /translation="MRGPDGDPGLVIDAVAAGYGGRAVLDRVSLSAGPGELVGLIGPN
                     GSGKTTLVRVASRGLRPTSGSVRAHGVDPYAISSREAARLVAVVPQELAPAFAYTVLE
                     VVLMGRSPYRSPWSGDGGEDWARAREAMATANVQHLADRPIAELSGGERQRTVLAQAL
                     AQDAPILLLDEPTTHLDPRHVLDALLVVRGLVRERGRAALAIFHDLNLASAFCDRLYV
                     LREGRVVAEGTPRAVITRDLLREVYGVDADVTTNPGTGRPSVTLAPPPEAEPAMAGLR
                     RAHVIGGAGRGADLMRELAELGFEVTAGVLHATDTDAVVADRLNLRRVVVPAFSVVDE
                     AATAECMELVRSASVVVVADPPFGPGNVANLRLARSASDEGVPLVLLETSPIGGRDFT
                     GGQASELWSALAQRGRVAASDREVLALVRDVGLRNPTRPA"
     gene            complement(1419..>2209)
                     /locus_tag="VEP73_01875"
     CDS             complement(1419..>2209)
                     /locus_tag="VEP73_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HYT25221.1"
                     /translation="GAVFQALFRNPLADPAIIGVSSGAALGAIVVIVSGAGAALGGLS
                     IPGAAFIGALGTAVLVYRLARVGPAIQVATLLLAGIAVAAVISSVISLVMTFSGQEVR
                     SIYFWLLGGLEAATWRQLAIGAPVMLVGMSAAMFSTGDLNLSALGEERAAQLGLEVAR
                     FKRTMLATGSLLAAVAVSVSGLIGFVGLMTPHILRLVVGSDHRRLVPASALGGATFMV
                     LADLVARTAVRPEEIPVGAVTAVLGGPFFLYLLRRERRAAGARA"
BASE COUNT          346 a          859 c          769 g          235 t
ORIGIN      
        1 gggatctcgg cgcacacggt gaagacgcac ctcgagcgca tcttcgacaa gctcggggcg
       61 aacgaccgcg cgcaggccgt ggccatcgca ctccgcaagg gcctggtcga ctgacggagc
      121 ggccggtcct gattcaggcc ggtctcgtcg ggttccgcag cccgacgtcg cggaccagcg
      181 ccagcacttc ccggtcggag gccgccaccc gcccgcgctg cgccagggcc gaccacagct
      241 ccgacgcctg cccgccggtg aagtcccgcc cgccgatcgg cgacgtctcc agcagcacca
      301 gcggcacgcc ttcgtcggac gcgctccgcg ccaggcggag gttcgccacg ttgcccggcc
      361 cgaacggagg gtcggccacg accaccacgg acgcgcttcg aaccagctcc atgcactccg
      421 cggtggcggc ctcgtccacc acggagaagg ccgggaccac cacccgacgg aggttcaagc
      481 ggtcggcgac gaccgcgtcg gtgtcggtgg cgtggaggac gccggccgtg acctcgaagc
      541 cgagttcggc cagctcgcgc atcaggtcgg ccccacgccc ggcacccccg atcacgtggg
      601 cccgccgaag cccggccatg gccggctcgg cctccggcgg tggggcgagc gtgacggacg
      661 gacggccggt gcccgggttc gtcgtgacgt cggcgtcgac tccgtagacc tcccggagga
      721 gatccctggt gatgaccgcg cgaggggttc cctccgccac gacacgccct tcccgcagca
      781 cgtacaggcg gtcgcagaac gcggaggcga ggttcaggtc gtggaagatg gcgagcgccg
      841 cccggccccg ctcgcggacc agcccccgga ccaccagcag ggcgtccagc acgtggcgcg
      901 ggtccaggtg ggtggtcggt tcgtccagca ggaggatcgg ggcgtcctgg gcgagggctt
      961 gcgccaggac cgttcgctga cgttctccgc ccgacagctc cgcgatcggg cggtcggcca
     1021 ggtgctggac gttcgccgtg gccatggcct cgcgggcccg ggcccagtcc tcgcccccat
     1081 ccccgctcca cggggagcgg tacggcgagc gacccatcag gaccacctcc aggacggtgt
     1141 aggcgaaggc cggagcgagc tcctgcggca cgacggcgac caggcgagcg gcctcgcgcg
     1201 aggagatcgc gtacggatcc acgccgtgcg cccggacgga gccgctggtg ggccggaggc
     1261 ctcgagaggc cacgcgcacc agggtcgtct tgccggaccc gttcggcccg atcaagccga
     1321 ccagctcgcc cggtcccgcc gagagcgaga cgcggtccag caccgctcgg cctccgtacc
     1381 cggcggcgac cgcgtcgatc accagcccgg ggtcaccgtc aggcccgcgc accggccgcc
     1441 ctccgctcgc gccgcaacag gtacaggaag aacggcccac ccagaacggc cgtcacggct
     1501 cccaccggga tctcctccgg ccggacggcc gtccgggcca ccaggtcggc gagcaccatg
     1561 aaggtcgcgc cgcccagcgc cgacgccggg accagccgcc gatggtcgga ccccaccacc
     1621 agccgcagga tgtgtggagt catcagcccg acgaacccga tcagcccgga gaccgacacc
     1681 gccacggcgg cgagcagcga gcccgtcgcg agcatcgtcc gcttgaagcg agccacctcg
     1741 aggccgagct gcgccgcccg ctcctccccc agcgccgaga ggttcaggtc cccggtggag
     1801 aacatcgcgg ccgacatccc gacgagcatc accggggcgc cgatcgcgag ctggcgccac
     1861 gtcgcggcct ccagcccccc cagcagccag aagtagatcg accgcacctc ctggccggag
     1921 aacgtcatca ccagcgagat gacggaggag atgacggccg ccaccgcgat gcccgccagc
     1981 agcaacgtgg cgacctggat ggcgggcccc acccgggcca gccggtagac gaggactgct
     2041 gtgcccagcg ctccgatgaa cgccgccccc gggatcgaca ggccccccag cgccgcgccg
     2101 gccccggaga cgatcaccac gatggccccg agcgccgccc cggatgacac cccgatgatc
     2161 gccgggtcgg cgagcgggtt gcggaacagg gcctggaaca ccgcacccg
//
LOCUS       DASPYY010000125         1789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_301358, whole genome shotgun sequence.
ACCESSION   DASPYY010000125 DASPYY010000000
VERSION     DASPYY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301358"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1255)
                     /locus_tag="VEP73_01880"
     CDS             complement(182..1255)
                     /locus_tag="VEP73_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT25222.1"
                     /translation="MPAAPVPFALVGATVLDGSGGPPLPGHTVVVRDGRVHAVGPREE
                     LAPGEGIRAIPAAGLTVLPGFVDTHVHLDLHAPAAVLLGGVTTVRDLGWPAERLAVLE
                     REAAAPVPVSPHLLRAGQILTAPGGYPTRAAWAPPGTARPVAGPAEAVRAVGELAEGR
                     AAVVKVALDGRAGPTMPEATLAAVVAAARGRGLEVTAHVAGVREVEKALAAGVAELAH
                     WPFTARPLPVTLCEALASSMTVVPTLHIEPTRARRQGLATFLAAGGRVVYGTDLGNKG
                     PPPGIDVEELRLMVEAGMSPGEALVAATAGAADHLGLAGAGRVAPGARAALIAVRGDP
                     LRDLRALTRVQLVSRAGAVAAAG"
     gene            1338..>1789
                     /locus_tag="VEP73_01885"
     CDS             1338..>1789
                     /locus_tag="VEP73_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYT25223.1"
                     /translation="MTAADGELRFDPIVLDGPPDGAVPLELGRRVALYVCGITPYDTA
                     HLGHAFTYVAFDTLVRFLRRRGHEVDYCQNVTDVDDDVLARAARNGEDYLALGRRETA
                     AFLEVMDALNVARPTWSPRATEEIGPMVDLAARLVGEGHAYEVDGTVF"
BASE COUNT          217 a          667 c          705 g          200 t
ORIGIN      
        1 gcggtggcga cggcgaaatc gcccgacggc gcgccggccg ggaggggacg cgcaaaacca
       61 ccgttcgcca ggcgggcggc cgtgccggtt tgccgtaccg tccaattgcg cggcccgcga
      121 cgtgccgggc cgtccagcgc gcctccacgt gccgtgcggg ccggtgcgcg tccagacagc
      181 gtcacccggc cgccgcgacg gcgccggcgc ggctgacgag ctggacgcgg gtcagggcgc
      241 ggaggtcgcg gagcgggtcg ccgcggaccg cgatcagggc ggcccgggcg ccgggcgcca
      301 cccgtccggc acccgccagc ccgagatggt ccgcggcacc ggcggtggca gccaccagcg
      361 cctccccggg gctcatgccc gcctcgacca tgagccggag ctcctcgacg tcgatcccgg
      421 gcggcggtcc cttgttgccg aggtcggtgc cgtagacgac ccggccgccg gccgccagga
      481 acgtggccag accctggcgc ctggcccggg tgggctcgat gtggagcgtg ggcaccaccg
      541 tcatggagct ggcgagcgcc tcgcacagcg tgacaggcag tggtctggcc gtgaacggcc
      601 agtgggcgag ctcggccacg ccggccgcga gcgccttctc gacctcccgc acgccggcga
      661 cgtgcgcggt cacctcgagc ccgcgcccgc gggcggccgc gaccaccgcg gcgagcgtcg
      721 cctcgggcat cgtggggccg gcgcggccgt ccagcgccac cttcacgacg gcggcgcgcc
      781 cctcggcgag ctccccgacc gcccggaccg cctcggccgg cccggccacc ggccgggccg
      841 tgccaggtgg cgcccaggcg gcacgggtcg ggtagccgcc aggggcggtg aggatctgtc
      901 cggctcggag caggtggggc gacacgggca ccggcgcggc cgcttcgcgc tccaggacgg
      961 ccagccgctc ggctggccag ccgaggtcgc gcacggtggt gacgccgccc aggaggacgg
     1021 cggcgggggc gtggaggtcg agatggacgt gggtgtccac gaagccgggc aggacggtca
     1081 ggccggcggc cgggatcgcg cggatcccct cgccgggcgc cagctcctcc ctcgggccga
     1141 cggcgtggac ccgcccgtcg cgcacgacga ccgtgtggcc ggggagcggc ggtcctcccg
     1201 acccgtcgag caccgtcgct ccgacgagcg cgaaggggac gggggccgct ggcatggccc
     1261 ggagcctacg accggccggg gtggagatgc agccggtacc atccggacga ggccggtgcg
     1321 caagttggga ggttggcgtg acagcggcag acggggagct ccggttcgac ccgatcgtgc
     1381 tcgacggccc acccgacggc gccgtcccgc tcgagctggg ccggcgggtg gcgctgtacg
     1441 tctgcggcat cacgccctat gacaccgccc acctggggca cgcgttcacc tacgtggcgt
     1501 tcgacaccct ggtgcgcttc ctgcgccggc gtgggcacga ggtcgactac tgccagaacg
     1561 tcaccgacgt cgacgacgac gtcctcgccc gggcggcgcg caacggcgag gactacctgg
     1621 ccctcggccg gcgcgagacg gcggcgttcc tcgaggtcat ggacgcgctg aacgtcgccc
     1681 ggccgacgtg gtcccccagg gcgaccgagg agatcggtcc catggtcgat ctggcggcgc
     1741 ggctcgtcgg ggagggccac gcctacgagg tcgacggcac cgtcttctt
//
LOCUS       DASPYY010000126         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_583705, whole genome shotgun sequence.
ACCESSION   DASPYY010000126 DASPYY010000000
VERSION     DASPYY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583705"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VEP73_01890"
     CDS             <1..705
                     /locus_tag="VEP73_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYT25224.1"
                     /translation="VSLGGDGTMLRAMRLTVGCRAPVLGVNLGRLGFLAEIDIPELSG
                     ALSAIEDHRFTTEPRSGVGATLDGHDVVAFNDIALVRVPGRGTPAVGLRVEGHSFVRY
                     AADAVIVATPTGSTAYNFSAGGPIVSPSAEGLLVTPAAPHSAFNRALFLSGGEQLALE
                     VLPTSGELAVEADGAVVGYLGPGDVIDLTLLPAAAKVVRLGRTTFYQRAQRRLRLTGS
                     AEIDGDDPPEAPPGAP"
     gene            complement(712..1683)
                     /locus_tag="VEP73_01895"
     CDS             complement(712..1683)
                     /locus_tag="VEP73_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYT25225.1"
                     /translation="MRAVVVERHGGPEVLEVRDRAAPHAGPGQLVVDVAVAGVNFMDI
                     YQRQGGASYAGDLPYVPGAEGAGTVAAAGPGVTGVAGGDRVAWSGVPGSYAEQVVVPA
                     DRAVRLPDGVELQVAAAVMLQGMTAHYLANGTYPVNEGDPVVVHAAAGGVGLLLTQVV
                     KMRGGVVIATTSTADKAELARQAGADHVADYDHFATAVREVTDGAGAAVVYDGVGRAT
                     FDDSLAALRRRGYMVLYGAASGPVPPLDPRRLASGGSLFLTRPTLGDYIAGRDELLRR
                     ADDLFTWIRQGRLTVRIGGTYPLDEAARAHEDLAARRTTGKLLLLPR"
BASE COUNT          236 a          728 c          694 g          250 t
ORIGIN      
        1 gtcagcctcg gcggcgacgg cacgatgctg cgggcgatgc ggctgacggt cgggtgccgc
       61 gcgccggtgc tgggggtgaa cctgggccgc ctcgggttcc tcgccgagat cgacatccct
      121 gagctgtccg gggcgctctc ggccatcgag gaccaccggt tcaccaccga accccgctcg
      181 ggcgtcgggg ccaccctcga cgggcacgac gtcgtggcct tcaacgacat cgccctggtc
      241 cgcgtccccg gacgtggcac ccccgccgtc gggctgcgcg tcgagggcca ctccttcgtg
      301 cgctacgccg ccgacgcggt gatcgtggcc acgccgaccg ggtcgaccgc ctacaacttc
      361 tcggccggcg gcccgatcgt ctccccgagc gcggaagggc tgctcgtcac ccccgccgcc
      421 ccgcactcgg cattcaaccg ggcgctgttc ctgtcgggcg gcgagcagct cgccctggag
      481 gtcctgccca ccagcggcga gctcgcggtc gaagccgacg gcgcggtcgt cggctacctc
      541 gggcctggcg acgtcatcga cctgaccctg ctgcctgccg ccgcgaaggt ggtccggctc
      601 gggcgtacca ccttctacca gcgcgcgcag cgcaggctcc gcctgaccgg ctccgcggag
      661 atcgacggtg acgacccacc cgaagcgccg cccggcgcgc cgtgatgccg ctcagcgggg
      721 caggaggagg agcttgccgg tcgtgcgccg cgcggcgagg tcctcatggg cgcgggcggc
      781 ctcgtcgagc gggtaggtgc cgccgatgcg gaccgtgagc cggccctgcc tgatccaggt
      841 gaacaggtcg tcggcgcggc gcagcaactc gtcacggccg gcgatgtagt cgccgagcgt
      901 cgggcgggtg aggaacagcg acccgccgga ggcgagccgc cgcggatcca ggggaggcac
      961 cggcccgctg gccgcgccgt acaggaccat gtagccacgc cggcgcagcg ccgccaggct
     1021 gtcgtcgaag gtggcccgcc cgacgccgtc gtacacgacc gccgcgcccg ccccgtcggt
     1081 gacctcccgc accgcggtgg cgaagtggtc gtagtcggcg acgtggtcgg cgcccgcctg
     1141 cctggcgagc tcggccttgt cggcggtcga ggtggtggcg atgacgacgc cgccacgcat
     1201 cttgacgacc tgggtgagca ggagcccgac cccgcccgcc gccgcgtgca cgacgacggg
     1261 atcgccctcg ttcacggggt aggtgccgtt ggccaggtag tgggcggtca tgccctgcaa
     1321 catgacggcc gccgcgacct gcagctccac cccgtcgggg aggcggacgg cgcgatcggc
     1381 gggaacgacc acctgttcgg cgtagctgcc cggcaccccg ctccacgcga cgcggtcacc
     1441 cccggcgacg ccggtgacgc ccgggccggc ggccgccacg gtgccggcgc cctcggcgcc
     1501 cgggacgtag ggcaggtcgc cggcgtagga ggcaccgcct tgccgctggt agatgtccat
     1561 gaagttgacg ccggcgaccg ccacgtcgac gacgagctgg ccaggacccg cgtgcggggc
     1621 ggcacggtcg cgcacctcga ggacttccgg cccgccgtgc cgctccacga ccactgcgcg
     1681 catgcgctca tcctccctgc ccgccaggtt ccgcgaccgg atgcgcattc aacaacggcc
     1741 agcccgcgtc cgtcacggta tgatcgccga ccggcacctc ccgacgggtc agggacgcag
     1801 gtcgggttcg acgctcgcac gccctcgccg ccgctcgcac gccctcgccg ccccggtcga
     1861 ggctgtggtt ggaggcggtc gtgcaggtgt cgtagccgtg ccggactt
//
LOCUS       DASPYY010000127         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2269195, whole genome shotgun sequence.
ACCESSION   DASPYY010000127 DASPYY010000000
VERSION     DASPYY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269195"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VEP73_01900"
     CDS             <1..388
                     /locus_tag="VEP73_01900"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYT25226.1"
                     /translation="YEVPGALATITAHRDLEDLTLDRDVLHEKARLEVRYAELVYDGL
                     WFSPLKAALDAFVAETQRSVTGEVRLEFSPGRCQVTGRRAERSLYRPDLATYEGGDAF
                     DHDAGRGFVALWGLPARTWARVNPRG"
     gene            381..>1823
                     /gene="argH"
                     /locus_tag="VEP73_01905"
     CDS             381..>1823
                     /gene="argH"
                     /locus_tag="VEP73_01905"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYT25227.1"
                     /translation="MDSEPARPGSPPAGEPGAAAPAPGGPLWAGGFAAPPHPDMWAYT
                     LSLGVDRRLWREDVAGSRAHVRGLVRAGVLDEGEGKLLLDGLASVAAEFAAGTFAWAP
                     ADEDVHTAVERRLTELVGPVGGKLHTGRSRNDQVATDLHLWIKGAVASAVEAVAGLID
                     ALVGAAVAAPAAVVPGLTHLQPAQPVLWGFQLAAHGFALARDAGRFQDAGRRADYSPL
                     GAGAVAGSSFPLDPVATAIDLGFGAAFGNAMDATSARDAVAELLAACAIAMVHASRLA
                     EDVVLWAGRGWVLLPDAWATGSSMLPQKRNPDVAELARGRAGTVIGRLAGFLATLKGL
                     PLAYARDLQEDKAVALEAVDTVVGSMGALAGLVAGLEPVAARMRAAAEEADATAVDLA
                     EALVRAGVPFREAHAAVGAAVRAAAARGLRLADLPAEDLHAVHPALAGADPAVLSVDG
                     SLAAKAVPGSTAPSRVAEQLTVLSVAAANAR"
BASE COUNT          184 a          715 c          709 g          215 t
ORIGIN      
        1 ctacgaggtc cccggcgccc tcgcgacgat caccgcccac cgcgacctcg aggacctgac
       61 cctggaccgg gacgtgctgc acgagaaggc gcggctcgag gtccgctacg ccgagctggt
      121 ctacgacggg ctgtggttct cgccgctcaa ggccgccctc gacgcgttcg tggccgagac
      181 gcagcgaagc gtcaccggtg aggtgcgcct cgagttctcg cccggccgct gccaggtcac
      241 cgggcgccgg gccgagcggt cgctgtaccg gcccgacctg gccacctacg agggcggcga
      301 cgccttcgac cacgacgccg ggcgcgggtt cgtcgccctg tggggcctgc ccgcccggac
      361 ctgggcgcgg gtcaaccccc gtggatagcg agccagcgcg gcccggctcc ccgccggcgg
      421 gggagccggg cgcggccgcg ccggccccag gggggccgtt gtgggcgggc ggcttcgccg
      481 ccccgcccca ccccgacatg tgggcctaca cgctgtccct cggcgtggac cggcggctgt
      541 ggcgggagga cgtcgccggc agccgcgccc acgtccgggg gctcgtgcgg gcaggggtgc
      601 tcgacgaggg cgagggcaag ctgctgctcg acggcctggc ctcggtcgcg gccgagttcg
      661 ccgcggggac gttcgcctgg gcgcccgccg acgaggacgt ccacaccgcg gtcgagcgcc
      721 ggctcaccga gctcgtcggg ccggtcggcg gcaagctgca caccggccgc agccgcaatg
      781 accaggtcgc caccgacctg cacctgtgga tcaagggcgc cgtggcgagc gccgtggagg
      841 cggtcgccgg cctgatcgac gcgctggtgg gggcggccgt ggcggctccg gcggcggtgg
      901 tccccgggct cacccacctg cagccggcgc agcccgtgct gtggggcttc cagctcgccg
      961 cgcacgggtt cgcgctcgcc cgcgacgccg ggcgcttcca ggacgccggg cgccgggccg
     1021 actacagccc gctcggggcc ggcgccgtgg cggggtcgtc gttcccgctc gacccggtcg
     1081 cgacagccat cgacctcggc ttcggcgccg cgttcggcaa cgcgatggac gccacctccg
     1141 cccgggacgc cgtcgccgag ctgctggccg cgtgcgcgat cgcgatggtg cacgcgtccc
     1201 ggctggccga agacgtcgtc ctgtgggccg gccggggctg ggtcctgctg cccgacgcct
     1261 gggcgaccgg ctcgagcatg ctgccccaga agcgcaaccc cgacgtcgcc gagctggcca
     1321 gggggcgggc aggcaccgtg atcggccggc tcgcgggctt cctcgccacc ctcaagggcc
     1381 tgccgctcgc ctacgcccgc gacctgcagg aggacaaggc ggtcgcgctg gaggccgtcg
     1441 acaccgtcgt cgggtcgatg ggggccctgg ccgggctcgt cgccggcctc gagccggtgg
     1501 cggcccgcat gcgcgcggcg gccgaggagg ccgacgccac cgccgtcgac ctcgccgagg
     1561 cgctcgtgcg ggcgggggtg ccgttccgcg aggcgcacgc ggccgtgggc gccgccgtcc
     1621 gggcggccgc ggctcgcggc ctgcgcctgg ccgacctgcc cgccgaggac ctccacgccg
     1681 tccatccggc cctggccggc gccgacccgg ccgtgctctc ggtcgacggc agcctggcgg
     1741 ccaaggcggt cccgggctcg acggcgccgt cccgggtcgc cgagcagctc accgtgctct
     1801 cggtggcggc cgccaacgcc cgc
//
LOCUS       DASPYY010000128         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2549845, whole genome shotgun sequence.
ACCESSION   DASPYY010000128 DASPYY010000000
VERSION     DASPYY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549845"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VEP73_01910"
     CDS             <1..885
                     /locus_tag="VEP73_01910"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HYT25228.1"
                     /translation="HLHDRAGKVLSYHDEYAYLDPGNVGRASYQAQCLRRLARVRADG
                     YDGILGDDANVYPGHGLGAAGGTPLAEYPADAAYGDAMVAAMGRIGPAAERLGLLVVA
                     NVGADPGTPAHRGRALAIARASSGLFQEFWTRWKGAVPGQGGAAWEAGATLAEQVEAL
                     GKRFVASTYGGPGTGGAAADQQYAVASFWLVWDGRHDSGWGYNVLGDPAAGFSPAWGP
                     DIGTPTDAARVRVGTGWRRRYSAGVALVNPAPSASQRFDLGAGYRRPDGSVATTVTLP
                     PMSGMVLTRTETTPGDRS"
     gene            882..>1548
                     /locus_tag="VEP73_01915"
     CDS             882..>1548
                     /locus_tag="VEP73_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYT25229.1"
                     /translation="MSLGRRAVRGTLWVGASSWINRLVLLVVLAMLARRLTPREFGVL
                     SVLGLARNSLVILGGYGFGDAVVYQRDRIGQAARTAFTATTVLGVLLGLALLPAAPAV
                     AAFFRVPEATGPIRAYALVIAVGTASRVPLAMLTRELAFGRRFVPEAVGSVVGGLVTI
                     GAVLGGRGVWSVVVGDVVREVLVFALVLAVLPERFGFGWDRGLALAMWRYARHALTSE
                     VFEF"
BASE COUNT          159 a          573 c          601 g          215 t
ORIGIN      
        1 cacctgcacg accgcgccgg gaaggtgctg tcgtaccacg acgagtacgc ctacctcgac
       61 cctgggaacg tcgggcgcgc ctcctaccag gcgcagtgcc tgcgccgcct cgcgcgggtc
      121 agggccgacg gctacgacgg catcctcggc gacgacgcca acgtctaccc cgggcacggg
      181 ctgggtgcgg ccggcggcac gcccctggcc gagtaccctg ccgacgccgc ctacggcgac
      241 gcgatggtcg cggcgatggg ccgcatcggc ccggccgcgg agcggctcgg cctgctcgtc
      301 gtcgcgaacg tgggcgccga cccggggacg ccggcgcacc gcggcagggc gctggcgatc
      361 gcgcgcgcga gctccggcct cttccaggag ttctggacgc gctggaaggg tgcggtgccc
      421 ggccagggag gcgcggcgtg ggaggcgggc gccacgctcg cggagcaggt ggaggcgctc
      481 ggcaagcgct tcgtcgccag cacctacggc gggccgggca cgggcggcgc agccgccgac
      541 cagcagtacg cggtcgcgag cttctggctg gtgtgggacg gccggcacga cagcggctgg
      601 gggtacaacg tcctgggcga tcccgcggcg gggttctcgc cggcctgggg cccggacatc
      661 ggaacgccca ccgacgccgc gcgggtgcgg gtcggcacgg gctggcggcg ccgctacagc
      721 gccggggtcg ccctcgtgaa cccggcgccg tcggcgtcgc agcgcttcga cctcggcgcc
      781 ggctaccgca ggccggacgg gtcggtcgcg acgaccgtga cccttccgcc catgagcggc
      841 atggtgctca ccaggacgga gacgacgccc ggggaccgct cttgagcctc ggccggcggg
      901 ccgtgcgcgg cacgctctgg gtcggggcga gctcatggat caaccggctc gtcctgctgg
      961 tcgtcctcgc catgctggcg cggcggctca ccccgcgcga gttcggcgtc ctgagcgtcc
     1021 tcggcctcgc ccgcaactcc ctggtcatcc tcggcggcta cgggttcggc gacgccgtcg
     1081 tctaccagcg tgaccggatc gggcaggcgg ccaggaccgc cttcacggcc acgaccgtcc
     1141 tcggcgtgct gctggggctc gccctgctgc ccgccgcccc cgcggtggcg gcgttcttcc
     1201 gcgtcccgga ggccaccggc cccatccgcg cctacgcgct cgtcatcgcc gtgggcacgg
     1261 cctcgcgggt cccgctggcg atgctcaccc gcgagctggc gttcggcagg cgcttcgtgc
     1321 ccgaggcggt cggcagcgtc gtgggggggc tggtcaccat cggcgcggtg ctcggcggca
     1381 ggggcgtgtg gtccgtggtc gtcggcgacg tcgtgcgcga ggtgctggtc ttcgccctgg
     1441 tgctcgccgt cctccccgag cggttcgggt tcggctggga ccgcggcctc gcgctggcga
     1501 tgtggcgcta cgcacggcac gcgctgacct cggaggtgtt cgagttct
//
LOCUS       DASPYY010000129         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_584227, whole genome shotgun sequence.
ACCESSION   DASPYY010000129 DASPYY010000000
VERSION     DASPYY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584227"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..890
                     /locus_tag="VEP73_01920"
     CDS             285..890
                     /locus_tag="VEP73_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT25230.1"
                     /translation="MEGRPLDTDDRLVERAREGDVHAYGVLVLRYQDLAYRTARLVTG
                     AAADAEDAAQEGFVKAYYALPRFRPGAAFRPWLLAIVANEARNRRRTARRQGDLAVRV
                     AEDRPSGDAAPSPEAAALAGERRRVLLAAVNRLPEADRQVIACRYFLELSEAEVAAAL
                     GCRRGTVKSRTSRALARLRAVLAEHGDGHVFPMTTLEGGGE"
     gene            887..1726
                     /locus_tag="VEP73_01925"
     CDS             887..1726
                     /locus_tag="VEP73_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25231.1"
                     /translation="MTGRELERALADLGAHLEHPATPPVAEAVAARLAGAPAPADGRG
                     RRPPARPRWRRLAVACLVIVLLAIAVLAASPATREAAARLFGLRGVRIQLGGPAGTAP
                     PTSPRTGPATTARTGPGAELHLGTPASLAQARALVRFPVAVPTAPGFEHPDAVYVDPG
                     QPADGRVDLVYLGRPGLPASPDTGVGLLVTQFRAGISEEFVKKVQMSEGGIEAVTVHG
                     GRGYWFAGRHDFAFVDRHGNGGYEVSRVAGSTLVWERGDVTLRLEGQVSKQEALRIAE
                     SMR"
     gene            complement(1778..2659)
                     /locus_tag="VEP73_01930"
     CDS             complement(1778..2659)
                     /locus_tag="VEP73_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25232.1"
                     /translation="MTAPTPATMGGADAPPVVEDPTAPSMRAEALAGPRVPSGAETPA
                     GPAQRAPARPAIPTRPGATEGAGAAGGTGAAGGATGGAEMPPVPRVDAWELGPRPPAR
                     LGEQRGRRGGAEHGAAPGGSEPGAAPGGTAGAGGYVRLRLRLDAGRLSLAGARAVEGP
                     LLQPVRLHAGLACEVTVAGRRVAVDSIPDLGVWRSLPAPGHPGGGERKGHHVAELDAF
                     EFQVRVPRGDLPIGSLPDVRVAVYRCKGGAPSGEIGPGSIAEQFRRELREVASLDGVR
                     LWELPADVQEELRDQLR"
     gene            complement(2673..>2967)
                     /locus_tag="VEP73_01935"
     CDS             complement(2673..>2967)
                     /locus_tag="VEP73_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25233.1"
                     /translation="RADLFVYNHEDWGREYLGAMVSNSKELNATRREDWVGEWSLGAD
                     DRFVPCNYQGGAGVPDLIVHDGDWLGMIRATPALVLDRVYHRWVHNYRYGRNW"
BASE COUNT          315 a         1154 c         1160 g          338 t
ORIGIN      
        1 cggtcgggcc gccggctgct gccgctggcc accagaagga cgctgaaggc ggtctcctgg
       61 cggttcgccg acgccgtcga gcgcggcgac ttcgagcagg ccgaggccga ggtggcacag
      121 ctcttcaaac ggtcggcctg agcccggccc ggaccgcccg cgggaccgcg gcccgggccg
      181 ccccttgcgc gcggcggccg cgtaggggag cgttcgggct gcccgccgtt ccccggctgg
      241 aaccgaacgc ccggtgtcgg tgtaccgact gtgacatcga gtccgtggag ggccgaccac
      301 tggacaccga cgaccggctc gtcgagcgcg cgcgggaagg ggacgtccac gcctacggcg
      361 tcctggtgct gcggtaccag gacctcgcct accgcacggc ccggctggtc accggggcgg
      421 ccgccgacgc cgaggacgcc gcccaggagg ggttcgtgaa ggcgtactac gcgctccccc
      481 ggttccggcc cggcgcggcg ttccgcccct ggctgctggc catcgtcgcc aacgaggccc
      541 gcaaccggcg gcggacggcc cggcgccagg gcgatctggc ggtccgggtc gccgaggacc
      601 gcccctcggg ggacgcggcc ccgtcccccg aggcggcggc gctcgccggc gagcggcgtc
      661 gcgtcctgct cgccgccgtg aaccgcctgc ccgaggccga ccggcaggtg atcgcctgcc
      721 gctacttcct cgagctgtcc gaggccgagg tggcggcggc gctcggctgc cgcaggggca
      781 cggtcaagtc gcgcacctcg cgcgcgctcg cgcgcctccg ggcggtgctg gccgagcacg
      841 gcgacgggca cgtgttcccg atgacgacgc tcgagggggg aggcgaatga ccggccgcga
      901 gctcgagcgc gccctggccg acctgggcgc ccacctcgag cacccggcca ccccgccggt
      961 cgccgaggcg gtcgccgccc ggctcgccgg ggcgccggcg cccgcggacg gccggggccg
     1021 gcggccgccc gcccggccgc gttggcggcg cctggccgtc gcctgcctgg tcatcgtgct
     1081 cctggcaatc gccgtcctgg ccgcctcgcc ggccacccgc gaggcggccg cccgcctgtt
     1141 cggcctgcgg ggcgtcagga tccagctcgg cgggccggcc ggcaccgccc cgccgacgtc
     1201 cccgcggacc gggccggcga cgaccgcgcg gaccgggccg ggggccgagc tgcacctggg
     1261 cacgccggcg agcctggccc aggccagggc gttggtacgg tttccggtcg ccgtgccgac
     1321 cgcccccggc ttcgagcacc cggacgcggt gtacgtcgac cccggccagc cggcggacgg
     1381 gcgggtcgat ctcgtctacc tgggacgccc ggggctgccc gcctccccgg acaccggtgt
     1441 cggcctgctc gtcacccagt tccgggcggg gatcagcgag gagttcgtca agaaggtcca
     1501 gatgtccgaa ggcggcatcg aggcggtcac cgtgcacggc gggcgtggct actggttcgc
     1561 cggccggcac gacttcgcct tcgtcgaccg gcacggcaac ggggggtacg aggtcagccg
     1621 cgtggcaggg agcacgctgg tctgggagcg cggcgacgtc accctgcgcc tcgagggcca
     1681 ggtgtccaag caggaggcgc tgcgcatcgc cgagtcgatg cgctgacgtc aggcaccggc
     1741 tctcccggtc cgaggccagg ctccgcggcg ccgtcggcta gcggagctgg tcgcgcagct
     1801 cctcctgcac gtccgcgggc agctcccaga gccggacgcc gtcgaggctg gcgacctccc
     1861 ggagctcgcg gcggaactgc tcggcgatcg agccggggcc gatctccccg ctcggggcgc
     1921 cccccttgca gcggtagacg gccacgcgga cgtccggcag ggacccgatc ggcaggtcgc
     1981 cgcgcgggac ccggacctgg aactcgaagg cgtcgagctc ggccacgtgg tggcctttgc
     2041 gctcgccgcc acccgggtgg ccgggcgcgg gcagggagcg ccagaccccg aggtcgggga
     2101 tggagtcgac cgccacccgg cggccggcca cggtcacctc gcaggccagg ccggcgtgga
     2161 gccgcaccgg ctggaggagc gggccctcga ccgcgcgggc gccggccagc gacaggcggc
     2221 cggcgtccag gcgcagccgc agccgcacgt acccgcccgc gccggccgtg ccgccaggtg
     2281 cggcccccgg ctcgctgccg ccaggtgcgg ccccgtgctc cgcgccgccg cgccggccgc
     2341 gttgctcgcc gagccgggcc ggcggccggg ggccgagctc ccaggcgtcc acccgcggga
     2401 cgggcggcat ctcggccccg cccgtggccc cgcccgcggc gccggtcccg cccgcggcgc
     2461 cggcaccctc cgtggccccg gggcgggtgg ggatcgcggg ccgggcgggc gcccgctggg
     2521 cggggccggc cggcgtctcg gcgccgctcg ggacccgcgg cccggcgagc gcctcggcgc
     2581 gcatcgacgg tgcggtcggg tcctcgacga ccgggggcgc gtccgcgccg cccatcgtgg
     2641 ccggggtcgg agcggtcatg gctcgggcct cctcaccagt tgcggccgta ccggtagttg
     2701 tggacccagc ggtggtagac gcggtcgagc acgagcgccg gggtcgcccg gatcatgccg
     2761 agccagtcgc cgtcgtggac gatcaggtcc gggacgccgg cgcctccctg gtagttgcag
     2821 ggcacgaagc ggtcgtcggc accgaggctc cactccccca cccagtcctc gcggcgggtc
     2881 gcgttcagct ccttggagtt ggagaccatc gcaccgaggt actcgcggcc ccagtcctcg
     2941 tggttgtaga cgaacaggtc ggcccgg
//
LOCUS       DASPYY010000130         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1427262, whole genome shotgun sequence.
ACCESSION   DASPYY010000130 DASPYY010000000
VERSION     DASPYY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427262"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /gene="pgi"
                     /locus_tag="VEP73_01940"
     CDS             <1..457
                     /gene="pgi"
                     /locus_tag="VEP73_01940"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646682.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYT25234.1"
                     /translation="PCDFIGFCQPNHQLGDHQDLLMANLFAQTEALAFGKTPEEVAAE
                     GVPAFQVPHRVFPGNRPTNTILATKLTPSTLGQLVALYEHKVFTQGTIWNINSFDQWG
                     VELGKVLAQRIAPQLQSKDEPALDHDSSTNTLIRRYRKRSMNTLASPSP"
     gene            complement(509..1321)
                     /locus_tag="VEP73_01945"
     CDS             complement(509..1321)
                     /locus_tag="VEP73_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT25235.1"
                     /translation="MTTTGNDDLSPTDPRRDLRGAGALVTGGGSGLGAATARELARRG
                     ARVAIVDRDEEAAAGVAKSLGESGRAFSADVTDEAQLAAAVDGAVAAFGGLRIAVLCA
                     GVGVAQRVLGRDGPASLELFETVLRINLTGTYNALRLVAAAMASNDPDGDNQRGVVIM
                     TASVAAFDGQIGQTAYSASKGGVAALTLPAARDLSRNGIRVCTIAPGVFDTPMLAGLP
                     EAARESLAADIPFPHRLGTPEEYALLACQIAEHPYLNGEVIRLDAALRMAPK"
     gene            complement(1360..>2228)
                     /gene="lhgO"
                     /locus_tag="VEP73_01950"
     CDS             complement(1360..>2228)
                     /gene="lhgO"
                     /locus_tag="VEP73_01950"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HYT25236.1"
                     /translation="RLEAAEIREREPHLRALAGIEVPTTGVVDYRQVAVALVRLLQEA
                     GVALRLGTEVRGFATGSGPNGREHVLQTTAGDGELRAGYLVTCAGLHADRLARAAGAD
                     PGTRIMPFRGEYYELSPRARDLVRGLVYPVPDPAFPFLGVHLTRTLDGNVHVGPNAVP
                     AASREGYTWSSVSPRDLAATVAYPGWWRLARRYWRTGLQEITRSLSMRAFLADLRRLY
                     PEVSAADLLPAPAGVRAQALSRDGRLVDDFVIAHANRATHVCNAPSPAATASLEIARE
                     IVAGLPDDAASG"
BASE COUNT          312 a          804 c          824 g          288 t
ORIGIN      
        1 cccctgcgac ttcatcggct tctgccagcc caaccaccag ctcggcgacc accaagacct
       61 cctcatggcc aacctcttcg cccagaccga agccctcgcc ttcggcaaga cccccgagga
      121 ggtcgccgcc gagggcgtcc ccgccttcca ggtcccccac cgggtgtttc ccggcaaccg
      181 ccccaccaac acgatcctgg ccaccaagct caccccgagc accctcggcc agctcgtcgc
      241 cctctacgaa cacaaggtct tcacccaggg aaccatctgg aacatcaact ccttcgacca
      301 gtggggcgtc gaactcggca aggtcctcgc ccagcgcatc gccccccagc tccagtccaa
      361 ggacgagccg gccctggacc acgacagctc caccaacacg ctgatccgcc gctaccgcaa
      421 gcgctcaatg aacacgcttg cgtcgccctc gccatgaccc tgctgtgctg gcgggaccgt
      481 ctcactccca ctccccacag cgctcatttc acttcggggc catgcggagg gcggcgtcga
      541 ggcggatgac ctcgccgttc aggtaagggt gctcggcgat ctggcaggcg agcagggcgt
      601 attcctcagg cgtgccgaga cggtgcggga aagggatgtc agcggcgagg gactcgcggg
      661 cagcctcggg gagaccggcg agcatggggg tgtcgaacac acctggcgcg atcgtgcaga
      721 cccggatgcc gttgcgggac aggtcgcggg cggcggggag ggtgagcgcg gcgacgccgc
      781 ccttggaggc ggagtaggcg gtctggccga tctggccgtc gaaggcggcg accgaggcgg
      841 tcatgatcac cacgccgcgc tgattgtcgc cgtcggggtc gttgctggcc atggcggcgg
      901 cgaccaggcg cagggcgttg taggtgccgg tcaggttgat gcgcagcacg gtctcgaaca
      961 gctccaggct ggcggggccg tcacggccga ggacccgctg ggcgacgccg accccggcgc
     1021 acaacaccgc gatccgcagg ccgccgaacg ccgccaccgc gccgtcgacc gcggcggcga
     1081 gctgggcctc gtcggtcacg tcggcgctga acgccctgcc gctctccccc agcgacttgg
     1141 cgacgccagc ggccgcttcc tcgtcccggt cgacgatcgc tacccgggcg ccacggcggg
     1201 ccagctcccg ggcggtggcc gcgccgaggc cggagccacc gccggtcacc agcgctccgg
     1261 cgccgcgaag gtccctgcgg gggtcggtgg ggctgaggtc gtcgttgccc gtcgtagtca
     1321 cggcgtctgc ctccttctgc gaagggtcgg tgggtccgct cagccgctgg ccgcgtcgtc
     1381 ggggaggccg gccacgatct cgcgggcgat ctccagggag gcggtcgcgg ctggggaggg
     1441 ggcgttgcag acgtgggtgg cgcggttggc gtgggcgatg acgaagtcgt cgaccaaccg
     1501 gccgtcgcgg gacagcgcct gggcgcggac gcccgccggg gctgggagca ggtcggcggc
     1561 gctcacctcc gggtacaggc gccgcaggtc ggcgaggaac gcgcgcatcg acagcgagcg
     1621 ggtgatctcc tgcaggccgg tgcgccagta gcgacgagcc aggcgccacc agccggggta
     1681 ggcgacagtg gcggcgaggt cgcgggggct caccgacgac caggtgtagc cctcgcggct
     1741 ggcggcgggg accgcgttcg ggccgacgtg gacgttgccg tccagggtgc gggtcaggtg
     1801 caccccgagg aacgggaagg ccgggtcggg caccgggtag accaggccgc ggacgaggtc
     1861 ccgtgcccgg ggggacagct cgtagtactc gccgcggaac ggcatgatcc gcgtgccggg
     1921 gtcggcgccg gcggcgcggg cgagccggtc ggcgtgcagg cccgcgcagg tgaccaggta
     1981 gccggcccgc agctcgccgt ctcccgcagt cgtctgcagg acgtgctcgc ggccgttcgg
     2041 cccgctgccg gtggcgaacc cgcggacctc cgtgcccagg cgcagggcga caccggcttc
     2101 ctggagcagg cggaccagcg cgaccgcgac ctgccggtag tcgaccacgc cggtggtcgg
     2161 cacctcgatc ccggcgaggg cgcgcaggtg cggttcgcgc tcgcggatct ccgccgcctc
     2221 caggcgcc
//
LOCUS       DASPYY010000131         5586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_302439, whole genome shotgun sequence.
ACCESSION   DASPYY010000131 DASPYY010000000
VERSION     DASPYY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302439"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VEP73_01955"
     CDS             complement(<1..626)
                     /locus_tag="VEP73_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT25237.1"
                     /translation="MRPASLLRLCALAVLWGSNFLFIKVAVERLSPAEVVLGRMALGA
                     VVLLAVVVARRVALPRRAGMWAHLAFMALLANLVPYFLFGWGEQRITSGLAGVLNATT
                     PLFTLVIVALSGSGERLTVSRVGGLALGFAGVLVLVAPWRAGSLGGELPGQVACLVAA
                     ACYAVSFVWTRRFLTGRGYPTLALAGAQLGASVVLLGLASPLVLRDPVS"
     gene            complement(711..1280)
                     /locus_tag="VEP73_01960"
     CDS             complement(711..1280)
                     /locus_tag="VEP73_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25238.1"
                     /translation="MKFLDTLLGRSKPKQANLDALFALPGAAITLEAGAGLRPSGQAA
                     VCFKPASSQDFATTSAELTELLSMAVKEAGSTLRVTDDQFGYRWVVIGDPELEDLVTS
                     VHLVNSTLEEHGFGPQLLCSVFGFGSGDQRCHLVYLYKRGTFYPFAPRAGERRDNELE
                     LRVRATLGTDLPVEPDLTRWFALWGIPVA"
     gene            complement(1277..2209)
                     /gene="htpX"
                     /locus_tag="VEP73_01965"
     CDS             complement(1277..2209)
                     /gene="htpX"
                     /locus_tag="VEP73_01965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HYT25239.1"
                     /translation="MARTRFPPDFGLSSRMLLTMFLLGLVYVAFMAVLIALKIPVALV
                     VVIAVGFLLFQYFFSDRIALFAMRGREVTPQQAPELHELIDRLCALADMPKPRVAIAQ
                     SDIPNAFATGRNPKVAVVCVTTGIMRKLDKPELEAVLSHELSHVAHRDVAVMTIASFL
                     GLLAGFVVRMAFYGGVYGGGFGGYGRRDSNNNDSTGAVILLVVLASAVVYALSFLLTR
                     ALSRYRELSADRAGAMLIGRPSLLASALVKVSGDMARIPTRDLRAAEPLNAFFFAPAI
                     APGFSLSSLFSTHPSLEQRLERLSRLEAELGRRA"
     gene            complement(2224..2502)
                     /locus_tag="VEP73_01970"
     CDS             complement(2224..2502)
                     /locus_tag="VEP73_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25240.1"
                     /translation="MIVRIMGEGQFDVPSGHLDGLNRLDGHLMKAVDSGDAAAFTTAL
                     EALLTSVRTAGSELPADYLGPSDLVLPGPDATIDEVREVLGDEGLIPG"
     gene            complement(2499..3275)
                     /locus_tag="VEP73_01975"
     CDS             complement(2499..3275)
                     /locus_tag="VEP73_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HYT25241.1"
                     /translation="MGLMKRFSYIFKAKASRQLDRYEDPRETLDYSYEKQLELLQKMR
                     RGVADVATSRKRIELQAQQLQASAGKLEGQARQAVAQNREDLAREALTRREAIATQLQ
                     DLQGQHDQLKAEEAKLVDASQRLQAKVESFRTRKETIKATYTAAEAQTKIGEAVSGIS
                     EEMGDVGMAMQRAEDKVAQMQARSSAVDELMASGALDDYAASGDRLQAELDRGAASSG
                     VELELTRIKGELGGAPAKQLNASAPAPAPAPAPTQPEDQQ"
     gene            complement(3374..3691)
                     /locus_tag="VEP73_01980"
     CDS             complement(3374..3691)
                     /locus_tag="VEP73_01980"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HYT25242.1"
                     /translation="MTGEDPIHIDLERSGGFAGMSLRASVDTSRLPPDEAGTIAELVD
                     RVDFGALAAGAGAPARAPDRFQYDLVVRRGKERHALSLGESQVTPELRSLLDHLVALA
                     RPR"
     gene            complement(3740..4810)
                     /locus_tag="VEP73_01985"
     CDS             complement(3740..4810)
                     /locus_tag="VEP73_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HYT25243.1"
                     /translation="MTGHHHAIRCILPPDVLERVVLHGDEEERERALRTLATDQSLRS
                     VRAQNAILQAQGPRRLNPHVTEGGTPVRTVLDCGHTQDPARARIVRTEGDPATGDSAA
                     DEAYDGLGATYGLFWNAYERDSIDDNGLQLKGYVHYGDGYDNAFWDGQEMIFGDGDGR
                     LFNRFTISVDVIGHELTHGVTEVEASLVYYRQPGALNESVSDVFGSLVKQHALGQEAD
                     EADWLIGAGLIADAPGQALRSMKAPGTAYDHPLLGKDGQPAHMRDYVQTTADNGGVHT
                     NSGIPNHAFYLAAVNIGGYAWERAGRIWYDALRDQRLQPTAQFRTFARTTLRATQRLG
                     FDTASTEYKAVHEAWAQVGLKL"
     gene            complement(4913..>5586)
                     /locus_tag="VEP73_01990"
     CDS             complement(4913..>5586)
                     /locus_tag="VEP73_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYT25244.1"
                     /translation="NVFPHHEDEIAQSESLVGHQVVATWVHGEHLLAEGRKMSKSAGN
                     FYDLRDVEARGHSPLALRLLFLQARYRTQVNFTWDALAGAERALERLRARVAAWSRDP
                     RGGEPPGRAGYEARFRAAVDDDLDTPRALAVLAEMEGDEGLPPAARWRSALAFDRVLG
                     LDLDRAAEASLPRGAEELIARREAARAGRDWAAADRLRDELAGMGVEVSDTRSGTTWR
                     LVARG"
BASE COUNT          769 a         2004 c         2053 g          760 t
ORIGIN      
        1 gacaccgggt cacgcaggac cagcggcgag gcgaggccga gcagcacgac gctcgcgccg
       61 agctgggcgc cggccagcgc gagcgtcggg tagccccgac cggtcaggaa ccggcgcgtc
      121 cagacgaagg acaccgcgta gcacgcggcc gccaccaggc aggcgacctg ccctggcagc
      181 tccccgccga gcgaccccgc ccgccacggc gcgaccagta cgagcacccc ggcgaagccg
      241 agcgctagcc ctccgacccg gctcacggtg agccgctccc ccgagcccga cagcgccacg
      301 atgaccagcg tgaacagcgg cgtggtggcg ttcagcaccc cggccaggcc ggacgtgatc
      361 cgctgctctc cccagccgaa caggaaatag gggacgaggt tggccagcaa ggccatgaac
      421 gcgaggtgcg cccacatgcc cgcgcgacgc ggcagggcca cacggcgggc gacgaccacg
      481 gcgagcagga ccaccgcccc gagcgccatc cggccgagca ccacctccgc cgggctgagc
      541 cgctccacgg cgaccttgat gaagaggaag ttggatcccc agaggacggc gagggcgcag
      601 aggcgcagga ggctggctgg acgcaccccg ccagccttcc acggatcggc cgcggccgca
      661 cgcgggattc gccgcgggcc ggtggcggcc cggcagccgc cgggcggcgg tcaggcgacg
      721 gggatgcccc agagggcgaa ccagcgggtc aggtcgggct cgaccggcag gtcggtgccc
      781 agcgtggcgc gcacccgcag ctcgagctcg ttgtcgcgcc gctcgccggc gcggggagcg
      841 aacgggtaga acgtcccccg cttgtagagg tagacgaggt ggcaccgctg gtccccgctc
      901 ccgaacccga ataccgagca gagcagctgg ggcccgaagc cgtgctcctc cagggtcgag
      961 ttgaccaggt ggacgctcgt gaccaggtcc tcgagctccg ggtcgccgat gacgacccag
     1021 cggtagccga actggtcgtc ggtgacccgg agggtcgagc cggcctcctt gaccgccatc
     1081 gacaacagct cggtcagctc ggccgaggtg gtggcgaagt cctggctgga ggcgggcttg
     1141 aagcagacgg ccgcctgccc ggacgggcgc aggccggccc cggcctcgag ggtgatcgcg
     1201 gcccctggca gcgcgaacag cgcgtcgagg ttcgcctgct tcggcttgct gcgcccgagg
     1261 agcgtgtcca ggaacttcac gcgcgccgtc cgagctcggc ctcgaggcgg gagagccgct
     1321 cgagccgctg ctcgaggctc gggtgggtcg agaacagcga cgacaggctg aagccggggg
     1381 cgatggcggg ggcgaagaag aaggcgttga gcggctcggc ggcgcgcagg tcgcgggtcg
     1441 ggatgcgggc catgtcgccc gagaccttga cgagcgcgga cgcaagcagc gacggccggc
     1501 cgatgagcat ggccccggcg cggtcggccg acagctccct gtacctcgac agtgcgcgcg
     1561 tgagcaggaa ggacagggcg tacacgaccg ccgaggcgag gacgacgagc aggatcaccg
     1621 cgcccgtgga gtcgttgttg ttcgagtccc gccggccgta gccgccgaac cccccgccgt
     1681 acacgccgcc gtagaaggcc atgcggacga cgaagccggc caggaggccg aggaagctcg
     1741 cgatcgtcat caccgccacg tcgcggtggg cgacgtggga cagctcgtgg gagagcaccg
     1801 cctccagctc gggcttgtcc agcttgcgca tgatcccggt ggtgacgcag accaccgcca
     1861 ccttcgggtt ccggccggtg gcgaacgcgt tcgggatgtc ggactgggcg atcgcaacgc
     1921 ggggcttggg catgtcggcc agcgcgcaca ggcggtcgat gagctcgtgc agctcgggag
     1981 cctgctgagg cgtgacctcc cggccgcgca tcgcgaacag cgcgatcctg tccgagaaga
     2041 agtactggaa gagcaggaac ccaaccgcga tgaccacgac cagcgcgacc gggatcttca
     2101 gggcgatcag aacagccatg aacgccacgt agacgaggcc gagcaggaac atggtgagca
     2161 gcatgcggct cgagaggccg aaatcgggag ggaagcgcgt gcgagccatc gtgatcaacc
     2221 cattcagccg gggatgaggc cttcgtcccc gagcacctcg cggacctcgt cgatggtggc
     2281 gtccggcccg ggcaggacga ggtcggacgg cccgaggtag tcggccggca gctcgctgcc
     2341 cgccgtccgc acgctggtga ggagcgcctc cagggcggtc gtgaacgcag cggcgtcgcc
     2401 gctgtcgacc gccttcatga gatggccgtc gagccggttc agcccgtcca ggtgcccgga
     2461 cgggacgtcg aactgtccct cgcccatgat gcgaacgatc attgctggtc ctcgggctga
     2521 gtcggggcgg gggcgggcgc gggcgccggc gcggaggcgt tgagctgctt cgcgggtgcc
     2581 ccgccgagct cgcccttgat ccgggtgagc tcgagctcga ccccgctgga cgcggcgccg
     2641 cggtccagct cggcctgcag gcggtcgccg ctggcggcgt agtcgtcgag cgcgccggag
     2701 gccatcagct cgtcgaccgc gctcgaccgg gcctgcatct gcgcgacctt gtcctcggcc
     2761 cgctgcatcg ccatgccgac gtcgcccatc tcctcggaga tgccggacac cgcctcgccg
     2821 atcttggtct gggcctcggc ggccgtgtag gtcgccttga tggtctcctt gcgggtgcgg
     2881 aacgactcga ccttggcctg cagccgctgg gaggcgtcca cgagcttggc ctcctcggcc
     2941 ttgagctggt cgtgctggcc ctgcaggtcc tggagctggg tcgcgatcgc ctcgcggcgg
     3001 gtcagcgcct ccctggcgag gtcctcgcgg ttctgcgcga ccgcctgcct cgcctgcccc
     3061 tccagcttgc ccgccgacgc ctggagctgc tgcgcctgca gctcgatccg cttgcgcgac
     3121 gtggccacgt cggccacgcc cctgcgcatc ttctgcagga gctcgagctg cttctcgtac
     3181 gagtagtcca gggtctccct cggatcctcg tacctgtcga gctgcctcga ggccttcgcc
     3241 ttgaagatgt aggagaaccg cttcatcaac cccatcgctc acgctcccgc atggctgccg
     3301 ccagatccct cgtcgccgtc gagtgccagc atacgaccaa cgcgtcccga gcggcctccc
     3361 cgccggtcgc ggctcaccgc ggcctggcca gcgcgaccag gtggtcgagc agggagcgga
     3421 gctccggggt gacctggctc tcgccgagcg agagcgcgtg gcgctccttc ccccgcctga
     3481 ccacgaggtc gtactggaac cggtcgggcg ccctggccgg cgcgcctgcc ccggcggcca
     3541 gggcgccgaa gtcgacccgg tcgaccagct cggcgatcgt gccggcctcg tcgggcggca
     3601 ggcggctggt gtcgacggac gccctgaggc tcatgccggc gaagccgccg ctgcgctcga
     3661 ggtcgatgtg gatcgggtcc tcgccggtca tcgagcctcc cgtgcgcgtg cgccgccccg
     3721 cctgcccgac gggccggcct cagagcttga ggccgacctg ggcccaggcc tcgtggaccg
     3781 ccttgtactc ggtcgacgcg gtgtcgaacc cgagccgctg ggtggcccgc agggtggtcc
     3841 gggcgaacgt gcggaactgg gccgtcggct gcagccgctg gtcgcgcagg gcgtcgtacc
     3901 agatccgtcc cgcgcgctcc caagcgtagc cgccgatgtt gaccgcggcc aggtagaagg
     3961 cgtggttggg gatccccgag ttggtgtgca cgccgccgtt gtcggcggtc gtctgcacgt
     4021 agtcgcgcat gtgggccggc tgcccgtcct tgccgagcag gggatggtcg taggccgtcc
     4081 ccggcgcctt catcgagcgg agcgcctggc cgggcgcgtc ggcgatgagc cccgccccga
     4141 tcagccagtc ggcctcgtcc gcctcctggc cgagcgcgtg ctgcttgacc agcgagccga
     4201 agacgtcgga gaccgactcg ttcagcgccc cgggctggcg gtagtacacc agcgacgcct
     4261 cgacctcggt gacgccgtgg gtgagctcgt ggccgatgac gtcgacggag atcgtgaacc
     4321 ggttgaacag ccggccatcc ccgtcgccga agatcatctc ctggccgtcc cagaaggcgt
     4381 tgtcgtaccc gtcgccatag tggacgtagc ccttgagctg cagtccgttg tcgtcgatgg
     4441 aatcgcgctc gtaggcgttc cagaagagcc cgtaggtggc cccgaggccg tcgtaggcct
     4501 cgtcggccgc gctgtcgccg gtggccgggt ccccctcggt gcggacgatc cgcgcacggg
     4561 ccgggtcctg ggtgtggccg cagtcgagca cggtgcggac cggggtgccc ccctccgtca
     4621 cgtgggggtt gagccgcctg ggcccctgcg cctgcaggat cgcgttctgc gcccggaccg
     4681 agcgcaggga ctggtccgtc gccagcgtcc gcagcgcccg ctcccgctcc tcctcgtcgc
     4741 cgtgcaggac gacgcgctcg aggacgtccg gcggcaggat gcaccggatc gcgtggtgat
     4801 gcccggtcat ggctgtgccc tccctcgctc caacaggtca agcgagaggg tacggtcggg
     4861 tcgcttgagt gtccagggtc ctttgcccgg gccgggtgcc cggggccggg cgtcagccgc
     4921 gggcgacgag gcgccaggtc gtccccgagc gcgtgtccga cacctcgacc cccatcccgg
     4981 ccagctcgtc gcgcaggcgg tcggccgccg cccagtcgcg gccggccctg gccgcctcgc
     5041 gcctcgcgat gagctcctcg gcgcctctgg ggagcgacgc ctcggccgct cggtccaggt
     5101 cgagcccgag cacccggtcg aaggcgagcg cggaccgcca ccgggcggcc ggcgggaggc
     5161 cctcgtcgcc ctccatctcg gccagcacgg cgagggcccg cggggtgtcc aggtcgtcgt
     5221 ccacggcggc gcggaagcgg gcctcgtagc cggcgcggcc cggcggctcg ccgccgcgcg
     5281 ggtcccggga ccaggccgcc acccgcgcgc ggagccgctc gagcgcccgc tccgcgccgg
     5341 ccagggcgtc ccaggtgaag ttgacctgcg tccggtagcg ggcctggagg aacagcaggc
     5401 gcagggccag cggcgagtgc ccccgcgcct cgacgtcgcg caggtcgtag aagttcccgg
     5461 ccgacttcga catcttccgg ccctcggcga gcaggtgctc cccgtggacc caggtggcga
     5521 ccacctggtg gccgaccagc gactccgact gggcgatctc gtcctcgtgg tgggggaaca
     5581 cgttgt
//
LOCUS       DASPYY010000132         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1707833, whole genome shotgun sequence.
ACCESSION   DASPYY010000132 DASPYY010000000
VERSION     DASPYY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707833"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VEP73_01995"
     CDS             complement(<1..269)
                     /locus_tag="VEP73_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYT25245.1"
                     /translation="MPPRPIPPANAPSAPVADRLGRPLRDLRISVTDRCNFRCPYCMP
                     KAVFGPGHPFLPRAELLTFEEITRIVTVAASLGVTKVRLTGGEPLL"
     gene            complement(402..>1635)
                     /locus_tag="VEP73_02000"
     CDS             complement(402..>1635)
                     /locus_tag="VEP73_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25246.1"
                     /translation="VAVAMLYPPAAAGAVAFFGAVQLRGRRPLAAAWWDHSQALVVTA
                     AGSGVFHALASVHDPWRRLLPGYGAAAALMWLAGAAGTVLRTRTPLREALARMNAASP
                     YRFPATFPGMGWFGLPAARLYLAEGLWPVVILLGLLVYARTTCLKAWHQRGELAALLE
                     REQRAAAELRRLDQAKGQFVAVAAHEVRTPLTAILGYVAILRRWPANDPVTTDRYLGI
                     VERQANRLLGLADSLLTAARLEHGGLAAVFRWTSVEEVCKEVVEGLGDDRARVRLDLA
                     PDLPQVSTDGRCLGQVLGNLLDNALKYSPPPDPCELGVRQEADQLVCWVRDQGAGIPR
                     PELDRIFDRFYQVDGSDSRPAAGVGLGLSLVRELVDALGGTISVDSQLGRGSRFTVTL
                     PIRSPTRAEGGDALRRGR"
BASE COUNT          229 a          573 c          649 g          184 t
ORIGIN      
        1 agcagcggct cgccaccggt gaggcggacc ttcgtcaccc cgagcgacgc ggcgacggtg
       61 acgatccggg tgatctcctc gaacgtcagc agctcggcgc ggggcaggaa tgggtgaccg
      121 ggcccgaaga ccgccttggg catgcagtag gggcagcgga agttgcaccg gtcggtcacg
      181 gagatgcgca ggtcgcgcag gggccggccg agccggtcgg cgaccggggc ggacggcgcg
      241 ttggcgggtg ggatcggacg gggcggcatt gctcgacccc tctcacggcg accgtggtgg
      301 cgtggcgacg ttcggtgcgg gcctggactc gcgagggtag cgcaccgcgc gacgcccgga
      361 ccaccggtcc aggcccacgg ccggtcgacc gtgcggccag cctaacgccc gcgccgcagg
      421 gcgtcaccgc cctcagcccg ggtcggggag cggatgggca gcgtgaccgt gaaccggctt
      481 ccccggccga gctggctgtc gacggagatc gtgccgccga gcgcgtcgac cagctcacgg
      541 accaggctga gacccagacc gacgcccgcg gctggtcgcg agtcggaccc gtcgacctgg
      601 tagaagcggt cgaagatgcg gtccagctcc ggtcgaggga tgccggcgcc ctggtcacgg
      661 acccagcaga cgagctggtc ggcctcctgg cgcacgccga gctcgcaagg gtccgggggt
      721 ggcgagtact tgagcgcgtt gtcgagcagg ttgccgagca cctgcccgag gcagcggccg
      781 tcggtggaca cctgcggcag gtccggggcc aggtcgagcc gtaccctggc gcggtcgtcg
      841 ccgaggccct cgacgacctc cttgcagacc tcttccaccg acgtccaccg gaacacggcg
      901 gccagcccgc cgtgctccag cctcgcggcg gtgaggaggc tgtcggccag cccgagcagg
      961 cggttggcct ggcgctcgac gatcccgagg taccggtcgg tggtgacggg gtcattggcg
     1021 ggccagcggc gcaggatggc cacgtagccg aggatggccg tgagcggggt gcggacctcg
     1081 tgcgcggcga cggcgacgaa ctggcccttg gcctggtcca gccggcgcag ctcggccgcg
     1141 gcccgctgct cccgttcgag cagggcggcc agctcgccgc gctggtgcca ggccttcagg
     1201 caggtcgtgc gcgcgtagac gagcagcccg agcaggatca ccaccggcca cagcccctcg
     1261 gccaggtaga ggcgggcggc cgggagcccg aaccagccca tgccgggaaa ggtcgccggg
     1321 aaccggtacg gggacgcggc gttcatccgc gcgagggcct cccggagcgg ggtgcgtgtc
     1381 cgcagcaccg tgccggccgc gcccgcgagc cacatgaggg cggcggcggc gccgtagccc
     1441 gggagcagcc gccgccacgg gtcgtggacg ctcgcaaggg catggaagac gccactgccg
     1501 gcggccgtga cgaccagggc ctggctgtgg tcccaccagg ctgccgcgag cgggcggcgc
     1561 ccgcggagct ggaccgcgcc gaagaacgcg accgccccgg cggccgccgg cgggtacagc
     1621 atggcgaccg cgacc
//
LOCUS       DASPYY010000133         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2410142, whole genome shotgun sequence.
ACCESSION   DASPYY010000133 DASPYY010000000
VERSION     DASPYY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410142"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VEP73_02005"
     CDS             <1..1251
                     /locus_tag="VEP73_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HYT25247.1"
                     /translation="YDRLELERERENLQAEIAELTAILESDQRLRELVSAELAAVAEK
                     FGTPRRTVLLEGSGVQRTAAVPLEVADDPCTVLLSSAGLLARTAVRKAGQLELADEST
                     HGQPDDPATARGRPAKGRGRSTEPRSAHDVIVSAVPSTARGTIGVLTSAGRLIRLGVL
                     ELPALPPTAHSPGLSGGAPVSEFVETQRGETIVAIVAADAAGGGLALGTANGIVKRVA
                     PEYPQNASEFDVIALKEGDRVVGAVQLESEDQDLVFITSDAQLLRFGAASVRPQGRAA
                     GGMAGIRLSPRAAVIWFGAVDPGSVAGNGEPDAQGAAEAPVVVTVAGSARTLPGTAPA
                     SVKATPYAEFPPKGRATGGVRCHRFLKGEDVLVLAWAGPGPARGATAAGTPVQLPPPE
                     GRRDGSGERVREPLAAVGGSAGPS"
     gene            complement(1452..2645)
                     /locus_tag="VEP73_02010"
     CDS             complement(1452..2645)
                     /locus_tag="VEP73_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT25248.1"
                     /translation="MASQPVLYQGGALADGLSPALRTPVTIAVAGGVITGIFDDDDGV
                     AAAGEPAAVVDASGATIVPGFVDCHSHLTLQGGAQWIARGADPTPELIEAAEHNASAL
                     VRAGVRWVRDVGSPRRPDAGDGRERAVALGLRDRWRGRDGYPYIRAAGTWLSKAGTLP
                     AGLAIEVTDADALLAAALGQLDDGADLVKLYLDGPDATAAPWSPDEVRKVVAAVHARG
                     ATVAAHGSGLPNCKLAAEAGVDTLEHGFALDPDIAATMAASGVTLVSTLSVMHSWLTF
                     GTTTLIGRFTDETSKSAITERLEMAEESVRIAYRAGVAIAAGSDFGGGSVRAGHLAWE
                     VEALVKAGLEPWQALGAATWQGGTVLGDPDAGRIKIGGPAHFTLVHGDPLTDPAALWR
                     VWRVV"
BASE COUNT          355 a          989 c         1030 g          366 t
ORIGIN      
        1 tacgaccggc tcgagctcga gcgcgagcgc gagaacctgc aggccgagat cgcggagctg
       61 accgcgatcc tcgagtccga ccagcggctg cgcgagctgg tgtccgccga gctggccgcc
      121 gtcgccgaga agttcggcac gccgcggcgc acggtcctgc tcgagggcag cggcgtccag
      181 cggacggcgg cggtcccgct cgaggtcgcg gacgacccgt gcacggtgct gctatcctcg
      241 gccggcctgc tggctcgcac cgcggtccgc aaggcgggtc agctcgagct cgctgacgaa
      301 agcacccatg gtcaaccgga cgatcctgcg actgcccgcg gccggccggc caagggccgc
      361 ggtcggtcaa cggagccgcg gtcggcgcac gacgtgatcg tctcggccgt accgtcgaca
      421 gctcgcggga ccatcggcgt cctgacgtcc gcgggaaggc tgatcaggct cggtgtcctg
      481 gagctgcccg ccctgccgcc gacggcgcac tcccctggcc tgtcgggcgg ggcgccggtc
      541 agcgagttcg tcgagaccca gcgcggcgag accatcgtgg cgatcgtggc ggccgatgcg
      601 gccggcggag gactcgcgct cggaaccgcc aacggcatcg tcaagcgggt ggcgcccgag
      661 tacccgcaga atgcgtcgga attcgacgtc atcgcgctga aggaaggcga cagggtcgtc
      721 ggtgccgtcc agctcgagag cgaggaccag gacctggtct tcatcaccag cgatgcgcag
      781 ctgctcaggt tcggcgccgc ctcggtgcgc ccccagggca gggccgcggg agggatggcc
      841 gggatcaggc tgtcacctcg cgccgccgtg atctggttcg gcgcggttga tccaggatcg
      901 gtcgccggca acggcgagcc ggatgctcag ggcgcggccg aggcaccggt tgtggtcacg
      961 gtcgcgggca gcgcgcggac gctgccgggt acggcgccgg cgagcgtcaa ggcaaccccc
     1021 tacgcggagt ttccgccgaa gggccgcgct accggcggcg tccgctgcca tcggttcctt
     1081 aagggcgagg acgtgctcgt gctcgcctgg gcgggtccag gcccggctag gggagcgacc
     1141 gcggcaggca cgccggtgca gctgccgccg cccgagggcc gcagggacgg ctcgggcgag
     1201 cgcgtgcgcg agccgctcgc ggcggtcggc ggttccgccg gtccgtccta gcagcccctg
     1261 ggcggcgagc ctgccgcggt ggctccggcg ccatccgcgc ctgcagcaca tcggcaggga
     1321 gcgctcggct ggtattcagc ttcgcggtcc attgtcggta atggcgggcc tcatcgggcc
     1381 cgccaccagc atgccggggg catcgcgccg cgaggaccac gcgtgccggg atctgggtgc
     1441 gcgatcgctg gctagacgac ccgccagacg cgccacaggg cggcggggtc ggtgagcggg
     1501 tcgccgtgca cgagcgtgaa gtgcgcgggg ccgccgatct tgatccggcc agcgtccggg
     1561 tcgccgagta ccgtcccgcc ctgccaggtc gccgcgccga gcgcctgcca cggctcgagt
     1621 cccgccttga ccagcgcctc gacttcccac gcgaggtgac cggcgcgcac cgagccgccg
     1681 ccgaagtcgc tgccggccgc gatagcgacg cctgcccggt aggcgatccg cacgctctcc
     1741 tcggccatct cgagccgctc ggtgatcgcc gacttgctcg tctcgtcggt gaaccggccg
     1801 atcagcgtgg tcgtgccgaa ggtcagccag gagtgcatca ccgagagcgt cgagacgagc
     1861 gtgacaccgc tcgccgccat cgtggcggcg atgtccggat cgagggcgaa cccgtgctcg
     1921 agtgtgtcga cgccagcctc ggccgccagc ttgcagttag gcagcccgga cccgtgtgcc
     1981 gcgacggtcg cgccgcgggc gtgcacggcg gcgacgacct tgcgcacctc gtcgggagac
     2041 caaggcgcgg cggtcgcgtc tggcccgtcc aggtagagct tgaccaggtc ggcgccgtcg
     2101 tcgagctggc cgagcgcggc tgcgagcagc gcgtcggcat cggtgacctc gatcgcgagc
     2161 cccgctggca gggtccctgc cttgctcagc cacgtcccgg cggcgcggat gtaggggtag
     2221 ccgtcgcgcc cgcgccagcg gtccctgagg ccgagcgcga ccgcgcgctc gcggccgtct
     2281 ccggcatcgg gcctgcgcgg ggagccgacg tccctgaccc agcggacccc ggccctgacc
     2341 agcgcgctgg cgttgtgctc ggcagcctcg atcagctcgg gcgtgggatc ggcgccacgg
     2401 gcgatccact gagcgccgcc ctggagcgtc aggtggctat ggcagtccac gaagccggga
     2461 acgatggtcg cgccgctcgc gtccaccacc gcagcaggct cgccagccgc tgccacgccg
     2521 tcgtcgtcgt cgaagatccc ggtgatgacg ccgccggcga ccgcgatcgt gaccggtgtc
     2581 cgcagcgccg ggctgagccc gtccgcgagg gcaccgcctt ggtagaggac cggctggctc
     2641 gccacggcgg tcaggcgtcg atcctggtcg ggtccagctc ggcggcgccg ccgacgatga
     2701 aatcgcgccg cggcgcgacc tcgctgccca tcagcaggtc
//
LOCUS       DASPYY010000134         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_865180, whole genome shotgun sequence.
ACCESSION   DASPYY010000134 DASPYY010000000
VERSION     DASPYY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865180"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEP73_02015"
     CDS             <1..547
                     /locus_tag="VEP73_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25249.1"
                     /translation="ALPDGLPAKAALQAFEKDAAGDLAVFRARVEQWFDDAMSRVSGW
                     YRRRVQLWLVVYAALVCVALNVDTFAIARSLWTNGPVRQAVVAAAGQERGAGLDGVAS
                     GVARVRALDLPITWAGSGTPTSDPRRLPRSAGGWAQKVAGLALSMLALSLGAPFWFDL
                     LGKVTQLRLTGPRPGGEGEAR"
     gene            complement(554..>3338)
                     /locus_tag="VEP73_02020"
     CDS             complement(554..>3338)
                     /locus_tag="VEP73_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25250.1"
                     /translation="GKTCFLDGIKLLVGVTGFASGRTSARYVFDGGPKGAPAERAWLR
                     GTFSNPVVPGGTERLFAPAGRGCEDAERVSVVCLVTADERRYLVRPGQVRWGRPIESD
                     LSDFMDANPAGEWLGPRLYDELLEHTGVTHALRAVLALPQGAIDRIVEERPSGMLRRL
                     LELAGERDAYEALHVHRERWEDARAAYLAAMEQHKAELDRHAALAELAARHREWAEQS
                     DQLAHLRLVAKPAAEYRDLAERIDAQRAQEERLARIVASDQATLGELAEEAPALQATA
                     QGLADEAVSLERQLDEAEGAIRALDVRLASMDAKEAEAEAVAGRMRGLAGGRGVERAE
                     AEATATGAALVAAATRRAAVSAALARCETETAALGEGRLLPPPDVEAFRARLRASGIE
                     ATMVADVLDLSDGGADDAARVRAEAALGDALWALMVPSEAYRDATALAVETGYHGGVV
                     RAGAGEPTGALADVVAPVELGLLLERADASSAHDAKQAHGLASRGKAAVAPDGMRYGD
                     SISRRQAPDQPVIGRLARERRASAVRVEASRLGEELEELDARIPELRTAWLRADRAPE
                     AVHALSGRDGAGTSAGAALREAEALQPSLADRHKQLRRQLREVAERLGAATTELATLR
                     DRKALVEARLARNLPRHAEHRHRAAGLEAELADRRLTPEQRVVVESGALPRTESLLHD
                     IDWLAARAGGDRYGPEVRDARVLARHDAQADALREADRAAAERRRDAEALEARLDESR
                     RRYEERVRSVVRRLSDEFARVCRIAHTDGELRLVTGDRPEEYGVDVLVAHRTGERMRS
                     YRDAAHSGGQRAKIAILLLLATMGSAGAADLLLLDEHIAHLDSTNIDYLAELMHALNH
                     RVQFLLATPTNAEALRLSWCDLQLAFLPRDPGQPYNPPIRILSRLGAGDLTSRLGAGD
                     LTGRFPDGELATP"
BASE COUNT          353 a         1278 c         1282 g          425 t
ORIGIN      
        1 ggcgctcccc gacggcctgc ccgccaaggc ggcgctgcag gcgttcgaga aggacgccgc
       61 cggggacctc gccgtgttcc gtgcgcgcgt cgagcagtgg ttcgacgacg cgatgagccg
      121 ggtgtccggc tggtaccggc gtcgcgtgca gctctggctg gtcgtctacg ccgcactggt
      181 gtgcgtggcg ctgaacgtcg acaccttcgc gatcgcccgg tcgctgtgga cgaacggccc
      241 ggtgcgccag gcggtcgtcg ccgccgccgg ccaggaacgg ggcgcagggc tcgacggcgt
      301 cgcgagcggc gtcgcccggg tgcgggcgct ggacctgccg atcacctggg cggggagcgg
      361 cacgccgacg tcggaccccc gccggctgcc gcggagcgcg ggcggctggg cgcagaaggt
      421 cgccgggctc gccctctcca tgctcgccct ctcgctcggc gcgccgttct ggttcgacct
      481 gctcggcaag gtcacccagc tgcgcctgac gggaccacgg ccgggcgggg agggcgaggc
      541 ccggtagcgg ccctcagggc gtggccagct cgccgtccgg gaagcggccg gtgaggtcgc
      601 ccgcaccgag ccgggaggtg aggtcgccgg caccgagccg ggacaggatg cggatcggcg
      661 ggttgtacgg ctggccgggg tccctcggca ggaaggcgag ctgcaggtcg caccaggaca
      721 gcctgagcgc ctcggcgttg gtcggcgtcg ccagcaggaa ctggacgcgg tggttcagcg
      781 cgtgcatcag ctcggccagg tagtcgatgt tcgtcgagtc caggtgggcg atgtgctcgt
      841 ccaggagcag caggtcggcc gcgcccgccg atcccatcgt cgcgagcagc agcaggatcg
      901 cgatcttggc gcgctgtccc cccgagtggg ctgcgtcccg gtaggagcgc atccgctcgc
      961 cggtgcggtg cgcgaccagc acgtcgacgc catactcctc cgggcggtcg ccggtgacga
     1021 ggcggagctc gccgtcggtg tgcgcgatcc ggcagacgcg ggcgaactcg tcgctcaggc
     1081 ggcggacgac gctgcgcacc cgctcctcgt agcggcgccg ggactcgtcc agcctggcct
     1141 ccagcgcctc cgcgtcgcgg cggcgctcgg cggcggcccg gtcggcctcc cggagggcgt
     1201 cggcctgggc atcgtgcctg gcgaggacgc gggcgtcccg gacctccggg ccgtagcggt
     1261 ccccgccggc ccttgccgcc agccagtcga tgtcgtggag gagggactcg gtgcggggca
     1321 gcgcgccgga ctcgaccacc acccgctgct cgggggtgag gcgccggtcg gccagctcgg
     1381 cctcgagccc ggccgccctg tggcggtgct cggcgtggcg gggcaggttc ctcgccagcc
     1441 tcgcctcgac caacgccttg cggtcccgca gcgtggccag ctcggtggtg gccgcgccga
     1501 gccgctccgc cacctctcgc agctgccgcc tgagctgctt gtggcggtcg gccagcgagg
     1561 gctgcagcgc ctcggcctca cgcagcgccg cgcccgccga ggtcccggcc ccgtcccggc
     1621 ccgagagggc gtgcaccgcc tcgggggcgc ggtcggcccg cagccaggcc gtccgcagct
     1681 cggggatgcg ggcgtcgagc tcctccagct cctcgccgag ccggctcgcc tccacccgca
     1741 ccgccgacgc gcgccgctcc cgggccagcc gcccgatcac cggctggtcg ggcgcctgcc
     1801 ggcgcgagat gctgtccccg tagcgcatcc cgtcgggcgc caccgcggcc ttgccgcggg
     1861 acgccaggcc gtgggcctgc ttggcgtcgt gggcggacga cgcgtcggcc cgctcgagca
     1921 gcaggccgag ctcgacgggg gccacgacgt cggcgagcgc accggtcggc tcgcccgcgc
     1981 cggcccgcac gacgcccccg tggtagccgg tctcgacggc gagcgcggtc gcgtcccggt
     2041 aggcctccga cggcaccatg agcgcccaca gggcgtcgcc cagggcggcc tccgcccgga
     2101 ccctggcggc gtcgtcggca ccgccgtcgc tcaggtcgag gacgtcggcc accatggtcg
     2161 cctcgatgcc cgaggcccgc agacgggcac ggaacgcctc cacgtcgggt ggcgggagca
     2221 gccgcccttc tcccagcgcg gcggtctcgg tctcgcatct ggccagggcg gccgacaccg
     2281 cggcgcgcct ggtcgcggcc gcgacgaggg cggccccggt ggcggtcgcc tccgcctcgg
     2341 cccgctccac gccccggccg cccgccaggc cgcgcatgcg cccggcgacc gcctcggcct
     2401 ccgcctcctt cgcgtccatc gaggccaggc ggacgtcgag cgcccgtatc gccccctccg
     2461 cctcgtcgag ctggcgctcg agcgacaccg cctcgtcggc gagcccctgg gcggtcgcct
     2521 gcagcgcggg cgcctcctcg gcgagctccc cgagcgtcgc ctggtcgctc gcgacgatcc
     2581 tggccagccg ctcctcctgt gcccgctggg cgtcgatccg ctcggcgagg tcgcggtact
     2641 cggcggcggg cttggcgacc aggcggaggt gggcgagctg gtcgctctgc tcggcccact
     2701 cgcgatggcg ggcggccagc tcggcaaggg cggcgtggcg gtccagctcc gccttgtgct
     2761 gctccatcgc ggccaggtac gccgcccgag cgtcctccca ccgttcgcgg tggacgtgca
     2821 gcgcctcgta ggcgtcccgc tcgccggcca gctcgaggag ccgccggagc atgccggagg
     2881 gccgttcctc gacgatccgg tcgatggccc cctggggaag ggcgaggacg gcacgcaggg
     2941 cgtgggtgac gccggtgtgc tccagcagct cgtcgtagag gcggggtccg agccactccc
     3001 cggccgggtt ggcgtccatg aagtcggaga ggtccgactc gatcggccgg ccccagcgca
     3061 cctggccggg ccggaccagg tagcgccgct cgtccgcggt gaccaggcag acgacgctca
     3121 cccgctcggc gtcctcgcag ccgcgcccgg ctggtgcgaa caggcgctcg gtgccgccgg
     3181 ggacgaccgg gttcgagaac gtcccccgca gccacgcccg ctcggcgggc gcccccttcg
     3241 ggccgccgtc gaagacgtag cgggccgagg tccggccgga ggcgaagccg gtgacgccga
     3301 cgaggagctt gatgccgtcc aggaagcagg tcttgccc
//
LOCUS       DASPYY010000135         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_302807, whole genome shotgun sequence.
ACCESSION   DASPYY010000135 DASPYY010000000
VERSION     DASPYY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302807"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..>1687
                     /gene="recN"
                     /locus_tag="VEP73_02025"
     CDS             189..>1687
                     /gene="recN"
                     /locus_tag="VEP73_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYT25251.1"
                     /translation="MLEELRITGLGVIEDTTLPLAGGMNAITGETGAGKTMVVTGIGL
                     LFGGRADAARVRAEPGRAVVEGRLRLAGPVAASVAERVVDAGSEPDEDGSVLLARTVN
                     AEGRSRAHVGGRSVPVSVLGELGEQVVAVHGQSDQLRLLRPAEQRAALDRFAGPEHDK
                     LLAELRERYTQWRAVSDDLDDRRRHARERSQEADLLRLGLDEITRVDPQPGEDDALRA
                     EAQRLEHAEGLRTAADTAHAALAGPGDGVAEAPDATSLLAFARRTLETQSTVDPALGE
                     LAARLTEAATLVGDVAVELGGYLQSLDADPARLQEIYERRAALRALTRKYAEDVDGVI
                     AWAQHAQSRLAELDTSDELLEELERERVRLAGEVAELAGRVRAARRDAAGRFAGAVTV
                     ELTGLAMPHARVDVAVTPRPPGRGEPALTVGGTECGVGPDGADEVELKLVAHPGAPAL
                     PLQKGASGGELSRVMLAIEVVFAGAGGPPTLVFDEVDAGVGGRAAVEIGR"
BASE COUNT          218 a          581 c          694 g          194 t
ORIGIN      
        1 gatcggcgca aggccggaga ctgaccattg tggacaatca tgcgatgtcc gcccaggtca
       61 gcggaaggat ctccgaccgg ctgcgtcagg cagtgcgcgg gtcaagattg tgtctatcct
      121 cctgcgcgga aatccgcgct aatggctgcg cagtagcgac tgtcagaccg ccccgctact
      181 gtcgggtcgt gctggaggag ctacgcatca ccgggctcgg ggtcatcgag gacaccaccc
      241 tcccgctggc cggcgggatg aacgccatca ccggcgagac cggtgcgggc aagacaatgg
      301 tggtcaccgg gatcgggctg ctcttcggcg gccgggccga cgcggcgcgg gtccgggcgg
      361 agccgggccg ggcggtggtc gaagggcggc tgcggctggc cggtccggtg gcggcgagcg
      421 tcgccgagcg ggtcgtcgac gccggttccg agcccgacga ggacggcagc gtgctgctgg
      481 cccgcacggt caacgcggag ggccggtcgc gggcgcacgt gggcggccgg tcggtacccg
      541 tctcggtgct cggcgagctg ggcgagcagg tggtcgccgt gcacggccag tccgaccagc
      601 tgcgcctgct gcggccggcc gagcagcgcg cggcgctgga ccggttcgcc ggacccgagc
      661 acgacaagct gctcgccgag ctgcgcgagc ggtacacgca atggcgggcg gtctccgacg
      721 acctcgacga ccggcgccgg cacgcgcggg aacgcagcca ggaggccgac ctgctgcggc
      781 tggggctgga cgagatcacc cgcgttgatc cgcaaccggg tgaggacgac gccctgcgcg
      841 ccgaggccca gcggctggag cacgccgaag ggctgcgcac cgccgcggac accgcgcacg
      901 cggcgctggc cggccccggc gacggggttg ccgaggcgcc cgacgcgacc agcctgctgg
      961 cgttcgcccg gcggaccctg gagacgcagt ccactgtgga cccggcgctg ggcgagctgg
     1021 cggcgcgcct gaccgaggcg gccacgctgg tcggcgacgt ggccgtcgag ctgggcggct
     1081 acctgcagtc gctggacgcc gacccggccc gcctgcagga gatctacgag cggcgggcgg
     1141 cgctgcgggc gctgacccgc aaatacgccg aggacgtcga cggggtgatc gcctgggcgc
     1201 agcacgcgca gagccgcctc gccgagctgg acacctccga cgagctgctg gaggagctgg
     1261 agcgggaacg ggtccggctg gccggtgagg tcgccgagct ggcggggcgg gtacgcgcgg
     1321 cgcgccggga cgcggccggc cggttcgccg gcgcggtcac cgtggagctg accggcctcg
     1381 ccatgccgca cgcgcgggtc gacgtcgcgg tcaccccgcg cccgcccggc cggggggagc
     1441 cggcgctgac cgtcggcggc accgagtgcg gcgtcggccc ggacggggcc gacgaggtag
     1501 agctcaagct ggtcgcgcac ccgggagcgc cggccctacc gctgcagaag ggcgcgtccg
     1561 gcggcgagct gtcccgggtc atgctcgcga tcgaggtggt gttcgccggc gccggcggac
     1621 cgcccacgct ggtgttcgac gaggtcgacg ccggggtcgg cgggcgggcg gcggtggaga
     1681 tcgggcg
//
LOCUS       DASPYY010000136         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2270688, whole genome shotgun sequence.
ACCESSION   DASPYY010000136 DASPYY010000000
VERSION     DASPYY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270688"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VEP73_02030"
     CDS             complement(<1..396)
                     /locus_tag="VEP73_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYT25252.1"
                     /translation="MKEEGEGGTRAIVAAFLANLGIAVAKFVAFLFTGASSMLAEAIH
                     SVADSGNQVLLLVGGRRSKRPADEEHPFGYGRVRYVYAFIVSIVLFSVGGLFAVYEGV
                     HKVSHPEKLTSPQWAIGVLLVSVLLESFSL"
     gene            complement(393..764)
                     /locus_tag="VEP73_02035"
     CDS             complement(393..764)
                     /locus_tag="VEP73_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25253.1"
                     /translation="MAMSVARARPGGAILAVLLMVTVGSVAVTLQPAPRRGLAAGVFL
                     VALLAGLGAASRLPRRRGERTVMERINDSAFGVVLLAGSLIAAAALRPWLVALPALLT
                     GLLAGRFLRPAAPPTDEGEGP"
     gene            887..1612
                     /locus_tag="VEP73_02040"
     CDS             887..1612
                     /locus_tag="VEP73_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25254.1"
                     /translation="MALVLQGTVTTFDPALPVAADAAVYVGDDGRIEAVQHRADPAPA
                     GYGPAPRVRTGGTIYPGLIDLHSHLAYNCRSLWAPPGRTEPFTSRDQWPRLPAYATDI
                     RQPTLALTQAASKAVLKYVEAKAVFGGVTAIQGSAVLTRPYEGWLARNVEFETFATGR
                     RSVFQSVRTLDGDAFATAREHMATGDAFIYHLAEGTAPRLVREYEDLRTHDCLFPQLV
                     AIHATALGGPEYDDWGPHGSSMV"
     gene            1658..>2232
                     /locus_tag="VEP73_02045"
     CDS             1658..>2232
                     /locus_tag="VEP73_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT25255.1"
                     /translation="MAAREAGIRVCLGADWAPSGSKQLLGELKVAHLWNRHPQGLGGA
                     FSDQELCEMVTAHPADALGWGDRIGRVRAGCFADLLALPDRVPDPYGNLIRATEREVR
                     LVLVGGRPTYGTVGMMGAAGAAGAEPLTVAGRRRAIAMLNPAVKDADMTWRQVLAALR
                     AARADPVRAQARQLALAAGEEPLRIIPDDPGG"
BASE COUNT          316 a          866 c          794 g          256 t
ORIGIN      
        1 caacgagaag gactcgagca ggaccgacac cagcagcacc ccgatcgccc actgcggcga
       61 ggtcagcttc tccgggtggg acaccttgtg cacgccctcg tagaccgcga acagcccgcc
      121 gaccgagaac agcacgatgg acacgatgaa cgcgtagacg tacctgaccc ggccgtaccc
      181 gaacggatgc tcttcgtcgg ccggccgctt ggagcggcgc ccacccacca gcagcagcac
      241 ctggttgccg gagtcggcca ccgagtggat cgcctcggcg agcatcgacg acgcgccggt
      301 gaacaggaac gcgacgaact tggcgacggc gatcccgagg ttggcgagga aggccgcgac
      361 gatcgcgcgg gtgccgccct ccccttcctc cttcacgggc cctcgccctc gtcggtgggt
      421 ggcgcggccg ggcgcaggaa cctgccggcg aggaggccgg tcaggagcgc gggcagcgcg
      481 accagccacg ggcgcagcgc ggcggcggcg atcagcgagc cggccagcag caccacaccg
      541 aacgccgagt cgttgatgcg ctccatcacc gtccgctcgc cgcggcgcct cggcagccgg
      601 ctggcggcgc cgagcccggc gagcagggcg accaggaaca cgcccgcggc cagtccgcgc
      661 cgcggggctg gctggagcgt gaccgcgacg ctgccgaccg tgaccatgag caggacggcg
      721 aggatcgccc cgcccggccg tgcccgcgca acgctcatcg ccatggattc cccagtccgg
      781 gtccgccacc ggtccgcggc ggacctgccg tagcctaccc gctccggggc cgggcgctca
      841 ccccggggcc ccggacgata agatcccggc cgaggggtgg ccgccgatgg cgctcgtgct
      901 gcaggggacc gtcacgacgt tcgacccggc gctgccggtg gcggccgacg ccgccgtcta
      961 cgtcggcgac gacggccgga tcgaggcggt ccagcaccgc gccgacccgg ctccggccgg
     1021 gtacgggccg gccccgcggg tgcggaccgg cgggacgatc tacccaggcc tgatcgacct
     1081 gcacagccac ctcgcgtaca actgccggtc gctgtgggcg ccgcccgggc ggacggagcc
     1141 gttcaccagc cgcgaccagt ggccccggct ccccgcgtac gccaccgaca tccgccagcc
     1201 caccctcgcc ctcacccagg cggcgagcaa ggccgtgctc aagtacgtcg aggccaaggc
     1261 cgtattcggc ggggtgacgg ccatccaggg ctcggccgtc ctcacccgcc cctacgaggg
     1321 ctggctagcc aggaacgttg agttcgagac cttcgcgact ggccggcgca gcgtcttcca
     1381 gtccgtgcgc acgctcgacg gggacgcctt cgccacggca cgggagcaca tggcgaccgg
     1441 ggacgcgttc atctaccacc tggccgaggg caccgcgccg cggctcgtcc gcgagtacga
     1501 ggacctgcgc acccacgact gcctgttccc gcagctggtc gccatccacg cgaccgcgct
     1561 cggcgggccc gagtacgacg actggggccc ccacggcagc tccatggtgt gatcgccgtt
     1621 ctccaacctc tggctgtacc gccacacgag cgacgtggtg gcggccaggg aggcggggat
     1681 ccgcgtctgc ctcggcgccg actgggcgcc gtccggctcc aagcagctcc tcggtgagct
     1741 caaggtcgcc cacctgtgga accgtcatcc ccagggcctc ggcggggcct tctccgacca
     1801 ggagctgtgc gagatggtca cggcccaccc ggccgacgcg ctcggctggg gcgaccgtat
     1861 cggccgggtc agggccggct gcttcgccga cctgctcgcc ctgcccgacc gcgtgcccga
     1921 cccgtacggg aacctgatcc gggccaccga gcgcgaggtg cgcctggtcc tcgtgggggg
     1981 ccgcccgacc tacgggacgg tcggcatgat gggcgccgcg ggcgcggccg gcgccgagcc
     2041 gctcacggtc gccggccggc ggcgcgcgat cgccatgctg aacccggccg tcaaggacgc
     2101 cgacatgacc tggcgccagg tcctcgccgc gctccgggcg gcgcgggccg acccggtcag
     2161 ggcccaggcc cggcagctcg cgctggccgc cggcgaggag ccgctccgca tcatccccga
     2221 cgacccgggc gg
//
LOCUS       DASPYY010000137         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2129830, whole genome shotgun sequence.
ACCESSION   DASPYY010000137 DASPYY010000000
VERSION     DASPYY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129830"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VEP73_02050"
     CDS             <1..398
                     /locus_tag="VEP73_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT25256.1"
                     /translation="AATARAGVRVVGVVTDAAPAMRAALDAGRVVAVRERPTLADGVA
                     GGLEAGSVTVDLARLAHDVVVVPEAEVAAAVRYLAREHGLVAEGAGAMAVAALRAGLV
                     PVKGRPVALVTGRNIALPLLARVLGEGPP"
     gene            395..1399
                     /locus_tag="VEP73_02055"
     CDS             395..1399
                     /locus_tag="VEP73_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT25257.1"
                     /translation="MRRARLGRSGVEVTELGFGGAPIGNLYAELDDAGAHEAVDAAWD
                     AGVRYFDTAPHYGLGLSERRLGEALRGRDRASYTLSTKVGRLLVPDPGPDGGRDLEGF
                     AVPATHRRVWDFSREGVRRSLEASLERLGLDRVDVLYLHDPDDHRRWVLEEGYPAIAG
                     LRDQGVVGAVGAGMNQWQMLADLVRETDLDVVMLAGRYTLLEQPALPVLLPLCRARGV
                     AVVAAGVFNSGLLARSRPAAGATYDYASAPAALVERAGRIADVCERHGATLPQAALQF
                     PLGHPAVAGIVSGVRSAAELRRNAELLAAPVPAALWSELRAEGLLAEDVPVPGPAPGR
                     "
     gene            1504..>1822
                     /locus_tag="VEP73_02060"
     CDS             1504..>1822
                     /locus_tag="VEP73_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25258.1"
                     /translation="MDRSQTIDRRGGAGARLELDGSLSPRRVARRLALVVAVLTLLSL
                     MGQLAVLYLPDFPLRDFTARLFNVDQEVNLPSIYSALALLACSVLLALIARSERLARG
                     PRQG"
BASE COUNT          184 a          709 c          693 g          236 t
ORIGIN      
        1 tcgccgcgac ggccagggcg ggcgtgcgcg tcgtcggggt cgtcaccgat gccgcgccgg
       61 cgatgcgggc agcgctcgat gccgggcgcg tcgtcgcggt ccgggagcga cccacgctcg
      121 ccgacggcgt cgccgggggg ctcgaggcgg ggagcgtcac cgtcgacctc gcccggctcg
      181 cccacgacgt cgtcgtcgtg ccggaggcgg aggtcgccgc cgcggtccgc tacctggccc
      241 gcgagcacgg cctggtggcc gagggcgccg gcgccatggc cgtcgccgcg ctccgcgccg
      301 gcctcgtgcc ggtaaagggc cggccggtcg cgctcgtcac cgggcgcaac atcgctctgc
      361 cgctgctcgc ccgggtgctc ggggagggcc ccccgtgaga cgggcgcggc tcgggcggag
      421 cggggtcgag gtcaccgagc tcggcttcgg cggcgcgccg atcggcaacc tctacgccga
      481 gctggacgac gccggcgcgc acgaggccgt cgacgccgcg tgggacgccg gcgtccgcta
      541 cttcgacacc gcgccgcact acggcctcgg cctgtccgag cggcggcttg gagaggcgct
      601 gcgcggccgc gaccgcgcgt cgtacacgct gtccaccaag gtcggccggc tgctggtgcc
      661 ggacccgggc ccggacggcg gccgggacct cgaggggttc gccgtgccgg ccacccaccg
      721 gcgggtgtgg gacttcagcc gcgagggcgt gcgccgctcg ctcgaggcga gcctcgagcg
      781 gctcggcctc gaccgggtgg acgtcctgta cctccacgat cccgacgacc atcgccgctg
      841 ggtgctcgag gagggctacc ccgcgatcgc cggcctgcgg gaccagggcg tcgtcggcgc
      901 ggtcggggcc ggcatgaacc agtggcagat gctcgccgac ctcgtccgcg agaccgacct
      961 ggacgtcgtc atgctcgccg gccgctacac gctcctggag cagccggccc tgccggtgct
     1021 cctgccgctg tgccgggcgc gcggcgtcgc cgtcgtcgcc gccggcgtgt tcaactcggg
     1081 tctgctggca cggtcccggc ccgcagcggg cgcgacctac gactacgcca gcgcccccgc
     1141 cgcgctcgtc gagcgggcgg gccgcatcgc ggacgtgtgc gagcgccatg gcgccacgct
     1201 gccgcaggcc gccctgcagt tcccgctcgg gcacccggcc gtcgccggca tcgtctccgg
     1261 cgtccgctcc gccgcggagc tgcggcgcaa cgccgagctg ctcgccgcgc cggtcccggc
     1321 cgcgctatgg tcggagctgc gcgccgaggg cctgctggcc gaggacgtgc ccgttcccgg
     1381 gccggccccc ggacgctgac cggcggcatc cccggcccgc cccggatgcg gcagtgccgg
     1441 gttggcgccg ctctgcaacg ggcatccagg aatcgggcgc aggcgcacac aaggagaccg
     1501 ggcatggatc gctcccagac gatcgatcgg cgcggcggag cgggcgcgcg cctggagctc
     1561 gacggcagcc tctcgcctcg gagggtcgcg cgccgcctcg cgctcgtcgt ggcggtcctg
     1621 acgctgctct cgctcatggg gcagctcgcg gtgctctacc tgccggactt ccccctgcgg
     1681 gacttcaccg cgcgcctgtt caacgtcgac caggaggtga acctcccctc catctactcg
     1741 gcgctcgcgc tgctggcctg ctcggtcctg ctggcgctga tcgcacgctc ggagcggctg
     1801 gccaggggtc cgcgccaagg cc
//
LOCUS       DASPYY010000138         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2411550, whole genome shotgun sequence.
ACCESSION   DASPYY010000138 DASPYY010000000
VERSION     DASPYY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411550"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEP73_02065"
     CDS             <1..180
                     /locus_tag="VEP73_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25259.1"
                     /translation="LAAGATRAVVVRAVTGSGDPAAAVAALRRRLAAAAAAPARRPAG
                     PPPASRAPGSTRPRR"
     gene            248..1273
                     /locus_tag="VEP73_02070"
     CDS             248..1273
                     /locus_tag="VEP73_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT25260.1"
                     /translation="MPQPRLKDVAAAAGVHAATASRALNERTAGMISPETVERVRRAA
                     EELGYQVNGMARALKTRRSLAVGMLVPDITNPFFPPIVRGAEDALAAAGYTLVLGNTD
                     NDEAKERRHLSQMAQSQVDGFLLATARLRDPAIDRLRRGEVPFVLVNRTVERGGLSAV
                     IPDDRAAMLLAVEHLHGLGHRRIGYVGGPLDTSTGARRAEGFRAAMRLHGLRDDAATL
                     AAVFNATAGRRAAAELLAVRPRPTAIVAANDLLALGTLDAAAEAGLECPLGVSVVGVN
                     DIPMADRLSPSLTTARVPEYELGRRSARLLLAHLERPDRDPETVMLAPELIVRGSTAP
                     PRAPIGS"
     gene            1372..2613
                     /locus_tag="VEP73_02075"
     CDS             1372..2613
                     /locus_tag="VEP73_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYT25261.1"
                     /translation="MSRRRWTATLALALAAALAAGACSSGGSKGSGSGAAQSSKPLRI
                     GISLSFSGDFSDPAKLVRRGYELWADGVNARGGLLGRKVELKVVDDASSPTQVVSNYQ
                     NLITRDKVDLVFGPFSSLLTIPSARIANRYGYAFPEPEGGGPKVFAAKLPNLFFVQPA
                     PALSSGDVFARYVLSLPAAERPKTAAYPSLDDPFASPIADRVRERFEAAGIRTVYKTI
                     YPAETVDLTPIIQRVAAAKPDVVVSGTQSEDAYAQVKAMIQLGFSPKMLFMSNGANSP
                     VEFPDKVGKGNTEGIFSSGTWSPDAKTNGNAEFVAAYAKKYGGGPRDIDGNTADAYAT
                     GQLLEEVAAKTRSLDNTTIIKTLHTGSWPTIQGELSWDADGATRGSDLLLQWIGGSLY
                     PVYPQSVATHAPVSHKGAWRH"
     gene            2639..>3198
                     /locus_tag="VEP73_02080"
     CDS             2639..>3198
                     /locus_tag="VEP73_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT25262.1"
                     /translation="MHTIVQAAILGLSAGAVYALMASGLTLVFGVMRVVNVAQGALVI
                     LAAYLSYALFSRLHVDPFLSILFVTPVLFALGAGLQVVFLRRLRQDEQELSLLVMFAV
                     ALGIEGVLSVIWKTTYRSTTTSYANRSLRLFGYQVSVVRVLAFVLSVGVLAALWVLLE
                     RTRFGRAVRATVQNPTSARLLGVDAQR"
BASE COUNT          434 a         1259 c         1070 g          435 t
ORIGIN      
        1 ctcgccgcgg gcgcgacccg ggccgtggtg gtccgcgcgg tcaccggctc gggcgacccg
       61 gccgcggccg tggccgcgct ccggcgccgg ctcgccgccg cggcggccgc tccagcccgg
      121 aggccggccg ggccgccgcc cgcgtccagg gcgccgggct cgacccggcc gcgtcgttga
      181 ccggcgctcc cacctcgggt accgtctgct tgcaatcgat tgccatccgg ggggaggtgg
      241 gcggtccttg ccgcagccgc gcctgaagga cgtcgccgcg gcggccgggg tccacgccgc
      301 caccgcgtcg cgcgccctca acgagcgtac cgccggcatg atcagcccgg agacggtcga
      361 gcgggtccgc cgggccgccg aggagctcgg ctaccaggtc aacggcatgg ccagggcgct
      421 caagacgcgc cggtcgctgg ctgtcggcat gctcgtcccc gacatcacca acccgttctt
      481 cccgccgatc gtgcgcggcg ccgaggacgc cctggcggcg gccgggtaca ccctggtgct
      541 cggcaacacc gacaacgacg aggccaagga gcgccgccac ctgagccaga tggcgcagag
      601 ccaggtcgac gggttcctcc tggcgaccgc gcgcctgcgc gacccggcca tcgaccgcct
      661 ccggcgcggc gaggtcccgt tcgtgctcgt caaccggacc gtggaacggg gcggcctctc
      721 cgccgtgatc cccgacgacc gtgccgccat gctgctcgcg gtcgagcacc tccacggcct
      781 ggggcaccgg cgcatcggct acgtgggcgg cccgctcgac acctcgaccg gggcgcgccg
      841 cgccgagggg ttccgggccg ccatgcggct gcacgggctg cgcgacgacg ccgccacgct
      901 cgcggccgtg ttcaacgcga ccgccgggag gcgcgccgcc gcggagctcc tcgccgtgcg
      961 gccccgcccg acggcgatcg tggcagccaa cgacctgctc gccctcggca cgctcgacgc
     1021 cgcggccgag gcgggcctcg agtgcccgct cggcgtctcg gtcgtcgggg tcaacgacat
     1081 ccccatggcc gaccgcctct cgccgtcgct cacgaccgcc cgcgtgcccg agtacgagct
     1141 cggccgcagg tcggcgcgcc tgctgctcgc ccacctcgag cggccggacc gcgaccccga
     1201 gaccgtcatg ctcgcgcccg agctgatcgt gcgcgggtcg acggcgccgc cgcgggcgcc
     1261 gatcgggtct tgacaagact tccgttcccg cgtagcgtct gcaaacgatt gcacctcagg
     1321 ggaggaacgc acctcaggac aggaacgcac ctccagggga ggagaccagc catgtcgcga
     1381 cgccgctgga cggcgaccct ggccctcgcg ctcgccgccg cgctcgccgc cggcgcctgc
     1441 tcgtccggag ggtccaaggg ctcgggctcc ggcgcggcac agagctccaa gccgctccgc
     1501 atcgggatct cgctgtcgtt ctcgggtgac ttctccgacc cggccaagct ggtgcggcgc
     1561 ggctacgagc tctgggccga cggcgtgaac gcaaggggcg gcctgctcgg ccgcaaggtc
     1621 gagctcaagg tcgtcgacga cgcgtccagc cccacccagg tggtcagcaa ctaccagaac
     1681 ctgatcaccc gcgacaaggt cgacctggtc ttcgggccgt tctcgagcct gctcacgatc
     1741 ccgtccgccc ggatcgccaa ccgctacggc tacgccttcc ccgagccgga gggcggcggc
     1801 cccaaggtgt tcgcggccaa gctccccaat ctcttcttcg tgcagccggc cccggccctg
     1861 tccagcggtg acgtgttcgc caggtacgtc ctctcgctgc ccgcggcgga acggccgaag
     1921 accgcggcct acccgtcgct cgacgacccg ttcgcctccc cgatcgccga cagggtccgc
     1981 gagcggttcg aggcggccgg catcaggacc gtgtacaaga ccatctaccc ggccgagacg
     2041 gtcgacctca ccccgatcat ccagagggtc gccgccgcaa agcccgacgt ggtcgtctcc
     2101 ggcacccagt ccgaggacgc ctacgcgcag gtcaaggcca tgatccagct cggcttcagc
     2161 ccgaagatgc tgttcatgtc caacggcgcg aactcaccgg tcgagttccc cgacaaggtc
     2221 ggcaagggca acaccgaggg catcttctcg agcggcacgt ggtcgcccga cgccaagacg
     2281 aacggcaacg ccgagttcgt ggccgcctac gccaagaagt acggcggcgg ccctcgcgac
     2341 atcgacggca acaccgccga cgcctacgcc accgggcagc tcctcgagga ggtcgccgcg
     2401 aagaccaggt cgctcgacaa caccacgatc atcaagaccc tccacaccgg gagctggccc
     2461 accatccagg gcgagctgtc gtgggacgcc gacggggcga cgagaggcag cgacctgctc
     2521 ctgcagtgga tcggcggctc gctctacccg gtctacccgc agagcgtggc gacccacgcc
     2581 ccggtcagcc acaagggggc ctggcggcac tagcccgcgc cgcggcaacg gaggcgacat
     2641 gcacaccatc gtgcaggccg cgatcctcgg cctctcggcg ggcgccgtct acgcgctcat
     2701 ggccagcggc ctgaccctcg tcttcggcgt catgcgggtc gtcaacgtgg cccagggcgc
     2761 cctggtgatc ctggccgcct acctgagcta cgccctgttc agccgcctgc acgtcgaccc
     2821 gttcctgtcc atcctcttcg tcaccccggt cctgttcgcg ctcggggccg ggctgcaggt
     2881 cgtcttcctc cgccggctcc gccaggacga gcaggaactc tcgctgctcg tcatgttcgc
     2941 cgtcgcgctc ggcatcgaag gggtgctgag cgtgatctgg aagaccacct accgctcgac
     3001 gacgaccagc tacgccaacc gctccctccg gctcttcggc tatcaggtct cggtcgtccg
     3061 ggtcctcgcc ttcgtgctct cggtcggggt cctcgccgcc ctgtgggtgc tgctcgagcg
     3121 cacccggttc ggccgggccg tccgggccac cgtgcagaac ccgacctcgg cccgcctcct
     3181 cggggtcgac gcccagcg
//
LOCUS       DASPYY010000139         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1850423, whole genome shotgun sequence.
ACCESSION   DASPYY010000139 DASPYY010000000
VERSION     DASPYY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850423"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VEP73_02085"
     CDS             complement(<1..562)
                     /locus_tag="VEP73_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25263.1"
                     /translation="MDVRLTNAYVADAKLVADGLVQPEDAMAVFVGRAGRGRVAPFVV
                     ERSYTGAGGWYTESMALLAANGDVAWRGPSKAITLRGSNKIDTFTEEVHGVTVAGDDD
                     HELVFLLSDGRAGADEVGRVPLAVLDEDPPGSAAPETAVLDEALKKSTVVWIEVPGPD
                     GTGGRTVPAWYGTLDGRVYVLTGGSEQ"
     gene            639..1175
                     /locus_tag="VEP73_02090"
     CDS             639..1175
                     /locus_tag="VEP73_02090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25264.1"
                     /translation="MGVTGTPPDVVCRAVVRETDEVRGFLAREWQVADRGIFGADLDW
                     TSRPIVVEARSGRELVGVAVGEAVAGMARLSDVLVAGPRQGAGVGGRLVELFCERAAA
                     AGAGRCFLRCPDTESHRRFYERHGFVQIARIPRYYHGRDFLEYMREPLARPPAAGEAP
                     TAGERAARPRGRRRPRGR"
     gene            complement(1197..1385)
                     /locus_tag="VEP73_02095"
     CDS             complement(1197..1385)
                     /locus_tag="VEP73_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25265.1"
                     /translation="MKLLLEILIAIFLHPIAFILAVVNIAGRSDLGTFKKVVWAVVSL
                     VWGVGPILYIFLADGDLW"
     gene            1504..>1957
                     /locus_tag="VEP73_02100"
     CDS             1504..>1957
                     /locus_tag="VEP73_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005307906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYT25266.1"
                     /translation="MLVEHRGARPSVHPTAYVAPNAVLCGDVRVGEDARVLFGAVLTA
                     EDGTVEVGARCVVMEHALLRGRADHPVRIGDDVLVGPHAHVNGAEIGDGAFLATGVSV
                     FPGARVGARAEVRVGAVVHVNSSLPPEATVPIGWVAVGVPAQVLPPERD"
BASE COUNT          232 a          696 c          755 g          274 t
ORIGIN      
        1 gctgctccga cccgccggtg agcacgtaga cgcggccgtc gagggtcccg taccaggcgg
       61 ggaccgtccg tccgccggtg ccgtccggac cggggacctc gatccagacg accgtgctct
      121 tcttcagggc ctcgtcgagc acggcggtct cgggcgcggc gctgcccggc gggtcctcgt
      181 cgaggacggc gagcgggacg cggccgacct cgtcggcgcc cgcgcggccg tcgctcagca
      241 ggaagaccag ctcgtggtcg tcgtcgccgg ccacggtgac cccgtgcacc tcctcggtga
      301 aggtgtcgat cttgttcgag ccgcgcagcg tgatcgcctt cgacgggccg cgccacgcca
      361 cgtccccgtt cgccgcgagc agggccatgg actccgtgta ccagccgccc gcgccggtgt
      421 aggagcgctc cacgacgaac ggcgccaccc ggccccggcc ggcccggccg acgaacacgg
      481 ccatcgcgtc ctcgggctgg accagcccgt cggcgaccag cttggcgtcg gcgacgtagg
      541 cgttggtgag acggacgtcc attgagctcc cttcggatgc cggcggtcgg cggtcatgat
      601 gatatgggaa tgtcgacgtc gtcagggagg ttggcgcggt gggcgtgacg ggcacgccgc
      661 cagatgtcgt gtgccgggcc gtcgtgcggg agaccgacga ggtccgcggc ttcctcgccc
      721 gcgagtggca ggtggccgac cggggcatct tcggcgccga cctggactgg acgagccggc
      781 cgatcgtcgt cgaggcccgg tcgggccggg agctcgtcgg cgtcgccgtc ggcgaggccg
      841 ttgccggcat ggccaggctg agcgacgtgc tggtggccgg gccgcgccag ggcgccgggg
      901 tgggcgggcg cctggtcgag ctgttctgcg agcgggccgc ggcggccggc gccggccgtt
      961 gcttcctgcg ctgccccgac accgagtcgc accggcgctt ctacgagcgg cacgggttcg
     1021 tccagatcgc ccgcatcccg cgctactacc acggcaggga cttcctcgag tacatgcgcg
     1081 agccgctcgc cagaccgcct gcagcgggcg aggctcccac ggccggggag cgggcggcgc
     1141 ggccgcgcgg ccggcgccgg ccgcgcggca ggtgaccggc ttggcctgga cccgtcctac
     1201 cagaggtcgc cgtcggccag gaagatgtag aggatcggcc cgacgcccca gacgaggctg
     1261 acgacggccc agacgacctt cttgaaggtt ccgaggtcac tgcgtcccgc gatgttgacg
     1321 accgccagga tgaaggcgat cgggtggagg aagatcgcga ttaggatctc gagtagcagt
     1381 ttcatcgctg gccgctcccg tggtcgtcgt cccggcaggg tcaccgtacc cggtcgccac
     1441 catggcgtgc gagcgggccg gccggcgctg gcgccgggcc ggctcatgcg ccacgatggc
     1501 gccatgctcg tcgagcaccg gggtgcgcgg ccgtccgtgc accctaccgc ctacgtggcc
     1561 ccaaacgccg tgctctgcgg tgacgtgcgg gtcggggagg acgcgcgcgt gctgttcggc
     1621 gccgtgctga ccgccgagga cggcacggtc gaggtcggcg cacgctgcgt ggtcatggag
     1681 cacgcgctgc tgcgcgggcg tgcggaccac ccggtgcgca tcggggacga cgtgctcgtc
     1741 ggcccgcacg cgcacgtcaa cggcgcggag atcggcgacg gggcgttcct ggccaccggc
     1801 gtgtcggtct tccctggcgc ccgcgtcggc gcgcgcgccg aggtgcgggt gggcgcggtc
     1861 gtccacgtga actcgagcct cccgcccgag gcgacggtcc ccatcggctg ggtggcggtc
     1921 ggcgttcccg cccaggtcct gccgccagag cgcgacg
//
LOCUS       DASPYY010000140         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1007952, whole genome shotgun sequence.
ACCESSION   DASPYY010000140 DASPYY010000000
VERSION     DASPYY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007952"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VEP73_02105"
     CDS             <1..342
                     /locus_tag="VEP73_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HYT25267.1"
                     /translation="NGDERSGAEIVRRALELPAQWIARNAGYEGAVVVARIRELSDNQ
                     GFDATTGEYVDLVKAGVIDPAKVTRSAVQNAASIAALLLTTETLVVDKPEEKDEAVGA
                     GHGHGGGYPGM"
     gene            595..1326
                     /locus_tag="VEP73_02110"
     CDS             595..1326
                     /locus_tag="VEP73_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT25268.1"
                     /translation="MFPHQRCLRPDERSAEIWFDAPDGTRLSGVTLGSGTTGVLLAHQ
                     HWFNLCSWMPFARDLSRTGYRVLAFDFRGSGASPAPDQRVARNLDQDVAGGVRELRRL
                     GAQRVVLVGASMGATASLVEAAHPIHPPELPVAGVVSVSGPTHFYEMNALAAVERLHA
                     PLLLIATAQDGRFTAAARSFYRAAPSVDKRLVILSGKGHGTGLLTSSTHSARLRSLVL
                     GFIREHAGPMPQTAAASQRTIRPSR"
     gene            complement(1299..1565)
                     /locus_tag="VEP73_02115"
     CDS             complement(1299..1565)
                     /locus_tag="VEP73_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25269.1"
                     /translation="MIDMVIGRDDAATVCGLLHDIGRGSGAPPDLRDAAIYWAAEVGP
                     DMERGDLQTVAWLLRDASASGRVSQVRRDQARYWAAYLEGRMVR"
     gene            1767..>2938
                     /locus_tag="VEP73_02120"
     CDS             1767..>2938
                     /locus_tag="VEP73_02120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYT25270.1"
                     /translation="MRVTVIGAGHVGLVTAVCLARTGHDVVADDDDRAKLELLRQGRA
                     WFYEPGLQDLMTEALAAGRLRFTSNKAEAVRHGEVIFVSVGTPSREDGSPNLRFVEAV
                     ARDVARHLPVGEFRLVCEKSTVPVQTGLRVAQVMRREAPAGADYEVASNPEFLREGSA
                     VQDTLHPDRIVVGTESERGTGVLHELYRPIIESTGCRFLATDRATAELIKHASNAFLA
                     TKISFINSVAAVCERSGADVELVAQGMGFDPRIGPQFLKAGAGYGGSCFPKDVAAFAH
                     RSRELGVDFGILREVAKVNLEARRNIVEKVRDALWHLEGKRVGVLGLAFKPDTDDLRE
                     APAMDVVRGLLDGGAEVVAYDPVAREQAAAHMPGLQVVAKAHEVADGAHALVLLTE"
BASE COUNT          396 a         1106 c         1020 g          416 t
ORIGIN      
        1 aacggcgacg agcggagcgg ggccgagatc gtgcgccgcg cgctcgagct gccggcgcag
       61 tggatcgcca ggaacgccgg gtacgagggc gccgtggtcg tcgcccggat ccgcgagctg
      121 tccgacaacc agggcttcga cgcgaccacc ggcgagtacg tcgacctggt caaggcgggc
      181 gtcatcgacc cggccaaggt gacccggtcc gcggtgcaga acgccgcgtc gatcgccgcc
      241 ctcctgctga ccaccgagac cctggtcgtc gacaagccgg aggagaagga cgaggccgtc
      301 ggcgccggcc acggtcacgg tggtggctac ccgggcatgt gaccccgtca cgccacgcga
      361 ggggccccgg ccgacggccg gggcccctcg acttgcagcc acgtcgtaag atacgaatca
      421 gcccggttcg tccagcgccg cggttctttg cgcccttctt cggaggtcaa cctgtcagca
      481 ggccgtcggg gcgtcctcgc cgcccttctc gttctcctcg ccgcctgctc gaccggtccc
      541 gacaggacga cggccgcatc cgaccggatc ggggcctcca tccctgcgtc cgaggtgttc
      601 ccgcaccaac gctgcctgcg gccggacgag cgctcggcgg agatctggtt cgacgcccct
      661 gacgggaccc ggctgtccgg ggtgacgctc gggtcgggca ccaccggcgt cctgctcgcc
      721 caccagcact ggttcaacct ctgcagctgg atgccgttcg ccagggacct gtcgcgcacc
      781 gggtaccggg tgctggcctt cgacttccgc gggtccggcg cctctcccgc tcccgaccag
      841 cgggtcgcca ggaacctgga ccaggacgtc gcaggcggcg tcagggagct gcgtcggctc
      901 ggggctcagc gcgtcgtgct ggtcggcgcc tccatgggcg ccaccgcgtc cctcgtcgag
      961 gccgcccatc ccatccaccc gccggagctc ccggtggccg gggtcgtcag cgtgtcgggc
     1021 ccgacccact tctacgagat gaacgcgctg gcggccgtcg agcggctgca tgcgccgctg
     1081 ctgctgatcg ccaccgcgca ggacggcagg ttcaccgccg ccgcccggtc cttctaccgg
     1141 gccgccccgt ccgtcgacaa gcgcctcgtg atcctgtccg ggaaggggca tggcaccggc
     1201 ctgctgacct ccagcacgca ctcggcccgg ctgcgctccc tggtcctggg cttcatcagg
     1261 gagcacgccg ggccgatgcc gcagaccgcc gccgccagtc agcggaccat acggccctcc
     1321 aggtaggcgg cccagtagcg ggcctggtcg cgccggacct gagagacccg gccgctggcg
     1381 ctggcgtcgc gcagcagcca ggcgaccgtc tgcaggtcgc cccgctccat gtccgggccg
     1441 acctcggccg cccagtagat ggcggcgtct ctcaggtccg gcggggcccc gctgccgcgg
     1501 ccgatgtcgt gcagcagccc gcacacggtc gccgcgtcgt cccgcccgat caccatgtcg
     1561 atcatcagcc ggctccttcc cgggtcgggc ggcggcgggt cgcgcggcca cctcgagatt
     1621 tgtcgaacgt atgttcgaac agttgccggt caccgtcaag ctcggcccgg taggggcacc
     1681 ccacggattg gccgaggctg gcaccaccag ggcaaggcgc cggtagaatc tcgtcttccg
     1741 gtcagccact cgaggagatc caccacatgc gcgttacggt catcggggcc ggccacgtcg
     1801 gactcgtcac tgccgtctgc ctggccagga ccggccacga cgtcgtggcc gacgacgacg
     1861 accgggccaa gctcgagctg ctgcggcagg gcagggcctg gttctacgag ccggggttgc
     1921 aggacctgat gaccgaggcg ctcgccgccg ggcggctgcg gttcacctcc aacaaggccg
     1981 aggcggtccg ccacggcgag gtgatcttcg tctcggtggg cacgcccagc cgcgaggacg
     2041 gcagccccaa cctcaggttc gtcgaggcgg tcgcccgcga cgtcgcccgc cacctgcccg
     2101 tgggcgagtt ccgcctggtc tgcgagaagt ccacggtgcc ggtgcagacc ggcctgcggg
     2161 tcgcccaggt gatgcggcgc gaggcgcccg ccggcgccga ctacgaggtc gcctcgaacc
     2221 ccgagttcct ccgcgagggc tcggcggtcc aggacacgct ccaccccgac cggatcgtcg
     2281 tcggcaccga gtccgagcgg ggcaccggcg tcctccatga gctgtacagg ccgatcatcg
     2341 agtcgaccgg gtgccggttc ctggccaccg accgggccac cgccgagctc atcaagcacg
     2401 cctcgaatgc gttcctcgcc accaagatca gcttcatcaa ctcggtcgcc gcggtctgcg
     2461 agcggtcagg ggccgacgtc gagctcgtcg cccagggcat ggggttcgac ccgcgcatcg
     2521 gcccccagtt cctcaaggcc ggcgccggct acggcgggag ctgcttcccc aaggacgtcg
     2581 ccgccttcgc ccaccgctcg cgcgagctcg gcgtcgactt cggcatcctc cgcgaggtcg
     2641 ccaaggtcaa cctggaggcc cgtcgcaaca tcgtcgagaa ggtgcgcgac gcgctgtggc
     2701 acctcgaggg caagcgggtc ggcgtgctcg ggctcgcctt caagcccgac accgacgacc
     2761 tgcgcgaggc gccggccatg gacgtggtcc gcggcctgct cgacggcggc gccgaggtgg
     2821 tcgcctacga cccggtcgcc cgtgagcagg ccgcggccca catgccaggg ctgcaggtgg
     2881 tcgccaaggc ccacgaggtg gccgacggtg cccatgcgct cgtgctgctg accgagtg
//
LOCUS       DASPYY010000141         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2552904, whole genome shotgun sequence.
ACCESSION   DASPYY010000141 DASPYY010000000
VERSION     DASPYY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552904"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2262
                     /locus_tag="VEP73_02125"
     CDS             <1..2262
                     /locus_tag="VEP73_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HYT25271.1"
                     /translation="TGKLPAPGQIGQTGAGDLTPDSDQTLQHGRLETVDDALDQYTDS
                     IGDLLNVNSQIAFDNAGDDRELLRSLTTLVSLSRLKEFNDQERGFISRVLYNGRFQSG
                     DFARYAAIVAGENLWLAQFQASATSDQRRVFGGTVTGPDVVQAQKFQQSILRGENAQR
                     INIDPTRWYAAMSSKVELLRKVELGVNGEVTDASSRLKASADRRALLYSLALTVVLWL
                     AVGLSLFTARSMVGPLRMLKDAADDVARRKLPSVVERLQRSERVDLDAETTPIGIISR
                     DEIGQVAEAFNEVHRTAVHVAGEQAALRTSVGDMFLNLARRSQSLVDRQLELIDDLER
                     GETDPDALENLFRLDHLATRMRRNAENLIVLSGSDPARRWSEAVALVDIVRAAIAEVE
                     DYTRVELLPIEPLGVAGQAVGDVVHLLAELIENATAFSPPGTKVQIAGQNVSSGYVIE
                     VEDRGLGMTDAELVEANERLANPPMIDFALSRMLGIFVVSRLASRYGIKVQLRHSWYG
                     GVTALVLFPEQIIVRTVGDGGPLVPAPAQVTHGQSRTELPSPAAPSLAAPSLAPPPPN
                     GTLPSNGVSTSAGASRGDSLPIFEAARSDWFETGSAAAHLPLRRHAAQLNGEPEGAPV
                     GPTRNRNGDGAAPEPPPPPPPPPDLVVPPAPAPEPVREAPSPAPVTKAGLPRRIPKAN
                     LNPDIVDPSPSWERPQPAASSRSPGEVRSMLSSYRTGLERGRSAASSVVSSRERDAEE
                     PIGGDAASHAVEE"
     gene            2267..2689
                     /locus_tag="VEP73_02130"
     CDS             2267..2689
                     /locus_tag="VEP73_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HYT25272.1"
                     /translation="MTQLSKEAQNLNWLVTNFVRKVPGVAHTIVVSADGLLMAVSEKL
                     DRARADQLAAVASGLSSLTQGAARCFGGGAVNQTVVEMEKGFLFVMSVSDGSCLAVLA
                     SLNCDIGLIGYEMALLVARTGDVLTPALRAELQASLPR"
     gene            2705..3073
                     /locus_tag="VEP73_02135"
     CDS             2705..3073
                     /locus_tag="VEP73_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HYT25273.1"
                     /translation="MSTENGHGPGRFVRPYAMTGGRTRPSHMDLELELETLVSTTSLG
                     EQSVASLSLERRSIALLCRDVISVAEVSARLDIPLGVARVLVGDMADEGLVIVHRPAS
                     PSDRPDLALLERVLYGLRTI"
     gene            3078..3668
                     /locus_tag="VEP73_02140"
     CDS             3078..3668
                     /locus_tag="VEP73_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HYT25274.1"
                     /translation="MPGGSSTPAPTPVKIVIAGGFGVGKTTFVGAVSEIIPLTTEAVM
                     TSASVGVDDTNQVPGKTTTTVAMDFGRITLDTDLILYLFGTPGQDRFWFMWDDLVRGA
                     IGAVVLVDTRRLDACFAAVDFFEQRALPFIAAVNCFHGEALHAVDDVREALAIGRDVP
                     IVLCDARARDSAKATLIELVEHALARATAALDSSRA"
BASE COUNT          572 a         1433 c         1336 g          516 t
ORIGIN      
        1 accggcaagc ttcccgcgcc cggccagatc gggcagacgg gggccggcga cctcacaccc
       61 gactcggacc agacgctgca gcacggccgc ctcgagacgg tcgacgacgc gctcgaccag
      121 tacaccgaca gcatcgggga cctgctgaac gtcaacagcc agatcgcctt cgacaacgcg
      181 ggggacgacc gggagctgct gcggtccctc accaccctgg tgtcgctctc ccggctcaag
      241 gagttcaacg accaggagcg cggcttcatc agcagagtcc tctacaacgg ccgcttccag
      301 agcggcgact tcgccaggta cgccgccatc gtcgccggcg agaacctctg gctcgcgcag
      361 ttccaggcct cggcgacctc ggaccagcgg cgcgtcttcg gcggcaccgt gaccggtccc
      421 gacgtggtcc aggcgcagaa gttccagcag agcatcctgc gcggcgagaa cgcccagagg
      481 atcaacatcg accccacgcg ctggtacgcg gccatgagca gcaaggtgga gctgctcagg
      541 aaggtcgagc tcggcgtcaa cggcgaggtg accgacgcct cctcccggct caaggcgtca
      601 gccgaccggc gcgcgctgct gtacagcctc gccctcacgg tcgtcctgtg gctggcggtc
      661 ggcctgtccc tgttcaccgc ccgctcgatg gtcgggccgc tgcggatgct gaaggacgcg
      721 gccgacgacg tcgcccggcg caagctgccc agcgtggtgg agcggctgca gcggtccgag
      781 cgcgtcgacc tggacgccga gacgacaccg atcgggatca tctcgaggga cgagatcggc
      841 caggtggccg aggcgttcaa cgaggtccac cggacggccg tccacgtcgc cggcgagcag
      901 gcggccctgc ggacgagcgt cggcgacatg ttcctgaacc tcgcccggcg cagccagagc
      961 ctggtcgacc gccagctgga gctgatcgac gacctcgagc ggggcgagac cgaccccgac
     1021 gcgctcgaga acctcttccg gctcgaccac ctggccaccc gcatgcggcg caacgccgag
     1081 aacctgatcg tgctctccgg ctcggacccg gccaggcgct ggagcgaggc ggtcgccctg
     1141 gtcgacatcg tcagggccgc gatcgccgag gtcgaggact acacccgcgt ggagctgctc
     1201 ccgatcgagc cgctcggcgt ggccggccag gccgtcggcg acgtggtgca cctgctcgcc
     1261 gagctgatcg agaacgccac cgccttctcg ccgccgggca cgaaggtcca gatcgccggt
     1321 cagaacgtca gcagcgggta cgtgatcgag gtcgaggacc ggggcctcgg catgaccgac
     1381 gccgagctgg tcgaggccaa cgagcgcctc gccaaccccc cgatgatcga cttcgcgctc
     1441 tcccggatgc tcggcatctt cgtggtcagc cgcctcgcct cccggtacgg catcaaggtc
     1501 cagctgcgcc actcctggta cgggggcgtg accgcgctgg tcctcttccc cgagcagatc
     1561 atcgtcagga ccgtcggcga cggcggcccg ctggtgccgg cgcccgcgca ggtcacgcac
     1621 ggccagagcc gcaccgagct gccgtcgccg gccgcgccgt cgctggcggc gccatccctg
     1681 gcgcccccgc cgccgaacgg cacgctaccg tcgaacgggg tgtcgacgtc cgccggggcg
     1741 agccggggcg actccctgcc gatcttcgag gcggcgaggt cggactggtt cgagaccggc
     1801 tcggcggccg cgcacctgcc cctgcggcgc catgccgccc agttgaacgg cgagccggag
     1861 ggggcgccgg tgggccccac gcgcaaccgg aacggcgacg gggccgcccc cgagccgcca
     1921 ccgccaccac caccaccacc cgacctggtc gttccgcctg cccccgcccc agagccggtg
     1981 cgggaagcgc cctcgccggc gccggtgaca aaggccggcc tgccacgccg catccccaag
     2041 gcgaacctca accccgacat cgtcgacccg tcgccgtcct gggagcggcc gcagcccgcc
     2101 gcgagctccc gcagcccggg cgaggtccgc agcatgctgt cgagctaccg gaccggcctg
     2161 gagcgcgggc gcagtgcggc gtccagcgtc gtgtcgagca gggaaaggga cgcagaagag
     2221 ccgattggcg gcgatgccgc ttcgcacgcc gtcgaggagt gaccagatga cgcagctcag
     2281 caaggaagcc cagaacctca actggctcgt gaccaacttc gtcaggaagg tgcccggcgt
     2341 cgcccatacg atcgtcgtct ccgccgacgg gctgctcatg gcggtgtccg agaagctgga
     2401 ccgtgcccgg gcggaccagc tcgccgcggt cgcctcgggc ctgtccagcc tgacgcaggg
     2461 cgccgcccgc tgtttcgggg gcggtgccgt gaaccagacc gtcgtcgaga tggagaaggg
     2521 cttcctgttc gtgatgtcgg tcagcgacgg gtcgtgcctt gcggtgctgg catcgctcaa
     2581 ctgcgacatc ggtctgatcg gctatgagat ggccctgctc gtggcacgca cgggggacgt
     2641 gctcaccccg gccctgcgcg ccgagctcca ggcctcgctc ccgcgatgag cagaaggaac
     2701 ggccatgagt accgagaacg gacatggtcc aggccggttc gtgaggccct acgcgatgac
     2761 cggcgggcgt acccgtccct cgcatatgga cctcgagttg gagctggaga ccctggtgtc
     2821 gaccacgtcg ctcggcgagc agtcggtagc gtccctcagc ctggagcggc gctcgatcgc
     2881 gctgctctgc cgggacgtca tcagcgttgc ggaggtctcg gcccggctcg acatcccgct
     2941 cggcgtggcg cgggtcctcg tcggtgacat ggccgacgag gggctcgtga tcgtgcaccg
     3001 ccccgccagc ccaagcgacc ggcccgacct cgcacttctc gagagggtgt tgtatggact
     3061 ccggaccatc tagccgcatg ccgggcggga gctccacccc ggcgccgacc ccggtcaaaa
     3121 tcgtcatcgc cggtggcttc ggggtcggca agaccacctt cgtgggtgcc gtgtcggaga
     3181 tcatcccgct gaccaccgag gcggtcatga cctcggccag cgtcggcgtg gacgacacca
     3241 accaggtccc gggcaagacg accacgaccg tcgccatgga cttcggccgc atcacgctcg
     3301 ataccgacct gatcctctac ctgttcggca cgcccgggca ggaccggttc tggttcatgt
     3361 gggacgacct cgtccgggga gcgatcggcg ccgtcgtgct ggtcgacacc cggcggctcg
     3421 acgcctgctt cgccgccgtc gacttcttcg aacagcgggc actgccgttc atcgccgccg
     3481 tgaactgctt ccacggcgag gcgctgcacg ccgtcgacga cgtccgcgag gcgctggcga
     3541 tcgggcgtga cgtcccgatc gtgctgtgcg acgcccgggc cagggactcc gcgaaggcga
     3601 cgctcatcga gctcgtcgag catgcgctgg ccagggccac ggctgccctg gactcgtcga
     3661 gggcctgaac ccgggccgcg tcaccccgcc ggccgcccgg ccctgccacg ccggcgggac
     3721 cgccgggcga ccgcggcgcc gacgccggcc gcgagtgcgg cggcgagcag gatccccccc
     3781 aggacggccg gcccgggccg gcggcctgcc cggcccggct ggcctgcccg ggcaggccgg
     3841 ccggctgcgg ccgccga
//
LOCUS       DASPYY010000142         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1008635, whole genome shotgun sequence.
ACCESSION   DASPYY010000142 DASPYY010000000
VERSION     DASPYY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008635"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VEP73_02145"
     CDS             complement(<1..1564)
                     /locus_tag="VEP73_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HYT25275.1"
                     /translation="MQRHPGRVREATGVGGAGLALSGLAEAAGLLGVPEAAEQATEAA
                     RRLAALTLEVAVVGEFKRGKSSLINALLGREVLPVGVLPLTAVPTVLERGAPGCVVEF
                     ADGRREAHDLAQVGRFVTEEANPGNALGVARVVVRLPAPLLDQGVRLVDTPGVGSVHG
                     HNTLATGAYLPSLDAAVLVVSADPPISEAERGFLTQVVEHAVRLFVVLNKADYLAPDD
                     LARAVGFTERVVRQVLPDWPGPVYALSSRPGVGDPAGLARFREDLARFLREGRAAAAV
                     DAARRVAGRALGALRLTVALERRAAALPVEELDRARQRFDAAAGRLAEEAAADAALLT
                     AAVQRGLGALDEVVAARREALKDRAELLTVEAADRHPDLGAGRLLEVLGAERRALLER
                     VCAQTVEQAGTAAVAAYRRAAQGVAERAATRAEQLQAEAARAFGVPLPRFEPPEADLG
                     VARVSFASPRVTLLADQLAPVAWRLLGAGAARDRALAKARALAADEAEMVLGRLRGAT
                     SEQLGEAARQLGA"
     regulatory      complement(1594..1654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1739..2560
                     /locus_tag="VEP73_02150"
                     /pseudo
     CDS             1739..2560
                     /locus_tag="VEP73_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130488.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
BASE COUNT          344 a         1018 c          888 g          310 t
ORIGIN      
        1 gggcgccgag ctgccgagcg gcctccccga gctgctccga cgtcgcgccc cggagccgcc
       61 ccaggaccat ctcggcctcg tcggcggcca gcgcgcgtgc cttggcgagc gcacgatccc
      121 gggccgcccc ggcgccgagg agccgccagg ccaccggggc gagctggtcg gcgagcagtg
      181 tgacccgcgg tgaggcgaag ctcaccctgg cgacgccgag gtcggcctcg ggcggctcga
      241 accggggcag gggtaccccg aacgcccttg ccgcctccgc ctgcaactgc tcggcccggg
      301 tcgcggcccg ctcggcgacc ccctgcgcgg cgcggcggta ggccgccacc gcggccgtgc
      361 cggcctgctc gaccgtctgc gcgcagaccc gctcgagcag cgcccgccgt tccgccccga
      421 gcacctcgag caggcgcccc gcacccaggt ctggatggcg gtcggccgcc tcgacggtca
      481 gcagctcggc gcggtccttc agcgcctccc ggcgcgcggc cacgacctcg tcgagcgcgc
      541 cgagaccgcg ctgcacggcc gcggtgagca gcgcggcatc ggccgccgcc tcctcggcga
      601 gccgcccggc ggctgcgtcg aagcgctgcc tggcccggtc gagctcctcg accggcagcg
      661 ccgccgcccg ccgctccagg gcgaccgtca gccgcagtgc gcccaacgcc cggcccgcca
      721 cccgccgcgc cgcgtcgacc gccgcagctg cccgcccctc acgcaggaac cgggccaggt
      781 cctcccggaa ccgggccagc ccggccgggt cgcccacccc gggcctggac gacagcgcat
      841 acaccgggcc cggccagtcg ggcagcacct gccgcacgac ccgctcggtg aacccgaccg
      901 cgcgcgcaag atcgtccggg gcgaggtagt ccgccttgtt gagcaccacg aacaggcgca
      961 ccgcatgctc caccacctgc gtgaggaacc cccgctcggc ctcggagatc ggcgggtcgg
     1021 ccgacacgac cagcaccgcg gcgtcgaggc tgggcaggta ggcgccggtc gccagcgtgt
     1081 tgtgcccgtg caccgagccg accccgggtg tgtcgaccag gcgcaccccc tggtcgagca
     1141 gcggcgcggg cagtcgcacc accacccggg ccacgcccag ggcgttgccc gggttggcct
     1201 cctcggtcac gaaccggccc acctgcgcca ggtcatgcgc ctcgcgccgg ccgtcggcga
     1261 actcgaccac gcagccgggc gccccgcgct cgagcacggt cggcaccgcg gtcaacggca
     1321 acacgccgac cgggagcacc tcccggccga gcagcgcatt gatcaagctg gacttcccac
     1381 gcttgaactc cccgaccacc gcgacctcga gggtcagcgc ggcgagccgg cgggccgcct
     1441 cggtggcctg ctcggcggcc tctggcacgc cgagcagccc ggccgcctcc gccagccccg
     1501 acagcgccaa tccggcgccg cccacaccgg tcgcctcgcg tacccgtccc gggtgacgct
     1561 gcatcgctcc tcccctggtg catgacgatc cctgcggttg caggagccat cagccctcgg
     1621 ggcggtcaca gcgagctcca tcgctgaacg gctcagccag cgagccgcca gtaccgtagc
     1681 acccggcacg gggctgccgc gatcagcctc cgcgcaggtc gaaagcccac tgcgcggcat
     1741 caactgcggc ttcttcgccg ccgcgctggt cgcggcggcg ctggtcgcgg tggtctccct
     1801 tgtctatgtg gacgactggc gcccgctggc cgcggtcgtg ctgggcctgg tgctggccag
     1861 cggaatccag ttgatgacct cgtactacac ggccacggag tacaaaccgg tccaagagat
     1921 cgccgacgcc tcggaggccg ggccgacgac cacgatcctg tccggcttct cgaccgggct
     1981 ggagtcgacc gtgtacgcgg tgctggcgat cgtcggcgca atcatcggcg cggtcctgct
     2041 cggcgcaggc gatccgcgcg gtcggccggg ccgccggccg ggtcgtgttc gaggtccgca
     2101 agcagttccg cgaccacccg gggatcatgg actacagcga gcggcccgat tacagcgcgg
     2161 tggtggacat ctgcacccgc gactcgctac gtgagctgct gacaccgggc ctgctggccg
     2221 tgctgacgcc gatcgccacc ggcttcatcc tcaagcagga ggcgctgggc gccttcctcg
     2281 cgggggcgat cctgaccggg cagctcctgg cggtgatgct cgccaactcg ggcgcggcct
     2341 gggacaacgc caagaagtac gtggaggacg gccaccacgg gggcaagaac tccgaggcgc
     2401 acaaggcgac cgtgatcggc gacaccgtcg gcgacccctt caaggacacc gccggcccgg
     2461 ccctgaaccc gttgatcaag gtgatgaacc tggtctcggt gctgatcgcg ccgacggtgg
     2521 tggcctacgc cggcaacatc acgctccgcg tggtggtcgg
//
LOCUS       DASPYY010000143         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_587391, whole genome shotgun sequence.
ACCESSION   DASPYY010000143 DASPYY010000000
VERSION     DASPYY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587391"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /gene="cimA"
                     /locus_tag="VEP73_02155"
     CDS             <1..>1517
                     /gene="cimA"
                     /locus_tag="VEP73_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HYT25276.1"
                     /translation="GLAALTGAASRLGAASQAGAEKVEIYDTTLRDGSQQVGLDLTVA
                     DKLHVAAALDALGVDVVEGGWPGSNPKDAEFFARAAELPWRHAKLAAFGATRPPRRST
                     EDDPNLRALLAAGTPIVTLVGKAWTLHVDEALRTTREENLAMVAESVRFMAGEGRRVV
                     FDAEHFFDGYRADRGYALEVLAAAAEAGADTLALCDTNGGTLPDEAARILGEVAAATR
                     ARLGVHFHNDSACAVANSVVAVSGGALHVQGAANGYGERCGNADLFSVIASLELKRGH
                     ELLPPGRLAALASTARTIAELANLPFESRQPYVGSSAFTTKAGLHASAIARRPDAYSH
                     VDPGSVGNQARVLVSELAGRSNVLAKAEELGLDLSGDPGLAAVILDRIKDAEHRGYAF
                     EAADASFELLVRRTAGVLPAWFELEGYRVVIERGAADDRSEAIVRLLLGEHRVVAVGE
                     GVGPVHALDQALRRGLAELYPALAGIHLVDYKVRILDGRAATSAVTRVMVTSADH"
BASE COUNT          197 a          563 c          555 g          202 t
ORIGIN      
        1 ccggtctcgc cgcgctgacc ggtgccgcga gccggctcgg tgcggccagc caggcgggcg
       61 ccgagaaggt cgagatttac gacaccacgc tccgcgacgg ctcccagcag gtcggcctcg
      121 acctcaccgt cgccgacaag ctgcatgtcg cggcggcact cgacgcgctc ggcgtggacg
      181 tcgtcgaggg cggctggccg ggctccaacc cgaaggacgc cgagttcttc gcccgcgccg
      241 ccgagctgcc atggcggcat gccaagctcg cggcgttcgg cgccacccgg ccgccgcggc
      301 gcagcaccga ggatgacccg aacctccgcg cgctgctggc ggccgggacg ccgatcgtga
      361 cgctggtcgg caaggcctgg acgctgcacg tcgacgaggc gctgcgcacc acccgcgagg
      421 agaacctggc gatggtggcc gagtcggtgc gcttcatggc gggcgagggc cggcgggtgg
      481 tcttcgacgc cgagcatttc ttcgacggct accgggccga ccgcggttac gcgcttgagg
      541 tgctggccgc cgccgccgag gccggcgccg acacgctcgc gctctgcgac accaacggcg
      601 gcacgctgcc ggacgaggcc gcccgcatcc tcggcgaggt cgctgccgcc acccgggcac
      661 gcctcggcgt gcacttccac aacgactctg cctgcgcggt cgccaactcg gtggtcgccg
      721 tctccggcgg cgcgctgcac gtgcagggcg ccgccaacgg ctacggtgag cggtgcggga
      781 acgccgacct gttctcggtc atcgccagcc tcgagctgaa acgcgggcac gagctgctgc
      841 cgccgggccg gctcgcggcg ctggcttcca ctgccaggac gatcgccgag cttgccaacc
      901 tgccgttcga atcgcggcag ccctacgtcg gctcgtccgc gttcaccacc aaggcgggcc
      961 tgcacgcgtc tgccatcgcc cggcggcccg acgcctattc gcacgtcgac cccggctcgg
     1021 tcggcaacca ggcgagggtg ctggtcagcg agcttgccgg gcgcagcaac gtgctcgcga
     1081 aggccgagga gctcggcctc gacctcagcg gcgatccggg actggccgcc gtgatcctgg
     1141 accggatcaa ggacgccgag caccggggat atgcgttcga ggccgcggac gcgtcgttcg
     1201 agctgctggt gcgcaggacc gcgggcgtgc tgcccgcctg gttcgagctc gagggctacc
     1261 gcgtggtgat cgagcgcggc gccgccgatg accgcagcga ggcgatcgtc cggctgctgc
     1321 tcggcgagca ccgggtggtc gccgtcggcg agggcgtcgg gccggtgcac gcgctcgacc
     1381 aggcgctgcg ccgcggcctg gccgagctgt acccggcgct ggcgggcatc cacctggtcg
     1441 actacaaggt gcgcatcctc gatggccgcg ccgcgaccag cgcggtcacc agggtcatgg
     1501 tcacctcggc cgaccac
//
LOCUS       DASPYY010000144         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1991493, whole genome shotgun sequence.
ACCESSION   DASPYY010000144 DASPYY010000000
VERSION     DASPYY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991493"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEP73_02160"
     CDS             <1..623
                     /locus_tag="VEP73_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HYT25277.1"
                     /translation="CRFGSATLLGDLAQATTPWAAGSWEASLAHLGKPGGGVQSLPRA
                     FRAPRVVLDFANRLLPAIAPGLTAARAVREVPGALELRQVEPSRLADACARAVRDAVR
                     GEGSVGLVLADEEVDRMAAALHRHGVGHARVERLDQGSRVTLVPASQAKGLEYDQVVV
                     MEPAAIARAEPLGLRRLYIVLTRAVTHLTVVHAEPLPPLLAPPSGG"
     gene            complement(612..1580)
                     /locus_tag="VEP73_02165"
     CDS             complement(612..1580)
                     /locus_tag="VEP73_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYT25278.1"
                     /translation="MGRVGLVLGAGGVVGHAFHAGVLRALADTTGWDPRSAEVIVGTS
                     AGAHVAALLRGGFSAADVAARTAGEPVSADGARVLRRIGPNEPVPGPRSRSVGMAAPG
                     LLLRSVVRPWRAPRLGSLTAAALPRGRVSLDPFAARLRWLFDAGWPEATLWLCAVRLR
                     DGRRVVLGRPGAPTTDVGTAVAASCAIPGWFAPVRIGAAWYVDGGVHSPTNLDVLAGL
                     GLDLVVVSAPMSVARGVRRASVDLPARQALRLRLAEEARRVRRQGTQVVAFQPTAPDL
                     EVMGLNAMNPRHRYPVVRRAHESVLRRLERPDFLDRLAALAPRSPT"
BASE COUNT          207 a          653 c          649 g          164 t
ORIGIN      
        1 gctgccgctt cggctccgcg acgctcctcg gcgacctcgc gcaggcgacg accccgtggg
       61 cggccggcag ctgggaggcc agcctggcgc acctgggcaa gccaggcggg ggcgtccagt
      121 ccctgccgcg ggcgttccgg gcgccccgcg tggtgctcga cttcgccaac cggctcctgc
      181 ccgccatcgc ccccgggctc accgcggccc gggcggtgcg cgaggtccct ggcgcgctgg
      241 agctccgcca ggtcgagccg tcccggctcg ccgacgcgtg cgcgcgcgcg gtgcgtgacg
      301 ccgtccgcgg cgaggggtcg gtcggcctgg tccttgccga cgaggaggtc gaccggatgg
      361 ccgcggcgct gcaccgtcac ggggtgggcc atgcccgggt cgagcggctc gaccaagggt
      421 cgcgcgtcac cctggtgccc gccagccagg ccaaggggct cgagtacgac caggtggtcg
      481 tgatggagcc cgccgcgatc gcgcgcgccg agccgctggg cctgcggcgc ctctacatcg
      541 tgctcacgcg ggcggtcacc cacctgacgg tggtgcacgc cgagccgctc ccaccgctcc
      601 tcgcaccacc gtcaggtggg tgaccgcggc gccaaggcgg cgagccggtc caggaagtcc
      661 gggcgctcca ggcggcgcag gaccgactcg tgggcccggc ggacgaccgg gtagcggtgg
      721 cgggggttca tcgcgttcag ccccatcacc tcgaggtcgg gcgctgtcgg ctggaacgcc
      781 accacctgcg tgccctggcg gcgcacccgc ctggcctcct ccgccaggcg caggcgcagc
      841 gcctggcgcg cgggcaagtc gaccgacgcg cgccgcacgc ccctggcgac cgacatcggc
      901 gcgctgacga ccacgaggtc gaggccgagc ccggccagga cgtcgaggtt ggtaggagaa
      961 tggacgccgc cgtccacgta ccaggcggcg ccgatccgga cgggcgcgaa ccagccgggg
     1021 atggcgcagg aggcggccac cgcggtgccg acgtcggtgg tgggcgcgcc cggccggccg
     1081 agcaccacgc gccggccgtc gcgcagccgg accgcgcaga gccacagggt ggcctccggc
     1141 cacccggcgt cgaacagcca gcgcaggcgg gcggcgaacg ggtcgagcga gacccggccc
     1201 cttggcaggg cggccgccgt gagcgacccg aggcgcggcg cgcgccaggg ccggaccacc
     1261 gagcgcagca gcaggcccgg cgccgccatg ccgaccgagc ggctccgcgg gccggggacg
     1321 ggctcgttcg gcccgatcct gcgcaggacg cgggcgccat cggccgagac cggctcgcca
     1381 gccgtgcgcg cggcgacgtc ggcggcgctg aagccgccgc ggagcagcgc ggcgacgtgc
     1441 gcccccgcgg aggtcccgac gatcacctcg gccgagcggg ggtcccaccc ggtcgtgtcg
     1501 gcgagtgccc gcagcacgcc ggcgtggaag gcatgcccga cgaccccccc cgccccgaga
     1561 acgagaccga cccggcccat gcgacctccc gcctccaagt gtaggcccgg gtgggagcca
     1621 gcccctcctc ggtgaaggct ccccgggacg ctggcgcaac gaacgacggc cgc
//
LOCUS       DASPYY010000145         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2694284, whole genome shotgun sequence.
ACCESSION   DASPYY010000145 DASPYY010000000
VERSION     DASPYY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694284"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VEP73_02170"
     CDS             <1..491
                     /locus_tag="VEP73_02170"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042788.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HYT25279.1"
                     /translation="QTVDSDGLRAFVLTLQAREQHIRREKATSNICTNQTLNAIAAAV
                     YLAWLGPRGLDELGRLCLRLARYAAERLSALPGVRLAFPAQPFYKEFTVRLPADPAEV
                     CRRLAGDHGLLAGLPLGGVAPGLDDGLLVAVTERRSKADVDHLAEAMGSVLADLEKEG
                     AA"
     gene            488..2038
                     /gene="gcvPB"
                     /locus_tag="VEP73_02175"
     CDS             488..2038
                     /gene="gcvPB"
                     /locus_tag="VEP73_02175"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYT25280.1"
                     /translation="MRGHFEPAPWAAGTPGAREPERLIFERSAPGRRASSFPDGSDLP
                     EVDLEAALPAAARRSTPPRLPEVSERDLVRHYTRLAHRTYAVDLGFYPLGSCTMKYNP
                     KVAESAAALPGFARLHPAQPAEQVQGALELLGRLEHALCELTGLAGVTLQPAAGAHGE
                     LTGLLVIRAWHESRGDPRRRVIIPDSAHGTNPASVRLAGFEVTTVRSGPDGLVDLDGL
                     EAALGDDVAAFMITNPNTLGLFERNIARMAALVHGVGGQLYYDGANFNAILGKVRPGD
                     MGFDVVHLNLHKTFATPHGGGGPGAGPLVVAGHLTDFLPAPVLTRDGDAGEGGWRWDH
                     DRPRSIGRVHGWNGNFGILVRAYAYLLANGGDGLAQVAERAVLNANYLLERLSAAYQL
                     GYPTRPVMHEFVLSARRQKAAGARGLDIAKGLIDRGFHPPTVYFPLIVEEALMVEPTE
                     TEAVETLDAFADAMLDLARQAESDPQRLHEAPASAPVRRLDEARAARSLIPRWHPPEE
                     EPTESPTE"
     gene            2242..3237
                     /locus_tag="VEP73_02180"
     CDS             2242..3237
                     /locus_tag="VEP73_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYT25281.1"
                     /translation="MAGLSLLGALLLGLLFGVVPAGHADRLDTARTQVRKLRAELQAA
                     TDTEAQTEADLSQLEDAMTLDKLQLSRAKQHLGTADAQLASQAAATYQSGGGLGMLDA
                     VLNGSDQLPDRAEFLMLLLGRQNAAVDDARTARGAFNAAVGGLAADQAAAKKLHDRAK
                     AAADVMRGRFKDAQDLLGRLAGFGSGQATYPASPFGTFNGHLYACPVEPPYTYSDTWG
                     APRPGGRTHKGVDIMAPNGAKEFAYTAGVIWLEKFEPTGGNDLWIHGDDGNTYFYAHI
                     SRYALPMNTRVKAGQLIAYVGQTGDAQFTAPHLHFEVHPGGGDAVDPYPWAKRVC"
     gene            complement(3361..>3992)
                     /locus_tag="VEP73_02185"
     CDS             complement(3361..>3992)
                     /locus_tag="VEP73_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT25282.1"
                     /translation="AARGYACLLPDPALSTGYGQAFIARGRARWGQEPYSDVLALADA
                     AEARPDIDETRTAAMGGSFGGYLANWAAGHTSRFRCVVAHASVWSLEQFHGTTDDAGW
                     WDWEFGAPETEAAATRYREHSPSRHADAISVPMLVIHGERDYRVPIGEALRLWTDLQW
                     HGVEAKFLYFPDENHWILKPQHVRVWYETVLAFLDHHVLGKDWSPPKLL"
BASE COUNT          575 a         1572 c         1329 g          516 t
ORIGIN      
        1 gccagaccgt cgacagcgac ggtctgcgcg ccttcgtgct caccctgcaa gcccgcgagc
       61 agcacatccg ccgcgagaag gcgaccagca acatctgcac caaccagacc ctgaacgcca
      121 tcgccgccgc cgtctacctc gcctggctcg gcccgcgcgg cctcgacgag ctgggccgcc
      181 tgtgcctgcg cctggcccgc tacgccgccg agcggctcag cgccctgccg ggggtgcggc
      241 tcgccttccc cgcacagccc ttctataagg agttcaccgt gcgcctgccc gctgatccgg
      301 ccgaggtctg ccgccggctc gccggcgacc acggcctgct cgccggcctg ccgctcggcg
      361 gggtcgctcc cggcctcgac gacggcctgc tcgtcgccgt gaccgagcgg cgcagcaaag
      421 ccgacgtcga ccacctcgcc gaggcgatgg gcagcgtcct cgccgacctc gagaaggagg
      481 gtgcggcatg agggggcact tcgagccggc accgtgggcg gccggcaccc ccggggcgcg
      541 ggaacccgaa cggctcatct tcgagcgctc cgccccggga cggcgagcgt ccagcttccc
      601 cgacggcagc gacctacccg aggttgacct ggaggccgcc ctgccggccg ccgcccggcg
      661 cagcacgccg ccgcggctgc cggaagtctc cgagcgggac ctggtgcggc actacacccg
      721 cctcgcccac cgcacctacg cggtcgacct cggcttctac ccactcggtt cctgcaccat
      781 gaagtacaac ccgaaggtcg ccgagagcgc cgcggcgctg cccgggttcg cgcgcctgca
      841 cccggcgcaa cccgccgagc aggtgcaggg cgccctggag ctgctcggcc ggctcgaaca
      901 cgcgctgtgc gagctgaccg gccttgccgg cgtcaccctc cagccggccg ccggcgccca
      961 cggcgagctg accggcctgc tggtcatccg cgcctggcac gagtcgcgcg gcgacccgcg
     1021 ccgccgcgtg atcatccccg actccgccca cggcaccaac cccgcctcgg tgcggctggc
     1081 cggcttcgag gtcaccaccg tccgctccgg ccccgacggg ctggtcgacc tcgacggtct
     1141 ggaggcggcg ctgggcgacg acgtcgccgc cttcatgatc accaacccca acacgctcgg
     1201 cctgttcgag cgcaacatcg cccgcatggc cgcgctggtc cacggcgtcg gcggccagct
     1261 ctactacgac ggcgccaact tcaacgccat cctcggcaag gtccgccccg gcgacatggg
     1321 cttcgacgtc gtccacctca acctccacaa gaccttcgcc accccccacg gcggcggcgg
     1381 gcccggcgcc gggccgctgg tcgttgccgg ccacctcacc gacttcctgc cggcgccggt
     1441 gctcacccgc gacggcgacg ccggcgaggg cggctggcgc tgggaccacg accggccccg
     1501 ctccatcggg cgggtgcacg gctggaacgg caacttcggc atcctcgtgc gcgcctacgc
     1561 ctacctgctc gccaacggcg gcgacggcct cgcccaggtc gccgagcggg ccgtgctcaa
     1621 cgccaactac ctgctggagc ggctcagcgc cgcctaccag ctcggctacc cgacccgtcc
     1681 ggtgatgcac gagttcgtgc tgtcggcacg ccgccagaag gcagccggcg cccgcggcct
     1741 cgatatcgcc aagggtctga tcgaccgggg cttccacccg ccgacggtct acttcccgct
     1801 gatcgtcgag gaggcgctga tggtcgagcc gaccgagacc gaggcggtcg agaccctcga
     1861 cgccttcgcc gacgccatgc tcgacctcgc ccgccaggcc gagtccgacc cgcagcgcct
     1921 ccacgaagcc ccggcgagcg ccccggtccg ccgcctggac gaggcccgcg ccgcccgctc
     1981 cctcataccc cgctggcacc cgcccgagga ggagccgacg gagtcgccga cggagtagcc
     2041 gagcaggccc gaacgagtcg aagtcgttcc cgaatttgct gtcagcggcg cagcgccata
     2101 tagactaccg tcacccgagt tccccatcgg gacagtgagc ggacccgttc cccccaaacg
     2161 gaaggacgcc cagcgatgcg gcgaacctcg attcggttcg cgcaccacaa gcaaggcgag
     2221 catggacgac gccgtcctgg cttggccggc ctgtcgctgc tcggcgccct gctgctcggc
     2281 ttgctgttcg gagtcgtccc cgccgggcac gccgaccgcc tcgacaccgc ccgcacccag
     2341 gtccgcaagc tccgcgctga gctgcaggca gccactgaca ccgaggcgca gaccgaggcc
     2401 gacctcagtc agctcgagga cgccatgacc ctcgacaagc tgcagctcag ccgcgccaag
     2461 cagcacctcg gcacggccga cgcccagctc gccagccagg ccgccgcgac ctaccagtcc
     2521 ggtggcggcc tcggcatgct cgacgccgtg ctcaacggca gcgaccagct gcccgaccgg
     2581 gccgagttcc tgatgctgct gctcggccgg cagaacgccg cggtcgacga cgccaggacc
     2641 gcccgcggcg ccttcaacgc cgccgtcggc ggcctggccg ccgaccaggc tgccgccaag
     2701 aagctccacg accgcgccaa ggccgccgcc gacgtcatgc gcggccgctt caaggacgcc
     2761 caggacctgc tgggccgcct cgccgggttc ggttccggcc aggccaccta cccggcgtcg
     2821 ccgttcggca cgttcaacgg ccatctgtac gcctgcccgg tcgagccgcc ctacacctac
     2881 agtgacacct ggggagcgcc ccgccccggc ggccgcaccc acaagggcgt cgacatcatg
     2941 gcccccaacg gcgccaagga gttcgcctac accgccgggg tgatctggct ggagaagttc
     3001 gagccgaccg gcggcaacga cctgtggatc cacggcgacg acggcaacac ctacttctac
     3061 gcgcacatct cccgctacgc gctgccgatg aacacccgcg tgaaggccgg ccagctgatc
     3121 gcctacgtcg gccagaccgg cgacgcccag ttcaccgccc cgcacctgca cttcgaggtc
     3181 caccccggcg gtggcgacgc ggtcgacccc tacccgtggg ccaagcgcgt ttgttaggga
     3241 caccccgggg gcgagactgc gccgcggcgt gggtggcccg gagggggtcc atgcggctgc
     3301 ggctcgcagc tcacccctaa gggagatcgt gagtggttgg gggcgtctgt ctagagccgt
     3361 ctagagcagt ttcggggggg accagtcttt gccgaggacg tggtggtcga ggaaggcgag
     3421 gactgtctcg taccagacgc ggacgtgctg gggcttgagg atccagtgat tttcgtcggg
     3481 gaagtagagg aacttcgcct ccacgccgtg ccactggagg tcggtccata ggcgcagggc
     3541 ctcgccgatt gggacgcggt agtcgcgctc gccgtggatc accagcatcg gcacgctgat
     3601 cgcatcggcg tggcggctgg gggagtgctc gcggtagcgg gtggcggccg cctcggtctc
     3661 cggagcgccg aactcccagt cccaccaccc ggcgtcgtcg gtggtgccgt ggaactgctc
     3721 cagcgaccac acgctcgcat gcgcgaccac gcagcggaat cggctggtgt ggcccgccgc
     3781 ccagttggcc aggtagccgc cgaacgagcc gcccatcgcc gcggtgcggg tctcgtcgat
     3841 gtccgggcgg gcctcggccg cgtcggccag cgccaggaca tcgctgtagg gctcctggcc
     3901 ccagcgggcc cggccgcggg caatgaacgc ctggccgtat ccggtcgaca gggccgggtc
     3961 cggcagcagg caggcgtagc cgcgcgcggc ca
//
LOCUS       DASPYY010000146         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_868377, whole genome shotgun sequence.
ACCESSION   DASPYY010000146 DASPYY010000000
VERSION     DASPYY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868377"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VEP73_02190"
     CDS             complement(<1..827)
                     /locus_tag="VEP73_02190"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HYT25283.1"
                     /translation="MINLRYHIVSLVAVFLALAIGIVVGSTVLKEGTVGVLRTTSNQL
                     IRESKQARDENSALRGQLNAYKTFGDAVLPDLAKDRLKGRSAVLLDTDRVDDPTRNAV
                     QDAIKAAGADVDGRVTFSSDRLGLAAQGDRDSLNALLGTDLADPDTLRRSLADRLAGR
                     LTNPGRLPRGGDRARDLLTGLDDAKFLADMRLADKAMQDGTVAFPRAGSMFVIIGPTG
                     NPTPLDPLTFLVPLADRLSVRAAPVPVAGVEAAAPGGTSWVEKLRGARGVPDRVSTV"
     gene            complement(868..2052)
                     /gene="steA"
                     /locus_tag="VEP73_02195"
     CDS             complement(868..2052)
                     /gene="steA"
                     /locus_tag="VEP73_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HYT25284.1"
                     /translation="MRFLLPRRHRPEPGPGDVLGAVRRDRRTKNLVKRLRPGDVAVID
                     HADIDRVAAETLVECRPAAVVNAAASISGQYPHAGPMVLVEAGIPLLDDVGQDVFDFL
                     REGDEVRIDPDGVVYRDGSRVASGEWLRPEDVRKRVDAAKDNLATALEDFTLNTLSYV
                     RRDRDLLLEGLDIPGLATSMRDRHVLVVVRGHDYKKDLSTLRGYIREFRPVLIGVDGG
                     ADALLEEGYKPDVILGDMDSVTSEALACGAEVVVHAYPDGRAPGKERVEALGIVPFVF
                     PGAGTSEDMAMLLAYEADCDLIVAVGSHASMVEFLDKGRPGMASTFLVRLKVGSKLVD
                     AKGVNRLYRQGVRRGDLLLLVAAALVAMLVIALVSEPVRLVWEQALELLKAFFHRLTS
                     LF"
     gene            complement(2049..>3298)
                     /locus_tag="VEP73_02200"
     CDS             complement(2049..>3298)
                     /locus_tag="VEP73_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYT25285.1"
                     /translation="AVTRELDDLAARARDRAREADLLRYQLAEIEAAEVRVGESGELE
                     AEAERLANAEALRDAAASAWQALTGDDELGAATALGAAARAVAGPGRHDAALGELAER
                     AEALAAEVGDLASSLRAYTEQVLVDPERLGEVQARIALLRDLGRKYGEDEAAVLAYAD
                     EARTRLAELDGGTVRSTELEAEARHLRGRLAGVGATLSAERSAAAARLAEALQAELAD
                     LAMPHALVRVAVEHDADPDGLDVDGRRLAATADGLDRVELLLSANPGIQPRPLGRAAS
                     GGELSRVMLALRVVLAGTDKTPTLVFDEVDAGVGGRTAAAVGRRLAQLARHHQVLVVT
                     HLPQIAAFADRHFTVEKRSSEGMTSTAVRALDDAGRVTELSRMLAGMEGSGLAQAHAE
                     ELLAAASAVKGSDGARAGGGGRR"
BASE COUNT          397 a         1238 c         1245 g          418 t
ORIGIN      
        1 tcgaccgtgg acacccggtc gggcaccccc cgcgcgccgc gcagcttctc cacccaggag
       61 gtgccgccgg gggcggccgc ctcgacgccg gcgaccggga cgggcgcggc ccggaccgag
      121 agccggtcgg cgagcggcac caggaaggtg agcgggtcga gcggcgtcgg gttgccggtc
      181 ggcccgatga tcacgaacat cgacccggcc ctggggaacg cgaccgtgcc gtcctgcatc
      241 gccttgtcgg cgagccgcat gtcggcgagg aacttcgcgt cgtcgaggcc ggtcagcagg
      301 tccctcgccc ggtcgccgcc gcgcggcagg cggccggggt tggtgagccg cccggcgagc
      361 cggtcggcga ggctccgcct gagcgtgtcc gggtcggcca ggtcggtgcc cagcagcgcg
      421 ttcagcgagt cccggtcgcc ctgggcggcc agcccgagcc ggtccgacga gaacgtgacc
      481 cgcccgtcca cgtccgcgcc ggccgccttg atcgcgtcct ggacggcgtt gcgggtaggg
      541 tcgtcgaccc ggtcggtgtc cagcagcacg gcgctccggc ccttgagccg gtccttcgcg
      601 aggtcgggaa ggacggcgtc cccgaaggtc ttgtaggcgt tgagctgccc ccgcagggcg
      661 ctgttctcgt ccctggcctg cttgctctcc ctgatgagct ggttcgaggt cgtgcgcagc
      721 acccccaccg tcccctcctt gaggacggtg ctgccgacca cgatcccgat ggccagggcg
      781 aggaagaccg ccaccagcga cacgatgtgg taccgcaggt tgatcacgtg agcgtacctc
      841 cccgggcggc cggggccgcc cggtgtgcta gaacagggac gtgagccggt ggaagaacgc
      901 cttcagcagc tcgagcgcct gctcccagac caggcggacg ggctcggaga cgagcgcgat
      961 cacgagcatg gcgaccagcg ccgccgccac cagcagcagc aggtcgccgc ggcgcacccc
     1021 ctgccggtag aggcggttca cccccttggc gtccaccagc ttcgagccca ccttgaggcg
     1081 gacgaggaac gtcgaggcca tccccggccg ccccttgtcc aggaactcga ccatgctggc
     1141 gtggctgccg accgcgacga tcaggtcgca gtccgcctcg taggcgagca gcatcgccat
     1201 gtcctcgctg gtcccggcgc cggggaagac gaacgggacg atcccgagcg cctcgacccg
     1261 ttccttgccc ggcgcgcggc cgtccgggta ggcgtgcacc accacctcgg cgccgcaggc
     1321 gagggcctcg ctggtcaccg agtccatgtc cccgaggatg acgtcgggct tgtagccctc
     1381 ctcgagcagc gcgtcggccc cgccgtcgac cccgatcagc accgggcgga actcgcggat
     1441 gtagccccgc agggtcgaga ggtccttctt gtagtcgtgc ccgcggacga ccaccagcac
     1501 gtgccggtcg cgcatggagg tcgcgaggcc ggggatgtcg agcccctcca gcagcaggtc
     1561 gcggtcccgg cgcacgtagg agagcgtgtt gagggtgaag tcctcgagcg cggtcgccag
     1621 gttgtccttg gcggcgtcga cccgcttgcg gacgtcctcg ggccgcagcc actcgccgga
     1681 tgcgacccgg ctgccgtcgc ggtagacgac gccgtcgggg tcgatgcgga cctcgtcgcc
     1741 ctcgcgcagg aagtcgaaca cgtcctggcc gacgtcgtcg aggagcggga tgccggcctc
     1801 gacgaggacc atcgggccgg cgtgggggta ctggcccgag atgctcgcgg ccgcgttgac
     1861 cacggcggcg gggcggcact cgaccagggt ctcggccgcg acccggtcga tgtcggcgtg
     1921 gtcgatcacc gccacgtcgc ccgggcgcag ccgcttgacc aggttcttgg tgcgccggtc
     1981 cctgcggacc gccccgagga cgtccccggg gcccggctcg ggccggtgcc gacgcggcag
     2041 gaggaacctc atcgacgccc cccgccgccg gctcgcgcac cgtcggagcc cttgaccgcc
     2101 gaggccgcgg cgagcagctc ctcggcgtgc gcctgcgcga gcccggaccc ctccatcccg
     2161 gccagcatcc gcgacagctc ggtcacccgg ccggcgtcgt cgagcgcccg gaccgcggtg
     2221 ctggtcatgc cctcggagga gcgcttctcg accgtgaagt gccggtcggc gaacgcggcg
     2281 atctgcggca ggtgggtgac cacgagcacc tggtggtggc gggcgagctg ggcgagccgg
     2341 cgcccgacgg cggcggcggt gcggccgccg acgcccgcgt cgacctcgtc gaagacgagc
     2401 gtcggggtct tgtcggtgcc ggccaggacc acccgcagcg cgagcatgac ccgcgacagc
     2461 tcgccgccgg acgcggcccg gccgagcggg cgcggctgga tgccggggtt cgccgacagg
     2521 agcagctcga cccggtcgag cccgtcggcg gtggccgcga gccgcctgcc gtcgacgtcg
     2581 agaccgtccg ggtcggcgtc gtgctcgacg gcgacccgga ccagcgcgtg cggcatcgcg
     2641 aggtcggcga gctcggcctg cagcgcctcg gccagccgcg cggccgccgc cgaccgctcg
     2701 gccgagagcg tcgccccgac cccggccagg cggccgcgga ggtggcgggc ctcggcctcg
     2761 agctcggtgg agcgcaccgt cccgccgtcg agctccgcga gcctggtcct ggcctcgtcc
     2821 gcgtaggcga gcacggcggc ctcgtcctcg ccgtacttgc ggccgaggtc gcggagcagc
     2881 gcgatcctgg cctggacctc gccgaggcgc tcggggtcga cgagcacctg ctcggtgtag
     2941 gcgcgcaggc tggacgcgag gtcgcccacc tcggcggcga gcgcctcggc ccgctcggcc
     3001 agctccccga gcgcggcgtc gtgccggccc ggcccggcga cggcccgggc ggcggcgccg
     3061 agggcggtgg ccgcgcccag ctcgtcgtcg ccggtcagcg cctgccaggc cgaggcggcg
     3121 gcgtcgcgca gggcctcggc gttcgcgagg cgctcggcct cggcctcgag ctcgccggac
     3181 tcgcccaccc gcacctcggc cgcctcgatc tcggcgagct ggtagcgcag caggtcggcc
     3241 tcgcgggcac ggtcgcgggc acgggcggcg aggtcgtcga gctccctggt gaccgccc
//
LOCUS       DASPYY010000147         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1711348, whole genome shotgun sequence.
ACCESSION   DASPYY010000147 DASPYY010000000
VERSION     DASPYY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711348"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VEP73_02205"
     CDS             complement(<1..196)
                     /locus_tag="VEP73_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYT25286.1"
                     /translation="MLVDTHCHLHLLDGAAEDIVGQARTEGVGHVVDVGIDLASSREA
                     AGNAARIPAVSATAGIHPNEA"
     gene            complement(233..778)
                     /locus_tag="VEP73_02210"
     CDS             complement(233..778)
                     /locus_tag="VEP73_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HYT25287.1"
                     /translation="MAPHDPQPVELVEYDPSWPKQFAEERDRIQAALGHAALVVEHIG
                     STAVPGLPAKPIIDIMVGVSDIERSGQAVAALIDLGYDYAPEFETDIPERRYFYRGIP
                     HSHHVHMVARTSDFFDRHLIFRDHLRTHPEAAAEYARLKRGLAARFRNDRDAYTEGKR
                     AFVETVVEAALRARGDRRPDR"
     gene            complement(783..2360)
                     /gene="metG"
                     /locus_tag="VEP73_02215"
     CDS             complement(783..2360)
                     /gene="metG"
                     /locus_tag="VEP73_02215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYT25288.1"
                     /translation="MKDTFYITTPIYYINDEPHIGHAYTTVAADVLARWRRAHGERVF
                     FLTGTDEHGQKVARAAEARGLDPQTWTDQIVERWKAAWADLDVSYDDFIRTTEERHVL
                     PVQHLWQRLHDQGDVYLDSYEGLYCVSCEQFYSPDELVDGRCPIHGRPVELVREENYF
                     FRLSRYADRLLALYREHPDFVRPAARLHEVRSFVEQGLQDLSISRSSFDWGVPVPWDP
                     KHVIYVWIDALQNYATAAGWDADLERFERVWPADYHFVGKDILRFHAVIWPAILMAAG
                     LPVPRTVQAHGWLLVGGEKMSKTKLTGIAPRDLVGPFGSDAVRYFFQREVAFGQDGNF
                     SWEAMVERYNADLANGLGNLASRVTAMVERYRDGVLPGPGPAGDAEVAVQEAAARAYA
                     DAKAALHGDLAYERALAGIWRLVGAANGYLSDRKPWVLAKSGDDLALDTVLYTAAEAL
                     RVIALLTAPWLTKAAPAIWAAVGAPGELADARLPEALVWGGLPRGARVTRVAALFPRL
                     DAEGRVAGTAGQSAAVG"
     gene            complement(2357..3307)
                     /gene="rsmI"
                     /locus_tag="VEP73_02220"
     CDS             complement(2357..3307)
                     /gene="rsmI"
                     /locus_tag="VEP73_02220"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYT25289.1"
                     /translation="MTGQAGRLVVCGTPIGNLGDVSPRLAAALADADLVACEDTRQTG
                     KLLAHLGVRARTVSYHEANERSRAAELAGEVAAGKLVALVSDAGMPGVSDPGHDLIGA
                     CLARGLPVEVVPGPSAVLAALVLSGLPADRFCFEGFLPRRPGPRDRRLAELAGERRTM
                     VFFEAPHRVLATLAAMAEAFGPGRPAAVARELTKLHEEVLRGPLAELAARLEAQGPRG
                     ELTLVVGGAPERAADAAGLVAEVERLVGEGATTKDAVGAVATRAGAPRRALYQAVLDA
                     RSAAPAPARRPGASLPTGGSLAGTVLPAPPSRSSGSLESP"
     gene            complement(3407..>3831)
                     /locus_tag="VEP73_02225"
     CDS             complement(3407..>3831)
                     /locus_tag="VEP73_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HYT25290.1"
                     /translation="SLHAGLSTYGDPAVDRAFVPPEPYRVPPATARAVNHRRALGGRV
                     IAVGTTAVRALETAALDGEVAARSGVTRLRIMPSSRLQVVDGLLTGFHEPEASHLDML
                     GAFVSPETLRTAYDAAIVAGYLWHEFGDVCLLRPARPA"
BASE COUNT          485 a         1378 c         1472 g          496 t
ORIGIN      
        1 ccgcctcgtt ggggtggatc cccgccgtgg ccgagaccgc cgggatccgg gccgcgttgc
       61 cggcggcctc gcggctcgag gccaggtcga tgccgacgtc gaccacgtgg ccgacgccct
      121 cggtcctggc ctggccgacg atgtcctcgg cggccccgtc gagcaggtgg agatggcagt
      181 gggtgtcgac cagcacggtg gagcggtccc ctcgtcgtcg gcgtcctgac tgtcaccggt
      241 ccgggcgccg gtcccctcgt gcccgcaggg cggcctcgac gaccgtctcc acgaaggcgc
      301 gcttgccctc ggtgtaggcg tcgcggtcgt tgcggaaccg ggccgcgagc ccccgcttga
      361 gccgcgcgta ctccgcggcc gcctcgggat gggttcgcag gtggtcgcgg aagatgaggt
      421 gccggtcgaa gaagtcgctc gtccgcgcca ccatgtggac gtggtggctg tgggggatgc
      481 cccggtagaa gtagcgccgc tccgggatgt cggtctcgaa ctcgggggcg tagtcgtagc
      541 cgaggtcgat cagggcggcg accgcctggc cggagcgctc gatgtcgctc acgccgacca
      601 tgatgtcgat gatcggcttg gcggggaggc cgggcacggc ggtcgagccg atgtgctcga
      661 cgaccagggc ggcgtggccg agggcggcct ggatccggtc ccgctcctcg gcgaactgct
      721 tcggccagga cgggtcgtac tcgacgagct cgaccggctg ggggtcgtgc ggtgccatcc
      781 cgctacccca ccgccgcgga ctgccccgcc gtcccggcca ccctgccctc ggcgtcgagc
      841 ctcgggaaca gggcagcgac cctggtcacc cgagcccccc gcgggagccc gccccagacg
      901 agcgcctcgg gcagcctggc gtcggccagc tcgccgggcg caccgaccgc ggcccagatc
      961 gccggggccg ccttcgtcag ccagggcgcg gtgaggagcg cgatgacgcg cagcgcctcg
     1021 gccgccgtgt acaggaccgt gtcgagggcc aggtcgtcgc ccgacttggc cagcacccac
     1081 ggcttgcggt cggagaggta gccgttggcc gcgccgacga ggcgccagat gccggccagg
     1141 gcgcgctcgt aggcgaggtc gccgtgcagg gccgccttgg cgtcggcgta ggcacgggcc
     1201 gcggcctcct gtacggcgac ctcggcgtcg ccggccggcc cggggccggg cagcaccccg
     1261 tcccggtacc gttcgaccat cgcggtgacc cggctggcca ggttgccgag gccgttggcg
     1321 aggtcggcgt tgtaccgctc gaccatcgcc tcccaggaga agttgccgtc ctggccgaac
     1381 gcgacctcgc gctggaagaa gtagcggacc gcgtcggagc cgaacgggcc gacgaggtcc
     1441 cggggcgcga tcccggtcag cttggtcttg ctcatcttct cgccgccgac caggagccag
     1501 ccgtgcgcct ggaccgtccg gggcacgggc aggccggccg ccatgaggat ggcgggccag
     1561 atgaccgcgt ggaagcggag gatgtccttg ccgacgaagt ggtagtcggc gggccagacc
     1621 cgctcgaagc gctcgaggtc ggcgtcccag ccggccgcgg tcgcgtagtt ctgcagcgcg
     1681 tcgatccaca cgtagatgac gtgcttcggg tcccacggga cgggcacgcc ccagtcgaag
     1741 ctcgagcgcg agatcgacag gtcctgcagc ccctgctcca cgaacgagcg gacctcgtgg
     1801 agccgggccg cggggcggac gaagtccggg tgctcccggt agagcgcgag cagccggtcg
     1861 gcgtaccggg agaggcggaa gaagtagttc tcctcgcgga ccagctcgac cgggcggccg
     1921 tggatcgggc acctgccgtc gaccagctcg tccggcgagt agaactgctc gcaggacacg
     1981 cagtacagcc cctcgtagct gtcgaggtag acgtcgccct ggtcgtggag ccgctgccac
     2041 aggtgctgga cgggcaggac gtggcgctcc tcggtcgtgc ggatgaagtc gtcgtaggag
     2101 acatccaggt cggcccaggc cgctttccag cgctcgacga tctggtcggt ccaggtctgg
     2161 gggtcgaggc ccctggcctc ggccgccctg gcgaccttct ggccgtgctc gtcggtgccg
     2221 gtgaggaaga agacgcgctc cccgtgggca cgccgccagc gggccagcac gtcggcggcc
     2281 acggtggtgt aggcgtgccc gatgtggggc tcgtcgttga tgtagtagat cggggtggtg
     2341 atatagaagg tgtccttcac ggcgactcca aggacccgga cgatctcgac ggaggtgcgg
     2401 gcagtaccgt accagctaac gatcccccgg tcggcaggct cgcgccgggc cggcgcgctg
     2461 gcgcaggcgc ggcggaccgg gcgtcgagca ccgcctggta gagggcccgg cgcggcgcgc
     2521 ctgcccgcgt ggccaccgcc ccgaccgcgt ccttggtggt cgcgccctcg ccgaccagcc
     2581 gctcgacctc ggcgacgagg ccggccgcgt ccgcggcgcg ctccggggcg ccgccgacga
     2641 cgagcgtgag ctcgccccgg ggaccctgtg cctcgagccg cgcggcgagc tccgcgagcg
     2701 ggccgcgcag tacctcctcg tgcagcttgg tcagctcccg ggccaccgca gcgggacggc
     2761 cggggccgaa cgcctccgcc atcgcggcga gcgtggcgag cacccggtgg ggcgcctcga
     2821 agaacaccat cgtccggcgc tcgccggcca gctcggcgag gcgccggtcg cgaggcccgg
     2881 gccggcgcgg caggaagccc tcgaagcaga agcggtcggc cggcagcccg ctcagcacga
     2941 gcgccgccag gaccgcgctc ggaccgggga ccacctcgac cggcaggccg cgggcgaggc
     3001 aggcgccgat caggtcgtgg ccggggtcgg acacgcccgg catgccggcg tcgctgacca
     3061 gggcgaccag cttccccgcc gcgacctcac cggccagctc ggccgcccgg ctgcgctcgt
     3121 tcgcctcgtg gtagctcacc gtgcgcgccc tcacgccgag gtgggcgagg agcttgccgg
     3181 tctgcctggt gtcctcgcag gccaccaggt cggcgtcggc cagggccgcg gcgaggcgcg
     3241 gcgagacgtc gccgaggttg ccgatcggcg tcccgcagac caccagccgg cctgcctgcc
     3301 cggtcatcga agctcctctc gccagtgcgg atgcgccagt gtgatgcgcc cggtccgccg
     3361 cccccgggcc gccgccccag tccgccatgg tacgaggacg ggcgcctcag gcgggccggg
     3421 ccggccgcag caggcagacg tcgccgaact cgtgccacag gtagccggcc acgatagcgg
     3481 cgtcgtaggc cgtccggagc gtctcggggg acacgaaggc gccgagcatg tcgaggtggc
     3541 tcgcctccgg ctcgtggaac ccggtcagca ggccgtcgac gacctggagg cggctgctcg
     3601 gcatgatgcg caggcgggtg acccccgacc gggcggccac ctccccgtcc agcgcggccg
     3661 tctccagcgc cctgacggcg gtggtgccga ccgcgatcac gcggccgccc agggcccggc
     3721 ggtggttgac cgcccgcgcg gtggccgggg gcacccggta cggctcgggg gggacgaacg
     3781 cgcggtcgac ggcggggtcg ccgtaggtgg acagcccggc gtgcaggctg a
//
LOCUS       DASPYY010000148         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2554307, whole genome shotgun sequence.
ACCESSION   DASPYY010000148 DASPYY010000000
VERSION     DASPYY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554307"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VEP73_02230"
     CDS             <1..468
                     /locus_tag="VEP73_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYT25291.1"
                     /translation="GLKVETVEDVGEVRYGTWTGRELKELAKEDLWRTVQRYPSGARF
                     PDGESIYEMQVRAVAALERLRAGHPDQTVAVCSHADVIKAVAAHYLGLHLDLFQRLEI
                     SPAGLTAFAFTPVPHLLRLNGTGGDADLKRPERPKDPEAGAARGGEGAGDAQS"
     gene            455..1006
                     /locus_tag="VEP73_02235"
     CDS             455..1006
                     /locus_tag="VEP73_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HYT25292.1"
                     /translation="MPSRDLDPVTRLTADTVGVPGRRSFFLQAASGADQVTLLLEKEQ
                     VRALAERLQSLLPEMAAEHPEDPDEARAAEAGELSLMEPLEPDFRVGQLSLDYDSGRD
                     RFVMVATELTAAEADEDTAPPEPPEPREVRLWVTRPQLRVLARHGAQVVARGRPPCPL
                     CGNPLDPTGHVCPALNGHRPTVA"
     gene            1003..1758
                     /locus_tag="VEP73_02240"
     CDS             1003..1758
                     /locus_tag="VEP73_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HYT25293.1"
                     /translation="MSESARSGPDPLPATVVPEAHARELLTEGEIEVLGRIPWASNAT
                     LLAEVSHGGLNGLGIYKPARGERPLWDFPTGTLYRREVAAYLVSEALGWGLVPLTVVR
                     EGPFGVGSVQLYVEADPDVTAFDLLAAEHPSLPRIAAFDVVVNNADRKAGHCLHALDG
                     RVWAIDHGVCFHTEPKLRTVLWDLAGQPLGSDAVADLERLSATIRGEPDGRLGALLEP
                     GELEAVARRAARLSRTAALPSPGPGRPYPWPLV"
BASE COUNT          286 a          789 c          769 g          250 t
ORIGIN      
        1 gggctcaagg tggagaccgt ggaggacgtc ggcgaggtcc gctacgggac ctggaccggc
       61 cgggagctca aggaactcgc caaggaggac ctctggagga ccgtccagcg ctacccgtcg
      121 ggtgcgcgct tcccggacgg cgagtcgatc tacgagatgc aggtgcgggc ggtggccgcg
      181 ctggagcggc tgcgggccgg gcacccggac cagacggtcg cggtgtgcag ccacgccgac
      241 gtgatcaagg cggtcgcggc ccactacctc ggcctccacc tcgacctctt ccagcgcctc
      301 gagatcagcc cggccggcct gaccgcgttc gcgttcaccc cggtgcccca cctgctgcgg
      361 ctcaacggga ccgggggcga cgcggacctg aagcggccgg agcggccgaa ggaccccgag
      421 gcgggcgccg ccaggggcgg agagggggcg ggcgatgccc agtcgtgacc tcgacccggt
      481 cacccggctc accgccgaca ccgtcggcgt ccccggccgg cgcagcttct tcctgcaggc
      541 cgcgtcaggc gccgaccagg tcaccctgct gctcgagaag gagcaggtgc gggccctcgc
      601 cgaacgcctg cagagcctcc tgcccgagat ggccgccgag caccccgagg acccggacga
      661 ggcgagagcg gccgaggcgg gcgagctgtc gctgatggag ccgctcgagc cggacttccg
      721 ggtgggccag ctctcgctcg actacgactc gggccgtgac cggttcgtca tggtcgccac
      781 cgagctgacc gccgcggagg ccgacgagga caccgcgccg cccgagccgc ccgagccgag
      841 ggaggtgcgc ctgtgggtga cccgccccca gctccgcgtc ctcgccaggc acggcgccca
      901 ggtggtggcg aggggccgcc cgccctgccc gctgtgcggg aaccccctcg acccgaccgg
      961 ccacgtctgc cccgcgctga acgggcaccg gcccacggtc gcatgagcga gtcggcgcgg
     1021 tcgggcccgg accccctccc cgcgacggtg gtgcccgagg cccacgcccg ggagctgctc
     1081 accgaggggg agatcgaggt gctcgggcgc atcccctggg cttcgaacgc caccctcctc
     1141 gccgaggtca gccacggcgg cctgaacggg ctcggcatct acaagccggc cagaggcgag
     1201 cggccgctgt gggacttccc caccgggacc ctgtaccggc gggaggtggc ggcgtacctc
     1261 gtcagcgagg cgctcggctg ggggctcgtc ccgctcacgg tcgtgcgcga ggggccgttc
     1321 ggggtcggct cggtccagct ctacgtcgag gccgaccccg acgtcaccgc gttcgacctg
     1381 ctcgcggccg agcacccgag cctgccccgg atcgccgcct tcgacgtcgt cgtcaacaac
     1441 gccgaccgca aggccggcca ctgcctgcac gccctcgacg gccgggtgtg ggccatcgac
     1501 cacggcgtct gcttccacac cgagccgaag ctgcggaccg tcctgtggga cctcgccggc
     1561 cagccgctcg ggtcggacgc ggtggccgac ctcgagcggc tctccgcgac gatccggggc
     1621 gagccggacg gccggctcgg cgcgctgctc gagcccggcg agctcgaggc cgtcgcgagg
     1681 cgggcggcgc gcctgtcccg gacggcggcg ctgccctcgc cgggccccgg ccggccctac
     1741 ccctggccgc tcgtctgaac acgaccgttg cgcttccgcg caatgatggt tgaagccggt
     1801 ttggcctgag aaaactctta cagacgtcga caaggtggtt gaagtacgat cgccagaacc
     1861 gcgcggcgaa acgccggtcg tggcccgtag cgccaggccc gcccccggcc cacggtgcga
     1921 tgtcggcgcc gaccagtgtc ggcatgggag gtgcacatgt ctcaggtcct gcctggcacg
     1981 gaggggaccg tcgaggggca ggcgacgcag gtcccggccg ccccgggcgc acacgaagag
     2041 ccgatcaagc ggatcgaggg ccgcaccccg acccagctcg ccctggccag gctg
//
LOCUS       DASPYY010000149         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_727976, whole genome shotgun sequence.
ACCESSION   DASPYY010000149 DASPYY010000000
VERSION     DASPYY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727976"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VEP73_02245"
     CDS             complement(<1..727)
                     /locus_tag="VEP73_02245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT25294.1"
                     /translation="MDGIDPRAADLTAPRGPAPPAGAYGRYAEVYDRVWARAPYGRFV
                     DLCLEEARSPVRRVVVAACGTGNAAFEFARRGYRTAGFDLSSTMLAAAAAKRPPGASA
                     DFVRGDLRAVPFADGCADLVVALNSGLNYLLEADEAVTALAHLGRVAGPAGTVVVEPL
                     SARFLHAGVEQNRHLEERGLRFDAVYETRGDLLVECVSWRVDGDKLRETYWQRWYDDD
                     RLAGMFEAAGLKLAGRRPMWPAIP"
     gene            complement(896..1186)
                     /locus_tag="VEP73_02250"
     CDS             complement(896..1186)
                     /locus_tag="VEP73_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25295.1"
                     /translation="MGTKTITMYVSDIDGDEIPAGSAYQLSVRHPDGSTQQLDISEKN
                     YRDLKLEGLGKTLKKRGRKPGSSTNKAAAGAGTTRRRSSRSANGNATQATTA"
BASE COUNT          206 a          579 c          622 g          238 t
ORIGIN      
        1 cggggatcgc gggccacatc ggccggcgac cggccaactt caggccggcc gcctcgaaca
       61 tgccggcgag ccggtcgtcg tcgtaccacc gctgccagta ggtctcgcgc agcttgtcgc
      121 cgtccacccg ccagctcacg cactcgacga gcaggtcgcc cctggtctcg tagacggcgt
      181 cgaaccgcag cccgcgctcc tcgaggtgcc ggttctgctc gacgcccgcg tggaggaacc
      241 tcgccgacag cggctcgacg acgacggtcc cggccgggcc ggcgacgcgg ccgaggtgcg
      301 ccagcgccgt cacggcctcg tccgcctcca gcaggtagtt gagccctgag ttgagtgcca
      361 cgacaaggtc ggcgcagccg tcggcgaacg ggaccgcccg caggtcgccg cggacgaagt
      421 cggcggaggc gccgggcggg cgcttggcgg ccgccgccgc gagcatggtg gacgacaggt
      481 cgaagccggc ggtccggtac ccgcggcgcg cgaactcgaa ggcggcgttg ccggtcccgc
      541 aggcggccac gacgacccgg cggacggggc tcctggcctc ctcgaggcag aggtcaacga
      601 agcgcccata gggtgcgcgc gcccacaccc ggtcgtacac ctccgcgtag cggccgtagg
      661 cccctgccgg cggagccggt ccacgggggg cggtcaggtc cgcggcccgc ggatcgatcc
      721 cgtccatgca ccagcagtat cacctcgcgt gcttgcggtg cgacctggtg cggggacctg
      781 gggcacgtgg cgccaccggc acgcgggctc gtggttcgcc cgcgtgccgg tgacgcgtgc
      841 cggtgcgctc ccgggccgcc ccggtgccgg atgcgcggcg gcccgggagg cgtcgtcagg
      901 ccgtcgtggc ctgcgtggcg ttgccgttgg cggagcggga ggagcgcctg cgcgtggtcc
      961 cggcgccggc ggctgctttg ttggtggagc tgccgggctt gcggccacgc tttttcaggg
     1021 tcttgcccag gccctcgagc ttgaggtccc tatagttctt ctcggagata tcgagctgct
     1081 gcgtcgagcc gtcgggatgg cggacggaga gctgataggc ggacccggca ggaatctcat
     1141 caccgtcaat gtccgaaacg tacatggtga tggtcttggt gcccatcgga ctcctttcac
     1201 ggcgtgtcag cgcaacttca aagggaagct gcccagattt ggggcaagcc tagccaccac
     1261 cggcctgttg tgcaacatcc atcgactttt tgttgggagg ttattgctcc ggaggcgctg
     1321 ggaccgggtc cgcaggggcc gctcgggtcg tcgcgcaacc ggcgggcgac gctcgaggcg
     1381 ccgtgctcga gagcaaacct ggccggtgtc gtcaggggcc gggcggggga gaaaccgctc
     1441 gcgtgcggtg gtcgccggcc gccgccccgg tcgcgcgaaa gccatgcggc gcaagcgaat
     1501 caggaaatcg agcgggcgcc ggccgcgcct tcagccggcg cccgccggcc gcgcccgtcg
     1561 tcctgcccgg ccggcgccgg cggctgcgtt gccgggcgcc ggcgggctgc gttgccgggg
     1621 atgcggccgc cccgccttcc gctcg
//
LOCUS       DASPYY010000150         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_869435, whole genome shotgun sequence.
ACCESSION   DASPYY010000150 DASPYY010000000
VERSION     DASPYY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869435"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="argF"
                     /locus_tag="VEP73_02255"
     CDS             <1..801
                     /gene="argF"
                     /locus_tag="VEP73_02255"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYT25296.1"
                     /translation="VAVLFDKPSLRTRASFTVGIAELGGLPMVIDTQTTHFGRGEPVE
                     DVARVLSRQVAAVAWRTYEQYRIEALAAVSAVPVINALTDQFHPCQVLADLQTVRAHY
                     GSLAGRVLTFLGDGSSNMSQSYLLGGATAGMRVRIGAPGTLVPDPAVIAAATRIAGQT
                     GGGVEIVTDPAAACEQADVIATDVWASMGQEAMAGERRVVLEPYRLDGAKLALGKPGC
                     LVLHCLPAHRGEEISADVIDGPASVVWEQAENRLHAQKALLTWLVEQS"
     gene            798..1391
                     /locus_tag="VEP73_02260"
     CDS             798..1391
                     /locus_tag="VEP73_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HYT25297.1"
                     /translation="MSTPLTKTARHARIADILAREQVRSQEELAELLERYASVHVTQA
                     TLSRDLDELGVVRLRQGGSLVYALPEEPGGPGSHPGGPGSHTSGARTETPHDIRLARY
                     LGELMTSAEASANLVVLRTPAGAAQFLASVIDHAALPAVLGTVAGDDTVLVIARDPAG
                     GDVLAADFLRRANRRRLPPRPALTGLRPGPRPAAGGN"
BASE COUNT          203 a          593 c          589 g          195 t
ORIGIN      
        1 gtggccgtcc tgttcgacaa gccgtcgctg cgcacccggg catcgttcac cgtcggcatc
       61 gccgagctcg gcgggctccc gatggtcatc gacacgcaga ccacgcactt cggccggggg
      121 gagccggtcg aggacgtggc ccgggtcctg tcccggcagg tcgcggccgt cgcctggcgg
      181 acctacgagc agtaccgcat cgaggccctg gccgccgtct cggccgtgcc ggtgatcaac
      241 gccctcaccg accagttcca cccctgccag gtcctcgccg acctgcagac cgtccgcgcc
      301 cactacggct cgctggccgg ccgcgtgctg accttcctcg gcgacggctc gagcaacatg
      361 tcgcagtcgt acctgctcgg cggggcgacc gccgggatgc gggtgcgcat cggcgctccc
      421 gggaccctgg tgcccgaccc ggcggtcatc gccgcggcca cgcggatcgc cgggcagacc
      481 gggggcgggg tcgagatcgt caccgacccc gcggccgcgt gcgagcaggc cgacgtgatc
      541 gccaccgacg tgtgggcctc catgggccag gaagccatgg ccggggaacg gcgcgtggtc
      601 ctcgagccgt accgcctgga cggcgcgaag ctggcgctgg gcaagccggg ctgcctggtg
      661 ctgcactgcc tgcccgcgca ccgcggcgag gagatcagcg ccgacgtgat cgacggcccg
      721 gccagcgtgg tctgggagca ggcggagaac cgcctgcacg cccagaaggc actgctcacg
      781 tggttggtcg aacagtcgtg agcacgccgc tgaccaagac ggccaggcac gcgcggatcg
      841 ccgacatcct ggcccgcgag caggtccgct cccaggagga gctggccgag ctgctcgagc
      901 ggtacgccag cgtgcacgtg acccaggcga ccctgtcccg ggacctggac gagctgggcg
      961 tggtccggct gcgtcagggc ggctccctgg tgtacgcgct gcccgaggag ccgggcggcc
     1021 ccggctcgca ccccggcggc cccggctcgc acacgagcgg cgccaggacc gagaccccgc
     1081 acgacatcag gctggcccgc tacctgggcg agctgatgac ctcggccgag gcgagcgcca
     1141 acctggtcgt gctgcggacg ccggccggcg cggcccagtt cctcgcctcg gtcatcgacc
     1201 acgcggccct gccggccgtc ctgggcaccg tggccggcga cgacaccgtc ctcgtcatcg
     1261 cgcgggaccc ggccggcggc gacgtcctgg ccgccgactt cctccgccgc gccaaccgcc
     1321 gccgattacc gccccgcccg gctctcaccg ggctccgccc cggcccacgc ccggcggcgg
     1381 gcggaaactg actcgcggcg gccggccgaa actgaggcgt tcgtgcggta gaaagaggga
     1441 ttgtggcagc gggacagggt gcgggtacgg gtgacgaggg cacggcgacg acgcggctgt
     1501 ggggcgggcg gttcggcgcc gggccggccg acgcgctggc gcggctgtcg gtcagcgtcc
     1561 agttcgactg gcggctggcg
//
LOCUS       DASPYY010000151         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2274794, whole genome shotgun sequence.
ACCESSION   DASPYY010000151 DASPYY010000000
VERSION     DASPYY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274794"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..151)
                     /locus_tag="VEP73_02265"
                     /pseudo
     CDS             complement(1..151)
                     /locus_tag="VEP73_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
     gene            complement(202..1215)
                     /locus_tag="VEP73_02270"
                     /pseudo
     CDS             complement(202..1215)
                     /locus_tag="VEP73_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1272..2105)
                     /locus_tag="VEP73_02275"
     CDS             complement(1272..2105)
                     /locus_tag="VEP73_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25298.1"
                     /translation="MFLRAAGQVVRLLAGEPARRWRVRSAGSDAATAHHDLTLAHAAL
                     ASNWREALAAAGHFAAVLRDVTVALANRVWAWLLATTARARRQAGPAAETAKRSIAAG
                     LTDATRASRTGLSRVGSRVGAALLAAGSAAAARIDRMVERIRVERAADKRASRPGPGA
                     GQSAETEVEGASASTTGIRGRSGRILSVLGGDHIDPAGNSDSTQQMPAAEPTWEEEDN
                     FWWLPSRQAEQAEWHAEPQEVEHQRWDEPPSPSQPGEPRETSEARGATSTDDPPSATL
                     G"
     gene            complement(2263..3240)
                     /locus_tag="VEP73_02280"
     CDS             complement(2263..3240)
                     /locus_tag="VEP73_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYT25299.1"
                     /translation="MATILVLVEHEEGNLKKVSAEMLAKARELGEPAAVFVGEGLDSA
                     RDGLARHGAATIYAATGEGWSNQLPGPLTDALDAAASRAEPAAVLVAATPAGKDAGAR
                     LAARKGWGMLADVSDIDSATGSGDGGGLVATQNVFGGATIVRARVTTGVPVLAVKPNS
                     FPAQEAPAEPQVVDLGQREATGAEIRVIDHVVEERGGRPDVAEAETIVSGGRGLQDPA
                     NFALVEALADQLGAAVGASRAAVDAGWYPHQHQVGQTGKTVSPQLYVAVGISGAIQHR
                     AGMQTSKTIVAINKDADAPIFAIADFGLVGDLFKVVPRLTDEIAKRKGG"
     gene            complement(3270..4058)
                     /locus_tag="VEP73_02285"
     CDS             complement(3270..4058)
                     /locus_tag="VEP73_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYT25300.1"
                     /translation="MKILVTVKRVPDTAAEKRLDPADYTLDRESVETIMNPVDEYAVE
                     EALRLKESSGEGEVVVLCMGPESALPAIRKALSLGPDAAVHVHDDALHGSDALATAKV
                     LAAAIRHVGPDLVLSGSESTDARGSLVPSAVAELLGWPGLIGAKQLEVEGATVRIHRE
                     TEAGYDVLEAQGPAIVSVVKGINEPRYPSFRGIMAAKSRPVTDLTIADLGIDPGEVGS
                     AGATTAVVDATSRPPRQSGQVVKDPGDGAAATELVEFLAAHKFI"
     gene            4262..>4497
                     /locus_tag="VEP73_02290"
     CDS             4262..>4497
                     /locus_tag="VEP73_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25301.1"
                     /translation="MSQDRELPAQPQPPQTPPGELRSDPLTGRVVAIASGRAARPEAF
                     LRDVGPARGTLGCPFCPGNEHMTPPEVWADREPGG"
BASE COUNT          575 a         1591 c         1700 g          631 t
ORIGIN      
        1 cgacccgcag cccgcgccgg gcggcgatcg cccggacctc cggcttggtc agcgagccga
       61 gcggcaggcg caggtgtgcc aactgctccg gccccagcat gtagaggacg taggactggt
      121 ccttggccgg gtcggccgcg cggtggagcc gtggccgagg ctgaagcgga ccgtctcgcg
      181 ggcctcggtc ggggtgcggc ccatgaccag caacacatgg gaggcactga cggcgccgct
      241 ggcacaggcc gacccggccg aggcggcgat cccggcacgg tcgagcaaca gcagcaggtc
      301 ctcggagcgg accccgggga agcggaccga gagcaggccg ggcagcccgc cggtcgcgct
      361 gggatcgcca ttgacctgga ggccgggcgc ggcaccgcgg agccctcgca ggaggcggtc
      421 gcggagggcg gcgaggactg cgtcgccacc ccgctcccgc tcggccaggg ctgccgcggc
      481 ggcggcgccg aagcccacga tggccgccac cgggagagtg ccggagcgga ggccccgctc
      541 ctgcccgcca ccatgcagca gcgggcgcag cggggcgggg ttgctggtgc cgccgaggta
      601 ggggtcgctc cctccacctg ctctggaacg aatcaccagg gcgcccacgc cggtggggcc
      661 gccgagctta tgggcggcga gggcgagcag gtcgatgccg tcgcggtgca ggtcgacgcc
      721 tggcaggccg gctgcctgga ccgcgtcgca cagcagcaca gccccgacgg cgtgggccag
      781 cgtggcggcg agcgcgactg gctggacggt cccgacctcg ttgttggccg ccatgaccgc
      841 gaccagcgcg gtccggcccg gctccagcag ttcgctgagc tggtcgagga ggacgcgtcc
      901 cagcgggtcg accggtgcga agtggaggtg ggcgccggtc tcggcgtccg cccaacgtgc
      961 cgcgtcgagc acggcgtggt gctcaaccgt ggtgatgacc agttcccgct tgtcagggtc
     1021 acgggcgaat acggtgccct tgatggcgag attgtcggcc tcggtgcctc ccgaggtgaa
     1081 gaccacctcg gccgggtcgg cgccgaccag ggcggcgacc tgctcgcggg cggtctcgac
     1141 cgcgtgccgc gcggcccggc caggcccgtg ctggctggag gggttggcga ccggcgcctc
     1201 caggtacggc cgcatcgcct ccagcgcctc cgcgcggagc ggggcgctgg cggcatagtc
     1261 caggtacggc actagccaag tgtggcactg ggcgggtcgt cagtgctcgt cgcgcccctg
     1321 gcctcgctgg tctccctcgg ctcgccgggc tggctggggc tgggaggttc gtcccagcgc
     1381 tggtgctcga cctcctgcgg ttcggcgtgc cattcggcct gctcggcctg gcgggacggt
     1441 agccaccaga agttgtcctc ctcttcccag gttggctctg ccgctggcat ctgctgggtc
     1501 gagtcggagt ttcctgccgg gtcgatgtgg tcgccgccaa gcacgctcag gatgcggccg
     1561 gagcggccgc gaatccctgt tgtggaggcc gaggctccct ccacctctgt ctccgctgat
     1621 tggccggcgc cggggccagg gcgggaagcg cgtttgtcag cggcccgttc cacgcggatg
     1681 cgctcgacca tgcggtcgat tcgggcagcg gcggcgctgc cagccgccag cagggcggcg
     1741 ccgacccggg agccgacccg ggaaaggccg gtccggctgg cgcgggtcgc gtcggtgagg
     1801 ccggccgcga tgctgcgctt ggcggtctcc gccgccgggc cggcctggcg gcgggcgcgt
     1861 gccgtcgtcg ccagcagcca ggcccacacg cggtttgcga gcgcgacggt gacatcacgg
     1921 agcactgcgg cgaaatggcc ggcggcggcg agcgcctcgc gccagttgga ggcgagtgcc
     1981 gcgtgggcca gggtcaggtc atggtgcgct gtcgcggcgt ccgagcccgc cgaacgcacg
     2041 cgccagcggc gcgccggctc gccggcgagc aggcggacga cctgtccagc agcccgaagg
     2101 aacatctgtg ccgaaacgcc catcgatggg cgggtagtcg tgacgggctc gtggtggggg
     2161 ggcatccggg caacctccat ctctcggatc gcgccatcct acgatcccca gcgcgaaacc
     2221 gcccaaacac gcacagccat ttgactaccg gttggtcgcc cgtcagccgc ccttgcgttt
     2281 ggcgatctcg tcggtgaggc gcgggacgac tttgaaaagg tcgccgacca ggccgaagtc
     2341 ggcgatggcg aagatcgggg catcggcgtc cttgttgatc gccacgatcg tcttggaggt
     2401 ctgcatgccg gcgcggtgct ggatggcgcc ggagatcccg accgcgacgt agagctgcgg
     2461 cgagacggtc ttaccggtct ggccgacctg gtgctggtgg ggataccagc cggcgtccac
     2521 ggcggcgcgg ctggcgccga cggccgcgcc gagctggtcg gccagcgctt cgaccagtgc
     2581 gaagttcgcc gggtcctgca gcccccggcc gccggagacg atggtctcgg cctcggcgac
     2641 gtcggggcgg ccgccgcgct cctcgaccac gtggtcgatc acgcggatct cggcacctgt
     2701 cgcctcgcgc tggccgaggt cgacgacctg cggctcggcg ggcgcctcct gtgccgggaa
     2761 cgagttgggc ttcaccgcca gcaccgggac gccggtggtg acccgggcgc ggacgatggt
     2821 ggcgccgccg aagacgttct gggtcgcaac caggccgcct ccgtcaccgg atccggtagc
     2881 ggagtcgatg tcggagacgt cggccagcat gccccagccc ttgcgggcgg ccagccgggc
     2941 gccggcgtcc ttgcccgccg gggtggcggc gaccagcacc gcggccggct cggcgcggga
     3001 ggccgccgcg tcgagggcgt cggtcagcgg cccggggagc tggttgctcc agccctcgcc
     3061 ggtggcggcg tagatggtgg cggcgccgtg gcgggcgagg ccgtcacggg cgctgtcgag
     3121 cccctcgccg acgaacaccg ccgccggctc gcccagctcg cgtgccttgg ccagcatctc
     3181 ggcgctgacc ttcttgaggt tgccctcctc gtgctccacc aggacgagga tcgtggccat
     3241 gccgtgcctc ccggaactag gacgaagact tagatgaact tgtgcgcggc caggaactcg
     3301 accagctcgg tcgcggcggc gccgtcgccc gggtccttga ccacctggcc agactggcgc
     3361 ggcgggcgcg aggtggcgtc caccaccgcg gtggtggcgc cggcgctgcc gacctcgccg
     3421 gggtcgatgc cgaggtcggc gatggtcagg tcggtcaccg ggcgggactt ggccgccatg
     3481 atgccccgga acgacgggta gcgcggctcg ttgatcccct tgaccacgct gacgatggcc
     3541 gggccctggg cctccagcac gtcgtagccg gcctcggtct cgcggtggat gcggacggtg
     3601 gcaccctcga cctccagctg cttggcgccg atcaggcccg gccagccgag caactcggcg
     3661 accgcgctcg gcaccagcga gccgcgcgcg tcggtcgact ccgacccgga caacaccagg
     3721 tcggggccga cgtggcggat cgccgccgcc agcaccttgg cagtggccag cgcgtcagag
     3781 ccgtgcaggg cgtcgtcatg gacgtgcact gctgcgtccg ggcccagcga cagcgccttg
     3841 cggatagcag gcagggcgct ctcggggccc atgcacagca ccacgacctc gccctccccg
     3901 gaggattcct tgaggcggag cgcctcctcg acggcgtact cgtcgaccgg gttcatgatc
     3961 gtctcgaccg attcccggtc gagcgtgtag tcggccgggt ccagccgctt ctcggcagcg
     4021 gtgtccggca cccgcttgac ggtgaccagg atcttcacgg gttcgtccct ccatgctggt
     4081 tcgggcgccc atcatgccgc aatcgagggc gtcctcgcgg cgcgtggcgc ctacttgggt
     4141 gccaaactgg gaatagaaaa gaagggccgc gaactccccg attggctgtc tgtggcccct
     4201 cgctcatagg cttttgcgcg accggtccat tcccgagacg agaagcgaga cgaccatccc
     4261 cgtgagccaa gaccgagaac tcccagccca gccgcagccg ccgcagactc cgcctgggga
     4321 gctgcgctcc gaccccctga ccgggcgtgt ggtcgcgatc gccagcggcc gggccgcgcg
     4381 gcccgaggcg ttcctgcgcg acgtcggccc ggcgcgcggc acgctcggct gccccttctg
     4441 ccccgggaac gagcacatga ccccgccgga ggtctgggcc gaccgggagc cgggcgg
//
LOCUS       DASPYY010000152         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_307189, whole genome shotgun sequence.
ACCESSION   DASPYY010000152 DASPYY010000000
VERSION     DASPYY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307189"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VEP73_02295"
     CDS             <1..1195
                     /locus_tag="VEP73_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HYT25302.1"
                     /translation="PPYVPISLGALAGRVFEPVRYSPMQPWHEAHGARPLVAGQWIRP
                     DSYGDPAAEVRNVRANVGLIDVTPLGKLDLRGPDVPKLLNLLYVNKWSKLDVGSVRYG
                     VMCAEDGVVMDDGVTGHLDDDRYLMTTTSSGAATVWELVENWLQTEHPDWRVHVTPVT
                     TAYASINVAGPKSRELLGRVAEGVDLSPEAFPYMRVRLSTVAGVPDCFMWRIGFTGEL
                     SYEIHVPAAYGLHVWEALMERGADLGVAPFGVEAQRIMRLEKGHFIVAQDTDGLTKGF
                     SAGLDWAIKLDKDDFVGKPELVWSRTPNGDMRLVGLQPVDGRVVPPEASQIVEGRNTI
                     VGRVTSSRMSPTLGRSICLGFVAPHLTEPGTVVTVHLPDGRRVPATVQAGHAHFDPEG
                     VRLRG"
     gene            1188..>1725
                     /locus_tag="VEP73_02300"
     CDS             1188..>1725
                     /locus_tag="VEP73_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HYT25303.1"
                     /translation="MASAPPVARSPIAPAEPVVVAGGWEVSGRRSTAPLRLVDCTPLA
                     KVLVRATPGGPVARHLGVGHGRSRCDEHGTLVVGAGPDEWLLLAAPGAEAEVAARVRE
                     VAASTSPGDAPEVVSVVELFTHGRALVRLTGAEAPRLLAKVCAVDLADRTTPDGTAFR
                     SSVAKVATDVVRADLHGTR"
BASE COUNT          214 a          677 c          611 g          223 t
ORIGIN      
        1 cccgccctac gtgccgatca gcctcggcgc gctcgccggg cgcgtcttcg agccggtgcg
       61 gtactccccc atgcagccct ggcacgaggc gcacggcgcc cggccgctgg tcgccggcca
      121 gtggatccgg ccggacagct acggcgaccc ggccgccgag gtccgcaacg tccgcgcgaa
      181 cgtgggcctg atcgacgtca cgccgctcgg caagctcgac ctgcgcggcc cggacgtccc
      241 caagctgctc aacctcctct acgtcaacaa gtggtccaag ctcgacgtcg gctcggtccg
      301 ctacggggtc atgtgcgccg aggacggcgt cgtcatggac gacggggtca ccggccacct
      361 cgacgacgac cgctacctga tgaccaccac ctcgtccggt gccgccaccg tctgggagct
      421 ggtggagaac tggctccaga ccgagcaccc ggactggcgg gtccacgtca ccccggtgac
      481 caccgcgtac gcgagcatca acgtggccgg cccgaagtcg cgcgagctcc tcggccgggt
      541 cgccgagggg gtcgacctgt cgccggaggc gttcccctac atgcgggtgc ggctcagcac
      601 cgtggcgggc gtgcccgact gcttcatgtg gcgcatcggc ttcaccgggg agctgagcta
      661 cgagatccac gtgcccgccg cgtacggcct gcacgtgtgg gaggcgctga tggagcgcgg
      721 cgccgacctc ggcgtcgccc cgttcggcgt cgaggcgcag cggatcatgc ggctcgagaa
      781 gggccacttc atcgtcgccc aggacaccga cgggctcacc aaggggttca gcgccgggct
      841 cgactgggcc atcaagctcg acaaggacga cttcgtgggc aagcccgagc tggtctggtc
      901 gcgcacgccc aacggcgaca tgcggctggt cggcctgcag ccggtcgacg ggcgggtcgt
      961 gccgcccgag gccagccaga tcgtggaggg ccgcaacacg atcgtcgggc gggtgacctc
     1021 gagccggatg tcgcccaccc tggggcgctc gatctgcctc ggcttcgtcg ccccgcacct
     1081 gaccgagccg ggcaccgtcg tcaccgtcca cctgcccgac gggcgccgcg tcccggccac
     1141 cgtccaggcc ggccacgccc acttcgaccc cgagggggtg cgcctccgtg gctagcgcac
     1201 cgcccgtcgc ccgcagcccg atcgcccccg ccgagccggt cgtcgtggcc ggcgggtggg
     1261 aggtctccgg gcgccgctcg accgcgccgc tgcgcctggt cgactgcacg ccgctcgcca
     1321 aggtgctcgt ccgcgcgacc cctggcgggc cggtcgcccg ccacctcggc gtggggcacg
     1381 ggcggtcgag gtgcgacgag cacggcacgc tcgtcgtcgg cgccgggccc gacgagtggc
     1441 tgctcctcgc cgcgcccggc gcggaggccg aggtcgccgc gcgcgtccgc gaggtcgccg
     1501 cgtcgacctc gccgggcgac gccccggagg tcgtcagcgt cgtcgagctg ttcacccacg
     1561 gccgcgcgct cgtccgcctc accggcgccg aggccccgcg cctgctcgcc aaggtgtgcg
     1621 cggtcgacct cgccgaccgg acgacgcccg acggcaccgc cttccgcagc tcggtggcga
     1681 aggtcgcgac cgacgtcgtc cgcgccgacc tgcacggcac ccgct
//
LOCUS       DASPYY010000153         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2133612, whole genome shotgun sequence.
ACCESSION   DASPYY010000153 DASPYY010000000
VERSION     DASPYY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133612"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VEP73_02305"
     CDS             complement(<1..1162)
                     /locus_tag="VEP73_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HYT25304.1"
                     /translation="MVARVVVAGTHSGVGKTTVATGLMAAFAARGRAVAGFKVGPDFI
                     DPSYHALATGRPGRNLDAFLSGPELVAPLLAHGSSGADLAVVEGVMGLFDGAQGGGEL
                     ASTAQVARLLGAPVVLVVDAAGMARSVAAVVHGFATWDPGVRLAGVILNRVGSDRHEE
                     LLREALAPDGPPVLGVLRRRDALATPERHLGLVPAAERRAQAARTVAELGRVLAAAAD
                     LDAIAALAAAAPALPATAWDPATAAAVAGEARAGRARVAVAAGPAFTFVYEENLELLR
                     ACGVELAGFDPTADEALPEGAGGLYLGGGFPETYGEALAANRSLRAAVRAFATAGRPV
                     VAECGGLLYLGRSLDGRPMCGVVAAEGRMAGRLTLGYRRAVAAAPSVLAGAGR"
     gene            complement(1156..>1556)
                     /locus_tag="VEP73_02310"
     CDS             complement(1156..>1556)
                     /locus_tag="VEP73_02310"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYT25305.1"
                     /translation="SGKWKIGERKAAEALGGIDWESMGDGWTWVSRDLDQSADLAREG
                     WEEVKRRIADQTYDLLVLDEFTYPMAFGWVDAAEVVEVLRDRPGFQHVVVTGRDAPEA
                     LVEAADLVTEMRKVKHPFDEGVRGQQGIEW"
BASE COUNT          189 a          615 c          589 g          163 t
ORIGIN      
        1 gccgtcccgc gccggcgagc accgacgggg ccgcggcgac ggcccgccgg tagccgaggg
       61 tgagccggcc ggccatgcgc ccctccgccg cgacgacccc gcacatcggc ctgccgtcca
      121 gggagcggcc gaggtagagc agcccgccgc actcggcgac cacgggccgg ccggcggtcg
      181 cgaacgcgcg cacggcggct cggagggacc ggttcgcggc cagcgcctcg ccgtaggtct
      241 ccgggaagcc gccgccgagg tacaggccgc ccgccccctc gggcagggcc tcgtcggcgg
      301 tcgggtcgaa gccggccagc tccaccccgc aggcgcgcag cagctcgagg ttctcctcgt
      361 agacgaaggt gaaggccggc ccggcggcga ccgcgacacg ggcccgccct gcccgtgcct
      421 ccccggccac ggcggcggcc gtggccgggt cccacgcggt ggcgggcagc gcgggggcgg
      481 cggccgcgag ggcggcgatc gcgtcgaggt cggccgctgc cgcgagcacc cggccgagct
      541 cagcgacggt ccgggccgcc tgggcgcgcc gctcggcggc cgggacgagg ccgaggtgcc
      601 gctccggggt cgccagggcg tcgcggcggc gcagcacgcc gaggacgggt gggccgtccg
      661 gcgcgagcgc ctcgcggagc agctcctcgt gccggtcgga cccgacccgg ttgaggatca
      721 cccctgccag gcgcacgcca gggtcccagg tggcgaagcc gtggacgacg gccgcgaccg
      781 accgggccat ccccgcggcg tccacgacga gcaccacggg cgcgccgagg agcctcgcga
      841 cctgggcggt ggaggcgagc tccccgccgc cctgggcgcc gtcgaacagg cccatcacgc
      901 cctcgacgac ggccaggtcc gcgccgctcg agccgtgggc gagcagcggg gcgaccagct
      961 cgggcccgga gaggaaggcg tcgaggttgc gtccgggccg gccggtcgcg agcgcgtggt
     1021 aggacgggtc gatgaagtcc ggcccgacct tgaagccggc gaccgcgcgg ccccgcgccg
     1081 cgaacgccgc catcagcccg gtggccacgg tcgtcttgcc caccccggag tgggtgccgg
     1141 ccaccacgac ccgggctacc actcgatccc ctgctggcct cggacgccct cgtcgaacgg
     1201 gtgcttcacc ttgcgcatct cggtcaccag gtcggcggcc tcgaccagcg cctcgggggc
     1261 gtcccggccg gtgacgacga cgtgctggaa gccaggccgg tcgcggagca cctcgaccac
     1321 ctcggcggcg tccacccagc cgaacgccat cgggtaggtg aactcgtcca ggacgagcag
     1381 gtcgtaggtc tggtcggcga tccgccgctt gacctcctcc cagccctctc gggccaggtc
     1441 ggccgactgg tcgaggtccc gcgacaccca ggtccagccg tcgcccatgc tctcccagtc
     1501 gatgccgccg agcgcctcgg cggccttgcg ctcgccgatc ttccacttgc ccgact
//
LOCUS       DASPYY010000154         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2274914, whole genome shotgun sequence.
ACCESSION   DASPYY010000154 DASPYY010000000
VERSION     DASPYY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274914"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1683)
                     /gene="pgi"
                     /locus_tag="VEP73_02315"
     CDS             complement(52..1683)
                     /gene="pgi"
                     /locus_tag="VEP73_02315"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYT25306.1"
                     /translation="MAAAPSRITGTPEWRALEEHHAKLADLHLRTLFADDPGRGETMA
                     LEAVELYLDYSKHRATGETLRLLVALAERAGLRRRIDAMFAGEKINLTEGRPVLHVAL
                     RAPEGTSIVVDGVDVVPQVHAVLHKMGEFAVRVRSGEWTGHTGRRIANVVNIGIGGSD
                     LGPAMAYEALEDYSDRSLTVRFVSNVDGADVWEATRDLDPAETLFIVCSKTFTTLETL
                     TNARTARDWLLAALGDRDAVRRHFVAVSTNAEKVEEFGIDRANMFEFWDWVGGRYSMD
                     SAVGLSLMVAIGPEHFRAMLDGFHAMDEHFRTTPFDRNMPVLLGLLGVWYDDFFGAET
                     LAVLPYSQHLSRFPDYLQQLDMESNGKSVDLQGNPVTVQTGPVVWGQPGTNGQHAFYQ
                     LIHQGTKLVPCDFIGFCRPDHAVGGHHDLLMANFLAQTEALAFGKTSEEVAAEGVPPE
                     LVPHRTFPGNRPTSTILAPKLTPGVLGQLVALYEHKVFTQGTIWGVNSFDQWGVELGK
                     VLATRIVPQLEAPAEPGLDHDSSTNTLIRRYRRCR"
     gene            1905..>2372
                     /locus_tag="VEP73_02320"
     CDS             1905..>2372
                     /locus_tag="VEP73_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT25307.1"
                     /translation="MPPSPLITPEALAAELGAAPPVPRVLDARWQLGTDLGRAAYREG
                     HLPGAVFVDVDRDLSAPPGRGGRHPLPEPSVFEAAMRRRGVGDGRSVVVYDQGGAGGA
                     ARAWWLLTYFGHGAVRVLDGGFAAWAAAGLPVTAAVPRPAPGDFTARPGHRPVL"
BASE COUNT          300 a          855 c          885 g          332 t
ORIGIN      
        1 tcgggcgtcc ggcgccggcc gcggaacccc cgggagacga acccgcccat gtcagcggca
       61 cctccggtag cggcggatca gggtattggt cgagctgtcg tggtcgaggc cgggctctgc
      121 cggcgcctcg agctgcggca cgatccgggt cgcgaggacc ttcccgagct cgacgcccca
      181 ctggtcgaag gagttgaccc cccagatcgt gccctgggtg aagaccttgt gctcgtagag
      241 cgcgacgagc tgcccgagca cgccgggagt gagcttcggg gcgaggatcg tcgacgtcgg
      301 ccggttgcca gggaaggtgc ggtgggggac cagctcggga gggacgccct cggccgcgac
      361 ctcctcgctc gtcttgccga acgcgagcgc ctcggtctgg gcgaggaagt tggccatcag
      421 gaggtcgtgg tggccgccga cggcgtggtc cggccggcag aagccgatga agtcgcaggg
      481 gaccagcttc gtcccctggt ggatgagctg gtagaaggcg tgctgcccgt tcgtgcccgg
      541 ctggccccag accaccggcc cggtctgcac ggtgaccggg ttgccctgga ggtcgaccga
      601 cttgccgttg gactccatgt cgagctgctg caggtagtcg gggaagcggg acaggtgctg
      661 gctgtagggg aggacggcca gggtctcggc cccgaagaag tcgtcgtacc agacgccgag
      721 caggccgagg aggaccggca tgttgcggtc gaacggcgtg gtccggaagt gctcgtccat
      781 cgcgtggaag ccgtcgagca tggcgcggaa gtgctccggc ccgatcgcga ccatcagcga
      841 caggccgacc gccgagtcca tcgagtagcg cccgccgacc cagtcccaga actcgaacat
      901 gttcgcgcgg tcgatgccga actcctcgac cttctccgcg ttggtcgaca cggcgacgaa
      961 gtgcctgcgc actgcgtccc tgtcgccgag cgcggcgagc agccagtccc gggcggtgcg
     1021 ggcgttggtg agcgtctcca gggtcgtgaa cgtcttcgag cagacgatga acaacgtctc
     1081 ggccgggtcc aggtcccggg tcgcctccca gacgtcggcg ccgtcaacgt tggagacgaa
     1141 ccggacggtg agcgagcggt cgctgtagtc ctcgagcgcc tcgtacgcca tggccgggcc
     1201 gaggtcggag ccgccgatgc cgatgttgac gacgttggcg atccgcctgc cggtgtggcc
     1261 cgtccactcg cccgagcgga cccggacggc gaactcgccc atcttgtgga gcacggcgtg
     1321 gacctgcggg acgacgtcga ccccgtcgac gacgatcgac gtgccctcag gggcccgcag
     1381 cgcgacgtgc agcacggggc ggccctcggt gaggttgatc ttctcgccgg cgaacatggc
     1441 gtcgatgcgc cgccggaggc cggcccgctc ggccagcgcg acgagcagcc gcagcgtctc
     1501 cccggtggcg cggtgcttgg agtagtcgag gtacagctcg accgcctcga gggccatggt
     1561 ctcgccgcgg cccgggtcgt cggcgaacag cgtccggagg tggaggtccg cgagcttggc
     1621 gtggtgctcc tcgagcgccc gccactcggg cgtcccggtg atcctgctcg gtgccgctgc
     1681 catcgcccgt ctcctccgcc tgtctcctcc gacgccggtc cggctcatgc ccccggcggc
     1741 ctcgcgaccg tccccgcccg gggaccatac acgggcgcgc caggtacgac gatgcgccga
     1801 tcggcgcgag agggccaccc gccgtcgacg gccgccggct ggggcggtgc ccggccgcta
     1861 tcctcggcag gcgacctccg tccgagcgac caaggagccg accggtgccc ccgagccccc
     1921 tgatcacccc cgaggcgctc gccgccgagc tgggcgccgc cccgccggtc ccccgcgtgc
     1981 tcgacgcgcg ctggcagctc ggcaccgacc tcggccgcgc cgcctaccgc gagggccacc
     2041 tgcccggcgc ggtattcgtg gacgtcgacc gggacctgtc ggcgccgccg gggcgcggcg
     2101 gccgccaccc gctgcccgag ccgtccgtgt tcgaggcggc catgcgccgc cgcggcgtcg
     2161 gcgacggccg ctccgtcgtc gtgtacgacc agggcggtgc cgggggcgcg gcacgcgcgt
     2221 ggtggctcct cacctacttc gggcacggcg cggtcagggt gctcgacggc gggttcgccg
     2281 cctgggcggc ggccgggctg ccggtgaccg ccgcggtgcc acggcccgcg ccgggcgact
     2341 tcacggcccg ccccggccac cggcccgtgc tc
//
LOCUS       DASPYY010000155         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_307515, whole genome shotgun sequence.
ACCESSION   DASPYY010000155 DASPYY010000000
VERSION     DASPYY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307515"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VEP73_02325"
     CDS             <1..559
                     /locus_tag="VEP73_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25308.1"
                     /translation="VEDLLALARLDGGTARRREAVDLSGVVREVAGRVAAPRVALRVA
                     AAPGIEVRGDAEGLARAVQNLLDNALRHAATRVEVSLRTEGALAVLAVADDGPGVPAA
                     DRERVFERFARLDDARSRDAGGTGLGLAIVREVVRAHGGEAALEDGAPGARFVAWLPA
                     MRTGAPPGPGSPAGPVTAARRAAGA"
     gene            complement(528..1046)
                     /locus_tag="VEP73_02330"
     CDS             complement(528..1046)
                     /locus_tag="VEP73_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYT25309.1"
                     /translation="MPTSRSLVAATLARAAAIGAAGVVVHAGYEMGGGRASGKGSMAA
                     RFEEAAEQLAACDDHPRLAVCLDTCHAHAAGYDLTTPAAATAVLDELVRTVGDRVPLL
                     HVNDTRDPLGARRDRHWPVGEGEIGDAGFAAVLAHPGLAGAAAVIETPGELPEHARDV
                     ARLKRLRPAAPR"
     gene            complement(1059..>2268)
                     /locus_tag="VEP73_02335"
     CDS             complement(1059..>2268)
                     /locus_tag="VEP73_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYT25310.1"
                     /translation="PPPSLSVQVPQPVDAAIWRATDPDPARRHARASDFAAAIGAPRR
                     RRVDEALRAATVVPPAAWQAGSETTQVEGPAYQPAGGPAPMAPAPPGPLRPRPTTGRV
                     PRGRPERERGSRWRRRLGTALVALAVVAAAIGGTLAFVASRTVEMPDVVGQPVTVARV
                     RLQANGLRVSVGAPIPSPSVPKGRVARQSVFPAVTVHRLSRVRLFPSAGILMPDLRNQ
                     GAGSARATLDRLGVAYAATVQASRSVPAGEVIGSDPPAGRLLDRQQVRLVVSSGPPRV
                     AVPDVLGATFADAKRALVAARLRVHRTRVFSGDVPPGRVVQTSPQAGSSAVEGTVVDV
                     QTSKGPDLVVVPEVRGRPRDEAQRLLQALGFQVSFSFPGFGSRVTDQSLQPGTQVPRG
                     TAITLSLSLF"
BASE COUNT          239 a          877 c          927 g          225 t
ORIGIN      
        1 ggtcgaggac ctgctcgcgc tcgcccggct cgacggcggg acggccaggc ggcgggaggc
       61 ggtggacctc tccggcgtcg tccgggaggt cgccgggcgc gtggccgcgc cccgcgtcgc
      121 cctccgggtc gccgccgcgc caggcatcga ggtgcggggc gacgccgagg ggctcgcccg
      181 cgccgtgcag aacctgctcg acaacgcgct gcgccacgcg gccacccgcg tcgaggtctc
      241 gctgcggacc gagggcgccc tcgccgtgct ggcggtcgcc gacgacgggc ccggcgtgcc
      301 cgcggccgac cgcgagcggg tgttcgagcg gttcgcgcgc ctcgacgacg cgcgcagccg
      361 ggacgcgggc ggcaccggcc tcgggctcgc catcgtgcgg gaggtcgtgc gggcccacgg
      421 cggcgaggcg gcgctcgagg acggcgcccc cggcgcgcgg ttcgtcgcct ggctcccggc
      481 catgcggacc ggcgcgcccc ccggccccgg gtccccggcc ggcccggtca ccgcggcgcg
      541 gcgggccgca ggcgcttgag gcgcgccacg tcgcgggcgt gctcgggcag ctcgcccggg
      601 gtctcgatca ccgcggcggc cccggccagg ccggggtggg cgagcaccgc ggcgaagccg
      661 gcgtcgccga tctcgccctc accgacgggc cagtgccggt cgcgcctggc gcccaggggg
      721 tcgcgcgtgt cgttgacgtg gagcagcggg acccggtcgc cgaccgtgcg gacgagctcg
      781 tcgaggaccg cggtggccgc cgccggggtg gtgaggtcgt acccggccgc gtgggcgtgg
      841 caggtgtcga ggcacacggc caggcgcgga tggtcgtcgc aggcggccag ctgctccgcg
      901 gcctcctcga accgggcggc catcgacccc ttgccgctcg cacgtccgcc gcccatctcg
      961 tacccggcgt ggaccaccac cccggccgcc ccgatggcgg ccgcgcgcgc cagggtcgcg
     1021 gcaaccagcg accgggacgt gggcaccgac acgcacggtc agaacagcga gagcgacagg
     1081 gtgatcgccg tgccccgggg cacctgggtg cccggctgca ggctctggtc ggtcacgcgg
     1141 ctgccgaagc ccgggaacga gaagctcacc tggaacccca gcgcctgcag gaggcgctgg
     1201 gcctcgtccc tgggccgccc gcggacctcg ggcacgacga cgaggtcggg gcccttgctc
     1261 gtctggacgt cgacgaccgt gccctcgacg gcgctcgagc ccgcctgcgg gctggtctgc
     1321 acgacgcggc cgggcggcac gtcgccggag aacacccgtg tccggtgcac gcgcaggcgg
     1381 gcggccacga gcgcgcgctt cgcgtccgcg aaggtggcgc ccagcacgtc gggcacggcg
     1441 acgcgcgggg ggccgctgga cacgacgagc cgcacctgct ggcggtcgag cagccggccg
     1501 gccggagggt cggagccgat gacctcgccg gcgggcacgc tccggctcgc ctggacggtc
     1561 gcggcgtagg ccacgccgag ccggtcgagg gtcgccctgg cgctgccggc accctggttg
     1621 cgcaggtcgg gcatgaggat cccggccgag gggaagagcc ggaccctgga caggcggtgc
     1681 acggtcaccg ccgggaacac cgactggcgc gccaccctgc ccttgggcac gctcggggac
     1741 gggatcggcg cgccgacgct gacccgcagg ccgttcgcct ggagccggac cctggcgacg
     1801 gtgaccggct ggccgacgac gtcgggcatc tcgaccgtgc ggctcgcgac gaaggcgagc
     1861 gtgccgccga tcgcggcggc gacgaccgcg agcgccacga gcgcggtgcc gagccggcgg
     1921 cgccagcggg agccgcgctc ccgctccggg cgacccctcg gcacccgccc ggtggtcggg
     1981 cgggggcgca gcggcccggg cggagcgggc gccatcgggg ccggcccgcc agccggctgg
     2041 tacgcggggc cctccacctg cgtcgtctcc gacccggcct gccaggccgc cggggggacc
     2101 accgtggcgg cgcgcagcgc ctcgtccacg cgccgccgcc tgggcgcccc gatcgccgcg
     2161 gcgaagtcgg acgcgcgcgc gtggcggcgg gccgggtcgg ggtcggtggc ccgccagatc
     2221 gcggcgtcca ccggctgcgg cacctgcacg ctgagcgacg gcggcggc
//
LOCUS       DASPYY010000156         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1291798, whole genome shotgun sequence.
ACCESSION   DASPYY010000156 DASPYY010000000
VERSION     DASPYY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291798"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VEP73_02340"
     CDS             complement(<1..265)
                     /locus_tag="VEP73_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25311.1"
                     /translation="MEDGRISWIRPSDDAGPKDSADVPDGTLEVVDASGSVFVPGLVD
                     CHSHLTLPGGAHWIDRIDDPPERLLAVAEHNARLLRQAGVRWAR"
     gene            complement(353..1372)
                     /gene="gnd"
                     /locus_tag="VEP73_02345"
     CDS             complement(353..1372)
                     /gene="gnd"
                     /locus_tag="VEP73_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYT25312.1"
                     /translation="MQLGMVGLGRMGANIVRRLMRGGHECVVWTRNQATVAQLAGEGA
                     TGSADLDEFVAKLATPRAVWIMVPAAATDDMIGQLAARMSAGDVIIDGGNSYYHDDIR
                     RSKELAGKGIHYVDIGTSGGVFGLERGFCLMIGGEDDVVGRLDPLFRTIAPGVEAAPR
                     TPGRTGEPSPAERGYLHCGPHGAGHFVKMVHNGIEYGIMAAYAEGMNILSHANVGNAD
                     RAVSAETTPLCHPELYRYDLDIPAVAEVWRRGSVVASWLLDLTAHALSVSPDLAAFGG
                     RVSDSGEGRWTLLAAIDEGVPTHVLSAALYDRFESRGEAEFANRLLSAMRKEFGGHEE
                     LPTGS"
     gene            1595..>1847
                     /locus_tag="VEP73_02350"
     CDS             1595..>1847
                     /locus_tag="VEP73_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25313.1"
                     /translation="MADGPVTWGRPVPSGRPGVYVVEWPQARADAPIELTLVGKWLER
                     LPGLRLDGHRPTTRALAARLHDFWLPGQAVVYVGMTTGSL"
BASE COUNT          253 a          679 c          651 g          264 t
ORIGIN      
        1 cgcgcgccca gcggacgccg gcctgccgga ggagccgtgc gttgtgctcg gcgacggcca
       61 ggaggcgctc gggcggatcg tcgatgcgat cgatccagtg ggcgcctcca ggcagggtca
      121 ggtggctgtg gcagtcgacc aaccccggaa cgaacaccga accgctcgcg tcgacgacct
      181 cgagcgtgcc gtcgggcacg tcggcggaat ctttcgggcc ggcgtcgtcg gacggtcgaa
      241 tccacgagat ccggccatcc tccacgagga tgctcacgcc gagctccagc cggtcggacc
      301 ggccgtccgc cagggcgccg tcccggtaga gcgtcctcac gccgggcccg gcctaggagc
      361 cggtgggcag ctcttcgtgc ccgccgaact ccttgcgcat ggcggacaga aggcggttcg
      421 cgaactcggc ctcgcctcgc gactcgaagc ggtcgtacag ggcggccgac aggacgtgcg
      481 tggggacacc ctcgtcgatc gccgccagca gcgtccagcg gccctcgccg gagtcggaga
      541 cgcgaccgcc gaaggcggcc aggtcgggcg agacggacag ggcgtgcgcg gtcaggtcga
      601 gcagccacga agcaaccacg ctgccgcgcc gccacacctc ggcgaccgcc gggatgtcga
      661 gatcgtagcg atagagctcc gggtggcaga gtggcgtcgt ttcggcgctc acggcacggt
      721 cggcgttgcc cacgttcgca tgggacagga tgttcatgcc ctcggcgtag gcggccatga
      781 tcccgtactc gatcccgttg tggaccatct tgacgaagtg gccggcgccg tgcgggccgc
      841 agtgcagata gccccgctcg gccggcgaag gctcgccggt gcgcccgggc gtcctggggg
      901 cggcctccac ccccggcgcg atcgtccgga agagcggatc caggcggccg acgacgtcat
      961 cctcgccacc gatcatcagg cagaagcccc gctcgaggcc gaacacgccg ccactcgtcc
     1021 cgatgtcgac gtagtggatg cccttgccgg ccagctcctt ggaccggcgg atgtcgtcgt
     1081 ggtagtagct gttgccaccg tcgatgatga cgtcgccggc gctcatccgg gccgccagct
     1141 gtccgatcat gtcgtccgtg gccgccgccg ggaccatgat ccacacggcc cgcggcgtcg
     1201 ccagcttggc cacgaattca tccaggtccg cggaccccgt cgcgccctca ccggcgagct
     1261 gggcgaccgt cgcctggttg cgcgtccaga ccacgcactc gtggccgccg cgcatgaggc
     1321 ggcggacgat attcgcgccc atccgcccga gtccgaccat cccgagctgc atcggttctc
     1381 ctccgctgtc acagccgccg tggcgccggc gttcacccgt cgttcgtcag gggcaaggat
     1441 acggcgcctc acccgccacg ctgcgtccgg ggtagcattc ggcggcaccc cagctcgccg
     1501 ccaccgctcg aaggttgccg tgacttctca accgcccgcg ccgccccatg ctcggcccgt
     1561 cggcggagcg gaactgctgc gcgcggtggg cctcatggcg gacggcccgg tcacctgggg
     1621 ccggccggtg ccgagcggca ggcccggcgt ctatgtcgtc gagtggccgc aggcccgcgc
     1681 ggacgccccg atcgagttga cgctcgtcgg caagtggctc gagcggctgc ccggcctgcg
     1741 cctggacggc caccggccga cgacgcgggc actggccgcc cgcctccatg atttctggct
     1801 gcccggccag gcggtcgtct acgtcggcat gacgacgggt agcctgg
//
LOCUS       DASPYY010000157         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2134513, whole genome shotgun sequence.
ACCESSION   DASPYY010000157 DASPYY010000000
VERSION     DASPYY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134513"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VEP73_02355"
     CDS             <1..1893
                     /locus_tag="VEP73_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT25314.1"
                     /translation="DAERACEPGAPLVHDEGNVLVSWTIARGDVEAGLAAADLVVEGE
                     YRTQRVEHAYLEPEAGVGWVDTSGVVTLRVSTQVIEHAATIATILGLPESRVRVIGAY
                     MGGGFGGKEDMTVEPYLALLVWHTRRPVRLVFERQESLLATTKRHPFVMRYRTGVRRD
                     GTIVAQDVDIVGDAGAYPYLSARVLFAACVCASGPYRVPTVRHRTRAVFTNTPPTSAF
                     RGFGAMQVTFGYEQQLDRVAAALGLDPVAVRERNFLAKGDPLPTGERLGTAVAVGECL
                     QRALDALGEPARPSGPGRRVGRGVAANLQPYGRTVWFNDRAACWLSLEADGSLQLRSG
                     VTDLGGGQAASLAQIVSEILGVPLDEIGVHIGDTALTPPAGGTFATRQLYMSGNAALA
                     AARELRARLLPVAAALLGAGEDDLIFENGMVRQQGDGGGRSLHLAELVAACHQRGVPT
                     SHLGTWYAPTGSFDPVSGQGASFPDYTFGAHAAEVEVDLETGETRVLNYAAAHDVGRA
                     INPLRVEGQIQGGAYQGIGYALGEEIVLADGNATSTLLANYLMPNAVDLPDVRVVIIE
                     SGEGKGPFGARGIGEPPIANAAAALANAVAAATGARVTQLPITPERLLDALDALGMDT
                     GGGLTR"
     gene            1890..>2108
                     /locus_tag="VEP73_02360"
     CDS             1890..>2108
                     /locus_tag="VEP73_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HYT25315.1"
                     /translation="MTHDRLTRELVLNPVSALREVPPGPGLLPPPSRAPLALHRRLPG
                     YAVTPLREVPALAGACGVGRVWVRDESTR"
BASE COUNT          279 a          799 c          754 g          276 t
ORIGIN      
        1 gacgccgagc gggcgtgcga gccgggcgcg ccgctggtgc acgacgaggg gaacgtcctg
       61 gtgagctgga ccatcgcccg gggtgacgtg gaggcggggc tggcggcggc cgatctcgtg
      121 gtcgagggcg agtaccgcac ccagcgggtg gagcacgcct acctcgagcc cgaggcgggc
      181 gtcggctggg tcgacacctc cggggtcgtc acgctgcgcg tctccacgca ggtgatcgag
      241 cacgccgcca ccatcgccac catcctgggc ctgcccgaga gcagggtccg ggtgatcggc
      301 gcgtacatgg gcggcgggtt cggcggcaag gaggacatga ccgtcgagcc ctacctcgcg
      361 ctgctggtgt ggcacacccg ccggccggtc aggctggtct tcgagcgcca ggagtcgctg
      421 ctggccacca ccaagcgcca cccatttgtg atgcgatacc gcaccggcgt ccggcgcgac
      481 ggcaccatcg tcgcccagga cgtcgacatc gtcggcgacg ccggcgccta cccgtacctc
      541 agcgctcggg tgctgttcgc cgcctgcgtc tgcgcctccg gtccttaccg cgtgccaacg
      601 gtccgccacc ggacccgcgc ggtgttcacc aacaccccgc cgaccagcgc tttccgcggc
      661 ttcggcgcga tgcaggtgac gttcggctac gagcagcagc tggaccgggt cgcggcggcg
      721 ctcggcctgg acccggtcgc ggtgcgggag cgcaacttcc tcgcgaaagg cgatccgctc
      781 ccgaccggtg agcggctcgg caccgcggtg gcggtggggg agtgcctgca gcgggcgctt
      841 gacgccctcg gcgagccggc gcggccgagc gggccgggcc ggcgggtcgg ccgcggggtc
      901 gccgcaaacc tgcagcccta cggccgcacc gtctggttca acgaccgtgc ggcctgctgg
      961 ctcagcctgg aggccgacgg cagcctgcag ctccgcagcg gcgtcaccga cctcggcggc
     1021 ggtcaggccg cctccctggc gcagatcgta tctgagatcc tcggcgtgcc actcgacgag
     1081 atcggcgtgc atatcggcga caccgcactg accccgccag ctggcgggac cttcgccacc
     1141 cgccagctct atatgtcggg caacgccgca ctggccgccg cccgcgagct gcgtgcccgc
     1201 ctgctgccgg tcgccgccgc gctgctcggc gccggcgagg acgatctgat cttcgagaac
     1261 ggcatggtca gacagcaggg tgacggcggc gggcggagcc ttcacctggc tgagctggtc
     1321 gccgcctgcc accagcgcgg ggtgccgacc tcgcacctcg gcacctggta cgccccgacc
     1381 ggcagcttcg atccggtcag cggccagggc gccagcttcc ccgactacac cttcggcgcc
     1441 cacgccgccg aggtcgaggt cgacctggag accggcgaga cccgcgtcct gaactacgcc
     1501 gccgcgcacg acgtcggccg ggcgatcaac cccctccgcg tcgaaggcca gatccagggc
     1561 ggcgcctacc agggcatcgg ctacgcgctc ggtgaggaga tcgtcctggc cgacggcaac
     1621 gccacctcca cgctactggc caactacctc atgccgaacg cggtcgacct gcccgacgtg
     1681 cgcgtggtca tcatcgaaag cggcgagggc aaaggcccgt tcggcgcccg cgggatcggc
     1741 gagccgccca tcgccaacgc cgccgccgcg ctcgccaacg ccgtcgccgc agccaccggc
     1801 gcccgcgtca cccagctccc cattaccccg gaacggctcc tcgacgccct cgacgcgctt
     1861 ggtatggata caggtggagg gctgacgcgg tgacccacga ccggttgacc cgcgagctgg
     1921 tcctcaaccc ggtgtcggcg ctgcgcgagg tgccgccggg gccggggctg ctgccgccgc
     1981 cgtcgcgggc gccactcgca ctgcaccggc ggctgcccgg ctacgcggtc acgccgctgc
     2041 gggaggtgcc cgcgctcgcg ggggcatgcg gcgtcggccg ggtctgggtc agggacgagt
     2101 ccacccgc
//
LOCUS       DASPYY010000158         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_590092, whole genome shotgun sequence.
ACCESSION   DASPYY010000158 DASPYY010000000
VERSION     DASPYY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590092"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VEP73_02365"
     CDS             <1..1527
                     /locus_tag="VEP73_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYT25316.1"
                     /translation="VVGGRPVASTITWLTGLDGVPGPGRGRLAGRRVLASTGWLSVTP
                     GQEARLLVTTFSGPTAGGADSRLAALVLLLLVVAGLVATLLGAVLARLVSHPLNELAD
                     QARAIARGDFASPPSAVRSGGEVGELARAFDSMRVELGEYLDALRASRDELARSMTRL
                     GQTLSSTHDLPKLLEVVLEAAVRARRARAGSVLLLSADRSTLVRQATYGLAERELATR
                     IPVGHGIAGSVVATGKPVVVSVPGPAPAQAPAAARDARAVEPPAATQVSVPLLSQGRV
                     LGVLSVYDRETAEPFALPDASALADFAVQAAVAIENVQLHEEAERLSLTDAMTGTWNY
                     RYFERRFEQELERSRRFDRLFALLMLDVDHFKLVNDRHGHQRGDEVLMELARRIVSSV
                     RDIDTFARYGGEEFVLILPETNLAGGIATAEKLRDLANARPFTDRPGEGGVRLTLSIG
                     VACYPEHARSTSDLLRAADDAMYEAKMRGRDRVVVAGPARAPAPARLRPPRGRPARVE
                     "
     gene            1656..>2333
                     /locus_tag="VEP73_02370"
     CDS             1656..>2333
                     /locus_tag="VEP73_02370"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HYT25317.1"
                     /translation="MNVRKAVIPAAGLGTRFLPASKAQPKEMLPVVDKPAIQYVLEEA
                     VRAGLEDILIITGRGKGAIEDHFDRAWELEGVLAEKGDDEKLAEVRAISEMAEVHYIR
                     QKMPLGLGHAIGVAEQHVGAEPFAVLLGDDLIGEDEKLLTDLVAVFERHGTSVIAAME
                     VERSQIKSYGAITPGEEVEPGVIRVADIVEKPDPDEAPSNLATIGRYVLHPEIFDALR
                     DTRPDRKG"
BASE COUNT          316 a          877 c          843 g          297 t
ORIGIN      
        1 gtggtcggag gcaggccggt ggcgagcacc atcacctggc tgaccggcct cgacggcgtg
       61 cccgggccgg gcagggggcg gctcgccggc cgccgggtgc tcgcgtccac cggctggctg
      121 tcggtgaccc ccggccagga ggcgaggctg ctcgtcacca cgttctccgg ccccaccgcg
      181 ggcggtgccg acagccggct cgcggcgctg gtgctgctcc tgctcgtcgt cgcgggcctg
      241 gtcgcgaccc tgctcggcgc cgtcctcgcg cggctcgtgt cccatcccct caacgagctc
      301 gccgaccagg cccgcgccat cgccaggggc gacttcgcga gccctccgtc cgcggtgcgc
      361 tccggcggtg aggtggggga gctcgcccga gccttcgaca gcatgcgggt cgagctgggg
      421 gagtacctcg acgccctgcg ggcgagccgc gacgagctgg cccgctcgat gacgcgcctc
      481 ggccagacgc tctcgtcaac gcacgacctg cccaagctgc tcgaggtggt gctcgaggcc
      541 gccgtgcggg cgcgccgtgc ccgggccggg agcgtgctcc tgctctcggc cgaccggtcg
      601 accctggtgc gccaggcgac ctacgggctc gccgagcgcg agctggccac ccgcatcccg
      661 gtcggccacg gcatcgccgg gtcggtggtc gccaccggca agccggtcgt ggtctccgtc
      721 cccgggccgg cgccagccca ggcaccggcc gcggccaggg acgcccgggc ggtcgagccg
      781 cctgccgcca cccaggtgag cgtcccgctc ctctcccagg gccgcgtgct cggcgtgctg
      841 agcgtctacg accgggagac ggccgagccg ttcgcgctcc cggacgcctc cgccctggcc
      901 gacttcgccg tccaggccgc cgtcgccatc gagaacgtcc agctccacga ggaggccgag
      961 cgcctgtcgc tgaccgacgc gatgaccggg acctggaact accggtactt cgagcgccgc
     1021 ttcgagcagg agctcgagcg ctcgcgccgc ttcgaccgcc tcttcgccct cctgatgctg
     1081 gacgtcgacc acttcaagct ggtcaacgac cggcacggcc accagcgcgg cgacgaggtg
     1141 ctgatggagc tggcccgccg gatcgtctcg agcgtgcgcg acatcgacac cttcgcgcgc
     1201 tacggcggcg aggagttcgt gctgatcctg ccggagacca acctggccgg cgggatcgcg
     1261 acggccgaga agctccgcga cctcgccaac gcccggccgt tcaccgaccg ccccggggag
     1321 ggcggcgtgc gcctgaccct ctcgatcggg gtcgcctgct acccggagca cgccaggtcc
     1381 accagcgacc tgctgcgcgc ggccgacgac gccatgtacg aggccaagat gcgcggccgc
     1441 gaccgggtgg tcgtcgccgg cccggcccgc gcgcccgcgc cggcccgcct gcgcccgcca
     1501 cgcggccgcc cggcccgggt cgagtgagcc ggccgagcgt cgcagaagaa gcagcaccgg
     1561 cccgtattca gtagcatcgc ctcctcatgg aggcgcagcc tcgcggggtc gtcagggaga
     1621 gcgtgctcgg gggtcgacgt ggtcggggag tcgaagtgaa cgtacgcaag gccgtcattc
     1681 ccgcggcagg gctcgggacc cgattcctgc ccgcgtcgaa ggcgcagccc aaggagatgc
     1741 tgccggtcgt cgacaagccg gcgatccagt acgtcctcga ggaggccgtg cgggccgggc
     1801 tcgaggacat cctcatcatc accggccggg gcaagggggc gatcgaggac cacttcgacc
     1861 gcgcctggga gctcgagggc gtcctcgccg agaagggcga cgacgagaag ctcgccgagg
     1921 tccgggccat ctccgagatg gccgaggtcc actacatcag gcagaagatg ccgctcgggc
     1981 tcgggcacgc gatcggcgtg gccgagcagc acgtcggcgc cgagccgttc gccgtgctgc
     2041 tcggggacga cctgatcggc gaggacgaga agctgctgac cgacctcgtc gccgtcttcg
     2101 agcgccacgg gaccagcgtg atcgcggcca tggaggtcga gcggtcgcag atcaagtcct
     2161 acggcgcgat cacccccggc gaggaggtgg agcccggcgt gatcagggtc gccgacatcg
     2221 tcgagaagcc cgaccccgac gaggccccgt ccaacctggc caccatcggc cgctacgtgc
     2281 tccacccgga gatcttcgac gcgctgcgcg acaccaggcc cgaccggaag ggc
//
LOCUS       DASPYY010000159         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1713546, whole genome shotgun sequence.
ACCESSION   DASPYY010000159 DASPYY010000000
VERSION     DASPYY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713546"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VEP73_02375"
     CDS             complement(<1..745)
                     /locus_tag="VEP73_02375"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HYT25318.1"
                     /translation="MPRTTRDDLREHLVATRIAGSVQTPVWDVLRKAALVAAGDPDHC
                     FGLSGMSRNTQAEVLAQVTEQFGWTHTPGEPGDGRTFIDPDLVLDELDRAAERLARAA
                     RDGQHVVFATGHPTGPLGMYQQVADALRDAGAKVSRPGDGVAFLLGGRRRMVRYVRNV
                     AMLASGANLYHTHDAHPMQLVLDQAAAVDLVVGDHGFAGAAAERGIDVVSVADINDPA
                     LPMAKAEGRAGVVIGMDDNVLPHRYDPLAD"
     gene            complement(824..>1766)
                     /locus_tag="VEP73_02380"
     CDS             complement(824..>1766)
                     /locus_tag="VEP73_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HYT25319.1"
                     /translation="APRYGIGPGDTPSFAGMHEASALVCGATVAAAMAVVGGAQLDMP
                     TAGPRHAFSPAGGLHHAMRDRASGFCVYNDPAVAIAWMLEHGVGRVAYVDVDVHHGDG
                     VEAIFADEPRVLTVSVHESGRYLFPGTGFAQEVGDGPARGSIANLPLPPYTTDDLYLA
                     AFDAVVPALVRAFEPEVLVTQLGCDTHYTDPLAHLGLTVGAYRQLAGRLHDLAHTVAG
                     GRWLATGGGGYQWAAVVPRAWCGYLAEMVGSELPERLPDAYLAEAAERFGAELPPEMA
                     DDVVALAGEHRGRVERQVADAVETARRNLFPLHGLTV"
BASE COUNT          218 a          632 c          692 g          224 t
ORIGIN      
        1 ggtcggccag ggggtcgtac cggtggggga ggacgttgtc gtccatcccg atcaccacgc
       61 cggcgcggcc ctccgccttg gccatcggca gcgccgggtc gttgatgtcg gccaccgaca
      121 cgacgtcgat cccgcgctcg gccgccgcac cggcgaagcc gtggtcgccg acgaccaggt
      181 cgaccgccgc ggcctggtcg agcaccagct gcatcgggtg ggcgtcgtgg gtgtggtaga
      241 ggttggcccc cgaggcgagc atcgccacgt tgcggacgta cctcaccatg cggcggcggc
      301 cgccgagcag gaaggcgacg ccgtcgccgg gccgggacac cttcgcgccc gcgtcgcgca
      361 gggcgtcggc gacctgctgg tacatgccga gcgggccggt cgggtggccg gtggcgaaca
      421 ccacgtgctg gccgtccctg gccgccctgg cgagccgctc cgcggcccgg tcgagctcgt
      481 cgaggaccag gtcgggatcg atgaaggtgc ggccgtcgcc cggctcgccg ggagtgtggg
      541 tccagccgaa ctgctcggtg acctgggcga gcacctcggc ctgggtgttg cggctcatgc
      601 cggagaggcc gaagcagtgg tcggggtcgc cggcggcgac cagggcggcc ttgcgcagga
      661 cgtcccagac cggggtctgc accgacccgg cgatccgggt ggccaccagg tgctcgcgta
      721 ggtcgtcacg ggtcgtccgg ggcatgccag ctccatcgtc agcttcctgg ggcagcgctc
      781 atggtagcgg aggcggccgc cccggccctt ccgcgtcctg gcgtcagacg gtcaggccgt
      841 ggagggggaa gaggttcctg cgggccgtct cgaccgcgtc ggcgacctgc cgctcgaccc
      901 ggccccggtg ctcgccggcg agggcgacca cgtcgtcggc catctctggt ggcagctccg
      961 cgccgaaccg ctccgccgcc tcggcgaggt aggcgtcggg gagccgctcg ggaagctcgc
     1021 tgcccaccat ctctgccagg tagccgcacc aggcccgcgg cacgaccgcg gcccactggt
     1081 agcctccgcc gccggtggcg agccagcggc ccccggcgac cgtgtgggcg aggtcgtgga
     1141 ggcgcccggc gagctgccgg taggccccca cggtgagccc gaggtgggcg agcgggtcgg
     1201 tgtaatgggt gtcgcagccg agctgggtga cgagcacctc gggctcgaac gcgcgcacca
     1261 gcgcgggcac gaccgcgtcg aacgcggcca ggtagaggtc gtcggtcgtg tacggaggca
     1321 gcgggaggtt cgcgatcgac ccgcgggccg gtccgtcccc gacctcctgc gcgaagccgg
     1381 tgcccgggaa caggtaccgc ccggactcgt gcaccgagac ggtgaggacc ctcggctcgt
     1441 cggcgaagat ggcctcgacg ccgtcgccgt ggtggacgtc gacgtcgacg taggcgacgc
     1501 ggcccacccc gtgctcgagc atccaggcga tggcgacggc ggggtcgttg tagacgcaga
     1561 agccggacgc ccggtcgcgc atcgcgtggt gcaggccgcc cgcgggcgag aacgcgtgcc
     1621 gcgggcccgc ggtcggcatg tcgagctgcg cgccgccgac caccgccatg gccgcggcca
     1681 ccgtggcccc gcacaccagg gcggacgcct cgtgcatgcc ggcgaacgac ggcgtgtcgc
     1741 ccgggccgat gccgtaccgc ggcgcg
//
LOCUS       DASPYY010000160         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1573882, whole genome shotgun sequence.
ACCESSION   DASPYY010000160 DASPYY010000000
VERSION     DASPYY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573882"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2971
                     /locus_tag="VEP73_02385"
     CDS             <1..2971
                     /locus_tag="VEP73_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT25320.1"
                     /translation="SSWRIEEITRDRVVVTPAPGQPGKMPFWHGDGLGRPYELGRAIG
                     AFLGEVDGWSDERLAEECSLDDRAVRNLRAYLAEEREVTGAVPTDRQIVIERFRDELG
                     DWRVCVHSPFGAPLHAPWAQAIEARVRERLGLEVQTMYSDDGIVVRLPEADEAPPSDS
                     VLFEPEEVDDLVVGQVSGSALFASRFRECAARALLLPRRRPGRRTPLWQQRQRSTGLL
                     QVAAGYGSFPIVLETMRECLQDVFDLPGLAELMAAVGRREVRVVEVDTPFPSPFASSL
                     QFGYVAAFMYEGDAPLAERRAQALSLDRTLLAELLGREELRDLIDPAALADLELELQL
                     LTPERRVGTVDGLHDALRLLGDLTVAEATARAADPAALPGWLAELEGTRRALRLRIGG
                     EERWVAIEDAARFRDALGTAMPPGVPTAFLEPVADPVADLVSRFARTHGPFLAADVAR
                     RLGLGVAVVEQTLVRLEGAGRVVAGEFRPGGAGREWLDAEVLRRLRRRSLAALRKEVE
                     PVPQAALARFLAAWQGVAPVVPAAEGTGGSRPGSGRGQGRRPYPGGASGGVAALDAVG
                     RVVEQLQGVAVPASALERQVLATRLPGYEPGLLDQLCASGEVVWAGAGALGGDDGWVS
                     LYLAEQAPLLLPEPLPVELSPLAVAVHEALEDRGALFFRQLSDAVATSTGGGLGNDTE
                     LLFAIWELVWAGKVTNDTLAPLRALVSGAVRAPGRAPSGRPRAGRPRRPTMPSRLGPP
                     AGAGRWSLVPARSTDATRRLHAAAEQLLERHGVVTRGAVVAERIPGGFAGVYAVLKAM
                     EESGRCRRGYFVDGLGGAQFAVPGAVDRMRALVEPVERNPHPQTLVLAAADPANPYGA
                     ALAWPDRALGADSPAAERPGHRPGRKAGAVVVLVDGALVLYVERGGRTLLTFDNGPDH
                     LRRAADALALAVRDGALGRIAVERADGETVFDSPLAQALTAAGFRPTARGLRILGRPG
                     SASLGPGHGGAQPDAGR"
BASE COUNT          318 a         1126 c         1160 g          418 t
ORIGIN      
        1 gtcgtcgtgg cgcatcgagg agatcacccg cgaccgcgtt gtggtcaccc ctgctcccgg
       61 ccagcccggg aagatgccgt tctggcatgg ggacgggctg ggccggccct acgagctggg
      121 ccgggccatc ggcgccttcc tcggtgaggt cgacggctgg tccgacgagc gcctggccga
      181 ggagtgctcg ctcgacgacc gggccgtgcg caacctgcgc gcctatctgg ccgaggagcg
      241 cgaggtcact ggcgcggtcc cgaccgaccg ccagatcgtg atcgagcgct tccgcgacga
      301 gctgggcgac tggcgggtct gcgtccactc gccgttcggg gcgccgctgc atgccccctg
      361 ggcccaggcg atcgaagcgc gcgtgcgcga acgcctcggc ttggaggtcc agacgatgta
      421 ctccgacgac ggcatcgtgg tgcgcctgcc cgaggcggac gaggcgccgc cgtccgactc
      481 ggtcctgttc gagcctgagg aggtcgacga cctggtcgtc gggcaggtca gcggctcggc
      541 gctgttcgcc agccgtttcc gcgaatgcgc cgcccgcgcc ctgctgctgc cccggcgccg
      601 ccccggccgg cgtacccccc tgtggcagca gcgtcagcgc agcaccgggc tgctgcaggt
      661 cgccgccggc tacggctcct tcccgatcgt gctggagacc atgcgcgaat gcctccagga
      721 cgtcttcgac ctgccgggcc tggccgagct gatggccgcc gtgggccgtc gtgaggtccg
      781 cgtggtcgag gtcgacacgc cgttcccgtc gccgttcgcg agcagcctcc agttcggcta
      841 cgtagccgcg ttcatgtacg agggcgacgc cccgctcgcc gagcgccgcg cgcaggcgct
      901 gtcgctggac cgcaccctgc tggcggagct gctcggccgt gaagagctgc gcgacctgat
      961 cgatccagcc gccctcgccg acctggagct ggagctgcag ctgctcaccc cggagcggcg
     1021 cgtgggtacg gtcgacggtt tgcacgacgc gctgcgcctg ctcggcgacc tgacggtggc
     1081 ggaggcgacc gcccgcgccg ccgacccggc cgcgctgccc ggctggctgg ccgagctgga
     1141 ggggacccgc cgtgccctgc gcctgcggat cggcggcgag gaacgctggg tcgcgatcga
     1201 ggacgccgcc cgcttccgcg acgccctcgg caccgccatg ccgccggggg tgccgaccgc
     1261 cttcctggag ccggtcgccg atccggtcgc cgacctggtc agccgcttcg cccgcaccca
     1321 cgggccgttc ctggccgccg acgtggcccg ccggctgggt ctcggcgtcg ccgtggtgga
     1381 gcagaccctc gttcggctgg agggcgccgg acgggtcgtg gcaggggagt tccgccccgg
     1441 cggcgcgggg cgggaatggc tcgacgccga ggtgctgcgc cgcctgcggc gccgctcgct
     1501 ggccgccctg cgcaaggagg tcgagccagt cccgcaggcg gcgctggcgc gattccttgc
     1561 tgcctggcag ggcgtcgccc cagtcgtgcc agcggccgag ggcacggggg gctcgcggcc
     1621 cggttctggt cgggggcagg gccggcgtcc ctacccgggt ggcgccagtg gcggggtggc
     1681 cgccctggac gcggtcggcc gggtcgtcga gcagcttcag ggcgtggcgg tgcccgcctc
     1741 agcgctggag cggcaggtcc tcgccacgcg gctgcccggc tacgagcctg gcctgctcga
     1801 ccagctctgc gcctcgggcg aggtggtctg ggcgggtgcc ggcgcgctcg gcggcgacga
     1861 tggctgggtc agcctctacc tggccgaaca ggcgccgctg ctactgcccg agccgctccc
     1921 ggtcgagctg tcaccgctgg ctgtggcggt gcatgaggca ctggaggacc ggggcgcact
     1981 gttcttccgc cagctctccg acgcggtcgc caccagcacg ggcggtggcc tcggcaacga
     2041 caccgagttg ctcttcgcca tctgggagct ggtctgggcc gggaaggtca ccaacgacac
     2101 cctggcacct ctgcgggcgc tggtctccgg cgccgtccgc gcacccggcc gcgcaccctc
     2161 cgggcggcct cgggcgggtc ggccgcggcg gcccacgatg cccagccggt tgggccctcc
     2221 ggcaggcgcg ggccgctgga gccttgtccc ggcgcggtcc accgacgcca cccggcgcct
     2281 gcatgccgcc gctgagcagt tgctggagcg ccacggcgtg gttacccgcg gtgcggtcgt
     2341 cgccgagcgc attcccggcg gttttgctgg cgtctacgcg gttctgaagg ccatggagga
     2401 gtccggccgc tgccgccgcg gctacttcgt cgacggcctc ggcggcgccc agttcgccgt
     2461 cccgggcgcg gtcgaccgca tgcgtgccct ggtggaaccg gtagaacgca acccgcatcc
     2521 gcagacgctg gtgctggcgg ccgccgatcc cgccaacccg tacggggcgg cgctggcctg
     2581 gcctgaccgc gcgctgggtg ccgacagccc ggcggcggag cggccggggc accggccggg
     2641 gcgcaaggcg ggcgcggtgg tggtgctggt cgacggggca ctggtcctgt acgtggaacg
     2701 gggcgggcgc accctgctca ccttcgataa cggcccggac cacctgcggc gggccgccga
     2761 cgcgctcgcg ctggcggtcc gcgacggggc gctcgggcgg atcgcggtcg agcgggccga
     2821 cggcgagacg gtgttcgaca gccccctcgc ccaggccctg accgcggcgg gtttccgccc
     2881 gaccgcccgc ggcctgcgga tcctcggccg gccgggaagc gccagcctcg gccctggcca
     2941 cggcggagcg cagcccgatg ccggaaggtg acaccgtcca cctgctggcc gccaagctcg
     3001 acgccgcgct ggcgggacag cg
//
LOCUS       DASPYY010000161         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2134850, whole genome shotgun sequence.
ACCESSION   DASPYY010000161 DASPYY010000000
VERSION     DASPYY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134850"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VEP73_02390"
     CDS             complement(<1..433)
                     /locus_tag="VEP73_02390"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYT25321.1"
                     /translation="MRARRGRRPLGSPDARRAVLDAARELFAELGFERTTMRAVAARA
                     GVDPALIYHYFGDKDGLLFAALQPPVDEATVVAGLADAGDRTGEELVRRLVKLWEERP
                     EIREQMAAILRTGLSHDHASRLLRDILSSFILAALGDVLAGD"
     gene            complement(439..951)
                     /locus_tag="VEP73_02395"
     CDS             complement(439..951)
                     /locus_tag="VEP73_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25322.1"
                     /translation="MLVRELPLGAQRQMAFLAALAHSPGVIVLDEPTSGVDALARAAL
                     WDTIRAQAERGAGVLVTTHYMQEAQQCDRLLLMSDGRLVAEGSEADIIGSTTAVAVRT
                     GDWARAFAVLAAAGAPVMLAGRAIRVANADPAGLAEVLEAAGIQASLQPVPATIEERM
                     LMLSRQAKAS"
     gene            complement(1009..1731)
                     /locus_tag="VEP73_02400"
                     /pseudo
     CDS             complement(1009..1731)
                     /locus_tag="VEP73_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1728..>1892)
                     /locus_tag="VEP73_02405"
     CDS             complement(1728..>1892)
                     /locus_tag="VEP73_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25323.1"
                     /translation="APIASLWLPFVVLTAMAVVVFTGATLRFRRDLAPGRAASPAGEE
                     PAATETVGTA"
BASE COUNT          255 a          720 c          692 g          225 t
ORIGIN      
        1 ggtcgccggc caggacgtcg ccgagcgccg cgaggatgaa cgagctgagg atgtccctca
       61 ggagccggct ggcgtggtcg tgggacaggc ccgtccggag aatcgcggcc atctgctcgc
      121 ggatctccgg gcgctcctcc cacagcttga ctagccgcct gaccagctct tctccggtcc
      181 ggtcacccgc gtcggcgagg ccggcgacca ccgtggcctc atccaccggc ggctggagcg
      241 cggcgaacag gagcccgtcc ttgtcgccga agtagtgata gatgagcgcg gggtcgaccc
      301 cggcccgggc ggccacggcg cgcatggtgg tgcgctcgaa gccgagctcc gcgaacagtt
      361 cccgcgcggc gtccaggacg gcccggcgcg cgtccgggct gcccagcggg cgccggcctc
      421 tccgggcccg caccggcact aggaagcctt cgcctggcgg gacagcatca gcatccgctc
      481 ctcgatggtc gcggggaccg gctgcaggct ggcctggatc ccggcggcct cgagcacctc
      541 ggccagcccg gccgggtcgg cgttggccac ccggatcgcc cggcccgcga gcatgaccgg
      601 cgcacccgcg gcggccagga cggcgaaggc ccgcgcccag tccccggtgc ggaccgcgac
      661 ggcggtggtc gagccgatga tgtcggcctc gctgccctcg gcgaccagcc ggccgtccga
      721 catcagcagc agccggtcgc actgctgggc ctcctgcatg tagtgcgtcg tcaccaggac
      781 tccggcgccg cgctcggcct gcgcccggat cgtgtcccac aatgctgccc gggccagcgc
      841 gtcgacgccc gaggtcggtt cgtcgagcac gatcaccccg ggtgaatgcg ccagggcggc
      901 gaggaacgcc atctggcgct gcgctccgag cggcagctct cgcaccagca catcggcgtg
      961 ctccgtcagc ccgggcggca gatccgacgg ggtcgaaggg gcaccgccgg gccagtactc
     1021 atgccgtacg cggccccggc gccaggccca ttcggcccgg acgggctggc tggcctgggt
     1081 gatgtcgccc gcgaagtccg cgcgcacccg gtcgtagggt ccctgcacga gaacgcgccc
     1141 gcggtcgagg acgacgaggt gcccggcccg ttcggcctca tcgagatagg tggtcgacat
     1201 gagcacggcc gcgcccgacg ccgcggcctc cgacaccagc cgccacaggt cgatgcggct
     1261 caccgggtcg acgcccgtac tgggctcgtc caggatcagc aagtccggcc ggtgcacgat
     1321 cgccatgacg aagccgagct tgcggcgcat ccccccggac agctgcgcgg cgagccggcc
     1381 ggcggcggga gtcagcgccg cccggtccag cagctcggcg cggcgggccg cgagctcatc
     1441 gccggccagg ccgtagatgc ccgcggcgaa gtcgatgttc tgcgtcacgg tcagcgcggc
     1501 ccagctgccc gacgacgcgg ggaggtagcc caggcgctgc cgcggcggtg cttccaccgt
     1561 gccgtcgtcc agggatacct cgccggccag cgcgcgcagc agcgtcgact tgccggctcc
     1621 gtccccgccg accacggcga cgacggatcc ggcgggcacc tcgacggtga catcgtcgag
     1681 cgccaccgtg cgcccgaagc gcaccgtggc cgagcgcacc gagtacgtca cgcggttccc
     1741 accgtctcgg tcgcggccgg ctcctcgccg gccgggctgg cggcgcggcc cggggcgaga
     1801 tcccggcgga agcgcagcgt cgcgccggtg aacaccacga ccgccatcgc ggtgagcacg
     1861 acgaacggca gccacagcga ggcgatcggg gc
//
LOCUS       DASPYY010000162         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1994487, whole genome shotgun sequence.
ACCESSION   DASPYY010000162 DASPYY010000000
VERSION     DASPYY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994487"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1376
                     /locus_tag="VEP73_02410"
     CDS             171..1376
                     /locus_tag="VEP73_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018585666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25324.1"
                     /translation="MSASPRVPAARRLGLRDLVAAGAAGLATRRLRAALSALGVAIGI
                     AAIVAVLGISDSSKADLLAQLDRLGTNLLTVAPGQTFGGETATLPTQSAGMIRRIRPV
                     QQAAATATVSQTAVYRNDHIPPEETGGLSVAAADLRLPQAVGATLATGAWLNAATARY
                     PTTVLGAAAATQLGIDRVGSQVWLGGRWFTVVGILHPVTLAPELDRATLVGFPAAEAL
                     LGADGSPTTIYLRADPTQVTDVQGVLAATANPEHPNEVQVSRPSDALAAQAAAKSALN
                     SLLLGLGGVALLAGGVGIANVMVIGVLERRSEIGLRRALGATRGHVRVQFLVESLLLA
                     LLGGVGGVALGALVTAGYATHQHWQVVVPLTGVAAGVGAALALGAVAGLYLAVRAARL
                     SPTDALRTA"
BASE COUNT          184 a          639 c          536 g          190 t
ORIGIN      
        1 gtgctcgcgc tgctggagga gctcaacgcc gaaggcacca ccgtcgcggt catcacccac
       61 gaccgcgaca tctccgcacg cctgccacgc cgggtggagc tgcgcgacgg gcgcgtggtc
      121 gccgacacca gcacgccagg cgccgcgcac cagcagcagg gggtgacccg atgagcgcct
      181 ccccacgggt gcccgcggcc cgccggcttg gcctgcgcga cctggtcgcg gccggcgccg
      241 ccgggctcgc gacccggcgc ctgcgcgcgg cgctgtccgc gctgggcgtg gcgatcggta
      301 tcgccgcgat cgtcgcggtg ctcggcatct ccgactccag caaggcggac ctgctcgccc
      361 agctcgaccg gctcggcacc aacctgctca ccgtcgcccc cgggcagacc ttcggcgggg
      421 agaccgccac cctgcccacc cagtcggcgg ggatgatccg gcgcatccgc ccggtgcagc
      481 aggccgccgc caccgccacg gtgagccaga ccgcggtgta ccgcaacgac cacatcccgc
      541 ccgaggagac cggcgggctc agcgtcgccg ccgccgacct gcggctcccc caggcggtcg
      601 gcgccaccct ggccacgggc gcctggctca acgccgccac cgcgcgctac cccaccaccg
      661 tgctcggcgc ggccgccgcg acccagctcg gcatcgaccg ggtcggcagc caggtgtggc
      721 tcggcggccg ctggttcacc gtcgtcggca tcctccaccc ggtcacgctc gcgcccgagc
      781 tggaccgggc cacgctggtc ggcttccccg ccgccgaagc gctgctcggc gccgacggct
      841 cacccaccac catctacctg cgcgccgacc ccacccaggt caccgacgtc caaggcgtgc
      901 tcgccgccac cgccaacccc gagcacccca acgaggtcca ggtcagccgc ccgtcggacg
      961 cgctcgccgc ccaggcggcc gccaagagcg cgctcaactc gctgctgctc ggcctcggcg
     1021 gggtcgcgct cttggccggc ggggtcggca tcgccaacgt gatggtcatc ggcgtgctgg
     1081 agcgccgcag cgagatcggc ctgcgccgcg cgctcggcgc cacccgcggg cacgtccgcg
     1141 tgcagttcct ggtcgagtcg ctgctgctgg cgctgctcgg cggcgtcggc ggggtcgcgc
     1201 tcggcgcgct ggtcaccgcc ggctacgcga cccaccagca ctggcaggtg gtcgtgccgc
     1261 tgaccggcgt ggccgccggg gtcggcgcgg cgctggccct cggcgcggtc gccggcctgt
     1321 acctcgcggt ccgcgccgcc cgcctgtcgc ccaccgacgc gctacgcacg gcctgatcgg
     1381 cggaggcaca ccgaacggag gcacgcggct cggtggcccg tctcgaggcg ggcgtggccg
     1441 ttgtcgtggt gtctggaata taaccggtta tcggtcataa acacgcccat ccgacctgcg
     1501 ttccacgcgg ccgaccgata cgctgcgtcc ggcacgctcc agtggccgg
//
LOCUS       DASPYY010000163         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2557029, whole genome shotgun sequence.
ACCESSION   DASPYY010000163 DASPYY010000000
VERSION     DASPYY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557029"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="mreB"
                     /locus_tag="VEP73_02415"
     CDS             complement(<1..669)
                     /gene="mreB"
                     /locus_tag="VEP73_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HYT25325.1"
                     /translation="MARDLAIDLGTANTLVYVRGRGVQLDQPTVIALNERTGDILAMG
                     TEAYAMIGRTPAHIVAVRPLRGGAISDYDTTARLLRMLLRRVEARRIGRPKVVICVPS
                     AITPVERRAVQEAAVQAGARAAYLMEEPMAAAIGLGLPVHEPTGHLVVDVGGGTTEVA
                     CISLGGIVSARAIRVAGFDMDAAIVQYIRREYAVVIGERTSEDLKISLGSAVRPREDA
                     KVEVR"
     gene            complement(721..>1763)
                     /locus_tag="VEP73_02420"
     CDS             complement(721..>1763)
                     /locus_tag="VEP73_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25326.1"
                     /translation="IHPGARNLALRLEDDPSVDRVVGLAAHEPPLLGPKFEFVQASPG
                     DEGFAGALAEADAVVLFPLLDASDRDGGRRARLVEGVELAIGAARRAATVVLWSSGVV
                     YGAHPDNPVPIQEGQAVRPNPGFAPAEALAEVERVVLAAGEGPAPSAAPSGAPSAGRS
                     ATVLRAAGIWAPAWGTFLSRSLGAPVILGARGYDPPVQALHPDDATAALALAVTGGLS
                     GTYNVAPGDWVPARTVAGTAGRRRVELPERAAFAAAEQLWGLGLAGTRPGELPYHMHP
                     WVLDSSRLRAAGWAPAHSTAGALVDAARGLADGVLVGRVRVRRGDVYRGAAAAVAAFA
                     AVAVARRQGRRR"
BASE COUNT          213 a          692 c          665 g          193 t
ORIGIN      
        1 ccgcacctcg accttggcgt cctcgcgggg gcgcacggcc gagccgagcg agatcttcag
       61 gtcctcggag gtgcgctccc cgatgacgac ggcgtactcg cgccggatgt actggacgat
      121 cgcggcgtcc atgtcgaagc cggcgacccg gatcgccctg gcgctgacga tccccccgag
      181 cgagatgcag gccacctcgg tcgtcccgcc gccgacgtcg accaccagat gcccggtggg
      241 ctcgtggacg ggcagcccga ggccgatcgc ggcggccatc ggctcctcca tcaggtaggc
      301 ggcacgggcg ccggcctgca ccgcggcctc ctggacggcc cggcgctcga ccggggtgat
      361 cgccgacggg acgcagatga ccaccttcgg cctgccgatc cgtctcgcct ccacccgccg
      421 cagcagcatc ctgagcagcc gtgcggtggt gtcgtagtcg gagatggccc cgccgcgcag
      481 ggggcggacg gccacgatgt gggcgggcgt ccggccgatc atcgcgtagg cctcggtccc
      541 catggcgagg atgtcgccgg tgcgctcgtt gagggcgatg acggtcggct ggtcgagctg
      601 gacgccgcgg ccgcgcacgt acaccagcgt gttggccgtg ccgaggtcga tcgccaggtc
      661 gcgggccatg gccgggctag gctcccgcga gggcgctcgg ggccggaacg ccgcggcgtc
      721 tcaccgccgc cgcccctgcc gccgggccac ggccaccgcc gcgaacgcgg ccacggcggc
      781 ggccgccccc ctgtagacgt cgccgcgccg cacccggacc cggcccacca gcacgccgtc
      841 ggcgagtccg cgggccgcgt cgacgagcgc cccggcggtc gagtgggcgg gcgcccagcc
      901 tgccgcccgc agccggctcg agtcgagcac ccacgggtgc atgtggtacg ggagctctcc
      961 cggcctcgtc ccggcaagcc ccagtcccca gagctgctcg gcggctgcga aggcggcgcg
     1021 ctcgggcagc tccacccgcc tgcggccggc cgtgccggcc accgtccgtg caggcaccca
     1081 gtcccccggc gcgacgttgt aggtccctga caggccgccg gtgacggcga gagcgagggc
     1141 ggcggtcgcg tcgtcggggt gcagggcctg gaccggcggg tcgtacccgc gcgccccgag
     1201 gatcaccggc gcgcccagcg accgcgacag gaaggtgccc cacgcgggcg cccagatccc
     1261 ggcggcgcgc aggacggtcg ccgagcgccc cgccgagggc gcccccgagg gtgccgccga
     1321 gggcgccggc ccctcccccg ccgccagcac gacccgttcg acctcggcga gcgcctcggc
     1381 cggcgcgaag cccgggtttg gccggacggc ctggccctcc tggatcggga ccgggttgtc
     1441 ggggtgcgcg ccgtacacca ccccgctcga ccagagcacg accgtggcag cgcgccgggc
     1501 cgccccgatg gcgagctcga cgccctcgac cagccgggca cgccggccgc cgtcacggtc
     1561 gctcgcgtcg agcagcggga agagcacgac cgcgtcagcc tcggcgaggg cgccggcgaa
     1621 gccctcgtcc cccggggagg cctggacgaa ctcgaacttg gggccgagga ggggcggctc
     1681 gtgcgcggcc agcccgacga cccggtcgac cgacgggtcg tcctcgagcc ggagcgccag
     1741 gttcctcgca cctgggtgga tcg
//
LOCUS       DASPYY010000164         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2417116, whole genome shotgun sequence.
ACCESSION   DASPYY010000164 DASPYY010000000
VERSION     DASPYY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417116"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEP73_02425"
     CDS             <1..194
                     /locus_tag="VEP73_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25327.1"
                     /translation="TQATPSAEALVELALPRFHREEFTLPLDVTPMYLRRPDIDPAVE
                     RRLAAERAAAGRVSPVPGA"
     gene            197..763
                     /gene="rimI"
                     /locus_tag="VEP73_02430"
     CDS             197..763
                     /gene="rimI"
                     /locus_tag="VEP73_02430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYT25328.1"
                     /translation="MASLYPPPGHVGGSLPPLAVELVPMRRRHIGQVLEIERQVYPRP
                     WTMTLFLSEIVQRSTRFYVVARAHRRVVGYAGLMVFGDEAHVTNIAVDPQQHRRKIAA
                     RLLFALVTEARRRGATACTLEVRVANHAAQGLYHQFGFAPVGIRKNYYAETGEDALIM
                     WAEGLQGGAYTARLAELARRIPEPREVG"
     gene            766..1827
                     /gene="tsaD"
                     /locus_tag="VEP73_02435"
     CDS             766..1827
                     /gene="tsaD"
                     /locus_tag="VEP73_02435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYT25329.1"
                     /translation="MLVLGIETSCDETGVALVEDGSRVRSNVIADQRTLHDRYGGVVP
                     EVASRAHVDLLHPAVEQALAFGGARLNDVDGFAVTVGPGLAGSLLVGVAAAKAYALAL
                     DRPLIGVNHLIAHVYANVIEHGPLPLPAAVFVVSGGHTSLLVMGSDGAFRVLGQTIDD
                     AAGEAYDKVARVLGLGYPGGPILDRLAAGGDPGAVRFPRALLDDGTDDFSLSGLKTAV
                     LRHVRAVARSGGQLRPADVAASFQEAVVDVQVSKVVGAARREGLDTVLLAGGVAANSR
                     LRSRLRDEARKAGIRVLVPSPALCTDNGAMVACAGSLALERGERSPLDVGADPNLPIV
                     PGVGRRAVRRASARPRQAD"
BASE COUNT          223 a          736 c          676 g          253 t
ORIGIN      
        1 gcacccaggc gacgccgtcg gccgaggccc tggtcgagct ggcccttccc cgtttccacc
       61 gtgaggagtt caccctgccc ctcgacgtga cccccatgta cctgcgccga cccgatatcg
      121 accccgccgt cgagcgccgc ctggccgcgg agcgggccgc cgccggccgg gtctcgccgg
      181 tcccgggagc ctgaccttgg cctcgctgta cccgccgccg ggccacgtcg gaggctcgct
      241 cccgccgctg gcggtcgagc tcgtccccat gcgccgacgt cacatcggcc aggtgctcga
      301 gatcgagcgc caggtctacc cgcggccgtg gaccatgacg ctgttccttt cggagatcgt
      361 gcagcgctcg acccgcttct acgtggtcgc ccgggcccac cggcgcgtgg tgggctacgc
      421 cggcctgatg gtcttcgggg acgaggcgca cgtcaccaac atcgcggtcg acccccagca
      481 gcaccggcgc aagatcgcgg cacggctgct gttcgccctg gtcaccgagg cgaggcgcag
      541 gggcgcgacc gcctgcacgc tcgaggtccg ggtggccaat cacgccgccc agggcctcta
      601 ccaccagttc ggcttcgctc ccgtcgggat ccgcaagaac tactacgccg agaccggcga
      661 ggacgcgctc atcatgtggg ccgagggcct ccagggcggc gcgtacacgg cccgcctggc
      721 cgagctggcg cgccggatcc ccgagccgcg agaggtgggc taggcgtgct ggtcctcggg
      781 atcgagacga gctgcgacga gaccggcgtc gcgctggtgg aggacggcag ccgcgtgcgc
      841 tccaacgtga tcgccgacca gcggaccctg cacgaccgct acggcggcgt cgtccccgag
      901 gtcgcctccc gggcccacgt cgacctgctg cacccggcgg tcgagcaggc cctggcgttc
      961 ggcggcgccc ggctcaatga cgtcgacggc ttcgcggtca cggtcggccc ggggctggcc
     1021 ggctcgctgc tcgtcggcgt ggcggctgcc aaggcgtacg cgctcgccct cgaccgcccc
     1081 ctcatcgggg tcaaccacct gatcgcccac gtctacgcca acgtcatcga gcacggcccg
     1141 ctaccactgc ccgccgccgt cttcgtggtc tcgggcgggc acacgagcct gctggtcatg
     1201 gggtccgacg gcgcgttcag ggtcctcggc cagacgatcg acgacgccgc cggcgaggcg
     1261 tacgacaagg tggcgcgggt gctcgggctc ggctaccccg gcggcccgat cctggaccgg
     1321 ctggccgccg ggggcgaccc gggcgcggtc cggttccccc gtgccctgct cgacgacggg
     1381 accgacgact tctcgctgtc cggcctgaag acggccgtgc tccgtcacgt gcgggccgtg
     1441 gcgcggtccg gcgggcagct gcggcccgcc gacgtggccg cctccttcca ggaggccgtg
     1501 gtcgacgtcc aggtgagcaa ggtcgtgggc gcggcccggc gggaggggct cgacaccgtg
     1561 ctgctcgccg gcggggtggc ggccaactcg cggctccggt cccggctgcg ggacgaggcc
     1621 cgcaaggcgg gcatccgcgt cctcgtcccc tcgccggcgc tgtgcaccga caacggcgcc
     1681 atggtcgcct gcgccggctc gctcgcgctc gagcgggggg agcgcagccc gctcgacgtc
     1741 ggtgccgacc cgaacctgcc tatcgtcccc ggggtcggcc gccgcgccgt ccgccgcgcg
     1801 agcgcccgcc cccgccaggc cgactagcac tcgccaatgg cgagtgctaa tatccgcggc
     1861 agcgccagcc acggcccgcg gcacgagg
//
LOCUS       DASPYY010000165         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_169871, whole genome shotgun sequence.
ACCESSION   DASPYY010000165 DASPYY010000000
VERSION     DASPYY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169871"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VEP73_02440"
     CDS             <1..1240
                     /locus_tag="VEP73_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25330.1"
                     /translation="SRGSFVGLLGLFWSRHHRLDQEQEALLGLAGNQIAGALAAALRY
                     EEAERAAGEARAVFYAIADGVLLTDPAGRVTAMNRAMELLSGWTEAEVQGMAYAEVLP
                     VTEEGGGHIRPEARPLARAIASGEPASDQGYGVDLVTRDGRKVPVAVSSAPILDPRGR
                     VVGGVEVVRDVSREREIDEVKSAIISTVSHELRTPLTLIHGFAELLVLRDMPVERQRA
                     AAEEILEASRRLARLIDDLLSASRMESGRLVLDPRPLDLAGMVERILSPFRAMAARHV
                     IRAEVAGGLPVVWGDPDKVEQILTNLVGNAIKYSPGGGEVVVSVTPLPEAGQVEVSVR
                     DQGIGMSPREMAQLFEKFYRVDRDEVRRAGGTGLGLYITKRLVELHGGRIRAESWLGT
                     GSVFSFTLPGRNDLAGGGGR"
     gene            1237..>1652
                     /locus_tag="VEP73_02445"
     CDS             1237..>1652
                     /locus_tag="VEP73_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT25331.1"
                     /translation="MKLLIADDEPSVRALVHVTLEGDEHTILEAADGVEALEVTRSER
                     PGLVLLDIMMPRLDGLTVCRAIKSDPATSGTVVVMLTAQAQGRDRDLGLAAGADDYFT
                     KPFSPLALLNMVERVRETRPGRAQGDRGVGDRAPGRP"
BASE COUNT          227 a          585 c          621 g          219 t
ORIGIN      
        1 gtcccggggc tcgttcgtcg gcctgctcgg cctcttctgg tcccggcatc accggctcga
       61 ccaggagcag gaggcgctgc tcggcctggc cggcaaccag atcgccgggg cgctcgccgc
      121 cgcgctgcgc tacgaggagg cggagcgggc cgccggagag gcccgcgcgg tgttctacgc
      181 gatcgcggac ggcgtgctgc tcaccgaccc ggcaggccgc gtcacggcga tgaaccgggc
      241 catggagctg ctcagcggct ggaccgaggc cgaggtgcag ggcatggcct acgcggaggt
      301 cctccctgtc accgaggagg gcggcggcca catccggccc gaggcgcgcc ccctcgcgag
      361 ggccatcgcc agcggcgagc cagcgagcga ccagggctac ggcgtcgacc tcgtcacccg
      421 cgacggccgc aaggtgcccg tggcggtcag ctcggccccc atcctcgacc cccgcggccg
      481 cgtggtcggc ggcgtcgagg tggtcaggga cgtctcccgc gagcgcgaga tcgacgaggt
      541 gaagtcggcc atcatctcga ccgtctccca tgagctgcgg acgccgctca ccctgatcca
      601 cgggttcgcc gagctgctcg tgctgcgcga catgccggtc gagcggcagc gggcggcggc
      661 cgaggagatc ctcgaggcca gccgcaggct cgcccgcctc atcgacgacc tgctgagcgc
      721 gtcgcgcatg gagtccggcc gcctggtcct cgacccccgc ccgctcgacc tggccggcat
      781 ggtggagcgc atcctctcgc cgttccgggc catggcggcc aggcatgtca tcagggccga
      841 ggtcgccggc gggctgcccg tcgtctgggg cgaccccgac aaggtggagc agatattgac
      901 caacctcgtc ggcaatgcga tcaaatactc ccccggaggg ggggaggtgg tcgtgtcggt
      961 cacgccactg cctgaagcgg gccaggtcga ggtgagcgtc cgcgaccagg ggatcggcat
     1021 gagcccgcgc gagatggcgc agctgttcga gaagttctac cgggtcgacc gggacgaggt
     1081 ccgccgggcg ggcgggaccg gcctcggcct gtacatcacc aagcggctgg tcgagctgca
     1141 cggtgggaga atccgggccg agtcctggct cgggacgggg tcggtcttct ccttcacgct
     1201 cccgggccgc aacgacctgg cgggaggggg gggccgatga agctcttgat cgccgacgac
     1261 gagccgtcgg tgcgggcgct ggtgcacgtc accctcgagg gcgacgagca caccatcctg
     1321 gaggcggccg acggcgtcga ggcgctcgag gtgacgcgca gcgagcggcc cggcctggtc
     1381 ctgctcgaca tcatgatgcc gagacttgac ggcctcacgg tgtgccgggc gatcaagtcc
     1441 gacccggcca cgagcggcac cgtggtcgtc atgctcacgg cccaggcgca gggccgggac
     1501 cgggacctgg gcctggcggc cggcgccgac gactacttca ccaagccgtt ctcgccactg
     1561 gcgctgctca acatggtcga gcgggtgcgc gagacccgtc ccggccgcgc tcagggcgat
     1621 cgcggcgtag gggaccgcgc gccgggccgc cc
//
LOCUS       DASPYY010000166         6674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1856387, whole genome shotgun sequence.
ACCESSION   DASPYY010000166 DASPYY010000000
VERSION     DASPYY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856387"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3345)
                     /locus_tag="VEP73_02450"
     CDS             complement(<1..3345)
                     /locus_tag="VEP73_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25332.1"
                     /translation="MGFVYEAVRAPIAVLEVVTETGLGGLTATLPAEFVGAVQDAGAG
                     VLTLNLAALPAGRCQVTLVLVDREERRSVPFAFGLDVPGGQHGAAGPVLRAVRAEQER
                     LTRPDGDDLVNAWFAVACERGGSALAGVYVRVSAPDGRQAGFAAPLPPQAGELDAARL
                     RLIVFGATEALGAYRVAVTVLDADGNASETQEAAVELVEAGGGQGPRVDGFEPVVAAA
                     GETMTVHGGGFDGEELAVLVGDVRADLLAVAPDRLTLLAPPVDQPAQVAVVTRDGWGT
                     SQVDFVPRIAVRVVPERFELPEGDAIQLTAVVSGTRDSGAGWRLDDAAGEVSPSGVYT
                     APPFSGPQTVRVTATSSADPQAVATATGSLVARPPTRGPARLGLLGGTVLSEDGGSTL
                     VVPVDALEEPAVVEIQRVAADLADEAVDAEAIIVAAARVLPDGLELRRPAELTIPLRT
                     YLEPDASIALQALDVRTNRWTDLDELGLVIDGDRLRIEQTKFYGGVRALHHLGPLPRS
                     FAPAISGVAPPALEEGATAAVLVTGKNFVPGATTLTVLDAVSGQVEPRVDVRTIFVTA
                     DGTRLGVTLKAGVMTDLAAGLTRPVTLRVSTPGGSASASLTVVGHDELDLPSGSRTLF
                     FSQVFSRLTVGPGARLTVLNRVPAIDITVLERAQIDARFEASGGVLEVGPGTGGTGTR
                     GTAGGGGGAGGVPSGGATMPGGGGAGGAGGANGATPGAAGGAGLPSPATGAGGGGGPG
                     GAGGGGGPFPHVADDGVPGGAASPFPPLPGVVPALTPGPGGGGGGGGGGEGWLSTNTG
                     GGGGGGGAGGGALRLSAGEELVINGDVFALGGNGGGGAYPYPYSPPAPTFLISAGRGA
                     GGGGGAGGALQLHGARWQSGFVLALGGESFRLPIYTSGPFPVDIRTAFERLLDQPATG
                     RIRFDGPRPGQQVVPAPFSGPDLDSCFNLVSPSPQFQLKGHGTAGTLQVRVRDRLGNE
                     QVAFPPAVDPVTGFTVAVPLYDGFNDIQAEFVLFGPPDVVWAVSAPVRSRTVLYLPGT
                     TPSFAFACSISPATVTVPTERTVSFAVTVTGTPLTAVTWSVDGGAANGAVSQAGAYTA
                     PCAAPSAPATVRVRSALDPSKSCTAQV"
     gene            3562..3903
                     /locus_tag="VEP73_02455"
     CDS             3562..3903
                     /locus_tag="VEP73_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25333.1"
                     /translation="MAFGLRGKERAKWDPELLDAPGKFYLQARLQGLTGPRSARGFWI
                     PTEQALQTYGHVIPGMDRDAAATVADLILRGTQKGSGTVVPDPSANKTASNGPPSDTP
                     GKEVKDERPAQ"
     gene            4186..4416
                     /locus_tag="VEP73_02460"
     CDS             4186..4416
                     /locus_tag="VEP73_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HYT25334.1"
                     /translation="MQRTRTVTTDTYFYTAEVAQLLRVSPKTVSRWAKQGKLPFLRTV
                     GGHRRYPETEIRALIAALTEPVVPLRGDADAE"
     gene            4406..5182
                     /locus_tag="VEP73_02465"
     CDS             4406..5182
                     /locus_tag="VEP73_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25335.1"
                     /translation="MRSEPPMGGPLRVAARAGTLTLLGLTASAGVVIGRTGRPSPTTA
                     APAPVTMTPAAARVGSPCARVWHRHWSARVCASVRVGRDRLGNQCVRASATARGWRWW
                     RDARGHWRHRRRALRFAMTVRLCGPGFCQATRHRTGWARRLGIRTGWRVVVDPMPAWA
                     RARVVVTFPDGARRVVTATSAPLWAAALGAVAPARTQHQELALALAPRRDGDRVAGPR
                     RSRRPACLVCLAKPSCVQRPPSTGFGSWAPPAASPGRSGC"
     gene            5140..>6674
                     /gene="mobF"
                     /locus_tag="VEP73_02470"
     CDS             5140..>6674
                     /gene="mobF"
                     /locus_tag="VEP73_02470"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HYT25336.1"
                     /translation="MGTTGGLTGAVGMLTARKLTGAGGLWAYLDHGGGEHDDRRAEYY
                     LGGATPHQVWGAGAERLGLDVLDRERFDRLAAGCHPETDERLVQTQQGTHIPGVDLVF
                     SPPKSVSVALVDATPAERAALEAAHLAATRAGMGMLEERAAVTRPTRDGKTVRVPGGL
                     VAGLFTHHTARPTTETVAEGRPPDPHLHTHGFVFSLAFSEGKWRSVDSYGLFRTRKLA
                     EAAYQVELAAQLRARGWQVETRTLRGGARVTELAGHDPAANELFSSRHREIEDLTRRW
                     EARYHRPPTRLERWRLSHDNRLAKDDRHGRQPQWAAYQRVLDAHGLTRHRLHAGQGGV
                     QAPLEEREAAVRAALLAPDGLTRQDAIFHHNDIPSAVLEASVGLLSLEEARDFLERFE
                     AGPDLVLVDEARGYLTTRAQLDREAAIADMVTAKREERPAAPAAAQVERAAARAKHPL
                     TQEQRAAVDHLLDPERGMGLLLGWAGTGKTTTMQVVVDATRQAGGKVVVVATSGDTAQ
                     RTAR"
BASE COUNT          917 a         2569 c         2397 g          791 t
ORIGIN      
        1 gacctgcgcg gtgcacgact tggacgggtc gagcgccgag cggacccgta ccgttgccgg
       61 tgccgagggt gcggcgcagg gtgccgtgta cgccccggcc tggctgacgg cgccgttggc
      121 ggcgccaccg tccaccgacc aggtgacggc ggtcagtggc gtgcccgtga cggtcacagc
      181 gaagctgacg gtccgctcgg tgggcacggt caccgtcgcc gggctgatcg agcaggcgaa
      241 cgcgaacgac ggcgtggtgc ccggcaggta gagcaccgta cgagagcgga ccggcgctga
      301 gacggcccag acgacatcgg gcgggccgaa caggacgaac tcggcctgga tgtcgttgaa
      361 cccgtcgtac agcgggaccg ccacggtgaa cccggtcacg gggtctaccg ccggggggaa
      421 ggcgacctgc tcgttgccca ggcggtccct gacgcggacc tgcagggtac cggcggtccc
      481 atgccccttg agttggaact gtgggctggg agaaacgagg ttgaagcagg agtcaaggtc
      541 cggcccggag aacggcgccg gtacgacctg ctggccgggt cgcggcccgt cgaatcggat
      601 gcggccggtg gccggctggt ccagcaggcg ctcgaaggct gtgcggatgt cgactgggaa
      661 gggtcccgag gtgtagatgg gcaagcggaa gctttcgccg ccaagggcga ggacgaaccc
      721 ggactgccag cgggccccgt ggagctggag cgctcccccg gcgccgccgc caccgccggc
      781 gcctctgccc gccgagatca ggaaggtggg cgcgggtggg ctgtaggggt acgggtaggc
      841 tcccccgccg ccgttgccgc cgagggcgaa cacgtccccg ttgatcacca gctcctcgcc
      901 ggctgacagg cgcagcgcgc cgccgcccgc gccaccaccc ccgccgcccc cgccggtgtt
      961 ggtgctcaac cagccctcac cgccgccgcc tccgccgccg ccgccgcccg gccccggcgt
     1021 cagggcgggt accaccccgg gcagcggggg gaacggggac gcggcgccgc cgggcacgcc
     1081 gtcgtcggcg acgtggggga aggggccgcc gcctccgcca gccccacccg gcccaccacc
     1141 gccgccggcc ccggtcgcgg gggatggcaa cccggcgccg ccagccgcgc cgggcgtggc
     1201 gccgtttgcg ccgccggccc cacccgcgcc gccgccaccg ggcatagtgg cgccgccgct
     1261 cggcaccccg ccggcgccgc ccccgccgcc tgcggtgccg cgtgtgccgg tcccgcccgt
     1321 tcccggaccc acttccagga cgccgccgct ggcctcgaag cgcgcgtcga tctgtgcccg
     1381 ctccagcacc gtgatgtcga tcgcgggtac ccggttcaag acggtgagcc gcgcccccgg
     1441 gccgacggtc agcctggaga agacctggga gaagaagagc gtccgcgagc cggatggaag
     1501 gtcgagctcg tcgtgaccga cgaccgtgag cgaggcgctg gccgagccgc ccggggtcga
     1561 gacgcgcagg gtcactggcc gcgtcagccc cgcggcgagg tcggtcatca cgccggcctt
     1621 gagcgtgacg ccgagcctgg tcccatcggc ggtcacgaag atcgtgcgga cgtcgacgcg
     1681 aggctcgacc tggcccgaga cggcgtcgag cacggtcagc gtggtcgccc cggggacgaa
     1741 gttcttgccg gtgaccagca ccgccgcggt cgcgccctcc tcaagggccg gcggcgcaac
     1801 cccgctgatc gccggcgcga acgacctcgg cagcgggcca aggtgatgga gcgcccggac
     1861 gcctccgtag aacttggtct gttcgatgcg cagccggtcg ccgtcgatca ccaagccgag
     1921 ctcgtcgagg tccgtccatc tgttggtgcg cacgtcgagc gcttgaagcg cgatcgaggc
     1981 gtccggctcg aggtaggtgc gaagcgggat cgtcagctcg gccggcctcc gaagctccag
     2041 gccgtcagga aggaccctgg ccgccgcgac gatgatcgcc tccgcgtcga ccgcctcgtc
     2101 ggccaggtcc gccgcgaccc tttggatctc gaccacggcc ggctcctcga gggcgtccac
     2161 cgggacgacc agcgtcgagc cgccgtcctc ggacaacacc gtcccgccga gcagccccaa
     2221 gcgcgccggc cccctggtcg gcgggcgggc gaccagcgac ccggtcgcgg tggccaccgc
     2281 ctgcgggtcg gccgaactgg tcgccgtgac gcgcacggtc tgcggcccac tgaagggggg
     2341 cgcggtgtac accccggacg gcgacacctc gcccgcggcg tcgtccaacc gccagccggc
     2401 gccggaatcc cgtgtgccgc tgacgaccgc ggtgagctgg atcgcgtcgc cctccggcag
     2461 ctcgaaccgt tcaggcacca cccgcacagc gatcctcggg acaaagtcca cctggctggt
     2521 gccccagccg tcgcgcgtga ccacggcgac ctgggctggc tggtcgacgg gcggggcgag
     2581 gagcgtgagg cgatcgggag cgacggccag caggtcggcg cggacgtcgc cgaccagcac
     2641 ggcgagctcc tccccgtcga agccaccccc atggacggtc atcgtctccc ccgccgccgc
     2701 aacaaccggc tcgaagccgt cgacccgcgg accctgcccc ccgccggcct cgaccaactc
     2761 gacggcggcc tcctgggtct cgctcgcgtt gccgtcggcg tcaagaaccg tcaccgccac
     2821 gcgataggcg ccaagcgcct cggtggctcc gaagacgatc aggcgcagcc gggccgcgtc
     2881 gagctccccc gcctgcggcg gcagcggcgc ggcgaagcct gcctggcgac catccggggc
     2941 gctgacgcgc acgtacacgc cggcaagcgc cgacccgccg cgctcgcagg cgaccgcgaa
     3001 ccaggcgttc accaagtcgt cgccatccgg gcgcgtgagc cgctcctgct cggcccgtac
     3061 cgcccggagc accgggcccg cagcgccgtg ctggccgccc ggcacgtcca gcccgaaggc
     3121 gaacggcacg ctacggcgct cctcgcggtc gacgagcacc aacgtcacct ggcagcgtcc
     3181 agcggggagc gccgcgaggt tcagcgtcag cacgccggcg ccggcgtcct gcacggcacc
     3241 gacaaactcg gccggcagcg tggcggtgag accgcccagc ccggtctccg taacgacctc
     3301 gagtacggcg atcggggcgc ggacggcctc gtagacgaac cccacctcga cagcgccccg
     3361 atccgccggc tgtggcacat caacgtcgtg gatcctcggt cggcggtcgt ctggtacgcc
     3421 agcgacgcgt cgctccatgc ggcaccccca agaacgtcag gccggcccaa agcctaaggt
     3481 ctgcccgccg aatcgtgaag ggaccaacga aacgccatct gctctccacc tcaccgcagc
     3541 gaagacctcg acgagctgcc gttggcgttc gggctgcgcg gcaaggaacg tgccaagtgg
     3601 gaccccgaac tcctggacgc cccggggaag ttctatctgc aggcccgcct gcagggcctc
     3661 accggcccgc ggtcagcccg ggggttttgg atcccgaccg agcaggccct gcagacctac
     3721 gggcacgtca tccccggcat ggaccgtgac gccgcggcga ccgtcgccga cctgatcctg
     3781 cgcggcacgc agaaggggtc cggcaccgtg gtgccggacc cctccgctaa caaaacggct
     3841 agcaacggcc cgccgagcga cacccctggg aaggaggtga aggacgaacg ccctgctcag
     3901 tagtggtggc gggggaggga tttgaaccct cgacctctgg gttatgagcc gttcaagcgg
     3961 catcctgagt agtcccgccc aagcccatca caccgacgct gaccagcggt ttcaccgccg
     4021 ctgcaagtgt cactcgatcc cacgctctcc cggcgggttc ccacctgtcc cggtgtcaga
     4081 tccgctgtca tccgggcacc gccccgggct ccttggccgt ttcatccccg gccgcgagcg
     4141 agtcgccatc gaccgccgcg gccccacgcc agggagccac gccagatgca gcgcacgcgc
     4201 accgtcacga ccgacaccta cttctacacc gctgaggtcg cccagctcct ccgggtctcg
     4261 ccgaagaccg tcagccgctg ggccaagcag ggcaagctcc ccttcctgcg gacggtcggc
     4321 ggccaccgcc gctaccccga gaccgagatc cgcgcgctca tcgccgcgct gaccgagccg
     4381 gtcgtccccc tgcggggtga cgccgatgcg gagtgagccc cccatgggcg gtccgctccg
     4441 ggtcgccgcc cgcgccggca cgctcaccct cctcgggctg accgcgagcg ccggcgtcgt
     4501 gatcggacgc accggacggc cgtcgccgac gaccgcggca ccggcgccgg tcaccatgac
     4561 gccggcagcc gcccgcgtcg gctccccgtg cgcccgggtg tggcatcggc actggtccgc
     4621 ccgggtctgc gcctccgtcc gcgtcgggcg cgaccggctc ggcaaccagt gcgtccgcgc
     4681 cagcgcgacc gcgcggggct ggcgctggtg gcgcgacgcc cgcggccact ggcgccaccg
     4741 gcgccgcgcg ctccggttcg cgatgacggt gcggctgtgc gggcctggct tctgccaggc
     4801 cacccgccac cgcaccggct gggcgaggcg gcttgggatc cggaccggtt ggcgggtggt
     4861 ggtcgacccc atgcccgcgt gggcgcgtgc ccgggtcgtg gtgaccttcc ccgacggcgc
     4921 ccgccgggtg gtcacggcca cgtcggcgcc gctgtgggcc gccgcgctcg gggccgtggc
     4981 gcccgcacgg acccagcatc aggagctcgc cctcgctctc gccccgcggc gcgacggcga
     5041 ccgggtcgct gggccgcggc gcagccgccg gccagcctgc ttagtctgct tggcgaagcc
     5101 gagctgcgta cagcgtccgc cttcaacggg gttcggatcg tgggcaccac cggcggcctc
     5161 accggggcgg tcgggatgct gacggccagg aagctcaccg gcgccggggg gctgtgggcc
     5221 tacctggacc acggcggcgg ggagcacgac gaccgcaggg cggagtacta cctgggcggc
     5281 gcgacgccgc atcaggtctg gggtgctggc gccgagcggc ttggcctgga cgtgctcgac
     5341 cgggagcggt ttgaccggct ggccgccggc tgccacccgg agaccgacga gcggctcgtg
     5401 cagacccagc aaggcaccca catccctggc gtggacctgg tgttcagccc gccgaagtcg
     5461 gtgtcggtcg cgctggtcga cgccaccccc gccgagcggg ccgcgctcga ggccgcgcac
     5521 ctggccgcca cccgcgccgg catgggcatg ctcgaggagc gcgccgcggt cacccggccg
     5581 acccgcgacg gcaagacagt ccgggtgccc ggggggctgg tcgccgggct gttcacccac
     5641 cacaccgcgc ggccgaccac cgagacggtc gccgaggggc ggccgccgga tccgcacctg
     5701 cacacccacg ggttcgtgtt ctcgctggcc ttctcggagg gcaagtggcg gtcggtcgac
     5761 tcctatgggc tgtttcgcac ccgcaagctc gccgaggcgg cctaccaggt ggagctcgcc
     5821 gcgcagctcc gcgcgcgcgg ctggcaggtg gagacgcgca ctctgcgcgg cggggcgcgg
     5881 gtcacggagc tcgccggcca cgacccggcc gccaacgagc tgttcagctc ccggcaccgc
     5941 gagatcgagg acctgacccg ccggtgggag gcgcgctacc accgcccccc gactcggctc
     6001 gaacgctggc ggctgtcgca cgacaaccgg ctcgccaagg acgaccggca cggccgccag
     6061 ccccagtggg ccgcctacca gcgggtcctg gacgcccacg ggctcacccg ccaccggctc
     6121 cacgccggcc agggcggcgt gcaggcgcca ctcgaggagc gcgaggcggc ggtccgcgcc
     6181 gcgctgctcg ccccggacgg gctgacccgc caggacgcga tcttccacca caacgacatc
     6241 cccagcgccg tgctcgaggc ctccgtcggg ctgctctcgc tcgaggaggc ccgcgatttc
     6301 ctggagcggt tcgaggcggg cccggacctg gtgctggtcg acgaggcgcg tggctacctc
     6361 accacccgcg cgcagctgga ccgcgaggcc gccatcgccg acatggtgac ggcgaagcgc
     6421 gaggagcggc cggccgcgcc ggccgcggcg caggtcgagc gcgccgcggc ccgcgctaag
     6481 catcccctca cacaagagca gcgcgccgcg gtcgaccacc tccttgaccc cgagcggggc
     6541 atggggctgc tgctcggctg ggccgggacc ggcaagacga ccaccatgca ggtcgtcgtc
     6601 gacgcgaccc gccaagccgg cggcaaggtc gtcgtcgtgg ccaccagcgg cgacaccgcg
     6661 cagcgcaccg cccg
//
LOCUS       DASPYY010000167         6147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1856608, whole genome shotgun sequence.
ACCESSION   DASPYY010000167 DASPYY010000000
VERSION     DASPYY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856608"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VEP73_02475"
     CDS             <1..837
                     /locus_tag="VEP73_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT25337.1"
                     /translation="VGGDAVLRPPGPRAPGGVAYESWAFANPAGDRVHGFLAAPAGPG
                     PHPTVLLVHGGPHHHDADSFSPEVQAWVDHGWAVGLVNYRGSTGYGKAWQDVLEGDPG
                     RPEVEDVVAGRDDLVARGVADPDRVVIAGASWGGYVTLQTIGTAPDGWRAALAVVPVA
                     DYVSAYADESEGLQALDRSLFGGGPEEKPELFAERSPITHAGEVKVPVLLMVGENDTR
                     CPLQQVLNYAERLTELGKEFELDRFDAGHGALVVDERIRQAEVELDFAARHVPGVAQP
                     EA"
     gene            complement(843..2279)
                     /locus_tag="VEP73_02480"
     CDS             complement(843..2279)
                     /locus_tag="VEP73_02480"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYT25338.1"
                     /translation="MQEVTRTAGPRLDAAAVEHLRGLTAAWQLIADLSFADLLLFAPV
                     EGGESFRIVGQLRPYTARTLYPADLIGDVVEPGWQPYVERAWREGRRLRSPEPVFIDA
                     EPVRVEAVPVRHEDDVVAVMSVEAADQPDRPTGRLETAYLRAADALLEMVATGWFPFQ
                     EGHELISSPRVGDGLVMLDSSGRVEFASPNATSAFRRMGTNALPLGQPVPGRVGVLVA
                     ESMSSRRPVEADVELGGAVTHVRVVPLLHGSGARRGALALCRELTELRGRERTISRRE
                     ATIREVHHRVKNNLQTVASLLRLQARRLGDHPMAVAALEESVRRIASIALVHETLTEE
                     LEGGADMADVARRVVRMLESSLGREDVRVTLVAGSVRADAAIATPLAVALTELVQNAI
                     EHGLGEGPGTVTVTLRGGDGKPIELEVADDGPGPVAEPRPRTGGGLGLRLVRALVEEE
                     LGGRFTLVLRPGQGSLAVVAVPPGPARE"
     gene            complement(2305..2748)
                     /locus_tag="VEP73_02485"
     CDS             complement(2305..2748)
                     /locus_tag="VEP73_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYT25339.1"
                     /translation="MPRPWQVAGIAAALFGIVTGSALWLNGTAHSSRAHDRIVVITIT
                     VHHSHFQPAEVAVEAGSTVRFVLRNTDPIDHEFILGDEAVQELHEKGTGAHHDAPGQV
                     SVPAGTERTTTVTLNWTGAGRLEYACHLPGHYAYGMRGVVVVRPG"
     gene            complement(2813..3319)
                     /locus_tag="VEP73_02490"
     CDS             complement(2813..3319)
                     /locus_tag="VEP73_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25340.1"
                     /translation="MLLDLFLPGVDGIGVLAQIKQDRPATEVLMLTSSQDDEHLLAAV
                     HAGALAYLPKTAGVDQVVTSVRAAARGESVLEPRIAARLVREVREAVVRRRPLDQLSP
                     RELEVLAALARGRSNRQIARTLTIGEETVKAHVSSILAKLGLADRTQAAIFGLQQRLV
                     PLDEALDE"
     gene            complement(3323..4521)
                     /locus_tag="VEP73_02495"
                     /pseudo
     CDS             complement(3323..4521)
                     /locus_tag="VEP73_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018585666.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4518..5257)
                     /locus_tag="VEP73_02500"
                     /pseudo
     CDS             complement(4518..5257)
                     /locus_tag="VEP73_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(5254..>6147)
                     /locus_tag="VEP73_02505"
     CDS             complement(5254..>6147)
                     /locus_tag="VEP73_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HYT25341.1"
                     /translation="ILTRLPAAGTVVGRGQALYEVDGHLVPLWYGTRPAWRTFGLGMT
                     DGPDVRQLEANLVALGFDPDRAITVDRHYSWATAAAVKRWQLAIGRSQTGAVALGQIM
                     FLPGPIRVTTLSATVGAPLGAGTAILTATSTRPLVTVALDPAVQQLVRRGDRVLVTLP
                     DGGTIPGTVTTVGRVAVQPATGTGQDGQDQSQGGNGNGAGQATVSVTVGLADPRAAAD
                     LDQAPVQVAITTQAHKGVLAVPIGALLARPGGGYAVELVEGATHRRIVVRTGLFDDTA
                     GLVEVSGAGLAEGTKVEVPAP"
BASE COUNT          776 a         2328 c         2296 g          747 t
ORIGIN      
        1 gtcgggggcg acgcggtgct gcggcctccc gggccgcgcg cgcccggcgg cgtcgcctac
       61 gagagctggg cgttcgcgaa ccccgccggg gaccgggtgc acggcttcct ggccgccccg
      121 gccggcccgg gccctcaccc caccgtgctg ctcgtccacg gcggccccca ccaccacgac
      181 gccgacagct tcagccccga ggtccaggcg tgggtcgacc atggctgggc ggtggggctc
      241 gtcaactacc gcggctcgac cggctacggc aaggcctggc aggacgtgct cgagggcgac
      301 cccggccgcc ccgaggtcga ggacgtcgtc gccggccggg acgacctcgt cgcgcggggc
      361 gtcgccgacc cggaccgggt cgtgatcgcg ggcgcctcct ggggcgggta tgtcaccctg
      421 cagacgatcg gcaccgctcc cgacggatgg cgggccgccc tggccgtcgt gccggtcgcc
      481 gactacgtct ccgcgtacgc cgacgagtcc gaggggctgc aggccctcga ccggtccctg
      541 ttcgggggcg gcccggagga gaagccggag ctgttcgccg agcgctcgcc gatcacccac
      601 gccggcgagg tcaaggttcc cgtgctgctc atggtcggtg agaacgacac ccgctgcccg
      661 ctccagcagg tcctcaacta cgccgagcgc ctcaccgagc tgggcaagga gttcgagctg
      721 gaccgcttcg acgccggcca cggcgcgctc gtcgtcgacg agcgcatccg gcaggccgag
      781 gtggagctcg acttcgccgc ccgccacgtc cccggcgtcg cccagcccga agcgtaggcg
      841 actcactccc tggccggccc gggcgggacg gccacgacgg ccaggctccc ctggcccggg
      901 cgcaggacga gggtgaaccg gccgcccagc tcctcctcga ccagggcgcg gacgaggcgc
      961 aggccgaggc cgccgcccgt ccgcgggcgc ggctcggcca ccggccccgg gccgtcgtcg
     1021 gccacctcca gctcgatcgg cttgccgtcg cctccccgca gggtcaccgt caccgtgccc
     1081 ggcccctcgc cgaggccgtg ctcgatcgcg ttctggacga gttcggtcag cgcgacggcg
     1141 agcggggtgg cgatggccgc gtcggcgcgc accgacccgg cgacgagcgt gacgcgcacg
     1201 tcctcccgcc cgagcgagct ctcgagcatg cgcacgaccc ggcgggccac gtcggccatg
     1261 tcggcgccgc cctcgagctc ctcggtcagc gtctcgtgga cgagcgcgat cgacgcgatc
     1321 ctgcggaccg actcctcgag ggcggcgacc gccatcgggt ggtcgccgag ccgcctcgcc
     1381 tgcaggcgga gcagggaggc caccgtctgc aggttgttct tgacccggtg gtggacctcg
     1441 cgaatggtcg cctcccggcg ggagatcgtc cgttcgcgcc cgcgcagctc ggtcagctcc
     1501 cggcacaggg cgagggcgcc cctgcgggca cccgagccgt gcaggagcgg cacgacgcgg
     1561 acatgggtca cggccccgcc cagctccacg tcggcctcga ccgggcgacg gctcgacatc
     1621 gactcggcga ccagcacgcc gacacggccg ggcaccggct ggccgagcgg gagcgcgttg
     1681 gtgcccatgc ggcggaacgc cgaggtggcg ttcgggctcg cgaactcgac ccgaccgctc
     1741 gagtcgagca tgacgagccc gtcgccgacg cggggggacg agatcagctc gtgaccctcc
     1801 tggaacggga accagcccgt cgcgaccatc tcgagcaggg cgtcggccgc ccgcaggtag
     1861 gcggtctcga gcctgcccgt cggacggtcg ggctggtcgg cggcctcgac cgacatcacc
     1921 gcgacgacgt cgtcctcgtg gcggacgggc accgcctcca cccgcaccgg ctcggcgtcg
     1981 atgaacaccg gctcgggcga gcgcaggcgc cggccctccc tccaggcccg ctccacgtac
     2041 ggctgccagc ccggctcgac cacgtcgccg atcaggtcgg ccgggtagag cgttcgcgcg
     2101 gtgtacgggc ggagctgccc cacgatccgg aagctctcgc cgccctcgac gggcgcgaac
     2161 aggagcagat cggcgaagga caggtcggcg atgagctgcc aggcggccgt gaggccgcgc
     2221 aggtgctcga cggcggccgc gtccagcctg ggcccggccg tgcgggtgac ctcctgcatg
     2281 ccaccagggt agtcgcctcc tcgcctaccc gggccgcacg accaccacgc cgcgcatgcc
     2341 gtacgcgtag tggccgggca ggtggcaggc gtactcgagg cgcccggcgc cggtccagtt
     2401 cagggtgacc gtggtcgtcc gctccgtccc ggcgggcacc gagacctggc ccggggcgtc
     2461 gtggtgggcg cccgtcccct tctcgtgcag ctcctgcacg gcctcgtcgc ccaggatgaa
     2521 ctcgtggtcg atggggtcgg tgttgcggag cacgaaccgc accgtgctcc ccgcctcgac
     2581 ggcgacctcg gcaggctgga agtgcgagtg gtgcaccgtg atcgtgatga cgacgatccg
     2641 atcatgcgcg cggctgctgt gcgccgttcc gttcagccag agcgccgacc cggtgacgat
     2701 gccgaacagt gccgcggcga tgccggcgac ctgccatggg cgtggcatgc cccgcccttt
     2761 ccacgagaac gtgcggtcgc atggttggac gtgaccggcg ccgcgcgggt tcctattcgt
     2821 cgagcgcctc gtcgaggggg acgaggcgct gctggaggcc gaagatggcc gcctgggtgc
     2881 ggtcggcgag ccccagcttg gcgaggatgc tggagacgtg tgccttgacg gtctcctcgc
     2941 cgatcgtgag ggtccgtgcg atctggcggt tggagcggcc gcgggcgagg gcggccagga
     3001 cctcgagctc gcggggggag agctggtcga gcggccgccg gcggaccacg gcctcgcgga
     3061 cctcgcggac cagcctggcg gcgatcctgg gctcgagcac gctctcgccg cgggcggcgg
     3121 cccgcaccga ggtgacgact tggtcgactc cggcggtctt cggcaggtag gcgagcgccc
     3181 ctgcgtggac ggcggcgagc aggtgctcgt cgtcctggga cgaggtcagc atgagcacct
     3241 cggtggcggg ccggtcctgt ttgatctgcg ccagcacgcc gatgccgtcc acgccgggca
     3301 ggaacaggtc cagcagcacg acggtcggcg ccagccgcgc ggcgcgcagc gccgggtaca
     3361 gcccggcgcc gcgccgatcg ccagcgccgc cgtcaggccg ccccagatgg ccatggccgg
     3421 aacctggacc ggccagtggc gctggtgggc cacgccgaag gtgattgctg cgccgatcgc
     3481 catgccggcc aggccgccgg cgctggccag cagcagcgac tccaccagga actgcgcggc
     3541 gacgtgcccg cgggcggcgc cgagcgcgcg gcgcaggccg atctcgccgc ggcgctccag
     3601 caccccgatg accatgacgt tggcgatgcc gatcccgccc accagcagcg cgaccgcgcc
     3661 caggcccagg aacaggccgg tggtggcggc ctttgcggcc acgcgggcgg tcagcgcgtc
     3721 cgaggggcgg ctggcctgca cctgctcggg atgctctggg tcggcggtgg tggccagcag
     3781 gccggccacc gtggtggtct gctcggttcc ggtgcgcacg tagatgcggc tgggatggcc
     3841 ctggtagccc agcagccggg cggcggccgg gaagccgacc agcgccgagc ggtccagctc
     3901 cggcgccagc ggcaggggat ccaagatgcc gaccacggtg aaccagtggc cccccagcca
     3961 cacgcgggtg ggctggtcga gccgggcgat ccccagggtc gtggcggcct ggtagcccag
     4021 cacggtcgcc ggatacctcg cggtggccgg gttcaggaag gcgccgtggc gcacgcggcc
     4081 gtccagggcg gcgagcaggg ccgggtcgca ggcgcgtacc gccagcgcgc cggtctggcc
     4141 aggggggatc ttgtcgctgc ggtagacgtc gaggccctgc agctgggcgg tcggagcgac
     4201 ctggcggacc ccgtcgatgc ggccaagcgt cccgctggcc tgggtgggca gctcggcctc
     4261 ctggccctgg atggagcggc cgttgacgac ggtgagcagg ttggtgccga gccggtcgat
     4321 ctgggccagg aggtcggcct ggctggagcg ggtgatggcc agcaccgcga cgatcgcggc
     4381 gatgccgatg gccacgccaa gcgccgacag cccggcccgc agccgccggg cgcgcaggcc
     4441 gacggtgccg accggcagca ggtcggccgg ccgcagccgc gacctggggg tcgccgggga
     4501 tgtgctgcgc acgcgactca tgggcgccca ccacccgatc ccgagtcggc cacgatgcgc
     4561 ccgtcgcgca gctcgacccg gcgggtcatg gcggcggcga ccagggggtc gtgggtgacc
     4621 accaccaggg tgaccccctg ccggttgagc tgctgcagca acgccaccag ctcgccggtg
     4681 gcggccgagt cgaggttgcc ggtgggctcg tcggccaaca ccacctgcgg ctggccgacc
     4741 aacgcccggg cgatcgccac ccgctggcgc tccccacccg acagcgcggt ggggcggtgg
     4801 cccagccgat ggcccagccc gacccgctcc agcgccagcc gcgccagccg ccgccgctcg
     4861 gcgccgggca ggccacggta cagcaggccg gtggcgacgt tgtccagcgc gctcacgtgc
     4921 tccagcagga agaactgctg gaagaccacc cccaggcggt gggcgcgcag cccgcgagac
     4981 gccggtccga cagcgccgcc agcgcctggc cggccacccg caccaccccg ctggtgggcc
     5041 ggtccagcgc cgccatcagg tgcagcaggg tggtcttccc cgaccccgac ggccccacca
     5101 ccgcgaccag ctccccgcgg gtgacggtca gggtgacgcc gcgcaccggc gccaccggcg
     5161 ggctgcccgg atagaccttg ccgaccccct cgagctccag caccggcgtg gccgcctgcg
     5221 cgagcgcggc cggcgatggt ggtggggtgg cggtcatggc gccggcacct cgaccttcgt
     5281 gccctcggcg agcccggcgc ccgagacctc caccagcccg gcggtgtcgt cgaagagccc
     5341 cgtgcgcacc acgatccggc ggtgcgtggc gccctcgacc agctcgacgg cgtacccgcc
     5401 gccgggacgg gccagcagcg cgccgatcgg caccgccagc acccccttgt gcgcctgggt
     5461 ggtgatcgcc acctgcaccg gcgcctggtc caggtcggcc gccgcccgcg ggtcggccag
     5521 cccgaccgtc accgagacgg tcgcctgccc ggcaccgttg ccgttgcccc cttggctctg
     5581 gtcctggccg tcctggccgg tgccggtggc gggctgcacg gccacgcggc ccacggtggt
     5641 caccgtgccg gggatcgtcc ccccgtcggg cagcgtgacc aggactcggt cgccgcggcg
     5701 caccagctgc tgcacggccg ggtccaacgc gaccgtgacc agcggtcggg tcgaggtcgc
     5761 cgtcaggatc gccgtgcccg cccccagcgg cgcccccacc gtcgcgctga gggtggtcac
     5821 ccggatcggg ccgggcagga acattatctg ccccagcgcc accgcaccgg tctgggaccg
     5881 gccgatggcc agctgccagc gcttgaccgc cgccgccgtc gcccagctgt agtggcggtc
     5941 cacggtgatg gcgcggtcgg ggtcaaagcc caacgcgacc aggttggcct ccagctgccg
     6001 cacgtccggc ccgtcggtca tgccaagccc aaaggtccgc cacgccggcc tggtgccata
     6061 ccacaacggc accaggtggc cgtccacctc atacagcgcc tgcccccggc ccaccaccgt
     6121 gccggcggcg ggcagccgag tcaggat
//
LOCUS       DASPYY010000168         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2418265, whole genome shotgun sequence.
ACCESSION   DASPYY010000168 DASPYY010000000
VERSION     DASPYY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418265"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VEP73_02510"
     CDS             complement(<1..1290)
                     /locus_tag="VEP73_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT25342.1"
                     /translation="MPRLYVDGGWVAAASGAQREVQNPYDQSVIGKVDEAGPADVDAA
                     IRAARTAFDSGAWPGTAATQRGALLLRVADLLERDRDLVARRESLDTGKRLAESEYDV
                     DDVVRVFRYYGGVAAEGAGRIVDTGSPDVVSRVVYEPVGVCALITPWNYPLLQASWKV
                     APALAAGCTFVLKPSELTPSTAIHLIRLLAEAGLPAGVANLVLGGGPAVGAALTESPL
                     VDLVSFTGGLATGKRVMAAAAATVKKVALELGGKNPNIVFADADREAALDLALSGVFA
                     HSGQVCSSGARLVVQDVIHDEFVAELVVRADRIRLGGPFDPQTETGSLISAAHLAKIE
                     AYVAGALAEGATLACGGSVPDDPDLKNGFFYLPTILTGCRTEMRCVQDESFGPVLTVE
                     RFTDEDDAVRIANDTEYGLAGAVWTQDAGKAQRVARRL"
     gene            complement(1432..2181)
                     /locus_tag="VEP73_02515"
     CDS             complement(1432..2181)
                     /locus_tag="VEP73_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT25343.1"
                     /translation="MQSVDRAVAAMEILARRGAARVTEVAGELGIHKSTAFRLLSTLE
                     ARGLVEQDGDRGRYRLGFGVVRLAGAATGRLELNQYSRPICERLAEQAGETVNLAVHD
                     GDHAINVEQVMGSASVTSVNWVGQRTPLHATSSGKVFLAHLPAAELAALLARPLARFT
                     ANTIVDPPALSEQLDAIRADGFAYSVEELEIGLNAVAAPVRSFDGAVVAAVSVSGPAY
                     RITPGRIAEFGGLVVAAGNAISRRIGFQRAG"
BASE COUNT          295 a          788 c          801 g          301 t
ORIGIN      
        1 cagccgccgg gcgacccgct gggccttccc ggcgtcctgc gtccagacgg ctccggcgag
       61 tccgtactcg gtgtcgttgg ctatgcgtac ggcgtcgtcc tcgtcggtga agcgctcgac
      121 ggtcagcacc gggccgaacg actcgtcctg cacgcagcgc atctctgtcc ggcagccggt
      181 gaggatcgtc ggaaggtaga agaagccgtt cttgaggtcg gggtcgtccg gcaccgagcc
      241 gccgcacgcg agcgtggcac cctcggccag cgcaccggct acgtatgcct cgatcttggc
      301 cagatgcgcc gcggagatca gcgagccggt ctcggtctgc gggtcgaacg gcccgccgag
      361 ccggatccgg tctgcccgca cgaccagctc ggccacgaac tcgtcgtgga tcacgtcctg
      421 gacgaccagc cgtgcgccgg acgagcagac ctgacccgag tgcgcgaaca ctccgctgag
      481 cgcgaggtcg agcgcggcct cgcggtcggc gtccgcgaac acgatgttcg ggttcttgcc
      541 gcccagctcg agcgccacct tcttgacggt cgcggcggcg gcggccatca cccgcttgcc
      601 ggtcgccagc ccgccggtga acgagaccag gtcgacaagc gggctctcgg tgagcgcggc
      661 gccgaccgcg gggccgccac cgagcacgag gtttgcgacg cccgccggta gcccggcctc
      721 ggcgagcagc cggatcaggt ggatcgcggt cgacggggtg agctcgctcg gcttgaggac
      781 gaacgtgcag ccggccgcca gggcgggcgc gaccttccag gaggcctgca gcagcgggta
      841 gttccagggg gtgatgagcg cgcagacccc taccggctcg tagaccactc ggctgacgac
      901 gtcggggctg ccggtgtcga cgatccggcc ggcgccctct gcggcgaccc cgccgtagta
      961 ccggaagacc cggaccacgt cgtcgacgtc gtactcgctc tcggccagcc gcttgccggt
     1021 gtccagagac tcccggcgcg cgaccaggtc gcggtcgcgc tcgaggaggt cggcgacgcg
     1081 caggagcagt gcgccgcgct gggttgcggc cgtcccgggc caggcccctg agtcgaaggc
     1141 ggtacgagcc gctcggatgg ccgcgtcgac gtcggccggc ccggcctcgt cgaccttgcc
     1201 gatgacgctc tggtcgtagg ggttctggac ctcgcgctgc gcacccgagg ccgcggccac
     1261 ccagcccccg tcgacgtaga gtctcggcat gggtacctcg ttccgtcgag gtagcgctcg
     1321 aggtagcgtt gcgtattacg aaaaccctgt gcgtcggtgc aacagcctga tcgctccccc
     1381 ggcgggcgtc aaggctgtgc gggacggcgc cgcgtccggc cggagctgcc gtcagccggc
     1441 ccgctggaac ccgatccgcc gcgagatggc gttcccggcg gccaccacca gcccgccgaa
     1501 ctcggcgatg cggccgggcg tgatccggta ggcgggaccg gacacgctca cggccgcgac
     1561 cacggcgccg tcgaacgagc ggaccggcgc ggcgaccgcg ttgagcccga tctcgagctc
     1621 ctcgacgctg taggcgaacc cgtcggcccg gatggcgtcg agctgctccg agagcgccgg
     1681 cgggtcgacg atcgtgttcg cggtgaagcg cgccaggggc cgggccagga gcgccgccag
     1741 ctccgccgcg ggcaggtggg cgaggaacac cttcccgctc gaggtcgcgt gcaggggggt
     1801 gcgctggccg acccagttca cgctcgtcac cgaggccgag cccatcacct gctcgacgtt
     1861 gatggcatgg tcgccgtcgt ggacggcgag gttgacggtc tcccccgcct gctccgccag
     1921 ccgctcgcag atcggccggc tgtactggtt cagctcgagg cgcccggtcg cggcgccggc
     1981 gagcctgacc acgccgaagc cgaggcggta cctgccccgg tcgccgtcct gctcgaccag
     2041 gccacgggcc tcgagcgtgg acagcaggcg gaacgccgtg gacttgtgga tgccgagctc
     2101 gccggcgacc tcggtgaccc tggccgcgcc gcggcgggcg aggatctcca tcgccgcgac
     2161 ggcgcggtcg accgactgca cgaga
//
LOCUS       DASPYY010000169         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_592374, whole genome shotgun sequence.
ACCESSION   DASPYY010000169 DASPYY010000000
VERSION     DASPYY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592374"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VEP73_02520"
     CDS             complement(<1..254)
                     /locus_tag="VEP73_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT25344.1"
                     /translation="MDATATDPAELVEQFAAGYPFELDDFQRRGCLALASGSGTLVAA
                     PTGAGKTVVGEFAVHLALSEGRKCFYTTPIKALSNQKYADL"
     gene            complement(322..1263)
                     /locus_tag="VEP73_02525"
     CDS             complement(322..1263)
                     /locus_tag="VEP73_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYT25345.1"
                     /translation="MRALVVCNPAARGGRGLRALPTVLERLRALEVEADVHRTTSLAD
                     ARAAARGAAATVDAVAAMGGDGVVGACAAGLAQAGPGARAALGVIPTGSGNDAARMLG
                     VPPGDPAAAAALLPTLARRRADLARVDGRHYLNVAGAGLDAEVNRLANDRLAWVPGRA
                     RYVGALLAELAVRQPSTFTLTLDGERHEVRAWFVAMANGPSYGGGMRIAPDARIDDGL
                     LDVVVVGAIHRPGFLATFPKVRSGRHVGHPAVSVHRACRVELAADRPLLVYADGERHG
                     ELPVVFEACPGAIHVLAAADAGGSAAADAGGSGGPPR"
     gene            complement(1277..2059)
                     /locus_tag="VEP73_02530"
     CDS             complement(1277..2059)
                     /locus_tag="VEP73_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25346.1"
                     /translation="MAIKGKTKRSQGRPVRRPAAGPRIQAVERREKWYRATAFPVTLA
                     VIALLGTLIAAANRTQEGFARDDVRRFTDAVRTPLADTTDVIGAGTTARPGFASAADL
                     AAGKVRPADLAKRAKDWQTRLSDVRARVANTSLGTQPQGAGLDGNPANETGGHVPMLS
                     SVRDAYTAAVGTYFEAAHMYELAGSAPAKSQLAGQLVSEGNATAKRGEEAMDAAASML
                     ARLNARYHLDVKRQMPGESAGSYAQRYGGAPAPTGGSSPLGN"
BASE COUNT          222 a          809 c          794 g          260 t
ORIGIN      
        1 aggtcggcgt acttctggtt ggacagcgcc ttgatcgggg tggtgtagaa gcacttgcgg
       61 ccctcggaca acgccaggtg caccgcgaac tcgcccacca cggtcttgcc ggcgccggtg
      121 ggcgcggcga cgagggtgcc cgaccccgag gcgagcgcga ggcagccccg gcgctggaag
      181 tcgtcgagct cgaacgggta gccggccgcg aactgctcga cgagctcggc ggggtcggtg
      241 gctgtggcgt ccatcaccct gtcatgctgc caccggtcgg cgccggccgc cgtgcgccgt
      301 ccccaccggc cgcccggcgg ctcagcgggg tggcccgccg gaccccccgg cgtccgcggc
      361 ggcggacccc ccggcgtccg cggcggcgag cacgtggatc gcgccggggc atgcctcgaa
      421 gaccaccggc agctccccat ggcgctcccc gtccgcgtac accaggaggg gccggtccgc
      481 ggccagctcg accctgcacg cccggtgcac cgacacggcc gggtgaccga cgtgccggcc
      541 cgaccgcacc ttcgggaagg tggccaggaa cccgggccgg tggatggcgc cgaccaccac
      601 cacgtccagc aggccgtcgt cgatccgggc gtccggcgcg atccgcatgc cgcccccgta
      661 gctcggcccg ttcgccatcg ccacgaacca ggcccgcacc tcgtgccgct cgccgtccag
      721 ggtgagcgtg aaggtggaag gctggcgcac ggcgagctcg gcgagcagcg cgccgacgta
      781 gcgggcccgg ccagggaccc aggcgagccg gtcgttggcg agccggttca cctcggcgtc
      841 caggccggcc cccgcgacgt tgaggtagtg gcggccgtcg accctggcga ggtcggcgcg
      901 ccgcctggcc agggtcggca gcagggcggc ggcggccgcg gggtcgccgg gcgggacgcc
      961 gagcatcctc gccgcgtcgt tgccgctccc ggtcgggatc acgccgaggg cggcgcgggc
     1021 gcccgggccg gcctgggcga ggccggccgc gcacgccccg acgacgccgt cgccgcccat
     1081 cgcggcgacg gcgtccaccg tcgcggccgc tccgcgcgcg gccgcacggg cgtcggcgag
     1141 cgaggtcgtg cggtgcacgt ccgcctcgac ctcgagcgcc cgcaggcgct cgagcaccgt
     1201 ggggagcgcg cggagccccc gcccgccccg ggcggccggg ttgcagacca ccagggcgcg
     1261 catcgcgcgg gccggcctag ttgccgagcg gcgagctgcc gccggtcggc gccggggcgc
     1321 cgccgtagcg ctgggcgtag gacccggccg actcgccggg catctgccgt ttcacgtcga
     1381 ggtggtagcg ggcgttcagc cgcgcgagca tcgaggcggc cgcgtccatg gcctcctcgc
     1441 cccgcttggc cgtggcgtta ccctcgctca cgagctgccc ggcgagctgg ctcttggccg
     1501 gcgcggaccc ggcgagctcg tacatgtgcg ccgcctcgaa gtacgtgccg accgcggccg
     1561 tgtaggcgtc gcggacgctc gacagcatcg gcacgtgccc gccggtctcg ttggccgggt
     1621 tgccgtccag cccggcgccc tgcggctgcg tgccgaggct cgtgttcgcg accctggcgc
     1681 ggacgtcgga gagccgggtc tgccagtcct tcgcccgctt ggccaggtcg gccggcctca
     1741 ccttgccggc ggccaggtcc gcggccgagg cgaaccccgg cctcgcggtc gtcccggcgc
     1801 cgatcacgtc cgtcgtgtcg gcgagcggcg tgcgcaccgc gtcggtgaac cggcgcacgt
     1861 cgtcgcgggc gaagccctcc tgggtccggt tggccgccgc gatcagcgtg ccgagcagcg
     1921 cgatcaccgc cagggtgacc gggaaggctg tggcccggta ccacttctcg cgccgctcga
     1981 cggcctggat ccggggcccg gccgccggcc gccggaccgg acggccctgg ctccgcttgg
     2041 tctttccctt gattgccacg ctccgttagc tcccttgcaa cgcct
//
LOCUS       DASPYY010000170         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1996293, whole genome shotgun sequence.
ACCESSION   DASPYY010000170 DASPYY010000000
VERSION     DASPYY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996293"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VEP73_02535"
     CDS             complement(<1..429)
                     /locus_tag="VEP73_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25347.1"
                     /translation="MSIVPAARRRVVMALFFFPRGGSVYVARHLARLLPAAGWDVTLV
                     AGSLGTAGGRSHAPTFFQGAEVYTVDYTAAAAVRDPLDAVVPFQPSYEDRPGAPDRVF
                     ARTGDDAYERLVAAWEKALGEAGAAGADVLHLHHLTPANEA"
     gene            554..1339
                     /locus_tag="VEP73_02540"
     CDS             554..1339
                     /locus_tag="VEP73_02540"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYT25348.1"
                     /translation="MTEPLFTGVGVALVTLFDPMGEVDAKATADHAAQLADLGMRAVV
                     VAGSTGEAAGLTLDERIALLTEVRRAVPAGVPVVAGTGAPATRQAVALTKAAVEHGAD
                     AVLVLSPPRVSGPRAYYGAVADAAGGLPMLAYHFPAVSAPGVPLDVLPDLPVQGVKDS
                     SGDPERLLAELRGFQGWVYVGSSALLAYAGPLGCQGAILALANAMPELCAAAFSGSAA
                     AQLELLPGHLAARRDFPNGLKELVAGRFGTSRTARLGWGGGRP"
     gene            1336..1971
                     /locus_tag="VEP73_02545"
     CDS             1336..1971
                     /locus_tag="VEP73_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYT25349.1"
                     /translation="MTRLLAFLAVSVVVICTPGPDTALTIRNTLAGGRRGGVWTAAGV
                     AIGQSVWTLAASAGVAGLLRASEPAFLAVKVAGAAYLVVLGVQSLHAAWSRRGHGDAP
                     ARRASERRACDPSLALRQGILSNLGNPKMAAFFTGLLPQFAPGGRPSFLALSLLGLVF
                     CLLTLAWLAAYSFAVAKARVLLHRPRVRRALETLTGCVLVALGVRVAAEHR"
     gene            2167..2340
                     /locus_tag="VEP73_02550"
     CDS             2167..2340
                     /locus_tag="VEP73_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25350.1"
                     /translation="MKYMILLYGSQQDYDGLAGKATDKPAWSAEDVAAMSSSWSRSPR
                     SWWSPGSSLTREV"
     gene            2304..2582
                     /locus_tag="VEP73_02555"
     CDS             2304..2582
                     /locus_tag="VEP73_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYT25351.1"
                     /translation="MESRELVDTRGLTAPVHTRRIQLQSGVPTVTDGPYPETQEVLAG
                     FWIVECESFDRATEIAARLTTCPGPGDAYEDNVVDVRPIADALAELDV"
     gene            2591..>3511
                     /locus_tag="VEP73_02560"
     CDS             2591..>3511
                     /locus_tag="VEP73_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT25352.1"
                     /translation="MPDTEVEDLLRRLAPQVLGAVVRRYGHFDAAEDAAQEALLAAAM
                     QWPEDGIPDNPRGWLITVASRRLTDLLRSEQARRRREDTVARWMLPDHWLAPAADRPA
                     SESDDTLILLSMCCHPSLSPASQIALTLRAIGGLTTAEIARAFLVPEATMTRRISRAK
                     QRIKDSGIPFGMPPGPERTERLGAVLHVLYLIFNEGYASTSGPSLQRSELAAEAIRLA
                     RMVHRLLPDDGEVTGLLALMLLTDARRPARTGPDGGLIPMAEQDRSLWNAGYIAEGVA
                     LITDALSRGATGPYQLQAAIAAIHDEAPSAE"
BASE COUNT          440 a         1301 c         1269 g          501 t
ORIGIN      
        1 cgcctcgttc gccggggtga ggtggtgcag gtggagcacg tcggcgccgg ccgcgccggc
       61 ctccccgagc gccttctccc aggccgcgac cagccgctcg taggcgtcgt cgccggtgcg
      121 ggcgaagacg cggtccggcg cgcccgggcg gtcctcgtaa gacggctgga acggcaccac
      181 ggcgtcgagc gggtcccgga cggcagcggc ggcggtgtag tcgaccgtgt agacctcggc
      241 gccctggaag aaggtcggcg cgtgggacct cccgccggcc gtgccgagcg agccggccac
      301 cagcgtcacg tcccagccgg cggcgggcag cagccgggcg agatgcctgg cgacgtagac
      361 ggagccgccc cgcgggaaga agaacagcgc catgaccact ctgcggcgcg cggcggggac
      421 gatgctcatc gcgggaacct acccaggccg gacctcgagc gcaacgtcgc cgccggagcg
      481 ccggagcgcc ggagcgccgg agcgccgggg cgccggggta tcctcgagcc gcgccctctt
      541 cctggaggtc ttcatgacgg agccgctgtt caccggcgtc ggcgtcgccc tcgtcaccct
      601 gttcgacccc atgggcgagg tcgatgccaa ggccaccgcc gaccacgccg cccagctcgc
      661 cgatctcggc atgcgcgcgg tcgtggtcgc cggctcgacc ggggaggcgg ccgggctcac
      721 cctcgacgag cgcatcgcgc tgctcaccga ggtccgccgg gcggtcccgg ccggcgtccc
      781 ggtcgttgcg ggcaccggcg cccccgccac ccgccaggcc gtcgcgctca ccaaggccgc
      841 cgtcgagcac ggcgccgacg cggtgctcgt cctctccccg ccgcgggtct ccggcccgcg
      901 ggcgtactac ggcgcggtgg cggacgcggc cggcggcctc ccgatgctcg cctaccactt
      961 cccggccgtg tcggcgccag gcgtccccct cgacgtgctc cccgacctcc ccgtccaggg
     1021 cgtcaaggac tcctcgggcg accccgagcg cctgctcgcc gagctgcgcg gcttccaggg
     1081 ctgggtctac gtcggctcgt ccgccctgct cgcctacgcc gggccgctcg gctgccaggg
     1141 ggccatcctc gcgctcgcca acgctatgcc ggagctgtgc gcggccgcgt tctcggggag
     1201 cgccgccgcc cagctcgagt tgctccccgg ccacctcgcg gcccgccgcg acttcccgaa
     1261 cgggctcaag gagctcgtcg ccgggcggtt cggcacctcc cggaccgccc ggctcggctg
     1321 gggcggcggc cggccgtgac caggctgctg gcgttcctgg cggtctcggt ggtggtgatc
     1381 tgcacccccg ggcctgacac cgcgctgacg atccgcaaca cgctcgccgg cgggcggcgc
     1441 ggcggggtgt ggacggcggc cggcgtcgcc atcgggcaga gcgtctggac gctcgcggcg
     1501 agcgcgggcg tcgccggtct cctgcgcgcg tccgagcccg cgttcctcgc cgtcaaggtg
     1561 gccggtgccg cctacctcgt cgtgctcggc gtgcagtccc tgcacgcggc ctggtcgcgc
     1621 cgcgggcacg gcgacgcgcc cgcgaggcgt gcctccgagc ggcgtgcctg cgacccctcg
     1681 ctcgcgctgc gccaggggat cctcagcaac ctgggcaacc ccaagatggc agccttcttc
     1741 accggcctgc tcccgcagtt cgccccaggc ggacggccgt cgttcctggc gctgtcgctg
     1801 ctcggcctgg tgttctgcct gctcacgctc gcctggctcg cggcgtacag cttcgcggtc
     1861 gcgaaggcac gcgtgctcct ccaccgcccg cgggtgcgcc gcgcgctcga gacgctcacc
     1921 gggtgcgtgc tcgtcgccct cggcgtccgc gtcgcggccg agcaccgatg acgcgctcct
     1981 ggcggctctc gacccggatg cccgccgccg tgccgcgctg gtgcaggcgg tcgtggccat
     2041 gcccgacgat gaccgcgtcg aggcaatgat tcgcggctcg gtggggggtg gatgtccgag
     2101 gtgggccggc ggccccgacg tctctggtga gaggcactgg agaggtgccg gcagagagga
     2161 gagatcgtga agtacatgat cttgctctac ggctcgcagc aggactacga cggtctggcc
     2221 gggaaggcca ccgacaagcc ggcctggtcc gcggaggacg tcgcggcgat gtcctcttca
     2281 tggagtcgtt caccaaggag ctggtggagt ccagggagct cgttgacacg cgaggtctga
     2341 ccgctccggt ccataccagg agaatccagt tgcaaagcgg tgttccgacc gtgacggacg
     2401 ggccgtatcc cgaaacccag gaggtcctcg ccggcttctg gatcgtggag tgtgaaagct
     2461 tcgaccgggc gaccgagatc gccgcccggc tgacgacctg cccgggtccg ggcgatgcct
     2521 acgaggacaa cgtggtggac gtacggccga tcgccgatgc cctggccgag ctggacgtct
     2581 gatcgctccc gtgcctgaca ccgaggtcga ggacctgctg cgccggctgg cgccgcaggt
     2641 cctcggcgcg gtggtacggc gttacgggca tttcgacgcc gccgaggacg cggcgcaaga
     2701 ggcgctcctc gcggccgcca tgcagtggcc ggaggatggc atcccggaca atccgcgagg
     2761 ttggctgatc acggtcgcct cgcgcaggct gaccgacctg ctgcgcagcg agcaggctcg
     2821 ccggcgccgt gaagacaccg tcgcccggtg gatgctgccc gaccactggc tggcgcccgc
     2881 cgccgacagg ccggcatcgg agtctgacga cacgctcatc ctgctgtcca tgtgttgtca
     2941 cccctcgctg tcgccggcat cgcagattgc gctcaccttg cgggcgatcg gcggcctgac
     3001 caccgcggag atcgctcgtg cgtttctcgt gccagaggcg accatgaccc gacgcatcag
     3061 ccgggccaag cagcgcatca aggacagcgg tattcccttc ggcatgcccc cgggcccgga
     3121 gcgcaccgaa cggctcggcg cggttctcca cgtgctctac ctgatcttca acgagggcta
     3181 cgccagtacg tcaggtccga gcctgcagcg cagtgagctg gcggccgaag cgatccggct
     3241 ggcccgcatg gtccaccggc tgctgcccga tgatggcgag gtgacggggc tgcttgcgct
     3301 catgttgctc accgacgcgc gccggccggc gcgtaccggg ccggatggcg ggttgatccc
     3361 gatggccgag caggatcgaa gcctctggaa cgccggctac atcgctgagg gcgtggcgct
     3421 catcaccgat gcgctctccc ggggagcaac cggcccgtac cagctgcagg cggcgatcgc
     3481 cgcgatccac gacgaagcgc caagcgccga g
//
LOCUS       DASPYY010000171         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_311123, whole genome shotgun sequence.
ACCESSION   DASPYY010000171 DASPYY010000000
VERSION     DASPYY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311123"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /gene="iolD"
                     /locus_tag="VEP73_02565"
     CDS             <1..1882
                     /gene="iolD"
                     /locus_tag="VEP73_02565"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HYT25353.1"
                     /translation="LIPAALGIFGHGNVAGLGQALDQLSDALPFIQGRNEQALVHVAT
                     AYAKASRRHATLAVTASIGPGALNMVTGAGVATVNRLPVLLLPGDSYATRRQGPVLQQ
                     LQHPIEADTSVNDAFRPLARFFDRITRPEQLLTALPQAMRVLTDPVDTGAVVLALPQD
                     IQPHAYDYPAELFAEREWTIRRPPADPDEVEAVTRLLAAAERPLIIAGGGVVYAGATR
                     ELEALAASAGIPVAETFAGKGAVQQRAWWQLGGIGLEGTPATNDLAREADLVLTVGSR
                     LTDFATASQSLFQHPQVRFASINVNPYDAGRLGATGIVADARRALAALAEAVRRAGTS
                     SPAGWQERVRTRNRRWQAERAAAIDPDTPFDLSALEPDSDVMSDTGAVLTQGQIIGLL
                     QEHARAGDVIIAAAGGPPGDLQKVWDATEGRFTHLEFGFSCMGYELPASIGVRLAEPD
                     PARRIVSFLGDGTFLMAPTELVTAAQEGLPITVVIPENRGYQVIHRLQMLRSGREFGN
                     EFRYRDGSLDLTGAGAGAAAKPPRLEGEYLDVDLVKVAEGLGARALRATTADEVRAAL
                     ATTRDHSGPVVIVVPAIPHADLPGADVWWDVAPAEVAEQEAVARLRAEYEDGLAAQRW
                     HG"
     gene            1879..2709
                     /locus_tag="VEP73_02570"
     CDS             1879..2709
                     /locus_tag="VEP73_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYT25354.1"
                     /translation="MSTADTTVPAGPSPLDRGRLRARLAAGQATLGTFVGAASPITAE
                     ACAAAGVDWLVLDLEHGSGGEEQVRDLVPAAGAYGVPTVVRVESAARIRIGRVLDLGA
                     AGVMLPRMDTAAEVAQAVRHLRYPPAGDRGVATYNRACRFALDPGALARAAGEVLGVV
                     QIESASAVDQVEEIAAIDGVDVLFVGPQDLSHNLGVPFQLQSPTYRAAVDRVRRAAER
                     HGKTCGLLVGDGAAAATRHTEGWRFVAIGCDTSLLAAAVAAELGRARPSTRDIRGGHP
                     "
     gene            2706..>3807
                     /locus_tag="VEP73_02575"
     CDS             2706..>3807
                     /locus_tag="VEP73_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT25355.1"
                     /translation="MTSTTTNNNTSSATSDIGVGLISVGWMGKLHSRAYQAVPYVYPE
                     AGIRPRLVHAVDTAPDRVVYAREVLGYERGSSDYREVLADPEVEVVSICAPNALHREI
                     GVAAAEAGKHFWIEKPVGRTVKETAAVAAAARAAGVVTSIGYNYRHAPAVEHSRELIA
                     SGALGRVTNLRAVFFSGYASAPKGALSWRFKRELAGSGALGDLLSHVVDLMQYVVGPV
                     VEVTALTSTTYTERPILPMGSATHFAVIEDSELGLVENEDYAATLVRFADDARGAGAV
                     GTLEASRTIVGPQCGLGFEIYGTDGSLVWNFEQMNELRLCLGRSGPHQGYTTVLGNPH
                     LGDYGRFQPGPGIAMGYDDLKVIEAKKFLVAVA"
BASE COUNT          521 a         1500 c         1310 g          476 t
ORIGIN      
        1 gctcatcccg gccgcgctcg ggatcttcgg ccacggcaac gtggccggcc tcgggcaggc
       61 gctagaccag ctcagcgacg ccctcccctt catccagggc cgcaacgagc aggccctcgt
      121 ccacgtcgcc accgcctacg ccaaggcatc ccggcggcac gccaccctgg ccgtcaccgc
      181 ctccatcggc ccgggcgcac tcaacatggt caccggcgcc ggggtggcca ccgtcaaccg
      241 cctgccggtc ctgctgctgc ccggcgactc ctacgccacc cgccgccagg gcccggtcct
      301 gcagcagctc cagcacccga tcgaggccga cacctccgtc aacgacgcct tccggcccct
      361 ggcccgcttc ttcgaccgca tcacccggcc cgagcagctc ctgaccgccc tgccgcaggc
      421 catgcgggtg ctcaccgacc cggtcgacac cggcgccgtc gtcctcgccc tcccccagga
      481 catccagccg cacgcctacg actacccggc cgagctgttc gccgagcggg aatggacgat
      541 ccgccgccca cctgctgacc ccgacgaggt tgaggcggtg acgcggctgc tcgccgccgc
      601 cgaacggccg ctgatcatcg ccggcggcgg ggtcgtctac gccggcgcca cccgggagct
      661 ggaggcgctg gccgcctcgg ccgggatccc ggtggccgag accttcgccg gcaagggcgc
      721 ggtccagcaa cgcgcctggt ggcagctggg cggcatcggc ctggagggca ccccggccac
      781 caacgacctg gcccgcgagg ccgacctggt cctgaccgtc ggatcgcgcc tcaccgactt
      841 cgccaccgcc tcccagtcgc tgttccagca cccccaggtc cgcttcgcca gcatcaacgt
      901 caacccgtat gacgcggggc ggctcggcgc caccgggatc gtggccgacg caagacgcgc
      961 cctggccgcg ctggccgagg ccgtccggcg ggccggcacc agcagccccg ccggctggca
     1021 ggagcgggtc cggacccgca accgccggtg gcaggccgag cgggccgccg ccatcgaccc
     1081 cgacacgccc ttcgacctct cggccctgga gcccgactcc gacgtcatga gcgacaccgg
     1141 ggccgtgctc acccagggcc agatcatcgg gctgctgcag gagcacgcca gggccggcga
     1201 cgtcatcatc gccgccgccg gcggcccgcc cggagacctg cagaaggtgt gggacgccac
     1261 cgagggccgc ttcacccacc tggagttcgg gttctcctgc atgggctatg agctgccggc
     1321 ctccatcggg gtgcgcctgg ccgaacccga ccccgccagg cggatcgtct cgttcctggg
     1381 cgacggcacc ttcctgatgg ccccgaccga gctggtcacc gcggcccagg agggcctccc
     1441 gatcaccgtc gtgatcccgg agaaccgtgg ctaccaggtc atccaccgcc tgcagatgct
     1501 gcgcagcggc cgcgagttcg gcaacgagtt ccgctaccgc gacggcagcc tcgacctgac
     1561 cggcgccggt gcgggcgcgg ccgccaagcc gccacgcctg gaaggcgagt acctagacgt
     1621 ggacctggtc aaggtggccg agggcctcgg cgcccgcgcc ctccgagcga ccaccgccga
     1681 cgaggtccgg gcggccctgg ccaccacccg cgaccacagc ggtccggtgg tgatcgtggt
     1741 ccccgccatc ccccacgccg acctgcccgg cgccgacgtg tggtgggacg tcgcccccgc
     1801 cgaggtcgcc gagcaggagg cggtcgcccg gctccgggcc gaatacgagg acgggctggc
     1861 cgcccagcgg tggcacggat gagcaccgcc gacaccaccg tcccggccgg gccgtcgccg
     1921 ctggaccggg gccggctacg ggcccggctg gccgccggcc aggccaccct gggcaccttc
     1981 gtcggcgccg cctcgccgat caccgcggag gcgtgcgccg ccgccggggt cgactggctg
     2041 gtcctggacc tggagcacgg gtcgggcggc gaggagcagg tccgcgactt ggtcccggcg
     2101 gccggcgcct acggcgtccc gaccgtagtc cgggtcgagt cggccgcccg catccgcatc
     2161 ggccgggtcc tggacctggg cgcggccggg gtcatgctgc cccggatgga cacagccgcc
     2221 gaggtcgccc aggccgtccg gcacctgcgc tacccgcccg ccggtgaccg gggggtggcc
     2281 acctacaacc gggcctgccg cttcgccctc gacccgggcg ccctggcccg ggccgccggc
     2341 gaggtcctgg gcgtggtcca gatcgagtcg gcgtccgccg tcgaccaggt cgaggagatc
     2401 gccgccatcg acggggtcga cgtgctgttc gtgggccccc aggacctcag ccacaacctc
     2461 ggcgtgccgt tccagcttca gtcccccacc taccgggcgg cggtcgaccg ggtccgccgg
     2521 gccgccgagc ggcacggcaa gacctgcggg ctgctggtcg gcgacggcgc ggccgcggcc
     2581 acccggcaca ccgaaggctg gcggttcgtg gccatcggct gcgacacgag cctgctggcc
     2641 gccgccgtcg ccgccgaact cgggcgagca cgaccatcca cccgtgacat acggggaggt
     2701 catccatgac cagcaccacg accaacaaca acaccagcag cgccaccagc gacatcggcg
     2761 tcggcctgat cagcgtcggc tggatgggca agctgcacag ccgcgcctac caggctgtgc
     2821 cctacgtgta tcccgaggcc gggatccgtc cccggctggt gcacgcggtc gacaccgccc
     2881 ccgaccgggt ggtctacgcg cgggaggttt tgggctacga acggggcagc agcgactacc
     2941 gcgaggtcct ggccgacccc gaggtggagg tcgtctccat ctgcgccccc aatgccctgc
     3001 accgcgagat cggcgtggcc gccgccgagg ccggcaagca cttctggatc gagaagccag
     3061 tcgggcggac ggtgaaggag acggcggcgg tcgccgccgc cgcccgcgcc gccggcgtgg
     3121 tcacctccat cggctacaac taccggcacg ccccggcggt cgagcacagc cgggagctga
     3181 tcgcctccgg cgccctgggc cgggtcacca acctccgcgc ggtgttcttc tctgggtatg
     3241 cctccgcgcc caagggggcg ctgtcgtggc ggttcaagcg ggagctggcc ggcagcggcg
     3301 ccctcggcga cctgctcagc cacgtggtcg acctgatgca gtacgtcgtc ggccccgtgg
     3361 tcgaggtcac ggccctgacc agcaccacct acacggagcg gccgatcctg ccgatgggct
     3421 cggccaccca cttcgccgtc atcgaggaca gcgagctggg cctggtggag aacgaggact
     3481 acgcggccac cctggtccgg ttcgccgacg acgcccgggg agcgggtgcg gtgggcaccc
     3541 tggaggcgtc gcgaaccatc gtcggccccc agtgcggcct cgggttcgag atctacggca
     3601 ccgacggctc gctggtttgg aacttcgagc agatgaacga gctgcggctc tgcctgggtc
     3661 gcagcggccc ccaccagggc tacaccaccg tgctcggcaa ccctcatctc ggcgactacg
     3721 gccggttcca gcccggccca gggatcgcca tgggctatga cgacctcaag gtgatcgagg
     3781 ccaagaagtt cctggtggcc gtggccg
//
LOCUS       DASPYY010000172         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_30517, whole genome shotgun sequence.
ACCESSION   DASPYY010000172 DASPYY010000000
VERSION     DASPYY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30517"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VEP73_02580"
     CDS             complement(<1..627)
                     /locus_tag="VEP73_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HYT25356.1"
                     /translation="MCGIIAVVRRPSDRTPPAADDVVERMERAWAAASQAQRVDGTAA
                     GHLDRAAAELEALDGLLRGSAGLACLLGDRAGRAALDRRAGELEHLVDALEAGLDAGR
                     HGEPRQVTIEAVNAALVRLKDALWAVRRDRLRTAEAVEALAGPAPRRTTVEAFASIQT
                     ALSALDRLEVRGRDSAGLHVLVTGHGLDLADPEVAPMLAARRQDRLFTS"
     gene            894..2396
                     /gene="rfaE2"
                     /locus_tag="VEP73_02585"
     CDS             894..2396
                     /gene="rfaE2"
                     /locus_tag="VEP73_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HYT25357.1"
                     /translation="MSETLASVVDRFDRLSVLVLGESILDVYLRGSSTRLCREAPVPV
                     VDVAGRAEAPGGAANAAVNARALGARVTFLSVVGDDREGAAVLALLAEAGVDARRVLV
                     RPGRRTLAKHRVVADGQVLVRFDEGGGGATDEGSDAELAELLAAEAAGHDAVLVSDYG
                     YGVLGAAVAGVLRRLQAEAPRVLVVDARHPTAYRGVGATAVKPNYDEAVQLLGGGRFD
                     PRGSRADQLERQGRRILSLLGAEVAAVTLDTEGAVLFERDGPPYRTYARPAASARAAG
                     AGDTFSSALTLALAAGAGVPAAGELASAAAAVVVAADGTAVCPRAALEEAVGGDRRIV
                     TAAELAARASLYRRQGRRIVFTNGCFDILHRGHITYLNRAKTLGDVLVVGVNADAGVR
                     RLKGPDRPLNALDDRMAVLAALSCIDHLVAFEDDTPAELIRAVRPDVFVKGGDYRRDT
                     VPEGPLVESLGGAVEILPFLEARSTTGIVERIRAGGPPRAGRRGPRERSA"
     gene            2393..>3045
                     /locus_tag="VEP73_02590"
     CDS             2393..>3045
                     /locus_tag="VEP73_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYT25358.1"
                     /translation="MSAAGALGASAAGARLVPGVARLAVLRANAVGDFLLALPALEAL
                     RRAYPDAEVVLLGSDWHRAFLAGRPSPVDRCVAVPPSRGVRDDRPPAPPEALDAFFAR
                     MRAERFDLALQLHGGGASSNPFVARLGAAATAGARAADAPGLDRTTAYNLYQPEVLRL
                     LEVVSLVGAAPVTLEPRVAVTAADRAESRAALPGDGRPLVAIHPGARDPRRRWPPER"
BASE COUNT          308 a         1169 c         1204 g          364 t
ORIGIN      
        1 ggaggtgaac agccggtcct gccgccgcgc cgcgagcatc ggggcgacct cggggtcggc
       61 caggtcgagc ccgtgcccgg tgaccagcac gtgcaggccg gcagagtccc gcccgcgcac
      121 ctcgaggcgg tcgagcgcgg acagcgccgt ctggatcgag gcgaacgcct cgaccgtcgt
      181 ccggcgcggg gccggcccgg cgagcgcctc gaccgcctcg gcggtccgga ggcggtcgcg
      241 gcggaccgcc cacagcgcgt ccttgagccg gaccagcgcg gcgttgaccg cctcgatcgt
      301 gacctggcga ggctcgccgt gccgcccggc gtcgaggccg gcctcgagcg cgtccaccag
      361 gtgctcgagc tcgcccgccc gccggtccag cgctgcgcgc ccggcccggt cgccgagcag
      421 gcacgcgagg ccggccgagc cgcgcagcag gccgtcgagc gcctccagct cggcggcggc
      481 ccggtcgagg tgcccggctg ccgtgccgtc gacgcgctgc gcctgcgagg ccgccgccca
      541 cgcccgttcc atgcgctcga cgacgtcgtc ggcggccggc ggggtgcggt cggacgggcg
      601 gcgcacgacc gcgatgattc cgcacatggt tgctccctta cagcgtcggg gtcacggcgt
      661 ccacagcgtc tacagcgtcg aggtcgtcgg gcggccttct cctccatctc ggcagcgcgg
      721 cggggcggca caaccgtacc gcgttgttgc tggcacggtt ggcctgatcg ccgggttgtg
      781 cgcgagcgcg tgctgcctgc ctcgtttcca cctggtgcca acgggtatgc gcgagggcgt
      841 ccgcgccggg gccttccccc gatgaccgct ccggcctgcc tcggagaagg gcgatgtcgg
      901 agacgctggc gtccgtggtg gaccggttcg accggctgtc ggtgctcgtg ctcggcgagt
      961 cgatcctcga cgtgtacctg cggggcagct cgacacggct ctgccgggag gcgccggtgc
     1021 ccgtggtcga cgtggccggc cgggccgagg cgcccggcgg ggccgccaac gcggcggtga
     1081 acgcccgcgc cctcggcgcg cgggtgacct tcctgtcggt ggtcggcgac gaccgggagg
     1141 gcgcggcggt gctggcgctc ctggccgagg ccggcgtcga cgcccggcgc gtgctcgtcc
     1201 ggccaggccg gcggacgctc gcgaagcacc gggtggtcgc ggacggccag gtcctcgtcc
     1261 ggttcgacga gggcggaggc ggcgcgaccg acgagggctc ggacgccgag ctggccgagc
     1321 tgctcgccgc cgaggcggcc gggcacgacg ccgtgctggt gtcggactac gggtacgggg
     1381 tgctcggagc ggccgtcgcc ggtgtgctgc gccggctgca ggccgaggcc ccgagggtcc
     1441 tggtcgtgga cgcgaggcac ccgacggcgt accgcggcgt cggggcgacc gcggtgaagc
     1501 cgaactacga cgaggcggtg cagctcctcg gcggcggccg cttcgaccct cgcggcagcc
     1561 gcgccgacca gctcgagcgc cagggccggc gcatcctctc gctgctcggc gccgaggtcg
     1621 ccgcggtgac cctcgacacc gagggcgcgg tgctgttcga gcgggacggg ccgccgtacc
     1681 ggacctatgc gcggccggcg gccagcgcgc gggcggccgg cgccggggac acgttctcga
     1741 gcgcgctcac gctcgccctg gcggccggcg cgggcgtgcc cgcggccggc gagctcgcct
     1801 cggccgcggc cgccgtcgtg gtggccgcgg acggcacggc ggtgtgcccg cgggcggcgc
     1861 tcgaggaggc ggtgggcggc gaccgccgca tcgtcaccgc ggccgagctc gccgcccggg
     1921 cgagcctcta tcggcggcag gggcgccgca tcgtcttcac caacggctgc ttcgacatcc
     1981 tgcaccgggg ccacatcacc tacctcaacc gggccaagac cctcggggac gtgctggtgg
     2041 tgggggtcaa cgccgacgcg ggcgtccggc ggctgaaggg tccggaccgc cccctgaacg
     2101 ccctggacga ccggatggcg gtcctcgccg ccctgagctg catcgaccac ctggtcgcgt
     2161 tcgaggacga caccccggcg gagctgatcc gggcggtcag gccggacgtg ttcgtcaagg
     2221 gcggggacta ccggcgcgac accgtccccg aggggccgct ggtcgagtcg ctcgggggcg
     2281 cggtggagat cctgcccttc ctggaggccc gctcgaccac cggcatcgtc gagcgcatcc
     2341 gggcgggcgg gccgccccgc gccggccggc gcgggccgcg ggagcgctcg gcatgagcgc
     2401 cgcgggagcg ctcggcgcga gcgccgccgg cgcccgcctc gtccccggcg tcgccaggct
     2461 cgcggtgctg cgggccaacg cggtcgggga cttcctgctc gccctgccgg cgctcgaggc
     2521 cctgagaagg gcgtacccgg acgccgaggt cgtgctgctc ggctcggact ggcaccgggc
     2581 cttcctcgcc gggcggccaa gcccggtcga ccgctgcgtc gccgtccccc cgagccgcgg
     2641 ggtgcgcgac gaccgcccgc ccgcgccgcc ggaggcgctc gacgcgttct tcgcccggat
     2701 gcgcgccgag cggttcgacc tcgccctgca gctccacggc gggggggcca gctccaaccc
     2761 gttcgtggcc cgcctcggcg cggcggccac ggcgggggcg cgcgcggcgg acgcccccgg
     2821 cctcgaccgg accaccgcct acaacctgta ccagccggag gtcctgcgcc tcctcgaggt
     2881 ggtctcgctg gtcggcgccg cgccggtcac gctcgagccg agggtggcgg tcaccgccgc
     2941 cgaccgggcc gagagccgcg ccgcgctacc gggcgacggc cggcccctgg tcgccatcca
     3001 ccccggcgcc cgggacccgc gccgccgctg gccgcccgag cggtt
//
LOCUS       DASPYY010000173         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1436884, whole genome shotgun sequence.
ACCESSION   DASPYY010000173 DASPYY010000000
VERSION     DASPYY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436884"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VEP73_02595"
     CDS             complement(<1..901)
                     /locus_tag="VEP73_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT25359.1"
                     /translation="MNTLIVGDHFIGPEAFQEAVARVLGPDFGPVRTVRWAGADAAEQ
                     HAAQQVMEKDGPEAVPTPDEVVEAAGEAEVLLVHFAPVPKAVLEAAPRLKAVVVAPGR
                     VRERECRGGHPAGRGGRQRARPQRRRGRRADPRPAARRAPRHRKGRRRHQGGRLARGV
                     GRPGPRAGRQDGRDDRHVARQLARRLSGFRVRLLVHDPYVDPHTIDALGGEKVADLDG
                     VFREADAVTLHARLTDETRRFIGRRQFALMKPTAYFVNTARSRMVDEDALYEALRERR
                     IAGAALDVHDGEPLPPDSPWRALG"
     gene            1161..2582
                     /locus_tag="VEP73_02600"
     CDS             1161..2582
                     /locus_tag="VEP73_02600"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT25360.1"
                     /translation="MSSVGTVGDTLSGRTRIVVVGAGFAGFHCLRRLEKLLPPEAAEI
                     VVVSATDYMPYLPLLPEVGAGIVDPRHIAVSLSASLGRTRLVPGHAVGVDFAARTCEV
                     EDPEGRRRAIGWDRLVLAPGSVTRTLPVPGIAEHAVGFKNIAEALYLRDHLLAQLELA
                     DATDDLAERRARCTFVVVGGGYTGTEVAAQGQLFTRAARPHYPHIGPDDLRWILVDLA
                     PAILSELGERLGRPALSTLRRRGLDVRLRTTVEEATGNSVRLSDGTTVPTHTLVWCVG
                     VTPGPLTSALGLKTVKGRIVVDEYLTVPGHREVLAAGDAAAVPDLARPGGLTPMTAQH
                     AQRQGKAAARNLAASLGYGTRHAYRHRDLGFVVDLGGWQAVANPLHVPLSGVPAKVVT
                     RAYHLLALPANRLRVATDWFNDVVEHRQFVQLGLVREPQARLAAAEPQDVYYRSDGRP
                     LGDGSHVDTVPADAPPRSPSGTP"
     gene            complement(2566..>3051)
                     /locus_tag="VEP73_02605"
     CDS             complement(2566..>3051)
                     /locus_tag="VEP73_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase"
                     /protein_id="HYT25361.1"
                     /translation="DVVATLPVVDAQLGVPTPNPQFWHRYNRDGYGEQPDGGPWDIGF
                     PPAPLDSPWGSQATIGRIWPIFAGERGEYELAAGQPAAARLAAMAATGNDGHLLPEQV
                     WDAFPPSGSDGFTPMTGTFSATPLAWSHAQFIRLAWSIATGAPVEQPSIVACRYVRAC
                     R"
BASE COUNT          380 a         1116 c         1151 g          404 t
ORIGIN      
        1 tgccgagcgc ccgccatggg ctgtccggcg gcagcggctc gccgtcgtgg acgtcgagcg
       61 ccgcgccggc gatgcgccgc tctctcaggg cctcgtagag cgcgtcctcg tcgaccatgc
      121 ggctgcgcgc cgtgttcacg aagtaggcgg tcggtttcat gagggcgaac tgccgcctgc
      181 cgatgaagcg cctggtctcg tcggtcaggc gggcgtgcag ggtgacggcg tccgcctccc
      241 ggaagacgcc gtccaggtcg gcgaccttct ccccgccgag cgcgtcgatc gtgtgcgggt
      301 cgacgtaggg gtcgtggacg agcaggcgga cgcggaagcc ggacaggcgg cgggcgagct
      361 ggcgggcgac gtgccgatca tcccgaccgt cctgccgccc agctcgtggc cccggccggc
      421 cgactcctcg ggccagccgc ccgccttgat gccggcgtcg gcccttgcga tgccgcggag
      481 ctcggcgagc agcaggccga gggtcagctc ggcgaccgcg gcggcgttgc ggccgagcac
      541 gctgacgacc gccacgcccc gccgggtggc ctcctcgaca ttcacgttct cgtacccggc
      601 ccggggccac caccacggcc ttgagcctgg gggcggcctc gaggacggcc ttgggcaccg
      661 gcgcgaagtg aacgagcagg acctcggcct caccggccgc ctcgacgacc tcgtccggcg
      721 tggggacggc ctcggggccg tccttctcca tgacctgctg ggcggcgtgc tgctcggccg
      781 cgtccgcccc ggcccagcgc accgtgcgca ccgggccgaa gtccgggccg agcacccggg
      841 cgaccgcctc ctggaacgcc tcgggcccga tgaagtggtc accgacgatc agcgtgttca
      901 tgtccctccc tcgcccccag gcgcaggccg gcagggcggc catcgcccgg ggccagtagg
      961 tggccaggtt gaccgcggcc agcgccagca gcggggcgag gcacgttccg gtgagcctac
     1021 ccggcaatcg tccagaccca accggccagc cactgtaggg ttagatggcg cgcggcgcgc
     1081 aacccataca agccaggcca acccgcacac ggtgcggttc ggggcccggc gcgacgggcc
     1141 ttccacgtct gcagaagggg gtgagctcgg tcgggacggt cggggacacg ttgagcgggc
     1201 ggacgcggat cgtcgtggtc ggggctggct ttgccggctt ccactgcctg cgccggctgg
     1261 agaagctgct cccgccggag gcggcggaga tcgtcgtcgt gtcggcgacc gactacatgc
     1321 cgtacctgcc cctgctgccg gaggtcggcg ccggcatcgt cgacccgcgg cacatcgcgg
     1381 tctcgctctc ggcgtcgctg gggcggaccc ggctcgtccc tgggcacgcg gtgggcgtgg
     1441 acttcgccgc ccgcacgtgc gaggtggagg acccggaggg ccgccgccgc gcgatcggct
     1501 gggaccgcct ggtgctggcc ccgggcagcg tgacgcggac gctgcccgtg ccgggcatcg
     1561 cggagcatgc ggtcgggttc aagaacatcg ccgaggcgct ctatctccgt gatcatctgc
     1621 tggcacagct ggagctcgcc gacgccaccg acgaccttgc cgagcggcgc gctcgctgca
     1681 cgttcgtcgt ggtcggcggc ggctacaccg gtacggaggt cgccgcgcag gggcagctgt
     1741 tcacccgggc cgcccggccg cactacccgc acatcggccc ggacgacctg cggtggatcc
     1801 tggtcgacct ggccccggcg atcctgtccg agctgggcga gcggctcggc cgcccggcgc
     1861 tctcgaccct gcgccgccgc ggcctggacg tgcggctgcg gacgacggtc gaggaggcca
     1921 ccgggaacag cgtgcggctg tcggacggca ccacggtccc gacgcacacg ctggtgtggt
     1981 gcgtgggcgt gaccccaggg ccactgacca gtgcccttgg cctgaagacg gtgaagggcc
     2041 ggatcgtcgt ggacgagtat ctgaccgtgc ccggccaccg ggaggtgctc gcggccgggg
     2101 acgcggccgc cgtccccgac ctggcccggc ccggggggct caccccgatg accgcccagc
     2161 acgcgcagcg tcagggcaag gccgcggcgc gcaacctcgc ggcgtcgctc ggctacggca
     2221 cgcggcacgc gtaccggcac cgcgacctcg gcttcgtggt cgacctcggc ggttggcagg
     2281 cggtcgccaa ccccctgcac gtgccgctgt ccggcgtgcc ggcgaaggtg gtcacccgcg
     2341 cgtaccatct gctcgccctg cccgcgaacc ggctgcgggt ggcgaccgac tggttcaacg
     2401 acgtcgtcga gcaccggcag ttcgtccagc tcgggctggt ccgcgagccg caggcgcggc
     2461 tggcggcggc cgaaccgcag gacgtgtact accgcagcga cggacgcccg ctcggggacg
     2521 gcagccacgt ggacacggtt ccggcggacg ccccgccccg ttctcctagc ggcacgccct
     2581 gacgtaccgg caggcgacga tcgacggctg ctcgaccggc gcgcccgtgg cgatcgacca
     2641 ggcgagccgg atgaactggg catgcgacca cgccagcggg gtggccgaga acgtcccggt
     2701 catcggcgtg aagccgtcgc tgccggacgg cgggaaggcg tcccagacct gctctggcag
     2761 gaggtggccg tcattgccgg tagcggccat cgcggcgagc ctggccgccg ccggctggcc
     2821 ggcggcgagc tcgtactcgc cccgttcacc ggcgaagatg ggccagatcc gaccgatggt
     2881 ggcctggctg ccccaggggc tgtccagcgg cgccggcggg aagccgatgt cccacggtcc
     2941 gccgtcgggc tgctcaccgt agccgtcccg gttgtaccgg tgccagaact gcgggttggg
     3001 cgtgggcacg ccgagctggg cgtcgaccac gggcagggtg gcgacgacgt c
//
LOCUS       DASPYY010000174         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_733157, whole genome shotgun sequence.
ACCESSION   DASPYY010000174 DASPYY010000000
VERSION     DASPYY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733157"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VEP73_02610"
     CDS             complement(<1..466)
                     /locus_tag="VEP73_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYT25362.1"
                     /translation="MNEPVVVEIEVAAPTEVVWAFLTDPDELVRWLGVAATLEVHPGG
                     TFRFELFEGQFCSGRYVEVVPYRRVVFTWGWEDQAIPVPPGSTTVAIDLEPRLDQRTL
                     VRLTHAGLDWAMRPLHADGWSRYLERLTAVAEGRTPPDDPSRAYQGARAVPNP"
     gene            complement(463..768)
                     /locus_tag="VEP73_02615"
     CDS             complement(463..768)
                     /locus_tag="VEP73_02615"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT25363.1"
                     /translation="MAAIANPVRRRMLELVWDAERTPSDLAERLGLTRPATSQHLRVL
                     RDAGLVEARVEGGHRYYAVREEQLQALREFLEGFWGSRLGRLRTAAEQHQRRSRGDS"
     gene            complement(884..1306)
                     /locus_tag="VEP73_02620"
     CDS             complement(884..1306)
                     /locus_tag="VEP73_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25364.1"
                     /translation="MLNRTQALVLGFFLMVWGSLVVIRAAAPDVYDQALKLPPSWPRW
                     AGIAFLIGLAAFIALLSIGVLRRWRWAFWLILVAFLSGVLRVPVAILQLTGVLPANVP
                     TWYVVFQGLIGLAQLAIGLAMLAGYRCSGVWGASGRVA"
     gene            1558..>2867
                     /locus_tag="VEP73_02625"
     CDS             1558..>2867
                     /locus_tag="VEP73_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25365.1"
                     /translation="MLVAVALANTVFFSVPLGEARARVALYLALTMTPFAVLSPLVGP
                     WLDRRPGSYRVAIVAAMAGRVALAILLSSRSEHLALYPLAFGLLVLSRVHGVSRSALV
                     PEARPPGRSLMWANARVSVISVAAGTAGAGPALAINHWVGTSATLWTAAVVYAAGALA
                     ATGLPLGESESEGGGEARRPRGRVRRYQELLTPRLLAGGLAMATLRSAVGFVTFLLAF
                     LLRAEGEGGRGLAVAAAAAAAGGFAGSVAAPALRAVLQEPVMLLASLAVAAATAVWAA
                     FGFGLLRAGILAGMIGLAAGAGRLAFDSLLQKDAPGHVRARTFARYETIFQLCWVAAA
                     GVAAAVPFGPGAGLWSLGGLCACGLVLSAWELAHSAGMAPAPPRLGLSDRLRALPGVL
                     RVLPGVLRGLLDRRRQARAARSAAAPARPDGETPPAPEAPGGPAV"
BASE COUNT          337 a         1089 c         1055 g          386 t
ORIGIN      
        1 gtgggttcgg gacggcgcgg gctccctgat aggcacgtga cgggtcgtcg ggcggcgtcc
       61 ttccctcggc gaccgcggtg agccgttcca ggtatcggct ccacccgtcg gcgtgcagcg
      121 gtcgcatcgc ccagtcgaga ccggcatggg tcagccggac gagcgtccgc tggtcaaggc
      181 gcggttcgag gtcgatggcg accgtggtcg agccgggcgg gacggggatc gcctggtcct
      241 cccaccccca ggtgaacacc acccgccggt acggaaccac ctcgacgtag cggccggagc
      301 agaactgccc ttcgaacagc tcgaaccgga aggtgccgcc cgggtgtacc tcgagcgtcg
      361 ccgcgacccc aagccagcgg accagctcgt ccgggtcggt caggaacgcc cagaccacct
      421 ccgtcggcgc ggcgacctcg atctcgacca cgacgggctc gttcacgagt ctcctctcga
      481 acgtcgttga tgctgctcgg cggccgtccg caaccttccc agccggcttc cccagaagcc
      541 ctccaggaac tcgcggagtg cctggagctg ctcctcgcgg acggcgtagt agcggtggcc
      601 gccttcgacc cgcgcctcga cgaggcccgc gtcccgcagc acccgcaggt gctgggaggt
      661 ggcagggcgc gtcagcccca gccgttcggc gaggtcggac ggcgtccgct cggcgtccca
      721 caccagctcg agcatgcgcc ggcggacagg gttcgcgatc gcggccaagg cctgttccat
      781 gaggttagtt aagcagatct gcaattgcgt gacaacgcaa caggtcgggc gtcctgccgc
      841 ccgccgacac gacacccttg gcgcctgggc gcggttggcg agctcaagcg acccgcccgg
      901 acgcccccca gacgccggag caccggtagc cggccagcat ggcgagtccg atggcgagct
      961 gggccaggcc gatgagcccc tggaacacga cataccaggt tgggacattg gcgggcagga
     1021 caccggtgag ctgcaagatg gccaccggca cgcgcagcac cccggacagg aaggcgacca
     1081 ggatcagcca gaaggcccag cgccatcgcc gcagcacgcc gattgacagc agcgcgatga
     1141 aggcagcgag cccgatcagg aacgcgatcc ccgcccaccg cggccagctg ggcgggagct
     1201 tgagcgcctg gtcgtagacg tcgggcgctg ctgcgcggat cacgaccagg ctgccccaca
     1261 ccatgaggaa gaagccgagg accagcgcct gcgtccggtt gagcatgcgt gcccgtcctc
     1321 tcggggtgtc gtgtgcctcc gctcggccac gagtatgacc ccacccatcg gaaccgcacc
     1381 gtgcggcggt gcgccacgct tccccggccc ggcggcccgc cgcatactgg ggccatgacc
     1441 tcgcctgccg gctggccgcc ggcacacgcg ccagggacag gggcccgcgc gccgagctgg
     1501 cgctggcgag ccttccggcg tctcctcgag acccaggccg cgcacgccgc cggggacgtg
     1561 ctcgtcgccg tggccctggc caacacggtg ttcttctccg tcccactggg ggaggccagg
     1621 gccagggtcg cgctctacct cgccctcacc atgaccccct tcgcggtcct gtcgccgctg
     1681 gtcggcccgt ggctcgaccg gcggcccggc tcctaccggg tggcgatcgt cgccgcgatg
     1741 gccggccggg tcgcgctcgc gatcctgctc tccagcaggt cggagcacct ggcgctctac
     1801 ccgctcgcgt tcggcctgct ggtcctgtcc cgcgtgcacg gcgtcagccg cagcgcgctc
     1861 gtcccggagg cgaggccgcc gggccgctcg ctgatgtggg ccaacgcccg ggtgtcggtg
     1921 atctcggttg ccgcgggcac ggccggtgcc ggcccggcgc tcgccatcaa ccactgggtc
     1981 gggaccagcg ccaccctgtg gacggccgcg gtcgtctatg ccgccggcgc gctggccgcg
     2041 accgggctgc cgctcggcga gagcgagagc gaaggcgggg gcgaggcgag gcggccgcgc
     2101 gggcgggtca ggcgctacca ggagctgctc accccccggc tgctggcggg cgggctcgcc
     2161 atggccacgc tgcgctccgc ggtcgggttc gtcacgttcc tgctcgcctt cctgctccgg
     2221 gccgagggcg agggcggcag gggcctcgcg gtcgcggcgg ccgccgccgc ggccggcggg
     2281 ttcgccgggt cggtggccgc gccggcgctc cgggccgtgc tgcaggagcc ggtcatgctg
     2341 ctggcgtcgc tggcggtcgc ggcggccacg gccgtctggg cggccttcgg cttcggcctc
     2401 ctgcgcgcgg ggatcctcgc cggcatgatc ggcctcgcgg ccggcgccgg ccggctcgcc
     2461 ttcgacagcc tgctgcagaa ggacgcgccc ggccacgtgc gggcccggac cttcgcccgc
     2521 tacgagacca tcttccagct ctgctgggtg gcggcggcgg gcgtggccgc cgccgtcccg
     2581 ttcgggccgg gcgccggcct gtggtccctc ggggggctct gcgcgtgcgg cctcgtgctg
     2641 tcggcctggg agctcgccca ctccgcgggc atggccccgg caccgccccg cctcgggctc
     2701 tccgaccggc tgcgcgcgct gcccggcgtc ctgcgcgtgc tgcccggcgt cctgcgggga
     2761 ttgctcgacc gccggcggca ggcccgggcg gcccggtcgg cggccgcgcc cgcccgtccg
     2821 gacggcgaga cgccgcccgc tcctgaggcg cccggtgggc ccgcggt
//
LOCUS       DASPYY010000175         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1997763, whole genome shotgun sequence.
ACCESSION   DASPYY010000175 DASPYY010000000
VERSION     DASPYY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997763"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VEP73_02630"
     CDS             <1..386
                     /locus_tag="VEP73_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYT25366.1"
                     /translation="RQLATLSVGEAVLIGSAQILAFLAGISRSGATMVAGLARGLDHE
                     DAARFSFLLATPIILAAGVYKLPDLLGPNGDGVRGQVLAGSAAAAVAAYLSVRFLTRW
                     FQTRTLTPFAIYCLVAGVACIVRFA"
     gene            587..>2052
                     /locus_tag="VEP73_02635"
     CDS             587..>2052
                     /locus_tag="VEP73_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYT25367.1"
                     /translation="MHPRSPRRLPGTPLALLALLALALLSSPAVPEARAGLGETARPL
                     PTAAGGPAGTSLPVIVRKTAPGSTEAERLVRELGGTVVRELPIVGGFAARVQAARVPL
                     LLGSPAVRRVWPDGRVRMRGLDGGPDAHDPAPALLAWRKAIHLDGLGRGGTRATVALL
                     DTGITRTPDLGDRVRVRVDLTEEHDGYDRYGHGTHLSGIIAGNGEMMDGSAGGRRGGG
                     YQGAAPSARLVSVKVADGSGATDVSVVLAGLQWVVSHRDRYGIKVLNLSFGTDSTQSY
                     AIDPLDEAVERVWRSGILVVVAAGNDGELGPGRISKPADDPFVLTVGAADLEGTERAG
                     DDVVAPFSGRGPTADGIAKPDLVAPGTSILGIRAEGSTIDRFRPAARYGLHYLKGTGT
                     SQAAAVVSGVAALLYDADPGLRPDVAKAALVHTAQRQLDGQPGAGAGLVDARAAVQAA
                     ERGTYESRPANQGLRRSTGLGSLEASRGSQHAYTDLDG"
BASE COUNT          245 a          799 c          746 g          262 t
ORIGIN      
        1 gccggcagct cgcgaccctc tcggtcggag aggcggtcct gatcgggtcg gcgcagatcc
       61 tggcgttcct ggccgggatc agccggtccg gggcgacgat ggtggccggt ctggcgcgcg
      121 gcctcgacca cgaggacgcc gcccgcttct cgttcctgct cgccaccccg atcatcctgg
      181 ccgccggggt ctacaagctg ccggacctgc tcggccccaa cggcgacggg gtccgcggcc
      241 aggtcctggc cggcagcgcg gccgccgcgg tggcggccta cctgtcggtg cggttcctca
      301 cgcgctggtt ccagaccagg accctgaccc cgttcgcgat ctactgcctg gtcgccggcg
      361 tggcctgcat cgtccgcttc gcgtgagcag cggccgtccg cgcgacggct caggacccgg
      421 ccaaccacag acaatccagg catcgactgg catggtccgg cgccggcacg ggtcggcgcc
      481 gttagcgttc cgttggcgcc ggaggcgatc tataggcgcg acctcctgaa ccctccgacg
      541 tgacacagga gcaggctcta tgtcatggca ggtgcctcgc gtccccttgc acccccgatc
      601 gccccgaagg ctcccaggaa cgccgctggc gctgctcgca ctgcttgcgc tggcgctcct
      661 ctcctccccc gccgttcccg aggcccgggc cggcctcggc gagacggccc ggccgctgcc
      721 gaccgcggcg ggcgggccgg cgggcacgtc gcttccggtc atcgtccgca agaccgctcc
      781 cggctcgacc gaggcggaac ggctggtgcg cgagctcggc gggaccgtgg tccgcgagct
      841 gccgatcgtc ggcggcttcg cggcccgcgt gcaggccgcg cgcgtgcccc tgctgctcgg
      901 ctccccggcg gtgcggcggg tctggcccga cggccgtgtc cgcatgcgcg gcctcgacgg
      961 cggcccggac gcccacgacc ccgcgcccgc cctgctggcc tggcgcaagg ccatccacct
     1021 cgacgggctg ggccggggcg gcacccgcgc caccgtcgcc ctgctcgaca cgggcatcac
     1081 ccggaccccg gacctgggcg accgggtgcg ggttcgggtg gacctcaccg aggagcacga
     1141 cggctacgac cgctacgggc acggcaccca cctcagcggc atcatcgccg gcaacggcga
     1201 gatgatggac gggagcgcgg gcggccggcg aggcggcggg taccaggggg ccgccccctc
     1261 ggcccggctc gtctcggtga aggtggccga cgggagcggg gccaccgacg tctcggtcgt
     1321 gctggcgggc ctgcagtggg tggtgtccca ccgcgaccgc tacggcatca aggtcctgaa
     1381 cctgtcgttc gggaccgact cgacccagag ctacgcgatc gaccccctcg acgaggcggt
     1441 cgagcgggtg tggcgctcgg gcatcctggt ggtcgtcgcg gccggcaacg acggtgagct
     1501 cggacccggc aggatctcca agcccgccga cgaccccttc gtgctcaccg tcggggcggc
     1561 cgacctcgag ggcaccgaac gggccggcga cgacgtggtc gcgccgttct ccggacgggg
     1621 ccccaccgcc gacggcatcg ccaagccgga cctggtggcg ccgggcacct cgatcctcgg
     1681 catccgcgcc gagggcagca cgatcgaccg cttccgtccc gccgcgcggt acggcctcca
     1741 ctacctgaag ggcacgggca cgtcccaggc cgccgcggtc gtctccggcg tcgccgcgct
     1801 gctctacgac gcggaccccg gcctgcgacc ggacgtggcc aaggcagccc tggtccacac
     1861 ggcgcaacgc cagctcgacg gccagcccgg cgcgggcgcc gggctggtcg acgcccgggc
     1921 ggcggtccag gccgccgagc gcgggaccta cgagtcgcgc ccggcgaacc agggtctgcg
     1981 gcgctcgacc ggcctcggct ccctggaggc gagccggggc tcgcagcacg cgtacactga
     2041 cctcgacggc ga
//
LOCUS       DASPYY010000176         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_874023, whole genome shotgun sequence.
ACCESSION   DASPYY010000176 DASPYY010000000
VERSION     DASPYY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874023"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..192)
                     /locus_tag="VEP73_02640"
     CDS             complement(40..192)
                     /locus_tag="VEP73_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25368.1"
                     /translation="MPEYLAAARRIAEASAAAQGYPPTVTDESVLYQLARLLERQGGD
                     GHADVA"
     gene            complement(452..1900)
                     /locus_tag="VEP73_02645"
     CDS             complement(452..1900)
                     /locus_tag="VEP73_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYT25369.1"
                     /translation="MTAGIYGRVSIHRDAGDAEQHDVETSVGRQFRDCLDYASRQGWT
                     VARTYKDEGISAYSGADRPDFNRLVADVDAGIVEAVICWKLDRLCRSHADFQRLWAVI
                     ERRGARLVSLHEMFDSSTAAGEFVVRLMVGMAKMESQNISLRVKSYLDAAARAGRPRH
                     GGARGYGYTYQGKLVPGEAAHVRWAAAELLTGRSLRSVVAELNARGATGTKGRAFDRR
                     QLVRVLTAARVAGLKEHGGEVVGPGDWEPILARADWERLVALLTDTGRRTHDGRPRVY
                     LLGGFLRCGVQGCGMLLISRPQWRKGGTTRRYVCVQGDTAAGRAHLAVAAEPLEDVVT
                     EMVLHRVDGPGLARILAARGTNGEQEVAAQLAADEQAMVALSEDFYSARLITRAEFLA
                     NRATLEGRIRAARSKLARHAESAAAAGLPTAPGAIRDAWPGWTGPDGLERRRSVLALM
                     LDHVVVGPAPRRGVHEFDPSRVEPPYGPVWRA"
     gene            complement(1836..2495)
                     /locus_tag="VEP73_02650"
     CDS             complement(1836..2495)
                     /locus_tag="VEP73_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT25370.1"
                     /translation="MQTAIEARDLVRRFGDVEAVRGVSLEVGEGEIYGFLGPNGAGKS
                     TLVRMLTTLLAPTSGTGAVLGHDVAREAATVRRLIGVALQEAGLDPKQTGRELLTLQG
                     RLYGLKGRARARQVEEVTELVGLTGAIDRQIKGYSGGMKRRLDLASSLLHRPRVLFLD
                     EPTTGLDPTSRTLIWDRMRTLRSDYGATIFLTVMYSTCYDRGYLRASQHPQRRWRRRA
                     A"
     gene            complement(2575..>3981)
                     /locus_tag="VEP73_02655"
     CDS             complement(2575..>3981)
                     /locus_tag="VEP73_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HYT25371.1"
                     /translation="PTVAESLNAAGMAALLGDPGQRAAHVQALVGLVTANGYDGVDLD
                     YESMNFGGTATDKATVRSGFTALAGELGAALHALKDDVHPQGRLLSVSVGPRTKADDP
                     NWSVHDYAGLAPSVDRFRVMAYNYHWGGGPPGPIAPVWWVDQVLAYAVTAVPAGKIEV
                     GAPLYGYDWPADPTQPDGYGTATGRTYAQAEALRTQYGATRQWSSVDVVGRPVAAPYF
                     AYTTPEGVRHVVWYNDADATKAKMTFIEKYHLRGLVFWSAGYEDARQWDALRAYAIQK
                     STALAVRAPARVTYGTRIAVSGRLATTSGAAVPGQRVVLQWRAAGSTTWRSIATGTTS
                     STGTVAFGSTPKANGAYRLVAPSSWSYLSSTSPAVTTLVQWRVSAAFYDATVRRGTTV
                     RLHGGVAPIRPGTTVRRQRYVNGSWVTVASTTVHSDGTYAFLFAPSTAGTYVYRVLVP
                     GTALNATGNSPTLKLYVS"
BASE COUNT          504 a         1407 c         1506 g          564 t
ORIGIN      
        1 cgagtcgccc cggttcccga gccggggcgc tctcttttct cacgcgacgt cggcatgacc
       61 gtcaccgccc tgccgctcga gcaggcgcgc gagctggtac aggacgctct cgtccgtgac
      121 ggtcgggggg tagccctgcg cggcggccga cgcttcggcg atgcggcgcg cggcggcgag
      181 gtactcgggc atggctcgcc ttcccaggcg gtcccggggc gggcggcgcg acggcctcta
      241 ctgctcgggc ggtactgctc tatgacttgc ggtcatgtac ccgcagggat aagacttcgc
      301 ctcgagacgt acgtctcagc gccgtaggtg gcccctacgg ggtgttgcct gtcgtcgcgt
      361 gccgcagcgc ggtcggaggg accggtcaag tctgcctacc gcgctgcggc gcactgtaaa
      421 gcagaatgcc gcgcgcttcc attagcgcag gtcaggcacg ccagaccggc ccgtacggcg
      481 gctcgaccct gctcgggtcg aactcgtgta cgccgcgccg gggcgccggg ccgacaacca
      541 catggtcgag catgagcgcg agcaccgacc ggcgccgctc gaggccgtcc gggccggtcc
      601 agcccggcca ggcgtcgcgg atggcgccgg gcgcggtcgg caggccggcc gcggcggcgc
      661 tctcggcgtg ccgggcgagc ttggaccggg cggcgcggat gcggccctcg agcgtggccc
      721 ggttggcgag gaactcggcc cgggtgatga ggcgggccga gtagaagtct tccgagagcg
      781 cgaccatggc ctgctcgtcg gcggcgagct gcgcggcgac ttcctgctcg ccgttggtcc
      841 cccgggccgc gaggatgcgc gcgaggcccg gcccgtcgac gcggtgcagc accatctcgg
      901 tgacgacgtc ctcgagcggc tcggcggcga cggcgagatg cgcccggccg gcggccgtgt
      961 cgccctgcac gcacacgtag cggcgggtcg tgccgccctt gcgccactgc gggcggctga
     1021 tgagcagcat gccgcacccc tggacgccgc agcgcaggaa cccgccgagc aggtagacgc
     1081 ggggccgccc gtcgtgggtg cgccggccgg tgtcggtgag cagcgcgacg agccgttccc
     1141 agtccgcccg ggcgaggatg ggttcccagt cgcccgggcc gaccacctcg ccgccgtgct
     1201 ctttgaggcc ggcgacccgg gccgcggtca gcacgcgcac gagctggcgg cggtcgaacg
     1261 cgcggccctt ggtgccggtc gcgccgcggg cgttcagctc ggcgacgacc gaccgcaggg
     1321 accggccggt gagcagctcg gcggcggccc agcggacgtg cgcggcctcg ccgggcacca
     1381 gcttgccctg gtaggtgtag ccgtacccgc gggcgccgcc gtggcgcggc cggccggccc
     1441 gggcggcggc gtcgaggtag ctcttgaccc gcagcgagat gttctgcgat tccatcttgg
     1501 ccatgccgac catgagccgg acgacgaact cgcctgcggc ggtcgacgag tcgaacatct
     1561 cgtgcaggct gactaggcgg gcgccgcggc gctcgatgac ggcccacaag cgctggaagt
     1621 cggcgtgcga gcggcagagg cggtcgagct tccagcagat gacggcctcg acgatacccg
     1681 cgtcgacgtc ggcgacgagc cggttgaagt cgggccggtc ggcgcccgag tacgcgctga
     1741 tgccctcgtc cttgtaggtc ctggcgaccg tccagccctg ccggctggcg tagtcgaggc
     1801 agtcccggaa ctgccggcct accgaggtct cgacgtcatg ctgctcggcg tcgccagcgt
     1861 ctctgtggat gctgactcgc ccgtagatac ccgcggtcat agcaggtact atacatgact
     1921 gtgaggaaga tcgtcgcgcc gtagtcgctg cgcagcgtcc gcatccggtc ccagatgagc
     1981 gtccgggagg tggggtcgag cccggtcgtg ggctcgtcca ggaagagcac cctgggccgg
     2041 tgcaggagcg acgaggcgag gtcgagccgc cgcttcatgc cgcccgagta gcccttgatc
     2101 tgccggtcga tcgcgccggt caggccgacc agttccgtca cctcctcgac ctgcctggcc
     2161 cgcgcccgcc ccttgagccc gtagaggcgg ccttggaggg tgagcagctc gcggccggtc
     2221 tgcttggggt cgaggccggc ctcctgcagg gccaccccga tgagccgccg cacggtcgcg
     2281 gcctcccgtg cgacgtcgtg gccgagcacc gcgcccgtcc cggaggtcgg cgcgagcagg
     2341 gtggtcagca tgcggacgag ggtcgacttg ccggcgccgt tcggcccgag gaacccgtag
     2401 atctcgcctt ccccgacctc gaggctgacg ccgcggaccg cctcgacgtc gccgaaacgg
     2461 cgcaccaggt ctcttgcttc gatcgctgtt tgcatgcaga caagtctaga ggcgacctgt
     2521 gacagccggc ctgggacacc gccacggtcc ccgccgaggc tccggcgggc cgggtcagga
     2581 cacgtagagc ttcagggtcg ggctgttgcc ggtggcgttg agcgccgtgc cggggaccag
     2641 cacccggtag acgtaggtgc cggccgtgga cggggcgaac aggaacgcgt aggtgccgtc
     2701 ggagtgcacc gtggtcgacg cgaccgtgac ccaggagccg ttgacgtagc gctggcgccg
     2761 caccgtcgtg cccggccgga tcggcgcgac gccgccgtgc aggcgcacgg tcgtgccgcg
     2821 gcgcaccgtg gcgtcgtaga aggccgcgga gacgcgccac tgcaccagcg tggtgaccgc
     2881 cgggctggtg gaggagaggt aggaccacga cgacggcgcg acgagccggt aggcgccgtt
     2941 ggccttcggc gtggagccga aggccaccgt gccggtcgag gacgtggtgc cggtcgcgat
     3001 gctccgccac gtcgtggacc cggcggcccg ccactgcagc acgacccgct ggccggggac
     3061 cgccgcgccc gacgtcgtgg cgagcctgcc cgacacggcg atcctcgtcc cgtaggtgac
     3121 cctggccggg gcccgcaccg cgagggcggt gctcttctgg atggcgtacg cccgcagggc
     3181 gtcccactgc cgtgcgtcct cgtagccggc ggaccagaac accaggcccc ggaggtggta
     3241 cttctcgatg aaggtcatct tcgccttcgt cgcgtccgcg tcgttgtacc agacgacgtg
     3301 ccgcacgcct tcgggcgtgg tgtaggcgaa gtacggcgcc gcgaccgggc ggccaaccac
     3361 gtccacgctc gaccactgcc tggtggcgcc gtactgcgtg cgcagcgcct cggcctgcgc
     3421 gtaggtcctc cccgtggcgg tgccgtaccc gtcgggctgc gtcgggtcgg ccggccagtc
     3481 gtagccgtac agcggcgcgc cgacctcgat cttgccggcg ggcacggcgg tcacggcgta
     3541 ggcgaggacc tggtcgaccc accagacggg tgcgatgggg ccgggcggcc cgccgcccca
     3601 gtggtagttg taggccatga cccggaaccg gtcgaccgag ggcgcgaggc cggcgtagtc
     3661 gtggaccgac cagttcgggt cgtcggcctt cgtgcgcggc ccgacggaca ccgacagcag
     3721 cctgccctgc gggtgcacgt cgtccttgag cgcgtgcagc gcggccccga gctcgccggc
     3781 gagcgccgtg aagccgctgc gcacggtggc cttgtcggtc gccgtgcccc cgaagttcat
     3841 cgactcgtag tcgaggtcga cgccgtcgta gccgttcgcg gtgaccaggc cgacgagcgc
     3901 ctgtacgtgc gcggcgcgct gcccggggtc gccgagcagc gccgccatgc cggcggcgtt
     3961 cagcgactcc gcgacggtcg g
//
LOCUS       DASPYY010000177         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2560688, whole genome shotgun sequence.
ACCESSION   DASPYY010000177 DASPYY010000000
VERSION     DASPYY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560688"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VEP73_02660"
     CDS             <1..742
                     /locus_tag="VEP73_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYT25372.1"
                     /translation="YGPCGGEVSVHAWGPGAAWCLETAGDLLGGRDRLDGFEPRGLVR
                     ELHRRLPGLRISRSLTVFEALVPSVLEQKVTGQEAHASYRAVVRAWGTPAPGPVPLLV
                     PPGPDVLASRAYWEFHRFGVEMKRANTIRAAAACAARLEEAAGMPAPDAHRRLQVVPG
                     VGPWTAAEVAIVALGDADAVSVGDYHLPHLVSWSLAGEPRGTDERMLELLAPYAGHRG
                     RVLRLLTRAGRSAPRFGPRMPLRSFRRF"
     gene            complement(827..1657)
                     /locus_tag="VEP73_02665"
     CDS             complement(827..1657)
                     /locus_tag="VEP73_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYT25373.1"
                     /translation="MPEGDTVFLAATRLHQALAGRRLLATDFRIPAFATADLAGQAVR
                     EVTPRGKHLLLRTDAAVTLHTHFKMEGAWHLYRPAERWRGPDFQVRAVLRTEPWVAVG
                     FRLAICELLPTDREHEVVGHLGPDVLGPDWDLDEAAGRLRADPDRAVGTALLDQRAIA
                     GPGNVWKCELCFLWGVDPWTPVGEVRDLEGMLALLERMMDANRTTGCQITTGDTRPGR
                     TSWVTARTGKPCRRCGTPIRRAEQESYGAERPTYWCPTCQPGPGPARQPRRRARPWAR
                     "
     gene            complement(1663..>1901)
                     /locus_tag="VEP73_02670"
     CDS             complement(1663..>1901)
                     /locus_tag="VEP73_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25374.1"
                     /translation="LTFTDDADRLGRAADALALAARDGALGRLSVEKADGETVLDSPL
                     AQALTAAGFRPSTRGLRLSGGPPGVRPAPRTGGA"
BASE COUNT          187 a          707 c          772 g          235 t
ORIGIN      
        1 ctacggcccg tgcggcggcg aggtctccgt gcacgcgtgg gggccgggcg ccgcctggtg
       61 cctcgagacc gcgggcgacc tgctcggggg gcgcgaccgg ctggacggct tcgagccgcg
      121 ggggctggtc cgggagctgc accgccgcct tcccggcctg cgcatcagcc ggtccctgac
      181 ggtgttcgag gcgctcgtcc cttcggtcct cgagcagaag gtgaccggcc aggaggcgca
      241 cgcctcctac cgcgcggtcg tccgcgcctg gggcacgccg gcgcccgggc ccgtgccgct
      301 gctggtgccg cccgggccgg acgtcctcgc cagcagggca tactgggagt tccaccggtt
      361 cggggtggag atgaagcgtg ccaacacgat ccgggcggcc gccgcctgcg cggcgcggct
      421 cgaggaggcg gccggcatgc ccgcgcccga cgcgcaccgg cggctgcaag tggtccccgg
      481 cgtgggcccg tggaccgcgg ccgaggtggc catcgtcgcc ctcggcgacg ccgacgcggt
      541 ctccgtgggc gactaccacc tgccccatct ggtgagctgg tcgctggccg gggagccgcg
      601 cgggaccgac gagcggatgc tcgagctgct cgcaccctac gccgggcacc gggggcgggt
      661 gctgcggctg ctcacgcgcg cggggcggtc ggcgccgcgc ttcggcccgc ggatgccgct
      721 gcggtcgttc cggcggttct gaccacccgc cgcgggtcgc gccgcgcctt ggcgccggcc
      781 ccgtgctccg ggtccgtggg cggcccgtgc cgctggtccg ccggggtcag cgcgcccacg
      841 ggcgggcgcg ccgacggggc tggcgggcgg gaccggggcc gggctggcag gtcgggcacc
      901 agtacgtcgg gcgctctgcg ccgtagctct cctgctcggc cctgcggatc ggggtcccgc
      961 agcggcggca gggcttgccg gtccgcgcgg tcacccagct cgtccggccg gggcgggtgt
     1021 cgccggtggt gatctggcag ccggtggtcc ggttggcgtc catcatccgc tcgagcaggg
     1081 cgagcatgcc ctcgaggtcc cgtacctcgc cgaccggcgt ccacgggtcg acgccccaca
     1141 ggaagcagag ctcgcacttc cacacgttgc ctgggccggc gatcgcccgc tggtccagca
     1201 gtgcggtgcc gaccgcccgg tccgggtcgg cgcgcagccg cccggccgcc tcgtcgaggt
     1261 cccagtccgg ccccagcacg tcggggccga ggtggccgac gacctcgtgc tcacggtcgg
     1321 tgggcaagag ctcgcagatc gcgagccgga agccgacggc gacccagggc tcggtgcgca
     1381 ggacggcgcg gacctggaag tccggtcccc gccagcgctc ggccggccgg tacaggtgcc
     1441 aggccccctc catcttgaag tgggtgtgca gggtcacggc cgcgtcggtc cgcagcagca
     1501 ggtgcttgcc tctgggcgtc acctcgcgga cggcctggcc ggcgaggtcg gcggtggcga
     1561 acgccgggat ccggaagtcg gtcgcgagca ggcgccggcc ggccagcgcc tggtgcagcc
     1621 gggtcgcggc caggaagacg gtgtcgccct cgggcacggc gctcaggccc cgcccgtgcg
     1681 gggggcgggc cgcacgcccg gcgggccccc ggacagccgc aggccgcggg ttgatgggcg
     1741 gaagccggcc gcggtgagcg cctgggccag cgggctgtcc agcaccgtct cgccgtcggc
     1801 cttctcgacc gagagccggc cgagggcgcc gtcgcgggcg gccagggcga gcgcgtccgc
     1861 cgcgcgcccg agccggtcgg cgtcgtcggt gaaggtgagc a
//
LOCUS       DASPYY010000178         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2139242, whole genome shotgun sequence.
ACCESSION   DASPYY010000178 DASPYY010000000
VERSION     DASPYY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139242"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="tmk"
                     /locus_tag="VEP73_02675"
     CDS             <1..836
                     /gene="tmk"
                     /locus_tag="VEP73_02675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYT25375.1"
                     /translation="MDDRRGVPLREDLMAAIRSESPPPIGEPGHVNGSTPPGPPPGRL
                     IAFEGGEGSGKTTQARLVAIWLRELGYDVVTTHEPGATKIGMRLRALLLDTSHTGMSP
                     RAEALMYAADRAEHVASVIAPALARGAIVITDRYVDSSLAYQGAGRNLPVDEIAGFNR
                     WATGGRTPDLTILLDMDPMAGLSRRARSADRLEAEPADFHLRVRAGFQALARAEPARY
                     LVLDADRPPAEITRDIQERIRELLPDPVPSAAEASTGDFPAIRAEVLTPTPQPGDRRR
                     P"
     gene            833..>1738
                     /locus_tag="VEP73_02680"
     CDS             833..>1738
                     /locus_tag="VEP73_02680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018727564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYT25376.1"
                     /translation="MTGVFADLPGQDAVVAQLRRAAAGAAALLAGQRTEPGAMTHAWL
                     FTGPPGSGRSVAARAFAAALLCDEGGCNQCNSCRQVFAGTHADLLLVRPEGLSYGVKQ
                     TRDLVLRAAGKPTGGRWQIVLFEDADRCTEQAANALLKAIEEPAPQTVWLLCAPSAED
                     LVPTIRSRCRVVTLVVPSSAAVAQALTERDGIEPARALAAARAAQGHIGRARRLAVDP
                     AAAKRRADVLRVPSLATSLGPALSAAATLVRTAEEEAKAVTEELDEPEREALRRAFGE
                     GSSGKGVAKAMRGMAGAMKDLEDRQK"
BASE COUNT          214 a          680 c          641 g          203 t
ORIGIN      
        1 agatggacga ccgcaggggc gtgccgctgc gcgaggacct gatggcggcg atccgcagcg
       61 agtcgccccc gccgatcggc gagcccggcc atgtcaacgg cagcaccccg cccggaccgc
      121 cgccggggcg gctgatcgcg ttcgagggcg gcgagggctc gggcaagacc acgcaggccc
      181 ggctggtcgc catctggctg cgcgagctcg gctacgacgt ggtgaccacg cacgagcccg
      241 gcgcgaccaa gatcggtatg cggctgcgcg cgctgctcct ggacacctcg cacaccggca
      301 tgtcgccgcg tgccgaggcg ctgatgtacg cggccgaccg ggccgagcac gtcgcctcgg
      361 tcatcgcgcc cgcgctggcc cgcggcgcga tcgtcatcac cgaccggtac gtcgactctt
      421 ccctggccta ccagggcgcg ggccggaacc tgccggtcga cgagatcgcc gggttcaacc
      481 ggtgggcgac cggcgggcgc accccggacc tcacgatcct gctggacatg gacccgatgg
      541 cggggctgag ccgccgggcc cgttcggccg accggctcga ggccgagccc gccgacttcc
      601 acctccgggt ccgggccggc ttccaggccc tggcccgcgc cgaacccgcc cgctacctgg
      661 tgctcgatgc cgaccggccg cccgccgaga tcacccgcga catccaggag cggatccgcg
      721 agctgctccc cgacccggtg ccctcggccg ccgaggccag caccggtgac ttccccgcga
      781 tccgggccga ggtcctgacc cccaccccgc agccgggcga ccggcgccgc ccgtgaccgg
      841 ggttttcgcc gacctccccg gccaggacgc ggtcgtcgcc cagctccgcc gggccgccgc
      901 cggggccgcc gccctgctgg ccggccagcg caccgaaccc ggcgcgatga cccacgcctg
      961 gctgttcacc ggcccgcccg ggtcaggccg gtcggtggcg gcccgggcct tcgccgccgc
     1021 gctgctgtgc gacgagggtg gctgtaacca gtgcaacagc tgccgccagg tcttcgccgg
     1081 cacccacgcc gacctgctgc tggtccggcc cgaggggctg tcctacgggg tgaagcagac
     1141 ccgcgacctg gtgctgcgcg cggcgggcaa gccgaccggc gggcgctggc agatcgtcct
     1201 gttcgaggac gccgaccggt gcacggaaca ggccgccaac gccctgctca aggcgatcga
     1261 ggagcccgcc ccgcagaccg tctggctgct ctgcgcgccc tcggccgagg acctggtgcc
     1321 cacgatcagg tcgcgctgcc gggtggtcac gctcgtcgtg ccgtcgtcgg cggccgtggc
     1381 tcaggcgctg accgagcggg acggcatcga gcccgctcgc gcgctggccg cggcgcgtgc
     1441 ggcgcagggg cacatcggcc gcgcccggcg tctcgcggtt gatcccgccg cggccaagcg
     1501 ccgcgccgac gtgctgcgcg tcccctccct ggcgacctca ctggggcccg cgctgtccgc
     1561 ggccgcgacc ctggtgcgca ccgctgagga ggaggccaag gcggtcaccg aggagctcga
     1621 cgagccggag cgcgaggcgc tgcgccgcgc gttcggcgag ggatcctcgg gcaagggcgt
     1681 ggccaaggcg atgcgcggca tggccggtgc catgaaggac ctggaggacc ggcagaag
//
LOCUS       DASPYY010000179         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_874788, whole genome shotgun sequence.
ACCESSION   DASPYY010000179 DASPYY010000000
VERSION     DASPYY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874788"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1184
                     /gene="thiL"
                     /locus_tag="VEP73_02685"
     CDS             123..1184
                     /gene="thiL"
                     /locus_tag="VEP73_02685"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HYT25377.1"
                     /translation="MTAPGTPGGPGASPPSPSRTELAALGEFGLIDRLTAGPSPGAEA
                     GVEVGVGDDAAVLVPTPGRRLVVTTDVMVEGRHFTAELSSPEDWGWKAVAVNLSDLAA
                     MGAEPRWLVVALTVPPTAPLATLEGVYAGMREACRTLGGVLVGGDTSGGPVLSLAVTA
                     IGEAERAVTRSGARPGDRLVVTAPLGAAAAGLALLELAAGHGGDPAAREAARDLLARH
                     PHLAAAHRRPRPELAAGRRLAAGGATAMLDLSDGLAGDVLHVAEASGTGVEVRDAAVP
                     LAAGVAEAAGLLGRAPVELALGGGEDFALAAALPRTADVGGVIDCGTFTDRPDRRVHL
                     TVAGEALPLAGLAYDHFRA"
     gene            complement(1245..>1853)
                     /locus_tag="VEP73_02690"
     CDS             complement(1245..>1853)
                     /locus_tag="VEP73_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYT25378.1"
                     /translation="PEAFITAHDAMFSQAGLRMSETLVVHGAAGGVGTAAVQLAVTAG
                     ARALAVVRSGAVAEAVAALGAEVVREDAFPGAVQEATGGAGVDVILELVGSPHFLANL
                     EVLALKGRVVVVGVAAGQDTALPLRVLMQRRATVLGTVLRARPLEEKATAVRAFEREV
                     VPQLAAGRVRPVVDSEFPAGEVVAAFERLAGHGKLGKVLLAF"
BASE COUNT          202 a          750 c          704 g          197 t
ORIGIN      
        1 ctcgaccatc acgtcggtgg tgaccacgag ccgccggccc ggcgtgggga cgaggacggc
       61 ggcgtcgtcg ccgacgccga cctcgacccc ggcctcgacg aggcgatcac gaaggccgtc
      121 acctgaccgc tccgggcacg ccggggggac ccggggcgag ccctccttcg ccgtccagaa
      181 cggagctcgc ggcgctcggc gagttcgggc tcatcgaccg gctcaccgcc gggccgtccc
      241 ctggcgccga ggccggggtc gaggtcggcg tcggcgacga cgccgccgtc ctcgtcccca
      301 cgccgggccg gcggctcgtg gtcaccaccg acgtgatggt cgagggccgg cacttcaccg
      361 ccgagctgtc cagcccggag gactggggct ggaaggcggt cgcggtcaac ctgtccgacc
      421 tcgccgcgat gggcgccgag ccacgctggc tggtggtcgc cctcactgtg ccgcccaccg
      481 ccccgctggc cacgctcgag ggggtgtacg ccggcatgcg ggaggcctgc cggacgctcg
      541 gcggggtcct ggtcggtggc gacacgtccg gcggcccggt cctgtcgctg gccgtcaccg
      601 ccatcggcga ggccgagcgg gcggtcaccc ggtccggtgc ccgcccgggc gaccggctcg
      661 tcgtcaccgc gcccctgggc gcggccgccg ccggactcgc gttgctcgag ctcgcggccg
      721 ggcatggcgg cgaccctgcc gcgcgcgagg cggccaggga cctgctcgcc cgccaccccc
      781 acctggccgc cgcccaccgc cggccccgcc cggagctcgc cgccggccgg cgcctggccg
      841 cgggcggcgc caccgccatg ctcgacctgt ccgacgggct tgccggcgac gtgctccacg
      901 tcgccgaggc gtccggcacg ggcgtcgagg tccgcgacgc ggccgtgccg ctcgccgccg
      961 gggtcgccga ggcggccggg ctgctcggcc gcgcccccgt cgagctcgcg ctcggcgggg
     1021 gagaggactt cgccctggcc gccgccctgc cccgcacggc cgacgtcggc ggggtgatcg
     1081 actgcggcac cttcaccgac cggcccgacc gccgcgtcca cctgaccgtg gccggcgagg
     1141 cgctccccct ggccggcctc gcctacgacc acttccgcgc ctgaccggcg cgacctaccc
     1201 ggggccggga ggcgcccgac gctggcgata gcctccggag cccgtcagaa cgccagcagg
     1261 accttgccga gcttgccgtg gccggcgagg cgctcgaagg cggccaccac ctcgccggcc
     1321 gggaactccg agtcgacgac cggccgcacc cggcccgccg cgagctgcgg cacgacctcg
     1381 cgctcgaacg cgcgcacggc ggtcgccttc tcctcgagcg gccgggctcg cagcacggtc
     1441 ccgagcacgg tcgcgcggcg ctgcatcagg acgcgaaggg gcagcgcggt gtcctggccg
     1501 gcggccacgc cgaccacgac gacccgtccc ttgagcgcga gcacctcgag gttggccagg
     1561 aagtggggcg acccgacgag ctcgaggatc acgtccacgc cggcgccgcc ggtcgcctcc
     1621 tggaccgcgc cggggaaggc gtcctcgcgg accacctcgg cgccgagcgc ggcgacggcc
     1681 tcggcgaccg cgccggagcg gacgacggcg agcgcccggg cgccggcggt caccgcgagc
     1741 tggacggcgg cggtgccgac cccgccggcg gcgccgtgga cgacgagggt ctcgctcatg
     1801 cgcagcccgg cctgggagaa catggcgtcg tgggcggtga tgaacgcctc ggg
//
LOCUS       DASPYY010000180         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2139705, whole genome shotgun sequence.
ACCESSION   DASPYY010000180 DASPYY010000000
VERSION     DASPYY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139705"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VEP73_02695"
     CDS             <1..650
                     /locus_tag="VEP73_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT25379.1"
                     /translation="AREHCVALGLEPERCRVVLPGVDTELFHPPAEPVREPVAAFVSP
                     LAPNKGIDRVLEAFRLVRRRLPEAELRVAGRGPLEGLVRREAARQGSGVRFLGAVDRD
                     GVARELRRTAVFVTAPRPTRVWNEQFGLAYVEAMASGLAVVTTACGSNHEAVRPPNER
                     VPDDAEALAEALLGFLADPARRVRVGEENRRVALDRHELRRQCARMGEAFRLAGG"
     gene            complement(682..1527)
                     /locus_tag="VEP73_02700"
     CDS             complement(682..1527)
                     /locus_tag="VEP73_02700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT25380.1"
                     /translation="MSGGPPRPPLVSVVVPTRDSGRTIEACLRSVRSQTHPAVELIVV
                     DNSSSDGTWPAALRLADAALRAGPERSAQRNAGIARAAGDWVLWIDSDMELAPDVVER
                     SLAAAREAGATGVFIPEASTGSGFWARCRALERRCYTGEPLVEAPRLVRTDYLRRTGG
                     FAVWLAGTEDAELRNRMLADGARLAGADTRIVHDEGRLSLAAVLRKRFYYGKGLPAYR
                     RAHPGAVSAQAGATARAIWRHRRLFAADPVHAGGLAVLRACEAAAWAAGAAAGVGRGR
                     GSPPA"
     gene            complement(1524..1958)
                     /locus_tag="VEP73_02705"
     CDS             complement(1524..1958)
                     /locus_tag="VEP73_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25381.1"
                     /translation="MIRRLLAAAVPVAVLWFLGGSIGLLAGAAMAAWDLLRQPPARHL
                     LRASVLLFALVPVAVLARGLPSPATVGPAFASRDLVAHGLAGAALALLVLGILREVRP
                     PSALPAAGDPPGGRALATRDGAPALAWPGEPAGEGPAPRGDR"
     gene            complement(1955..>2512)
                     /locus_tag="VEP73_02710"
     CDS             complement(1955..>2512)
                     /locus_tag="VEP73_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25382.1"
                     /translation="GNLARLGGGGDLAVALCPGGPPLRLGAGEHRLRAEPGWLVDLLD
                     LSSTSGGGGRALEAAGTPPAPPRITVTSSSPTRTTLVTEAAHGPYYLVLGQGWDSRWR
                     ATIDGLPLGPPAVVDGYSVGWRIPDTRAHLVAVEFTPQRWAQRSLLASLAGLALVLAL
                     LAGVGRRRPATAGAGATPRGEVERR"
BASE COUNT          292 a          986 c          995 g          239 t
ORIGIN      
        1 cagcccgcga gcactgcgtc gcgctcggtc tcgagccgga gcgctgtcgc gtcgtgctcc
       61 ccggggtcga caccgagctg ttccacccgc ccgccgagcc ggtgcgcgag ccggtggccg
      121 cgttcgtctc gccgctcgcc cccaacaaag gcatcgaccg ggtgctggag gcgttccggc
      181 tcgtccggcg gcgcctgcca gaggcagagc tgcgggtcgc gggccggggc ccactcgagg
      241 ggctggtccg ccgcgaggcg gcccgccagg gcagcggcgt ccgcttcctc ggggccgtcg
      301 accgggacgg ggtcgcgcgc gagctccgcc ggacggcagt cttcgtcacc gccccccgcc
      361 cgaccagggt ctggaacgag cagttcgggc tcgcctacgt cgaggcgatg gccagcggcc
      421 tcgcggtcgt cacgaccgcg tgcggctcca accacgaggc cgtccggccc cccaacgagc
      481 gcgtgcccga cgacgccgag gccctcgccg aggcgctgct cgggttcctc gccgacccgg
      541 cgaggcgcgt ccgggtcggc gaggagaacc ggcgcgtggc gctcgaccgg cacgagctgc
      601 gccgccagtg cgcgcgcatg ggcgaggcgt tccgcctcgc gggcgggtag gaccagaccg
      661 ggccggccag gctcgcgggg atcaggctgg cggggacccg cggccccgcc cgacgcctgc
      721 cgccgcgccg gccgcccagg ccgccgcctc gcacgcgcgc aggacggcca ggccccccgc
      781 gtggacgggg tcggcggcga acaggcggcg gtggcgccag atcgcgcggg cggtcgcgcc
      841 ggcctgcgcc gagaccgcgc ccggatgggc ccgccggtag gccgggagcc ccttgccgta
      901 gtagaagcgc ttgcgcagca cggcggccag ggacagccgt ccctcgtcat gcacgatccg
      961 cgtgtcggcc cctgccagcc tcgccccgtc ggccagcatg cggttgcgca gctcggcgtc
     1021 ctcggtgccg gcgagccaca cggcgaagcc gccggtccgg cgcaggtagt ccgtgcgcac
     1081 gagcctgggc gcctcgacca gcggctcgcc ggtgtagcag cgtcgctcga gggcgcggca
     1141 gcgggcccag aaccccgagc cggtgctcgc ctccgggatg aacacgccgg tcgcccccgc
     1201 ttccctggcg gcggcgagcg accgctcgac cacgtcgggc gcgagctcca tgtcggagtc
     1261 gatccacagc acccagtcgc ccgcggcccg ggcgatgccg gcgttgcgct gcgcgctgcg
     1321 ctccggcccg gcccgcaggg ccgcgtcggc caggcgcagg gccgccggcc aggtgccgtc
     1381 cgagctggag ttgtcgacca cgatcagctc gacggccggg tgcgtctggg accgcacgga
     1441 gcgcaggcag gcctcgatcg tccgtcccga gtcgcgcgtc ggcacgacca ccgagacgag
     1501 cggcggccgc ggcggcccgc cgctcatcgg tccccccgcg gcgccggccc ctcgcccgcg
     1561 ggctcgcccg gccaggcgag cgccggcgct ccgtcgcgcg tggcgagggc acgccctcct
     1621 ggcgggtcgc ccgctgccgg gagtgcggac ggcggccgca cctcgcgaag gatgcccagg
     1681 acgaggagcg cgagtgcggc gccggccagg ccgtgcgcga ccaggtcgcg gctcgcgaac
     1741 gccgggccca ccgtcgcggg cgagggcagg ccgcgcgcga ggacggcgac cggcaccagc
     1801 gcgaacagca gcaccgacgc ccgcaggagg tgcctggccg gcggctggcg gaggaggtcc
     1861 catgcggcca tcgccgcgcc ggccagcagc ccgatcgatc ccccgaggaa ccacagcacg
     1921 gcgaccggca ccgccgcggc caggagccgc cggatcaccg gcgctccacc tccccgcgtg
     1981 gcgtggcgcc ggcacccgcc gtggcgggtc ggcgccgtcc cacgccggcg agcagggcga
     2041 gcacgagggc gagcccggcc agcgaggcga gcagggagcg ctgggcccag cgctgggggg
     2101 tgaactcgac cgcgaccagg tgggcgcggg tgtccgggat gcgccagccg accgagtagc
     2161 cgtccaccac ggcgggcggg ccgaggggca ggccgtcgat cgtggcgcgc cagcgggagt
     2221 cccagccctg gccgaggacc aggtagtagg ggccgtgcgc ggcctcggtg acgagcgtgg
     2281 tccgggtcgg cgaggaggag gtgaccgtga tgcgcggcgg cgccgggggc gtgccggccg
     2341 cctcgagcgc ccggccgccc cctcccgacg tggaggacag gtcgagcagg tcgacgagcc
     2401 agccgggctc ggccctgagc cggtgctccc cggccccgag gcgcagcggc ggcccccccg
     2461 ggcacagcgc caccgcgagg tcaccgccgc cgccgagccg ggccaggttc cc
//
LOCUS       DASPYY010000181         7385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2561255, whole genome shotgun sequence.
ACCESSION   DASPYY010000181 DASPYY010000000
VERSION     DASPYY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561255"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VEP73_02715"
     CDS             <1..543
                     /locus_tag="VEP73_02715"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HYT25383.1"
                     /translation="CLALQDALERHGVVTRVQTAIQMTQIAEPYIPRRAVRHLEKGRI
                     VIFGAGMGAPFFSTDTTAAQRALEIGADAILMAKNGVDGVYDADPNKHPGARKFSRLD
                     YLEAISRELKVMDTTALSLCKDNGLPIVVFDLLTEGNIRRAVCGEPIGTIVGPMADSV
                     PVEPASQRGAGEDRERGEEG"
     gene            540..1097
                     /gene="frr"
                     /locus_tag="VEP73_02720"
     CDS             540..1097
                     /gene="frr"
                     /locus_tag="VEP73_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYT25384.1"
                     /translation="MIEDTLNEAETKMGKAIEVAKEEFSGIRTGRANPALLHRLTVEY
                     YGTPTPLQQLASISAPEARLLVVAPYDRNATSSIEKAIRNSDLGINPSNDGQVIRLAF
                     PPLTEERRKEYVKLTRHRAEEARTSIRNVRRHEKDALERLEKDGQISQDELRRAELRL
                     QQVTDRYVAEVDTMLQHKERELLEV"
     gene            1161..2054
                     /locus_tag="VEP73_02725"
     CDS             1161..2054
                     /locus_tag="VEP73_02725"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYT25385.1"
                     /translation="MADNPPLDPADGQPPADGTVPRRRRTAGRNLPVAIATGLGLAGG
                     LIAALVLAPPVFIAFLLGIVVLALVELVNVLRARATRPARPVVLPMAVVLIVGAYLEG
                     PAALSFGLVVTLLAAFFWYLVDPGRTEVTRNVAATVFAVLYVPFLAAHLALVVSQVDH
                     YVGAIIGYALVVVVYDTAAYATGVMIGRHPLAPHVSPSKSWEGAAGATLVCLLTGALA
                     LPLWRPWTLASGLTLAAVACVAAPLGDLAESMLKRDLAVKDMGSILPGHGGVLDRIDA
                     LLFMAPALYYVLALYSLVKGS"
     gene            2051..3178
                     /gene="rlmN"
                     /locus_tag="VEP73_02730"
     CDS             2051..3178
                     /gene="rlmN"
                     /locus_tag="VEP73_02730"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYT25386.1"
                     /translation="MTPLRSPYDLSPSDLAALAAEAGQPAWRAGQLAHWLDRRRVTDP
                     LAMTDLPRGFRERLADRLAGTGLEPRRHQVGDGGWTHKLLFGLPGGDSVESVLMLYPG
                     RATVCVSTQAGCAMGCTFCATGQFGFTRNLSAGEVVEQVARVWAELPGLRPHPSAPGH
                     LTNVVFMGMGEPFANPAATWGAARRLHEGFGLSARAITISTVGLVPGIRRAAVEPLAV
                     NLAVSIHAPDDELRDRLVPVNRSWPIADLLDAVRGYVEATRRRVSFEYALMRGVNDGV
                     EQGRELGRLLAPLRVPGSGGHAAHVNVIPYNATPGTGFSAPSRSAVRRFRDAVAGSGV
                     PVSVRANRGAGIDAACGQLRTMAGRPGRAPAPPLVTPGTSG"
     gene            3175..4380
                     /gene="dxr"
                     /locus_tag="VEP73_02735"
     CDS             3175..4380
                     /gene="dxr"
                     /locus_tag="VEP73_02735"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYT25387.1"
                     /translation="MSGGNGAHGRPAGTGLAILGSTGSVGVQALDVVRSAGGRFRVVA
                     LAARRSAARLAAQARELRPAVVALADPTEAAELARDLPPGCRLEVGNEAVASLAACPG
                     VEVVLNGITGSAGLAPTLHALRAGRRLALANKESLIVGGELVTGQARDGQIVPVDSEH
                     SGLAQCLRAGRAGEVARLVVTASGGPFRGRPAAGLEAVTPREALAHPTWSMGPVISVN
                     SATLMNKGLEVIEAHLLFGIPFDRIDVVVHPQSIVHAMVEFTDGATIAQLSMPDMRLP
                     IGLALGWPARSAEPVGRVDWTRAMSLEFEPADRAAFPLLDLAVGAGRAGGTAPAALNA
                     ANEEAVAAFLAGRLPFPGIAEVVAGVLAAHEQPAELDLEAVLGAEQWSRGLARTLVED
                     HSVPGRPHE"
     gene            4373..5635
                     /locus_tag="VEP73_02740"
     CDS             4373..5635
                     /locus_tag="VEP73_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HYT25388.1"
                     /translation="MSSLLGILLFVFALMLAILFHEGGHFATAKLFGMKVERFFLGFG
                     TTLWSFRRGETEYGIKALPLGGFCKIAGMSPYESDGNFLEEERPRRGRRSAAAASTTG
                     TVIAGTLEPEAPKPAPAPPDRLFRNKPAWQRAIVLSAGSVTHFVVALVLIYAVLVGVG
                     LSVTTTRIAEVVPTAPSGDQAPAAAAGLRAGDRVVAVDNRPVDGFEQLRAAFADKAGR
                     PVTVTYERAGERRTTTLTPVAINDNGRVRGFLGVKGSVEKRRLGPVAGIGRTGSVFWS
                     AVTGSVKGLSQIVPGLTQRLRSTAGPDTATGGGGAAGGGSGESGPIGIVGIARFAGEA
                     VAANEWLAFLLLLIQFNVFVGLFNLLPIPPMDGGYLGFVAWEKLTRRTVDLRKVAPVA
                     AGIVILLFMLTVSLIWLDITNPVANPFK"
     gene            5714..6841
                     /gene="ispG"
                     /locus_tag="VEP73_02745"
                     /gene_synonym="gcpE"
     CDS             5714..6841
                     /gene="ispG"
                     /locus_tag="VEP73_02745"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYT25389.1"
                     /translation="MTDLAAGVGRRRSRRIDVGRVPVGGGAPISVQSMTITKTADVNA
                     TLQQIAELAATGCDIVRVAVPDTDDAAALPAIASHSTIPVVADIHFQWKYAMQALEAG
                     CAGVRINPGNIKKHDKVKLIAQEAAARAVPIRVGVNAGSLQKDILARHGGPTAEALVE
                     SAMFEVAILESVGFGAIKISVKHQNPATMIAAYRLLAERCDYPLHLGVTEAGTPRDGI
                     VKSAVGIGALLAEGIGDTIRVSLTAPPIEEVKAGNAILGALGLRPKKLDLVACPSCGR
                     AEVDVFALANAVEERMGEIDAPMRVAVMGCIVNGPGEAREADVGVASGKGKGQIFRHG
                     QVVCTVPEDEIVDALMHEAKTLAEELRAGGAGPGPAEVVRG"
     gene            6923..>7385
                     /locus_tag="VEP73_02750"
     CDS             6923..>7385
                     /locus_tag="VEP73_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HYT25390.1"
                     /translation="MLAFLDRAPVRDVLIASRILNDGALGPAGWSLLWGAFARHGGLR
                     GVLHVGPNVVPAVDDPSVCDALAPAARGPGPTRMLVGERVAVERLWRLVAASYPPPRE
                     VRARQYVYALAPGQLAAGPGGPPGGRARLATRADEDTVLRLSAAMYHEEMGE"
BASE COUNT          934 a         2783 c         2676 g          992 t
ORIGIN      
        1 tgcctcgccc tgcaggacgc cctggagcgc cacggggtcg tgacccgggt ccagaccgcg
       61 atccagatga cccagatcgc cgagccgtac atcccgagac gggcggtgcg ccacctcgag
      121 aaggggcgca tcgtcatctt cggggcgggc atgggggcgc ccttcttctc gaccgacacg
      181 acagcggccc agcgggcgct cgagatcggc gccgatgcga tcctcatggc caagaacggg
      241 gtcgacggcg tctacgacgc ggacccgaac aagcatccgg gggcgcgcaa gttctcccgc
      301 ctcgactacc tcgaggcgat cagccgtgag ctgaaggtga tggacaccac cgcactgtcg
      361 ttgtgcaagg acaacggcct gcccatcgtc gtgttcgacc tgctcaccga gggcaacatc
      421 cgccgggcgg tgtgcggtga gccgatcggg accatcgtcg gccccatggc cgactcggtg
      481 ccggtcgagc cggcgagcca gcggggcgcc ggggaggacc gggagagagg ggaagaggga
      541 tgatcgagga caccctgaac gaggcggaga ccaagatggg caaggccatc gaggtggcca
      601 aggaggagtt cagcggcatc cgcaccggcc gggcgaaccc cgcgctgctc caccggctca
      661 cggtcgagta ctacggcacc ccgaccccgc tccagcagct cgcctcgatc tcggcgcccg
      721 aggcgcgcct gctcgtcgtc gccccgtacg accgcaacgc gacctcctcg atcgagaagg
      781 cgatccgcaa ctccgacctc gggatcaacc cgtcgaacga cgggcaggtg atcaggctcg
      841 cgttcccgcc gctcaccgag gagcgccgca aggagtacgt gaagctgacg cggcaccggg
      901 ccgaggaggc gcggacgagc atccgcaacg tccgccggca cgagaaggac gcgctggagc
      961 ggctcgagaa ggacggccag atctcccagg acgagctgcg ccgcgccgag ctgcgcctgc
     1021 agcaggtgac cgacaggtac gtcgccgagg tcgacaccat gctgcagcac aaggagcggg
     1081 agctgctcga ggtgtgagca cgggcgacgg cccgccgtgc ggggaggtgc tggtggtcgg
     1141 aggtcctggg cgggggcggc gtggctgaca acccgccgct cgaccccgcc gacggccagc
     1201 cccccgccga cgggaccgtc ccgaggcgca ggcggacggc cgggcggaac ctgcccgtcg
     1261 cgatcgccac gggcctcggg ctggccggcg ggctcatcgc ggcgctggtg ctggctcccc
     1321 cggtgttcat cgccttcctg ctcgggatcg tcgtcctcgc gctggtcgag ctcgtgaacg
     1381 tgctgcgggc gagggccacg cgccccgccc gcccggtcgt gctgccgatg gcggtggtgc
     1441 tgatcgtcgg cgcctacctg gaggggcccg ccgccctgtc gttcgggctc gtggtcacgc
     1501 tgctcgccgc cttcttctgg tacctcgtcg acccggggcg caccgaggtc acccgcaacg
     1561 tcgccgcgac cgtgttcgcc gtcctgtacg tgccgttcct cgcggcgcac ctggcgctcg
     1621 tggtcagcca ggtggaccac tacgtcggcg cgatcatcgg ctacgcgctc gtcgtcgtcg
     1681 tgtacgacac cgcggcgtac gcgaccgggg tcatgatcgg gcggcacccg ctcgcgccgc
     1741 atgtgtcgcc gagcaagtcg tgggagggag cggccggggc gaccctcgtg tgccttctca
     1801 ccggcgctct ggcgctgccg ctgtggcggc cgtggacgct cgcgtccggc ctcacgctcg
     1861 ccgcggtcgc ctgcgtggcg gccccgctgg gggatctggc agagtccatg ctgaagcgcg
     1921 acctcgccgt caaggacatg ggctcgatcc tgcccggcca cggcggcgtg ctcgaccgta
     1981 tcgacgccct gctgttcatg gcgccggccc tctactacgt cctcgccctc tactcgctgg
     2041 tcaaaggctc gtgactccgc tgcgctcccc gtacgacctg tcgccgtccg acctcgccgc
     2101 cctggccgcc gaggccggtc agcccgcctg gcgcgccggc cagctggcgc actggctcga
     2161 ccggcgccgg gtcaccgacc cgctggccat gaccgacctg ccccgggggt tccgggagcg
     2221 gctggccgac cggctggccg ggaccggcct cgagccccgg cgccaccagg tcggggacgg
     2281 cggctggacc cacaagctcc tgttcgggct gccgggcggc gacagcgtcg agtcggtgct
     2341 catgctctac ccggggcggg ccacggtctg cgtctcgacc caggccggct gcgcgatggg
     2401 ctgcacgttc tgtgccaccg ggcagttcgg gttcacccgc aacctgtccg ccggggaggt
     2461 ggtcgagcag gtggccaggg tctgggccga gctgcccggg ctccggcccc atccttccgc
     2521 gccgggtcac ctcaccaacg tcgtgttcat ggggatgggc gagccgttcg ccaacccggc
     2581 ggcgacctgg ggtgccgccc ggcggctgca cgagggcttc ggcctgtccg cccgcgcgat
     2641 caccatctcg accgtcggcc tggtccccgg catccgccgg gccgccgtcg agccgctcgc
     2701 cgtcaacctc gccgtctcga tccacgcgcc tgacgacgag ctccgcgacc ggctcgtgcc
     2761 cgtcaaccgg tcgtggccga tcgccgacct gctcgacgcc gtccgcgggt acgtggaggc
     2821 gacccgcagg cgggtgtcgt tcgagtacgc gctcatgcgg ggcgtcaacg acggcgtcga
     2881 gcagggccgc gagctcggcc ggctgctcgc ccccctgcgc gtcccgggct cgggcgggca
     2941 cgccgcccac gtcaacgtca tcccctacaa cgcgaccccg gggaccggct tctcggcgcc
     3001 ctcgcggtcg gccgtgcgcc gcttccgcga cgcggtggcc ggctcgggcg tgccggtcag
     3061 cgtgcgcgcg aaccggggcg ccggcatcga cgccgcctgc ggccagctgc ggacgatggc
     3121 cggacggccc ggccgggcgc ccgccccgcc gctcgtgacc cccgggacca gcgggtgagc
     3181 ggcggcaacg gcgcgcatgg acggccggcc ggcaccgggc tcgcgatcct cgggtcgacc
     3241 gggtcggtcg gggtccaggc tctcgacgtc gtgcgcagcg ccggggggcg cttccgggtc
     3301 gtcgctctcg cggcgcgccg ctccgcggcc aggctggcgg cgcaggcccg cgagctccgt
     3361 ccggccgtgg tcgcgctcgc cgaccccacg gaagcggccg agctggcccg cgacctgccg
     3421 cccggctgcc gcctcgaggt cggtaatgag gcggtcgcga gcctggccgc ctgcccgggc
     3481 gtcgaggtgg tgctgaacgg gatcaccggg tcggccggcc tcgccccgac cctccacgcg
     3541 ctccgggcgg ggcggcggct cgccctcgcg aacaaggaga gcctgatcgt cggcggggag
     3601 ctcgtcaccg ggcaggcccg ggacggccag atcgtcccgg tcgactccga gcactccggg
     3661 ctcgcgcagt gcctgcgggc gggccgcgcc ggcgaggtgg cccggctggt cgtgaccgcc
     3721 tctggcgggc cgttccgggg ccgcccggcc gcagggctcg aggcggtcac gccgcgggag
     3781 gcgctcgccc accccacctg gtcgatgggc ccggtcatct cggtgaactc cgcaacgctg
     3841 atgaacaagg gcctcgaggt catcgaggcc cacctgctgt tcgggatccc gttcgaccgc
     3901 atcgacgtcg tcgtccaccc gcagagcatc gtgcacgcga tggtggagtt caccgacggc
     3961 gcgacgatcg cgcagctctc catgccggac atgcggctgc cgatcggcct tgccctcggc
     4021 tggcccgcgc gctcggccga gccggtcggg cgcgtcgact ggacgcgggc catgtcgctc
     4081 gagttcgagc cggccgaccg ggccgccttc ccgctcctcg acctcgccgt cggggccggg
     4141 cgggcagggg gcaccgcgcc cgccgcgctc aacgcggcga acgaggaggc ggtcgccgcg
     4201 ttcctggccg gccgcctgcc cttcccaggc atcgccgagg tggtcgccgg ggtcctcgcc
     4261 gcccacgagc agcccgccga gctggacctc gaggcggtcc tcggcgccga gcagtggtct
     4321 cgcggcctgg cccgtacact ggtcgaggac cattccgtac cagggagacc gcatgagtag
     4381 cctgctcggc atcctgctgt tcgtcttcgc cctcatgctg gccatcttgt tccacgaggg
     4441 cggccacttc gcgacggcga agctgttcgg gatgaaggtc gagcgcttct tcctcggctt
     4501 cggcacgacc ctctggtcgt tccggcgggg cgagaccgag tacggcatca aggcgctccc
     4561 gctcggcggc ttctgcaaga tcgccggcat gagcccctac gagagcgacg gcaacttcct
     4621 ggaggaggag cggccccggc gcggccggcg ctcggccgcc gccgcgtcga ccaccggcac
     4681 ggtgatcgcc gggacgctcg agccggaggc gccgaagccc gcccccgccc ctccggaccg
     4741 gctcttccgc aacaagccgg cgtggcagcg ggccatcgtg ctctcggccg gctcggtcac
     4801 ccacttcgtc gtcgccctcg tgctcatcta cgccgtgctc gtcggggtcg gcctcagcgt
     4861 gaccacgacc cggatcgccg aggtcgtccc caccgcgcca agcggcgacc aggcgcccgc
     4921 cgcggccgcc ggcctgcggg ccggcgaccg cgtcgtcgcc gtggacaacc gccccgtcga
     4981 cggcttcgag cagctgcggg ccgcgttcgc cgacaaggcg ggccgcccgg tgacggtcac
     5041 ctacgagcgg gccggcgagc gccgcaccac caccctcacg cccgtcgcga tcaacgacaa
     5101 cggcagggtc cgcgggttcc tcggcgtcaa gggcagcgtc gagaagcggc gcctcgggcc
     5161 ggtcgcgggc atcggcagga cgggatcggt gttctggtcc gcggtcaccg gctcggtgaa
     5221 gggcctctcc cagatcgtcc ccgggctcac ccagcgcctg cgctcgaccg cggggccgga
     5281 caccgcgacc ggcgggggcg gcgcggccgg gggcgggtcg ggcgagagcg gcccgatcgg
     5341 catcgtcggc atcgcgcgct tcgcgggcga ggcggtggcg gccaacgaat ggctcgcctt
     5401 cctgctcctg ctcatccagt tcaacgtctt cgtcggcctg ttcaacctgc tgccgatccc
     5461 gccgatggac ggcggctacc tcggcttcgt cgcctgggag aagctgacca ggcgaacggt
     5521 cgacctgcgc aaggtggccc cggtcgccgc cggcatcgtg atcctgctct tcatgctcac
     5581 cgtcagcctg atctggctcg acatcaccaa cccggtcgcc aaccccttca agtagcgcac
     5641 ccggcaaccg cccgggcacg ctcgcggccc gggccaggca aaggagctcg ttcccgtggc
     5701 cttcccgctc gagatgaccg acctggcagc cggggtcggg cgccgccgct cgcgccggat
     5761 cgacgtcggc cgggtgcccg tcggcggtgg cgcgccgatc agcgtccagt cgatgacgat
     5821 caccaagacg gccgacgtca acgccaccct gcagcagatc gccgagctgg ccgccacggg
     5881 ctgcgacatc gtccgggtgg ccgtgcccga caccgacgac gccgcggccc tgcccgccat
     5941 cgcgagccac tcgaccatcc cggtcgtcgc cgacatccac ttccagtgga agtacgcgat
     6001 gcaggcgctg gaggcgggct gcgccggcgt acgcatcaac ccgggcaata tcaagaagca
     6061 cgacaaggtg aagctgatcg cgcaggaggc ggccgcccgc gcggtgccga tccgggtcgg
     6121 cgtcaacgct ggatccctcc agaaggacat cctggccagg cacggcggcc ccaccgcgga
     6181 ggcgctggtc gagtcggcga tgttcgaggt cgcgatcctc gagtccgtcg gcttcggtgc
     6241 catcaagatc tcggtcaagc accagaaccc ggccaccatg atcgccgcct accggctgct
     6301 cgcggagcgc tgcgactacc cgctccacct cggcgtgacc gaggcgggca ccccgaggga
     6361 cggcatcgtc aagtccgccg tcggcatcgg cgcgttgctg gccgagggca tcggcgacac
     6421 gatccgggtg tccctcaccg cgcccccgat cgaggaggtc aaggcgggca acgccatcct
     6481 gggcgccctc ggcctgcggc ccaagaagct cgacctggtc gcctgcccgt cctgtgggcg
     6541 ggccgaggtg gacgtgttcg ccctcgccaa cgccgtcgag gagcgcatgg gcgagatcga
     6601 cgcgccgatg cgggtcgccg tgatgggctg catcgtcaac ggccccgggg aggcgagaga
     6661 ggccgacgtc ggcgtcgcct ccgggaaggg caagggccag atcttccggc acggccaggt
     6721 cgtctgcacc gtgcccgagg acgagatcgt cgacgccctg atgcacgagg ccaagacgct
     6781 cgcggaagag ctccgcgcgg gcggcgccgg gcccgggccg gccgaggtcg tccgcggcta
     6841 gatgcgctcg gcccgggttg gaggcggcgc cgcgtcgtcc ggcctcgcgg cacggacgct
     6901 cggtcgctcg gacgaggcgg ccgtgctcgc cttcctcgac cgcgcccccg tgcgggacgt
     6961 cctcatcgcc agccggatcc tgaacgacgg cgcgctcggc ccggccggct ggagcttgct
     7021 gtggggcgcg ttcgcccgcc acggcggcct ccgcggggtg ctccacgtcg ggcccaacgt
     7081 cgtgccagcg gtcgacgacc cgtccgtctg cgatgcgctc gccccggccg cccgcgggcc
     7141 cggcccgacc cggatgctgg tcggggagcg cgtcgccgtc gagcggttgt ggcgcctcgt
     7201 cgccgccagc tacccacccc cccgggaggt gcgcgccagg cagtacgtct acgccctggc
     7261 gccaggccag ctcgcggccg gaccgggcgg ccctccgggg ggccgggccc ggctcgccac
     7321 gcgggccgac gaggacaccg tcctgcgcct gtccgccgcg atgtaccacg aggagatggg
     7381 ggagg
//
LOCUS       DASPYY010000182         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1999288, whole genome shotgun sequence.
ACCESSION   DASPYY010000182 DASPYY010000000
VERSION     DASPYY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999288"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..>1501
                     /locus_tag="VEP73_02755"
     CDS             242..>1501
                     /locus_tag="VEP73_02755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT25391.1"
                     /translation="MSPNDNATTIPEHSFADLGVTPVTLTALARVGIEHPFPIQTLCL
                     PLALAGVDLIGQARTGTGKTLAFGVPIVQEVDPDDTVVQALVVVPTRELCLQVAGDVE
                     TAGRDAGIRVVSVVGGRPIGPQVVALSEGAQVVVGTPGRLLDLLRQRSLDLSQVRILV
                     LDEADEMLDLGFLPDVEAIIRATPDHRQTMLFSATMPKAVVALARRYSKQPTFVHAEH
                     GQEATVPATRQLFFQVHPLNRFQVLCRILDAPDRDRVAVFRRTKRGVDRTIKGLLDAG
                     YKAGALHGDLPQVVRERVMRAFRNGKLDVLVCTDVAARGLDIAGLSHVVNYDTPEDDR
                     AYVHRIGRTGRAGAAGVAITFVAWNELDTADLIRRRLGLTEEPLHEVYSTSPLLTELF
                     DLPTAEEAIARRTANGGRQGAPAAGGGS"
BASE COUNT          204 a          595 c          494 g          208 t
ORIGIN      
        1 ggcctggcgc agcgcctcgt acacctcggc cggaccggcc acggcggcta cctcctcggg
       61 gcgcgcgggg ctgcctgccc cgctgggacg cgatgcgccc cagagcctac cgcgctcctg
      121 acctccccgc ccaccggcgg cggtcggatc accgctatac tggttcgttg aatgcggccg
      181 acgccgtcct gcgcgggccg ctcaaggatc tttgatcttt ctcccacgac gcggaggctc
      241 gttgagtccg aacgacaacg ccacgaccat cccagaacac agcttcgccg acctcggtgt
      301 gacgccggtg accctcaccg cgctggcccg cgtcggcatc gagcatccct tccccatcca
      361 gaccctctgc ctgccgctgg cgctcgccgg cgtcgacctc atcgggcagg ccaggaccgg
      421 caccggcaag acgctcgcct tcggcgtccc gatcgtgcag gaggtcgacc cggacgacac
      481 cgtggtccag gccctcgtcg tggtcccgac ccgggagctg tgcctgcagg tggccggcga
      541 cgtcgagacc gccggccgcg acgccgggat ccgggtcgtg tccgtcgtcg gcggccggcc
      601 gatcggcccc caggtcgtcg ccctcagcga aggcgcccag gtcgtcgtgg gcaccccggg
      661 gcggctgctc gacctcctca ggcagcgcag cctcgacctc tcgcaggtcc gcatcctcgt
      721 cctcgacgag gcggacgaga tgctcgacct cggcttcctg cccgacgtcg aggcgatcat
      781 ccgcgcgacc cccgaccacc ggcagaccat gctgttctcg gccaccatgc cgaaggcggt
      841 cgtcgcgctc gccaggcgct actcgaagca gccgaccttc gtgcacgccg agcacgggca
      901 ggaggcgacc gtccccgcca ccaggcagct cttcttccag gtccacccgc tgaaccgctt
      961 ccaggtgctc tgccgcatcc tggacgcgcc ggaccgcgac cgggtcgcgg tcttccgccg
     1021 caccaagcgc ggtgtcgacc ggaccatcaa gggcctgctc gacgccggct acaaggccgg
     1081 cgccctgcac ggcgacctgc cccaggtggt ccgcgagcgg gtcatgcgcg cgttccgcaa
     1141 tggcaagctc gacgtgctcg tgtgcaccga cgtggccgcc cgcgggctcg acatcgccgg
     1201 cctgtcgcac gtcgtcaact acgacacgcc ggaggacgac cgtgcctacg tccaccggat
     1261 cggtcgcacg ggccgggccg gggcggccgg cgtcgcgatc accttcgtgg cgtggaacga
     1321 gctggacacg gccgacctca tccggcggcg cctcggcctg accgaggagc ccctgcacga
     1381 ggtctactcg accagccccc tgctgaccga gctgttcgat cttccaacgg cggaggaggc
     1441 gatcgccagg cggaccgcca acggcggccg ccagggtgcc cctgccgccg gcggcggcag
     1501 c
//
LOCUS       DASPYY010000183         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2281169, whole genome shotgun sequence.
ACCESSION   DASPYY010000183 DASPYY010000000
VERSION     DASPYY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281169"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2317
                     /locus_tag="VEP73_02760"
     CDS             <1..>2317
                     /locus_tag="VEP73_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25392.1"
                     /translation="TGGRGRLATCEEVDQALTELASPEGLTAGASTFTRAEVVDALAK
                     RLPVAPSAHDALTQAETLAERFLAERSVVIGRDLRLGVERYSTPELLERERELVAAAT
                     QRREEHTGQVRLEVVGSVLDRHATAGADQAAMVEDVCRSGAGVSVVVGRAGSGKTWAL
                     GLAREAFELDGHQVLGAAPTGIAAVGLGEEGFSDIRTVDRLLYDLGHGRAELDAKTVL
                     VVDEAAMLGTRKLAGLLDHASEARAKVVLVGDDRQFASIEAGGGFRALRQRLGASELT
                     VNRRQVAAWEQRAIDDLREGRIEQAIAAYAEHERIRAYDAKDDRDRALIFDWWQAHEA
                     GEQPVILAHRRAQVDQLNQLCQRLRAEHGQLGAERLAVGDRTFAVGDQVVLGANALDR
                     LGVANGTSAEITALDVRARAMTVRVLEEEPPRTVTLPGWYLDAAVRPGQSRRVDLAYA
                     RTDMRSQGRTEQRALLALDGVEDMQGGYVQLTRGKERTDMYLTVGPEPLGPEEAHPHP
                     RGEAVEPEALLGRVLTRDGSKALASDTPALLDVRRMSTGQLREERDRLAELRASCPAD
                     RSRELGLARQRAAELDTARLTAVAEQQAAAAEVAGVGGSVLHRREAAAARERLTLAEH
                     AVRATGRQATQAAERAGMLRRAEAERAGWLERHADLPAQERATARELAWRHRVDARAV
                     ALEQPGWLTAELGPMPAAGEPDGRAAWLAAAVELDTWRRTHGLDGERPAEQGRGERTR
                     PGRAAAPGTARQGRRPTTERVTADDRPAGDQA"
BASE COUNT          301 a          880 c          898 g          238 t
ORIGIN      
        1 caccggcggg cgcgggcggc tcgccacctg cgaggaggtc gaccaggcgc tcacggagct
       61 tgccagccct gaggggctga ccgcgggcgc gtcgacgttc acccgcgcgg aggtggtgga
      121 cgcgctcgcc aagcgcctgc cggtcgcccc gagcgctcac gacgcgctca cccaggcgga
      181 gacgctcgct gagcggttcc ttgctgagcg ctcagtggtg atcggccgcg acctgcggct
      241 gggggtcgag cgctactcca ccccggagct gctcgagcgg gagcgagagc tggtcgccgc
      301 cgccacccag cggcgcgagg agcacaccgg gcaggtccgc ctcgaggtcg tcgggtcggt
      361 ccttgaccgc cacgccaccg ccggggccga ccaggccgcg atggtcgaag acgtgtgccg
      421 ctccggcgcc ggtgtctccg tggtggtcgg tcgggccggc agcgggaaga cctgggcgct
      481 ggggctggcc cgggaggcgt tcgagctgga cggccaccag gtcctcggcg ccgcccccac
      541 cgggatcgcc gccgtcggcc tgggcgagga ggggttcagc gacatccgca ctgtcgaccg
      601 gctgctgtac gacctcggcc acggccgagc agaactcgac gccaagaccg tgctggtggt
      661 cgacgaggcc gccatgctcg gcacccgcaa gctcgccggg ctgctcgacc acgccagcga
      721 ggcgcgggcg aaggtcgtcc tggtcggcga cgaccggcag ttcgcctcca tcgaggcggg
      781 cggcggcttc cgcgccctcc gccagcggct cggcgcaagc gagctgacgg tcaaccgccg
      841 gcaggtggcc gcctgggagc agcgcgccat cgacgacctg cgagaaggcc ggatcgagca
      901 ggcgatcgcc gcctacgccg agcatgagcg catccgcgcc tacgacgcca aagacgaccg
      961 cgaccgcgcc ctcatcttcg actggtggca ggcccacgag gccggcgagc agccagtgat
     1021 cctcgcccac cgccgcgccc aggtcgacca gctcaaccag ctctgccagc ggctgcgcgc
     1081 cgagcacggc cagctcggcg ccgagcggct ggcggtcggc gaccgcacct tcgcggtcgg
     1141 ggatcaggtg gtgcttgggg cgaacgcgct ggaccggctc ggggtcgcca acggcacctc
     1201 cgccgagatc accgccctcg acgtccgcgc gcgggccatg accgtccgtg tcctcgagga
     1261 ggagccgccg cgcacggtca cgctgcccgg ctggtacctg gacgccgcgg tgcggccggg
     1321 gcagtcgcgg cgggtggacc tcgcctacgc ccgcaccgac atgcgctccc agggccgcac
     1381 cgagcagcgc gccctgctcg ccctggacgg ggtcgaggac atgcagggcg gctacgtgca
     1441 gctgacccgc ggcaaggagc gcaccgacat gtacctgacc gtcggccccg agcccctcgg
     1501 ccccgaggag gcccaccccc atccccgcgg tgaggcggtc gagccggagg cgctgcttgg
     1561 gcgggtgctg acccgcgacg gctccaaggc cctcgccagc gacaccccgg ccctgctgga
     1621 cgtgcggcgc atgtccaccg ggcagctccg cgaggaacgc gaccggctcg cggagctgcg
     1681 cgcctcctgt ccggccgacc gctcccggga gctggggctg gcgcggcagc gcgccgccga
     1741 gctggacacc gcccgtctga ccgcagtcgc cgagcagcag gccgccgcag ccgaggtggc
     1801 cggcgtcgga ggcagcgtgc tgcaccgccg ggaggccgcc gcggcgcggg agcggctgac
     1861 cttggcggag catgcggtgc gggccaccgg ccggcaggcc acccaggccg ccgagcgcgc
     1921 cgggatgctg cgccgcgccg aggccgagcg agccggctgg ctggagcggc acgccgacct
     1981 gcccgcccag gagcgcgcca ccgcccggga gctggcctgg cggcatcggg tggacgcccg
     2041 cgcggtcgcg ctcgagcagc caggctggct gaccgccgag ctcggcccga tgccagccgc
     2101 cggggagccg gacgggcggg cggcgtggct ggccgccgcg gtggagctgg acacctggcg
     2161 gcgcacccac ggcctggacg gcgagcggcc cgctgagcag gggcgggggg agcgcactcg
     2221 cccagggcgg gccgctgcgc cgggcacggc ccgccagggc aggaggccga ccaccgagcg
     2281 ggtcaccgcc gacgacaggc ctgcgggtga ccaagcc
//
LOCUS       DASPYY010000184         5284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_174208, whole genome shotgun sequence.
ACCESSION   DASPYY010000184 DASPYY010000000
VERSION     DASPYY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174208"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VEP73_02765"
     CDS             complement(<1..725)
                     /locus_tag="VEP73_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT25393.1"
                     /translation="MQVGEGIHRLTPGVCNFYLVEEGGKLVLVDAGAPRDWDLLVRTL
                     AALGRRLDDLEAVLVTHAHSDHTGFAERARTTAGAPVWIHQADAEVAKGAKPGKNDGR
                     ATSYLLRAEFYRTLFSLTRRGATRMIPIREVSTFADGETLAVPGQPRAVHAPGHTPGS
                     AALLLEGRRVLLSGDTLVTRNPLTGRVGPQVMPSGLNRDTPQALRSLDVFDRVPADVL
                     LPGHGEPWTGGAAEAARLAREAGP"
     gene            complement(806..1906)
                     /locus_tag="VEP73_02770"
     CDS             complement(806..1906)
                     /locus_tag="VEP73_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HYT25394.1"
                     /translation="MAVELQPTQARQGPLARLVALFTPREWARLGGMYGFVALLHLLG
                     WGLFLGVAAKSPGFAGAGVLAYTFGLRHAFDADHISAIDDTTRFMLQKGKRPLGVGLF
                     FSLGHSTVVFGLAFGLAVAAKAVQSRIPEFQHVGGTIGAGVSGVFLWVIGILNLIVLL
                     EIIRIWRRMKQGTYHREHLEELLLQRGLVNRLLGGRAQKFINHSWQLYPLGLLFGLGF
                     DTASEVGLLALTAGVATGKVSFWAVISLPILFAAGMSLMDTTDGALMSKAYGWAFSSP
                     LRKIWYNITTTGLSVAVALLIGTIELLQVLSDRLGWKGAFFDFLNGKLDFGVLGYLIV
                     GMFLLAWLGSVVLWKVRRVEERYGDRLAEAQG"
     gene            2090..2527
                     /locus_tag="VEP73_02775"
     CDS             2090..2527
                     /locus_tag="VEP73_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HYT25395.1"
                     /translation="MVVSGRAGPEGPPPEVVARFAEARVARLATATPGGPRLVPVTFA
                     WHEGALVWAVDSAKPKRTRRLRRLADLEADPRVALLVDHYADDWGELWWVELRGTAAV
                     LEGRAAEAALDALAARYPAYRADRPPGPVVAVTPDGWTWWSAR"
     gene            2707..3534
                     /locus_tag="VEP73_02780"
     CDS             2707..3534
                     /locus_tag="VEP73_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HYT25396.1"
                     /translation="MVSAAIDIGLEQLLAPVPNGEPAFEVRPDDEAAVQRVEGWFRAY
                     ASTRSPALRERIILAYLGLADRLAERYRGNRSIPLEDLRQVARLGLVKAVDRYEPDRA
                     NPFIPYAVATVIGEIKRHLRDASWRIRVPRGTKDLALRLCRAIDELPQRLGHSPTVAE
                     LAEYLKATEEEVLEAIEVAQTRSAPSLDQPAGEDGDAVLGDFLTDTGFREELEDLLVL
                     PQLVGCLPEREREIVLLRYVEELTQDQIAIRMNISQMHVSRLLRRAIERMRAQLVEV"
     gene            complement(3684..>5284)
                     /locus_tag="VEP73_02785"
     CDS             complement(3684..>5284)
                     /locus_tag="VEP73_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25397.1"
                     /translation="LWIAALASNVGYWMQNVGAVWLMTRLSSSAVLVALVQTATSLPV
                     MLVGLPAGALADVVDRRRLLLVTQTWMLASAAALALLTLAGLATPWLVLAFTFLLGLG
                     NAVNAPAWQAVIPELVGPEQTSAAVALNSVQFNIGRALGPALGGLLVAVAGPEAVFAL
                     NALSFLGVIFVLWRWRRPEQDVIGGGERVLGAIGSGLRYLRHAPPLRAVLYRTSLFIL
                     PASALWALLPLVATRELGLGATGYGVLLGGLGAGSIGGAAILPKVRSRFPIDLRLVAG
                     TLLLAGALLVLALVRSVPPVFAAMLAAGLAWVSVLTSFNVATRAAVPRWVQARALAVY
                     LLVFQGGLALGSLVWGLAAARLGDTGALLLAVAALLAGLVGTLRWRLQAIGTFDLSPS
                     VRPEPEVAVEPEPDDGPVLVLVEYRIAPSRAEEFAVAMRALRRVRRRDGAYRWGFWAD
                     VVDPSRYVETFVVRSWAEHLRQHERFTVEDLLVRDRVQSFHVGEEPPAVSHLVHPDAA
                     IGRQRHWVRWTRPGTRGRSASERA"
BASE COUNT          793 a         1890 c         1976 g          625 t
ORIGIN      
        1 ggcccggcct ccctggccag gcgcgcggcc tcggcggcgc ccccggtcca gggctccccg
       61 tggccgggca gcagcacgtc ggccgggacc cggtcgaaga cgtcgagcga ccggagcgcc
      121 tgcggggtgt cgcggttgag gcccgacggc atgacctgcg ggccgactcg gccggtcagc
      181 gggttcctgg tcaccagcgt gtcgccgctc agcagcaccc gccgtccttc cagcagcagg
      241 gcggcgctgc ccggggtgtg gccgggcgcg tgcaccgctc gcggctggcc tggcaccgcc
      301 agcgtctcgc cgtcggcgaa cgtggacacc tcccggatcg ggatcatccg ggtggccccc
      361 ctgcgcgtga gcgagaacag ggtccggtag aactcggccc gcagcaggta cgaggtggcc
      421 ctgccgtcgt tcttcccggg cttggcgccc ttggccacct cggcgtccgc ctggtggatc
      481 cacaccggcg cgccggccgt ggtgcgggcg cgctcggcga agccggtgtg gtcgctgtgg
      541 gcgtgggtga ccagcacggc ctccaggtcg tccagcctgc ggccgagggc cgccagggtg
      601 cgcaccagca ggtcccagtc acggggcgcc ccggcgtcga ccagcacgag cttgcccccc
      661 tcctcgacca gatagaagtt gcagacaccc ggcgtcagcc ggtggatgcc ttccccgacc
      721 tgcacctgac cacctccgcc cacgcgaccc tcgcgtcggg gcaagcatat tgcggagcca
      781 ggggggcgtg cccacctcac ctcggctatc cctgcgcctc cgcgaggcgg tcaccgtagc
      841 gctcctcgac ccggcggacc ttccacagca cgaccgagcc gagccaggcg agcaggaaca
      901 tgccaacgat caggtagccg agcacgccga agtcgagctt gccgttgagg aagtcgaaga
      961 aggcgccctt ccagccgagc ctgtcgctga ggacctgcag cagctcgatg gtgccgatca
     1021 gcagggcgac cgcgaccgac aggccggtgg tggtgatgtt gtaccagatc ttgcgcagtg
     1081 ggctcgagaa cgcccacccg taggccttgc tcatcagcgc gccgtcggtg gtgtccatca
     1141 ggctcatccc ggcggcgaac aggatcggca gggagatcac ggcccagaag gagaccttgc
     1201 cggtggccac gccggcggtg agcgcgagca ggccgacctc gctggcggtg tcgaacccga
     1261 gcccgaacag cagcccgagc gggtagagct gccacgagtg gttgatgaac ttctgggcgc
     1321 gcccgccgag cagccggttg acgaggccgc gctgcagcag cagctcctcg aggtgctcgc
     1381 ggtggtaggt gccctgcttc atccgccgcc agatcctgat gatctcgagc agcacgatca
     1441 ggttgaggat cccgatgacc cacaggaaca ccccggagac gccggcgccg atggtcccgc
     1501 cgacgtgctg gaactcgggg atgcgcgact ggaccgcctt ggccgcgacc gccaggccga
     1561 acgcgagccc gaacacgacg gtggagtgcc cgagggagaa gaacagcccg accccgagcg
     1621 gacgcttgcc cttctggagc atgaaccggg tggtgtcgtc gatcgcggag atgtggtcgg
     1681 cgtcgaaggc gtggcgcagc ccgaaggtgt aggcgagcac cccggcgccg gcgaacccgg
     1741 gggacttcgc ggcgacgccg aggaacaggc cccagccgag caggtgcagc agggcgacga
     1801 acccgtacat gccgccgagc ctggcccact cgcgcggggt gaacagcgcg accagccggg
     1861 ccagcgggcc ctgccgagct tgggtgggct ggagttcgac ggccatgctc gttcctctcc
     1921 ttcggtcacg gtgacccgcc aggcgcaacg acccgccata gttgcacacc actcgcaact
     1981 gagcaatggt tgcaccacca tgcgggtcgc acgggccgcg ggggcgggag atggccccca
     2041 ggcccgtgaa ctggacggct cgagcgagcc aggccgagga tgggtgatcg tggtggtgag
     2101 tggccgggcg ggcccggagg gaccgccgcc cgaggtggtg gcgcggttcg ccgaggccag
     2161 ggtcgcccgg ctggccacgg cgacgcccgg cgggccgagg ctcgtcccgg tgacgttcgc
     2221 ctggcacgag ggcgctctgg tctgggcggt ggacagcgcg aagccgaagc ggaccaggcg
     2281 gctccgccgg ctcgccgacc tggaggcgga cccgcgggtc gccctgctcg tcgaccacta
     2341 cgcagacgac tggggcgagc tgtggtgggt cgagctgcgg ggcacggcag cggtcctcga
     2401 ggggcgggcc gccgaggccg ccctcgacgc gctggccgcc cgctaccccg cctaccgggc
     2461 cgaccggccg cccgggccgg tggtggcggt caccccggac ggctggacgt ggtggtcagc
     2521 ccgctgaatg gggtggtcgg cccgccgacc ggggaccgct cgatcccgcc gtccggccgg
     2581 aaactcgcgg gatgggatcc tgccgccgca ggtttaccct ccgcaggtca aggagaaaac
     2641 gagccaaact gaacagctcg agtccgcctt gtccctgcgg gccggcagta cctcacggag
     2701 gttctgatgg tgtccgcggc gattgacatc ggcctggagc agctcctggc tccggtcccc
     2761 aacggcgagc cggcgttcga ggtccgtccc gacgacgagg ccgcggtcca gcgggtcgaa
     2821 ggctggttcc gggcgtacgc ctcgacccgc agccccgccc tgcgcgagcg gatcatcctg
     2881 gcctacctgg gcctggccga ccggctggcc gagcgctacc ggggcaaccg ctcgatcccg
     2941 ctcgaggatc tgcgccaggt cgcgcggctc ggcctcgtca aggcggtcga ccgctacgag
     3001 ccggaccgtg caaacccgtt catcccctat gcggtggcga ccgtgatcgg cgagatcaag
     3061 cgccacctcc gcgacgcgag ctggcgcatc cgcgtcccgc gcggcaccaa ggacctggcc
     3121 ctgcgcctgt gccgggccat cgacgagctg ccgcagcggc tcgggcactc ccccacggtc
     3181 gccgagctgg ccgagtacct gaaggccacc gaggaggagg tcctcgaggc gatcgaggtg
     3241 gcgcagacgc gctcggcgcc gtccctggac cagccggccg gcgaggacgg cgacgcggtg
     3301 ctcggcgact tcctcaccga cacgggcttc cgggaggagc tggaggatct gctcgtcctc
     3361 ccccagctgg tcggttgcct gcccgagcgg gagcgggaga tcgtgctgct gcgctacgtc
     3421 gaggagctca cccaggacca gatcgcgatc cgcatgaaca tctcgcagat gcacgtgtcg
     3481 cgcctgctcc gccgcgcgat cgagcggatg cgggcgcagc tcgtcgaggt gtgaccggcc
     3541 ctcgtttgga acctcgacgg ggcggcaagg ccctacggga gggccctgac ctcgccgtca
     3601 ccgcctcgac gtgctcgagg ccgcagcggg cccccggcag cggggggccg ctgcgtgccc
     3661 gatgcggttg tggcgtgctc ggttcaggcg cgctcggagg cggaccgtcc acgggtgccc
     3721 gggcgggtcc agcgcaccca gtggcgctgc ctgccgatgg cggcgtcggg gtggaccagg
     3781 tgggagacgg cgggcggctc ctcaccgacg tggaaggact ggacccggtc gcggacgagc
     3841 aggtcctcga cggtgaaccg ctcgtgctgg cgcaggtgct cggcccagga ccggaccacg
     3901 aaggtctcga cgtagcgcga ggggtcgacg acgtcggccc agaaccccca gcggtaggcc
     3961 ccgtcgcggc gccgcacccg ccgcagtgcg cgcatggcga ccgcgaactc ctcggcccgc
     4021 gacggggcga tgcggtactc gaccaggacg aggaccgggc cgtcgtccgg ctccggctcg
     4081 accgccacct ccggctccgg acggaccgag ggcgacaggt cgaaggtccc gatcgcctgc
     4141 agcctccacc tgagcgtgcc cacgaggccg gcgaggagcg cggcgaccgc caggagcagg
     4201 gcgccggtgt ccccgagccg tgccgccgcg agaccccaga cgaggctgcc gagcgcgagc
     4261 ccgccctgga acacgagcag gtagacggcc agggcgcgcg cctgcaccca ccgggggacc
     4321 gccgccctgg tggcgacgtt gaagctcgtg aggaccgaca cccaggcgag gccggcggcc
     4381 agcatggcgg cgaagaccgg cggcaccgag cggaccagcg cgagcacgag cagcgcaccg
     4441 gccaggagca gcgtcccggc gacgagccgc aggtcgatcg ggaaccggct gcggaccttg
     4501 ggcaggatgg cggcgccacc gatcgagccg gcgccgagcc cgccgaggag cacaccgtac
     4561 ccggtcgcgc cgaggccgag ctcccgcgtg gccaccaggg gcaggagcgc ccacagggcg
     4621 ctcgccggca ggatgaacag gctggtccgg tagagcacgg cacgcagcgg cggggcgtgg
     4681 cgcaggtagc gcagccccga cccgatcgcg ccgagcaccc gctcgccacc gccgatgacg
     4741 tcctgctcgg ggcgccgcca gcgccacagc acgaagatga cgccgaggaa cgagagcgcg
     4801 ttgagggcga agaccgcctc cgggccggca accgcgacga ggaggcctcc gagggccggc
     4861 ccgagcgccc ggccgatgtt gaactgcacg ctgttcagcg cgaccgccgc cgacgtctgc
     4921 tcggggccca ccagctcggg gatcaccgcc tgccaggccg gcgcgttgac cgcgttgccg
     4981 agcccgagca ggaacgtgaa cgcgagcacg agccagggcg tcgcaaggcc ggccagggtg
     5041 agcagcgcca gcgcggcggc cgaggcgagc atccaggtct gggtgacgag cagcagccgg
     5101 cgccggtcga ccacgtcggc gagggccccg gcgggcagcc cgaccagcat gaccggcagg
     5161 ctggtggcgg tctgcaccag ggcgacgagc acggcggacg acgacagcct ggtcatgagc
     5221 cacaccgcgc cgacgttctg catccagtag ccgacgttgg aggcgagcgc ggcgatccac
     5281 aggg
//
LOCUS       DASPYY010000185         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1579061, whole genome shotgun sequence.
ACCESSION   DASPYY010000185 DASPYY010000000
VERSION     DASPYY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579061"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..831)
                     /locus_tag="VEP73_02790"
     CDS             complement(205..831)
                     /locus_tag="VEP73_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HYT25398.1"
                     /translation="MLTAVTAGIGLGFLVAAQVGPIWLLCARTVLRGRLASGLAIGAG
                     AAAVDLLYACLGVAGAAQLLRVAALRVTLGLLGAGVLLVLGARTLASAFRVRSGNEVE
                     GDVGTPSAALRTSLVATASNPMTIASWAAVFAAASTASVATSAATTSALLAGIFGGSL
                     AWFTALSLAMAGLRPRLGERGLRAADLLAGAGLVGFGGLLGWRALRST"
     gene            complement(1390..>2028)
                     /locus_tag="VEP73_02795"
     CDS             complement(1390..>2028)
                     /locus_tag="VEP73_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25399.1"
                     /translation="ARDGDGWRWDHDRPRSIGRVHGWNGNFGIIVRAYAYLLANGGDG
                     LRRVAERAVLNANYLLERLTAAYEFGYPTRPVMHEFVLSARAQKAHGARALDLAKALI
                     DRGVHPPTVYFPLIVDEALMVEPTETESMETLDAFAGALLGLAAQAEADAAPLREAPV
                     TAPVARLDEARAARHLVARWHPPPEPEEAGQGPRVASGSDPSDAGPEEKPTS"
BASE COUNT          275 a          742 c          770 g          241 t
ORIGIN      
        1 tcccgttcgg cacgccgagg cgtgcctccg atctccctgt cccgttcggc acgccgaggc
       61 gtgcctccga tctccctgtc ccgttcggca cgccgaggcg tgcctccggg cggcgtgcct
      121 ccgggcggcg tgcctccgcc gtctcccagt atgcaccgtg gcatcggccg cgctccgcga
      181 ggtcgatgga ggatgccggg cggctcaggt ggagcgcagc gcccgccagc cgaggaggcc
      241 accgaagccg accagcccgg cgccggccag caggtcggcg gcccggagac cacgctcgcc
      301 gagccggggc cgcagccccg ccatcgccag cgagagcgcc gtgaaccagg cgaggctgcc
      361 gccgaagatg ccggcgagca gcgccgaggt cgtggccgcc gaggtggcga cgctggcggt
      421 cgaggcggcg gcgaacacgg ccgcccacga ggcgatcgtc atcgggttgg aggcggtggc
      481 gaccagcgag gtgcgcaggg cggcggacgg cgtgcccacg tcgccctcca cctcgttccc
      541 tgaccgcacg cggaacgccg acgcgagcgt gcgggcgccg aggacgagca ggacgccggc
      601 cccgagcagc ccgagcgtga cccgcagcgc ggcgacccgc aggagctggg cggcgccggc
      661 gacgccgagg caggcgtaga gcaggtccac cgcggcggcg cccgcgccga tcgccaggcc
      721 ggacgcgagc cgcccgcgca gcacggtgcg ggcgcagagg agccagatcg gcccgacctg
      781 cgcggcgacg aggaacccga ggccgatccc ggccgtgaca gcggtcagca tgggagccag
      841 tatggcggcg gcacgacccc ccgggcgaca gggtttcgcc ccggccccag gagctgcttc
      901 cacgttccta cgaggagtgc gagattgtcg agcgtgaccg ttatgagcgt gctggatcgg
      961 gagctgtcca cggacccgcg gcaccgtcca cctgcacgcc ggagaggaga tcctccgggt
     1021 cgtgagcggc gagcttgtcg tccgcgtcgg tgaccaacag cggcgctgcc gggaaggaga
     1081 catcgcgatc atcccgccga acacctggca cgggttccat gcccgcaccg acacggtcat
     1141 cgaggtggtc gccgagcagg ccatgggcac cttcttcccg gtgcgccggc cagacgggat
     1201 gcgggccgtc gttgcgacct accggcagga catgaggaga tcgaggccat cctcgccgcc
     1261 gtcgacatcg aggtggaacc ggactggcac ccttgaacgc ccgcgactcg cggcccgggc
     1321 ccaggaacgg cgggctcgaa ccggtgcgcg ccgtcacccg attttcgcgg tggcgtcccc
     1381 ggaccgccgt caggaggtgg gcttctcctc cggcccggcg tcggacgggt ctgacccgga
     1441 cgcgaccctc ggtccctgcc cggcttcctc gggctcgggt ggcgggtgcc agcgggcgac
     1501 gaggtggcgg gcggccctgg cctcgtcgag ccgcgcgacc ggggcggtca cgggcgcctc
     1561 gcggagcggc gcggcgtccg cctcggcctg ggcggcgagc ccgagcaggg cgccggcgaa
     1621 cgcgtccagc gtctccatgc tctccgtctc cgtcggctcg accatgagcg cctcgtcgac
     1681 gatcagcggg aagtacaccg tgggagggtg gaccccgcgg tcgatcagcg ctttggcgag
     1741 gtcgagggcg cgcgcgccgt gcgccttctg ggcccgggcc gacagcacga actcgtgcat
     1801 caccggccgg gtggggtagc cgaactcgta ggcggcagtc aaccgctcga gcaggtagtt
     1861 ggcgttgagc acggcccgct cggccacccg gcggagcccg tcgccgccgt tcgccagcag
     1921 ataggcgtac gcgcgcacga tgatgccgaa gttgccgttc cagccgtgca cgcgcccgat
     1981 cgagcggggc cggtcgtggt cccagcgcca cccgtcgccg tcgcgggc
//
LOCUS       DASPYY010000186         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1018138, whole genome shotgun sequence.
ACCESSION   DASPYY010000186 DASPYY010000000
VERSION     DASPYY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018138"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VEP73_02800"
     CDS             <1..1563
                     /locus_tag="VEP73_02800"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HYT25400.1"
                     /translation="VDVLGPLGRPFAPPGEGPAAGGGPPACLLVGGGYGAAPLHLLAG
                     ELRARGCPVHLVLGASTAGRLLAPEEGTPAGDRLTVTTEDGSAGCRGLATDPLPELLR
                     HAPLAHVYACGPMPMLAAVSRVAAAAGAPCQVAVEERMACGTGICFSCVVPVRGGGPG
                     PGRMARSCTEGPVLDGAGVAWAELGPAPAGRPAAAAAGRTRGQAGAAPGPARAWDPDL
                     SVDLCGVRLATPVLAASGCFGFGREMARSLDLREVGAVVTKSVSLEPRRGRPTPRAAE
                     TPSGMLNAIGLQNPGVEAFCATDLPFLADAGARVVASVVGRTAAEFGRVAGRLAREDA
                     VDLVEVNLSCPNVEHRGEVFAADPAAAAEVVRAVKAAAGQPVLAKLTADVSDLVAVAR
                     AVVEAGADGLTLINTLLGMAVDVHRRRPLLAAGTGGLSGPAIRPVAVRCTWQVASALP
                     GVPIVGTGGVVSAEDAAEFLLVGARAVAVGTASFNDPRAAVAVARGIAAYLETQGARS
                     VAELPALFQPGG"
     gene            1569..>1828
                     /locus_tag="VEP73_02805"
     CDS             1569..>1828
                     /locus_tag="VEP73_02805"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HYT25401.1"
                     /translation="MTSAIVFKEIGFKEKDPGGGRGDSPVVLALDTPDLDLALDWAEL
                     TAPAVGMVKVGLELFCAHGAGAVRALRDEGHRVMLDLKLHDIP"
BASE COUNT          169 a          737 c          696 g          226 t
ORIGIN      
        1 gtcgacgtgc tcgggccgct cgggcgcccg ttcgccccgc ccggcgaggg tcccgccgcc
       61 ggcggcgggc cgccggcctg cctgctcgtc gggggcggct acggtgccgc cccgctccac
      121 ctgctggccg gggagctccg cgcgcgcggc tgcccggtgc acctcgtgct gggcgcgtcg
      181 accgccgggc gcctgctcgc tccggaggag ggcacgcccg ccggcgaccg cctcacggtc
      241 accaccgagg acggctcggc cggttgccgc ggcctcgcca ccgaccccct gcccgagctg
      301 ctccgacacg ccccgctggc acacgtgtac gcctgcgggc cgatgccgat gctcgccgcc
      361 gtgagccggg tcgcggcggc cgcgggggca ccctgccagg tggcggtcga ggagcggatg
      421 gcgtgcggga ccggcatctg cttctcctgc gtcgtccccg tgcggggcgg cggccccggg
      481 ccgggccgca tggccaggtc ctgtaccgag gggccggtgc tcgacggcgc cggcgtcgcc
      541 tgggccgagc tcggcccggc tccggccggg cgccccgccg ctgcggcggc cgggcggacc
      601 cgggggcagg ccggcgcggc ccccggcccc gcccgcgcgt gggatccgga cctctccgtc
      661 gacctgtgcg gcgtccggct ggcgacgccg gtgctcgccg cctccggctg cttcgggttc
      721 ggccgcgaga tggcccgctc cctcgacctg cgcgaggtcg gcgccgtggt caccaagtcg
      781 gtcagcctcg agccccgccg cgggcgcccc acgccccggg cggcggagac gccctcgggg
      841 atgctgaacg cgatcggcct gcagaacccg ggcgtcgagg cgttctgcgc caccgacctg
      901 ccgttcctcg ccgacgcggg tgcgagggtg gtggcgagcg tcgtcggccg caccgccgcg
      961 gagttcgggc gcgtcgccgg gcggctggcc cgcgaggacg ccgtcgacct cgtcgaggtg
     1021 aacctctcat gcccgaacgt cgagcaccgc ggcgaggtgt tcgccgccga ccccgccgcc
     1081 gcggccgagg tggtgcgcgc cgtgaaggcc gccgccgggc agcccgtgct cgccaagctg
     1141 accgccgacg tgagcgacct cgtcgcggtg gcccgggccg tcgtcgaggc gggcgccgac
     1201 ggcctcaccc tgatcaacac cctgctcggc atggcggtcg acgtccaccg ccgccgcccg
     1261 ctgctggccg ccggcaccgg cggcctgtcc ggcccggcga tccggccggt cgccgtgcgc
     1321 tgcacctggc aggtcgcgag cgcactgccc ggggtgccga tcgtcgggac cggcggcgtc
     1381 gtcagcgccg aggacgccgc cgagttcctc ctcgtcgggg cccgcgcggt cgccgtcggg
     1441 accgcgagct tcaacgaccc ccgcgcggcc gtcgcggtcg cccgcgggat cgccgcctac
     1501 ctcgagaccc agggcgcccg ctcggtcgcc gagctgcccg ccctgttcca gccgggaggg
     1561 tgaggagtgt gacctccgcc atcgtgttca aggagatcgg gttcaaggag aaggatcccg
     1621 gcggcgggcg cggagactcg cccgtcgtcc ttgccctcga cactcccgac ctcgacctcg
     1681 ccctcgactg ggccgagctc accgcgccgg cggtcggcat ggtcaaggtg ggcctcgagc
     1741 tgttctgcgc gcacggcgcc ggcgcggtcc gcgcgctgcg ggacgagggc caccgcgtca
     1801 tgctcgacct caagctccac gacatccc
//
LOCUS       DASPYY010000187         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2282126, whole genome shotgun sequence.
ACCESSION   DASPYY010000187 DASPYY010000000
VERSION     DASPYY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282126"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..425)
                     /locus_tag="VEP73_02810"
     CDS             complement(12..425)
                     /locus_tag="VEP73_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25402.1"
                     /translation="MGVSSKGVSSEVEAVALWGDEHDRLGDIATVSLEPCAAIALSRG
                     RFPKAYEHVDLNEDAIMVAGGPAGWLLAVADGHNGFDAARAALGAVALEAGALLEGGG
                     AAPQAKVATLLRLAREAVARDLDGLAQPRAPAARR"
     gene            720..2396
                     /locus_tag="VEP73_02815"
     CDS             720..2396
                     /locus_tag="VEP73_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25403.1"
                     /translation="MENVDQVNSGPGLVAALWRHRALVAAVTALAALAGYGLSLMQPT
                     LYEGQAKLLLSDPRGKSVFQPDQAQPVVLDPSRSIRNRAEVVASRPVVARAATELGRS
                     GQVDLLMQRVSAVPSAELDLITVHALDPTPKGAAALADAVAQAYQSMAAQQVAATARQ
                     TLKQLDRSNRLLKARIAELDQQVKADRENTLRVSERDAAAEQLATGQKRADQIAVEAA
                     LLGSGVDYYEPAKVPTSPAQPHSFGAALLAAILGFLGVGAFAWWRAEHTQVANGRQDA
                     ATVLGTSLLGEVPEFSSVGVTGFTPALTAPKSAAGEAYQFLVASLEFSLSQSSSQSSG
                     RTVLVTSAGPTDGKTVTALNLAVSATRDGRRVLLVDADERARGLTELTGVPPSPGLTD
                     LAGGELPLGACVSDYRLSDDQRLPVVPAGSALDEPAGFFRSPGFRKAIASVKEQADLV
                     VVDSPPLLAVADTSAIASQADGIVVVVSRGTRLRVLEEVRERLEFIGTPVLGYVFNRA
                     DLRRGRASYGRYGRYGRYGRYGYGRRGEAGASSNGDRADGGSRAERRVRQ"
BASE COUNT          325 a          964 c          863 g          314 t
ORIGIN      
        1 cagcgccagg ctcagcgccg tgcggctggg gcgcgcggct gcgccagacc gtccaggtcg
       61 cgcgccacgg cctccctggc caggcggagc agggtcgcga ccttcgcctg gggagccgcc
      121 ccgccgccct cgaggagcgc cccggcctcg agggcgaccg cgccgagcgc ggcccgggcg
      181 gcgtcgaagc cgttgtggcc gtcggcgacc gcgagcagcc atcccgccgg cccgccggcc
      241 accatgatcg cgtcctcgtt gaggtcgacg tgctcgtagg ccttcgggaa gcggccccgg
      301 gacagcgcga tcgccgcgca gggctcgagc gagacggtgg cgatgtcacc cagccggtcg
      361 tgctcgtcgc cccagagcgc gaccgcctcc acctcggacg agaccccctt ggacgagacc
      421 cccatgccgc ttcgccccct tttcgccctt gcggctccaa atgctaatta cccgcgccgg
      481 ctgacgcact ttttgccgca ttgtgccgaa caattcatag ggcaacccgc agcccctcgc
      541 taaatcgtcg cgctcggcgt tccgatgcgt agagcctgtg cacggaccct gtgctaccgt
      601 acgccgatcg gcgcaagggg ggctcggcat gaagccgtga acccgaaaaa accaggcgct
      661 cgcggggcac acgcggcagc ccgagctcga ccgactcgaa ccgaggagcg tccacacgcg
      721 tggaaaacgt cgaccaggtg aattccggcc caggcctcgt cgccgcattg tggcgccacc
      781 gcgcgctcgt cgcggccgtc accgcgctcg ccgcgctcgc cggctacggg ctgtcgctca
      841 tgcagccgac cctctacgag ggccaggcca agctgctctt gtccgatcct cgcggcaaga
      901 gcgtcttcca gccggaccag gcgcagccgg tcgtgctcga cccctcgcgc tccatccgca
      961 accgggccga ggtcgtcgcc tcccggccgg tggtcgcgcg ggccgccacc gagctcggcc
     1021 gcagcggcca ggtcgacctg ctcatgcagc gggtctcggc cgtgccctcg gccgagctgg
     1081 acctcatcac ggtccacgcg ctcgacccga cgccgaaggg cgccgcggcg ctcgccgacg
     1141 cggtcgccca ggcgtaccag tccatggcgg cgcagcaggt ggcggcgacc gcccggcaga
     1201 cgctgaagca gctcgaccgc tcgaacaggc tgctcaaggc ccggatcgca gagcttgacc
     1261 agcaggtcaa ggccgaccgt gagaacacgc tgcgcgtcag cgagcgggac gccgccgccg
     1321 agcagctcgc gaccggccag aagcgggccg accagatcgc cgtcgaggcg gccctgctcg
     1381 gctcgggcgt cgactactac gagccggcga aggtgccgac cagcccggcg cagccccact
     1441 cgttcggcgc cgcgctgctc gcggccatcc tcgggttcct cggcgtcggc gcgttcgcct
     1501 ggtggcgggc ggagcacacc caggtcgcga acggcaggca ggacgctgcc acggtcctcg
     1561 ggacgtccct gctcggcgag gtgccagagt tctcctcggt cggcgtgacc gggttcaccc
     1621 cggccctgac ggcgccgaag tcggcggccg gcgaggccta ccagttcctg gtcgcctccc
     1681 tcgagttctc gctctcccag tcctcctccc agtcctcggg ccggaccgtg ctcgtcacca
     1741 gcgccggccc gaccgacggc aagaccgtga cggcgctgaa cctcgcggtc tcggccacgc
     1801 gggacggccg ccgggtcctc ctggtcgacg ccgacgagcg ggcgcgcggg ctgaccgagc
     1861 tgaccggcgt cccgccgtcc ccgggcctca ccgacctggc cggcggcgag ctgccgctcg
     1921 gggcgtgcgt cagcgactac cgcctctccg acgaccagcg actgccggtg gtcccggccg
     1981 gatccgccct ggacgagccg gccggcttct tccgctcccc cggcttccgc aaggcgatcg
     2041 cctcggtcaa ggagcaggcc gacctggtcg tcgtggactc cccgccgctg ctcgcggtcg
     2101 cggacaccag cgcgatcgcg tcccaggccg acggcatcgt ggtcgtcgtc tcccggggca
     2161 cccgcctgcg cgtcctcgag gaggtgcggg aacggctcga gttcatcggc acgcccgtgc
     2221 tcgggtacgt cttcaaccgc gccgacctgc gccgcgggcg ggcctcctac ggcaggtacg
     2281 gcaggtacgg ccgctacggc aggtacggct acggccggcg aggcgaggcg ggtgcctcgt
     2341 cgaacggcga ccgggcggac gggggctccc gggccgagcg ccgggtccgc cagtgaccga
     2401 ggtcggaggc acgccgcccg gaggcacgcc gcccggaggc acgccgcccg gaggcacgcc
     2461 gcccgg
//
LOCUS       DASPYY010000188         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_736724, whole genome shotgun sequence.
ACCESSION   DASPYY010000188 DASPYY010000000
VERSION     DASPYY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736724"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1285
                     /locus_tag="VEP73_02820"
     CDS             191..1285
                     /locus_tag="VEP73_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYT25404.1"
                     /translation="MGVFDRQTGHEQVVFCQDAATGLQAIVAVYSTALGPSLGGTRFH
                     PYRSEDEALTDALNLSRAMAYKAACAGLDLGGGKGVIVGDPRIDKTEALLESYGRFVQ
                     ALGGRYITACDVGTYVEDMDVVARECAFVTGRSPAAGGAGDSSILTAYGVFQGMRAAA
                     EAVWGSPTLAGRRVGVSGVGKVGRRLVGHLLADGASVVVADVSAEAVGRVRAEHREVE
                     AVDVDALPGLALDVLSPCALGGALDDRTVPALQARVVCGGANNQLAEPGVEKLLEDRG
                     ILYAPDYVVNAGGLIQVAEEIGGYSEARARARATGILDTTRKVLRLAAEEGVPPAVAA
                     DRLAERRIAEASARAGGRILPAGGPRPARP"
     gene            complement(1334..>2230)
                     /locus_tag="VEP73_02825"
     CDS             complement(1334..>2230)
                     /locus_tag="VEP73_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25405.1"
                     /translation="AFLLYLRRFFDPMQELSQFYNSLQSGAAALEKLSGVLDEPPSVP
                     EPRQPVAAGAVRGAVRFDGVRFGYRDRPVLPHLDLDIPAGQTVALVGETGAGKTTLAR
                     LLARFYDPQAGRVTLDGVDLRELADEDLRRAVVIVTQESFLFSGTVADNLAFGRPGAT
                     RAEVEDAARAIGAHDFIAALPDGYDTDVRKRGGRLSAGQRQLVAFARAFLADPAVLIL
                     DEATSSLDVPSERLVQRALRTILADRTAVIIAHRLSTVEIADRVLVVDQGRVVEDGTP
                     AGLLGGAGRYADLRRAWQDSLA"
BASE COUNT          282 a          825 c          859 g          264 t
ORIGIN      
        1 cgtccccgca tcatgtcctc cctccgagcc aggagcaagc taccatcccg gtcgatccag
       61 acaccagcgg ccagccctcc ggtttgcctc cttgcaggga ccgggccgag aattcccggt
      121 cgggccggga cggccggcgc gcgggtccga cgagacgcgg cgggtccgac gagacgcgaa
      181 ggagccagcg gtgggtgtgt tcgaccggca gaccgggcac gagcaggtcg tcttctgcca
      241 ggacgccgca accggcctgc aggcgatcgt cgcggtctac tccaccgcgc tcggaccgtc
      301 cctcggcggc acccgcttcc acccctaccg ctcggaggac gaggccctca ccgacgctct
      361 caacctgtcg cgcgcgatgg cctacaaggc cgcctgcgcc ggcctcgacc tcggcggcgg
      421 caagggcgtc atcgtgggcg acccgcggat cgacaagacc gaggcgctgc tcgagtccta
      481 cggacgcttc gtccaggccc tgggcggccg ctacatcacc gcctgcgacg tcggcaccta
      541 cgtcgaggac atggacgtcg tcgcgcgcga gtgcgcgttc gtcaccggcc gctcgcccgc
      601 cgcgggcggc gcgggggact cgtcgatcct caccgcgtac ggggtgttcc agggcatgcg
      661 tgccgcggcc gaggcggtct gggggtcgcc cacgctcgcg ggccggcggg tcggcgtctc
      721 gggcgtcggc aaggtcggcc gccggctggt cgggcacctg ctcgccgacg gcgcgtccgt
      781 ggtcgtcgcc gacgtgagcg ccgaggcggt cggccgcgtg cgggccgagc accgggaggt
      841 ggaggcggtg gacgtcgacg ccctgcctgg gctcgcgctc gacgtgctct ccccgtgcgc
      901 gctcggcgga gcgctcgacg accgaaccgt cccggcgctg caggcgcggg tggtctgcgg
      961 cggggcgaac aaccagctcg ccgagcctgg cgtggagaag ctgctcgagg accgggggat
     1021 cctctacgcc cccgactacg tcgtgaacgc cggcggcctc atccaggtgg ccgaggagat
     1081 cggcgggtac tcggaggcgc gcgccagggc cagggcgacg ggcatcctcg acacgaccag
     1141 gaaggtgctg cggctcgccg ccgaggaggg cgtgcccccg gcggtcgccg ccgaccggct
     1201 cgccgagcgc cgcatcgccg aggcgagcgc ccgcgccggc gggcggatcc tcccggccgg
     1261 ggggccccgc ccggcccgcc cgtaagggca cggcccgctt gcagggccgc cccgggcggg
     1321 cccgggcggg gcctcaggcg aggctgtcct gccaggcgcg gcgcaggtcg gcgtagcggc
     1381 ccgcgccgcc gagcagcccg gccggggtgc cgtcctcgac gacgcggccc tggtcgacga
     1441 ccaggacccg gtcggcgatc tccacggtgg agagccggtg cgcgatgatc accgcggtcc
     1501 ggtcggccag gatcgtgcgc agtgcccgct ggaccaggcg ctcgctcggc acgtccagcg
     1561 aggaggtggc ctcgtcgagg atcagcaccg ccgggtcggc caggaacgcc cgggcgaacg
     1621 cgacgagctg gcgctggcct gcggacaggc ggccgccgcg cttgcgcacg tcggtgtcgt
     1681 agccgtcggg cagcgcggcg atgaagtcgt gcgcgccgat cgcgcgggcc gcgtcctcca
     1741 cctcggccct ggtggcgccc ggacggccga aggcgaggtt gtcggccacg gtgcccgaga
     1801 acaggaagct ctcctgggtg acgatgacca cggcgcgacg caggtcctcg tccgccagct
     1861 cgcggaggtc cacgccgtcg agcgtcaccc ggccggcctg cgggtcgtag aaccgggcga
     1921 gcagccgcgc cagggtggtc ttgcccgccc cggtctcccc gaccagggcg acggtctggc
     1981 cggccgggat gtcgagatcg aggtgtggca ggaccggccg gtcgcggtag ccgaagcgca
     2041 cgccgtcgaa gcgcacggcg ccgcgcaccg cgccggcggc caccggctgc cgcggctcgg
     2101 gcaccgacgg cggctcgtcc agcacgccgg agagcttctc gagcgccgcg gccccggact
     2161 gcagcgagtt gtagaactgg ctcagctcct gcatcgggtc gaagaagcgc cgcaggtaca
     2221 gcaggaacgc
//
LOCUS       DASPYY010000189         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_315490, whole genome shotgun sequence.
ACCESSION   DASPYY010000189 DASPYY010000000
VERSION     DASPYY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315490"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..143)
                     /locus_tag="VEP73_02830"
     tRNA            complement(68..143)
                     /locus_tag="VEP73_02830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Gly,seq:gcc)
     gene            419..1021
                     /locus_tag="VEP73_02835"
     CDS             419..1021
                     /locus_tag="VEP73_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT25406.1"
                     /translation="MRGAKAYRGLQDGQLVALAAERDAGALEALYDRYGKVSYSLARR
                     ILTDESLAQDVVQEVFLSLWRNAGRFDAGRGTVATYVLSMTHHRAVDVVRREENLRRR
                     RAGDDALELEPDPRPGVEADVLAAERRDEVRAALDALPAGQREALLLAYFGGYTQREV
                     ASLVGVPLGTVKTRMAAGMRKLKDTLSQSGREEQHPWTSR"
     gene            1006..1779
                     /locus_tag="VEP73_02840"
     CDS             1006..1779
                     /locus_tag="VEP73_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYT25407.1"
                     /translation="MDQPMSHAEYEQLAAGYVLGALEPDDEHLFQRHLDGCAVCAAGV
                     RDLEDVAGALAYAAPPVEPPPALRASIRRKVGLTARRRAPLALRRRPSGTLIGGIAVA
                     ACVAALFALAFWNMSLRNQNAVYRQRVAAFEQAGRLLNDPSAQLVRLASPGGGQGGRV
                     TVLASSPHDRGVMVVEDLPAPPAGRVYELWGIPRGSPPSKAVPATVFRSGAGRLALVP
                     FGLSIQPSMTFAVTVEPGPSGSPHPTREPILVGSSTARA"
     gene            1940..2668
                     /locus_tag="VEP73_02845"
     CDS             1940..2668
                     /locus_tag="VEP73_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25408.1"
                     /translation="MTQGQTTAPSLLGLEEDGRDLFGLAAHKLNTPLACIRGFVSTLL
                     QRGNQLDGETVQQFLQLVLTQTDRLQGLVRDFLLLARVNGGVAALARPFRPRDLLLTL
                     IDDLGTEGVRVQPAGDVDAEVVGDAELVRMALRPLVENGLVYGPRLGMVTVTVSVDSS
                     GTRWEVHDGGSRLTSEAVDQLFRPFTRSDEPVERRGSGAGLGLTLARSYAEVLEGSVG
                     GRVDGHGTVFWLEVPTQVYEGAQR"
     gene            2665..3354
                     /locus_tag="VEP73_02850"
     CDS             2665..3354
                     /locus_tag="VEP73_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25409.1"
                     /translation="MTVDAGDRNGSTTRVLVVDDHSVTRHGVVLLCRNAEGVEVIGEA
                     SDGNEAIQRVSELLPDVVLMDVDMPRRDGISATREIRKDHPSVGVVVLTVHEDQETIF
                     EAIKAGASGYLPKSATLDDIRSAVKAVAAGGSFLDPVQARKLLHQFNRYADETKAAAD
                     IYYLLTGREREILALLAEGLTSRQIASQLVISERTVNTHIGNIYRKLHVNNRVDAVRE
                     AMRIRLVEPPR"
     gene            3539..>4375
                     /locus_tag="VEP73_02855"
     CDS             3539..>4375
                     /locus_tag="VEP73_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYT25410.1"
                     /translation="MPAHVLVLDATKGIGPWARDALHDAGLACALVKEEIEAQRSVDA
                     QWPALVVVPIEEIRTWAAVLGRLGEGPTTVPVLGVASRDASIDVTPALAVGVTDLLTH
                     PCTSDDLIVRVTSSMRGRRIPRGPDRVEQGSPLAALRSVTEAAEEALLRTETAAARVS
                     RAEERDHLRLARESLSHSLQVILGTMIGSAEASGPGREGHSRRVASITRELATALGWQ
                     PARVRGIELAALFHDIGLLALPGDLLSVRGPLEEDAIGLLHGHADASADILEPLSRVG
                     VPV"
BASE COUNT          598 a         1588 c         1586 g          603 t
ORIGIN      
        1 cacaccgccg tgccgttctc ggtcgaacgc cccccgggag gtcccagagg gcgtcgagcc
       61 ggaaggatgg agcggatgac gggactcgaa cccgcgaccc tcaccttggc aaggtgatgc
      121 tctaccaact gagccacatc cgcgagaggg ctgcactata gcaacgcagc tcagaccgct
      181 caacagagct tgccacggcg cgctcggggc cgccacgacc ctgcggctcc ggaccgttgc
      241 cccgtctccg acgccggtgc tagcgtccgt cccggatcgt cggcggcctg ccggggtgcc
      301 gccagggccg gcgtccgtac ccgtctgtcg agccggctcg gtgagggcgg gacccgccgg
      361 gcaggcaatc cgacggacgc cgccgcccga aggccatctt ggaaggattc tcacgcgcgt
      421 gcgaggagcc aaggcgtacc ggggtctcca ggacggacag ctggtggcgc tcgccgccga
      481 gcgggacgcc ggtgccctcg aggccctgta cgaccgctac ggcaaggtgt cgtactcgct
      541 cgcgcgccgg atcctgaccg acgagtcgct cgcccaggac gtggtgcagg aggtgttcct
      601 ctcgctgtgg cgcaacgcgg gtcggttcga cgccgggcgg gggacggtgg ccacctacgt
      661 cctctcgatg acccatcacc gggcggtcga cgtcgtccgg cgggaggaga acctgcggcg
      721 gcgccgtgcc ggcgacgacg cgctcgagct cgagcccgac ccacggccgg gggtggaggc
      781 cgacgtgctg gcggccgagc gtcgcgacga ggtgcgagcc gcgctggacg cgcttcccgc
      841 cgggcagcgc gaggcgctcc tcctcgccta ttttgggggg tacacgcagc gcgaggttgc
      901 gagtctggta ggggtccctt tggggactgt gaagacccgg atggcggccg gaatgcgcaa
      961 gctcaaggac accctgagcc agtccggccg tgaggagcag cacccgtgga ccagccgatg
     1021 agccacgccg agtacgagca gctcgccgcg ggctacgtgc tgggcgcgct cgagcccgac
     1081 gacgagcacc tgttccagcg ccacctggac ggctgcgccg tctgcgcggc cggcgtgcgc
     1141 gacctcgagg acgtggccgg ggcgctggcg tacgcggcgc cgccggtgga gcccccgccg
     1201 gcgctgcggg cgtcgatcag gcgcaaggtc ggcctcaccg cccgccggcg cgccccgctc
     1261 gccctgcgga ggcggccgag cgggacgctc atcggcggga tcgcggtggc ggcgtgcgtc
     1321 gccgcgctgt tcgcgctggc gttctggaac atgtcgctgc gcaaccagaa cgccgtctac
     1381 cggcagcgcg tcgccgcgtt cgagcaggca gggcgcctgc tgaacgaccc gtcggcgcag
     1441 ctggtgcggc tcgccagccc gggcggcggc cagggcgggc gggtgaccgt gctggcgtcg
     1501 tcgccgcacg acaggggggt catggtcgtc gaggacctgc cggcgccacc ggccggccgg
     1561 gtgtacgagc tctggggcat ccccaggggc tcgcccccga gcaaggcggt gcccgccacg
     1621 gtcttccggt ccggcgccgg ccggctcgcg ctggtgccgt tcggcctctc gatccagccc
     1681 agcatgacct ttgccgtgac cgtggagccc gggccgtccg gcagcccgca cccgacgcgg
     1741 gagccgattc tggtaggttc ttccacagcg cgggcttgat cgcttcacga ccgacgacga
     1801 ctatgccgga attagtcatc actagcggtt ccccggtgga aagttggaag gcagacttcg
     1861 tctgtatgac aatccctact catcgtgctc ggcggcgtcg gaacgtagtg agcgtgccca
     1921 tgcggaggga aggctgccta tgacccaggg ccagacaacg gctccgtctc tgctgggcct
     1981 cgaggaggac ggcagggacc tcttcgggct cgccgcgcac aagctcaaca cgccgctcgc
     2041 ctgcatccgc gggttcgtgt ccaccctgct gcagcggggg aaccagctcg acggcgagac
     2101 ggtgcagcag ttcctgcagc tcgtgctgac ccagaccgac cgcctgcagg ggctcgtgcg
     2161 ggacttcctg ctgctcgccc gggtcaacgg gggcgtggcg gccctcgcac gcccgttccg
     2221 gccccgcgac ctgctgctga ccctgatcga cgacctcggc acggagggcg tccgggtcca
     2281 gcccgccggg gacgtggacg ccgaggtggt cggcgacgcc gagctggtcc gcatggcgct
     2341 gcggccactg gtggagaacg gcctggtcta cggcccgcgc ctcgggatgg tgacggtgac
     2401 cgtctcggtc gactcgagcg gcacgcgctg ggaggtccac gacggcggct cacggctcac
     2461 cagcgaggcg gtcgaccagc tgttccgccc gttcacccgc tccgacgagc ccgtcgagcg
     2521 ccgcggctcc ggcgccgggc tcggcctcac cctcgcgcgc agctacgccg aggtgctcga
     2581 ggggtcggtg ggcggccgcg tcgacgggca cggtacggtg ttctggctcg aggtacccac
     2641 ccaggtctac gagggagcgc agcggtgacc gtcgacgcag gcgatcgcaa cggctcgacg
     2701 accagggtgc tggtcgtcga cgaccactcg gtgacccgcc acggcgtcgt cctgctgtgc
     2761 cgcaacgccg agggcgtcga ggtgatcggg gaggccagcg acggcaacga ggcgatccag
     2821 cgggtcagcg agctgctccc cgacgtcgtc ctcatggacg tggacatgcc gaggcgggac
     2881 ggcatcagcg cgaccaggga gatccgcaag gaccaccctt cggtgggcgt cgtcgtgctg
     2941 accgtgcacg aggaccagga gaccatcttc gaggccatca aggccggggc gtcggggtac
     3001 ctgcccaagt cggcgacgct cgacgacatt cgctcggcgg tcaaggcggt cgcggccggc
     3061 gggtccttcc tcgacccggt ccaggcccgc aagctgctcc accagttcaa ccgctacgcc
     3121 gacgagacca aggccgccgc cgacatctac tacctgctga ccggccgcga gcgcgagatc
     3181 ctggcgctgc tggccgaggg cctcacgagc cgccagatcg cgagccagct cgtgatctcc
     3241 gagcgcacgg tcaacaccca catcggcaac atctaccgca agctgcacgt caacaaccgg
     3301 gtcgacgccg tgcgcgaggc gatgcgcatc cggctggtcg agccgccccg ctgacgtcgg
     3361 cctgccgcgc ggccggcggt gcttcggttg ctccgcagaa atccgtagcc ccgttcacca
     3421 gttcggtgca tgggacgaac gtggaggaag gccgtagcat cgcgcagcgg acgcttccgg
     3481 ctccggtccc cggagtgacc gcgctcggtg cggtccgcct cgacctggga gcgtctgctt
     3541 gccagcccac gtgctcgtcc tggatgccac gaaggggatc ggcccctggg ctcgtgacgc
     3601 cctccacgac gccgggctcg cctgcgcact ggtcaaagag gagatcgagg cgcagcgcag
     3661 cgtcgacgcc cagtggccgg ccctggtggt cgtgccgatc gaggagatcc gcacgtgggc
     3721 cgctgtgctg gggcgcctcg gcgagggccc caccaccgtc ccggtgctcg gcgtcgccag
     3781 ccgggacgcc tccatcgacg tcacccccgc gctggccgtg ggcgtcaccg acctgttgac
     3841 ccacccgtgc accagcgacg acctgatcgt ccgggtcacg tcctcgatgc gcgggcggcg
     3901 gatcccgcgt gggcccgacc gggtcgagca gggctccccg ctcgccgccc tccgctcggt
     3961 caccgaggcg gccgaggagg ccctgctgcg caccgagacg gcggccgccc gggtcagccg
     4021 cgccgaggag cgcgaccacc ttcgcctcgc gcgcgagtcg ctgtcgcact ccctgcaggt
     4081 catcctcggc acgatgatcg gcagcgccga ggcgagcggc ccgggccgcg aggggcattc
     4141 gcgccgggtc gcctccatca cccgcgagct cgccaccgcg ctcggctggc agccggcacg
     4201 cgtgcgcggg atcgagctgg ccgccctctt ccacgacatc ggcctgctcg ccctcccggg
     4261 cgacctgctc tcggtgcgcg gcccgctcga ggaggacgcg atcgggctgc tgcatgggca
     4321 cgccgacgcc tcggccgaca tcctcgagcc cttgagccgg gtcggcgtcc cggtc
//
LOCUS       DASPYY010000190         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_456904, whole genome shotgun sequence.
ACCESSION   DASPYY010000190 DASPYY010000000
VERSION     DASPYY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456904"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VEP73_02860"
     CDS             <1..254
                     /locus_tag="VEP73_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HYT25411.1"
                     /translation="TFHPTLPRGARVNTEIRGTLKEILVGPAHENDGTLNLFGALRTS
                     MATTGYESVKEFQKAEVMVAPALQTEGKLLQRTQGVGMG"
     gene            complement(337..1179)
                     /locus_tag="VEP73_02865"
     CDS             complement(337..1179)
                     /locus_tag="VEP73_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25412.1"
                     /translation="MRRLPRAHDARLALDEGTAERLLAGRLASADLPPAYARVADLLA
                     AAAAPPDAEELAGEAAAVARFAAAARSRPVRLLHLNPRRSHVLAKLLTVKAAAVALGV
                     LAAGGVAAAATGTLPAPAQQVAHAVLGKAGVPASSAPGASTTTASSASTATTVTTTAA
                     QAHAVGPDAAGPAARGLCRAWAAGQGGQHGKKLDAVAFAALAKAAGGSGKVAAYCAKV
                     AASGAAPSGTAPSSSAVPSTTGGSGRGGSPTSVPPSMVPSSTAPPTTHGGPPSSVPPS
                     TGGH"
     gene            complement(1240..>1516)
                     /locus_tag="VEP73_02870"
     CDS             complement(1240..>1516)
                     /locus_tag="VEP73_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25413.1"
                     /translation="EDESAEPGKTRRRAPGADPEVRSFGAARTCAADGCGTRLSRYNP
                     DSYCSVHRGWDRQVVTRRRRGDEELGAIGDGAIGDLGAAGDLGAATE"
BASE COUNT          172 a          585 c          570 g          189 t
ORIGIN      
        1 ccaccttcca cccgacgctg cctcgcggcg cccgggtcaa cacggagatc cggggcacgc
       61 tcaaggagat cctcgtcggc ccggcccacg agaacgacgg caccctcaat ctcttcggcg
      121 ccctgcgcac gtcgatggcc accaccggct acgagtcggt caaggagttc cagaaggccg
      181 aggtcatggt cgccccggcg ctgcagaccg agggcaagct gctgcagcgg acccagggcg
      241 tgggcatggg ctgagcttgc gttcaccccg gtagatgtac agcggcgcgc caaccgtggc
      301 gcgccgcttc aggctggttc cccgtcctcg acggactcag tgcccgcccg tgctgggcgg
      361 caccgagctt ggcggcccgc catgggtggt gggcggcgcg gtgctggacg gcaccatgct
      421 cggcggcacc gaggtcggcg acccgccacg acccgaaccc ccggtcgtgc tcggcacggc
      481 cgagctggac ggtgcggtgc cagacggcgc cgcgccgctg gcggcgacct tggcgcagta
      541 ggcggcgacc ttgccgctgc ccccggcggc cttggccagc gccgcgaacg cgaccgcgtc
      601 cagcttcttg ccgtgctggc cgccctgccc cgccgcccac gcacggcaca gtccccgcgc
      661 cgccggcccc gcagcgtccg ggcccaccgc atgggcctgg gccgccgtgg tcgtcacggt
      721 cgtggcggtg gacgcgctcg acgcggtcgt ggtcgatgcg cccggcgcgc tcgacgcggg
      781 gacaccggcc ttgccgagca ccgcgtgggc gacctgctgg gcgggcgctg gcagggtgcc
      841 ggtggccgcg gcggcgaccc cgccggcagc cagcacgcca agcgcgaccg ccgccgcctt
      901 gaccgtcaac agcttcgcga gcacgtgcga cctccggggg ttgaggtgca ggagtcgaac
      961 aggacgtgag cgcgccgctg ccgcgaacct cgccaccgcc gccgcctcgc cggccagctc
     1021 ctcggcgtcc gggggggccg ccgcggcggc cagcaggtcg gccacccggg cataggctgg
     1081 cggcaggtcg gcggaagcca gccgtccggc cagcagccgt tcggctgtgc cctcgtccag
     1141 ggccagccgg gcgtcgtgtg ctcgcggcag tcgtcgcatc tcgttcggag agcgtcggcg
     1201 gccagcagag cgttacggcc cttgggccgg cggccgggcc tactcggtgg cggcgccgag
     1261 gtcgcccgcg gcgcctaggt cgccgatggc gccgtcgccg atggcgccga gctcctcgtc
     1321 gccccggcgc cgcctggtca cgacctggcg gtcccaaccc cggtgcaccg agcagtagct
     1381 gtccgggttg tagcgcgaga gccgggtgcc gcagccgtcg gcggcgcagg tccgggcggc
     1441 gccgaaggag cgcacctcgg gatcggcgcc cggggcccgc cgtcgcgtct tcccgggctc
     1501 ggcgctctcg tcctcg
//
LOCUS       DASPYY010000191         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_877427, whole genome shotgun sequence.
ACCESSION   DASPYY010000191 DASPYY010000000
VERSION     DASPYY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877427"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VEP73_02875"
     CDS             <1..868
                     /locus_tag="VEP73_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT25414.1"
                     /translation="DGVHAFLQPDGGWCLNNGGVIVDGDSAVLVDTAATGARARRLRE
                     LVRGVVPAAPRIVVNTHAHGDHTFGNFVFPEAVVVGAELTRAEAVEVGLHLTKLWPDV
                     EWGEVELTPPQVTFTGGLTLHLAGRVAQLHTFGPAHTACDTVVWLPAERVLFTGDLVM
                     SGVTPLVLSGSVGGLRDTIAALRAFGATTIVPGHGAPGGPDLLDATERYLDRVVELAA
                     AGLAEGRAPLDVARGADLGEFAALIDPERLVPNLYRAYAELRGGPSDAAGVDEIFDAM
                     VELHGGLPACHA"
     gene            945..1487
                     /locus_tag="VEP73_02880"
                     /pseudo
     CDS             945..1487
                     /locus_tag="VEP73_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
     gene            1603..2652
                     /locus_tag="VEP73_02885"
     CDS             1603..2652
                     /locus_tag="VEP73_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25415.1"
                     /translation="MRRVGIRQFSTNHLMGDGYWVWLIPLGSDHISIGVCADPRISPY
                     EEINDFDRLLDWLRRHEPQLAEIVETRGDDVADFLTVEDFAFGIERVYSPDRWTLVGE
                     AGAFADAFYSPGSDMIGYGNSVTTDLVVRDLAGEDVTERLEFYNDFHLKTFAFVLSKI
                     TDQYLAFGNPVVMVPKLCWDAMLNHVGVVLLFVKNRFLDYEFLKRVRPDIERLYALND
                     RVQQIFRDWNVLERVETEGPANPYVAAKAIRDSHGQLVVDYSDDELAAAIERNVRVAE
                     ALAVGIFHRAAQGLAERSDPAVPMNPYAVGLQPDRWEADGLFAGDVLLTLEQAEEISE
                     GMQGLFVDRLLAGAS"
     gene            2649..3155
                     /locus_tag="VEP73_02890"
     CDS             2649..3155
                     /locus_tag="VEP73_02890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYT25416.1"
                     /translation="MTEAALVDTRLLRSVCGHFTTGVTVVTSGSTSFVVGVTVNSFTS
                     VSLEPPLILVCIHNDSSDLPALRGTGAFAVNILAADQEEVCRTFARRHTRRATGVDVH
                     PGLTGVPILSKALAYLECRLRREVDGGDHAILIGEVVALDVLRDDRPLAFFRSAFHHL
                     PHDLPNGS"
     gene            3175..4323
                     /locus_tag="VEP73_02895"
     CDS             3175..4323
                     /locus_tag="VEP73_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HYT25417.1"
                     /translation="MTGLSAAAAGIAVAAGRRAEAAERRREVDPAVMAAVVEAGFARH
                     FAPAALGGREGSFAELVDAVGVVAGECPATAWCASLIAGIARMAGAYLPTDGQAEVWA
                     SGPDAVLVGSVTPFGSAEPRPGGWLLSGRWPYMSAVAYSDWAIVCGVAQSGTATEGWL
                     FAVPRSAYRVEDTWFSVGMAATGSNTLVAADVFVPEHRAFKRAELIEGRLDDFTGGAP
                     ACYRAPLPAVNGLMFAAPAVGAAQGMARTFAAAFADKLGSGQLPGTVGVQGVRASAEL
                     ALARSAGEADAARLLLDRAAGLADRCANPGGAMSRLDAARNLRDCSLATDLAVTAVDR
                     LFRQAGTRGQATSGPMQRLWRDVTAVSTHVALQFEPAARGYVEQLVKQ"
     gene            complement(4328..>4729)
                     /locus_tag="VEP73_02900"
     CDS             complement(4328..>4729)
                     /locus_tag="VEP73_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT25418.1"
                     /translation="GGMLQRGSGRIVNIASTGGKQGVINASPYTASKHGVVGFSKSLG
                     LELAKTGITVNAVCPGFVETPMAERVREHYAEIWGVDEDEAHGRITARVPIGRYVEPK
                     EVAAMVTYLVGDGAAAVTAQALNVCGGLGNY"
BASE COUNT          613 a         1731 c         1716 g          669 t
ORIGIN      
        1 cgacggcgtc cacgcgttcc tgcaacccga cggcggatgg tgcctcaaca acggcggcgt
       61 gatcgtcgac ggcgactcgg cggtgctcgt cgacaccgcc gccaccgggg cgcgggccag
      121 gcggctgcgc gagctggtcc ggggcgtggt gccggccgcg ccccgaattg tcgtcaacac
      181 ccacgcgcac ggcgaccaca ccttcggcaa cttcgtcttc cccgaggcgg tcgtcgtcgg
      241 cgccgagctg accagggccg aggcggtcga ggtcgggctg cacctgacga agctgtggcc
      301 ggacgtggag tggggcgagg tcgagctgac gccgccgcag gtgaccttca ccggcggcct
      361 gacgctgcac ctcgccggcc gcgtggcgca gctgcacacg ttcggcccgg cgcacaccgc
      421 ctgcgacacc gtcgtgtggc tgccggccga gcgcgtgctg ttcaccggcg acctggtgat
      481 gtcgggcgtg acgccgctcg tgctgagcgg ctcggtcggc gggctgcgcg acacgatcgc
      541 cgcgctgcgc gcgttcggcg ccaccacgat cgtgccgggg cacggcgcgc ccggcgggcc
      601 ggacctgctg gacgccaccg agcgctacct ggaccgggtc gtggagctgg ccgcggccgg
      661 cctggccgag gggcgcgccc cgctggacgt ggcccgtggc gccgacctcg gcgagttcgc
      721 cgcgctcatc gaccccgagc ggctcgtgcc caacctctac cgcgcctacg ccgagctgcg
      781 cggcgggccc tcggacgcgg ccggcgtcga cgagatcttc gacgcgatgg tcgagctgca
      841 cggcggcctg cccgcctgcc acgcctgagg gcgtcaaccc ggccttgagc ggcccgcgag
      901 cgagcgccag cagactgggc ccgtcgcgaa cagtaggagg aatgatgacc gatcagcgta
      961 ggcacgacgt ggtgatcctc ggcggcgggc tcgcgggcct cacactcgcc atccagctca
     1021 agaccgcgcg tcccgagacc agcgtcgtgg tgctcgacaa gcgcgagggc ctggccccgg
     1081 aggcggcgtt caaggtgggt gagtcgacgg tcccggcggg cgcgcactac ttcgccgagg
     1141 tgctgggcat gaaggagcac ctggacaagt tccatctgaa gaagaacggt ctgcgcttct
     1201 tccagcccgc cggtgacaac accgacctga cgcggcgcat cgagctcggg ccgccggact
     1261 tcccggcgca cgacaactac cagatcgacc gcggccggtt cgagaacgag ctcacccggc
     1321 tggcgctgag cctcggcgcc gacctgcggc agggctgctc cgtgcgggac gtgacgctgc
     1381 ggccggaggc cgaccacgtc gtgtcctaca cgcagggcgg cgcggagcgc accgtggagg
     1441 cccggtggct ggtcgacgcc gccggccgcg cccacgtgct gcggcgcaag ctcgggatcg
     1501 gccacgaggt cccgcacacc gtcaacgcgt cctggttccg cctcaccggc ggcatcgacc
     1561 tggagcagtt ggggtgccga caacccggcc tggctcggcc ggatgcgccg ggtcggcatc
     1621 cgccagttca gcaccaacca cctcatgggc gacggttact gggtctggct gatcccgctc
     1681 ggctccgacc acatcagcat cggcgtctgc gccgacccgc ggatcagccc gtacgaggag
     1741 atcaacgact tcgaccggct gctcgactgg ctgcgccggc acgagcccca gctggccgag
     1801 atcgtcgaga cgcgtggcga cgacgtcgcg gacttcctca ccgtggagga cttcgccttc
     1861 ggcatcgagc gggtgtactc cccggaccgc tggaccctgg tcggcgaggc gggcgcgttc
     1921 gccgacgcgt tctactcccc cggctctgac atgatcggct acggcaacag cgtcacgacc
     1981 gacctggtcg tgcgggacct ggccggcgag gacgtcaccg agcggctcga gttctacaac
     2041 gacttccacc tcaagacgtt cgccttcgtg ctgtcgaaga tcaccgatca atacctcgcg
     2101 ttcggcaacc cggtcgtgat ggtgccgaag ctgtgctggg acgcgatgct caaccacgtc
     2161 ggcgtggtgc tgctgttcgt caagaaccgg ttcctggact acgagttcct caagcgggtc
     2221 cgccccgaca tcgagcggct ctacgccctc aacgaccggg tgcagcagat cttccgcgac
     2281 tggaacgtcc tggagcgggt cgagacggag ggcccggcga acccctacgt cgcggccaag
     2341 gcgatccgcg acagccacgg gcagctggtc gtcgactact ccgacgacga gctggccgcc
     2401 gccatcgagc gcaacgtccg cgtcgccgag gcgctcgcgg tcggcatctt ccaccgggcc
     2461 gcgcagggcc tcgccgagcg gtctgacccg gccgtgccga tgaacccgta cgcggtcggc
     2521 ctgcagcctg accggtggga ggccgacgga ctgttcgccg gtgacgtgct gctcacgctg
     2581 gagcaggccg aggagatctc ggagggcatg caaggcctgt tcgtcgaccg cctgctcgcg
     2641 ggtgcgtcat gaccgaggcc gccctcgtcg acacccggct gctgcgcagt gtctgcggcc
     2701 acttcacgac cggggtcacg gtggtcacca gcggaagcac ctccttcgtc gtcggcgtga
     2761 ccgtgaactc gttcacgtcg gtgtcgctgg agccgccgct gatcctggtg tgtatccaca
     2821 acgactccag cgacctgccg gcgctgcggg gcacgggcgc gttcgcggtc aacatcctcg
     2881 ccgccgacca ggaggaggtg tgccggacct tcgccaggcg gcacacccgc cgggccaccg
     2941 gcgtcgacgt ccatcccggc ctcaccggcg tgccgatcct gagcaaggcg ctggcatacc
     3001 tcgagtgccg cctgcgccgc gaggtggacg gcggtgacca cgcgatcctc atcggcgagg
     3061 tggtggccct ggacgtgctg cgcgacgacc gcccgctcgc gttcttccgt agcgccttcc
     3121 accacctgcc gcacgacctg ccgaacggct cttgaactca cgagggggaa ccgcatgacc
     3181 gggttgtccg ccgccgcggc cgggatcgcg gtggccgccg ggcgccgcgc ggaggccgct
     3241 gagcgccgcc gggaggtcga ccccgccgtc atggcggccg tcgtggaggc gggcttcgcc
     3301 cggcacttcg cgccggccgc cctcggtggg cgggagggct ccttcgcgga gctggtcgac
     3361 gcggtcggcg tggtcgccgg cgagtgcccg gcgaccgcct ggtgcgcctc gctcatcgcc
     3421 ggcatcgccc ggatggccgg ggcctacctc ccgaccgacg ggcaggccga ggtgtgggcc
     3481 tcgggccccg acgcggtgct cgtcgggtcc gtgacgccgt tcggcagcgc cgagccgcgc
     3541 cccggcggct ggctgctctc cggccgctgg ccgtacatga gcgcggtcgc ctactcggac
     3601 tgggcgatcg tgtgcggcgt cgcgcagtcc ggcaccgcga ccgaggggtg gctcttcgcg
     3661 gtgccccgct cggcgtaccg cgtcgaggac acctggttca gcgtcggcat ggcggccacc
     3721 ggcagcaaca cgctggtggc cgcggacgtc ttcgtgcccg agcatcgggc gttcaagcgg
     3781 gcggagctga tcgaggggcg gctggacgac ttcaccggcg gcgcgcccgc gtgctaccgg
     3841 gcgccgctgc cggcggtgaa cggcctcatg ttcgccgccc cggcggtcgg cgcggcgcag
     3901 ggcatggcgc ggaccttcgc ggccgcgttc gccgacaagc tcggcagcgg gcagctgccc
     3961 ggcacggtcg gcgtgcaggg cgtgcgcgcc tccgcggagc tcgccctggc ccgctcggcc
     4021 ggcgaggccg acgccgcgcg gctgctgctg gaccgggcgg ccgggctggc cgaccggtgc
     4081 gccaacccgg gcggggcgat gagccggctg gacgcggccc ggaacctgcg cgactgctcc
     4141 ctcgcgaccg acctggcggt gacggccgtg gaccggctgt tccgccaggc cgggacgcgg
     4201 gggcaggcga cgtccggccc gatgcagcgc ctgtggcggg acgttaccgc cgtctccacc
     4261 cacgtggcgc tgcagttcga gccggccgcc cgcggctacg tcgagcagct cgtcaagcag
     4321 tgactgctca gtagttcccg agcccgccgc agacgttcag cgcctgcgcc gtgacggccg
     4381 cggccccgtc gccgacgagg taggtgacca tggccgcgac ctccttcggc tccacgtacc
     4441 gcccgatcgg cacccgcgcg gtgatccggc cgtgtgcctc gtcctcgtcg acgccccaga
     4501 tctcggcgta gtgctcccgc acccgctccg ccatcggggt ctcgacgaag ccggggcaca
     4561 cggcgttgac ggtgatgccc gtcttggcca gctccagccc gagcgacttg ctgaagccga
     4621 cgacgccgtg cttcgaggcg gtgtagggcg acgcgttgat gacgccctgc ttgccgccgg
     4681 tcgaggcgat gttgacgatg cgcccggagc cgcgctgcag catgccgcc
//
LOCUS       DASPYY010000192         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1721155, whole genome shotgun sequence.
ACCESSION   DASPYY010000192 DASPYY010000000
VERSION     DASPYY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721155"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1709
                     /locus_tag="VEP73_02905"
     CDS             <1..>1709
                     /locus_tag="VEP73_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25419.1"
                     /translation="RLGHLLGLVAAPPGDREELFGAWRRYVERIADRGPTVLVFEDLH
                     WADPGLLDFIESLLEWSRTSPVLVVALARPELAERRPSWGTALRNVTGLHLDPLPDPA
                     VAQLVTGYVHGLPDDGLARLVQRAEGVPLYAVETVRMLADRGVLEQSGEVYRVVADLS
                     GQLEIPETLHALVAARLDGLPDEDRALLQDAAVVGQHFTVAGLAAVSGRTVDELEPRL
                     RVLTRKELLAQDVDPRSPERGQYGFVQAVIREVAYSTLSKPVRRAKHLAAARYYESLD
                     DIELCGIVASHFLEAYRAEPGASGAEDVATQARVWLACAAERAQSLGAPEQALSYAEQ
                     ALPLAADVADRAEILRLAALAALFSGDMDRSVVLARDAVAALRSAGDADGLGRFVAVA
                     VRGLASSRSVDVADMLGAAEEVMADRRGPAAAMVAAVIADHASSAARRDRAMLWSEKA
                     MQLAEETGDFEALRAAASSRSWSLFNSGRHWEAVLLARGVVELAERSGSTYELGRAQM
                     GLGVVLGEDDPHGSMQAFLDAAETATQAGVRPLRWMNLANAAEAAADVGAWDVADRAL
                     AET"
BASE COUNT          209 a          591 c          663 g          246 t
ORIGIN      
        1 cacggctcgg gcacctgctc gggctggtgg ccgcgccgcc gggggaccgt gaggagctgt
       61 tcggggcgtg gcggcggtac gtcgagcgga tcgcggaccg cggtccgacg gtgctcgtct
      121 tcgaggacct gcattgggcc gaccccgggc tgctcgactt catcgagtcg ctgctggagt
      181 ggtctcgcac ctcaccggtg ctggtcgtcg cactcgcccg tcccgagctc gccgagcggc
      241 ggccgagctg gggcaccgcc ctgcgcaacg tcacaggtct gcacctggac ccgctgccgg
      301 acccggccgt cgcgcagctg gtcacgggct acgtgcacgg tctgcccgac gacggcctgg
      361 cccggctggt gcaacgggcg gagggcgttc cgctgtatgc cgtggagacc gtgcgcatgc
      421 tcgccgaccg cggcgtcctc gagcagagcg gggaggtgta ccgcgtcgtc gcggacctga
      481 gcggccagct ggagatcccg gagacgctgc acgcgctggt cgcggcacgg ctggacggcc
      541 tgcccgacga ggaccgcgcg ctgctccagg acgctgcggt cgtcgggcag cacttcacgg
      601 tcgcgggcct ggctgcagtc agcggtcgaa cggtcgacga gctggagccg aggctacggg
      661 tgctgacgcg caaggagctc ctggcgcagg acgtcgatcc gcgctcgccc gagcgcgggc
      721 agtacggctt cgtgcaggcc gtcatccggg aggtcgcgta ctcgaccctg tccaagccgg
      781 tccggcgggc caagcacctg gcggctgctc gttactacga gagcctggac gacatcgagc
      841 tgtgcgggat cgtggccagc cacttcctgg aggcctaccg ggccgagccc ggtgcttccg
      901 gtgccgagga cgtcgcgacg caggcccgcg tgtggttggc atgcgcggcc gagcgcgcgc
      961 agtctctggg ggcgccggag caggccctct cgtacgccga gcaggccctg ccgctggcag
     1021 ccgacgtcgc cgaccgggcc gagatcctgc ggctggccgc gttggcggct ctgttcagcg
     1081 gcgacatgga tcgaagcgtc gtcctcgccc gcgacgctgt ggcggcgctg cgttcggccg
     1141 gtgacgccga cggcctcggg cggttcgtgg cagtggctgt ccgaggcctc gccagctccc
     1201 gctcggtgga cgtcgccgac atgctcggcg cggccgagga ggtgatggcg gatcggcgtg
     1261 gcccggcggc ggcgatggtc gccgccgtca tcgccgatca cgcgtcgtcc gcggcgcggc
     1321 gtgaccgggc gatgctctgg tccgagaagg cgatgcagct cgccgaggag accggtgact
     1381 tcgaggcgct gcgtgctgcc gccagctcgc gcagctggtc gctgttcaac agcggcaggc
     1441 attgggaggc ggtgcttctc gcccgaggtg tggtcgagct ggccgaacgg tcgggcagca
     1501 cctacgagct cggccgcgcc cagatggggc tcggagtcgt gctcggcgag gacgacccgc
     1561 acggcagcat gcaggcattc ctcgacgcgg ccgagacggc cacccaggcc ggagtccgac
     1621 cgctgcgctg gatgaacctg gccaacgccg ccgaggcggc tgccgacgtc ggcgcctggg
     1681 acgtcgccga ccgggcgctc gccgagacc
//
LOCUS       DASPYY010000193         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2283654, whole genome shotgun sequence.
ACCESSION   DASPYY010000193 DASPYY010000000
VERSION     DASPYY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283654"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VEP73_02910"
     CDS             complement(<1..826)
                     /locus_tag="VEP73_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYT25420.1"
                     /translation="MDHVAQAPVAVVEVDDRQLLKLLHWYDGFVVCLANPGFLIASLG
                     ASIGSLGAWGAFALWTLSMVIAVVQNKVYTEPALMFPDRAGGIPIYAHEGWRRYFNFV
                     GPMAAVGYWAGWTVVLAINGLTIGSLVQAQWFPKSTWTGNVLGVSLTFPKVLAIVLIV
                     VVWAINASGVRPAVWVGYLTGALLLIPLALLMFFPYITGNFHSANLTWLIGGAGSTWD
                     SWRLALVWLYLMAWSAYGVEAAATFAPEYRDSERDLTLALRRSAMFSLVVYALLPLG"
     gene            complement(1160..1687)
                     /locus_tag="VEP73_02915"
     CDS             complement(1160..1687)
                     /locus_tag="VEP73_02915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYT25421.1"
                     /translation="MVDRSGAPPDPEPVEPAGGQISVEDFKRAAGSFITGVTVVTTRA
                     PDGKLMGFTANSFTSVSLDPPLVLICLHRNAPSLHAFQSGGGFSVNVLGADQEWVSRH
                     FSRPSEDKFASVAYRPGTTGAPLIEGCIAHIDCSLEVSYDGGDHVILLGRVRSVATGD
                     GSPLVFHRSRYLRAV"
     gene            1801..2628
                     /locus_tag="VEP73_02920"
     CDS             1801..2628
                     /locus_tag="VEP73_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT25422.1"
                     /translation="MPRTGKPATRTVQSVDRGLALLWMLVDAPTGVGVVEASERLGVD
                     PSTASRLLASLVAADLAARDAVTGRYRVGPGAARLAGAAPMAATAGERELRQAARGTL
                     YLLEEGTGETVMLDVRGQGTATVVDVVPSRRSAPHLAWLGTSRPLHATADGKVLLAHA
                     DDGVVDAELGGGLERFTPRTVAEPEALRSQLAQVRRLGYAVTEGERQPGVNVIAAPVE
                     DATGGVAAALAIEGPAERFDRIGMRRALDLLLPRAGELSRNLGSRTGSGAPLPDPFA"
     gene            complement(2633..>3218)
                     /locus_tag="VEP73_02925"
     CDS             complement(2633..>3218)
                     /locus_tag="VEP73_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT25423.1"
                     /translation="FVHPFDDPEIVAGQGTAALELVADAGPVDVWVCCVGGGGLLCGT
                     ALAVRDANPGCRVVGVEPEGAAAVTAALAEGHPVPIVPRSVADGLCAPFAGPRTLPLF
                     QRVVDEVVLVTDEEILAAVRFVMERMKVVVEPAGAAAVAALLAGRAGDVAGARVGTIL
                     SGGNLDLGAVVPQLPPGLARLRQPRPARLRQPRP"
BASE COUNT          485 a         1137 c         1199 g          397 t
ORIGIN      
        1 cgccgagcgg cagcagcgcg tagacgacca gcgagaacat cgccgaccgg cgcagggcga
       61 gggtcaggtc ccgctcgctg tcccggtact cgggcgcgaa cgtggcggcc gcctcgacgc
      121 cataggccga ccaggccatc aggtacagcc acaccagcgc gagccgccag ctgtcccacg
      181 tggagccggc gccgccgatc agccaggtga ggttcgcgct gtggaagttg cccgtgatgt
      241 aggggaagaa catcagcagg gcgagcggga tgagcagcag tgcgccggtc aggtagccga
      301 cccagacggc gggcctgacc ccggaggcgt tgatcgccca gacgaccacg atcagcacga
      361 tcgcgaggac cttggggaac gtgagcgaca cgccgaggac gttgcccgtc caggtcgact
      421 tcgggaacca ctgcgcctgg acgagcgagc cgatcgtgag gccgttgatg gcgagcacga
      481 ccgtccagcc ggcccagtag ccgaccgcgg ccatcggccc cacgaagttg aagtagcggc
      541 gccacccctc gtgggcgtag atggggatgc cgccggcgcg gtcggggaac atcagcgccg
      601 gctcggtgta gaccttgttc tgcacgaccg cgatgaccat cgacagggtc cacagggcga
      661 aggctcccca tgcgcccagc gagccgatcg aggcgccgag gctggcgatg aggaagccgg
      721 ggttggcgag gcagacgacg aagccgtcgt accagtggag cagcttcagc aactggcggt
      781 cgtcgacctc gacgaccgca accggtgctt gggcgacatg atccataggt gcttctcccc
      841 cgggggttaa ggaagatcga ctgctttggc caggctgacg cgcgccacgg gcacgggccc
      901 tgccggaccg ggccggggtc gggtgcgcgg cgacctcgga cgtcggcagc ggtcatgcgt
      961 ggcgcgggct tcaggggtca gggaggaggg gcgcgggtgg cctggtcggc caatgtcgct
     1021 cctctcgctc cggtggcaga tgccacgtcc atccgatcgg gtgaacgggc aataccataa
     1081 cgcctacgcc agagggcact tagcagctgc tatctagtaa ctgttaccta gtgccacttc
     1141 tccgttgcgg gaggcagggt cagacggccc gcaggtagcg gctacggtgg aagacgagcg
     1201 gggacccgtc gcccgtggcc acgctccgca ccctgccgag caggatcacg tggtcaccac
     1261 cgtcgtagga gacctcgagc gagcagtcga tgtgtgcgat gcacccttcg atcaggggcg
     1321 cgccggtggt gccgggccgg taggcgaccg acgcgaactt gtcctccgag ggccgcgaga
     1381 agtggcggga gacccactcc tggtcggcgc cgaggacgtt gacggagaag ccgcccccgc
     1441 tctggaacgc gtgcaggctg ggcgcgttcc ggtgcaggca gatgagcacg aggggtgggt
     1501 cgagcgacac cgacgtgaac gagttggccg tgaagcccat gagcttgccg tcgggtgccc
     1561 tggtggtcac cacggtgacg ccggtgatga acgagccggc ggcacgcttg aagtcctcga
     1621 cgctgatctg cccgccggcc ggctcgaccg gctcgggatc cggcggcgcg ccgctacggt
     1681 cgaccatccg gcccatgtcc tcctgagcga gcaacccgac cacctctgct attattgcca
     1741 aggagccaac agttattggg gctcgaccaa gagcgtagcg tgtcacgcag gaggaaacaa
     1801 gtgccgcgta ccgggaaacc cgccacccgg accgtgcagt ccgtcgaccg cggcctcgcc
     1861 ctgctctgga tgctggtcga cgccccgacc ggggtcggcg tggtggaggc gtccgagcgc
     1921 cttggcgtcg acccgagcac ggcctcccgg ctcctcgcct ccctcgtcgc cgccgacctc
     1981 gcggcccgcg acgcggtgac cggccggtac cgggtgggcc ccggcgccgc ccggctggcc
     2041 ggcgccgccc ccatggcggc caccgccggc gagcgcgagc tgcgccaggc cgcccgcggg
     2101 accctctacc tgctcgagga ggggaccggc gagacggtca tgctggacgt gcggggccag
     2161 gggacggcca ccgtcgtcga cgtggtgccg tcgaggcggt cggcgcccca cctcgcctgg
     2221 ctcggcacga gccggccgct gcacgccacg gccgacggca aggtgctgct cgcccacgcc
     2281 gacgacggcg tcgttgacgc cgagctggga ggcggcctcg agcgcttcac gccgcggacg
     2341 gtcgccgagc cggaggcgct ccggtcgcag ctcgcgcagg tccgccggct cggctacgcg
     2401 gtcaccgagg gcgagcgcca gcccggcgtc aacgtgatcg ccgcgccggt cgaggacgcg
     2461 acgggcgggg tggcggccgc cctcgcgatc gaggggccag ccgagcgctt cgaccgcatc
     2521 ggcatgcgcc gggcgctcga cctcctgctg ccgcgggccg gcgagctgtc ccgcaacctc
     2581 gggtcgcgca ccggctcggg agccccgctg cccgacccct tcgcctgagg gactacgggc
     2641 gtggttgcct gaggcgcgcg gggcgtggtt gcctgaggcg cgcgaggccc ggggggagct
     2701 ggggcacgac cgcgccgagg tccaggttcc cgccggacag gatcgtcccc acccgcgcgc
     2761 ccgccacgtc gccggccctg ccggcgagca gggcggcgac ggccgcggcg ccggccggct
     2821 ccacgacgac cttcatgcgc tccatcacga accgcaccgc ggccaggatc tcctcgtcgg
     2881 tcacgagcac gacctcgtcc acgacccgct ggaacagggg cagggtgcga ggcccggcga
     2941 acggggcgca gagcccgtcg gcgaccgagc ggggcacgat cggcaccggg tggccctcgg
     3001 cgagggcggc ggtgaccgcg gcggccccct ccggctcgac cccgaccacc cggcagccgg
     3061 ggttggcgtc gcggacggcc agcgccgtgc cgcagagaag gccgccccca ccgacgcagc
     3121 agacccagac gtcgaccggc ccggcgtcgg ccaccagctc gagcgctgcc gtgccctggc
     3181 cggccacgat ctcggggtcg tcgaacgggt ggacgaag
//
LOCUS       DASPYY010000194         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1441576, whole genome shotgun sequence.
ACCESSION   DASPYY010000194 DASPYY010000000
VERSION     DASPYY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441576"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VEP73_02930"
     CDS             complement(<1..852)
                     /locus_tag="VEP73_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYT25424.1"
                     /translation="MTRHVALDGGPAGKPVAAGGAVLVGQLELAEPICDLTVPARDGL
                     SYSATRLLVRLHGQPLGFMTVPLVDGWPDPVRVARLLWEELGAEVNAHLTEDGLGRVT
                     SIPVAGLGAERRPRCLQAPAGGPGHPTVTVVVCTRDRPQSLVRCLDAVAAVHYDAFEV
                     IVVDNAPTTRETLEVVRERSGTGSRVRYVREPRPGLSCARNRGLAEARGELVAFTDDD
                     VIVDPRWLDGVVRGFGRSPSVACVTGLVPSARLENAEQRYFDQRVSWAVSCAPRRYDR
                     HTGTRESP"
     gene            complement(849..1217)
                     /locus_tag="VEP73_02935"
     CDS             complement(849..1217)
                     /locus_tag="VEP73_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25425.1"
                     /translation="MNHQESHRQTWMAVFDVSLLGVTALLALLVVLDVDGLPRVLTAL
                     AFACLVPGGALVTRLPFREPDEVLAMTVVLSLAICAAASLLMAWLGAWHPLRLMAGLA
                     AVSATVLVLDLVSTPADDPR"
     gene            complement(1220..>2009)
                     /locus_tag="VEP73_02940"
     CDS             complement(1220..>2009)
                     /locus_tag="VEP73_02940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT25426.1"
                     /translation="QDWPFGLRYLRAARPGLALAHNEALSAVTGEIVAFTDDDVIVDR
                     HWLSSLAHAFLLDGGAGCVTGLILPLELETRAQLWFEQFGGFGKGFQRHSFDLGPNRP
                     PDALFPYAAGRFGSGANTAFRTGILRQLGGFAVELGAGTPAHGGEDLDAYLNVIRAGH
                     RLLYEPRALLWHPHLRSLRALSRQIHRYGVGLSAALTKRFVASREERRQMVRRLPAGL
                     AYAVDPRSAKNAGKDPSYPGWLTLLELAGMLRGPVALALARWSA"
BASE COUNT          296 a          716 c          765 g          232 t
ORIGIN      
        1 gggcgactca cgggtgccgg tgtgccggtc gtaccgtcgt ggcgcgcagc tgacggccca
       61 cgagacccgc tggtcgaagt agcgctgctc cgcgttctcg aggcgtgcgg acggcaccag
      121 gccggtgacg caggcgaccg agggcgacct gccgaacccc cggaccacgc cgtcgagcca
      181 gcggggatcc acgatcacgt cgtcgtcggt gaaggcgacg agctcgccgc gcgcctcggc
      241 cagcccccgg ttccgggcgc acgacagacc gggtcggggc tcccgcacgt aacggacccg
      301 ggacccggtg cctgaccgct cccggacgac ctcgagggtc tcccgcgtcg tcggcgcgtt
      361 gtcgacgacg atgacctcga acgcgtcgta gtgcaccgcg gccacggcgt cgaggcagcg
      421 gaccaggctc tgcgggcggt cccgggtgca gacgacgacc gtcaccgtcg ggtggccggg
      481 accgccggcc ggtgcctgga ggcagcgtgg acgccgctcg gcgcccagac cggccaccgg
      541 gatggacgtg acgcggccga gcccgtcctc ggtcagatgg gcgttcacct cggcgccgag
      601 ctcctcccag aggagccgag ccacccgcac ggggtccggc cacccatcca ccagcggcac
      661 ggtcatgaag ccgagcggct ggccgtgcag ccggacgagc agccgggtgg ccgagtagga
      721 caggccgtcc ctggctggga cggtgaggtc gcagatcggc tcggccagct cgagctggcc
      781 gacgagcacg gcgccgccgg ccgctaccgg cttgccggcg ggcccgccgt cgagcgccac
      841 gtggcgggtc atctcggatc gtcggccggg gtcgagacca ggtcgagcac caggacggtg
      901 gccgacacgg cggcgagccc ggccatgagg cgcagcgggt gccaggctcc cagccatgcc
      961 atgagcaggg aggcggcggc gcagatcgcg aggctgagca cgacggtcat cgccagtacc
     1021 tcgtcgggtt cccggaacgg caggcgggtg acgagcgccc cgcccggcac cagacaggcg
     1081 aacgccaggg ccgtgagcac gcgcggcagg ccgtcgacgt cgagcacgac gagcagggcg
     1141 agcagggccg tcacgcccag caggctcacg tcgaagacgg ccatccaggt ctgccggtgg
     1201 gattcctggt ggttcatgct catgccgacc accgggcgag cgcgagcgcg accggcccgc
     1261 gaagcatgcc ggcgagctcc aggagggtga gccacccggg gtagctcggg tccttgcccg
     1321 cgttcttcgc ggaccgggga tccacggcgt aggccagtcc cgcgggcagg cgccggacca
     1381 tctgcctgcg ctcctcccgg ctcgcgacga agcgcttggt gagcgcggcg ctgagcccga
     1441 ccccgtaccg gtggatctgc cgcgagagtg cccgcagcga gcggaggtgc gggtgccaca
     1501 ggagtgctcg cggctcgtag agaagccggt gaccggcgcg gatgacgttg agataggcgt
     1561 cgaggtcctc gccgccgtgc gcgggggtgc ccgcgcccag ctcgacggca aagcctccca
     1621 gctgccgcag gatgccggtc cggaacgccg tgttggcccc ggacccgaag cgtccggcgg
     1681 cgtacgggaa gagcgcgtcc ggcgggcggt tcggcccgag gtcgaacgag tgccgctgga
     1741 agcccttgcc gaagccgccg aactgctcga accagagctg cgctctggtc tccagctcga
     1801 gggggaggat cagccctgtc acgcacccgg cgccgccgtc cagcaggaac gcgtgcgcga
     1861 gcgacgacag ccagtgccgg tcgacgatca cgtcgtcgtc ggtgaacgcg acgatctccc
     1921 cggtgaccgc ggacagcgcc tcgttgtgcg ccagggccag ccctggccgg gccgcccgca
     1981 ggtagcgcag gccgaacggc cagtcctgg
//
LOCUS       DASPYY010000195         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2704859, whole genome shotgun sequence.
ACCESSION   DASPYY010000195 DASPYY010000000
VERSION     DASPYY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704859"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VEP73_02945"
     CDS             complement(<1..166)
                     /locus_tag="VEP73_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYT25427.1"
                     /translation="MERVFITGIGTVNPLGLDAPSTWEAMLEGRSGIDTIAAFDPSPY
                     PARIAGEVRGF"
     gene            complement(279..515)
                     /locus_tag="VEP73_02950"
     CDS             complement(279..515)
                     /locus_tag="VEP73_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25428.1"
                     /translation="MRDPNAIEPQDDFTDLADEPDESELELADQLPDDLDPHEMANTI
                     EDELVAEEGEELGLDPDLAVFAAREELADEDDEF"
     gene            complement(602..2032)
                     /locus_tag="VEP73_02955"
     CDS             complement(602..2032)
                     /locus_tag="VEP73_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018732312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYT25429.1"
                     /translation="MARGQVLALDLGTSSVRALVFDADGKAVPDLLARRPTHLAIDDG
                     GKAELDPEQVVGAIGECLDELRDGGHLDPVEDVAISSAWHSVIAADRAGRPLTGALTW
                     ADTRAAGLVPALRERADPDRLLAETGALPHTTFWTAKVPWLARTVSPPPARYLGLAEY
                     VTGRLLGDEGASISMASGTGLLGLETMAWSPEALDLADVGPGALPEVLAPGWSGRLRP
                     EGARRWPALAGATWHPVTGDGAASNVGAGCVTPEYANVNIGTSAAIRAVHDDPGAPLH
                     RELWRYRVDERRLVTGAAFSGGGNLWAWAKQVLRLPGDDEMEAALASVPPGAEGVSVM
                     PYHAGARVPLDLLAGSGAILGLSLATTAVEIVAATLEAVCFGLAAGLEALEAALPAPP
                     ELVASGGAVVASPWWQQTMANVFDRPVLVTDEPEASARGAALVAVGRVTSPETKHVVE
                     PDPKAVEAERAARSVHEDFAARLGYA"
     gene            complement(2043..2603)
                     /locus_tag="VEP73_02960"
     CDS             complement(2043..2603)
                     /locus_tag="VEP73_02960"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HYT25430.1"
                     /translation="MAETRAAPGRLRLVQQFVNTYDLEDDVDQVDDPERLRAWLAGHG
                     LLGAGERLGAADVQRAVEVREALRAVLLANAGAPLEAGAVETLNRASRNSRLIVHFGP
                     DGRATLEPEPLGVEAAIGRILAAVHTSMADGTWARFKACRNEECRWAYYDHSKNRSGR
                     WCTMEVCGNAMKARSYRRRKRAAAHG"
     gene            complement(2738..3754)
                     /gene="speB"
                     /locus_tag="VEP73_02965"
     CDS             complement(2738..3754)
                     /gene="speB"
                     /locus_tag="VEP73_02965"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYT25431.1"
                     /translation="MRSQLDSGYQGLATFSGRPLLTEPEQLEDWAPDVAVVGAPWDGS
                     TTNRPGARFGPRALRALAYHPGGFHLDLGLEVFELLQVVDYGDAICSHGLWEPSKEAI
                     GRRVGEVASRGVTPVILGGDHSITWPAATAVAEHHGFGNVGVVHFDAHADTADSIDGN
                     LASHGTPMRRLIESGAVPGRNFVQVGLRGYWPPADVFAWMREQGMRWHLMHELWERGA
                     PAVIEDAIAEALDGADALYLSVDIDVLDPGFAPGTGTPEPGGMAPADLLRAVREIAMR
                     TNLVAMDVVEVAPAYDRADLTVNNAHRVVLEALAGLAWKELPPAERASPPRPPHPPVA
                     DATT"
     gene            3975..>4397
                     /locus_tag="VEP73_02970"
     CDS             3975..>4397
                     /locus_tag="VEP73_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYT25432.1"
                     /translation="MRRLVLWDVDGTLVVAGQVGRDAFSDAFLSVVGRAPEDVTARMI
                     EMAGRTDPEIALEFLAAHRVADGETWLPAFTEALVAALGTKAAVIREHGRALPGAAAA
                     LSALRRIPGVVQSLLTGNVQPNALVKLAAFGLERDVDFD"
BASE COUNT          545 a         1616 c         1645 g          591 t
ORIGIN      
        1 cgaagccgcg cacctccccg gcgatgcgcg ccgggtacgg ggacgggtcg aaggcggcga
       61 tcgtgtcgat gccgctgcgt ccctcgagca tcgcctccca cgtcgacggg gcgtcgagcc
      121 ccagcgggtt gacggtgccg atgccggtga tgaagacgcg ttccatctcg ggccaagggt
      181 agcaggtggc ggcctcgccg gaaccgcccg aactgcccgg ggcgcctgcg gcgcggctcg
      241 ttcagactcc ggccggcaac attcgccggc cgggccggtc agaactcgtc gtcctcgtcc
      301 gccagctcct cacgggcggc gaacacggcg aggtccgggt cgaggccgag ctcctctcct
      361 tcctcggcga cgagctcgtc ctcaatcgtg ttggccatct cgtgcgggtc gaggtcgtcg
      421 ggaagctggt cggccagctc gagctcggac tcgtccggtt cgtcggcgag gtcggtgaag
      481 tcgtcctgcg gctcgatcgc gttggggtct cgcacaggct ggctcccttc acgtcacggt
      541 cggggcgctg cagacacgtc agggcagatc gaagtcgatc gaagtcagtc agaggtcgat
      601 gttaggcgta gccgagcctg gcggcgaaat cctcgtggac ggagcgggcc gcgcgctccg
      661 cctccaccgc cttcgggtcc ggctcgacga cgtgcttggt ctcggggctc gtgaccctgc
      721 cgacggccac cagggcggcg ccgcgcgccg acgcctccgg ctcgtcggtg accaggacgg
      781 gacggtcgaa cacgttggcc atcgtctgct gccaccaggg cgaggcgacc acggcgccgc
      841 ccgaggccac cagctcgggc ggcgcgggca gggcggcttc gagcgcctcg aggcccgccg
      901 cgaggccgaa gcagaccgcc tcgagcgtcg cggcgacgat ctccaccgcg gtggtggcga
      961 gcgacaggcc gaggatcgcc cccgacccgg ccagcaggtc gagcggtacc cgcgccccgg
     1021 cgtggtaggg catgacgctg acgccctcgg ccccgggcgg gaccgaggcg agggcggcct
     1081 ccatctcgtc gtcgccaggc agccgcagca cctgcttcgc ccacgcccac aggttgccgc
     1141 cgccggagaa cgcggccccg gtgacgagcc gccgctcgtc gacccggtag cgccacagct
     1201 cgcggtgcag cggcgcgccg gggtcgtcgt ggaccgcccg gatggccgcc gacgtgccga
     1261 tgttgacgtt cgcgtactcg ggggtcacgc acccggcgcc gacgttggag gcggcgccgt
     1321 cgccggtgac cgggtgccag gtcgccccgg cgagcgcggg ccagcgcctt gctccctccg
     1381 gccgcagccg gccggaccag ccgggggcga gcacctcggg aagggcgccg gggccgacgt
     1441 cggcgaggtc gagggcctcg gggctccacg ccatcgtctc gaggccgagc agcccggtgc
     1501 cggacgccat ggagatcgag gcgccctcgt cgccgaggag ccgcccggtc acgtactcgg
     1561 cgagcccgag gtacctggcc ggaggcgggg agacggtccg ggccagccag ggcaccttcg
     1621 cggtccagaa cgtggtatgg ggcagcgcgc cggtctcggc gagcaggcgg tcggggtcgg
     1681 cgcgctcgcg caaggcgggg acgagaccgg cggcgcgggt gtcggcccac gtcagcgcgc
     1741 cggtgagggg ccgcccggca cggtcggcgg cgatcacgga gtgccaggcg ctcgagatgg
     1801 cgacgtcctc gacggggtcg aggtggccgc cgtccctgag ctcgtccagg cactcgccga
     1861 tggcgcccac cacctgctcg gggtccagct cggccttgcc gccgtcgtcg atggcgaggt
     1921 gggtcggccg cctcgcgagc aggtcgggga cggccttgcc gtcggcgtcg aagacgagcg
     1981 ccctgaccga ggacgtcccc aggtccaggg cgagcacctg tcctcgcgcc acgtccacct
     2041 cctcagccgt gcgcggcggc acgcttgcgg cgccggtagc tgcgggcctt catcgcgttg
     2101 ccgcagacct ccatcgtgca ccagcgaccc gagcggttct tggagtggtc gtagtaggcc
     2161 cagcggcact cctcgttgcg gcacgccttg aagcgggccc aggtgccgtc ggccatcgag
     2221 gtgtggacgg cggcgaggat gcggccgatc gcggcctcga cgcccagcgg ctccggctcg
     2281 agcgtcgcgc gcccgtcggg gccgaagtgg acgatcaggc gggagttcct cgacgcgcgg
     2341 ttcagcgtct ccaccgcccc ggcctcgagc ggggcaccgg cgttggcgag caggacggcc
     2401 cggagcgcct cgcgcacctc gacggcccgc tggacgtcgg ccgccccgag gcgctcgccc
     2461 gcgccgagca gcccgtgacc ggccagccag gcccggagcc gctcggggtc gtcgacctgg
     2521 tcgacgtcgt cctcgaggtc gtaggtgttc acgaactgct gcaccagccg gagcctgccc
     2581 ggtgccgccc ttgtctccgc catggtccca ggtcctcgcc cgcagcctgc cgtccatcca
     2641 tctccatcac cagtttaccc acatgagcgg tcatgaagag cgaaggcgcc tcggatccgc
     2701 gtcacccagt cactatcgaa gaagcttaga tagtgactca cgtggtagca tcggcgacgg
     2761 gaggatgcgg cgggcgcggc ggcgaggccc gctcggcggg cgggagctcc ttccaggcca
     2821 ggccggcgag ggcctcgagc acgacccggt gggcgttgtt gaccgtcagg tcggcccggt
     2881 cgtacgcggg tgcgacttcg acgacgtcca tggccaccag gttcgtccgc atggcgatct
     2941 cccgcaccgc gcgcagcagg tcggctgggg ccatgccgcc gggctcgggg gtgccggtgc
     3001 ccggggcgaa gccggggtcg agcacgtcga tgtcgaccga caggtagagc gcgtcggcgc
     3061 cgtcgagcgc ctcggcgatg gcgtcctcga tcaccgcggg cgcgccccgc tcccacagct
     3121 cgtgcatgag gtgccagcgc atgccctgct cgcgcatcca cgcgaagacg tcggccggcg
     3181 gccagtaccc gcgcagcccc acctgcacga agttccggcc gggcaccgcg cccgactcga
     3241 tcagccggcg catcggcgtg ccgtggctcg cgaggttgcc gtcgatggag tcggcggtgt
     3301 cggcgtgcgc gtcgaagtgc accacgccga cgttcccgaa gccgtggtgc tcggccacgg
     3361 cggtcgcggc cggccaggtg atcgagtggt cgcccccgag gatcacgggc gtgacgcccc
     3421 ggctcgcgac ctcgccgacc cgccgcccga tcgcctcctt cgacggctcc cacaggccat
     3481 ggctgcagat ggcgtcgccg tagtccacca cctgaagcag ctcgaagacc tcgagcccga
     3541 ggtccagatg gaacccgccc gggtggtacg ccagggcgcg gagcgcccgc ggtccgaagc
     3601 gcgccccggg ccggttggtg gtcgagccgt cccagggggc gccgaccacc gccacgtccg
     3661 gcgcccagtc ctcgagctgc tccggctcgg tcagcagcgg gcgcccggag aacgtggcga
     3721 gtccctggta gccggagtcg agctggctgc gcatcccggg cggcagccgg cggtccggcc
     3781 cggtcacgga cgtcacgtgc gctcctcgct cgtcggggtc cagtccgccg gttctccgag
     3841 tccggttcgc cggacgtccg gcggcgtcat gctaccgccg ggcgtgcccc tcgccccccc
     3901 ggccggcgcc ggcgtcggcc cagccgttac gatcggggcc ggcgtgacga tcggcgggca
     3961 gctcgggagg tcatgtgcgc aggctggtgc tctgggacgt cgacggcacg ctggtcgtgg
     4021 ccgggcaggt ggggcgcgac gccttctcgg acgccttcct ctcggtcgtc ggtcgggccc
     4081 ccgaggacgt cacggcccgc atgatcgaga tggcgggacg gaccgacccg gagatcgcgc
     4141 tcgagttcct cgccgctcac cgggtcgccg acggggagac ctggctgccg gccttcaccg
     4201 aggcgctggt cgccgcgctc ggcaccaagg cggcggtgat ccgcgagcac ggccgcgcgc
     4261 tgcccggcgc cgcggccgcc ctctccgccc tgcggcggat ccctggcgtc gtccagtcgc
     4321 tgctcaccgg caacgtccag ccgaacgccc tggtgaagct cgcggcgttc gggctcgagc
     4381 gcgacgtcga cttcgac
//
LOCUS       DASPYY010000196         4958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_176627, whole genome shotgun sequence.
ACCESSION   DASPYY010000196 DASPYY010000000
VERSION     DASPYY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176627"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VEP73_02975"
     CDS             <1..873
                     /locus_tag="VEP73_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT25433.1"
                     /translation="AGIDALLDLAMAANADPGLRPDPAGAPVPLADARLLAPVTDPPA
                     VRHFDAFEQHVRTARARRGREMHPDWYELPAFYFSNPHAIVGPGEPVAAPPRSAELDY
                     ELEVACVLGAGADEVGLDDADRLVAGFMVMNDWSARDVQRQELQLSMGPAKGKDFATS
                     LGPWLVTTNEFAPRGLRTVPRATMTAWVNGVEYSRASLDGLWWSFAEMLAYAAEAAPV
                     RRGDVLASGTCGTGCIVELSLVHGSEKFPYLQPGDEVELEVDGLGVLANRVQAAGGAP
                     FSPDPERIRPRLGP"
     gene            870..1370
                     /locus_tag="VEP73_02980"
     CDS             870..1370
                     /locus_tag="VEP73_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYT25434.1"
                     /translation="MTVPPAPAPGDRAARLLAAAGRIRAHVADWPPGVLTTPDADTGE
                     RWDAGQLLAHVAEMLPYWTQEAELVAASGGGVAFGRVKTDPDRVAAIERDRREHLSLL
                     LGRIDEGVAEVLQLVDGLDGEALAREGTHQTLGRMTVAGIVDRFLVDHLEEHAEQIEG
                     VVPPVP"
     gene            1461..2258
                     /locus_tag="VEP73_02985"
     CDS             1461..2258
                     /locus_tag="VEP73_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT25435.1"
                     /translation="MTVQPDSSQYVLDVAEADFEDQVLRRSSQVPVVVDFWAEWCAPC
                     RSLGPILERAVARHEGDVVLARVDVDQAEQLAMAFGIQGIPAVKAFVDGQVVDEFVGA
                     QPAAVVERFVEGLVPSLSDRVAAEAATLGPEQAARLYREALEADPDSVAARVGLAGLA
                     LDRGDAAEALELLAPVEYAPEAAEVLAAVRLAREGADQGSPFAAAARLATDGAPETAL
                     EELLAAVREGGDRRDRARQLMLDVFRLLGDTHPLTMRYRRSLSAALF"
     gene            2359..3777
                     /gene="proS"
                     /locus_tag="VEP73_02990"
     CDS             2359..3777
                     /gene="proS"
                     /locus_tag="VEP73_02990"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HYT25436.1"
                     /translation="MAKQAVLTPQGEDFPQWYQDVVARAELAENGPVRGTMVIRPWGY
                     GIWELMQADMDRRIKATGVQNAYFPLFIPMSFLEKEKEHVEGFKPELAVVTYGGGEEL
                     AEPIVVRPTSETVVNHLFAKWIQSHRDLPLLINQWANVVRWELRPRILLRTTEFLWQE
                     GHTAHATYEEAQAEAMQMLEVYRSFMEDTLAVAVAVGEKSPRERFAGADRTYTLEGLM
                     RDGKALQMGTSHNLGHNFARSFDIQYLSADGTREYVATTSWGSSTRMLGAVIMAHGDD
                     HGLRLPPAIAPHQVVLVPLAEGEVARAVEKLAAELAADGVRTHVDTRFHLSFGRRSVD
                     WELKGVPVRVELGPRELQAGQATLVRRDTRAKSPVPLDGAARAVRDLLDELQRSLHAE
                     SLAFRTAHTWPVTDLAELRRRVGDGGFFLVSWCGKDACEEALGDGTGASIRAIVGEDP
                     PTPVCLVDGAPATHAVLVARAY"
     gene            3900..4313
                     /locus_tag="VEP73_02995"
     CDS             3900..4313
                     /locus_tag="VEP73_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYT25437.1"
                     /translation="MSIQTRSAPSNPALLRHAEERAGSLQNRIADAITRFAGSMWFVY
                     IHVLWFAAWIVFRVEHYPFGLLTMIVSLEAIFLSTFVMISQNRADEKRQALADHQWKM
                     VQEEDQQNEELLGLSKQILELTRAIHTMTSARTRD"
     gene            4366..4626
                     /locus_tag="VEP73_03000"
     CDS             4366..4626
                     /locus_tag="VEP73_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25438.1"
                     /translation="MRTIAHFWPITRAHIYAELGQEVRRHSLLLKVFFGERIPPERLA
                     GLLAEERARLEANRDCYAATVDEVEPLVAASVAADVPADRSR"
     gene            4627..>4958
                     /locus_tag="VEP73_03005"
     CDS             4627..>4958
                     /locus_tag="VEP73_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25439.1"
                     /translation="MFKENRRTKLLTLGLSCLGLFMVLLDLTIVNNALPSIQRHLGAS
                     VSGLQWIIDAYTLVFASLLLTTGALSDRQGRKRWFLIGLSVFTAGSALCGLAPNLGVL
                     IGARALQAV"
BASE COUNT          668 a         1845 c         1760 g          685 t
ORIGIN      
        1 gccgggatcg acgccctgct cgacctggcc atggcggcca acgccgaccc cgggctgcgc
       61 ccggacccgg cgggggctcc ggtgccgctc gccgacgcgc ggctgctcgc gccggtgacc
      121 gacccacccg ccgtgcggca cttcgacgcc ttcgagcagc acgtgcgcac ggcgcgggcc
      181 aggcgggggc gggagatgca ccccgactgg tacgagctgc ccgcgttcta cttctcgaac
      241 ccgcacgcca tcgtcggacc cggcgagccc gtggccgcgc cgccgaggtc ggccgagctc
      301 gactacgagc tcgaggtcgc atgcgtgctc ggggccggcg ccgacgaggt cggcctcgac
      361 gacgccgacc ggctggtcgc cgggttcatg gtgatgaacg actggtcggc ccgggacgtc
      421 cagcgccagg agctgcagct gtcgatgggg ccggccaagg gcaaggactt cgccacctcg
      481 ctcggcccat ggctcgtgac caccaacgag ttcgcgcccc gcggcctccg cacggtcccc
      541 cgcgccacca tgaccgcctg ggtcaacggc gtcgagtact cccgtgccag cctcgacggg
      601 ctgtggtgga gcttcgccga gatgctcgcg tacgcggccg aggcggcgcc ggtgcgccgc
      661 ggcgacgtgc tcgcctcggg tacctgcggg accggctgca tcgtcgagct gtcgctggtc
      721 cacggcagcg agaagtttcc gtacctgcag cccggcgacg aggtcgagct ggaggtcgac
      781 gggctcgggg tgctggccaa ccgcgtgcag gccgccggcg gcgccccgtt cagccccgac
      841 ccggagcgca tccggccgag gctcggcccg tgaccgtgcc gccagctccg gctccgggcg
      901 accgggccgc gcgcctgctc gcggccgccg gccgcatccg cgcccacgtc gccgactggc
      961 cgcccggcgt cctgaccacc ccggacgcgg acacggggga gcgctgggac gcgggccagc
     1021 tgctcgccca cgtggccgag atgctgccgt actggaccca ggaggccgag ctggtcgcgg
     1081 cctcgggcgg cggggtggcc ttcggccgcg tcaagaccga ccccgaccgc gtcgccgcga
     1141 tcgagcggga ccgccgcgag cacctctccc tgctgctcgg tcgcatcgac gagggggtgg
     1201 cggaggtgct ccagctcgtc gacgggcttg acggagaggc tctggcccgc gagggcaccc
     1261 accagactct cgggcggatg accgtcgccg ggatcgtcga ccggttcctc gtggaccacc
     1321 tcgaggagca cgccgagcag atcgagggcg tcgtcccgcc ggtcccctga gggacccggc
     1381 gcgcatggag cggagcgagc gcccaggggc cgccgggccg ccgggttgca cggcgcgccg
     1441 caggagcgta cctacagggt atgaccgtgc aaccagactc cagccagtac gtcctcgacg
     1501 tcgccgaggc cgacttcgag gaccaggtcc tgcgccgctc gtcccaggtc ccggtggtgg
     1561 tcgacttctg ggcggaatgg tgcgcgccct gccgcagcct cggcccgatc ctcgagcggg
     1621 ccgtcgcccg gcacgagggc gacgtcgtgc tcgcccgggt cgacgtcgac caggcagagc
     1681 agctcgcgat ggcgttcggc atccagggca tcccagccgt gaaggcgttc gtcgacggcc
     1741 aggtggtcga cgagttcgtc ggcgcgcagc ccgccgccgt ggtcgagcgc ttcgtcgagg
     1801 ggctcgtgcc gagcctgagc gaccgggtcg ccgccgaggc ggccactctc ggtcccgagc
     1861 aggccgcccg gctgtaccgg gaggcgctcg aggccgaccc ggacagcgtg gccgcccggg
     1921 tcgggctcgc cggcctggcc ctggatcggg gcgacgccgc cgaggcgctc gagctgttgg
     1981 ccccggtcga gtacgcgccc gaggcggccg aggtgctggc cgcggtgcgg ctcgcccgcg
     2041 agggcgccga ccagggctcg ccgttcgcgg ccgcggcccg gctggcgacc gacggtgcgc
     2101 ccgagaccgc gctggaggag ctgctcgccg cggtccgcga gggcggcgac cgtcgcgacc
     2161 gtgcccggca gctcatgctc gacgtgttcc gcctgctcgg tgacacccac cccctcacca
     2221 tgcggtaccg tcggagcctc tccgcagcgc tgttctaggg acgccggccg ccccggctcc
     2281 cgaacggctt tgcccggctc gccacgtctg ggtacgctgc tctttcgagt tccgccggat
     2341 cgaggaggcg cagcgccagt ggccaagcag gccgtgctca ccccccaggg ggaggacttc
     2401 ccccagtggt accaggacgt cgtcgctcgg gccgagctgg ccgagaacgg cccggtgcgg
     2461 gggaccatgg tcatccgacc gtgggggtac gggatctggg agctcatgca ggccgacatg
     2521 gaccgccgca tcaaggccac cggcgtgcag aacgcctact tcccgctctt catccccatg
     2581 agctttctcg agaaggagaa ggagcacgtc gaggggttca agcccgagct cgcggtggtc
     2641 acctacggcg gcggggagga gctcgccgag ccgatcgtgg tccggccgac ctccgagacg
     2701 gtggtcaacc acctgttcgc gaagtggatc cagtcgcatc gcgacctgcc gctgctcatc
     2761 aaccagtggg ccaacgtggt ccgctgggag ctgcggcccc gcatcctgct gcgcacgacc
     2821 gagttcctct ggcaggaggg gcacaccgcc cacgccacct acgaggaggc gcaggcggag
     2881 gccatgcaga tgctcgaggt gtaccggtcg ttcatggagg acaccctggc cgtggccgtc
     2941 gcggtggggg agaagtcccc ccgtgagcgc ttcgccggcg ccgaccggac ctacacgctc
     3001 gagggcctga tgcgggacgg caaggcgctg cagatgggga cgagccacaa cctcgggcac
     3061 aacttcgccc gctcgttcga catccagtac ctgtccgccg acggcacgcg cgagtacgtc
     3121 gccaccacct cgtggggctc gtcgacccgc atgctcggcg cggtcatcat ggcccacggc
     3181 gacgaccacg ggctgcgcct gccgccggcc atcgccccgc accaggtcgt gctcgtgccc
     3241 ctcgccgagg gggaggtcgc ccgggcggtg gagaaactgg cggcggagct cgccgccgac
     3301 ggggtccgca cccacgtcga cacccggttc cacctgtcgt tcggccgccg ctcggtcgac
     3361 tgggagctca agggcgtccc cgtccgggtc gagctcggcc cccgcgagct ccaggccggc
     3421 caggcgacgc tcgtccggcg ggacaccagg gccaagtcgc cggtcccgct cgacggcgcc
     3481 gcccgcgccg tccgggacct gctcgacgag ctgcagcggt ccctgcacgc cgagtcgctg
     3541 gcgttccgca ccgcgcacac ctggccggtg accgacctgg ccgagctcag gcgccgggtc
     3601 ggggacggag ggttcttcct ggtctcctgg tgcggcaagg acgcctgcga ggaggcgctc
     3661 ggcgacggca cgggcgcgtc gatccgggcc atcgtcggcg aggacccgcc cacgcccgtg
     3721 tgcctggtgg acggcgcccc cgcgacccac gccgtcctgg tcgccagagc ctactgagcc
     3781 ccccgccgcc ggccggtccc ggcctcaggg gttcgcgacc tcgcccgctt ccgtcaccgc
     3841 ctgccttacc ctgaaccgtc ggcccacctc ccgcgggccg ggaaccggaa ggagagagcg
     3901 tgagcattca gacgcgttcg gcaccgagca acccggcgct gctccggcat gccgaggagc
     3961 gagctgggag ccttcagaac cgcatcgccg acgcgatcac gcggttcgcc ggctcgatgt
     4021 ggttcgtgta catccacgtg ctgtggttcg ccgcgtggat cgtcttcagg gtcgagcact
     4081 acccgttcgg cctgctcacg atgatcgtgt cgctggaggc gatcttcctg tcgacgttcg
     4141 tgatgatcag ccagaaccgg gccgacgaga aacgccaggc cctggccgac caccagtgga
     4201 agatggtgca ggaggaggac cagcagaacg aggagctgct cggcctctcc aagcagatcc
     4261 tcgagctcac cagggccatc cacacgatga cctcggcgcg gacgcgggac tgacgtcgtt
     4321 ctctcgtcac gtgaccgtcc agccttgcat agtcaaggct tgactatgcg gacgatcgcc
     4381 cacttctggc cgatcacccg cgcccacatc tacgccgagc tgggccagga ggtccgccgc
     4441 cactcgctgc tcctcaaggt cttcttcggc gaacgcatcc cccccgagcg tctcgccggc
     4501 ctgctcgccg aggagcgcgc ccggctcgag gccaaccgcg actgctacgc ggccaccgtc
     4561 gacgaggtcg agccgctggt cgcagcaagc gtggcagccg acgtgccagc cgatcggagc
     4621 cgctgaatgt tcaaggagaa ccgcaggacc aagctgctca ccctggggct gagctgcctc
     4681 gggctcttca tggtgctgct cgacctgacc atcgtgaaca acgccctgcc gtcgatccag
     4741 cggcacctcg gcgccagcgt ctccggcctg cagtggatca tcgacgccta cacgctggtg
     4801 ttcgccagcc tgctgctcac caccggcgcg ctcagcgacc ggcagggccg caagcgctgg
     4861 ttcctgatcg gcctctcggt cttcacggcc ggctcggccc tttgcggcct tgcccccaac
     4921 ctcggggtgc tgatcggcgc gcgggcgctg caggcggt
//
LOCUS       DASPYY010000197         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_35415, whole genome shotgun sequence.
ACCESSION   DASPYY010000197 DASPYY010000000
VERSION     DASPYY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35415"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..704
                     /locus_tag="VEP73_03010"
                     /pseudo
     CDS             357..704
                     /locus_tag="VEP73_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            824..1138
                     /locus_tag="VEP73_03015"
     CDS             824..1138
                     /locus_tag="VEP73_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25440.1"
                     /translation="MRGRFGEVWGVLSIADPPERSTGHVEIAAGSIDTGQEGRDARHR
                     RHARGLPRRGYRLPRRRPVPFPHVPHRCRCRARATGAARREGELTLRAVRCAGGELRQ
                     VA"
     gene            1113..>4114
                     /locus_tag="VEP73_03020"
     CDS             1113..>4114
                     /locus_tag="VEP73_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25441.1"
                     /translation="MESCGRSPDPADEDGQTAAGLFQSEEAKRILFAMAGTVATSRFV
                     GRTAELGRLEAAFAYVGGGEPVTVCVGGEAGVGKTRLVTRFAGQVRGSGGQVLLGGCI
                     ELGEGSLPYAPVVEALRGLGRGLEPAALDELVGPGRPLLARLLPELGQGEESTSAGLA
                     VGSSAQARLFEAFLALLERLAGRSPTVLVVEDLHWADRSTLDLLTFLVRNLRAALLLV
                     LTYRTDELHRRHPLRPFLAGLDRSGRAERLEVGRFDRVDVADLLAGILGTRPDDELVD
                     RIYRRSEGNAFFAEELLAATHQGNGRSLPPSLENVLLSRVQVLPEDAQATLRIVAAAS
                     GRVEHELLAAVSELPEADLLAALRAAVAHQMLVPDPATETYAFRHALTQEALYAELLP
                     GERARLHAAFARVLTERPDLAEPDRLAVPARLAYHWVRAHDPARALPAAVEAGLQSEA
                     AYGFADAQRHFEMALELWDQVADAEERLGLDRAAVLQHAAESAYLAGDPNRAITLTRA
                     ALVDVDAAKDPVRAGLLHGRLGGYLRATGGEGAFAEYEAAVRLVPASPPLAERAQVLA
                     AFGEALIGQGHYRESRDLCEEAIAIARQIGALAEEGDARRALGVDLAFLGDLEAGVGQ
                     LSEARRIAEAVGKVDEVARAFATLSGLLEAFGELEAATALALEGAELAASQGLGRWHS
                     PFLTATAARALFALGRWDDADLLLHRAADRVAPELAATRVYIHTARSQLDFAQGLAAS
                     AAEHLAVAREAYAQTVTQPWFATPLFTATAELAMLEGRLNDADAAVAEGLRVAGANLI
                     FGAPLYALGLRAAADRAELASARRTGDEVLEARRAGSALASELRARMSPEGADGTVPT
                     PRTEAQAVLCEAELARLGGRSDPDLWAAAANVWERLGEPYPTAYARWREAEALLLGGL
                     VREQVEPSLRAAHATASELGAAPLRAEIEALARRGRLNLGTDPATTPPPAPEPPSPLN
                     RLGLTAREQEVLALVAIGRTNRQIAETL"
BASE COUNT          516 a         1542 c         1502 g          554 t
ORIGIN      
        1 agggcagcta gcggctcgtg gtgtcttggt tcggtcgagg gactacctca ctgccgactg
       61 cccttcgtca ggccgcccaa cttcgcccgt gacggtcccg cgcgaccagc gaagatgcag
      121 gttggcatgg cgctcacccc caactcgctc gctgcgatcg ggcggacatg gcctctgaga
      181 ccacccgagc cggtcgccgg gtggctcctg gctacgacgc cgggcgcgag aagttggggt
      241 gcgacgaccc gcggatctag aaggatcgtc cgatccctcg gcgggacccg tgacctacgg
      301 tggccccacg cgacccggca cgagcggtcg cactcccccg aaaggagcga ccatcgatgg
      361 atgcgggcac ggtgatcgtc gaggctcacg ccgacctgga gcggctcttc gccgcctacg
      421 acggcgccga tggtggcagg gctgcgcgct tcagcacctt cggcgccatc gcgagcgccc
      481 tgggggtgct tgaggccatc gagggcgaac tgctctaccc cgcgctgcag gcgcagacga
      541 gccggtacga cggcgacatc gagcggcagc tcgagcaggc tcatctcctt gacctgctgg
      601 cggtggagct ggccgcgatg gtcccgaccg acccgcgcta cgacgcgaag gtgcaggtcc
      661 tcctgcaggt cttccgcctc cacatgcggg acgaggaggc gctcatcctg ccggaggtcc
      721 gacgccgcct cagcagccgg ccccggcacc cggggtcgac atctgggaca tcgaccccgt
      781 ctactccacc gtacagttcg ccgtccggca cacgttcacc aggatgcgcg gacggttcgg
      841 cgaagtctgg ggcgtcctgt cgatcgccga cccgcccgag cgctcgaccg gccacgtcga
      901 gatcgcggcc ggcagcatcg acacgggcca ggaggggcgc gacgcacgcc atcgccgcca
      961 cgcccgagga ctcccgcgtc gcggctaccg actccctcga cgccgaccgg ttccctttcc
     1021 tcacgttccg catcgctgtc ggtgtcgcgc gagagcgacc ggcgctgcgc gacgagaggg
     1081 cgagctgaca ctccgcgcgg tccggtgcgc cggtggagag ctgcggcagg tcgcctgacc
     1141 ccgcggacga ggacggccag acggccgccg gtctgttcca gtcagaggag gcgaagcgca
     1201 tacttttcgc catggccggg accgtcgcga cctcgcgctt cgtcggtcgc acagctgagc
     1261 tgggccgtct cgaggcggcg ttcgcgtacg tcggcggcgg cgagccggtg acggtgtgcg
     1321 tgggcggtga ggccggcgtc gggaagactc gcctggtcac ccggttcgcc ggtcaggtcc
     1381 gcgggagcgg cgggcaggtg ctgctcggcg gctgcatcga gctcggcgag gggtcgctgc
     1441 cctacgcacc cgtggtcgag gcgctccgcg gtctgggccg ggggctggaa ccggcggcgc
     1501 tggacgagct ggtcggaccc ggacggccgc tgctggcccg cctgctgccc gagctcgggc
     1561 agggcgagga gtcgacgtcg gccgggctcg ccgtcggctc gtcggcgcag gcccggctgt
     1621 tcgaggcgtt cctcgccctg ctggagcgcc tcgccggccg gtccccgacc gtgctggtgg
     1681 tggaggacct gcactgggcc gatcgctcga ccctggacct gctcaccttc ctcgtccgca
     1741 acctgcgggc cgcgctgctc ctcgtgctga cctaccgcac cgacgagctg caccggcggc
     1801 atcccctgcg gccgttcctg gccggactgg accgcagcgg acgggccgag cggctcgagg
     1861 tgggccgctt cgaccgtgtc gacgtcgccg acctgctggc cggcatcctc gggacgaggc
     1921 cggacgacga gctcgtcgac cggatctatc gccgctcgga gggcaacgcg ttcttcgcgg
     1981 aggagctgct cgccgccacc caccagggca acgggcgcag cctgcccccg agcctcgaga
     2041 acgtcctgct gagccgcgtc caggtcctcc ccgaggacgc gcaggcgaca ctccggatcg
     2101 tggccgcggc cagcggccgg gtcgagcacg agctgctcgc cgcggtcagc gagctgcccg
     2161 aggcggacct gctcgccgcg ttgcgcgccg cagtcgccca ccagatgctc gtccccgacc
     2221 ccgccaccga gacctacgcc ttccgacacg cgctgaccca ggaggcgctg tacgccgagc
     2281 tgctgcccgg ggagcgcgcc cggctgcatg ccgccttcgc ccgtgtgctc accgagcgcc
     2341 ccgacctcgc cgagcccgac cggctcgccg tgccggctcg gctcgcctac cactgggtca
     2401 gggcccacga cccagctcgt gcgctgcccg ccgccgtgga ggcggggttg cagtccgagg
     2461 cggcctacgg gttcgctgac gcccagcgtc acttcgagat ggcgctcgag ctgtgggatc
     2521 aggtggctga cgccgaagag cggctcggcc tcgatcgtgc cgcggtgctg cagcacgcgg
     2581 ccgagtcggc ctacctggcc ggtgacccga accgagccat cacgctgacc cgggcggcgc
     2641 tcgtcgacgt ggacgcggcc aaggatccgg tgcgggccgg gctcctgcac gggcggctcg
     2701 gcggctacct gcgggcgacg ggcggcgagg gcgcgttcgc cgagtacgag gcggccgtgc
     2761 gcctggtgcc cgcgagcccg ccactggccg agcgtgcgca ggtcctggcc gccttcggcg
     2821 aggcactgat cgggcagggc cactaccgcg agtctcgcga cctgtgcgag gaggcgatcg
     2881 cgatcgcccg ccagatcggc gccctcgccg aggaggggga tgcccgcagg gctctggggg
     2941 tggacctcgc cttcctcggc gacctcgagg cgggcgtcgg gcagctcagc gaggcccgcc
     3001 ggatcgccga ggcggtcggc aaggtggacg aggtcgcgcg cgccttcgcc accctgtcgg
     3061 gcctgctgga agcgttcggc gagctggagg cggcaaccgc gctcgccttg gagggtgccg
     3121 agctggcggc cagccagggg cttgggcggt ggcacagccc attcctgacc gcgaccgccg
     3181 cgcgtgcgct gttcgcgctc ggtcgctggg acgacgccga cctgctgctg caccgcgcgg
     3241 ccgatcgcgt ggcccccgag ctcgccgcca cgcgcgtcta catccacacg gcccgcagcc
     3301 agctcgactt cgcccaaggt cttgccgcct cggccgccga gcacctggcg gtggcccgcg
     3361 aggcctacgc gcagaccgtc acgcagccct ggttcgccac gccgctgttc accgcaacgg
     3421 cggagctggc catgctggag ggccgcttga acgacgccga cgccgccgtg gccgaggggc
     3481 ttcgggtcgc cggtgcgaat ctcatcttcg gcgctccgct gtacgccctg gggttgcgcg
     3541 cggccgcgga tcgcgccgag ctggcaagcg cccgccgcac cggcgacgag gtcctcgagg
     3601 cccgccgggc cgggagcgca ctggccagcg agctacgcgc tcggatgtcg cccgagggag
     3661 ccgacggcac ggtgcccacg ccccgcaccg aggcccaggc ggtgctctgc gaggcggagc
     3721 tcgcccggct cggcgggcgc agcgaccctg acctgtgggc ggcagccgcc aatgtctggg
     3781 agcggctcgg cgagccctac ccgaccgcct acgcccgctg gcgcgaggcc gaggcactgc
     3841 tgctcggcgg cctcgtccgc gagcaggtcg agccgtcgct gcgcgccgcg cacgcgacgg
     3901 cgagcgagct tggcgcggcc ccgctgcgcg ccgagatcga ggccctggcc cgccgcggcc
     3961 gcctcaatct cgggaccgat cccgcgacca cccctccccc tgcacccgag ccgccatcgc
     4021 cgctcaaccg actcggtctc acggcccgcg agcaggaggt gctcgctctg gtcgcgatcg
     4081 ggcggacgaa ccgccagatc gccgagacgc tgtt
//
LOCUS       DASPYY010000198         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2284117, whole genome shotgun sequence.
ACCESSION   DASPYY010000198 DASPYY010000000
VERSION     DASPYY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284117"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>1788
                     /locus_tag="VEP73_03025"
     CDS             49..>1788
                     /locus_tag="VEP73_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25442.1"
                     /translation="MTARAPVAPRGTSAPAAQRVRGVVWAALAVAVAAPPVAYLAVER
                     RHRLLPLGWDIFGYIWQTGAVGHGALSALGTRPGMPAMAALLRAAIPLAPAREMVVLP
                     LALTVALAFACAAAVRTSFRLPPWTAPAVALVVALWPGSARILVGYEANLLLLVLVAA
                     GLTALIHAGGRTPSLVLAGAMFLAAALTHAAIFAAFSVVTALFVLLSGRAFRSDRRDG
                     VPLLGTDAGAAAVTVIGAGAVGAGVLFGALGLRPSQTINTSQVSFLFRDRTIEELRRA
                     RPPASVPVGFLGAAWSWAMGGTRSARALLRFGAAWLAVGAAGILLSLRGNGIPGARFL
                     LFALPVPVLAGLGVAGTAWLVAGRARWWSAGLGVAVVGVMASGFAWPGLQFVRYQFTP
                     LRSSTMAEQRGAFAYLTTVPGSPPVVFVVNQPGPHGAFTPKLRFNVLRSFVPPDQIDR
                     TFVYVGDYENLLAGRPTLIPPTQPWQVTYDTVSRDTWAQAGPAFREGAVVLITRNDDV
                     DGFGRAVASDPSREVATGLYALRGPILHVPTPPPGRPFGTAAAGLAVLGYLAALFALG
                     WGPAGVALRDTGAT"
BASE COUNT          156 a          674 c          680 g          278 t
ORIGIN      
        1 ggatcctcgc ggccccgggc ccgaagggga cgccccgagc gcgcccgcat gacggcgcgc
       61 gcgccggtcg ctccccgcgg cacgtcggct ccggccgccc agcgggtccg cggggtcgtg
      121 tgggccgcgc tcgcggtcgc cgtcgccgct ccgccggtgg cgtacctcgc cgtcgagcgc
      181 cgccaccgcc tcctgccgct cggctgggac atcttcggct acatctggca gaccggcgct
      241 gtgggccatg gcgcgctctc ggcgctcggg acccgcccgg ggatgccggc gatggcggcg
      301 ctgctccggg ccgcgatccc gctcgctccc gcccgcgaga tggtcgtcct gccgctcgcc
      361 ctcaccgtcg ccctggcgtt cgcgtgtgcg gccgcggtcc ggacctcgtt ccggctcccc
      421 ccgtggacgg cgcccgccgt ggccctggtg gtcgcgctgt ggccggggag tgcccgcatc
      481 ctcgtcggct acgaggcgaa cctcctcctt ctcgtcctgg tggcagcggg actgacggcg
      541 ctcatccacg cgggcggtcg aaccccgtcg ctcgtgctgg ccggcgcgat gttcctcgcg
      601 gccgccctca cacacgcggc catcttcgcc gccttctccg ttgtcaccgc gctgttcgtg
      661 ctgctgtccg gccgggcgtt ccggagcgac cggcgggatg gcgtgccgct cctcggcacc
      721 gacgccggcg ccgccgcggt caccgtgatc ggcgccggag ccgtgggcgc cggggtgctg
      781 ttcggagcgt tggggctccg gccatcccag acgatcaaca cgtcgcaggt gagcttcctg
      841 ttccgggacc ggacgatcga ggagctgcgc cgcgcccgcc cgccggcaag cgtgccggtc
      901 ggcttcctgg gtgcggcctg gtcctgggcg atgggcggga cgcggagcgc ccgcgcgttg
      961 ctgcggttcg gtgcggcgtg gctcgcggtg ggggccgccg ggatcctgct gtccttgcga
     1021 gggaacggca tccccggagc ccgtttcctc ctcttcgccc tgccggtacc ggtgctggcg
     1081 gggctcggcg tggccgggac ggcgtggctg gtggccgggc gagcccgctg gtggagcgcc
     1141 gggctcggcg tggccgtcgt cggcgtgatg gcctccgggt tcgcctggcc cggcctgcag
     1201 ttcgtgcgct accagttcac cccactccgg tcctcgacga tggcggagca gcgaggtgcg
     1261 ttcgcctacc tgacgaccgt gcccggctcg ccgccggtgg tgttcgtggt gaaccagccg
     1321 gggccgcacg gggcgttcac gccgaagctc cggttcaacg tgctgcgctc gttcgtcccg
     1381 ccggaccaga tcgaccggac gttcgtctac gtgggggatt acgagaacct gctggccggg
     1441 cggccgacct tgatcccgcc gacccagccg tggcaggtga cgtacgacac ggtctcccgg
     1501 gacacctggg cgcaggcggg acccgcgttc cgggaggggg cggtcgtcct catcacccgg
     1561 aacgacgacg tcgacgggtt cgggcgggcc gtcgcctccg acccctcccg cgaggtggcc
     1621 acggggctct acgcgctgcg gggaccgatc ctgcacgtgc ccacgccgcc gcccgggcga
     1681 ccgttcggca ccgcggcggc gggcctcgcg gtcctggggt acctggcggc gctgttcgcg
     1741 ctgggatggg gaccggccgg cgtggcgctc cgggataccg gggcgacc
//
LOCUS       DASPYY010000199         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2284177, whole genome shotgun sequence.
ACCESSION   DASPYY010000199 DASPYY010000000
VERSION     DASPYY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284177"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /gene="mfd"
                     /locus_tag="VEP73_03030"
     CDS             <1..1801
                     /gene="mfd"
                     /locus_tag="VEP73_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HYT25443.1"
                     /translation="VRALAFDLLRLYAQREVAPGIAVGPDTVWQEEMEAAFPYIETPD
                     QAQAVEDVKGDMEQSRPMDRLVCGDVGYGKTEVAMRAAFKAVMDGKQVAILVPTTLLA
                     QQHHATFTERLAPFPVKVGTLSRFQSRQEQEETVAAMAAGTVDVVIGTHRLLSSDAKW
                     SDLGLVVVDEEQRFGVTHKEHLKQLRTEVDVLTLTATPIPRTLEMSISGIRDMSVMET
                     PPEERHPVLTFVGPYDEATVANAIRREMLREGQVFFVHNRVDTIDRLALRVRQLVPEA
                     RVGVAHGQMSEEQLERVMLDFWDKQYDVLVCTTIIESGLDIPTANTLVVDRADTLGLA
                     QLYQLRGRVGRSRERAYAYLFYPPERTITESSHARLSAVATHQDLGSGMAIAMKDLEI
                     RGAGNLLGADQSGHVALVGYDLYMQMLAEAVAEMRGRPIEQPKELKLEVPVDAHLPAG
                     YVQRERLRLEAYRRLGGARSVDEVAALGAELADRYGPPPPPVRNLLALAGVRAQATAL
                     GLTEVVCFGGRVRLAPVPALPESKQVRLDRLYRGAVWKAAEETLVVPLPPAGAGDVDL
                     PAWLSELLEGVLGAPPPPGLPPAGHREPDRAAS"
     gene            complement(1824..1991)
                     /locus_tag="VEP73_03035"
     CDS             complement(1824..1991)
                     /locus_tag="VEP73_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25444.1"
                     /translation="MSADTVILREALAGLPAAERPAFVHAVAERLPGAREGRPEIDYV
                     RLNVTARRGSA"
     gene            2102..3184
                     /locus_tag="VEP73_03040"
     CDS             2102..3184
                     /locus_tag="VEP73_03040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYT25445.1"
                     /translation="MRRLLPTIVKPVAPVAVVLVAVALAAAGCGSGSKARTVSVDQAA
                     TVDGVGIPVARLTELAKVSSGQGTQQPGSAETTRSALESLIQSQVVLGGARAEGVTVS
                     DTDVASRLNDLKAQVASQGGNFDEALRQRNLTVPILENQLRVQLAAEKVAAKLVPGQS
                     DAKLLEALGKRKDEFLQLHVRHVLVKDEATAKKVRGLLVDKGDWAGVAKRYSTDPGSK
                     DKGGDLGFESKGQTVAEFEKATFKLAGEGDCKGKTGGACTSPISDPVKSQYGWHVIQV
                     TGVQLPQLNAQLRDQLEPGLSKQRQDAIQAWFSKHLKAAKVTVNPRFGRWDAATGKVV
                     DRETAPRTTPETTAGKQGSATTTTQP"
BASE COUNT          481 a         1221 c         1110 g          403 t
ORIGIN      
        1 cgtccgcgct ctcgcctttg acctactgag gctgtacgcg caacgggagg tcgcgccggg
       61 gatcgccgtc ggcccggaca cggtgtggca agaggagatg gaggctgctt ttccctatat
      121 cgaaacaccc gaccaagccc aagccgtgga ggacgtgaag ggggacatgg agcaaagccg
      181 cccaatggac cgcctcgtct gcggcgacgt cggctacggg aagaccgaag tcgcgatgcg
      241 cgccgccttc aaggcggtca tggacggcaa gcaggtcgcc atcctggtcc ccaccacgct
      301 gctggcccag cagcaccacg ccaccttcac cgagcggctc gcgcccttcc cggtcaaggt
      361 cgggacgctc tcccgcttcc agtccaggca ggagcaggag gagacggtcg ccgcgatggc
      421 cgccggcacc gtcgacgtcg tcatcggcac ccaccgcctg ctgtcgtccg acgcgaaatg
      481 gtccgacctc ggcctcgtcg tggtcgacga ggagcagcgc ttcggcgtca cccacaagga
      541 gcacctgaag cagctccgca ccgaggtcga cgtgctgacc ctgaccgcca ccccgatccc
      601 gcggaccctc gagatgagca tctccgggat ccgcgacatg tcggtgatgg agaccccgcc
      661 ggaggagcgc cacccggtgc tcacgttcgt cggcccctac gacgaggcga ccgtcgccaa
      721 cgccatccgg cgggagatgc tccgcgaggg ccaggtcttc ttcgtccaca accgggtcga
      781 cacgatcgac cggctcgcgc tgcgggtgcg ccagctggtg cccgaggcgc gggtcggcgt
      841 cgcccacggc cagatgagcg aggagcagct cgagcgggtc atgctcgatt tctgggacaa
      901 gcagtacgac gtgctggtct gcacgacgat catcgagtcc ggcctcgaca tcccgaccgc
      961 caacacgctg gtcgtggacc gggccgacac cctggggctg gcgcagctct accagctcag
     1021 gggccgggtc ggccgctccc gcgagcgcgc ctacgcctac ctgttctacc cgccggagcg
     1081 caccatcacc gagagctcgc acgctcgcct gtccgccgtc gccacccacc aggacctcgg
     1141 gtcgggcatg gccatcgcca tgaaggacct cgagatccgc ggcgccggca acctcctcgg
     1201 cgccgaccag tccggccacg tcgcgctcgt cggctacgac ctctacatgc agatgctcgc
     1261 cgaggcggtc gccgagatgc ggggacgccc gatcgagcag ccgaaggagc tcaagctcga
     1321 ggtcccggtc gacgcgcacc tgcccgccgg ctacgtccag cgcgagcggc tccgcctgga
     1381 ggcgtaccgg cggctcggcg gggccaggtc ggtcgacgag gtggccgcgc tcggggccga
     1441 gctggccgac cgctacggcc ccccgccgcc cccggtgcgc aacctcctcg cgctcgcggg
     1501 cgtccgcgcc caggccacgg ccctggggct caccgaggtc gtctgcttcg gcggccgcgt
     1561 ccggctcgcg ccggtgccag cgctccccga gtccaagcag gtccgcctcg accgtctcta
     1621 ccggggcgcg gtgtggaagg cggccgagga gaccctcgtc gtgcccctgc cccccgcggg
     1681 cgcgggcgac gtggacctgc cggcctggct gtccgagctg ctcgagggcg tcctcggcgc
     1741 cccacccccg cccgggctgc cgcccgccgg ccaccgggag cccgaccgcg ccgcctcctg
     1801 agccgtcggc tccggccttc tcctcaggcg gagccccggc gggcggtgac gttcagccgc
     1861 acgtagtcga tctcgggccg cccctcgcgc gccccgggca gccgctcggc gaccgcgtgc
     1921 acgaaggccg gccgctcggc ggccggcagg ccggccagcg cctcccggag gatcacggtg
     1981 tcggcgctca tcgggccagt ctgccagcga tccccgtggg ccggccggcg gtccccgccg
     2041 gccggcgggt gcgactatcc ttgaccgtcg tcatccgctg aaccagaacg cgaggtcgtc
     2101 cgtgcgtcgt ctcctgccca ccatcgtcaa gcctgtcgcg ccggtcgccg tcgtgctggt
     2161 cgccgtcgcg ctggccgccg ccggctgcgg ctccgggtcc aaggccagga ccgtctcggt
     2221 cgaccaggcc gccacggtcg acggggtcgg catccccgtc gcgcggctca ccgagctcgc
     2281 caaggtgagc agcggccagg gcacccagca gcccgggagc gccgagacca cccgctcggc
     2341 cctcgagagc ctgatccagt cccaggtcgt cctcggcggg gccagggccg agggcgtgac
     2401 cgtctccgac accgacgtcg ccagccgcct gaacgacctc aaggcgcagg tcgcgtccca
     2461 gggcggcaac ttcgacgagg cgctgcggca gcgcaacctg accgtgccga tcctcgagaa
     2521 ccagctccgg gtccagctcg ccgccgagaa ggtggcggcc aagctggtgc ccggccagag
     2581 cgacgccaag ctgctcgagg ccctcggcaa gcgcaaggac gagttcctgc agctccacgt
     2641 caggcacgtg ctcgtcaagg acgaggcgac cgccaagaag gtccgcggcc tgctcgtgga
     2701 caagggcgac tgggcgggcg tggccaagcg gtactccacc gacccgggca gcaaggacaa
     2761 gggcggcgac ctcggcttcg agagcaaggg ccagaccgtc gccgagttcg agaaggccac
     2821 cttcaagctg gccggcgagg gcgactgcaa ggggaagacc gggggggcgt gcacctcgcc
     2881 gatctccgac ccggtcaagt cccagtacgg ctggcacgtc atccaggtga ccggggtcca
     2941 gctcccgcag ctcaacgcgc agctgcggga ccagctcgag cccggcctgt ccaagcagcg
     3001 gcaggacgcg atccaggcgt ggttctcgaa gcacctcaag gcggccaagg tgacggtcaa
     3061 cccgcgcttc ggccgctggg acgccgcgac gggcaaggtg gtcgaccggg agaccgctcc
     3121 gcggaccacc ccggagacca cggccggcaa gcagggatcc gcgaccacca ccacgcagcc
     3181 gtagcacggc catggccagg gtcgtcctcg tcgcg
//
LOCUS       DASPYY010000200         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_878964, whole genome shotgun sequence.
ACCESSION   DASPYY010000200 DASPYY010000000
VERSION     DASPYY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878964"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VEP73_03045"
     CDS             <1..932
                     /locus_tag="VEP73_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYT25446.1"
                     /translation="VVVGAIVLGARWVARHPERVRALVDRQLDRPWVRRLRVRYRRQL
                     GFLARRLRPGGALGLSLTITAAGLAGAGWAFGAVLQDVLARDEAALVDGPVWRFFVAH
                     REAWLTPLMRGVTDLGNAAVLIGLIGVAGLAWWWRRRTRRPLGLLAGAYLGAWVLSEA
                     IKDLTRRPRPPTAQAIGHWTGYAFPSGHTSKATAVYGMLAALLAASTSRWSSKVAWWT
                     AAVLLAGLVGLSRLYLGANWLTDVLGGLALGAAWLFGLLTATRTVHALRAGAGDDRPA
                     PPAPRPPRESERPPEGGMPGDPGQPARRHRGSG"
     gene            complement(961..1431)
                     /locus_tag="VEP73_03050"
     CDS             complement(961..1431)
                     /locus_tag="VEP73_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYT25447.1"
                     /translation="MPTPEERLEELGITLPEPAAPLAAYVPCVRTGDLVYVSGQVPLV
                     DGKPGWTGRLGASELTVEDGAQAARRCAVNVVAALKRELGDLSRVRRVVKVTGYVASD
                     PGFTEQPKVVNGASELLAEVFGAAGRHARAAVGVAALPMGVPVEVEAIVEVADG"
BASE COUNT          157 a          583 c          581 g          195 t
ORIGIN      
        1 tcgtggttgt cggggccatc gtgctcgggg cgcgctgggt cgcccgccac cccgagcggg
       61 tccgcgcgct ggtcgaccgg cagcttgacc gcccctgggt cagacggcta cgggtgcgct
      121 accggcggca gctcgggttc ctggcccgcc ggctgcgccc cgggggggcg ctcggcctgt
      181 cgctcaccat cacggcggct ggcctcgccg gggcgggctg ggcgttcggt gcggtgctcc
      241 aagacgtcct cgcccgcgac gaggccgccc tcgtcgacgg gccggtctgg cgcttcttcg
      301 tcgcacaccg ggaggcgtgg ctcaccccgc tgatgcgcgg ggtgaccgac ctgggcaacg
      361 cggccgtgct gatcgggctg atcggggtcg ccgggctggc ctggtggtgg cgccggcgca
      421 cgcggcggcc gcttggcctg ctggccggcg cctacctggg cgcctgggtg ctgtcggaag
      481 cgatcaagga cctgacccgc cggccccgcc cgcccaccgc ccaggcgatc ggccactgga
      541 ccggctacgc cttcccgtcc gggcacacca gcaaggccac cgcggtgtac ggcatgctcg
      601 ccgcgctgct cgccgcgagc acctctcgct ggagcagcaa ggtcgcctgg tggaccgccg
      661 cggtgctgct cgccgggctg gtcgggctgt cccggctgta cctgggcgcg aactggctca
      721 ccgatgtgct cggcgggctc gcgctcggcg cggcctggct gttcggcctg ctcaccgcca
      781 cccgcaccgt ccacgcgctc cgcgcggggg ccggcgacga ccgccccgcc ccacccgctc
      841 cccgcccgcc aagggagagc gagcgcccgc ccgagggcgg catgccgggc gacccggggc
      901 aacccgcgcg gcgccaccgg gggagcgggt gatggtcggg ctcgaccgct cggccgccgg
      961 ctacccgtcg gcgacctcga cgatggcctc cacctcgaca ggaacgccca tggggagggc
     1021 ggccacgccg acggcggccc tggcgtggcg gccggcggcg ccgaacacct cggcgagcag
     1081 ctcgctcgcc ccgttgacga ccttgggctg ctcggtgaag ccggggtcgc tcgcgacgta
     1141 cccggtcacc ttcaccaccc gacggacccg ggagaggtcg ccgagctccc gcttgagggc
     1201 ggcgacgacg ttgacggcgc agcgcctggc ggcctgggcg ccgtcctcga cggtgagctc
     1261 gctcgcgccg agccggcccg tccagcccgg cttgccgtcc accagcggca cctgcccgga
     1321 gacgtacacg aggtcgccgg tgcggacgca gggcacgtag gcggcgaggg gcgccgccgg
     1381 ctcgggcagc gtgatgccga gctcctcgag gcgctcttca ggggttggca tggcggctcc
     1441 ttgcggcgtc ggctcgtcgg gcgggggcaa gcctaccgcc gtccgccggt agcgaagaac
     1501 gcgcgcacgt cggcca
//
LOCUS       DASPYY010000201         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2003175, whole genome shotgun sequence.
ACCESSION   DASPYY010000201 DASPYY010000000
VERSION     DASPYY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003175"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..439
                     /locus_tag="VEP73_03055"
                     /pseudo
     CDS             1..439
                     /locus_tag="VEP73_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673769.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketosynthase chain-length factor"
     gene            452..721
                     /locus_tag="VEP73_03060"
     CDS             452..721
                     /locus_tag="VEP73_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYT25448.1"
                     /translation="MSVTVEDIRRILIACSGAPELEVPDVDFADTSLEDLGYDSLALM
                     ESAAAIEQEYGVKISDDTFGAAKTPRELTRIVESEIAGLDTKAAA"
     gene            718..1659
                     /locus_tag="VEP73_03065"
     CDS             718..1659
                     /locus_tag="VEP73_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatase/cyclase"
                     /protein_id="HYT25449.1"
                     /translation="MTQGVIASEIRRMEHFRVVPAPAERLYDLVADVSRWPVVLGPCV
                     AARRLELIGDEERIEISADVNGEVKTWTSRRTLDSAGLRVAFRQELSAPPVATMSGEW
                     SFAPGDDGRTRMTLTHAFSALTGDRKTLNWIAAAVDRNSEQELAALGELAALGHPLEE
                     LVLSFADRVELPGTAVADAYEFVARAELWPQRLPHVRRVDLDESVPGVRRLEMETVTA
                     DGGSHTTQSVRLCSPGARIAYKQTVRPQLLSGHSGVWEFEALDGGTAITSRHTVAIDP
                     AAVVAVLGAGTTLAQAGAWARERLGANSRATMEAAGA"
     gene            1656..3191
                     /locus_tag="VEP73_03070"
     CDS             1656..3191
                     /locus_tag="VEP73_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT25450.1"
                     /translation="MKLADIAAAQGTDPDRVCMVAAGRSFTRRQVRNEVARCRAELLE
                     RGVAPGERVLALLDHGPDAAFFLAAASALGLHLLMPYGLQAAALPEWLSIMDSARPDV
                     IVHAKRDRTGVDALRARGGRVVELLPPRGDASDAELRIDHPAPVEQFLMLFTSGTTGR
                     PKAISIGETQAGGRMAAVTRTMGYRPDARIFMTGLMNNTTGVINSFGALLHGATVVFP
                     ADPDPARWPAQVAAERATHLALRPVALKRFVAAVDAERPDLSSLRVVSYGGAAVPGAV
                     LERGRALIGADWIQGYGLTETFGPFCFLDEVGHAEQRHVKHIYCIGRPDDTLDVQVEP
                     VAEHPDGVGEVLVRGALVMEGYVDVATGRIEPVGEWLRTGDLAEWSPDGDLVLKGRRS
                     GVLLSENGHRIYPEEVEAVLAGAAGVDDVVLVGLPGSGTPHERPVACLSGPIGAREPG
                     VVRAIVAGELEAGLALEKWPDLAYATAQPFPKSANGKIMRGAVAAGIDPAALIELGGD
                     GRG"
     gene            3184..4335
                     /locus_tag="VEP73_03075"
     CDS             3184..4335
                     /locus_tag="VEP73_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT25451.1"
                     /translation="MADDVTRLRVPEHVYRALGYLFPRLFFLNARYAPQVHWGDVAAA
                     LDGFPPDDLDLSSAVFWNAWRERWTAQADGYVRLAEASTTAAGRSRALRGAAAAYHWA
                     EFQYFDDPALKRHLRQRIRDCFLGSLEGTDLELTDGRLPARPGRPDVPYWLLTTAAMR
                     AEGWPLPSVLLCNGLDSATEVEVLALAEAYLERGIAAVLFEGPGQGMQLGRAPLHTTM
                     EEVVADLVVHLAAEPAIAADRLAFAGISFGGHIALRVARALGDRFRCVVNFGGGPALN
                     KFDGLPRRLKANFRFALAAGADADLQPVFDDLALEPGPAPATDVLSVHGALDDIFPVE
                     GLADVDRAWAGRHRLVVHPREAHTSLNLINAYTLETADWVAGRLGRSPG"
     gene            4332..>4513
                     /locus_tag="VEP73_03080"
     CDS             4332..>4513
                     /locus_tag="VEP73_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25452.1"
                     /translation="MNLLVQAGLLGDAPLQCDERRRLLWAQAAEQLGLELGDQGLGLG
                     VQRPAGRQQMHTEAEAL"
BASE COUNT          552 a         1643 c         1720 g          598 t
ORIGIN      
        1 gaccccgcgc ccggctcgga ccggccgccg gcgctgcgcc ggggcgatcg aggcggcgct
       61 ggccgacgcg ggcctcgccg ccgccgacgt cgacgtcgtg ttcgccgacg cggtcggcgt
      121 ccccgagctg gaccggaccg aggcggcggc gctgcgggcg gtgttcgggc ccggcggcgt
      181 gccggtcacc gccccgaagg ccgcgtccgg ccggacctac ggcggcggcg ggtcgctcga
      241 cgtcgccgcc gcgctgctgg cgatccgcga cggcgtcatc ccgccgaccg ccggcaccgg
      301 cgacgtgccg gccgagtacg gcctggacct ggtctgcggc gccgcccgcg acgcggaggt
      361 gcgcacggcg ctcgtcctcg cgcggggtct gtggggcttc aactcggccg tcgtcgtccg
      421 taccgcatcc acttcctagg gaggaaccac catgtccgtc accgtcgaag acattcgccg
      481 catcctcatc gcctgctccg gcgcgccgga gctcgaggtg cccgacgtcg acttcgccga
      541 caccagcctc gaggacctcg gctacgactc gctcgcgctg atggagtcgg ccgccgcgat
      601 cgagcaggag tacggggtga agatctcgga cgacacgttc ggggccgcca agaccccccg
      661 cgagctgacc aggatcgtcg agtcggagat cgccggcttg gacacgaagg cggcggcatg
      721 acccagggag tcatcgcctc ggagatccgg cggatggagc acttccgggt cgtgccggcg
      781 ccggcggaac ggctgtacga cctcgtcgcc gacgtgtccc gctggccggt cgtcctcggg
      841 ccgtgcgtcg ccgcgcgtcg cctggagctc atcggcgacg aggagcgcat cgagatctcg
      901 gccgacgtca acggcgaggt caagacgtgg acgtcgcgcc gcacgctgga ctcggccggc
      961 ctgcgggtcg cgttccggca ggagctcagc gcgccgccgg tcgccacgat gtccggcgag
     1021 tggtccttcg cgccggggga cgacgggcgg acgcggatga cgttgacgca cgccttctcg
     1081 gcgctcaccg gcgaccgcaa gacgctgaac tggatcgccg cggcggtcga ccgcaacagc
     1141 gagcaggagc tcgccgcgct cggcgagctc gccgcgctcg ggcaccccct cgaggagctg
     1201 gtcctgagtt tcgccgaccg ggtcgaactg ccgggcaccg cggtcgcgga cgcctatgag
     1261 ttcgtcgccc gcgccgagct ctggccgcag cggctgccgc acgtgcggcg ggtggacctc
     1321 gacgagagcg tgcccggagt gcggcggctg gagatggaga ccgtcacggc cgacggcggc
     1381 tcgcacacca cgcagtcagt ccggctctgc tcgccgggcg cccggatcgc ctacaagcag
     1441 acggtgcgtc cgcagctgct gtccggccac agcggcgtgt gggagttcga ggcactcgac
     1501 ggcggcacgg cgatcacctc gcggcacacg gtggcgattg acccggcggc ggtggtcgcg
     1561 gtccttggcg ccggcacgac gctggcgcag gccggcgcct gggcgcgcga acggctcggc
     1621 gccaacagcc gcgccacgat ggaggcggcg ggcgcatgaa gctggcggac atcgccgcgg
     1681 ctcagggcac cgacccggac cgggtgtgca tggtcgcggc cgggcgctcg ttcacccggc
     1741 ggcaggtgcg caacgaggtg gcccgttgcc gcgcggagct cctcgaacgc ggcgtcgcac
     1801 cgggcgagcg ggtcttggcg ctgctggacc acggcccgga cgctgcgttc ttcctggccg
     1861 cggcgagcgc gctcggcctg cacctgctga tgccgtacgg cctgcaggcg gcggccctgc
     1921 ccgagtggct gtccatcatg gacagtgcgc ggcccgacgt gatcgtgcac gccaagcgcg
     1981 accgcaccgg cgtcgacgcg ctccgtgcgc gcggcgggcg ggtcgtcgag ctgctcccgc
     2041 cgcggggcga cgcttcggac gccgagctgc gcatcgacca ccccgcgccg gtggagcagt
     2101 tcctgatgct gttcaccagc ggcaccaccg gccggccgaa ggcgatcagc atcggcgaga
     2161 cacaggcggg cgggcggatg gcggcggtca cccgcacgat ggggtaccgc ccggacgccc
     2221 gcatcttcat gacgggcctg atgaacaaca cgaccggcgt catcaactcg ttcggggcgc
     2281 tgctgcacgg cgccaccgtc gtcttccccg ccgacccgga cccggcgcgg tggccggccc
     2341 aggtcgcggc cgagcgcgcc acgcacctgg cgctgcggcc ggtggccctc aagcggtttg
     2401 tggcggcggt cgacgccgag cggcccgacc tgtcgagcct gcgggtcgtg tcctacggcg
     2461 gggccgccgt gccgggtgcg gtcctggagc ggggccgggc gctcatcggc gccgactgga
     2521 tccagggtta cggcctcacc gagaccttcg ggccgttctg cttcctggac gaggtgggcc
     2581 acgccgagca gcgccacgtc aagcacatct actgcatcgg ccgccccgac gacaccctcg
     2641 acgtgcaggt cgagccggtg gccgagcatc cggacggcgt cggcgaggtg ctggtccggg
     2701 gcgcactggt gatggagggg tacgtcgacg tcgcgaccgg ccggatcgag cccgtcggcg
     2761 agtggctgcg caccggcgac ctcgccgagt ggagcccgga cggcgacctg gtgcttaagg
     2821 gccgccggtc gggcgtgctg ttgagcgaga acggccatcg catctacccc gaggaggtcg
     2881 aggcggtcct ggccggcgcg gcgggcgtcg acgacgtggt gctcgtgggc ctgcccggct
     2941 cgggcacccc gcacgagcgg ccggtggcgt gcctgtccgg gccgatcggc gcgcgggagc
     3001 ccggcgtggt gcgcgcgatc gtggccggcg agctcgaggc gggtctggcg ctggagaagt
     3061 ggccggacct cgcgtacgcg acggcgcagc cgttcccgaa gagcgccaac ggcaagatca
     3121 tgcggggcgc ggtggccgcg ggcatcgacc cggccgcact catcgagctg ggtggtgacg
     3181 gtcgtggctg atgacgtgac ccgcctgcgg gtgcccgagc acgtctaccg ggccctgggg
     3241 tacctcttcc cgcgactctt cttcctcaac gcgcgctacg cgccgcaggt gcactggggc
     3301 gacgtcgccg ccgccctgga cggcttcccg cccgacgatc tcgacctgag ctcggcggtg
     3361 ttctggaacg cctggcggga gcggtggacg gcccaggccg acggctacgt gcgcctcgcc
     3421 gaggcctcga ccacggcggc ggggcgttcc cgggccctgc gcggcgcggc ggcggcctac
     3481 cactgggcgg agtttcagta cttcgacgac ccggccctca agcggcacct gcggcagcgg
     3541 atccgcgact gcttcctggg cagcctggag ggcaccgacc tcgagttgac cgacggccgg
     3601 ctgcccgcgc ggcccggccg tcccgacgtg ccctactggc tgctgacgac cgcggcgatg
     3661 cgcgccgagg ggtggccgct gccgtcggtg ctgctgtgca acggcctgga ctcggcgacc
     3721 gaggtcgagg tcctcgccct ggccgaggcg tacctcgagc gcggcatcgc cgccgtgctg
     3781 ttcgagggcc cgggccaggg catgcagctg ggccgggcgc cgctgcacac cacgatggag
     3841 gaggtcgtcg ccgacctcgt ggtgcacctg gcggccgagc ccgcgatcgc ggccgaccgg
     3901 ctcgccttcg ccggcatcag cttcggcggc cacatcgcgc tgcgggtggc gcgggcgctc
     3961 ggcgaccggt tccggtgcgt cgtgaacttc ggcggcggcc cggcactgaa caagttcgac
     4021 ggcctgccgc gccggctgaa ggccaacttc cggttcgcgc tcgccgccgg cgccgacgcg
     4081 gacctgcagc ccgtcttcga cgacctcgcc ctcgagccgg gtcccgcgcc ggcgacggac
     4141 gtgctgagcg tccacggcgc gctcgacgac atctttccgg tcgagggcct ggccgacgtg
     4201 gaccgggcgt gggccggccg gcaccggctc gtcgtgcacc cgcgcgaggc gcacacgagc
     4261 ctcaacctga tcaacgctta cacgctggag accgcggact gggtcgccgg tcggctgggc
     4321 cgcagcccgg ggtgaacctc ctggtgcagg ccggcctgct cggagacgcg ccgctgcagt
     4381 gcgacgaacg tcgccgcctc ctgtgggctc aggcagccga gcagcttggt ctcgaactcg
     4441 gtgatcaggg cctcggcctc ggcgtgcagc gcccggccgg gcggcagcag atgcacaccg
     4501 aggccgaggc cct
//
LOCUS       DASPYY010000202         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1021338, whole genome shotgun sequence.
ACCESSION   DASPYY010000202 DASPYY010000000
VERSION     DASPYY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021338"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VEP73_03085"
     CDS             <1..656
                     /locus_tag="VEP73_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25453.1"
                     /translation="VAALLGALNEVPVTPGSAVLVPAGVPHAIGEGVLLVELQEPTDF
                     SVLLEWEGFAVDGAVAGHLGLGFDLALECVDRSGWGRERLAELHGRRAADPAGRPGVE
                     PLLPEAADPFFRAERIRPRSPATLEPSFSVLVVLDGSGRLDTAAGGRLDLARGDTVLV
                     PFAAGPGLVSGAVHAIRCLPPLPGQAAGQGAPARTEGAAPHGRGGGAAPPGPDGGAG"
     gene            653..2032
                     /locus_tag="VEP73_03090"
     CDS             653..2032
                     /locus_tag="VEP73_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYT25454.1"
                     /translation="MTAELLVGIDVGTTSCKAAVVAPDGAELAHGRAPTPWVPVATGA
                     EIAPGALVDSALAAAAGALRDHRDARVVAVGVTSMGETGALLGRDGEPVAPAIAWHDA
                     RGGAAAEELAAALGGERFTARVGLPPSRLCTLAKYRWLRDEAEGRRGVRWLNVAEWVV
                     HRLGGEQLAELSLASRTGLLDLATRAWWDEALDWAGAPTGLLPEPAVAGTPAGRVAAG
                     AAGPAAMLEGAVLAVAGHDHLCAAVGAGAVGPGEVFDSCGTAEALVRAVSPPVAPDRI
                     AAAVAGHVTVGWHVVGEFQALLGAQRAGFALQRFLDLLGVGPDRLAALEAAALAVPAG
                     TALPTVLDADGEQATLAGIGRHPSPAHVWRAAVEAVGARSGELLATVRAVAGDVDRLV
                     VAGGWSRSPTLRSVKARRLGRAVYPPVTEAGARGAALLAGIAAGLWEGVEDLPPFADR
                     RGPAAPGEE"
     gene            2035..2985
                     /locus_tag="VEP73_03095"
     CDS             2035..2985
                     /locus_tag="VEP73_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25455.1"
                     /translation="MPTPPSTNPPGTRPGLEALATAEGRFAVLAIDQRGSIVAGLGRP
                     GRPGAPADVTRFKLEACRALAPGASAVLLDPDYGLAPALEAGVVPDGVGVLVTQETEA
                     PRDVGGGRLSARHQERDAAFVARSGGQAAKFMVWLRPDRPRGAGEPDLVAETVAAVRE
                     VVADCAAAGLPSVVEALSYPLPGEAPLDPGAKAAVVLESARLLAATGPALLKLEYPGG
                     PAACRRVTDTVGMPWAVLSAGVGFDEFLGNLRVAMDGGASGFIAGRAIWKDAMGLDGQ
                     ERTAFLGDVARRRLDTCVRAMEGRGRSFAEAGRAMGRGGG"
BASE COUNT          277 a         1204 c         1205 g          356 t
ORIGIN      
        1 aggtagccgc gctcctcggc gccctcaacg aggtcccggt cacaccgggc tcggcggtgc
       61 tggtcccggc gggcgtcccc catgccatcg gcgagggcgt gctcctggtc gagctgcagg
      121 agcccaccga cttctccgtc ctgctcgagt gggaggggtt cgccgtcgac ggcgcggtag
      181 ccggccacct gggcctcggc ttcgacctcg cgctcgagtg cgtcgaccgc tccggctggg
      241 ggcgggagcg gctcgcggag ctgcacgggc ggcgcgccgc cgacccggcc ggccggccgg
      301 gcgtcgagcc gctcctgccc gaggcggccg accccttctt ccgcgccgag cgcatccggc
      361 cacgctcccc ggccacgctc gagccctcgt tctcggtcct ggtcgtgctc gacgggtcgg
      421 gccggctcga caccgccgcc ggcgggcggc tcgacctcgc ccgcggcgac acggtgctcg
      481 tccccttcgc ggccggcccc gggctggtct ccggggcggt ccacgccatc cgctgcctgc
      541 cgcccctgcc cggccaggcg gccgggcagg gcgctcccgc ccgcacggaa ggcgccgcgc
      601 cccacgggcg gggcggaggc gccgcgcctc cgggaccgga cggaggtgcc gggtgaccgc
      661 cgagctgctc gtcgggatcg acgtcgggac cacctcctgc aaggcggcgg tggtggcgcc
      721 ggacggggcc gagctggcgc acggcagggc gccgacgccg tgggtgccgg tcgcgaccgg
      781 ggccgagatc gcgcccggcg cgctggtgga ctcggccctc gccgcggcgg cgggcgccct
      841 ccgggaccac cgggacgcca gggtggtggc cgtcggcgtg accagcatgg gggagaccgg
      901 ggcgctgctc gggcgggacg gcgagccggt cgcgccggcg atcgcctggc acgacgcccg
      961 cggcggcgcg gcggccgagg agctggccgc cgcgctcggc ggcgagcgct tcaccgcccg
     1021 ggtcggcctg cccccgagcc gcctgtgcac ccttgccaag taccggtggc tcagggacga
     1081 ggccgagggc cgccgcgggg tccgctggct caacgtcgcc gagtgggtcg tccaccggct
     1141 cggcggcgag cagctcgccg agctgtcgct cgcgtcccgt accgggctgc tcgacctcgc
     1201 cacccgggcg tggtgggacg aggcgctcga ctgggccggg gccccgaccg gcctgctgcc
     1261 cgagccggcc gtggccggca cgccggctgg ccgggtggcc gccggggcgg ccggcccggc
     1321 cgccatgctc gagggcgccg tgctcgcggt cgccggccac gaccacctgt gcgcggcggt
     1381 gggcgccggc gcggtcggcc ctggcgaggt gttcgactcc tgcggcaccg ccgaggcact
     1441 cgtccgggcc gtctccccac cggtcgcgcc cgaccgcatc gccgccgcgg tcgccgggca
     1501 cgtcaccgtc ggctggcacg tcgtcgggga gttccaggcc ctgctcgggg cgcagcgggc
     1561 cgggttcgcc ctgcagcgct tcctcgatct gctcggggtc gggcccgacc gcctggccgc
     1621 cctggaggcg gccgctctcg cggtgccggc cgggaccgcg ctgcccaccg tcctcgacgc
     1681 cgacggcgag caggccaccc tggccggcat cggccgccat ccctctcccg cccacgtctg
     1741 gcgggccgcg gtcgaggcgg tcggcgcccg gtccggcgag ctgctcgcca ccgtccgggc
     1801 cgtcgccggc gacgtcgacc ggctggtggt cgcgggcggc tggtcccgca gcccgaccct
     1861 gcgctcggtg aaggcgcgcc ggctcggccg ggccgtctac ccgccggtca ccgaggccgg
     1921 cgcccgtggc gccgccctgc tcgccgggat cgcggccggt ctctgggagg gcgtcgagga
     1981 cctgccaccg ttcgcggacc gccgcggccc ggccgcgccg ggagaggagt gaccgtgcca
     2041 accccaccat ccaccaaccc gccgggcacc cgtcccggcc tcgaggccct cgccaccgcc
     2101 gagggccgct tcgccgtgct cgccatcgac cagcgcggct cgatcgtcgc cggcctcggc
     2161 cggccgggcc gcccgggcgc cccggcggac gtgacccgct tcaagctcga ggcctgccgt
     2221 gcgctcgccc cgggtgccag cgccgtgctg ctcgaccccg actacggcct ggcgcccgcg
     2281 ctcgaggccg gggtggtgcc cgacggggtc ggggtgctcg tcacccagga gaccgaggcc
     2341 ccgcgcgacg tcggcggtgg ccgcctgtca gcgcgccacc aggagcggga cgccgccttc
     2401 gtggcgcgct cgggcgggca ggcggccaag ttcatggtct ggctgcggcc cgaccggccc
     2461 cgcggcgccg gcgagcccga cctggtcgcc gagaccgtgg cggccgtcag ggaggtggtc
     2521 gccgactgcg ccgccgccgg gctgccgagc gtggtggagg cgctctccta cccgctgccc
     2581 ggcgaggcgc ccctcgaccc cggcgcgaag gccgccgtcg tgctcgagtc ggcccgactg
     2641 ctggcggcga cgggcccggc cctcctcaag ctcgagtacc cgggcggccc ggccgcctgc
     2701 cggcgggtga ccgacacggt cgggatgccc tgggcggtgc tctcggccgg ggtcggcttc
     2761 gacgagttcc tcggcaacct gcgggtcgcc atggacggcg gcgcctccgg cttcatcgcg
     2821 ggccgcgcga tctggaagga tgcgatgggg ctggacggcc aggagcgcac cgccttcctc
     2881 ggcgacgtcg ccaggcggcg gctcgacacg tgcgtccggg ccatggaggg ccgcggcagg
     2941 tccttcgccg aggccggccg ggcgatgggc agaggaggtg ggtgaggtga gcgagccgat
     3001 cctcgaggcg agagggatcg ccaagagcta cgggcacgtc ca
//
LOCUS       DASPYY010000203         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2284788, whole genome shotgun sequence.
ACCESSION   DASPYY010000203 DASPYY010000000
VERSION     DASPYY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284788"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..539)
                     /locus_tag="VEP73_03100"
     CDS             complement(207..539)
                     /locus_tag="VEP73_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYT25456.1"
                     /translation="MQSRLTDKDQDGMAKSWSWRLQAACRSEDTSLFFPADGERPGAK
                     ARREAKAKLICAACPVAAECAAYALGTHERWGVWGGLSQEEREAVWQRQEPRLALVDT
                     FTVQAVGL"
     gene            801..1001
                     /locus_tag="VEP73_03105"
     CDS             801..1001
                     /locus_tag="VEP73_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25457.1"
                     /translation="MARKRGSSDVDLVPEHSHEFEHEHRTGRGRRLVVLLALVGGIAY
                     YARRRQQRAALDEGVWHEAPPS"
     gene            1079..1147
                     /locus_tag="VEP73_03110"
                     /pseudo
     CDS             1079..1147
                     /locus_tag="VEP73_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            1252..>2574
                     /locus_tag="VEP73_03115"
     CDS             1252..>2574
                     /locus_tag="VEP73_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HYT25458.1"
                     /translation="MLDPPLPDQFVERMRRLLGAEADAFLASYGRPAERSARANTGKV
                     SARDLAALLGVDLEPVPWCEDASFLPEGVRVGATAAHAAGLFYIQDPSALAVVEALDV
                     RPGHRVLDLAAAPGGKTTHIASKLGTSGVVVANEVDSGRLQALADNLDRWGSWRVAIA
                     GETVERLARRLPGAFDRVLLDAPCSGEGLFRRSGAARVQWQPGHVRGSAERQRELLGQ
                     AARLVRPGGVLVYSTCTFAPEENERQVAAFLGAHGGWELLEIPWRPGFSPGRPDWSPG
                     GPAELARTVRLWPHLLRGEGHFVAKLGRRDGGAAPGPDGRDRPGRRDPSRAGGRRPRQ
                     PGGVLAAWEAFSADALTRRPAPSSTPTGARAGARVVGDRAYLVPDELIVPGDLRWLRP
                     GLLLGRSRPGRFEPAHALAMASTPSTVRRRRRLATGEASAWLRGEPLQA"
BASE COUNT          288 a          973 c          991 g          322 t
ORIGIN      
        1 cgtcgccccc ggcgtccgcg cgctggcgag cgccgcgctc gccgcggcgg gtagccgcgc
       61 cgcctaggcg ggagcttccg gccttcgagc agcgggtccg gggatctcgc ccgagccgcg
      121 agggatcagc cgagtcggca ccacgacgtg ccggggcgcc atgcgctcgc caacgagctg
      181 cccctgggac ggccccgccg cggtcctcac aggccgacgg cctggacggt gaaggtgtcg
      241 acgagggcga gcctgggctc ctgccgctgc cataccgctt cccgctcctc ctgcgacagc
      301 ccgccccaga cgccccagcg ctcgtgggtg ccgagggcgt acgcggcgca ctcggccgcg
      361 accgggcagg ccgcgcagat cagcttggcc ttcgcctcgc gccgcgcctt cgcgcccggc
      421 cgctcgccgt cggcgggaaa gaacagcgag gtgtcctccg agcggcaggc cgcctggagc
      481 cgccaggacc acgacttcgc catcccatcc tggtccttgt cggtcagccg gctttgcatc
      541 gtgtgctcct cgtgcaggtg gtctcgtcgt acacggtggt ctcgtcggtc ggatgctcga
      601 aggtcgcgac gtcggccgtc gctcgtgggt gccggagaag gctgagcggg cagcgcctct
      661 ggcatgggcg ggcggctcag ggtggcgcga gaggggttgc accgcgaccg tgcccggaaa
      721 cctgcccgca gtctcaaacc tgccggggac gaactagact cccacccgcc gacacggcca
      781 tcccctgagg aggaccttcc atggcgcgca agcgcggcag cagtgacgtc gacctcgtgc
      841 cggagcacag ccacgagttc gagcacgagc acaggacggg caggggcagg cggctcgtgg
      901 tgctgctcgc cctggtgggt ggcatcgcct actacgcccg tcgcaggcag cagcgggcag
      961 cgctcgacga gggcgtctgg cacgaggcgc cgccgtcgta gccgcgccgc cgccccgacc
     1021 cgtaggctgt acgccggcgg aggcgtggcg tggacggagg gagaaggagg cggcaccggt
     1081 gcgggtcgtg atcgccgagg actccgtgct gccgcgcgag gggctcgcgc gcctgctcac
     1141 cgaccgccgc cgggtcctcg ccgtcctcac ctacctgcga gcctgaccgt gcccgggcgg
     1201 cccagcgctc cgccgccccg gcggcccagc gctccgccgg gacccgccgc gatgctggac
     1261 ccgccgctgc ccgaccagtt cgtggagcgg atgcggcgcc tgctcggggc cgaggccgac
     1321 gcgttcctgg cgagctacgg gcggcccgcc gagcgctccg cgcgggccaa caccggcaag
     1381 gtgagcgctc gcgacctggc ggccctgctc ggcgtggacc tcgagccggt accgtggtgc
     1441 gaggacgcgt ccttcctgcc cgaaggggtc cgcgtcggcg cgacggccgc gcacgccgcc
     1501 ggactgttct acatccagga tccgtccgcg ctggccgtgg tggaggcgct cgacgtccgg
     1561 ccgggacacc gggtgctcga cctggccgcc gcgcccggcg gcaagaccac ccacatcgcg
     1621 agcaagctgg ggacgtcggg ggtggtggtg gccaacgaag tcgactccgg gcgcctgcag
     1681 gcgttggcgg acaacctcga ccgctggggg tcgtggcggg tcgcgatcgc cggcgagacc
     1741 gtcgagcggc tcgcgcggcg cctcccgggc gcgttcgacc gggtcctgct cgacgctccg
     1801 tgctccggag agggtctctt ccgccgcagc ggggcggccc gcgtccagtg gcaacctggc
     1861 cacgtgcggg gcagcgccga gcgccagcgg gagctgctcg ggcaggccgc ccgcctggtc
     1921 cgccccggcg gcgtgctcgt ctacagcacc tgcacgttcg cccccgagga gaacgagcgg
     1981 caggtggccg cgttcctcgg ggcccacggc ggctgggagc tgctggagat cccctggcgg
     2041 ccgggcttct ctcctggccg tcctgactgg tcccctggcg ggccggccga gctggccagg
     2101 acggtccgcc tgtggccgca cctgctgcgc ggcgaggggc acttcgtcgc caagctgggc
     2161 cgccgtgacg gcggtgcggc gcccggcccg gacgggcgcg accgcccagg gcggcgggac
     2221 ccgtcgcggg cgggaggacg gcggccacgc cagccgggcg gcgtcctggc ggcctgggag
     2281 gcgttctcgg cggacgcgct cacccgccgc ccggcgccct cgagcacgcc gacgggcgcg
     2341 agggccggcg cgagggtcgt cggcgatcgc gcgtacctgg tccccgacga gctgatcgtc
     2401 cccggcgacc tgcgctggtt gcggcccggc ctgctgctcg gccggagccg tcccgggcgg
     2461 ttcgagccgg cgcacgcgct cgccatggcc tcgaccccgt cgacggtccg gcggcgcagg
     2521 cggctggcca ccggcgaggc cagtgcctgg cttcgcggcg agcccctcca ggcc
//
LOCUS       DASPYY010000204         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2145036, whole genome shotgun sequence.
ACCESSION   DASPYY010000204 DASPYY010000000
VERSION     DASPYY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145036"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VEP73_03120"
     CDS             <1..891
                     /locus_tag="VEP73_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYT25459.1"
                     /translation="LRALPGIGEVTERVVREALAGEVPVYLRRLEAIEGRPVAEGAAA
                     LRAALRGDCHTHSNWSDGGEPVEVMAATARDLGHEYLVLTDHSPRLTVARGLDADRLR
                     RQLDEVAELNDRLAAEAAAGARAFRVLTGIEVDILDDGALDQSEELLARLDLVVASVH
                     SKLSMERAAMTRRMVAAVGNPHVDVLGHCTGRIVTGRGRPESEFDPGTVFAACARSGT
                     AVEVNSRPERSDPPKRLLRLAVEAGCRFAIDTDAHAPGQLDWQLGGCERAFACGVAPG
                     AVVNAMSADELLAWTRGKGG"
     gene            958..2262
                     /locus_tag="VEP73_03125"
     CDS             958..2262
                     /locus_tag="VEP73_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25460.1"
                     /translation="MELPDRSGTLLEGRALRLARAGWVAVVGLVLVLFVAGVPGSHAR
                     ARVLRPETLAGLDRLGIPAGAPAAYLTVLDVVTMLGFTAIAALIFVRRSDDWIAMLMA
                     LTLVLTAMLYTAPVFEASVPPVLIAMLCALGELCQLAVVYLFPSGRFVPRWVWPPLAL
                     LVLWRPAMWALVYMPQFLASERTGENYAYVPQDARDIALVLVLFATGFATQAYRYRHV
                     SSPLQRQQTKWLLFGMLGTVAIAGAYIFVSIATVGPSPELGSEALLVRLGGRTVRQLA
                     LFLVPVTVTYSILRHRLWDIDKLINRALVYSALTAVLGAGYAAMVVGLQNVLSRVAGG
                     SSLAVAASTLTAAALFRPARRRVQDVVDRRFDRGRVDAVRTIEAFSARLRDEVDLNTI
                     SADLLGVVDATMQPTHVSLWLRPVPDQADGRRTAQPGRQAGG"
BASE COUNT          282 a          973 c          940 g          371 t
ORIGIN      
        1 ctgcgggcac tgcctggcat cggcgaggtc accgagcggg tcgtccgcga ggcgctggcc
       61 ggcgaggtcc ccgtctacct gcgccggctc gaggcgatcg aggggcgccc ggtggccgag
      121 ggcgccgccg cgctgcgcgc agccctgcgc ggcgactgcc acacccactc gaactggtcg
      181 gacggtgggg aaccggtcga ggtcatggcg gcgaccgcgc gggacctggg acacgagtac
      241 ctcgtcctca ccgaccactc gccccggctc accgtggccc gcggcctcga cgcggaccgg
      301 ctgcgccgcc agctcgacga ggtcgccgag ctgaacgacc ggctggccgc ggaggccgcc
      361 gccggggcgc gggcgttccg ggtcctgacc gggatcgagg tcgacatcct cgacgacggc
      421 gccctcgacc agtccgagga gctgctcgcc cgcctcgacc tggtggtcgc gagcgtccac
      481 tccaagctca gcatggagcg ggcggcgatg accaggcgga tggtcgccgc cgtcggcaac
      541 ccgcacgtcg acgtgctcgg ccactgcacg ggcaggatcg tgaccggcag gggccggccg
      601 gagtccgagt tcgaccccgg gacggtgttc gccgcatgcg cccggtcggg cacggcggtc
      661 gaggtgaact cccgcccgga gcgctccgac ccgcccaagc ggctgctgcg cctggccgtc
      721 gaggcgggct gccggttcgc gatcgacacc gacgcccacg ccccgggcca gctcgactgg
      781 cagctcggcg gctgcgagcg ggcgttcgcg tgcggcgtcg cgcccggcgc ggtcgtcaac
      841 gccatgtccg ccgacgaact gctcgcctgg acgcgcggga agggtggctg acgccgcacg
      901 ccgtctggta tgaacggacc cgtccgggaa tgtgcgccgg ctcgtggagc gccagggatg
      961 gagctgcctg accgatcggg gaccctgctc gagggccgcg cgctgcggct cgcccgggcg
     1021 ggctgggtcg ccgtggtcgg cctggtcctc gtgctgttcg tcgccggcgt ccctggcagc
     1081 cacgccaggg cgcgcgtcct ccggccggag acccttgcgg ggctcgatcg gctcggcatc
     1141 ccggccggcg cgccggccgc gtacctgacc gtcctcgacg tggtcacgat gctcggcttc
     1201 acggccatcg ccgcgctcat cttcgtgcgc cggtcggacg actggatcgc gatgctgatg
     1261 gcgctcacgc tcgtgctgac cgccatgctg tacaccgcgc cggtgttcga ggcgtccgtg
     1321 ccgcccgtcc tcatcgcgat gctctgcgcg ctcggcgagc tctgccagct ggccgtcgtc
     1381 tacctgttcc cgagcggccg cttcgtcccg cgctgggtgt ggccgccgct ggccttgctg
     1441 gtgctgtggc gcccggcgat gtgggccctg gtctacatgc cgcagttcct cgcctccgag
     1501 cgcaccggcg agaactacgc ctacgtcccg caggacgcgc gggacatcgc gctcgtgctc
     1561 gtcctgttcg ccaccggctt cgcgacccag gcgtaccgct accggcacgt ctcctcgccg
     1621 ctccagcggc agcagaccaa gtggctgctg ttcggcatgc tgggcacggt cgcgatcgcg
     1681 ggcgcgtaca tcttcgtgag catcgccacc gtcggcccgt cgcccgagct cggctccgag
     1741 gcgctgctcg tacggctcgg cggccggacc gtcagacagc tcgccctgtt cctcgtcccg
     1801 gtcaccgtga cctactcgat cctccgccac cggctgtggg acatcgacaa gctcatcaac
     1861 cgggcgctcg tctacagcgc cctgacggcg gtcctcggag ccggctacgc ggccatggtg
     1921 gtcgggctgc agaacgtcct cagccgggtc gccggcgggt ccagcctcgc cgtggcggcg
     1981 tccaccctca ccgcggcggc gctgttccgg ccggcccgcc gccgcgtgca ggacgtggtc
     2041 gaccggcggt tcgaccgggg gcgcgtcgac gcggtcagga ccatcgaggc gttcagcgcc
     2101 cggctgcggg acgaggtcga tctcaacacc atctcggccg acctgctcgg ggtcgtggac
     2161 gcgaccatgc agcccacaca tgtgtcgctg tggctgcggc ccgtgccgga tcaggccgac
     2221 gggcggcgga ccgcccagcc gggccgccag gccggcggat gaccccggcg cacgcgtcgg
     2281 cggcgctccg ccgcgccgag taccgcttct cccgtgcgct gaatatgctg agggcatgtg
     2341 cgacgatccg cgcggtgggg ccgcgccgcc acgcgtgccg cccgcggcga tgaagccgcg
     2401 gctccggggg cggctgcacc aggtcgcgtt cttcgcgtcg atcccggccg gcgccgcgct
     2461 ggtgctcgtg gcccggaccg cgccggcccg ggtcgcggcc gcggtctacg cgctcagcct
     2521 ggccggcctg tacggcacga gcgccgcgtt ccacctcggg cggtgg
//
LOCUS       DASPYY010000205         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2145149, whole genome shotgun sequence.
ACCESSION   DASPYY010000205 DASPYY010000000
VERSION     DASPYY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145149"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="rfaE2"
                     /locus_tag="VEP73_03130"
     CDS             <1..590
                     /gene="rfaE2"
                     /locus_tag="VEP73_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HYT25461.1"
                     /translation="AAGGAAAVGLGHGRGGGAGSVEATAPGSSGDGGPPRAEDVVAVV
                     RARGGTVVATGGCFDLLHAGHVATLRAARALGDCLVVCLNSDASVRRLKGPGRPLVAE
                     ADRAAVLAALGCVDAVAVFDERTPEAVLDRLRPDVFAKGGDYALSDLPEAALLASWGG
                     QAVVLPYLPGRSTTELMTELMTELMKKEVVRRDNR"
     gene            577..1284
                     /locus_tag="VEP73_03135"
     CDS             577..1284
                     /locus_tag="VEP73_03135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT25462.1"
                     /translation="MTTGEKAAGGLGTVLVTGGSSGLGAAVARAVADAGGTPVVVDRV
                     PPPDGVEHELADLADGRAAEAAVRAVAEQHAGLGAVVTAAGTDRCGTLAEVPADAWER
                     VVLVNLLGTAAVVRAAWPYLERSHGKVVTVASTLGLRAVSDATAYCASKFGVVGFTRA
                     LAAEAAGRVGVTLLVPGGMHTAFFDDRSEQYRPPADAKLNRPEDVAAAVVFALCQPPG
                     CEVRELVVAPSTETSWP"
     gene            1281..2255
                     /locus_tag="VEP73_03140"
     CDS             1281..2255
                     /locus_tag="VEP73_03140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYT25463.1"
                     /translation="MRSPTLVALRALGLGDLLTAVPALRALADAFPGHRRLLATRPAL
                     APLARCTGTVDEVVPADGLAGPPDARLYGADVAVNLHGRGPESHRLLLGTGPRRLLAF
                     AHPAVPATAPGPRWRDDEHEVARWCRLLAESGVPADPGRLDLELPAGPVPGDVRGATL
                     LHPGAGSPARRWPPARFAAVAAAERARGRGVAVTAGPGEAALARDVARRAGLPGRAVH
                     AVGDDVLALGRLVAAAGRVVCGDTGVAHLATALGTPSVVLCGPVSPARWGPPADRPAV
                     HRALWAGRGGDPHGRRPDPGLLALSVGDVMAALEALDARPRPSGSRPA"
BASE COUNT          221 a          932 c          964 g          261 t
ORIGIN      
        1 tcgccgccgg cggcgccgcc gccgtcggcc tgggccacgg ccgcggcggg ggggccggct
       61 ccgtggaggc gaccgccccg ggctcgagcg gggacggggg cccgccacgg gccgaggacg
      121 tggtcgccgt ggtgcgcgcc aggggcggga ccgtggtcgc caccggcggg tgcttcgacc
      181 tgctgcacgc cgggcacgtg gcgaccttgc gggccgcccg cgcgctcggc gactgcctgg
      241 tcgtctgcct caactccgac gcctcggtgc gccgcctgaa gggccccggg cggccgctgg
      301 tcgccgaggc ggaccgggcg gcggtgctgg ccgccctcgg gtgcgtcgac gccgtggccg
      361 tgttcgacga gcggaccccg gaggcggtgc tcgaccggct ccggccggac gtgttcgcca
      421 agggtgggga ctacgcgctg tccgacctgc cagaggcggc cctgctcgcc tcgtggggcg
      481 gccaggcggt ggtcctcccc tacctacccg ggcgctccac caccgagctg atgaccgagc
      541 tgatgaccga gctgatgaag aaggaggtcg tccgtcgtga caaccggtga gaaggcagca
      601 ggcgggctcg gcaccgtgct cgtgaccggc gggtcgtccg gcctgggtgc ggccgtcgcg
      661 cgggcggtcg ccgacgcggg cggcacgccg gtcgtggtcg accgggtccc gcccccggac
      721 ggcgtggagc acgagctggc cgacctggcc gacggcaggg cggccgaggc cgcggtgcgg
      781 gcggtggccg agcagcacgc gggcctgggc gcggtggtga ccgccgcggg gaccgaccgc
      841 tgcggcacgc tcgcggaggt gcccgccgac gcatgggagc gggtcgtgct cgtcaacctg
      901 ctcggcaccg ccgcggtggt gcgcgccgcg tggccgtacc tcgagcgctc gcacggcaag
      961 gtggtcacgg tcgcgtccac cctcgggctg cgcgcggtga gcgacgccac cgcctactgc
     1021 gcgagcaagt tcggcgtggt cggcttcacc cgggcgctcg ccgccgaggc cgccggacgt
     1081 gtgggggtca ccctgctcgt ccccggcggc atgcacaccg cgttcttcga cgaccgcagc
     1141 gagcagtaca ggccgcccgc ggacgcgaag ctcaaccggc cagaggacgt ggccgcggcc
     1201 gtcgtgttcg cgctctgcca gccgcccggc tgcgaggtcc gcgagctggt cgtcgcgccg
     1261 tccacggaga cgtcctggcc gtgaggtcgc cgaccctggt cgcgctgcgc gcgctcgggc
     1321 tcggcgacct gctgaccgcc gtgcccgccc tgcgggccct ggccgacgcg ttccccggcc
     1381 accgccgcct gctcgccacc cgccccgcgc tcgcgccgct cgcgcgctgc acgggcacgg
     1441 tcgacgaggt ggtgcccgcc gacgggctcg ccggcccgcc cgacgcgcgc ctctacggcg
     1501 ccgacgtggc cgtcaacctg cacggaaggg ggccggagag ccaccgcctg ctgctcggga
     1561 ccggacctcg gcggctgctc gcgttcgccc acccggccgt ccccgcgacc gcaccgggtc
     1621 cgcggtggcg ggacgacgag cacgaggtcg cccgctggtg ccgcctgctc gccgagagcg
     1681 gcgtccccgc cgaccctggc cgcctcgacc tcgagctgcc cgccgggccg gtgccagggg
     1741 acgtccgcgg cgccaccctg ctccaccccg gggccgggag cccggcgcgg cgctggccgc
     1801 cggcgcgctt cgccgccgtc gcggcggcgg agcgcgccag ggggcgaggc gtcgcggtga
     1861 ccgccgggcc gggcgaggcg gcgctcgcgc gcgacgtcgc ccgccgggcc ggcctgcccg
     1921 ggcgggccgt gcacgcggtc ggggacgacg tgctcgcact cggccgcctc gtcgccgccg
     1981 ccgggcgggt ggtctgcggc gacaccggcg tggcgcacct ggccaccgcg ctcggcacgc
     2041 cgtcggtggt gctgtgcggc ccggtctccc cggcgcggtg ggggccgccg gccgaccggc
     2101 ccgccgtgca ccgggcgctc tgggcgggcc gcggcgggga cccgcacggc cgccggccag
     2161 accccggcct gctcgccctc tcggtcggcg acgtcatggc cgcgctcgag gcgctcgacg
     2221 cgcggccgcg accctcgggg tctcgcccgg cgtagcgggc gggcaggcgc cgcggccgtc
     2281 cgcgacagca cgcgggctcc ctcaagaact ccgccgggcc gccgaacgag accgggagca
     2341 gggccgtgac ggagcgagcc ggggagcggg cgtggacg
//
LOCUS       DASPYY010000206         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_178933, whole genome shotgun sequence.
ACCESSION   DASPYY010000206 DASPYY010000000
VERSION     DASPYY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178933"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..>1697)
                     /locus_tag="VEP73_03145"
     CDS             complement(76..>1697)
                     /locus_tag="VEP73_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25464.1"
                     /translation="RLTPFQLSFVFPAVMPAVVGLGAGAFAVMALQEPRWTFPVYALI
                     VGGSLNAAVMAISHSDTLIVSAVAMAAAATSLAAAGGERTIGASVFLLAGATLVHWNF
                     VAVFLGILAVLAVALVPESLAARRRGAGMRTTPAARIGTVVLGSAVLGGGALLLAPGG
                     LQQPPPARDLFLRRMTAIVPVYELPITGPIAAAGALSLWRPKDPARRRALWFALIWCG
                     SAVAATALLYVGAPVAAHRILGFAYALPLLATVALVWAARAGWRAGRTVPRAAGIAVL
                     AGGLALSAFATERQWFRSRASLPAATFAQAATAGAYLERYAPGRPAVVVIDGAPGLVP
                     LAERVFRASVPADRIATTLPYLGDPDRLLAGQPTVRGEPAFDAASRLNLDRLEPLLSK
                     DPVVVMVSSLDRRYRALTQANPAWVVAPGVAVVSGPRPAAPLSAAPAPRAVPGRSLVL
                     RAGAVLLLLSVVGVGWAWSLIPAGWFERLALAPAFGVAALSLAGVAADRLGMVFPGPS
                     GIGVAAGAAAAGWAARILVRAVPPRPAPTTP"
BASE COUNT          254 a          659 c          644 g          140 t
ORIGIN      
        1 cgagcgcgac cgcggccggc aggccgagct ccagggacct ccggcccgcc cgcgaacggt
       61 ggtccgccgg cgagctcatg gagtggtcgg tgcgggtcgg ggcgggactg cccggaccag
      121 gatccgcgcc gcccatccgg cggccgcggc cccggcggcc acgccgatcc ccgatggtcc
      181 cgggaagacc atgccgaggc ggtccgccgc gacccccgcc agcgacagcg cggcgacgcc
      241 gaacgcgggg gccagggcca gccgttcgaa ccagcctgcc gggatcaggc tccacgccca
      301 tccgaccccg acgaccgaga ggagcagcag cacggcgccg gctcgcagca cgagcgaccg
      361 cccgggcacg gctcgcgggg ccggcgccgc gctcagcggg gccgccggcc gagggccgct
      421 gacgacggcg accccgggcg ccaccaccca ggcggggttc gcctgggtga gcgctcggta
      481 gcgccggtcg agcgacgaga ccatcaccac caccgggtcc ttcgacagga gcggttcgag
      541 gcgatcgagg ttcaaccgcg acgctgcgtc gaaggccggc tcgccgcgga cggtgggctg
      601 ccccgccagg agccggtccg ggtcgcccag gtaggggagc gtcgtggcga tccggtcggc
      661 cggcacggag gccctgaaca cgcgttcggc gagcgggacg agtcccggcg ctccgtcgat
      721 cacgaccacc gccggccggc ccggcgcgta ccgctcgagg tacgccccgg cggtggccgc
      781 ctgggcgaac gtcgccgcgg ggagggaggc gcgcgagcgg aaccactgcc gctcggtggc
      841 gaacgccgag agcgcgagcc ctccggccag gaccgcgatc ccggccgcac gcgggaccgt
      901 cctcccggcg cgccagccgg cgcgggccgc ccacaccagc gcgacggtcg ccagcagcgg
      961 cagcgcgtag gcgaacccca ggatccggtg ggcggcgacg ggagctccca cgtacaggag
     1021 cgccgtcgcg gccaccgccg acccgcacca gatcagggcg aaccacaggg cccgtcgccg
     1081 ggccgggtcc ttcggcctcc agagcgagag ggcgccggcc gcggcgatgg ggcccgtgat
     1141 gggcagctcg tagacgggca cgatcgccgt catccgccgc aggaagaggt cccgagcggg
     1201 cgggggctgc tgcagacccc cgggggcgag cagcagcgcg ccgcctccca ggaccgccga
     1261 tcccagcacc accgtcccga tccgcgccgc cggcgtcgtc cgcatgcccg caccacgccg
     1321 tcgggcggcc agcgactccg ggacgagcgc cacggccagc accgcgagga tcccaaggaa
     1381 gacggccacg aagttccagt gcacgagcgt cgcgccggcc agcaggaaca cgctcgcccc
     1441 gatcgtccgt tcgccgccgg ccgcggcgag cgacgtggcg gcggccgcca tcgccaccgc
     1501 cgagacgatc aacgtatcgc tgtggctgat ggccatgacc gccgcgttca gcgagccgcc
     1561 gacgatcagc gcgtagacgg ggaaggtcca ccgcggctcc tgcagggcca tgacggcgaa
     1621 cgcgcccgcg ccgagcccga cgaccgccgg catcacggcc gggaagacga acgacagctg
     1681 gaacggcgtc aggcgga
//
LOCUS       DASPYY010000207         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_318573, whole genome shotgun sequence.
ACCESSION   DASPYY010000207 DASPYY010000000
VERSION     DASPYY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318573"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEP73_03150"
     CDS             <1..207
                     /locus_tag="VEP73_03150"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922451.1"
                     /note="catalyzes the oxidation of 3-isopropylmalate to
                     3-carboxy-4-methyl-2-oxopentanoate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HYT25465.1"
                     /translation="SAALLLEHLGEAGAAAAVEAAAVAALPGAGRYSTRELGDQIAVA
                     VADEVPAPPEAGDGVAPGSRPSGP"
     gene            264..1184
                     /locus_tag="VEP73_03155"
     CDS             264..1184
                     /locus_tag="VEP73_03155"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HYT25466.1"
                     /translation="MPIEATSKIWMDGEYVDWDAARVHVLTPSLHYGWGAFEGIRAYE
                     TDSGSSAVFRLTDHMKRLFRSAKIYHMEPPISLEGSIKVVKDLVAMNGVRTCYIRPIV
                     YLGYGEIGLNPLVSRVSMAIACWPWGAYLGEEAFQTGVRVKVSSWRRLDPNIIPPAAK
                     ATGQYLNSSLAKAEAVKAGYDEGILLNPNGYVTDGSGENLFVVRGGALCTPPLAAGCL
                     AGITRDSIIRIAADEGIEVQQSDLVRTDLYLADEAFFTGTAAEVVPIREVDDRVLGDP
                     GPITRRVQEVFVAATRGRDERYSDWLEPVE"
     gene            1288..2952
                     /gene="cimA"
                     /locus_tag="VEP73_03160"
     CDS             1288..2952
                     /gene="cimA"
                     /locus_tag="VEP73_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HYT25467.1"
                     /translation="MTDPRPVPAPARDPGRVETYDTTLRDGSQQVGLTFTVADKLRVT
                     QLLDDLGVDVVEGGWPGSNPKDAEYFARVRDLELRHAVLSAFGATRRPGRGVEDDANL
                     RALLDSGAPVVALVGKSWTLHVTEALRTTFDENLAMVADSVALMVAEGRRVVFDAEHF
                     FDGWAADPAYALAVVRAAADAGADTVALCDTNGGTLPTTVARVVGEVVRALPGVRVGG
                     HFHDDAACAVANSLVAVAGGASHVQGAANGYGERCGNADLFAVVAGLELKEGRKVLPP
                     GRLSALTATAHAIAELANFVPDARQAYAGSSAFATKAGLHASAIARRPDAYSHVDPAS
                     VGNRMRVLVSELAGRSNVLAKAGELGLDLEADAGLGARVLDRVKELEHQGYTFEIADA
                     SFELLVRREAGWHAPPFTLEGYRVLVERRPDGTDVSEATVRVLLPDPEAGPEGEDPSR
                     GRRTVAVGEGVGPVHALDQALRRALADDRPELESVRLVDFKVRILDGRAGTKAVTRVL
                     ITSADPAGEWTTVGVSDDIVTASWEALADGIEYGLLRARAPAAVRG"
     gene            3037..>3555
                     /locus_tag="VEP73_03165"
     CDS             3037..>3555
                     /locus_tag="VEP73_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25468.1"
                     /translation="MARARWRRPAVALCAVALALAGCQGGRRATPAPPSTAAPAPGAA
                     DTTGPAASTRATGYTAPLDLSTRSPSGLSMKLTAIAVAEDSISLEVVVTNGSQLDRAL
                     NNADDLVLVDDKGTSYDVSPPPDNQSVTVRHGTTMRGRLVFAGQLDPAATTLTLVANS
                     TSAGDPRYSPHPK"
BASE COUNT          476 a         1368 c         1265 g          446 t
ORIGIN      
        1 tccgccgccc tgctgctcga gcacctcggc gaggcggggg cggccgccgc ggtcgaggcc
       61 gcagccgtgg cagccctgcc aggtgctggg cgatactcca ccagagagct tggggaccag
      121 atcgccgtcg cggtcgcgga cgaggtaccc gccccgcccg aagccggcga cggagtggct
      181 cccgggagcc gaccgtccgg accctagaca acgtcgaacc gcaacggcgc ggcgccgttc
      241 ggctctttga gggaggttca ccgatgccga tcgaggcgac cagcaagatc tggatggacg
      301 gcgagtacgt ggactgggac gccgcccgcg tccacgtcct caccccgtcg ctgcactacg
      361 ggtggggggc cttcgagggc atccgcgcgt acgagacgga ctcggggtcc tcggccgtgt
      421 tccgcctcac cgaccacatg aagcggctgt tccgcagcgc caagatctac cacatggagc
      481 cgcccatctc gctcgagggc tccatcaagg tggtcaagga cctcgtggcg atgaacgggg
      541 tccgcacctg ctacatccgc cccatcgtct acctcgggta cggcgagatc gggctcaacc
      601 cgctcgtctc gcgcgtctcc atggccatcg cctgctggcc ctggggcgcc tacctgggcg
      661 aggaggcgtt ccagaccggc gtccgcgtca aggtcagctc gtggcggcgg ctcgacccga
      721 acatcatccc gcccgcggcc aaggccaccg gccagtacct gaactcctcc ctggccaagg
      781 ccgaggcggt caaggccggg tacgacgagg gcatcctgct gaacccgaac ggctacgtga
      841 ccgacggctc gggcgagaac ctgttcgtcg tgcgcggcgg cgcgctgtgc accccgccgc
      901 tcgcggccgg ctgcctggcc ggcatcacca gggacagcat catacgcatc gcggccgacg
      961 aggggatcga ggtgcagcag tccgacctcg tgcgcactga cctctacctg gccgacgagg
     1021 ccttcttcac cgggacggcc gccgaggtcg tgcccatccg ggaggtggac gaccgggtcc
     1081 tcggcgaccc ggggccgatt acccgccggg tgcaggaggt cttcgtcgcc gcgacccgcg
     1141 gccgcgacga gcgctacagc gactggctcg agccggtcga gtagcgcggc gccccgcctc
     1201 ccgcaccctc ccgccagtgc cggcgccgcc gccggtttcc ctgcagcctg cgctccggag
     1261 ggctctcccg tgaccttcga cctgcccgtg accgatcccc gtcccgtacc cgcgcccgct
     1321 cgtgaccccg ggcgggtgga gacctacgac acgaccctgc gcgacggctc gcagcaggtc
     1381 gggctcacct tcaccgtggc cgacaagctg cgggtcaccc agctcctcga cgacctcggc
     1441 gtcgacgtcg tcgagggggg gtggccgggc tcgaacccga aggacgccga gtacttcgcc
     1501 cgcgtgcgcg acctcgagct gcgccacgcc gtgctgagcg cgttcggcgc cacccgccgg
     1561 ccggggcggg gtgtcgagga cgacgccaac ctgcgcgccc tgctcgactc gggcgcgccc
     1621 gtggtcgcgc tcgtcggcaa gtcgtggacc ctccacgtca ccgaggcgct gcgcaccacc
     1681 ttcgacgaga acctggcgat ggtcgccgac tcggtcgcgc tgatggtcgc cgagggccgc
     1741 cgggtggtgt tcgacgccga gcacttcttc gacgggtggg cggccgaccc cgcctacgcg
     1801 ctggcggtcg tccgggccgc ggccgacgcc ggcgcggaca ccgtggcgct ctgcgacacc
     1861 aacggcggca ccctgccgac caccgtcgcc cgggtcgtcg gggaggtcgt ccgcgccctg
     1921 cccggcgtcc gcgtcggcgg gcacttccac gacgacgcgg cctgcgccgt ggcgaactcg
     1981 ctcgtcgcgg tcgccggcgg cgccagccac gtccagggcg cggccaacgg ctacggggag
     2041 cggtgcggca acgccgacct gttcgcggtc gtggccgggc tcgagctgaa ggaagggcgc
     2101 aaggtgctcc cgcccgggcg gctgtcggcg ctcacggcga ccgcgcatgc catcgccgag
     2161 ctggccaact tcgtgcccga cgcgcgccag gcgtacgccg gctcgtccgc gttcgcgacc
     2221 aaggccggcc tgcacgcctc ggcgatcgcc cgaaggccgg acgcctactc ccacgtcgac
     2281 ccggcctcgg tcggcaaccg catgcgggtg ctcgtctcgg agctcgccgg gcgcagcaac
     2341 gtgctcgcca aggcgggcga gctgggcctc gacctcgagg ccgacgccgg cctcggcgcc
     2401 cgggtgctcg accgggtgaa ggagctcgag caccagggct acacgttcga gatcgccgac
     2461 gcctcgttcg agctgctcgt ccgccgcgag gcggggtggc acgcgccccc gttcacgctc
     2521 gagggctacc gggtcctggt ggagcggcgt cccgacggca ccgacgtgag cgaggcgacc
     2581 gtccgggtcc tgctcccgga cccggaggca ggccccgagg gcgaggaccc ctcccgaggc
     2641 cgccgcaccg tcgcggtcgg cgagggcgtg gggccggtgc acgccctcga ccaggccctc
     2701 cggcgggcgc tcgcggacga ccgccccgag ctcgagtccg tgcgcctggt cgacttcaag
     2761 gtgcgcatcc tcgacggccg ggccggcacg aaggcggtga cccgggtgct catcacctcg
     2821 gccgacccgg cgggggagtg gacgacggtc ggggtctccg acgacatcgt gaccgcctcc
     2881 tgggaggcgc tcgccgacgg catcgagtac gggctgctcc gcgcccgcgc cccggcagcc
     2941 gtccggggat agcagcgacc gagcgccggg ttggcaaggc agggaacgac cgcgacaatc
     3001 gtcccccgac cggaaccgct cgaggaggtg aggtccgtgg cccgggcccg gtggcgccgg
     3061 cccgccgtgg cgctctgtgc tgtcgcgctc gccctcgccg gctgccaagg ggggaggcgc
     3121 gcgacccccg ctccgccgag caccgccgcg ccggccccgg gcgcggcgga cacgaccggc
     3181 ccggcggcgt ccacccgcgc caccgggtac acggcaccgc tcgacctctc gaccaggagc
     3241 ccgagcgggc tgtccatgaa gctgaccgcg atcgcggtcg ccgaggactc gatcagcctc
     3301 gaggtggtcg tcaccaacgg cagccagctc gaccgcgcgc tcaacaacgc cgacgacctc
     3361 gtgctcgtcg acgacaaggg cacgagctac gacgtctccc cgccgcccga caaccagtcc
     3421 gtgaccgtcc gccacggcac caccatgcgc gggcggctgg tgttcgccgg ccagctcgac
     3481 ccggccgcca cgacgctcac cctggtcgcg aactcgacgt cggcggggga cccgaggtac
     3541 tcgccccacc cgaag
//
LOCUS       DASPYY010000208         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2707247, whole genome shotgun sequence.
ACCESSION   DASPYY010000208 DASPYY010000000
VERSION     DASPYY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2707247"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VEP73_03170"
     CDS             complement(<1..1091)
                     /locus_tag="VEP73_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYT25469.1"
                     /translation="MRVGSWGGRPVPVVLLLAAALALGACRSGPDRRFAADTVRTADV
                     TERINAPGSLEAAAQADLMAPAAGKVDRLAVTDGARVHQGQVVAELSSSQVDDQISQA
                     RSALAAASSLGSAVPTLPTGSAPSLLGDVQSQVASSSAAVLTALRGLVPLLPEPQRGQ
                     FQAQLDQAQERIATAQLRADQAARQAAAAANEQVDAVRRSVDAATAAQRAQARVALAV
                     AERQRDGLTLRAPLSGTVQLGRSGSRGGSPLAGLPSLPAGAEQALQGLAGSGGGGGQT
                     GPVLRPGAEVTAGQVVATVYDVAGLDVATSVDETDVALVRVGQAALVELDAFPGTRFA
                     ARVRRVAVAPSSSAQSTSGGVTYEVDLALG"
     gene            complement(1273..2184)
                     /locus_tag="VEP73_03175"
     CDS             complement(1273..2184)
                     /locus_tag="VEP73_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYT25470.1"
                     /translation="MLKATLLKAAGSPTLKRQMTANPVARRVAGRFIAGATIGDAERV
                     IRELNAKGMSVALDHLGENTGSQAQAREAAAAYLAALDRIQEHGLDANISVKLTALGL
                     DIRPELALEEASKVAARGKEVGAMVGVDMESHAYVERTLETVRRLSASYDDVGVCVQA
                     YLYRTRDDLDRLNDLGVPVRLVKGAYREPPEVAYPHKASVDGAYARLLDSLIQANPYP
                     MVATHDPALVRLAKTLVARYARDRDTFEFQMLYGVRRDLQEQVLAEGYRLRVYVPYGD
                     QWYPYFMRRLAERPANLYFFLSNLAKR"
     gene            2528..>2774
                     /locus_tag="VEP73_03180"
     CDS             2528..>2774
                     /locus_tag="VEP73_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25471.1"
                     /translation="MRRQQHRPERLASRTRRRVLAAAISLAVAVVMTAGTALTAGAAN
                     AAPSGAVSAATTTIRILRIGMTGADVLALQKRLVQLHY"
BASE COUNT          334 a         1027 c         1049 g          364 t
ORIGIN      
        1 ccgagggcga ggtcgacctc gtaggtgacc ccgcccgagg tggactgggc cgaggaggag
       61 ggcgccaccg cgaccctgcg cacccgggcc gcgaaccggg tgcccgggaa ggcgtcgagc
      121 tccaccaggg cggcctggcc gaccctgacc agggccacgt cggtctcgtc gacggacgtg
      181 gccacgtcca ggccggcgac gtcgtagacg gtggcgacga cctggccggc ggtcacctcg
      241 gcgcctgggc gcagcaccgg gccggtctgg ccgccaccac caccggagcc ggcgaggccc
      301 tggagcgcct gctcggcccc tgccggcagc gacgggaggc cggccagggg gctcccgccg
      361 cgcgacccgg accggccgag ctgcaccgtg cccgagaggg gcgcccgcag ggtgaggccg
      421 tcccgctggc gctcggcgac cgcgagggcg acccgggcct gggcgcgctg ggccgccgtg
      481 gcggcgtcga ccgagcggcg cacggcgtcc acctgctcgt tcgcggccgc tgcggcctgc
      541 ctggccgcct ggtcggcgcg cagctgcgcg gtcgcgatgc gctcctgggc ctggtcgagc
      601 tgggcctgga actggccgcg ctggggctcg gggagcagcg gcacgagccc ccgcagggcg
      661 gtcagcacgg cggcgctgga gctggcgacc tgggactgga cgtcgccgag gagggagggt
      721 gccgacccgg tgggcagggt cggcaccgcc gagccgagcg acgaggcggc cgcgagggcc
      781 gagcgggcct ggctgatctg gtcgtcgacc tggctcgacg agagctcggc gacgacctgc
      841 ccttggtgca cccgcgcgcc gtcggtcacg gccaggcggt cgaccttgcc ggcggcgggc
      901 gccatgaggt cggcctgggc cgcggcctcg agcgagccgg gggcgttgat gcgctcggtc
      961 acgtccgccg tccgcaccgt gtcggcggcg aagcgccggt caggtccgga cctgcatgcg
     1021 ccgagggcca gcgcggcggc gagcaggagc accacgggca ccgggcgccc gccccaggac
     1081 cccacccgca tccggcctct ctttccgttc ggcacgcctt gctctgtccc gttcggcacg
     1141 ccgaggcgtg cctccgcccc tctctttccc gttcggcacg ccgaggcgtg cctccgccct
     1201 ccacgagcat agaggcgccc tccgagttgc gcccccatcc ggcgcggggc acgggcgccg
     1261 gcggcgggag cgctatcgct tcgccaggtt cgagaggaag aagtagaggt tggcgggccg
     1321 ctcggccagc cggcgcatga agtaggggta ccactggtcc ccgtagggga cgtagacccg
     1381 caggcggtag ccctcggcga gcacctgctc ctggaggtcg cggcggaccc cgtagagcat
     1441 ctggaactcg aaggtgtccc ggtcccgggc gtatctcgcc acgagcgtct tggcgaggcg
     1501 gacgagggcc gggtcgtggg tggccaccat cgggtacggg ttcgcctgga tcaggctgtc
     1561 gaggaggcgg gcgtacgcgc cgtcgacgct cgccttgtgg gggtaggcga cctcgggcgg
     1621 ctcccggtag gcgcccttga ccagccggac gggcacaccc aggtcgttca gccggtcgag
     1681 gtcgtcgcgg gtgcggtaca ggtaggcctg cacgcacacg ccgacgtcgt cgtaggacgc
     1741 cgagagccgg cggaccgtct cgagggtgcg ctcgacgtag gcgtgggact ccatgtcgac
     1801 gccgaccatg gcgccgacct ccttgcccct ggcggcgacc ttggacgcct cctcgagcgc
     1861 gagctcgggc cggatgtcca gtcccagcgc ggtcagcttg accgagatgt tcgcgtccag
     1921 gccgtgctcc tggatgcggt cgagcgccgc caggtaggcg gcggccgcct cgcgagcctg
     1981 ggcctgcgac cccgtgttct cccccaggtg gtcgagggcg acggacatgc ccttcgcgtt
     2041 cagctcgcgg atgacccgct ccgcgtcgcc gatcgtcgca ccggcgatga agcggccggc
     2101 cacgcggcgg gcgaccgggt tcgcggtcat ctgccgcttg agggtcggtg acccggcggc
     2161 cttcagcagc gttgccttga gcacgcctcc acctcgcttt cgagcacgcc tcgctcggag
     2221 gccgtccagc ggtcggagcc agcgtcctgg ctggtcaggc gctggagggg tgctgccccg
     2281 aggccagtat ggcatggggc tcccgccccc cggggacctc tccggccccg cccccggccg
     2341 cggcggcgca cgggcatcgc ggcccgttgt gatgttgaga ccgttgtggc agcgcttcgc
     2401 gacctcgtac agtcgtaata gtcggacaac acgagcttcg caccggagtc tcccggttcc
     2461 gccgcatcat ctgacccgcg tcccgcccgc accgcctgac ccgcatcgcc cgaatggagt
     2521 gcagcccatg cgtcgccagc agcaccgccc cgaacggctc gcctcgcgca cccggcggcg
     2581 cgtgctcgcc gccgccatca gcctcgccgt ggccgtggtc atgacggccg gcacagcgct
     2641 gaccgcgggc gcggcgaacg cggcgccgtc cggcgcggtc agcgccgcga ccacgaccat
     2701 ccgcatcctg cgcatcggca tgaccggcgc cgacgtgctc gccctgcaga agcggctggt
     2761 gcagctccac tacg
//
LOCUS       DASPYY010000209         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_460419, whole genome shotgun sequence.
ACCESSION   DASPYY010000209 DASPYY010000000
VERSION     DASPYY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460419"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1037)
                     /locus_tag="VEP73_03185"
     CDS             complement(120..1037)
                     /locus_tag="VEP73_03185"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYT25472.1"
                     /translation="MSPAVEHDPADNLRAYAHPERLVTTAWLAGHAGDPGLVVAESDE
                     DVLLYETGHIPGAVKIDWHNDLNDDVIRDYVDGEGFARLMSSKGIARDTTIVFYGDKS
                     NWWAAYALWVATLFGHPDVRLLDGGRQKWIEEGRPLTTEVPRRAATEYPVVERDDKVI
                     RGFREDVLAHVATGGRLVDVRSPGEYSGELLAMPDYPQEGALRGGHIPGAANVPWKRA
                     ANDDGTFKSARELHAIYEGELGLTHADDVIAYCRIGERSSHTWFVLSQLLGFPRVRNY
                     DGSWVEWGNAVRVPIERSAEPRRLTPEVG"
BASE COUNT          189 a          581 c          558 g          226 t
ORIGIN      
        1 ggcgggcagc ggcggcaccc gccgcgagta gtcgagcagc gcctcgaggc gcagcgctcc
       61 gggcaggccg gcgaactcgt cgacgacctc ccggagccgg gccggcagcg tggtcgtcgt
      121 catccaacct ccggggtcag ccgccgcggc tcggcggacc gctcgatcgg cacccgcacg
      181 gcgttccccc actcgaccca ggagccgtcg tagttccgca cccgcgggaa gccgagcagc
      241 tgcgagagca cgaaccaggt gtggctcgac cgctcgccga tgcggcagta cgcgatcacg
      301 tcgtcggcgt gggtcaggcc gagctcgccc tcgtagatcg cgtggagctc ccgggccgac
      361 ttgaacgtgc cgtcgtcgtt cgctgcccgc ttccagggga cgttcgccgc acccgggatg
      421 tggccgccgc gcagcgcgcc ctcctgcggg tagtccggca tggcgagcag ctcgcccgag
      481 tactcgcctg gcgagcggac gtcgaccagg cgcccgccgg tggcgacgtg ggcgaggacg
      541 tcctcgcgga acccccggat caccttgtcg tcccgctcga cgaccgggta ctcggtcgcc
      601 gcccgccggg gcacctcggt ggtgagcggg cgcccttcct cgatccactt ctgcctgccc
      661 ccgtcgagga gccggacgtc gggatggccg aagagggtgg ccacccacag ggcgtaggcg
      721 gcccaccagt tcgacttgtc gccgtagaac acgatcgtgg tgtcgcgcgc gatccccttg
      781 gacgacatca gcctggcgaa gccctcgccg tcgacgtagt ccctgatcac gtcgtcgttc
      841 aggtcgttgt gccagtcgat cttgaccgcc cccgggatgt ggccggtctc gtagagcagc
      901 acgtcctcgt ccgactcggc caccaccagg ccggggtcgc cggcatgccc ggccagccac
      961 gcggtggtga ccagccgctc ggggtgggcg taggcacgca ggttgtcggc ggggtcgtgc
     1021 tccaccgcgg ggctcatcgg ctcctccttc tctgtccgct ctccgcctgc tcttcggtgt
     1081 cggcgcgagg cggcggccgt tgaggccggc acacccgctg ccgatacatg ccaccgtacg
     1141 cccgtgtcgg gaacgctgac cgtacgcccg ttccgggaac gctggaggcc gaccaggggt
     1201 cggccggcga gggggctcat ggcgctcgca gggtctggct cccgcgcgac gcgtaggcac
     1261 cctcccctgt atgcgggtcg acaagcgcct tggcgaggct ggaaggtgct cggcggcaga
     1321 atgacgcatc tcggcgctct cgctggctcc ctgccggcgg cgtcccgccg cagtcggaga
     1381 ggtggcgcat ggccgccggc ctaggaaccg ccacgcagga cgaccggcac gcaccccggc
     1441 ccccccggcg acgatggggc cgctgggccg ccctcgccgt cctgctcgtc gtggcggcgt
     1501 ccctcgcggc cggcgcctgg ctggcgctcg ggctcctcca cgccgcgagg gacg
//
LOCUS       DASPYY010000210         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2004801, whole genome shotgun sequence.
ACCESSION   DASPYY010000210 DASPYY010000000
VERSION     DASPYY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004801"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VEP73_03190"
     CDS             <1..939
                     /locus_tag="VEP73_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HYT25473.1"
                     /translation="GLRAASGRPPAAGVDLRNAGGAPVTIVLADSGQVIGTVDRARAT
                     STVHQGAVYLHQGEPYRVVELDLDGGAALVEAATGDEYTQARRDTDIGIVEATRRVDL
                     PRSSLWLGRVAVSDQVVGYERRRAGSGEVLGYEDLDLPPTLLPTVAYWWTLSTELLAA
                     ARLDPAEVPGAAHAAEHAQIGLLPLFAMCDRWDIGGVSTAWHPDTGMATIFVYDGHPG
                     GAGIAERGHQLAARHLRATRDAVHACPCETGCPSCVQSPKCGNGNHPLDKAGAVRLLD
                     ELLADLDAAPAATVAGDAVPATAAGPARELSHGPGR"
     gene            936..1421
                     /locus_tag="VEP73_03195"
     CDS             936..1421
                     /locus_tag="VEP73_03195"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HYT25474.1"
                     /translation="MTLYIGVAGASQPEPELAGMAEALGRRLAEAGAIVVCGGGGGVM
                     AAVCRGAVAAGGTTVGLLPGGDRSGGNPHLRIALPTGLGEGRNVLLVRASDALVAVGG
                     GYGTLSEIALALRTGVPVVGLRTWSLALAGRPVEAFPVVDRPDRAARLALDAAEARAT
                     P"
     gene            1572..2006
                     /locus_tag="VEP73_03200"
     CDS             1572..2006
                     /locus_tag="VEP73_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25475.1"
                     /translation="MAVFFLLVAVLAGAAIGDAIYENTGAGTLVIAQQTTDRFTQGEL
                     LAIFAGLGFLLALLLLLAVATAPRRRDRRRALRAARRDAEQRITELQRANARLQADVA
                     RRDQTLASLDAELTRGRRARAPSPVHVTDDDPTPPFGLARHA"
     gene            2071..>2514
                     /locus_tag="VEP73_03205"
     CDS             2071..>2514
                     /locus_tag="VEP73_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYT25476.1"
                     /translation="MLGRRALNRALLERQLLLRRWQLPAADAIERLVGMQAQVPDSPY
                     AGLWTRLDGFRPDDLARLITGRQAVRAPLMRTTLHLVTARDCLALRPVVQPVLERALR
                     TGSPFGRRIAGVDTEALLAAGRALLEEQPRTTAALGKLLHAQWPDR"
BASE COUNT          293 a         1031 c          902 g          288 t
ORIGIN      
        1 gggctgcggg ccgcgtccgg ccgcccgccc gccgccggcg tcgacctccg caacgccggc
       61 ggcgccccgg tcaccatcgt gctggccgac tccggccagg tgatcggcac cgtcgaccgg
      121 gcccgcgcga cctccaccgt ccaccagggc gcggtctacc tccaccaggg cgagccctac
      181 cgggtcgtcg aactcgacct ggacggcggc gccgccctcg tcgaggccgc caccggcgac
      241 gagtacaccc aggcccggcg cgacaccgac atcggcatcg tcgaggcgac ccgccgggtc
      301 gaccttcccc gctcgagcct gtggctcggc cgggtcgccg tctccgacca ggtcgtcggg
      361 tacgaacggc gccgggccgg gtcgggtgag gtcctcggct acgaggacct ggacctgccc
      421 ccgacgctcc tgccgaccgt cgcgtactgg tggaccctct cgaccgagct gctcgccgcg
      481 gcccgcctcg acccggcgga ggtgccggga gccgcccacg ccgccgagca cgcccagatc
      541 ggcctcctcc ccctgttcgc gatgtgcgac cgctgggaca tcgggggcgt gtcgaccgcc
      601 tggcaccccg acaccggcat ggccacgatc ttcgtctacg acggccaccc gggcggtgcc
      661 gggatcgccg agcgcggcca ccagctcgcg gccaggcacc tccgcgcgac ccgcgacgcc
      721 gtccacgcct gcccctgcga gaccggctgc ccgtcgtgcg tccagtcgcc caagtgcggc
      781 aacggcaacc acccgctcga caaggccgga gcggtgcgcc tgctcgacga gttgctcgcc
      841 gacctcgacg ccgccccggc ggccacggtc gccggcgacg ccgtcccggc cacggccgcc
      901 ggcccggccc gcgagctgtc gcacggaccg ggccggtgac cctctacatc ggcgtggcgg
      961 gcgcgtcgca acccgagccc gagctcgccg gcatggccga ggccctgggc cgccgcctcg
     1021 ccgaggccgg cgcgatcgtc gtctgcggcg ggggtggcgg cgtgatggcc gcggtctgcc
     1081 ggggagcggt cgcggccggc ggcaccacgg tcgggctgct gccgggcggc gaccggagcg
     1141 gcggcaaccc ccacctccgc atcgccctgc ccaccggtct cggcgaagga cgcaacgtcc
     1201 tgctggtcag ggcgagcgac gcgctcgtcg ccgtgggtgg cggctacggc acgctgtccg
     1261 agatcgcgct ggccctgcgg accggcgtgc cagtcgtcgg cctgcggacc tggtcgctcg
     1321 cgctcgccgg ccggcccgtc gaggccttcc ccgtcgtcga ccgaccagac cgggccgcgc
     1381 gcctggccct cgacgccgcc gaggcccgcg ccactccatg agcgggaccg gcgggcaggc
     1441 acggcaccgt cgcggcgccg gccacacggc ggccgttcgg cggcaggcac gacggcggtt
     1501 cggcggcagg cacccagggc atcccgcgcc gagcggggcg gcccgtccga gcacccggag
     1561 gcgagcaacg catggccgtg ttcttcctgc tggtcgctgt cctcgccggc gccgcgatcg
     1621 gcgacgcgat ctacgagaac accggcgccg gcaccctcgt catcgcccag cagacgaccg
     1681 accggttcac ccagggagaa ctcctcgcga tcttcgccgg tctcggcttc ctgctcgcac
     1741 tcctgctgct cctggccgtc gcgaccgcac ctcgccgcag agaccggcgc agggcgctgc
     1801 gggcggcacg gcgcgacgcc gagcagcgca tcaccgagct gcagcgcgcc aacgcgaggc
     1861 tccaggccga cgtcgcccgg cgcgaccaga ccctcgcgag cctggacgcc gagctcaccc
     1921 ggggccggcg cgcgcgtgct ccctcgcccg tccacgtgac cgacgacgac ccgacgcccc
     1981 cgttcggtct cgccaggcat gcctgacgcc acctcgccat cgccaggtcg agcatcgcct
     2041 cccgcacgag gcaggggcgg gccgggtgag gtgctcggac ggcgggcgct caaccgcgcc
     2101 ctgctggagc ggcagctgct gctccggcgc tggcagctgc ccgcggccga cgcgatcgag
     2161 cggctggtcg gcatgcaggc gcaggtgccc gactctccct atgccggcct gtggacgcgg
     2221 ctggacggct tccgcccgga cgacctggca cggctgatca ctggccggca ggcggtgcgc
     2281 gccccgctga tgcgcacgac gctgcacctg gtgaccgccc gcgactgcct ggcgctgcgc
     2341 ccggtggtgc agcccgtcct ggagcgcgcc ctgcgcacgg gcagcccctt cggccgacgc
     2401 atcgcgggag tcgacaccga ggcgctcctg gcggccggtc gggcgctgct ggaggaacag
     2461 ccgcgcacca ccgcggcgct cggcaagctg ctgcacgcgc agtggcccga ccgc
//
LOCUS       DASPYY010000211         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_37812, whole genome shotgun sequence.
ACCESSION   DASPYY010000211 DASPYY010000000
VERSION     DASPYY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37812"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VEP73_03210"
     CDS             <1..1962
                     /locus_tag="VEP73_03210"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HYT25477.1"
                     /translation="RPGFAVRDEAGAAAVAEVCRRLDGLPLAIELAAARVGALPPREL
                     AARLDDRFRLLTAGARTALPRQQTLRAVVDWSWELLDPPERAALRRLSVFVGGCTLDA
                     AERACSGPDLPAVDVAEAIARLIDKSLVTVGPTPAPPALWWPGIEALGLPALPEPEPA
                     GGTVRYGLLETVRAYAAERLAEAGEADRVARAHAEWCVWLAETAEPQLNGHEQLAWYA
                     RLGVELDNLRAAWHWLLDRGEASLAVRLLGSLGFFLLMTGRREDAIGALERALALPGP
                     VPDRARGQALYVLTIGADAVDDRDALHRVAKEAQAALERAAADPAEAAFARTVAATLG
                     FFFTGGALEAEQAIDEALEEVERAGGWLAALLRQLRGFARAMRGNLAGAIEDGEACLE
                     RFRELGDRWGIVQALELLGMVDTVQGRYELAEARIGEGLRCAWELRVPAELAVQLCRF
                     AYLAMARGDLDRAEARLEQALAAAQEFGVGGAAAFAHAGLGPVAQRRGDLERAGRECQ
                     QALTLLQRHGARSPVGPLLLSWLGTVALQQGDLERAAALHGEVLEWSLRHGGSPSAVA
                     GALVGSAAVIRAQGDPERAAVLLGAAAAARRSAGVPLPPGDRVDVDHTAAEALEALGE
                     ERFARALARGEALPLEEAVALVGQLAEEG"
BASE COUNT          195 a          720 c          824 g          245 t
ORIGIN      
        1 cgccccgggt tcgcggtccg tgacgaggcc ggcgccgccg cggtggcgga ggtgtgccgc
       61 cggctcgacg ggctgccgct ggcgatcgag ctcgccgccg cgcgggtcgg cgcgctgccg
      121 ccgcgcgagc tggccgcgcg gctggacgac cgcttccggc tgctcaccgc gggcgcgcgg
      181 accgcgttgc cgcgccagca gaccctgcgg gcggtggtgg actggagctg ggagctgctc
      241 gacccgcccg agcgtgccgc cctgcggcgc ctctcggtgt tcgtgggcgg ctgcaccctc
      301 gacgccgccg agcgggcctg ctcgggacct gacctgcccg ccgtggacgt cgcggaggcg
      361 atcgccaggc tgatcgacaa gtccctggtc accgtggggc ccaccccggc gccgccggcg
      421 ctgtggtggc cgggcatcga ggcgctcggg ctgccggcgc tgcccgagcc tgagcccgcc
      481 ggcggcacgg tccgctacgg cctgctggag acggtgcgtg cctacgccgc ggaacggctc
      541 gccgaggcgg gcgaggcgga ccgggtggca cgcgcccacg ccgagtggtg cgtgtggttg
      601 gcggagacgg ccgagccgca gctcaacggg cacgagcagc tcgcctggta cgcccggctc
      661 ggcgtggagc tcgacaacct gcgggccgcg tggcactggc tgctcgaccg cggcgaggcc
      721 tcgctcgccg tgcggctgct cgggtcgctg ggcttcttcc tgctcatgac cggtcgtcgt
      781 gaggacgcca tcggcgcgct cgagcgtgcg ctcgccctgc cgggaccggt gccggaccgg
      841 gcacgcggcc aggcgctgta cgtcctcacc atcggggcgg atgcggtgga cgaccgtgac
      901 gccctccatc gcgtggccaa agaagcccag gcagccctcg agcgcgccgc ggcggatccc
      961 gcggaggccg cgttcgcgcg gacggtggcg gcgacgctcg gcttcttctt caccggcggt
     1021 gccctcgagg ccgagcaggc catcgacgag gccctcgagg aggtggagcg ggccgggggc
     1081 tggctcgccg cgctcctgcg ccagctgcgg gggttcgccc gggccatgcg cgggaacctg
     1141 gccggcgcca tcgaggacgg cgaggcgtgc ctggagcgat tccgcgagct cggcgaccgc
     1201 tgggggatcg tgcaggcact ggagctgctc ggcatggtcg acaccgtgca gggccgctac
     1261 gagctggccg aggcgaggat cggggagggc ctgcgctgcg cctgggagct gcgcgtgccc
     1321 gcggagctgg cggtgcagct gtgccggttt gcctacctgg cgatggcacg gggcgacctg
     1381 gaccgggccg aggcgcggct cgagcaggcg ctcgccgccg cccaggagtt cggggtcggg
     1441 ggtgcggccg ccttcgcgca cgccggcctc ggcccggtgg cacagcgacg cggcgacctc
     1501 gagcgcgccg ggcgggagtg ccagcaggcc ctgacgctgc tgcaacgcca cggtgcacgc
     1561 agccccgtcg ggccgctcct gctgagctgg ctcgggaccg ttgccctgca gcagggcgac
     1621 ctggagcgcg ccgcggccct ccatggcgag gtgctggagt ggtcccttcg gcacggcggc
     1681 agcccgtcgg ccgtcgccgg ggcgctggtg gggtccgcgg cggtgatccg cgcccagggt
     1741 gacccggagc gggccgcggt gctgctgggc gcggccgcgg cggcgcgccg ctcggcgggc
     1801 gtgccgctgc ctcccgggga ccgcgtggac gtcgaccaca ccgccgccga ggcgctcgag
     1861 gcgctggggg aggagcgctt cgcccgggcg ctcgcccgcg gcgaggccct gccgctggag
     1921 gaggccgtcg cgctcgtcgg gcagctcgcc gaggagggct gagcgcccgg tccaggcagg
     1981 gccg
//
LOCUS       DASPYY010000212         8768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1162972, whole genome shotgun sequence.
ACCESSION   DASPYY010000212 DASPYY010000000
VERSION     DASPYY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162972"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VEP73_03215"
     CDS             <1..708
                     /locus_tag="VEP73_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HYT25478.1"
                     /translation="DLAMLSDIFPTGYHGAVSAGVTTGSTVYVAGAGPVGLACATSCH
                     LLGAAVVIVGDMNADRLRQAASFGCETVDLSATDDLRGALQQILGGSGEVDSAVDCVG
                     FEARGHGKEGSQGEAPATVLNSIMDITRAAGKLGIPGLYVTGDPGAVDEPAKVGSLSI
                     RIGLGWAKAHSLVTGQCPVMRYNRELMTAILHDKVQIAKNVNATVIPLDRAPQGYQDF
                     DKGAARKYVIDPHGMVA"
     gene            complement(803..1159)
                     /locus_tag="VEP73_03220"
     CDS             complement(803..1159)
                     /locus_tag="VEP73_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25479.1"
                     /translation="MGRRARARERAVAPATEATAVGRGLASRAADGRAAAGTSRGRRV
                     LGILNPLKATSRRRMAWAAVVFAVLGLALVVAGVATGRPEWYRPALLLGLLAVVWGAR
                     AALTAEAERRPASRRP"
     gene            1233..2357
                     /locus_tag="VEP73_03225"
     CDS             1233..2357
                     /locus_tag="VEP73_03225"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYT25480.1"
                     /translation="MRIVVAPDKFEGSLTADEAARAIEAGLLRARPDAEVVRLPVGDG
                     GAGTLAAVLAAGFERVPVRATGPTGEPVEAAIAVQGDSAFVEMAEASGLRRLPGGRRA
                     PRDATTYGTGELVRAALDRGARTLVLGIGGSATTDGGTGMASALGARFLDGAGRELPP
                     GGAALLRLARIDTAGLDPRIAEVEVTVASDVDNPLVGPHGAAAVYGPQKGASPDDVLL
                     LDSALRRYARVLAADLGMDLAATPGAGAAGGLGAGAIAFLGATLEPGIELVLGLVGFD
                     RAVAKADLVVTGEGKIDAQSLRGKAPVGVAAAATAQGVPVVALGGVVEVSARELRAAG
                     FEEAHGLTELEPDVERCIAEAAPLLERLAERVGRAWSSLP"
     gene            2471..2977
                     /locus_tag="VEP73_03230"
     CDS             2471..2977
                     /locus_tag="VEP73_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYT25481.1"
                     /translation="MTDDALPRAASAVLEAGRGLGLEMDVREFPEGTRTAADAASAIG
                     CAVDQIVKSLVFVADNTPVVVLTSGVNQVDVVKVAKETGTVTVRRADAGEARAATGYA
                     IGGTPPFGHARRLEVLVDPHLGRFDTVWAAAGTPRHVFPIPAGELARVTGGRVADITL
                     DDIGPKAD"
     gene            3116..3466
                     /locus_tag="VEP73_03235"
     CDS             3116..3466
                     /locus_tag="VEP73_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYT25482.1"
                     /translation="MSEVTETATSVVDLTEGAATKVRDLLTREGRDDLALRIAVEPGG
                     CSGLRYALFFDDRSLDGDVRGQVHGVQVVTDRMSAPYLTGASIDYLDTLEKSGFTIDN
                     PNAQGSCACGESFH"
     gene            complement(3608..3859)
                     /locus_tag="VEP73_03240"
     CDS             complement(3608..3859)
                     /locus_tag="VEP73_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018804537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HYT25483.1"
                     /translation="MTAAGAPPPAEVVDCLGLSCPLPLVRLAKAVAGVDVGTDVVLLS
                     DDPGALVDVPVWCRLKDHELLGREDRPEGGWSFRVRRGS"
     gene            4126..4785
                     /locus_tag="VEP73_03245"
     CDS             4126..4785
                     /locus_tag="VEP73_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25484.1"
                     /translation="MDRKDDAYRRILEWDMPERGDTSAAPAISWAVGLALPVALAAFL
                     VGWLALGWTAHRQAIVWLAALAGPAVGLAVVGVRAGRGPWQRLRTALLAVGAAVLVMA
                     WVVGLLLGSSRPTTVELRSELDHLGVPYASVPGSEVAEGNRFCRRFCVSVARDFRVPR
                     SGQENQVTMFIDALVRAGWKVPPGVRQADTTEVLRGGVKASVGGGVAGGDVQVELTSA
                     G"
     gene            complement(4844..5773)
                     /locus_tag="VEP73_03250"
     CDS             complement(4844..5773)
                     /locus_tag="VEP73_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT25485.1"
                     /translation="MRHMERRWSVAAVLAALSVVLAACGGLSKSPGSGSGGSGKLEVR
                     LGVVDFTEQVILGQIYGRALARAGYKVTYQKLASRELADPALFAGQLDMLIEYAGSDL
                     TYLKGTPTPDGRQILTDLKAKLQPKNVTVLEQAPASDQQAITMTQASSDRYEAKTLSD
                     LAKVAGQLVFGAPPECFQNRGTCYKGMQETYRINFKATRSIPNASVRHQALLGNQIQA
                     NLSFSTDGVIARQKLVVLQDDKKLFPPDHVVPIARNDFLSKAGGDLQSTVDKVSAAIT
                     TDGLTALNAKVDLDQEDPDRVATEWLREKNLLA"
     gene            6055..6252
                     /locus_tag="VEP73_03255"
     CDS             6055..6252
                     /locus_tag="VEP73_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25486.1"
                     /translation="MASKGRAVQGARTQPAVTKGGQKTFAVGRACPTCGEPLSQYNPG
                     PNCFKHTVGFPWRGPTAKPKY"
     gene            6288..6995
                     /locus_tag="VEP73_03260"
     CDS             6288..6995
                     /locus_tag="VEP73_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYT25487.1"
                     /translation="MPDASQLDDLDPFELLATERARLDRFFSGLDEAGWDRPTRCTGW
                     RAREMIAHLAGGELYNQACLDDKLGELFERAGRAGAEGFQGFTDWTVSERAGRPPTEV
                     LDEWRRASSDTLRRLREHGRDGTIPTSAGPYPAGLQAFHLAIEDATHADDLGAPVDPD
                     EAADRLAWRATVSRFGVTETGKPVELEARGDRMLVRSGGQEAALSQDELVQAAVARLD
                     ADHPLPAGLREALRAFA"
     gene            complement(7072..8208)
                     /locus_tag="VEP73_03265"
     CDS             complement(7072..8208)
                     /locus_tag="VEP73_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYT25488.1"
                     /translation="MPLPRSPRPAAAALLAGLLAAALLAAAPAGAAPRPVPGDGAIDR
                     ARAEAARVQRSIDRLDVEVELLAEAYDANAERLDAVIRASLVHQRELADGEQALEAAR
                     AGFDDQVRSLYIEGPAASLELLLVAQDGHELAVARRAVDHSLDARRQAVAGVRAAADR
                     LGARVAELQAAQAEVIGIRGRLADQRAGIERRLGLQRRLLADAERHVRLLVAEARARE
                     EAARRAALATAAARARVLGLEGFATAPPPTAAAATAVGAALSQVGKPYRWGGTGPDDF
                     DCSGLVGWAWARAGTALPRTSRQQWSAGPHVDPAHLLPGDLVFFGSVPGDPASIHHVG
                     MYVGQGLMVDAPHTGAQVRVESIRAGGYVGATRPGAGRPAPAAR"
     gene            8362..>8768
                     /locus_tag="VEP73_03270"
     CDS             8362..>8768
                     /locus_tag="VEP73_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYT25489.1"
                     /translation="MNCSSCGHPSPAGNRFCGSCGAELDHAGTLSAAAAAPAPSPAMA
                     ASPHPAAAGWSPAPPPARRRAGPALLAALMGLLLLFGGVGLFAARSVTARAGAGSPEA
                     AASGLLGALDKQDLERAAGYLDGEERQAVTVYA"
BASE COUNT         1058 a         3291 c         3309 g         1110 t
ORIGIN      
        1 gacctggcga tgctgtcgga catcttcccg accggctacc acggcgcggt cagcgccggg
       61 gtgaccaccg ggtccaccgt ctacgtcgcc ggcgccgggc ccgtcgggct cgcctgcgcg
      121 acctcctgcc acctgctcgg ggccgcggtg gtgatcgtcg gcgacatgaa cgccgaccgg
      181 ctgcggcagg ccgcgtcgtt cggctgcgag acggtcgacc tgtcggcgac cgacgacctg
      241 cgcggcgcgc tgcagcagat cctcggcggc tccggcgagg tggactccgc ggtcgactgc
      301 gtcgggttcg aggcgcgcgg ccacggcaag gagggctcgc agggcgaggc gccggcgacc
      361 gtgctcaact cgatcatgga catcacccgg gcggccggga agctcggcat cccgggcctg
      421 tacgtcaccg gcgaccccgg cgcggtcgac gagcccgcca aggtcggctc gctgtcgatc
      481 cggatcgggc tcggctgggc caaggcccac agcctcgtga ccggccagtg cccggtgatg
      541 cgctacaacc gcgagctgat gacggcgatc ctgcacgaca aggtgcagat cgcgaagaac
      601 gtcaacgcga ccgtgatccc gctcgaccgg gccccgcagg ggtaccagga cttcgacaag
      661 ggcgcggcca ggaagtacgt gatcgacccg cacggcatgg tcgcctgacc cctccgggtg
      721 ggagggagcc gtccggctct ctcccacctc acccggccgg gcggtcgcct ccgcgcggct
      781 ccccggcccg gctcgccgcg gctcagggac ggcggctggc cggccggcgt tcggcctccg
      841 cggtgagggc cgcgcgagcg ccccagacca ccgccaggag cccgagcagc agggccgggc
      901 ggtaccactc gggccgcccg gtcgcgaccc cggcgaccac gagcgcgagg ccgagcacgg
      961 cgaacaccac cgcggcccag gccatccgcc tccgggaggt ggccttcagc gggttcagga
     1021 tgccgaggac ccggcgcccg cgggacgtgc ccgccgcggc ccggccgtcc gcggcccgcg
     1081 acgccaggcc gcggcccacc gccgtcgcct cggtggcggg ggccaccgcc cgctcgcgtg
     1141 cccgtgcccg acgtcccatc gcagcctccg cgccgcccgg catccacgtc cgagacggga
     1201 tgctgcttcc tcgcgttggt atggtacggc ccatgaggat cgtcgtggcg cccgacaagt
     1261 tcgagggcag cctgaccgcc gacgaggcgg ccagggcgat cgaggccggc ctgctgcgcg
     1321 cccggcccga cgccgaggtc gtccgtctgc ccgtgggcga cggcggcgcc gggacgctcg
     1381 ccgccgtgct cgccgccggg ttcgagcggg tgcccgtccg ggccaccggc cccaccggcg
     1441 agccggtcga ggccgccatc gccgtccaag gcgacagcgc cttcgtcgag atggccgagg
     1501 cctccggcct ccgccgcctg cccgggggcc gccgggcgcc cagggacgcc accacctacg
     1561 gcaccggcga gctcgtccgg gccgccctgg accggggcgc ccggacgctc gtgctcggca
     1621 tcggcggcag cgccaccacc gacggcggga cggggatggc gagcgcgctc ggcgcccgct
     1681 tcctcgacgg cgccgggcgc gagctgccgc caggcggcgc ggcgctcctg cggctcgcgc
     1741 gcatcgacac ggccggcctg gacccgcgca tcgccgaggt cgaggtcacg gtcgcctccg
     1801 acgtcgacaa cccgctggtc ggcccccacg gcgccgccgc ggtctacggg ccccagaagg
     1861 gcgccagccc cgacgacgtg ctgctgctcg actcggcgct gcgccgctac gcccgcgtgc
     1921 tcgctgccga cctcgggatg gacctggccg ccacgccggg cgcgggtgcg gcgggcggcc
     1981 tcggggccgg ggcgatcgcg ttcctcggcg ccaccctcga gcccgggatc gagctggtgc
     2041 tcggcctcgt cggcttcgac cgcgcggtgg ccaaggccga cctcgtcgtc accggcgagg
     2101 gcaagatcga cgcccagagc ctgcgcggca aggccccggt cggggtcgcc gcggcagcga
     2161 ccgcgcaggg cgtgcccgtg gtcgcgctcg gcggcgtcgt cgaggtgtcc gcgcgggagc
     2221 tgcgcgccgc gggcttcgag gaggcgcacg ggctcaccga gctcgagccg gacgtcgagc
     2281 ggtgcatcgc cgaggcggcg ccgctgctcg agcgcctggc cgagcgggtc ggccgggcct
     2341 ggtcgtcgct gccgtagtac ccggcctcgc gtaccatgcc cacccgatcg ttcccacccg
     2401 atcatgatgc ctacccgggg gccgtgcccg cgccgcccga cctccggcac gcgagagaag
     2461 gttgacgccc atgacggacg acgccctgcc cagagcggcc tccgccgtcc tcgaggccgg
     2521 caggggcctc ggcctcgaga tggacgtgcg cgagttcccc gagggcaccc ggaccgcggc
     2581 cgacgcggcc agcgccatcg gctgcgcggt cgaccagatc gtgaagtcgc tcgtgttcgt
     2641 agccgacaac accccggtcg tggtgctcac cagcggggtc aaccaggtgg acgtcgtcaa
     2701 ggtggccaag gagaccggca cggtcacggt gcgcagggcc gacgccggcg aggccagggc
     2761 cgcgaccggc tacgcgatcg gcggcacccc gccgttcggc cacgcgcgcc gcctcgaggt
     2821 gctcgtcgac ccccacctgg gccggttcga cacggtctgg gccgcggccg ggaccccgcg
     2881 ccacgtcttc ccgatcccgg ccggggagct ggcccgggtg accggcggcc gggtggccga
     2941 catcaccctg gacgacatcg ggcccaaggc ggactgaccg gggtccgggg ctcgaggagc
     3001 gaagccgagt ggtcggaggt cgagtgcgga aggttggccc cgtcggcgct atcatttggg
     3061 tacacgaacg ttgaagaaac gatctcccgg ccctgccgga gcgaaaggag cacggatgtc
     3121 cgaggtcacc gagacggcga cgtcggtcgt ggacctgacc gagggtgcgg ccaccaaggt
     3181 tcgcgacctg ctcacacgcg aggggcgtga cgacctcgcg ctgcggatcg cggtcgagcc
     3241 cggcggctgc tccggcctcc gctacgcgct cttcttcgac gaccggtcgc tcgacggcga
     3301 cgtgcgcggc caggtccacg gcgtgcaggt ggtcaccgac cgcatgagcg ccccgtacct
     3361 gaccggcgcg tccatcgact acctggacac cctcgagaag tccgggttca cgatcgacaa
     3421 tcctaacgcc cagggcagct gtgcctgtgg cgagtcgttc cactgacgta gtcctcgttc
     3481 cacgtggggg gagctcgctc cccccacggc cccccaaggg cgcctgtgcc atggtcgggc
     3541 cgatctccct tccgccacgg ccctcaaggg cgcctgtgcc atggtgcggt gatcccgcca
     3601 gcctccgcta gcttccgcgg cggacgcgga aggaccagcc gccttctggg cggtcctcac
     3661 ggcccaggag ctcgtggtcc ttcagccggc accagacggg gacgtcgacg agggcgccgg
     3721 ggtcgtcgct gagcagcacg acgtcggtgc cgacgtcgac cccggcgacg gccttggcca
     3781 gccggaccag cgggagcggg caggagagcc cgaggcagtc gaccacctcg gccggcggcg
     3841 gcgctccggc ggcggtcacc ggccgccccc cgcccacccg atcgccggcg catgcggcgc
     3901 ggcgtcgagg tacagctcct ggcaggactc gtcgatcgtg gtcaccgtca atcgctcgga
     3961 ggcgcccggg tcggcatcgc ccgtccaggg tagcggcgac cggggcgcgg ccggcgggcc
     4021 accgggtacc ggcggctcca cctccggcga aatcgcttgc ggtggcgtcg gggggaggga
     4081 gcatgcttgg gcgagtcgcg acgagcccac cgtgggggtc tgcaagtgga ccgcaaggac
     4141 gacgcatatc gccggatcct ggaatgggac atgcccgagc ggggcgacac gagcgccgcc
     4201 ccggccatct cctgggcggt cggtctcgcg ctgcccgtgg cgctcgccgc cttcctggtc
     4261 ggctggctcg cgctcggctg gacggcgcac cggcaggcca tcgtctggct cgccgcgctg
     4321 gccggcccgg cggtcggcct cgccgtcgtc ggcgtgcggg cgggccgggg cccgtggcag
     4381 cggctccgca ccgccctcct ggccgtcggg gcggcggtcc tggtcatggc gtgggtggtc
     4441 gggctcctgc tcggctcctc caggcccacg accgtggagc tgcggtcgga gctcgaccac
     4501 ctcggcgtcc cctacgcgtc ggtgcccggg agcgaggtag ccgagggcaa ccgcttctgc
     4561 cggcgcttct gcgtgtcggt ggcccgcgac ttccgcgtac cgaggagcgg gcaggagaac
     4621 caggtgacga tgttcatcga cgcgctcgtc cgggcgggct ggaaggtccc tcccggcgtc
     4681 cggcaggccg acacgaccga ggtgctgcgg ggcggggtga aggcgagcgt cggcggcggc
     4741 gtggccggcg gggacgtgca ggtcgagctg acctccgcgg gctgagacgc gcggcccacc
     4801 ccggccgcgg ggccgtggtg cggcccgggc ggcctcgccg cggtcaagcg agcaggttct
     4861 tctccctcag ccactcggtc gccacgcggt ccgggtcctc ctggtcgagg tccaccttgg
     4921 cgttcagcgc ggtgaggccg tcggtggtga tcgcggcgct gaccttgtcc acggtggact
     4981 ggaggtcgcc gcccgccttg ctgaggaagt cgttgcgggc gatcggaacg acatggtcgg
     5041 gcgggaagag cttcttgtcg tcctggagga cgacgagctt ctgcctcgcg atgacgccgt
     5101 cggtcgagaa ggacaggttg gcctggatct ggttgccgag cagggcctgg tgcctgaccg
     5161 aggcattggg gatcgacctg gtggccttga agttgatcct gtaggtctcc tgcatgccct
     5221 tgtagcaggt cccgcggttc tggaagcact cgggcggcgc gccgaacacc agctggcccg
     5281 cgaccttggc gaggtcggac agcgtcttcg cctcgtacct gtccgagctc gcctgggtca
     5341 tcgtgatggc ctgctggtcg cttgctggcg cctgctcgag gacggtcacg ttcttcggct
     5401 gcagcttggc cttgaggtcg gtcaggatct gccggccgtc gggggtcggg gtgcccttca
     5461 ggtaggtgag gtcggacccg gcgtattcga tcagcatgtc gagctggccg gcgaacaggg
     5521 ccgggtcggc gagctcccgg ctggcgagct tctggtaggt caccttgtag cccgccctgg
     5581 cgagcgcccg gccgtagatc tggccgagga tgacctgctc ggtgaagtcg accacgccga
     5641 gccggacctc cagcttcccg gagccgcccg agcccgaacc agggctcttg ctgaggccac
     5701 cgcaggcggc gaggacgacc gagagcgcgg cgagcacggc cgcgacgctc cagcgacgtt
     5761 ccatgtgacg catgtgacgc ctgctcctgt cccgtcgacg atgtcccttc gacgatgaag
     5821 gtgccgagcc cgccgctcat accggccatg gtcgcacaac tcccccgcgc cgcagcgggc
     5881 gggtcggccc gcggcccggc tgagctgccc gaacgacaca cggcactttg cgtttcgagc
     5941 ccgccgggtg caacactcgc acctgcggtg ccgcgaccgg gctgccggac ggcgccctcg
     6001 cgcccgtagc cggcttcgga tccgccgtgc gagcggacaa aggaatggga tcacatggct
     6061 tccaagggtc gcgccgtgca gggcgcccgg acccagcccg ccgtcacgaa gggcgggcag
     6121 aagacgttcg cggtggggcg cgcctgcccg acgtgcggcg agccgctgtc gcagtacaat
     6181 cccggcccga actgcttcaa gcacaccgtc ggcttcccct ggcgggggcc gacggccaag
     6241 cccaagtact gactgaccgg ccgccggcac caggggggag cgccgagatg cccgacgcgt
     6301 cacagttgga cgacctcgac ccattcgagc tgctcgcgac tgagcgggcg aggctcgacc
     6361 ggttcttctc cgggctcgac gaggcggggt gggaccggcc cacccgctgc acggggtggc
     6421 gggcccggga gatgatcgcg cacctggccg gcggcgagct gtacaaccag gcgtgcctgg
     6481 acgacaagct gggcgagctg ttcgagcggg ccggcagggc cggtgccgag ggcttccagg
     6541 gcttcaccga ctggacggtc agcgagcggg cgggccggcc gcccaccgag gtcctcgacg
     6601 agtggcgccg ggcgtcgtcg gacaccctcc ggcgcctgcg cgagcacggg cgggacggca
     6661 cgatcccgac ctcggccggg ccgtacccgg ccggcctgca ggcgttccac ctcgcgatcg
     6721 aggacgccac ccacgccgac gacctcggcg cgccggtcga cccggacgag gcggcggacc
     6781 ggctcgcctg gcgagccacg gtcagccgct tcggggtgac cgagaccggc aagccggtcg
     6841 agctggaggc tcggggtgac cggatgctcg tccgctccgg cgggcaggag gccgcgctct
     6901 cgcaggacga gctcgtccag gcggcggtcg cccgcctgga cgccgaccac ccgctcccgg
     6961 ccggcctgcg cgaggccctg cgcgccttcg cctgatctct gacccgccgc cgggcgccgc
     7021 accgacgggc cgggccaggg gcccctcggc cggctaccct ccggcccagg gctagcgtgc
     7081 ggcgggggcc ggcctgccgg cgcccgggcg ggtggcgccg acgtagccgc ccgcccggat
     7141 cgattcgacc ctgacttgcg cgcccgtgtg cggcgcgtcg accatcagcc cctggccgac
     7201 gtacatgccg acgtggtgga tggaggccgg gtcgccgggg acgctgccga agaacaccag
     7261 gtcgccgggg agcaggtgtg cggggtcgac gtgggggccg gccgaccact gttgccgcga
     7321 ggtgcgcggc agcgccgtcc cggcccgggc ccaggcccag ccgacgaggc cggagcagtc
     7381 gaagtcgtcg gggccggtcc ctccccagcg gtacggcttg cccacctggc tgagcgcggc
     7441 acccacggcg gtggccgccg ccgccgtcgg cggcggcgcg gtcgcgaagc cctccaggcc
     7501 gaggacacgc gccctggcgg cagccgtggc gagcgcggcc cgtctggccg cttcctccct
     7561 ggcgcgggcc tcggccacga gcagccggac gtgccgctcg gcatccgcga gcagtcgccg
     7621 ctgcaggccg aggcggcgct cgatcccggc gcgctggtcg gcgagccgcc cgcggatgcc
     7681 gatgacctcg gcctgggcgg cctggagctc ggcgaccctg gcgccgagcc ggtcggcggc
     7741 ggcccgcacg ccggccacgg cctgccggcg cgcgtccagg gagtggtcga cggcccgtct
     7801 cgccacggcc agctcgtgcc cgtcctgggc gacgagcagc agctcgagcg aggccgccgg
     7861 cccctcgatg tagagcgagc ggacctggtc gtcgaagccc gctctggcgg cctcgagcgc
     7921 ctgctcgccg tcggccagct cccgctggtg gacgagcgac gccctgatga ccgcgtcgag
     7981 gcgctcggcg ttggcatcgt aggcctcggc gaggagctcc acctccacgt cgagccggtc
     8041 gatggagcgc tggacgcgcg cggcctccgc ccgcgcccgg tcgatggccc cgtcgcccgg
     8101 caccggtcgg ggagcggccc cggccggcgc cgcggcgagc agggccgcgg cgagcagccc
     8161 ggcgagcagg gccgcggcgg cgggacgcgg ggaccggggg agtggcacgg gagacctccg
     8221 atgcgagcac gtacgctccc gcgggttgtc ggcgccgcga cccgcccgct tgaccggcgc
     8281 gagccggccg cgcatgaccg gctcgccggt tatccttggg aggcggcacc gacggccgcg
     8341 tctcgcgttg ggagcgtcac catgaactgc tcgtcctgcg gccacccgtc cccggccggc
     8401 aaccggttct gcggctcgtg cggggccgag ctggaccatg ccggcaccct ttccgcggcc
     8461 gccgccgcgc ccgcgccgtc cccagccatg gcggcgtccc cgcatcccgc cgccgccggg
     8521 tggtcgcccg cgccgccacc cgcccgccgc cgcgccggac ccgcgctcct cgcggcgctc
     8581 atgggcctgt tgctgctgtt cggcggtgtc ggcctgttcg ccgcccgcag cgtcaccgcc
     8641 cgcgcgggcg ccggctcgcc cgaggccgcc gcctccggcc tcctcggcgc cctcgacaag
     8701 caggacctcg agcgggccgc gggttacctg gacggcgagg agcgccaggc cgtgaccgtc
     8761 tacgccag
//
LOCUS       DASPYY010000213         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1163096, whole genome shotgun sequence.
ACCESSION   DASPYY010000213 DASPYY010000000
VERSION     DASPYY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163096"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VEP73_03275"
     CDS             <1..339
                     /locus_tag="VEP73_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYT25490.1"
                     /translation="AAGSKQQDLAYDWLKLLAGEKYQTRLAKENGVLPNSSTLAESVL
                     GDNPVLSAMAKGAQNGRITPNDPTWAAVEAAPNPLKDMMTRVLSGKASVQDAAMEASG
                     QITQKMASAK"
     gene            350..1336
                     /locus_tag="VEP73_03280"
     CDS             350..1336
                     /locus_tag="VEP73_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYT25491.1"
                     /translation="MRPSGGGHLTAGAGHAPAPAAAAAAGSRREGPRRRGWTLPYLLV
                     LPSVVAFAALLGYPLYQVVRISLLKLDLGELIQRKTVFVGLANYRAIFADPFFWSVVV
                     RTVAFTATCVALTMVLGTCVALLLERLGTRMRLAVQLSLVLTWAMPPLTATVVWQWLF
                     DTQFGVVNWLLTKLGVGDFSNHSWFETGLSAFAVIAIIVVWQAIPFVAITLYAGLTAI
                     PRELREAMRVDGAGPWRTFRSLILPVLKPLFLITAFLSIIWDFKVFTQVWLVRQGGPD
                     RQTVTLALYAYQQGIATSHFGRAAAISLVMVVMLLVALVYYIRVMLDAQEDV"
     gene            1333..2157
                     /locus_tag="VEP73_03285"
     CDS             1333..2157
                     /locus_tag="VEP73_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYT25492.1"
                     /translation="MRRTRNALVNLAGAVTFLVFAFPVYWMFSTAFKPERDILTYTPH
                     PVPWPMTLQHFRDAVARPHFLDYVRNSVLVTSSAVLLSLAIATLAALAVGRFDFRGRK
                     AYLLLVMVVQMAPFEALLIPFFLMLRQLDLLNRLPALVLIYFIFTMPFTIWTLRGFVM
                     AVPRELEEAAMVDGCSRLQAFMRVVFPLLAPGMVATSIFAFVTAWNEFLYAFVFMRDP
                     DKLTLPVWLGTFRTAFGDDWGGTMAASALFTLPVLAFFLAIHRRLASGLTAGAVKG"
     gene            2157..>3489
                     /locus_tag="VEP73_03290"
     CDS             2157..>3489
                     /locus_tag="VEP73_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HYT25493.1"
                     /translation="MGSLQRLADACLLPAFEGTTAPDWVRRRVAEGLGGVVLYARNVA
                     SPAQVERLTAALRAERPALLVATDEEGGDVTRLEAATGSSYPGNLALGAAGDPALTRA
                     VGAALGADLAAAGISLDFAPVADVNSNPANPVIGVRSFGADPGRVAAHVVAMVEGLQG
                     AGVAACAKHFPGHGDTAVDSHLELPTAGGDAATLAGALVPFRAAVAAGVRCVMTAHVR
                     VPAYGEDPATLDRRVLTGLLREELGFDGLVVTDALEMRAIAGTVGFGEGGVRALTAGA
                     DALCIGGGHTGEGTVDLLRDAVVDAVRAGRLAEERLAEAAARVARVGTWAAGFRAEPG
                     AGPGAGPPAGRPGPRRGDPEAGLDAARRAVRAEGSVRVGTGAVVVELRPGASIAVGNV
                     PWGVGDLLASRDPTVEVIRCAEGGRVEGHPPDAAALAARAAGRPLVLVVRDL"
BASE COUNT          404 a         1340 c         1217 g          528 t
ORIGIN      
        1 gcggccggct cgaagcagca ggacctggcg tacgactggc tcaagctgct cgccggcgag
       61 aagtaccaga cccggctggc caaggagaac ggcgtgctgc cgaactcctc caccctggcc
      121 gagagcgtgc tcggcgacaa cccggtgctc agcgccatgg ccaagggcgc gcagaacggc
      181 aggatcaccc ccaacgaccc cacctgggcg gcggtcgagg cggcgccgaa cccgctgaag
      241 gacatgatga ccagggtcct gtcgggcaag gccagcgtcc aggacgcggc aatggaggcc
      301 agcggccaga tcacccagaa gatggcctcc gccaagtagg gtcctgcaca tgaggccgag
      361 cggtggcggg cacctgaccg ccggggccgg gcacgcgccc gccccggcgg cggccgccgc
      421 cgccggctcg cgaagggaag ggccgaggag acgcggctgg accctgccct acctgctcgt
      481 cctgcccagc gtcgtcgcgt tcgcggccct gctcggctac ccgctgtacc aggtcgtcag
      541 gatctcgctg ctcaagctcg acctcggcga gctcatccag cggaagacgg tcttcgtcgg
      601 gctcgccaac taccgggcga tcttcgccga ccccttcttc tggtcggtgg tcgtgcgcac
      661 ggtggcgttc accgccacct gcgtcgcgct caccatggtc ctcgggacct gcgtcgcgct
      721 gctgctcgag cggctcggca cccggatgcg gctcgcggtc cagctgagcc tcgtgctcac
      781 ctgggccatg ccgccgctca cggcgaccgt ggtgtggcag tggctgttcg acacgcagtt
      841 cggggtggtc aactggctgc tcacgaagct cggggtcggc gacttctcca accactcctg
      901 gttcgagacc ggcctgtcgg cctttgccgt catcgccatc atcgtcgtct ggcaggcgat
      961 cccgttcgtc gccatcacgc tgtacgccgg cctgactgcc atcccgcgcg agctgcgcga
     1021 agccatgcgg gtcgacggcg ccggcccctg gcggaccttc cggtcgctga tcctgccggt
     1081 gctcaagccg ctgttcctga tcaccgcgtt cctgtcgatc atctgggact tcaaggtctt
     1141 cacccaggtc tggctcgtcc gccagggcgg gccggaccgc cagacggtga cgctcgccct
     1201 ctacgcctac cagcagggca tcgccaccag ccacttcggc cgggccgcgg cgatctcgct
     1261 cgtcatggtc gtgatgctgc tcgtcgcgct ggtgtactac atccgcgtca tgctcgacgc
     1321 ccaggaggac gtgtgaggcg cacccggaac gcgctcgtca acctggcggg cgccgtcacc
     1381 ttcctggtct tcgcgttccc ggtgtactgg atgttctcga ccgcgttcaa gccggagcgg
     1441 gacatcctga cctacacgcc gcacccggtg ccctggccca tgacgctcca gcacttcagg
     1501 gacgcggtcg cccggcccca cttcctggac tacgtccgca acagcgtgct ggtcacctcg
     1561 agcgccgtcc tgctgtcgct ggccatcgcc acgctcgccg cgctcgcggt cggccggttc
     1621 gacttccgcg gccgcaaggc atacctgctg ctcgtgatgg tcgtgcagat ggcgccgttc
     1681 gaggcgctgc tgatcccgtt cttcctcatg ctgcgccagc tcgacctgct gaaccggctg
     1741 cccgcgctgg tcctcatcta cttcatcttc accatgccgt tcacgatctg gacgctgcgc
     1801 ggcttcgtca tggccgtgcc ccgcgagctc gaggaggccg ccatggtcga cggctgctcc
     1861 cgcctccagg cgttcatgcg ggtggtgttc ccgctgctgg cccccggcat ggtggccacc
     1921 tcgatcttcg cgttcgtcac cgcctggaac gagttcctgt acgcgttcgt gttcatgcgc
     1981 gaccccgaca agctcaccct gcccgtctgg ctcggcacct tcaggaccgc gttcggcgac
     2041 gactggggcg gcaccatggc cgcgtcggcg ctgttcaccc tgccggtgct cgcgttcttc
     2101 ctcgccatcc accgccggct ggcctccggg ctcacggcag gggcggtcaa gggctgatgg
     2161 gctcactcca gcggctcgcg gacgcctgcc tgctgcccgc cttcgagggc accaccgcgc
     2221 cggactgggt gcgccggcgg gtcgcggagg ggctcggcgg ggtcgtgctc tacgcccgca
     2281 acgtcgcgtc gcccgcccag gtcgagcgcc tgacggccgc cctgcgcgcc gagcgcccgg
     2341 cgctgctggt cgccaccgac gaggagggcg gcgacgtcac caggctcgag gccgccaccg
     2401 gcagctccta cccgggcaac ctggccctcg gcgccgccgg cgacccggcg ctcacccgcg
     2461 ccgtcggcgc agccctcggc gccgacctgg ccgcggccgg gatcagcctc gacttcgccc
     2521 cggtcgccga cgtcaactcc aacccggcga acccggtcat cggcgtccgg tcgttcggcg
     2581 ccgaccccgg ccgggtcgcg gcgcacgtcg tcgccatggt cgaggggctc cagggtgcgg
     2641 gggtggcggc gtgcgcgaag cacttcccgg ggcacggcga caccgcggtc gactcgcacc
     2701 tggagctgcc caccgccggt ggcgacgccg ccacgctggc cggcgcgctc gtgccgttcc
     2761 gcgccgcggt cgcggccggc gtcaggtgcg tcatgaccgc ccacgtccgt gtgccggcgt
     2821 acggtgagga cccggcgacc ctggaccgcc gggtcctcac cggcctgctg cgagaggagc
     2881 tcggcttcga cggcctcgtc gtcaccgacg ccctggagat gcgtgcgatc gcgggtaccg
     2941 tcggcttcgg cgagggcggc gtccgcgccc tcacggccgg ggccgacgcc ctgtgcatcg
     3001 ggggcgggca caccggcgag ggcacggtcg acctcctgcg cgacgccgtc gtcgacgcgg
     3061 tccgcgcggg gcggctcgcg gaggagcggc tcgcggaggc cgcggcccgc gtcgcccgcg
     3121 tgggcacctg ggcggcgggg ttccgggccg agcccggcgc cggacctggc gccgggccgc
     3181 cggccgggcg tccgggtccc cgccgcgggg acccggaggc cgggctcgac gcggcgaggc
     3241 gggccgtgcg cgccgagggc agcgtgcgcg tcggcaccgg cgccgtcgtc gtcgagctcc
     3301 gtcccggcgc gtcgatcgcc gtcgggaacg tgccctgggg cgtcggcgac ctcctggcca
     3361 gccgcgaccc caccgtcgag gtgatccgct gcgccgaggg cggccgcgtc gagggccacc
     3421 cgccggacgc cgcggcactc gccgcccgtg ccgccggccg cccgctcgtc ctcgtcgtcc
     3481 gcgacctgc
//
LOCUS       DASPYY010000214         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_179422, whole genome shotgun sequence.
ACCESSION   DASPYY010000214 DASPYY010000000
VERSION     DASPYY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179422"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VEP73_03295"
     CDS             <1..568
                     /locus_tag="VEP73_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25494.1"
                     /translation="EGSTLAVSHGCGRLSPMARLFVAVWPPPEVAIVLSALDRPAVPG
                     VRWSTPEQWMVRVRPLGHVDARVVPPLVDVLRDELDGAPAVECVLGLATRRLGGQWLG
                     APVAGLDDLAAVVFQATEELVPVTHPRPFQADVVLARGRVPKELSGRPVSASWTARSI
                     SLVADRSSPSAARYEDLAKIPLGARVSP"
     gene            complement(587..1036)
                     /locus_tag="VEP73_03300"
     CDS             complement(587..1036)
                     /locus_tag="VEP73_03300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYT25495.1"
                     /translation="MTDSSPGPMAGTANFINPAALHPPTGYTHVVEVTAGRPVYIAGQ
                     VALDPAGALVGPGDLRAQARQVFDNLRAALEAVGATFDQVVKLNYYLVDATQLPVVRE
                     VRDEYLNPRQLPASTAVEVRRLVRDDLLIEIEAVAVIASFQPVSLTA"
     gene            complement(1196..>1561)
                     /locus_tag="VEP73_03305"
     CDS             complement(1196..>1561)
                     /locus_tag="VEP73_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25496.1"
                     /translation="SLPWRVEVEAGALTADLDLRDLDVRAVGVDAGISRLAVRLGQPV
                     RQVPVDLQVSAGLVDLYVPRSADLEIRVDGLALNNFGAEALSRPKGATGWRTRSTAGP
                     GRYLVTVRISAGRVRVHRG"
BASE COUNT          188 a          552 c          596 g          225 t
ORIGIN      
        1 cgagggcagc accttggcag tctcgcacgg ctgtggcagg ttgtcgccga tggcgcgcct
       61 gttcgtcgcc gtgtggccac cgccggaggt tgccatcgtg ctctcggcgc tcgaccgccc
      121 ggcggtcccg ggcgtgcgct ggtcgacgcc cgagcagtgg atggtcaggg tgcgcccgct
      181 cggtcacgtg gacgcccggg tggtgccgcc gctcgtcgac gtgctccggg acgagctgga
      241 cggcgcgccc gccgtcgagt gcgtcctcgg cctggcgacc cgccggctcg gcgggcagtg
      301 gctgggcgca ccggtggccg ggctcgacga cctggccgcc gtggtgttcc aggcgaccga
      361 ggagctggtg ccggtcacgc acccgcggcc gttccaggcc gacgtggtgc tcgcccgggg
      421 ccgggtgccg aaggaactct ccggccggcc cgtttcggcg tcgtggacgg cacgctcgat
      481 ctccctcgtg gccgaccgtt cgtctcccag tgccgcccgg tacgaggacc ttgccaagat
      541 cccgctgggt gctcgagtaa gcccctgagc agtggccgca tcccactcat gctgtcaggc
      601 tgaccggctg gaacgacgcg atcaccgcga ccgcctcgat ctcgatgagg aggtcgtcgc
      661 gaaccagccg ccggacctcg acggccgtgc tcgcgggcag ctgccggggg ttgaggtact
      721 cgtcccggac ctcgcggacg accggcagct gggtggcgtc cacgaggtag tagttcagct
      781 tgaccacctg atcgaaggtg gcgccgacgg cctcgagtgc cgcccgcagg ttgtcgaaca
      841 cctgccgcgc ctgggcgcgg aggtccccgg gcccgacgag cgcaccggcg gggtcgaggg
      901 cgacctgccc ggcgatgtag acgggccggc ccgccgtcac ctcgacgacg tgcgtgtacc
      961 ccgtgggcgg gtggagcgcc gccgggttga tgaagttcgc ggtgcccgcc atgggcccgg
     1021 gcgacgaatc ggtcatacga ctgcttcccg tctcttgtga tgtccggcag cgacgcagtc
     1081 tacgacggag gtgcccggtc cctggttacg ggcagcctga tggcgaccgc tgacgcagga
     1141 ggaggtgccc ggggtggacc gggcggtcgg gacgaggtgc gggcggccgc ggggctcagc
     1201 cccggtgcac ccggacccgg ccggcggaga tccgcaccgt gaccaggtac cggccaggcc
     1261 cggcggtgct gcgggtccgc cagccggtgg cgcccttcgg cctgctgagt gcctccgccc
     1321 cgaagttgtt cagtgccagg ccgtccaccc ggatctcgag gtcggcgctc ctcggtacgt
     1381 agaggtcgac caggccggcc gagacctgca ggtcgaccgg cacctgcctg accggctggc
     1441 cgaggcggac cgcgagacgg gagatgccgg cgtcgacccc gacggcccgc acgtcgaggt
     1501 cgcggaggtc gaggtcggcg gtgagcgcgc ccgcctcgac ctcgacccgc cacggcaggc
     1561 t
//
LOCUS       DASPYY010000215         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1724550, whole genome shotgun sequence.
ACCESSION   DASPYY010000215 DASPYY010000000
VERSION     DASPYY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724550"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VEP73_03310"
     CDS             complement(<1..289)
                     /locus_tag="VEP73_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYT25497.1"
                     /translation="MADKGTVAVLGTGIMGGAMARRLCGAGFDVRVWNRTRERALPLA
                     DEGATVAGTPAEAADRAGYVLTMLTDGGAVEAVMFGPDGAAATAGDNAVWLQ"
     gene            complement(432..2285)
                     /locus_tag="VEP73_03315"
     CDS             complement(432..2285)
                     /locus_tag="VEP73_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25498.1"
                     /translation="MTGQLTFRAAATLAALAAVAFAIWMTFEIGGTATTTAVDDVAQG
                     AAALAAGVACLAAAHREPAGLGRAWSQALRRAWRLLAASAIVWGLGEGAWSYVELVQH
                     RPVPFPSTADVGYLAAVPLMLAAVLAFPTLPVRMAARLRTLLDALIIAASLLAVSWVT
                     VLGPMWNAAAPSASARTVALAYPMADILIATVAFSLLARPRGRGPVPMTLPAAALLCL
                     AVADSSFLYLTTSGSHRTGNLVDLGWLAGWLLFAVTALRPVEAQAVRGPAERVSLARL
                     CLPYALLAVAIFTYVMVQVTVHRLGPFLFGNGIALVVLVIARQLVTLVENRGLNRRLE
                     RMVQELSEREAQLETALDKEQTAAERLRAADAMKDTFLRAVSHDLRNPLTAILGVALT
                     LERTKLELPRDKGMELLGMLVDKAHKLDRLLADLLDLNRLEQGVLEPNRSPTDLDALV
                     RHLVGEIDHLEGRPVHVEMGQLVADIDAPKVERIIENLLINTTRHTPSATPVWIRARQ
                     RGRDLELVVEDAGPGVPPELAGTIFEPFRQGPTSGESSPGVGIGLSLVARFAQLHGGR
                     AWVGERRGGGAAFHVLLPNALLQHAPAVPLHEPEPATMEPAYPLLDRAQGA"
     gene            2673..4571
                     /locus_tag="VEP73_03320"
     CDS             2673..4571
                     /locus_tag="VEP73_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HYT25499.1"
                     /translation="MSLRATTPGSRQRARHVGSPSTCRSAVQAALDVLAWAVALAAAC
                     LARYDFQVASVVPAGLAAMTALAAVGQAGAGWFSGLYRGRRRFGSFDEVAVLAPATAF
                     VTALLFLADLAPGEARLVPLGATLGGGIGAFVLMGGVRYAWRLRLDRLLRPRGDGATR
                     LLVFGAGEGGVQVITSMLRNPAAPYLPVALLDDHPGKRHLRISGIPVVGTRHSIAQAA
                     RDHRATALLIAVPSAGADLVGELTDLANQAGLAVKVLPPVSELLDARVGVGDIRSLTE
                     ADLLGRHEIDTDVEAIAGYLTGRRVLVTGAGGSIGSELCRQIDRFAPAELVMVDRDES
                     ALHAVQLSIQGRALLDSPDVVLLDIRDRRRLGKVLAERRPEVVFHAAGLKHLPLLEQH
                     PGEAVRTNVLATLDLLELAARWGVDRFVNVSTDKAADPTSVLGWSKRIAERLTTQVAR
                     GSSGTFLSVRFGNVLGSRGSVLTTFRAQLAAGGPITVTTPDVTRYFMTVEEAVQLVIQ
                     AGAIGRGGEALVLDMGAPVRIASVAERLAAQADHPIEIVYTGLRPGEKLHEVLLGAGE
                     RDVRPIHPLISHVEVPPCDPELARRLDPTAPPGAVAGELRRLAAARAPRGEAHEPGRA
                     VTAPEGGR"
     gene            4573..5577
                     /locus_tag="VEP73_03325"
     CDS             4573..5577
                     /locus_tag="VEP73_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT25500.1"
                     /translation="MLNGALLTLALTLAISPLLLRALRRLELLDHPCWRSSHTQATLR
                     GGGLAPAFAATAMLSAVPVLDRTSRAGLAVVVIGFGAVGLLEDLRGVPPLPRFALQLL
                     AAALALPYLLTGLGGPAGWQVAFALGVAVWLVGYANAFNFMDGINGISAVQAIVAGLA
                     WLAVGHVQRADALAGGGVVIAAAAAGFLPFNFPRARCFLGDVGSYFFGSWIAALVVVG
                     LRSGVAPEAVLAPVILYLADTGTTLLRRVRRGEAWYLPHRDHVYQRLVRLGWSHARTT
                     VTVGVLTAGCALLGGTGSVAPPGARAVALGGLAVATATYLALPRIVGALGPAAPVADG
                     "
BASE COUNT          686 a         2159 c         2033 g          728 t
ORIGIN      
        1 tctggagcca gacggcgttg tcgccggctg tcgcggcggc gccgtccggg ccgaacatga
       61 ccgcctcgac ggcaccgccg tcggtcagca tcgtgaggac gtacccggcc cggtcggccg
      121 cctcggcggg cgtgccggcg acggtggcac cctcgtcggc gagcggcagc gcccgctccc
      181 tggtgcggtt ccagacccgc acgtcgaatc ctgccccgca gagccggcgc gccatcgcgc
      241 cgcccatgat cccggtcccc agcacggcga cggtgccctt gtcagccatg gtcggccctc
      301 gctctcactc gcggtcggcc ccgatcggtc ggctctccgt ggatcggctc tccatgaacc
      361 gaccctcggt gcatccattg tggacgaacg gctcggcagg ggcccccgcc ggcgcgagcc
      421 ggcggccggc cctacgcgcc ctgggcgcgg tcgagcaagg ggtaggcggg ctccatggtg
      481 gccggctccg gctcgtgcag cggcaccgcc ggcgcgtgct gcaggagcgc gttcggcagc
      541 agcacgtgga aggcggcccc gccgccccgc cgctcgccca cccaggcccg gccgccgtgg
      601 agctgggcga agcgggcgac gagcgacagc ccgatgccga cgccggggga gctctcgccg
      661 gaggtcgggc cctgccggaa cggctcgaag atcgtgcctg ccagctcggg cggcacgccg
      721 gggccggcgt cctcgacgac cagctcgagg tcgcgccctc gctggcgggc cctgatccac
      781 accggcgtcg ccgacggcgt gtggcgcgtc gtgttgatca gcaggttctc gatgatgcgc
      841 tcgaccttgg gcgcgtcgat gtcggcgacg agctgcccca tctcgacgtg gaccgggcgc
      901 ccctcgaggt ggtcgatctc cccgaccagg tggcggacga gggcgtcgag gtcggtcggc
      961 gagcggttgg gctcgaggac gccctgttcg agccggttca ggtcgagcag gtcggcgagc
     1021 agccggtcga gcttgtgcgc cttgtcgacc agcatcccga gcagctccat gcccttgtcg
     1081 cggggcagct ccagcttcgt ccgctccagg gtgagggcca ccccgaggat cgcggtcaag
     1141 gggttgcgca ggtcgtggga gacggcccgc aggaaggtgt ccttcatcgc gtccgcggcg
     1201 cgcaggcgct cggccgcggt ctgctccttg tcgagcgcgg tctcgagctg ggcctcccgc
     1261 tccgacagct cctggaccat ccgctcgagg cgccggttga gcccgcggtt ctccaccagg
     1321 gtgacgagct ggcgggcgat cacgaggacc accagggcga tcccgttgcc gaacaggaag
     1381 ggcccgaggc ggtggaccgt gacctgcacc atgacgtagg tgaagatggc gaccgcgagc
     1441 agcgcgtacg gcaggcagag gcgggcgagc gacacccgct cggccggccc cctgaccgcc
     1501 tgggcctcca ccgggcgcag ggccgtcacc gcgaacagca gccagccggc cagccagccg
     1561 aggtcgacga ggttgccggt cctgtggctg cccgaggtgg tgaggtacag gaagctcgag
     1621 tcggccaccg cgaggcagag cagcgcggcg gcgggcagcg tcatcggcac cgggccccgc
     1681 ccgcgcggcc gggccaggag cgagaacgcg accgtggcga tgaggatgtc ggccatcggg
     1741 taggcgagcg ccacggtacg ggcgctcgcc gacggggcgg cggcgttcca catcgggccg
     1801 agcacggtca cccagctcac cgcgagcagg gacgcggcga tgatgagcgc gtcgagcagg
     1861 gtccgcagcc gtgccgccat gcgcacgggc agggtcggga acgcgagcac ggcggcgagc
     1921 atcaggggca cggcggcgag gtagccgacg tcggccgttg acgggaacgg caccggccgg
     1981 tgctggacga gctcgacgta ggaccaggcc ccctcgccga gcccccagac gatcgccgac
     2041 gccgcgagca ggcgccaggc ccggcgcagc gcctggctcc acgccctgcc gagcccggcc
     2101 ggctcgcggt gggcggcggc caggcaggca accccggccg ccagcgcggc cgcgccctgg
     2161 gcgacgtcgt cgaccgccgt ggtcgtcgcg gtcccgccga tctcgaacgt catccagatc
     2221 gcgaaggcga cggcggcgag cgccgcgagg gtcgcggcgg ctcggaaggt cagctgtccg
     2281 gtcacggctt ccgggtcgct ccacggggtc ggtcggacgg tacctgccca tcttgcgcgc
     2341 ccggaggctt gcggactagg gcatcaggcg caaaaaaacc aaaacagcac aggatgaccg
     2401 cgtgagaagc ggtcggggac acaccggcag cacggctcat gtcggcctcg acggcggcgc
     2461 gccgggggcg cacctcgaac atggcaaacc agtacaacgg gacaccctac gatacaccag
     2521 cacacactgg cattcttgcc gagcggggcc ccttctggaa ccccggggta aaggtcggcc
     2581 ctccgtggtt cgacatggtg gacgcggctg gagccgccgg atgcggttcc ggacctgtca
     2641 aacctcccga acccgacaag gcagaggggc tagtgagcct gcgcgcgacc acaccaggca
     2701 gcaggcagcg ggcacggcac gtcgggtcgc cctcgacctg ccgttcggcc gtgcaggcgg
     2761 cgctcgacgt cctcgcgtgg gcggtcgcgc tcgccgccgc ctgcctcgca cgctacgact
     2821 tccaggtggc gtccgtcgtc ccggcgggcc tggccgccat gaccgccctc gccgcggtcg
     2881 ggcaggccgg ggccggctgg ttcagcggcc tgtaccgggg gcggcgccgc ttcgggagct
     2941 tcgacgaggt ggcggtgctc gcgccggcca ccgccttcgt caccgcgctg ctgttcctcg
     3001 ccgacctggc tcccggggag gcccggctgg tgcccctcgg ggccacgctc ggcggaggga
     3061 tcggcgcgtt cgtgctcatg ggcggcgtcc gctacgcctg gcgcctgcgg ctcgaccgcc
     3121 tgctccgccc gcggggcgac ggcgccaccc gcctgctcgt cttcggtgcc ggcgagggag
     3181 gcgtccaggt gatcacctcg atgctccgca accccgcggc gccctacctg ccggtcgcgc
     3241 tcctcgacga ccacccggga aagcgccacc tgcgcatcag cgggatcccg gtcgtgggca
     3301 cccgccactc gatcgcccag gcggcacggg accatcgggc caccgccctc ctcatcgccg
     3361 tcccgagcgc cggcgccgac ctcgtcggcg agctgaccga cctcgccaac caggccgggc
     3421 tcgccgtcaa ggtgctcccg cccgtgagcg agctgctcga cgcccgggtc ggcgtcggcg
     3481 acatccgcag cctcaccgag gccgacctgc tcggccgcca cgagatcgac acggacgtcg
     3541 aggcgatcgc cggctacctg accggccggc gggtgctcgt gaccggggcc ggcgggtcga
     3601 tcggctcgga gctgtgccgg cagatcgacc gcttcgcccc ggccgagctg gtgatggtcg
     3661 accgggacga gtcggcgctg cacgcggtgc agctctcgat ccagggacgg gcgctgctcg
     3721 actcgcccga cgtggtgctc ctcgacatcc gcgaccggcg ccgcctcggg aaggtgctcg
     3781 ccgagcgccg gcccgaggtg gtgttccacg ccgcggggct caagcacctg ccgctgctcg
     3841 agcagcatcc cggcgaggcg gtcaggacca acgtgctggc aacgctcgac ctgctcgagc
     3901 tcgcggcgcg gtggggcgtc gaccgcttcg tcaacgtctc caccgacaag gcggccgacc
     3961 cgaccagcgt gctcggctgg tccaagcgca tcgccgagcg cctcaccacc caggtggccc
     4021 gcggctcgtc agggaccttc ctgagcgtcc gcttcggcaa cgtgctcggc agccgggggt
     4081 cggtgctcac caccttccgc gcccagctgg ccgcgggcgg cccgatcacg gtcaccaccc
     4141 cggacgtcac ccgctacttc atgaccgtcg aagaggccgt ccagctggtc atccaggcgg
     4201 gggccatcgg caggggcggg gaggcgctcg tcctcgacat gggggcgccg gtcaggatcg
     4261 catcggtcgc cgagcggctc gccgcccagg ccgaccaccc gatcgagatc gtctacacgg
     4321 ggctgcggcc gggcgagaag ctccacgagg tcctgctcgg cgccggcgaa cgggacgtcc
     4381 gccccatcca cccgctgatc tcccacgtcg aggtcccgcc gtgcgacccg gagctggcgc
     4441 gccggctcga cccgaccgcc ccacccggcg ccgtcgccgg cgagctcagg cggctggcag
     4501 cggctcgcgc gccacggggc gaggcgcacg agcccggacg ggccgtcacc gccccagagg
     4561 gaggtcgctg agatgctcaa cggcgccctg ctcaccctcg cgctcaccct cgccatctcc
     4621 ccgctcctgc tccgcgccct gcggcggctc gagctcctgg accacccctg ctggcgctcc
     4681 tcgcacacgc aggcgaccct gcgcggcggc ggcctcgccc ccgccttcgc cgccaccgcg
     4741 atgctctccg cggtgccggt cctcgaccgg acgtcccgcg ccggcctcgc ggtcgtcgtc
     4801 atcggcttcg gcgcggtcgg gctcctcgag gacctgcgcg gcgtcccgcc cctgccgcgg
     4861 ttcgcgctcc agctcctcgc cgcggcgctg gcgctgccct acctgctcac cggcctcggc
     4921 ggaccggcgg gctggcaggt cgcgttcgcc ctcggggtgg cggtctggct cgtcggctac
     4981 gcgaacgcct tcaacttcat ggacggcatc aacgggatct ccgccgtcca ggccatcgtc
     5041 gccggcctcg cctggctcgc ggtcggccac gtccagcggg ccgacgccct ggccggcggc
     5101 ggcgtggtga tcgccgccgc ggcagccggc ttcctgccct tcaacttccc gcgggcgcgc
     5161 tgcttcctcg gcgacgtcgg cagctacttc ttcgggtcgt ggatcgcggc gctcgtcgtc
     5221 gtcgggctgc gctccggcgt cgcgcccgag gcggtcctcg cgccggtcat cctctacctg
     5281 gccgacaccg gcaccaccct gctccgccgg gtgcggcgcg gcgaggcctg gtacctgccg
     5341 caccgggacc acgtctacca gcggctcgtc cggctcggct ggtcgcacgc ccgcacgacc
     5401 gtgaccgtcg gcgtcctcac cgccgggtgc gccctgctcg gcggcaccgg ctcggtcgcc
     5461 ccgcccggcg cccgggccgt cgcgctcggc ggcctcgccg tcgcgaccgc cacctacctg
     5521 gccctcccgc gcatcgtcgg cgccctggga cccgccgccc cggtggccga tggctgagcc
     5581 ccgcaccgcc gcgcggcggc tcagga
//
LOCUS       DASPYY010000216         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_319224, whole genome shotgun sequence.
ACCESSION   DASPYY010000216 DASPYY010000000
VERSION     DASPYY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319224"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..389
                     /gene="rpsI"
                     /locus_tag="VEP73_03330"
     CDS             6..389
                     /gene="rpsI"
                     /locus_tag="VEP73_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYT25501.1"
                     /translation="MLARGTGRRKEAVVRVRLLEGTGAFKLNGRPLEDYFPNPAHRRL
                     VTEPLRVTNTLERYDIIASLSGGGVSGQAGALRHGIARALVDLDVELRGELKKRGFLT
                     RDARETERKKYGLKKARKAPQYSKR"
     gene            complement(483..1172)
                     /locus_tag="VEP73_03335"
     CDS             complement(483..1172)
                     /locus_tag="VEP73_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25502.1"
                     /translation="MSEPSPDGIIMDHPPRRAPLPDDAWGSPLGSSPTAPIPEWDAEP
                     PPDGPPRRAAALWVVLGAAALGAALAGVFAVAKLVGTAPGAGGPLRTPAAVAGLRRVD
                     SPALRPLLGQQERQLRDRGIRNYVVAAYGTGAQAQLVVVAVREAGGRTRGDIVKGFDA
                     ELARSVPGAGPDQVFEHGKVEYRCTASRLGASFSVCRWNDGDVVGFGFSPSSGPDRLS
                     RLAAAARAAMV"
     gene            1318..>2030
                     /locus_tag="VEP73_03340"
     CDS             1318..>2030
                     /locus_tag="VEP73_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYT25503.1"
                     /translation="MGKLFGTDGVRGVANAELTPELALSLGRAVVGVLRATEPDRDRR
                     PLVVVGRDPRASGALLESALVAGVCSAGGDALLAGVLPTPAVAFLTRHYGAQAGAVIS
                     ASHNPMPDNGIKFFGPEGFKLQDPVEDRIEAALGLADQDAPRPVAGAVGRVRQATDAL
                     EAYLDHLLEDVGDLAGLPVVVDCANGAAAAVAPEAYRRAGARVVAVAAEPDGENINAG
                     VGSTHPEHVQAEVRRARAAV"
BASE COUNT          270 a          764 c          766 g          230 t
ORIGIN      
        1 ccgcactgct cgcccggggc accggccgcc gcaaggaggc cgttgtccgg gtgcgcctgc
       61 tcgaggggac cggggcgttc aagctgaacg gccgcccgct cgaggactac ttccccaacc
      121 cggcgcaccg ccggctcgtc accgagcccc tgcgcgtcac caacacgctc gagcgctacg
      181 acatcatcgc cagcctgagc ggcggcgggg tctcgggcca ggccggggca ctgcgccacg
      241 gcatcgcccg cgccctggtc gacctcgacg tcgagctgcg cggcgagctc aagaagcggg
      301 ggtttctgac ccgcgacgcc cgggagaccg agcgcaagaa gtacgggctc aagaaggccc
      361 gcaaggcgcc ccagtacagc aagcgctgac cccgggcgct tggagcgctg gtaggcaagc
      421 gctggccagc gcggcgagca gccaggacgc ccgccgggtc cggacccggc gggcgtcggc
      481 cgtcacacca tcgccgcccg cgcggcggcg gccagcctgc tcaggcggtc cggccccgac
      541 gacgggctga agccgaagcc cacgacgtcg ccgtcgttcc accggcagac ggagaagctc
      601 gcgccgagcc gggacgcggt gcagcggtac tcgaccttgc cgtgctcgaa cacctggtcg
      661 gggccggcgc caggcaccga ccgcgcgagc tcggcgtcga accccttgac gatgtcgccg
      721 cgggtccggc cgccggcctc ccggaccgca acgaccacga gctgcgcctg tgccccggtg
      781 ccgtacgcgg ccaccacgta gttcctgatg cccctgtcgc ggagctgccg ctcctgctgg
      841 ccgagcaggg gccgcaacgc ggggctgtcg acccgccgca ggccggccac tgccgccggc
      901 gtccgcagcg gcccgcccgc ccccggggcg gtgccgacga gcttggcgac ggcgaagacc
      961 ccggcgagcg cggcgccgag cgcggcggcg ccgagcacga cccacagcgc cgcggcgcgc
     1021 cgcggcgggc cgtccggtgg cggctccgcg tcccactccg ggatcggcgc cgtcggggag
     1081 gagcccagcg gggagcccca ggcgtcgtcg gggagcggtg cacggcgagg aggatggtcc
     1141 atgatgatcc cgtctggtga gggctcggac atgcccgggc gtcgactggc tgcgaatcga
     1201 cagcagcttt agcagctttg cccggcttcc gcacctcacg cgaaccagtc gagctgggca
     1261 tcggcgcatg caacaatgac cagctcgcgc gagacgacca ggacgagggg tgcgctgttg
     1321 ggaaagctgt tcggaaccga cggggtgcgt ggggtggcca acgccgagct cacccccgag
     1381 ctggccctgt cgctcgggcg ggcggtggtc ggcgtgctgc gggccaccga gcccgaccgt
     1441 gaccggcgcc cgctggtcgt ggtggggcgc gacccgcgcg cctcgggtgc cctgctggag
     1501 agcgccctgg tggcaggggt gtgcagcgcg ggcggcgacg cgctcctggc cggcgtgctc
     1561 ccgacacccg cggtggcctt cctcacgagg cactacggtg cccaggccgg tgccgtgatc
     1621 tcggcctccc acaaccccat gccggacaac ggcatcaagt tcttcgggcc cgaggggttc
     1681 aagctgcagg acccggtgga ggaccggatc gaggcggcgc tgggcctcgc cgaccaggat
     1741 gcgccccggc cggtcgccgg cgcggtgggc cgtgtccgcc aggccaccga cgcgctcgag
     1801 gcatacctcg accacctgct ggaggacgtc ggcgacctcg ccggcctgcc ggtcgtggtc
     1861 gactgcgcca acggcgcggc cgccgcggtc gccccggagg cctaccggcg tgccggcgcc
     1921 cgggtcgtcg cggtcgccgc cgagcccgac ggggagaaca tcaacgccgg ggtcggctcg
     1981 acccaccccg agcatgtcca ggccgaggtc aggcgtgccc gggcggcggt
//
LOCUS       DASPYY010000217         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1724667, whole genome shotgun sequence.
ACCESSION   DASPYY010000217 DASPYY010000000
VERSION     DASPYY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724667"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VEP73_03345"
     CDS             <1..824
                     /locus_tag="VEP73_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYT25504.1"
                     /translation="AIATSAPHARSANLHPFAPHGWLAVGGAATLLVWSFAGWETVTH
                     LAAEFADPARDLRRATAAALALIGALYLGLATDSVLVLGPQLERVDAPLAVLLASGLG
                     GSVGTIAAVVAVVVTLGTMNAYVASAARLGAALGRDGALPRWLAKGAQAGEVPRRSLA
                     AVAALTGLVLSGLLVTGADAHPLVLATTSCLAAIYAVGCAAGIRLLPRGSFGRRAAAA
                     ATALVTGLLLLAGPFLAWPLVLAAGALVTRRRLLLGRIVPAGGLVGPGSRLRGMR"
     gene            1010..1408
                     /locus_tag="VEP73_03350"
     CDS             1010..1408
                     /locus_tag="VEP73_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25505.1"
                     /translation="MTTPQAPRVLLVDDEANMRRSLRYLLEVEDITVVGECGDGVEAV
                     ALAQEVRPDVVLMDLRMPGLNGLEATRQIKGVLPGTQVVIFTAYDDSGLGRQAEQAGA
                     YRCLVKGCSGDELVDLVFQAYKAGDRPSGD"
     gene            1559..2656
                     /locus_tag="VEP73_03355"
     CDS             1559..2656
                     /locus_tag="VEP73_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYT25506.1"
                     /translation="MGVFEKMAESGHEQVVFCYDRQTGMKAIIAIHDTALGPARGGTR
                     YRTYESEDDALDDVLRLSQGMTYKFAFTDMPRGGGKAVLLKEDGGADKRVLLRVFGRF
                     VQLLAGRFGTGPDLGTSAADMIDVARETDYVWAIDERFGGTGDSTPLTAQGVLAGIGA
                     ALDEVDGSPDVRDRRIVVQGLGGVGGHLAGLLVAGGATVIGADIDPARAKELAERMGI
                     ELVDPDAVYEVPCDVFSPNAVGGVLDERTIPRLACRVVAGGANNQLATAADGRRLMER
                     GILYAPDYVINSAAPYAVIGAGELGYSEARLAVSVQGVAASLQLVFRRARAEGRPTVE
                     VADTIAEERVAAAAHIRAMTPGTGSLARQRP"
     gene            2839..>3193
                     /locus_tag="VEP73_03360"
     CDS             2839..>3193
                     /locus_tag="VEP73_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25507.1"
                     /translation="MDDYTGRITIIASSTMVHHRVKCMVCKQPVDRRVVFLTNDARVL
                     HTGCVPNRVKVPNTVLLQAIRRLERMVERARANPFYDALDPYEPAGSLDPGVLDAYEP
                     IGALTVHDPDGDRDPD"
BASE COUNT          405 a         1142 c         1193 g          453 t
ORIGIN      
        1 tcgcgatcgc cacctcggct ccgcatgccc gcagcgccaa cctgcacccg ttcgcgcccc
       61 acggctggct cgcggtcggc ggggcggcga cgctgctggt ctggtcgttc gccggctggg
      121 agacggtcac ccacctcgcc gcggagttcg ccgaccccgc ccgcgacctc cgccgggcaa
      181 ccgccgcggc acttgctctg atcggcgccc tgtatctcgg cctcgccacg gacagcgtgc
      241 tggtgctcgg cccgcagctc gagcgcgtcg acgcgcccct cgccgtgctg ctcgccagcg
      301 gcctcggcgg ctcggtcggc acgatcgcgg cggtggtcgc tgtggtggtc acgctcggca
      361 cgatgaacgc gtacgtggcc agcgccgcca ggctcggcgc ggccctcggc cgggacgggg
      421 cgctgccgcg ctggctcgcg aagggcgcgc aggccgggga ggtgccccgg cgcagccttg
      481 ccgccgttgc cgcgctgacg ggcctggtgc tctcggggct gctcgtcacc ggggcggacg
      541 cgcacccgct ggtgctcgcg accacctcgt gcctggccgc gatctacgcg gtcggctgcg
      601 ccgccgggat ccgcctcctc ccccggggca gcttcggccg ccgcgcggcg gccgccgcga
      661 cggccctggt caccgggctg ctgctgctcg ccggcccgtt cctcgcatgg ccgttggtct
      721 tggccgcggg cgcgctcgtc acccgccgcc gcctcctgct cggccggatc gtccctgccg
      781 gcggcctggt gggcccggga agccggcttc gaggaatgcg ataggctcga ggccggctgc
      841 gcttccgagg gtccgcgagg ggatggtgcc cgtcgccgct ccctgcaccg aggcggcagg
      901 cgggtcagga cgtctcgccg gatcttgact gtcttggccg ggaagtagag attgacggct
      961 tcttgggaat gctccgcccg tcgagggggt cagtggtgcg ggacgacaga tgacgacacc
     1021 ccaggcaccg cgcgtgctgc tggtggacga cgaggccaac atgcgccggt cactgcgcta
     1081 tctgctcgag gtcgaggaca tcaccgtcgt cggggaatgc ggcgacggcg tcgaggcggt
     1141 ggcgctcgcc caggaggtcc gacccgacgt ggtcctgatg gacctgcgga tgccgggcct
     1201 caacggcctg gaggccaccc gccagatcaa gggtgtcctg cccggcacgc aggtggtcat
     1261 cttcaccgcc tacgacgact cggggctcgg gcgccaggcc gagcaggcgg gcgcctacag
     1321 gtgcctggtg aagggctgca gcggcgatga gctggtcgac ctcgtgttcc aggcgtacaa
     1381 ggcgggcgac cggccctccg gggactgaac cgcgccgccc gcgaccaggc tcgtccgccc
     1441 agggcttcgg gtacccttga ggtgcgacgg tgctcgccgc cgcgcccacc cggcaacggc
     1501 gctgggagcc tcgttgacgc cctggccgtg cgggcatgaa ccgtccaggg aggcatgtat
     1561 gggcgtcttc gagaagatgg ccgagtccgg ccacgagcag gtcgtgttct gctacgaccg
     1621 ccagacgggg atgaaggcga tcatcgccat ccacgacacc gcgctcgggc ccgctcgcgg
     1681 ggggacccgc taccggacct acgagtccga ggacgacgcc ctcgacgacg tgctgcgcct
     1741 gtcccagggc atgacctaca agttcgcctt caccgacatg ccccggggcg gcggcaaggc
     1801 ggtgctcctc aaggaggacg gcggagccga caagcgggtg ctgctccggg tgttcggccg
     1861 gttcgtgcag ctcctcgccg gtcgcttcgg gaccggtccc gacctcggca ccagcgcggc
     1921 cgacatgatc gacgtggcca gggagaccga ctacgtgtgg gcgatcgacg aacggttcgg
     1981 cggcacgggg gactccacgc cgctgaccgc ccagggcgtg ctggccggga tcggcgccgc
     2041 cctggacgag gtcgacggct cccccgacgt ccgcgaccgg cggatcgtcg tccaggggct
     2101 gggcggggtc ggcgggcatc tcgccgggct gctggttgcg ggcggcgcca ccgtgatcgg
     2161 ggccgacatc gacccggcgc gggccaagga gctggccgag cggatgggga tcgagctggt
     2221 cgacccggac gcggtgtatg aggtgccctg cgacgtgttc agccccaacg cggtcggcgg
     2281 ggtgctcgac gaacggacga tcccccggct ggcctgtcgt gtcgtcgccg gaggggcgaa
     2341 caaccagctc gccaccgcgg cggacggccg ccgcctgatg gagcgcggga tcctctacgc
     2401 gcccgactac gtgatcaact cggcggcgcc gtacgcggtg atcggcgccg gcgagctcgg
     2461 gtactcggag gcccggctgg ccgtgtcggt gcagggggtc gcggcctcgc tgcagctcgt
     2521 cttcaggcgc gcccgggccg aggggcggcc gaccgtggag gtggcggaca cgatcgccga
     2581 ggagcgggtc gcggccgccg cgcacatccg cgccatgacc ccgggcaccg ggagcctcgc
     2641 ccgccagcgc ccttgagccg ggacggcgct ccggccccgg gcatatcttg gatattgctg
     2701 tccggggcgt ccacagagcc gagagatggt ggcaagcttc gcagtacaga tggctcgggc
     2761 cgcgagggcg tgggtgaccg agcggggggc gcgtaggcgg aaggtgctgc ttccggctcc
     2821 ctgggaaggc gttgccgcat ggacgactac acaggccgca tcaccatcat cgcgtcgtcg
     2881 acgatggtcc atcaccgcgt caagtgcatg gtctgcaagc agccggtcga ccgtcgtgtg
     2941 gtgttcctga ccaacgacgc gcgggtcctg cacaccggtt gcgtgcccaa ccgggtgaag
     3001 gtcccgaaca ccgtgcttct gcaggccatc cggcggctcg agcggatggt ggagcgggcc
     3061 agggccaacc ccttctacga cgccctcgac ccctacgagc ccgccgggtc gctcgaccca
     3121 ggcgtcctgg acgcctacga gccgatcggc gccttgaccg tccacgaccc ggacggcgac
     3181 cgcgaccccg acc
//
LOCUS       DASPYY010000218         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1866141, whole genome shotgun sequence.
ACCESSION   DASPYY010000218 DASPYY010000000
VERSION     DASPYY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866141"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..507)
                     /gene="rplL"
                     /locus_tag="VEP73_03365"
     CDS             complement(115..507)
                     /gene="rplL"
                     /locus_tag="VEP73_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYT25508.1"
                     /translation="MPKISSDDLLDAFKEMTLLELSEFVKQFEDTFGVSAAAPVAVAV
                     GPTTDGGAAQAAPEEEQIEFDVVLNAAGDKKIQVIKEVRSLTSLGLKEAKDLVDGAPK
                     PLLEHVNKEAADKARAALEGAGATVTVK"
     gene            complement(522..1103)
                     /gene="rplJ"
                     /locus_tag="VEP73_03370"
     CDS             complement(522..1103)
                     /gene="rplJ"
                     /locus_tag="VEP73_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYT25509.1"
                     /translation="MPNASNVAEVQAIKERLEGSTAALLTEYRGLKVSELGQLRASLR
                     GSDTDYKVVKNTLTSIAVRQVGYEELVGLLEGPTAVAFVRGDVVQAAKDLAEFARTHP
                     SLVVKGGVLEGKVLDADGVRRLATLESREVMLARMAGLLQAPLQQAVNLLAAPLRQVA
                     TMTAALRDKLEEEGGTPDAAAPTDTAADPGAPQ"
     gene            complement(1341..>1876)
                     /locus_tag="VEP73_03375"
     CDS             complement(1341..>1876)
                     /locus_tag="VEP73_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25510.1"
                     /translation="PGGPAAAFLVVGGLVLDFGVRRLLRRQGLAAPPFGSAFAWLAEG
                     DREGAGTWFARATVLPGRGVLVRFGRPDAPRWDELAVDPEAAPALPAGREPALLELTE
                     ALGWSVVRLVEDALPAFDPVRRRIPGADDEGAFLAWSWTLAVDAALDGLAARGLLAPP
                     IDGAVAVRVADPALAGM"
BASE COUNT          237 a          692 c          704 g          243 t
ORIGIN      
        1 cgggcggggc gacgggcggg aaccggtaac cgctgcctgc gggctcaacc ggcacccgga
       61 accgatgtgc ccggccggct gacgccggcc gggcacatcg ccgttaccgg accgttactt
      121 gactgtgacg gtcgcgccag cgccctcgag cgcggccctg gccttgtccg ccgcctcctt
      181 gttgacgtgc tccagcagcg gcttgggcgc gccgtccacc aggtccttgg cctccttcag
      241 gccgaggctg gtcagggagc ggacttcctt gatgacctgg atcttcttgt cgccggcggc
      301 gttcaggacg acgtcgaact cgatctgctc ctcctcgggg gcggcctggg ccgcgccgcc
      361 gtcggtcgtc gggccgacgg cgaccgcgac cggcgcggcc gccgagacgc cgaaggtgtc
      421 ctcgaactgc ttcacgaact ccgacagctc gaggagggtc atctccttga aggcgtccag
      481 gaggtcgtcg ctggagatct tgggcatggt ctgcttgctc cttactgtgg cgcgccgggg
      541 tcggcggcgg tgtcggtcgg ggccgcggcg tcgggcgtgc cgccctcctc ctcgagcttg
      601 tcgcgcaggg cggcggtcat ggtcgccacc tgccgcaacg gggcggccag caggttgacg
      661 gcctgctgga gcggggcctg cagcagcccg gccatccggg ccagcatgac ctcgcgggac
      721 tcgagcgtgg ccagccggcg cacgccgtcg gcgtcgagca ccttgccctc gagcacgccc
      781 cccttgacca ccagggacgg gtgggtgcgg gcgaactcgg caaggtcctt ggcggcctgg
      841 accacgtcgc cgcggacgaa ggccaccgcg gtcgggccct cgagcagccc gacgagctcc
      901 tcgtagccca cctggcggac ggcgatgctg gtcagcgtgt tcttgacgac cttgtagtcg
      961 gtgtcggacc cgcgcagcga cgcacgcagc tggccgagtt cggagacctt gaggccccgg
     1021 tactcggtca gcagggcggc cgttgagccc tccagacgtt ccttgatggc ctggacctca
     1081 gcgacgttgc tcgcattcgg cacggcccca cctccctgac ctctcaagcg cacacaggaa
     1141 acggacggtc ccggaccgcc cgttggcgcg cgcacgactc ggttgcggag gtgctcgcct
     1201 gcgtcggcgc ccgccgtgcg gaactcggac gggccttcgt gtcgcgctgc gacaaccgag
     1261 cggtcttcgg ctcgggcgag ggtagcgggg ggctccacgc tcgtcaaccc ggggccacat
     1321 gcccggggcc tgccccgggg tcacatgccg gcgagcgcgg gatccgccac ccggaccgcg
     1381 acggcgccgt cgatcggcgg ggcgagcagg ccgcgggcgg cgaggccgtc gagcgccgcg
     1441 tccacggcca gggtccacga ccaggccagg aaggcgccct cgtcgtcggc accggggatg
     1501 cggcggcgga cggggtcgaa ggcgggcagc gcgtcctcga cgagccggac caccgaccag
     1561 ccgagcgcct cggtcagctc caggagcgcc ggctcgcggc cggccggcag cgcgggcgcc
     1621 gcctcgggat cgaccgcgag ctcgtcccag cggggagcgt ccgggcgccc gaagcgcacc
     1681 agcacccccc ggcccgggag gacggtggcg cgggcgaacc acgtgcccgc cccttcgcgg
     1741 tcgccctcgg ccagccaggc gaaggcgctg ccgaacggcg gggcggcgag gccctgccgg
     1801 cgcagcaggc gccgcacccc gaagtcgagg accagcccgc ccacgacgag gaacgcggcc
     1861 gccgggccgc cggggc
//
LOCUS       DASPYY010000219         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_461316, whole genome shotgun sequence.
ACCESSION   DASPYY010000219 DASPYY010000000
VERSION     DASPYY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461316"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VEP73_03380"
     CDS             complement(<1..399)
                     /locus_tag="VEP73_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25511.1"
                     /translation="MLAPVDNDAVVLAVLADRFRLPMERLVPGEIAVAEVPHPSLPVR
                     AFTRDGGAVVAVAPEHARLLAAHLRGPATIRPSLEAVAAELPGHMFSGVARTGVAIPP
                     PAVPVDVLDAADPRLPDWVHGYFTGAAWVVL"
     gene            521..1306
                     /locus_tag="VEP73_03385"
     CDS             521..1306
                     /locus_tag="VEP73_03385"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HYT25512.1"
                     /translation="MAALPPARAGRGTPPGAVSGAGYMARGIRVFLATPGVRLLGIVP
                     VLIAAVLVLAALALLVTRLAAVAAWLTPFADSWPQTTRTSLRDAVGVAVLLGALLLAV
                     LAFATLANVIGQPFYERISDRVERRLGAPPPGAGGPWWRTLPRATAESAVLLALVVGA
                     NLPLVLLELVPVLGQSLVPVVQAVVSGFLLSIELLAIPLERRGLHLRARLRFAWRHRA
                     PVTGFGVAAFLLFLVPLANVVAMPGAVVGGTLLVRRLTGAPDR"
     gene            1420..2229
                     /gene="dapB"
                     /locus_tag="VEP73_03390"
     CDS             1420..2229
                     /gene="dapB"
                     /locus_tag="VEP73_03390"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYT25513.1"
                     /translation="MRVGVLGATGRMGQAVCRAVLAAEDLELVAAVARKTGVGHELRT
                     VIPEAPPDLLVGQYLSDLISAEVDVVVDFSVPEATVIAARTLLPEGIHLVSGTTGLPE
                     GATEELPGLARADEAGNVVWAANFALGAVLAIHFATVAARFYPAEIVELHHAAKADAP
                     SGTAIRTAHAVAAERAATDGAGPGLAGPGREVVAGARGADVDGVRVHSVRLPGLVAHQ
                     EVVFGGQGELLTVRHDALDRAAYMPGVLLAVRQVPLRPGLTVGLEPLLGLA"
     gene            2226..>2882
                     /locus_tag="VEP73_03395"
     CDS             2226..>2882
                     /locus_tag="VEP73_03395"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYT25514.1"
                     /translation="MTAGAATRPAYWVEEFDDAERARLEPWFTNTDGPVFALRNLPET
                     VKGALFARYSRYQGTLRRLFLDEFADSLPDAPAVPEGGEGKRAAELYERIFLGYGDDS
                     VAQLGGAHVACEWVSNVLTKVLQRPRLAAYLEQSTRYIAYDEPMDSSGRYRYYRDPDL
                     GPEYGAAMDDLFGIYSAALPRVIEWASSAFPLGEGEAPGAHARAIKAKALDLLRGLLP
                     A"
BASE COUNT          297 a         1106 c         1092 g          387 t
ORIGIN      
        1 gagcacgacc cacgccgcgc cggtgaagta cccgtggacc cagtcgggca gccgggggtc
       61 ggcggcgtcc aggacgtcga ccgggaccgc gggcgggggg atggccaccc cggtccttgc
      121 cacgcccgag aacatgtgcc cgggcagctc ggccgcgacc gcctcgagcg acggccggat
      181 cgtggccggg ccccggaggt gggcggcgag cagccgcgcg tgctcgggcg cgaccgcgac
      241 caccgccccg ccgtcgcggg tgaacgcgcg gacggggagg ctcgggtgcg ggacctcggc
      301 caccgcgatc tcgccgggga ccaggcgctc catgggcagc cggaaccggt ccgcgagcac
      361 ggcgaggacg acggcgtcgt tgtcgacggg tgcgagcata gggtcagcgt aacggtcccg
      421 gggggtcccg gacaggtcta tgtcggcggg gggtggggac aggtggtcgc tccgcgccgc
      481 cgccccgcgg gccgccgccc gctttgctac gatccgcgcc atggccgccc tgcctcccgc
      541 tcgcgcgggc cggggaacgc cgccgggggc ggtctccggt gccggctaca tggcccgggg
      601 catccgtgtg ttcctcgcga cacccggggt ccggctcctc gggatcgtcc ccgtcctgat
      661 cgccgccgtg ctcgtgctgg cggcgctcgc cctgctcgtg acgcgcctcg ccgccgtcgc
      721 cgcctggctc accccgttcg ccgactcctg gccgcagacg acccggacct ccctgcggga
      781 cgccgtcggc gtggccgtgc tgctcggcgc cctgctgctc gccgtgctcg ccttcgcgac
      841 cctcgccaac gtgatcgggc agcccttcta cgagcggatc tcggaccggg tcgagcgccg
      901 cctcggcgcc ccgccgcccg gcgccggcgg cccgtggtgg cggaccctgc cccgggcgac
      961 cgccgagtcg gcggtcctcc tcgcgctcgt ggtcggcgcc aacctcccgc tcgtcctgct
     1021 cgagctcgtc cccgtgctcg ggcagagcct cgtgcccgtg gtccaggcgg tcgtctccgg
     1081 cttcctgctg tcgatcgagc tgctcgcgat cccgctcgag cggcgggggc tgcacctgcg
     1141 ggcgcgcctc cggttcgcct ggcggcaccg ggcccccgtg accgggttcg gggtggcggc
     1201 gttcctgctc ttcctcgtcc cgctcgcgaa cgtggtggcg atgcccggcg ccgtcgtcgg
     1261 cggcacgctg ctcgtccggc gactgaccgg tgcacccgac cgctgaccgg cccgccggag
     1321 ggcccggccc cgccggaggg cgcccgcggc cctcggtccc acggtgcccg gccgctggcg
     1381 tatcctgcgc cgtcgggaca cgagcgggga ggtcgaggca tgcgggtcgg cgtgctcggg
     1441 gcgacgggca ggatgggcca ggcggtctgc cgagccgtcc tcgcggccga ggacctcgag
     1501 ctggtcgcgg cggtcgcccg caagaccggg gtgggccacg agctgcgcac ggtgatcccc
     1561 gaggcgccgc ctgacctcct ggtcggccag tacctgtccg acctcatcag cgccgaggtc
     1621 gacgtggtcg tggacttctc cgtccccgag gcgaccgtca tcgccgcccg caccctgctg
     1681 cccgagggga tccacctcgt ctcggggacg accggcctgc ccgagggggc cacggaggag
     1741 ctgcccggcc tggcccgcgc ggacgaggcc gggaacgtcg tgtgggcggc gaacttcgcc
     1801 ctcggggcgg tcctcgccat ccacttcgcg acggtcgccg cccgcttcta ccccgccgag
     1861 atcgtcgagc tgcaccacgc ggccaaggcc gacgcgccct cgggcacggc catccgcacc
     1921 gcgcacgcgg tcgcggccga gcgggcggcg accgacggcg ccgggccggg cctggccggg
     1981 cccggccggg aggtggtggc cggcgcccgc ggggccgacg tcgacggggt gcgtgtgcac
     2041 tcggtccgcc tgcccggcct cgtcgcccat caggaggtcg tgttcggcgg gcagggcgag
     2101 ctcctgaccg tccgccacga cgcgctcgac cgggccgcgt acatgccggg ggtgctgctc
     2161 gcggtccgcc aggtgccgtt gcggcccggt ctcaccgtgg ggctcgagcc gctgctcggg
     2221 ctcgcatgac cgcgggggcg gccacccggc cggcctactg ggtcgaggag ttcgacgacg
     2281 ccgagcgggc ccgcctcgag ccatggttca ccaacaccga cgggccggtg ttcgcgcttc
     2341 gcaacctccc cgagacggtc aagggggcgc tgttcgcgcg ctactcgcgc taccagggga
     2401 cgctgcgccg gctgttcctt gacgagttcg cggactcgct gcccgacgcg ccggccgtgc
     2461 cggagggagg cgagggcaag cgggcggccg agctctacga gcggatcttc ctcggctacg
     2521 gggacgactc cgtggcccag ctcggtggcg cccacgtcgc gtgcgagtgg gtctccaacg
     2581 tgctcacgaa ggtgctgcag cggccgcgcc tcgccgccta cctcgagcag tcaacgcgct
     2641 acatcgccta cgacgagccg atggattcga gcggtcggta ccgctactac cgcgaccccg
     2701 acctcgggcc cgagtacggg gcggcgatgg acgacctgtt cggcatctac tccgcggccc
     2761 tgccgcgcgt gatcgagtgg gcgtcgtcgg cgttcccgct gggcgaaggc gaggcgccgg
     2821 gcgcccatgc acgcgcgatc aaggcgaagg cgctcgacct gctgcgcggc ctgctgcccg
     2881 cc
//
LOCUS       DASPYY010000220         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1164088, whole genome shotgun sequence.
ACCESSION   DASPYY010000220 DASPYY010000000
VERSION     DASPYY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164088"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VEP73_03400"
     CDS             complement(<1..487)
                     /locus_tag="VEP73_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25515.1"
                     /translation="MTATSEADTPAPAPGRAGAGPGGGLRRRASGLAGLALLAAVAAG
                     LLGQGAYYAPVQRYVGLLVAAATVLALAAWPPARGDVRLLPVVPALALAAWALLDAAL
                     LGVPAASAGTVLELLGVVAVLLVCRRLGREDREVLLLGVIGVGLAVALAGWLGVVGRV
                     GS"
     gene            complement(713..>1656)
                     /locus_tag="VEP73_03405"
     CDS             complement(713..>1656)
                     /locus_tag="VEP73_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYT25516.1"
                     /translation="VTSGPPRAGAGHGAGGYGDPRAGGYGDRVRRALPAFFQPLEVAA
                     VLPSTMLRAAELAAGGAGEGATVVADVQTAGRGRLNRTWSAPPGSSLLLTVVLRPSLA
                     PAEAWLAAAAAGVALVDAVRVTLARAGALAAAARAPVGAGPGRQGAGTRVGLKWPNDL
                     LLDGRKAAGLLAEARVRGGRVEWVLVGMGVNVGHGAADLPAGLAGRATSVALAAGGPV
                     DRVDLLEAWAGPFLTRYRALAAGDTAAVLADYASRLDTLGREVRADLLGAGPVTGTAA
                     GLGPTGGLLVRTAAREVVEVAAGDVEHLRTAESPSRG"
BASE COUNT          213 a          702 c          608 g          133 t
ORIGIN      
        1 aggagccgac gcggccgacg accccgagcc agccggccag cgccaccgcc aggccgaccc
       61 cgatgacgcc gagcagcagg acctcccggt cctcccgccc gaggcggcgg cagacgagca
      121 gcaccgcgac gacgccgagc agctcgagca cggtcccagc gctggctgcg gggacgccga
      181 gcagagcggc gtcgagcagc gcccacgccg ccagtgccaa cgccgggacc acaggcagaa
      241 gacgaacgtc accgcgggcc ggcggccacg ccgccagcgc cagcacggtc gccgccgcga
      301 ccagcagccc gacgtagcgc tgcaccggcg cgtagtaggc gccctggccg agcaatcccg
      361 cggcgacggc ggccagcagc gccagtccgg ccagtccgct cgccctgcgc cggagccctc
      421 cgcccgggcc ggcaccggcc cgcccgggcg caggcgccgg cgtgtcagcc tcgctcgtcg
      481 cggtcaccgg gagcgccggc gcctgcggcc gcgacccgac gtcatcgggt gccacgcggc
      541 ttccgcgccg acctgccccc gcgacggaca tggcacccct cagcacgtag cggctggtcc
      601 cggcctactc ggcagaaccc ctcctgtagg gccgaagtaa catagtcgcc ccaaaggatg
      661 agaacaagac cttggtgctg aacggcacct gcgacgcgag ttcggtgccg cgtcaccccc
      721 gggacggcga ctccgcggtc cgcaggtgct ccacgtcccc cgcggcgacc tcgacgacct
      781 cccgggccgc ggtccgcacg aggaggccgc cggtcgggcc gagtccggcg gcggtgccgg
      841 tgaccgggcc ggcgccgagc aggtcggccc gcacctcgcg ccccagggtg tcgagcctgc
      901 tcgcgtagtc ggccagcacc gccgcggtgt ccccggccgc gagcgcccgg tagcgggtga
      961 ggaacgggcc ggcccacgcc tcgagcaggt cgacccggtc gaccggcccg cctgcggcca
     1021 gggcgacgct ggtggcccgg ccggccagcc cggccgggag gtcggccgcc ccgtgcccca
     1081 cgttcacccc catgccgacc agaacccact cgacccggcc gccgcgcacg cgcgcctcgg
     1141 cgagcaggcc ggccgccttg cggccgtcga ggagcaggtc gttcggccac ttgagcccca
     1201 cgcgggtgcc cgccccctgc cggcccggcc ccgcacccac gggcgcccgg gcagccgcgg
     1261 cgagcgcgcc ggcccgggcg agcgtgacac ggacggcgtc gaccagcgcg accccggccg
     1321 cggcggccgc gagccaggcc tcggcgggcg ccaggctggg ccggagcacc accgtgagga
     1381 gcagcgagct ccccggaggc gcggaccacg tccggttcag gcgcccccgg cccgccgtct
     1441 ggacgtcggc gacgaccgtc gcaccctcgc cggcgccccc cgcggccagc tcggcggccc
     1501 gcagcatcgt gctgggaagg acggccgcca cctcgagcgg ctggaagaac gcgggcagcg
     1561 cccggcggac ccggtcgccg tacccgccgg cccgggggtc gccgtagccg ccggccccgt
     1621 ggccggcgcc cgcccggggc ggcccgctcg tcacgc
//
LOCUS       DASPYY010000221         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1584898, whole genome shotgun sequence.
ACCESSION   DASPYY010000221 DASPYY010000000
VERSION     DASPYY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584898"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VEP73_03410"
     CDS             complement(<1..462)
                     /locus_tag="VEP73_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT25517.1"
                     /translation="MTARAGQLCLADVETAAARIAGRVHRTPVLTSRLLDAELGCRVF
                     AKAEHLQRVGAFKARGATNAILALDPRVRANGVIAVSSGNHAQAVALAAAEAGTTAVI
                     VMPEDSNPAKLAATRAYGAVVVTEGVTHERREQVVRELARSRSLHLVHPYDD"
     gene            complement(459..1484)
                     /locus_tag="VEP73_03415"
     CDS             complement(459..1484)
                     /locus_tag="VEP73_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HYT25518.1"
                     /translation="MIADLRSDTLTRPTPAMRRAMAGAEVGDDLWREDPTTMRLEAAV
                     AERFGREAALFVPSGTMGNQICLRLGTSPGTECLAHEDNHIFWYESGAAPLLSGVQLH
                     ALAGPRGRLAPEAVRARVRTAEYGVPTTAIAVEQTHNRGGGAVQPLADLDGLRAVADG
                     AGLHLHCDGARIWNAAVASATPLERYGALFDSLSVCFSKGLAAPVGSAVVGDRELVER
                     ARRVRQLLGGGMRQSGVLAAAALVALDEMVDRLAVDHANARRAAETLAAALPGSTDPA
                     LAETNIVLLDAGAFGTDAPTLVKRLAAAGVLAAATGPGQLRLVFHYEVTGEDADRAAR
                     AIAAAAG"
BASE COUNT          183 a          603 c          658 g          194 t
ORIGIN      
        1 gtcgtcgtag gggtgcacga ggtggagcga gcgcgacctt gccagctccc gcacgacctg
       61 ctcacggcgc tcgtgggtca ccccctcggt gaccacgacg gcgccgtagg cgcgggtggc
      121 ggcgagcttc gccgggttcg agtcctccgg catgacgatc accgcggtgg tcccggcctc
      181 ggcggcggcg agcgcaactg cctgcgcgtg gttgccggag ctgaccgcga tcaccccgtt
      241 cgcccgcacc cgtgggtcga gcgccaggat ggcgttcgtc gcccccctgg ccttgaacgc
      301 cccgacgcgc tgcaggtgct cggccttggc gaacacccgg cagccgagct cggcgtccag
      361 cagccgggag gtgagcaccg gcgtgcggtg gacccggccg gcgatgcggg cggcggccgt
      421 ctccacgtcg gcgaggcaga gctggccggc gcgggccgtc atccggcggc cgcggcgatc
      481 gcgcgggcgg cccggtcggc gtcctcgccg gtgacctcgt agtggaagac cagccgtagc
      541 tggccggggc cggtggccgc cgcgagcacg cccgcggcgg ccaggcgctt gaccagggtg
      601 ggggcgtcgg tcccgaacgc gccggcgtcg agcaggacga tgttggtctc ggcgagggcc
      661 gggtcggtgc tgccgggcag ggccgcggcg agcgtctcgg ccgcgcggcg ggcgttggcg
      721 tggtcgaccg ccagccggtc caccatctcg tcgagggcga cgagcgcggc cgcggccagc
      781 acgccggact ggcgcatccc gccgccgagg agctggcgca cccggcgggc ccgctcgacg
      841 agctcccggt cgccgaccac ggccgagccg accggcgcgg ccagcccctt ggagaagcac
      901 accgagagcg agtcgaacag cgcgccgtag cgctcgagcg gggtcgccga ggcgaccgcg
      961 gcgttccaga tgcgggcgcc gtcgcagtgg aggtgcaggc cggccccgtc ggcgacggcg
     1021 cgcagcccgt cgaggtcggc gagcggctgc acggcgccgc cgccccggtt gtgggtctgc
     1081 tcgaccgcga tcgcggtcgt cggcacgccg tactcggccg tgcggacccg cgcccgcacc
     1141 gcctccgggg cgaggcggcc cctcgggccg gccagcgcgt ggagctggac gccgctgagc
     1201 agcggggccg ccccggactc gtaccagaag atgtggttgt cctcgtgggc gaggcactcg
     1261 gtgcccgggc tggtcccgag gcgcaggcag atctggttgc ccatcgtgcc cgagggcacg
     1321 aacagggccg cctcgcggcc gaagcgctcg gccacggcgg cctcgaggcg catggtggtg
     1381 gggtcctcgc gccacaggtc gtcgccgacc tccgcgccgg ccatggcccg ccgcatcgcc
     1441 ggcgtgggcc gggtcagggt gtcggagcgg aggtcggcga tcacggcgac gattgtagcc
     1501 gcgcgcgagc cgctggtctg cgaggatcgg ggacggcgcg gccgacgccg cgcagcgccc
     1561 tcgagcatcc ccggaggttc ccatggccca ccccgtcctg cccggcgccg agccgttcac
     1621 gttcgagggc ggcgccgt
//
LOCUS       DASPYY010000222         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_601610, whole genome shotgun sequence.
ACCESSION   DASPYY010000222 DASPYY010000000
VERSION     DASPYY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601610"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1857
                     /locus_tag="VEP73_03420"
     CDS             <1..>1857
                     /locus_tag="VEP73_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYT25519.1"
                     /translation="VQGIDLSSFLQPMLAADVVRYVGEPVAVVLAEDPYAAEDAAELV
                     DLDIDEAAPVLDAQAAAQPGAPQLFGAGNVAADFTLGFGDVDAAFDRAATVVETQIEI
                     GRHTAVPLEPRALLADVDPAAGTLSIYGMTKVPVFNRDMLAGLLGMPEDLIHVHAVDA
                     GGGFGVRGEFYPEDFLVPWLATRLRRPVKWTEDRAEHLVAVNHSRQQRHRIAVAFDPA
                     GMITGLRDDIVHDNGAYCRTHGIAVPELTVTMLPGPYRIPAYRGRIRVVLTNKTPCGT
                     YRAPGRFEGTMAREQLLDVAAGRLGLDRTELRRRNLLGPGDMPHTRAMTALGTEVVLD
                     AADYPALLTAAEAEAGRLGYHDEVARGRDEGRLRGLGLALFLEKSGLGPQETADVIVG
                     ATGAVSVFSGGTSFGQGIETALAQIAADALGVSPEVVRVVSGDTARMPFGMGSWASRT
                     TVVAGNAVHAAALAVRDRARQVGARMLEVAKDDLDVHDGVVSVAGDPLASVTLAEVAW
                     ATATASQYLRPGEQAGLSARRRFDVSHMTYPYGVHIAVVEVDPGTGRVQVLRYLVAYE
                     VGRAVNPTMVEGQLRGGAAQGIGGALLEELCYDESGQPTATTFMDYRMPTAAE"
BASE COUNT          240 a          664 c          713 g          240 t
ORIGIN      
        1 gtccagggca tcgacctgag cagtttcctg cagccgatgc tggccgccga cgtggtccgg
       61 tacgtgggtg agccggtcgc ggtggtgctc gccgaggacc cctacgccgc cgaggacgcg
      121 gccgagctgg tggacctcga catcgacgag gccgcgccgg tactcgacgc ccaggccgcg
      181 gcccagcccg gcgctccgca gctgttcggc gcgggcaacg tcgcggccga cttcaccctg
      241 ggcttcggcg acgtcgacgc ggcgttcgac cgtgccgcca ccgtggtcga gacgcagatc
      301 gagatcggcc ggcacacggc ggtgccgctg gagccccgcg ccctgctggc cgatgtcgac
      361 ccggcggccg gcacgctgtc gatctacggg atgaccaagg tcccggtctt caaccgggac
      421 atgctggccg gcctgctcgg catgcccgag gacctgatcc acgtgcacgc cgtcgacgcg
      481 ggcggcgggt tcggcgtgcg gggcgagttc tacccggagg acttcctggt gccctggctc
      541 gccacccggc tgcggcggcc ggtcaagtgg accgaggacc gggccgagca cctggtcgcg
      601 gtcaaccatt cccggcagca gcggcaccgg atcgcggtgg cgttcgatcc ggccgggatg
      661 atcaccgggc tgcgcgacga catcgtgcac gacaacggcg cctactgccg cacgcacggc
      721 atcgccgtgc ccgagctgac cgtgaccatg ctgcccggcc cctaccggat ccccgcctac
      781 cgcggccgga tccgggtggt gctcaccaac aagaccccgt gcggcaccta ccgggcgccc
      841 ggccggttcg agggcaccat ggcccgcgag cagctcctcg acgtggccgc cggccggctc
      901 ggcctggacc gcaccgagct gcgccgccgc aacctgctcg ggccggggga catgccgcat
      961 acccgggcga tgaccgcgct cggcaccgag gtggtcctcg acgccgccga ctatcccgcg
     1021 ctgctgaccg cggcggaggc ggaggcgggc aggctcggct atcacgacga ggtggcccgg
     1081 ggccgggacg agggccggct gcgcggcctg ggcctagccc tgttcctgga gaagagcggg
     1141 ctgggcccgc aggagaccgc cgacgtcatc gtcggcgcga ccggggcggt cagcgtgttc
     1201 tccggcggga cctcgttcgg gcagggcatc gagacggcgc tggcgcagat cgcggccgat
     1261 gccctgggcg tcagcccgga ggtggtgcgg gtcgtcagcg gggacacggc ccggatgccg
     1321 ttcggcatgg gatcctgggc ctcccggacg accgtggtcg cgggcaacgc ggtgcacgcc
     1381 gcggcgctgg ccgtccgcga ccgcgcccgg caggtcggcg cccggatgct ggaggtggca
     1441 aaggacgacc tggacgtgca cgacggcgtg gtcagcgtgg cgggcgatcc gctcgcctcg
     1501 gtcacgctcg ccgaggtcgc ctgggcgacg gcgacggcca gccagtacct gcggccgggc
     1561 gagcaggccg ggctgtccgc gcggcggcgg ttcgacgtgt cgcatatgac ctacccgtac
     1621 ggcgtgcaca tcgcggtcgt cgaggtcgac ccgggtaccg ggcgggtcca ggtgctgcgt
     1681 tacctggtgg cctacgaggt cggccgggcc gtcaacccga ccatggtcga gggccagctg
     1741 cggggcgggg cggcgcaggg catcggcggc gcgctcctgg aggagctctg ctacgacgaa
     1801 tccggccagc cgacggcgac cacgttcatg gactaccgga tgccgacggc ggccgag
//
LOCUS       DASPYY010000223         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_181400, whole genome shotgun sequence.
ACCESSION   DASPYY010000223 DASPYY010000000
VERSION     DASPYY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181400"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1751
                     /locus_tag="VEP73_03425"
                     /pseudo
     CDS             208..1751
                     /locus_tag="VEP73_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
     gene            1748..1948
                     /locus_tag="VEP73_03430"
     CDS             1748..1948
                     /locus_tag="VEP73_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HYT25520.1"
                     /translation="MSAPFLRVERGHLTAEELAALTVALLAAAPVAPLTPAPRPQPRP
                     AHWRRLERTVAFRPGHSWQLSR"
     gene            1957..>2849
                     /locus_tag="VEP73_03435"
     CDS             1957..>2849
                     /locus_tag="VEP73_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYT25521.1"
                     /translation="MQKVLIANRGEIAVRVARACRDAGLGSVAVYAEPDRDAPHVRAA
                     DEAYALGGATPGGSYLDAGKLLNAARVAGADAVHPGYGFLSENADFAQAVLDAGLTWI
                     GPPPAAIRALGDKVTARHIAQRVGAPLAAGTPDPVADAAQIVAFAQRHGLPVAIKAAF
                     GGGGRGLKVVRTMEEIPELFESAVREAVAAFGRGECFAERYLDRPRHVETQCLADRHG
                     TVVVVSTRDCSLQRRHQKLVEEAPAPFLTPAQHDELVRASKAILAEAGYAGAGTCEFL
                     VGRDGTISFLEVNTRLQVEHPV"
BASE COUNT          406 a         1070 c         1021 g          352 t
ORIGIN      
        1 gggtgctgca gaccgagctc ggcctggagc cgacgccgga ggtgcagcga ctgcagaccg
       61 aggtcctcgg cgggggccgc accggcggcg cgccgcgcct gctgcgcgcc atctgacgac
      121 gcctccggcg ggacttgacc acggctcgag tcccgccgcc cagactggcg acgccgacgg
      181 cccctaccat tcctggaggc gaatccggtg ttacagcgcg tggacgaact caacgacctc
      241 aagcggcggg tggcggccgg ccccgacccg gaggccaccc ggcgccagca cggccggggc
      301 aagctgaccg ctcgcgagcg catcgacgcg ctcctcgacg agggctcctt ccaggaggtc
      361 gacgggctgc ggcggcaccg ggcgaccggc ttcggcctgg aggccaagcg gccgcacacc
      421 gacggtgtcg tcaccggctg gggcaccgtc cacggccgtg gcgtcttcgt gtacgcccac
      481 gacttccgca tcttcggcgg cgccctcggc gaggcgcacg cgcagaagat ccacaaggtg
      541 atggacctgg cgatcgccac cggcgccccg ctcgtgagcc tcacgacggg gccggcgccc
      601 gcatccagga gggggtcacc gcgctcgccg gctacggcgg catcttcgag cgcaacaccc
      661 gcgcgtccgg ggtgataccg cagatcagcg tcatgctcgg tccgtgcgcc ggcggggcgg
      721 cctacagccc ggcattgacc gacttcgtgt tcatggtgcg cggcacgtcg cagatgttca
      781 tcaccgggcc cgacgtcgtg caggcggtca ccggcgagcg ggtcacccac gacgcgctcg
      841 gcggcgcgga cgtgcacgcg gagcgctccg gcgtggccca cttcgtctac gacgacgagg
      901 cgacctgcct gaaggaggta cgctacctgc tgtcgatgct gccgcagaac aaccgcgagc
      961 agccgccggc gatgctcggc gcggaccccg ccgaccggcg cagcgaggcg ctctacgacc
     1021 tggtgccggc cgacggcaac cggccgtacg acatgcgggc cgtcatcgag gagatcgtcg
     1081 acgacggggc gcacctcgag gtgcacgagc gctgggccgg caacgtcatc tgcaccctgg
     1141 cccggctcga cggccgggcg gtcggcatcg tcgccaacca gccgcagtcg ctggccggcg
     1201 tgctcgacat cgccggttcc gagaaggcgg cccgcttcgt gcagacgtgc gactcgttca
     1261 acatccccat cgtcacgctc ctggacgtgc ccggcttcct gcccggcgtc gcccaggagc
     1321 acggcggcat catccggcac ggggcgaagc tgctgtacgc ctactgcaac gccaccgtgc
     1381 cgcggatctc ggtcgtgctg cgcaaggcgt acggcggggc gtacatcgtc atggactcgc
     1441 gctccatcgg cgccgacctg tccctggcgt ggccgaccaa cgagatcgcc gtgatgggcg
     1501 ccgagggggc cgcgaacgtc gtcttccggc gcgagatcgc cgccgcggac gatccggagg
     1561 acatgcgcaa gcagctgacg aaggagtacc gcatgaacct catgcacccc taccacgcag
     1621 ccgagcgcgg gctcgtcgac gacgtgatcg acccggccga cacccggccg gtgctcatcc
     1681 gcagcctgac catgctcacg accaagcacg ccgacctgcc gatgcgcaag cacggcaacc
     1741 caccgcagtg agcgcgccgt tcctgcgcgt cgagcgggga cacctgaccg ccgaggagtt
     1801 ggcggcgctg acggtcgcgc tcctcgccgc agcgccggtc gcgccgttga ccccggcgcc
     1861 acgtccgcag ccgcgcccgg cgcactggcg gcggctggag cgcacggtcg cgttccggcc
     1921 cggccacagc tggcagcttt cccggtagga gacgagatgc agaaggtcct gatcgccaac
     1981 cgcggcgaga tcgcggtccg ggtcgcccgc gcgtgccggg acgccgggct cggcagcgtc
     2041 gccgtgtacg cggaaccgga ccgcgacgcc ccgcacgtac gagccgccga cgaggcctac
     2101 gcgctgggcg gcgcgacgcc cggcgggtcc tatctggacg ccggcaagct gctgaacgcg
     2161 gcgcgggtcg cgggcgcgga cgcggtgcac cccggctacg gcttcctgtc cgagaacgcc
     2221 gacttcgccc aggcggtgct cgacgccggc ctgacgtgga tcggcccgcc gccggcggcg
     2281 atccgcgcgc tcggcgacaa ggtgaccgcg cggcacatcg cccagcgcgt cggggcccca
     2341 ctggcagccg gcacccccga cccggtggcc gacgccgcgc agatcgtggc gttcgcccag
     2401 cggcacgggc tgccggtcgc catcaaggcg gcgttcggcg gcggcgggcg cggcctgaag
     2461 gtcgtccgca ccatggagga gatcccggag ctgttcgagt ccgcggtccg cgaggcggtc
     2521 gccgcgttcg ggcgcggcga gtgcttcgcc gaacggtacc tggaccggcc ccggcacgtg
     2581 gagacgcagt gcctcgccga ccggcacggc accgtcgtcg tcgtgtccac aagggactgt
     2641 tcgctgcagc ggcggcacca gaagctcgtc gaggaggcgc cggcgccgtt cctgaccccg
     2701 gcccagcacg acgaactcgt ccgcgcctcc aaggcgatcc tcgccgaggc cggctacgcc
     2761 ggcgccggca cgtgcgagtt cctcgtcggc cgcgacggca cgatctcctt cctcgaggtg
     2821 aacacccggc tgcaggtcga gcacccggt
//
LOCUS       DASPYY010000224         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2709539, whole genome shotgun sequence.
ACCESSION   DASPYY010000224 DASPYY010000000
VERSION     DASPYY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709539"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VEP73_03440"
     CDS             <1..626
                     /locus_tag="VEP73_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT25522.1"
                     /translation="KTVSGLLRPRRGSIHLGDRPVHGLSPRAVLELGVVHVPQDRSLF
                     PLMSVWENVLMGAYVVRDRAAVNRRIAEVAELFPIVGRRRHARAGSLSGGEQKLVELA
                     RALMLDPVLVLMDEPSIGLDPRARAQVFDSIRSMNESGRTVLLVEQNARSGLAAAHRA
                     SVMEAGVVKLEGTGAELLADPAVARLYLGATTRQGLASPARTDGTTS"
     gene            638..1519
                     /locus_tag="VEP73_03445"
     CDS             638..1519
                     /locus_tag="VEP73_03445"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT25523.1"
                     /translation="MASVGFVGLGAMGGRIARRLLDAGHDVTGWNRTRSKVAWLAEAG
                     LTLAGSPREVAAASDVTFTMVTDTGALHAVVRGEDGLLAGLGPDKVYVDMSTVSPVAS
                     RELAAEVATVGASMLDAPVSGSIATLEQGRLSVMVGGDRATFERVLPLLEAIGPKVIH
                     VGSSGQAVLMKTAVNLSLAVQLAAFSEGLLIAEKGGIPREVAVEALLASVVASPSLAY
                     RAPFVFQLPEEAWFDVNMMQKDMLLALETGRALDVPMPTTAATNELLTAARGLGLEKQ
                     DFAVIYHTLARMSGLER"
     gene            1579..>1881
                     /locus_tag="VEP73_03450"
     CDS             1579..>1881
                     /locus_tag="VEP73_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HYT25524.1"
                     /translation="MQVDWEERVDPRRLHDYRVGRARQALAASDLGAVLVFDMNNVRY
                     LTSTHIGEWARDKLITFALLTRTGEPIVWDFGSAARHHSLYCPWLAPGSSRAGLNGL"
BASE COUNT          250 a          699 c          683 g          249 t
ORIGIN      
        1 tgaagacggt cagcggcctg ctgcgcccgc ggcggggctc catccacctc ggcgaccggc
       61 cggtccacgg cctctccccg cgcgccgtgc tcgagctcgg ggtcgtgcac gtcccgcagg
      121 accggagcct cttcccgctc atgtcggtct gggagaacgt cctcatgggc gcctacgtcg
      181 tgcgggaccg ggcggccgtc aaccggcgca tcgccgaggt ggccgagctg ttcccgatcg
      241 tcgggcggcg ccgccacgcc cgcgcgggct cgctgtccgg cggcgagcag aagctcgtcg
      301 agctcgcccg cgccctcatg ctcgacccgg tcctcgtcct catggacgag ccgtcgatcg
      361 ggctcgaccc gcgcgcccgg gcgcaggtgt tcgacagcat ccgctcgatg aacgagagcg
      421 gccgcaccgt gctcctggtg gagcagaacg cccgctcggg ccttgccgcc gcccaccgcg
      481 cctcggtgat ggaggcaggc gtggtgaagc tcgaggggac cggtgccgag ctgctcgccg
      541 acccggcggt ggcccggctc tacctcggcg ccaccacgcg gcagggcctc gcttccccgg
      601 cccgcacgga cggcacgacg agctgagagg agcgaccatg gccagcgttg gattcgtcgg
      661 gctgggcgcg atgggcggcc gcatcgccag gcgcctgctc gacgccgggc acgacgtcac
      721 gggctggaac cgcaccaggt ccaaggtggc ctggctggcc gaggcgggcc tcacgctcgc
      781 gggcagcccg cgcgaggtgg cggcggcgtc cgacgtgacc ttcacgatgg tgaccgacac
      841 cggcgcgctg cacgcggtgg tccgggggga ggacggcctg ctcgccggcc tcggcccgga
      901 caaggtctac gtcgacatga gcacggtgag cccggtcgcc agccgcgagc tcgccgccga
      961 ggtggccacg gtcggggcca gcatgctcga cgccccggtc tccggcagca tcgcgaccct
     1021 ggagcagggc cggctgtcgg tcatggtcgg cggcgaccgc gccacgttcg agcgggtcct
     1081 gccgctcctc gaggcgatcg ggcccaaggt catccacgtc ggctcgagcg ggcaggccgt
     1141 gctgatgaag acggccgtca acctgagcct cgcggtccag ctcgccgcgt tcagcgaggg
     1201 tctgctgatc gccgagaagg gcgggatccc gcgcgaggtc gcggtcgagg cgctgctggc
     1261 cagcgtggtc gcctcgccgt cgctcgccta ccgggcgccg ttcgtgttcc agctccccga
     1321 ggaggcctgg ttcgacgtca acatgatgca gaaggacatg ctgctggcgc tcgagaccgg
     1381 ccgcgcgctc gacgtgccca tgccgaccac ggcggccacc aacgagctgc tcaccgccgc
     1441 gcgcgggctc ggcctcgaga agcaggactt cgccgtcatc taccacaccc tggcccgcat
     1501 gtccgggctc gagcgctgaa cgggggagag gacgtgaccg ccaccaccac ccggcccgtg
     1561 ctgccggcac caggccacat gcaggtcgac tgggaggagc gggtcgaccc gcgccggctg
     1621 cacgactacc gcgtcggccg ggcacggcag gccctcgcgg cgagcgacct cggcgccgtg
     1681 ctcgtgttcg acatgaacaa cgtccgctac ctgaccagca cccacatcgg cgagtgggcg
     1741 cgcgacaagc tcatcacctt cgccctgctg acccggaccg gcgagccgat cgtgtgggac
     1801 ttcggctcgg cggcacgaca ccacagcctg tactgcccgt ggctcgcgcc gggcagctcg
     1861 cgggccgggc tgaacgggct g
//
LOCUS       DASPYY010000225         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_40386, whole genome shotgun sequence.
ACCESSION   DASPYY010000225 DASPYY010000000
VERSION     DASPYY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40386"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEP73_03455"
     CDS             complement(<1..319)
                     /locus_tag="VEP73_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25525.1"
                     /translation="MQPSKASVLVFLGNSSVGLIGVALWRYAPVVLPAVLLPVATLHL
                     AYGSTVREAEERDRFRHLCFGPASDADLAALRLLGDSPCPVVGSGYRAPAGWREVLAR
                     ILGL"
     gene            597..1223
                     /locus_tag="VEP73_03460"
     CDS             597..1223
                     /locus_tag="VEP73_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYT25526.1"
                     /translation="MSTLPRRLPRLVPLAGVLAALGAVLVALAPVPVAAQAAQAQGAK
                     EVDGTAQNKWVPATVNIQPGQSVTFKVASGATHPVGSGTSPTAKDNRFDTSKCQLAQM
                     TKVGDSCTVKFPKAGTYPFFCEVHFALGMTGTIQVGKAVGGPTATTAAAAGGGVVPTP
                     STGPPTAPGRPAIYYAGYGLVAGGVLFALFTFMSYVRYAPGFRRGDRR"
     gene            1348..2433
                     /locus_tag="VEP73_03465"
     CDS             1348..2433
                     /locus_tag="VEP73_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYT25527.1"
                     /translation="MVSTAAPTEAPDIVDWNLAARVGRRVAGGPSLLVPDAWSREFTD
                     VSTTADRAVAEFTGLGGELPPPLAVPLDRGQWVEANLATLRRVLKPVAEKVAARKAWR
                     EQGPVPSAMRSGTRALTGAQAGTLLGYVGQRVLGQYDLPVPGPEEIADGTVWYVVPNV
                     AGTERRYGFRPAHFRLWIALHETAHRRQFRGVPWLPGHIQGLLDEYLGSVEVDDEALR
                     RVGRRAQSVARRAIAGDRIELLDFLLTEEQRAVVDRMQATMSVLEGHGEWVMDQLGAR
                     LVPGHERMHKTLRDRRENASPADRFFQQLLGFRQKLDQYSNGERFVRALYERGGMATV
                     NLVFTAPEALPTKDEVARPDRYLARAG"
BASE COUNT          317 a          944 c          902 g          291 t
ORIGIN      
        1 agagcccgag gatccgggcg agcacctcgc gccagcccgc cggggcccgg tagccgctcc
       61 cgacgaccgg gcacggggag tccccgagca gccgcagcgc ggcgaggtcg gcgtcgctgg
      121 ccgggccgaa gcagaggtgc cggaagcggt cgcgctcctc cgcctcgcgg acggtcgacc
      181 cataggcgag gtgcagcgtc gccaccggca ggagcaccgc ggggagcacg acgggcgcgt
      241 accgccagag cgcgaccccg atgaggccga ccgacgagtt gccgaggaag acgaggacgg
      301 acgccttcga gggctgcacc ctgcgacacc tccaagctgg cgatcggagt cggcgccgcg
      361 cgccggccgg cctcccgccc gccgggccgg atcgtggggt cgtgcggtcc cagctccctc
      421 atcggagccg ccccagagcc gcttgatagc ccttgtgagc tggcccgatg gggtcaggct
      481 ccttgccacg ggcaggggcc gtggtgctag gatcgcgcgg ctgccgagcc gggcggtcag
      541 ctcggcccgg ccacgcgaac ggcccggcca cccgaaccag gagcgcacgc aggcccatgt
      601 cgaccctccc tcgccgcctt ccccgtctcg tcccgctggc cggcgtcctg gccgccctcg
      661 gcgccgtgct ggtcgcgctg gctcccgtcc ccgtcgccgc ccaggccgcc caggcgcagg
      721 gcgccaagga ggtcgacggc acggcccaga acaagtgggt acccgccacc gtcaacatcc
      781 agcccgggca gtcggtgacc ttcaaggtcg cgagcggtgc gacccacccg gtcggctccg
      841 ggacctcccc gaccgccaag gacaaccggt tcgacacgtc gaagtgccag ctcgcccaga
      901 tgacgaaggt gggcgactcc tgcaccgtca agttcccgaa ggccggcacc tacccgttct
      961 tctgcgaggt gcacttcgcc ctcggcatga ccgggaccat ccaggtcggc aaggccgtcg
     1021 gcggccccac cgcgacgacc gcggcggcgg ccggcggcgg cgtggtcccg acgccgtcca
     1081 ccggtccgcc gacggccccg ggccgaccgg cgatctacta cgccgggtac ggcctggtgg
     1141 ccggcggggt gctgttcgcc ctgttcacct tcatgagcta cgtccgctac gcgcccgggt
     1201 tccggcgcgg cgaccgccgc tgacgcgcgg gcggccaggg gccgccggcg cgcggaagcc
     1261 ggcgtcgggc cggccggcac cccggacgag cggccggttc caggcgtagc ctggagccgc
     1321 tcgccgtctc gccgtcctgg agccaccgtg gtctcgaccg cagccccgac cgaagccccc
     1381 gacatcgtcg actggaacct ggccgcccgc gtgggccggc gcgttgccgg cgggccgtcc
     1441 ctgctcgtgc cggacgcttg gtcgcgtgag ttcaccgacg tctcgaccac cgccgaccgg
     1501 gccgtcgccg agttcaccgg cctcggcggc gagctgccgc cgccgctcgc cgtgccgctc
     1561 gaccgcggcc agtgggtcga ggccaacctg gccacgctgc ggcgcgtgct caagccggtg
     1621 gcggagaagg tggcggcccg caaggcgtgg cgggagcagg gcccggtgcc gtcggcgatg
     1681 cgctccggca ccagggcgct gaccggcgcc caggccggca ccctgctcgg ctacgtcggc
     1741 cagcgcgtgc tcggccagta cgacctgccc gtgcccggcc cggaggagat cgccgacggc
     1801 accgtctggt acgtggtgcc gaacgtcgcg gggaccgagc ggaggtacgg gttccggccg
     1861 gcgcacttcc ggctctggat cgccctgcac gagacggccc accggcgcca gttccgcggc
     1921 gtcccgtggc tgccagggca catccagggc ctgctcgacg agtacctcgg ctcggtcgag
     1981 gtcgacgacg aggcgctgcg gcgcgtcggc cgccgcgccc agtccgtggc ccgccgggcg
     2041 atcgccgggg accggatcga gttgctcgac ttcctgctca ccgaggagca gcgcgccgtc
     2101 gtcgaccgca tgcaggccac catgagcgtg ctcgagggcc acggcgagtg ggtgatggac
     2161 cagctcgggg cgcggctcgt ccccgggcac gagcggatgc acaagaccct gcgcgaccgg
     2221 cgcgagaacg cgagccccgc cgaccgtttc ttccagcagc tgctcggctt ccggcagaag
     2281 ctcgaccagt actcgaacgg ggagcggttc gtccgcgcgc tctacgagcg gggcggcatg
     2341 gcaaccgtca acctggtgtt cacggccccc gaggcgctcc cgaccaagga cgaggtcgcc
     2401 cgccccgacc gctacctggc ccgggcgggc tagcggggcg accggtggcg cggg
//
LOCUS       DASPYY010000226         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2710087, whole genome shotgun sequence.
ACCESSION   DASPYY010000226 DASPYY010000000
VERSION     DASPYY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710087"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..3042)
                     /gene="uvrA"
                     /locus_tag="VEP73_03470"
     CDS             complement(52..3042)
                     /gene="uvrA"
                     /locus_tag="VEP73_03470"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYT25528.1"
                     /translation="MGADPRFPPDHEDTTLSATDRLVIRGAREHNLKNVNLDLPRNAL
                     VVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLSPAIS
                     IDQKSTSKNPRSTVGTITEIYDYLRLLYARIGQPHCPKCGRPIARQTPDQIVDQILAL
                     PAGTRFQVLAPVVRGRKGEYEALLRSFSAKGFARARVDGEVRDLSEPIRLAKTYKHDI
                     EIVVDRLVARGPDADERSRRRLADSVETAVGLADGLVLAELVDEHRELQFSTHLACLY
                     DNLSFEDLAPRNFSFNAPYGACPDCTGLGVKQEVDPELVVPNPALSVAGGAISPWQWG
                     RGNTEYFDRLLAAVADACGFDVATPWAELTAEQRRVLLYGADEQVYVRYKNRYGRSRA
                     YHTTYEGVVPYLNRRYAEAESDALRERVESYMREVPCPTCKGARLRPESLAVTVAGRN
                     IAEVAGMSVGETAAFFRSLELSDRDAFIAARVLKEINARLGFLLDVGLDYLTLDRGSA
                     TLAGGEAQRIRLATQIGSGLTGVLYVLDEPSIGLHQRDNHRLIETLVRLRDLGNTLIV
                     VEHDEATIKNADYLVDIGPGAGEHGGKIVAEGSLADLERVEESLTGQYLSGKRSIPVP
                     VSRRRPGERWLELTGVREHNLKDLTARLPLGVFTCITGVSGSGKSTLINDVLLKALMQ
                     AVYRSRTSPGRFLSLSGAEHLDKVIDVDQSPIGRTPRSNPATYTGVFDHIRKLFAQTS
                     EAKVRGYQPGRFSFNVKGGRCEACSGDGTIKIDMQFLPDIYVPCEVCKGKRYNRETLE
                     VHYKGHTVADVLEMSVEEGLALFANIPPIARHLETLNDVGLGYIKLGQPAPTLSGGEA
                     QRVKLASELAKRATGRTLYVLDEPTTGLHFEDVAKLLGVLHRLVDTGNSVIVIEHNLD
                     VVKTADWILDLGPEGGSGGGEIIAQGPPERVARAPASYTGRFLAGALGVPLAAAARDG
                     SGGAGGVPAPPERARAPRRKAAAG"
     gene            3194..3853
                     /locus_tag="VEP73_03475"
     CDS             3194..3853
                     /locus_tag="VEP73_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017545293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT25529.1"
                     /translation="MASFDPAHVVQGGPPHHYPDGALRVVKLHVGPWENNAYLLHDPQ
                     TNEALLVDAANEADRLLSELLAGVALVGVVTTHRHPDHIQALPAVLRKHDVWNGAHPA
                     DADAIAAEVGVTPDRVLEHGDTVGVGGWAVTVLHTPGHTEGSISFKLPSSQVLTGDAL
                     FPGGPGKTEGRAAFDQAIASIERHLFTLPAETRVSPGHGDDTSVGTEAPHLEEWRARG
                     W"
     gene            complement(3891..>4071)
                     /locus_tag="VEP73_03480"
     CDS             complement(3891..>4071)
                     /locus_tag="VEP73_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYT25530.1"
                     /translation="GAVAGKTGTAEFGTGNPLPTHAWFIGFRGDLAFAVVVEGGGVGG
                     EVAAPLAGRFLAALG"
BASE COUNT          551 a         1439 c         1523 g          558 t
ORIGIN      
        1 accggcggcg gcgggccggg cggccgggcg ccgccgcggc gtcagccggc gtcagccggc
       61 ggcggccttc ctcctgggcg cgcgggcgcg ctcgggcgga gccggcacgc cgccggctcc
      121 gcccgagccg tcccgggcag cggcggcgag cgggacgccg agcgcgccgg ccaggaaccg
      181 gccggtgtag gaggcggggg cgcgggccac ccgctcgggc ggaccctggg cgatgatctc
      241 gccgccgccg ctgccgccct ccgggccgag gtcgaggatc cagtcggcgg tcttgacgac
      301 atcgaggttg tgctcgatca cgatcaccga gttgccggtg tcgaccagcc ggtggagcac
      361 cccgaggagc ttcgcgacgt cctcgaagtg caggccggtg gtgggctcgt cgagcacgta
      421 gagggtccgg ccggtcgccc gcttggccag ctcgctggcc agcttgaccc gctgcgcctc
      481 accgccggac agcgtgggcg ccggctggcc gagcttgatg tagccgaggc cgacgtcgtt
      541 cagcgtctcg aggtggcggg cgatcggcgg gatgttggcg aacagggcga ggccctcctc
      601 gaccgacatc tccagcacgt cggcaaccgt gtggcccttg tagtggacct cgagcgtctc
      661 gcggttgtag cgcttgccct tgcagacctc gcaggggacg tagatgtcgg gcaggaactg
      721 catgtcgatc ttgatcgtcc cgtccccgga gcaggcctcg cagcggccgc ccttgacgtt
      781 gaacgagaac cgccctggct ggtagccgcg gaccttggcc tcgctggtct gggcgaacag
      841 cttgcggatg tggtcgaaca cgccggtgta ggtggccggg ttggaccgcg gcgtgcgccc
      901 gatcggcgac tggtcgacgt cgatgacctt gtcgaggtgc tcggcgccgg acagcgacag
      961 gaaccggccg ggggaggtcc gcgagcggta gaccgcctgc atgagcgcct tgagcaggac
     1021 gtcgttgatg agggtcgact tgcccgaccc ggacacgccg gtgatgcagg tgaacacgcc
     1081 gagcggcagg cgggcggtga ggtccttgag attgtgctcg cgcacgccgg tcagctcgag
     1141 ccagcgctcg cccggccgcc gccggctgac cggcaccggg atggagcgct tgcccgacag
     1201 gtactgcccg gtcagggact cctcgacccg ctcgaggtcg gccaggctgc cctcggcgac
     1261 gatcttgccg ccgtgctcgc cggcgcccgg cccgatgtcg accaggtagt cggcgttctt
     1321 gatggtggcc tcgtcgtgct cgaccacgat gagggtgttg ccgaggtcgc ggagccggac
     1381 gagcgtctcg atgagccggt ggttgtcccg ctggtggagc ccgatcgagg gctcatcgag
     1441 gacgtacagc accccggtga gccccgagcc gatctgggtg gcgagccgga tgcgctgcgc
     1501 ctccccgccg gcaagggtgg ccgacccccg gtccagcgtc aggtagtcga gcccgacgtc
     1561 gagcaggaag ccgagccgcg cgttgatctc cttgagcacc cgcgcggcga tgaaggcgtc
     1621 ccggtccgac agctcgagcg agcggaagaa cgcggccgtc tcccccaccg acatgccggc
     1681 cacctcggcg atgttccgcc cggccacggt cacggcaagg ctctcgggcc gcagccgggc
     1741 gcccttgcag gtcgggcagg gcacctcgcg catgtaggac tcgacccgct cgcgcagcgc
     1801 gtcggactcg gcctccgcgt agcggcggtt gaggtagggc acgacgccct cgtaggtcgt
     1861 gtggtaggcg cggctgcggc cgtagcggtt cttgtacctg acgtagacct gctcgtccgc
     1921 cccgtacagc agcacccgcc gttgctcggc ggtcagctcg gcccacgggg tcgcgacgtc
     1981 gaagccgcag gcgtcggcga ccgccgccag cagccggtcg aagtactcgg tgttgcccct
     2041 gccccactgc cacgggctga tcgcgccccc tgcgaccgac agggccgggt tggggacgac
     2101 cagctccggg tcgacctcct gcttgacgcc gaggccggtg cagtccgggc aggcgccgta
     2161 gggagcgttg aacgagaagt tccgcggggc caggtcctcg aacgacaggt tgtcgtacag
     2221 gcaggcgagg tgggtggaga actggagctc gcggtgctcg tccaccagct cggcgaggac
     2281 gagcccgtcg gccaggccga cggcggtctc cacggagtcg gccagccgcc gccgcgaccg
     2341 ctcgtcggcg tcggggccgc gggccacgag ccggtcgacc acgatctcga tgtcgtgctt
     2401 gtaggtcttg gcgagccgga tcggctccga caggtcgcgc acctcgccgt cgacccgggc
     2461 gcgggcgaac cccttggccg agaagctgcg cagcagcgcc tcgtactcgc ccttgcggcc
     2521 ccgcacgacc ggggccagga cctggaaccg ggtgcccgcc gggagggcga ggatctggtc
     2581 gacgatctgg tcgggggtct ggcgggcgat cggccggccg cacttcgggc agtggggctg
     2641 gccgatgcgg gcgtacagca gccgcaggta gtcgtagatc tcggtgatcg tgccgacggt
     2701 cgatcggggg ttcttcgagg tcgacttctg gtcgatcgag atggccggcg acagcccctc
     2761 gatgaagtcc acgtcgggct tctccatcag cccgaggaac tggcgggcgt aggccgacag
     2821 cgactcgacg tagcgccgct gcccctcggc gtagatcgtg tcgaaggcga gcgacgactt
     2881 cccggagccg gacagcccgg tgaagaccac cagcgcgttg cgcgggaggt cgaggttgac
     2941 gttcttcagg ttgtgctcgc gggcaccccg gatgacgagg cggtcggtgg cgctcagggt
     3001 cgtgtcctcg tggtcagggg ggaagcgcgg gtcggccccc atattgtaag gacgggtgtg
     3061 acaacgggcc tccgcggccc ggcaccaggg cggcaggtgc cttccgccgc ctcaggagtg
     3121 acaatgcgcg agtgctcccg tgcattcccg gccggcgacc cgagccggcg gcccagagcc
     3181 gaggaggcag cacatggcca gcttcgaccc cgcccacgtc gtccagggcg gcccgccgca
     3241 ccactacccc gacggcgccc tgcgcgtcgt caagctgcac gtcgggccct gggagaacaa
     3301 cgcctacctg ctccatgacc cccagaccaa cgaggccctg ctggtcgacg cggccaacga
     3361 ggccgaccgc ctgctgtcgg agctgctcgc cggggtggcg ctggtcggcg tcgtgaccac
     3421 ccaccgccac cccgaccaca tccaggcgct cccggccgtg ctccgcaagc acgacgtctg
     3481 gaacggggcg caccccgccg acgccgacgc gatcgcggcc gaggtcgggg tgacccccga
     3541 ccgggtgctc gagcacggcg acacggtcgg cgtcgggggc tgggccgtca cggtcctgca
     3601 caccccgggc cacaccgagg gcagcatctc gttcaagctc ccgtcgagcc aggtgctgac
     3661 gggcgacgcc ctgttcccgg gcggcccggg caagaccgag ggccgggccg ccttcgacca
     3721 ggccatcgcg tcgatcgagc ggcacctgtt caccctgccg gccgagaccc gggtctcccc
     3781 tggccacggc gacgacacct cggtggggac cgaggccccc cacctcgagg agtggcgcgc
     3841 ccggggctgg tagccgcgga cgccacgccc ggccctggcc gtccggcggg tcagccgagc
     3901 gcggccagga accggcccgc gagcggcgcg gcgacctcgc cgccgacccc gccgccctcg
     3961 accacgacgg cgaacgcgag gtcgccccgg aagccgatga accaggcgtg ggtcggcagg
     4021 gggttgccgg tgccgaactc ggccgtgccg gtcttgccgg ccaccgcgcc g
//
LOCUS       DASPYY010000227         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2289384, whole genome shotgun sequence.
ACCESSION   DASPYY010000227 DASPYY010000000
VERSION     DASPYY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289384"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEP73_03485"
     CDS             complement(<1..409)
                     /locus_tag="VEP73_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT25531.1"
                     /translation="MNDTSVRRAWPGVIERYRGRLPVGPSTPVVTLLEGGTPLVPAPV
                     LSARTGCDVHLKVEGANPTGSFKDRGMTVAVSKAVEAGSRAVLCASTGNTSASAAAYA
                     ARAALTCAVLVPEGKVATGKLAQALVHGATVLAV"
     gene            complement(406..1761)
                     /locus_tag="VEP73_03490"
     CDS             complement(406..1761)
                     /locus_tag="VEP73_03490"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYT25532.1"
                     /translation="MVACRSRRGDETAGGHVGGPVRVGMLGCGVVGTGVARLLEQHAT
                     LVADRAGAGIELQRVAVRDVTRKRDPAVDPTRLTGLAAEVVADPEVDVVVEVMGGIEP
                     ARSLIVEALRLGKPVVTANKELLATHGHELFDAADAAGVDLKFEAAVAGAIPIIHPLK
                     ESLAGDRVRRVLGIVNGTTNYVLWRMASTGASYAEALAEATDLGYAEPDPSADVEGFD
                     AAAKCAILASLAFNTRVVADDVYREGITDVTAADIAAAARMGYTVKLLAIAEEVGGQV
                     SARVHPAMVPDAHPLAGVTEAYNAIFVEGDAIGQLMFYGRGAGGLPTGSAVLGDLVDV
                     VRNRAQGARGVGCTCFYERRVRGMEDIDTQYYLALDVADRPGVLAQVAAVFADHDVSI
                     RSVLQRGKGDQAQLVLVTHLAREGAMRATLARLSKLEVVGSLASVMRVEGEEADEVMA
                     R"
BASE COUNT          227 a          699 c          651 g          236 t
ORIGIN      
        1 ccacggccag cacggtcgcc ccgtggacga gcgcctgcgc gagcttgccg gtggcgacct
       61 tgccctccgg gaccagcacc gcgcaggtga gagcggcccg ggccgcgtag gcggccgccg
      121 acgccgaggt gttcccggtc gaggcgcaca gcaccgcccg gctgcccgcc tccaccgcct
      181 tggagaccgc gacggtcatg ccccggtcct tgaacgaccc ggtcgggttc gcgccctcga
      241 ccttcaggtg gacgtcgcag ccggtgcggg ccgacaggac cggggccggc acgagcggcg
      301 tgcccccctc gagcagggtg acgacggggg tgctcggccc gaccggcagg cggccgcggt
      361 agcgctcgat gacgccaggc cacgcccggc gcacgctggt gtcgttcatc gggccatcac
      421 ctcgtccgcc tcctcgccct cgacccgcat gacgctcgcg aggctgccga ccacctcgag
      481 cttggagagc ctggccagcg tcgcccgcat ggcgccctcc ctggccaggt gggtgaccag
      541 cacgagctgg gcctggtcac ccttgccccg ctgcaggacc gaccggatcg agacgtcgtg
      601 gtcggcgaac acggccgcca cctgggccag cacgccgggc cggtcggcca cgtcgagggc
      661 cagatagtac tgcgtgtcga tgtcctccat cccacggacc cgccgctcgt agaagcaggt
      721 gcacccgacc ccgcgggcgc cctgtgcccg gttgcggaca acgtccacga ggtcgccgag
      781 cacggccgag ccggtcggca gccccccggc gccacgcccg tagaacatga gctgcccgat
      841 cgcgtcacct tcgacgaaga tcgcgttgta cgcctcggtg accccggcga gcgggtgggc
      901 gtccgggacc atggccgggt ggacgcgggc gctcacctgg ccgcccacct cctcggcgat
      961 cgcgagcagc ttgaccgtgt agcccatccg ggcggccgcg gcgatgtcgg ccgcggtcac
     1021 gtcggtgatc ccttcccggt agacgtcgtc cgcgacaacc cgggtgttga acgccaggct
     1081 ggcgaggatc gcgcacttcg cggcggcgtc gaagccctcg acgtcggcgg acgggtccgg
     1141 ctcggcgtag ccgaggtcgg tcgcctcggc cagcgcctcg gcgtagctgg ccccggtcga
     1201 cgccatccgc cagaggacgt agttcgtcgt gccgttgacg atcccgagga cgcggcggac
     1261 gcggtcgccc gcgagcgact ccttcagcgg gtggatgatg gggatggcac cggccaccgc
     1321 cgcctcgaac ttgaggtcga ccccggcggc gtcggcggcg tcgaagagct cgtggccgtg
     1381 ggtcgcgagc agctccttgt tggcggtgac gaccggcttg ccgagccgca gcgcctcgac
     1441 gatcaggctg cgggcgggct cgatgccccc catgacctcg accacgacgt cgacctcggg
     1501 gtccgcgacg acctcggccg cgaggccggt cagccgggtc gggtcgacgg cggggtcgcg
     1561 cttgcgggtc acgtcccgca ccgcgacccg ctgcagctcg atgcccgcgc ctgcccggtc
     1621 ggcgaccaag gtggcgtgct gctcgagcag ccgggccacc ccggtgccga ccacgccgca
     1681 gcccagcatg ccgaccctga ccggtccgcc cacgtgccct cccgccgttt cgtctccccg
     1741 ccggcttcgg caggcgacca tcttgcccga cgccaggggt cgacggcacg ccgggcccgc
     1801 ccgccgggcg gcg
//
LOCUS       DASPYY010000228         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2710461, whole genome shotgun sequence.
ACCESSION   DASPYY010000228 DASPYY010000000
VERSION     DASPYY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710461"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VEP73_03495"
     CDS             complement(<1..622)
                     /locus_tag="VEP73_03495"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYT25533.1"
                     /translation="MTEVFRLCDDYMTRWAALDPVAAGMVGLSEAFGAATDYGPDGCA
                     AREELIATTLAALGAAPVTSAADRLAASVLRERLEAQLAWHQAGEPLRLLRTPIGQLS
                     AIRDSVDLLPRDGDESWRNIAARLAAFPAMFASWRASLNAGLDRGLTAARRQAVEAAA
                     AADQYAGTHDVLVASYGDGPLAAGLAAAAEQAHGAYLEMARYLREDY"
     gene            complement(645..>1953)
                     /locus_tag="VEP73_03500"
     CDS             complement(645..>1953)
                     /locus_tag="VEP73_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HYT25534.1"
                     /translation="ADGKLHGAAPAPYRAIDLVAAARGRTRDEGFAAEDEALADLLLS
                     EELRAGLYAFDLTQKRARHAAGAPDPSLARPVTKIGVVGAGLMAAQIALLFARRLEVP
                     VVLTDLDQARLDAGLGHVRAEVARLRERGRISADAAARLTALVTGSLTKDAFADADFV
                     IEAVFEEMAVKQRVLAEVEAVVRPDCVLATNTSSLSVTEMASGLAHPERVAGFHFFNP
                     VAMMPLVEVVRGAATDDATAATALAVGGRAKKNCVLVKDAPSFVVNRLLTRFLGEILA
                     SVDEGTPIEVAEHAPDPLGLPMSPFFLLQLVGPAVALHVGETLAAAYPGRFAVSPKLR
                     ALVAAGKTAVYRPDLTVDESVAAILDGGDRPSTVEEVRERALEALAEEIRLMLDEGVV
                     AGPEDIDLCMILGAGWPFHLGGITPYLDRTGIAEKVTGTRFRL"
BASE COUNT          260 a          758 c          690 g          245 t
ORIGIN      
        1 cgtagtcctc gcgcaggtag cgggccatct ccaggtaggc gccgtgcgcc tgctccgcgg
       61 ccgcggcgag cccggcggcc agcgggccgt ctccgtagga ggccaccagc acgtcgtgcg
      121 tcccggcgta ctggtcggcc gcggccgccg cctcgacggc ctgcctgcgc gcggcggtca
      181 ggccgcggtc aagcccggcg ttcaggctcg cccgccagct ggcgaacatc gcggggaacg
      241 ccgccaaccg cgccgcgatg ttccgccagc tctcgtcgcc gtcgcggggc agcaggtcca
      301 cgctgtccct gatcgcactg agctgcccga tcggggtgcg gagcagccgg agcggctcgc
      361 cggcctgatg ccaggccagc tgggcctcca gccgttctcg caggacggag gctgccagcc
      421 ggtccgccgc cgaggtcacc ggcgccgccc caagcgcggc cagcgtcgtg gcgatcagtt
      481 cctcgcgcgc cgcgcatccg tccggcccgt agtcggtggc cgcgccgaac gcctcgctga
      541 gcccgaccat cccggccgcg accggatcca gcgccgccca ccgggtcatg tagtcatcgc
      601 aaagccggaa cacctcggtc atgggttcac cctacgaccg gaggttagag gcggaaccgg
      661 gtgcccgtca ccttctccgc gatcccggta cggtccaggt acggcgtgat cccgcccagg
      721 tggaacggcc agccggcgcc caggatcatg cacaggtcga tgtcctcggg cccggcgacg
      781 acgccctcgt cgagcatcag ccggatctcc tcggccagcg cctccagggc ccgctcgcga
      841 acctcctcca ccgtggacgg gcggtcaccg ccgtccagga tggccgccac cgactcgtcg
      901 accgtgaggt cgggccggta gacggcggtc ttacccgccg ccaccagggc gcggagcttg
      961 ggcgacaccg cgaaccggcc cgggtacgcc gcggccagcg tctcccccac gtgcagcgcg
     1021 actgccgggc cgaccagctg gagcaggaag aacggggaca tgggcaggcc gagcgggtcg
     1081 ggcgcgtgct cggccacctc gatcggcgtt ccctcgtcga cactggcgag gatctcgccg
     1141 aggaaccggg tcagcaggcg gttcaccacg aaggacggcg cgtccttgac cagcacgcag
     1201 ttcttcttcg cccgcccgcc gacggcgagc gcggtggctg ccgtcgcgtc atcggtggcg
     1261 gcgccgcgga ccacctcgac cagcggcatc atggccaccg ggttgaagaa gtggaagccc
     1321 gctacccgct cgggatgcgc cagccccgac gccatctcgg tcaccgacag cgaggaggta
     1381 ttcgtggcca gcacgcagtc cggccggacc accgcctcga cctcggcgag cacccgctgc
     1441 ttgaccgcca tctcctcgaa cacggcctcg atcacgaagt cggcgtcggc gaacgcgtcc
     1501 ttggtcagcg acccggtgac cagcgcggtc agccgcgccg cggcgtcggc gctgatccgg
     1561 ccgcgctcgc gcagcctggc cacctcggcc cgcacgtgac cgagcccggc gtccagccgg
     1621 gcctggtcca ggtcggtcag caccacgggc acctcgagcc gccgcgcgaa cagcagcgcg
     1681 atctgcgcgg ccatcagccc ggcgcccacc accccgatct tggtgacggg ccgggctagc
     1741 gacgggtcgg gcgcaccggc cgcgtgccgg gcccgcttct gggtcaggtc gaaggcgtag
     1801 agcccggccc gcagttcctc gctcagcagc aggtcggcga gggcctcgtc ctcggcggcg
     1861 aacccctcgt cgcgggtccg gccgcgggcc gcggccacca ggtcgatggc ccggtagggc
     1921 gccggggcgg cgccgtgcag cttgccgtcg gcg
//
LOCUS       DASPYY010000229         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2007983, whole genome shotgun sequence.
ACCESSION   DASPYY010000229 DASPYY010000000
VERSION     DASPYY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007983"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VEP73_03505"
     CDS             complement(<1..415)
                     /locus_tag="VEP73_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25535.1"
                     /translation="MALPLAMAIVTVLAGPAAAHGVGVGLRPTNYRTTVTSVTPPLPG
                     LAVHVLEAGDKLQLVNRTGQEVLVLGYNSEPYLRVGPPGAGVYENERSPTTYSNRFFT
                     APSKLPPDLDPSATPEWRRIGTGPAVAWHDHRSHFG"
     gene            complement(472..1284)
                     /locus_tag="VEP73_03510"
     CDS             complement(472..1284)
                     /locus_tag="VEP73_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25536.1"
                     /translation="MRTSPRSAGTGSSAPAPGAAAARRAALLGLAVVLLYLAGSGVSG
                     SLSPLARRPLLDGLAPPTRYRWVKPPPVLAQGNKQPTPMRFTLDLGPKGSTVGAFSTQ
                     DGQFNLVFGEGAFPPSPGQSKVAFEVVPLDPATVPPASGGLLAAGNAYRFTAAYQPSR
                     RAVGKLAAETNAGVVYPLLTVPVASAAGHVLLYSPDGRSAWTRLQSIDAPGVHQVSAR
                     IPGTGYYLAAIPPASAAPAPATLADKVRGALPAVAVVVLVAGAGALYFRRRR"
     gene            complement(1281..>1606)
                     /locus_tag="VEP73_03515"
     CDS             complement(1281..>1606)
                     /locus_tag="VEP73_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25537.1"
                     /translation="KAAYGTSMTAPALAGFSGAWALFHYVLPNARSTRPADVAAAARA
                     ARVPRGALPNGSGLEFGRPGTIDAGANVHAASVIWQWAGLNRREVVWPPQFATAPFVP
                     VPIAR"
BASE COUNT          193 a          570 c          666 g          177 t
ORIGIN      
        1 ccccgaagtg gctccggtgg tcgtgccagg ccaccgcggg gccggtgccg atccggcgcc
       61 actcgggcgt ggccgacggg tccaggtcgg gcgggagctt gctcggcgcg gtgaagaacc
      121 ggttcgagta ggtcgtcggc gagcgctcgt tctcgtagac accggccccc gggggcccga
      181 cgcgcaggta gggctccgag ttgtagccga ggacgagcac ctcctggccc gtccggttga
      241 ccagctggag cttgtctccc gcctcgagga cgtggacggc gaggccgggg aggggcgggg
      301 tgacggacgt gacggtcgtg cggtagttgg tcggccgcag gccgaccccg acgccgtggg
      361 ccgccgccgg gccggcgagc acggtcacga tcgccatggc cagggggagc gccaccagga
      421 cccgccgcgc ggcccgccgc ggcggcgacg ggggtcgcgc tgcgggccgg ctcaccggcg
      481 ccgccggaag tagagcgcgc cggcgcccgc gaccaggacg accacggcca ccgccggcag
      541 cgcgcccctc accttgtcgg ccagggtcgc gggcgcaggc gccgccgagg cgggcgggat
      601 ggccgccagg tagtagccgg tgccggggat gcgggcgctg acctggtgga cgcctggcgc
      661 gtcgatgctc tgcagcctgg tccaggcgct cctgccgtcc ggggagtaca gcaggacgtg
      721 gcccgccgcg ctcgcgaccg gcacggtgag cagcgggtag accaccccgg cgttggtctc
      781 ggccgccagc ttgccgaccg cccggcgcga cggctggtag gcggcggtga agcggtaggc
      841 gttgccggcg gcgagcagcc cgccagaggc cggggggacg gtcgccgggt cgagcggcac
      901 gacctcgaac gccaccttgg actggccggg gctcggcggg aaggcgccct cgccgaagac
      961 gaggttgaac tgcccgtcct gggtcgagaa ggcgccgacg gtggacccct tggggccgag
     1021 gtccagcgtg aagcgcatcg gggtaggctg cttgttgccc tgggccagca ccggcggcgg
     1081 cttcacccag cggtacctgg tcgggggggc caggccgtcg agcagcggcc gcctggcgag
     1141 cgggctcagc gacccgctga cgcccgagcc cgcgaggtag agcagcacga cggcgaggcc
     1201 gaggagggcc gcccgccggg cggcggcggc cccgggtgcc ggcgccgagc tccccgtccc
     1261 ggcgctgcgg ggggaggtcc tcatcgggcg atcggcacgg ggacgaacgg cgccgtcgcg
     1321 aactgcggcg gccagacgac ctcgcggcgg ttcaggccgg cccactgcca gatgacgctc
     1381 gcggcgtgca cgttggcccc ggcgtcgatc gtccctggcc ggccgaactc gagcccgctg
     1441 ccgttcggca gggcgccgcg cggcacccgg gcggcgcggg cggccgcggc cacgtccgcg
     1501 ggcctcgtcg acctcgcgtt ggggagcacg tagtggaaca gcgcccaggc ccccgagaag
     1561 ccggcgagcg ccggggcggt catggaggtc ccgtacgccg ccttgt
//
LOCUS       DASPYY010000230         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2569956, whole genome shotgun sequence.
ACCESSION   DASPYY010000230 DASPYY010000000
VERSION     DASPYY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569956"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1070
                     /locus_tag="VEP73_03520"
     CDS             123..1070
                     /locus_tag="VEP73_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYT25538.1"
                     /translation="MNGADALDAYSRAVVGVAERLAPAVASLRVQRRTRRGRMPVGAG
                     SGVVLTHDGFILTSAHVVAGPASDGRAAFTDGRELRFSVLGRDPLSDLAIVRAEDGGL
                     SPAELGDADELRVGQLVVAIGNPHGFEGSVTAGVVSALGRALPAPAGRRPARIIENVI
                     QTDAALNPGSSGGALADGQGRVVGINTALAGIGLGLAVPINATIRQIIGALMSEGRVR
                     RAFVGVAGGPRPLPPRVRARWGDRTGVEVVEVVADSPADRAGLREGDLILEVEGSPVT
                     SAADLQRMMTAELIGRSVNVAVWRAGGEASVQLVPAELS"
     gene            1085..1555
                     /locus_tag="VEP73_03525"
     CDS             1085..1555
                     /locus_tag="VEP73_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HYT25539.1"
                     /translation="MAGNDPVPTLYEWAGGTSAFERLTAAFYERVRQDDLLQPLFANM
                     PDDHPRHVAIWLAEVFGGPGAYTDEHGGYPHMVSKHLGLAISERQRSRWVQLIGLAAD
                     DVGLAADPEFRSAFMAYVEWGTRIALANSQPGAAPPPQAPVPRWGWGEAPPQVP"
BASE COUNT          235 a          601 c          685 g          247 t
ORIGIN      
        1 gagcgccagg tcgaggttga gctggccggg gcgagggcgt gatggcgggc gtggaccatg
       61 cgccggcacc tcctgactcg gcccccgtcg agaccgcgcc ggtggggcgc cccagcacgg
      121 aggtgaacgg ggcggacgcg cttgacgcct actcacgcgc ggtggtggga gtcgccgagc
      181 ggctcgcgcc cgcggtggcc agcctgcgcg tccagcggcg cacgcggcga ggccggatgc
      241 cggtcggtgc cggcagcggc gtggttctca cccatgacgg cttcatcctc acctcggccc
      301 acgtggtcgc cggtccggca agcgacggcc gggcggcgtt caccgacggg cgcgagcttc
      361 gcttctcggt gctcggccgc gacccgctgt cagacctggc gatcgttcgc gccgaggacg
      421 gcggcctcag cccggcggag ctgggcgacg cggacgagct gcgggtcggc caactggtgg
      481 tggcgatcgg caacccgcac ggcttcgagg gctcggtcac ggccggggtt gtctcggcgc
      541 tgggacgggc cctgccggcg ccggcgggcc gccgaccggc gcgcatcatc gagaacgtga
      601 tccagaccga cgccgcgctc aacccgggaa gctcgggagg cgcgctggcc gacgggcagg
      661 gccgagtggt cggcatcaac acggcgctcg ccggcatcgg gctcggcctc gccgtgccga
      721 tcaacgcgac catccgccag atcatcggcg ccctgatgag cgaggggagg gtgcgccgcg
      781 ccttcgtcgg cgtcgccggc ggtcctcgac cgctgccgcc acgggtgcgg gcgcgatggg
      841 gcgaccgcac cggcgtcgag gtggtggagg tcgtggccga cagtccggcc gatcgtgccg
      901 gtctacgcga gggcgacctg atcctggagg tggaaggaag ccccgtgacc agcgccgcgg
      961 acctgcagcg gatgatgacc gcggagctga tcggccgctc ggtcaacgtg gcggtctggc
     1021 gagcgggcgg cgaggccagt gtccagctgg tgccggccga gctgagctag aaggggttct
     1081 gatcatggcc gggaacgatc cggttccgac gctctacgaa tgggccgggg gcacgtccgc
     1141 cttcgagcgt ctgaccgcag ccttctatga gcgggtgcgc caggacgacc tgcttcagcc
     1201 gctgttcgcc aacatgcccg acgaccaccc gcgacacgtg gccatatggc tggcggaggt
     1261 gttcgggggt cccggggcct acacggacga gcacggcggc tacccgcaca tggtctcgaa
     1321 gcacctcggg ttggcgatca gcgagcgcca gcggtcacgg tgggtacagc tgatcggcct
     1381 ggccgcggac gacgtgggac tcgcggctga tcctgagttc aggtccgcct tcatggccta
     1441 cgtggagtgg ggcacccgca tcgcgctcgc caactcccag ccgggggcgg ctccgccgcc
     1501 gcaggcgccg gttccccgct ggggctgggg cgaggcgccg ccgcaggtgc cgtgagcgcg
     1561 ttcggcgact aggagcgccg gggccccggc aagagactca caggctgagc ggcgcgaaag
     1621 tgcgccggca gcgccacagc catcgaagcc cacgggacgg ttgtggtgtt tgccgcccat
     1681 atgacgctca atgccgcacg cgtcgttcgc ggtccctcga gtgtggcgtt gtcggcgccg
     1741 ttccgtccat cccatgtgcg aacatgtg
//
LOCUS       DASPYY010000231         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2289783, whole genome shotgun sequence.
ACCESSION   DASPYY010000231 DASPYY010000000
VERSION     DASPYY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289783"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1787
                     /locus_tag="VEP73_03530"
     CDS             189..1787
                     /locus_tag="VEP73_03530"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HYT25540.1"
                     /translation="MEDKELVVTARRRVAAVAAAVVALVAVGVVVAVRPGGRLPTLAG
                     PAAAGPAGAPAARAGSAITWVAAENQRPGTTAWRITHLGTPDAIEGYADRASATWGER
                     VHVYVSTTAPSFHVEAYRMGYYHGLGARLVWRSAAIPGRRQPPPVRTPGTNLLATHWP
                     PALALPLTPTWPQGDYLLKLTAATGQRYVPLTIRDDTSHAALLIQNAVTTWQAYNAWG
                     GASLYHGPHGNPATRSRVVSFDRPYDPPGLGAGDFLGNELPLVRLVEQHGLDVTYATD
                     LDLHRHPERLLAHRALVSLGHDEYWSTRMRRGAETARAHGVNLAFLGANAVFRHIRLQ
                     PSPLGPDREEVNYKPWSAGDDPAWRSDPGEVTTDWRQPPLNDPESRLLGAQYQCNPVH
                     ASGVVVDPAAWPLAGTGVHAGMRLAGLVGGEYDGVRPGAPRPAGVQVLLHAPVRCRGP
                     SAADTTYYTVPGGGAGVFDAGTGSWVCQLAGACAQGRNDPVTARVVGAVTLNLLRGFA
                     QGPAGRHHPSRPNLTRLHVVPNGL"
     gene            1832..>2528
                     /locus_tag="VEP73_03535"
     CDS             1832..>2528
                     /locus_tag="VEP73_03535"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HYT25541.1"
                     /translation="MVPVIARRRGRSLGAALALLALLAGCGHSTKSPSAASSTGTAAP
                     ATAAPGTAAPPRPVVFPLTGLPAPGAAAASRAALSIKVENTPEARPQAGLDAADVVTE
                     ELVEGGITRFLATFQSRDAATVGPVRSARPVDAALLRQLGGGLFGYAGAAAGEIAPVK
                     AESTAVLVGAEQAPGAYYRIATRPAPHNLYTSTDRLYQAAARLGAHRGPPPALFGFTT
                     QAPTGARPVRAVAV"
BASE COUNT          310 a         1080 c          839 g          299 t
ORIGIN      
        1 ctaccgccca gtggggtcat ggcagccctc taaactggct gcatgccggc gttcagctca
       61 cgttcccttc acgattccgt ctctagagtc ggcgagggtt cccggaccgc ggccgaggac
      121 gagagccgac gggcgcctgg accgtcggct ctccggcagc ccaccccgat gccagggagg
      181 cgccgcctgt ggaggacaag gagctcgtgg tgaccgcgcg tcgccgggtg gcggcggtgg
      241 cggctgccgt ggtggcgctg gtggccgtcg gggtggtcgt ggcggtccgg ccgggtgggc
      301 gccttcccac cctggcaggc ccggccgcgg ccggcccagc cggggcaccg gctgccaggg
      361 caggctccgc gatcacctgg gtggccgccg agaaccagcg ccccggcacc accgcgtggc
      421 ggatcaccca cctcggcacc cccgacgcga tcgagggcta cgccgaccgc gccagcgcca
      481 cctggggcga gcgggtccac gtctacgtct ccaccaccgc ccccagcttc cacgtcgagg
      541 cctaccgcat gggctactac cacggcctgg gggcccggct ggtctggcgc tcggcggcga
      601 ttccaggccg ccgccagcca cccccggtgc ggaccccggg caccaacctg ctcgccaccc
      661 actggccccc cgcgctcgcc ctgcccctca cccccacctg gccccaaggc gactacctcc
      721 tcaagctcac cgccgccacc gggcagcggt acgtccccct gaccatccgc gacgacacca
      781 gccacgccgc cctgctgatc cagaacgcgg tcaccacctg gcaggcctac aacgcctggg
      841 gtggcgccag cctctaccac ggcccgcacg gcaaccccgc cacccgctcc cgggtggtct
      901 ccttcgaccg cccctacgac cccccggggc tcggcgcggg cgacttcctc ggcaacgagc
      961 tccccctggt ccgcctggtc gaacagcacg gcctggacgt cacctacgcc accgacctcg
     1021 acctccaccg gcatccggag cggctcctgg cccaccgcgc gctggtctcc ctcggccacg
     1081 acgagtactg gtccacccgg atgcgccgcg gcgccgagac cgcccgcgcc cacggcgtca
     1141 acctcgcctt cctgggcgcc aacgccgtct tccgccacat ccgcctgcaa ccctcgccgc
     1201 tcggccccga ccgcgaggag gtcaactaca agccctggtc cgcgggcgac gacccggcgt
     1261 ggcggagcga cccgggcgag gtcaccaccg actggcgcca acccccgctc aacgaccccg
     1321 aaagccgcct gctcggcgcc cagtaccagt gcaacccggt ccacgcctca ggggtcgtgg
     1381 tcgacccggc cgcctggccg ctggccggga ccggggtcca cgcgggcatg cggcttgcgg
     1441 ggctggtcgg cggcgagtac gacggggtcc ggccgggcgc gccccgcccg gccggggtgc
     1501 aggtcctgct gcacgccccg gtccgctgcc gcgggccgag cgccgccgac accacctact
     1561 acactgtccc tggcggcggg gccggggtgt tcgacgccgg gaccgggtcc tgggtctgcc
     1621 agctcgccgg cgcctgcgcc caaggacgga acgacccggt gaccgcccgg gtggtcggtg
     1681 cggtcaccct gaacctgctc agagggttcg cccaagggcc agccggccgc caccacccgt
     1741 cccgacccaa cctcacccgc ctccacgtcg tcccgaacgg cttgtgacca gcatgccggc
     1801 ctcaccacct ggggaggagg ctcacccaac catggtcccc gtcatcgccc gccgccgtgg
     1861 ccggtccctc ggtgccgcgc tggccctcct cgccttgctg gccgggtgcg gccacagcac
     1921 caagtcgccg agcgcggcca gctcgaccgg caccgcggcg ccggccacgg cggcgccggg
     1981 cacggccgcc ccgccccggc cggtggtctt cccgctcacc gggctgccag caccgggcgc
     2041 ggccgcggcg tcccgggccg ccctctcgat caaggtcgag aacaccccgg aggcgcgtcc
     2101 gcaggccggg ctcgacgcgg ccgacgtggt caccgaggag ctcgtcgagg gcgggatcac
     2161 ccgcttcctt gcgaccttcc agtcccgcga cgccgccacg gtcgggccgg tccggtcggc
     2221 ccggccggtc gacgccgccc tcctgcgcca gctcggtggc gggctgttcg gctacgccgg
     2281 cgccgcggcc ggggagatcg ccccggtcaa agccgagtcg accgcggtcc tggtcggggc
     2341 cgagcaggcg ccgggcgcct actaccggat cgccacccgt cccgccccgc acaacctcta
     2401 cacctccacc gaccggctct accaggccgc ggcccggctc ggcgcccacc gtggaccgcc
     2461 ccccgccctg ttcggcttca caacccaagc gccgaccggc gcccgcccag tccgggccgt
     2521 ggccgtgg
//
LOCUS       DASPYY010000232         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2149157, whole genome shotgun sequence.
ACCESSION   DASPYY010000232 DASPYY010000000
VERSION     DASPYY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149157"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VEP73_03540"
     CDS             <1..562
                     /locus_tag="VEP73_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HYT25542.1"
                     /translation="LISVMGIEKLIPTWRDFEVFLQLLPRSSTGERMNPYTSFWTGVH
                     QADGPREFHLVLLDNGRSRVLADRVGRQTLNCIRCSACLNVCPVKIDIPEVLVHLRAQ
                     VVEHEPNAHRVPDPEAVAMEAMAWVFDDRRRYELAQKLARVGQLPLARSGRIRRLPAA
                     LSGWTEMRDAPAVPARTFRDWWRSRR"
     gene            600..1247
                     /locus_tag="VEP73_03545"
     CDS             600..1247
                     /locus_tag="VEP73_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HYT25543.1"
                     /translation="MTSAREEILQRVRTALGDVPDGERPEDVPVERGYRTQSTGDPGE
                     RVARFAERVADYKATVHRVGADELPDALARACEARQVKRLVVPPDLPEAWRPADAELL
                     VDGQLASEVLDAADGVLTGCVLGIAETGTIVLDGGPRQGRRAITLLPDYHLCVVEEQQ
                     IVGSVPEAVAALTGPAAGEHRPITFVSGPSATSDIELNRVEGVHGPRTLEVVIVG"
     gene            complement(1332..2297)
                     /locus_tag="VEP73_03550"
     CDS             complement(1332..2297)
                     /locus_tag="VEP73_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYT25544.1"
                     /translation="MRELEPAQPGTADPLAELLELADAYVLRSKAPATLKAYATDWRH
                     FSDWCSARGLAALPADVKTVAAYLAAHGGRLAVTTLRRRLSSISQIHQAAGHRTPTAT
                     AEVRLVWQGIVRTHGRRPARQAEPAVTDTLRAMVATLDGGLLGRRDRALLLIGFAGFL
                     RRSELVALDVADVVEVPEGLEVLLHRSKTDQEGEGAVLPIPYGAHEATCPVRAWRAWV
                     TAAGIAEGPAFRGVDRHGNVATTRLSDKTVARAVKRAAERAGLDTAAFSGHSLRAGLA
                     VAAARAGVAEELIMEQGRWKSPAVARSYVRKGQLWRDVAAAQVGL"
BASE COUNT          316 a          942 c          949 g          311 t
ORIGIN      
        1 tctgatcagc gtgatgggca tcgagaagct gatccccacg tggcgggact tcgaggtgtt
       61 cctgcagctc ctgccgcgct cctcgacggg ggagcgcatg aacccctaca cgtcgttctg
      121 gaccggggtg caccaggccg acgggccgcg cgagttccat ctcgtcctgc tcgacaacgg
      181 ccgctcgcgg gtgctggccg accgggtcgg ccggcagacg ctcaactgca tccgctgctc
      241 ggcctgcctc aacgtctgcc cggtcaagat cgacatcccc gaggtgctcg tccacctgcg
      301 ggcccaggtc gtggagcacg agccgaacgc ccaccgggtg cccgacccgg aggcggttgc
      361 catggaggcg atggcgtggg tgttcgacga ccggcgccgg tacgagctcg cgcagaagct
      421 cgcccgggtc ggccagcttc cgctcgcccg gtcgggcagg atccggcggc tcccggccgc
      481 actgtcgggc tggaccgaga tgcgcgacgc gcccgccgtg ccggcgcgga cgttccggga
      541 ctggtggcgc agccgccgat gaccccgacc ccacacctgc acacggaaag gcgttcgtgg
      601 tgacgagtgc cagggaggag atcctgcagc gcgtccggac ggccctcggg gacgtcccgg
      661 acggcgagcg ccccgaagac gtcccggtcg agcgcggcta ccgcacccag agcaccgggg
      721 acccggggga gcgggtggcc cggttcgccg agcgggtggc cgactacaag gcgacggtgc
      781 accgcgtcgg cgcggacgag ctgccggacg cgctcgcccg cgcgtgcgag gcgcggcagg
      841 tcaagcggct ggtcgtccct cccgacctgc ccgaggcgtg gcggccggcc gacgccgagc
      901 tgctcgtcga cgggcagctc gccagcgagg tgctcgacgc cgccgacggg gtcctgaccg
      961 gctgcgtcct cgggatcgcg gagaccggca ccatcgtgct cgacggcggc cccaggcagg
     1021 gccggcgggc catcaccctg ctccccgact accacctctg cgtcgtcgag gagcagcaga
     1081 tcgtcggctc ggtgcccgag gccgtcgccg cgctcaccgg gcccgcggca ggcgagcacc
     1141 ggccgatcac gttcgtgtcc ggcccgtcgg cgacctccga catcgagctg aaccgggtcg
     1201 agggcgtgca cggcccgcgc accctcgagg tcgtgatcgt cggctgacgc cgcttcggtg
     1261 ctgtccgcat ggtgcccgcc ggccgaccgc aggcgcgcca catccttgcg gatgcggtgg
     1321 gccgagaccc atcacagccc gacctgggcg gctgcgacgt cgcgccagag ctgccccttg
     1381 cggacgtagg agcgggccac cgccggggac ttccagcgtc cctgctccat gatcagctcc
     1441 tcggcgacgc cggcccgggc cgcggcgacg gccagcccag cccgcaggct gtggccggag
     1501 aaggcggccg tgtccaggcc ggcccgctcc gcggcgcgct tgaccgcgcg ggcgaccgtc
     1561 ttgtcggaca gccgggtcgt cgcgacgttg ccgtggcggt cgacgccccg gaacgcggga
     1621 ccctcggcga tgcccgcggc ggtgacccac gcccgccagg cgcgcacggg acaggtcgcc
     1681 tcgtgggcgc cgtaggggat cggcagcacc gccccctcgc cctcctggtc ggtctttgac
     1741 cggtgcagca ggacctcgag gccttcgggc acctcgacca cgtcggcgac gtcgagcgcg
     1801 acgagctcgg agcggcgcag gaacccggcg aagccgatca gcagcagggc ccggtcgcgc
     1861 cggccgagca ggccgccgtc gagcgtggcg accatcgccc gcagcgtgtc ggtgaccgcc
     1921 ggctccgcct gcctggccgg ccggcggccg tgcgtgcgga cgatgccctg ccagaccagg
     1981 cgcacctccg cggtcgcggt cggggtgcgg tgcccggccg cctggtggat ctgggagatg
     2041 gaggagagcc gccgccgcag cgtggtcact gccagccggc cgccgtgggc ggccaggtag
     2101 gcggccaccg tcttgacgtc ggcggggagg gcggcgagac cccgtgccga gcaccagtcc
     2161 gagaagtggc gccagtcggt cgcgtacgcc ttgagcgtcg cgggcgcctt cgaccgcagc
     2221 acgtacgcgt ccgcgagctc gaggagctcg gcgagcgggt cggcggtgcc cggctgcgcc
     2281 ggttccagct cccgcatctg cgcgaccccc agacggcccg aggccggtga aggcgtccgg
     2341 ccctctctgt cccgtaacga ctgccgcggg acgctaccac ctcggccgga cagcggcgcc
     2401 cggggcggcc cgggtcagcg gacggggcgc ctcagaggag cggctcgggc ccgagctcgc
     2461 cgctgccctc gtcgccgaag gcgatcccga ggccccgggc cgtgcggatg atctcgtg
//
LOCUS       DASPYY010000233         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1447702, whole genome shotgun sequence.
ACCESSION   DASPYY010000233 DASPYY010000000
VERSION     DASPYY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447702"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VEP73_03555"
     CDS             complement(<1..480)
                     /locus_tag="VEP73_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYT25545.1"
                     /translation="MLHVEQTADNCIKCNICNTVCPVSAVTDLFPGPKYEGPQGQRFR
                     AGVDTGESPDISLDYCSGCGLCTQACPEGVMVAEINTVARTRIVEERGGLSLRDNLIS
                     RPSLLGALARPVAPLANLLMANRLVRALVEKVLGVHRDAAMPHFSAVSFRSWARSHPS
                     "
     gene            complement(529..1797)
                     /gene="glpB"
                     /locus_tag="VEP73_03560"
     CDS             complement(529..1797)
                     /gene="glpB"
                     /locus_tag="VEP73_03560"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpB"
                     /protein_id="HYT25546.1"
                     /translation="MRSDVVVIGAGPAGLAVAALLAEAGRQVRVIARGNGFTHWGPGA
                     VDVLGRVGGQPVDQPVEAIERLPGDHPYRIAGVDTLRDGLDWFRRTAAAAGLDHEGGL
                     ERNRRQVTALGTLRPTCLLPAPAARELSGTVAVCNFAGFRDFSTALCARGLERAGLST
                     KVGVVDLPPWDHDRYFTAVELARAIDDDAFRAGVAAGLARAADGADVAVVPAVLGLRR
                     GHEPWADLERSAGLPIVEAAIVPPSVPGLRLYAAWRARLEELGVHWQFGFPATGVERE
                     GDRVTAVRSEGASRTIVTRCNEVVLATGGVAGHGIEANRDGTLAEVVAGLPVEGFADR
                     TAFVSSRFLGDHPIGAAGVRVDAELRPVHPSGTPVYANVRCVGGLLAGHDPTVEGSRE
                     GVALATATRVAGLLIPALAETGGGGRRTRP"
     gene            complement(1797..>2792)
                     /locus_tag="VEP73_03565"
     CDS             complement(1797..>2792)
                     /locus_tag="VEP73_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT25547.1"
                     /translation="DVGELAGVPIHMIPGKGVMVVMASRYVRGVVNACRKPADGDIIV
                     PQHEVAILGTTSVQVPDPDDITVDPADVDRMVDMCAQMVPSIAHGRVLRSFAGSRPLY
                     KPPATGGGDGDAADTRAVSRTFTVLDHASRDGLENMLSIVGGKLTTCRQMAEHLVDRL
                     AARMGVTVGCTTATEVLPGADHGPHRLAGPLASVERAGAYGELVCECELVTRGQVWSA
                     VAGGLGELEDLRRQLRLGYGPCQAAFCAWRAAGILCEYASTEADAESRLPDPSAALER
                     FLEERWRGQVASVWGDQARQALLNQAIYRGVFDMEPSGERDEAAAAAATGSGVTG"
BASE COUNT          350 a         1039 c         1071 g          332 t
ORIGIN      
        1 ggacgggtgg gaccgcgccc aggagcggaa gctgaccgcc gagaagtgcg gcatggcggc
       61 gtcccggtgc acgccgagca ccttctcgac cagggcgcgg acgagccggt tcgccatgag
      121 gaggttcgcg agcggggcca ccggcctggc cagcgcgccc agcaggctcg gccggctgat
      181 caggttgtcg cggagcgaca ggccgccccg ctcctccacg atcctggtgc gggcgacggt
      241 gttgatctcg gcgaccatga cgccctctgg gcacgcctgg gtgcacaggc cacagccgga
      301 gcagtagtcg agcgagatgt cgggcgactc gccggtgtcc acgccggccc ggaagcgctg
      361 cccctgcggt ccctcgtact tggggccggg gaagaggtcg gtgaccgccg agacggggca
      421 gacggtgttg cagatgttgc acttgatgca gttgtcggcg gtctgttcca cgtggagcac
      481 ggcgtcctcc ggtcacggtc ggggccaggg gcgcgaggcc cgggatggtc agggcctcgt
      541 gcgccgcccg ccgccccctg tctcggcgag ggccgggatc agcaggccgg cgacccgcgt
      601 cgcggtggcc agggcgaccc cctcgcggga cccctcgacg gtcgggtcgt ggccggccag
      661 caggccgccg acgcagcgga cgttggcgta gacgggcgtg cccgagggat gcaccggccg
      721 cagctccgcg tccacccgga cgccggccgc cccgatcggg tggtcgccga ggaaccggga
      781 cgagacgaag gccgtgcggt cggcgaaccc ctcgaccggg agaccggcga ccacctcggc
      841 cagggtgccg tcccggttgg cctcgatccc gtgcccggcg acgccgccgg tcgcgaggac
      901 cacctcgttg cagcgcgtca cgatggtccg cgaggcgccc tcgctgcgga ccgcggtgac
      961 gcggtcgccc tcccgctcca ccccggtcgc cgggaagccg aactgccagt ggacgcccag
     1021 ctcctcgagc ctggcccgcc aggccgcgta caggcggagc ccgggtacgg acggcggcac
     1081 gatggccgcc tcgacgatcg gcaggccggc gctgcgctcg aggtcggccc agggctcgtg
     1141 gccgcgccgc aggccgagca cggcgggcac caccgcgacg tcggccccgt ccgcggccct
     1201 ggcgagcccg gccgcgacgc cggcccggaa ggcgtcgtcg tcgatcgcgc gggccagctc
     1261 gacggcggtg aagtagcggt cgtggtccca ggggggcagg tccacgaccc ccaccttggt
     1321 cgagaggccg gcgcgctcga ggccgcgggc gcacagggcg gtggagaagt cccggaaccc
     1381 ggcgaagttg cacaccgcca ccgtcccgga gagctcgcgc gctgccggcg cgggcagcag
     1441 gcaggtcggc cgcagggtgc cgagcgcggt cacctggcgc cggttgcgct cgagaccgcc
     1501 ctcgtggtcg aggccggccg cggcggcggt gcggcggaac cagtcgagcc cgtcgcgcag
     1561 cgtgtcgacc ccggcgatac ggtaggggtg gtccccggga agccgctcga tcgcctccac
     1621 gggctggtcg accggctggc cgccgacccg gccgagcacg tcgaccgcgc ccggccccca
     1681 gtgggtgaag ccgttgcctc gggcgatgac ccggacctgc cggcccgcct cggcgagcag
     1741 ggcggcgacg gccaggccgg cgggcccggc cccgatcacg accacgtcgc tccgcatcag
     1801 ccggtgaccc cgctcccggt ggctgcggcg gctgccgcct cgtcccgctc gccgctcggc
     1861 tccatgtcga agacgccccg gtagatcgcc tggttgagca gggcctggcg cgcctggtcc
     1921 ccccagacgg aggcgacctg cccgcgccag cgctcctcca ggaagcgctc cagcgcggcc
     1981 gaggggtcgg ggaggcggga ctccgcgtcg gcctcggtcg aggcgtactc gcacaggatg
     2041 ccggcagccc gccaggcaca gaacgccgcc tggcacggcc cgtagccgag gcgcagctgg
     2101 cgccgcaggt cctcgagctc gccgaggccc ccggcgaccg ccgaccagac ctggcctctc
     2161 gtcaccagct cgcactcgca gacgagctcg ccgtaggcgc cggcgcgctc gacgctggcc
     2221 agcggtccgg ccagccggtg ggggccgtgg tcggcgccag gcagcacctc ggtggcggtg
     2281 gtgcagccga cggtcacccc catgcgcgca gccagccggt cgacgaggtg ctcggccatc
     2341 tggcggcagg tggtcagctt ccccccgacg atgctgagca tgttctcgag cccgtcccgg
     2401 ctcgcgtggt cgagcaccgt gaaggtccgg ctcaccgccc tggtgtccgc ggcgtccccg
     2461 tccccgccac cggtcgcggg cggcttgtag agcgggcgcg agccggcgaa ggagcgcagc
     2521 acccggccgt gggcgatcga cggcaccatc tgcgcgcaca tgtcgaccat gcggtcgacg
     2581 tcggcggggt cgacggtgat gtcgtccggg tcgggcacct ggaccgaggt cgtgccgagg
     2641 atggcgacct cgtgctgggg cacgatgatg tcgccgtcgg ccggcttgcg gcaggcgttg
     2701 accaccccgc ggacgtaccg gctggccatg acgaccatga cgcccttgcc ggggatcatg
     2761 tggatcggca ccccggccag ctcgccgacg tc
//
LOCUS       DASPYY010000234         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1447747, whole genome shotgun sequence.
ACCESSION   DASPYY010000234 DASPYY010000000
VERSION     DASPYY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447747"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VEP73_03570"
     CDS             complement(<1..810)
                     /locus_tag="VEP73_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYT25548.1"
                     /translation="MTVLCLIETDAAGAADASLRALAVARELAGSAGEDLAACWFGPV
                     AAVDHAELAAAGVSSARAIGSAGLTGYAPLAWARALAGLAAGGSGGGQAPVSAVVAAA
                     TDHGNEVLAHLAALSDLPMAANCVSASLAAAGEFRLVRQRWAGSLLEDAVLEAPLALL
                     TVASDAVPAELEAAGGTAAGEGDPRVDPYEPELADGDLLVRATESADRKSGVSLASAR
                     VVVGGGRGVGGPDGFAVLEELAGLLGGVVGVSRVVTSEGWRPHRQQVGQTGT"
     gene            complement(807..1592)
                     /locus_tag="VEP73_03575"
     CDS             complement(807..1592)
                     /locus_tag="VEP73_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HYT25549.1"
                     /translation="MEICVCVKRVPMVGGAIMVTPDGMDIDVSMSGFTVSPHEECAVE
                     EAVQITERLGGTVTVLTLGPAEAAGQLRDMLALGAGRGVLLQTDGAEWGPVATAAAIA
                     DAVRDGGTSAFELLLFGNEAADTGGYQVPVRVAHALGLPCVTGIKGIEVRGAGAGDPG
                     PPLILASREYRGAEEVFELPLPAVVSVKEGINLPRYPSLPGRLRAKRASIETIEPRQG
                     SDGLRMERLRVPDSSGKAAQVLGAGADAVPALVGLLDELGVLP"
     gene            complement(1594..>2190)
                     /locus_tag="VEP73_03580"
     CDS             complement(1594..>2190)
                     /locus_tag="VEP73_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015320680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HYT25550.1"
                     /translation="YVGELGCELYVPMEEGGKLWDALWDAGQPSGLIPAGIGVYGTTG
                     RLEKGYRAFGNELTGDFNLVEAGMTRPKVKAQDFVGKAAYLAQREQPPAAVLCTLTVD
                     DHASASGELRYPMGGEPVLGPDGAVLTDARGRTSYVTSAGSGPSVGKHILLAYLPAAD
                     AVEGRSLAIEYLGERYPVTVAVAGSTPLFDPANDRIRC"
BASE COUNT          296 a          871 c          765 g          258 t
ORIGIN      
        1 ggtgccggtc tggccgacct gctgccggtg cggccgccag ccctcgctgg tcaccacgcg
       61 ggacacgccg accacgccgc cgagcagccc ggccagctcc tcgaggacgg cgaacccgtc
      121 gggcccgccg acgccgcgcc cgccgcccac cacgacccgc gcgctcgcca gcgacacccc
      181 tgacttcctg tcggccgact cggtggcgcg gacgagcagg tcaccgtcgg ccagctcagg
      241 ctcgtacgga tcgacccgcg ggtcgccctc gccggcggcg gtgccgccgg ccgcctccag
      301 ctcggcggga acggcatcgg atgccaccgt gagcagcgcc agcggcgcct cgagcacggc
      361 gtcctccagc aggctgcccg cccagcgctg ccgcacgagc cggaactcac cggctgccgc
      421 gagcgacgcc gagacgcagt tcgccgccat cggcaggtcg ctgagggcgg cgaggtgggc
      481 gagcacctcg ttgccgtgat cggtggccgc cgcgaccacc gcagaaaccg gtgcctggcc
      541 accgccggag ccgccggcgg ccaggccagc cagcgccctg gcccaggcca gcggcgcgta
      601 cccggtgagc ccggcagacc cgatcgcccg ggccgacgac accccggcgg ccgccagctc
      661 cgcgtgatcg accgcggcaa cagggccgaa ccagcaggcc gcgaggtcct cgcccgccga
      721 gccggccagt tcccgcgcca cggccagcgc gcgcagcgac gcgtcggccg cgcccgccgc
      781 gtcggtctcg atcaggcaca gcaccgtcat ggcagcaccc cgagctcgtc gagcaggccg
      841 accagcgccg gaaccgcgtc ggctcccgcg ccgagcacct gggctgcctt gcccgacgaa
      901 tcgggaaccc gcagccgctc catgcgcagc ccgtcgctcc cctgcctggg ctcgatcgtc
      961 tcgatgctgg cgcgcttggc gcgcagccgg ccggggagcg aagggtagcg cggcaggttg
     1021 atcccctcct tgacgctcac gacggcgggc aggggcagct cgaagacctc ttcggcgccg
     1081 cggtactcgc ggctggcgag gatcaggggc gggccggggt cgcccgcgcc ggcgccgcgt
     1141 acctcgatcc ccttgatccc ggtcacgcag ggcagtccga gcgcgtgcgc gacgcgcacc
     1201 ggcacctggt acccgcccgt gtcggccgcc tcgttgccga acagcagcag ctcgaacgcc
     1261 gaggtcccgc cgtcacggac cgcgtcggcg atcgctgccg ccgtggcgac cgggccccac
     1321 tcggcgccgt cggtctgcag caggacgccg cgcccggcgc ccagggccag catgtcgcgc
     1381 agctggccgg cggcctcggc cggcccgagc gtcagcacgg tgaccgtgcc gccgagccgc
     1441 tcggtgatct gcaccgcctc ctcgacagcg cactcctcgt gcgggctgac ggtgaagccg
     1501 gacatcgaca cgtcgatgtc cataccgtcc ggcgtgacca tgatcgcgcc gccgaccatc
     1561 gggacccgtt tcacgcagac gcagatctcc acgtcagcac ctgatccggt cgttcgccgg
     1621 gtcgaacagc ggcgtggacc cggcgaccgc gaccgtgacc gggtagcgct cgccgagata
     1681 ctcgatggcg agcgaacggc cctcgacggc gtcggcggcc ggcaggtacg cgagcaggat
     1741 gtgcttgccc actgacggcc cggagcccgc actggtgacg tacgaggtcc ggcccctggc
     1801 gtcggtcagc accgcgccat cggggccgag caccggctcg ccgcccatcg gataccgcag
     1861 ctcgcccgag gcggaggcgt ggtcgtccac ggtcagggtg cacagcaccg cggcgggcgg
     1921 ctgctcgcgc tgcgcgaggt aagcagcctt gccgacaaag tcctgcgcct tgaccttcgg
     1981 ccgcgtcatc ccggcctcga cgaggttgaa gtcgccggtc agctcgttgc cgaaggcgcg
     2041 gtagcccttc tcgagccggc ccgtcgttcc gtagacgccg atgcccgccg ggatgaggcc
     2101 ggatggctgg cccgcgtccc agagcgcgtc ccacagcttg ccgccctctt ccatcgggac
     2161 atacagctcg cagccgagct cgccgacgta
//
LOCUS       DASPYY010000235         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1869462, whole genome shotgun sequence.
ACCESSION   DASPYY010000235 DASPYY010000000
VERSION     DASPYY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869462"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VEP73_03585"
     CDS             complement(<1..680)
                     /locus_tag="VEP73_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYT25551.1"
                     /translation="MTTEEANAPAALDRPAPRVAVVGRPNVGKSTLVNRILGRRAAIT
                     EEQPGVTRDRVTYQAEWSGHRFELVDTGGWEPRASGLQARVVAQAERAMAEADLLLFV
                     VDATTGITEEDAAVAARLRKGVVPVLLVANKVDNAATEPQASGFWSLGLGEPFPVSSL
                     HGRGAGDLLDEVVAHLGDAVPSREEPGPPAIAIVGRPNVGKSSLLNRMAGAEVAIVDP
                     TPGTTRDTV"
     gene            complement(695..1396)
                     /locus_tag="VEP73_03590"
     CDS             complement(695..1396)
                     /locus_tag="VEP73_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT25552.1"
                     /translation="MSTTGSYRVYRAAALVVKPSMHVWFRMRIEGAAQVPATGPVILA
                     CNHRSNVDPVLLASAIRRPVTFMAKAELFVGPLRWILNAIEQFPVRRGGIDREALRDC
                     AAVLGRGGVLGLFPEGTRGDGRFTTIHPGVAYVVVQQNCPIVPVAIFGTERVRRRFGW
                     LPWAAPVRIVFGAPFQLPDPEADPGPGDGGARAARRAATDQLGRLLREFLERVERQRR
                     RRDGHDADTDILTNQ"
     gene            complement(1403..2173)
                     /locus_tag="VEP73_03595"
     CDS             complement(1403..2173)
                     /locus_tag="VEP73_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYT25553.1"
                     /translation="MNNDVVVAIDGPAGSGKSTVARAVAARTGLRYLDTGSTYRALTL
                     GLLRAGVPSDDAEAVAAAAPEVRLALQLAETPGAASRVLLDGVELAPEQLRSRAVNDA
                     VSPVSAVSAVREHLVELQRSAMTGGGVVAEGRDVGATVWPEAEVKVFLIASAAERARR
                     RGGGGVSNGANSSSDAGPDRPGPDSGEEGDGESAEAVARRDRLDSSRAASPTRPGEGA
                     VVIDTTDRPVSEIVDQVVQLIEAARTRTHAQAPPGRPR"
     gene            complement(2309..>2690)
                     /locus_tag="VEP73_03600"
     CDS             complement(2309..>2690)
                     /locus_tag="VEP73_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYT25554.1"
                     /translation="VAALDALSERITTLRALVAKGDHEGLRETLAEARSARLRLPTKP
                     GTSPRAMRHLVVHIPDRPGSLAAVFRALGAAGVNVEDLSVDHELQGGSGDLHVWVAGR
                     DALSTAQAALSSAGWTAYETADNP"
BASE COUNT          336 a          943 c         1024 g          387 t
ORIGIN      
        1 acggtgtcgc gggtggtgcc gggggtcggg tcgacgatcg cgacctcggc gccggccatg
       61 cggttgagca ggctggactt gccgacgttg ggccggccga cgatggcgat cgccggcggg
      121 ccaggctcct ccctgcttgg caccgcgtcg ccgaggtggg cgacgacctc gtcgaggagg
      181 tcgccggcgc cgcggccgtg caggctcgac accggaaacg gctcgccgag gccgagcgac
      241 cagaagccgc tggcctgcgg ttcggtagcg gcgttgtcga ccttgttggc gaccagcagg
      301 accggcacca cgcctttgcg caggcgcgca gcgacggcgg cgtcctcctc ggtaatgccg
      361 gtggtggcgt cgaccacgaa cagcagcagg tcggcctcgg ccatggcccg ctcggcctgg
      421 gccacgacgc gggcctgcag gccgctggcg cggggctccc agccgccggt gtcgaccagc
      481 tcgaagcggt ggccgctcca ctcggcctgg taggtgaccc ggtctctggt gacgccgggc
      541 tgctcctcgg taatcgcggc gcgccggccg aggatgcggt tgaccagggt cgatttgccg
      601 acgttgggcc gcccgaccac cgcgacccgt ggtgccgggc ggtccagcgc cgctggggcg
      661 ttcgcctctt ctgtcgtcat agcagcttat cgattcattg atttgtcaat atatcggtat
      721 cagcgtcatg cccgtcccgc cgtcgccgct gccgctcgac gcgctccagg aactcccgca
      781 ggaggcggcc gagctggtcg gtggcggcac gccgggcggc gcgggcacca ccgtccccgg
      841 ggccggggtc ggcctcggga tcggggagct ggaacggcgc accgaacacg atgcgcaccg
      901 gcgcggccca cggcagccag ccgaagcggc gccggacccg ctcggtgccg aagatcgcca
      961 ccggcacgat cgggcagttc tgctgcacga ccacgtaggc gacgccgggg tggatggtgg
     1021 tgaagcggcc gtcgccgcgg gtgccttcgg ggaacaggcc gagcaccccc ccgcgcccaa
     1081 gcacggcggc gcagtcccgc agcgcctccc ggtcgatgcc gccgcgccgc accgggaact
     1141 gctcgatggc gttgaggatc cagcgcagcg gccccacgaa cagctccgcc ttggccatga
     1201 aggtgacggg gcggcggatg gcggaggcga gcaggaccgg gtccacgttg ctgcggtggt
     1261 tgcaggcgag gatcaccggg ccggtggcgg ggacctgtgc ggcgccctcg atgcgcatgc
     1321 ggaaccagac gtgcatcgag ggtttcacca ccagggcggc ggcccggtag acgcggtagg
     1381 agcctgtggt gctcatctca tctcaccggg gacggccggg cggggcctgg gcgtgggtgc
     1441 gggtcctggc cgcctcgatc agctggacga cctggtccac gatctcgctg acgggcctgt
     1501 cggtggtgtc gatgaccacg gcgccctcgc cgggccgggt tggtgaggcg gcgcggctgg
     1561 agtccaggcg gtcgcggcgg gccaccgcct cggcggactc gccatccccc tcctcgccgc
     1621 tgtccgggcc gggtcggtcc gggccggcgt cgctgctgct gttcgccccg ttggagacac
     1681 cgccaccgcc acggcggcga gcccgctcgg cggccgaggc gatgaggaag accttgacct
     1741 cggcctcggg ccagacggtg gcgcccacgt cgcgcccttc ggcgacgaca ccgcctcccg
     1801 tcatggccga gcgctgcagc tccaccaggt gctcgcgcac cgccgagacc gccgagaccg
     1861 gcgagacggc gtcgttgacc gcccgggagc gcaactgctc gggggcgagt tcaaccccgt
     1921 ccagcagcac ccgcgaggcc gccccgggcg tctccgcaag ctgcagggcg agccggacct
     1981 cgggcgcggc ggcggcgacc gcctcggcgt cgtcgctggg gacgcccgcc cgcagcagcc
     2041 cgagggtgag cgcccggtag gtcgagccgg tgtccaggta gcggagcccc gtccgcgcgg
     2101 cgaccgcacg ggcgacggtg ctcttgcccg acccggcagg gccgtcgatc gcgaccacga
     2161 cgtcgttgtt cacggctgta cgcctgcctc ctgcctggat ttctgggtct tgtgggccgc
     2221 atgagtctac tcatgggcct gtaacgccag catgtattga cttggtgatc cgtcgatgga
     2281 tcgataaatc tatatatcaa tgtatcggtt aggggttgtc cgcggtctcg taggcggtcc
     2341 agccggcgct gctgagggct gcctgggcgg tgctgagggc gtcgcggccg gcgacccaga
     2401 cgtgcaggtc gccgctgccg ccctgcagct cgtggtcgac gctgaggtcc tcgacgttga
     2461 cgccggcggc gccgagcgcg cggaagaccg cggccaggct gcccggccgg tcggggatgt
     2521 ggaccaccag gtgccgcatg gctcgtggcg aggtgccggg cttggtcggc aggcgcagcc
     2581 gcgcgctgcg cgcctcagcc agcgtctccc gcaggccctc atggtcgccc ttggcgacca
     2641 gcgcccgcag ggtggtgatg cgctcactga gggcgtccag cgcggcgacg
//
LOCUS       DASPYY010000236         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1026975, whole genome shotgun sequence.
ACCESSION   DASPYY010000236 DASPYY010000000
VERSION     DASPYY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026975"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..794
                     /locus_tag="VEP73_03605"
                     /pseudo
     CDS             144..794
                     /locus_tag="VEP73_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            complement(859..1488)
                     /locus_tag="VEP73_03610"
     CDS             complement(859..1488)
                     /locus_tag="VEP73_03610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HYT25555.1"
                     /translation="MRVLLIGPPGAGKGTQATRIADHFDLARIATGDLLREEVAAGTE
                     LGTVAKQYMDLGDLVPDDVVIAMTRDRVVRANTEGGYILDGYPRTLAQAEAAYRWAAA
                     RGVPFDLALFFEIGEDELLARLAGRAREQGRSDDNEATVRHRLDVFAARTRPLLEYYE
                     RRGIVVRVDAVGPLDAITERILAALHWHKANALAAGADRPEWASGRGTG"
BASE COUNT          245 a          713 c          667 g          232 t
ORIGIN      
        1 cgcgccgccg ggttcccgac ctgggagcag accgcccggc ggttcttcac ggcgatccgc
       61 acggcggccc tcggcccgat gcggggcaac caccgtccca ggcggccgcg aacagcggag
      121 gacgcagagt gaccagcggg atcgaccgca cgccagggtg gatggcacca gccgacccgc
      181 ccaccacgac cgtgctgctg cgccacggcg acacgccgct ctcgcgcgag aagcgcttca
      241 gcggcgttgg cgacgcaaga ctgaccagga cgggcgcggc gcaggcgcac gcagccgccg
      301 cgcggctgcg tgcacgcggc ggcatcgggg cggtcgtgtc ctccccgctg cgccgggccc
      361 tgcagaccgc cgaggtcgca gccgccgccc tggacctgcc ggtgctggtc gacgaggacc
      421 tgcgtgagac cgacttcggc gcatgggagg ggcgcacctt tgccgaggcg ctggtgcgct
      481 ggcccgacct ggtggctgcg tggctggccg acccgtcggt ggcgcccccc ggcggggaga
      541 gcttcgccga caccacgatc cgggtccagc acgcacgcga ccggctcctt gcgcgcatgc
      601 ctcgcgggac catcctggtc gtcagccacg tgacgccgat caagacgctg gtccgtctgg
      661 ccctccaggc gccgccggtc gcgctgcacc gtatgcacct tgactccgca tgcctgtcgg
      721 aactggactg gtaccaggac ggccccgcgg tccttcgcgc cttcaacgac acccaccacc
      781 tgccacccga ctgatggccc aactggccgc tgaccgaccg cgagctgcag gtgccacggt
      841 catccgtgca ggccagcgct agccggtgcc ccggccggac gcccactcgg gccggtcggc
      901 gcccgcggcc agcgcgttgg ccttgtgcca gtgcagcgcg gccaggatcc gctcggtgat
      961 cgcgtcgagc ggcccgaccg cgtcgacccg gaccacgatc ccgcgccgct cgtagtactc
     1021 gagcagtggg cgggtccgcg ccgcgaacac gtccaggcgg tggcggaccg tcgcctcgtt
     1081 gtcgtcgctg cggccctgct cccgcgcccg gccggccagc cgggcgagca gctcgtcctc
     1141 gccgatctcg aagaacaggg ccaggtcgaa cggcaccccg cgggccgccg cccaccggta
     1201 ggcggcctcg gcctgggcga gcgtacgcgg gtagccgtcg aggatatagc cgccctcggt
     1261 gttcgccctg accacccggt ccctggtcat cgcgatcacg acgtcgtcgg ggacgaggtc
     1321 gccgaggtcc atgtactgct tcgcgacggt gccgagctcc gtccccgcgg cgacctcctc
     1381 ccggagcagg tcgccggtcg cgatcctggc caggtcgaaa tggtcggcga tccgggtggc
     1441 ctgggtgccc ttgcccgcgc ccggcggtcc gatcagcagc acacgcatcc ccgctcctcc
     1501 tcgtccaggc gacgtacacg gcggcggcga cgtcccagcc gatgagcatg ccgagctgcc
     1561 aggtccccgg cagcgacgcc agcgtgccca cgcaggtgcc ggtggccgcg gccgtggcga
     1621 cgccggaagg atgcccggtc agggccaaac aatagccatc cgccggccag gggcgccgcg
     1681 gggcgacggc acgccgcgcg ccgccggtcg gcccatggcc ctcacgacgg gccggggctg
     1741 ggcgcggctc gcttgtcggc gccgcgcacg gtgtcgagca cggtgtgggc ctgctggtcg
     1801 acgaagcggg tgaacagctg ctgcgcgtac ccgaacacga gggcccaggc gagaatc
//
LOCUS       DASPYY010000237         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2571075, whole genome shotgun sequence.
ACCESSION   DASPYY010000237 DASPYY010000000
VERSION     DASPYY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571075"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /gene="purL"
                     /locus_tag="VEP73_03615"
     CDS             complement(<1..1590)
                     /gene="purL"
                     /locus_tag="VEP73_03615"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HYT25556.1"
                     /translation="MSSVAPPLHVQLGLTDDEYDRILAVLGREPHRAELAMYSLMWSE
                     HCSYKSSKVHLRGLPTEGPHVLQGPGENAGVVDVGDGLAAVFKIESHNHPSFVEPFQG
                     AATGVGGIIRDILAMGARPLASLDSLRFGDLAEPRQRRLLEGVVAGIGHYGNCVGVAT
                     VGGEVAFDPCYAGNPLVNALTVGIMPADRLVLGRAERPGHLAVLMGAKTGRDGIGGAS
                     VLASSSLDPGSQAKRPNVQVGDPFQEKLLIEACLELVDRGLLSGLQDLGAAGISCAIS
                     EVAARAGMGMDVDLDAVPLREPSMQAWEVLASESQERMLALVAPDDLEAVLEVCRRWG
                     ILASVIGRITGGASPVSGGAGRLTVRFRGELVADVPARSLVDDGPLYHRPLTPPAPPE
                     ADAVVPLDADPAETVLALAGDPTCASKRWVYEQYDRFVGHGTLAGPGSDAAVIRVPGT
                     GRALAVATDGNGRYAGLDPAAGAALAVAEAARNVACTGARPLAATNCLNFASPERPEV
                     MGAFAAAVAGMAAACLALGTPV"
BASE COUNT          234 a          657 c          673 g          217 t
ORIGIN      
        1 gaccggggtg ccgagcgcca ggcaggcggc tgccatgccg gcgacggccg ccgcgaaggc
       61 gcccatgacc tcggggcgct ccgggctcgc gaagttgagg cagttggtcg cggcgagcgg
      121 ccgcgcgccg gtgcacgcca cgttgcgggc cgcctcggcg acggccaggg ccgccccggc
      181 ggccgggtcc agcccggcat agcggccgtt cccgtcggtg gccacggcga gcgcccgtcc
      241 ggtgcccggg acgcggatca cggcggcgtc cgagcccggc ccggccaggg tgccatggcc
      301 gacgaaccgg tcgtactgct cgtacaccca ccgcttggac gcgcacgtcg ggtccccggc
      361 cagggcgagg accgtctcgg ccgggtccgc gtcgaggggc accacggcgt cggcctcggg
      421 cggtgccggc ggggtcagcg gccggtggta gaggggcccg tcgtcgacca ggctgcgggc
      481 gggcacgtcc gccaccagct ccccccggaa ccggacggtg agcctgccgg caccgcccga
      541 gacgggcgag gccccgccgg tgatgcggcc gatcacgctc gcgaggatgc cccagcggcg
      601 gcacacctcc agcaccgcct ccaggtcgtc cggggcgacc agggcgagca tgcgctcctg
      661 cgactcggac gcgagcacct cccaggcctg catcgacggc tcgcgcagcg gcacggcgtc
      721 gaggtcgacg tccatcccca tgcccgcccg cgcggccacc tccgagatgg cgcaggagat
      781 gccggccgcc cccaggtcct gcaggccgct gagcagcccc cggtcgacca gctcgaggca
      841 ggcctcgatg agcagcttct cctggaaggg gtcgccgacc tggacgttcg gccgcttggc
      901 ctggctgccc gggtccaagc tggaggaggc gagcacgctc gcgccgccga tcccgtcgcg
      961 gcccgtcttg gctcccatca ggacggcgag gtggcccggc cgctcggccc ggccgagcac
     1021 cagccggtcg gccggcatga tgccgaccgt gagcgcgttg accagcgggt tgccggcgta
     1081 gcaggggtcg aaggcgacct cgccccccac ggtggccacc ccgacgcagt tgccgtagtg
     1141 gccgatgccg gccacgaccc cctcgagcag tcgacgctgc cgcggctcgg ccaggtcccc
     1201 gaagcggagc gagtcgaggc tggccagggg acgcgcgccc atcgcaagga tgtcgcggat
     1261 gatgccgccc acgccggtcg cggcgccctg gaacggctcg acgaacgacg ggtggttgtg
     1321 cgactcgatc ttgaacacgg cggccagccc gtcgcccacg tcgacgacgc ccgcgttctc
     1381 gcccggcccc tgcaggacgt gaggcccttc ggtgggcagg ccgcgcaggt ggacctttga
     1441 ggacttgtag gagcagtgct ccgaccacat cagcgagtac atcgcgagct cggcccggtg
     1501 gggctcacgc ccgagcaccg cgaggatgcg gtcgtactcg tcgtcggtga gcccgagctg
     1561 cacgtgcagt ggcggcgcga cgctggacac gctcactccc cagctggatc gtccggaagc
     1621 cttgtggggc cgaggctacc atcgcgcggg cgcgctagaa gctgaagcag agcgcggagt
     1681 ccaggagctt ccggcaggtc accgccgtcg cggcggtgcc ggtctggccg cggtcgtcgg
     1741 tcaccgtgag cgtgacggtg tagtcggcgt ccttggacgg a
//
LOCUS       DASPYY010000238         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_465321, whole genome shotgun sequence.
ACCESSION   DASPYY010000238 DASPYY010000000
VERSION     DASPYY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465321"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VEP73_03620"
     CDS             <1..1269
                     /locus_tag="VEP73_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT25557.1"
                     /translation="FFGQMAKWVGQAEDPARLPELLARAFAAAASGRPGPVVLGLPED
                     VLTGKVEVADAAPYRVVEPSPDPAALARLRGLLAGARRPLVLAGGAGWTAAAAADLRA
                     FAEASQLPVAASWRSQDVIDNRSPSYVGHVGLGIDPSLARRVRETDLLVAVGPRLGEI
                     TTGGYTLLEVPRPRQALVHVHPAVEELGRVYQADLPVHAGMGPFAAAARALPPVSGPP
                     WRAWTGAARRAWLAWSRPPPGGAALDLARVVAWLRERLPDDAVVANGAGSFSAWLHRF
                     WTYTATGTQLGPRGGAMGYGLPAAVAAKLHSPERPVLAFTGDGDFLMTGQELATAVQL
                     GLPVVVVLVNNSMYGTIRMHQERAYPGRVVGTDLVNPDFAALARAFGAHGEAVTRTEE
                     LAPAFERAVASGRPAVLELRVGVESLAPPS"
BASE COUNT          171 a          556 c          663 g          186 t
ORIGIN      
        1 ttcttcggcc agatggcgaa gtgggtgggc caggcggagg atccggcgcg cctgcccgag
       61 ctgctcgccc gcgcgttcgc ggcggcggcg tccggccgcc cgggcccggt cgtgctcggc
      121 ctgcccgaag acgtgctgac cgggaaggtc gaggtggccg acgcggcccc gtaccgggtg
      181 gtggagccgt cccccgaccc ggcggccctg gcacggctgc gggggctgct ggccggggcg
      241 cggcggccgc tggtgctggc cggcggggcc gggtggacgg ccgcggcagc ggccgacctg
      301 cgggcgttcg cggaggcgag ccagctgccg gtggcggcgt cgtggcggtc gcaggacgtg
      361 atcgacaacc ggtcgccgag ctacgtgggg cacgtggggc tcggcatcga cccgtcgctc
      421 gcccggcgcg tgcgggagac ggacctgctg gtcgcggtcg ggccccggct gggcgagatc
      481 acgacgggcg ggtacacgct gctcgaggtg ccgcggccgc ggcaggcgct cgtccacgtg
      541 catccggcag tcgaggagct gggccgggtg taccaggccg acctgccggt ccacgccggg
      601 atgggcccgt tcgcggcggc ggcccgggcg ctgccgcccg tcagcggccc gccctggcgg
      661 gcgtggaccg gagcggcgag gcgggcatgg ctggcatgga gccgcccgcc gcccggcggg
      721 gccgcccttg acctcgcccg ggtggtggca tggctgcggg agcgcctgcc cgacgacgcg
      781 gtggtggcga acggggcggg gagcttctcg gcgtggctgc accggttctg gacctacacg
      841 gcgacgggga cccagctcgg cccgcggggc ggcgcgatgg ggtacggcct gccggcggcg
      901 gtggcggcga agctccactc ccccgagcgg ccggtgctcg cgttcaccgg cgacggggac
      961 ttcctgatga ccgggcagga gctggcgacc gcagtgcagc tcgggctgcc ggtcgtggtc
     1021 gtgctggtga acaactcgat gtacgggacg atccggatgc accaggagcg ggcctacccg
     1081 gggcgggtcg tgggcacgga cctcgtgaac ccggacttcg cggcgctggc gcgtgcattc
     1141 ggggcgcacg gcgaggcggt gacccggacc gaggagctcg cgccggcgtt cgagcgggcg
     1201 gtggcctcgg gccgcccggc cgtgctcgag ctccgggtgg gcgtggaatc gctggccccg
     1261 ccctcgtagg cgacggcgtc ggtcatcgcc acccccgggg cgccgggctc ccggccctcc
     1321 cgtcgccggc gaagcagaac acgtcgtagg cacggccgat caccggctgc gccacgttgc
     1381 ggaccgggac gccgccctcg ggccgtcagc ggctgccgcg tcccgctctc gctacccgag
     1441 cgggcgcggg tgaaaccgac gcgcccgcgg accgggccgg cggattaccg ggaccagggc
     1501 cgtggtagcc cgggtgtggg cccaccggtc aacagccacc agaccacagg agggacagcc
     1561 atgggcagcc agcacg
//
LOCUS       DASPYY010000239         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1588521, whole genome shotgun sequence.
ACCESSION   DASPYY010000239 DASPYY010000000
VERSION     DASPYY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588521"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VEP73_03625"
     CDS             <1..1277
                     /locus_tag="VEP73_03625"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT25558.1"
                     /translation="HCVYTNTSPSGSYRAFGASHLQWIAESQLDEVARRLGIDRLEIR
                     RQNLLRPGEEVRPGGKPLDADLVGDVEKAAAAIGWSGGSGGDDSPPGSGGSRGVVPPG
                     RYRRGRGVSVGLLAAGAHPVSMATVRMGPDGSVTVLNGTTELGQGARTVMAQIAAGVL
                     RAPVGKVVVRGTDTRFTPYDRSTGASRSTTVAGLAVQRAAQDVLNSLLETAAAKLEAP
                     PQLLTPVEGGIRDAGGRTLTHAELVRARFGFDGGELLGHGRVQPEGGTGSYAEGPVFW
                     EVCVAGAEVEVDLETGVVTVLRTSTVADVGKAINPQLVTRQDEGATVAGLGNALFEEM
                     HFAPDGALLNDTLLGYRIPAFRDIPATMTCSIVENGDGPGPFGAKGCGEGALAAVPAA
                     IVNALADAGVPMNTLPLTPERVWRRIRELKIS"
     gene            1295..>1618
                     /locus_tag="VEP73_03630"
     CDS             1295..>1618
                     /locus_tag="VEP73_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYT25559.1"
                     /translation="MSVPKVAAVIGAGTMGPGMSAVLARAGSEVRLYDISDEVLERAK
                     AGCQLAAGALDRVEAPAAPGGSVAFEADLGRALAGAELVLEAIPEKLELKREVTAQLE
                     EHLTDD"
BASE COUNT          219 a          581 c          614 g          204 t
ORIGIN      
        1 cgcactgcgt ctacaccaac acgagcccat ccgggtccta ccgcgccttc ggcgccagcc
       61 acctgcagtg gatcgctgag tcgcagctgg acgaggtggc ccgccggctg ggcatcgacc
      121 ggctggagat ccgccgccag aacctgctcc ggcccgggga ggaggtccgc cccggcggca
      181 agccgctcga cgccgacctc gtcggcgacg tggagaaggc ggctgccgcc atcggctggt
      241 cgggggggtc tgggggggac gattctcccc caggctcggg gggatcccgg ggggtcgtcc
      301 ccccggggcg gtaccgtcgc ggccgtggcg tgtccgtggg cctgctcgcg gccggggccc
      361 accccgtgtc gatggcgacg gtccggatgg gcccggacgg gtcggtcacc gtcctgaacg
      421 gcaccaccga gctcggccag ggggcccgaa cggtgatggc gcagatcgcg gccggcgtgc
      481 tgcgagcccc cgtcggcaag gtcgtggtgc gcggcaccga caccaggttc acgccgtacg
      541 accggtccac cggggccagc cgctccacca cggtggccgg cctggccgtg cagcgggccg
      601 cgcaggacgt gctcaactcc ctgctcgaga ccgccgccgc gaagctggaa gccccgcccc
      661 agctgctcac gccggtcgaa ggcgggatcc gggacgcggg cggcaggacc ctgacccacg
      721 ccgagctcgt ccgggcccgc ttcggcttcg acggcggcga gctcctcggc cacggccggg
      781 tccagcccga gggcggcacc ggctcctacg ccgaggggcc ggtcttctgg gaggtgtgcg
      841 tggccggggc cgaggtggag gtggacctcg agaccggcgt cgtcaccgtg ctgcgcacct
      901 ccaccgtggc cgacgtgggc aaggccatca acccgcagct ggtcacccgg caggacgagg
      961 gagccaccgt ggcgggcctc ggcaacgccc tgttcgagga gatgcacttc gcccccgacg
     1021 gcgccctgct gaacgacacg ctgctcggct accggatccc cgctttccgt gacataccgg
     1081 ccaccatgac gtgctcgatc gtcgagaatg gcgatggccc cgggccgttc ggggccaagg
     1141 gctgcggcga gggtgcgctg gccgcggtcc cggccgcgat cgtcaacgcg ctggccgacg
     1201 ccggcgtgcc gatgaacacg ctgccgctca ccccggaacg ggtgtggcgg cggatacgag
     1261 aactgaagat cagctgaaac ccggaggtta aggcatgagc gttccgaagg tcgcggcggt
     1321 gatcggcgcc ggcacgatgg gcccgggtat gtcggcggtg ctggcccgcg ccggttccga
     1381 ggtacggctg tacgacatca gcgacgaggt gctcgaacgg gccaaggccg gctgccagct
     1441 ggcggccggc gccctggacc gggtggaggc ccccgccgcc ccgggcggtt ccgtcgcctt
     1501 cgaggccgac ctgggccggg cgctggccgg cgccgagctg gtccttgagg ccatcccgga
     1561 gaagctggag ctcaagcgcg aggtcaccgc gcagctcgag gagcacctga ccgacgac
//
LOCUS       DASPYY010000240         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2009699, whole genome shotgun sequence.
ACCESSION   DASPYY010000240 DASPYY010000000
VERSION     DASPYY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009699"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="glgP"
                     /locus_tag="VEP73_03635"
     CDS             complement(<1..869)
                     /gene="glgP"
                     /locus_tag="VEP73_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYT25560.1"
                     /translation="MRPLRRFTVLPAVPSALEPLRALARNLRWSWDPLTQDLFADIDP
                     SLWESSGHNPLRMLNESDPRRLEWAATDPDFLARLARAKGALDRYLTEPRWYQSLTGA
                     PFGIAYFSPEFGISEVLPQYSGGLGVLAGDHLKAASDLGLPLVGVGLFYRSGYFRQSL
                     TADGRQQESYPAFNPHELPFELVTDDAGGRLLVRVRLPDATLHAQIWRAEVGRVSVLL
                     LDSDVDENAPAERAVTDRLYGGGGEHRLRQEILLGIGGVRALDALGIEPEVFHTNEGH
                     AGFLGLERIRRLI"
     gene            1099..>2781
                     /locus_tag="VEP73_03640"
     CDS             1099..>2781
                     /locus_tag="VEP73_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HYT25561.1"
                     /translation="MIGRIVIEGIRPRVDCGRWPARAVAGETIPVEATVFRDGHDIIA
                     AAVRLSGPGEAGRGPLVPMAEVGQGVDRWRAEVTLPRTGSWTIVIEAWADEIATWRRD
                     TWKKLDAGREVTLELEEGARLFEAAAREVPASSRRLVAGVAEGLRNEAADPAERLAAA
                     SDGTVLDLLERHPMRSVTRSEPLEVWVDRPRALYGSWYEFFPRSEGATETSGGTFKTA
                     AERLPAIAAMGFDVAYLPPVHPIGRTHRKGRDNTLDPGPDDPGSPWAIGGEAGGHDAI
                     HPDLGTIEDFDAFVARARELGLEVALDYALQCSPDHPWVREHPEWFHIRPDGSIAYAE
                     NPPKVYQDIYPLNFDNPDAEGLYAACKGVLEHWIAHGVRIFRVDNPHTKPVRFWERLI
                     AAVKAEHPDVLFLSEAFTRPAMMRMLAKVGFTQSYTYFTWRNSKQELEEYGNELARGP
                     ASEYMRPNFFVNTPDILHAYLQHGGPAAFKVRAVLAATMSPSWGVYSGYELFEHEPAR
                     PGSEEYLHSEKYQYRPRDWSQPSLAPYLTRLNEIRRAHPALHWLRNLHFHGAD"
BASE COUNT          405 a          999 c         1004 g          373 t
ORIGIN      
        1 tcgatcagcc ggcggatccg ttcgagcccg aggaacccgg cgtggccctc gttggtgtgg
       61 aacacctcgg gctcgatccc gagcgcgtcc agcgcgcgga ccccgccgat cccgagcagg
      121 atctcctggc gcaggcggtg ctccccgccg ccgccgtaga gccggtcggt caccgcccgc
      181 tcggccgggg cgttctcgtc gacgtcggag tcgagcagca gcaccgacac ccgcccgacc
      241 tccgcccgcc agatctgggc gtgcagggtc gcgtcgggca gccggacgcg gaccagcagc
      301 cgcccgcccg cgtcgtcggt gaccagctcg aacggcagct cgtgagggtt gaacgccggg
      361 taggactcct gctggcggcc gtcggcggtc aacgactgcc tgaagtaccc cgaccggtag
      421 aacaggccga ccccgaccag cggcaggccg aggtcgctcg ccgccttcag gtggtcgccg
      481 gcgagcaccc cgaggccccc ggagtactgg ggcagcacct cgctgatccc gaactcgggc
      541 gagaagtacg cgatgccgaa cggcgcgccg gtcaggctct ggtaccagcg cggctcggtc
      601 aggtaccggt cgagcgcgcc cttcgcgcgg gcgagccggg cgaggaagtc ggggtcggtg
      661 gccgcccact cgagccgccg cgggtcggac tcgttcagca tccgcagcgg gttgtgcccg
      721 ctggactccc acagggaggg gtcgatgtcg gcgaacaggt cctgcgtcaa cgggtcccac
      781 gaccagcgca ggttgcgggc cagggcacgc aggggctcga gcgcggaggg cacggcggga
      841 agcaccgtga atcggcgaag aggtctcatg agcatcgact atacaagtgc acccgtcggt
      901 cgagccgcgc gacccggtcg gcccctctag gttggtcaca tttgtcgagt tccctcggac
      961 gaggaccgta gtcaggcggg cacgggcggc gctccggccg gtcgacgggt ttaccggtgc
     1021 acgtgggacg gtgaaactgg cagggacaac ggcggtcgag cggacttcgg catggtccgg
     1081 gatggtaggt tgctgggcat gattgggcgc atcgtcatcg agggcatccg gccgcgggtc
     1141 gactgcggtc gctggccggc ccgggcggtg gccggcgaga ccatcccggt cgaggcgacg
     1201 gtcttccggg acggccacga catcatcgcg gccgccgtgc ggctgtcggg gcccggcgag
     1261 gccgggcggg gtccgctcgt gccgatggcc gaggtcggcc agggcgtcga ccggtggcgg
     1321 gccgaggtga cgctgccccg gaccggctca tggacgatcg tgatcgaggc gtgggcggac
     1381 gagatcgcca cctggcgccg cgacacctgg aagaagctgg acgccggccg cgaggtcacc
     1441 ctcgagctcg aggagggcgc gcgcctgttc gaggcggcgg cgcgggaggt gccggcgtcg
     1501 tccaggcggc tcgtcgccgg cgttgcggag gggctgcgca acgaggccgc cgacccggcc
     1561 gagcggctcg ccgcggccag cgacgggacg gtgctcgacc tcctcgagcg gcacccgatg
     1621 cgctcggtga caaggtccga gccgctcgag gtctgggtcg accgcccgcg tgccctctac
     1681 ggctcctggt acgagttctt cccccggtcg gagggggcga cggagacctc gggcggcacc
     1741 ttcaagaccg cggccgagcg gctgcccgcc atcgccgcca tgggcttcga cgtcgcctac
     1801 ctgccgccgg tccaccccat cggccgcacc caccgcaagg gccgcgacaa caccctcgac
     1861 cccggcccgg acgaccctgg cagcccgtgg gcgatcggcg gcgaggccgg cggccacgac
     1921 gccatccatc ccgacctcgg gaccatcgag gacttcgacg cgttcgtggc ccgggcgaga
     1981 gagctcgggc tcgaggtcgc gctcgactac gcgctgcagt gctcccccga ccatccctgg
     2041 gtgcgggagc accccgagtg gttccacatc cggccggacg gctcgatcgc ctacgccgag
     2101 aacccgccaa aggtctacca ggacatctac ccgctgaact tcgacaaccc ggatgcggag
     2161 gggctctacg ccgcctgcaa gggcgtgctg gagcactgga tcgcccacgg cgtgcgcatc
     2221 ttccgggtcg acaacccgca caccaagccg gtgcggttct gggagcggct gatcgcggcg
     2281 gtcaaggccg agcacccgga cgtgctgttc ctctccgagg cgttcacccg cccggcgatg
     2341 atgcggatgc tcgccaaggt cggcttcacc cagtcctaca cgtatttcac ctggcggaac
     2401 tccaagcagg agctcgagga gtacggcaac gagctcgccc gcggccccgc ctccgagtac
     2461 atgcggccca acttcttcgt caacaccccc gacatcctgc acgcctacct gcagcacggc
     2521 gggccggcgg cgttcaaggt gcgggcggtg ctggccgcga cgatgtcgcc gtcctggggc
     2581 gtgtactccg gctacgagct gttcgagcac gagccggccc gtcccgggtc cgaggagtac
     2641 ctccactccg agaagtacca gtaccggccg cgggactggt cgcagccctc gctcgcgccc
     2701 tacctgacgc gcctgaacga gatccggcgc gcccacccgg cgctgcactg gctgcgcaac
     2761 ctgcacttcc acggcgccga c
//
LOCUS       DASPYY010000241         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_745514, whole genome shotgun sequence.
ACCESSION   DASPYY010000241 DASPYY010000000
VERSION     DASPYY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745514"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..324
                     /locus_tag="VEP73_03645"
     CDS             100..324
                     /locus_tag="VEP73_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HYT25562.1"
                     /translation="MDEVTKLAAATGSRDPDVGLAAVAALRALVEVLEALQVGNARAK
                     GWTWQDIAGRLGVSKQAVHKKYAGGRLFRS"
     gene            351..1334
                     /locus_tag="VEP73_03650"
     CDS             351..1334
                     /locus_tag="VEP73_03650"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYT25563.1"
                     /translation="MAQAESTRMVTPEAVALEFRTANLGSRVLAFFLDGLILGAGVLL
                     LLVALVLVGNGTGSDLPGWVAVTLVSLLLPSWLLGYFIAFETLWRGRTPGKAALGLRV
                     VTREGAPVRFRHAAVRALLGLVDYYLGFGFFAVVFILFSRSNQRLGDLVAGTLVLRER
                     SGLAAPAPVSFAPPPGYEAYAGTLDVTRLTTQEYQALRGFLLRAPSLLPVPRVALAQQ
                     LADPLVAHVRPAPPPGVSAEAFLRCVAAAYQHRQRAGRALPGPGWAAPSDPGSTVPLD
                     PARTAPPDPARTAPPDRGGSGRPGPDEPPGPSADQPDEEAGPAAGGFVPPG"
     gene            1423..1968
                     /locus_tag="VEP73_03655"
     CDS             1423..1968
                     /locus_tag="VEP73_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25564.1"
                     /translation="MARREDRSMGYDLTALIGAPDTLRPAVAAVPGARVVDIAAGLAL
                     VPLTGPVLGAIRAGGGERAVAPARGFQALTPAVARFAAGASAAGPVLYAEAEFFGGSG
                     SQAVIGWRGGRVSIGPLLTQTMGEGREGFAVAAGPGLRGMALNAGLRDLGVRTPADGE
                     PWQDEFQAAGLGRCRATEDWS"
     gene            complement(1992..>2685)
                     /locus_tag="VEP73_03660"
     CDS             complement(1992..>2685)
                     /locus_tag="VEP73_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYT25565.1"
                     /translation="GTGQSTIDGILRATNLLLAGTTFVVAGYGYCGKGLAMRARGLGA
                     KVVVTEVDPVRALEAAMEGYRVAPMAEAAREADVIVTVTGDRDILRREHFQVMRDGVV
                     LANSGHFDVEIDKVALRELATSVTRVRENVEEFQAADGRRINLVAEGRLVNLGAAEGH
                     PAAVMDMSFADQALSLEWLARQDTLEVRVHDVPAEIDAEVARVKLATMGIEIDTLTDT
                     QVEYLHSWTEGT"
BASE COUNT          305 a          991 c         1021 g          368 t
ORIGIN      
        1 tagcgatcga cgtccacccg gggatcttaa aggagcttgg gatcgggttg acttcgcctg
       61 ccgagtcaac gtaagttgac tggcatgagc gaggcgcggg tggacgaggt gacgaagttg
      121 gcggcggcga ccggcagcag ggaccccgac gtggggctgg cggccgtcgc tgccctgcgg
      181 gcgctggtgg aggtgctcga ggccctgcag gtcggcaacg cccgggccaa ggggtggacc
      241 tggcaggaca tcgccggcag gctcggggtc agcaagcagg cggtgcacaa gaagtacgcc
      301 ggcggcaggc tgttccggtc atagacccgc tcgaagggat tccgtgagag atggcgcagg
      361 ccgagtccac ccgcatggtg accccggagg cggtcgccct cgagttccgc accgccaacc
      421 tcggctcgcg ggtcctggcc ttcttcctcg acgggctcat cctcggcgcc ggcgtgctcc
      481 tgctgctggt cgcgctcgtc ctggtcggca acggcaccgg ctcggacctg cccgggtggg
      541 tcgcggtcac cctggtgtcc ctgctcctgc cgagctggct cctcggctac ttcatcgcct
      601 tcgagacgct gtggcggggg cggacgcctg gcaaggccgc gctcgggctg cgggtcgtga
      661 cccgcgaggg agcgccggtc aggttcaggc acgcggcggt ccgcgccctg ctcggcctgg
      721 tggactacta cctcgggttc gggttcttcg ccgttgtctt catcctgttc agccggagca
      781 accagcggct cggcgacctg gtcgccggca cgctggtgct gcgcgagcgc agcggcctcg
      841 ccgccccggc cccggtgagc ttcgcgccgc cgcccggcta cgaggcctac gcgggcacgc
      901 tcgacgtgac ccggctcacc acccaggagt accaggcgct gcgcggcttc ctgctgcgcg
      961 cgcccagcct gctccccgtg ccgagggtcg cgctcgccca gcagctcgcc gacccgctcg
     1021 tggcccacgt acgcccggca ccaccgcccg gggtgtccgc cgaggcgttc ctgcgctgcg
     1081 tggcagccgc ctaccagcac cgccagcgag cgggtagggc cctgcccggc cccggctggg
     1141 ccgcgccgag cgaccccggc tccaccgtgc cgctggaccc cgcgcgcacc gcgccgcccg
     1201 accccgcgcg caccgcgccg cccgatcggg gcgggagcgg ccggcccggc ccggacgagc
     1261 ctccagggcc gtccgcggat cagcccgatg aggaggccgg cccggcggcg ggcgggttcg
     1321 tgcctccagg ctgagcgccg cgcaccagcc cgcgtcgagc gcggggcagc agcccgcacc
     1381 ccgggcctgt ggacgtcctg tggacggcgt gtggacgacc ggatggccag gcgcgaagat
     1441 cggagcatgg gctacgacct caccgcgctg atcggggcgc cagacacgct gcggcccgcg
     1501 gtcgcggccg tgcccggcgc ccgggtcgtc gacatcgccg ccgggctcgc gctcgtcccg
     1561 ctcaccgggc cggtgctggg cgccatccgg gccggtggcg gcgagcgggc cgtcgcgccg
     1621 gcgcgcgggt tccaggcgct cacccccgcg gtggcgcgct tcgcggccgg cgcctcggcc
     1681 gccggcccgg tgctctacgc cgaggccgag ttcttcgggg ggtcgggctc ccaggccgtg
     1741 atcggctggc gcggcgggcg ggtctccatc ggcccgctgc tcacccagac catgggcgag
     1801 ggtcgcgagg ggttcgccgt ggcggccggg cccgggctgc gggggatggc gctcaacgca
     1861 gggctgcggg accttggcgt ccgcacgccg gccgacggcg agccgtggca ggacgagttc
     1921 caggcagccg gcctcggccg gtgccgcgcc accgaggact ggtcgtgacc gctcccgggt
     1981 ccgcccgtcg gtcaggtgcc ctcggtccag gagtggaggt actcgacctg ggtgtcggtg
     2041 agggtgtcga tctcgatgcc catggtcgcg agcttcaccc gcgcgacctc ggcgtcgatc
     2101 tcggccggga cgtcgtggac gcggacctcg agggtgtcct ggcgggcgag ccactcgagc
     2161 gacagcgcct ggtcggcgaa cgacatgtcc atgaccgcgg ccgggtgccc ctcggcggcc
     2221 ccgaggttca ccagccgccc ctcggcgacc aggttgatcc ggcggccgtc ggcagcctgg
     2281 aactcctcga cgttctcgcg cacccgggtc accgaggtgg ccagctcgcg gagggcgacc
     2341 ttgtcgatct cgacgtcgaa gtggccggag ttggccagga cgacgccgtc ccgcatcacc
     2401 tggaagtgct cgcggcggag gatgtcccgg tcgccggtga ccgtgacgat cacgtcggcc
     2461 tcgcgggcgg cctcggccat cggggcgacg cggtagccct ccatggccgc ctcgagcgcc
     2521 cgcacggggt cgacctcggt cacgaccacc ttggccccga ggccgcgggc gcgcatggcg
     2581 aggcccttgc cgcagtagcc gtacccggcg acgacgaacg tcgtgcccgc gagcaggagg
     2641 ttggtcgccc gcaggatgcc gtcgatggtc gactggccgg tgccg
//
LOCUS       DASPYY010000242         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2432197, whole genome shotgun sequence.
ACCESSION   DASPYY010000242 DASPYY010000000
VERSION     DASPYY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432197"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VEP73_03665"
     CDS             complement(<1..255)
                     /locus_tag="VEP73_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYT25566.1"
                     /translation="MTPYFRQHRPPALVATLPPIVDHGALAGTPRTVVGAFPDLVSSG
                     QAVAQITRRGLTPAVLELLDRACLEAVEEWKHLGIDADAAA"
     gene            173..1162
                     /locus_tag="VEP73_03670"
                     /pseudo
     CDS             173..1162
                     /locus_tag="VEP73_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
     gene            1322..>1765
                     /locus_tag="VEP73_03675"
     CDS             1322..>1765
                     /locus_tag="VEP73_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HYT25567.1"
                     /translation="MAQPARAHIRLAELLGVLSFGADLGMGQPMEHVLRQCLISLRLA
                     ERMGLDKADREVVYYTSLLAWVGCHVDAYEQAKWFGDEMALKTDFRHTDFGAVAAQPL
                     FILRHLGAGRPMVERARLGATFAGDGRRVAEAMIENHWLAADGLGE"
BASE COUNT          228 a          642 c          649 g          246 t
ORIGIN      
        1 cgccgccgcg tccgcgtcta tgcccaggtg cttccactcc tccaccgcct ccaggcaggc
       61 ccggtccagc agctcgagga ccgcgggggt cagcccgcgc cgagtgatct gggccacggc
      121 ctgcccggag gagaccaggt cggggaaggc cccgacgacg gtccgcggcg tgccggccag
      181 cgcgccgtgg tccactatcg gcggcaacgt ggccaccaat gcgggcggcc tgtgctgcct
      241 gaagtacggc gtcacccgcg actacgtgct cggtctgcgc gcggtcgtcg gcggaccggt
      301 cgcctacggc gacgcggtcc ggctggggcg gcgcactacc aagggcgtcg ccgggctcga
      361 cctagtgggc ctgttcgtcg ggtccgaggg cacgctcggc gtggtgaccg aggtgaccct
      421 gcggctgcgg cccgcgccgg cgggcacgcc gcggaccgtc gtcggggcct ttcccgatct
      481 ggtctcctcc gggcaggccg tggcccagat aacccgccgc gggctgaccc ccgcggtgct
      541 cgagctgctg gaccgcgcct gcctggaggc ggtggaggag tggaagcacc tcggcatcga
      601 cgcggacgcg gcggcgctgc tgctggcccg ggcggacacc ccaggagact cgggcgcggc
      661 cgaagcgtcc gcgatcgccg ccaccatgac cgcggcgggc gcgctgtggg cagagcagtc
      721 caccgacgac gccgaggccg aggcgctgtt cgacgccagg cggctggctt accccgcgct
      781 cgagcggctc ggcccggttc ttaccgaaga cgtttgcgtg ccccggtccc aagtgcccgc
      841 gatgctggag cagatcacta cgatcgcggc ccggcacggc gtgcggaccg ccaccgtcgc
      901 gcacgccggc gacgggaacc tgcacccgtg cctgatcacg ccgccgggcg attcggccgc
      961 ccgggaagcc gcccaggcgg cgttcgagga attcctcgac gcggccatcg cgctgggcgg
     1021 cacggtgacc ggggagcacg gcgtcggcat cctgaaacgg gacggcatgc gccgtgagct
     1081 cgacccgggc tcgctcgcgc tgcaggccgc agtgcgccgc gcactcgacc cgcgcgagat
     1141 tttcaacccc ggcaagggct agtagtcctc ggtgacggcg ggctcgcagg gcaacggctg
     1201 cgcgaggccc tcgccctggc cgccgcccca tcgctggggc cagggccgac gaaatgacga
     1261 tttgtcgcta aatcgccctg acctgctgcc tcgcgcatgg ctccggctta tattcttcgc
     1321 tatggcccag cccgcccgcg cgcacatccg cctggccgaa ctgctcggcg tgctgtcgtt
     1381 cggcgccgac ctgggcatgg gccagccgat ggaacacgtc ctgcgccagt gcctgatctc
     1441 gctgcggctg gccgagcgga tgggcctgga caaagccgac cgggaggtcg tgtactacac
     1501 gtccctgctg gcctgggtgg gctgccacgt cgacgcctac gagcaggcga agtggttcgg
     1561 cgacgagatg gccttgaaga ccgacttccg gcacaccgac ttcggcgcgg tcgccgctca
     1621 gccgctgttc atccttcggc atctcggcgc gggccggccc atggtggagc gcgcccggct
     1681 cggggcgacg ttcgcgggtg acggccgccg ggtggccgag gccatgatcg agaaccactg
     1741 gctggcggcc gatggcctcg gcgag
//
LOCUS       DASPYY010000243         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_886333, whole genome shotgun sequence.
ACCESSION   DASPYY010000243 DASPYY010000000
VERSION     DASPYY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886333"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1900
                     /locus_tag="VEP73_03680"
     CDS             224..1900
                     /locus_tag="VEP73_03680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HYT25568.1"
                     /translation="MSGTAGARAAEAVEGHGGTLAVAVARRHGIDTMFTLSGAHVFPL
                     YDGAVKADPPMRLVDVRHEQTAVFAAEATAKLTRVPGLAVLTAGPGVTNGVSAVTTAH
                     FNGSPLLVLGGRAPAGRWGAGSLQELDHPPLLAPVTKHAATVGEVSGLPAAVDRALAL
                     AAAPHRGPVFLDVPMDQLFSFATVPIPVPAGPQPLVPDPDALAGVAALLAESERPVLV
                     LGSDVWADRAEGAARRAAETLRLPVVANGMGRGIVPPGAGLAVTRARSLAFRAADLVL
                     VAGAPLDFRLGFGSFGGEGGVPARVVHLADAPSQVARHVDLAGTVAGDLSLVLDGLVA
                     AAERLGRRTRREEWLDRLRGTARKAAEDDRELLESGADPIHPARVYGELRRVLADDAV
                     VAGDGGDFVSFAGRYLEPARPGGWLDPGPYGCLGTGLGYAVAARLARPAAQVVLMLGD
                     GAAGFSLMDADTLVRHGLPVVMVCGNNGIWGLEKHPMQALYGYDVAADLQPGCRYDQV
                     VAALGGGGELVASPDGIGPALHRAFDAGVPYLVNVVTDPRAAYPRATSGI"
     gene            2041..2868
                     /locus_tag="VEP73_03685"
     CDS             2041..2868
                     /locus_tag="VEP73_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HYT25569.1"
                     /translation="MERDEADRLLRSPSLADGATLTGEPGSGEEGARPGSLLQPLRLR
                     SAGEQIAERLLTAIALGEFPPGQRLPTERDLADVLGVSRTSVREALHRLAAGGYVEVQ
                     RGRYGGAVVQAGWGPATDGLTWRTVRQDRERLEWLFDFRSLVEPLVARVAAERRGPGD
                     AAAVEGALAGYRRAGGPEGHRAADQALHDAVARATHNPYLVGICRQVRVQLSLAFGAM
                     PHDRSLRDRALAEHTALARAVVAGDPDGAARAAGEHATSTEPSVRELRRRMEEEGRG"
     gene            2961..>3243
                     /locus_tag="VEP73_03690"
     CDS             2961..>3243
                     /locus_tag="VEP73_03690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HYT25570.1"
                     /translation="MRDGEPEAGLFGPGSVSWQVLREASVMIGGVRALLMHAAHPLVV
                     AGARQTGMYERDPWRRLARTLRQTFTVVFGTSEEAAAASRRIDDVHRAIR"
BASE COUNT          361 a         1241 c         1242 g          399 t
ORIGIN      
        1 ctgggcgtgt tcgcctacaa cctgcagcgc atgaccgtgg tcacctattg agccgacggc
       61 aggcagccac gccagcgtcg ctgccacacc ccgcccacct caacccctga gcaggacttt
      121 ttcatgggga agtagctagc cgcggcgccg ccgctggccg ggcgcttccg cgctcggcac
      181 ccgcccgcta gcatcgcttc gaccggtggg catggaggtg ggcgtgagcg ggacggcagg
      241 ggcgcgggcc gcggaggccg tcgaggggca cggcggcacg ctggccgtcg ccgtcgcgag
      301 gcgccacggc atcgacacga tgttcacgct ctcgggcgcc cacgtgttcc ccctctacga
      361 cggcgcggtg aaggccgacc cgccgatgcg gctcgtcgac gtgcgccacg agcagaccgc
      421 ggtgttcgcc gccgaggcga ccgccaagct gacccgcgtg cccgggctcg ccgtgctgac
      481 cgcggggccg ggggtcacca acggcgtcag cgccgtgacg acggcgcatt tcaacggctc
      541 gccgctgctc gtcctcggcg ggcgggcgcc ggccggccgc tggggcgccg gcagcctgca
      601 ggagctcgac cacccgcccc tgctcgcccc ggtgaccaag cacgccgcga ccgtcggcga
      661 ggtctcggga cttccggccg cggtcgaccg ggcgctcgcg ctcgcggccg cgccgcaccg
      721 tgggccggtg ttcctcgacg tgccgatgga ccagctgttc tccttcgcca ccgtgcccat
      781 cccggtgcct gccgggccgc agcccctggt gcccgacccg gacgcgctcg ccggcgtggc
      841 cgcgctgctc gcggagtccg agcggccggt gctggtgctc ggctcggacg tgtgggccga
      901 ccgggccgag ggcgccgcca ggcgcgcggc cgagacgctc cggctcccgg tggtcgccaa
      961 cgggatgggc cgcgggatcg tcccccccgg cgccggcctg gcggtgacgc gggcgcgctc
     1021 gctcgccttc cgggcggccg acctcgtgct ggtcgccggc gccccgctcg acttccggct
     1081 cggtttcggg tcgttcggcg gcgagggcgg cgtcccggcc agggtcgtgc acctcgccga
     1141 cgcgccgagc caggtcgcgc ggcacgtcga cctcgcgggc acggtcgccg gcgacctctc
     1201 gctggtgctc gacgggctcg tggccgcggc cgagcggctc gggcgccgga cgcgccggga
     1261 ggagtggctg gaccggctgc gcggcacggc ccgcaaggcc gccgaggacg accgggagct
     1321 gctcgagagc ggcgccgatc ccatccaccc ggcccgcgtc tacggggagc tgcgccgcgt
     1381 gctggccgac gacgccgtcg tcgcgggcga cgggggcgac ttcgtgtcct tcgccggcag
     1441 gtacctcgag ccggcgcggc caggcggctg gctcgaccca ggaccgtacg gctgcctcgg
     1501 caccggcctc ggctacgcgg tggccgcgcg gctggcccgg cccgccgccc aggtcgtgct
     1561 catgctcggc gacggggcgg ccggcttctc gctgatggac gccgacacgc tggtgcgcca
     1621 cgggctgccg gtcgtcatgg tgtgcggcaa caacgggatc tgggggctcg agaagcaccc
     1681 gatgcaggcg ctctacggct acgacgtcgc ggccgacctg cagcctggct gccggtacga
     1741 ccaggtcgtc gcggcgctcg gcggcggcgg ggagctggtc gcgtccccgg acgggatcgg
     1801 gccggcgctg cacagggcgt tcgacgccgg cgtgccctac ctcgtgaacg tcgtcaccga
     1861 ccccagggcc gcctacccga gggcgaccag cgggatctga atcgtaccgg gtgccgccag
     1921 atccgccgcc ccatcccaag ttgcccggac tctgttagct tcccgaggcg cgcaacatcc
     1981 acggcactga cgaggcggtc gagctcgtcc cctccgtgaa gggagcgtcg accagccgac
     2041 atggaacgcg acgaggccga caggctactg cggtcgccgt ccctggcgga cggcgccacg
     2101 ctgaccgggg agcccggctc gggcgaggag ggtgcccgcc ccggctccct cctgcagccg
     2161 ctccggctcc ggagcgccgg cgagcagatc gccgagcgcc tgctgaccgc gatcgccctc
     2221 ggcgagttcc cgccgggcca gcggctcccc accgagcgcg acctcgccga cgtcctcggg
     2281 gtgagccgga cctcggtccg cgaggcgctc caccgcctcg ccgcgggcgg ctacgtcgag
     2341 gtgcagcgcg gccggtacgg cggcgcggtc gtacaggccg gctgggggcc ggccacggac
     2401 gggctgacct ggcgcacggt ccggcaggac cgggagcgcc tcgagtggct gttcgacttc
     2461 cggagcctcg tcgagccgct ggtcgccagg gtcgcggccg agcgccgtgg ccctggcgac
     2521 gccgccgccg tcgagggggc gctcgccggc taccggcggg ccgggggccc cgagggccac
     2581 cgggccgccg accaggctct ccacgacgcc gtcgccaggg ccacgcacaa cccctacctc
     2641 gtcggcatct gccggcaggt ccgcgtccag ctcagcctcg cgttcggcgc catgccgcac
     2701 gaccgctcgc tccgcgaccg ggcgctcgcc gagcacaccg cgctcgcccg tgccgtcgtg
     2761 gccggcgacc cggacggggc ggcccgcgcc gccggggagc acgccacctc gaccgagccg
     2821 tcagtccgcg agctgcgccg gcggatggag gaggaagggc gcgggtgacc acgcaccggg
     2881 acgcgcgctg ccgcgcggcg gggcccactt gcctggagcg cgcggggccg gtcgccctat
     2941 cctggtcgta tcctggtcgc atgcgcgacg gtgagccgga agccgggctg ttcgggcccg
     3001 gcagcgtctc ctggcaggtc ctgcgggagg cctcggtcat gatcggcggc gtgcgggcac
     3061 tgctcatgca cgccgcccac ccgctcgtgg tcgccggagc caggcagacc ggcatgtacg
     3121 agcgggatcc gtggcggcgg ctcgcgcgga ccctgcgcca gacgttcacg gtggtgttcg
     3181 ggaccagcga ggaggcggcc gcggcgtcgc ggcggatcga cgacgtgcac cgggcgatca
     3241 ggg
//
LOCUS       DASPYY010000244         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1871267, whole genome shotgun sequence.
ACCESSION   DASPYY010000244 DASPYY010000000
VERSION     DASPYY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871267"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VEP73_03695"
     CDS             <1..403
                     /locus_tag="VEP73_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25571.1"
                     /translation="VVVLIIAILIAIAIPTFLGARQRAQDRAAQSNLRNALTAEKTYY
                     TDHQAYTETASDLTAIEPSLTYDAVKDTSPTQGSIGYVLVSGAIVLASKSASGTCFYI
                     KDDPSNAGTGTKYGKGACAQPSTVAIALTNW"
     gene            516..992
                     /locus_tag="VEP73_03700"
     CDS             516..992
                     /locus_tag="VEP73_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYT25572.1"
                     /translation="MARGRRPRGRDEGFTLIELMVVVLIIAILIAIAIPTFLGARQRA
                     QDRAAQSSLRNALTAAKAIYADQQDYTKADVATLTAEEKNLTFQAAASTGANEISLGA
                     GVSTTQVFYAAAQSASGTCFYVKDDAGAGGGTGYGSDTPASCAATATPTGGYVSKW"
     gene            1097..>1775
                     /locus_tag="VEP73_03705"
     CDS             1097..>1775
                     /locus_tag="VEP73_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HYT25573.1"
                     /translation="MSPLGAAVLAGLLGLAFGSFANVVIHRVPRRQSVVRPASRCPAC
                     GTPIAARDNVPVLGWLLLRGRCRRCLAPISPGYPAVEATMAALWALVAYRLWSDGLAW
                     AVPAYLAVAFVCVVLTVIDARSRLLPNRITYPAFPAVGTLLLVASLALHDLGRMQRGL
                     FAALAVTVLFLLLFLVYPGGMGLGDVKFVPTLGLALGWLSWPVLAVGIFAAFLLGGVI
                     GVAAIVVL"
BASE COUNT          219 a          707 c          599 g          250 t
ORIGIN      
        1 ggtcgtggtg ctcatcatcg ccatcctgat cgcgatcgcc atcccgacct tcctcggcgc
       61 ccgccagcgc gcccaggacc gcgccgccca gtccaacctc cgcaacgcgc tcacggcgga
      121 gaagacctac tacaccgacc accaggccta caccgagacc gcctcggacc tgaccgcgat
      181 cgagccgtcc ctgacctacg acgcggtgaa ggacacctcg ccgacccagg gctcgatcgg
      241 ctacgtgctg gtctcgggcg cgatcgtcct ggccagcaag agcgcctcgg gcacctgctt
      301 ctacatcaag gacgacccga gcaacgccgg cacggggacc aagtacggca agggcgcgtg
      361 cgcccagccg agcacggtcg ccatcgcgct gaccaactgg taggcggccc gagccgaggc
      421 accgaccgcc ggggcggccg ctcgtcggcc gccccggcgg ttctcccggg cgaagcgaaa
      481 ggggcatggt gaacatggcg caatggaggt cccgcgtggc gcggggacgg cgcccgcgag
      541 ggcgcgacga gggcttcacc ctcatcgagc tgatggtcgt ggtgctcatc atcgccatcc
      601 tgatcgcgat cgccatcccg accttcctcg gcgcccgcca gcgcgcccag gaccgcgccg
      661 cccagtcgag cctccgcaac gccctcaccg cggccaaggc gatctacgcc gaccagcagg
      721 actacaccaa ggccgacgtc gccaccctca ccgccgagga gaagaacctg accttccagg
      781 cggcagcctc gaccggcgcg aacgagatct cgctcggcgc cggcgtcagc accacgcagg
      841 tcttctacgc cgcggcgcag tccgcctccg gcacctgctt ctacgtcaag gacgacgccg
      901 gcgcgggcgg cggcaccggc tacggcagcg acacccccgc gagctgcgcg gcgaccgcga
      961 ccccgaccgg cgggtacgtc tccaagtggt gaccggccgg tgcggctgac cgggccggcc
     1021 gccccccgcg gggcggccgg cccgccggct gtggacggcg gcgccgcggg cgctccggag
     1081 cggtaggctc ccgcccatga gccccctcgg tgccgccgtc ctggccgggc tgctcggcct
     1141 tgcgttcggg tcgttcgcga acgtggtgat ccaccgcgtc ccccggaggc agtcggtcgt
     1201 ccggcccgcc tcgcgctgcc cggcgtgcgg gaccccgatc gcggcccgcg acaacgtccc
     1261 ggtgctcggc tggctgctcc tgcgcggacg ctgccgccgg tgcctggcgc ccatctcgcc
     1321 cggctacccg gccgtggagg cgacgatggc cgcgctctgg gcgctcgtcg cctaccggct
     1381 gtggtccgac gggctggcgt gggcggtccc cgcctacctg gccgtcgcct tcgtctgcgt
     1441 cgtgctcacc gtgatcgacg cccggtccag gctcctgccg aaccggatca cctacccggc
     1501 cttcccggcg gtcggcacgc tgctgctcgt ggccagcctc gcgctccacg acctcggccg
     1561 gatgcagcgc ggcctgttcg ccgccctggc cgtcaccgtc ctgttcctgc tgctcttcct
     1621 cgtctacccg gggggcatgg gcctcggtga cgtcaagttc gtccccaccc tcgggctcgc
     1681 gctcggctgg ctgtcgtggc cggtgctcgc ggtcgggatc ttcgccgcgt tcctgctcgg
     1741 cggggtgatc ggggtcgcgg ccatcgtcgt gctgc
//
LOCUS       DASPYY010000245         5351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2712998, whole genome shotgun sequence.
ACCESSION   DASPYY010000245 DASPYY010000000
VERSION     DASPYY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712998"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VEP73_03710"
     CDS             <1..568
                     /locus_tag="VEP73_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYT25574.1"
                     /translation="SPAVLEAVTGFHVHRGALASVARPPLPDAPTLLAGACRLAVLED
                     VNNPTNLGALFRSAAALGMGGVLLSPGCADPLYRRAVRVSMGEVLAVPYARLAPWPAA
                     LAEVRAAGFELLALTPAAGAEPLDRVEVGPRDRVAVLLGAEGPGLTPAALAAAGRAVR
                     IPMAAGVDSLNVAAAAAVAFWVLGRRRA"
     gene            complement(611..1636)
                     /locus_tag="VEP73_03715"
     CDS             complement(611..1636)
                     /locus_tag="VEP73_03715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYT25575.1"
                     /translation="MTDRVPSDVADRPREPVTRFGLQIPTFTYPGVPDAELFERVAAI
                     ATTAEQAGFDSLWVMDHLYQIRTVGPPTDPMLESYTLLGALAARTERARLGALVTGVT
                     YRNPALLAKIVTTLDVISSGRAILGIGAAWNEDEHAGYGFDFPPPGERLDRLEEALRI
                     CRAMFTEEAPTVAGRHARIQGALNRPRPVTPGGPPILVGGGGERRTLRLVARYADACN
                     LFGDVATIRRKLEVLDRHCQEVGRDPAEISRTRLGTLVISPTAGEAARRAEQLRVAMG
                     VDEATFGAMVVAGDPDAVAEQVAAYLDAGLDGLMFNMPGAETLEPVALAGETLVKHFG
                     PGGTARR"
     gene            complement(1656..3035)
                     /gene="hydA"
                     /locus_tag="VEP73_03720"
     CDS             complement(1656..3035)
                     /gene="hydA"
                     /locus_tag="VEP73_03720"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HYT25576.1"
                     /translation="MRTLIQGGTVVTAADTMRADVLVDGEKVAAVGTGLAASADRVVD
                     ATGRLVMPGAIDVHTHMEMPFGGTFSSDDFASGTAAAAWGGTTTIIDFAMHDYGETLQ
                     AGLDRWHEKAGPKAHVDYGFHMIVREVNDQTLADMDALVGRGVPSFKLFMAYPGVFMV
                     DDAALFRAMSRTSENGGLIMLHAENGGPIDVLIERYLAEGRTDPVNHGLTRPASMEGE
                     AVHRAVALADLAGVAVYIVHLSSADALDEVREARDRGLTTLAETCPQYLYLSLDDLGR
                     EGFEGAKYVCSPPLRPLPHQAELWKGLRQDDLQVVSTDHCPFHFKDQKELGRGNFAKI
                     PNGLPGVEDRFTLLYDGGVRAGHISANRFVELVATAPAKLFGLWPRKGTVAPGSDADV
                     VVFDPDAERVLSAATHHMNVDYSCYEGRRVHGVPEVVMQRGEVLVDHGEFLARPGQGR
                     FLERSRSGL"
     gene            complement(3070..4392)
                     /locus_tag="VEP73_03725"
     CDS             complement(3070..4392)
                     /locus_tag="VEP73_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYT25577.1"
                     /translation="MTADANGSGNDPATLRARHARVLPAWLTLYYDEPIELVRGEGVH
                     VWDSAGNRYLDFFGGILTTISGHGVPELTEVLVDQAGKINHTSTLYLIRSMVELAERL
                     TELAPVDPPTKAFFTTSGSEANEAALLFATGYRRSNEVIALRNSYHGRSFAAIGITGQ
                     KSWSASSYSPFQVSYALTPYCYRCPFGLTYPSCGVACAEDLRNVIETTTTGAPAAMIA
                     EPIQGVGGFVTPPREYFGIVKSILDDFGIPLIADEVQTGFGRTGEAFWGIESYGVRPD
                     AIVMAKGLGNGLAIGAVVAKAEWVDSLRANHISTFGGGPITSRYAVANLDYIQRQDLQ
                     ANAARMGRILMDGLKPLETESPIVGEVRGKGLMVGVELVTDHDSKEPNVKAAAEVMER
                     CRERGLLVGKGGLYANTLRIAPPLTVTEQDVGQAVETIRAAVGQAQGA"
     gene            complement(4389..5234)
                     /locus_tag="VEP73_03730"
     CDS             complement(4389..5234)
                     /locus_tag="VEP73_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HYT25578.1"
                     /translation="MTTVRAAIIQAHANMPKDEAVEKHVGLIAKAAAEGAQITCLQEI
                     FHGPYFCAEQDPRWYATAEPEDGPTVTRMRELARQHRMALVVPWFEEAQTGVYYNSAA
                     VIERDGTLLGKYRKTHIPQVGPCFFEKYYFKPGNLGYPVFDTSVGRVGLIICYDRHFP
                     EVARQVGLKGAELVFNPSATVESLSRYLWELEQPAHAVANGYWVAAINRVGVEAPLNP
                     NKFYGSSYFCDPRGQIVAQASDSEDEVLVADLDLDRIREVRNTWQFMRDRRPETYREL
                     TELLP"
BASE COUNT          663 a         1907 c         1990 g          791 t
ORIGIN      
        1 gagcccggcc gtgctcgagg cggtgaccgg gttccacgtc caccgcggcg cgcttgcgtc
       61 ggtcgcgcgg ccgcccctgc ctgacgcgcc gacgctgctg gccggtgcgt gccggctggc
      121 cgtgctggag gacgtcaaca acccgaccaa cctgggcgcg ctgttccggt ccgcggccgc
      181 cctcggcatg ggcggcgtgc tgctcagccc gggctgcgcc gacccgctct accgccgggc
      241 ggtgcgcgtg tcgatggggg aggtcctcgc cgtgccgtac gcccggctcg cgccgtggcc
      301 ggccgccctg gccgaggtgc gcgcggccgg cttcgagctg ctggcgctga ccccggccgc
      361 cggcgccgag ccgctcgacc gggtcgaggt cggcccgcgg gaccgcgtcg cggtgctgct
      421 cggcgccgag gggccggggc tgaccccggc cgcgctggcc gcggccggcc gcgcggtccg
      481 catcccgatg gccgccggcg tcgactccct caacgtggcc gccgccgcgg ccgtggcctt
      541 ctgggtcctg ggtcgccgcc gggcctgacc gccgggcctg accgcctccc gccgccgccg
      601 gcctgaccgt ctaccggcgt gccgtcccgc ccggcccgaa gtgcttcacc agggtctcgc
      661 cggccagcgc gactggttcg agggtctccg cgcccggcat gttgaacatg agcccgtcca
      721 ggccggcgtc gaggtaggcg gcgacctgct cggccaccgc gtccgggtcg cccgcgacca
      781 ccatggcgcc gaacgtggcc tcgtcgacgc ccatggccac ccggagctgc tccgccctgc
      841 gcgccgcctc gccggcggtc ggggagatga cgagcgtccc gaggcgggtc ctcgagatct
      901 cggccgggtc gcgcccgacc tcctggcagt ggcggtcgag cacctcgagc ttgcgcctga
      961 tggtcgcgac gtcgccgaag aggttgcagg catcggcgta ccgcgcgacg agccggagcg
     1021 tgcggcgctc gccgccgccg ccgacgagga tcggggggcc gcccggggtc accgggcggg
     1081 gccggttgag ggcgccctgg atgcgcgcgt ggcgtccggc caccgtcggc gcctcctcgg
     1141 tgaacatcgc tcggcagatc cgcagggcct cctccaggcg gtcgaggcgc tcgccgggcg
     1201 gggggaagtc gaagccgtag ccggcgtgct cgtcctcgtt ccaggccgcc ccgatgccga
     1261 ggatggcccg gccggacgag atcacgtcga gggtggtgac gatcttggcc agcagcgccg
     1321 ggttgcggta ggtcacgccg gtgacgagcg cgcccagccg ggcacgctcg gtccgtgccg
     1381 ccagcgcgcc gagcagcgtg taggactcga gcatcgggtc ggtgggcggc ccgacggtgc
     1441 ggatctggta gagatggtcc atcacccaga gcgagtcgaa gccagcctgc tcggcggtcg
     1501 tggcgatcgc cgcgacgcgc tcgaagagct cggcgtccgg gacgcccggg taggtgaagg
     1561 tcgggatctg caggccgaac cgggtgaccg gctcgcgggg gcggtcggcg acgtcgctcg
     1621 gtacccggtc ggtcatcggt cgtctccctt gtcgtctaca ggccggaccg gctgcgctcg
     1681 aggaaccggc cctgcccggg ccgggcgagg aactcgccgt ggtcgaccag cacctcgccg
     1741 cgctgcatga ccacttctgg caccccgtgg acgcgcctgc cctcgtagca ggagtagtcg
     1801 acgttcatgt ggtgcgtggc ggccgagagc acccgctcgg cgtcggggtc gaacacgacg
     1861 acgtcggcgt cggacccggg cgcgaccgtg cccttccgcg gccacaggcc gaacagcttg
     1921 gcgggggcgg tcgccaccag ctcgacgaac cggttggcgc tgatgtgacc ggcccggacg
     1981 ccgccgtcgt acaggagcgt gaagcggtcc tcgaccccgg gcagcccgtt cgggatcttg
     2041 gcgaagttgc cgcgtcccag ctccttctgg tccttgaagt ggaacgggca gtggtcggtc
     2101 gagacgacct gcaggtcgtc ctggcggagc cccttccaca gctccgcctg gtgcggcagg
     2161 ggccgcagcg gcggcgagca gacgtacttc gcgccctcga acccctcgcg gccgaggtcg
     2221 tcgaggctca ggtagaggta ctgcgggcag gtctcggcga gcgtcgtgag gccccggtcg
     2281 cgcgcctcgc ggacctcgtc cagcgcgtcg gccgacgaca ggtggacgat gtagacggcg
     2341 acgccggcga ggtcggccag ggcgaccgcc cggtgcaccg cctcgccctc catcgaggcg
     2401 gggcgggtca gcccgtggtt gaccgggtcg gtgcggccct cggccaggta gcgctcgatg
     2461 agcacgtcga tcgggccgcc gttctcggcg tggagcatga tgagcccgcc gttctccgag
     2521 gtccggctca tcgcccggaa cagcgcggcg tcgtcgacca tgaagacgcc cgggtaggcc
     2581 atgaacagct tgaacgacgg caccccgcgg ccgaccaggg cgtccatgtc ggccagggtc
     2641 tggtcgttca cctcccggac gatcatgtgg aagccgtagt cgacgtgcgc cttcggcccg
     2701 gccttctcgt gccaccggtc gagcccggcc tgcagggtct cgccgtagtc gtgcatcgcg
     2761 aagtcgatga tcgtggtggt gccgccccag gcggccgcgg ccgtgccgct cgcgaagtcg
     2821 tcggacgaga aggtcccgcc gaacggcatc tccatgtgcg tgtggacgtc gatcgcgccg
     2881 ggcatcacga gccggccggt cgcgtcgacg acccggtccg cgctcgcggc gaggccggtg
     2941 ccgaccgcgg ccaccttctc gccgtcgacc agcacgtcgg cccgcatcgt gtccgccgcg
     3001 gtcaccaccg tgccgccctg gatgagggtt cgcactccgt gcacctcctg tcgagtggtc
     3061 ggcgcggcct caggcgccct gcgcctgccc gaccgcggcg cggatggtct cgaccgcctg
     3121 gccgacgtcc tgctcggtca cggtcagcgg gggcgcgatc cgcagggtgt tggcgtagag
     3181 gccgcccttg ccgacgagca ggccacgctc ccggcagcgc tccatcacct cggccgccgc
     3241 cttcacgttg ggctccttcg agtcgtggtc ggtcaccagc tcgacgccga ccatgaggcc
     3301 cttgccgcgc acctccccga cgatcggcga ctcggtctcc agcggcttca gcccgtccat
     3361 caggatccgg cccatccggg ccgcgttcgc ctgcaggtcc tggcgctgga tgtagtccag
     3421 gttggcgacc gcgtagcggg aggtgatcgg cccgccgccg aaggtcgaga tgtggttggc
     3481 gcgcagcgag tcgacccact ccgccttggc caccaccgcg ccgatcgcga ggccgttgcc
     3541 gagccccttg gccatgacga tggcgtccgg ccgcaccccg tagctctcga tgccccagaa
     3601 cgcctcgccg gtgcgcccga acccggtctg cacctcgtcg gcgatcagcg ggatgccgaa
     3661 gtcgtcgagg atcgacttca cgatcccgaa gtactcccgc ggcggggtga cgaagccgcc
     3721 gacgccctgg atcggctcgg cgatcatcgc cgccggcgcg ccggtggtgg tggtctcgat
     3781 cacgttgcgc aggtcctcgg cacacgcgac cccgcagctc gggtacgtga ggccgaacgg
     3841 gcagcggtag cagtacgggg tgagcgcgta ggagacctgg aaggggctgt aggacgaggc
     3901 cgaccaggac ttctgcccgg tgatcccgat cgccgcgaac gagcggccgt ggtagctgtt
     3961 gcgcagggcg atgacctcgt tggaccggcg gtagccggtc gcgaacagca gggccgcctc
     4021 gttggcctcc gaccccgacg tcgtgaagaa cgccttggtg ggcgggtcga ccggggcgag
     4081 ctcggtgagc cgctcggcca gctccaccat ggagcggatg aggtagaggg tcgacgtgtg
     4141 gttgatcttg ccggcctggt cgacgaggac ctcggtgagc tcgggcacgc cgtggccgga
     4201 gatggtggtc aggatgccgc cgaagaagtc gaggtagcgg ttgccggccg agtcccagac
     4261 gtggacgccc tcgccccgca ccagctcgat cggctcgtcg tagtagaggg tgagccacgc
     4321 cggcagcacg cgcgcgtgcc tggcccgcag cgtggccggg tcgttcccgc tcccgttcgc
     4381 gtcggccgtc acggcaggag ctccgtcagc tcgcggtagg tctccgggcg ccggtcgcgc
     4441 atgaactgcc aggtgttgcg gacctcgcgg atccggtcga ggtcgaggtc ggcgaccagc
     4501 acctcgtcct cggagtcgga cgcctgcgcg acgatctggc cgcgcgggtc gcagaagtac
     4561 gacgagccgt agaacttgtt cgggttcagc ggcgcctcga ccccgacccg gttgatggcg
     4621 gcgacccagt agccgttggc gaccgcgtgg gccggctgct cgagctccca caggtaccgt
     4681 gacagcgact cgaccgtcgc cgacgggttg aagaccagct cggcgccctt gaggccgacc
     4741 tgccgggcga cctcggggaa gtggcggtcg tagcagatga tcagccccac ccggccgacc
     4801 gaggtgtcga acaccgggta gccgaggttg cccggcttga agtagtactt ctcgaagaag
     4861 caggggccga cctgcggaat gtgggtcttg cggtacttgc cgagcagggt gccgtcccgc
     4921 tcgatcaccg cggccgagtt gtagtagacg ccggtctggg cctcctcgaa ccaggggacg
     4981 accagcgcca tccggtgctg gcgggcgagc tcgcgcatgc gggtgacggt cggcccgtcc
     5041 tccggctcgg cggtcgcgta ccacctggga tcctgctcgg cgcagaagta ggggccgtgg
     5101 aagatctcct gcaggcaggt gatctgggca ccttcggcgg ccgccttggc gatcagcccg
     5161 acgtgcttct cgaccgcctc gtccttgggc atgttggcat gggcctggat gatggccgcc
     5221 cggaccgtgg tcatggggtc gctccttgct cgctgctcgc ggtccgccct tgcgcgccgc
     5281 cggccgcccg ggcggcgggg ccgagcacgg ggatcagctc cttgccgaac gcgcggatca
     5341 cgccctccgg g
//
LOCUS       DASPYY010000246         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_43446, whole genome shotgun sequence.
ACCESSION   DASPYY010000246 DASPYY010000000
VERSION     DASPYY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43446"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VEP73_03735"
     CDS             <1..202
                     /locus_tag="VEP73_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25579.1"
                     /translation="AVDDRIDLAVKAEGEVAEALDAHRDHVLGETLATSLHRAPQGDG
                     YDARVELDGQTVRLWLRPRPRA"
     gene            211..681
                     /locus_tag="VEP73_03740"
     CDS             211..681
                     /locus_tag="VEP73_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25580.1"
                     /translation="MRARDDGIGGLLPAARPWWGYLWAAPNSLVGLVGAASSGGRPVR
                     WRGVLLCEGGRAGLARFLAWRGFCAITLGHVIVASEPLDDHLLAHELGHVRQHERWGP
                     LFYPAYLLASLAGYRRNPFERAAERRAVHALAHQAGPAAHSPGEPGDRPGVGRA"
     gene            674..1144
                     /locus_tag="VEP73_03745"
     CDS             674..1144
                     /locus_tag="VEP73_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HYT25581.1"
                     /translation="MPDRRGARPGHADYSGTPLPRKLGIRPGATVALLDAPEGAGDLL
                     GPLPDGVVVRRRAQGGLDVVVLFVTSRARLAARFGPAARALVPDGGLWVAWPRKGSGV
                     PTDLDFDAVQRVGLDDGLVDNKVAAIDQVWTSVRFVVRVADRPGRRAAGHRPGR"
     gene            complement(1271..1438)
                     /locus_tag="VEP73_03750"
     CDS             complement(1271..1438)
                     /locus_tag="VEP73_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25582.1"
                     /translation="MIPGAVLAMLSAIALLAVSLFRPDGLTFVWWSLAADAVAVTLLV
                     RALARRGAPPS"
     gene            complement(1476..>2140)
                     /locus_tag="VEP73_03755"
     CDS             complement(1476..>2140)
                     /locus_tag="VEP73_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25583.1"
                     /translation="ALVMAQRMADQTVEEAKSKAKAMVSEAEARSKNMTEQSQLRSRE
                     VTEAAQMRAREVTEAAQMRAREVTETAQARARELTEGLETRYKERIQSAEARARVSEE
                     QARMQIAQATEQVARRRQELESSIEALRAFERDYRARLRGFVEGQLKALEDAAPSGPV
                     APNPPPGLGGNNSRPLPGSDDLPALPTGARQVNNSYQTLREGDQLGHNGRDRVDRLRH
                     DD"
BASE COUNT          226 a          798 c          850 g          266 t
ORIGIN      
        1 ggccgtggac gaccgcatcg acctcgccgt caaggccgag ggcgaggtgg ccgaggcgct
       61 cgacgcccac cgcgaccacg tgctcggcga gaccctggcc acgtcgctgc accgcgcccc
      121 gcagggggac ggctacgacg cccgggtgga gctggacggg cagacggtgc gcctgtggct
      181 gcggccccgg ccccgggcgt aggcgcgcgg atgcgggcgc gcgacgacgg catcgggggg
      241 ctgctgcccg ccgcccggcc gtggtggggg tacctctggg cggcgccgaa cagcctggtc
      301 gggctggtcg gggcggcctc gtccggcggg aggccggtgc gctggcgggg cgtgctcctg
      361 tgcgagggcg gccgcgccgg gctcgcgagg ttcctcgcct ggcgcgggtt ctgcgcgatc
      421 accctggggc acgtgatcgt ggccagcgag cccctcgacg accacctcct cgcccacgag
      481 ctcggccacg tccgccagca cgagcgctgg gggccgctct tctacccggc ctacctgctc
      541 gcgagcctcg caggctaccg gcggaacccg ttcgagcgcg ccgccgagcg gcgcgcggtc
      601 cacgccctcg cccaccaggc cggaccggcg gcccactccc ccggcgagcc cggtgaccgg
      661 ccaggggtcg gtcgtgcctg accggcgcgg ggcgcggccg ggccacgccg actactcggg
      721 cacgccgctt ccacgcaagc tcggcatcag gcccggggcg acggtggcgc tcctggacgc
      781 gcccgagggc gccggcgacc tgctcgggcc tctgcccgac ggggtcgtcg tgcgccggcg
      841 cgcgcagggg ggcctcgacg tggtcgtgct gttcgtgacc tcgcgggcca ggctcgcggc
      901 ccgcttcggc ccggcggccc gcgcgctcgt ccccgacggc gggctctggg tggcctggcc
      961 caggaagggc tctggcgtgc ccaccgacct cgacttcgac gccgtccagc gggtcgggct
     1021 ggacgacggg ctggtcgaca acaaggtggc cgcgatcgac caggtgtgga ccagcgtgcg
     1081 cttcgtggtc agggtcgccg accggcccgg acggcgcgcg gccggccacc gccccggccg
     1141 ctgaccctgc cgccgagccg agcgccgacc ccggccgccg tcagcaggac gtgccgggtc
     1201 gggtcggggg cccgcccggc ccggcgaggg aaccgatcgc tcggcgccgg gacgggggcc
     1261 ggggcgcccg ctacgagggc ggggcgccgc ggcgggcgag tgcgcggacc agcagcgtta
     1321 cggcgacggc gtcggcggca agcgaccacc agacgaacgt cagcccgtcg ggccggaaca
     1381 gggagacggc gagcaacgcg atggcggaca gcatcgcgag cacggcaccc gggatcacgg
     1441 ccggacgtca ggcgggcagg atggcggctg ttcgttcagt cgtcgtggcg caggcggtcg
     1501 acccggtcgc ggccgttgtg gccgagctgg tcgccctccc ggagcgtctg gtaggagttg
     1561 ttgacctgcc gcgccccggt gggcagggcg gggaggtcgt cgctgccagg cagcggccgg
     1621 gagttgttgc ccccgagccc gggtggcggg ttcggggcca ccgggccgga cggggcggca
     1681 tcctccaagg ccttgagctg cccctccacg aagccgcgga ggcgggcacg gtagtcgcgc
     1741 tcgaacgcgc ggagcgcctc gatcgagctc tcgagctcct gccggcggcg ggccacctgc
     1801 tcggtcgcct gggcgatctg catgcgggcc tgctcctcgg acacgcgggc gcgcgcctcg
     1861 gcggactgga tgcgctcctt gtagcgggtc tcgaggccct cggtgagctc ccgcgcgcgg
     1921 gcctgcgcgg tctcggtgac ctcgcgggca cgcatctgcg ccgcctcggt gacctcgcgg
     1981 gcacgcatct gcgccgcctc ggtgacctcc cgggagcgca gctgggactg ctcggtcatg
     2041 ttcttcgagc gcgcctcggc ctccgacacc atcgccttcg ccttggactt ggcctcctcc
     2101 acggtctggt cggccatgcg ctgcgccatg acgagggccc
//
LOCUS       DASPYY010000247         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_466837, whole genome shotgun sequence.
ACCESSION   DASPYY010000247 DASPYY010000000
VERSION     DASPYY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466837"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VEP73_03760"
     CDS             <1..418
                     /locus_tag="VEP73_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYT25584.1"
                     /translation="FAFASAVAAIDGKDGALAGMADRVDGPLAASFTQLDLAVGRAYP
                     LASMLSRDATSGFEDLLYRWGGVGHDGFQAVDAAALTMGRVEQPYQFAPGRFFNLDAN
                     AVIKDGGPPSGSHSDIVHPEIAWALLSAAGIATGGA"
     gene            493..789
                     /locus_tag="VEP73_03765"
     CDS             493..789
                     /locus_tag="VEP73_03765"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF002571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HYT25585.1"
                     /translation="MPLVQITMLRGRTDAQKRALLEAVTEAVHTSIETPVESIRVWIS
                     EMEPIGYMAAGVLAADRPGAEPPAGAGHTPGAVAPGRPPEVLSQPAATDPDPSR"
     gene            complement(805..1215)
                     /locus_tag="VEP73_03770"
     CDS             complement(805..1215)
                     /locus_tag="VEP73_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25586.1"
                     /translation="MPSLSNDARTTAGILLLTIVAVEWGGTFMLRIVRGRQPMTKFQH
                     AFARAGHAHAGVLVTLSLVCQLFVDSADVHGLLGLLARSGVPFAAVLMPAGFFLSSAG
                     RGTTEPNRLIVLLWAGTVSLALGVVSLGVGLLAA"
     gene            1359..1907
                     /locus_tag="VEP73_03775"
     CDS             1359..1907
                     /locus_tag="VEP73_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYT25587.1"
                     /translation="MPGRPLNATAASLLGFLHEGPMTGWDLVATAEARIGDFWSLTQS
                     QVYRELAAMAEGGLVEAGERGPRDRQPYTITDAGRAAFAGWVDREPGPETIRFPLLLT
                     ILFGRHLPPERLAAFVRRHRAEHTDRLARYERLRDAAGDAAAAGDPYGTATLAFGLAY
                     ERAVLAWFDELPPAFRGDGGTG"
     gene            complement(1904..>2089)
                     /locus_tag="VEP73_03780"
     CDS             complement(1904..>2089)
                     /locus_tag="VEP73_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYT25588.1"
                     /translation="LVERRERWTVSLGLSQLSGSIETPTTILMAGAVILTIPIVVLFF
                     LTERLLVEGLTSGAEKG"
BASE COUNT          305 a          763 c          760 g          261 t
ORIGIN      
        1 cttcgcgttc gcgagcgcgg tcgcggcgat cgacgggaag gacggcgcgc tcgccggcat
       61 ggccgaccgg gtcgacggcc ccctggccgc ctccttcacc cagctcgacc tcgccgtcgg
      121 cagggcgtac ccgctcgcgt cgatgctgag ccgggacgcg acctccggct tcgaggacct
      181 gctgtaccgc tggggcgggg tcggccacga cgggttccag gcggtcgacg cggccgccct
      241 gacgatgggc cgggtcgagc agccgtacca gttcgcgccg gggcgcttct tcaacctcga
      301 cgccaacgcc gtcatcaagg atggcgggcc gccgtcgggc tcccactccg acatcgtcca
      361 tcccgagatc gcctgggcgc tgttgtcagc ggccggcatc gcgaccggcg gcgcctgacg
      421 cgggccccta tgatcggggc atgaacgagc gcccgagctc gacgccctcg gcaccgtgga
      481 ggtccatggc tgatgcccct cgtccagatc accatgctgc gcggacgcac cgacgcgcag
      541 aagcgcgccc tgctcgaggc ggtgaccgag gcggtccaca cctcgatcga gacgccggtc
      601 gagtcgatcc gcgtgtggat cagcgagatg gagccgatcg gctacatggc ggccggggtg
      661 ctggccgccg accggcccgg cgccgagccg cccgcgggcg ccgggcacac gcccggggcg
      721 gtcgccccgg gtcggcctcc cgaggtcctg tcgcagccgg cggccaccga ccccgacccc
      781 tcgcgctgac ggccgacttt cggttcacgc ggcgagcagg ccgaccccga gcgagacgac
      841 gccgagggcg agcgacaccg tccccgccca gagcagcacg atgagccggt tgggctcggt
      901 ggtcccccgt cccgccgacg agaggaagaa cccggcgggc atgaggacag ccgcgaacgg
      961 gacgccgctg cgcgccagga gcccgagcag gccgtgcacg tccgccgagt cgacgaagag
     1021 ctggcagacc agggacagcg tgaccagcac gcccgcgtgc gcgtgcccgg cccgggcgaa
     1081 ggcgtgctgg aacttcgtca tgggctggcg gccgcggacg atgcgcagca tgaacgtccc
     1141 accccactcg acggcgacga tcgtcagcag caggatgccc gcggtggtgc gggcgtcgtt
     1201 ggacagcgat ggcatgaggg cctccaggcc gatcccgcca cgggaacggg gttgcggtcg
     1261 acaccatgac ctcccacaat gatattccac aatggaaggt tctccccagg gtgggcctct
     1321 cacattggga tgtcaagcgc gtcgtgaaag gggaggccat gcccggccgg ccgctcaacg
     1381 ccaccgcggc atccctgctc ggcttcctgc acgaggggcc gatgacaggg tgggacctgg
     1441 tcgcgaccgc ggaggccagg atcggcgact tctggtcgct cacccagagc caggtctacc
     1501 gggagctcgc cgcgatggcc gagggcggcc tcgtcgaggc gggcgagcgc ggcccgcggg
     1561 accgccagcc gtacacgatc accgacgcgg gacgggcggc cttcgccggc tgggtcgacc
     1621 gcgagcctgg cccggagacg atccggttcc cgctgctgct cacgatcctg ttcggcaggc
     1681 acctcccgcc cgaacggctc gcggcgttcg tgcgacggca ccgcgccgag cataccgatc
     1741 ggctggccag gtacgagcgg ctgcgcgacg ccgcgggcga cgccgcggcg gcaggggacc
     1801 cgtatggcac cgcgaccctg gcgttcgggc tggcatacga gcgggcggtg ctcgcctggt
     1861 tcgacgagct ccccccggcc ttccgcggcg acggcgggac cggctagccc ttctccgccc
     1921 cgctcgtgag gccctcgacg agcagccgct cggtcaggaa gaacagcacg acgatcggga
     1981 tggtcaggat gaccgcccct gccatcagga tcgtggtcgg cgtctcgatg ctgccggaga
     2041 gctgggagag gcccagcgag acggtccagc gctcccgccg ctccaccag
//
LOCUS       DASPYY010000248         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_466904, whole genome shotgun sequence.
ACCESSION   DASPYY010000248 DASPYY010000000
VERSION     DASPYY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466904"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VEP73_03785"
     CDS             <1..192
                     /locus_tag="VEP73_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25589.1"
                     /translation="TPEALIGVVGSLVDERRRAELRAHSERLLRQHRPEVQREALDRV
                     YAAALRGGALRGGDPGAVR"
     gene            complement(161..1249)
                     /locus_tag="VEP73_03790"
     CDS             complement(161..1249)
                     /locus_tag="VEP73_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT25590.1"
                     /translation="MRLLLVVAALRHGGAERIVVQLAADAVRRGDAVTVASAGGPWVE
                     RVRAAGAGHELVPLDPRSAAVTLRSAGRLAAVVRRFRPEVVHAQNVRATLAAALALAR
                     PGRRPALVTTLHGLAPADYPAAARLLRLAGGTVIACAPAVGRSLLAAGFPAGRLEVIT
                     NGAALASPSEPELAAVRRRFAVGSRPLVVGIGRLAAQKSWPTLIEAASSIRDADVLVA
                     GDGPLRAELEAAAAAAGGRVRFVGPVERPAALVGLARCVVSTSAWEGLPLALLEALGL
                     GAPVVATAVDGVTDVVPRGAALLVPPGDPAAVAAAVNRVLTEPGLAGRLSRAARGASA
                     AWAPDAMLARYRERYAACVTAPRRDRRP"
     gene            1536..2900
                     /locus_tag="VEP73_03795"
     CDS             1536..2900
                     /locus_tag="VEP73_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HYT25591.1"
                     /translation="MSRTETKRKTVQERGAKPPRPARLAIPVADAAALLAAAAVFGRG
                     SALGPVYGVAAFLALAAAGTQWTRVNPTLGNDLPVLLGRLAVPFAPLAGLAVLGRVEA
                     ASLPDLVRVMAAAAVLVPAGRAVAYAVMRSARMRGHLLERVLIVGAGEVGGQLARVLQ
                     EHREYGLVPVGFVDSLDGSGLSMPVLGDARALAPTVERLQVGIVIVAFGAICEQELAG
                     VLRACDALPVEVYVVPRFFQFGVVPDSPSVGDVWGIPLVRLRRLALGRSARLAKRAFD
                     LAGAGAGLLLSAPLFAAAALAVRRSSPGPVFFRQLRVGKDGRPFELVKFRTMRVNDDS
                     DTTWSVAGDTRVTRAGRLLRRTHLDELPQLLNVLRGQMSLVGPRPERPHFAHRFAGEV
                     PRYGDRHRVRGGITGWAQVHGLKGDTPIPDRARFDNHYIEHWSLWRDVVIVTRTLANV
                     LLGR"
     gene            3081..>3484
                     /locus_tag="VEP73_03800"
     CDS             3081..>3484
                     /locus_tag="VEP73_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25592.1"
                     /translation="MAKRGLWLLAVLSVAPAGVLAAWLVEGWGGGFATRSADDIGLLG
                     FAVFGAVCSFLAARRSSGRQRATWLAMAGGLGAWSAGEVVWCYYELWRGLPQTPFPSP
                     ADAAYLLFPVGRPCVDALPRRSRRSVPVPAGPR"
BASE COUNT          379 a         1349 c         1313 g          443 t
ORIGIN      
        1 acgccggagg cgctgatcgg cgtggtcggc tcgctcgtcg acgagcgccg ccgcgcggag
       61 ctgcgcgcgc actccgagcg cctgctccgc cagcaccgcc cggaggtcca gcgggaggcg
      121 ctcgatcgcg tgtacgcggc ggcgctgcgc ggcggcgccc tcaggggcgg cgatcccggc
      181 gcggtgcggt gacgcaggcc gcgtagcgtt cccggtagcg cgcgagcatg gcgtccggcg
      241 cccatgccgc cgacgcgccg cgcgccgcgc gcgagaggcg cccggccagg cccggctcgg
      301 tgagcacgcg gttgacggcg gcggcgaccg cggcagggtc gccgggcggg accagcaggg
      361 cggcgccccg ggggacgacg tccgtgacgc cgtcgaccgc cgtcgcgacc accggggcgc
      421 cgagcccgag cgcctcgagc agcgccagcg gcaggccctc ccaggccgag gtggacacga
      481 cgcagcgggc caggccgacg agggccgccg gccgctcgac cgggccgacg aaccgcaccc
      541 ggccgcccgc cgcggcggcc gccgcctcca gctcggcccg cagcggaccg tcgcccgcga
      601 ccagcacgtc ggcgtcccgg atggagctcg cggcctcgat cagggtcggc caggacttct
      661 gtgcggccag ccggccgatc ccgaccacca gcggccggct gccgaccgcg aaccgccgcc
      721 gcaccgccgc cagctcgggc tcggagggtg acgccagggc ggcgccgttg gtgatgacct
      781 cgagccggcc ggcggggaac ccggcggcca ggagcgagcg gccgaccgcc ggggcgcagg
      841 cgatcaccgt gcccccggcg agccgcagca ggcgggcggc ggcgggatag tcggccgggg
      901 cgagcccgtg caaggtggtg acgagcgccg gccgcctgcc cgggcgggcg agcgccagcg
      961 ccgctgccag cgtcgcccgc acgttctgcg cgtgcacgac ctcgggacgg aagcggcgca
     1021 ccaccgcggc gagccggccg gccgacctca gcgtgacggc cgccgagcgc gggtcgagcg
     1081 ggacgagctc gtggccggcc ccggccgccc gcacccgctc gacccagggg ccgccggccg
     1141 acgccaccgt caccgcgtcg ccgcgccgga cggcgtcggc cgcgagctgc acgacgatgc
     1201 gctcggcgcc gccatggcgc agcgccgcga cgacgaggag cagcctcatg gccaccggcc
     1261 cgacgacttg tccgaacttg gcgttgttgg tggtggttga atgggtactc cggcatggtg
     1321 gatcgatacc attcggcgcc cgtgcccaca gggggggagt tgtgaccttt catcctcggt
     1381 gacgttcgtg gccgggcccg cgccggcgcg gcctcccgcg ctcgcgcgac cccgcgcacg
     1441 cgacgggcgg cccgggccca tggccgggtt cccgccacga acggcacaga tcgtcatggc
     1501 gaccgagtcc tctgtgttcg caccgagagc agactatgtc ccgcaccgag accaagagga
     1561 agaccgtcca ggagcgcggc gccaagccgc cgcgaccggc gaggctcgcc atccccgtgg
     1621 cagacgcggc cgcgctcctc gccgccgccg ccgtcttcgg gcgcgggagc gcgctgggtc
     1681 cggtctacgg cgtcgccgcc ttcctcgcgc tcgcggccgc cggcacgcag tggacacggg
     1741 tcaacccgac gctcggcaac gacctccccg tgctgctggg ccggctcgcc gtgccgttcg
     1801 cgccgctcgc cggtctcgcc gtgctgggga gggtggaggc ggcctcgctc ccggacctgg
     1861 tccgtgtcat ggcggcggcg gccgtcctgg tcccggcggg gcgggcggtc gcctacgcgg
     1921 tgatgcgttc cgcccggatg cgagggcacc tgctcgagcg ggtcctgatc gtcggcgcgg
     1981 gcgaggtcgg cggccagctg gcgagggtcc tccaggagca ccgcgagtac ggcctggtgc
     2041 ccgtcgggtt cgtcgactcc ctcgacggca gcgggctgtc gatgccggtc ctcggcgacg
     2101 cgcgggcgct cgcccccacg gtcgagcgcc tgcaggtggg gatcgtcatc gtcgccttcg
     2161 gcgcgatctg cgagcaggag ctcgccggcg tcctgcgcgc ctgcgacgcg ctgccggtcg
     2221 aggtgtacgt cgtgccgcgg ttcttccagt tcggggtcgt ccccgacagc ccctcggttg
     2281 gcgacgtctg gggcatcccc ctggtccggc tgcggcggct ggcgctcggg cgcagcgccc
     2341 gcctggccaa gcgcgccttc gacctcgccg gcgccggcgc cggcctgctg ctctcggcgc
     2401 ccctgttcgc cgccgccgcc ctggcggtgc ggcgctccag ccccggcccg gtcttcttcc
     2461 ggcagctgcg ggtcggcaag gacgggcgcc ccttcgagct ggtcaagttc cggaccatgc
     2521 gggtcaacga cgactccgac acgacctggt cggtggccgg cgacacgcgc gtgacccggg
     2581 ccggccggct gctgcggcgc acgcacctgg acgagctgcc ccagctcctg aacgtcctgc
     2641 gggggcagat gtcgctggtg ggcccgcgtc ccgagcggcc ccacttcgcc caccgcttcg
     2701 ccggcgaggt gccgcgctac ggcgaccggc accgggtccg cggcggcatc accggctggg
     2761 cccaggtcca cgggctcaag ggggacacgc ccatccccga ccgggcccgc ttcgacaacc
     2821 actacatcga gcactggtcg ctgtggcgcg acgtcgtgat cgtcacgcgc accctcgcca
     2881 acgtcctcct cggcaggtga ccccggcagg tgatggcgcc tcaccgcacc gactgccagg
     2941 ccgcagctca tccaccagcc cgcgcaacag ccacagcccc ctcccgccaa gcgcccagct
     3001 cgccggtgag ggtgcttgcc gctcccacct cacgctgtag gccggccgcg atctgccgat
     3061 ctccctgtca ggaggtccgg atggcgaagc gcggattgtg gctgctcgcc gtgttgagcg
     3121 tggcgccggc aggcgtgctt gcggcgtggc tggtggaggg atggggcggg gggttcgcca
     3181 cccggtccgc agacgacatc ggcctgctgg gcttcgccgt cttcggcgcg gtctgctcct
     3241 tcttggcggc tcgccgcagc agtggtcgtc agcgagccac ctggctggcg atggccggcg
     3301 gtctgggcgc ctggtcggcc ggcgaggtcg tgtggtgcta ctacgagctg tggcgaggcc
     3361 tgccccagac cccgttccca tcgccggccg acgccgccta cctcctgttc ccggtcggcc
     3421 gcccttgcgt tgacgctctt ccccggcggt cgcgtcgctc agtcccggtt ccggctggtc
     3481 ctcg
//
LOCUS       DASPYY010000249         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_325115, whole genome shotgun sequence.
ACCESSION   DASPYY010000249 DASPYY010000000
VERSION     DASPYY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325115"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VEP73_03805"
     CDS             <1..371
                     /locus_tag="VEP73_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYT25593.1"
                     /translation="TGHPLARATMPPARRRGIAALAGVFKVPVVEDGTRVDPRPVPGT
                     PPPLLAAFARGAPVLSLGGMGEPFWPELRVGWIRAPAGTAEALAHLQAEAGASARPAA
                     QFLAAKLLAHPPPLEVRTIM"
     gene            465..959
                     /locus_tag="VEP73_03810"
     CDS             465..959
                     /locus_tag="VEP73_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25594.1"
                     /translation="MLFWIAWYLPLVGLWLLLVATLARAELLLGLVAAAVGATAQELV
                     NAQDLVRFRLRARWLRDLRLLPRQVLVDSGRLAVVLWRQLVRGEPATGTFRTVPFPAA
                     GPVGGGDAEENARRALVTTAVSLSPGTYVVGIEGGEGVMVVHQLPAGSGSLVPPSMAG
                     RVPE"
     gene            956..1339
                     /locus_tag="VEP73_03815"
     CDS             956..1339
                     /locus_tag="VEP73_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HYT25595.1"
                     /translation="MNLSQWLVASVALLAGLVPCAVVCLRGDACSRLVGLEMGATVDT
                     LVLLLLAEAYGRVIYFDLAVALALLSFAGGMVFAVDQAAPPSPPSAGCSRPRRSSSPG
                     ATSRPSGPSSTRSCSSSLPAWWGSA"
     gene            1500..>2320
                     /locus_tag="VEP73_03820"
     CDS             1500..>2320
                     /locus_tag="VEP73_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYT25596.1"
                     /translation="MLYGRSGALNLAQIGRTLAGRPVDRLVVVAFTLLLAGFFVKAAV
                     VPFHFWLADAYALAPTPVCILLAGVVSELGVYAATRVYWTAFSGALAPHAPGLRGVLL
                     GAAVLTALVGAVMCFLQHHLARLLAFATVSHVGLVLAGVALLAPGGTGGAFLYLVADG
                     LIKASLFVCVGIVQHRLASVDEHALHGRGRGLPWTFGLMVAGALGLAGTTPFGTFLGK
                     ALMEHAAGAAGQHWLTWVFVATSALTAAAVLRAALRVFAGWGPPAEAGGHDPVAAA"
BASE COUNT          198 a          889 c          862 g          371 t
ORIGIN      
        1 cgaccgggca cccgctggcg cgggcgacga tgccgccagc gcgccgccgc gggatcgccg
       61 ccctcgccgg ggtgttcaag gtccccgtcg tcgaggacgg gacgcgggtc gaccctcgcc
      121 cggtcccggg cacccctccc ccactgctcg ccgccttcgc ccggggcgcc ccggtgctca
      181 gcctcggggg catgggggag ccgttctggc cggagctgcg ggtcggctgg atccgggcgc
      241 cggccgggac cgcggaggcg ctcgcccacc tccaggccga ggcgggcgcg tcggcgcggc
      301 cggccgccca gttcctggcc gccaagctgc tcgcccatcc gcccccgctt gaggtgcgga
      361 cgatcatgtg aaaccttcgc gccgacccgc cgtcgcaggg cctcggcccg acggtcggcg
      421 agccggccgg accctttcga cacggggggc ggatgaggaa gcggttgctc ttctggatcg
      481 cctggtatct gccgctcgtc gggctctggc tgctgctcgt cgccaccctc gcccgcgccg
      541 agctgctgct cgggctcgtc gccgcggcgg tcggagccac cgcgcaggag ctggtgaacg
      601 cccaggacct cgtccgcttc cggctgcggg cgcgctggct gcgcgacctc cgcctcctgc
      661 ccaggcaggt gctggtggac tcggggcgcc tcgcggtcgt cctgtggcgg cagctcgtgc
      721 gcggcgagcc ggcgaccggc acgttccgga ccgtcccctt cccggcggcg ggcccggtgg
      781 gcggcgggga cgccgaggag aacgcacggc gggcgctcgt gaccaccgcg gtctcgctct
      841 cgcccggcac ctacgtggtc ggcatcgagg gaggcgaagg cgtcatggtc gtccaccagc
      901 tccccgccgg ctcgggctcg ctggtgcccc ccagcatggc gggccgggtg ccggagtgaa
      961 cctgagccag tggctcgtgg cgagcgtcgc gctgctcgcc gggctcgtgc cctgcgcggt
     1021 cgtgtgcctg cggggcgacg cctgctcgcg cctggtcggc ctggagatgg gcgcgacggt
     1081 cgacacgctc gtgctcctgc tgctcgcgga ggcctacggc cgggtgatct acttcgacct
     1141 ggccgtcgcg ctcgccctcc tgtccttcgc gggcgggatg gtgttcgcgg tcgaccaggc
     1201 cgcgccaccc tcgccaccct cggcgggctg ctcgcgaccg cggcgctcgt cttctcctgg
     1261 cgctacttcg aggccgtcgg gaccctcttc cacgcgctca tgctcgtctt ccttgccggc
     1321 gtggtggggt tctgcctgag cggcgacctg ttcaacctgt tcgtgttctt cgagctcctg
     1381 agcgtctccg gctacgccct cgccggctac cggatcgagg acctgggcgc gctccagggg
     1441 ggctcaactt cgcggtcacc aacagcgtcg gcgcgttcct cgtcctgtcc ggcatcgcgc
     1501 tgctctacgg ccgcagcggg gcgctcaacc tggcccagat cgggcggacg ctcgcgggga
     1561 ggccggtcga ccgcctggtg gtggtcgcgt tcacgctcct gctggccggc ttcttcgtga
     1621 aggcggccgt ggtgccgttc cacttctggc tcgccgacgc ctacgccctg gcgcccacgc
     1681 cggtctgcat cctgctcgcc ggcgtggtca gcgagctcgg ggtctacgcc gcgacccgcg
     1741 tctactggac cgcgttctcg ggcgcgctcg ccccccacgc gcccggcctg cgcggggtgc
     1801 tgctcggggc ggcggtgctg accgcgctcg tcggcgcggt catgtgcttc ctgcagcacc
     1861 acctcgcccg gctgctcgcc ttcgccaccg tcagccacgt cggcctggtc ctggccggcg
     1921 tcgcgctgct cgcgccgggc ggcacgggcg gcgcgttcct ctacctggtg gccgacgggc
     1981 tgatcaaggc gtcgctgttc gtctgcgtcg ggatcgtcca gcaccggctg gccagcgtgg
     2041 acgagcatgc gctgcacggg cggggccgcg gcctgccctg gacgttcggc ctgatggtgg
     2101 ccggcgccct cggcctcgcc gggacgacgc cgttcggcac cttcctcggc aaggcgctca
     2161 tggagcacgc ggccggcgcg gccgggcagc actggctgac ctgggtgttc gtcgccacct
     2221 cggccctgac cgccgccgcg gtgctgcgtg cggccctgcg ggtgttcgcc ggctgggggc
     2281 cgccggccga ggccggcggc cacgacccgg tggcggcggc
//
LOCUS       DASPYY010000250         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2713704, whole genome shotgun sequence.
ACCESSION   DASPYY010000250 DASPYY010000000
VERSION     DASPYY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713704"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1751
                     /locus_tag="VEP73_03825"
     CDS             <1..>1751
                     /locus_tag="VEP73_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYT25597.1"
                     /translation="LAAAAAGPVLGSALVVVLGSALVVAGAVAAPAASATLYVDRSSP
                     SCSGSGPGSATAPYCSIARAAAAATAGQTVLVRPGSYPESVTVGHSGTAAAPIVLRSL
                     GGVTVRGGSHGFTVSSRSWVTVQGFTVTGTAGAGIYVKGSSHVAIVGNHVTRSGRPSS
                     GDTAQGIRLESTSDSRVAGNTTDHNSEAGILLAGGSTRNRVAGNESFANARGYTRAAP
                     GIDVRAPGNSVTGNRSHDNEDTGIQSYTGGDNTLVANNVAWSNGDHGIDDLGVTGQRI
                     VGNTVYGNVASGINLEGASSGATVENNVSVDNGIGSPRTKGNIRVDSTSTSGATVDYN
                     LVSLGASAVMYTWGTTGYPSLAAFRSATGQEPHGIQADPRFAGARDFHLLAGSPAVDS
                     ANSGQSGQQAVDAEGRPRFDDPGVANTGAGPRAFDDRGAFERQSGSQGTGGGGNLVAN
                     PGFERDLTGWTGTGIARVPGGRTGFAARLGNPGSAAASCTLNDSPNWVARTSAGSYTG
                     SLWVRADAAGAGLKLRLREYSGQALVKTSTTTATLGTAWQQVTVVHAVSSAGTTLDLN
                     AYVSSTPPGTCFYADDA"
BASE COUNT          248 a          686 c          621 g          196 t
ORIGIN      
        1 ggctcgccgc cgccgcggcc ggcccggtcc tcggctcggc cctggtcgtc gtcctcggct
       61 cggccctggt cgtcgccggg gcggtcgccg ccccggccgc aagcgccacc ctctacgtcg
      121 accggtccag cccgagctgc tcgggctcgg gaccaggcag cgccaccgcg ccgtactgct
      181 cgatcgccag ggcggccgcg gccgcgacgg ccgggcagac cgtgctggtg cggccgggca
      241 gctatcccga gagcgtcacc gtcgggcact cgggcaccgc cgccgcgccg atcgtcctca
      301 ggtcgctcgg cggggtgacc gtacgcggcg ggtcgcacgg gttcacggtc tcctccagga
      361 gctgggtcac cgtgcagggc ttcacggtga ccgggacggc cggggccggg atctacgtga
      421 agggctcgag ccacgtcgcc atcgtcggca accacgtgac gcggtccggc cgcccgtcct
      481 cgggcgacac ggcgcagggc atccggctcg agtcgaccag cgactcgcgg gtcgcgggca
      541 acacgaccga ccacaacagc gaggccggga tcctgctcgc cggcggctcg acccggaacc
      601 gggtcgccgg caacgagtcg ttcgccaacg cccgcggcta cacccgcgcc gcgccgggca
      661 tcgacgtgcg cgccccgggc aactcggtga ccggcaaccg gtcccacgac aacgaggaca
      721 ccggcatcca gtcctacacc ggcggcgaca acaccctcgt cgccaacaac gtggcctgga
      781 gcaacggcga ccacggcatc gacgacctgg gcgtcaccgg ccagcggatc gtcggcaaca
      841 ccgtgtacgg caacgtggcg tcggggatca acctggaggg cgcctcctcg ggcgcgaccg
      901 tcgagaacaa cgtctcggtc gacaacggga tcggctcgcc gcgcaccaag ggcaacatcc
      961 gggtggactc gacctccacc tccggggcga cggtcgacta caacctcgtc tcgctcggcg
     1021 cctccgccgt gatgtacacg tggggcacga ccggctaccc gtccctggcc gcgttcagga
     1081 gcgcgaccgg gcaggagccg cacggcatcc aggccgaccc gcggttcgcg ggcgcccgcg
     1141 acttccacct gctggccggg tcccccgccg tcgactcggc caactcggga cagagcgggc
     1201 agcaggccgt cgacgccgag ggcaggccga ggttcgacga cccgggcgtg gccaacaccg
     1261 gcgccggccc gcgcgccttc gacgaccgcg gcgcgttcga gcgccagagc gggtcgcagg
     1321 gcaccggtgg cgggggcaac ctggtcgcca accccggctt cgagcgcgac ctcaccggct
     1381 ggaccggcac cggcatcgcg cgggtcccgg gcggccgcac cggcttcgcg gcccggctcg
     1441 gcaacccggg ctcggcggcg gcgagctgca ccttgaacga ctcgcccaac tgggtggcga
     1501 ggacgtccgc cggcagctac accggcagcc tgtgggtgcg ggcggacgcc gccggggcgg
     1561 gcctcaagct ccgcctgcgc gagtacagcg gccaggcgct ggtcaagacg agcacgacca
     1621 ccgcgaccct cggcaccgcc tggcagcagg tcacggtcgt ccacgccgtg agctccgcgg
     1681 gcacgaccct cgacctcaac gcctacgtct cgagcactcc gcccgggacc tgcttctacg
     1741 ccgacgacgc g
//
LOCUS       DASPYY010000251         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_44317, whole genome shotgun sequence.
ACCESSION   DASPYY010000251 DASPYY010000000
VERSION     DASPYY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44317"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VEP73_03830"
     CDS             <1..640
                     /locus_tag="VEP73_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYT25598.1"
                     /translation="RRPGHGILGEEHGASGDQRTRWVIDPVDATCNFIRGIDWWATLL
                     ALEADGQAQVAMVSAPALGRRWWAVRGQGAFTTGPLDPRPRPLAVSRVHELGDAQLAY
                     GDLRGDPWFQTLAARCWRTRGIGDFLAWCLLADGAVDVVVGQDTDRRWDLAAPILLVE
                     EAGGRVTGPAGQPWTGSRQPAIGSNRLLHPAVVDLIGANVAARHTAAPDRGG"
     gene            738..2300
                     /locus_tag="VEP73_03835"
     CDS             738..2300
                     /locus_tag="VEP73_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYT25599.1"
                     /translation="MRHRPTTTTPTSTPEPEDGLLRRVGYWPQFARTLGWGESFGLAF
                     SFISVTTGIFTTFGFLLATAGPRGIWTWPVAVGGQTLVALVYATLAAQMPLAGGAYQW
                     ATRLAGPLAGWWLGWLSLSFLVIVTVSVDYALIQAAVWPLLGLPATPATLAAGTLAVI
                     ATQAALLCWSTPATARLNNLAVLAEIIGLLQLTIVLLVAGRIAGIGRWANLTSTAPIP
                     AHGWWAWLGPGMLATLLGAYTIVGFEASATLAEETRQPARVVPRAMVQAVIASGVLGM
                     LFLVALAQAIPHPDRIAGDAAPVAAILRDFLGGRIEPFLAFICLAIYTCGTVIMTTAS
                     RLAFAMARDRRLPAHQLLAHVPRATGGPVYAVGAVAGASAAIVATLGANPDALTTLFT
                     ASTLLPAILYTGTVALAVWAARHAPPGTEAASFTLGAWQRPVTVGALAWLGYELLVLL
                     APARFRPAQAYALGAVALGALIYLLLRLAEPDAMTRQPASVPVDPDTHPAPDPEARGG
                     QPREPRRRLLRQ"
     gene            2304..>2674
                     /locus_tag="VEP73_03840"
     CDS             2304..>2674
                     /locus_tag="VEP73_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYT25600.1"
                     /translation="MSTTPDDTPTDAELLAAVAEGDPEAFGALYDRHAPWLLLRLRQR
                     AASSDLADQVAQETFLVVWRDARRFRGRGEVGAWIWGIAIRRLLDLQRGDAAQGRLAR
                     LLGRRRVPDAASAEEQALTTL"
BASE COUNT          295 a         1156 c          869 g          354 t
ORIGIN      
        1 ccgccgcccc ggccacggca tcctcggcga ggagcacggc gccagcggcg accagcgcac
       61 ccgctgggtc atcgacccgg tcgacgcgac ctgcaacttc atccgcggga tcgactggtg
      121 ggcgaccctg ctcgcgttgg aggccgacgg gcaggcccag gtggcgatgg tgtcggcgcc
      181 ggcgctgggg cggcgctggt gggccgtgcg cgggcagggg gcgttcacga ccgggccgct
      241 tgacccgcgc ccccgcccgc tggccgtctc ccgcgtccac gagctcgggg acgcgcagct
      301 ggcctacggc gacctccgcg gtgacccctg gttccagacg ctcgccgccc gctgctggcg
      361 gacccggggg atcggcgact tcctcgcgtg gtgcctgctg gccgacggcg ccgtcgacgt
      421 cgtcgtcggc caggacaccg accggcgctg ggacctcgcc gcccccattc tgctcgtcga
      481 ggaggccggc gggcgcgtca ccggcccggc cgggcagccc tggaccggca gccgccagcc
      541 cgccatcggc agcaaccgcc tgctccaccc cgccgtcgtg gacctcatcg gcgccaacgt
      601 cgccgcccgg cataccgcag ccccggaccg cggcggctga cccggcgccg acttgctcat
      661 cccgctgcgg ccagcgttcg ggctggcctg cccgacgcgt cccacccacc cacccaccca
      721 cccacccagg agacccgatg cgccaccgcc ccaccaccac gacacccacc agcacccccg
      781 agcccgagga cgggctcctg cgccgggtcg gctactggcc ccagttcgcc cgcaccctcg
      841 gctggggcga gtcgttcggg ctggcgttct cgttcatctc cgtcaccacc ggcatcttca
      901 ccaccttcgg gttcctgctg gccaccgccg gcccccgcgg gatctggacc tggccggtgg
      961 cggtgggcgg ccagaccctc gtcgccctcg tctacgccac cctcgccgca cagatgcccc
     1021 tggccggcgg cgcctaccag tgggccaccc gcctcgccgg ccccctggcc ggctggtggc
     1081 ttggctggct gtcgctttcg ttcctggtga tcgtcaccgt ctcggtcgac tacgccctca
     1141 tccaggctgc cgtctggccg ctcctcggcc tgcccgcgac ccccgccacc ctcgccgccg
     1201 gcaccctggc ggtcatcgcc acccaggcag cgctgctgtg ctggtccacc ccggccaccg
     1261 cccggctcaa caacctcgcc gtcctcgccg agatcatcgg cctcctccag ctcaccatcg
     1321 tcctgctggt cgccggccgc atcgccggca tcggccgctg ggccaacctc accagcaccg
     1381 cccccatccc ggcccacggc tggtgggcgt ggctcggccc cgggatgctc gccaccctgc
     1441 tgggcgccta caccatcgtc ggcttcgagg catccgccac cctcgccgaa gagacccgcc
     1501 agcccgcacg ggtcgtcccc cgcgccatgg tccaggcggt catcgcctcc ggggtgctcg
     1561 gcatgctgtt cctcgtcgcg ctcgcgcagg cgatccccca ccccgaccgc atcgcgggcg
     1621 acgccgcgcc ggtcgcggcc atcctgcgcg acttcctcgg cggccgcatc gaacccttcc
     1681 tggcgttcat ctgcctggcg atctacacct gcgggaccgt gatcatgacc accgcctccc
     1741 gcctcgcctt cgcgatggcc cgcgaccggc ggctgccagc ccaccagctc ctcgcccacg
     1801 tccccagggc gaccggcggg ccggtctacg ccgtgggagc ggtcgccggc gccagcgccg
     1861 cgatcgtcgc caccctgggc gccaaccccg acgcgctcac caccctgttc accgcctcca
     1921 ccctgctccc cgccatcctc tacacgggca cggtcgccct cgccgtctgg gcggcccgcc
     1981 acgccccacc cggcaccgag gcggcgtcgt tcaccctcgg cgcctggcag cgccccgtga
     2041 ccgtcggcgc gctggcctgg ctcggctacg agctgctggt cctgctcgcc cccgcccgct
     2101 tccgccccgc ccaggcctac gcgctcggcg cggtcgccct cggcgcgctc atctacctgc
     2161 tcctgcgact ggccgaaccc gacgccatga cccgccagcc agcgtccgtc ccagtcgacc
     2221 ccgacaccca cccggcacca gaccccgagg cacggggcgg acagccccgt gaaccgcggc
     2281 gccggctgct gcgacagtga ggcatgagca cgaccccgga cgacaccccg accgacgccg
     2341 agctgctggc ggcggtggcc gagggcgacc ccgaggcgtt cggggccctg tacgaccggc
     2401 acgccccctg gctgctgctc cggctgcgcc agcgcgccgc cagctccgat ctggccgacc
     2461 aggtcgccca ggagacgttc ctggtggtgt ggcgggacgc ccggcgtttc cgcggccggg
     2521 gcgaggtggg cgcgtggatc tgggggatcg cgatccggcg cctgctcgac ctgcagcgtg
     2581 gcgacgccgc ccagggacgg ctggcgcggc tgctcggcag gcggcgggtg ccggatgccg
     2641 cgagtgccga ggagcaggcc ctcaccacgc tcga
//
LOCUS       DASPYY010000252         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_185994, whole genome shotgun sequence.
ACCESSION   DASPYY010000252 DASPYY010000000
VERSION     DASPYY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185994"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1306)
                     /locus_tag="VEP73_03845"
     CDS             complement(56..1306)
                     /locus_tag="VEP73_03845"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYT25601.1"
                     /translation="MTCDLVLAYSGGLDTTVAVHRLTHEQGRRVVALLADVGQGEDLP
                     ALARRALAAGAVDAVVVDARERFAAEFLTPAIAANALYEGRYPLVSALSRPLIAQLLV
                     EEAAARGAGAVAHGCTGKGNDQVRFEVSVGALAPHLEVLAPIRDWTMSRPEAIAYAEK
                     HGLDLGWITAAKPYSVDQNLWGRTVEAGVLEDPWTAPSEDAFAWTASPASGIWASPAS
                     GVGASPTAGAVAVAEAVVGFEAGVPTGIDGSAAGLTKVVAHLNRLGGAAGFGRLDMVE
                     NRRVGIKSREVYECPGALALILAHADLEGVCLERDVMREKGRLESRFAELVYDGLWHS
                     PLRRALDAFMVDTQEHVTGEVRLALDAGRCYVSGRRAPKGLYDHGLATYDASDTFRHA
                     DAEGFVRLWGLSVETWARRQGGAG"
     gene            complement(1312..1851)
                     /gene="argR"
                     /locus_tag="VEP73_03850"
     CDS             complement(1312..1851)
                     /gene="argR"
                     /locus_tag="VEP73_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HYT25602.1"
                     /translation="MPLKAERQAALAALLRTRQVTSQGGLLDELHRRGFDATQATVSR
                     DLEELGAVKVRGDGGRLVYALPEPVEAAGVSAEELRSVLALSLLAIVPTGNLVVVRTP
                     PGHANVLASTLDRAGLSGVAGTVAGDDTVLVVCADRTSGKAMARQLSALVAQPGSGPP
                     PLVDELGRPELTLYQGETA"
     gene            complement(1842..>2153)
                     /locus_tag="VEP73_03855"
     CDS             complement(1842..>2153)
                     /locus_tag="VEP73_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25603.1"
                     /translation="ADLGPLGQAAFTADHRRDADEFVRRHLTRLRELVERAGQPARHA
                     LAPTADTDAALAALGRGLVVNPVTATALRLAPPLVVTVEEVDEAIARLAGALEEVGAC
                     R"
BASE COUNT          273 a          799 c          827 g          254 t
ORIGIN      
        1 ccgaagcggc cccgccacag tgggcgcccc cggcgggcgt cgggttcctg atccgtcacc
       61 ccgcccctcc ctggcgccgg gcccaggtct ccaccgacag tccccacagg cggacgaagc
      121 cctcggcgtc ggcgtggcgg aaggtgtcgg aagcgtcgta ggtggcgagc ccgtggtcgt
      181 agaggccttt cggtgcgcgg cgccccgaca cgtaacagcg gccggcgtcg agggccaggc
      241 gcacctcgcc ggtcacgtgc tcctgggtgt cgaccatgaa cgcgtcgagg gcccggcgca
      301 gcggcgagtg ccacagcccg tcgtagacga gctcggcgaa gcgcgactcc agccggccct
      361 tctcccgcat gacgtcgcgc tccaggcaga cgccctccag gtcggcgtgg gccaggatca
      421 gggccagggc gccggggcac tcgtagacct cccggctctt gatgccgacg cgccggttct
      481 cgaccatgtc gagccgcccg aagcccgcgg caccgcccag ccggttcagg tgggcgacca
      541 ccttggtgag cccggccgcc gacccgtcga tcccggtcgg cacgccggcc tcgaacccga
      601 ccaccgcctc ggcgaccgcc acagcgcccg cggtgggcga ggccccgacg cctgaagcgg
      661 gcgaggccca gatgcctgaa gcgggcgagg ccgtccaggc gaaggcgtcc tcggagggcg
      721 cggtccacgg gtcctcgagc acgcccgcct cgaccgtgcg gccccagagg ttctggtcga
      781 ccgagtacgg cttggccgcc gtgatccagc cgaggtcgag cccgtgcttc tcggcgtacg
      841 cgatcgcctc cgggcggctc atcgtccagt cccggatcgg ggcgagcacc tccaggtggg
      901 gagcgagcgc cccgacgctc acctcgaagc ggacctggtc gttgcccttg ccggtgcagc
      961 cgtgggcgac cgcgccggcg cccctcgcgg ccgcctcctc gaccaggagc tgggcgatga
     1021 gcggccggga cagggccgag acgagcgggt acctgccctc gtagagcgcg ttggccgcga
     1081 tcgcgggggt gaggaactcg gccgcgaagc gctcgcgcgc gtcgacgacg accgcgtcga
     1141 ccgccccggc ggccagcgcg cgccgcgcga gcgcgggcag gtcctcgccc tggccgacgt
     1201 cggcgagcaa cgcaacgacc cgtcgcccct gctcgtgggt gagccggtgg acggccacgg
     1261 tggtgtcgag gccgcccgag taggcgagga cgaggtcgca ggtcacacgg gtcatgcggt
     1321 ctctccttgg tagagggtga gctcggggcg gccgagctcg tcgacgaggg gtggcggccc
     1381 gctcccgggc tgggcgacga gcgccgagag ctgccgcgcc atcgccttgc ccgaggtgcg
     1441 gtcggcgcag acgacgagga cggtgtcgtc gcccgcgacc gtgccggcca cgcccgacag
     1501 gcccgcgcgg tccagcgtcg aggccagcac gttggcgtgg ccgggcgggg tgcgcacgac
     1561 cacgaggttg ccggtgggga cgatcgcgag cagcgagagg gcgaggacgc tgcgcagctc
     1621 ctccgcgctc accccggccg cctcgaccgg ctcggggagc gcgtagacga ggcgcccgcc
     1681 gtcgccgcgc accttgaccg cgcccagctc ctcgaggtcg cgcgagacgg tcgcctgggt
     1741 ggcgtcgaag ccccgcctgt ggagctcgtc cagcaggccg ccctggctgg tcacctgcct
     1801 ggtgcgcagc agcgcggcga gggccgcctg gcgctcggcc ttcagcggca tgcgcccacc
     1861 tcctcgagcg cgccggccag cctggcgatc gcctcgtcga cctcctcgac ggtgacgacg
     1921 aggggcgggg ccagccgcag ggcggtcgcg gtgaccgggt tgaccacgag cccgcggccc
     1981 agcgcggcga gcgcggcgtc cgtgtcagcg gtcggggcaa gggcatggcg ggccggctgg
     2041 cccgcgcgtt caacgagctc gcgcagacgc gtgaggtgac gacggacgaa ctcgtccgcg
     2101 tcgcggcggt gatcggccgt gaaggccgcc tgaccgagcg ggccgaggtc cgc
//
LOCUS       DASPYY010000253         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2433372, whole genome shotgun sequence.
ACCESSION   DASPYY010000253 DASPYY010000000
VERSION     DASPYY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433372"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VEP73_03860"
     CDS             complement(<1..470)
                     /locus_tag="VEP73_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25604.1"
                     /translation="MTRSPLSLSQRQAVADGSPLPVRGGGPPASRGEPRTLAAPAGLL
                     PDVAAVTWRAVRGLLREPEAFIPALVIPLFFFVVQLGALGAVARQAFGISSYAAFQLP
                     VSVLFAAASTSGGNALVLDIASGYWDKLSLTPVHRSALVIGTLFGEVFAVLGYSL"
     gene            complement(467..793)
                     /locus_tag="VEP73_03865"
     CDS             complement(467..793)
                     /locus_tag="VEP73_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25605.1"
                     /translation="MGADVIDVAVDRRESGAAQVALEEMQASGELPAGELATTRTGYT
                     LFIPDGPSNIAAVVRGLDRAGIRTGPISVSRPTLDDVFLAATGGRMAAAGDGAAAYDD
                     GARRSA"
     gene            complement(926..1099)
                     /locus_tag="VEP73_03870"
     CDS             complement(926..1099)
                     /locus_tag="VEP73_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25606.1"
                     /translation="MLLTKTQLAELLGVSPRTVDRMVAAGTAPPMTLLPNGRRRWRRG
                     DVRRWLSERRKGG"
     gene            1365..1745
                     /locus_tag="VEP73_03875"
     CDS             1365..1745
                     /locus_tag="VEP73_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25607.1"
                     /translation="MHDQHDDRPPAESTPAMTEAERALAHHRYATRLNLPPQVAHCAS
                     TLERTISAARYAGLGIDDDVCRAMAALDHWADAVRRDVDEGERERAASGADPAVWQRE
                     RERSAWRTLRADIVAGIASPEAGR"
     gene            1748..1987
                     /locus_tag="VEP73_03880"
     CDS             1748..1987
                     /locus_tag="VEP73_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25608.1"
                     /translation="MPGRSRIDDELAQLTWGAKRGYVTHEEYDQRADELCLQADAWAA
                     IRRRNRRGLSPDQLAEYSRRWAAYEANAATRRRRR"
BASE COUNT          300 a          789 c          804 g          247 t
ORIGIN      
        1 aggctgtagc ccagcaccgc gaagacctcg ccgaacagcg tgccgatcac cagcgccgag
       61 cggtgcaccg gcgtgaggct cagcttgtcc cagtagccgc tcgcgatgtc gaggacgagc
      121 gcgttgcctc cggaggtgct ggccgccgcg aacagcaccg acaccgggag ctggaaggcg
      181 gcgtagctcg agatgccgaa ggcctgcctg gccacggcgc cgagggcgcc gagctgcacc
      241 acgaagaaga acagcgggat gaccagggcg gggatgaacg cctctggctc gcgcagcagc
      301 ccgcgcacgg cccgccaggt gacagcggcc acgtccggga ggaggccggc gggagcggcc
      361 agggtgcgcg gctcgccgcg cgaggcgggt ggcccgccgc cgcggaccgg caggggcgag
      421 ccgtccgcga cggcctggcg ctgggacagg gacaggggcg accgggtcat gcggacctcc
      481 gggcaccgtc gtcgtacgcc gccgccccgt cgccggcggc cgccatgcgg ccgccggtgg
      541 cggccaggaa cacgtcgtcc agggtggggc gggagacgct gatcggaccg gtcctgatcc
      601 cggcccggtc gagcccgcgg acgacggcgg cgatgttgga cgggccgtcg gggatgaaca
      661 gtgtgtaccc ggtcctcgtg gtggccagct cgcccgctgg cagctcgccg ctggcctgca
      721 tctcctcgag ggcgacctgg gccgccccgc tctcgcggcg gtcgaccgcc acgtcgatga
      781 cgtccgcgcc cacgtcggcc ttgagcgccg ccggggtccc ctcgcggacg atcctgccct
      841 ggtcgatgat cgcgacccgg tccgcgagct ggtccgcctc ctcgaggtac tgggtggtga
      901 ggaagatgac agcccgctta agtacctacc cgcccttacg gcgctctgag agccagcggc
      961 ggacgtcgcc gcggcgccag cggcgccggc cgttcggcag cagggtcatc ggcggcgccg
     1021 tcccggcggc caccatgcgg tcgaccgtgc gcggcgacac gccgagcagc tcggcaagct
     1081 gcgtcttcgt caagagcatg tcgtcgtcct gttcctgcgg catcacgcga cccccaagtc
     1141 tcggatggtc gcattctgtc ccgtggcgcc ggacggtggc gaacggcacc gccatggtgt
     1201 ctcgcagggt cccatattga cgcacaggga cgcagagggg tagaacggga ctacgcggcg
     1261 ggccggccgg tttgaccccc aagtctcggt tggtccgccg ccatcacgga agcgccccgg
     1321 cgcggctggc accgcccggg gcatggcacg gaaggggtac ttccatgcac gaccagcatg
     1381 acgaccggcc gccggccgag tccacaccag cgatgaccga ggcggaacgg gcgctcgcgc
     1441 accacaggta cgcgacccgg ctgaacctgc cgccccaggt cgcgcactgc gcctcgacgc
     1501 tcgagcggac catctcggcc gcccggtacg ccggcctcgg gatcgacgac gacgtgtgcc
     1561 gggcgatggc ggcgctcgac cactgggccg atgccgtgcg ccgcgacgtc gacgagggcg
     1621 agcgcgagcg ggccgcgtcg ggcgccgacc cggcggtgtg gcagcgcgag cgcgagcggt
     1681 cggcgtggcg gacgctgcgc gccgacatcg tcgcgggcat cgcgtcgccc gaggcaggcc
     1741 ggtagccgtg cccggccgct cgcggatcga cgacgagctg gcccagctca cctggggcgc
     1801 caagcgcggc tacgtcaccc acgaggagta cgaccagcgc gctgacgagc tgtgcttgca
     1861 ggcggacgcg tgggccgcca tccgccgccg caaccgccgc gggctcagcc ccgaccagct
     1921 cgccgagtac tcgcggcgct gggccgcgta cgaggccaac gccgcgacgc gtcgccgacg
     1981 gcgctagccg tcccccggca cccagccggg ggaggctacg ggtgccgggg gacctacggg
     2041 tagatggccg gcccacccgc ggccaggtgc gcccgcgtct gccatcccca gcgcgccagc
     2101 gtcaccgcga ccagcggcga gatgaccgtg ccgcccttcc
//
LOCUS       DASPYY010000254         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1730664, whole genome shotgun sequence.
ACCESSION   DASPYY010000254 DASPYY010000000
VERSION     DASPYY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730664"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VEP73_03885"
     CDS             complement(<1..1013)
                     /locus_tag="VEP73_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYT25609.1"
                     /translation="MPDLGEGGRNRLAGETSPYLLQHAGNPVDWYPWGEEALGRARRE
                     DRPILLSVGYAACHWCHVMAHESFEDPETAAVMNAHFVPVKVDREERPDLDGIYMDAV
                     QAMTGQGGWPMTVFLTPEGGPFFAGTYFPKTDRHGLPGFRKVLLAVADAWRERREDAR
                     SQGEAVVGHIAQGVALEPAAAPLSEDLLRQAFDGLRRTFDPRWGGFGPAPKFPQPMTL
                     EFALRAHLRGYPGALDMVGLTLDRMLLGGIYDQVGGGFHRYSTDQRWLVPHFEKMLYD
                     NAQLLRLAVHAWQVTGRPRYRRAAGETAAYLLRELRQPEGGFSSSQDADSEGHEGKFF
                     VWTA"
     gene            complement(1136..>1583)
                     /locus_tag="VEP73_03890"
     CDS             complement(1136..>1583)
                     /locus_tag="VEP73_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HYT25610.1"
                     /translation="KAHRRMDQADHAMIYVLIAGSYTPFALLALDRAWGLPVLGIVWA
                     VAFGGVAVVILWHRIRFVGITLYLTLGWLAVVTLPSFAGRLGVAKLTLLVAGGVLYTV
                     GAVVLGYQRPNPNPRVFGYHEVWHAFTVAAGLCHYVLILLLVRAAA"
BASE COUNT          232 a          565 c          588 g          198 t
ORIGIN      
        1 gcggtccaga cgaagaactt gccctcgtgc ccctcggagt cggcgtcctg cgacgaggag
       61 aagccgccct ccggctggcg cagctcccgc agcaggtagg cggcggtctc gccggcggcg
      121 cgccggtagc gcggccggcc ggtcacctgc caggcgtgga cggcgagccg caggagctgg
      181 gcgttgtcgt acagcatctt ctcgaagtgg ggcacgagcc agcgctggtc ggtcgagtag
      241 cggtggaagc ccccgccgac ctggtcgtag atcccgccga gcagcatgcg gtccagggtc
      301 agcccgacca tgtcgagcgc gccggggtac ccgcgcaggt gcgcgcggag cgcgaactcg
      361 agcgtcatcg gctgggggaa cttgggcgcc gggccgaacc cgccccaccg cgggtcgaac
      421 gtccgccgga ggccgtcgaa cgcctggcgc agcaggtcct cggagagcgg cgcggccgcc
      481 ggctcgagcg ccaccccctg tgcgatgtgg ccgaccacgg cctcgccctg gctgcgggcg
      541 tcctcgcggc gctcgcgcca ggcgtcggcc accgcgagca gcaccttgcg gaagccgggc
      601 agcccgtgcc tgtcggtctt cgggaagtag gtgccggcga agaacgggcc gccctccggg
      661 gtcaggaaca ccgtcatcgg ccagccgccc tggccggtca tggcctggac ggcgtccatg
      721 tagatcccgt ccaggtcggg gcgctcctcg cggtcgacct tgaccggcac gaagtgggcg
      781 ttcatgaccg cggcagtctc ggggtcctcg aaggactcgt gcgccatgac gtggcaccag
      841 tggcaggccg cgtagccgac cgagagcagg atcggcctgt cctcccggcg cgcccgcccg
      901 agcgcctcct cgccccaggg gtaccagtcg accgggttgc cggcgtgctg cagcaggtac
      961 gggctggtct ccccggccag gcggttgcgc ccaccctcgc cgaggtcagg catgcaggtt
     1021 ctccagggaa gtgtctccag ggaaggggtg ggcgcgagcg gccctcgtcc gtaccagcat
     1081 catgcgcttc caccgtagcc ccgtccatgc cggtcgaggc ggccggccgt cgacctcagg
     1141 cggcggccct gaccaggagc aggatcagga cgtagtggca cagcccggcg gcgaccgtga
     1201 aggcgtgcca gacctcgtgg tagccgaaga cccgcgggtt ggggttcggc cgctggtagc
     1261 cgagcacgac cgcgcccacc gtgtagagca cgccgcccgc gacgagcagc gtgagcttgg
     1321 cgaccccgag ccggccggcg aacgacggca gggtgacgac cgcgagccag ccgagggtca
     1381 ggtagagggt gatcccgacg aaccggatcc ggtgccacag gatcaccacg gcgaccccgc
     1441 cgaaggcgac ggcccacacg atgccgagga ccggcagccc ccaggcccgg tccagggcga
     1501 gcagcgcgaa cggcgtatag ctcccggcga tcagcacgta gatcatcgcg tggtcggcct
     1561 ggtccatccg ccggtgcgcc ttg
//
LOCUS       DASPYY010000255         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1590737, whole genome shotgun sequence.
ACCESSION   DASPYY010000255 DASPYY010000000
VERSION     DASPYY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590737"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VEP73_03895"
     CDS             complement(<1..523)
                     /locus_tag="VEP73_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25611.1"
                     /translation="MNRASAQGPLDPSPQDPEVTLAERWRNFREGVPSALFLLGSAVV
                     LLAVLVLLGMFLAKVASHDAIGQADTRLTRWFAAHRSKDLNEVTHYTTDAAETPTVTI
                     LAVLVVAGAALAWRRWREPLLVAVAVAGEVAIFLMVTLLVDRPRPPVPHLDRAPPTSS
                     FPSGHTAASVALYG"
     gene            729..1907
                     /locus_tag="VEP73_03900"
     CDS             729..1907
                     /locus_tag="VEP73_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYT25612.1"
                     /translation="MPSRRSVRSAAIAGTLGLAGAYAFEAAQYHRAGAKGFELEQPPA
                     PGTPDFARLVEALTAAPLRQGNRVTVLRNGDEIFPAMLAAIQDAEKTVDFATYVYWTG
                     SIAPRFAGALAERARAGVEVNVLLDAVGAARMEPELAERLEEAGATVAWFRPVRWWTL
                     HKLNNRTHRKILVVDGRVGFTGGVGIAEEATQCILSGREHLPDLEGHDDGVQVQVTRS
                     TAQKGSTDAEHLFYAAIACARERLWLTTAYFAPRRAFVEALCAAVARDVDVRVLVNGP
                     HIDAEAVRQAGRRSYDRLLSCGGRIFEYQRTMLHAKVLVVDSAWATVGSINFDNRSFA
                     LNDELNLSVRDPAVVAELEKHFLDDSGDAVELDLARWRARSLSARARELGSAVLRREL
                     "
BASE COUNT          283 a          690 c          738 g          251 t
ORIGIN      
        1 ccccgtagag cgcgacggag gccgcggtgt gcccggacgg gaagctcgag gtgggcgggg
       61 ccctgtcgag gtggggcacg ggggggcggg gccggtccac cagcagggtg accatgagga
      121 agatggccac ctcgccggcg accgcgacgg ccacgagcag cggctcgcgc cagcgccgcc
      181 aggcgagcgc ggcgcccgcg acgaccagca cggcgaggat ggtgacggtg ggcgtctcgg
      241 ccgcgtcggt cgtgtagtgg gtgacctcgt tcaggtcctt cgaccggtgg gcggcgaacc
      301 accgggtcag cctcgtgtcg gcctggccga tggcgtcgtg gctcgcgacc ttggcgagga
      361 acatgccgag cagcaccagc acggcgagca ggacgaccgc gctgccgagc aggaagagcg
      421 ccgaagggac gccctcccgg aagttccgcc agcgctcggc cagggtcacc tcggggtcct
      481 gtggcgacgg gtcgagcggt ccctgcgctg atgctcggtt catcgatgcc cctctcgggt
      541 tgggcctgcg gatcggcccg ggtcctcatc gcgtatgtag ccaagaaggc gagcaaacca
      601 cgaaggcgag cgaatcaccc ggattgggtc cggttgccat gcttgggacc gcggcaccac
      661 gggaactccg ggtgacatcc ggcggacgct acagtagcca gacgtggcag gacgacgaag
      721 gagccgcagt gccgtcgaga cgcagcgtgc gcagcgccgc catcgcggga acgctcgggc
      781 tggccggcgc ctacgccttc gaggccgccc agtaccaccg tgccggggcc aagggcttcg
      841 agctcgagca accgccggcc ccgggcaccc cggacttcgc ccggctggtc gaggcgctca
      901 ccgccgcgcc gctgcgccag ggcaaccggg tcaccgtcct gcgcaacggc gacgagatct
      961 tcccggccat gctcgccgcc atccaggacg cggagaagac ggtcgacttc gcgacctacg
     1021 tctactggac ggggagcatc gccccgcggt tcgcgggcgc cctggccgag cgggcgaggg
     1081 ccggggtgga ggtcaacgtg ctgctcgacg ccgtcggcgc ggccaggatg gagcccgagc
     1141 tggccgagcg gctcgaggag gccggcgcga cggtcgcctg gttccggccc gtgcggtggt
     1201 ggacgctgca caagctgaac aaccgcaccc accgcaagat cctcgtggtc gacggtcggg
     1261 tcggcttcac cggcggggtg gggatcgccg aggaggcgac ccagtgcatc ctcagcggcc
     1321 gcgagcacct gcccgacctg gaggggcacg acgacggcgt gcaggtgcag gtcacccgaa
     1381 gcaccgcgca gaaggggagc accgacgccg agcacctgtt ctacgccgcg atcgcgtgcg
     1441 ccagggagcg gctgtggctg accaccgcct acttcgcgcc gaggcgggcg ttcgtcgagg
     1501 ccctgtgcgc cgcggtcgcc cgcgacgtcg acgtgcgcgt cctcgtgaac ggcccccaca
     1561 tcgacgccga ggccgtccgg caggccgggc ggcgcagcta cgaccggctg ctctcctgcg
     1621 gcgggcgcat cttcgagtac cagcggacca tgctccacgc caaggtgctg gtggtggact
     1681 cggcgtgggc cacggtcggg tcgatcaact tcgacaaccg ctcgttcgcg ctcaacgacg
     1741 agctgaacct ctcggtccgc gaccccgccg tggtcgcgga gctcgagaag cacttcctcg
     1801 acgactcggg cgacgccgtg gagctcgacc tggcccgctg gcgggccagg tccctgtccg
     1861 ccagggccag ggagctcggc agcgcggtcc tccgccggga gctgtgatgg ggggcgtccc
     1921 ggggctcgcg cgcttcagcc tgcccccgcc ttccctgctc cc
//
LOCUS       DASPYY010000256         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_747688, whole genome shotgun sequence.
ACCESSION   DASPYY010000256 DASPYY010000000
VERSION     DASPYY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747688"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VEP73_03905"
     CDS             complement(<1..303)
                     /locus_tag="VEP73_03905"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514931.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HYT25613.1"
                     /translation="MSRPRERPTKVAVVVCDSLGVGEAPDARAYGDEGADTVGHVAAA
                     LGGLSLPRLGAWGLGRLTAVAGVPPADPAAGVVARMAERSAGKDTTTGHWEMMGVVL"
     gene            complement(300..653)
                     /locus_tag="VEP73_03910"
     CDS             complement(300..653)
                     /locus_tag="VEP73_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYT25614.1"
                     /translation="MDQQTTHRLHERLVGERDALRKQLDDLGAHRDAEGIRDPELDEG
                     FADAGQAAAERSSLLRLVQSLRDTLRDVEHALDRMDAGTYGACERCGQPIPDERLEAL
                     PAARLCIRCKRGRHA"
     gene            complement(786..1742)
                     /gene="xerD"
                     /locus_tag="VEP73_03915"
     CDS             complement(786..1742)
                     /gene="xerD"
                     /locus_tag="VEP73_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYT25615.1"
                     /translation="MSPAASRSVRSFLDYLAVERGLAANTLAAYRRDLQRYAGWLAAA
                     GIDDPSQADEDDVVAFLGALRAGSTPVGTRYRASTVARSLAAVRGFHRFLVREHLAGA
                     DPSRDLGGPKVPVSLPKALTVEQVEALLASVAGDDPRALRDRALLETLYAAGLRVSEA
                     TALDVDDVDLDDGAVRAFGKGAKERLVPIGRSARRAVGAYLVRGRPSLAGARSGPALF
                     LNVQGNRLTRQGCWKILRHHAKRAGLPPDVSPHVLRHSFATHLLAGGADVRSVQELLG
                     HASLATTQVYTKVTSDRLREVYLQAHPRARRAGPSPQTASGS"
     gene            complement(1796..>2223)
                     /locus_tag="VEP73_03920"
     CDS             complement(1796..>2223)
                     /locus_tag="VEP73_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYT25616.1"
                     /translation="VLIPGAKAPKLVTREHVKAMRPGAVLVDISIDQGGCFETSHVTT
                     HSDPTYVVDGVVHYCVGNMPGAVPHTSTYALTNVTLPYAIALAAAGVEAAVRADPALA
                     KGVNVWHGELTSEPVAAAHELDWRPLEELLDGAAGQGDR"
BASE COUNT          258 a          827 c          859 g          279 t
ORIGIN      
        1 gagcaccacg cccatcatct cccagtgccc ggtggtggtg tccttgcccg ccgaccgctc
       61 ggccatgcgg gccacgacgc cggccgcggg gtcggcgggc ggcacgccgg cgacggcggt
      121 gagccgcccg aggccccacg ccccgagcct cggcagcgac aggcccccga gggcggcggc
      181 gacgtggccg accgtgtcgg cgccctcgtc gccgtaggcc cgcgcgtcgg gcgcctcgcc
      241 gacgccgagc gagtcgcaca cgaccacggc caccttcgtc gggcgctccc gcgggcggct
      301 cacgcgtgcc tgccccgctt gcagcggatg cagagccggg ccgcgggcag cgcctcgagc
      361 cgctcgtccg ggatcggctg cccgcaccgc tcgcaggcgc cgtacgtgcc cgcgtccatg
      421 cggtcgagcg cgtgctcgac gtcccggagc gtgtcgcgca ggctctgcac cagccggagg
      481 aggctcgacc gctcggctgc cgcctggccg gcgtcggcga agccctcgtc gagctccggg
      541 tccctgatcc cctcggcgtc gcggtgggcg ccgaggtcgt cgagctgctt ccgcagcgcg
      601 tcgcgctccc ccaccagccg ctcgtgcagc cggtgcgtcg tctgctggtc catcccagct
      661 cctcccgtgg gtggtgcgga tctgtcccgg gccggtggcg gacgccgtgc gcggtgctcc
      721 tggtggacgg gcggcccggc agctcgaagc gaagaccaag gcagagtccg gcaagggcta
      781 caagatcatg aaccggaagc ggtctggggt gaaggtccgg ctcgtctcgc gcggggatgc
      841 gcctgcaggt agacctcgcg caggcggtcg gaggtgacct tcgtgtacac ctgggtcgtg
      901 gcgaggctcg catggccgag cagctcctgg acgctgcgga cgtcggcgcc gccggccagc
      961 aggtgggtgg cgaacgagtg gcgcagcacg tgcggcgaca cgtcgggcgg gaggccggcg
     1021 cgcttcgcgt ggtggcggag gatcttccag cacccctgcc tggtgagccg gttgccctgg
     1081 acgttgagga acagcgccgg ccccgacctc gcgccggcca gggacgggcg gccgcgcacc
     1141 aggtaggccc cgacggcgcg ccgcgcggag cggccgatcg ggaccaggcg ctccttggcc
     1201 cccttgccga acgcgcgcac ggcgccgtcg tcgaggtcga cgtcgtcgac gtcgagcgcc
     1261 gtcgcctcgc tgacccgcag gcccgcggcg tacagggtct cgagcagggc ccggtcgcgc
     1321 agcgccctcg ggtcgtcgcc agccaccgag gcgagcagcg cctcgacctg ctcgacggtg
     1381 agcgccttgg gcagcgacac cggcaccttg gggccgccga ggtcgcggga cgggtcggcc
     1441 ccggccaggt gctcgcggac caggaaccgg tggaagccgc gcaccgcggc gaggctgcgg
     1501 gccacggtcg aggcgcggta gcgggtgccg accggcgtgc tgccggcccg cagcgccccg
     1561 aggaacgcga ccacgtcgtc ctcgtccgcc tggctcgggt cgtcgatccc ggccgccgcc
     1621 agccagccgg cgtaccgctg caggtcgcgc cggtaggcgg ccagcgtgtt ggcggccagg
     1681 ccgcgctcga cggccaggta gtcgaggaag ctccgcacgg accgggaggc ggccgggctc
     1741 accgggcgct gccagccccc ggagccgtcc accacctgcg ctgccacggc cgcgctcagc
     1801 ggtcaccctg gccggccgcg ccgtcgagga gctcctcgag cgggcgccag tcgagctcgt
     1861 gggcggctgc caccggctcg gaggtcagct cgccgtgcca gacgttcacg cccttggcga
     1921 gcgccgggtc cgcgcgcacg gccgcctcga cccccgcggc ggcgagcgcg atggcgtagg
     1981 gcagggtcac gttcgtgagc gcgtaggtcg aggtgtgggg gacggccccg ggcatgttgc
     2041 cgacgcagta gtggaccact ccgtccacca cgtaggtggg gtcggagtgg gtcgtcacgt
     2101 ggctcgtctc gaagcagccg ccctggtcga tcgagatgtc gacgagcacg gcgcccggcc
     2161 gcatcgcctt cacgtgctcc ctggtgacga gcttgggcgc cttggccccg gggatcagga
     2221 ccg
//
LOCUS       DASPYY010000257         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1591157, whole genome shotgun sequence.
ACCESSION   DASPYY010000257 DASPYY010000000
VERSION     DASPYY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591157"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VEP73_03925"
     CDS             complement(<1..877)
                     /locus_tag="VEP73_03925"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018737729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HYT25617.1"
                     /translation="MAIEDRVRELAGSVEPLDERAGAAASARQEQLTKPAGSLGRLEA
                     LGTRLAAIAGRCPPPVPERPAVVVAAGDHGVLAQGVSPWPREVTATMAAGIATGHAAV
                     NALAGVVGAQVVVLDVGVATPLPPHPMLRRARVRAGTGDLSEGPAMTRAEAARALLAG
                     AGVAEELVGAGADLLVTGDMGIGNTTPAACLAAAFTGRPAGEVTGRGTGIDDATLELK
                     VKVVQGALARHAPDPADPLGVLAALGGLEHAALAGLALAGAALRVPVVLDGVSANVAA
                     LAACRLAPLARGYLVA"
     gene            complement(942..1625)
                     /locus_tag="VEP73_03930"
     CDS             complement(942..1625)
                     /locus_tag="VEP73_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HYT25618.1"
                     /translation="MTPALGRGGLVVDLHTVHRCLSSAVLGGGLGLVRTWLDLQVPPD
                     YARTDPEAHLAEAAAGLPGPVVGMLTAAPVARFRQGSAGRARAVATVGVGHPLAAAGT
                     RPRAAPAGAGSRAAPGAGTINLLVVVDDPLTDAGLAGALQTAVEAKAQALAAARIPAV
                     NAAGFATGTATDSVCVACPPGARSPFAGPATRTGGDLARAVYEAVIAGAAAAGRAAAG
                     TQRPAHPVP"
     gene            complement(1622..3277)
                     /gene="cobC"
                     /locus_tag="VEP73_03935"
     CDS             complement(1622..3277)
                     /gene="cobC"
                     /locus_tag="VEP73_03935"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:NF005915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="HYT25619.1"
                     /translation="MALHLVLGGVRSGKSELAERLATAAGAPVCYVATGSAGDPEMAE
                     RIARHASRRPAGWRTVETDDPAWVVGAAPAGGVQGRDRGVAGRAPARAGDTVLVDGVA
                     PWLARLMADEGLWTGEEVAALGPGGQAAWARAVDRVRAFAAAAAARPGLTVVAAEESG
                     LGLLPRGAAARRYLDLAGEAAQVLAAAAGRVQLAVAGRPVELGGAAPHRPAGDPVPLP
                     LRLHGDTMVPAGHLDFAVNVVPGGPPPPLRAELARALERAGAYPDEGPATVALADRHG
                     RAPAEVLAANGSVEAFWLLACVLRPGRAVVVHPSFTEPEAALRAAGHRVERAFRHPED
                     YALDPGAIPGDADLVVVGNPNNPTGTLDPAAALAALARPGRVLVVDEAFMEFCPGEPE
                     SLAARAELPGLVVVRSLTKLWGLAGIRAGYLLAPPDLAAALRAARQPWSVNAMACAAL
                     AACAADAATPARVAAEVGAARAELAAGLAALPGVRVWPSAANFLLLRVPDGPAARAGL
                     ARQGIAVRRADTFPGLTPDHLRVAVRCPEDNRRLVAALAEALR"
     gene            complement(3277..4191)
                     /locus_tag="VEP73_03940"
     CDS             complement(3277..4191)
                     /locus_tag="VEP73_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HYT25620.1"
                     /translation="MNRPALLVAGHGSRDRAGAAELEVLLDRVRALDPGLAVEGGFIE
                     LSSPPVPVAADRLVAGGARDVVVVPLMLLAAGHAKNDVPALLARVRLEHPGVRFRYAR
                     DLGIHPDLLALADERLGAVVAAAERPATAVLVCGRGSSDPDANADLHKVARLLWEGRP
                     YPMVEVCYVGITGPRLPEGLERCRRLGALRIVTLPYFLFTGRLERRIRAECAAFGAAH
                     PALDVRVAGYLGPDARVARLVLARWREAVVGDPRMNCDLCIHRVALPGFEASVGAPAT
                     PHHHPADAHAGRAGPDAHVGRAGPGGLR"
     gene            complement(4188..>4379)
                     /locus_tag="VEP73_03945"
     CDS             complement(4188..>4379)
                     /locus_tag="VEP73_03945"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HYT25621.1"
                     /translation="VVGLPVGFVGAVEAKAALVASGLPAVTNRSAKGGAAVAAAALNA
                     LLYRSACLRVDPPPGGGGR"
BASE COUNT          480 a         1686 c         1817 g          396 t
ORIGIN      
        1 cggcgacgag gtagccccgg gcgagtggcg cgagccggca ggcagccagg gcggcgacgt
       61 tggcgctgac gccgtcgagc accaccggga cgcgcagcgc cgcgccggcg agcgcgagcc
      121 cggccagggc ggcgtgctcg agcccgccga gggcggcgag caccccgagc gggtcggccg
      181 ggtcgggcgc gtgcctggcg agggcgccct ggaccacctt gaccttcagc tcgagggtgg
      241 cgtcgtcgat gccggtgccc cgcccggtga cctcgcccgc gggccggccg gtgaacgcgg
      301 cggccaggca cgcggccggg gtcgtgttgc cgatgcccat gtcgccggtc acgagcaggt
      361 cggcgcctgc gcccacgagc tcctcggcca cgccggcgcc ggcgagcagg gcgcgggccg
      421 cttcggcgcg ggtcatggcc gggccctcgg acaggtcgcc ggtgccggcg cggacgcgcg
      481 cgcggcgcag catcgggtgc ggcggcagcg gcgtggcgac cccgacgtcg agcacgacga
      541 cctgcgcgcc gaccaccccg gcgagcgcgt tgacggcggc gtggccggtg gcgatgccgg
      601 ccgccatggt cgcggtgacc tcgcgcggcc acggggacac gccctgggcg aggactccgt
      661 ggtcgccggc ggcgaccacg acggcgggcc gctccggcac cggcggcggg cagcggccgg
      721 cgatggcggc caggcgggtg ccgagcgcct cgagccgccc gaggctccct gccggcttgg
      781 tgagctgctc ctggcgggcg ctggcggcgg ccccggcccg ctcgtcgagc ggctcgacgc
      841 tgccggcgag ctcccggaca cggtcctcga tggccatggt cggtgcacct acctctcgct
      901 aaggttgggt cccgcaggga cgggtctcac gggattgtgt ctcacgggac cgggtgggca
      961 gggcgctgcg tccccgcggc ggcccgcccg gccgccgccg cgccggcgat gaccgcctcg
     1021 tagacggcgc gggcgaggtc gccgccggtg cgggtggccg gcccggcgaa cggcgagcgg
     1081 gcgccgggcg ggcaggcgac gcagaccgag tcggtggccg tgccggtcgc gaacccggcg
     1141 gcgttcacgg ccgggatccg ggcagccgcg agcgcctgcg ccttggcctc gaccgccgtc
     1201 tgcagggcgc cggccagccc ggcgtcggtg agcgggtcgt cgacgacgac gagcaggttg
     1261 atggtgccgg cgcctggcgc cgcccgggac ccggctccag ctggcgccgc ccggggccgg
     1321 gtcccggccg cggcgagcgg gtgcccgacg ccgacggtgg cgaccgcgcg ggcgcggcct
     1381 gcactgccct ggcggaaccg ggcgaccggg gcagcggtga gcatgccgac caccggcccg
     1441 ggcaggccgg cggccgcctc ggccaggtgc gcctcggggt cggtgcgcgc gtagtccggg
     1501 gggacctgca ggtcgagcca tgtccggacg agcccgagcc cgcctccgag cacggccgag
     1561 gacaggcagc ggtggaccgt gtgcaggtcg acgaccaggc cgccgcgccc gagcgccggt
     1621 gtcaccggag cgcctcggcg agcgcggcga cgagccgccg gttgtcctcg gggcacctga
     1681 ccgcgacgcg gaggtggtcg ggggtgaggc ctgggaaggt gtcggcacgg cgcaccgcga
     1741 tcccctgcct ggcgagcccg gcgcgggccg ccgggccgtc gggcacccgg aggagcagga
     1801 agttcgcggc cgatggccag acccgcacgc cgggcagggc ggccaggccg gcggcgagct
     1861 ccgcccgggc ggcgccgacc tcggccgcca cccgcgccgg ggtggcggcg tccgcggcgc
     1921 acgccgcaag ggcggcgcag gccatggcgt tgacgctcca cggctggcgg gcggcgcgca
     1981 gggcggcggc caggtcgggc ggggcgagca ggtagccggc ccggatcccg gcgaggcccc
     2041 acagcttggt caggctgcgg accaccacca ggccgggcag ctcggcccgg gcggcgaggg
     2101 actcgggctc gcccgggcag aactccatga acgcctcgtc gaccacgagc acccgccctg
     2161 ggcgcgcgag cgcggccagg gcggcggccg ggtccagcgt gcccgtcggg ttgttggggt
     2221 tgccgaccac gacgaggtcg gcgtcgccgg ggatggcgcc ggggtcgagg gcgtagtcct
     2281 ccgggtggcg gaaggcacgc tccacccggt ggccggccgc gcgcagggcc gcctcgggct
     2341 cggtgaacga ggggtggacc accacggccc tgccagggcg aaggacgcac gcgagcagcc
     2401 agaacgcctc cacgctgccg ttggcggcga gcacctcggc gggtgcgcgg ccgtgccggt
     2461 cggccagcgc gacggtcgcc gggccctcgt ccgggtaggc gcccgcacgc tcgagcgccc
     2521 gcgccagctc ggcccgcagc ggcggcggcg gccccccggg cacgacgttg accgcgaagt
     2581 ccaggtgccc ggcggggacc atggtgtccc cgtgcagccg cagcgggagc gggaccgggt
     2641 ccccggcagg ccggtgggga gcggcgccgc ccagctcgac cggccggccg gccacggcaa
     2701 gctgcacccg cccggcggcg gcggcgagga cctgggccgc ctcgccggcc aggtcgaggt
     2761 agcggcgcgc ggcggcgccc cgggggagca ggccgaggcc gctctcctcg gcggccacca
     2821 ccgtcagccc cgggcgggcc gccgccgcgg cggcaaacgc ccgcacccgg tcgacggcgc
     2881 gcgcccaggc cgcctgcccg cccggcccga gggcggccac ctcctcgccc gtccacagcc
     2941 cctcgtcggc catgagcctg gcgagccacg gcgcgacccc gtcgaccagg accgtgtccc
     3001 ccgccctggc cggcgcgcgc ccggcgacgc cccggtcgcg accttgcacc ccgccggccg
     3061 gggcggcgcc gaccacccag gccgggtcgt cggtctcgac cgtgcgccag ccggcgggcc
     3121 gccggctcgc gtggcgggcg atccgctcgg ccatctccgg gtcgcccgcg gacccggtcg
     3181 ccacgtagca gaccggcgcg ccggccgccg tggccaggcg ctcggccagc tcgctcttgc
     3241 ccgagcggac gccgccgagg accaggtgca gggccatcag cggaggccgc cgggcccggc
     3301 ccggccaacg tgggcgtcgg ggccggcccg gccggcgtgg gcgtcggcgg gatggtggtg
     3361 gggcgttgcc ggcgccccga cgctggcctc gaagccgggc agcgcgaccc ggtggatgca
     3421 caggtcgcag ttcatgcggg ggtccccgac gaccgcctcg cgccagcggg cgagcacgag
     3481 ccgggcgacc cgcgcgtcgg ggccgaggta gccggcgacg cgcacgtcga gtgcggggtg
     3541 ggcagccccg aacgcggcgc actccgcccg gatgcgccgc tcgagccggc cggtgaacag
     3601 gaagtacggc agggtcacga tgcgcagggc gccgaggcgc cggcagcgct ccagcccctc
     3661 gggcaggcgc ggcccggtga tcccgacgta gcacacctcg accatcgggt agggccggcc
     3721 ctcccagagc agccgggcca ccttgtggag gtcggcgttg gcgtccgggt cggagctgcc
     3781 ccgcccgcag acgagcaccg cggtcgcggg ccgctccgcc gcggcgacca ccgcgccgag
     3841 gcgctcgtcc gccagcgcga gcaggtcggg gtggatgccg aggtcgcggg cgtagcggaa
     3901 gcggaccccg ggatgctcga ggcggacccg ggcgagcagc gcgggcacgt cgttcttggc
     3961 gtggccggcg gcgagcagca tgagaggcac gaccacgacg tcgcgggcgc ccccggccac
     4021 gagccggtcg gccgccaccg gcaccggcgg ggacgacagc tcgatgaacc cgccctccac
     4081 ggcgaggccg gggtcgagcg cccgcacccg gtcgagcagg acctcgagct cggcggcgcc
     4141 ggcccggtcc cggctgccgt gcccggcgac cagcagcgcc gggcggttca ccggccccca
     4201 cccccgggtg gcgggtcgac tcggaggcac gccgaccggt agagcagggc gttcagcgcg
     4261 gcggcggcca cggccgcgcc gcccttcgcg ctccggttgg tgaccgcggg caggccgctg
     4321 gccacgagcg ccgccttggc ctcgaccgcg ccgacgaacc cgaccggcag cccgacgac
//
LOCUS       DASPYY010000258         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1451627, whole genome shotgun sequence.
ACCESSION   DASPYY010000258 DASPYY010000000
VERSION     DASPYY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451627"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VEP73_03950"
     CDS             complement(<1..639)
                     /locus_tag="VEP73_03950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25622.1"
                     /translation="MDIRPLRPDDSLEDDLDLARRSFGPFDDAGRARRLAEAGAAVGD
                     GRYFAAFDGPRLLAVAGFHDLRQWWHGRPLRMAGVGGVRVAPEARGRGVGTALMTELL
                     RVIADRGYPLSVLFPATAPVYRSLGWELAGGFYEATVPTRSLAALPPAEVPAGPLPAL
                     RRAGPGDADQVLAVIAAVHESARHCGPATSDAAAARRWWLDNEDVFCYLAPDG"
     gene            complement(643..1395)
                     /locus_tag="VEP73_03955"
     CDS             complement(643..1395)
                     /locus_tag="VEP73_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT25623.1"
                     /translation="MSGHSKWATTKHKKAVVDARRGKLFAKLIKNVEVAARTGGGDPA
                     GNPTLYDAIQKAKKSSVPNENIDRAVRRGAGLEAGGATYETVTYEGYGPGGVAILVEC
                     LTDNKNRAASDVRTALTRNGGSMADPGSVSYLFNRKGVVLVPKAGGVSEDDVLGAVLD
                     ADAEEINDLGESFEVVSEAHDLVAVRQALQDAGIDYESAEAAFLPSVEVPLDEDHAKR
                     VFRLIEAVEDSDDVQDVYANYSVSDDVLEKLG"
     gene            complement(1392..2000)
                     /gene="pdxT"
                     /locus_tag="VEP73_03960"
     CDS             complement(1392..2000)
                     /gene="pdxT"
                     /locus_tag="VEP73_03960"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HYT25624.1"
                     /translation="MPATQQSPVVGVLALQGDVAEHLRALREAGARPVPVRRPGELDA
                     VDGLVIPGGESTTMWKLAVIFELMEPLRKRLGAGLPAFGSCAGMIMLADCLEDGIEGQ
                     ETLGGIDMTVRRNAFGRQVDSFESDIALRGIAGPDFRAVFIRAPWVERTGRDVEVVGT
                     ESGTGRIVAVRQGPALATAFHPELTPDLRIHKLFVDMVKERS"
BASE COUNT          288 a          791 c          697 g          297 t
ORIGIN      
        1 gccgtccggc gccaggtagc agaacacgtc ctcgttatcc agccaccagc gccgggccgc
       61 ggcggcgtca ctggtggccg ggccgcagtg cctggccgac tcgtgcaccg ccgcgatcac
      121 cgccaggacc tggtcggcgt cgcccgggcc cgcccggcgc agcgcgggca gcggccccgc
      181 gggcacctcg gcgggcggga gcgcggccag cgacctggtc gggaccgtgg cctcgtagaa
      241 gccgcccgcc agctcccagc ccagcgaccg gtagaccggc gcggtggccg ggaagagcac
      301 ggacagcggg tagccccggt cggcgatcac acggagcagc tcggtcatca gcgcggtccc
      361 gacgccgcgg ccgcgcgcct cgggcgcgac cctgaccccg cccacgccgg ccatccgcag
      421 cggccggccg tgccaccact ggcgcaggtc gtggaacccg gctacggcca gcagccgcgg
      481 cccgtcgaag gccgcgaagt accggccgtc gccgaccgcc gccccggcct cggctagccg
      541 ccgggcgcgg ccagcgtcgt cgaaagggcc gaacgaccgg cgcgccaggt caagatcgtc
      601 ctcgaggctg tcatccgggc gcagcgggcg gatgtccacg ggtcagccga gcttctccag
      661 cacgtcgtcg gagaccgagt agttcgcgta gacgtcctgc acgtcgtcgg agtcctcgac
      721 ggcctcgatc agccggaaca cgcgcttggc gtggtcctcg tccagcggca cctcgaccga
      781 gggcaggaac gcagcctccg ccgactcata gtcgatcccg gcgtcctgca gcgcctgacg
      841 caccgcgacc aggtcgtgcg cctcgctgac cacctcgaac gattcgccga ggtcgttgat
      901 ctcctcggca tcggcgtcga gcacggcacc caggacatcg tcctcgctca ccccgcccgc
      961 cttggggacg agcaccacgc ccttccggtt gaacaggtag gacaccgagc ccgggtcggc
     1021 catcgagccg ccgttgcggg tgagcgcggt gcgcacgtcg gacgcggccc ggttcttgtt
     1081 gtcggtcagg cactccacca ggatcgccac gccgccgggg ccgtacccct cgtaggtgac
     1141 cgtctcgtac gtcgcgccgc cggcttcgag ccccgcgccg cggcggaccg cccggtctat
     1201 gttctcgttg gggacagagc tcttcttcgc cttctggatg gcgtcataca gcgtggggtt
     1261 gccggccgga tcgccgccgc cggtcctcgc cgccacctcc acgttcttga tcagcttggc
     1321 gaacagcttg ccccgcctgg cgtcgacgac ggccttcttg tgcttggtgg tcgcccactt
     1381 cgaatggccg ctcatgatcg ttccttcacc atgtctacaa aaagcttgtg gatacgcagg
     1441 tccggcgtga gctcggggtg gaacgcggtg gcgagcgccg gaccctggcg gaccgcgacg
     1501 atcctacccg tgccggactc ggtcccgacg acctcgacgt cccggccggt ccgctctacc
     1561 cacggcgccc ggatgaacac cgcccggaag tcggggccgg cgatcccgcg cagcgcgatg
     1621 tcgctctcga acgagtccac ctggcggccg aacgcgttcc gccggacggt catgtcgatc
     1681 ccgccgagcg tctcctgccc ctcgatcccg tcctccaggc agtcggccag catgatcatt
     1741 ccggcgcagg agccgaaggc aggcagtccc gctccgaggc gtttacgcaa cggctccatc
     1801 aactcgaaga tcaccgcgag cttccacatc gtggtggact cgccgccggg aatgaccagg
     1861 ccgtcgaccg cgtcgagctc gcctggcctg cgcaccggca cgggacgggc tcccgcttcg
     1921 cgcagggccc gcagatgctc ggcgacgtcg ccctggagtg cgaggacgcc gaccacgggc
     1981 gactgctgag tggctggcac gctccccctc cgttaatgcc cttgagctgg ctccgctacc
     2041 agccgcggcc cgcgtagcgc tgctcgtcag gga
//
LOCUS       DASPYY010000259         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1591673, whole genome shotgun sequence.
ACCESSION   DASPYY010000259 DASPYY010000000
VERSION     DASPYY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591673"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VEP73_03965"
     CDS             complement(<1..1184)
                     /locus_tag="VEP73_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT25625.1"
                     /translation="MADGVRPHDPGAPPWPHDPGAALGPGTGGWLGRSLPRREDARLV
                     TGRARFLGDLWRPGVLHCVFVRSTEAHGRLGPASLGAVREAPGVVAAFAAADLGLADI
                     PGSTGRGPQAPAMRRPVLPRDRVRYVGEPVAAVVARTREAGEDAAERLDLAVEPEEVV
                     AGAGTALTDRVLLFPEAGTNVVARTTLPERAPGPGPGAWPVRVVVETCNQRLAPAPIE
                     PLGILAEPDAGGVTVHCGHQAPHRLRDQLARLLRLDPASVRVVVPEVGGAFGLKGMLF
                     PEYLVVVAAALRLGRPVLWSEARHDRFLVGTHGRGQRHRVVLGGDERGRIRAARVEIL
                     ADLGAYPHNGSAVPLFTRYLAGGPYAIDDLQVTTTMVVTNRAPTGSYRGAGRPEAAYA
                     IER"
     gene            complement(1177..2757)
                     /locus_tag="VEP73_03970"
     CDS             complement(1177..2757)
                     /locus_tag="VEP73_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT25626.1"
                     /translation="MRERFDVVVAGAGHNSLVAAAYLARAGLRCLVLEARPEIGGNTS
                     VEEGPLPGFWQDACATAHSIFQTSPIWRDDELRLADYGLEYLRPEPAAHVALGDGSWF
                     TQWTDLDRTCEELARFCKRDADAYRRMLREYESVAPLVSRHRYTPIGFGPSLDERLAA
                     HPEGFRWRRRLAMSSWELIRDTFRDWHNRAFMLWMATQTVQPADRPGTGQLAYSIVYG
                     RQRHSWAIPKGGSAALPRALARIVEEHGGVVRTGTPVTGLVLERGRCTGVETAGGERY
                     LARKAVLSSIHVKHLVQMAPRETWGEEFWYGVETWQPGVTLFASYYATTRAPAFEVEG
                     GTVAPVASGVAPSVERLLRIGDDFRRSHVDTHDPVLLVLCPTTADPSRAPAGRHTLKI
                     VAMLPYDLTGGPRRWDELKDEVAASHLAQLRRFAPNLTEETILARSVRSPLDLERFNA
                     HNWHGSCHGGDLSPAQSGELRPAPGWAQHRTPVHGLYQTGATTHPGGSVSGGPGRNAA
                     AVLLHDLGMRLEQVVAHG"
     gene            complement(2779..>3337)
                     /locus_tag="VEP73_03975"
     CDS             complement(2779..>3337)
                     /locus_tag="VEP73_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT25627.1"
                     /translation="LAVDGPGQGEAEYELPIEPAYERVVAACFDWLERRPEVDPARLG
                     VFGVSLGGYYAARAAAFEQRVRAAVDLAGPYAFDWELDHLPAASRLAFQHRSGAADAG
                     QARERAAALTLAGAAARIAVPLLVLHGGRDRLIPREQAERLAAEAPGGELVMFEDGNH
                     GLTNHVFESRTLMADWLAERLRPAA"
BASE COUNT          408 a         1259 c         1289 g          381 t
ORIGIN      
        1 cgctcgatgg cgtaggcggc ctcgggacgg ccggcgccac ggtaggaacc ggtcggagcg
       61 cggttggtca ccaccatcgt cgtggtcacc tgaaggtcgt cgatggcgta gggaccgccg
      121 gccaggtagc gggtgaacag cgggacggcc gagccgttgt gggggtaggc cccgaggtcg
      181 gcgaggatct cgacccgggc cgcgcggatg cgcccgcgct cgtccccgcc gagcacgacg
      241 cggtggcgct gcccgcgccc gtgcgtgccg accaggaagc ggtcgtggcg tgcctcgctc
      301 cacagcaccg ggcggccgag gcgcagcgcc gccgcgacga cgaccaggta ctccgggaag
      361 agcatcccct tcaggccgaa cgcgccgccc acctcgggga cgaccacgcg gaccgaggcg
      421 ggatccagcc gcagcagccg cgcgagctgg tcgcggaggc ggtgcggcgc ctggtggccg
      481 cagtggacgg tcacgccgcc ggcgtccggc tcggccagga tgccgagcgg ctcgatcggc
      541 gccggggcga ggcgctggtt gcaggtctcg acgacgaccc gcaccggcca cgcgccgggc
      601 cccgggcccg gagcgcgctc gggcagggtg gtgcgggcga cgacgttggt cccggcctcg
      661 gggaacagca gcacccgatc ggtcaaggcc gtgccggcgc ccgcgaccac ctcctccggc
      721 tcgacggcga ggtcgagccg ctccgcggcg tcctccccgg cctcgcgggt ccgcgcgacc
      781 acggcggcga cgggctcgcc cacgtagcgg acccggtcgc gaggcagcac cggccggcgc
      841 atcgccgggg cctgcgggcc gcggccggtg ctgccgggga tgtcggccag cccgaggtcg
      901 gcggcggcga aggcggcgac cacccctggg gcctcgcgga ccgcgccgag cgacgcgggc
      961 ccgagccggc cgtgcgcctc ggtgctgcgc acgaagacgc agtgcagcac gccgggccgc
     1021 cacaggtcgc cgaggaagcg tgcccgcccg gtcacgagcc gcgcgtcctc gcgccgcggg
     1081 agcgagcggc cgagccaccc gccggtgccc ggccccaggg ccgcgccagg gtcgtgcggc
     1141 cagggaggcg cgccggggtc gtgcggccgg acgccgtcag ccatgcgcca ccacctgctc
     1201 gagccgcatg ccgaggtcgt ggaggagcac cgcggccgcg ttcctcccgg gaccgcccga
     1261 caccgacccg cccgggtggg tcgtggcgcc ggtctggtag aggccgtgga ccggcgtgcg
     1321 gtgctgcgcc cagcccgggg ccgggcgcag ctcgcccgac tgtgccgggc tcaggtcgcc
     1381 gccatggcag gagccgtgcc agttgtgggc gttgaaccgc tcgaggtcca gcgggctcct
     1441 gacgctccgc gccaggatcg tctcctcggt caggttcggc gcgaagcggc ggagctgcgc
     1501 gaggtgcgac gcggccacct cgtccttgag ctcgtcccag cgccgcgggc cccctgtgag
     1561 gtcgtagggc agcatcgcga cgatcttgag cgtgtggcgg ccggcgggcg cccgcgacgg
     1621 gtcggcggtg gtcgggcaga gcacgagcag caccgggtcg tgggtgtcca cgtgcgagcg
     1681 ccggaagtcg tcgccgatgc gcagcagccg ctcgaccgag ggcgcgacgc cgctcgccac
     1741 gggcgcgacc gtgccgccct cgacctcgaa cgccggcgcc cgggtggtcg cgtagtagga
     1801 cgcgaacagc gtgacgccgg gctgccaggt ctccacgccg taccagaact cctcgcccca
     1861 tgtctcacgg ggcgccatct gcaccaggtg cttgacgtgg atgctcgaga gcaccgcctt
     1921 gcgcgccagg taccgctcgc cgccggcggt ctccacgccg gtgcagcggc cgcgctcgag
     1981 cacgaggccg gtgaccggcg tgccggtccg caccaccccg ccgtgctcct cgacgatccg
     2041 cgcgagcgcg cgcggcagcg ccgccgaccc gcccttcggg atcgcccagc tgtgccgctg
     2101 gcggccgtac acgatcgagt acgcgagctg gccggtcccg ggccggtccg ccggctgcac
     2161 cgtctgggtg gccatccaga gcatgaacgc ccggttgtgc cagtcccgga acgtgtcgcg
     2221 gatgagctcc caggagctca tcgccaggcg ccgccgccag cggaacccct ccgggtgggc
     2281 ggcgagccgc tcgtcgaggg acgggccgaa gccgatcggg gtgtagcggt ggcggctgac
     2341 cagcggcgcg accgactcgt actcgcgcag catgcgccgg taggcgtcgg cgtcgcgctt
     2401 gcagaacctg gcgagctcct cacaggtccg gtccaggtcc gtccactgag tgaaccagct
     2461 gccgtccccg agcgcgacgt gtgcggcggg ctcgggccgg aggtactcga ggccgtagtc
     2521 ggccaagcgc agctcgtcgt cccgccagat cgggctcgtc tggaagatcg agtgcgccgt
     2581 cgcgcaggcg tcctgccaga agcccggcag cggtccctcc tcgacgctgg tgttgccgcc
     2641 gatctccggc cgcgcctcga gcaccaggca gcgcaggccc gccctggcca ggtacgcggc
     2701 ggcgaccagg ctgttgtgcc ccgcacccgc gacgacgacg tcgaagcgct cgcgcatccg
     2761 ccctcctcgc ccgtgcggtc aggccgcggg ccgcagccgc tcggcgagcc agtccgccat
     2821 gagggtccgc gactcgaaga cgtggttggt gaggccgtgg ttgccgtcct cgaacatgac
     2881 cagctcgccg cctggcgcct cggcggcgag ccgctccgcc tgctcccgcg ggatcagccg
     2941 gtcccggccg ccgtgcagca ccagcagcgg gacggcgatg cgggccgcgg cgcccgcgag
     3001 ggtgagcgcc gcggcgcgct cgcgggcctg gccggcgtcg gccgcgcccg accggtgctg
     3061 gaaggcgagc cggctcgccg ccggcaggtg gtccagctcc cagtcgaagg cgtacgggcc
     3121 ggcgaggtcc acggcggccc gcacgcgctg ctcgaaggct gccgcacggg ccgcgtagta
     3181 gccgccgagg ctgacgccga agacgccgag ccgcgccggg tcgacctcgg ggcggcgctc
     3241 cagccagtcg aagcaggccg cgaccacccg ctcgtaggca ggctcgatcg gcagctcgta
     3301 ctcggcctcg ccctggccag ggccgtcgac cgccagg
//
LOCUS       DASPYY010000260         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1310943, whole genome shotgun sequence.
ACCESSION   DASPYY010000260 DASPYY010000000
VERSION     DASPYY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310943"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VEP73_03980"
     CDS             complement(<1..287)
                     /locus_tag="VEP73_03980"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HYT25628.1"
                     /translation="MSYQPQTDSDVRRMLEALGLTDVEQLFEPIPDELRAPTDLDLPR
                     PHAEQEVAAELALLAARNRHLDELTCFLGAGVYDHYVPAAVWYLAGRGEFAT"
     gene            complement(295..846)
                     /locus_tag="VEP73_03985"
     CDS             complement(295..846)
                     /locus_tag="VEP73_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYT25629.1"
                     /translation="MVDRSSPGAAGPGFRGPTVYKLVGISYRQLDYWTRTGLVTPSVR
                     AADGSGSHRLYSFGDLVELRIIKRLLDAGISLQRIREAIGYLRDESAGRPLTDVTLIS
                     DGRRIYACHSGEEVIDVLSHGQAVFGIAVGRVWADTEGDVARLPTDERAGAGAGQPSL
                     PGLDPIAQASGDPMPPPPSRARG"
     gene            complement(940..1416)
                     /locus_tag="VEP73_03990"
     CDS             complement(940..1416)
                     /locus_tag="VEP73_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HYT25630.1"
                     /translation="MIELTLVGVRIELPTQTPIVLLKERDGERFLPIWIGNVEATAIA
                     FALQGVATARPLTHDLLKNLLDELVVSIDRVVITELKEGTFYATIELRQNGSRFSVSS
                     RPSDAIALAVRANTPIFAEEGVLSEASILIKESDDEEREVKKFREFLDQVSPEDFE"
     gene            complement(1545..>2159)
                     /locus_tag="VEP73_03995"
     CDS             complement(1545..>2159)
                     /locus_tag="VEP73_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25631.1"
                     /translation="GTRRATIPGPAGSRRFPNRNDLLFTYRWTEGGKTGFTNLARRCL
                     VASARRGGRRLVAVALGSPNAFTDVRALFEYGFNALVRVRLAARGRTVTVQGGRWQVG
                     ADVDALVRRDLLARVAAVPAPSGGARPGPAPAGATGAGATDAAGTGAPPRVWLAAGGI
                     LLAPLPLGPNGGAAPSGGGGFRAAPVPAGSASPVVDPFLTQGGP"
BASE COUNT          300 a          760 c          831 g          268 t
ORIGIN      
        1 gtggcgaact ccccccggcc ggcgaggtac cagaccgccg cgggcacgta gtggtcgtac
       61 accccggcac cgaggaagca ggtgagctcg tcgaggtgcc ggttgcgggc cgcgaggagg
      121 gccagctcgg cggccacctc ctgctcggcg tggggccggg gcaggtccag gtcggtcggg
      181 gcgcgaagct cgtccgggat cggctcgaag agctgctcga cgtcggtgag cccgagcgcc
      241 tcgagcatcc ggcgcacgtc gctgtcggtc tggggctggt agctcacggt gccctcaccc
      301 gcgggcgcgg ctgggcgggg gcggcatcgg gtccccggac gcctgggcga tcgggtcgag
      361 gccagggagg gacggctggc cggcgccggc cccggcgcgc tcgtcggtgg gcaggcgggc
      421 gacgtcgccc tcggtgtcgg cccacacccg gccgacggcg atgccgaaca cggcctgccc
      481 gtgcgacagg acgtcgatca cctcctcgcc cgagtggcag gcgtagatgc gcctgccgtc
      541 ggagatcagg gtgacatcgg tcagcggcct gcccgccgac tcgtcgcgca ggtagccgat
      601 cgcctcgcgg atccgctgca gggagatccc ggcgtcgagc aggcgcttga tgatgcgcag
      661 ctcgaccagg tcgccgaagc tgtacagtcg gtggctgccc gatccgtcgg cggcgcgcac
      721 cgacggggtg accaggccgg tcctggtcca gtagtcgagc tgccggtagg agatgccgac
      781 cagcttgtag accgtcggcc cgcggaagcc gggacctgcc gctccggggg aagagcgatc
      841 caccatcctc accctcctgc ggcggcggcg cgaactacaa cgatgtaagg acccagccta
      901 gagaccgcag aaccggacgt caacgaccac gggcgccggc tactcgaagt cctccgggct
      961 gacctggtcg aggaactcgc ggaacttctt gacctcccgc tcctcgtcgt cgctctcctt
     1021 gatgaggatg gacgcctcgc tgaggacgcc ctcctcggcg aagatggggg tgttggcgcg
     1081 gacggccagt gcgatggcgt cgctcggccg gctcgacacc gagaacctgg agccgttctg
     1141 gcgcagctcg atggtggcgt agaaggtgcc ttccttgagc tcggtgatca ccacccggtc
     1201 gatcgagacg accagctcgt cgagcaggtt cttgagcagg tcgtgggtca gggggcgggc
     1261 ggtggcgacg ccctggaggg cgaacgcgat ggccgtcgcc tccacgttgc cgatccagat
     1321 cggcaggaag cgctcgccgt cgcgctcctt gagcaggacg atcggcgtct gggtgggaag
     1381 ctcgatgcgg acgccgacga gggtcagctc gatcaaagcg gaaccccctc gagcgtggaa
     1441 gatacctgcg cccggccggg cgcctgcctg caatcagagg ctatcagacg cctcccccac
     1501 gcgcgacaag ccgaccgccg cgagcgcccc ggtcggggcg atcgtcaggg ccctccctgg
     1561 gtcaggaacg ggtcgaccac gggcgacgcc gacccggccg gcaccggggc ggcacggaag
     1621 cccccgccgc ccgagggcgc cgcgccgccg ttcggcccca gcggcagcgg cgcgagcagg
     1681 atgccccccg cggcgagcca cacgcgcggc ggggcgccgg tcccggcggc gtcggtggcg
     1741 ccagcccctg tggccccagc gggagcgggt ccgggccggg cgccgccgga cggcgcgggg
     1801 acggcggcga ccctggcgag caggtcgcgg cggacgaggg cgtccacgtc ggccccgacc
     1861 tgccagcggc cgccctgcac cgtcaccgtc cgcccgcggg cggccaggcg gacccgcacg
     1921 agggcgttga acccgtactc gaacagggcg cggacgtcgg tgaaggcgtt cggcgagccg
     1981 agggcgaccg cgaccagccg gcggccgccc cggcgggcgg acgcgaccag gcagcggcgg
     2041 gccaggttcg tgaacccggt cttgcctccc tcggtccagc ggtaggtgaa cagcaggtcg
     2101 ttgcggttgg ggaagcgccg cgagccggcc ggccccggga tcgtggccct cctggtgcc
//
LOCUS       DASPYY010000261         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_748562, whole genome shotgun sequence.
ACCESSION   DASPYY010000261 DASPYY010000000
VERSION     DASPYY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748562"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEP73_04000"
     CDS             complement(<1..194)
                     /locus_tag="VEP73_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HYT25632.1"
                     /translation="MPDAPLHDAVSGLRFDSRGLVPAVVQDDADGTVLMVAWMDAEAV
                     RRTLTTGRTWFWSRSRREHW"
     gene            366..>4250
                     /locus_tag="VEP73_04005"
     CDS             366..>4250
                     /locus_tag="VEP73_04005"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HYT25633.1"
                     /translation="MATKPEQAKAEILDRIVATVHERVPDEEVAQVEAFARRYYSRVA
                     PEDLLEQALPDLYGAVLAQWAFARRREEGASKVRVYSPRFEEHGWRSVHSIVEVISDD
                     MPFLVDSVTIEVNRRGLTIHLPIHPVLTVRRDAGGNLLEVLPEGAGAEDAIQESILHM
                     EIDRQTEPDVLEDLRAGVEHVLADVRAAVEDWPRMRERVADVLDAYTERPPPIDDVEV
                     EDTRALLRWIGEDHFTFLGYREYELVVVDGEDTLRSVPGTGLGILRDAGRAPVSVRFA
                     KLPPEVARLARARHPLVLTKANSRATVHRPSYLDYVGVKRFDEAGEVVGEWRFLGLYT
                     SAAYNRTPREIPVLRRKAEGVIERAAFPAGSHDEKALINILDTFPRDELFQIDEDELF
                     EVSMGILRLEERRRVRLFLWDDPYGRSLSCLVFVPRDRYNTEVRMRIEAVLQHAFPGA
                     TLDFSVLLSESVLARLHFVIRIPPGERPDFDVREIERQLVEATRSWRDELHGHLVEQH
                     GEELGNQLANRYADAFPAAYREDFPARSAVYDIRRMEELAEGGGLGMSLYRPLEAPGS
                     HLRFKLFWSERPLPLSDAVPMLEDMGVEVVEERPYEIRVPGRAPVWIHDFGLLHADGD
                     VDLHQVRDSFQEAFGRILRGEAESDGFNRLVLAAGLAWHQIVVLRAYCKYLRQTKLMF
                     SQAYMEETLAANPGVARGLVQLFEARLDPARARGGGQPSEEVERLSREVRAAIDSVAN
                     LDEDRILRSFLGLIEATLRTNHFQTGADGRRKPYLSFKLDPSKVPSLPAPRPMFEIFV
                     YSPRTEGVHLRGGRVARGGIRWSDRREDFRTEILGLMKAQMVKNAVIVPVGAKGGFVV
                     KRPPAERTREALADEVVACYSTLIRGMLDLTDNRVDGRVVPPPDTLRYDPDDPYLVVA
                     ADKGTATFSDIANSIAREYGFWLDDAFASGGSAGYDHKKMGITARGAWESVKRHFREL
                     GVDTQATDFTVVGVGDMSGDVFGNGMLLSRHTKLVGAFNHQHVLLDPDPDPEASFRER
                     ERLFALPRSSWADYDPAVISAGGGVFPRTAKSVPLSPEARRALGVDADALPTNELIQA
                     LLRAPVDLLWNGGIGTYVKSSRESHADVGDRTNDGVRVDAAELRCKVVGEGGNLGLTQ
                     LGRIEYALGGGRINTDFIDNSGGVDCSDHEVNIKILLNSVVAAGDMTRKQRDELLAAM
                     TDDVAESVLLDNYRQAQAISLTETQAPALLDEQARMMRSLERAGKLDRRLEFLPDREG
                     LAERRAAGLGLTRPELAVLLAYSKVTLEEELIA"
BASE COUNT          612 a         1520 c         1551 g          567 t
ORIGIN      
        1 tgccagtgct cccgccgcga gcggctccag aaccaggtcc ggccggtggt gagggttcgg
       61 cggacggcct ctgcgtccat ccaggcgacc atgaggacgg tgccgtcggc gtcgtcctgg
      121 acgaccgccg ggacgaggcc ccgcgagtcg aagcgcaggc cggagacggc gtcgtggagc
      181 ggggcgtcgg gcaaggaggc tcctctgggc gggcgggagg cgccgcctcc cgggtcgggg
      241 cggccggttg cgcgcccatg ctattcgcgg cccggccggt acggcacact agggccggcc
      301 tcgcatcgac gccgagcggc gggctcatcg gcctggccgc ggtggcaggc accgggaggg
      361 acctcatggc gaccaagccc gagcaggcga aggcggagat cctcgaccgc atcgtggcca
      421 cggtccacga gcgggtccct gacgaggagg tggcccaggt cgaggcgttc gcgcgccggt
      481 actacagccg ggtggccccc gaggacctgc tcgagcaggc cctgcccgac ctgtacgggg
      541 cggtcctcgc ccagtgggcc ttcgcccgcc gccgcgagga gggcgcctcg aaggtgcgcg
      601 tctacagccc ccggttcgag gagcacgggt ggcgctcggt ccactcgatc gtcgaggtga
      661 tctcggacga catgccgttc ctggtcgact cggtgaccat cgaggtgaac cgccgcgggc
      721 tcaccatcca cctgccgatc cacccggtcc tgaccgtccg ccgcgacgcc gggggcaacc
      781 tgctcgaggt gctgccagag ggcgccggcg ccgaggacgc gatccaggag tcgatcctgc
      841 acatggagat cgacaggcag accgagccgg acgtgctcga ggacctgcgg gccggcgtcg
      901 agcacgtcct ggccgacgtg cgcgccgcgg tcgaggactg gccgcggatg cgcgagcggg
      961 tggcggacgt gctcgacgcc tacaccgagc gcccgccccc gatcgacgac gtcgaggtcg
     1021 aggacaccag ggcgctgctg cgctggatcg gcgaggacca cttcaccttc ctcggctacc
     1081 gggagtacga gctcgtcgtg gtggacggcg aggacacgct gcgctcggtc ccgggcacgg
     1141 ggctcggcat cctgcgcgac gccgggcggg cgccggtctc ggtccgcttt gccaagctgc
     1201 cccccgaggt cgcgaggctc gcccgggcgc gccacccgct ggtgctgacc aaggccaact
     1261 cgcgggcgac cgtccaccgg ccgtcctacc tcgactacgt cggggtcaag cggttcgacg
     1321 aggccgggga ggtcgtcggc gagtggcgct tcctcggcct gtacacctcg gccgcctaca
     1381 accggacccc gcgcgagatc ccggtcctgc gccgcaaggc cgagggcgtg atcgagcggg
     1441 ccgcgttccc ggccggcagc cacgacgaga aggcgctcat caacatcctc gacaccttcc
     1501 cgcgcgacga gctgttccag atcgacgagg acgagctctt cgaggtgtcc atgggcatcc
     1561 tgcggctcga ggagcgcagg cgggtccgcc tgttcctgtg ggacgacccc tacgggcgct
     1621 cgctgtcctg cctggtcttc gtgcccaggg accgctacaa caccgaggtc cgcatgcgga
     1681 tcgaggcggt gctgcagcac gccttcccgg gcgcgaccct ggacttctcc gtcctgctgt
     1741 cggagtcggt gctcgcccgg ctgcacttcg tcatccgcat cccacccggc gagcggcccg
     1801 acttcgacgt gcgggagatc gagcggcagc tcgtcgaggc gacccgctcg tggcgcgacg
     1861 agctgcacgg ccacctggtc gagcagcacg gcgaggagct cggcaaccag ctcgcgaacc
     1921 ggtacgccga cgccttcccg gccgcctacc gggaggactt cccggcccgg agcgccgtct
     1981 acgacatccg gcggatggag gagctggccg agggcggcgg cctcggcatg agcctgtacc
     2041 ggccgctcga ggcgccaggc agccacctgc ggttcaagct gttctggtcg gagcgcccgc
     2101 tgcccctgtc cgacgccgtg ccgatgctcg aggacatggg cgtcgaggtg gtcgaggagc
     2161 ggccctacga gatccgcgtg cccggcaggg cgccggtgtg gatccacgac ttcggcctgc
     2221 tccacgccga cggggacgtc gacctgcacc aggtgcgcga cagcttccag gaggcgttcg
     2281 gacggatcct gcgcggcgag gccgagagcg acggcttcaa ccggctcgtg ctcgccgccg
     2341 ggctcgcctg gcaccagatc gtggtcctgc gcgcctactg caagtacctg cgccagacga
     2401 agctcatgtt cagccaggcg tacatggagg agaccctggc ggccaacccg ggcgtcgcgc
     2461 gcgggctcgt ccagctgttc gaggcccggc tggacccggc aagggcgcgc ggggggggcc
     2521 agccttccga ggaggtcgag cggctaagcc gcgaggtccg ggccgcgatc gactcggtgg
     2581 ccaacctcga cgaggaccgc atcctgcgca gcttcctcgg cctgatcgag gcgaccctgc
     2641 ggaccaacca cttccagacc ggcgcggacg gccggcgcaa gccctacctg tcgttcaagc
     2701 tcgacccgtc caaggtcccg agcctgccgg cgccgcggcc gatgttcgag atcttcgtgt
     2761 actcgccccg gaccgaaggg gtccacctgc gcggcgggcg ggtcgcgcgg ggcgggatcc
     2821 gctggtcgga ccggcgggag gacttccgca ccgagatcct cgggctgatg aaggcccaga
     2881 tggtgaagaa cgcggtgatc gtgccggtgg gcgccaaggg cgggttcgtg gtcaagcgcc
     2941 cgcccgccga gcgcaccagg gaggcgctcg ccgacgaggt cgtggcctgc tactcgacgc
     3001 tcatccgcgg catgctcgac ctgaccgaca accgggtcga cggccgggtc gtccccccgc
     3061 ccgacaccct ccgctacgac ccggacgacc cctacctggt ggtcgccgcc gacaagggca
     3121 cggcgacgtt ctccgacatc gccaactcga tcgcgcggga gtacgggttc tggctcgacg
     3181 acgcgttcgc gtcgggcggg tcggccggct acgaccacaa gaagatgggc atcaccgcgc
     3241 ggggcgcctg ggagtcggtc aagcggcact tccgcgagct gggcgtcgac acccaggcga
     3301 ccgacttcac ggtcgtcggc gtgggcgaca tgtcggggga cgtgttcggc aacgggatgc
     3361 tcctctccag gcacacgaag ctggtcggcg cgttcaacca ccagcacgtc ctcctcgacc
     3421 cggaccccga ccccgaggcg agcttccgcg agcgcgagcg cctgttcgcc ctcccccgct
     3481 cctcctgggc ggactacgac cccgcggtga tctcggccgg tggcggggtg ttcccgcgga
     3541 cggccaagtc ggttcccctg tcgccggagg cgaggcgggc gctcggcgtc gacgcggacg
     3601 cgctgccgac caacgagctg atccaggcgc tcctccgcgc cccggtcgac ctgctgtgga
     3661 acggcggcat cggcacctac gtgaagtcgt cgagggagag ccacgcggac gtcggcgacc
     3721 ggaccaacga cggcgtgcgg gtggacgccg ccgagctgcg ctgcaaggtc gtcggcgagg
     3781 gcggcaacct ggggctcacc cagctcggcc ggatcgagta cgcgctcggc gggggccgca
     3841 tcaacaccga cttcatcgac aactcgggcg gggtggactg ctccgaccac gaggtgaaca
     3901 tcaagatcct gctgaactcg gtggtcgcgg ccggcgacat gacccgcaag cagcgcgacg
     3961 agctgctcgc ggcgatgacc gacgacgtgg ccgagagcgt cctgctcgac aactaccggc
     4021 aggcacaggc gatcagcctg accgagacgc aggccccggc gctgctcgac gagcaggcgc
     4081 gcatgatgcg ctcgctggag cgcgccggca agctcgaccg gcggctcgag ttcctgcccg
     4141 accgggaggg gctggccgag cggcgcgccg ccgggctcgg gctcacccgc cccgagctgg
     4201 ccgtgctgct cgcctacagc aaggtgaccc tggaggagga gctgatcgcc
//
LOCUS       DASPYY010000262         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1171078, whole genome shotgun sequence.
ACCESSION   DASPYY010000262 DASPYY010000000
VERSION     DASPYY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171078"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..415
                     /locus_tag="VEP73_04010"
                     /pseudo
     CDS             152..415
                     /locus_tag="VEP73_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
     gene            complement(629..1840)
                     /locus_tag="VEP73_04015"
     CDS             complement(629..1840)
                     /locus_tag="VEP73_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYT25634.1"
                     /translation="MTVRQNAHLTFRENTAAGRHGWLRLTPAYSYRLVAETLGRREPD
                     TVVLDPFSGSGTTGLFAAQAGLAATLVDLNPFLVWLAGAKTRGYGAAELGDARDRAAR
                     VVGSARAAAGTEGLWQPPIFRIDRWWSPPALDGLRALRAALDEHAGAGPADDLLLVAL
                     CRTLIAVSRAAFDHQSMSFKQGGAAGSVAGVLERYRLEAEHVVATAAAPLPGRACVRL
                     GDARSLAGLVDRPAEVLYTSPPYANRVSYVRELRPYMYWLRFLGDARQAGELDWSAVG
                     GTWGVATSRLAGWSPAQAVPLDGSFTATLGRIATAEHASGRLLARYVERYFCDMWRHF
                     LSAHDVVAPGGTVVYVVGNSVFFGHVVPAQAWYAELLAEAGFTGVEVRTLRKRNSKAA
                     LYEYAVTARRG"
     gene            2058..>2370
                     /locus_tag="VEP73_04020"
     CDS             2058..>2370
                     /locus_tag="VEP73_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT25635.1"
                     /translation="METVETTRPAPLSETPDRYGAYPRLDDAQIQALAEQGERRRTLP
                     GDVLYREGDTHYDFVVILEGLVAVLEGCGSEEERVLGAHGPRRFMGELDLLTGEAAFV
                     TA"
BASE COUNT          323 a          848 c          892 g          307 t
ORIGIN      
        1 aggactgtct tggataaagc tcagcgcgtc cttcgagacg cccgctttgc gggctcctca
       61 agatgaggtt ctttcttaat tccaccaaga acttacatca tcctgagaag cgccccgcgg
      121 gagcgcatct cgaagggcgc atgacgtgac gatgagcgag gcgggcctcg gcgagttcgg
      181 ccgcatccgg cgcttctttg cgccgctcgc cgggccgggc gggcttggcc tcacggacga
      241 tgcggcactc atcgattgcc gccccggaca tcggctcgtc gtcacggtcg atgcgatcgt
      301 cgagggtgtg cattatttgc cggaggatcc gcccgacctc gtcgcccgca agctgttgcg
      361 gatgaatctg tcggacctcg ccgcgatggg cgcgcggctc cggcgcatgg tcctcgaccg
      421 tttcacatgg gaccgcgccg cgctcgagta cctctcgctc taccggcgcc tgctccagcg
      481 gcccggtccc ggccactcca tgccgaccgg ggaggatgga ccggttggca gctaggatgc
      541 gaccagcatc accccctccg ctgcgctctg ttcggccgct ctcccggcag gagcgtcaag
      601 ggcccatggt gggagtggtg ggggagtgct acccgcggcg cgcggtcact gcgtactcgt
      661 agagcgccgc cttcgagttc cgcttgcgca gggtccggac ctccacgccg gtgaagccgg
      721 cctcggcgag cagctcggcg taccacgcct gcgccggcac cacgtgcccg aagaacacgg
      781 agttgccgac gacgtagacg accgtcccgc cgggcgcgac cacgtcgtgg gcggacagga
      841 agtgccgcca catgtcgcag aagtaccgct ccacgtagcg ggcgagcagc cgcccgctcg
      901 cgtgctcggc cgtcgcgatg cgcccgagcg tcgccgtgaa ggacccgtcg agggggaccg
      961 cctgcgccgg cgaccacccg gcgagccggc tggtggcgac gccccaggtg cccccgaccg
     1021 cgctccagtc gagctcgccg gcctgcctcg cgtcgccgag gaagcgtagc cagtacatgt
     1081 aggggcgcag ctcgcggacg tagctgaccc ggttggcgta tgggggcgag gtgtagagga
     1141 cctcggcggg gcggtcgacc aggccggcga gcgagcgcgc gtcgccgagc cgcacgcagg
     1201 cccggccggg caggggggcg gcggcggtgg cgacgacgtg ctccgcctcg agccggtagc
     1261 gctccagcac gccggcgacg ctcccggccg cgccgccctg cttgaacgac atcgactggt
     1321 ggtcgaacgc ggcgcggctc acggcgatga gggtgcggca gagcgccacg agcagcaggt
     1381 cgtccgctgg gccggcgccg gcgtgctcgt cgagggcggc ccggagcgct ctgagcccgt
     1441 cgagggccgg cggggaccac cagcggtcga tccggaagat cggtggctgc cagaggccct
     1501 ccgtgcccgc ggcggccctg gccgagccga ccacccgcgc cgcccggtcg cgcgcgtcgc
     1561 cgagctccgc cgcgccgtag ccgcgcgtct tggcccccgc gagccacacc aggaacgggt
     1621 tgaggtcgac cagggtcgcg gcgaggcccg cctgggcggc gaacagcccg gtcgtgccgc
     1681 tccccgagaa cgggtcgagc acgacggtgt ccggctcgcg ccgcccgagc gtctcggcga
     1741 cgagccggta ggagtaggcc ggggtcaggc gcagccagcc gtgcctgccc gccgccgtgt
     1801 tctccctgaa ggtcaggtgg gcgttctgcc ggacggtcat gggcgagggt cccgccggtg
     1861 acggcgggcc ggccgggtga cggacagtct cccacgcccg cgcggctcgg ggcggcggga
     1921 gggacgacga gccgggccga gcgactaccc gggcgaaacg gtgcaactca acagcccgcg
     1981 cagggatgcg gccggccgcc gccggccgcc gccgccggcc gccaaggtgg cggtcgtgac
     2041 cacgacggag gtgccgggtg gaaacggtgg agacgacacg gccggcgccg ctgtccgaga
     2101 cgcccgaccg ctacggggcg tacccgcggc tcgacgacgc gcagatccag gccctcgccg
     2161 agcagggcga gcggcggcgc accctccccg gcgacgtgct gtaccgggag ggcgacacgc
     2221 actacgactt cgtcgtgatc ctggaaggcc tggtggcggt cctcgagggg tgcgggagcg
     2281 aggaggagcg cgtgctcggt gcccacggcc cccgccgctt catgggcgag ctcgacctgc
     2341 tcaccggcga ggccgcgttc gtcaccgcga
//
LOCUS       DASPYY010000263         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1311087, whole genome shotgun sequence.
ACCESSION   DASPYY010000263 DASPYY010000000
VERSION     DASPYY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311087"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VEP73_04025"
     CDS             <1..570
                     /locus_tag="VEP73_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYT25636.1"
                     /translation="QGFEPAEPGADFERLSSSIEQVSDVLGDQPLEFYERDLPQIAAA
                     ETARRVRADVVVLLLDNGEGLLEVSGGVGLTPAERRMNVDYNRDVMRELFRAGVGLVE
                     DTDRVRGALAGIPGSRAETLVMVPLVHDRLGFGVLVAGRRRSPNGLETAVFTDQEVEA
                     LMSFADDAAPSLRTAVLLRHLKRQLKPIE"
     gene            complement(616..>1587)
                     /locus_tag="VEP73_04030"
     CDS             complement(616..>1587)
                     /locus_tag="VEP73_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25637.1"
                     /translation="RRRPLALAGVPLLMVAWANTHGAFVVGLALVAVEAVVAACRRDG
                     RTAARLGLALAAGVAALLANPWGAGVLRYAVALPTNPHVTAGVTEWAPPSVRDPAGAA
                     LFAVLAVLVVALARARAPARAAGQVARIVPLTALALWTTRAEVWWGLALPLVLVSLAR
                     GQEPRTPGADRGTPLLTGSILLLLALAMAAVAPGTRSVLVPASRSAPRLTAAPAATAD
                     WLAAHPQPGRMLNFQPWGSYLEFRLGPAVKPAVDSRIELTPAAFWDDYYAIVSGRWDT
                     QRLLDRHAVSYVVTDARKTPDLVAGLEASGGWRLAFRSRDERVFVRR"
BASE COUNT          225 a          580 c          612 g          170 t
ORIGIN      
        1 cagggcttcg agccggccga gccgggggcc gacttcgagc ggctatcgag ctccatcgag
       61 caggtctccg acgtcctggg tgaccagccg ctcgagttct acgagcggga cctgccccag
      121 atcgcggcgg ccgagaccgc gagacgcgtg cgtgccgacg tcgtcgtcct cctgctcgac
      181 aacggtgagg gactgctcga ggtctcgggc ggggtggggc tgacccctgc ggagcggcgc
      241 atgaacgtgg actacaaccg ggacgtcatg cgggagctgt tccgggccgg ggtcgggctc
      301 gtcgaggaca ccgaccgcgt caggggcgcc ttggccggga tccccggcag ccgggccgag
      361 accctcgtca tggtgccgct ggtgcacgac cggctcgggt tcggggtgct cgtcgccggc
      421 cggcgccgca gccccaacgg cctcgagacc gccgtcttca ccgaccagga ggtggaggcg
      481 ctgatgagct tcgccgacga cgcggcgccc tccctgagga ccgccgtgct cctccgccac
      541 ctcaagcgcc agttgaagcc gatcgagtga gccggcgccc gggaggacgc gcgggccgcg
      601 cccgcccggc ccggttcagc gccgcacgaa cacgcgctcg tcgcggctcc ggaacgcgag
      661 ccgccagcca ccagacgcct cgaggccggc gaccaggtca ggcgtcttgc gcgcgtcggt
      721 gacgacgtag gacacggcat gccggtcgag cagccgttgc gtgtcccacc gaccgctcac
      781 gatcgcgtag tagtcgtccc agaacgcggc cggcgtcagc tcgatacgcg agtcgaccgc
      841 cggcttgacc gccgggccga ggcgaaactc gaggtaggag ccccacggct ggaagttgag
      901 catgcggccg ggctgcgggt gggccgccag ccagtcggcg gtcgcggccg gggcggcggt
      961 cagccggggc gccgaccggc tcgcaggcac gagcacggag cgggttcccg gtgcgacagc
     1021 cgccatcgcg agggcgagga gcagcaggat cgacccggtg agcagcggcg ttccccggtc
     1081 ggcgcctggc gtccgaggct cctggccgcg cgccagggag acgaggacga gcgggagcgc
     1141 cagcccccac cagacctcgg cccgggtcgt ccacagggcg agggcggtga gcgggacgat
     1201 ccgggcgacc tgcccggcgg cgcgagccgg agcccgggcc cttgcgagcg cgacgaccag
     1261 gaccgcgagg acggcgaaca gggcggcacc ggcgggatcg cgcacgctcg ggggcgccca
     1321 ctctgtcacc ccggccgtga cgtgggggtt ggtgggcagc gcgaccgcgt agcggagcac
     1381 gcccgcaccc cacgggttcg cgagcagggc ggccacgcca gccgccagcg cgaggccgag
     1441 gcgtgccgcc gtccgcccgt cgcgcctgca tgcggcgacc accgcctcga cggcgacgag
     1501 cgccagcccg acgacgaacg cgccatgcgt gttcgcccag gccaccatga gcaacgggac
     1561 gcccgcgagc gccaggggcc gccgcct
//
LOCUS       DASPYY010000264         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1732105, whole genome shotgun sequence.
ACCESSION   DASPYY010000264 DASPYY010000000
VERSION     DASPYY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732105"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..>1733)
                     /locus_tag="VEP73_04035"
     CDS             complement(326..>1733)
                     /locus_tag="VEP73_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYT25638.1"
                     /translation="PAHDERDYDFAQAFNLPIRRVVGGHHDELPYMGDGPLVNSSPEF
                     DGLHNRDALEKIVDWLDRDGKGHRSINYRLRDWLLSRQRYWGCPIPIVHCEACGEVGV
                     PDDQLPVLLPADVTDFKPKGRSPLATSEAFVNVTCPSCGGPARRETDTMDTFVDSSWY
                     FLRYTGLDPDRPFDPERVARWMPVPQYTGGIEHAILHLLYSRFFVKVLHDLGLVPFTE
                     PFRALLNQGMVVMRGAAMSKSRGNLVEPSALIEEHGADVARLTMLFAGPFEDDVDWAD
                     VSPEGVARWVQRVWRTVHLAVGEPGAGAPDPELVRLAHRTTQGVGDDLERFRFNTAIA
                     KLMVLTNGLSDALRHGRGTAAERRDAAERLVLLLAPLAPFVTEELWRVLGGSTSVHLA
                     SWPAYDPGLVRTERVTCVLQVDGKVRDRIEVAPDTAGDALRAAALDSAKVRAALGDRA
                     VARVVVVPPKLVNVVTAR"
BASE COUNT          233 a          595 c          704 g          201 t
ORIGIN      
        1 ggcagcgccg cggacccaca gggcgcccgc gccggcgagc gcgaccagga cgacgagcgc
       61 gacggcggcg ctctggcggc cgacgcccgc gagcagcccg gcgagccggg cgcgccacgg
      121 gtcggggggc aggacgaacg ggggctggga cggcatgccg gtcctcccgg gggctgccgg
      181 gccgctgagg cggcccggag gggcgatcgc tgacaccccg accgtagccg gccgcggcgg
      241 cgccgtcccc gcgccgtcca cacccccggg cgacccactg gggccgggcg ggcccggccg
      301 cccggcgccc ctcgcagggc gcgtactacc tggcggtgac cacgttgacg agcttcgggg
      361 gcacgacgac gaccctggcg accgcccggt cccccagggc ggcccggacc ttggcggagt
      421 cgagcgcggc cgcgcgcagg gcgtccccgg cggtgtcggg cgcgacctcg atccggtcgc
      481 gcaccttgcc gtcgacctgc aggacgcagg tgacgcgctc ggtgcggacc aggccggggt
      541 cgtaggcggg ccagctcgcc aggtggaccg acgtggagcc gccgagcacc cgccacagct
      601 cctcggtcac gaacggggcc aggggggcga gcaggaggac gagccgctcg gcggcgtcgc
      661 gccgctccgc ggcggtcccc cgcccgtggc ggagcgcgtc ggagaggccg ttggtgagca
      721 ccatcagctt cgcgatggcg gtgttgaagc ggaagcgctc gaggtcgtcg ccgaccccct
      781 gggtggtgcg gtgggcgagc cggacgagct ccgggtcggg cgctccggcc ccgggctcgc
      841 cgaccgcgag gtggaccgtg cgccagaccc gctggaccca gcgcgcgacg ccctcggggg
      901 acacgtcggc ccagtccacg tcgtcctcga aggggccggc gaacagcatc gtcaggcggg
      961 cgacgtcggc gccgtgctcc tcgatgagcg ccgacggctc gaccaggttg ccgcgcgact
     1021 tgctcatggc ggcaccgcgc atgacgacca tgccctggtt gaggagggcg cggaacggct
     1081 cggtgaacgg gaccagcccg aggtcgtgca gcaccttgac gaagaagcgg gagtagagca
     1141 ggtgcaggat ggcgtgctcg atcccgccgg tgtactgggg caccggcatc cacctggcca
     1201 cccgctcggg gtcgaacggg cggtcggggt cgagcccggt gtagcggagg aagtaccagg
     1261 acgagtcgac gaacgtgtcc atggtgtcgg tctcgcgcct ggcggggccg ccgcaggagg
     1321 ggcaggtcac gttgacgaag gcctcggagg tggcgagcgg cgagcgcccc ttgggcttga
     1381 agtcggtgac gtcggcgggc aggaggacgg gaagctggtc gtcgggcacg ccgacctcgc
     1441 cgcaggcctc gcagtggacg atggggatcg ggcagcccca gtagcgctgg cgggacagca
     1501 gccagtcacg caggcggtag ttgatcgagc ggtggccctt gccgtcgcga tcgagccagt
     1561 cgacgatctt ctccagcgcg tcgcggttgt gcaggccgtc gaactccggc gaggagttca
     1621 cgagcgggcc gtcgcccatg tacggcagct cgtcgtggtg gccgccgacg acgcggcgga
     1681 tcggcaggtt gaaggcctgc gcgaagtcgt agtcgcgctc gtcgtgcgcc ggt
//
LOCUS       DASPYY010000265         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_748633, whole genome shotgun sequence.
ACCESSION   DASPYY010000265 DASPYY010000000
VERSION     DASPYY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748633"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VEP73_04040"
     CDS             <1..1520
                     /locus_tag="VEP73_04040"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HYT25639.1"
                     /translation="FVPGLVSEGATVVTAAAGVGLVVLAGGLRRRHRAAWLATIALLA
                     ASAVLHLAKGLDYEEALIEAFLAGLLLATGEQFAARLGPRERRAVVGPVLGVAAVTAG
                     YGILGLVTARSVAVESLSVVATLREVGRMALGLGASLTLTGRFGAFFPASVAAVFYVG
                     MLAAAARVLAPALARRVADPGLAAAVAASDDSLAYFALRDDRATVRAGDAVVAYGTVG
                     TVALAAGDPLGPWTQWPRAVDAFMAEAAAQGRIAAVLGCGAEAARVYAAAGLHVAYLG
                     DEALLDLERFTLDGRALRIARQSWNRARRAGFESVVCRSSELAPDAVASLRELSERWR
                     GGAAERGFSMALGRLFDPRDGQTLVVAARDADGRPQGFLHFVPWGADGASLDVMRRDP
                     EAPAVLNDFLVVEAARRLPEVGVRRLSLNFSFLRAVLAAGAEPDVPVRLRAVRWLLRR
                     LSHPFQIETLYRFNKKFGPTWHPRYVAVEVLEELPRIALAALRAEGLLQPPWRLR"
     gene            1517..>3358
                     /locus_tag="VEP73_04045"
     CDS             1517..>3358
                     /locus_tag="VEP73_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYT25640.1"
                     /translation="MTHALGKARSVLELGTVVLGMALLASCCLVPVLVPAGRLVTRTT
                     GQYGDIPPLGRGLRRPAERSVVRAADGSVLGTLHLDQDRQVVPLRAIPPMVRQAVLAV
                     EDARFYEHGALDYRGIARAILVDVGHGRIHEGGSTLTQQYVKNVVTGDQVSLRRKLAE
                     GIYASQLERRVTKDQILEAYLNEVYFGQGVYGIATAAEHYFSRPVGRLTLPEAAALAG
                     TIAAPERLNPTGGRPALERRDVVLDRMAAVGFAPRSLVAAAERQPLRPRVQQPVTRAP
                     YFVDYVERQLQDDHAFDDALGPAGSAARRRAVFEGGLSISTTLDPRDQRLAEQAVTGQ
                     VGPSGLGAAVVSVDPATGGVLAMLGGRDFSASKVNLATGQGGSGGFPPGSSFKVFYLV
                     AALEQGFAPSTRFDTASPTTVSAPECPDGYTVHNAEGNGLGRIDLRRATAASVNTYYA
                     QLMARVGTPDAIDAARRMGITTPLRDYCSLVLGTENVTPLELASAYATLADGGVHCQP
                     TAIDRITDASGRPLWARSASCRGVLDPGIAAGAVDILRGVVAKGGTGYRAAIGRPLAG
                     KTGTTSNFENAWFTGFTPRLSTSVWVGEPTRQQPMTSQFNGGPVYGGT"
BASE COUNT          368 a         1316 c         1221 g          453 t
ORIGIN      
        1 gcttcgtgcc cggcctggtc tccgagggcg ccacggtcgt caccgcggcg gccggcgtcg
       61 gcctcgtggt gctcgccggc ggcctccggc ggcggcaccg cgcggcatgg ctcgcgacca
      121 tcgccctgct ggccgccagc gcggtgctgc acctggcgaa gggcctcgac tacgaggagg
      181 cgctgatcga ggccttcctc gccgggctgc tcctcgccac gggcgagcag ttcgccgccc
      241 gcctcggccc gcgtgagcgg cgggccgtgg tcgggcccgt cctcggcgtc gccgcggtca
      301 ccgccggcta cggcatcctc ggcctggtga ccgccaggag cgtggcggtg gagagcctgt
      361 ccgtggtggc caccctccgg gaggtcgggc ggatggcgct cggtctcggg gcctcgctca
      421 ccctcaccgg ccgcttcggc gccttctttc ccgcctcggt ggccgccgtc ttctacgtcg
      481 gcatgctcgc cgccgcggcc cgtgtcctcg cgccggcgct cgcccgccgg gtcgccgacc
      541 cgggcctcgc cgcggccgtc gccgcgtcgg acgactcgct ggcgtacttc gccctccgcg
      601 acgaccgggc caccgtccgg gccggcgacg cggtcgtcgc ctacggcacc gtcggcacgg
      661 tggcgctcgc cgccggggac ccgcttgggc cctggacgca gtggccgcgt gcggtcgacg
      721 ccttcatggc cgaggcggcc gcccagggcc gcatcgcggc cgtgctcggc tgcggcgccg
      781 aggcggcccg cgtctacgcg gcggccgggc tgcacgtggc ctacctcggt gacgaagccc
      841 tgctcgacct cgagcgcttc acgctcgacg gccgggcact gcggatcgcg cgccagagct
      901 ggaaccgggc gcgccgggcc gggttcgagt cggtggtctg ccggtccagc gagctggcgc
      961 ccgacgcggt cgccagcctc cgcgagctgt cggagcgctg gcggggcggg gccgccgagc
     1021 gcggcttctc gatggcgctc ggccggctct tcgacccccg cgacggccag accctcgtgg
     1081 tcgccgcccg cgacgccgac gggcggccgc agggcttcct ccacttcgtc ccctggggcg
     1141 ccgacggcgc gagcctggac gtcatgcggc gcgaccccga ggcgccggcc gtcctcaacg
     1201 acttcctggt cgtcgaggcg gcccgccggc tgccggaggt cggggtacgc cggctgtcgc
     1261 tcaacttctc cttcctgcgc gcggtgctcg cggccggtgc cgagcccgac gtccccgtgc
     1321 ggctgcgtgc cgtccgctgg ctgctgcgcc ggctgtcgca cccgttccag atcgagaccc
     1381 tctaccgctt caacaagaag ttcgggccca cctggcaccc ccgctacgtc gccgtcgagg
     1441 tgctcgagga gctgccccgc atcgccctgg cggccctcag ggcagagggc ctgctgcagc
     1501 cgccctggcg gctccgatga cgcacgcgct cggcaaggcg cgctccgtcc tggagctggg
     1561 cacggtggtg ctcggcatgg cgctgctggc gagctgctgc ctggtgcccg tgctcgtccc
     1621 cgccggccgg ctcgtcacgc gcaccaccgg gcagtacggc gacatcccgc cgctcggccg
     1681 cggcctgcgg cggccggcgg agcggtcggt cgtccgcgcg gccgacgggt cggtgctcgg
     1741 cacgctccac ctcgaccagg accgccaggt cgtgcccttg cgggccatcc cgccgatggt
     1801 caggcaggcg gtcctcgccg tcgaggacgc acggttctac gagcacggcg cgctggacta
     1861 ccggggcatc gcgcgcgcga tcctcgtcga cgtgggtcac gggaggatcc acgagggcgg
     1921 cagcaccctg acccagcagt acgtgaagaa cgtcgtgacc ggcgaccagg tgagcctccg
     1981 ccgcaagctg gccgagggga tctacgcctc gcagctcgag cggcgcgtga ccaaggacca
     2041 gatcctcgag gcctacctca acgaggtgta cttcggccag ggcgtctacg ggatcgccac
     2101 cgcggccgag cactacttct ccaggccggt cggcaggctg accctgccag aggccgccgc
     2161 cctggccggc accatcgccg ccccggagcg gctcaacccg accggcggcc ggccggccct
     2221 cgagcggcgg gacgtggtcc tcgaccgcat ggccgcggtc gggttcgccc cccggtccct
     2281 ggtcgcggcg gccgagcggc agccgctgcg gccacgggtg cagcaaccgg tgacccgcgc
     2341 gccctacttc gtcgactacg tcgagcgcca gctccaggac gaccatgcct tcgacgacgc
     2401 gctcgggccg gcgggctcgg ccgcccgcag gcgggcggtc ttcgaggggg gtctgtccat
     2461 ctcgaccact ctggaccccc gcgaccagcg gctcgccgag caggcggtca ccggccaggt
     2521 gggcccgagc ggcctcggtg ccgccgtggt cagcgtcgac ccggcgaccg ggggggtcct
     2581 ggccatgctg ggcggccgcg acttctcggc gtccaaggtc aacctcgcga ccgggcaggg
     2641 cggctcgggc gggtttccgc ccggctcctc gttcaaggtc ttctacctcg tcgcggcgct
     2701 cgagcagggg ttcgcaccga gcacccggtt cgacaccgcc tcgccgacca cggtgagcgc
     2761 ccccgagtgc ccggacgggt acacggtcca caacgccgag ggcaacgggc ttggccgcat
     2821 cgacctgcgc cgggccaccg cggcgtcggt gaacacctac tatgcgcagc tcatggcccg
     2881 ggtgggcacc ccggacgcga tcgacgcggc caggcgcatg ggcatcacca ctcccctgcg
     2941 cgactactgc tcgctcgtgc tcggcaccga gaacgtcacc ccgctcgagc tcgcctcggc
     3001 ctacgcgacc ctcgcggacg gcggggtcca ctgccagccg accgccatcg accgcatcac
     3061 cgacgcgagc ggccggccgc tctgggccag gagcgcctcg tgccgggggg tcctggatcc
     3121 cggcatcgcg gcgggcgcgg tcgacatcct ccgtggcgtg gtggcgaagg gcgggaccgg
     3181 gtaccgcgcg gccatcggcc ggcccctggc cggcaagacc ggcacgacca gcaacttcga
     3241 gaacgcctgg ttcaccgggt tcacgccgcg gctctcgacc tcggtctggg tcggcgagcc
     3301 gacgcgccag cagccgatga ccagccagtt caacggcggc cctgtctacg gcggcacc
//
LOCUS       DASPYY010000266         5292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1030840, whole genome shotgun sequence.
ACCESSION   DASPYY010000266 DASPYY010000000
VERSION     DASPYY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030840"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..423)
                     /locus_tag="VEP73_04050"
     CDS             complement(10..423)
                     /locus_tag="VEP73_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25641.1"
                     /translation="MPDPDTDTAPRLRVLVVDDCPQLRRHVREALEDAGVVVVGEAAD
                     GVQALSEAAARRPDVVLMDLRMPRMGGVEAALTLRRRQPEMRVVLWTGEDDERLASAV
                     RRSGADAGLAKGVRINELVATLRAVCAAQPIHASR"
     gene            607..861
                     /locus_tag="VEP73_04055"
     CDS             607..861
                     /locus_tag="VEP73_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HYT25642.1"
                     /translation="MEETYNGMSALHLRGVQWCKGSRSSGQGNCVETAKLPGGGAAVR
                     NSRHPEGPALIFTAGEIQAFLASVKLGDFDDLLHACSEQA"
     gene            1147..3081
                     /locus_tag="VEP73_04060"
     CDS             1147..3081
                     /locus_tag="VEP73_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase family protein"
                     /protein_id="HYT25643.1"
                     /translation="MADVTAGLLDPLLAVDAPPFALLRREGAPGVELLLGELVEVERL
                     ADIPLPGRRPGAAGQDVLAAVPFRQVTERGFACRDDRAPLRCLLVERAALLDPAEVAS
                     RLPDGPVVATGGRFDVDDEAYAATVKRVVADEIGRGEGANFVIRRDFRCRLAGPASRA
                     ALTLFRRLLAAEHGAYWTFVVHAGDLTMVGATPERHVTVHGDRMLMNPISGTYRYPPG
                     GPTVEGLLAFLADRKEVEELYMVLDEELKQMSAAGDLGGQVLGPYLKEMGHLAHTGYL
                     LAGRSSLDVRDILRATMFAATVTGSPVENACRVIAAHEATGRGYYGGVLALVGQDGQG
                     RRTMDAPILIRTLYLRPDGDGLQATLPVGATLVRHSDPAGEVAELHAKAAGSLAAIGL
                     RGQRAGRPETRLAGRERAPGRSGPGLAGDPRVAAALAGRNAPLAPFWLEPQDAAGVLA
                     PELAGRTALVVDAEDAWTEMLAHMLRRLGLAVTVRRWSETLVGEDLVGEDHDLLVAGP
                     GPGDPRELAQPRMAALRELLLARLEARRPLLAVCLSHQLLGGLLGLPLARKDVPCQGT
                     QREVDLFGRRERAGFYNSFAVRVPRAAPDGVDVAGDPLTGEVDALRGACFASTQFHLE
                     SVLTTNGVHVLRWLLEPLLG"
     gene            3304..3969
                     /locus_tag="VEP73_04065"
     CDS             3304..3969
                     /locus_tag="VEP73_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25644.1"
                     /translation="MAERATRLLSSAAPLDAALLATAVVGGPRQEVLDPSALDSTELC
                     SWLREATSTGANRVLDPALGTNVVLLPDAIELRQGPSGLRVDVAGGVRVLELVRVAGG
                     RPGRRTVDWADLRDGLHRALRLAAQVLDRIDPAGRLSQVAAAASVVGADVGRRPAVLP
                     RSSVGVVALGYGPARTVATLTPPVRAREALRSGQLAEELSERLGMQHDGPRELLPDAT
                     AAG"
     gene            4019..5200
                     /locus_tag="VEP73_04070"
     CDS             4019..5200
                     /locus_tag="VEP73_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYT25645.1"
                     /translation="MTSLGSEWSRDGPPTRTRRRARARAWRRRLVAALAIVLAVGLVA
                     VAGGYGYARFRFAQISSVRLPGLARRTAGTGAPFNVLVVGSDTRADLRPGEAAAYGSS
                     TAVTGQRSDVMMVVHLDPGTGRASILSIPRDLLVPIAGTGRRDRINAAFSGGPGRLVA
                     TIQGGLGIPIHHYLLVNFDGFQAIVNALGGIDLDFPYRSRDAYSGLDVTRAGCQHLGG
                     HAALALARSRHFEYWKDGSWHVDPLSDLSRIRRQQTFLRAVVARARSRGLTDPLAANA
                     FVGAVVHDITKDSALSVGEAVGLVSRFRSLDSKTLASYTLPTTAATGYPGYGDVLLLR
                     QAAARQVVARFLGRPATVSQAGAGGHEASVGGTTAPRPSSAPAPALPATAVPGFDPRP
                     C"
BASE COUNT          630 a         1993 c         1973 g          696 t
ORIGIN      
        1 ttcagcggct cagcgcgacg cgtggatggg ctgggccgcg cagaccgcgc gcagggtggc
       61 gaccagctcg ttgatgcgca cccctttggc gaggccggcg tccgcccccg accggcggac
      121 cgcgctggcg agccgttcgt cgtcctcccc ggtccacagc acgacgcgca tctccggctg
      181 ccgccgccgc agggtcaggg cggcctcgac gccgcccatc cgcggcatcc gcaggtccat
      241 caggaccacg tcgggacggc gcgccgccgc ctcggacagg gcctggacgc cgtcggcggc
      301 ctcgccgacc accaccaccc cggcatcctc gagtgcctcg cggacgtgcc ggcgcagctg
      361 cgggcagtcg tcgacgacca gcacccgcag ccggggagcc gtgtcggtgt ccgggtctgg
      421 catcgcgatc ctcgtggaga ctaggtgact cgttatgggt agcattcctg atgccaagtg
      481 cgaacgcaac tggcatctgc acgtgctaac gtcaagtgca cgtcatggtg tgggaaactg
      541 gcaacggcac cgggccagcc ggcaagacgg tgcagaccat caacacgatg ctgcgggaag
      601 ggtgacgtgg aagagaccta caacggcatg tccgccctgc acctgcgggg ggtccagtgg
      661 tgcaagggaa gccggagcag cggccagggc aactgcgtcg agacggcgaa gcttccaggc
      721 ggcggcgccg cggttcgcaa ctccagacat ccggaggggc cggcgctgat cttcaccgcc
      781 ggcgagatcc aggcgttcct cgccagcgtg aagctgggcg acttcgacga cctgctgcac
      841 gcctgctccg agcaggcatg atcccccgtg tgcccgggag ctgaaccggc ggaggcgatg
      901 tgacggacga gagccgggga cgtgccgaga cccacctcgc gccgcggctg gttgacaagc
      961 cgcccggccg cccctagcct cccggccatg cgcgcgaacc gtcttcgctg gtggtggctg
     1021 ccttgacggc ggcctcctga tcgcgcactc ctgatcgatg cggccgcctc gccgaggcgg
     1081 ccgcaggcgt tcaaccggct cgcagccttc cctcggcggg gtgccgcccg accgaaggga
     1141 aggcccatgg ccgacgtgac cgccgggctg ctcgacccgc tgctcgcggt ggacgccccg
     1201 ccgttcgcgc tgctgcggcg cgagggcgcg ccaggcgtgg agctgctgct cggcgagctg
     1261 gtcgaggtgg agcggctcgc cgacatcccg ctgcccggcc ggcggcctgg cgcggccggc
     1321 caggacgtgc tcgccgccgt cccgttccgc caggtgaccg agcgcggctt cgcctgccgc
     1381 gacgaccggg cgccgctgcg ctgcctcctg gtggagcgcg cggcgctcct cgaccccgcg
     1441 gaggtggcga gccgcctgcc ggacgggccg gtcgtggcga ccggcgggcg gttcgacgtc
     1501 gacgacgagg cctacgccgc cacggtcaag cgggtggtcg ccgacgagat cggccgtggc
     1561 gagggtgcca acttcgtcat ccgccgcgac ttccgctgcc gcctcgccgg cccggccagc
     1621 cgtgcggcgc tgaccctgtt ccgccgcctg ctcgcggccg agcacggcgc ctactggacg
     1681 ttcgtggtcc acgccgggga cctgaccatg gtcggcgcca cgccggagcg gcacgtcacc
     1741 gtgcacggcg accgcatgct gatgaacccg atcagcggca cctaccgcta cccgcccggc
     1801 ggcccgacgg tggagggcct gctcgcgttc ctggccgacc gcaaggaggt cgaggagctg
     1861 tacatggtcc tcgacgagga gctcaagcag atgagcgccg ccggcgacct cggcgggcag
     1921 gtcctcggcc cgtacctcaa ggagatgggt cacctggccc acaccgggta cctcctggcc
     1981 ggccgctcca gcctcgacgt gcgcgacatc ctgcgcgcga ccatgttcgc cgcgaccgtg
     2041 accgggtcgc cggtcgagaa cgcctgccgc gtgatcgccg cgcacgaggc gacggggcgc
     2101 ggctactacg gcggcgtgct cgcgctcgtc gggcaggacg gacaggggcg ccgcaccatg
     2161 gacgccccga tcctgatccg caccctgtac ctccgcccgg acggggacgg gctgcaggcc
     2221 acgctccccg tcggggcgac cctggtccgc cactccgacc cggccggcga ggtcgccgag
     2281 ctccacgcca aggcggccgg gtcgctggcc gcgatcgggc tgcgcgggca gcgggcaggg
     2341 cggcccgaga cccggttggc gggacgtgag cgtgcgcccg ggcgctccgg acccggcctc
     2401 gccggcgacc cgcgggtggc cgcggcgctc gcgggtcgca acgccccgct ggcgcccttc
     2461 tggctcgagc cgcaggacgc cgccggcgtg ctcgcgccgg agctggccgg ccggaccgcg
     2521 ctggtcgtcg acgcggagga cgcgtggacg gagatgctcg cgcacatgct gcgacggctc
     2581 ggcctcgccg tgacggtgcg gcgctggagc gagaccctcg tcggggagga cctcgtcggg
     2641 gaggaccacg acctgctcgt cgccgggcct ggcccgggcg acccgcgcga gctggcgcag
     2701 cccaggatgg ccgcgttgcg cgagctgctg ctcgcccgcc tggaggcgcg ccgcccgctg
     2761 ctcgccgtgt gcctgagcca ccagctcctc ggcggcctgc tcggcctgcc gctcgcccgg
     2821 aaggacgtgc cctgccaggg cacccagcgg gaggtggacc tgttcgggcg gcgcgagcgc
     2881 gccggcttct acaactcgtt cgcggtgcgc gtgccccggg ccgcgcccga cggcgtcgac
     2941 gtcgccggcg acccgctgac cggggaggtc gacgccctgc gcggcgcatg cttcgcgagc
     3001 acccagttcc acctcgagtc cgtcctcacc accaacggcg tgcacgtcct ccgctggctc
     3061 ctcgagccac tgctcggctg agccagcggg ctcgcccgcc cggtcgcgcc ctgacctcgg
     3121 gcctggctca tgctgttcta atgcctggtc gcgcacgatg ggcctgcggg agccggcggg
     3181 ggccggcccc cgggcaacga gctcgtgatg ccgtcgatgg ccacagcggc gccggcgcgg
     3241 ctcgcctgtg caagggaggg cgggttggtg aacagcgaca gcgggtcggg ggtgaggctg
     3301 gagttggccg agagggcgac caggctgctg tcgtcggcgg cgccgctcga cgccgcgctg
     3361 ctcgccacgg cggtggtggg cgggccacga caagaggtcc tcgacccgtc cgcgctcgac
     3421 tccacggagc tgtgcagctg gctccgcgag gcgacgtcga ccggtgcgaa ccgggtgctc
     3481 gacccggcgc tgggcaccaa cgtggtgctc cttcccgatg cgatcgagct ccgccagggc
     3541 ccttcgggac tccgcgtcga cgtcgccggc ggggtgcgcg tcctcgagct ggtccgggtc
     3601 gccggaggtc ggcctggccg gcgcacggtc gactgggcgg atctgcgcga cgggctccac
     3661 cgggcgctcc gcctggccgc gcaggtgctc gaccggatcg atcctgccgg ccgcttgtcg
     3721 caggtcgcgg cggctgccag cgtcgtcggc gccgatgtcg ggagacggcc ggccgtgctg
     3781 ccgcgctcct cggtcggcgt ggttgcgctc gggtacgggc cggcgcgcac cgtcgcgacg
     3841 ctcacgcctc cggtcagggc gcgggaggcg ctccgctcgg gccagctcgc cgaggagctg
     3901 agcgagcggc tcgggatgca gcacgatggc ccgcgcgagc ttctcccgga cgcgacggct
     3961 gccgggtgaa cgaagtcgtc gtcgcggacg gggtcgtcgt cgcggtggag gggcgcgggt
     4021 gacgtcgctt gggagcgagt ggtcccgcga cgggccgccg acccggaccc gtcggcgggc
     4081 ccgggcgcgt gcctggcgtc gacggctggt cgccgcgctg gccatcgtgc tcgccgtcgg
     4141 cctggtggcg gtcgcgggcg gctacgggta cgcccgcttc cgcttcgccc agatctcctc
     4201 cgtccgcctt ccggggctgg ccaggaggac ggccggcacc ggtgcgccgt tcaacgtcct
     4261 ggtggtgggc tccgacacga gagccgacct gcggcccggc gaggccgccg cctacggcag
     4321 cagcacggcc gtgaccggcc agcgcagcga cgtgatgatg gtcgtccatc tcgacccggg
     4381 caccggccgc gcgagcatcc tgtcgatccc ccgggacctg ctcgtcccga tcgcgggcac
     4441 cgggcgccgg gaccggatca acgcggcgtt cagcggcggg ccggggcggc tcgtcgcgac
     4501 gatccagggg ggcctcggca tccccatcca ccattacctg ctggtcaact tcgacggctt
     4561 ccaggcgatc gtcaatgccc tcggcgggat cgacctggac ttcccctacc ggtcccggga
     4621 cgcctactcc gggctggacg tcacccgagc cggctgtcag cacctgggcg gccatgcggc
     4681 gctggcgctg gcccgcagcc gccacttcga gtactggaag gacggttcct ggcacgtcga
     4741 cccgttgagc gacctgagcc gcatccgccg ccagcagacg ttcctccggg cggtggtggc
     4801 cagggcgcgc tcccgggggc tgacggaccc gctcgccgcc aacgcgttcg tcggcgcggt
     4861 ggtgcacgac atcaccaagg acagcgcgct gtcggtcggc gaggccgtcg ggctggtcag
     4921 ccgcttccgc tcccttgact cgaagacgct cgccagctac acgctgccga ccaccgcggc
     4981 caccggctac cccggctacg gggacgtgct gctgctgcgc caggccgccg cccgccaggt
     5041 cgtcgcgcgc ttcctcggcc gcccggcgac cgtgagccag gcgggagcag gcggccacga
     5101 ggcgagcgtc ggcggcacca cggccccgcg cccgagctcc gcgcctgctc ccgcccttcc
     5161 ggccacggcc gtaccgggct tcgacccccg cccatgctga ccggctgccc ggcagccgtg
     5221 agctcctctc cggcgcggcg cccggacggt cagcgcagcg acgccacgcc gcggacgcgg
     5281 tcgacgtccc ac
//
LOCUS       DASPYY010000267         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1030967, whole genome shotgun sequence.
ACCESSION   DASPYY010000267 DASPYY010000000
VERSION     DASPYY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030967"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VEP73_04075"
     CDS             complement(<1..362)
                     /locus_tag="VEP73_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYT25646.1"
                     /translation="MPEPQVVTAAVGRNVRAERSRKAWTLDDLAARSGVSKGMLVQVE
                     QARSNPSIATICRLATALGVSVASLVEAPEVPTARVVPPGQGVVLWNGKPGSTSTLFV
                     GSGTREQVELWDVRMAPH"
     gene            480..641
                     /locus_tag="VEP73_04080"
     CDS             480..641
                     /locus_tag="VEP73_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25647.1"
                     /translation="MGILVLLALLVLVDVLALRHGVDSRPGFEVLRPGVSSSTTGPGR
                     LTVGPGRRS"
     gene            660..1511
                     /locus_tag="VEP73_04085"
     CDS             660..1511
                     /locus_tag="VEP73_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT25648.1"
                     /translation="MSLGAPLGLGSGLLWGTGDFVGGLQSRRVAPTAVAIWSQVAGAV
                     VLAGVLAVTGHRPALAGVALGAVGGIGGGAALLLFYRGLAEGAMSVVAPVSACGAVVP
                     VVVAFAEGTPPGAAPTAGIVAAIAGVVLVSRPARAERHGGGTPPRVVAMALGAALGFG
                     CFYVFVDAGSAVPGGSPLWVVAGARGGSLVAMLAIAGLGRRSVPWPGRRLWAVALAGI
                     LDTTANLLFAYASTIGNLGVIGVLASLYPVTTVLLARFVLAERLSGPQAAGVVLALAG
                     VALLGAG"
     gene            1639..>2110
                     /locus_tag="VEP73_04090"
     CDS             1639..>2110
                     /locus_tag="VEP73_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYT25649.1"
                     /translation="MDDDLTLALELADLADAITLEHFRSDRLVVETKPDMTPVSEADR
                     AVEQLLRKRIAEERPGHGVVGEEFGAEGSRHARWILDPIDGTKNYVRGIPVFATLIAL
                     ERGGVGEVGVCSAPALGARWWAARGTGAFADGRPIRVSGVTHVGDAQLCHSSLDL"
BASE COUNT          221 a          791 c          773 g          325 t
ORIGIN      
        1 tcgtgcgggg ccatgcggac gtcccacagc tcgacctgtt cgcgcgtgcc cgaccccacg
       61 aacagggtgc tcgtgctccc cggcttcccg ttccacagca ccacgccctg gcccggcggc
      121 acgacccgcg cggtgggcac ctctggcgcc tcgaccagcg aggccaccga gaccccgagc
      181 gcggtggcca gccggcagat ggtggcgatg ctcgggttgg accgtgcctg ctcgacctgg
      241 acgagcatgc ccttgctgac ccccgaccgg gcggccaggt cgtcgagtgt ccacgccttg
      301 cggctgcgct ccgcgcggac gttgcggccg accgcggcgg tcaccacctg cggctctggc
      361 aacgcttccc ctcctcgaca acaaaatatt gttcgacagg tacaacgtag tgctattaca
      421 atggagtgta ccagacggtc agtatgatcg tccctagcca gagggaacgg aggcttctca
      481 tggggatcct ggtgctgctc gccctgctcg tcctggtcga cgtgctcgcg ctcaggcacg
      541 gcgtcgactc ccgtcccggc ttcgaggtgc tgcgccctgg tgtgtcgtcc tcgacgaccg
      601 gtccaggccg cctcacggtc ggcccggggc ggcgcagcta gccgagggcg ccggcgcccg
      661 tgtcgctcgg tgcaccgctc gggctcggct ccggcctgct gtgggggacg ggggatttcg
      721 tcggcggcct gcagtcccgc cgggtggcgc cgacggcggt cgccatctgg agccaggtcg
      781 cgggcgcggt cgtcctggcc ggcgtcctgg cggtcaccgg gcaccggccg gcgctcgcgg
      841 gggtggccct gggggccgtc ggcggcatcg gcgggggcgc ggcgctcctg ctcttctacc
      901 gcggcctggc cgagggggcg atgtcggtcg tcgcgcccgt ctccgcctgc ggggcggtcg
      961 tgcccgtcgt ggtcgccttc gccgagggaa cgccgccggg ggcggccccc accgccggca
     1021 tcgtggcggc catcgccggc gtcgtgctcg tgtcccgccc ggcgcgggcc gagcgccacg
     1081 gcggcggcac cccgccccgg gtcgtcgcca tggcgctcgg cgccgcgctc ggcttcggct
     1141 gcttctacgt cttcgtcgac gccgggtcgg ccgtgcccgg cggctcgcca ctctgggtcg
     1201 tcgccggagc ccggggcggg tccctcgtcg ccatgctggc gatcgccggg ctcgggcgcc
     1261 ggtccgtgcc gtggcccggg cgccgcctgt gggcggtcgc gctcgccggc atcctggaca
     1321 cgacggcgaa cttgctgttc gcctacgcct cgacgatcgg caacctgggc gtgatcggcg
     1381 tcctcgcctc gctctacccg gtgacaaccg tgctgctcgc ccgtttcgtc ctggccgagc
     1441 ggctgtccgg cccgcaggcc gccggggtcg tcctcgccct ggccggggtc gccctgctgg
     1501 gcgcgggctg agcgcgcgct gcgacgtagc gaactggtgt tcgccaagac cctctaacca
     1561 gcttcgggtg gcccgaaacc tctgcagacc tcttacggac caccgcacgg ggcgggccgg
     1621 gtcgggtagg ctcggccaat ggacgacgac ctgaccctcg cgctcgagct ggccgacctg
     1681 gcggacgcca tcacgctcga gcatttccgg tccgacaggc tggtggtcga gaccaaaccc
     1741 gacatgaccc cggtgtccga ggccgaccgc gccgtcgagc agctcctgcg caagcgcatc
     1801 gccgaggagc gccccggcca cggcgtggtg ggggaggagt tcggtgccga ggggagccgg
     1861 cacgcgcgct ggatcctcga tccgatcgac ggcaccaaga actacgtccg gggcatcccc
     1921 gtcttcgcca cgctgatcgc cctggagcgt ggcggcgtcg gcgaggtcgg cgtgtgctcc
     1981 gcgcccgccc tcggcgcgcg ctggtgggcg gcccgcggca cgggcgcgtt cgcggacggc
     2041 cggcccatcc gcgtctcggg cgtcacccac gtgggcgacg cgcagctctg ccactcgagc
     2101 ctcgacctgt
//
LOCUS       DASPYY010000268         7605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_469810, whole genome shotgun sequence.
ACCESSION   DASPYY010000268 DASPYY010000000
VERSION     DASPYY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469810"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2309)
                     /gene="glnD"
                     /locus_tag="VEP73_04095"
     CDS             complement(<1..2309)
                     /gene="glnD"
                     /locus_tag="VEP73_04095"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HYT25650.1"
                     /translation="MLSDVRAASALTLADGGAPARRRASAVDGALRALADDATAALAG
                     TAVAAVGGYGRGDLAPHSDIDLLVLTGSRAGIGPEALRALLYPLWDAGFQVGHAVVTP
                     REAIERATGDLDAATSLLSARLVAGEPAVFDQLASRRRRWLARNGRRLARRVLDATTA
                     RHRCAERAGWALAPDLKGDVGGLRDLHALGWLAAVAGEARVAGDGRPGGGPGRAAGDA
                     GLVAAGELLLAVREALHAQSRRKLDRLRIDLQPAVARHLGLTGEDAADRLMAAVHTAA
                     RTIEHETATQAGELVEAVLGGPRRSGTVRLLGQGVRVEDGVLVADGRPDADEPARALH
                     LLAAKASVDRPVAPATQAWLRGVFDRPPLERWSPAARDAFLALLAGPDAASACELLDH
                     VGGWAALLPEWAGVRGRAQHDPYHRYTVDGHSFATLATLSRLRASDPTVRLATEGAGD
                     LGTLYLAALLHDVGKGSGTDHSVAGERLARRAAARIGLAPDAVDEVAGLVRWHLLLVE
                     TATRRDLDDPAVVGAVAERLGDPRRVRLLYALTVADGLATGPRAWNDWKASLVRELYQ
                     RVRRALEGGPAAGPGGGSPTELRALALAVEAAEPALAGRAERLLATFPPSYPASTPLG
                     EMVDELRLLRQPPGAGEVRHRIDEDAGDGLAAVTVCALDRPGTLARTAGVLALHRASV
                     RQAWAWSTTDGLALERFLVQPAAAPRWDRLAADLEAAYAGRLAVEARLERKARDYRAA
                     IPAPPEVRVLPDASEHSTVVEVRATDALGL"
     gene            complement(2299..2637)
                     /locus_tag="VEP73_04100"
     CDS             complement(2299..2637)
                     /locus_tag="VEP73_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016694628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYT25651.1"
                     /translation="MKLVVAVIKPFKLEEVKEALEGVGIQGLSVSEVRGFGRQRGHTE
                     VYRGAEYQVEFVPKTRLEIAVDDEQVDDVVKAIVASARTGSIGDGKVWVVPAEQVVRI
                     RTGEMGVDAL"
     gene            complement(2634..3962)
                     /locus_tag="VEP73_04105"
     CDS             complement(2634..3962)
                     /locus_tag="VEP73_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HYT25652.1"
                     /translation="MNSGDTAFVLISAALVMFMTPGLALFYGGMVRAKNVLGTVMQSV
                     FALGLVSVLWVLVGYSLAFGPDKGHLIGGLDYLGFSGVGQNPNPALSATIPHLAYAAF
                     QLMFAVITPALITGAFAERMKFSGYVVFTSLWLLLIYAPLAHWVWAPGGWIRELGALD
                     FAGGTVVHMNSGAAALAAAIIIGKRKGFGTQAHVPHNLPLTILGAGILWFGWFGFNAG
                     SALAANGLAASAFIATNTAAAAGACGWALFDALKSRKSTTLGMASGAVAGLVAITPAA
                     GFVGPMASVAIGLAAGLVCAWAVGLKFRAGFDDALDVVGVHMVGGMLGALLIGIFGNA
                     AFNPAGADGLLAGGGLALLGKQAVAVLATFVFSFGGSFVLLKLVQVTVGLRVTEEEEA
                     AGLDLAQHAETGYSFSEGAGGFAPVAGHGAPAPTPPPAQPVRVPQGDSAS"
     gene            complement(4128..4259)
                     /locus_tag="VEP73_04110"
     CDS             complement(4128..4259)
                     /locus_tag="VEP73_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25653.1"
                     /translation="MVPSIVLVLLLAAGIGVRLALGATSGSPARYDDAYERAQRRPR"
     gene            4547..4628
                     /locus_tag="VEP73_04115"
     tRNA            4547..4628
                     /locus_tag="VEP73_04115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Tyr,seq:gta)
     gene            complement(4671..5186)
                     /locus_tag="VEP73_04120"
     CDS             complement(4671..5186)
                     /locus_tag="VEP73_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYT25654.1"
                     /translation="MAETLTLPPFAVAVLRAHRRQQARLRLAAAQPPTVGLRWVEPGR
                     PPRPVELDLVFRTERGTAVNPNHASRAFATLAATVGLIAHPHMLRHALASAMAAEKEP
                     ASIIAAQLRHADGGALAQRVYIHQLPQTAPRLAGLIEGVFGPAARDLRAVSAARQQEG
                     SRREPRPASPA"
     gene            5170..5850
                     /locus_tag="VEP73_04125"
     CDS             5170..5850
                     /locus_tag="VEP73_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HYT25655.1"
                     /translation="MRVSATAIRTTLRRYGLDPAPRRADGTWRAFLRLQAAGIVACDF
                     LTADTVWLRRLYVLCFIELDTRRVHLAGVTASPDGAWVTQQARNLLLLILGERGRRLR
                     FLLRNRDAKFSRSFDDVFRWESAEVLRAPVQAPNANAYAQRWVGTVRAECLDWLLIVG
                     RAHLERVLRVYVQHDNAHRPHRALGLQPPDPPPVARSILGEDDRGAVHRRDLLGGLLH
                     DHEYRRAA"
     gene            complement(5857..6159)
                     /locus_tag="VEP73_04130"
     CDS             complement(5857..6159)
                     /locus_tag="VEP73_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25656.1"
                     /translation="MLPRLAYLTLCRSIQLLALLARGDPAKDLELLVLRHQLTVLRRQ
                     VPWPRLEPADRALLAAVSRALPRARWSCFLVQPETLLRWHRRLIAGAWTYPHLKGA"
     gene            6465..7145
                     /locus_tag="VEP73_04135"
     CDS             6465..7145
                     /locus_tag="VEP73_04135"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYT25657.1"
                     /translation="MTDLLERQLYTVGEAARLLRLPSSRLRRWLEGFTASGRYYEPVI
                     RPQPTGSDVVTWAEFVEAGFLREYRAKRVSLQHLRPVIDQMRKQFDVRYPLAHFKPYV
                     DTASRQLVLGIQRAAQLEDELALLVRLPGGQLQWAAAVQAFIDKVEFDAEGIVERMRP
                     LGKTSPVVIDPEVTFGVPQVKGIRTEVIAESYAEDGSWERVARDWHLGVEDVQAALQW
                     ELTSRKAA"
     gene            7394..7552
                     /locus_tag="VEP73_04140"
     CDS             7394..7552
                     /locus_tag="VEP73_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25658.1"
                     /translation="MLTGAGNYSRWRTLDLLVRRWPDIEDAAGSEAPPYIYAVTQQGL
                     RRVELGSN"
BASE COUNT         1059 a         2716 c         2860 g          970 t
ORIGIN      
        1 agccccagcg cgtcggtcgc acgcacctcg accacggtcg agtgctcgga ggcgtccggg
       61 agcacccgca cctccggagg tgcggggatg gcggcgcggt agtcgcgcgc cttgcgctcg
      121 agcctggcct cgacggcgag ccggccggcg taggcggcct cgaggtcggc ggcgagccgg
      181 tcccagcgcg gcgccgccgc cggctgcacc aggaagcgct cgagcgcgag cccgtccgtg
      241 gtcgaccatg cccacgcctg cctgaccgag gcgcggtgga gggccagcac accggccgtg
      301 cgcgcgagcg tgccggggcg gtcgagggcg cagacggtga ccgccgcgag accgtccccg
      361 gcgtcctcgt cgatccggtg gcgcacctcg cccgcgccag ggggctggcg cagcaggcgc
      421 agctcgtcga ccatctcgcc gagcggggtc gatgccgggt aggacggggg gaacgtggcc
      481 agcagccgct cggcccgtcc ggccagggcc ggctcggccg cctcgaccgc cagggccagg
      541 gcccggagct cggtgggcga gccgccgcca gggccggccg ccggcccgcc ctcgagcgcg
      601 cgccgaaccc gctggtacag ctcgcggacg agcgacgcct tccagtcgtt ccacgcccgc
      661 ggcccggtgg cgagcccgtc ggccacggtg agggcgtaga gcagccgcac gcgccgcggg
      721 tcgccgagcc gctcggcgac cgcgccgacc accgccgggt cgtccaggtc acgcctggtg
      781 gccgtctcca ccagcagcag gtgccatctg accaggccgg cgacctcgtc gacggcgtcc
      841 ggcgcgagcc cgatcctggc tgcggcgcgg cgggccaggc gctccccggc cacgctgtgg
      901 tcggttccgg agcccttgcc gacgtcgtgg aggagggcgg ccaggtacag ggtgccgagg
      961 tcgccggccc cctcggtggc gagccgcacg gtcgggtcgg aagcccggag ccgtgagagc
     1021 gtggccaggg tcgcgaacga gtgcccgtcc accgtgtagc ggtggtaggg gtcgtgctgc
     1081 gcccgcccgc ggacaccggc ccactcgggc agcaaggcgg cccagccccc gacgtggtcg
     1141 agcagctcgc acgctgacgc ggcgtcgggc ccggccagca gcgcgaggaa ggcgtcgcgg
     1201 gccgccgggc tccagcgctc cagcggcggc cggtcgaaca ctccccgcag ccaggcttgg
     1261 gtggccgggg ccaccggccg gtcgacggac gccttcgcgg ccagcaggtg cagcgcccgg
     1321 gccggctcgt cggcgtccgg ccgcccgtcg gcgacgagca cgccgtcctc gacccgcacg
     1381 ccctggccga gcagccggac cgtgcctgat cggcggggac cgccgaggac cgcctcgacc
     1441 agctccccgg cctgggtggc cgtctcgtgc tcgatggtcc tggcagcggt gtggaccgcg
     1501 gccatgagcc ggtcggccgc gtcctccccg gtgaggccca ggtggcgggc gacggcgggc
     1561 tgcaggtcga tccggagccg gtcgagcttg cgcctcgact gggcgtggag ggcctcccgg
     1621 accgccagca gcagctcgcc ggctgccacc agccccgcgt cgccggccgc ccggccgggc
     1681 ccgccgcccg gtcggccgtc gcccgccact cgggcctcac cggcgacggc ggccagccag
     1741 ccgagcgcgt gcaggtcacg gaggccgccg acgtcgccct tcaggtccgg agcgagtgcc
     1801 cagccggccc gctccgcgca gcggtggcgg gcagtcgtgg cgtcgagcac gcgacgcgcc
     1861 agccgccgcc cgttccgtgc gagccagcgc ctccggcggc tcgccagctg gtcgaagacg
     1921 gccggctccc cggccaccag ccgggccgag agcagcgagg tcgccgcgtc gaggtcaccc
     1981 gtggcacgct cgatcgcctc gcgtggcgtc accaccgcgt gccccacctg gaacccggcg
     2041 tcccagagcg ggtagagcag cgcccgcagc gcctccgggc cgatccccgc ccgggagccg
     2101 gtgaggacca gcaggtcgat gtcggagtgg ggagcgaggt cgccccggcc gtagccgccg
     2161 acggcggcta cggcggtgcc ggcgagagcc gccgtagcgt cgtcggcgag tgcccggagc
     2221 gccccgtcca cggccgaggc gcgccgacgg gccggcgcgc ctccatcagc cagggtcagg
     2281 gcgctggcgg cgcggacgtc agagagcatc cacccccatc tcgccggtgc ggatccgcac
     2341 gacctgttcg gcgggcacca cccagacctt gccgtcgccg atcgacccgg tccgggcgct
     2401 cgccacgatc gccttgacga cgtcgtcgac ctgctcgtcg tcgaccgcga tctcgagccg
     2461 ggtcttcggg acgaactcca cctggtactc ggcgccgcgg tagacctcgg tgtggccgcg
     2521 ctgccggccg aagccgcgga cctccgagac gctcagcccc tggataccga caccctcgag
     2581 ggcctccttc acctcttcga gcttgaacgg cttgatgacc gcgacgacca gcttcatgag
     2641 gccgagtctc cttgggggac gcgcaccggc tgggccggcg ggggagtggg cgcgggcgcg
     2701 ccgtgcccgg cgaccggggc gaacccgccg gcgccctccg agaacgagta gccggtctcg
     2761 gcgtgctggg cgaggtccag gccggccgcc tcctcctcct cggtcacgcg caggccgacc
     2821 gtcacctgca cgagcttgag gaggacgaag ctcccgccga acgagaagac gaacgtggcg
     2881 aggaccgcga ccgcctgctt gccgaggagg gccaggccgc caccggcgag caggccgtcc
     2941 gctcctgcgg ggttgaacgc cgcgttgccg aagatcccga tgagcagggc gccgagcatg
     3001 ccgccgacca tgtgcacgcc gaccacgtcg agcgcgtcgt cgaagccggc ccggaacttc
     3061 aggcccaccg cccaggcgca gacgagcccg gctgcaaggc cgatcgcgac cgacgccatc
     3121 gggccgacga agccggcggc cggggtgatc gcgacgagcc cggcgaccgc gcccgaggcc
     3181 atcccgagcg tggtcgactt gcggctcttc agcgcgtcga acagggccca gccgcaggcg
     3241 cccgcggcgg ccgcggtgtt ggtggcgatg aacgcggagg cggcgagtcc gttggcggcc
     3301 agcgcgctgc cagcgttgaa gccgaaccac ccgaaccaca ggatgccggc gccgaggatg
     3361 gtgagcggca ggttgtgggg gacgtgggcc tgggtgccga accccttgcg cttgccgatg
     3421 atgatcgccg ccgcgagcgc cgccgcgccg gagttcatgt gcacgaccgt gccgccggcg
     3481 aagtccagcg cgccgagctc gcggatccag ccgcccggcg cccacaccca gtgggcgagc
     3541 ggcgcgtaga tgaggagcag ccagaggctg gtgaagacga cgtagccgga gaacttcatg
     3601 cgctcggcga acgccccggt gatgagcgcc ggggtgatga ccgcgaacat cagctggaac
     3661 gcggcgtagg cgagatgcgg gatggtggca gacagggcgg ggttggggtt ctgcccgacg
     3721 cccgagaagc cgaggtagtc gagcccgccg atgaggtggc ccttgtccgg cccgaatgcg
     3781 agcgagtacc cgaccagcac ccagaggacc gagacgagcc cgagcgcgaa cacgctctgc
     3841 atcaccgtgc cgagcacgtt cttggcccgc accatgcccc cgtagaagag cgccagcccg
     3901 ggggtcatga acatgaccag cgccgccgag atcaggacaa atgcggtgtc cccggagttc
     3961 acagagcctc ccttgctcgc tgtccgtgcg agagatgggt acctgaccga tgtttcggcc
     4021 gtgttacccg ggagttgatc cgtcgttaat ctcggacgtc ccgtgggctc gtcgacggcc
     4081 ctgccgctga cggccgggcc ggggcccgca ccgggctcag gggacggcta ccgggggcgg
     4141 cgctgggcgc gctcgtaggc gtcgtcgtac cgcgcgggcg agcccgacgt ggcgccgagc
     4201 gccagcctga ccccgatgcc ggctgccagc aggagcacga ggacgatgct cgggaccatg
     4261 gtcatgcctc ctgggcggcg cgcccgcggc cgacggccgg cctcgcgcgt ccgcggtgcc
     4321 gggcgccgag ctgtcacggg gaagacggac agcatgttcg cagcaacacc gagcggaacg
     4381 gatccgcagg attcggcaga aacccagtaa agatctgcac accgagcatg gccaccgagt
     4441 gccctgagcc gggcgaacgc ggttgtgaga ggcggattcc gccggtatca tccgccctcg
     4501 gtccgcgaga tcgccggggt cgcccgaggc ctcgccggct gaccctggca gggtgcccga
     4561 gcggtcaaag ggagcggtct gtaaaatcgc cggcaatgcc ttcacaggtt cgaatcctgt
     4621 ccctgccacg ctacctctga cgtgcgaaaa cgcggtcgcc gctcggccgg tcacgcgggg
     4681 ctcgccggtc ggggctccct ccggcttccc tcctgctgac gcgcagccga caccgcccgc
     4741 aggtcccgcg ccgcggggcc gaatacgccc tcgatcagcc cggccagccg gggtgcggtc
     4801 tgtgggagct ggtggatgta gacgcgttgg gcgagcgcgc cgccgtcggc gtggcgcagc
     4861 tgcgcggcga tgatgctggc cggctccttc tcggccgcca tcgccgacgc cagcgcgtgc
     4921 cgcagcatgt ggggatgggc gatgagcccg acggtggcgg cgagggtggc gaaggcgcgg
     4981 ctggcgtggt tggggttgac cgccgtgccc cgctcggtgc gaaacaccag gtcaagctcg
     5041 accggccggg gcggccggcc gggctcgacc cagcgcagcc cgaccgtggg gggctgggca
     5101 gcggcgaggc gcagccgggc ctgctggcgg cggtgggcgc gcagcaccgc cacggcgaac
     5161 ggtggcagcg tgagggtctc ggccaccgcg atccgcacga cgctgcgccg ctatgggctc
     5221 gacccggcgc cacggcgagc cgatggcacc tggcgggcgt tcctgcgcct gcaggccgcc
     5281 gggatcgtgg cgtgcgactt cctcaccgcg gacacggtct ggctgcggcg gctgtatgtg
     5341 ctgtgcttca tcgagctgga cacccgccgg gtccatctgg ccggcgtgac cgccagcccc
     5401 gacggcgcct gggtcaccca gcaagcccgc aacctcctgc tgctgatcct gggcgagcgg
     5461 ggacgtcggc tgcgcttcct gctccgcaac cgcgacgcga agttctcccg cagcttcgac
     5521 gacgtgttcc gctgggagag tgccgaggtg ctgcgggcac ccgtgcaggc acccaatgcg
     5581 aacgcctacg cgcagcggtg ggttggcacc gtccgcgccg agtgcctgga ctggctgctg
     5641 atcgtcggac gcgcccacct cgagcgggtc cttcgggtct atgtccagca tgacaacgcc
     5701 caccgtcccc accgggcgct gggcctgcaa ccaccagacc cgccgcccgt cgcgcggagc
     5761 atcctcggcg aggatgaccg aggcgctgtg catcgacgcg acctgctcgg cggtctcctc
     5821 cacgaccacg agtaccggcg agctgcatga acgcgtttac gcacccttca ggtgcgggta
     5881 ggtccacgcg ccggcgatca gccggcggtg ccagcgcagc aacgtctccg gctgcacgag
     5941 gaagcaggac cagcgggctc gggggagcgc gcggctgacg gcggcgagca gggcgcggtc
     6001 ggcaggctcg agcctcggcc atggaacctg gcggcggagc acggtgagct ggtggcgcag
     6061 gacgaggagc tccaggtcct tggccggatc gccgcgggcg agcagggcga gcagctggat
     6121 ggaacggcac agcgtcaggt aggcgagcct tggcagcatg cggggtagcc tcccggtcag
     6181 ggcagcaggg tgccaccata ccgagccaca ggcctgcgac tccgcagctc aaaggccacg
     6241 tccgactttg cgcacccttc aggcacggcg tcggcggtag cgcgtggggt gtgggtatcc
     6301 attctgcagc tcacaggcaa catccgacgt cccgacccta ccggctggtc gagccgcggg
     6361 gtaacccggg accgccgtcg ccggcgcttg tcgggggttg atgcgatcat ctgtgcgcga
     6421 cctgccgctt cacggtaggg ttcgcggtcg gggagggtgc cgacatgacc gacctgcttg
     6481 agcggcagct ctacacggta ggcgaggccg cgcggctgct caggctgccg agcagcaggc
     6541 tgcggcggtg gctcgagggc ttcaccgcca gcgggcgcta ctacgagccg gtgatccgcc
     6601 cgcagccgac cggcagcgac gtggtgactt gggcggagtt cgtcgaggcc gggttcctcc
     6661 gggagtaccg ggccaagcgt gtctcgctgc agcatctgcg gccggtcatc gaccagatgc
     6721 gaaagcagtt cgacgttcgg tatccgctgg cgcacttcaa gccgtacgtc gataccgcca
     6781 gccggcagct ggtgcttggc atccagcggg ccgcccagct cgaggatgag ctggcgctgc
     6841 ttgttcgcct gccaggtggg cagctgcagt gggccgcggc ggtgcaggcg ttcatcgaca
     6901 aggtcgagtt cgacgccgag gggatcgtcg agcgaatgcg cccgcttggg aagacctccc
     6961 ctgtcgtcat cgacccagag gtgaccttcg gcgtcccgca ggtcaagggc atccggaccg
     7021 aggtcatcgc cgagagctac gccgaggacg gttcatggga gcgcgtcgcg cgtgactggc
     7081 acctgggcgt cgaggatgtg caggcggcgc tgcagtggga gttgacaagc cgaaaggcgg
     7141 cctagcgccg ccgtggtctt ctacctggac gcgagcatcc ccttcccggt cagccaggcg
     7201 ttcgcgcttg tccgcgacga tgtgttgtat ccgggagggc ccggttgtcc catcacgaca
     7261 ccggacgcga aggaccacga gtggctccca gtcgccgggc agcacggatg ggtggtgctc
     7321 atgcgcgaca aagcacgtgc ggtcgcggcc cggagaacgc cgggcgctac gcgagaacgg
     7381 tgtccgtgcg ttcgtgctca ccggcgctgg gaactactcg aggtggcgca cgctcgacct
     7441 gctggtgcgg cgctggcctg acatcgaaga tgccgcgggg agcgaggccc ctccctacat
     7501 ctacgcggta acgcagcagg ggctccgacg ggtcgagctg ggctcgaact gagcagcggc
     7561 ggctcggtcg agtggccggg gagttgcgcc cctggctccc acaga
//
LOCUS       DASPYY010000269         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2716275, whole genome shotgun sequence.
ACCESSION   DASPYY010000269 DASPYY010000000
VERSION     DASPYY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716275"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="VEP73_04145"
     CDS             <1..1812
                     /locus_tag="VEP73_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HYT25659.1"
                     /translation="AGALAFAPADRPVGAHLYADRREAYAELEEHGYYQRPPARPEGR
                     WAVYRFNAVTPRVAFAAAPGADAGAAVAALGRLAGGAGQVLAGSADAAAERSRAAAIR
                     DVVAWNTVWDPVNARPYTASTRAWVAPKFGGWAVWQLDAFFHVLLAARAGDRATALAN
                     LHAALAGATAQGNLAALRSGRTDWVDRSHPPVGALAVWCLHRATGDRRALAESWPVLA
                     RAFRWWFAARDGNGNGLLEYGSSPVGDGHFVHTKLAAMDESAMDNSPVHDEAVFDPAT
                     HTLDVEDVGLNSLLVLEGDLLAALAVEAGRAGEAPALAAASAALAGRVRSALWDPARG
                     IYASRRWDGRFARGLAPTSFYPMLAGIATPEQAATMVREHLQDPRRFGGPWPVASTPH
                     DDPASLDNVYWRGRVWPCLNYLVYRALRRYRFDAAAAELAARGVAMFERGWAERRSFE
                     NYNQRTGEGGDSVDADPFYTWGALLPLIGEMELADLDPWDGLVVGCEGTAPGRAELPA
                     PSGRWRVELAPAETRLFHDGELVLEAGFRGRLRDVRLDPDEAALTVPPLPHGGALRAR
                     VRGGLAEAVLPPDPAARRLRLRRAECPARLEVRYEPG"
     gene            complement(1972..>3013)
                     /gene="menC"
                     /locus_tag="VEP73_04150"
     CDS             complement(1972..>3013)
                     /gene="menC"
                     /locus_tag="VEP73_04150"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HYT25660.1"
                     /translation="TETERDVLLVRVRADAGDGWGECVAGAEPLYSSEYVDGAQDVIG
                     RHLLPRLFALPDARAHLVGPALAAVRGHPMAKAALEMAVLDAELRVAGVPLGAHLGAV
                     RDAVDCGVSVGIMGSVAELLDAVAGYVAAGYRRVKLKIEPGWDLEPVRAVRERFGSDL
                     ALQVDANAAYTLADARRLAGLDAFDLLLLEQPLAEDAVRAHAALARMVRTPICLDESI
                     TSARAAADAIALGACAVVNVKAGRVGGYLEARRVHDVCAAHGVPVWCGGMLETGLGRA
                     ANVALAALPNFTLPGDTSASDRYYHADLTPPFVLEDGRLRVPAGPGLGVDPVPERLRE
                     LTTSVRELAGDA"
BASE COUNT          339 a         1206 c         1128 g          340 t
ORIGIN      
        1 gccggcgcgc tcgccttcgc gcccgccgac cggccggtcg gcgcccacct ctacgccgac
       61 cggcgcgagg cgtacgccga gctcgaggag cacggctact accagcggcc gcccgcccgc
      121 ccagagggac gctgggccgt gtaccggttc aacgcggtca ccccgcgggt cgcgttcgcc
      181 gccgcgccgg gcgcggacgc gggcgcggcc gtggccgcgc tcggccggct cgcgggcggg
      241 gcagggcagg tcctcgccgg gagcgccgac gcggccgcgg agcgctcccg ggcggcggcc
      301 atccgcgacg tggtcgcctg gaacaccgtc tgggacccgg tcaacgcccg gccctacacg
      361 gcgtcgaccc gcgcctgggt ggctccgaag ttcggcggct gggccgtgtg gcagctcgac
      421 gccttcttcc acgtgctgct ggccgcccgg gccggcgacc gcgcgaccgc gctcgcgaac
      481 ctgcacgccg cgctcgccgg cgccaccgcg cagggcaacc tcgccgccct gcgcagcggc
      541 cgcaccgact gggtcgaccg ctcgcacccg ccggtcggcg cgctggccgt ctggtgcctc
      601 caccgggcga cgggcgaccg gcgggcgctc gccgagagct ggccggtcct cgcccgggcc
      661 ttccgctggt ggttcgctgc cagggacggc aacggcaacg gcctgctcga gtacggctcc
      721 tccccggtcg gcgacggcca cttcgtgcac accaagctcg cggccatgga cgagtcggcg
      781 atggacaact cccccgtcca cgacgaggcg gtcttcgacc cggcgacgca caccctcgac
      841 gtggaggacg tcggcctgaa cagcctgctc gtgctcgagg gcgacctgct cgcggcgctg
      901 gccgtcgagg ccggccgggc cggcgaggca ccggcgctgg ccgcggcgag cgccgcgctc
      961 gccgggcggg tccggtcggc cctgtgggat cccgcgcgcg ggatctacgc cagccgccgg
     1021 tgggacggcc ggttcgcccg cggcctcgcg ccgaccagct tctacccgat gctcgccggc
     1081 atcgcgaccc cggagcaggc cgcgacgatg gtgcgcgagc acctgcagga cccccgccgc
     1141 ttcggcggtc cctggccggt cgcgtccacg ccgcacgacg acccggcgtc gctcgacaac
     1201 gtctactggc gcggccgcgt ctggccctgc ctgaactacc tcgtgtaccg cgcgctgcgc
     1261 cgctaccgct tcgacgcggc cgccgccgag ctggcggcgc gcggcgtcgc gatgttcgag
     1321 cgaggctggg ccgagcggcg ctcgttcgag aactacaacc agcggaccgg cgagggcggc
     1381 gactcggtcg acgccgaccc gttctacacc tggggggcgc tcctgccgct catcggcgag
     1441 atggagctcg ccgacctcga cccgtgggac gggctggtgg tcggctgcga gggcacggcg
     1501 ccgggccgcg ccgagctgcc cgctccgtca gggcgctggc gggtcgagct cgcgccggcc
     1561 gagacccgcc tcttccacga cggcgagctg gtcctcgagg ccggcttccg gggccggctc
     1621 cgggacgtcc gcctcgaccc cgacgaggcc gccctgaccg tgccgcccct cccccacggc
     1681 ggcgccctcc gggccagggt gcgcggcggg ctggccgagg ccgtcctccc gcccgaccct
     1741 gccgcccgcc gcctgcgcct gcgccgcgcc gagtgtccgg cccggctcga ggtgcggtac
     1801 gagccgggct gacgtgcccg cccgcctcag gccgggtgcc gcatccacca cgccgacggt
     1861 caccccgtcg gtcgaggtgg acgagcagca gcacccgggg cggcccgccg aagcgccacc
     1921 gcaggtgggg gcggccgtcg cgctcgacgc gctcaggcat cgccggccag ctcaggcatc
     1981 gccggccagc tcccgcaccg aggtggtgag ctcccggagg cgctcgggga cggggtcgac
     2041 gccgaggccc ggcccggccg gcacgcgcag gcggccgtcc tccagcacga acggtggcgt
     2101 caggtcggcg tggtagtagc ggtcggaggc ggaggtgtcg cccggcaggg tgaagttggg
     2161 cagcgcggcc agggcgacgt tcgccgcccg cccgaggccc gtctcgagca tcccgccgca
     2221 ccagaccggg acgccgtgcg cggcgcagac gtcgtgtacc cggcgggcct cgaggtagcc
     2281 gccgacccgc ccggccttga cgttgacgac cgcgcacgcc ccgagcgcga tcgcgtccgc
     2341 cgccgcccgc gccgaggtta tcgactcgtc caggcagatc ggggtccgca ccatcctggc
     2401 cagcgccgcg tgcgcccgca cggcgtcctc ggccagcggc tgctcgagga gcagcaggtc
     2461 gaaggcgtcg aggccggcga gccgtctggc gtcggcgagg gtgtaggcgg cgttcgcgtc
     2521 gacctgcagc gcgaggtcgg agccgaagcg ctcccgtacc gccctgaccg gctccaggtc
     2581 ccagcccggc tcgatcttca gcttgacgcg gcggtagccg gccgcgacgt agccggcgac
     2641 cgcgtcgagc agctcggcga ccgaccccat gatgccgacc gagacgccgc agtcgaccgc
     2701 gtcgcggacc gcaccgaggt gggcgccgag cggcacgccg gccacgcgca gctcggcgtc
     2761 gagcaccgcc atctcgagcg ccgccttggc catcgggtgg cccctgaccg cggccagcgc
     2821 cggcccgacc aggtgcgcac gcgcgtcggg cagggcgaac aggcgcggca gcaggtgccg
     2881 gccgatgacg tcctgcgcgc cgtccacgta ctcggacgag tagagcggct cggccccggc
     2941 gacgcactcg ccccacccgt cgcccgcgtc ggcccgcacc ctgacgagca gcacgtcgcg
     3001 ttcggtctcg gtc
//
LOCUS       DASPYY010000270         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_749650, whole genome shotgun sequence.
ACCESSION   DASPYY010000270 DASPYY010000000
VERSION     DASPYY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749650"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VEP73_04155"
     CDS             <1..350
                     /locus_tag="VEP73_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HYT25661.1"
                     /translation="TDQANLDKLLGELNGVPPERRAARFRCAAVLVDPRRGAWHAEAA
                     WEGRLLDAPRGANGFGYDPVFVPDGWTLTSAEAGPAAKDAASHRGRAFRALRPSIERW
                     AEAVRSEVPPARP"
     gene            579..1523
                     /locus_tag="VEP73_04160"
     CDS             579..1523
                     /locus_tag="VEP73_04160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYT25662.1"
                     /translation="MTTSAARHGDAPGRGGAHRADEPAVRLVASDLDGTLLRPDATVS
                     ERTRAAIARTLAAGVEFVMVTGRPPRGVREIAARAGVGGLAICANGGLVYDLDAGAVV
                     ASTPLGTEAGRRLVRELRAAVPGTVFASETATGFLRGTGWLRPRVPEPDGAVPDDSSV
                     DDPLALVAEPVVKLLVRNPELPFAELAARVRAAAGDDAVVTWSGPELLEVSAAGVTKA
                     FALEWLCGRLGVEAREVVAIGDMQNDLTMLAWAGRSVAVANAAEEVLEAVDEVTAANV
                     EDGVALVLERLVAEVSPARRARPASGAGRRGAGAAPRR"
     gene            complement(1450..1917)
                     /locus_tag="VEP73_04165"
     CDS             complement(1450..1917)
                     /locus_tag="VEP73_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT25663.1"
                     /translation="MSEREQPDTEALPGVLAREVWELMIDLSTSLLRQRFSRTIAELK
                     LSPPQAHALRELEPGQPLPMRDLACSLACDASNVTGIADRLEKRGLVERQVSARDRRV
                     KTLVVTPEGVATRQRLLALLYEVPSPIATLSAEEQHLLRDALRRTLAEPGERG"
     gene            complement(2052..>2923)
                     /locus_tag="VEP73_04170"
     CDS             complement(2052..>2923)
                     /locus_tag="VEP73_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25664.1"
                     /translation="VLCSASLTLGSLRRLGPPIQAPPRAARLLEAVRVSAGTGLVLSG
                     LTWLTGRLPAVGVAFAAAGVAVGLRPLLRFLPPGTLRARPGLPVAVLSRGLLTFAFFG
                     ADTYVPLTITAVRGQSTTVGSLAVTASTLSWTAASWVQERRARVSSGRSLVATGLAIL
                     VAGIACIAAATHPAVPLAVGIAGWGIAGFGIGLAYSPISLLVLKEAAPGQEGSATAAM
                     QLADNLGVALGAGLGGVAVALGAAAGWRPGAGVAIVFAMTACVGAVGVAVALRLPAGV
                     LAAPAPDPVPPRG"
BASE COUNT          361 a         1098 c         1157 g          307 t
ORIGIN      
        1 cgaccgacca ggccaacctc gacaagctcc tcggcgagct gaacggggtg ccgcccgagc
       61 ggcgcgccgc ccggttccgt tgcgcggcgg tgctggtcga cccgcgacgg ggcgcctggc
      121 acgcggaggc ggcctgggag ggccgcctgc tcgacgcgcc gcggggcgcg aacggcttcg
      181 gctacgaccc cgtgttcgtg cccgacggct ggacgctgac gagcgccgag gccggcccgg
      241 ccgccaagga cgccgcctcc caccgcggaa gggcgttccg cgcgctgcgc ccgtccatcg
      301 agcggtgggc ggaggccgtc cgctccgagg tcccgcccgc gcgcccgtaa cgatctcgga
      361 acggtggcag gggatccttc ccacctgggg ggcacacagg ggggcgcacg gggggacgcg
      421 ggcgccgccc gggaccggcc ggtcccgggc ggcgcctcgg cgcgcccgtc agccgcccgc
      481 gggcgaggaa tgcgcgcacg aaccgcgcgg tatcccatac ggggtcgact acggggtcga
      541 cccgggccac gggttctccg gcccgctacc gtgggggcat gacaacctcc gcggcacgcc
      601 acggggacgc gcccgggcgc ggcggcgcgc accgcgcgga tgagccggcg gtccggctcg
      661 tcgcgagcga cctcgacggc accctcctcc ggccggacgc gaccgtgtcg gagcggacca
      721 gggccgccat cgcgcggacc ctcgcggccg gcgtcgagtt cgtgatggtc accggccggc
      781 cgccccgcgg cgtgcgcgag atcgcggccc gggcgggcgt gggcgggctc gcgatctgcg
      841 cgaacggcgg gctcgtctac gacctcgacg ccggcgcggt ggtcgcgtcc accccgctcg
      901 ggaccgaggc cggccggcgg ctcgtccgcg agctgcgcgc cgccgtgccc gggaccgtgt
      961 tcgcctccga gaccgccacc gggttcctgc gcgggaccgg ctggctccgg ccgcgggtcc
     1021 cggagcccga cggcgccgtg cccgacgaca gctccgtcga cgacccgctc gccctggtcg
     1081 ccgagccggt cgtcaagctg ctggtgcgca atcccgagct gccgttcgcc gagctggccg
     1141 cgcgggtccg cgcggctgcc ggggacgacg cggtcgtgac ctggtccggc cccgagctgc
     1201 tcgaggtgtc ggccgccggc gtgaccaagg cgttcgcgct cgagtggctg tgcgggcggc
     1261 tcggggtgga ggcccgggag gtggtcgcga tcggcgacat gcagaacgac ctcaccatgc
     1321 tcgcctgggc cgggcgttcg gtggccgtgg ccaacgccgc ggaggaggtg ctcgaggcgg
     1381 tcgacgaggt caccgccgcc aacgtcgagg acggcgtcgc cctggtcctc gagcgcctgg
     1441 tcgcggaggt cagccccgct cgccgggctc ggccagcgtc cggcgcaggg cgtcgcggag
     1501 caggtgctgc tcctcggcgc tgagcgtggc gatcggggac gggacctcgt agaggagcgc
     1561 gagcaggcgc tgccgcgtcg ccaccccctc gggcgtcacg acgagggtct tgaccctccg
     1621 gtccctcgcc gacacctgcc gctcgaccag cccgcgcttc tcgaggcggt cggcgatgcc
     1681 cgtcacgttc gacgcgtcgc aggccaggct gcacgccagg tcccgcatgg ggagcggctg
     1741 gccgggctcg agctcgcgca gcgcgtgggc ctggggcggg gacagcttga gctcggcgat
     1801 ggtgcggctg aagcgctggc gcagcaggga ggtcgacaga tcgatcatga gctcccagac
     1861 ctcccgagcc agcacgcccg ggagggcctc ggtgtccggc tgctccctct cgctcatccc
     1921 aggaagcata gcatccgaat agttgacgac ctcagccatt ctcgctccgg ccccaggtag
     1981 ctcctcgatc gagcggctcc tcgatcgggc gcgcctgact cctggcccag gggcgggcac
     2041 cgcccgcgct cctacccgcg gggcggcacc gggtcgggtg ccggcgcggc gaggacaccc
     2101 gccggcaggc gcagggcgac ggccacgccg acggccccca cgcatgccgt catcgcgaag
     2161 acgatcgcga cgccggcgcc cggtcgccac ccggcggcgg cgccgagcgc gacggccacc
     2221 ccgccgagcc ccgcgccgag cgcgacgccg aggttgtcgg cgagctgcat cgcggccgtg
     2281 gccgacccct cctgcccggg cgccgcctcc ttcagcacga gcagcgagat gggggagtac
     2341 gccaggccga tgccgaagcc ggcgatgccc cagccggcga tccccaccgc caggggcacc
     2401 gccggatggg tggcggcggc gatgcaggcg atcccggcca cgaggatggc gaggcccgtg
     2461 gcgacgaggc tgcggccgct cgagacccgg gccctgcgct cctgcaccca cgaggcggcg
     2521 gtccaggaga gggtcgaggc ggtcaccgcc aggctcccga cggtcgtgct ctggccccgg
     2581 acggcggtga tggtgagcgg cacgtaggtg tcggcgccga agaacgcgaa ggtgagcagg
     2641 ccacgggaga gcaccgcgac ggggagcccc ggacgggcgc gcagcgtccc gggcggcagg
     2701 aagcggagga gcgggcgcag cccgacggcc acacccgccg cggcgaacgc gacgccgacg
     2761 gccggcagcc ggcccgtgag ccaggtgagc ccggacagca ccaggccggt gccggccgag
     2821 acccggaccg cctcgagcag gcgggcggcc cgcgggggcg cctggatcgg cgggccgagg
     2881 cggcggagcg agccgagggt gagcgacgcc gagcagagga cca
//
LOCUS       DASPYY010000271         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1453409, whole genome shotgun sequence.
ACCESSION   DASPYY010000271 DASPYY010000000
VERSION     DASPYY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453409"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VEP73_04175"
     CDS             complement(<1..1479)
                     /locus_tag="VEP73_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HYT25665.1"
                     /translation="MKPDDWFLRADERGNPATEIDRRRGDGRAWTEGNLAEPLVHGAA
                     YYRRLLAVLRSLQPGELVCVTDWRGDPDERLDGPGTELGTVLAELVARGVEVRGLLWR
                     SHPSRTHFNEEENLRLGELVNRAGGEVLLDERVRGPGSQHQKLVLVRHPGREDDDVAF
                     LGGIDLAHGRRDDERHLGDPQAIQMDERYGPRPPWHDVQLEVRGPAVGDLDVTFRERW
                     QDPTPLDHRNPWRARLTKRVRQPRHPGPLPPMPRDPGPAGHHAVQVLRTYPAKQPPLP
                     FAPKGERSIARAYAKAFWRARRLIYLEDQYLWSVEVARLLADALCRSPDLRLVAVVPR
                     YPDQDGRLSGPPNRIGQQTALDLVREAGGDRVAIFDLENEEGTPIYVHAKVCVIDDVW
                     AAVGSDNLNRRSWTHDAELAAAVLDEARDPREPADPAGLGDGARVFARDLRLRLWREH
                     LGREPGDDADLLDPERGVEAWRHVAEALAAWHRGGRRGPRPPG"
     gene            1740..>1974
                     /locus_tag="VEP73_04180"
     CDS             1740..>1974
                     /locus_tag="VEP73_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT25666.1"
                     /translation="MTEVRPEAGIARSAALFERARARIPGGVNSPVRAFGAVGGTPRF
                     FARGQGAWLWDVDGNRYVDLVLSWGPLIAGHAHP"
BASE COUNT          216 a          741 c          755 g          262 t
ORIGIN      
        1 cccgggcggg cgcgggcccc gccggccgcc gcggtgccag gccgcgaggg cctcggccac
       61 gtgccgccac gcctcgacgc cacgctccgg gtcgagcagg tcggcgtcgt cgcccggctc
      121 gcggccgagg tgctcgcgcc acagccgcag gcgcagatcc cgcgcgaaca cccgggcacc
      181 gtcgccaagg cccgccgggt cggcgggctc cctcgggtcg cgggcctcgt cgagcacggc
      241 cgcggccagc tcggcgtcgt gggtccacga gcggcggttc aggttgtcgg agcccacggc
      301 cgcccagacg tcgtcgatca cgcagacctt cgcgtgcacg tagatgggcg tgccctcctc
      361 gttctcgagg tcgaagatgg cgacgcggtc gccgccggcc tcgcgcacga ggtccagcgc
      421 cgtctgctgg ccgatccggt tgggcggccc ggagagccgg ccgtcctggt ccgggtagcg
      481 aggcacgacg gcgacgagcc gcaggtcggg ggagcggcac agcgcgtcgg cgagcaggcg
      541 cgccacctcc accgaccaga ggtactggtc ctcgaggtag atgaggcgcc tggcccgcca
      601 gaacgccttc gcgtacgcgc gggcgatgct gcgctcgccc ttcggcgcga agggcagcgg
      661 aggctgcttg gccgggtagg tgcgcagcac ctggacggcg tggtggccgg ccggcccggg
      721 gtcgcgcggc atcggcggca gggggccggg gtgcctcggc tgccggaccc gcttcgtcag
      781 ccgggcccgc caggggttgc ggtggtcgag cggggtcggg tcctgccacc gctcccggaa
      841 ggtgacgtcc aggtcgccca ctgccggccc cctgacctcg agctgcacgt cgtgccaggg
      901 cggtctgggc ccgtagcgct cgtccatctg gatcgcctgg gggtcgccga ggtggcgctc
      961 gtcgtcccgc cggccgtggg ccaggtcgat cccgcccagg aaggcgacgt cgtcgtcctc
     1021 acgcccgggg tggcggacca gcacgagctt ctggtgctgg ctgcccggcc ccctgacccg
     1081 ctcgtcgagc aggacctccc cgccggcgcg gttcacgagc tccccgaggc ggaggttctc
     1141 ctcctcgttg aagtgcgtcc ggctcgggtg ggagcgccac agcaggccgc ggacctcgac
     1201 cccgcgcgcg acgagctcgg cgagcacggt cccgagctcg gtgcccgggc cgtccaggcg
     1261 ctcgtcgggg tccccgcgcc agtcggtgac gcagacgagc tcgcccggct ggagcgagcg
     1321 gagcacggcg agcaggcgcc ggtagtaggc ggcgccgtgg accaacggct cggcgaggtt
     1381 gccctcggtc caggcgcgac cgtccccacg acggcgatcg atctccgtcg cggggttgcc
     1441 gcgctcgtcg gcccgcagga accagtcgtc cggcttcagg agccacctcc gttttccctg
     1501 tcccgttcgg cacgccgagg cgtgcctccg atttccctgt cccgttcggc acgccgaggc
     1561 gtgcctccga gcggcgtgcc tccgagctgt cgtccccaga gtgtggcgga aagtcgcggc
     1621 tccgcccaaa cccggccgtc gcccggccgc cggccggtcg tagactggcg ggcgagcggg
     1681 ctctggttcc tgacaggcac cccaggtccc acctgaccat ggacgacgag gagcgactgg
     1741 tgacggaggt gcgcccggag gccgggatcg cccgcagcgc cgcgctgttc gagcgtgccc
     1801 gggcccgcat cccgggcggg gtcaactcgc cggtccgcgc cttcggggcg gtcggcggca
     1861 cgccccgctt cttcgcgcgc ggccagggcg cctggctgtg ggacgtggac ggcaaccggt
     1921 acgtggacct ggtcctgtcc tgggggccgc tgatcgccgg gcacgcccac cccc
//
LOCUS       DASPYY010000272         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1733408, whole genome shotgun sequence.
ACCESSION   DASPYY010000272 DASPYY010000000
VERSION     DASPYY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733408"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VEP73_04185"
     CDS             <1..1469
                     /locus_tag="VEP73_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HYT25667.1"
                     /translation="PAGSRGGRLVVARNSGWQLVTFTARAVSGLAAVILVARHGGPAQ
                     LGVFQFALTLSQMFSFAVGLGLFNLLTREVAREPRTSRAWIEAGVFVALAAGGLVSAL
                     LGVGVRLAGQGPDIVEAVTLAGLALAFDTAGRISFAAFAGWERMGLESVATCLQEGLF
                     LAGVPLALDAGLGVRGVLLAYVGSRLCGALAGWLMACRELRAVMLPRPHIGFLRPTLR
                     RTIPFALDDAMSLTYIRVDSVILGFVKGPTAVGLYQAGTNLVLYLNVLARVLNTALYP
                     RMSRAWPNPRQLGRYRDVSLRLLGSVGVPIMVGSLLLAPSIFRFVYGSKFDKAVLTYQ
                     VLVLVIPIRMLGHTLGTALTAADGQTRRTVAVAAAAAANFGLNVWFIPRYSYLGAAIT
                     TAITESGLFVTYGLLLRRLAGRSALASSLAVPALASLPMAALLLAFGHGPLLLLVAAG
                     ALVYAGAVLAIALATAPSGARRRPRAALTALVSRPAPR"
BASE COUNT          141 a          611 c          525 g          244 t
ORIGIN      
        1 ccccggccgg gagccggggc ggcaggctgg tcgtcgcgcg gaactccggc tggcagctcg
       61 tcaccttcac ggccagggcg gtgagcgggc tcgccgcggt catcctcgtc gccaggcacg
      121 gcggcccggc ccagctcggc gtgttccagt tcgccctcac cctctcgcag atgttcagct
      181 tcgcggtcgg actcggcctg ttcaacctgc tcacccgcga ggtcgccagg gagcctcgca
      241 cctcgcgggc gtggatcgag gcgggcgtgt tcgtcgccct ggccgccggc ggcctcgtga
      301 gcgcgctgct gggcgtgggc gtgcggctgg ccgggcaggg cccggacatc gtcgaggcgg
      361 tcacgctcgc cggcctcgcg ctggccttcg acaccgccgg gcgcatctcc ttcgccgcct
      421 tcgccggctg ggagcggatg gggctcgaga gcgtcgccac ctgcctgcag gagggcctct
      481 tcctggccgg cgtcccgctc gcgctcgacg ccgggctcgg ggtgcgcggc gtgctgctcg
      541 cctacgtcgg cagccgcctc tgcggcgcgc tggcgggctg gctcatggcc tgccgcgagc
      601 tccgggccgt catgctgcca cggccccaca tcggcttcct gcgcccgacg ctgcgccgga
      661 cgatcccgtt cgccctcgac gacgccatgt cgctcaccta catccgcgtc gactcggtca
      721 tcctcggctt cgtgaagggc cccaccgctg tcggcctcta ccaggcgggc acgaacctcg
      781 tcctctacct caacgtgctc gcccgggtgc tgaacaccgc cctgtaccca cggatgagcc
      841 gtgcctggcc gaacccgcgg cagctggggc gctaccggga cgtgtcgctg cgcctgctcg
      901 gctcggtcgg cgtgcccatc atggtcgggt cgctgctgct cgcgccgtcg atcttccgct
      961 tcgtgtacgg cagcaagttc gacaaggccg tcctcaccta ccaggtgctc gtcctcgtca
     1021 tcccgatccg gatgctcggg cacacgctgg gcaccgcgct caccgccgcc gacggccaga
     1081 cgaggcggac ggtggcggtc gcggccgcgg ccgccgcgaa cttcggcctc aacgtctggt
     1141 tcatcccgcg ctactcctac ctgggcgccg ccatcacgac cgcgatcacc gagagcgggc
     1201 tgttcgtcac ctacgggctg ctgctgcggc ggctggccgg gcgctcggcg ctggcctcga
     1261 gcctcgcggt ccccgcgctc gcctcgctgc cgatggccgc cctgctgctc gccttcgggc
     1321 acggcccgct gctgctgctc gtcgcggccg gcgcgctcgt ctacgcgggc gccgtcctcg
     1381 ccatcgcgct cgccacggcc ccctccgggg cgcgccggcg gccccgcgcc gccctgaccg
     1441 cgctggtctc gcgcccggcc ccgaggtgac gccgatgctc ggggccgacg cgcgcctgct
     1501 gaactggcgc ctcctcgtgc c
//
LOCUS       DASPYY010000273         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2295885, whole genome shotgun sequence.
ACCESSION   DASPYY010000273 DASPYY010000000
VERSION     DASPYY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295885"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /locus_tag="VEP73_04190"
     CDS             complement(<1..74)
                     /locus_tag="VEP73_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HYT25668.1"
                     /translation="MANRYLFTSESVTEGHPDKMADQIS"
     gene            complement(256..1605)
                     /gene="coaBC"
                     /locus_tag="VEP73_04195"
     CDS             complement(256..1605)
                     /gene="coaBC"
                     /locus_tag="VEP73_04195"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018830321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HYT25669.1"
                     /translation="MPEPRGSTSSPPGPAGPGGLEGTRVLLAVTGGVAAYKAAELARE
                     LMRRGAEVQPLLTEGAEAFVTRATFAALTGRRVPATIWDEPAEVEHVALARWGDVLAV
                     VPATAHTIARLAAGLADDLVTNVALAFGGPVVVAPAMHTEMWEHPATRANVELLAARG
                     VRLVPPASGALTSGDAGPGRLADLPDLLAGVAAALGPDVGAAGRGLAGAAVRGLVGVR
                     VLVSLGGTREPLDPVRYLGNRSSGRMGAAVVAEALRRGAAVTAVAAAASVPVPPGARE
                     VRVETAQELYDAVLAEAPAHDVVVMAAAVADFRPARAAGQKLKKDHGVPELALEPTPD
                     ALAELGRVRRPGQVLVGFAAETEDHVGGGRRKLERKGLDLVVVNHVEGARSAFEADAA
                     TAYLVDPSGVEQLPDAPKAEIARRLLDRVERRLAGRAEAPRGTPGRAADRPGGPAPA"
     gene            complement(1698..1949)
                     /gene="rpoZ"
                     /locus_tag="VEP73_04200"
     CDS             complement(1698..1949)
                     /gene="rpoZ"
                     /locus_tag="VEP73_04200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYT25670.1"
                     /translation="MIEPKIDDLLARVDSKYTLVILAAKRARQINGYYSQLGEGIGEF
                     VPPMVDAGPNRKALSVALQELGEDKLNWERPADLAESIK"
     gene            complement(1946..2596)
                     /gene="gmk"
                     /locus_tag="VEP73_04205"
     CDS             complement(1946..2596)
                     /gene="gmk"
                     /locus_tag="VEP73_04205"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYT25671.1"
                     /translation="MGAASGRGSSHRRGRLIVLAGPSGVGKGAVVARLLSALPDLELS
                     VSATTRPPRPAEDEGRHYYFVDPDLFTKLVASGGFLEWAEIFGNRYGTPREPVERALG
                     EGRDVLVEIDVQGARQVRSSTPDAFMIFLKPPSLAELERRLLARGTETDEQVRRRLAK
                     ATDELAAEPEFDASVVNADLETAAREVIGIVKGLRYHRDLTTSPDGTANVPKEHPA"
     gene            complement(2602..2919)
                     /gene="mihF"
                     /locus_tag="VEP73_04210"
     CDS             complement(2602..2919)
                     /gene="mihF"
                     /locus_tag="VEP73_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HYT25672.1"
                     /translation="MPLPTLTPEQRQQALEKAAEARRKRAELKAQLKSGNLTLATVLD
                     KEDDETVGKMKVASVLESLPGVGKVRARKIMEKLDISASRRVRGLGSKQKDALLAEFG
                     TKK"
     gene            complement(3099..>3308)
                     /locus_tag="VEP73_04215"
     CDS             complement(3099..>3308)
                     /locus_tag="VEP73_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25673.1"
                     /translation="RDLAAAAGAEVVALAAIVDRRDGSSQLPVPFTALARVSAGAWEA
                     AACPSCQAGGVAESPGSRHPGGHRQ"
BASE COUNT          388 a         1210 c         1278 g          432 t
ORIGIN      
        1 gagatctggt cggccatctt gtccgggtga ccctcggtca ccgactccga cgtgaacagg
       61 tagcggtttg ccacgagcct gcctccagat gcgggtcacg acccgggcac gagcacgggc
      121 tccgggaagg gtagacggcg ccggacgctc tgggcaaacg aggccccctg ctgcccctgc
      181 gccgcccggc acgggaacga taccgtatcg gcccacctcc cgcgcgcgcc gccgggccgc
      241 cgggcggcgg gcatgctacg ccggcgccgg accgcccggc cggtcggcgg cccggccggg
      301 cgtgccgcgc ggcgcctcgg cccggccggc caggcggcgc tcgacccggt cgagcaggcg
      361 gcgggcgatc tccgccttgg gggcgtccgg cagctgctcg acaccggacg ggtcgacgag
      421 gtaggcggtg gccgcgtcgg cctcgaaggc cgaccgggcg ccctcgacgt ggttcaccac
      481 cacgaggtcg aggcccttgc gctcgagctt gcgccggccg cccccgacgt ggtcctcggt
      541 ctcggcggcg aagccgacca gcacctggcc cgggcggcgc acccggccga gctcggcgag
      601 ggcgtcgggc gtgggctcga gcgccagctc tgggacgcca tggtccttct tgagcttctg
      661 cccggcggcg cgcgcggggc ggaagtccgc gaccgcggcg gccatgacga ccacgtcatg
      721 ggccggcgcc tcggcgagga cggcgtcgta gagctcctgc gcggtctcca cccgcacctc
      781 gcgcgccccg ggcggcacgg gaacgctggc cgcggcggcg acggcggtga cggcggcgcc
      841 ccgccggagc gcctcggcga cgacggcggc gcccatccgc cccgacgacc ggttgccgag
      901 gtagcggacg gggtcgagcg gctcgcgggt gccgccgagg ctgacgagca cccgcacgcc
      961 gacgagcccc cggacggcgg cgccggcgag cccccggccg gccgcgccga cgtccgggcc
     1021 gagcgccgcg gcgacgccgg cgagcaggtc cggcaggtcg gcgaggcggc cggggccggc
     1081 gtcgcccgag gtgagcgcgc ccgaggcggg cgggacgagc cgcacgccgc gggcggcgag
     1141 cagctcgacg ttcgcgcggg tggccgggtg ctcccacatc tcggtgtgca tcgccggggc
     1201 gaccacgacc ggcccgccga acgcgagggc gacgttggtg acgaggtcgt cggcgagccc
     1261 ggcggcgagc cgggcgatgg tgtgggcggt ggccggcacg acggcgagca cgtcacccca
     1321 gcgcgccagc gcgacgtgct ccacctcggc cggctcgtcc cagatggtcg cggggacgcg
     1381 gcgcccggtc agggcggcga acgtcgccct ggtgacgaac gcctcggcac cctcggtgag
     1441 gagcggctgt acctcggcgc cgcggcgcat cagctcgcgg gcgagctcgg cggccttgta
     1501 ggcggcgacg ccgccggtga ccgcgagcag cacccgggtc ccctcgagcc cgcccggccc
     1561 cgcggggccg ggcgggctcg aggtgctgcc tcgcggctcc ggcatcgtcg tcctgggctt
     1621 tggctggaga gcagtgaacg cgggcgcagc ccgctccgct ccggggggga gctcgggggc
     1681 ctggcccccc gaggctgcta cttgatggac tcggccaggt cggccgggcg ctcccagttc
     1741 agcttgtcct cgccgagctc ctggagcgcg acggagaggg ccttgcggtt cgggccggcg
     1801 tcgaccatgg gcggcacgaa ctcgccgatg ccctcgccga gctgggagta gtagccgttg
     1861 atctgccgtg cgcgcttggc ggccaggatg accagggtgt acttcgagtc gacccgggcg
     1921 agcaggtcgt cgatcttcgg ttcgatcacg cggggtgctc cttggggacg ttcgccgtgc
     1981 cgtcaggtga tgtcgtcagg tcgcgatggt accgcaggcc cttgacgatg ccgatcacct
     2041 cgcgggcggc ggtctcgagg tcggcgttca ccaccgaggc gtcgaactcc ggctcggcgg
     2101 cgagctcgtc ggtcgccttg gccaggcgcc tgcggacctg ctcgtcggtc tcggtgcctc
     2161 tggccagcag ccgccgctcg agctcggcca ggctgggcgg cttgaggaag atcatgaagg
     2221 cgtcgggcgt gctcgaccgg acctgccgcg cgccctgcac gtcgatctcg acgagcacgt
     2281 cgcgcccctc cccgagcgcc cgctcgaccg gctcccgcgg cgtgccgtag cggttgccga
     2341 agatctcggc ccactcgagg aacccgcccg aggcgaccag cttggtgaac aggtcggggt
     2401 ccacgaagta gtagtggcgg ccctcgtcct cggccggcct gggcggccgg gtggtcgccg
     2461 agaccgacag ctcgaggtcg ggcagcgcgg agagcagcct ggccacgacg gctcccttgc
     2521 cgaccccgga gggaccggca aggacgatca ggcggccacg gcggtgggac gatcctctgc
     2581 cagacgcggc gcccagggtg gttacttctt ggtgccgaac tcggcgagca gggcgtcctt
     2641 ctgcttggag ccgaggcccc ggacgcgccg ggacgccgag atgtccagct tctccatgat
     2701 cttgcgggcg cgcaccttgc cgacgccggg caggctctcg agcacgctgg cgaccttcat
     2761 cttgcccacg gtctcgtcgt cctccttgtc gaggacggtg gcgagggtga ggttgccgct
     2821 cttcagctgc gccttgagct cggcccgctt gcgccgggcc tcggccgcct tctccaaggc
     2881 ctgctggcgc tgctctggcg tcagggtggg cagtggcatg gtgcctcctc gtgacggggt
     2941 ggcctgggcg gacgaccgcg gcgctccccg atgccggttt tcaagaacgc gccggattct
     3001 acgcacacca ggggggcggc cacaatccat cggcgcaggt cagaacgccg cctctgtaga
     3061 tttcccgaca tgtcggacgg ccgggcacgg gcttccggtc actgccggtg gccgcccggg
     3121 tgtctcgaac cgggcgactc ggctaccccg ccggcctggc aggacgggca cgccgccgcc
     3181 tcccaggccc ctgccgagac cctcgcgagc gccgtgaacg ggactgggag ctgcgaggac
     3241 ccgtcccggc ggtcgacgat ggccgccagc gcgaccacct cggcgccggc cgcggcggcc
     3301 aggtcgcg
//
LOCUS       DASPYY010000274         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_610319, whole genome shotgun sequence.
ACCESSION   DASPYY010000274 DASPYY010000000
VERSION     DASPYY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610319"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VEP73_04220"
     CDS             <1..216
                     /locus_tag="VEP73_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HYT25674.1"
                     /translation="VLVGDTPLDVAAALAGGARVVAVATGHYGMEELEAAGADVVLPD
                     LRDTPAVLRAVLGGTGATPDGPAASAC"
     gene            complement(213..890)
                     /locus_tag="VEP73_04225"
     CDS             complement(213..890)
                     /locus_tag="VEP73_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HYT25675.1"
                     /translation="MPWRREVPGGMAPDRSTRLSGTARIEAFSDGVMAIAITLLILEI
                     RVPPQSGSLLAGLGRLWPSYLAYLASFFTIGIIWLNHHAFFGRLRHVDHVLQWWNLIL
                     LLMVSFLPFPTSVVAEYIRQGSASDARVAVALYGVVGVLMTVPWVFLWRRLVRRPELF
                     EPPYDADFARAEGGRSWVGIVVYAACIGVGLLLPVAALALYLAVAVFYAVTSQGWSSA
                     RAATPVD"
     gene            1046..2092
                     /locus_tag="VEP73_04230"
     CDS             1046..2092
                     /locus_tag="VEP73_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYT25676.1"
                     /translation="MRAITLVPGRAGSVRLDEVEEPSPSYGPVLVDTIAMGVCGTDAE
                     IVRGDYGWAPPGRERLILGHESLGRVAEAPEGSGLAAGDLVVGIVRRPDPVPCESCAV
                     GEWDMCRNGRYTERGIKELDGFGSERYRVEPGFAIRLDPGLERVGVLLEPASVLAKAW
                     EQVERIGARSHWTPKRALVTGAGPIGLLAALLGVQRGLEVHALDQVTGGPKPGLVADL
                     GATYHTGSLAGACPDPDVVVECTGVGQLVFDAMTALAPDGIVCLIGVSAAGRSVQVDA
                     GAINRELVLENNAIVGSVNANRRHYEAAAKALDEADEGWLERLITRRVPLERFSEALQ
                     RRPGDVKVVVDFTR"
     gene            complement(2129..>2670)
                     /locus_tag="VEP73_04235"
     CDS             complement(2129..>2670)
                     /locus_tag="VEP73_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYT25677.1"
                     /translation="VPTPLAARLRDGSASGVWELDPRRSSVEFRVKHFWGMVTVRGQF
                     TSFQGAATIDPSGAISGTMRIDAASVESRQARRDAHLRSADFFHADDHPTISFQTRQV
                     SPRDDDRVRVVGDLTAAGRTRPIEFEARVTDATADRLRAVAEITIDRTEFGMTWSPMG
                     MTSSEVLVVVQGELARSST"
BASE COUNT          373 a          956 c         1009 g          332 t
ORIGIN      
        1 gtgctggtcg gcgacacccc gctcgacgtc gcggccgccc tcgccggcgg cgcccgggtg
       61 gtggcggtcg ccaccgggca ctacggcatg gaggagctgg aggcggccgg cgccgacgtg
      121 gtgctgcccg acctccgcga cacgcccgcc gtgctgcggg ccgtcctcgg cgggaccggg
      181 gcgacgcccg acggcccggc ggcgtcggcg tgctagtcga cgggcgtggc ggcccgcgcc
      241 gagctccagc cctggctggt gacggcgtag aacacggcga ccgcgagata cagcgccagg
      301 gccgccaccg gcaggagcag accgacaccg atgcatgccg cgtagacgac gatgccgacc
      361 caggagcggc ctccctcggc ccgggcgaag tcggcatcgt agggcggctc gaacagctcg
      421 ggccggcgga ccagccgccg ccacaggaac acccacggca cggtcatgag cacaccgacc
      481 acgccgtaga gggcgaccgc cacccgggcg tcgctggcgc tgccctggcg gatgtactcg
      541 gccaccaccg acgttgggaa cggcaggaac gacaccatca gcagcagtat caggttccac
      601 cactgcagca cgtggtcgac gtggcgcagc cggccgaaga aggcatggtg gttgagccag
      661 atgatgccga tcgtgaagaa cgacgcgagg tacgccaggt acgacggcca cagccggccc
      721 aggccggcga gcagcgaccc gctctgcggc ggcacccgta tctcgaggat gagcagggtg
      781 atcgcgatcg ccatgacgcc gtcgctgaac gcctcgatac gggcggtacc ggacagtcgc
      841 gtggatcgat caggcgccat gccacccgga acctcccgac gccacggcat attcctctta
      901 cgagcggctc ccggacctgg cccgggcccg gggtcctcgg tgacgcgacc ggctggattg
      961 cggccggcgc cgggtgggaa tcggaagggc gtccctgcgg atgcggccgc cggccgagag
     1021 ccggccgaga gtaggaggcg ggcggatgcg ggcgatcacc ctggtgccgg gccgggcggg
     1081 cagcgtccgc ctcgacgagg tcgaggagcc gtccccgtcg tacgggccgg tgctggtcga
     1141 caccatcgcg atgggggtct gcggcaccga cgcggagatc gtccgcggcg actacggctg
     1201 ggcgccgcca ggccgcgagc ggctcatcct cgggcacgag tccctcggcc gggtcgccga
     1261 ggcgcccgag ggcagcgggc tggccgccgg ggacctggtc gtcggcatcg tccgccgccc
     1321 ggacccggtg ccctgcgaga gctgcgcggt cggcgagtgg gacatgtgcc gcaacggccg
     1381 gtacaccgag cggggcatca aggagctgga cggcttcggc tccgagcggt accgggtcga
     1441 gcccgggttc gcgatccggc tcgacccggg gctcgagcgg gtcggtgtgc tcctcgagcc
     1501 ggccagcgtc ctcgccaagg cgtgggagca ggtcgagcgc atcggcgcgc ggtcccactg
     1561 gacgccgaag cgggcgctcg tgaccggcgc cgggccgatc ggcctgctcg ccgccctgct
     1621 cggggtgcag cgggggcttg aggtgcacgc gctcgaccag gtgaccgggg gcccgaagcc
     1681 cgggctggtc gccgacctcg gcgccaccta ccacaccggg tcactggccg gcgcctgccc
     1741 cgatcccgac gtcgtcgtcg agtgcaccgg tgtcgggcag ctcgtcttcg acgccatgac
     1801 cgcgctcgca cccgacggca tcgtctgcct catcggggtc tcggcggccg gccgctccgt
     1861 ccaggtggac gccggcgcca ttaaccgcga gctggtgctc gagaacaacg cgatcgtggg
     1921 gtcggtgaac gccaaccggc gccactacga ggccgccgcc aaggcgctcg acgaggcgga
     1981 cgaaggctgg ctcgagcggc tcatcacccg ccgggtgccg ctggagcggt tctccgaggc
     2041 gctccagcgc cggcccggcg acgtcaaggt ggtcgtcgac ttcacccggt gagcgttgcc
     2101 ggcgcccggt cgcccgcccg ctgcggcgtc aggtcgagga cctggcgagc tcgccctgga
     2161 cgaccaccag cacctcggac gaggtcatgc ccatgggact ccaggtcatg ccgaactcgg
     2221 tgcggtcgat cgtgatctcg gcgacggccc tcaggcggtc ggccgtcgcg tcggtgacgc
     2281 gcgcctcgaa ctcgatcggc cgcgtgcgcc ccgcggcggt caggtcgccg accacccgca
     2341 cgcggtcgtc gtcccgcggg ctgacctggc gggtctggaa cgagatcgtg gggtggtcgt
     2401 cggcgtggaa gaagtcggcc gaccgcaggt gggcgtcgcg cctcgcctgc ctggactcca
     2461 cggaggcggc gtcgatccgc atcgtcccgg agatggcgcc ggatgggtcg atggtggccg
     2521 ctccctggaa gctcgtgaat tgcccgcgga cggtcaccat cccccagaag tgcttgacgc
     2581 ggaactccac gctggatcgc cgcggatcca gctcccagac accggacgcc gacccgtcgc
     2641 gcagccgggc cgccagcggg gtggggactc
//
LOCUS       DASPYY010000275         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2014852, whole genome shotgun sequence.
ACCESSION   DASPYY010000275 DASPYY010000000
VERSION     DASPYY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014852"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VEP73_04240"
     CDS             complement(<1..554)
                     /locus_tag="VEP73_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25678.1"
                     /translation="MTIRVALADDQPLVLAGLRAMVAFAPDLELVGEAADGSQAVALA
                     GRERPDVLLMDVRMPGLDGIEATRRITGAEGTAGVRILILTTFELDEYVYAALRAGAS
                     GFLLKDARPEDIFAAIRVVAAGEALLAPRVTRRLIEEFARRPGAAGVARTALDPLTGR
                     EREVLALVARGLSNGEIAERLVVSP"
     gene            complement(559..1758)
                     /locus_tag="VEP73_04245"
     CDS             complement(559..1758)
                     /locus_tag="VEP73_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYT25679.1"
                     /translation="MSMRASRHGWLRPLGPSAFDAALAVVAAGIGLALLASMAGAVAH
                     AGAAGTAVAAYVLVLLHTLPLALRRRRPLLVLWSGLATGLAVAAIGFPLVVLGPAILV
                     PIYTVAARCGRRVSLAGVAAVEAAALLAELLHPGVVGDRSTLATLALVSGTVWLLGDS
                     ARRREAAAATAEERAVALQRAREELADRAVAAERLRIARELHDVVAHSMSVIAVQSGT
                     GRLVIDARPDEARRALEAIEVTSRAALAEMRQLLGVLRQDGDAGVLVPLPGLGDLDAL
                     VAQVVGSGLAVEVRVEGTRSGVPAGVDLSAYRIVQEALTNVLKHAGPATATVVVRYGD
                     GEVAVEVTDDGRPAAPVPARQDLTRHGTVGMRERAALYGGRLEAGPTTGGGWRVAARL
                     PFGEPRT"
     gene            1901..>2350
                     /locus_tag="VEP73_04250"
     CDS             1901..>2350
                     /locus_tag="VEP73_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25680.1"
                     /translation="MRTMRLGGLAVLAALALLLAACGGKAKEPSGQPPASQAQSATAV
                     RVAGSQLGKVVTDDKGRTLYALKSDQGGKSTCYGTCARTWPPLVAKGSPAGGDGLTAS
                     LLGTAARNDGGMQVTYKRRPLYYYSGDQRPGDTNGQGIGNVWFAVGAD"
BASE COUNT          316 a          924 c          880 g          230 t
ORIGIN      
        1 gggctgacga ccaggcgctc ggcgatctcg ccgttcgaca ggccgcgggc cacgagggcg
       61 agcacctcgc gctcccggcc ggtgagggga tcgagcgcgg tccgtgccac cccggccgcg
      121 ccgggccggc gcgcgaactc ctcgatcagc cggcgcgtca cccgcggggc cagcagcgcc
      181 tcgccggcgg cgacgacccg gatcgcggcg aagatgtcct cgggccgggc gtccttcagc
      241 aggaacccgc tcgcccccgc ccgcagcgcg gcgtagacgt actcgtcgag ctcgaacgtg
      301 gtgaggatga ggatccggac cccggcggtg ccctccgcgc cggtgatgcg cctggtcgcc
      361 tcgatgccgt ccagccccgg catgcggacg tccatgagca gcacgtcggg ccgctcccgc
      421 ccggccagcg cgaccgcctg cgagccgtcg gcggcctcgc cgaccagctc gaggtcgggc
      481 gcgaacgcga ccatggcgcg gagcccggcc agcaccagcg gctggtcgtc ggcgagcgcg
      541 acccggatgg tcacgccctc acgtccgggg ctccccgaac ggcaggcggg ccgccacccg
      601 ccagcccccg ccggtcgtcg gaccggcctc gagccggccg ccgtagaggg cggcccgctc
      661 ccgcatcccg acggtgccgt gcctggtcag gtcctgccgg gcgggaaccg gcgcggccgg
      721 gcgcccgtcg tcggtgacct ccaccgccac ctcgccgtcg ccgtagcgga cgacgaccgt
      781 ggccgtcgcg ggcccggcgt gcttgagcac gttggtcagg gcctcctgca cgatgcggta
      841 cgcagagagg tcgaccccgg ccggcacgcc ggaccgcgtc ccctcgaccc ggacctcgac
      901 ggccagcccg gacccgacca cctgcgcgac gagggcgtcg aggtcgccga ggcccggcag
      961 cgggaccagg acgcccgcgt cgccgtcctg cctgagcacc ccgaggagct ggcgcatctc
     1021 cgcgagcgcg gcccggctgg tcacctcgat cgcctcgagc gcccggcggg cctcgtcggg
     1081 acgggcgtcg atcaccaggc ggccggtgcc cgactgcacg gcgatcacgc tcatgctgtg
     1141 cgccaccacg tcgtgcagct cccgggcgat ccgcagccgc tcggcggcga ccgcgcggtc
     1201 ggcgagctcc tcccgcgccc gctggagcgc caccgcccgc tcctcggccg tggccgcggc
     1261 ggcctcgcgt cggcgggcgc tgtcgccgag cagccacacc gtaccggaca ccagcgccag
     1321 ggtcgccagc gtggagcgat ccccgaccac ccccggatgg agcagctcgg ccagcaacgc
     1381 cgcggcttcc acggccgcca cacccgcgag cgacacgcgc cggccgcagc gggccgccac
     1441 ggtatagatg gggacgagga tggccgggcc gagcaccacc agcgggaagc cgatggccgc
     1501 gaccgcgagg ccggtcgcga ggcccgacca cagcacgagc aggggacggc ggcgccggag
     1561 ggcgaggggc agcgtgtgga gcaggacgag cacgtacgcc gcgacggcgg tcccggcggc
     1621 gccggcgtgg gcgacggcgc cggccatcga agccaggagc gcgaggccga tcccggccgc
     1681 gacgaccgcc agcgcggcgt cgaacgcgga cggccccagg gggcggagcc atccatggcg
     1741 cgatgcccgc atgctcatgg ggtcgacagt aaccggcggc ccgtcccggc gcatccatcc
     1801 cggcgcggtc ggatcggcgg gaagcgcccg ggaggtactg gagttctcca gagcgaaggc
     1861 ccgctcggcc cgcggagccc gcggagaagg agaggatcac atgcgcacca tgcgtctcgg
     1921 cggcctcgcc gtgctcgccg cgctcgcgct cctgctggcc gcctgcggcg ggaaggcgaa
     1981 ggagccgtcc gggcagccgc cggcctccca ggcgcagtcg gcgaccgcgg tccgggtggc
     2041 agggtcccag ctcggcaagg tcgtcaccga cgacaagggc cgcaccctgt acgcgctcaa
     2101 gtccgaccag ggcggcaaga gcacctgcta cggcacctgc gcgcggacct ggccgcccct
     2161 ggtggccaag gggagcccgg ccggcggcga cggcctgacc gcgtccctgc tcggcacggc
     2221 ggcccgcaac gacggcggca tgcaggtgac ctacaagcgc cggccgctct actactactc
     2281 gggcgaccag cgacccggcg acaccaacgg ccagggcatc ggcaacgtct ggttcgcggt
     2341 gggcgccgac
//
LOCUS       DASPYY010000276         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_610344, whole genome shotgun sequence.
ACCESSION   DASPYY010000276 DASPYY010000000
VERSION     DASPYY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610344"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEP73_04255"
     CDS             <1..358
                     /locus_tag="VEP73_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYT25681.1"
                     /translation="WSIPKGEYGDGDDPLQAALREFEEETGHPPPQAGLRELGAIRQR
                     GGKVLTVWAAAGDLDAATIASNSFTLEWPPRSGALREFPEVDRAGWFDLDSAREKLLS
                     AQGELLDRLLATLPQH"
     gene            481..1716
                     /locus_tag="VEP73_04260"
     CDS             481..1716
                     /locus_tag="VEP73_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25682.1"
                     /translation="MPGRDPARRPRAVLAPEISEIAGSVAARLPDLVDRVVRRIVAEF
                     DFYGASDVVSLEDLRESVRGNVESMVGQLTTGQPPDLSAPRATGRRRAQQGALLAELL
                     HAYRIGFTELWGAIVDEARRSTRSPPEMLVDAASSVWWLIGEYTEELTMAYREAAAEL
                     LLAGARERSALVEALFTGGIPDRGTLWEAAKLLRLPWEGVFAVVAVEAPGLAQEGLPD
                     AEAFLAARGIGSAWRLHPDTQTGVVSLRHHDTAPLLLELLGRGVRARTGVSPVYHSLG
                     DTPRALHYARLVLSTLPPGAPAVAQFEETPLRVLAAAAPDAAGELARTVLGPVLDLPV
                     QDRSTLLGTLRAWFDAGGSAVETGKRIYCHPNTVRYRLRRLQEHTGRSLDDPRAVAEL
                     LAALEALPLVEEASRTVER"
BASE COUNT          223 a          644 c          637 g          228 t
ORIGIN      
        1 ctggtcgatt cccaagggcg agtacgggga cggcgacgac ccgctgcagg ccgccctgcg
       61 ggagttcgag gaggagaccg ggcacccgcc cccccaggcg ggcctgcgcg agcttggcgc
      121 gatccggcag cggggcggga aggtcctcac cgtctgggcg gcggcgggcg acctcgacgc
      181 cgccacgatc gcgagcaaca gcttcacgct cgagtggcca ccgcgctccg gcgccctgcg
      241 ggagttcccg gaggtcgacc gtgccggctg gttcgatctc gacagcgccc gggagaagct
      301 gctcagcgcg cagggcgagc tgctcgaccg gctgctcgcg acgctgcccc agcactgaca
      361 cacgggctcg ggcgcgtcgt cgggacgggc ggccagccgc cccaaactcg aagggcgctg
      421 gcgaagcggc gttgcgggtt gacaggcggt cgtggggacg taccttcacc catgatgagc
      481 atgcccggcc gtgaccccgc gcgccgcccg cgcgccgtcc tggctcccga gatctccgag
      541 atcgcagggt cggtcgcggc acgccttccc gacctcgtcg accgggtcgt ccggcgcatc
      601 gtggcggagt tcgacttcta cggcgcgagt gacgtcgtca gcctcgagga ccttcgggag
      661 tcggtccgcg gcaacgtcga gtccatggtc ggccagctca ccaccgggca gccgcccgac
      721 ctgtccgcgc ctcgcgccac cggtcgccgc cgcgcacagc agggcgcact cctggcggaa
      781 ctcctccatg cgtaccggat cggcttcacc gagctgtggg gcgcgatcgt cgacgaggct
      841 cgccggagca cccggtcgcc gcccgagatg ctcgtggacg cggccagcag cgtgtggtgg
      901 ctcatcggcg agtacaccga ggaactcacc atggcgtacc gggaagccgc ggcggagctg
      961 ctgctcgccg gcgcgcggga gcgttccgcc ctggtggagg cgttgttcac cggcggcatc
     1021 cccgacaggg gcaccctctg ggaggccgcg aagctgctca ggctcccctg ggaaggggtc
     1081 ttcgccgtgg tggccgtcga ggcgcccggg ctggcccagg aagggctgcc ggacgccgag
     1141 gcattcctgg ccgcgcgcgg gatcgggtcg gcctggcggc tgcatcccga cacccagacg
     1201 ggtgtggtgt cgctgcggca ccacgacacg gcgcccctcc tcctcgagct gctgggacgc
     1261 ggcgtccgcg cccggaccgg cgtcagcccc gtctaccact cgctcggtga cacaccccgc
     1321 gccctgcact acgcgcgact ggtcttgagc acgctgccgc caggagcccc ggccgtcgcc
     1381 cagttcgagg agacaccgct tcgcgtgctc gccgccgccg ccccggacgc ggccggagag
     1441 ctcgcgcgca cggtgctcgg gccggtcctc gacctgcccg tgcaggaccg ctcgacgctg
     1501 ctcggcacct tgcgggcgtg gttcgacgcc ggcggctccg cagtcgagac cgggaagcgg
     1561 atctattgcc atcccaacac ggtgcggtac cggctgcgac ggctgcagga gcacaccggc
     1621 cggtccctgg acgacccgag agccgtggcc gagctgctcg ccgccctgga ggcgctgccc
     1681 ctcgtggagg aggcgagccg gacggtcgag cgctgagcgt cgcgccgccc gg
//
LOCUS       DASPYY010000277         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_47237, whole genome shotgun sequence.
ACCESSION   DASPYY010000277 DASPYY010000000
VERSION     DASPYY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47237"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VEP73_04265"
     CDS             <1..704
                     /locus_tag="VEP73_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25683.1"
                     /translation="TSLTLFAAAHADLWIVGALRPHREVAVYGVAARAATVVAMPLLV
                     VNAVLPPVVAELHAQGRRDELERVLRGAAALAGLPALAALAALAIAGGPLLGLMYGHA
                     YRAGGAVLTLLACGQAAVVCTGSCGLTLAMTGRQALLATATGIGGGLTVVAAALAARP
                     FGTTGVAAATAAGLVLQNLAMLVAVRLTAGVWTHLSLAPLLGAARAFRAGGRVPAADG
                     GRVPAADGRAAAHER"
     gene            694..1677
                     /locus_tag="VEP73_04270"
     CDS             694..1677
                     /locus_tag="VEP73_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYT25684.1"
                     /translation="MSGERVRVSGDPVKVLYIEGAGRSGSTVLDNLLGQLPGFFSVGE
                     LRFVWDRNLLEDRLCGCGRPFGSCPVWSEVVGRAFGGGAVDPHEMIRLREGAVRLRDT
                     PLAATRWAERLLRPRSAGYLDVLARLYPAVAGVTGARVVVDSSKYPSYGLLLGMLPTV
                     DLYVLHLVRDPRGVAHSLRRVKPEPVARGVFEEMQRTGPARSAVDWSLCNLLAETFAR
                     RARDRYLPVRYEDFVAEPRATVLRILGHVGEPARELPFVGERAVRLGVNHTVSGNPSR
                     FRTGTVELRPDDEWRTRGRPAGRALVTALCLPLLARYGYVGRGRRRRTHPA"
BASE COUNT          164 a          657 c          645 g          223 t
ORIGIN      
        1 tgaccagcct gaccctgttc gcggccgccc acgcggacct ctggatcgtg ggggcgctcc
       61 gcccccaccg ggaggtcgcg gtctacgggg tggccgcccg cgcggccacc gtcgtcgcga
      121 tgccgctgct cgtcgtcaac gccgtgctcc cgccggtcgt ggccgagctg catgcgcagg
      181 gccgccggga cgagctcgag cgcgtcctgc gcggcgcggc ggccctggcc ggcctgcccg
      241 ccctcgccgc cctcgccgcc ctcgccatcg ccggcgggcc gctcctgggg ctcatgtacg
      301 gccacgccta ccgggccggc ggggccgtcc tgaccctgct ggcctgcggc caggccgccg
      361 tcgtctgcac cgggtcgtgc gggctgacgc tcgcgatgac cggccgccag gcactgctgg
      421 cgaccgcgac cggcatcggc ggcggcctca cggtggtcgc ggcggccctc gccgccaggc
      481 cgttcggcac gaccggcgtc gccgccgcca ccgcggccgg gctggtcctc cagaacctcg
      541 cgatgctcgt cgcggtacgg ctgaccgcgg gcgtgtggac ccacctctcg ctcgccccgc
      601 tgctgggggc ggcgcgagcg ttccgggccg gtggcagggt cccggcggcc gacggtggca
      661 gggtcccggc ggccgacggg agggccgccg cgcatgagcg gtgagcgggt gcgcgtgagc
      721 ggtgaccccg tgaaggtgct ctacatcgag ggggccggcc gcagcggcag cacggtgctc
      781 gacaacctcc tcgggcagct cccagggttc ttctcggtcg gggagctgcg cttcgtctgg
      841 gaccggaacc tgctcgagga ccggctgtgc ggctgcggcc ggccgttcgg gtcctgcccg
      901 gtgtggtcgg aggtggtcgg ccgtgccttc ggcgggggcg ccgtcgaccc gcacgagatg
      961 atccggctcc gggaaggcgc cgtgcgcctg cgcgacaccc ccctcgcggc gacccgctgg
     1021 gcggagcggc tgctccggcc gaggagcgcc ggctacctcg acgtcctggc acgcctgtac
     1081 cccgccgtcg ccggcgtgac gggcgcccgg gtggtggtcg actcgtcgaa gtacccgtcg
     1141 tacggcctcc tcctcgggat gctgccaacc gtcgacctgt acgtcctcca cctggtcagg
     1201 gacccgaggg gcgtggcgca ctccctgcgc cgggtgaagc cggagccggt cgccaggggc
     1261 gtcttcgagg agatgcagcg gaccggaccc gccaggtcgg cggtcgactg gagcctgtgc
     1321 aacctgctcg cggagacgtt cgcgcgccgc gcccgcgacc gctacctccc cgtccgctac
     1381 gaggacttcg tcgccgagcc gcgcgccacg gtgctgcgca tcctcgggca cgtgggcgag
     1441 cccgcccggg agctcccgtt cgtgggggag cgcgccgtcc ggctcggcgt caaccacacc
     1501 gtcagcggga acccgagccg cttccggacc gggaccgtcg agctgcgacc cgacgacgag
     1561 tggaggacgc gcggccggcc ggccggccgc gcgctcgtca ccgcgctctg cctgcccctg
     1621 ctggcgcggt acggctacgt cggccgcggc cggcggcgcc ggacgcaccc ggcctgacgc
     1681 ctgcgcggc
//
LOCUS       DASPYY010000278         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_470819, whole genome shotgun sequence.
ACCESSION   DASPYY010000278 DASPYY010000000
VERSION     DASPYY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470819"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1928)
                     /locus_tag="VEP73_04275"
     CDS             complement(<1..>1928)
                     /locus_tag="VEP73_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25685.1"
                     /translation="EGGPGPGRDPELAPPCPETAGRPPSREPGTARRGPDAGGAAADP
                     AGVRAGRDQASSGHPRARPVPDRAPGPALGRVAAAILVAAVAFLASALWAQRSPDVGD
                     GEAAIVQVARGTAQAGGLAWHGGPVADRPPLALAAHGGWLSLTGRVTGQVVSSVEASR
                     LASSAFRALAIVVLVVLVLALTDPRQHAALRFLVGIAAGLLAAVDPVLVGAGRTVTLE
                     APGLLTALLVLWLAWLLRDRPPAVYVPVVGLGSGVALLTDGRTVVLLAVPLVFGVLAR
                     ARGEAFAGRALAALLVGAGFWAAFAAWALAAAPGTAGWPHRLAVLVHLAGSEQSAPLG
                     RPLALTAPRDLATLIVLAAALPGLAALRRWRSDRASLYVLAWNLAGLGAGAALLAGGA
                     LDESWLTYLLPGAIVAAAAGFEAARTRLSTGRRLLGGSSAAVAMLLACALLAVAGAGW
                     ERRYGHADEALARMSGVVAGGVQACSALNASSTADADLFSIGGRRVTQFSSGPAAVAH
                     GVRYFLLRSDDVAERRGTMSGGLPDWLRSTGRPVAAIPSPSHGSVQLWRVSQPATSRV
                     ADLLRVPGGVFENVLGSGCGGYAVVDAHTGRFFSGYQALGGKPVIGRPLSSPWEASGS
                     TMQAFDTMMLRTVPDPGG"
BASE COUNT          261 a          756 c          758 g          153 t
ORIGIN      
        1 cccgccaggg tcgggcaccg tacggagcat catcgtgtcg aacgcctgca tggtggaccc
       61 gctcgcctcc caggggctgc tgagcggacg gccgatcacg ggcttgcccc cgagcgcctg
      121 gtagccggag aagaaccggc ccgtgtgggc gtcgaccacg gcgtagccgc cgcagccgga
      181 gccgagcacg ttctcgaaca cgccgcccgg gacccgcagc aggtcggcga cccggctcgt
      241 ggccggctgg ctcacccgcc agagctggac gctgccgtgg ctcgggctgg ggatggcggc
      301 caccggccgg cccgtcgacc gcagccagtc gggcaggccg cccgacatcg tcccccgccg
      361 ctcggcaacg tcgtcggacc tgagcaggaa gtagcgcacg ccgtgggcca cggcggccgg
      421 accgctcgag aactgggtca cgcggcgccc gccgatcgag aagaggtcgg cgtcggccgt
      481 gctggacgcg ttgagcgccg agcaggcctg cacgccgccc gccacgaccc cgctcatccg
      541 cgccagcgcc tcgtcggcgt gcccgtaccg cctctcccaa cccgcccccg ccaccgcgag
      601 cagcgcgcat gcgagcagca tggcgaccgc cgccgaggac cctccgagca ggcgccgccc
      661 ggtgctcagc ctggtgcggg ccgcctcgaa tcctgcggcg gccgcgacga tcgccccggg
      721 cagcaggtag gtgagccacg actcgtcgag ggcgccgcca gcgagcagcg cggcgccggc
      781 cccgagcccg gccaggttcc aggccaggac gtacaggctg gcgcggtcgg agcgccagcg
      841 ccgcagggcg gccaggccag gcagggccgc cgccagcacg atcagggtgg cgaggtcgcg
      901 aggtgcagtg agcgcgagcg gacggccgag gggcgccgac tgctcggagc cggcgaggtg
      961 cacgaggacg gccagccggt gcggccagcc cgccgtgccc ggcgccgcgg cgagggccca
     1021 ggccgcgaac gccgcccaga acccggcccc gacgagcagg gcggcgagcg cccgaccggc
     1081 gaaggcctcc ccccgggcac gggcgagcac gccgaacacc agcggcacgg cgagcaggac
     1141 gacggtgcgg ccgtcggtga ggagggcgac gccggacccg aggccgacca ccgggacgta
     1201 gaccgcgggc gggcggtcac ggagcagcca ggccagccac aggaccagca gcgcggtgag
     1261 gagccccggc gcctcgaggg tcaccgtccg gccggcgccg acgaggaccg ggtcgacggc
     1321 cgccagcagg ccggccgcga tcccgacgag gaagcggagg gccgcgtgct gcctcgggtc
     1381 ggtgagggcg agcacgagca ccaccagcac gacgatcgcg agggcgcgga aggcgctgga
     1441 cgcgagccgg ctcgcctcga cggacgagac cacctggccc gtgacccgcc cggtgagcga
     1501 gagccagcca ccgtgggccg ccagggcgag cgggggccgg tcggccaccg gccccccgtg
     1561 ccacgcgagc ccgcctgcct gggcggtgcc acgggccacc tggacgatgg cggcctcgcc
     1621 gtcaccgacg tcagggctcc gctgcgccca gagcgcggac gcgaggaagg cgacggccgc
     1681 gaccaggatg gcggccgcca cccgcccgag cgcgggaccg ggcgcccggt ccgggaccgg
     1741 gcgcgcccgg gggtgcccgg agctggcctg gtccctgccc gcccgcaccc cggcggggtc
     1801 ggccgccgcc ccgcctgcat ccggcccccg tcgcgccgtg ccgggctccc tggacggggg
     1861 ccggcccgcg gtctccgggc acggcggggc gagctcggga tcccggcccg ggccgggacc
     1921 tccttccg
//
LOCUS       DASPYY010000279         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_610518, whole genome shotgun sequence.
ACCESSION   DASPYY010000279 DASPYY010000000
VERSION     DASPYY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610518"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="moaC"
                     /locus_tag="VEP73_04280"
     CDS             <1..459
                     /gene="moaC"
                     /locus_tag="VEP73_04280"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYT25686.1"
                     /translation="MVDVSAKPESERVAVAGCRVLLAPATLELLATGGLPKGDALAVA
                     RIAGIMGAKRTPELIPLCHPLPLSGVEVGLELEDDAVAVTATVRTTARTGVEMEALTA
                     AATAALALYDMVKGVQRDARIDGLRLLAKRGGRSGDYRVPDALDAERGDG"
     gene            452..967
                     /locus_tag="VEP73_04285"
     CDS             452..967
                     /locus_tag="VEP73_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HYT25687.1"
                     /translation="MAEGLRGIRVAVLTVSDRVAEGSRGDAGGDALRALLQEAGATLT
                     AREVVPDEQARIAATLRSLAAAADVVLTTGGTGLGPRDVTPEATREVLDREAPGIPEA
                     LRHASLAITPFAMLSRATAGLAGHTLIVNLPGNPKAVGEEWEVLAPILAHAVATAQGP
                     VPDASHQHLKR"
     gene            1085..1492
                     /locus_tag="VEP73_04290"
     CDS             1085..1492
                     /locus_tag="VEP73_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25688.1"
                     /translation="MGRAFPHQQLKRGFVVSVPADRHRQMVARRRRVLVILSIASLAG
                     LRAWVALGGQWWLAAAITGSLLTGYLVALAAAGRRRHVEEPQASQAAAPAVAPPTIAA
                     SVPAEALPLPEPAGERASRQRRPLSRRLRPVAK"
     gene            1489..>1755
                     /locus_tag="VEP73_04295"
     CDS             1489..>1755
                     /locus_tag="VEP73_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HYT25689.1"
                     /translation="MSFAEIQRGLHDRFDAKHRAREVGIGNARQAIRRSANAIRAVHR
                     AELEVADRLMVEARELLDIAESVLAAHPDAYYAGFLQDAQKEYAE"
BASE COUNT          225 a          655 c          634 g          241 t
ORIGIN      
        1 atggtcgatg tctcggccaa gccggagagc gagcgggtcg cggtggccgg gtgccgcgtg
       61 ctgctcgccc cggccacgct ggagctgctc gccaccggcg ggctgcccaa gggcgacgcg
      121 ctggcggtcg cccggatcgc cgggatcatg ggcgcgaagc gcacccccga gctgatcccg
      181 ctgtgccatc cgctgccgct cagcggcgtg gaggtcggcc tcgagctgga agatgacgcg
      241 gtcgccgtca ccgccacggt ccgcaccacc gcccgcaccg gcgtggagat ggaggcgctc
      301 actgccgccg ccactgccgc gctcgccctc tacgacatgg tcaagggcgt gcagcgcgac
      361 gcccgcatcg acgggctgcg gctgctcgcc aaacgcggcg gccgctcggg cgactaccgc
      421 gtcccggacg ccctggatgc ggagcgtggc gatggctgag ggcctgcggg gcatccgggt
      481 ggcagtgctc accgtcagcg accgggtcgc ggaggggagc cggggcgacg cgggcggcga
      541 cgctctccgc gcgttgctgc aggaggcagg tgcgaccctc acggctcgtg aggtggtgcc
      601 ggacgagcag gcgcggatcg ccgccaccct gcggtcgctc gctgccgccg ccgacgtggt
      661 gctgacaacc ggcggcaccg gcctcggccc tcgcgacgtc acccccgagg cgacccgcga
      721 ggtgctggac cgcgaggcgc ccggcatccc ggaggcgctg cgccacgcca gcctggcgat
      781 cacccccttc gccatgctct cgcgcgccac cgccggcctc gccggccaca ccctgatcgt
      841 caacctcccc ggcaacccga aagcggtcgg cgaggaatgg gaggtcctgg caccgatcct
      901 ggcccacgcc gtcgccaccg cccagggtcc cgtgcccgac gcgagccacc agcatctcaa
      961 gcgataactt ctcggtatcg ctggagatag ctcgttgctc caccttgagc accgtgttac
     1021 gttgccgcga gtttggtcct cctggtcatg cgcgggtcac gcggcttgtt gcgggcttgc
     1081 ggttgtgggc cgggccttcc cccaccagca actaaagaga gggttcgttg tgagcgttcc
     1141 ggctgatcgc caccgccaga tggtggcccg tcgccggcgg gtgctcgtca tcctctcgat
     1201 cgcgtcgctg gctgggctgc gcgcctgggt cgcgctcggc ggccagtggt ggctcgcggc
     1261 ggccatcacc gggtcgctgc tgaccggcta cctggtcgcg ctggcggcgg cgggccgtcg
     1321 cagacacgtc gaagaacccc aagcatccca agccgccgcg ccggccgtcg cgccgccgac
     1381 catcgcggcg agcgttcccg ccgaggcgct gccgctgcct gagccggccg gggaacgggc
     1441 gtcgcggcag cggcggccat tgtcccgccg gctgcgaccc gtggcgaaat gagcttcgcg
     1501 gagatccagc gcggcctcca cgatcggttc gacgccaagc atcgcgcccg tgaggtcggc
     1561 atcggcaacg cccggcaggc gatccgccgc tcggccaacg ccatccgggc ggtccaccgg
     1621 gcggagctcg aggtcgccga ccgcctcatg gtcgaggcgc gggagctgct cgacatcgcc
     1681 gagtcggtgc tggcggccca tcccgacgcc tactacgccg ggttcctgca ggacgcccag
     1741 aaggagtacg ccgag
//
LOCUS       DASPYY010000280         4970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2576466, whole genome shotgun sequence.
ACCESSION   DASPYY010000280 DASPYY010000000
VERSION     DASPYY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576466"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..2347
                     /locus_tag="VEP73_04300"
     CDS             218..2347
                     /locus_tag="VEP73_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25690.1"
                     /translation="MRRQRPERRLALRLAAAALAVGGCLVPAGPAAALVPAGPAAVTR
                     PAQPSGRVVLLLVQRLTWDEAEAAARRAGVPAAAGLVTTLPAGAPLAARVLSLAAGHR
                     VDAGGTDPASVDRFRAANPDGCYLDLPPADVAAAPGLEAARLLAVRATGTPPPPRPLA
                     GRLEPAPGRLLVLAVPGAAGLAGVLRRLPAGTRVLVAGLEPAPGRARTAPFLELGGQA
                     GLATSDSTRRHGLVALEDVCPTLAGPGRARGDGSPIRVVAGRARAAGSPLGATARLDR
                     TAAALVAARTWAIAVLACVSSLALVLLALAWWLAAGTRPTSSPARAARGLVLLTLALP
                     SGYLAASMAAPPSAPQWLGLGVALGVLLAIGAAILGRPSAAGPARPSGVGPGSAAPAW
                     LGAALLALVAGDLLLGGHGLERPLLGGSAFDGERFYGLGNGYFALALASVTVVAAFAP
                     RLGGRAVAGLFAGLAVLDGLPALGADVGGALTAMLTAALAWLLLGAPPAPGSRRGAAG
                     PPRQWGLARALAVLAMALAAGLALAFGVGLVTGEATHGSRFLGRLAAGGPAAAGRIVA
                     RQLGRNVSLLASSVFAWLGPFQVAAAGLLAAWPPAALATALPERVRRVTLVGALGSVV
                     LILSNDTGVTATNAGGLFLLAILAWSLLAWLLWEAGGQASRPAPEGQASRPAPEGQAP
                     AATASRRPPSPPVAARGGASRPPPRGR"
     gene            2436..3875
                     /gene="zwf"
                     /locus_tag="VEP73_04305"
     CDS             2436..3875
                     /gene="zwf"
                     /locus_tag="VEP73_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYT25691.1"
                     /translation="MPGSPVPENHVIVIFGANGDLAGRKLVPGLFHLAADGLMPADYR
                     IVGSSREDLSDDDFRAFARAAVEKAGRVEPDERTWEAFAERLSYVPGEFGPGRTGRLA
                     DAVAAAEARLGDVARRLAYLAIPPTAFAAVTEGLGESGLAAGARVVYEKPFGLGLESF
                     RELNAAVRRVLHESQVYRIDHFLGKESVQNILAIRFANGMYEPVWNRDHIDHVQVDVP
                     EALGIGARAGFYERVGALRDMVVTHLFQVLSFVAMEPPSALSARALTEEKLKVFEAMS
                     PLRPEDLVRGQYEGYRELDGVARDTTTETFVAARVHVDNWRWAGVPFYLRTGKRLAES
                     RSAVTLAFREPPRHLFRSVPADRLETNHLTFSLGPWEGVSISFHAKVPGPALELGPAR
                     MDYRFGSSPGPKTIDAYERLLHDAMCGDRTLFTRGDGVERVWEVVEPVLADPPPPHPY
                     EQGSWGPAAASRLIAPRRWHLPDPDEPGA"
     gene            complement(3886..4155)
                     /locus_tag="VEP73_04310"
     CDS             complement(3886..4155)
                     /locus_tag="VEP73_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25692.1"
                     /translation="MNPGRLAAMNRFEDAIARELSVRLHARTRSVRALARWAAASVPP
                     GALVDDAVRVVTDRLADGDPDALAAAVMAEVPHRFGRNWDSGPAH"
     gene            4235..>4970
                     /locus_tag="VEP73_04315"
     CDS             4235..>4970
                     /locus_tag="VEP73_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT25693.1"
                     /translation="MRAVDLHVHLPLPEWVEGSLGPYREPAERYFGAEIALRRAEELA
                     AAYEAIDCVGVLLGWDAETATGRPPLPNDLVADLVARFPGRFVGFAGIDPWKGTGALR
                     EMRRAHEDLGLAGYKFHPSMQAFRPDDVRWSDLFERAAGYGAPCLFHTGTSGIGAGTP
                     GGQGIELAYARPIHLDAVAARHPELPVVMAHFGWPWHLEAVAIALHKANVHLELSGWR
                     PRYVPDEVVREAEGRLADRVLFGSDAP"
BASE COUNT          469 a         2051 c         1837 g          613 t
ORIGIN      
        1 cggcggggcg ctcggctggt gctcgatcac gaccagggtc gtcaccggcc ctcctcgccg
       61 gcctcgacgc gggcgaccgc cacgccgaag gcggccgtcg tcgctcccat gccgtccatc
      121 ccgcccaggg taaccgctca cggcacgcgg gcgcgatcgg ccggacctcc cgccgcgccg
      181 tcggccggcc cggagccgcg ccggtagaat ccgcccgatg agacgacaac ggccggagcg
      241 ccggctcgcc ctgcgcctcg cggccgcggc cctggccgtg ggcgggtgcc tggtgccggc
      301 agggccggcc gccgccctgg tgccggcagg gccggccgcc gtcacgcgac ccgcccagcc
      361 gtccgggcgg gtcgtgctcc tgctcgtgca gcggctcacc tgggacgagg cggaggccgc
      421 ggcccggcgc gccggggtgc cggcggccgc cgggctcgtc acgaccctgc cggccggcgc
      481 gccgctcgcg gctcgggtcc tgtcgctcgc cgccggccac cgggtcgacg cgggcgggac
      541 cgacccggcc tcggtcgacc gcttccgcgc agccaacccc gacggctgct acctcgacct
      601 gcccccggcc gacgtggccg cggcccccgg cctggaggcg gccaggctcc tcgcggtccg
      661 cgccaccggg accccgccgc cgccccggcc cctggccggc cgcctcgagc cggcccccgg
      721 ccggctgctc gtcctcgcgg tgcccggcgc cgccggcctc gccggcgtgc tccgccggct
      781 gccggccggg acccgcgtgc tcgtcgccgg cctcgagccg gcgcccgggc gggcccgcac
      841 cgcgccgttc ctcgagctgg gcggccaggc gggcctggcc acctcggact cgaccaggcg
      901 ccacggcctg gtcgcgctcg aggacgtctg ccccaccctg gccggcccgg gccgcgcccg
      961 aggcgacggg tcgccgatcc gcgtggtggc cgggcgcgcc cgggcggccg gcagcccgct
     1021 cggcgccacc gcccggctcg atcgcaccgc cgccgcgctc gtcgccgcgc ggacctgggc
     1081 gatcgcggtc ctcgcctgcg tgtccagcct ggcgctcgtg ctcctcgcgc tcgcctggtg
     1141 gctggccgcc ggcacccggc ccacctcgag ccccgccagg gccgcccggg ggctcgtgct
     1201 gctcaccctc gcgctgccct ccggctacct cgccgcgagc atggcggcgc cgccctcggc
     1261 gccgcagtgg ctcgggctcg gcgtggcgct cggcgtcctg ctggccatcg gcgcggccat
     1321 cctcggcagg ccgtccgcgg ccgggccggc caggccgtcc ggggtcgggc cggggagcgc
     1381 cgccccggcg tggctcggcg ccgcgctgct cgcgctcgtg gccggcgacc tcctgctcgg
     1441 ggggcacggg ctcgagcgcc ccctgctcgg cgggagcgcg ttcgacgggg agcgcttcta
     1501 cgggctcggc aacggctact tcgcgctcgc gctggccagc gtcacggtgg tggccgcgtt
     1561 cgccccccgg ctgggcgggc gcgccgtcgc cgggctgttc gccggtctgg ccgtgctcga
     1621 cgggctgccg gcgctcgggg ccgacgtcgg cggcgcgctg accgccatgc tcaccgccgc
     1681 gctggcctgg ctcctgctcg gcgccccgcc ggcccccgga tcccgccggg gcgccgccgg
     1741 cccgccccgc cagtgggggc tcgcccgcgc cctcgccgtc ctcgccatgg ccctggcggc
     1801 cgggctggcg ctcgccttcg gcgtcggcct ggtcacgggc gaggcgaccc acggcagccg
     1861 gttcctgggc cggctcgcgg ccggcgggcc ggccgccgcc ggccggatcg tggcgcgcca
     1921 gctcggacgg aacgtgagcc tcctcgcgag cagcgtcttc gcctggctcg ggccgttcca
     1981 ggtcgcggcc gccggcctcc tcgccgcgtg gccgcccgcg gcgctcgcca ccgccctccc
     2041 cgagcgggtc cgccgcgtga ccctggtcgg cgcgctcggg tcggtggtgc tcatcctctc
     2101 gaacgacacc ggggtcaccg ccacgaacgc gggcggcctg ttcctgctcg cgatcctcgc
     2161 gtggagcctg ctggcctggc tcctctggga ggcgggcggc caggcctcgc ggccggcccc
     2221 ggagggccag gcctcgcggc cggccccgga gggccaggcc cccgccgcga ccgcatcccg
     2281 gaggccgccg tctcccccgg tggcggcccg cggcggggcc tcgcggccac ccccgcgcgg
     2341 gcggtgacat gatgatccca gctgctgcca accccgccgg cgcgccgttg cccaccccgc
     2401 cggcgggtgt ggcgccgctc gagaagggag tccccgtgcc cgggtcgccc gtccccgaga
     2461 accacgtcat cgtcatcttc ggcgccaacg gcgacctggc cggtcgcaag ctcgtgcccg
     2521 ggctgttcca cctcgccgcc gacggcctga tgcccgccga ctaccggatc gtcggcagct
     2581 cccgcgagga cctctccgac gacgacttcc gcgccttcgc ccgcgcggcg gtcgagaagg
     2641 cgggccgggt cgagccggac gagcgcacct gggaggcgtt tgccgagcgc ctgagctacg
     2701 tgcccggcga gttcgggccc gggcgcaccg gccgcctcgc ggacgccgtg gccgccgccg
     2761 aggcccggct cggggacgtg gccaggcgcc tggcctacct cgccatcccg ccgaccgcgt
     2821 tcgcggcggt caccgagggc ctcggcgaga gcggcctcgc cgccggggcg cgggtcgtct
     2881 acgagaagcc gttcggcctc ggcctcgagt cgttccgcga gctgaacgcc gccgtgcgcc
     2941 gggtcctgca cgagtcccag gtctaccgga tcgaccactt cctcggcaag gagtcggtcc
     3001 agaacatcct cgccatccgc ttcgccaacg ggatgtacga gccggtctgg aaccgcgacc
     3061 acatcgacca cgtccaggtc gacgtccccg aggcgctcgg catcggtgcc cgcgccggct
     3121 tctacgagcg ggtcggcgcg ctgcgcgaca tggtcgtcac gcacctgttc caggtgctct
     3181 ccttcgtggc gatggagccg ccgtcggcgc tctcggccag ggcccttacc gaggagaagc
     3241 tcaaggtctt cgaggcgatg agcccgctgc ggcccgagga cctggtccgc ggccagtacg
     3301 agggctaccg ggagctcgac ggcgtggcgc gcgacacgac gaccgagacg ttcgtggccg
     3361 cgcgcgtcca cgtcgacaac tggcgctggg ccggcgtgcc gttctacctc cgcacgggca
     3421 agcggctggc cgagagccgc tcggcggtca ccctcgcctt ccgggagccg ccccgccacc
     3481 tgttccggtc cgtgcccgcc gaccggctcg agacgaacca cctgacgttc tccctcggcc
     3541 cgtgggaggg cgtctcgatc tccttccacg ccaaggtccc ggggccggcc ctcgagctcg
     3601 ggccggcgcg catggactac cgcttcggga gctcgcccgg cccgaagacg atcgacgcct
     3661 acgagcgcct gctccacgac gccatgtgcg gggaccggac cctcttcacc aggggcgacg
     3721 gcgtcgagcg ggtctgggag gtggtcgagc cggtgctcgc cgacccgccg ccgccgcacc
     3781 catacgagca gggctcgtgg ggtcccgcgg cggcgtcccg cctgatcgcc ccgcgccgct
     3841 ggcatcttcc cgacccggac gagcccggcg cctgaccccg cggggtcagt gtgccgggcc
     3901 ggagtcccag ttccggccga accggtgcgg cacctcggcc atgaccgcgg ccgcgagcgc
     3961 gtcggggtcg ccgtccgcga gccggtcggt caccacccgg accgcgtcgt cgaccagcgc
     4021 gccagggggc accgaggccg ccgcccagcg cgccagggcg cgcacgctgc gcgtcctcgc
     4081 atggaggcgg acggacagct cgcgggcgat cgcgtcctcg aaccggttca tggcggccag
     4141 cctacccggg ttcatcggcc gccagcctac gccggccggt gggcgacgcc aggcgcgcgg
     4201 cgtcagcggg cggcccgccg ggtacactcc cggcgtgcgc gccgtcgacc tccacgtcca
     4261 cctgcccctg cccgagtggg tggagggcag cctgggtccg taccgggagc cggccgagcg
     4321 ctacttcggc gccgagatcg cgctgcggcg cgcggaggag ctcgccgccg cctacgaggc
     4381 catcgactgc gtgggcgtgc tgctcggctg ggacgccgag acggccaccg ggcgtccgcc
     4441 cctgccgaac gacctcgtcg ccgacctcgt cgcgcgcttc ccgggccgct tcgtggggtt
     4501 cgccggcatc gatccgtgga agggcaccgg cgcgctgcgg gagatgcgcc gggcccacga
     4561 ggacctcggc ctcgccggct acaagttcca cccctccatg caggcgttcc ggcccgacga
     4621 cgtgcgctgg tccgacctct tcgagcgggc cgccggctac ggcgccccct gcctgttcca
     4681 caccggcacg agcgggatcg gggcgggaac gcccggcggc cagggcatcg agctcgccta
     4741 tgcccggccc atccacctcg acgcggtggc cgcccgccac ccggagctgc ccgtggtcat
     4801 ggcccacttc ggctggccgt ggcacctcga ggcggtggcc atcgcgctcc acaaggccaa
     4861 cgtccacctc gagctgtcgg gctggcggcc gcgctacgtc ccggacgagg tggtccgcga
     4921 ggcggagggc cggctcgccg accgagtgct gttcggctcc gacgcgccgt
//
LOCUS       DASPYY010000281         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_189604, whole genome shotgun sequence.
ACCESSION   DASPYY010000281 DASPYY010000000
VERSION     DASPYY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189604"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VEP73_04320"
     CDS             complement(<1..1921)
                     /locus_tag="VEP73_04320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT25694.1"
                     /translation="MTTVLEPSGAVTPSIRAALRDRDPTPEQWAAIAAPLGPVHVVAG
                     AGSGKTAVMAARIVYLVERLGVPPASVLGLTFTNKAAAELEERVRDALAERRPGLGDE
                     VSVDTYHAFAADLVKAYGVRVGIEPDADLLTEAQQYQILLGILDGERFEHLNVRTAGA
                     TIRKTLELAAACSDHLVPPERVVAESRRLVERVESGERLPEWQAQAARERIELARLVE
                     RYAEEKLRRGRIDFGDQVAKAVELVEGHQDLAEALRARFPHVLLDEYQDTNIAQRRLV
                     QRLCLEGASVMAVGDARQAIYAFRGATMYNLLSFAEHFPRAGRGVEGPLPLSTNFRSG
                     RRILELANAVIEAVPEERSGGRPLVPLAANGEGEVRAALLTDQYAEAAFVADQVAAAH
                     AGGVPGPAGGRTWPAWRDVAVLVRSKRLLGPLREALEQRDVPVEVVGLSGLLETPEIV
                     DLVSMLRVVDDPGANVALARLLLGPRWRLGHRHLVRLARWAASHNRGLKDGLPGEDPD
                     PGDVSFALSEALDHLHEVEGLDDEALARLEAFRTELRGLRAAADTPLLDLVQTVLERT
                     GAWAELAASSDRRAASARQNLATFLDRVAAFAPVEGDPSLPAFLAYLDAVEEAVEPVE
                     ATQPAPADSVKLMTVH"
     gene            complement(1918..>2897)
                     /locus_tag="VEP73_04325"
     CDS             complement(1918..>2897)
                     /locus_tag="VEP73_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYT25695.1"
                     /translation="VLADRNRPAAERLAAAATLAETPGVDPGSWWWRRDWTHDPQPIA
                     AEGRLRTSYSRIGAYEDCHLRYFFGSVAGLDDRTSYQMAFGKLMHTVFELAAKGEVAD
                     GAEALKAAYRERFDPTWFPSRAVAHQFWRDGMEMLRLWHRAEWEAARRALAFEIDFAI
                     EVGPHLVRGRIDRIDRVGDGIALLDYKTGRSPATEEQAASSLQLAIYYIAALRDPELA
                     ALGPPVEMQLVYPARARQGRFTRVSQRPGPDQAERVEQHLLALLDGAAAEAFDPNPHA
                     DCRFCQFKPICPMWPQGEDFLAPAHLPDGLDLAAVATTPGAGRQGDEER"
BASE COUNT          372 a         1071 c         1081 g          373 t
ORIGIN      
        1 tgtgcacggt catcagcttc accgagtcgg ccggggcggg ctgggtcgcc tcgaccggct
       61 cgaccgcctc ctcgaccgcg tcgaggtagg cgaggaacgc gggcagcgag gggtcgccct
      121 cgaccggcgc gaacgcggcc acccggtcga ggaaggtggc caggttctgg cgggccgacg
      181 cagcccgccg gtcgctcgac gccgccagct ccgcccacgc cccggtgcgc tcgaggaccg
      241 tctggaccag gtcgaggagt ggggtgtcgg ccgcggcccg cagcccccgc agctcggtgc
      301 ggaacgcctc gagccgggcg agcgcctcgt cgtccagccc ctcgacctcg tgcaggtggt
      361 cgagggcctc cgacagcgcg aaggacacgt cgcccgggtc ggggtcctcg ccgggcagcc
      421 cgtccttcag cccccggttg tgactcgccg cccagcgggc gagccggacc aggtggcggt
      481 ggccgagccg ccagcgcggc cccagcagga ggcgcgccag ggccacgttc gccccagggt
      541 cgtcgacgac ccgcaacatg ctcaccaggt cgacgatctc cggggtctcg agcaggccgg
      601 acagccccac cacctcgacg ggcacgtccc gctgctccag tgcctcgcgc aacgggccga
      661 gcaggcgctt ggagcgcacc aggaccgcca cgtccctcca ggccggccag gtccggcctc
      721 ccgcggggcc gggaaccccc ccggcatggg cggcggcgac ctggtcggcg acgaaggcgg
      781 cctcggcgta ctggtcggtg agcagggcgg cccgcacctc gccttcgccg ttggcggcga
      841 gcgggacgag cggccgcccg ccgctgcgct cctcgggaac cgcctcgatg acggcgttgg
      901 ccagctcgag gatgcggcgg ccggagcgga agttggtcga gagcggcagc gggccctcga
      961 ccccccggcc ggcgcgcggg aagtgctcgg cgaaggagag caggttgtac atcgtcgcgc
     1021 cgcggaaggc gtagatggcc tggcgggcgt cgccgaccgc catgacggac gcgccctcga
     1081 ggcagagccg ctgcaccagc cggcgctgcg cgatgttcgt gtcctggtac tcgtcgagga
     1141 ggacgtgcgg gaagcgggcc cggagagcct cggcgaggtc ctggtggccc tcgaccagct
     1201 cgaccgcctt ggccacctga tcgccgaagt cgatacggcc ccggcgcagc ttctcctccg
     1261 cgtagcgctc gaccagacgg gccagctcga tgcgctccct ggcagcctgc gcctgccatt
     1321 ccggcaggcg ctcgcccgac tcgacccgct cgacgaggcg cctcgactcg gcgacaaccc
     1381 tctccggggg caccaggtgg tcggagcatg ccgcggcgag ctcgagggtc ttgcggatgg
     1441 tcgccccggc cgtgcgcacg ttcaggtgct cgaagcgctc gccgtccagg atcccgagca
     1501 ggatctggta ctgctgcgcc tcggtcagca ggtcggcgtc cggctcgatg ccgacccgga
     1561 cgccgtacgc cttgaccagg tcggccgcga aggcgtgata ggtgtcgacg ctgacctcgt
     1621 ccccgagccc cgggcggcgc tcggcgagcg cgtcgcgcac gcgctcctcg agctcggcgg
     1681 ccgccttgtt ggtgaacgtg agcccgagca cggaggcggg cgggacgccg agccgctcca
     1741 ccaggtagac gatgcgggcg gccatgaccg cggtcttgcc cgaccccgcg ccggcgacca
     1801 cgtggacggg tccgagcggg gcggcgatcg ccgcccactg ctcgggggtg gggtcgcggt
     1861 cgcgcagggc ggcccggatg ctcggcgtca ccgcgcccga gggctcgagg accgtcgtca
     1921 ccgctcctcg tccccctggc ggccggcccc cggcgtggtc gccacggccg cgaggtccag
     1981 gccgtcgggc aggtgcgcgg gggcgaggaa atcctctccc tgcggccaca tcgggcagat
     2041 cggcttgaac tggcagaacc gacagtcggc gtgcgggttg gggtcgaacg cctcggccgc
     2101 ggcaccgtcc aggagggcga gcaggtgctg ctccacccgc tcggcttggt ccggaccggg
     2161 gcgctggctg acccgcgtga aacggccctg cctggccctt gccggataga cgagctgcat
     2221 ctcgacgggc gggccgaggg cggcgagctc ggggtcccgg agggcggcga tgtagtagat
     2281 cgcgagctgg agcgagctcg cggcctgctc ctcggtggcc gggctccgcc ccgtcttgta
     2341 gtcgaggagg gcgatcccgt cacccactcg gtcgatcctg tcgatgcgtc ctcggacgag
     2401 gtgcgggccc acctcgatgg cgaagtcgat ctcgaacgcg agcgcccgcc tggccgcctc
     2461 ccactccgcg cgatgccaca gcctgagcat ctccatcccg tcccgccaga actggtgcgc
     2521 gaccgcccgg gaagggaacc acgtagggtc gaaccgttcc cggtaggccg ccttgagggc
     2581 ctccgccccg tcggcgacct cccccttggc ggccagctcg aacaccgtgt gcatcagctt
     2641 gccgaacgcc atctggtagg aggtccggtc gtcgaggccg gcgacgctgc cgaagaagta
     2701 gcgcaggtgg cagtcctcgt acgcgccgat ccgggaatag gaggtgcgca gcctgccctc
     2761 ggccgcgatc ggctggggat cgtgggtcca gtcgcgccgc caccaccagg acccggggtc
     2821 caccccgggc gtctccgcga gcgtggccgc cgcggccagc cgctcggccg ccgggcggtt
     2881 gcggtccgcg agcaccc
//
LOCUS       DASPYY010000282         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1876275, whole genome shotgun sequence.
ACCESSION   DASPYY010000282 DASPYY010000000
VERSION     DASPYY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876275"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEP73_04330"
     CDS             <1..510
                     /locus_tag="VEP73_04330"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYT25696.1"
                     /translation="RTARDNDWAMLGPTRIEFETDEQLPEGRFRVTGRVEADSSRAEP
                     APEADGGDAPVGPYTAMLPGQSRAPKPRTTASLVLLSGRQEVQVYPLAGTEHSIGRSE
                     GSDIALADPGVSRNHARIVREGDDFIVEDLQSTNGTEVNGRPVRRRRLADGDLVKLAN
                     STLQFRRES"
     gene            510..977
                     /locus_tag="VEP73_04335"
     CDS             510..977
                     /locus_tag="VEP73_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYT25697.1"
                     /translation="MPPIFLLAVKVAFLAILYLFVARAVRAVVLDVFGPRAQRRRPAR
                     GAPRPAARAAPARPSRRPARELVVADSSGKRTVPLKDSITVGRAATCDLVLSDTYVSN
                     VHARIFAKDGAYWLEDLGSTNGTYMNRAKVAVPTAIGPGDEIRMGKATLELRR"
     gene            977..2266
                     /locus_tag="VEP73_04340"
     CDS             977..2266
                     /locus_tag="VEP73_04340"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HYT25698.1"
                     /translation="MTLRRGAATDRGRMREGNEDSYLAAGPLAAVADGMGGHRAGEVA
                     SATAMEQLRFLRDGGPWLDDRAAGEALKRAVAEANRRIRDLAATDKALEGMGTTITAL
                     LEDRDSIHLAHVGDSRAYLLRHGELSSLTEDHTLVQELVRQGRLRPEDAERHPQRSII
                     TRALGVDADVEIDTATYKIVPGDRLLLCTDGLSGVVDQARIRNVLLRVPDPQQAAEKL
                     VAMANEAGGPDNITVIVLDADGARSDTADPTGDLAADEPGSGDGPVAGTDDALVTGLR
                     RVGPGAGGRARVQPGRAGRSRPRRRRARRVLTALLVLAALAGITLAGRAFLYSRYWVG
                     FHGDRVAVFRGVPGEVAGLHFSWLVRDSGVTRAQVPAGYTSNLEDGVTAKSRADALRI
                     ANCVPNVYKGCPAGTGPVTGTTVPATTRAAPTTAPKG"
     gene            2266..3633
                     /locus_tag="VEP73_04345"
     CDS             2266..3633
                     /locus_tag="VEP73_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYT25699.1"
                     /translation="MVESRRNVELTLLLLALVLSVGGYIVVGLVADNKVPAGSVGYGA
                     TLALLFIGAHLVLRWKAPQADPILLPGAALLNGLGLVMVRRLDYAAVLRRGGHPQAPS
                     QALWTVLGVLVFAGVVILVRDHRVLDRYRYTWLLIGLFLLVLPRLPGIGQEINGARLW
                     VRIGPFSGQPAELAKIALVVFLASYLAERKELLATATYRVGPLLLPEIKYFLPMLIAW
                     GVSLFVLFFEKDLGSSLLFFGVFVALLYVATGRFSYVTAGLGLFAVGAFIAYHLFAHV
                     QVRFATWLHVWDYYNGRGYQLAQGLFALATGGIFGQGWNQGQPNLIPFASTDFVFDAF
                     GEELGLLGVTALLLVFLLMTFRGFRVALAASDDFGKLLGLGLVVSFALQVFVIVGGVT
                     RLIPLTGITLPFVSYGGSSLVANYALVAILCRVSSDATPAPARRAGAGPADAGAESGE
                     LTVVR"
     gene            3630..>4147
                     /locus_tag="VEP73_04350"
     CDS             3630..>4147
                     /locus_tag="VEP73_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYT25700.1"
                     /translation="MNRQVRRVGIVVVVCFLALFVQLNWLQVIDAHSLATNPRNVRAV
                     LRDYSQPRGQILTSDGVVVARSDPVNDEFERLRTYPTGPLFAHTSGFFSFTLGASAVE
                     DAYNRDLSGRNVDRQFHKLGDWLIGKEQTGDAVLSLSNVAQEAARDALGDRKGSVVVL
                     DPATGEVVALWS"
BASE COUNT          532 a         1558 c         1462 g          595 t
ORIGIN      
        1 cggaccgcgc gggacaacga ctgggccatg ctgggcccga cccggatcga gttcgagacc
       61 gacgagcagc tgcccgaggg ccgcttccgg gtgaccggac gggtcgaggc cgactcgagc
      121 cgggccgagc cggcgccgga ggccgacggc ggcgacgcgc cggtggggcc gtacaccgcc
      181 atgctcccgg ggcagtcccg tgcccccaag ccccggacca cggcgagcct cgtgctcctg
      241 tccggccgcc aggaggtgca ggtctacccg ctggccggca ccgagcactc gatcggccgc
      301 tccgaagggt cggacatcgc acttgccgat cccggcgtct cgcgcaacca tgcgcggatc
      361 gtccgcgagg gcgatgactt catcgtcgag gacctgcagt ccaccaacgg gaccgaggtg
      421 aacggccgac ccgtgcggcg gcgccgtctg gccgacgggg acctggtgaa gctggcaaac
      481 tcgacgctgc agttccgacg ggagagctga tgccgccgat cttcctgctc gcggtcaagg
      541 tcgccttcct ggccatcctg tacctgttcg tggccagggc ggtccgcgcc gtcgtgctcg
      601 acgtgttcgg gccgcgggcg cagcgccgcc ggccggccag gggggcgccc cgccccgccg
      661 cccgggcggc gcccgcccgt ccctcccgcc gcccggcccg cgagctggtc gtcgccgact
      721 cgagcggcaa gcgcaccgta ccgctgaagg actccatcac ggtcggccgg gccgcgacct
      781 gcgacctcgt cctcagcgac acgtacgtgt ccaacgtgca cgcgcggatc ttcgccaagg
      841 acggcgcgta ctggctcgag gacctgggct ccacgaacgg cacgtacatg aaccgcgcca
      901 aggtggccgt gccgacggcc atcgggcccg gggacgagat ccgcatgggg aaggcgacct
      961 tggagctgcg ccgctgatga ccctccgacg tggagccgcc accgaccggg gtcggatgcg
     1021 cgagggcaac gaggacagct acctggctgc cggcccgctc gccgcggtcg ccgacggcat
     1081 gggcggccac cgggccggcg aggtcgccag cgcgaccgcc atggagcagc tgcgcttcct
     1141 gcgcgacggc ggcccctggc tcgacgaccg ggccgccggc gaagccctga agagggcggt
     1201 ggccgaggcc aaccggcgga tcagggacct ggcagcgacc gacaaggcgc tcgagggcat
     1261 gggcaccacc atcacggccc tcctcgagga ccgggactcg atccacctcg cccacgtcgg
     1321 cgactcgcgc gcctacctgc tgcgccacgg cgagctgtcg agcctcaccg aggatcacac
     1381 gctcgtgcag gagctggttc gccagggccg tctgcgcccc gaggacgccg agcgccaccc
     1441 ccagcgcagc atcatcaccc gggccctcgg tgtggacgcg gacgtcgaga tcgacaccgc
     1501 cacctacaag atcgtcccgg gggaccgcct cctgctctgc accgacggcc tgtcaggcgt
     1561 cgtcgaccag gcgcggatcc gcaacgtcct gctgcgcgtc cccgaccccc agcaggccgc
     1621 cgagaagctg gtcgccatgg cgaacgaggc cggcggcccg gacaatatca cggtgatcgt
     1681 gctggacgcc gacggggccc ggagcgacac ggcggacccg accggcgatc tcgccgcgga
     1741 cgagccgggc tccggggacg ggccggtcgc cggcaccgac gacgcgctgg tgaccggcct
     1801 gcggcgggtc ggcccgggcg ccgggggaag ggcccgggtg cagccgggcc gcgccgggcg
     1861 ctcgcgcccc cgccggcgca gggccaggcg cgtgctcacg gccctgctcg tgctcgccgc
     1921 cctcgccggg atcaccctgg ccgggcgtgc cttcctctac agccgctact gggtcgggtt
     1981 ccacggcgac cgggtggcgg tgttccgcgg cgtccccggc gaggtggccg gcctgcactt
     2041 ctcctggctg gtgcgggaca gcggcgtgac ccgcgcccag gtgcccgccg gctacacgtc
     2101 caacctggag gacggcgtca ccgcaaagag ccgcgccgac gccctgcgga tcgccaactg
     2161 cgtcccgaac gtgtacaagg gctgcccggc cggcaccggg ccggtgaccg gcaccaccgt
     2221 cccggccacg acgagggccg cgccgaccac ggcgccgaag ggctgatggt ggagtcgcgc
     2281 cgcaacgtcg agctgacgct gctcctcctg gccctggtgc tgtcggtggg cggctacatc
     2341 gtcgtcggcc tggtggccga caacaaggtc ccggccggca gtgtcggcta cggcgcgacc
     2401 ctggccctgc tgttcatcgg cgcgcacctg gtcctgcgct ggaaggcgcc ccaggccgac
     2461 ccgatcctgc tgcccggggc cgccctgctc aacgggctcg gcctcgtcat ggtccgccgc
     2521 ctcgactacg cggccgtgct gcgcaggggc ggccacccgc aggcgccctc ccaggccctg
     2581 tggacggtgc tcggcgtcct ggtcttcgcc ggggtggtga tcctggtccg cgaccaccgg
     2641 gtgctcgacc gctaccgcta cacctggctg ctcatcggcc tcttcctgct ggtgctgccc
     2701 cggctgccgg gcatcggcca ggagatcaac ggcgcccggc tctgggtgcg gatcgggccg
     2761 ttctcgggcc agcccgccga gctggccaag atcgcgctgg tggtgttcct cgcgtcctac
     2821 ctggccgagc gcaaggagct cctggccacg gcgacctacc gcgtcggccc gctgctcctg
     2881 cccgagatca agtacttcct gcccatgctg atcgcctggg gcgtcagcct cttcgtgctg
     2941 ttcttcgaga aggacctggg ctcgagcctg ctgttcttcg gggtgttcgt tgccctgctc
     3001 tacgtcgcga ccgggcgctt ctcgtacgtc acggccggcc tcggcctgtt cgcggtgggc
     3061 gccttcatcg cctaccacct cttcgcccac gtacaggtcc ggttcgcgac ctggctgcac
     3121 gtctgggact actacaacgg ccgcgggtac cagctggccc aggggctgtt cgcgctggcc
     3181 accgggggga tcttcgggca gggctggaac cagggacagc ccaacctgat cccgttcgcc
     3241 tcgaccgact tcgtgttcga cgccttcggg gaggagctcg gcctcctcgg cgtcaccgcc
     3301 ctgctgctcg tcttcctgct gatgacgttc cgcggcttcc gggtcgccct cgccgcgagc
     3361 gacgacttcg gcaagctgct cggcctcggt ctcgtggtca gcttcgcgct gcaggtcttc
     3421 gtgatcgtcg gcggggtgac caggctcatc ccgctgaccg gcatcacgct gcccttcgtc
     3481 agctacggcg ggtcgagcct ggtggccaac tacgctctcg tcgccatcct ctgccgcgtc
     3541 tcgagcgacg cgaccccggc gcccgcccgc cgggctggcg ccgggccggc cgacgccggc
     3601 gccgagagcg gggaactgac ggtggtccgg tgaaccgcca ggtccgccgg gtggggatcg
     3661 tcgtcgtcgt ctgcttcctg gccctgttcg tgcagctcaa ctggctgcag gtcatcgacg
     3721 cccacagcct ggccaccaac ccccgcaacg tgcgggcggt gctgcgcgac tactcgcagc
     3781 cccggggtca gatcctgacc agcgacgggg tcgtggtggc ccgctccgac ccggtcaacg
     3841 acgagttcga gcgcctccgc acctatccca ccgggccgct gttcgcccac acgagcgggt
     3901 tcttctcctt cacgctgggg gcgtcggccg tcgaagacgc ctacaaccgc gacctgagcg
     3961 gccggaacgt ggaccggcag ttccacaagc tcggtgactg gctgatcggc aaggaacaga
     4021 ccggggatgc ggtcctttcg ctcagcaacg tcgcccagga agcggcccgc gacgccctcg
     4081 gcgaccggaa ggggtcggtg gtggtgctcg acccggccac cggcgaggtg gtggcgctgt
     4141 ggtcgta
//
LOCUS       DASPYY010000283         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_611083, whole genome shotgun sequence.
ACCESSION   DASPYY010000283 DASPYY010000000
VERSION     DASPYY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611083"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VEP73_04355"
     CDS             complement(<1..972)
                     /locus_tag="VEP73_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25701.1"
                     /translation="MAIRHVATRPAGRGRRRAVLALLCVAQFVDVLGVTVVLVALPSI
                     QRDLGFSAAGLQWVASGYALAFGGLLMLSGRAADLYGRRRLFMAGLALFALASLACGL
                     AGAREVLVVARAAQGAGAALVVPAALSLLTTTFTDGPAPDGLTRTRALGIWTAAAAGG
                     GAAGFAVGGLLTGGLRWRWVFLVNVPIGAAGLALAPGLLTGRSERRARRGGRGLDVPG
                     ALAVTAGLVLAIYGFTHAEHAGFGAAGTVGALALAGALLASFPAIERRAADPLVPPGT
                     FRTRGLAGATLVAFALTAVTSPAGVLAALHLQEVLGYSPTATGLALLP"
     gene            1079..2173
                     /locus_tag="VEP73_04360"
     CDS             1079..2173
                     /locus_tag="VEP73_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT25702.1"
                     /translation="MATLKDVAAVAGVSRTTVSNAYNRPGKLSGAVRDRVLAAARDLG
                     YPGPDPTARSLRTGRVGALGVLLAEALGFAFDDPAAVELLRGIAETGELAGVGLTLLP
                     APPEQPVDASAVARAVVDGFLVYHLPDDHPSLRAVVARGLPTVVVDGPDLEGVPLVGV
                     DDLGGARQAAEHLLALGHRRIGVLVDRIAADAHRGPVGPERRGTGGFRAARQRLAGYA
                     DALRAAGLRWDAVPIEECGVPNAASGRAGAAALLDRAPGCTALLATTDLLALGAVDAA
                     RERGLPVPGALSVAGFDDLPAAAAAGLTTVRQPLADKGRVAARLLLEVLGGAVPAARV
                     ELPTRLVARGSTAPPGARSPRTVLQPRTLH"
     gene            2291..>2495
                     /locus_tag="VEP73_04365"
     CDS             2291..>2495
                     /locus_tag="VEP73_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25703.1"
                     /translation="MEQTPAARQGRGPSSSPPGGWSLRVQSVLYANDPAGIARFLAGL
                     ARAAEHAKQAKAAHMVTCWLGDCS"
BASE COUNT          304 a          953 c          967 g          271 t
ORIGIN      
        1 gggcagcagc gccaggccgg tcgcggtggg ggagtacccg agcacctctt ggaggtggag
       61 ggcggcgagc accccggcgg ggctggtcac ggcggtcagt gcgaacgcca cgagggtggc
      121 gccggccagc ccccgcgtgc ggaacgtgcc tggcgggacc agggggtcgg ccgcccggcg
      181 ctcgatcgcc gggaagctcg cgaggagggc gccggcgagg gcgagcgccc cgacggtgcc
      241 ggccgccccg aacccggcgt gctcggcgtg cgtgaatccg tagatggcga ggacgagccc
      301 ggcggtgacg gccagcgcgc ccggcacgtc gagcccgcgt cctccccgcc gggcgcgccg
      361 ctcactgcgc ccggtgagca gcccgggggc cagtgccagg ccggccgcgc cgatcggcac
      421 gttcacgagg aacacccacc gccagcggag cccgccggtg agcagcccgc cgacggcgaa
      481 gccggccgcg cccccgccgg ccgcggccgc ggtccagatc ccgagggcgc gggtgcgtgt
      541 cagcccgtca ggggccgggc cgtcggtgaa cgtggtggtg agcagcgaca gcgccgccgg
      601 gacgaccagc gccgccccgg cgccctgagc ggcgcgcgcg acgacgagga cctcccgggc
      661 gccggccagc ccgcatgcca gcgaggccag cgcgaacagg gcgagcccgg ccatgaacag
      721 ccggcggcgc ccgtagaggt cagccgcccg ccccgacagc atcagcagtc ccccgaaggc
      781 cagcgcgtag ccgctcgcca cccattgcag gccggccgcc gagaagccga ggtcgcgctg
      841 gatcgagggc agggcgacga ggacgaccgt gacgccgagg acgtccacga actgcgccac
      901 gcagagcagg gcgagcaccg cccggcggcg gccgcgcccg gccggcctgg tcgccacgtg
      961 cctgatcgcc acctgctttg ttgatcgatt caccatccgg agtctaggct gaggtgaatc
     1021 gatcaacaag gtgaatcgat caacaagggg atcgagcgcc gtgcggcagg gagggcgagt
     1081 ggccacgctg aaggacgtcg ccgcggtcgc gggcgtgtcc cgcacgacgg tctccaatgc
     1141 ctacaaccgg cccgggaaac tctccggggc cgtgcgcgac cgcgtcctcg ccgccgcaag
     1201 ggacctcggc tacccgggcc ccgacccgac cgcccgcagc ctgcgcaccg gccgggtcgg
     1261 cgcgctcggc gtgctgctgg ccgaggcgct ggggttcgcg ttcgacgacc ccgcggcggt
     1321 cgagctgctg cgcgggatcg ccgagaccgg ggagctggcg ggggtgggac tgaccctgct
     1381 gccggcgccg cccgagcagc cggtcgacgc gtccgccgtc gcccgggcgg tcgtggacgg
     1441 cttcctcgtc taccacctgc ccgacgacca ccccagcctg cgcgcggtcg tggcgcgcgg
     1501 cctccccacc gtggtcgtgg acggccccga cctcgagggc gtcccgctcg tcggggtgga
     1561 cgacctgggc ggcgcccgcc aggccgccga gcatctcctg gccctcggcc accgccggat
     1621 cggggtgctc gtcgaccgga tcgcggcgga cgcccaccgc ggtccggtcg gcccggagcg
     1681 gcgcgggacg ggcgggttcc gggccgccag gcagcggctg gccggctacg ccgacgcgct
     1741 gcgggcggcc ggcctccgct gggacgcggt gccgatcgag gagtgcggcg tcccgaacgc
     1801 ggccagcggg cgcgcgggcg cggcggcgct cctggaccgg gcgcccggat gcaccgcgct
     1861 gctggcaacc acggacctgc tcgccctcgg cgcggtcgac gccgcgcgcg agcgcggcct
     1921 gccggtcccg ggcgccctgt cggtcgccgg cttcgacgac ctgcccgcgg ccgccgccgc
     1981 gggcctcacc accgtgcgcc agcccctcgc cgacaagggc agggtcgcgg cccgcctgct
     2041 cctcgaggtg ctgggcggcg cggtccccgc cgcgcgggtc gagctgccca cccggctggt
     2101 cgcgcgcggc tcgaccgccc cgccaggagc tcggtcacct cggacggtgc tccagccgcg
     2161 tacgctccac tgagctggct cacctcaggg gccatgcggg actgataggt atggttgcgc
     2221 ccttggtggc tgtttgcgta gtgcccaccg gccagggtct gaccgctcga cggcgatgat
     2281 gaggagctgt atggagcaga cgccggcggc ccggcagggg cgcggcccgt cgagttcccc
     2341 acctggcggt tggtccctgc gtgtccagag cgtcctgtac gccaacgacc cggcgggcat
     2401 cgcgcgcttt ctggccggcc tcgcccgggc cgccgagcac gccaagcagg ccaaggccgc
     2461 gcacatggtg acgtgctggc tgggtgactg ctccc
//
LOCUS       DASPYY010000284         5486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2016376, whole genome shotgun sequence.
ACCESSION   DASPYY010000284 DASPYY010000000
VERSION     DASPYY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016376"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VEP73_04370"
     CDS             complement(<1..358)
                     /locus_tag="VEP73_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HYT25704.1"
                     /translation="MAISQVSGGEVAYPDGRHALAEGVDLSDSPLYNPDLAPVPIERR
                     NWTTYNYAALWVGMAHNIPSYLLASGLILLGMDWVQALMTIALGNLLVLVPMLLNSHA
                     GTKYGIPFPVFARASFG"
     gene            complement(508..1911)
                     /locus_tag="VEP73_04375"
     CDS             complement(508..1911)
                     /locus_tag="VEP73_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HYT25705.1"
                     /translation="MDAHVLASTLRPRLADVEEGRFGRLFPELDPEPSDERALLALGA
                     AGGPLEAVSALPGEADNPRIAAGWAFFGQFVAHDVTHDRAPLQRHEDTATLRNYRSPR
                     LDLECVYGAGPVGQPYLYDLDDGDKLLVPSSDDVPRNHQGRGLVADARNDTHLFVSQL
                     HLALLRFHNRIVDHVRARGVQGELVLAEAQRLARWHYQWIVTNEYLPLSVGDELLADL
                     EANGPRVCRFGGRPFIPVEFSDGAFRFGHSQVRSSYAVNAGARDVPLFPELVGSRPVT
                     PEWVVDWTELFACPGRPAPQPSRRIGARLAGPLLRLPERLVGDTEPSLAARDLERGRS
                     VDLPSGEAVAHALGLTPCTHGELGLEGVTWPGETPFWLYVLLEAERQEDGLRLGEVGG
                     RIVAEVLLGLLDADPGSYRRAAPGWRPELPGADPGRFTMADLLAFAGLLPAPQLGGAP
                     RRSTFSARHLHGLPPGN"
     gene            2007..2786
                     /locus_tag="VEP73_04380"
     CDS             2007..2786
                     /locus_tag="VEP73_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT25706.1"
                     /translation="MTTDAELDTSPAGLIDRLGRLMAYLQPELEAVFAEFGLTRAGFD
                     VLATLRRSGPPYRLPQRALQDSLLRTSGTVSIRVDALEAAGLVARQPDPADRRGVLVA
                     LTQRGRELADAVAPAHLANEELLLSGLTVEERAQLAGLLGKLLQSFEPAGGEEPAGPG
                     GRRLGMAVAPPHVARRLRRAVGLPDRIGLLVRAVEPGGPAARAGLAQGDLLVAAAGRQ
                     LRSVAGLRQALEAGAGRLELTVVRGTDERTVPVAPGPAGAG"
     gene            2974..4098
                     /locus_tag="VEP73_04385"
     CDS             2974..4098
                     /locus_tag="VEP73_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25707.1"
                     /translation="MTIPRRIGWVWRTAPEPPPAGLEAMLVRAANGTSTKGSGGFDYA
                     VNYRAWKRALGGRALPWTWLGPPASTDGIAAAEAVAQAAPGEPLYVVDLEGSVPDTEV
                     VAFANRLRALVPGSLVGFSSYPTRAQATSHGVPWDACIAAFDLGLPQVYFPRQRAALA
                     QVVADHRGRGVHVAVSPGDDPGWLATAEAGVGTHLGASLWRHGLDQFTAWAQELGTVH
                     GGDEFDMATIADLENAVRKVLNEGAGQGQPSWATTSAAGFAATQSLPDRLDRVEQVTQ
                     ATREAVDALHLTLTGPEVQAIADAVGARLADLPPATLTEGEVQAIADAVAAKLMDIPL
                     GTVALDDASVTRVGEEVSGLVTDRLGPGVIAALRTQFQKP"
     gene            complement(4158..4646)
                     /locus_tag="VEP73_04390"
     CDS             complement(4158..4646)
                     /locus_tag="VEP73_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25708.1"
                     /translation="MPPDTTGKGSDLETVYHRYFSSLGTSAGPRSLRTAKTLQRLRRQ
                     AVVAVLGALALGVFLGVAFRGTQAGAGTRTVTRVVPSPACGRAVARANRSLGYAVQVE
                     KALAEHTEYMNELMNGKITPSQALQQGMPSLVMGASASARFDSALADYHQVVRSCQLP
                     SR"
     gene            complement(4853..>5486)
                     /locus_tag="VEP73_04395"
     CDS             complement(4853..>5486)
                     /locus_tag="VEP73_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HYT25709.1"
                     /translation="HRAELTGEAIRLAREVRRLLPDDGEVTGLLALMLLTDARRPART
                     RPDGSLVPLAEQDRGRWDQEAIREGVALITDTLTRAPLGPYQLQAAIAAVHDEAARAR
                     DTDWPQILALYQLLERIAPNPMVTLNHAVAVAMVHGPQAGLDLLDSLDADERLAGHHR
                     LDAVRAHLLEMAGDHEAARACYRQAARRTTSLPERRYLETRAAQLTEDDQ"
BASE COUNT          665 a         2050 c         2075 g          696 t
ORIGIN      
        1 ccccgaacga cgcgcgggcg aacaccggga aggggatgcc gtacttcgtc cccgcgtggc
       61 tgttcagcag catcgggacc agcacgagca ggttgccgag cgcgatcgtc atgagcgcct
      121 gcacccagtc catgccgagc aggatcaggc cggaggcgag caggtaggag gggatgttgt
      181 gggccatccc gacccacagg gccgcgtagt tgtaggtggt ccagttccgc cgctcgatcg
      241 ggaccggggc gaggtcgggg ttgtagaggg ggctgtcgga gaggtcgacg ccctcggcga
      301 gcgcgtgccg gccgtcgggg taggcgacct cgccaccgga cacttgggat atggccatcg
      361 caggtcctca ccttcgcaac cacatgccac cgcaaccaca tgccgcaacc tcgtcccgct
      421 cgccggccgc gcgcggggcg tccctggccg ccgtcccgat caccccgctc tggcggtgcg
      481 ccggtgggga gcccggtcgc gagcatgcta gttcccgggg ggaaggccgt gaaggtgacg
      541 ggccgaaaat gttgaccgcc gcggcgcgcc gcccagctgc ggggcgggga ggaggccggc
      601 gaaggccagc aggtcggcca tggtgaaccg gcccgggtcg gcgcccggca gctcggggcg
      661 ccagccaggg gccgcgcggc ggtaggagcc gggatcggcg tcgaggaggc cgagcagcac
      721 ctcggccacg atccgcccgc cgacctcgcc gagccgcagc ccgtcctcct ggcgctcggc
      781 ctcgaggagg acgtagagcc agaacggcgt ctcgccgggc cacgtgaccc cctccaggcc
      841 gagctcgccg tgggtgcacg gcgtcagccc gagcgcgtgg gcgaccgcct cccctgaggg
      901 caggtcgacc gagcggcccc gttcgaggtc cctggccgcg agcgacggct cggtgtcgcc
      961 caccagccgc tccgggagcc tgagcagcgg gccggcgagc cgggcgccga tcctgcggct
     1021 cggctgcggc gccggccgtc ccgggcacgc gaacagctcc gtccagtcca ccacccactc
     1081 cggggtgacg gggcgggacc cgacgagctc ggggaacagc ggcacgtcgc gcgcgcccgc
     1141 gttgaccgcg tagctcgacc ggacctggct gtggccgaag cggaacgccc cgtcgctgaa
     1201 ctcgaccggg atgaacggac ggccgccgaa gcggcacacc cgcggcccgt tggcctcgag
     1261 gtcggcgagc agctcgtcgc cgacgctgag cggcaggtac tcgttcgtga cgatccactg
     1321 gtagtgccag cgggcgaggc gctgggcctc ggcgagcacc agctccccct gcacgccccg
     1381 cgcccgcacg tggtcgacga tccggttgtg gaagcgcagg agggcgaggt ggagctggga
     1441 gacgaacagg tgggtgtcgt tgcgggcgtc ggccacgagc cccctgccct ggtggttgcg
     1501 cggcacgtcg tcgctcgacg ggaccagcag cttgtcgccg tcgtcgaggt cgtacaggta
     1561 gggctggccc accggcccgg caccgtagac gcactcgagg tcgagcctgg ggctgcggta
     1621 gttccgcagc gtcgcggtgt cctcgtgccg ctgcaggggg gcgcggtcgt gggtgacgtc
     1681 gtgggcgacg aactggccga agaaggccca gccggcggcg atgcgaggat tgtccgcctc
     1741 gccgggcagc gccgacacgg cctcgagcgg ccccccggcg gcgccgagcg cgagcagcgc
     1801 ccgctcgtcg gacggctcgg ggtcgagctc ggggaacaac cggccgaacc tgccctcctc
     1861 gacgtcggcg agccgcggcc ggagcgtgct ggcgagcacg tgcgcgtcca tgttggcccc
     1921 ttcgttcgtc ggcccgttcg ccgggcccct ctctatgccg ggccccctct ctctcaagat
     1981 gcttgagctc aaggtaccat ccgcgcatga cgaccgacgc cgagctcgac acctcgccgg
     2041 ccggcctgat cgaccgcctc ggacgcctca tggcctatct gcaacccgag ctggaggcgg
     2101 tcttcgccga gttcgggctc acccgggcgg ggttcgacgt gctcgccacg ctgcgcaggt
     2161 ccggtccgcc gtaccgcctg ccgcagcgcg ccctgcagga ctcgctgctg cggacgtcgg
     2221 gcacggtcag catccgggtc gacgcgctcg aggcggccgg cctggtcgcc cgccagccgg
     2281 accccgccga ccggcgcggg gtgctcgtgg ccctcaccca gcgagggcgg gagctggccg
     2341 acgccgtcgc gcccgcccac ctcgccaacg aggagctgct cctgtccggg ctcaccgtcg
     2401 aggagcgcgc gcagctcgcc ggcctgctcg gcaagctgct gcagagcttc gagcccgccg
     2461 ggggcgagga gcccgccggc ccgggcggcc gccgcctcgg catggccgtc gcccccccgc
     2521 acgtggcacg ccggctgcgc cgcgcggtcg gcctgccgga ccgcatcggg ctcctggtcc
     2581 gcgccgtcga gccgggcggc ccggcggccc gggccggcct ggcccagggg gacctcctcg
     2641 tggctgccgc cggccggcag ctccgctccg tggccggcct caggcaggcg ctcgaggccg
     2701 gggccgggcg gctcgagctg accgtcgtgc gcgggaccga cgagcggacc gtgccggtcg
     2761 cgcccggccc tgccggggcg ggatgacggg ggcggcgggg atgaccgggc ggcccccggg
     2821 ccgcccgtgt gcggtcccag cccttgacct gctgcggccc gggcacgcag gcaggcgccc
     2881 gccggccagg gcgcggtagg ctccgcccga caaggacacg gccgtggcct gctcgcgcgc
     2941 ggggccagcg gtgcccggga ggtagcgccg ccagtgacga ttccccgacg catcggctgg
     3001 gtctggcgca cggcgcccga gccgccgccg gccgggctcg aggcgatgct cgtccgcgcg
     3061 gccaacggca cgtcgacgaa gggctcgggc gggttcgact acgcggtcaa ctaccgcgcc
     3121 tggaagcgcg cgctcggcgg ccgggcgctg ccgtggacgt ggctcgggcc gccggcgagc
     3181 accgacggca tcgccgcggc cgaggcggtc gcccaggccg ctcccggcga gcccctctac
     3241 gtggtcgacc tcgagggcag cgtgcccgac accgaggtgg tcgcgttcgc gaaccggctg
     3301 cgcgccctcg tccccgggtc cctcgtcggg ttctcgagct accccacgag ggcgcaggcc
     3361 acgagccacg gcgtcccgtg ggacgcgtgc atcgccgcct tcgacctcgg cctgccccag
     3421 gtctacttcc cgcggcagcg cgccgcgctc gcccaggtcg tcgccgacca ccgcggccgt
     3481 ggcgtccacg tggccgtctc gccgggcgac gacccgggct ggctcgcgac cgccgaggcc
     3541 ggcgtgggca cgcatctcgg cgcttcgctc tggcgccacg ggctcgacca gttcaccgcc
     3601 tgggcgcagg agctcgggac cgtacacgga ggagacgagt tcgacatggc gaccatcgcc
     3661 gacctcgaga acgccgtccg caaggtgctg aacgagggcg ccggccaggg ccagccgtcc
     3721 tgggccacga ccagcgccgc gggcttcgcg gccacccagt ccctgcccga ccggctcgac
     3781 cgggtggagc aggtcaccca ggcgaccagg gaggccgtcg acgcgctaca cctcaccctg
     3841 accggcccgg aggtgcaggc gatcgccgac gcggtcggcg ccaggctcgc ggacctgccg
     3901 ccggccacgc tgaccgaggg cgaggtgcag gcgatcgcgg acgcggtcgc ggccaagctc
     3961 atggacatcc cgctcggcac cgtcgccctc gacgacgcga gcgtcaccag ggtgggggag
     4021 gaggtctcgg gccttgtgac cgaccgcctc ggccccggcg tgatcgccgc cctgcgcacg
     4081 cagttccaga agccataggc tcggggttcc gcggccaccc ggtccctgcc cggggtgcac
     4141 ccgggtccgg cggcggctca gcgggacggc agctggcagc tcctgaccac ctggtggtag
     4201 tcggccagcg ccgagtcgaa gcgcgccgac gcgctcgcgc ccatgacgag cgacggcatc
     4261 ccctgctgca gcgcctggct cggcgtgatc ttcccgttca tcagctcgtt catgtactcg
     4321 gtgtgctcgg cgagcgcctt ctcaacctgg accgcgtagc cgaggctccg gttcgcccgg
     4381 gcgaccgccc tgccgcacgc cgggctgggc acgacccggg tcacggtccg cgtcccggca
     4441 cccgcctggg tgccacggaa cgccacgccg aggaagacgc cgagcgccag ggcgccgagc
     4501 acggcgacca cggcctgcct gcggagccgc tggagcgtct tggccgtcct gagcgacctg
     4561 gggccggcgg acgtccccag cgaggagaag tagcggtggt acaccgtctc gaggtcgctt
     4621 cccttgccgg tcgtgtccgg aggcatccgt ccctctcaag ctcgtcacgc cccgtccggt
     4681 cgcgtgggcg gccagcccgc ggcgggctcc tgtgcccctg gaaccccggc gggcgcgtgt
     4741 gcgcgtcctt gccacagcaa ccatgttccg cccgccgtca atgcccaggg tggagacgat
     4801 cgcggcggct catggtcgcc ggtgtccgag ggatcgtccg gagggccgcg tcctactggt
     4861 cgtcctctgt cagctgggcg gctcgggtct cgaggtagcg ccgttcgggg aggctggtgg
     4921 tgcggcgcgc ggcctgccgg tagcaggcgc gggcggcttc gtggtcccct gccatctcca
     4981 gcaggtgggc acggaccgcg tcgaggcggt gatgcccggc cagccgttcg tcggcgtcca
     5041 ggctgtcgag caggtccagg ccggcctggg ggccgtgcac catcgcgacg gcgacggcgt
     5101 ggttgagggt gaccatgggg ttgggcgcga tgcgctcgag cagctggtac agggcgagga
     5161 tctgcggcca gtcggtgtcc cgcgcccgtg ccgcctcgtc gtggaccgcg gcgatcgccg
     5221 cctgcagctg gtaggggccg agcggcgccc gcgtcagcgt gtcggtgatg agcgccacgc
     5281 cctcccggat ggcttcctgg tcccaccgcc cccggtcctg ctcagccagc gggaccaggc
     5341 tgccgtccgg ccgggtgcgt gccggacgcc gagcgtcggt gagcagcatc agcgccagca
     5401 gcccggtcac ctcgccgtca tccggcagca gtcgccggac ctcccgggca agccggatcg
     5461 cctcgccggt cagctcggcg cggtgc
//
LOCUS       DASPYY010000285         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1314730, whole genome shotgun sequence.
ACCESSION   DASPYY010000285 DASPYY010000000
VERSION     DASPYY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314730"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VEP73_04400"
     CDS             <1..950
                     /locus_tag="VEP73_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYT25710.1"
                     /translation="EPAGSPLTERLRARVRWPPDALRPQDAGDPLFPEGPAAAVELAR
                     ADPSALPARVPPGEADAFAAARSAQRHLVATSHAQGPPPRPMPPRILSVSQVLTYDRC
                     PRAFYWAVVRPLPSPPHPAARLGTVVHRLLERRARALLDLVDVGDLGGEAAAGSTPGP
                     GLVERATRNFAATRYARLPAPEAEVGVALRSGPWVVRGRIDAIFRDGSGVELVDWKTG
                     RPTPAGPDRLDQLGLYALALRELGQLPGGGCTASYCHLGGQAPEVHSRRYGPAELDGQ
                     RALLEAVLDGIEQGRYERFCGRPECEACGPLGPPSPVRR"
     gene            1096..1866
                     /locus_tag="VEP73_04405"
     CDS             1096..1866
                     /locus_tag="VEP73_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT25711.1"
                     /translation="MSPAMSSAMSTAERGERPDPGRGATRERILVLLRRHGGLSAPRL
                     AGLLDLTTVGVRRHLAVLERDGLVTASVEKPKRGRPTAIYRLTDAGHETFPRRYDEMA
                     REALTFLKGQDAAVLSRFLAWRNERLAASYADRVEGATLEERAEALAEVLSQQGYMAE
                     VETSPGGLRLCQHNCTVEHLASELPDLCASEAKLFELLLGAPVRRETTIARGAVRCVT
                     QVDVPGNARRHTAARTTPSPTLDATTAAPAREATRSQA"
     gene            1935..3314
                     /gene="sufB"
                     /locus_tag="VEP73_04410"
     CDS             1935..3314
                     /gene="sufB"
                     /locus_tag="VEP73_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYT25712.1"
                     /translation="MGAYKFGWHDPVHSVFTPKKGLSHDVVEEISAMKSEPEWMRKFR
                     HKALDAYYRKPMPQWGGDLNGIDFDNIYYFLRATEKQAESWEDLPADIRNTYDRLGIP
                     EAEKQRLVSGVAAQYESEVVYHKVREDLEAQGVLFLDTDSALREHEDLFREHFATVIP
                     AADNKFAALNSAVWSGGSFIWIPEGVRVEIPLQAYFRINSQNMGQFERTLIIAEPGSS
                     VHYVEGCTAPIYSSDSLHSAVVEIIVKPHAKVRYTTIQNWSNNVYNLVTKRAAAYEGA
                     TMEWIDGNIGSKLTMKYPAVLLLGEHAHGEILSVAFAGDGQHQDAGAKLTFAAPNTTG
                     QIISKSISRDGGRTSYRGLIKVEEGSPGVKANVRCDALLLDEESRSDTYPYTDVKEED
                     VTLGHEATVSKIGDDQLFYLRSRGLSEAEATAMIVRGFIEPISKELPMEYALELNRLV
                     ALQMEGAVG"
     gene            3317..>4239
                     /locus_tag="VEP73_04415"
     CDS             3317..>4239
                     /locus_tag="VEP73_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HYT25713.1"
                     /translation="MTETSTETLTLKGLDRAAVTAWSEALGDPEWLLARRLEALAALE
                     KLERPGGREEAWRFTDPKRAGLDRHRILRHAGRTRPAESGTGVFAGDAAHGRRRDTDL
                     EPGGLVTTIDGATVEATLSADLAAKGVILTDLATAAREHPGIVEPRLMTTAAPFDEDW
                     FLALHGTLVTAGTFLYVPRGVEVEVPIGARYRRTTAGATFGHTLVVVGEGAALTFVQQ
                     HDSPEEIDGPAFHHGVTELFVGPDASVQYLALQEWGSERVNHFGVQRAEVARQGRFRS
                     FVVTLGGGVVRISPDVRLHEGAEADLLGAVFA"
BASE COUNT          607 a         1624 c         1469 g          539 t
ORIGIN      
        1 aggagccggc cgggagcccg ctgaccgagc ggctgcgggc ccgcgtgcgg tggccgcccg
       61 acgccctgcg gccccaggac gccggcgacc cgctcttccc cgaaggaccg gcggcagcgg
      121 tcgagctcgc gcgggcggac ccgtccgcgc tgcctgcccg ggtgccgccc ggcgaggctg
      181 acgccttcgc ggccgcgcgc tccgcccagc gccacctggt ggcgacgagc catgcccagg
      241 gccccccgcc ccggccgatg cccccgagga tcctctcggt cagccaggtg ctcacctacg
      301 accgttgccc ccgcgcgttc tactgggcgg tcgtccggcc gctgccgtcg ccgccgcacc
      361 cggccgcgcg gctcggcacg gtcgtccacc ggctcctcga gcggcgcgcg cgggcgctcc
      421 tggacctggt cgacgtcggc gacctgggcg gcgaggcagc cgccgggtcg acgcccgggc
      481 ccggcctggt cgaacgggcc acccgcaact tcgcggccac ccggtacgcg cggctgcccg
      541 cccccgaggc cgaggtcggc gtcgcgctgc gctccgggcc ctgggtggtg cgcggccgca
      601 tcgacgcgat cttccgcgac ggctcgggcg tcgagctcgt cgactggaag accggccggc
      661 cgacgccggc cgggccggac cgcctcgacc agctcggcct ctacgccctc gcgctccgcg
      721 agctcggcca gctcccaggc ggcggctgca cggcctccta ctgccacctg ggcgggcagg
      781 cgcccgaggt ccactcgcgc cggtacggcc cggccgagct cgacgggcag cgcgccctgc
      841 tcgaggcggt gctggacggc atcgagcagg gccggtacga gcgcttctgc ggcaggccgg
      901 agtgcgaggc gtgcggtccc ctcggccccc cctcccccgt gcgccgctga cctcccggcc
      961 cggcgcggga ccggtcgtcg gcgggccgcc gcgcctggtt gccgtgagcc ggtggggtgc
     1021 gtagactcgc acaaggaaac acgaatgttt ccaaagttct gtccagggca acgtcccctc
     1081 cagggaggga ccgctatgtc ccccgccatg tcctccgcca tgtctacggc cgagcgcggc
     1141 gagcggccgg acccgggccg cggcgccacc cgggagcgca tcctcgtgct cctgcgccgc
     1201 cacgggggcc tctccgcacc ccggctggcc ggcctgctcg acctcaccac ggtcggcgtc
     1261 cgccgccacc tcgccgtgct ggagcgcgac gggctcgtca ccgcctcggt ggagaagccg
     1321 aagcgggggc ggccgaccgc gatctaccgg ctgaccgacg ccggccacga gacctttccc
     1381 cgccgctacg acgagatggc ccgcgaggcg ctcaccttcc tcaaggggca ggacgccgcc
     1441 gtgctcagcc gcttcctcgc ctggaggaac gagcgcctgg cggccagcta cgccgaccgc
     1501 gtcgagggcg ctacgctaga ggagcgggcc gaggccctgg ccgaggtcct cagccagcag
     1561 gggtacatgg ccgaggtcga gacgtccccg ggcggcctgc gcctctgcca gcacaactgc
     1621 acggtcgagc acctggcctc cgagctgccc gatctctgcg cgtccgaggc gaagctgttc
     1681 gagctgctgc tcggggcgcc ggtcaggcgg gagaccacca tcgcccgcgg ggccgtgcgc
     1741 tgcgtgaccc aggtggacgt gcctggcaac gcgagacgcc acaccgcagc acgaacgacg
     1801 ccgtccccca ccctggacgc gaccacggcc gcgcccgccc gcgaagcaac gaggagccaa
     1861 gcctgatggc cactgaagcc cgtgaactcc agccgtctcc ggagcggatc aaccccgtgc
     1921 cggagaacgt cgacatgggc gcctacaagt tcggctggca cgacccggtc cactcggtct
     1981 tcacgccgaa gaagggcctg tcccacgacg tcgtcgagga gatctcggcg atgaagagcg
     2041 agcccgagtg gatgcggaag ttccgccaca aggcgctcga cgcctactac cgcaagccga
     2101 tgccgcagtg gggcggggac ctgaacggca tcgacttcga caacatctac tacttcctgc
     2161 gcgcgaccga gaagcaggcg gagtcctggg aggacctgcc ggccgacatc cgcaacacct
     2221 acgaccggct gggcatcccc gaggcggaga agcagcgcct ggtctccggc gtcgccgccc
     2281 agtacgagag cgaggtggtg taccacaagg tgcgcgagga cctcgaggcc cagggtgtcc
     2341 tcttcctcga caccgacagc gcgctgcgcg agcacgagga cctcttccgc gagcacttcg
     2401 cgacggtgat ccccgccgcc gacaacaagt tcgcggcgct gaactcggcc gtgtggtcgg
     2461 gcgggtcgtt catctggatc cccgagggcg tccgcgtcga gatcccgctg caggcctact
     2521 tccgcatcaa ctcccagaac atgggccagt tcgagcggac actgatcatc gccgagcccg
     2581 gctcgagcgt gcactacgtc gagggctgca ccgcgccgat ctactcgtcg gactcgctgc
     2641 actcggccgt ggtcgagatc atcgtcaagc cccacgccaa ggtccgctac acgaccatcc
     2701 agaactggtc gaacaacgtc tacaacctgg tcaccaagcg ggccgcggcc tacgagggcg
     2761 cgaccatgga gtggatcgac ggcaacatcg ggtcgaagct cacgatgaag tacccggccg
     2821 tgctgctgct cggcgagcac gcccacggcg agatcctctc ggtcgcgttc gccggcgacg
     2881 gccagcacca ggacgccggg gccaagctca cgttcgcggc gcccaacacg accgggcaga
     2941 tcatctccaa gtcgatctcg cgcgacggcg ggcgcacctc ctaccgcggt ctcatcaagg
     3001 tcgaggaggg ctcgccgggc gtgaaggcca acgtgcgctg cgacgcgctg ctgctcgacg
     3061 aggagtcgcg ctccgacacc tacccgtaca ccgacgtgaa ggaggaggac gtcaccctcg
     3121 gccacgaggc gacggtctca aagatcggtg acgaccagct cttctacctc cggtcgcgcg
     3181 ggctctctga ggcggaggcg accgccatga tcgtccgcgg cttcatcgag ccgatctcca
     3241 aggagctccc gatggagtac gcgctcgagc tcaaccgcct cgtcgccctg cagatggaag
     3301 gggcggtcgg gtaagcgtga ccgagacgag caccgagacg ctgacgctga aaggcctcga
     3361 ccgggcggcc gtgaccgcct ggtcggaggc gctcggcgac cccgaatggc tcctggcccg
     3421 ccggctcgag gccctcgccg ccctcgagaa gctggagcgg ccgggcggcc gcgaggaggc
     3481 gtggcggttc accgacccca agcgggccgg cctcgaccgg caccgcatcc tccgccatgc
     3541 cggccggacc cggccggccg agtcgggcac gggcgtgttc gccggcgacg ccgcgcacgg
     3601 caggcggcgc gacaccgacc tcgagccggg cgggctggtg accaccatcg acggcgccac
     3661 cgtcgaggcg accctgtccg ccgacctcgc cgccaagggc gtgatcctca ccgacctcgc
     3721 gaccgcggct cgcgagcacc ccggcatcgt cgagccgcgc ctcatgacca cggcggcgcc
     3781 gttcgacgag gactggttcc tcgcgctgca cggcacgctg gtcaccgccg gcacgttcct
     3841 ctacgtcccc cgcggcgtcg aggtcgaggt gccgatcggc gcccgctacc ggcgcaccac
     3901 ggctggcgcg accttcggcc acaccctcgt cgtggtcggg gagggggctg cgctcacgtt
     3961 cgtgcagcag cacgactcgc ccgaggagat cgacgggccc gccttccacc acggcgtcac
     4021 cgagctcttc gtcgggccgg acgcgtccgt gcagtacctc gcgctccagg agtggggcag
     4081 cgagcgggtc aaccacttcg gcgtccagcg cgccgaggtc gcccgccagg ggcggttccg
     4141 ctcgttcgtg gtcaccctcg gcggcggagt ggtccgcatc tcgcccgacg tgcgcctgca
     4201 cgagggcgcg gaggccgacc tcctcggtgc cgtcttcgc
//
LOCUS       DASPYY010000286         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2157376, whole genome shotgun sequence.
ACCESSION   DASPYY010000286 DASPYY010000000
VERSION     DASPYY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157376"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="tig"
                     /locus_tag="VEP73_04420"
     CDS             complement(<1..972)
                     /gene="tig"
                     /locus_tag="VEP73_04420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYT25714.1"
                     /translation="MRSTVEPVEPTKLKLNIVVEPDELKPAIDRTARRLANQVKVPGF
                     RKGKVPRQVIEARIGRGALVAEAIEHEAVPEFYARAVEEHGVAPLSRAQVEQPSYEDG
                     GPLEFSATVEVKPTLDLPPYRGVQVERPVVEVTDEMVDARIEQLRERFAQLEVIGRPL
                     AAGDYAQIDLRASRHTTEIPALTQTDFLYEVGSGRVVPKLDEELEGTRRGDILRFTVT
                     LPEGLASEELVGQDVTFQVLVKEAKAKVLPKLDDDFAQEASEFDTLEELRAAERERLE
                     AAARQQAGSELETRVLEAYLGQVDVPLPDSLVRDELGYRANRVAQQLA"
     gene            complement(1333..1533)
                     /locus_tag="VEP73_04425"
     CDS             complement(1333..1533)
                     /locus_tag="VEP73_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25715.1"
                     /translation="MEVTAHPWPRTTVVILLALALLVVSLAGISYSSRVSTVEGPCRH
                     AYDLSQAKRHDVPYGVYRKTFC"
     gene            1880..2491
                     /locus_tag="VEP73_04430"
     CDS             1880..2491
                     /locus_tag="VEP73_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25716.1"
                     /translation="MRLVRTVAIVATVAALTVAFAVAPASAQTVPTLVAVRTGHHPGF
                     DRVVFELRGGRPSAQVRYVRQLTEDASGKPVSLAGSANLLVVFHGVNAHDDSGQPTVS
                     PRRFSPNLPNLKEVAQIGDFEAVVSYGLGLDHRVPVRVFNLSSPSRVVIDIPTGGQAQ
                     ATAGQGGTLPFTGSNDAALLVTGIAMLLAGGIALVLGRITRTP"
     gene            2710..3147
                     /locus_tag="VEP73_04435"
     CDS             2710..3147
                     /locus_tag="VEP73_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT25717.1"
                     /translation="MGLTERERAFLEQHPSAAMVTLRDDGSPHVVRVGAALVDGKVWS
                     SGKKGRVRTGHLRRDPRSTLFVFDGQWRWLALDSTVTILEGADAPEMSLRLFQAMQRR
                     LGPGPGNVIWEGTERSPEEFLRIMVEEQRLIYQFEVIRSYGLY"
     gene            complement(3188..>3700)
                     /locus_tag="VEP73_04440"
     CDS             complement(3188..>3700)
                     /locus_tag="VEP73_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HYT25718.1"
                     /translation="RLKAEAAQLNKLVLQTGPLLAQLRRADPTNPLLPTANGADRPGG
                     PVVLPASWGTGRAWAAIRYAYAQLGKPYVWGATGPAAFDCSGLTLMAWAQAGVALPHF
                     AAAQFAIGRPVTRSELQPGDLIFRNSPISHVAIYVGSGLQIAATHTGSTVKLQAAFSG
                     DIVGYTRPDG"
BASE COUNT          475 a         1321 c         1379 g          525 t
ORIGIN      
        1 cgcgagctgc tgcgcgacgc ggttggcccg gtaccccagc tcgtcgcgca ccagcgagtc
       61 gggcagcggg acgtcgacct gcccgaggta ggcctcgagc acccgcgtct cgagctcgga
      121 cccggcctgc tgccgcgcgg ccgcctcgag gcgctcccgc tcggcggcgc gcagctcctc
      181 gagggtgtcg aactcgctcg cctcctgggc gaagtcgtcg tcgagcttgg gcaggacctt
      241 ggccttggcc tccttgacga gcacctggaa ggtgacgtcc tgcccaacca gctcctcgct
      301 cgccaggccc tcgggcagcg tcaccgtgaa gcgcaggatg tcgcccctgc gggtgccctc
      361 gagctcctcg tccagcttgg ggacgacgcg gccggagccg acctcgtaga ggaagtcggt
      421 ctgggtgagc gcggggatct cggtggtgtg gcgggaggcg cgcaggtcga tctgggcgta
      481 gtccccggcg gccagcgggc ggccgatgac ctcgagctgg gcgaagcgct cacggagctg
      541 ctcgatccgg gcatccacca tctcgtcggt gacctcgacg accggccgct ccacctggac
      601 gccccggtag ggcggcaggt cgagcgtcgg cttgacctcg accgtcgcgg agaactcgag
      661 cgggccgccg tcctcgtacg acggctgctc gacctgggcg cgggagagcg gcgcgacgcc
      721 gtgctcctcg accgccctgg cgtagaactc gggcaccgcc tcgtgctcga tcgcctcggc
      781 gaccagggcg ccgcggccga tccgggcctc gatgacctgg cggggcacct tgcccttccg
      841 gaacccgggg accttgacct ggttggccag ccggcgagcg gtccggtcga tggctggctt
      901 gagctcgtcg ggctcgacca cgatgttgag cttcaacttg gtgggctcga cgggctcgac
      961 ggtactgcgc acggatgcct ccggaggacg ggtacggcgg gatcgactct ggtcaaggga
     1021 gcaggtcagg cgagcatcgg cccgggaggt gcggcggcgc cggaccgggg agagccacgc
     1081 ggggcgggcg gcgcgggcca gtatacccgc ccccccggcc ggctcggtcc ccccggcgtc
     1141 tccttcgaac ggcccgagcc cgccgcgcgg attcaggttt cccaggcgcc gatgaaaccg
     1201 gagggggggc cagtgccgaa cactgtgtgg gggggcaccc gtggggggca cagggcggcg
     1261 gctcggtggt cggaaggccg cgagccgccg ccttcaccgg ccgccggcgc cgcctcgcgg
     1321 cgccggtccc cgtcagcaga acgtcttccg gtagaccccg tagggcacgt cgtggcgttt
     1381 cgcctggctc aggtcgtagg cgtggcggca cggcccctcc accgtgctga cccgggaact
     1441 gtacgagatc ccggcgaggg agacgaccag cagcgcgagc gcaagcagga tcaccacggt
     1501 cgtccgcggc cacgggtgcg ccgtcacctc caccggcgcc acgtaccccg gctcgcccca
     1561 gcggggcggg cgccggctgg gccagctggt ggtgctccac ctccacgtca ccggcgggag
     1621 cgtcctgtgg ggtcgggatg ccacgctcgc ggcctcctcc gtcgcgccgg ccgcctgccc
     1681 gacggccggt cgcactggtt gcattcccgc cggaccgggc gcatacacgc cggggcgacg
     1741 gcggccggga ccggggtccg gcggcccgct gtgatcaatt cgaggcgaga cggcatccgc
     1801 acggaggccc atccgtggtc gtggtgtgac cgggccggcg catcgtccaa gcgactgctg
     1861 ccaaggaagg gtggtcgcca tgcggctcgt ccggaccgtc gccatcgtcg ctaccgtcgc
     1921 cgctctcacc gtcgccttcg ccgtcgcgcc ggccagcgcc cagaccgtgc cgaccctcgt
     1981 cgcggtgcgc accggccacc accccggctt cgaccgggtc gtcttcgagc tccgcggggg
     2041 ccggcccagc gcgcaggtcc gctacgtccg ccagctcacc gaggatgcct ccggcaagcc
     2101 ggtgtcgctg gccggcagcg ccaacctgct ggtcgtcttc cacggggtca acgcccacga
     2161 cgacagcggc cagccgacgg tgtcgccgcg ccgcttctcc ccgaacctgc ccaacctgaa
     2221 ggaggtcgcc cagatcggcg acttcgaggc cgtggtcagc tacgggctcg gcctcgacca
     2281 ccgggtcccg gtcagggtgt tcaacttgtc gtccccgtcc cgggtcgtga tcgacatccc
     2341 gaccggcggc caggcccagg ccacggccgg ccagggcggc acgctgccgt tcaccggctc
     2401 caacgacgcc gccctgctcg tcaccggcat cgccatgctg ctggccggcg ggatcgcact
     2461 ggtcctcggc cgcatcaccc gcacgccctg acggctccat ggtccttggc cccggcggcc
     2521 cgccggggcc aaggacgcgt atcccgtgcc cttcggcggc gagcctcgac cagacctggt
     2581 gcggcgtcac cggtcaagcg gcaggttgcg caggctgacg gccttgccgc agcgatgcgg
     2641 tccggtgccg cgggcgtacg ctgccggtca cctcaccagg gcacgtccac tcggaggaag
     2701 gggaggcgca tgggtcttac ggagcgggag cgggccttct tggagcagca ccccagcgcg
     2761 gcgatggtca cgctcaggga cgacgggagt ccccatgtgg tccgggtcgg tgcggcactg
     2821 gtcgacggca aggtctggag cagcgggaag aagggccggg tccgcacggg gcatctgcgg
     2881 cgggaccccc gctccaccct gttcgtgttc gacgggcagt ggcgatggct cgcgctggac
     2941 tccaccgtga ccatcctcga aggtgctgac gccccggaaa tgagcctgcg cctgttccag
     3001 gcgatgcagc ggcggctcgg ccccggcccc gggaacgtca tctgggaggg gacggagcgc
     3061 agccccgagg agttcctgcg gatcatggtg gaggaacagc gactgatcta ccagttcgag
     3121 gtcatccgtt cctacgggct gtactgattc ccccgggggc ctgaccctgg ggaggtggtc
     3181 ggtggcgtca gccgtcgggg cgggtgtagc cgacgatatc gccgctgaag gcggcctgta
     3241 gcttgaccgt gctgccggtg tgcgtagctg cgatctgcag tccgctgccc acgtagatcg
     3301 ccacgtggga gatggggctg ttgcggaaga tgaggtcgcc gggctgcagc tcgctgcggg
     3361 tgacgggacg gccgatggcg aactgggcgg ccgcgaagtg gggcagggcg acgccggcct
     3421 gcgcccaggc catcagcgtc agcccggagc agtcgaacgc cgcgggaccg gtcgcgcccc
     3481 acacgtaggg cttgccgagc tgggcgtagg cgtagcggat ggccgcccac gcccggcccg
     3541 tgccccagct cgccggcagc acgaccgggc cgccaggccg gtcggcaccg ttggcggtag
     3601 gcagcagcgg gttggtcggg tcggctcgcc ggagctgggc gagcagcggc ccggtctgca
     3661 ggacgagctt gttgagctgc gctgcctccg ccttgaggcg
//
LOCUS       DASPYY010000287         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_752796, whole genome shotgun sequence.
ACCESSION   DASPYY010000287 DASPYY010000000
VERSION     DASPYY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752796"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VEP73_04445"
     CDS             complement(<1..358)
                     /locus_tag="VEP73_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25719.1"
                     /translation="MRRKVSLLVGVSLMALLVAAPAASARSNAAGSAKAGGLFGSRAQ
                     MVRYVRTHGYIPIGGPAAYARYSAAAAQRAAALHPGTYRGPVGTAPVASPSWQGVDEN
                     DLAPPDPTGAIGTKSYV"
     gene            complement(493..1494)
                     /locus_tag="VEP73_04450"
     CDS             complement(493..1494)
                     /locus_tag="VEP73_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYT25720.1"
                     /translation="MILAFVEHDRGTVEEGSFEALTLGRRLAERLSVPLHALVVGDGA
                     RPLAARAAAFGATATHVAVHARLDGYAPEAWAACLADRIGSLGPLAVLAAATDRGNEI
                     LAHAAARSGLPMAANCIRVEPGEPGDPADPSTPWTLTRLQWGGSLLEEARLSGGVKLF
                     TVAPHVVAAEPVASPAGHQVLPFEPVLDERDLRARVTATVEAPAGKISLPAARVVVGG
                     GRGVGSAAGFAALEELADLLGGAVGCSRVVTSLAWRPHADQVGQTGTRIAPDLYIACG
                     ISGAIQHMVGCKGAKHLLAINTDPDAPIMAKAEYAVIGDVQEVLAALNAALHATARG"
BASE COUNT          205 a          630 c          602 g          206 t
ORIGIN      
        1 ggacgtagct cttggtcccg atggcacccg tcgggtccgg cggtgccagg tcgttctcgt
       61 ccacgccctg ccagctgggg ctcgccacgg gcgcggtccc gaccggcccg cgatacgtgc
      121 ccggatggag cgcggcggcc ctctgcgcgg cggcggcgct gtagcgcgcg tacgcggccg
      181 ggccgccgat cgggatgtag ccgtgcgtgc gtacgtaccg gaccatctgt gcgcgcgagc
      241 cgaacaagcc gcccgctttc gcggagcccg cggcgttcga cctggccgaa gccgcgggcg
      301 cggcgaccag gagtgccatg agcgagaccc ccaccagcaa cgagaccttc cgtctcacgg
      361 cccctccttc cgggactgat cccttaccgg ctcttccgcc ggccgcccca cacggccggt
      421 cgccgggcgg agcgagggcc ccaacgtacc ccggcgacat gaaccggctg tgagagcgcg
      481 gtgagaggtg tcttacccgc gtgccgtcgc atgcagggcc gcgttgaggg cggccaggac
      541 ctcctggacg tcgccgatca ccgcgtactc cgccttcgcc atgatcggcg cgtcggggtc
      601 ggtgttgatg gcgaggaggt gtttcgcgcc cttgcacccc accatgtgct ggatggcgcc
      661 gctgatcccg caggcgatgt agaggtcggg cgcgatccgc gtacccgtct ggccgacctg
      721 gtcggcgtgg gggcgccacg ccaggctcgt gacgacccgc gagcagccca ccgccccgcc
      781 caggagatcg gccagctctt ccagcgcggc gaacccggcc gcgctcccga cgccccggcc
      841 gccgcccacg accacgcggg cggccggcag ggagatcttg ccggccgggg cttcgaccgt
      901 ggcggtgacc cgcgcacgca ggtcccgctc gtcgaggacc ggctcgaacg gcagcacctg
      961 gtgccccgcg ggcgaggcga ccggttcggc ggcgaccacg tgcggggcca ccgtgaacag
     1021 cttgaccccg cccgacaggc gggcttcctc cagcaagctc ccgccccact ggaggcgggt
     1081 gagcgtccac ggggtgctcg gatcggccgg gtctcccggt tcaccgggct cgacgcggat
     1141 gcagttcgcc gccatcggca ggcccgaccg ggcggcggcg tgggcgagga tctcgttccc
     1201 ccggtcggtg gcggcggcca ggacggcgag cgggcccagg gacccgatcc gatcggcgag
     1261 gcacgcggcc catgcctcgg gcgcgtaccc gtcgaggcgc gcgtgcaccg cgacgtgcgt
     1321 ggccgtggcc ccgaacgcgg ccgcccgcgc ggccagcggc cgggccccgt ctcccaccac
     1381 gagggcgtgg agcggaacgc tcagcctctc ggccagccgc cggccgagcg tgagcgcctc
     1441 gaacgatccc tcctccaccg tcccgcggtc gtgttcgacg aacgccagga tcacggtggc
     1501 agccccagcg accgcaggac ctcgacgatc cgagggaccg cctccggccc ccggccgagg
     1561 atctccaccg agctgtccgg ctccttcggc agcttcaggc ggatcgtctg caggccgccg
     1621 ggcgcggcgg gcggcgagga ccg
//
LOCUS       DASPYY010000288         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2299416, whole genome shotgun sequence.
ACCESSION   DASPYY010000288 DASPYY010000000
VERSION     DASPYY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299416"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VEP73_04455"
     CDS             complement(<1..180)
                     /locus_tag="VEP73_04455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935811.1"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYT25721.1"
                     /translation="MRVPSQNLTGDSPAQLRARAAAKINLALQVGAVREDGYHEVVTV
                     LQAVGLWDELEVHLVP"
     gene            complement(217..1131)
                     /gene="rsmA"
                     /locus_tag="VEP73_04460"
     CDS             complement(217..1131)
                     /gene="rsmA"
                     /locus_tag="VEP73_04460"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYT25722.1"
                     /translation="MPPESPGPVPAAGDTPAGPRLLTPPLVRGLLTRHGLRPTKSLGQ
                     HYLADPNTARKVARLAGVHPGETVLEVGPGLGSLTLALREAGARVVAVEVDPRLVPAL
                     AEVLAGDPGVTVAVADAMRADLGALAPGAGRLVANLPYNIAAPLVLRALVAYPHLADM
                     TVMVQREVGERLAAAPGAAAYGGPSAKVAALAEARVLAPVSRRVFVPEPHVDSVLVGV
                     HRRPHPALSGLAWAAFARVVDAAFSQRRKTLRNSLRTLGLDPGAVEALGARAGVDLAL
                     RAERLGVEEFAALAGALGEPGAGPPGRA"
     gene            complement(1138..>1722)
                     /locus_tag="VEP73_04465"
     CDS             complement(1138..>1722)
                     /locus_tag="VEP73_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYT25723.1"
                     /translation="NEAEQLTPEAVAGLRELLARPEVVGVGETGLDYYRDRCPRDAQR
                     AAFATHVRLARELDRVLVVHCRDAFEDALAVLDGEGAPERVVFHCFTGDERIAGRVVE
                     AGYLVSFSGTVTFRNAPGQRAACAVVPLDRMVLETDSPFLSPHPYRGQPNRPWRVAVT
                     AETVAAVHRTTVDEVARATTATAVRAFRLPAPVG"
BASE COUNT          202 a          629 c          707 g          184 t
ORIGIN      
        1 ggggacgagg tggacctcga gctcgtccca gagcccgacg gcctgcagga ccgtgacgac
       61 ctcgtggtag ccgtcctccc ggaccgcccc gacctggagg gccaggttga tctttgccgc
      121 cgcccgtgcg cggagctggg cgggcgagtc ccccgtcagg ttctggctgg gcacgcgcat
      181 tcctcctcgg ggcggcgggg ggtcgggggt ttcaggctac gcccgtccgg gcggtccggc
      241 gcccggctcg ccgagggcgc cggcgagggc ggcgaactcc tcgacgccga gccgctcggc
      301 cctgagggcg aggtcgaccc ctgcgcgggc accgagcgcc tcgaccgctc ccgggtcgag
      361 gccgagggtg cgcagcgagt tgcgcagcgt cttgcgccgc tgggagaacg cggcgtcgac
      421 gacccgggcg aaggccgccc aggccaggcc ggagagggcc gggtggggac ggcggtgcac
      481 gccgacgagc accgagtcga cgtgcggctc ggggacgaac acgcgccggc tcaccggggc
      541 gagcacccgc gcctcggcga gcgcggccac cttcgcgctc ggccccccgt acgcggccgc
      601 gccaggcgcg gcggccagcc gctcccccac ctcccgctgc accatgacgg tcatgtcggc
      661 gaggtgcggg taggccacga gcgccctgag caccaggggc gcggcgatgt tgtagggcag
      721 gttggcgacc agccgccccg ctcccggcgc gagcgcgccg aggtcggcgc gcatggcgtc
      781 cgccaccgcg acggtgacgc cgggatcgcc ggccaggacc tcggccaggg cggggaccag
      841 gcgcgggtcg acctcgacgg cgacgacgcg ggcgcccgcc tcgcggagcg cgagcgtgag
      901 gctgccgaga ccgggcccga cctcgagcac ggtctcgccc gggtggacgc cggcgagccg
      961 ggccaccttg cgggcggtgt tcgggtcggc caggtagtgc tgcccgagcg acttcgtcgg
     1021 ccgcagccca tggcgggtga gcagcccgcg caccaggggc ggggtcagca gccggggccc
     1081 ggccggggtg tcgccggcgg cggggacggg tccggggctc tcgggcggca cgtcgcctca
     1141 gccgaccggc gcggggagcc ggaacgcccg cacggcggtc gcggtggtcg cgcgggcgac
     1201 ctcgtccacc gtcgtccggt gcacggcggc cacggtctcg gcggtcaccg cgacgcgcca
     1261 gggccggttg ggctggcccc ggtacgggtg cgggctgagg aaggggctgt cggtctcgag
     1321 caccatgcgg tcgagcggca cgacggcgca cgcggcccgc tgcccggggg cgttgcggaa
     1381 cgtgaccgtg cccgagaagg agacgaggta gcccgcctcg accacccggc cggcgatccg
     1441 ctcgtcaccg gtgaagcagt ggaacacgac ccgctccggg gcgccctcgc cgtccaggac
     1501 ggcgagggcg tcctcgaagg cgtcccggca gtggacgacg agcacccggt ccagctcccg
     1561 cgcgaggcgg acgtgggtgg cgaaggcggc gcgctgcgcg tcgcgggggc agcggtcgcg
     1621 gtagtagtca aggccggtct ctccgacgcc gacgacctcc gggcgggcga gcagctcgcg
     1681 cagcccggcc acggcctccg gggtgagctg ctccgcctcg tt
//
LOCUS       DASPYY010000289         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1878807, whole genome shotgun sequence.
ACCESSION   DASPYY010000289 DASPYY010000000
VERSION     DASPYY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878807"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEP73_04470"
     CDS             complement(<1..200)
                     /locus_tag="VEP73_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HYT25724.1"
                     /translation="MSRAAAVANGPPPGSNSPSRSRTRWATEVPGPAPEDYGVSLAWV
                     RELAEYWKDGFDWRAYEARLNS"
     gene            131..>1780
                     /locus_tag="VEP73_04475"
     CDS             131..>1780
                     /locus_tag="VEP73_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HYT25725.1"
                     /translation="MFGSGSASSTPAAARSPPPPPWTRPRTAIWPGPRRGARRSCSPT
                     TAFSPWPDPSARSLRPSPSSAPSPTPSTRTGTARRCPTGSRSPTASPRPARPSPCTRA
                     PTGVRTDLGEFDVFDWGDDVITLRGAQSGKYLTVTGDGTLAVTAGKPDGWTVRETFRR
                     TLAPGGAALLESTATGQAFRLRWDPLSDGVAGAAAAARDAEVAVVVVGNNPMIGGREA
                     RDRATLALPPSADRLVREVTAASPRTVLVIMSSYPYVTPDARAIIWTCHAGQETGHAL
                     ADLITGRHAPEGRLPQTWYAADADLPDPLDYDIIKAGWTYQYTTARPRFPFGHGLTYT
                     TFGYGPLRVEAPLEESTVPAEGSAPEQVTASLDVTNAGDRDGTEVVQLYASYAGPDQP
                     RRRLCGFTRVTLEPGETATARISVPLERLRLWDVAAGRMTVPPGPVEIGAGASSADIR
                     QTATLPLPAASPVRRPARVAAADFDDYANVTLTDTTREAGTAVTPADPALPAWLKFTS
                     IHSERSDVAAFRTACSGLSGGRIEVWLAPPGDGKSPFASVSV"
BASE COUNT          232 a          716 c          607 g          225 t
ORIGIN      
        1 taggagttga gccgcgcctc gtaagcgcgc cagtcgaagc cgtccttcca gtattcggcc
       61 agctcccgca cccaggccag gctcactccg tagtcttccg gcgccgggcc ggggacttcg
      121 gtcgcccacc gtgttcggct ccggctcggc gagttcgacc ccggcggcgg cccgttcgcc
      181 accgccgccg ccctggacac gcccgcgcac cgcgatctgg ccaggaccgc ggcgcggcgc
      241 gcggcggtcc tgctctccaa cgacggcctt ctccccctgg ccggaccctt cggcccgcag
      301 cctgcgacca tcgccgtcgt cggccccctc gccgacaccc tctacgagga ctggtacagc
      361 ccggcgctgc cctaccgggt cacgatcgcc gacggcctcg ccgcggccgg cgcgaccgtc
      421 accgtgcacg agggcgccga ccggggtccg caccgacctc ggcgagttcg acgtcttcga
      481 ctggggcgac gacgtcatca cgctgcgcgg cgcgcaaagc ggcaagtacc tcacggtcac
      541 cggtgacggc acgctcgccg tcaccgccgg gaagccggac gggtggacgg tccgcgagac
      601 gttccgccgc acgctcgcgc ccggcggggc cgcgctgctt gagtcgaccg cgaccggcca
      661 ggcgttccgg ctgcgctggg acccgctgtc cgacggggtc gccggagccg ccgccgccgc
      721 cagggacgcg gaggtcgcgg tggtggtcgt cggcaacaac ccgatgatcg gcggccggga
      781 ggcccgtgac cgggccacgc tcgccctgcc cccgtcggcg gaccgcctcg tccgcgaggt
      841 caccgccgcc agcccgcgca cggttctcgt gatcatgtcc agctacccgt acgtcacgcc
      901 cgatgcccgc gcgatcatct ggacctgcca cgccggccag gagaccggcc atgccctcgc
      961 cgacctcatc accggccggc acgccccgga gggccggctc ccgcagacgt ggtacgcggc
     1021 cgacgcggat cttcctgacc cgctcgatta cgacatcatc aaggccggct ggacctacca
     1081 gtacacgacc gcgcggccgc gattcccgtt cggccacggc ctgacctaca cgacgttcgg
     1141 gtacggcccg ctgcgcgtcg aggcccccct ggaggagagc acagtgcctg cggagggcag
     1201 cgctcctgag caggtcaccg cgagcctgga cgtcacgaac gccggcgacc gcgacggcac
     1261 cgaggtcgtc cagctatacg cgagctacgc cgggcctgac cagccgcggc gccggctgtg
     1321 cggcttcacc cgggtaacgc tcgagcctgg cgagacggcg acggcacgga tcagcgtgcc
     1381 gctggaacgc ctgcggctgt gggacgtcgc ggcgggccgg atgaccgtgc cgccgggccc
     1441 ggtcgagatc ggcgccgggg cgtccagcgc cgacatccgc cagaccgcga cgctccccct
     1501 ccccgccgcg agccccgtgc ggcggcccgc gcgggtcgcg gccgccgact tcgacgacta
     1561 cgcgaacgtc accctcaccg acaccacccg cgaggccggt accgcggtca cccccgccga
     1621 cccggcgctg cccgcgtggc tgaagttcac gagcattcat tccgaacgga gcgacgtggc
     1681 tgcgttccgt accgcctgct ccggcctctc ggggggacgg atcgaggtct ggctcgcgcc
     1741 tcccggcgac gggaagagcc cgttcgccag cgtctccgtg
//
LOCUS       DASPYY010000290         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1879310, whole genome shotgun sequence.
ACCESSION   DASPYY010000290 DASPYY010000000
VERSION     DASPYY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879310"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1741
                     /gene="carB"
                     /locus_tag="VEP73_04480"
     CDS             <1..>1741
                     /gene="carB"
                     /locus_tag="VEP73_04480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYT25726.1"
                     /translation="TGSRERDVRAARKHWGVLPVFKTVDTCGGEFEARTPYYYSTYED
                     ATEVRPAERPRVVILGAGPNRIGQGIEFDYACVHAAFALREAGFETVMVNSNPETVST
                     DYDTSDRLYFEALTVEDVLAVCEAERPVGVIVQLGGQTPLAMAAELERAGVTVLGTAT
                     ESIDLAEDREKFGALLRRLGLPAPPHGTASSAEEAARVAERIGYPVLVRPSYVLGGRA
                     MEIVYSADDLSTFVESAAAASPDHPVYLDRFLEGAIEVDVDAVADGREVLVGAVMEHI
                     EEAGVHSGDSSCVIPPATLADDELDRIEETVRIIARALDVRGLLNVQLAVRGETTFVL
                     EANPRASRTVPFVSKATGMPLAKVAARVMLGATLAGLREEGLLPEHRGYRALGHVAVK
                     AAVLPFGRFPGVDTVLGPEMKSTGEVMGIAGHLGLALAKAQDATGAALPSGGSVFVSV
                     ANRDKRAIGPPARRLADLGFRILATRGTAGVLARAGVPVTPVLKRSEGSPNAVDLIAS
                     GQVGLVFNTPFGRGPRTDGYSIRTAAARAGVPCITTVPGILAAVQGIEALRRRRPEPR
                     PLQEYHAEERLVVL"
BASE COUNT          221 a          619 c          666 g          235 t
ORIGIN      
        1 accggctcgc gggagcggga cgttcgcgcc gcccggaagc actggggcgt gctgccggtg
       61 ttcaagacgg tggacacgtg cggcggcgag ttcgaggccc gcacgcccta ctactactcg
      121 acgtacgagg acgccacgga ggtccgcccc gccgagcgcc cgcgggtggt catcctgggg
      181 gcgggcccga accggatcgg gcagggcatc gagttcgact acgcgtgcgt tcacgcggcg
      241 ttcgccctgc gggaggcggg cttcgagacc gtgatggtga actcgaaccc cgagacggtc
      301 tccaccgact acgacaccag cgaccggctc tacttcgagg cgctgaccgt ggaggacgtc
      361 ctggccgtgt gcgaggccga gcggcccgtc ggggtcatcg tgcagctggg cggccagacg
      421 ccgctggcga tggccgcgga gctcgaacgg gccggcgtga cggtcctggg gaccgccacg
      481 gagagcatcg acctggccga ggaccgggag aagttcggcg ccctgctccg gcggcttggt
      541 ctcccggcgc ccccgcacgg cacggcatcg tccgcggagg aagccgcgcg ggtggccgag
      601 cgcatcggct atccggtcct ggtccggccc tcgtacgtgc tgggtggccg ggccatggag
      661 atcgtgtact cggccgacga cctgtccacg ttcgtcgagt cggccgccgc ggcgagcccc
      721 gaccacccgg tgtacctgga ccggttcctg gagggcgcga tcgaggtcga cgtcgacgcg
      781 gtcgcggacg gtcgggaggt gctggtgggg gccgtgatgg agcacatcga ggaagcgggg
      841 gtccactcgg gcgactcatc gtgcgtcatc cccccggcca cgctcgccga cgacgagctc
      901 gaccggatcg aggagacggt gaggatcatc gcccgggccc tggacgtccg cggcctcttg
      961 aacgtgcagc tcgccgtcag gggcgagacg accttcgtgc tggaggcgaa cccgcgcgcc
     1021 tcgcggacgg tcccgttcgt gtcgaaggcc accggcatgc ccctggcgaa ggtggccgcc
     1081 cgcgtgatgc tcggcgcgac gctggccggg ctgcgcgagg agggcctcct ccccgagcac
     1141 cgcggctacc gcgcgctcgg gcacgtcgcc gtgaaggcgg ccgtgctgcc cttcggccgg
     1201 ttccccggcg tcgacaccgt gctgggtccc gagatgaagt ccacgggcga ggtcatgggc
     1261 atcgccgggc acctgggcct ggcgctggcg aaggcccagg acgccaccgg cgcggcgctg
     1321 ccgtcgggcg ggtcggtgtt cgtctccgtg gcgaaccgcg acaaacgggc catcggtccg
     1381 ccggcccgcc ggctggcgga cctggggttc cgcatcctcg ccacgcgcgg cacggccggc
     1441 gtgctcgccc gggccggcgt tccggtcaca ccggtcctca agcgctccga gggcagcccg
     1501 aacgccgtcg acctgatcgc gtcgggccag gtcggcctcg tgttcaacac gccgttcggg
     1561 cgcggccccc ggaccgacgg gtattccatc cggacggccg ccgcgcgggc gggcgtgccc
     1621 tgcatcacca cggtgccggg catcctcgcc gcggtgcagg gcatcgaagc gctccgccgc
     1681 cggcggcccg agccgcggcc gctgcaggag taccatgccg aagagcgcct ggtggtgctg
     1741 g
//
LOCUS       DASPYY010000291         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_332646, whole genome shotgun sequence.
ACCESSION   DASPYY010000291 DASPYY010000000
VERSION     DASPYY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332646"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1124)
                     /locus_tag="VEP73_04485"
     CDS             complement(57..1124)
                     /locus_tag="VEP73_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT25727.1"
                     /translation="MPRNDSFPSDAPLRIAMIAPPWYPVPPDGYGGIEAMVYSLSEEL
                     TARGHEVTVIGAGKQGTSGDFLQTYVEPPSEKIGQSLPEVTHALMAAAHLEALDLDVV
                     HDHSLAGPISARERRGPTVVTAHGPVEGEMADYYSSLGPDVHLVAISEFQRERAPDLP
                     WVGVVHNAVAVDEYPFRADKEDFCLFLGRMNGEKAPDLAIEAAKAAGRRIVVAAKCSE
                     PEERRYFEKRVRPLLGPDAEWFGEADNEQKKDLLARARCLVFPIQWDEPFGLVMVEAM
                     ACGTPVVALRAGSVPEVVEDGVTGFVRDDLAELPDAIERADALDPGACRQRVVERFDV
                     PVMTDGYERVYRRLAGGPAGR"
     gene            1238..2698
                     /gene="gltX"
                     /locus_tag="VEP73_04490"
     CDS             1238..2698
                     /gene="gltX"
                     /locus_tag="VEP73_04490"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYT25728.1"
                     /translation="MSDDAGGSVRTRFAPSPTGYLHVGTARTALFSYLYARRHGGAFV
                     LRIEDTDVGRNVEGGADSIAEALHWLGLSWDEGYGVGGPHGPYVQTQRLDRYHAAVNA
                     MIAQGDAYLCYCTAAELEERRRAAEAQGRPPGYDGRCRHLTTQQVRAFEAEGRKPAVR
                     FRLPDEGETVVGDLVRGTVRFDNATLTDFVILRPNGIPTYMLSAVFDDVDMGITHVIR
                     GEDLFPSTPRQVHVFRALGRPEPPRFAHLPLLVAPDRKKLSKRRDQVSLQAFRAQGYL
                     PEAMVNYLALLGWSLDDHTEHFSLADLERSFAIERVSHNPAAFDERKLDAVNGWWIRQ
                     LDPSDLAGRALEFVRAAGYPDADPELLRQAAPLVAERVTRLDEVPGMVGFLFVEDVAL
                     DPAAAAKVLDDQGRAFLDDAAKRLESLGSWTAAAIEEVLRGLQRERGLKPKQAFQPVR
                     LAVTGTLVSPPLFESMELLGRARSLARLDRARTLVG"
     gene            2762..2833
                     /locus_tag="VEP73_04495"
     tRNA            2762..2833
                     /locus_tag="VEP73_04495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2795..2797,aa:Gln,seq:ctg)
     gene            2960..3032
                     /locus_tag="VEP73_04500"
     tRNA            2960..3032
                     /locus_tag="VEP73_04500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2994..2996,aa:Glu,seq:ctc)
     gene            complement(3386..>3510)
                     /locus_tag="VEP73_04505"
     CDS             complement(3386..>3510)
                     /locus_tag="VEP73_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25729.1"
                     /translation="YPTGVKVTDAEPAAVPLTRHDWHGEWNYTITGAPTRTTAA"
BASE COUNT          466 a         1273 c         1249 g          522 t
ORIGIN      
        1 tcgcaccgtc ggttaccgac tggtcggcat gcgcgggcgc gcgctttatc gactcctcag
       61 cgaccggccg ggccgccggc gagcctgcgg tagacccgct cgtagccgtc ggtcatgacc
      121 gggacgtcga agcgctcgac cacccgctgg cggcacgccc cagggtcgag cgcgtcggca
      181 cgctcgatcg cgtcggggag ctcggccagg tcgtcgcgga cgaagccggt gacgccgtcc
      241 tccaccactt cgggcaccga gccggcgcgc agggccacca ccggggtgcc gcaggccatc
      301 gcctcgacca tcacgaggcc gaacggctcg tcccactgga tcgggaacac caggcagcgc
      361 gcccgggcga gcaggtcctt cttctgctcg ttgtcggcct cgccgaacca ttcggcgtcc
      421 gggccgagca ggggccggac ccgcttctcg aagtagcgcc gctcctcggg ctcggagcac
      481 ttggccgcga ccacgatccg gcggccagcc gccttggccg cctcgatggc caggtcgggc
      541 gccttctcgc cgttcatgcg gccgaggaac aggcagaagt cctccttgtc ggcccggaac
      601 gggtactcgt cgaccgcgac cgcgttgtgg acgacgccca cccagggcag gtcgggcgct
      661 cgctcgcgct ggaactcgga gatggcgacg aggtggacgt ccggcccgag gctcgagtag
      721 tagtcggcca tctcgccctc gaccggtccg tgggcggtga cgacggtcgg cccccggcgt
      781 tccctggcgc tgatcgggcc ggccagggag tggtcgtgga cgacgtcgag gtcgagcgct
      841 tccaggtgcg cggcggccat cagcgcgtgg gtcacctcgg gcagcgactg cccgatcttc
      901 tcgctgggcg gctcgacgta ggtctgcagg aagtcgcccg aggtgccctg cttgcctgcg
      961 ccgatgacgg tgacctcgtg gccgcgggcg gtgagctcct cgctgagcga gtagaccatc
     1021 gcctcgatgc cgccgtagcc gtccgggggg accgggtacc aggggggcgc gatcatcgcg
     1081 atccggaggg gggcgtcgga ggggaaggag tcgttgcgcg gcacggcgtc ctcctgaccg
     1141 ctcgcggcca cccagcctgc ggtggcgaca atgctaggct tgccagcctg ggcgacggtc
     1201 aaacggcgag ttccgcgctc ctgctacgat cggcggtatg agcgacgacg ccggcggctc
     1261 ggtccgcacc cggttcgcgc cctcgccgac cggctacctc cacgtcggca cggcgcgcac
     1321 cgccctgttc agctatctgt acgcccgtag gcacggcggg gcgttcgtcc tccgtatcga
     1381 ggacaccgac gtcgggcgca acgtcgaggg cggcgccgac tcgatcgccg aggccctgca
     1441 ctggctcggc ctctcctggg acgagggcta cggcgtgggc gggccccacg ggccgtacgt
     1501 gcagacccag cggctcgacc gctaccacgc cgccgtcaac gcgatgatcg cccagggcga
     1561 cgcctacctg tgctactgca ccgccgccga gctcgaggag cggcgcaggg ccgccgaggc
     1621 gcagggccgc ccgcccgggt acgacggccg ctgccgccac ctcaccaccc agcaggtgcg
     1681 cgccttcgag gcggagggcc ggaagccggc ggtccggttc cggctccccg acgagggcga
     1741 gacggtggtc ggtgacctcg tccggggaac ggtgcggttc gacaacgcga ccctcaccga
     1801 cttcgtgatc ctgcggccga acggcatccc gacctacatg ctgtcggccg tgttcgacga
     1861 cgtcgacatg ggcatcaccc acgtgatccg gggcgaggac ctgttcccgt ccaccccgag
     1921 gcaggtgcac gtgttccgcg ccctcggccg tcccgagccg ccccggttcg cccacctgcc
     1981 cctgctcgtc gcgcccgacc ggaagaagct ctccaagcgt cgggaccagg tctcgctgca
     2041 ggcgttccgc gcccagggct acctccccga ggcgatggtc aactacctcg cgctgctcgg
     2101 ctggtccctc gacgaccaca ccgagcactt cagcctcgcg gacctcgagc ggtcgttcgc
     2161 catcgagcgg gtgtcccaca acccggccgc cttcgacgag cggaagctcg acgccgtgaa
     2221 cggctggtgg atccgccagc tcgacccgtc ggacctcgcc ggccgcgcgc tcgagttcgt
     2281 ccgcgccgcc gggtatcccg acgccgatcc cgagctgctg cgccaggccg caccgctcgt
     2341 ggccgagcgc gtcacccgtc tcgacgaggt cccgggcatg gtggggttcc tgttcgtcga
     2401 ggacgtcgcg ctcgaccccg ctgcggccgc caaggtcctc gacgaccagg ggcgcgcctt
     2461 cctcgacgac gcggccaagc ggctcgagtc gctggggtcg tggaccgcgg cggccatcga
     2521 agaggtcctg cgcgggctgc agcgggagcg cggcctgaag ccgaagcagg ccttccagcc
     2581 cgtccgcctg gccgtcacgg gcacgctggt gagcccgccg ctgttcgagt ccatggagct
     2641 gctcggccgg gcccgctccc tcgcccgcct cgaccgcgcg aggaccttgg tcggttgagc
     2701 gaaccgggcc ggagccggta cgatgcgcga gcccgtgggc cccaaggccc acgctcaccg
     2761 atggggtgtg gggtaattgg cagcccgacg ggttctggcc ccgttagtct aggttcgagt
     2821 cctggcaccc cagcagcttc cccgcggggc tgccgggctc ccgccgagct tgctggcctc
     2881 tgggatgggg cggtaccctc tgggcctgcc gcgtgaccgc agtggagccc ggcggacatg
     2941 caggtcggca cgcaggcacg gccccgtcgt ctagtggcct aggacgccgc cctctcaagg
     3001 cggtaacggc ggttcgaatc cgctcggggc tactcgaaca ccagtacgag acggcctctg
     3061 acctggacga atgatggtca ggggccgtgt cgtgtgtccg gcggcgtccg gtcaggtccg
     3121 gctgtgggcg agcatgtgtg cccgttacgt gcccgcgtcc gtgtcggcgt gcccgagagc
     3181 cgcctgccgt tgcgctcgcc gacgaacagc ccgcgaaacg cctacggctc cgcggcgccc
     3241 acgcatccgg gtccatgctg gcgcccgcca ggagcaggca cggcacagcg gtgcctgcta
     3301 cctcacaagt gcagctgggt actacgacct gttcgacggg cgtgcgatca gtaccgttaa
     3361 cggccggtta gggcttgagc cgaaattatg cggccgttgt gcgggtgggg gcacccgtga
     3421 tggtgtagtt ccactcaccg tgccagtcat gcctggtaag cggaaccgcg gcaggctcgg
     3481 cgtcggtgac cttgaccccg gtcgggtagg
//
LOCUS       DASPYY010000292         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_474976, whole genome shotgun sequence.
ACCESSION   DASPYY010000292 DASPYY010000000
VERSION     DASPYY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474976"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VEP73_04510"
     CDS             complement(<1..1031)
                     /locus_tag="VEP73_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYT25730.1"
                     /translation="MRARLGINTCFAVKRWPRPEDWARIVRDELGLEVVELSLDLIED
                     FGGGAVNQVRSALDEYGLRAETVFTGLAAYSLNLLMHPGEERRRAAEEWYLGVVDLAA
                     RLGASGAGGHVGTMSVPDWADEARRAERWAGLQHSLAAIAAAARSAGLDYLLVENLAC
                     HREPSTMAGLETLLTDGDAAHASIRLCLDVGHQCVPGTTGPDRDPYAWLTHFGGRLAE
                     VQLQQSDGLADHHWPFTAERNQAGRIDPGRVLDTLAEAGAGRSPGAGGDPGVRAGRRR
                     GAGGPAGLGRAMAGRTDRARGAMTSGFVDSNGIGIHYRIAGEAAAPTLVLINGVGDDL
                     DGWASQVDD"
     gene            complement(1028..1486)
                     /locus_tag="VEP73_04515"
     CDS             complement(1028..1486)
                     /locus_tag="VEP73_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HYT25731.1"
                     /translation="MFAPVLGDGERDDPALRGAVTGLSLRHDRGAVARAALEGIACGV
                     RARLHTLGRTSAPATELRVSGGGAGLAVWNQIKADVTGIPVVRVAGDSTAAGAAMLAG
                     LGAGVYRDAAEAVSAGYRPLGRAEPDPDHRTLYDDLYERYQALLGSRVVR"
BASE COUNT          203 a          600 c          567 g          204 t
ORIGIN      
        1 aagtcgtcca cctggctggc ccatccgtcc aggtcgtcgc ccacgccgtt gatcaggacc
       61 agggtcggcg ctgctgcctc cccggcgatc ctgtagtgga tgccgatccc gttgctgtct
      121 acgaaaccgc tggtcatcgc gcccctcgct cggtcagtgc ggcctgccat agctcggccg
      181 aggcccgcag gtccgccagc gcctcggcgt cgtcctgctc gaacgccggg atcacctcca
      241 gcaccaggag aacgtcctgc gccggcctcg gccagtgtgt ccagcacgcg gccggggtcg
      301 atgcggccgg cctggttccg ctcggcggtg aacggccagt ggtggtcggc gaggccgtcg
      361 gactgctgca gctggacctc ggcgagccgg ccgccgaagt gcgtgagcca cgcgtacgga
      421 tcccggtcgg gcccggtggt gccgggtacg cactggtgcc cgacgtccag gcagagccga
      481 atcgacgcgt gggcggcgtc gccgtcggtg agcagggtct ccaggccggc catggtcgac
      541 ggctcgcggt gacaggcgag gttttccacc agcaggtagt cgagtcccgc cgagcgggcc
      601 gcggccgcga tcgcggcgag cgagtgctgc agcccggccc agcgctcggc ccggcgcgcc
      661 tcgtcggccc agtcgggcac gctcatcgtg ccgacgtgac cgcccgcccc gctcgccccg
      721 agccgcgcgg ccaggtcgac gacgcccagg taccactcct cggcggcgcg ccgccgctcc
      781 tcgccggggt gcatcagcag gttgagcgag taggcggcca ggccggtgaa cacggtttcc
      841 gcccgtagcc cgtattcatc gagggccgaa cggacctggt taaccgctcc cccgccgaag
      901 tcctcgatca ggtcgaggga aagctccacg acctcgaggc cgagctcgtc gcgcacgatc
      961 cgggcccagt cctccggccg cggccagcgt ttgaccgcga agcaggtgtt gatgcccagg
     1021 cgcgctctca cctgaccacc cgcgatccga gcagggcctg gtagcgctcg tacaagtcgt
     1081 cgtacagggt gcggtgatcg gggtcgggtt cggcccggcc gagcgggcgg tagccggccg
     1141 agaccgcctc ggcggcgtca cggtagaccc cggcgcccag gcccgcgagc atcgcggccc
     1201 ccgccgcggt ggagtcaccc gccacccgca cgaccgggat cccggtgacg tccgccttga
     1261 tctggttcca gaccgccagg cccgcgcccc cgccggacac ccggagctcg gtggccggcg
     1321 cggatgtccg gcccaaggta tggagccggg cccggacgcc gcaggcgatc ccctccagcg
     1381 cggcgcgggc cacggcgccg cgatcgtgcc gcagggacag cccggtgacc gcgccgcgca
     1441 gcgccgggtc gtcccgctcg ccgtcgccga gcacgggcgc gaacaccagg ccgcccgcgc
     1501 cccgcgggat tcggccacgg cctgctcaag ccgggcgtag tcggccgggc gggcccgggc
     1561 gtagaacagc tcca
//
LOCUS       DASPYY010000293         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1177396, whole genome shotgun sequence.
ACCESSION   DASPYY010000293 DASPYY010000000
VERSION     DASPYY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177396"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VEP73_04520"
     CDS             <1..1092
                     /locus_tag="VEP73_04520"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HYT25732.1"
                     /translation="SDTIGNVSRAVGGLTLPNLGRLGLGHLTAIAGVPPDPAAQGFFG
                     KMQERSRGKDTITGHWEMMGAVLTEGLATFPKGFPAELVEAFVHETGAPGVLGNVVAS
                     GTVIIQELGEEHVRTGKPIVYTSADSVFQIATHKRAVPLERLYEWCEAARALLTGEHA
                     VGRVVARPFDGEPGSFHRTKERRDYALPPAGPTILEAVQKAGVRTLGVGKIEDIFSRQ
                     GLDDSDHTGDNQSSLDATARFLHRAEAPTLVFTNLVDFDMLYGHRRDVAGYGRCLEEL
                     DARLPEVVAELGEADWLFLTADHGCDPTAPGTDHTRELAPMVAFSPGGTAGVRLADRD
                     TFADLGATVADLFGVQPVGPGRSFADDLA"
     gene            1206..1553
                     /locus_tag="VEP73_04525"
     CDS             1206..1553
                     /locus_tag="VEP73_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25733.1"
                     /translation="MEGRAGLPGADELFRVTGIDNGRGGRGGDRAGADGAPPAPPAER
                     TSSGRQRHEEKVTFYCTAEELTDLEHARLQLRRQHGIRVDRGRIVRAALAQVLSELER
                     DGAESALVKRLGQ"
     gene            1633..>1891
                     /gene="deoA"
                     /locus_tag="VEP73_04530"
     CDS             1633..>1891
                     /gene="deoA"
                     /locus_tag="VEP73_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075409.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HYT25734.1"
                     /translation="MRVVALIARKRDGGEVPPGELEELVLGYARGDVPDYQMAAFLMA
                     GYLRGFSMAETEALTAAMVASGDRLDLSGLSGPTVDKHSTGG"
BASE COUNT          250 a          711 c          695 g          235 t
ORIGIN      
        1 tccgacacca tcggcaacgt gagccgggca gtgggtgggc tcaccttgcc caacctgggc
       61 cggctcgggc tcgggcacct caccgcgatc gcgggcgtcc cccccgatcc ggcggcgcag
      121 ggcttcttcg ggaagatgca ggagcgctcg cgcggcaagg acaccatcac cggccactgg
      181 gagatgatgg gggcggtcct gaccgaaggg ctcgccacct tcccgaaggg cttcccggcc
      241 gagctcgtcg aggccttcgt ccacgagacg ggcgctcccg gcgtgctcgg caacgtggtc
      301 gcgagcggca ccgtcatcat ccaggagttg ggggaggagc acgtccggac cgggaagccg
      361 atcgtctaca cctcggccga ctcggtcttc cagatcgcca cccacaagcg ggccgtgccg
      421 ctcgagcggc tctacgagtg gtgcgaggcg gcccgcgcgc tcctcaccgg ggagcacgcg
      481 gtcggccggg tcgtcgcccg cccgttcgac ggcgagcccg ggtcgttcca ccggaccaag
      541 gagcggcggg actacgcgct gccgcccgcc ggcccgacca tcctggaggc ggtccagaag
      601 gccggcgtcc gcacgctcgg ggtcggcaag atcgaggaca tcttctcgcg ccagggcctt
      661 gacgactccg accacaccgg cgacaaccag agctcgctcg acgcgaccgc gcgcttcctg
      721 caccgggccg aggcgccgac gctcgtgttc accaacctgg tcgacttcga catgctctac
      781 gggcaccggc gcgacgtggc cgggtacggc cgctgcctgg aggagctcga tgcgcgcctg
      841 cccgaggtgg tcgccgagct gggggaggcc gactggctct tcctgaccgc cgaccacggc
      901 tgcgacccga ccgcgcccgg caccgaccac acccgggagc tggccccgat ggtggcgttc
      961 agccccggcg ggaccgcggg cgtgcgcctg gccgaccggg acacgttcgc cgacctcggg
     1021 gcgaccgtgg ccgacctgtt cggggtccag ccggtcggcc cgggccgctc gttcgccgac
     1081 gacctggcct gaccggcccc ggccccggcc cgcgatccgg ctccgcctga ttcggtacta
     1141 tccgggtcgc tcgatcgatc aggcgactga tggcttcgtc gagctgccaa ggaggtggcg
     1201 gcgtcatgga gggtcgggcc ggcctgccag gggccgacga gctgttccgc gtcaccggca
     1261 tcgacaacgg ccgcggcggc cgtggaggcg accgggccgg cgcggacggg gcgccgcccg
     1321 cgccgcccgc cgagcggacc tccagcggcc gccagcgcca cgaggagaag gtcaccttct
     1381 actgcacggc cgaggagctg accgacctgg agcacgcccg gctgcagctc cgccgccagc
     1441 acggcatccg cgtcgaccgc ggccgcatcg tccgcgcggc gctcgcccag gtcctctccg
     1501 agctggagcg cgacggcgcc gagtcggccc tggtgaagcg cttgggccag tagcccgccg
     1561 tccgccgagg gagaggagcg cccggcccgc ggcgggcgcg tccccaccgt ccgtcgaggg
     1621 agaggagcgc agatgcgggt cgtcgcgctg atcgcgcgca agcgcgacgg gggcgaggtg
     1681 ccgcccgggg agctcgagga gctggtgctc gggtacgccc gcggcgacgt gcccgactac
     1741 cagatggccg ccttcctcat ggccggctac ctgcgcggct tctccatggc cgagaccgag
     1801 gcgctcaccg ccgcgatggt cgcctccggc gaccggctcg acctgtccgg cctgtccggg
     1861 ccgaccgtcg acaagcactc gacgggcggg g
//
LOCUS       DASPYY010000294         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1597690, whole genome shotgun sequence.
ACCESSION   DASPYY010000294 DASPYY010000000
VERSION     DASPYY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597690"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VEP73_04535"
     CDS             complement(<1..255)
                     /locus_tag="VEP73_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25735.1"
                     /translation="MLVDADNLDAGRLRLVAAELSGVRRLVVAGHPSRLATVRWPDAA
                     DVRPASGWQSADSVLAAAYEPDEEPLVLVTGDGDFALLAAR"
     gene            complement(303..1565)
                     /locus_tag="VEP73_04540"
     CDS             complement(303..1565)
                     /locus_tag="VEP73_04540"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HYT25736.1"
                     /translation="MSTAEVVAPTTVRTLPVDGLLAGMPAALLERLPADAPLAWVRGG
                     DGLVGWGEAARVELTGPDRFAAAELWWERWLDGVVVDDPLTVPGTGPVAFASFSFDQD
                     AGSSVLVVPRVLLGRRGGTTWVTVVGDGPDMASADLPPVSVPMSPVEVRWSQGSLSTV
                     QWQGAVRQAVARIVRGDLDKVVLARDLVATASAPVDVRYLLGRLADRYPSCWTFSVDG
                     LVGATPELLVRRTGDLVLSRVLAGTVGRLGDDAADGELADALLGSEKDLEEHEFAVHS
                     VAAVLAGYCAELDVPEQPKVLRLRNVQHLATDVTGRLTDGSGVLELAGALHPTAAVCG
                     TPTDVALQVIRELEGMDRGRYAGPVGWLGRDGDGEFGLALRCAEVSGERLRLFAGCGI
                     VAGSDAEAELTESQAKFVPIRDALESEG"
BASE COUNT          249 a          645 c          592 g          184 t
ORIGIN      
        1 tcgcgccgcg agcagggcga agtcgccgtc gccggtcacc agcaccaacg gctcctcgtc
       61 gggttcgtac gcagcagcca ggactgagtc ggcgctctgc caaccgctgg cggggcgtac
      121 gtccgcggcg tccggccatc gcaccgtggc gagccgggac ggatggccgg cgacgacgag
      181 ccggcgcacg ccggacagct ctgcggcgac caggcgaagc cggccggcat ccaggttgtc
      241 cgcgtcgacg agcacctgtg gtccggcacc cggcggccgg gccttcggac ggtcgtcgac
      301 cgtcagccct ccgactccag cgcgtcccgg atcgggacga acttcgcctg cgactcggtg
      361 agctcggcct cggcgtccga gccggcgacg atgccgcagc ccgcgaacag cctgagccgc
      421 tcgccggaca cctcggcgca gcgcaacgcc aggccgaact caccgtcccc gtcccggccg
      481 agccatccga ccgggccggc gtaccgacca cggtccatgc cctcgagctc gcggatcacc
      541 tggagcgcga cgtccgtagg ggtgccgcac acggccgcgg tcgggtgcag ggccccggcc
      601 agctcgagca cgccgctgcc atcggtgagg cggccggtca cgtccgtcgc gaggtgctgg
      661 acgttgcgca gccgcagcac cttgggctgc tcggggacgt cgagctcggc gcagtagccg
      721 gcgagcacgg cggcgacgga gtgaacggcg aactcgtgct cctcgaggtc cttctccgaa
      781 ccgagcagcg cgtcggcgag ctcgccgtcg gcggcgtcgt cgccgagccg gccgaccgtg
      841 cctgccagca cccgcgagag caccaggtca ccggtccgtc gcaccaggag ctccggggtg
      901 gcccccacca ggccgtcgac cgagaacgtc cagcaggagg ggtatcggtc ggccagccgc
      961 cccaacaggt accgcacgtc gaccggggca gacgccgtcg ccaccaggtc ccgggccagc
     1021 acgaccttgt cgaggtcgcc gcggacgatc cgcgcgaccg cctggcgtac agccccctgc
     1081 cactgaacgg tgctcagcga tccctgcgac cagcgcacct cgacgggcga catgggcacc
     1141 gagaccggcg ggaggtcggc cgaggccata tccgggccgt cgccgacgac cgtcacccat
     1201 gtcgttccgc cgcgtcgtcc gaggagcacc cgcggcacca ccaggaccga ggagcccgcg
     1261 tcctggtcga acgagaacga cgcgaaggcc actggacccg ttccgggcac tgtcagcggg
     1321 tcgtcgacga cgacaccgtc gagccaccgc tcccaccaga gctcggcggc cgcgaaacgg
     1381 tccgggccgg tcagctcgac ccgtgcggct tcgccccaac cgacgaggcc gtcgccgccg
     1441 cgcacccacg ccagcggcgc atcggcgggg agccgctcca gcagcgcagc cggcatgccg
     1501 gcgagcaggc cgtcgacggg gagggtgcgc actgtcgtgg gcgcgaccac ttctgcggtg
     1561 gacacaacgc accagggtag gccccgcacc ggggccgggc agccggaccg gtccgaacga
     1621 gcccggatcc cgttccacgt ctgcgggcgc acggtgccgc cgactgccta
//
LOCUS       DASPYY010000295         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_333072, whole genome shotgun sequence.
ACCESSION   DASPYY010000295 DASPYY010000000
VERSION     DASPYY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333072"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /locus_tag="VEP73_04545"
     CDS             <1..>1706
                     /locus_tag="VEP73_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25737.1"
                     /translation="RPPRRLGWLRSPALLALLAAVALSAWVFRAAWAAPSAAAIGVHG
                     DPWLFAWFLKWDQFALAHGHDPLVSRYLGVPDGVNLMWNTSVLLPGVLLAPVTAAFGP
                     VLTYNLLVTLGVALSAWCAYLALRRYVDSDLAAGAGGLLYGFSPYMLGHAYGHLHLIL
                     AFGPPLMLALLDEILVRQRAAPVKAGALLGVLAAVQLLVAEELLASELLVATMALALL
                     VVLHARRVRAHAGHAARALGVAAAVFAVLVAVPLAVQFLGPQHPHGTIQTPGVAVTDL
                     ANLVVPTRLQRFAPARALSISNHFTSNPTEWDGYLGLPLLLLLAGTTVSLWRRPLVRF
                     ASVLALGIVVLSLGPRLHVGGHVTRVHLPWRAVEALPVLDNMLPNRLMLYVYLLAGLL
                     VALFADAALRSRDGRRVALATATLVIALVPLVPRLPFPATAAGTPGFFTSGDVRRVPV
                     GSVALVAPFPRYPPTVDPMLWQATAGMRYRMAGGYFVGTDRTGRAIFGVVGTTLSQSI
                     EAVQNGGRPPTLTRTMRARLLGTLRAWHVRTVIVGPMRHQAALLAFYALLLGRPPERV
                     DGV"
BASE COUNT          155 a          703 c          573 g          275 t
ORIGIN      
        1 cggccacccc ggcggctcgg ctggctgcgg tcgcccgcgt tgctcgcgct cctcgccgcg
       61 gtcgcgctct cggcatgggt gttccgggcg gcgtgggcgg ctccgagcgc cgccgccatc
      121 ggggtgcacg gcgacccctg gctgttcgcc tggttcctga aatgggacca gttcgcgctc
      181 gcccacgggc acgacccgct ggtgagccgc tacctcggcg tccccgacgg cgtgaacctc
      241 atgtggaaca cgtcggtcct gctccctggg gtgctgctgg cgcccgtcac ggccgcgttc
      301 gggccggtgc tcacctacaa cctcctcgtc acgctcgggg tcgcgctgtc ggcgtggtgc
      361 gcctacctgg cgctgcgccg gtacgtggac tcggatctcg ccgccggcgc cggcggcctc
      421 ctctacggct tctcgccgta catgctcggc cacgcctacg gtcacctcca cctgatcctc
      481 gccttcggcc cgccgctcat gctcgcgctg ctggacgaga tcctcgtccg ccagcgcgcc
      541 gccccggtca aggcgggcgc gctgctcggc gtgctcgccg ccgtccagct cctggtcgcc
      601 gaggagctgc tcgcctccga gctcctcgtc gccaccatgg cgctcgcgct cctcgtcgtc
      661 ctgcacgctc ggcgggtacg ggcccacgcc ggccacgccg cccgggcgct cggcgtcgcg
      721 gcggccgtgt tcgccgtcct ggtggcggtg ccgctggccg tgcagttcct cgggccacag
      781 catccccacg ggaccatcca gacgccgggc gtcgccgtca ccgacctggc gaacctcgtc
      841 gtcccgaccc gcctgcaacg gttcgcgccg gcgcgtgccc tgtcgatctc caaccacttc
      901 acgagcaacc ccaccgagtg ggacggctac ctcggcctgc ccttgctgct gctgctcgcg
      961 gggaccacag tcagcctctg gcggcgaccg ctcgtgcggt tcgcgagcgt gctcgcgctc
     1021 gggatcgtcg tcctgtcgct cgggccgcgg ctgcacgtcg gcgggcacgt cacgcgcgtg
     1081 cacctgccct ggcgggcggt cgaggcgctg cccgtgctcg acaacatgct gccgaaccgg
     1141 ctgatgctct acgtgtacct gctcgcgggg ctgctcgtcg ccctgttcgc cgacgcggcg
     1201 ctgcgctccc gggacggccg gcgtgtcgcc ctcgccaccg ccacgctggt gatcgccctg
     1261 gtcccgctcg tcccccgcct gccgttcccg gccaccgccg ccggcacgcc cgggttcttc
     1321 acctccgggg acgtgcgccg cgtcccggtg ggcagcgtcg cactcgtcgc gcccttcccg
     1381 cgctacccgc ccaccgtcga cccgatgctc tggcaggcga cggcgggcat gcgctaccgg
     1441 atggccggcg gctacttcgt cggcacggac cggaccggcc gggcgatctt cggcgtggtc
     1501 ggcaccaccc tgtcccagag catcgaggcg gtccagaacg gcggccggcc gcccacgctg
     1561 acccgcacca tgcgcgcccg gctgctcggc accctgcgcg cctggcacgt ccggacggtg
     1621 atcgtcggcc ccatgcgcca ccaggccgcc ctgctggcgt tctacgccct gctcctcggc
     1681 cggccgccgg agcgcgtcga cggcgt
//
LOCUS       DASPYY010000296         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_475487, whole genome shotgun sequence.
ACCESSION   DASPYY010000296 DASPYY010000000
VERSION     DASPYY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475487"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..379
                     /locus_tag="VEP73_04550"
     CDS             128..379
                     /locus_tag="VEP73_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25738.1"
                     /translation="MRTARGRSTVLLAAAAWTVYVWVTRIWNILRDPAHGAGFKAVHG
                     VLAAISIAFAVAVGVIGLRMRREARAERPGPAEREPAGR"
     gene            586..1164
                     /locus_tag="VEP73_04555"
     CDS             586..1164
                     /locus_tag="VEP73_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25739.1"
                     /translation="MQAELEHWDGDLGAGAPGWRGLTGGVTDDGELVVILRFDGAGAA
                     VDNDTRPATAVWRTAVERHLAGPARLQRCPIVREIKGGGSDEAGFVRVLQGRVIDARR
                     LAALQDDVERGLAEEAHVLGVTVADHDRGGWFTEVTYFTSERAVRAAERAMPVAKAVQ
                     LGMVRSYMEGLRLYELRAPCLASPRPQAVPAG"
     gene            complement(1198..2430)
                     /gene="larC"
                     /locus_tag="VEP73_04560"
     CDS             complement(1198..2430)
                     /gene="larC"
                     /locus_tag="VEP73_04560"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYT25740.1"
                     /translation="MTTSCSFAYFDCFAGIAGDMALGALLDAGGSPAALWAGLEGLAP
                     ALDPFQLEIATVDRGGIGATRVKVHTDHSSVVRTWGNLRAILGEADLPEPVRARAMAV
                     FERLAEAEGRVHRKPPDQVHFHEVGAVDAVVDVVGTALLLHDLGVREIWASAVATGSG
                     RTRGEHGVLPVPGPAVLELLRGAPVYSGGVAAELTTPTGAAILAAGAARYGDLPAMRV
                     ARIGYGAGSRQHDELPNVLRVVLGELAEDPVAGDGGVVLEANIDDMSPELAPWVFERL
                     LSAGAADVWLTPIHMKKGRPGITLSVLCPPGADARVRELLWRETSTLGVRGVPVRKWM
                     LERRTVEVRLLGGTVRVKLGLDGGRVVNVAPEFADCARLAAQADRPLKEVMARAQALA
                     LADLDAPPGIGPAGPDPG"
     gene            2556..3077
                     /locus_tag="VEP73_04565"
     CDS             2556..3077
                     /locus_tag="VEP73_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HYT25741.1"
                     /translation="MPALSVESLTLLVAVALALCGALAVTVTWLAVRLHGIHRTYARL
                     RTGGSKDEDVLTVIAHQMAAIDRLEGRLDLVGRQAAEHRQHLSTLVRSVGFHRYDAFP
                     DTGGQLSYSAAFLDEAGDGIVLTAINGRSETRSYAKRVKGGTSAHNLSDEERTAVAMA
                     MGATSRPVTIRLS"
     gene            3232..4497
                     /gene="serS"
                     /locus_tag="VEP73_04570"
     CDS             3232..4497
                     /gene="serS"
                     /locus_tag="VEP73_04570"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYT25742.1"
                     /translation="MLDLKLVRDHPARVRAALARRGPGVADAVDRLLELDASRRALVT
                     EVDALRSEQKRRGKDVAAVAPDERPGLLAGLKGLAARIDEVEARLRAVEAELADTLAR
                     VPNLPDPSVPGGGEDDAVELRRSGEPPAFGFEVRDHLELGELLGAIDTVRAAKVSGAR
                     FGYLTGPGAMLELALVRFAMDRLTGAGFTPMIPPVLVRREAMYGTGFLPTDEQQIFRT
                     ADDDLYLVGTSEVPLVGYHADEILDPGTLPRRYAGFSSCFRREAGSYGKDTRGIFRVH
                     QFDKVEMVSFVLPEASWEEHEFLLAREEEILRALELPYRVVNIAAGDLGGSAAKKYDC
                     EAWIPSQGTWRELTSTSNCTDYQARRIGARVREAEGIRPLHTLNGTAVAVGRTIIALL
                     ENHQQPDGSVRVPAALQQYLGTDLIPVAK"
     gene            4507..4591
                     /locus_tag="VEP73_04575"
     tRNA            4507..4591
                     /locus_tag="VEP73_04575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4541..4543,aa:Ser,seq:tga)
BASE COUNT          624 a         1800 c         1695 g          610 t
ORIGIN      
        1 cgcgggatgc gcctgtacga ggggaccagc gagatcctca aggccaacgt cgcccgcacc
       61 ctcggcctgc cggcgtgacc ggcgcccgaa ggccgccgtt ccccgcgcgg cccggtagga
      121 tggcccgatg agaaccgctc gaggccgctc gaccgtcctg ctcgccgcgg ccgcctggac
      181 ggtctatgtc tgggtgaccc ggatctggaa catcctgcgc gaccccgcgc atggcgccgg
      241 gttcaaggcc gtgcatggcg tgctggcggc gatctcgatc gcgttcgcgg tcgccgtcgg
      301 cgtcatcggc ctgcgcatgc gccgcgaggc cagggcggag cggcccggcc cggccgagcg
      361 cgagcccgcc ggccgctgag cgtcgccgcc cacccggtgg caggcaccca ccgcccgcac
      421 ccgtcgtggg cgaccgtccc ggcgcgagct gtcctgtcta ccggaacgtc ctacttgact
      481 tgacagccac gacgagtagc ttgagccgga caatctggtc gccggggcgg ggggttcggc
      541 atgttcatcc aggtcatgca ggggcgagtc cgggatcgca ccggtgtgca ggcggagctc
      601 gaacactggg acggggatct cggcgcgggc gcgcctgggt ggcgcggcct caccggcggg
      661 gtcaccgacg acggtgagct cgtcgtcatc ctccggttcg acggcgcggg ggccgcggtc
      721 gacaacgaca cgaggccggc gacggccgtc tggcggaccg cggtggagcg ccatctcgcc
      781 ggcccggcgc gcctgcagcg gtgcccgatc gtccgcgaga tcaagggcgg cggctccgac
      841 gaggccgggt tcgtccgcgt cctgcagggc cgggtcatcg acgcgaggcg cctcgccgcc
      901 ctgcaggacg acgtcgagcg cgggctcgcg gaagaggcgc acgtcctcgg cgtgaccgtc
      961 gccgaccacg accggggcgg ctggttcacc gaggtcacct acttcacctc cgagcgggcg
     1021 gtccgtgcgg ccgagcgggc gatgccggtc gccaaggccg tgcagctcgg catggtgcgc
     1081 agctacatgg agggcctgcg gctctacgag ctgcgcgccc cgtgcctcgc gagcccgcgc
     1141 ccgcaggccg tcccggcagg ctgaccgcca gggtccacgc ccgccggccc ggccgggtca
     1201 gcccgggtct ggcccggccg ggccgatgcc aggcggcgcg tccaggtcgg ccagtgccag
     1261 cgcctgcgca cgggccatga cctccttcag cggccggtcc gcctgggcag ccaggcgggc
     1321 gcagtcggcg aactccgggg cgacgttcac cacccggccg ccgtcgagcc cgagcttgac
     1381 ccgtaccgtg ccgccgagca gcctgacctc gaccgtccgg cgctccagca tccacttccg
     1441 cacgggcacg ccgcgcacgc cgagcgtcga ggtctcccgc cacaggagct cgcgcacgcg
     1501 cgcgtcggcg cccggggggc agagcacgga cagggtgatg ccgggccggc ccttcttcat
     1561 gtggatcggg gtgagccaca cgtcggccgc cccggccgag agcagtcgct cgaacaccca
     1621 gggggccagc tcggggctca tgtcgtcgat gttcgcctcg agcacgaccc cgccgtcgcc
     1681 cgccacgggg tcctccgcca gctccccgag caccacccgg aggacgttcg gcagctcgtc
     1741 gtgctggcgg ctgcccgcgc cgtagccgat acgggcgacc cgcatcgccg ggaggtcgcc
     1801 gtagcgggcg gccccggctg ccaggatggc cgcgcccgtc ggggtcgtga gctccgcggc
     1861 gacgccgccc gagtagaccg gcgctccgcg cagcagctcg aggacggccg ggccgggcac
     1921 gggcagcacc ccgtgctcgc cgcgcgtgcg cccgctcccg gtggccaccg ccgacgccca
     1981 gatctcgcgg accccgaggt cgtgcaggag cagcgcggtg ccgaccacgt cgaccaccgc
     2041 gtccacggcg cccacctcgt ggaagtgcac ctggtcgggc ggcttgcgat ggacccgccc
     2101 ctcggcctcg gccagccgct cgaacacggc catggcgcgg gcccgcaccg gctcgggcag
     2161 gtcggcctcg cccaggatgg cccggaggtt gccccaggta cgcaccacgc tcgagtggtc
     2221 ggtgtgcacc ttgaccctgg tcgcgccgat gccgccgcga tcgaccgtgg cgatctcgag
     2281 ctggaagggg tcgagcgccg gggccagtcc ctcgagcccc gcccacagcg ctgccgggga
     2341 gcctccggcg tcgagcaggg cgccgagcgc catgtcgccg gcgatacccg cgaagcagtc
     2401 gaagtaggcg aacgagcaag atgtggtcat cgatgcgcct cgggccgggg cgctcgagga
     2461 acgctcgacg ccgcgttgat gcaacgacct ggcattgtac aatcgcgccg ctctccccaa
     2521 ccgccacctt cccccaaccc tggaggtacc gagccgtgcc cgcgctgtcg gtcgagagcc
     2581 tgaccctcct ggtcgccgtc gcgctcgccc tctgcggggc gctcgccgtc acggtgacct
     2641 ggctcgccgt gcgcctgcac gggatccacc gcacctacgc ccggctgcgc accggggggt
     2701 cgaaggacga ggacgtcctc accgtgatcg cccaccagat ggccgcgatc gaccgtctcg
     2761 agggcaggct cgacctcgtc ggacggcagg cggccgagca ccgccagcac ctgtccaccc
     2821 tcgtccggtc ggtcggtttc caccgctacg acgcgttccc cgacacgggt ggccagctct
     2881 cctactcggc cgccttcctg gacgaggccg gggacgggat cgtgctgacg gccatcaacg
     2941 gccggtccga gacgcgctcc tacgccaagc gggtcaaggg cggcacatcg gcccacaacc
     3001 tctccgacga ggagcggacc gcggtcgcga tggccatggg cgcgacctcc cggccggtca
     3061 cgatccgcct ctcgtagccg cagtccccgg gtgccgcggg gtgggctcga ccctcggcga
     3121 ccgggggaga gatatcctgt cgaccgcgcg cacccgcagc gcgatgatcg ctcggccgga
     3181 cgctcgacgg cgtcggcccg gcccatgtcc cggtatccgg aggaacccag ggtgctcgat
     3241 ctcaagctcg ttcgtgacca ccccgcccgc gtgcgggcgg ctctggccag gcgcggccct
     3301 ggcgtggccg acgcggtgga ccgtctgctg gagctggacg ccagccggcg tgccctggtc
     3361 accgaggtcg acgccctgcg gtcggagcag aagcgccggg gcaaggacgt cgccgcggtc
     3421 gcgccggacg agcgtcccgg gctcctggcc gggctgaagg gcctggccgc gcgcatcgac
     3481 gaagtggagg cccgcctgcg cgccgtcgag gccgagctgg ccgacacgct ggcccgcgtg
     3541 ccgaacctgc ccgacccgtc ggtgcccggc gggggagagg acgacgcggt cgagctgcgg
     3601 cggtccggcg agccgcccgc cttcggcttc gaggtacgcg accacctcga gctcggcgag
     3661 ctgctcggcg ccatcgacac cgtccgggcg gccaaggtct ccggggcccg cttcggctac
     3721 ctcaccggcc cgggggcgat gctcgagctc gcgctcgtgc gcttcgccat ggaccgcctc
     3781 accggggccg gcttcacgcc catgatcccg cctgtcctcg tccggcggga ggcgatgtac
     3841 gggacggggt tcctgccaac cgacgagcag cagatcttcc gcacagccga cgacgacctc
     3901 tacctcgtcg gcacgtcgga ggtgccgctg gtcggctacc acgccgacga gatcctcgac
     3961 cccggcaccc tgccccgccg gtatgccggc ttctcgtcgt gcttccgccg cgaggcgggc
     4021 agctacggca aggacacgcg tggcatcttc cgcgtccacc agttcgacaa ggtcgagatg
     4081 gtctccttcg tcctgcccga ggcgtcctgg gaggagcacg agttcctgct cgcccgcgag
     4141 gaggagatcc tgcgcgccct cgagctgccc taccgggtgg tgaacatcgc agcgggcgac
     4201 ctcggcggct ccgcggccaa gaagtacgac tgcgaggcgt ggatcccgtc gcaggggacc
     4261 tggcgcgagc tcacctcgac ctcgaactgc accgactacc aggcgaggcg gatcggcgcc
     4321 cgggtcaggg aggccgaggg catccggccc ctccacacgc tgaacggcac cgccgtggcg
     4381 gtcggccgca ccatcatcgc cctgctcgag aaccaccagc agcccgacgg ctcggtgcgg
     4441 gtccccgccg ccctccaaca gtacctgggc accgacctca tcccggtcgc caagtagact
     4501 cccggcggag gagtggccga gcggtcgaag gcagcgacct tgaaagtcgc cggaccccag
     4561 ggtcccgtgg gttcgaatcc cacctcctcc gccttcgcca gccccaaatc agcagcgctg
     4621 ctacgattct gatcgtgagc gcgtgcggag gagagcccgg ccggtggcac ccgaccggac
     4681 ggcccttgcc tcggggctga gcggcctggc cgtggccgcg attgcggcc
//
LOCUS       DASPYY010000297         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_615190, whole genome shotgun sequence.
ACCESSION   DASPYY010000297 DASPYY010000000
VERSION     DASPYY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615190"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VEP73_04580"
     CDS             complement(<1..556)
                     /locus_tag="VEP73_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25743.1"
                     /translation="MPGFAAALAELGDAELGRMLARRPDLLAGRGPASFPELASRAGT
                     PASMAAALRQLDLGTLQLAELLAVVGLPTTVGSLAEAAGPGLRPERLRAGLAALAELG
                     LALPAPDGGIAGPRGLAAPFGSPGGLGPSVAELAKIGVTRDQLDLVAGNLGIERPPAK
                     QALVAAVAAALADPSVVGKVAGGAD"
     gene            complement(706..1521)
                     /locus_tag="VEP73_04585"
     CDS             complement(706..1521)
                     /locus_tag="VEP73_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT25744.1"
                     /translation="MERRVLGFSGLRVPVVGMGTSRTFDVRGRAAETTSRKIVDEALA
                     VGANLFDTSPMYGEAERVLGMALEGRRSEALVATKVWTASDEEAERQARASLRYAHGH
                     VDLYQVHNLVAWPARLDLLERLRDEGLVTAVGATHYDPGAFDELATVMRTGRVSAIQV
                     PYNPLEPEVERTVLPLAEELGLGVVLMRPFGRGRVLRGTWPTAAQLAPFRPFGVVTWP
                     QVLLKWGLSDPRCTVSIPATSKPGRMTENAAAGEPPWFGPEERDLAGRLARRL"
     gene            complement(1679..>2089)
                     /locus_tag="VEP73_04590"
     CDS             complement(1679..>2089)
                     /locus_tag="VEP73_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25745.1"
                     /translation="ALLLFGEAIGVRFAGNEIVGALGLLGLTVLFGLGFSAISIAVAL
                     ATGSVRATQTTFVVFFPLLFLTPGTLPRNLMTGWFKTAVAFNPITYVLEAARSLIFGT
                     STSVIAKGVLACLAFCAFTFALTFLAYRRRVRMA"
BASE COUNT          285 a          759 c          815 g          230 t
ORIGIN      
        1 cgtcggcccc gccggccacc ttgccgacca ccgaggggtc ggcaagggcg gcggcgacgg
       61 ccgcgaccag ggcctgcttg gccgggggtc gctcgatgcc gaggttcccg gcgacgaggt
      121 cgagctggtc cctggtgacc ccgatcttgg ccagctcggc gaccgagggg ccgaggccgc
      181 ccgggctccc gaagggcgcg gccagcccgc gcgggcccgc gatgccgccg tcgggagccg
      241 ggagggcgag cccgagctcg gccagggcgg ccaggccggc gcgcaggcgc tcggggcgca
      301 ggccggggcc ggcggcctcg gcgagcgagc cgacggtggt cggcagcccg acgaccgcca
      361 gcagctcggc gagctgcagc gtgccgaggt cgagctggcg cagcgcggcc gccatcgacg
      421 ccggcgtgcc ggcccggctg gccagctcgg ggaacgacgc cggtccccgc ccggccagga
      481 ggtccggccg gcgtgccagc atcctgccga gctcggcgtc gccgagctcg gccagggcgg
      541 ctgcgaaacc gggcacggct gtcctccttc gttgcctggg tgggggtcgg gccctggccc
      601 gtgccgccgg ggcgccacgg acgttcaggc gagggtaccg cacgtcgggg tggcggccga
      661 cccggctggt gcggcggccg ctcccgcacc atcacgggat ggccgtcaga ggcggcgggc
      721 gaggcggccg gcgaggtcgc gctcctcggg gccgaaccac ggcggctccc ctgccgccgc
      781 gttctcggtc atccgccccg gcttggaggt ggccgggatc gagaccgtgc agcgggggtc
      841 ggacagcccc cacttgagga gcacctgcgg ccaggtgacc acgccgaacg gccggaaggg
      901 cgcgagctgg gccgcggtcg gccaggtgcc ccgcaggacc ctcccccggc cgaacggccg
      961 catcagcacg acgcccaggc cgagctcctc ggcgagcggc agcacggtcc gctccacctc
     1021 gggctcgagc gggttgtagg gcacctggat cgcgctgacg cggccggtcc gcatgacggt
     1081 ggcgagctcg tcgaaggcgc cggggtcgta gtgggtggcg ccgacggcgg tgacgagccc
     1141 ctcgtcgcgc agccgctcca gcaggtcgag gcgggccggc caggccacca ggttgtggac
     1201 ctggtagagg tcgacgtgcc cgtgcgcgta gcggagcgag gcacgggcct ggcgctcggc
     1261 ctcctcgtcg ctcgcggtcc acaccttggt ggcgaccagc gcctcggacc gccggccctc
     1321 gagggccatg ccgaggaccc gctcggcctc gccgtacatg ggcgaggtgt cgaagaggtt
     1381 cgcgcccacc gcgagcgcct catcgacgat cttgcggctg gtggtctcgg cggcccttcc
     1441 ccggacgtcg aacgtgcgcg acgtccccat gccgaccact ggcacccgca gcccgctgaa
     1501 ccccaggacc cgtcgttcca cgccccgcgc cccctccccc tcacgcccgc ccggccccga
     1561 cgggcaccgg accggagcgc gcgagggctt caacgaccga ccaagggtac ggggacggca
     1621 gggcgatgac taccggtgga tcaccgttcg agacgatcgg cgtgagcgtc ccgccgcgct
     1681 aggccatccg cacccggcgg cgataggcga ggaacgtgag cgcgaacgtg aaggcgcaga
     1741 acgcgaggca ggcgaggacg cccttggcga tcacgctcgt gctggtgccg aagatcaggg
     1801 accgggccgc ctcgagcacg taggtgatcg ggttgaaggc gaccgcggtc ttgaaccatc
     1861 cggtcatgag gttccggggc agcgtgcccg gggtgaggaa cagcagcggg aagaagacga
     1921 cgaaggtggt ctgggtggcc cgcaccgacc cggttgccag ggcgaccgcg atgctgatgg
     1981 ccgagaagcc gagcccgaac agcaccgtca gcccgaggag cccaagggcg ccgacgatct
     2041 cgttgccggc gaagcgcacg ccgatcgcct cgccgaagag cagcagcgc
//
LOCUS       DASPYY010000298         1992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_475756, whole genome shotgun sequence.
ACCESSION   DASPYY010000298 DASPYY010000000
VERSION     DASPYY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475756"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>1992
                     /locus_tag="VEP73_04595"
     CDS             209..>1992
                     /locus_tag="VEP73_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYT25746.1"
                     /translation="MPAPIEDYAIIGDTHTAALVSRSGSIDWLCLPRFDSGACFAALL
                     GNERHGRWLLAPAGEVTRVRRRYRGHSLVLETEFQTPDGVVRVVDCMPPRQGDPDVAR
                     VVEGVRGRVPMRMELIVRFDYGSIVPWVRHTEEGLHAVGGPDSVWLRTPVRVRGRNFT
                     SVAEFTVAEGEKVPFMLTWHWSHKPAPRAIDPVRAVRQTESWWRGWVDQLSYEGPWRD
                     AVVRSLATLKALTYSPTGGIVAAPTTSLPEALGGVRNWDYRYCWLRDATFTLAALMMA
                     GAREEAEAWREWLLRAAAGSPEQMQILYGAAGERRIDELELGWLPGYEGSRPVRTGNA
                     AVNQFQLDVYGEVMDALYLARVAGIPAAVSSWPLERALMDFLESSWQQPDEGIWEVRG
                     PRRHFTHSKVMAWVAVDRAVKAIEFYGTRGPLARWRALRRQLHEEVLRNGFDAERNAF
                     VQYYGAKHLDASLLMIPLVGFLPATDPRMRGTVAAIERELMQDGFVLRYPAEVAEGVD
                     GLPGGEGAFLACSFWLADNLTLAGRHDEARDLFERLLDLRNDVGLLAEEYDPKTGRQV
                     GNFPQAFSHVALVNTAHHLEAGRSLRIPR"
BASE COUNT          255 a          754 c          714 g          269 t
ORIGIN      
        1 gggcgggctg gtcgcccgcc ggccgggggg tcggcatcgg gcggtctgcg gccacggctc
       61 gtcctcacgg gaggtggagg cggccccagt gtacccacag gcccagggcc gcccacccgg
      121 ggagcgggac gtttgccata ctgcaagtcg agccagcccc taccctgccc cggacggcgc
      181 cctgccccga cggggacgag gaccagcgat gccagcaccc atcgaggact acgcgatcat
      241 cggggacacc cacaccgcgg ccctggtgag ccgctccggc tcgatcgact ggctctgcct
      301 gccccgcttc gactccgggg cctgcttcgc cgccctgctc ggcaacgagc gtcacgggcg
      361 ctggctgctc gcccccgccg gcgaggtcac ccgggtccgc cgccgctacc ggggccacag
      421 cctggtgctc gagaccgagt tccagacccc ggacggggtg gtcagggtgg tcgactgcat
      481 gccgccccgc cagggcgacc ccgacgtcgc ccgcgtggtc gagggcgtcc gcggccgggt
      541 gccgatgcgc atggagctca tcgtccgttt cgactacggc tcgatcgtcc cctgggtgcg
      601 ccacaccgag gagggcctgc acgccgtggg cgggcccgac tcggtctggc tgcgcacccc
      661 ggtccgggtg cgcgggcgca acttcaccag cgtggccgag ttcaccgtgg ccgagggcga
      721 gaaggtgccg ttcatgctca cctggcactg gtcgcacaag ccagcccccc gcgccatcga
      781 cccggtgcgg gcggtccgcc agaccgagtc ctggtggcgc ggctgggtgg accagctcag
      841 ctacgagggc ccctggcggg acgccgtggt ccgctcgctg gccaccctga aggcgctcac
      901 ctactcgccc accgggggca tcgtcgccgc gcccaccacc tcgctgcccg aggcgctcgg
      961 cggggtccgc aactgggact accggtactg ctggctgcgg gacgcgacct tcaccctggc
     1021 cgcgctgatg atggccgggg ccagggagga ggccgaggcg tggcgcgagt ggctgctgcg
     1081 ggccgccgcc gggtcgcccg agcagatgca gatcctctac ggggccgccg gcgagcggcg
     1141 catcgacgag ctcgagctcg gctggctgcc cggctacgag ggctcccgcc cggtccggac
     1201 cggcaacgcg gccgtcaacc agttccagct cgacgtctac ggcgaggtca tggacgcgct
     1261 ctacctcgcc cgcgtcgccg ggatcccggc ggcggtctcc tcctggccgc tcgagcgcgc
     1321 cctgatggac ttcctcgagt cgagctggca gcagcccgac gaggggatct gggaggtgag
     1381 gggcccgcgc cggcacttca cccactccaa ggtcatggcc tgggtggcgg tcgaccgggc
     1441 ggtcaaggcg atcgagttct acggcacccg gggcccgctc gcgcgctgga gggcgctgcg
     1501 ccgccagctc cacgaggagg tcctccgcaa cggcttcgac gcggagcgca acgccttcgt
     1561 gcagtactac ggggccaagc acctcgacgc cagcctgctc atgatcccgc tggtcggctt
     1621 cctgcccgcg accgacccgc gcatgcgcgg cacggtcgcc gccatcgagc gcgagctgat
     1681 gcaggacggg ttcgtgctcc gctacccggc cgaggtggcc gagggcgtcg acggcctgcc
     1741 aggcggcgag ggcgcctttc tggcctgcag cttctggctg gccgacaacc tgaccctcgc
     1801 cggccggcac gacgaggcgc gggacctgtt cgagcgcctg ctcgacctgc gcaacgacgt
     1861 cggcctgctc gccgaggagt acgacccgaa gaccggccgg caggtcggca acttccccca
     1921 ggcgttctcc cacgtggccc tggtcaacac cgcccatcac ctcgaagcgg ggcggtcctt
     1981 gcgcatcccg cg
//
LOCUS       DASPYY010000299         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_52413, whole genome shotgun sequence.
ACCESSION   DASPYY010000299 DASPYY010000000
VERSION     DASPYY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52413"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VEP73_04600"
     CDS             complement(<1..199)
                     /locus_tag="VEP73_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYT25747.1"
                     /translation="MDKGVRVRLEALRGEVAGAARRLLDGGLVRGTSGNVSARAGDLV
                     AVTPTGVGYRDLEPEHVTVIDL"
     gene            complement(240..995)
                     /locus_tag="VEP73_04605"
     CDS             complement(240..995)
                     /locus_tag="VEP73_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT25748.1"
                     /translation="MASEPLSAYLPLLVEGRPQAFVGLFGRNPVIEDPRAGRVSGIEA
                     VEAFAAATADWLDERDARLVPGRTTQGSARTVVESALILDDGGDGQVDLPVALVADRH
                     GDRLGAVRVYHSLWPTEGTHRVRPPLLPERADLVLPQVVRDYQAALAAGDLDAVLELF
                     GPDGYAREPSGDRYLHRGPEELARFYGGLFEAGGLPLQHCTATDDGSCTALEYNVVSW
                     GDRVLEPQAGVACYERSQNGRLVAARIYDDVEI"
     gene            complement(1001..1765)
                     /locus_tag="VEP73_04610"
     CDS             complement(1001..1765)
                     /locus_tag="VEP73_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYT25749.1"
                     /translation="MVDRLTVPGGGALPPQPGGDPRPGHRASLTELAYDALKREILTA
                     GIRPGAALIENELAARFGVSKTPIREALRLLVQDGWIVVLPRKGYLVRPLSLEDVREV
                     FALRLLLEPPVAADAATHSSEDLLERLRRAAGARQHLGAGGAVGGGEAPYRAAMRSAR
                     AFHLTLAATAGNGRLLKVLEGLLDEVERLLHLMPQLGMPQLGGHLASIAETSAHRRII
                     AAIGAGDAPAAAEQVRAHLTQSGQTLVKAFGGVSER"
BASE COUNT          270 a          759 c          790 g          251 t
ORIGIN      
        1 cgaggtcgat cacggtgacg tgctccggct cgaggtctcg gtagccgacc ccggtgggcg
       61 tcaccgcgac caggtcgccc gcccgggcgc tcacgttgcc ggaggtgccc cgcacgagcc
      121 cgccgtccag cagccgcctg gccgcgccgg cgacctcgcc acgcaacgcc tccaaccgca
      181 cccggacccc cttgtccatc cgggggctac cgctccgctg cctgagcccc cggaccccct
      241 cagatctcga cgtcgtcgta gatgcgggcg gcgaccagac ggccgttctg gctgcgctcg
      301 tagcaggcca cgccggcctg gggctcgagc acccggtcgc cccaggacac cacgttgtac
      361 tcgagcgcgg tgcagctccc gtcgtcggtc gcggtgcagt gctgcagcgg gagaccgccg
      421 gcctcgaaca gcccgccgta gaagcgcgcg agctcctcgg ggccgcggtg caggtaacgg
      481 tcgccgctcg gctcgcgggc gtacccgtcg ggcccgaaga gctcgaggac ggcgtcgagg
      541 tcgccggcgg ccagggcggc ctggtagtcc cggacgacct ggggcaggac gaggtcggcc
      601 cgctcgggca ggagcggcgg gcgcacccgg tgggtgccct cggtcggcca caggctgtgg
      661 tagacgcgga cggcgccgag gcggtcgccg tggcggtcgg cgaccagcgc gacgggcagg
      721 tcgacctggc cgtcaccgcc gtcgtcgagg atgagcgccg actccacgac ggtccgcgcg
      781 ctgccctggg tggtgcgccc ggggaccagg cgggcgtccc gctcgtcgag ccagtcggcg
      841 gtcgcggcgg cgaacgcctc caccgcctcg atccccgaga cgcgcccggc gcgggggtcc
      901 tcgatgaccg ggttgcgccc gaacaggccg acgaacgcct ggggccggcc ctcgacgagg
      961 agcgggaggt aggcgctgag cggctcgctc gccatggcgg tcatcgctcg gacacgccgc
     1021 cgaacgcctt gacgagggtc tggcccgatt gcgtgaggtg cgcgcgcacc tgctcggcgg
     1081 ccgccggggc gtcgccggcg ccgatcgccg cgatgatgcg ccgatgcgcc gaggtctccg
     1141 cgatcgaggc aaggtgaccg ccgagctgcg gcatgccgag ctggggcatg aggtggagca
     1201 ggcgctccac ctcgtcgagc agcccctcga ggaccttgag aaggcgcccg ttgccggccg
     1261 tcgccgcgag ggtcaggtgg aaggcgcgcg ccgagcgcat cgcggcccgg tagggcgcct
     1321 caccgccgcc gaccgcgcca ccggcaccga ggtgctgcct ggcgccggcc gcccgccgga
     1381 gccgctcgag caggtcctcg gagctgtggg tggccgcgtc ggccgccacc ggaggctcga
     1441 gcagcaggcg gagcgcgaac acctcgcgga cgtcctcgag cgagaggggg cggaccaggt
     1501 accccttgcg gggcaggacc acgatccagc cgtcctggac gagcaggcgg agcgcctccc
     1561 ggatcggcgt cttgctgacg ccgaagcggg cggccagctc gttctcgatc agggcggcgc
     1621 cgggccggat gccggcggtc aggatctccc gcttgagcgc gtcgtaggcc agctcggtga
     1681 gggaggcgcg gtggcccggg cgggggtcgc cacccggttg cggcggaagc gcgccgccgc
     1741 cggggaccgt cagcctgtcg accatggtca cgcatcctga gagttgaaca gcgcagctcc
     1801 tggaaccgcg aggatcgcac gcccaacgta gaggatcgca cgcccggcca cccaaggcag
     1861 atatgcccaa agtatacggg cccggacgcc ggcggcccgg gccgtgtggc ccgggccgcc
     1921 ggcggtgcga tgcaggctac gactcctcga aggcgtcaaa ctcctccggt gcgtagtcgc
     1981 tgcccgcgtc gacctcggag agcccgccct cggcgccttc gacggtgccg atcgggttgc
     2041 cgtcctcgcc gtagtagacg ccgtcgtagc
//
LOCUS       DASPYY010000300         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1738646, whole genome shotgun sequence.
ACCESSION   DASPYY010000300 DASPYY010000000
VERSION     DASPYY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738646"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..148
                     /locus_tag="VEP73_04615"
     CDS             8..148
                     /locus_tag="VEP73_04615"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYT25750.1"
                     /translation="MGLTPDPTPRFVKRYADLRSGLADAASRFAKDVAEGHYPGPEHE
                     YH"
     gene            245..826
                     /locus_tag="VEP73_04620"
     CDS             245..826
                     /locus_tag="VEP73_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYT25751.1"
                     /translation="MSEGSAAAPAERVVDGRAVPPAGTWAIDPAHSSFEFVARHLMSK
                     VRGRFATFTGTAEVAEVPEQSTVHVELETVSVDTREPTRDGHLKSADFFDVEHYPTIT
                     FQSTGLRPADEGVWKLDGDLTIRGITRPVTFDLEFLGVGTDPWGNLRSGFSAVTKVNR
                     EDWGLTWNAPLETGGFLLAKEVRLEVETELVRK"
     gene            complement(861..1667)
                     /locus_tag="VEP73_04625"
     CDS             complement(861..1667)
                     /locus_tag="VEP73_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYT25752.1"
                     /translation="MRVVTDARGRGVELPDRPERIVSLVPSLTEWLAAAGVGERLVGV
                     TDWCVEPADVTAGLPKVRGTKNPDLRAVTALAPDLVVANAEENRKVDVERLEAAGVPV
                     VVTMPTTVAGAVRELRTLATAVGDLPRAPAIDAELTAALAEAYRSSARARPVRYACAV
                     WRDPWMWVGRRTYAADLLRLAGGEPVFDDPATRYPKASPAEIAALGPRLVLLPSEPYA
                     FGRADADEVAALTGADVRLADGRALTWYGPRIPAALAAFRELLAGAAPGR"
     gene            complement(1670..2218)
                     /locus_tag="VEP73_04630"
     CDS             complement(1670..2218)
                     /locus_tag="VEP73_04630"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYT25753.1"
                     /translation="MRIYTRGGDDGTTGLLYGGRVDKSDPRTEAYGTTDEAVAALGMA
                     RVFIADGMLADLVLKLQRELFVVGAELATAVENAHKLTPGRTKVTAAMVDSLEALIDE
                     YIGKIRMPEEFIVPGESRGSSFLDYARTVIRRAERHTVAMDRAGLLAGSEAVRYLNRL
                     ADLVFVLARYEEGDFRTLRSES"
     gene            complement(2215..2925)
                     /gene="npdG"
                     /locus_tag="VEP73_04635"
     CDS             complement(2215..2925)
                     /gene="npdG"
                     /locus_tag="VEP73_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYT25754.1"
                     /translation="MADQLTLGLLGGTGPQGRGLALRFALAGHEVLLGSRSAERAREV
                     VAGLDAGRGLPIRGVANEEAAARAEVVLLTFPYEGQAVVLPALAGVIGSKVVVDVVNP
                     LGWDRQGPYLLDVPEGSAAEQARALLPDARVTAAFHHTAPRLLADPDREVDTDVLVAG
                     DDLEAKAVVIALADQIPGCRGVDAGPLRLARQLEGFTAVLVSVNRRYRIHSGVRITRL
                     AEERRQRPPAPAAARGRS"
     gene            complement(3021..>3468)
                     /locus_tag="VEP73_04640"
     CDS             complement(3021..>3468)
                     /locus_tag="VEP73_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYT25755.1"
                     /translation="LDAVPAGPSTLVGSLLEPLMRRGEVAGAPLGHPWGDAGTLGRFL
                     AVSAGLLAGCWPFALPPGRLLAPAGPGRGAVLVAEGAVLEPGAVVAGPVLLDAGSRVG
                     AGAVVTRAVVGPGATVAAGATVTGSVLGPGATVAPGSVVAAGLLPG"
BASE COUNT          459 a         1322 c         1293 g          394 t
ORIGIN      
        1 cgacctgctg ggactcaccc ccgaccccac gccgcgcttc gtcaagcgct acgccgacct
       61 gcgctcgggg ctggccgacg cggcctcgcg gttcgccaag gacgtcgcgg aggggcacta
      121 cccgggaccg gagcacgagt atcactgagt ccctcgatgg ttgcggcaac aagggagtgt
      181 gagatagttg cccctgcaac cgtcgtggcc gcgcagacgt ggccacagct gagaggagaa
      241 cagcatgagc gagggttccg ccgcggcgcc agcggagagg gttgtcgacg ggcgtgcggt
      301 gccgccagcg ggcacatggg ccatcgaccc ggcccactcg tcgttcgagt tcgtcgcccg
      361 ccacctgatg tccaaggtgc gcgggcgctt cgccaccttc accggcacgg ccgaggtggc
      421 cgaggtgccc gagcagtcca ccgtccacgt ggagctcgag acggtcagcg tcgacacacg
      481 cgagccgacc cgcgacggtc acctcaagtc cgcggacttc ttcgacgtcg agcactaccc
      541 gacgatcacg ttccagagca ccgggctgcg gcccgccgac gagggcgtct ggaagctcga
      601 cggcgacctc acgatcaggg gcatcacccg gccggtcacc ttcgacctcg agttcctcgg
      661 ggtcgggacc gacccgtggg gcaacctgcg ctccggcttc tcggccgtca ccaaggtcaa
      721 ccgcgaggac tggggcctca cctggaacgc gccgctcgag accggcgggt tcctcctcgc
      781 caaggaggtg cggctcgagg tcgagaccga gctcgtccgc aagtagcggc cgcccgaaga
      841 ttcccgggac tccggggtcc tcagcggccc ggcgccgcgc ccgcgagcag ctcgcggaag
      901 gcggcaaggg cagccgggat ccgcggtccg taccaggtca gggcgcggcc gtcggccagg
      961 cggacgtcgg ccccggtgag cgcggcgacc tcgtcggcgt cggcccggcc gaacgcgtac
     1021 ggctccgagg gcagcagcac gaggcggggg ccgagcgcgg cgatctcggc cgggctcgcc
     1081 ttcgggtagc gggtggcagg gtcgtcgaag accggctccc cgccggccag gcggagcagg
     1141 tcggccgcgt aggtgcggcg cccgacccac atccacgggt cgcgccagac cgcgcaggcg
     1201 tacctgaccg gacgggccct tgccgaggac cggtacgcct cggccagggc cgcggtcagc
     1261 tcggcgtcga tcgcgggggc gcgcggcagg tcgccgaccg cggtggccag ggtgcgcagc
     1321 tcgcgcacgg ccccggcgac cgtcgtcggc atggtcacga ccaccggcac gccggccgcc
     1381 tcgagccgct cgacgtcgac cttgcggttc tcctcggcgt tggcgaccac gaggtccggg
     1441 gcgagggccg tgaccgcgcg gaggtcgggg ttcttggtgc cccggacctt ggggagcccg
     1501 gccgtcacgt cggcgggctc cacgcaccag tcggtgaccc cgacgagccg ctcccccacc
     1561 ccggcggccg ccagccactc ggtgagcgac gggaccaggc tgacgatccg ctccgggcgg
     1621 tccgggagct cgaccccgcg cccgcgcgcg tcggtgacca cccgcacggc tatgactccg
     1681 agcgcagcgt gcggaagtcg ccctcctcgt accgggcgag gacgaacacc aggtcggcga
     1741 gccggttcag gtagcgcacg gcctcggagc cggcgagcag cccggcccgg tccatcgcca
     1801 ccgtgtggcg ctcggcccgc cggatcaccg tgcgggcgta gtcgaggaag gacgagcccc
     1861 gggactcgcc gggcacgatg aactcctcgg gcatgcggat cttgccgatg tactcgtcga
     1921 tgagcgcctc gaggctgtcg accatggcgg ccgtcacctt ggtccgcccc ggcgtgagct
     1981 tgtgggcgtt ctcgacggcg gtcgcgagct cggccccgac cacgaacagc tcccgctgga
     2041 gcttgaggac gaggtcggcg agcatgccgt cggcgatgaa caccctcgcc atgccgagcg
     2101 cggcgaccgc ctcgtcggtg gtcccgtacg cctcggtgcg cgggtcggac ttgtccaccc
     2161 gcccgccgta caagaggccg gtggtgccgt cgtcgccgcc gcgcgtgtag atcctcacga
     2221 gcgccccctc gcggccgccg gggccggcgg gcgctgccgc cgctcctccg cgaggcgggt
     2281 gatgcggacg ccggagtgga tccggtagcg ccggttcacg ctcacgagga ccgccgtgaa
     2341 cccctcgagc tgcctggcca gccggagcgg cccggcgtcg acgccccggc acccggggat
     2401 ctggtcggcg agcgcgatca ccaccgcctt ggcctccagg tcgtctccgg ccacgagcac
     2461 gtccgtgtcc acctcccggt ccgggtcggc gagcagcctc ggggcggtgt ggtggaacgc
     2521 cgccgtcacc cgcgcgtccg gcagcagcgc cctggcctgc tcggcggcgc tgccctccgg
     2581 gacgtcgagg aggtaggggc cctgcctgtc ccagccgagc gggttgacga cgtcgacgac
     2641 gaccttggac ccgatcacgc cggcgagggc gggcaggacc accgcctggc cctcgtacgg
     2701 gaacgtgagc aggaccacct cggcccgcgc ggccgcctcc tcgttggcga ccccccggat
     2761 gggcaggccg cgcccggcgt cgagcccggc gacgacctcc cgcgcccgct ccgccgagcg
     2821 cgacccgagc aggacctcgt gcccggccag ggcgaagcgc aacgcgagcc cgcggccttg
     2881 cggccccgtg ccgccgagca gaccgagggt gagctgatcg gccactatgc tgcctcccgt
     2941 cgtcgttgcc gtccggcggc gcgccggcaa gggcaggcta ccggacccgg cggggcgagt
     3001 ttgcaccgca ggcggcagcg tcagccgggc aggaggccgg ccgccacgac tgagcccggc
     3061 gcgaccgtgg cgccgggccc gaggacgctg ccggtcaccg tcgcgcccgc cgccaccgtg
     3121 gcacccgggc cgaccacggc ccgggtgacc accgccccgg cgccgacccg gctccccgcg
     3181 tcgagcagga ccgggccggc gaccacggca ccgggctcga gcacggcgcc ctcggccacc
     3241 agcaccgcgc cccggcccgg gccggcgggg gcgagcaggc ggccgggcgg cagcgcgaag
     3301 ggccagcagc cggcgagcag cccggccgac acggcgagga accggccgag ggtcccggcg
     3361 tctccccagg ggtggccgag cggcgccccc gcgacctcgc cgcggcgcat gagcggctcg
     3421 agcagcgacc cgaccagggt ggaggggccc gcggggaccg cgtccagc
//
LOCUS       DASPYY010000301         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2019992, whole genome shotgun sequence.
ACCESSION   DASPYY010000301 DASPYY010000000
VERSION     DASPYY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019992"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VEP73_04645"
     CDS             <1..906
                     /locus_tag="VEP73_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYT25756.1"
                     /translation="HPVGLLANVGGAGDAAAAVAAGAEGVGLFRTEFLYLDRADPPTE
                     AEQAAAYKAVFDAFPQGKVVVRTLDAGADKPLAFLHLGEEANPALGIRGLRATRSRPD
                     LLATQLDAIGQAAAASAAEVWVMAPMVATAEEAAWFCARARERPVAKAGVMIEIPAAA
                     LMAETVLAEADFASLGTNDLAQYTFAADRLAGALAGLQSPWQPALLKLVAAAAAGGRV
                     AGRPVGVCGEAAGDPVLALVLVGLGVTSLSMAPVSLADVRMVLGRHTLRECRRMAGLA
                     LAAPDAAEARALVRDATPTLDELGL"
     gene            1002..2003
                     /gene="glpX"
                     /locus_tag="VEP73_04650"
     CDS             1002..2003
                     /gene="glpX"
                     /locus_tag="VEP73_04650"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HYT25757.1"
                     /translation="MGVQAVLPADPDRNLALELVRATEAAAMAAARHLGRGDKELVDQ
                     AAVDAMRPVLGSIAMDGVVVIGEGEKDNAPMLYNGERVGNGSEPRVDIAVDPVDGTTL
                     TARSLPNAIAVVALAERGTMFDPGPCVYMEKLAVGRDAAAAVDFEAPIETNLARIADA
                     KGLDVTELTVCILDRPRHEEELIGRVRATGARIKFLLDGDVAGAIMAARPGTGVDVLV
                     GIGGTPEGVIAACALKCLDGALFGRLWPRNGAERRAALDAGYDLQRVLTIDDLVGGDN
                     VFFAATGVTDGELLRGVRYEHHEVLTQSLSMRSRSGAVRLIEARHQLGKSNLVTRPR"
     gene            complement(2032..>2396)
                     /locus_tag="VEP73_04655"
     CDS             complement(2032..>2396)
                     /locus_tag="VEP73_04655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYT25758.1"
                     /translation="HDTGDRLLVEVAERLTACLRADDTLARMGGDEFTVLMEDMAARD
                     EAVQVAERVLDSLRRPFLLAGQALSVSTSIGIVVSDGDETAPGDLLCQADLAMYRAKE
                     KGRSCWQLVDATSGTARR"
BASE COUNT          301 a          857 c          932 g          306 t
ORIGIN      
        1 cacccggtcg ggctgctcgc caacgtgggg ggcgccggcg acgccgccgc ggcggtcgcc
       61 gccggcgccg agggcgtggg cctgttccgc acggagttcc tctacctcga ccgcgccgac
      121 cctcccaccg aagcggagca ggcggctgcc tacaaggccg tcttcgacgc ctttccccag
      181 ggcaaggtgg tggtgcggac gctggacgcc ggggcggaca agccgctcgc gttcctgcac
      241 ctgggagagg aggcgaaccc cgcgctcggg atccgcgggc tgcgtgcgac gcggagccgc
      301 ccggacctcc tggccacgca gctcgacgcc atcgggcagg cggcagccgc gagcgccgcc
      361 gaggtctggg tgatggcgcc gatggtcgcc accgccgagg aggcggcgtg gttctgcgcc
      421 cgggcacggg agcgcccggt ggccaaggcg ggggtgatga tcgagatccc ggccgccgcc
      481 ctcatggccg agacggtcct ggccgaggcg gacttcgcga gcctcggcac caacgacctc
      541 gcccagtaca cgttcgcggc cgaccggctc gcgggcgccc tcgccgggct gcagagcccc
      601 tggcagccgg cgctgctgaa gctcgtcgcg gctgccgcgg cgggcgggcg ggtggccggg
      661 agaccggtcg gggtctgcgg ggaggcggcc ggcgacccgg tgcttgcgct cgtgctcgtg
      721 ggcctcggcg tgaccagcct ctcgatggcc ccggtgagcc tcgcggacgt gcgcatggtg
      781 ctcgggcgcc acaccctgcg ggagtgccgg cggatggccg ggctcgccct cgcggcgccg
      841 gacgccgccg aggcccgcgc gctggtccgg gacgcgacgc caacactgga cgagctcggg
      901 ctgtgatggt cgagcggggg tctggggggg catcctggag gagcccttgc tcatccgcgc
      961 ctggctcccg gcaggcgacg gcaggctacc ggaggtgacc tgtgggcgta caggcagtgc
     1021 tacccgccga cccggaccgc aacctcgcgc tcgagctggt gcgggcgacc gaggccgccg
     1081 ccatggccgc cgcccgccac ctcgggcgcg gcgacaagga gctggtcgac caggccgccg
     1141 tcgacgcgat gcgcccggtg ctcggctcga tcgcgatgga cggcgtggtg gtcatcggcg
     1201 agggggagaa ggacaacgcg ccgatgctct acaacggcga gcgggtgggc aacggctccg
     1261 agccccgggt cgacatcgcg gtcgacccgg tggacggcac cacgctgacc gccaggagcc
     1321 tgccgaacgc gatcgccgtg gtggcgctcg ccgagcgcgg cacgatgttc gacccgggcc
     1381 cctgcgtcta catggagaag ctcgcggtgg gccgggacgc cgccgccgcg gtggacttcg
     1441 aggcgccgat cgagaccaac ctggcacgca tcgctgacgc caaggggctg gacgtgaccg
     1501 agctgaccgt ctgcatcctc gaccggccgc ggcacgagga ggagctcatc gggcgggtgc
     1561 gagccaccgg cgcccgcatc aagttcctgc tcgacgggga cgtggccggc gcgatcatgg
     1621 ccgccaggcc ggggaccggc gtcgacgtgc tcgtcgggat cggcggcacc cccgagggcg
     1681 tgatcgccgc ctgtgcgctg aagtgcctgg acggagcgct gttcgggcgg ctgtggcccc
     1741 gcaacggcgc cgagcggcgc gcggcgctcg acgccgggta cgacctgcag cgggtgctca
     1801 cgatcgacga tctcgtgggg ggcgacaacg tcttcttcgc cgccaccggg gtgaccgacg
     1861 gtgagttgct gcggggcgtg cgctacgagc accacgaggt gctcacgcag tcgctgtcga
     1921 tgcgctcgag gtccggtgcg gtgcggctga tcgaggcccg tcatcagctc ggcaagtcga
     1981 acctggtgac ccggccgcgg tagcggcccg cacggcggcg cgcgtccagg ctcagcgccg
     2041 ggccgtgccg ctggtggcgt cgacgagctg ccagcacgac cgccccttct ccttcgcccg
     2101 gtacatggcg aggtcggcct ggcagagcag gtcgcctggg gcggtctcgt ccccgtcgct
     2161 gacgacgatg ccgatgctcg tggacaccga cagcgcctgg ccggcgagca ggaaggggcg
     2221 gcggaggctg tcaaggaccc gctcggcgac ctgcacggcc tcgtcgcggg ccgccatgtc
     2281 ctccatcagg acggtgaact cgtccccgcc catcctcgcc agggtgtcgt ccgctcgcag
     2341 gcaggcggtc agccgctcgg ccacctcgac gagcagccgg tccccggtgt cgtgcc
//
LOCUS       DASPYY010000302         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_194521, whole genome shotgun sequence.
ACCESSION   DASPYY010000302 DASPYY010000000
VERSION     DASPYY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194521"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VEP73_04660"
     CDS             <1..1515
                     /locus_tag="VEP73_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT25759.1"
                     /translation="GDVFATRAGPPVLQQLESAGGPDVTANDCFRPVSCYWDRINRSE
                     QLPGSVLEAMRVLTDPAETGAVTLALPQDVQTEAFDYPEELFARRVWHVPRPVPEPAV
                     LARAVEMLRGARRPLIVAGGGVIYSEATDALRALVDATGVPVAETYAGKGSMPYDHPS
                     ALGAIGVTGADGANAMAREADVVLGLGTRWGDFATASHSVFANPDVRFVNVNVASVDA
                     FKHAATPVVADARVALEALHEALAGWRADDEWRRAAAEHNRAWDAAVERVYRAGHGPL
                     PSQGEVIGALNDAAEPRDVVVCAAGSMPADLHKLWRTRDPKGFHVEYGNSCMGYEIPG
                     GLGIKLADPDREVFVMIGDASYLMAPQELVTAVAERVKLIVVLVQNHGYASIGSLSEA
                     SGSARFGTQFRYRDDDGSFEGGLLPVDLAANAASLGARVFVARSINEFRDALKAARDV
                     DRTVVVHVETDPAEYVTIGGAWWDVAVAEVSTSESVRQARATYEREKRKQRFYL"
     gene            1544..>2488
                     /gene="mmsA"
                     /locus_tag="VEP73_04665"
     CDS             1544..>2488
                     /gene="mmsA"
                     /locus_tag="VEP73_04665"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYT25760.1"
                     /translation="MRTINHWIGGREVDREPERTGAVYDPATGQQAAQVACAAGDDVD
                     AAVQAALAAFPRWRDTSVVKRARVMFAFRDLLERHRDELARIITSEHGKVVPDAAGEV
                     QRGLEVVEVASGIPHLLKGDFSENVSTEVDCYSIRQPLGVAAGITPFNFPIMVPMWMH
                     PIAIACGNCFVLKPSERDPSVSLRVAELYAEAGLPDGVFNVVHGDKAAVDGVLDHPGI
                     AAVSFVGSTPIARYVHQRASANGKRVQALGGAKNHAVVLPDADIEFAAAHITAAGYGS
                     AGQRCMAISAVVAVGDAGDALVAKLKEKAEDTKVGPGRD"
BASE COUNT          339 a          899 c          915 g          335 t
ORIGIN      
        1 ggcgacgtgt tcgccacccg ggccgggccg ccggtcctcc agcagctcga gtcggccggc
       61 gggccggacg tgaccgccaa cgactgcttc cgaccggtgt cctgctactg ggaccggatc
      121 aaccggtccg agcagctccc cggttcggtg ctggaggcga tgcgggtcct gaccgacccg
      181 gccgagaccg gcgcggtcac cctggccctg ccccaggacg tgcagaccga ggcgttcgac
      241 taccccgagg agctgttcgc ccgccgggtc tggcacgtgc cccggcccgt gccggagccg
      301 gccgtgctgg ccagggcggt cgagatgctg cggggcgcca ggcgcccgct gatcgtggcc
      361 ggcggcgggg tcatctactc cgaggccacc gacgccctgc gggcgctggt cgacgcgacc
      421 ggcgtcccgg tggccgagac ctacgccggc aaggggtcga tgccctacga ccacccgtcc
      481 gctctcgggg cgatcggggt gaccggggcc gacggggcca acgccatggc ccgcgaggcc
      541 gacgtggtgc tcgggctcgg cacccgctgg ggcgacttcg cgaccgcctc gcacagcgtg
      601 ttcgccaacc ccgacgtccg cttcgtgaac gtcaacgtcg cctcggtcga cgcgttcaag
      661 catgccgcca ccccggtggt ggccgacgcc cgggtggcgc tggaggcgct ccatgaggcc
      721 ctggccggct ggcgggctga cgacgagtgg cgccgggcgg cggccgagca caaccgggcc
      781 tgggacgccg ccgtcgagcg ggtctaccgc gccgggcacg ggccgctgcc cagccagggc
      841 gaggtgatcg gggccctcaa cgacgccgcc gagccgcgcg acgtggtcgt ctgcgcggcc
      901 ggcagcatgc ccgccgacct ccacaagctg tggcgcaccc gcgaccccaa ggggttccac
      961 gtcgagtacg ggaactcctg catgggctac gagatcccgg gcgggctcgg gatcaagctg
     1021 gccgaccccg accgcgaggt gttcgtgatg atcggcgacg cctcctacct gatggccccg
     1081 caggagctgg tcacggccgt ggccgagcgg gtcaagctga tcgtggtgct ggtccagaac
     1141 cacggctacg cgtcgatcgg ctcgctgtcg gaagcttccg gctcggcccg cttcggcacg
     1201 cagttccgct accgggacga cgacggcagt ttcgagggcg ggctcctccc ggtcgacctg
     1261 gccgccaacg cggccagcct gggcgcccgg gtgttcgtcg cccgctccat caacgagttc
     1321 cgcgacgccc tgaaggcggc cagggacgtt gaccggacgg tcgtcgtcca cgtcgagacc
     1381 gacccggccg agtacgtgac catcggcggc gcctggtggg acgttgccgt cgccgaggtc
     1441 tccacctccg agtccgtgcg gcaggcgcgg gccacctacg agcgcgagaa gcgcaagcaa
     1501 cggttctacc tctaggcgga gcaccgcgaa gggagtgcct ggcatgagga cgatcaacca
     1561 ttggatcggc ggtcgcgagg tggatcggga gcccgagcgc accggtgcgg tctacgaccc
     1621 tgccaccggg cagcaggccg cccaggtcgc gtgcgccgcc ggggacgacg tcgacgccgc
     1681 cgtacaggcg gccttggccg ccttcccgcg ctggcgggac acctcggtgg tcaagcgggc
     1741 ccgggtcatg ttcgcgttcc gcgacctgct cgagcggcac cgggacgagc tggcccggat
     1801 catcacctcc gagcacggca aggtggtgcc cgacgcggcc ggcgaggtcc agcgcggcct
     1861 ggaggtcgtc gaggtcgcat ccggcatccc gcacctgctc aagggcgact tctccgagaa
     1921 cgtctccacc gaggtggact gctactcgat ccgccagccg ctcggggtgg cggccgggat
     1981 caccccgttc aacttcccga tcatggtgcc catgtggatg cacccgatcg cgatcgcctg
     2041 cgggaactgc ttcgtgctca agccctccga gcgggacccg tcggtgtcgc tgcgggtggc
     2101 cgagctgtac gccgaggccg gcctgcccga cggggtgttc aacgtcgtcc acggcgacaa
     2161 ggcggccgtg gacggggtgc tcgaccatcc cggcatcgcc gcggtgtcgt tcgtcggctc
     2221 gacgccgatc gccaggtacg tgcatcagcg ggcgtcggcg aacggcaagc gcgtgcaggc
     2281 gctcggcggg gcgaagaacc acgccgtcgt cctccccgac gcggacatcg agttcgcggc
     2341 cgcccacatc accgccgccg gatacgggtc ggccggccag cgctgcatgg cgatctccgc
     2401 cgtggtcgcc gtgggcgacg cgggcgacgc cctggtcgcg aagctgaagg agaaggccga
     2461 ggacaccaag gtcggcccgg gccgggac
//
LOCUS       DASPYY010000303         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2301492, whole genome shotgun sequence.
ACCESSION   DASPYY010000303 DASPYY010000000
VERSION     DASPYY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301492"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VEP73_04670"
     CDS             complement(<1..295)
                     /locus_tag="VEP73_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT25761.1"
                     /translation="MKRIDCLAAVYDRLAECAVVTIMGAVAVELYSLGHRANFFYLEH
                     AMGLASSVGLGVALAQPDRRVVVIDGDGSVLMNLGGLTTLARYAPPNLTHLVFD"
     gene            complement(306..824)
                     /locus_tag="VEP73_04675"
     CDS             complement(306..824)
                     /locus_tag="VEP73_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT25762.1"
                     /translation="MPVSERSSKLVYDALLAAGVEVVSALPETWLVHLIRLAEDDPGM
                     TLVRLAKEEEGVGISAGAHLAGRKSAMLMQNHGFLQSVNGIVSLALLYKIPLLMLISD
                     RGHLGEPDPWQTEGGKATRPVLDALGIVTDELRDPAQVQLQVGRGMTLAQASLSPVAL
                     LLTRDLMWEALA"
     gene            complement(835..1044)
                     /locus_tag="VEP73_04680"
     CDS             complement(835..1044)
                     /locus_tag="VEP73_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25763.1"
                     /translation="MTEVASDLLGRATTADEDELLDLYRRCKALAARDLDPCARAGVR
                     AALVGLWNVVNDLGLEHEQLIDLGA"
     gene            complement(1041..>2088)
                     /locus_tag="VEP73_04685"
     CDS             complement(1041..>2088)
                     /locus_tag="VEP73_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HYT25764.1"
                     /translation="GTTGREPIRVLDTAKDWAWQSEMWCYGIWGFGVRPADVAYVAFG
                     YGSFIGFWGLHYGMEKIGALNVPGGAQTTEGRVKQIVDFGATVVAATPTYALRLAQEA
                     ERLGIDLAASPVSRLILSGEPAGSIPQTKALIERQWGARAGDTAGMTEIGTIMVFECA
                     HQPGGTHVIEDHFVEEVVDPATGEPVGYGERGERVVTSFGRGIIPLLRYRTADLVIRV
                     PASACTCGRGFDLYEGGILGRVDDMKLVRGTNVYPRAVEAIVREFPVVEEFQLRLTRD
                     DGMFDRIALRVELPADATDETWAEIAERLGKELADAHEGLRFDVERAATGELPRFELK
                     ARRTVDLRDKPIGA"
BASE COUNT          289 a          754 c          756 g          289 t
ORIGIN      
        1 tgtcgaagac gaggtgggtg aggttcggcg gggcgtagcg ggccagggtg gtgaggccgc
       61 cgaggttcat cagcaccgac ccgtccccgt cgatcaccac cacccgccgg tcgggctggg
      121 cgagcgcgac gcccaggccg accgaggacg cgagccccat ggcgtgctcg aggtagaaga
      181 agttggcacg gtgcccgagc gagtacagct cgaccgcgac cgcgcccatg atcgtgacga
      241 cggcgcactc cgcgagccgg tcgtagaccg ccgccaggca gtcgatgcgc ttcatcgggg
      301 cgctctcaag cgagggcctc ccacatgagg tcgcgggtga gcagcagcgc gaccggcgac
      361 agggacgcct gcgcgagggt catgccgcgg ccgacctgga gctggacctg ggcggggtcg
      421 cggagctcgt cggtcacgat gccgagcgcg tcgagcaccg ggcgggtcgc cttgccgccc
      481 tcggtctgcc aggggtcggg ctcccccagg tggccgcggt cgctgatcag catgaggagc
      541 gggatcttgt agagcagcgc gagcgacacg atgccgttga ccgactgcag gaacccgtgg
      601 ttctgcatca gcatggcgga cttcctgccg gccaggtggg cgccggcgga gatgccgacc
      661 ccctcctcct ccttggcgag ccggacgagc gtcatgccgg ggtcgtcctc ggcgagccgg
      721 atgaggtgca cgagccaggt ctccggcagc gcgctgacca cctccacccc ggccgccagc
      781 agggcgtcgt agacgagctt ggagctccgc tcggagaccg gcatcggtcc ctcctcacgc
      841 gcccaggtcg atgagctgct cgtgctcgag cccgaggtcg ttgacgacgt tccagaggcc
      901 gaccagggcg gcgcgcaccc cggcgcgggc gcacgggtcg aggtcgcggg cggcgagcgc
      961 cttgcagcgc cggtagaggt cgaggagctc gtcctcgtcg gcggtggtcg cccgcccgag
     1021 caggtcgctg gccacttcgg tcacgctccg atcggcttgt cgcgcaggtc gacggtccgc
     1081 cgcgccttca gctcgaaccg cggcagctcg ccggtcgccg cccgctccac gtcgaagcgg
     1141 aggccctcgt gggcgtcggc gagctccttg ccgagccgct cggcgatctc cgcccaggtc
     1201 tcgtcggtcg cgtcggcagg cagctcgacc cgcagggcga tccggtcgaa catgccgtcg
     1261 tcgcgggtga ggcggagctg gaactcctcg accaccggga actcgcggac gatggcctcg
     1321 accgcgcgcg ggtagacgtt ggtgccccgc acgagcttca tgtcgtcgac ccggccgagg
     1381 atgccgccct cgtacaggtc gaagccgcgc ccgcaggtgc acgccgacgc gggcaccctg
     1441 atgaccaggt cggcggtgcg gtagcgcagg agcgggatga tcccccgccc gaacgaggtg
     1501 acgacccgct ccccccgctc gccgtagccg accggctcgc cggtggccgg gtcgacgacc
     1561 tcctcgacga agtggtcctc gatgacgtgg gtcccgcccg gctggtgggc gcactcgaag
     1621 accatgatcg tcccgatctc ggtcatcccc gcggtgtcgc ccgccctggc gccccactgg
     1681 cgctcgatga gcgccttggt ctgcgggatc gagcccgccg gctcgccgga caggatgagc
     1741 cggctgaccg ggctcgcggc caggtcgatc ccgaggcgct cggcctcctg cgcgagccgc
     1801 agggcatagg tgggggtcgc cgcgaccacg gtcgcaccga agtcgacgat ctgcttgacc
     1861 cggccctcgg tcgtctgggc gccgccgggg acgttcagcg cgccgatctt ctccatgccg
     1921 tagtgcaggc cccagaagcc gatgaacgag ccgtacccga acgccacgta ggcgacgtcg
     1981 gccgggcgca cgccgaaccc ccagatcccg tagcaccaca tctcggactg ccatgcccag
     2041 tccttggcgg tgtcgagcac ccggatcggc tcccgcccgg tcgtcccc
//
LOCUS       DASPYY010000304         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2160405, whole genome shotgun sequence.
ACCESSION   DASPYY010000304 DASPYY010000000
VERSION     DASPYY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160405"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..781)
                     /locus_tag="VEP73_04690"
     CDS             complement(218..781)
                     /locus_tag="VEP73_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT25765.1"
                     /translation="MILAILRSARSAAPDPLLSEVLEAFRRQWLAFARKRYPELRDDL
                     EDAVQSALVKLVSIEKLDGLKDVARLESWARSLFVHTVLDVARESGRRRTRRAYLASP
                     DEDPEEVLREGLPADRPTPEEMAAYRERLEIVARCVEKLDVARLKFVENLPEKEIALR
                     LNLTRDGVASQLKRIRRGLRVAFGDAK"
     gene            1003..>3379
                     /locus_tag="VEP73_04695"
     CDS             1003..>3379
                     /locus_tag="VEP73_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25766.1"
                     /translation="MNAAAGPRGLLVLAALTLAIPLGASGATWPRVDVTPAISLTTTT
                     VVLPTTTLTLPVTTTSVTLPVTTLTLPLTTTSVTLPVTTLTLPLTTTSLTLPVTTLTL
                     PLTTTSVTLPTTTLTLPLSTTTTRSPTTTTASTATTTRPPTTTSTTGPPATAPLFELA
                     QTLRPARTPSSVPERMGAALAASGGTFVIGAPGDSSEAPDAGIVYVVSASSPTFGRLL
                     RVLRKPGTPAAGDAFGAAVAAIGQSVLVGAPGDNTGAPGAGAAFAFGPTDSTPTTLLP
                     PAPTAYGAFGAAVADVAGDLAVGAPGTGTVHLFRRATGEPLLTIESPPGAGRFGSAID
                     AVGANILVGAPGSGDAPGKAFLYAGGTGSLLLTLTGATAGDQFGFAVATDGSRLLIGA
                     PGAGAVFLFSFKGEPIRTFVKPTPRAGDRFGASIAVTAAGLLVGAPSDGEVAPAGGAA
                     YLFDLETGELRGVIRKEGARPEDLFGTAVAGFGAHVLLGAPGDDAGLVDGGAAYLLSG
                     GTLEAVFRKRLSAAAFGASVAAAGADVLVGAPRGEGGAGAVGRFDGASGAALATLESP
                     APGHPGFGFSVAALGNNVVAGAPFLATAGGGDVGAVYRFAGTALAGTLVDPTPLGGDQ
                     FGFSIATVGTDVVVGVPLAGARDTGVAYLLDGPSGALRVTFQKPIPVAGDFFGAAVAA
                     EGDEVLVGAPLDSETAPKAGAAYLFRRDTATLERPIFQSPAPAAGDLFGAAVALAGPR
                     VVIGAPQAAGGATDAGAVYVFDRASRNLLVTIPSPMPHRGDQFGSAVAAAGD"
BASE COUNT          432 a         1316 c         1176 g          455 t
ORIGIN      
        1 ggcgagctcg tggaggaggc tcagcacggc ccagcagtcg gagcagcgac tgacatgctc
       61 ctctgcctcc cgacgctcgc gcgccgccag cgtcggatcc ccggcccgac cctgaaccga
      121 gccgagcagg atcgccatgc actcctggtg attcacgacc acatccctat gacgaccggc
      181 cgaccccggt tgtggcggtt cgccaccggc cgggggctca tttcgcatct ccgaaggcga
      241 cgcgcagccc cctgcggatg cgcttcagct ggctcgccac gccgtcccgg gtcaggttca
      301 gccgcagcgc gatctccttc tccggcaggt tctcgacgaa cttcagccgg gcgacgtcca
      361 gcttctccac gcaccgcgcc acgatctcga gacgctcccg gtaggcggcc atctcctccg
      421 gggtggggcg atccgccggc agcccctccc ggagcacctc ctcgggatcc tcgtcggggg
      481 aggcgaggta cgctcgtcgg gtccgccgac ggccgctctc ccgggcgacg tcgagcacgg
      541 tgtggacgaa gaggctccgg gcccaggact cgaggcgggc gacgtccttc agcccgtcca
      601 gcttctctat ggagacgagc ttcacgaggg cgctctggac cgcgtcctcg aggtcgtcgc
      661 gcagctcggg atagcgcttg cgggcgaagg ccagccactg ccggcggaag gcctcgagca
      721 cctcgctgag cagggggtct ggcgccgcgg accgcgcgga gcgcaggatg gctaggatca
      781 cctctggccg cgcgtccagc acgcgcgagc atggcccgag agggagcgaa gcgtcaagac
      841 gagcgcccct cccgcgccct ccctcgggaa gcgcgtagca cggcgtctgc gcggtccgtc
      901 tctcgcaaac cactgaccgg gattgacggc ctgacggcgc cgccgtacgg tcctcatcgg
      961 gtgcgtgccg agcgagcccc caacccgcgt gaacgcgcac aaatgaatgc tgccgctggg
     1021 ccgcgcggcc tgctggtcct ggcggcgctc acgctcgcga tcccgctcgg cgcctcggga
     1081 gcgacgtggc cgcgggtaga cgtcaccccg gcgatcagcc tcaccaccac gaccgtcgtc
     1141 ctccccacga ccaccctcac gctgcccgtc acgacgacca gcgtgaccct gccggtgacc
     1201 acgctcacgt tgccgctcac gacgaccagc gtgaccctgc cggtgaccac gctcacgctg
     1261 ccgctcacga cgacgagcct gaccctgccg gtgaccacgc tcacgctgcc actcacgacg
     1321 accagcgtga ccctgccgac caccacgctc acgctgccgc tcagcacgac gacgacacgg
     1381 tcgccaacca ccaccaccgc atccaccgcc acgaccacgc gcccgccgac cacgacctcc
     1441 accaccggtc cgcccgcgac ggcgcccctc ttcgagctcg cccagacgct gcggccggcc
     1501 cgcacaccgt cgtcggtgcc cgagcggatg ggcgcggcgc tcgccgccag cggcggcacg
     1561 ttcgtcatcg gggcgcccgg tgatagcagc gaggcgcccg acgccgggat cgtctacgtg
     1621 gtcagcgcgt cgagcccgac gttcggccgc ctgctccgcg tcctcagaaa gcccgggacg
     1681 cccgccgcgg gcgacgcctt cggtgcggcg gtggcggcca tcggtcaaag cgtgctggtc
     1741 ggcgcgcccg gagacaacac gggggcgccc ggtgccgggg cggccttcgc cttcggcccc
     1801 acggattcca cccccacgac cctgctcccg ccggcaccca cagcatacgg cgcgttcggc
     1861 gccgcggtcg ccgatgtggc cggcgacctc gcggtcggcg cgccggggac cggcacggtc
     1921 cacctcttcc gtcgcgcgac gggcgagccg ctgctcacca tcgagagccc cccgggagcc
     1981 ggccggttcg gctccgcgat cgatgcggtg ggcgcgaaca ttctcgtcgg cgcgccggga
     2041 agcggcgacg cccccggcaa ggctttcctc tatgcaggcg gtaccggcag tctcctgctg
     2101 acgcttaccg gagcgacggc cggggaccag ttcggctttg cggtggcgac cgacgggagc
     2161 cgcctgctga tcggtgcccc cggggcgggc gcggtcttcc tcttcagctt caaaggtgag
     2221 ccgatccgga ccttcgtgaa accgacgccg cgcgcaggcg accgcttcgg ggcctcgatc
     2281 gcggtcaccg cagcgggcct cctggttggc gccccgtccg acggcgaagt ggcgccggcg
     2341 ggcggcgcgg cctacctgtt cgatctggag accggagagc tgcgtggcgt gatccgcaag
     2401 gaaggggcgc ggcccgagga tctcttcggc acggcagtgg ccgggttcgg cgcccacgtg
     2461 ctcctcggcg cccccggcga cgacgccggg ctggtcgacg gcggtgccgc ctatctcctc
     2521 tccggcggga cgctcgaggc ggtgttccgc aagcgcctgt cggccgcggc cttcggtgcg
     2581 tcggtcgcag ccgcgggcgc cgacgtcctg gtcggcgcgc cgcggggcga gggcggggca
     2641 ggagcggtcg gccgcttcga cggcgcgagc ggcgcggccc tggccaccct cgaaagcccc
     2701 gccccgggcc acccgggctt cgggttctcg gtcgcggcac tcggcaacaa cgtcgtcgcc
     2761 ggtgctccct tcctggcgac cgccgggggc ggcgacgtcg gtgccgtata ccgcttcgcc
     2821 gggacggccc tcgccggcac cctcgtcgat ccgacacccc tcggcggcga tcagttcggc
     2881 ttctcgatcg ccacggtggg caccgatgtc gtggtcggcg tccccctcgc tggcgcgcgg
     2941 gacacgggcg tggcctacct cctcgacggc ccgagcggcg cgttgcgcgt gaccttccag
     3001 aagccgatcc cggtggcagg agacttcttc ggcgcggccg tcgcggcgga aggtgacgag
     3061 gtgctggtcg gcgcgccgtt ggacagcgag acagccccga aggccggcgc ggcctacctc
     3121 ttccgccgcg acacggcgac gctcgagcgc cccatcttcc agagcccggc gcccgccgcc
     3181 ggcgacctgt tcggtgccgc ggtggcgctc gcgggtccca gggtcgtcat cggcgcgccc
     3241 caggccgccg ggggggcgac cgatgccggc gcggtctacg tcttcgaccg cgcgagccgc
     3301 aacctgctcg tcaccatccc gagcccgatg ccccaccggg gcgaccagtt cggcagcgcg
     3361 gtcgccgccg cgggggatt
//
LOCUS       DASPYY010000305         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1599467, whole genome shotgun sequence.
ACCESSION   DASPYY010000305 DASPYY010000000
VERSION     DASPYY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599467"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VEP73_04700"
     CDS             <1..568
                     /locus_tag="VEP73_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25767.1"
                     /translation="MGRPGLGLSEAALAALEGHDWPGNVRELAHCLRQAAALTGGAVI
                     TAEDLRLPSPEPRRDDTADDAAVLDCLRQHGFDMQATARALGWDRSTVTQRLKGLGFR
                     ALVEAGGDRTRAALALAGDAALGRAVELKLREYHEHLLRAIQGFDSPDAAVGACRRRF
                     KNLPERHFRALELLVRQHLERRPPTDTA"
     gene            807..1418
                     /locus_tag="VEP73_04705"
     CDS             807..1418
                     /locus_tag="VEP73_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYT25768.1"
                     /translation="MVNAQQEAGTKSGRVKVVVIVGIILAVVAATIATHRMRWAGVEP
                     EVDAATPAPAETPARVRVLAPAPVASEAVHADVYFDFKSIRLKAEAVRVLQQQAALMD
                     RTSTWGVLVQGYADRQGPAEYNKALAQRRAEAVKQFLVELGVPEPSVKTVTLGPDAAL
                     CDEPTRECLQLNRRVHIEIRRLAGAAAAVRPLADGDVSPVLGQ"
BASE COUNT          222 a          559 c          583 g          201 t
ORIGIN      
        1 gatggggcgt cccggcctgg ggctgtccga ggcggccctg gccgcgctcg aggggcacga
       61 ctggcccggc aacgtgcgcg agctcgcgca ctgcctgcgc caggcggccg cgctcaccgg
      121 cggggcggtc atcaccgcgg aagacctgag gctgccctcg ccggagcccc ggcgggacga
      181 caccgccgac gacgcggctg tgctcgactg cctgcggcag cacgggttcg acatgcaggc
      241 gacggcgcgc gcgctgggct gggaccggag cacggtgacg cagcggctca agggcctggg
      301 gttccgcgcg ctggtcgaag ccggcggcga ccggacgagg gccgcgctgg cgctggccgg
      361 tgacgcggcg ctcggccgcg ccgtggagtt gaagctgcgc gagtaccacg agcacttgct
      421 gcgagccatc cagggattcg actctcccga cgccgccgtc ggcgcgtgcc ggcggcgctt
      481 caagaacctc cccgagcgcc acttccgcgc tctcgagctc ctggtccgtc agcacctcga
      541 gcgccggccc ccgaccgaca cggcctgacc cgcgcgccgg gatgcgggga agggcccggc
      601 cggtgcgggg gatgcgggga ttccccgcgt cgccctcgcc gactttctct ccccgcttga
      661 gccaagccag cgacgcagct agattttttc cggccttcgc tgtcgagccg acctggcctg
      721 cgcgttgctc tccacatttg acctggggcc gatccgcagg ggcggacaat cggcgcccga
      781 tgacaccgaa cagaggagaa agcgccatgg tcaacgcgca gcaagaagcc gggacgaaga
      841 gcggaagggt gaaggtggtg gtgatcgtcg ggatcatcct ggcggtcgtc gccgcaacga
      901 tcgcgacgca ccggatgcgc tgggccggtg tggagcccga ggtcgacgcc gccacgccgg
      961 caccggccga gacgccggca cgggtccggg tcctggcccc ggcgcccgtc gccagcgagg
     1021 ccgttcacgc cgacgtctac ttcgacttca agagcatccg cctcaaggcc gaggccgtcc
     1081 gcgtcctcca gcagcaggcg gcgctcatgg accggacgag cacctggggg gtgctggtcc
     1141 agggctatgc cgaccgccag gggccggccg agtacaacaa ggcgctcgcc cagcgccggg
     1201 cggaggcggt caagcagttc ctcgtcgagc tcggggtccc ggaaccctcg gtgaagacgg
     1261 tcacgctcgg accggacgcg gcgctctgcg acgagcccac ccgggagtgc ctgcagctca
     1321 accggcgggt gcacatcgag atccggaggc tcgccggcgc cgccgcggcc gtccggccgc
     1381 tcgctgacgg cgacgtctcg cccgtgctcg gccagtgagg ccagccccat gagatcgacc
     1441 agcaagctct ggatgctcgt gatcctgctt tgcgccctga tatcgccggc ggtcgccagg
     1501 gcggagggcc cgaaggctga ggcgggggtg ggcgcacgcc gcgtgacgga agggaccctg
     1561 ctctg
//
LOCUS       DASPYY010000306         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_477245, whole genome shotgun sequence.
ACCESSION   DASPYY010000306 DASPYY010000000
VERSION     DASPYY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477245"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..757
                     /locus_tag="VEP73_04710"
     CDS             56..757
                     /locus_tag="VEP73_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HYT25769.1"
                     /translation="MIKIAGLTESEVDRRLSEVARRAAPADWTILAAPGQVEIHLRER
                     VPDGRPVAALDRIDREIAGVLESHVFARDEDTMEDVVGRLLAGRGESLSSAESLTGGE
                     IAQRITRVPGASRYFRGGVVAYTEEAKRTVLGVRADTLKAEGSVGPETAGEMARGARR
                     LFGSTWALSATGYAGPQGGGPGQPPGTVFLGLSGPRGEATRALSLPGTRETVRSRTAR
                     TALDLLRRAILETAA"
     gene            774..1367
                     /gene="thpR"
                     /locus_tag="VEP73_04715"
     CDS             774..1367
                     /gene="thpR"
                     /locus_tag="VEP73_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYT25770.1"
                     /translation="MRLFVAAELPAGLRDRLGSVQESLRQAPLPVRWVRPEGIHLTLK
                     FLGEAPETRLGEITAALRAAGEGIAAFHLEACGAVPFPVRGTPRLVWVETRGDREAAR
                     RLAAAIDAATARIGFPAEAREYRAHLTLGRVKGPGRGEWRAMLERVAGQAAGPFDVRE
                     YVLFHSRPGPEGAVYTALQRFPLAPDAAGDPCPGVDR"
     gene            1373..>1624
                     /locus_tag="VEP73_04720"
     CDS             1373..>1624
                     /locus_tag="VEP73_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HYT25771.1"
                     /translation="MTALCLGLAFVLGSLPFSQLIAKRRGVDLRSVGSGNVGATNLGR
                     ALGLGAGAAGLLLDAAKGAAAVGLAQAILGEAATPAVTAG"
BASE COUNT          190 a          568 c          646 g          220 t
ORIGIN      
        1 cgaggaatcg gtgctgccgc gcctgggccc ggtcgccgag cgcttcgcgt accgggtgat
       61 caagatcgcg ggcctgacgg aatcggaggt ggaccggcgg ctctccgagg tggcgcgccg
      121 cgccgcgccg gccgactgga cgatcctggc ggcgccgggg caggtggaga tccacctgcg
      181 cgagcgcgtg cccgatggcc ggccggtcgc cgccctcgac cggatcgacc gcgagatcgc
      241 cggcgtgctg gagtcccacg tgttcgcccg cgacgaagat acgatggaag acgtcgtcgg
      301 tcgcctgctg gccggccggg gtgagagtct gtcctctgcc gagtcgctca ccggaggtga
      361 gatcgcccag aggatcactc gcgtgcccgg cgcctcgcgc tacttccgcg gcggggtcgt
      421 ggcctacacc gaggaagcca agagaaccgt cctgggcgtg cgcgccgaca ccctgaaggc
      481 cgaggggtcg gtcggaccgg agacggcggg ggagatggcg cgcggcgcac gccgtctgtt
      541 cgggtcgacc tgggccctgt cggcgaccgg gtacgcgggg ccgcaaggtg gcggcccggg
      601 ccagcccccc gggaccgtct ttctcggcct ctccggtccg cgcggggagg cgacccgcgc
      661 gctgagcctg ccggggaccc gggagaccgt gcgctcgcgc accgcccgca cggcgctcga
      721 tcttctccgg cgtgccatcc ttgaaacggc cgcatgagag aggcgcgacc ggcgtgaggc
      781 tgttcgtcgc cgccgagctc ccggccgggc tgcgcgaccg gctcggctcg gtccaggaat
      841 cgctgagaca ggcgcccctg ccggtgcgct gggtgcgccc ggaagggatc catctgacgc
      901 tcaagttcct cggtgaggcg cccgagaccc gtctgggcga gatcaccgcg gccctgcggg
      961 cggcgggaga ggggatcgcg gcgttccacc tggaggcctg cggcgctgtt ccgtttcccg
     1021 tccgcggaac accgcgcctc gtctgggtcg agacccgcgg cgatcgggag gcggcgcggc
     1081 ggctggccgc ggcgatcgac gccgcgaccg cccgcatcgg cttcccggcg gaggcccgtg
     1141 agtaccgggc ccacctgacc ctcggacggg tcaagggtcc cggccggggg gagtggaggg
     1201 cgatgctgga acgtgtggcg ggacaggcgg ccggaccgtt cgacgtgcgg gagtacgtgc
     1261 tgttccacag ccggcccggt cccgagggcg cggtctacac ggcgctgcag cgcttcccgc
     1321 tcgcgccgga cgcggccggg gatccgtgtc cgggtgtcga ccggtgacgg tcatgacggc
     1381 gctctgcctc ggcttggcgt tcgtcctggg ctccctgccg ttctcgcagc tgatcgcgaa
     1441 gcggcgcgga gtcgatctcc ggtcggtcgg gagcggcaac gtgggagcga ccaacctggg
     1501 gcgggccctg ggcctcggag cgggcgccgc cggccttctg ctcgacgccg cgaagggcgc
     1561 cgcggcggtc ggcctggcgc aggccatcct cggagaggcg gcgactcccg cggtcacggc
     1621 gggc
//
LOCUS       DASPYY010000307         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1881795, whole genome shotgun sequence.
ACCESSION   DASPYY010000307 DASPYY010000000
VERSION     DASPYY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881795"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VEP73_04725"
     CDS             complement(<1..308)
                     /locus_tag="VEP73_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25772.1"
                     /translation="MASRERRRAERSKRKARSTERRAQIAARYEQRNRAARDALEPLP
                     EDERPAVVTVGAVISGLIGASVVIAYLAGATVNGERPGILQVVPPALLMGVMSFGMWR
                     V"
     gene            complement(308..988)
                     /gene="thiE"
                     /locus_tag="VEP73_04730"
     CDS             complement(308..988)
                     /gene="thiE"
                     /locus_tag="VEP73_04730"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYT25773.1"
                     /translation="MATNRDRRLRILEAARLYFVCDGLPGGRDPGPVLEAALRGGADI
                     IQLREKAPRSADKLVSLADSFRRAAADHGALFILNDRPDLVAVCGADGVHVGQEDMPV
                     AEARELAGPEALVGLSTHSREQVEAACAGTGDHRPDQLSVGPVWATPTKEGRAGTGLA
                     LIEYAAREATIPWFAIGGINPQNASSVVAAGAERIVVVRAIRDADDPEAAARSLRESL
                     EVGVGVRR"
     regulatory      1022..1135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1172..2431
                     /gene="thiO"
                     /locus_tag="VEP73_04735"
     CDS             1172..2431
                     /gene="thiO"
                     /locus_tag="VEP73_04735"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HYT25774.1"
                     /translation="MPDDTKRPSEQRDDVVVPLKRSRSPDGGVEAPDAVVVGGGAIGL
                     ACAWRAARRGVRVRVLERDHPGAGASHVAAGMLAPVSEASWGEEALMRLALASAGAWP
                     GFAAELAEDSQLEVGYEPCGALHVALDRDESEELHRRFELMGSLDLGVEWLRPRACRD
                     LEPGLVPTCAAGVHAPGEAAVDPGLLLPALTAAVERCGGQVLVEAEVTDILTDDGRLT
                     GVITGDGREHRAEHVVLASGAWSGASPWLPPLARPPVRPVKGQILTLRGNPDQPVCRR
                     IVASERVYLVPRPDGRLIVGATVEERGFDIQVTAGGVLELLREAYRALPDVSELELVE
                     TVAGLRPGTPDNAPLVGPGALDGLVLATGHYRNGILLTPVSAEAVAALLAQDPLPPEA
                     EVAHPGRFAGESAPGLVAAPTIEDARE"
     gene            2428..>2624
                     /gene="thiS"
                     /locus_tag="VEP73_04740"
     CDS             2428..>2624
                     /gene="thiS"
                     /locus_tag="VEP73_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HYT25775.1"
                     /translation="MTIELNGRTVELSPGATVADAVIEAGADGDSRGVAVAVDGQVIR
                     RVDWGKTMLRSDQSVEVVRAV"
BASE COUNT          350 a          937 c          990 g          347 t
ORIGIN      
        1 acccgccaca tgccgaacga catcacgccc atcagcaaag ccggcgggac cacctggagg
       61 atgccgggac gttccccatt caccgtggcg ccggccaggt aggcgatcac gaccgaagcg
      121 ccgatcagac cggagatcac ggcgccgacg gtgacgaccg ccgggcgctc atcctctggc
      181 aggggctcga gggcgtcacg ggcggcgcgg ttcctctgtt cgtagcgggc cgcgatctgg
      241 gcccgccgct cggtcgaccg cgccttgcgc ttggagcgct ccgctcgccg gcgctcgcgg
      301 ctggccatca gcgccgtacc cccacgccga cctcaagcga ttcgcgcagg ctgcgcgccg
      361 ccgcctccgg gtcatccgcg tcgcggatcg cccgcacgac gacgatccgc tcggcgccgg
      421 cggcgaccac cgaggaggcg ttctgggggt tgatccctcc gatcgcgaac caggggatcg
      481 tggcctcgcg ggcggcgtat tcgatcagtg cgagtccggt gccggcccgg ccttcctttg
      541 tcggtgtcgc ccacaccggc ccgacgctga gctgatcggg gcggtgatcg ccggtgcccg
      601 cgcacgccgc ctcgacctgc tcgcgggagt gggtcgagag cccgaccaac gcctcggggc
      661 cggccagctc gcgcgcctcg gcgacgggca tgtcctcctg gccgacgtgg acgccatcgg
      721 cgccgcacac cgcgaccagg tcgggccggt cgttgaggat gaacagcgcg ccgtgatcgg
      781 ccgcagcgcg gcggaaggag tcggcgagcg agaccagctt gtcggcggaa cgcggggcct
      841 tctcgcgcaa ctggatgatg tccgcgccgc cccgcaatgc cgcctcaagc acgggaccgg
      901 gatcgcggcc ccccgggagg ccatcgcaga cgaagtagag ccgcgccgcc tccaggatgc
      961 gcagccgccg gtcgcggttg gtggccattg gccccagtat cccgcagcga cgggagtacc
     1021 atggaggcac aacgggagcc ccgtgagctg gggctgagag ggcggcatcc gggccgccga
     1081 ccgtctgaac ctgatccggg taatgccggc gcagggatgg tggcgcggaa cgccccccgg
     1141 cagtaagtgg ggaccacaca ggaaggcgac cgtgcccgac gacaccaaga ggccaagcga
     1201 gcagcgcgac gacgtcgtcg tgccgctgaa gagatcgcgc tctccagacg ggggcgtgga
     1261 ggcgccggac gccgtcgtgg tcggtggggg ggcgatcggg ctcgcgtgtg catggcgggc
     1321 ggcgcggcgc ggcgtgcggg ttcgcgtgct cgagcgcgac cacccgggcg caggagcctc
     1381 gcacgtggcg gcgggcatgc tcgccccggt cagcgaagcc agctggggcg aggaggcgct
     1441 gatgcgcctc gcgctggcgt ccgccggagc ctggcccggt ttcgccgccg agctcgccga
     1501 ggacagccag ctggaggtcg gctacgagcc ctgcggcgcg ctccacgtcg ccctcgatcg
     1561 cgacgagtcc gaggagttgc accgccgctt cgagctgatg ggctcgctcg acctcggcgt
     1621 cgagtggctg cgcccccgcg cctgcaggga cctggagccg gggctcgtcc cgacctgcgc
     1681 cgcgggcgtt cacgcgccgg gtgaggcggc agtcgacccg ggcctgctgc tgcccgccct
     1741 gaccgccgcc gtggagcgtt gcgggggcca ggtgctcgtc gaagccgagg tcacggacat
     1801 cctgaccgac gacggtcggc ttaccggggt gatcaccggc gacgggcgcg agcaccgcgc
     1861 cgagcacgtt gtgctggcct ccggtgcctg gtcgggagct tcgccctggc tgccgcccct
     1921 tgcccgaccc ccggtgcgcc ccgtgaaggg ccagatcctc accctccggg ggaatccgga
     1981 ccagccggtc tgccggcgca tcgtcgcctc cgagcgggtc tacctggtgc cccgtcccga
     2041 cggtcgcctg atcgtcgggg cgaccgtcga ggagcgcggc ttcgacatcc aggtcaccgc
     2101 ggggggcgtg ctcgagttgc ttcgcgaggc ctaccgcgcc cttcccgacg tttcggagct
     2161 ggagctggtc gagacggttg cgggcctgcg accagggacc ccggacaacg cgccgcttgt
     2221 cggtccgggt gcgctggacg gcctggtact ggccacgggc cactaccgga acggcatcct
     2281 gctgaccccg gtctccgccg aagcggtcgc cgcgctgctt gcccaggatc cgctaccccc
     2341 cgaggccgag gttgcccacc cggggcgctt cgcgggcgag agcgcccccg gcctggtggc
     2401 cgcaccaacc atcgaggacg cgcgggaatg acgatcgagc tgaacggacg gacggtcgag
     2461 ctgtccccgg gcgccacggt cgccgatgcc gtgatcgagg cgggagccga cggcgacagt
     2521 cgcggcgtcg cggtcgcggt cgacgggcag gtgattcgcc gggtggactg ggggaagacg
     2581 atgcttcgga gcgaccagag cgtggaggtg gtccgtgcgg tcca
//
LOCUS       DASPYY010000308         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_335256, whole genome shotgun sequence.
ACCESSION   DASPYY010000308 DASPYY010000000
VERSION     DASPYY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335256"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VEP73_04745"
     CDS             <1..985
                     /locus_tag="VEP73_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HYT25776.1"
                     /translation="GHQGQAGYVNAVLRRLAAAGPRWPDPARDPVGWATTRGSHPAWI
                     VEEALERLGPDELVALVEADNARPAVTLRATPGRATRDELLAELAAAGVEAAPSGRSP
                     DCVELARGDPRELAAVRDGRAVVQDAASALVAPALGVGRGAVVVDLAAGPGGKSGQLA
                     ALGARVVAVELHPARARLVRETADRLGPAAAGRLLAVVGDGTRPPVHAGQADGALVDA
                     PCTNLGSLRRRPEARWRHQPEEVEPLAALQLRLLEAAADAVRPGGAVVYSVCTWTRAE
                     TDAVADALLARRADLEPAPLAGPAGPAPRRQLWPHRDGTDGLFLARFAKIR"
     gene            1127..2053
                     /locus_tag="VEP73_04750"
     CDS             1127..2053
                     /locus_tag="VEP73_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT25777.1"
                     /translation="MVLIADDDRDIVRFVEVNLRLEGFEVVTAHDGQEALSRAFALQP
                     NLILLDVMMPRMDGYEVCAKLRADGRSAHIPVIMLTAKSLSADKVLGLTAGADDYIIK
                     PFDPMELVARVKTTLRRASEMRALSPLTGLPGNHRIELEIQQRMEFGTPIAVAYADLD
                     HFKSYNDRYGFLRGDEVISMAAQVLRHAADEAAGPAGFVGHIGGDDFVVVIPPEAAEP
                     FAHLAMSGFDARIPSLYDPEDAKAGVIEVEDRQGQPRCFPIVSVSLGIAANTRRRFVD
                     HRELVATATELKSLAKREPGSAYAMDRRADDA"
     gene            complement(2102..>2700)
                     /locus_tag="VEP73_04755"
     CDS             complement(2102..>2700)
                     /locus_tag="VEP73_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT25778.1"
                     /translation="GGLAAPVGGFLLGEACRQAAAWRGELGAGRPLCVSVDLSVQLAQ
                     PGLVEGVGEMVAGAGLEPGSLRLEVTERNLAGDPDAIETTLRALKRLGVALAVDQFGT
                     GSSSLATLRRLPVDALKVDRSFVAGLGRERDDTVIVAAVAGLARALGIQAQADGVETP
                     GQLDELRALGCDVAQGPHLFEPRTPAALADLVRGERRR"
BASE COUNT          333 a         1057 c         1018 g          292 t
ORIGIN      
        1 cggccaccag ggacaggccg gctacgtcaa cgcggtgctg cgccgcctgg ccgccgccgg
       61 gccgcgctgg cccgacccgg cccgcgaccc ggtcggctgg gcgacgaccc gggggtcgca
      121 cccggcctgg atcgtcgagg aggcgctcga gcgcctcggc ccggacgagc tcgtcgcgct
      181 ggtggaggcc gacaacgccc gcccggcggt caccctgcgc gccaccccgg gccgggccac
      241 ccgcgacgag ctgctcgccg agctcgccgc ggccggcgtc gaggcggccc cgagcgggcg
      301 ctcccccgac tgcgtggagc tcgcccgcgg cgacccgcgc gagctcgccg cggtgcggga
      361 cggcagggcg gtcgtccagg acgccgcctc ggccctggtg gcgcccgcgc tgggggtcgg
      421 gcggggcgcg gtcgtggtcg acctggccgc cgggccgggc ggcaagtcgg ggcagctcgc
      481 cgccctcggc gcccgggtgg tcgcggtcga gctgcacccg gcccgcgccc ggctcgtgcg
      541 cgagaccgcc gaccgcctcg gaccggcggc cgcgggccgg ctgctcgcgg tcgtcggcga
      601 cggcacccgg ccgccggtcc acgccggcca ggccgacggc gcgctggtgg acgcgccctg
      661 cacgaacctc ggcagcctgc gccgccgccc ggaggcccgc tggcgccacc agcccgagga
      721 ggtcgagccc ctggccgccc tccagctccg gctgctcgag gcggccgccg acgccgtccg
      781 cccgggcggc gcggtcgtct actcggtctg cacctggacc cgggccgaga cggacgcggt
      841 cgcggacgcc ctgctcgcgc ggagagccga cctcgagccg gccccgctcg ccggcccggc
      901 cggcccggcg ccgcggcggc agctctggcc gcaccgggac ggcacggacg gcctcttcct
      961 cgcccgcttc gccaagatcc gatgacgaag gtcaccctcc cgcctcacgt cacggccccc
     1021 gcggccgata gcattcccag acgcgctccc aggccgcccg gaagcgggcg aaccggaagg
     1081 agcgacgtgg tccgacgggc gggagagcgg tagcggcgtg gacgagatgg tgctcatcgc
     1141 agacgacgac cgggacatcg tccggttcgt cgaggtgaac ctgcgcctcg agggcttcga
     1201 ggtggtcacc gcccacgacg gccaggaggc gctgtccagg gcgttcgcgc tgcagccgaa
     1261 cctgatcctg ctcgacgtga tgatgccccg gatggacggc tacgaggtgt gcgcgaagct
     1321 gcgggccgac ggccgcagcg cgcacatccc ggtgatcatg ctgacggcca agtcgctctc
     1381 ggccgacaag gtcctcggcc tgaccgcggg tgccgacgac tacatcatca agccgttcga
     1441 cccgatggag ctggtcgcca gggtcaagac caccctgcgc cgggccagcg agatgcgggc
     1501 gctgtcgccg ctcaccgggc tgcctggcaa ccaccgcatc gagctcgaga tccagcagcg
     1561 gatggagttc gggacgccca tcgcggtcgc ctacgccgac ctcgaccact tcaagtccta
     1621 caacgaccgg tacggcttcc tgcgcggcga cgaggtcatc tccatggccg cccaggtgct
     1681 gcggcacgcc gccgacgagg cggctgggcc ggccggcttc gtcggccaca tcggtgggga
     1741 cgacttcgtg gtggtcatcc cgcccgaggc cgccgagccg ttcgcccacc tggccatgag
     1801 cggcttcgac gcccggatcc cgagcctcta cgaccccgag gacgccaagg ccggcgtgat
     1861 cgaggtggag gaccgccagg gccagccgcg ctgcttcccg atcgtgtcgg tgtcgctggg
     1921 catcgcggcc aacacccgtc gccgcttcgt cgaccatcgc gagctggtcg ccaccgcgac
     1981 cgagctgaag agcctcgcca agcgcgagcc cggctccgcc tacgcgatgg accgccgcgc
     2041 cgacgacgcc tgacccggga ggcccgtccg gcgcgggccg cccgaggcgc ctccgggccg
     2101 gtcagcggcg gcgctcgccg cggacgaggt cggccagggc ggcgggcgtg cggggctcga
     2161 acaggtgggg gccctgggcc acgtcgcagc cgagcgcgcg cagctcgtcg agctggccgg
     2221 gcgtctccac gccgtcggcc tgggcctgga tcccgagggc acgtgccagg ccggcgacgg
     2281 cggcgacgat caccgtgtcg tcccgctccc ggccgaggcc ggccacgaag gagcggtcca
     2341 ccttgagggc gtccaccggc aagcgccgga gcgtcgcgag cgacgacgag ccggtgccga
     2401 actggtcgac cgcgagcgcg accccgagcc gcttgagcgc gcgcagggtg gtctcgatgg
     2461 cgtcggggtc gccggcgagg ttccgctcgg tcacctcgag ccgcagggag ccgggctcga
     2521 gtccggcccc ggccaccatc tcgcccacgc cctccacgag cccgggctgg gcgagctgga
     2581 ccgacaggtc gaccgagacg cacaggggcc ggccggcgcc gagctcgccc cgccacgccg
     2641 cggcctggcg gcaggcctcg ccgagcagga agccgccgac cggcgcggcg agcccgccgg
//
LOCUS       DASPYY010000309         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_617027, whole genome shotgun sequence.
ACCESSION   DASPYY010000309 DASPYY010000000
VERSION     DASPYY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617027"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="coaE"
                     /locus_tag="VEP73_04760"
     CDS             <1..634
                     /gene="coaE"
                     /locus_tag="VEP73_04760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291639.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYT25779.1"
                     /translation="MLLVGLTGGIASGKSTVSAMLAERGAEVVDADLIARQVVTPGTP
                     AWHKIRDHFGPDVIAPDGTVDRQALAGMVFADKGKLALLNEITHPEIFERIAERLEAS
                     RDRAVVVVLDAALLVETGLADGVDVLVVVDAARDAQLGRLAAKGVGARDAEARIAAQL
                     PAEQRRRRADHVIENTGSLEELGTQVDELWRELERRLHEGRGPAPVIRLP"
     gene            692..1429
                     /locus_tag="VEP73_04765"
     CDS             692..1429
                     /locus_tag="VEP73_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYT25780.1"
                     /translation="MNDAVPRPPAAVFFDAGDTLLAPAPSFEGRFIEVAQAEGVSFPE
                     AEVAASVAAAARRAVWPTDWTDPATQRAFWHGYYLEILDALGHEGDREALAGALFESF
                     TDPAAYKLFGDARPTLEALAERGVKLGVISNFEPWLQTVLELEGVSHLFAAVAISGVV
                     GVAKPEPGIFLAALDQAGVEAASSLHVGDHPGMDVVGARAVGMTGVLIDRYERVPDPP
                     PGGLRVESLLELLDLVGAGPPRAREAP"
     gene            1438..2295
                     /locus_tag="VEP73_04770"
     CDS             1438..2295
                     /locus_tag="VEP73_04770"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HYT25781.1"
                     /translation="MDRGFLRFLVSADNALPQGQWVAELTPELLSWLGSVDDELRDGF
                     AYPILAAWIERGEYPPDELRAMARRMVENLESGLGEAGTDSVFLRTFSALILMEIVAY
                     DNASPFLDADEVLDLFERGLSYLRREQDLRGWVPGPGWAHAIAHVADLLMMLARSRHL
                     GAPELERLLDAVADKLLAPAEAVYLHHEDERLAYTTLNVLRRGLVGRDFLTAWAGRFE
                     RPPGHDSWRPVYADPGLAAARMNVTSFLRSLYFQLLLTAEPPPGRAGTLDALVASLRH
                     MDIGFYELN"
     gene            complement(2361..>3129)
                     /locus_tag="VEP73_04775"
     CDS             complement(2361..>3129)
                     /locus_tag="VEP73_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYT25782.1"
                     /translation="AAVLAEGRTIIGNAACEPHVQDLCRFLGALGARIDGIGSNRLTI
                     TGVERLSGGEYTIGPDHIEVASFIGLAAATGGELVVEGVIEDDLRPVAVGFTRLGVRW
                     ELEGTTLRVPPDQKLTIQDDLGAAIPKIDDGPWPAFPADLTSIAVAVATQARGTVLIF
                     EKMFENRLFFVDKLVGMGARIIICDPHRAVVNGPTRLYGERLASPDIRAGMALLLAAL
                     AAEGESVIGNIGEIDRGYERIDERLRALGASIERATG"
BASE COUNT          384 a         1205 c         1117 g          423 t
ORIGIN      
        1 catgctgctc gtcggactca ccggaggcat cgcgtcgggc aagagcaccg tctcggccat
       61 gctcgccgag cgcggcgccg aggtcgtcga cgccgacctc atcgcccgcc aggtggtcac
      121 gccgggcacc ccggcctggc acaagatccg cgaccacttc gggcctgacg tcatcgcccc
      181 cgacggcacc gtcgaccgcc aggccctcgc cgggatggtg ttcgccgaca agggcaagct
      241 cgcgctgctc aacgagatca cccacccgga gatcttcgag cggatcgccg agcggctcga
      301 ggcgtcgcgc gaccgcgcgg tcgtggtggt cctcgacgcg gccctgctcg tcgagaccgg
      361 cctggccgac ggcgtcgacg tgctcgtcgt cgtcgacgcg gcccgcgacg cccagctcgg
      421 gcgcctggcc gccaagggcg tcggggcgcg cgacgccgag gcgcggatcg ccgcgcagct
      481 ccccgccgag cagcgccgcc gccgggccga ccacgtcatc gagaacaccg gctcgctcga
      541 ggagctcggc acgcaggtgg acgagctctg gcgggagctc gagcgccgcc tccacgaggg
      601 ccggggcccg gcgcccgtca tccgcctgcc ctagctggcg ccccggcggc tggtacggga
      661 atcgcgccga cggctggtgt agcctccgcg cgtgaacgac gccgtcccgc gcccgcccgc
      721 cgccgtgttc ttcgacgccg gcgacacgct cctcgcgccc gcccccagct tcgagggccg
      781 gttcatcgag gtcgcccagg ccgagggggt gtcgttcccc gaggcggagg tggccgcctc
      841 ggtcgccgcc gcggcccgcc gggcggtgtg gccgaccgac tggaccgacc ctgccaccca
      901 gcgggccttc tggcacggct actacctgga gatcctcgac gcgctggggc acgagggcga
      961 ccgggaggcc ctcgcggggg cgctgttcga gtcgttcacc gacccggccg cgtacaagct
     1021 gttcggcgac gcccgcccca cgctcgaggc cctggcggag cgcggcgtca agctcggggt
     1081 catctccaac ttcgagccct ggctccagac ggtgctggag ctcgaggggg tcagccacct
     1141 gttcgcggcg gtcgcgatct cgggcgtcgt cggcgtcgcc aagcccgagc cgggcatctt
     1201 cctcgccgcc ctcgaccagg ccggcgtcga ggctgccagc tcgctgcacg tcggggacca
     1261 cccgggcatg gacgtggtcg gcgcccgggc cgtcggcatg accggggtgc tgatcgaccg
     1321 ctacgagcgg gtccccgacc cgccgccggg agggctccgc gtcgagtcgc tcctcgagct
     1381 gctcgacctc gtcggggccg ggccgccgcg ggcccgcgag gccccgtgat ggaggcggtg
     1441 gaccgggggt tcctccggtt cctggtcagc gccgacaacg ccctgcccca ggggcagtgg
     1501 gtcgccgagc tcacgcccga gctgctctcc tggctcgggt cggtcgacga cgagctgcgg
     1561 gacgggttcg cctacccgat cctcgccgcc tggatcgagc ggggcgagta cccgcccgac
     1621 gagctgcggg cgatggcacg ccgcatggtc gaaaacctgg aatccggcct cggcgaggcc
     1681 ggcaccgact cggtgttcct gcgcaccttc tcggccctga tcctcatgga gatcgtcgcc
     1741 tacgacaacg ccagcccgtt cctcgacgcc gacgaggtgc tcgacctctt cgagcgcggc
     1801 ctgtcctacc tgcgccggga gcaggacctg cgcggctggg tgccggggcc tggctgggcc
     1861 cacgccatcg cccacgtcgc cgacctgctg atgatgctcg cccgcagccg ccacctcggc
     1921 gctcccgagc tcgagcggct gctcgacgcc gtcgccgaca agctgctcgc gccggccgag
     1981 gccgtgtacc tccaccacga ggacgagcgg ctcgcctaca cgacgctcaa cgtgctgcgc
     2041 cgcgggctgg tcggccggga cttcctgacc gcctgggcgg gccgcttcga gcggccccct
     2101 ggccacgact cctggcggcc ggtctacgcc gacccgggcc tggccgccgc ccggatgaac
     2161 gtgacgagct tcctgcgcag cctgtacttc cagctcctcc tgaccgccga gccgcccccc
     2221 ggccgggccg ggacgctcga cgcgctggtg gccagcctgc ggcacatgga catcggcttc
     2281 tacgagctga actagcccgg cggcccacga gccgagccgg cccggcgtcc cggcccggcc
     2341 gccgggggct cgtccagccg tcagccggtg gcgcgctcga tgctcgcgcc gagggcgcgc
     2401 aggcgctcgt cgatccgctc gtagccccgg tcgatctcgc cgatgttgcc gatcaccgac
     2461 tcgccctcgg cggccagggc ggcaagcagc agggccatgc ccgcccggat gtcgggcgag
     2521 gcgagccgct ccccgtagag ccgggtcggc ccgttcacca cggcgcggtg cgggtcgcag
     2581 atgatgatcc gcgcgcccat ccccacgagc ttgtcgacga agaacagccg gttctcgaac
     2641 atcttctcga agatgagcac ggtgcctctg gcctgcgtgg ccaccgcgac cgcgatcgag
     2701 gtgaggtcgg cagggaaggc gggccacggc ccgtcgtcga tcttggggat cgccgcgccg
     2761 aggtcgtcct ggatggtgag cttctggtcg ggcggcacgc gcagcgtcgt gccctcgagc
     2821 tcccagcgca ccccgaggcg ggtgaagccg accgcgaccg ggcgcaggtc gtcctcgatc
     2881 acgccctcga cgaccagctc gccgccggtg gcggcggcca ggccgatgaa gctggccacc
     2941 tcgatgtggt cgggcccgat cgtgtactcg ccgccggaga gccgctcgac gccggtgatc
     3001 gtcagccggt tcgagccgat gccgtcgatc ctggctccga gcgcgcccag gaaccggcag
     3061 aggtcctgga cgtggggctc gcaggccgcg ttgccgatga tcgtccggcc ctccgcgagc
     3121 accgccgcc
//
LOCUS       DASPYY010000310         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2723860, whole genome shotgun sequence.
ACCESSION   DASPYY010000310 DASPYY010000000
VERSION     DASPYY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723860"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEP73_04780"
     CDS             <1..424
                     /locus_tag="VEP73_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25783.1"
                     /translation="TGVDQAVAVLDFLATVEPSARRSLVPMADQLARLSGEGLLLGVL
                     TIPTPEEAAALARCRLGFGGAMALLLRPDTWLGLAARDVAAADARAAGAAAMLERAGW
                     RTAQMIRTDRLETPWQRLIAPLGRAAPTRPRPSRSPAS"
     gene            349..>1921
                     /locus_tag="VEP73_04785"
     CDS             349..>1921
                     /locus_tag="VEP73_04785"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYT25784.1"
                     /translation="MATPDRAAGSSSTDPAQALALAGLLTGASLSLSRIYAGNGWWLP
                     TWLTMLSALGLAALLRRAGAGQAVSLLVMLAGFFVVAGVLLFPDTLLVVVPTLATGRA
                     MADAARVALQGVVDQAAPVTVTREFLLLTCAGAWAVATSADGLTFRARQPLLALVPAL
                     GLFVFPAVVRNSSPAWYTLWFLLGAAAVLLTEGRARLATWGQWVSSPRSRPASGWRLP
                     LTPAASTGRWLAVGAGVLALTVPWLLPGYGREPILDYHSGVAATGPVSINPFVSLRTR
                     LRSSQEQTLFKVRAVEGSYWRLLTLDEFDGTNWTASERPSDAAPFTPGVSELDQDPKA
                     PAKDLTQRFEILGLRGSGGDAFLPAAATPVQVRADQPILRTTVSRGLLTRRRLRSGFA
                     YTVTSRILLPDARDLDGAQDYRDTPASGYARIDNLSQRVRDQAHALTDGQPSAYRKAL
                     ALQDWLRGPLFTYDLNVPRLSSGGDQLTRFLFQERRGYCEQFASAMAAMARAVGLPAR
                     VAIGFTPGRQVGDTWE"
BASE COUNT          208 a          759 c          696 g          258 t
ORIGIN      
        1 gacaggggtg gaccaggccg tcgcggtcct cgacttcctc gccacggtgg agccgagcgc
       61 caggcgctcc ctggtgccga tggccgacca gctcgcccgc ctgtccggcg aggggctcct
      121 gctcggggtc ctcaccatcc cgacgccgga ggaggcggcc gccctggccc gctgccgcct
      181 cggcttcggc ggggcgatgg ccctgctgct gcggccggac acctggctcg gcctcgccgc
      241 ccgcgacgtc gccgcggccg acgcccgggc cgcgggcgcg gcggccatgc tggagcgcgc
      301 cggctggcgc acggcccaga tgatccgcac cgaccgactg gaaacaccat ggcaacgcct
      361 gatcgcgccg ctgggtcgag cagcaccgac ccggcccagg ccctcgcgct cgccggcctc
      421 ctgaccggcg cgtcgctgtc gctcagccgc atctacgccg gcaacggctg gtggctgccg
      481 acgtggctca cgatgctgtc ggccctgggg ctggccgcgc tgctccgccg ggccggcgcc
      541 gggcaggccg tctcgctcct cgtcatgctc gccgggttct tcgtggtggc cggcgtcctg
      601 ctgttccccg acaccctgct cgtggtcgtg ccgacgctcg cgaccgggcg cgccatggcc
      661 gacgcggcgc gggtggccct gcagggcgtc gtcgaccagg cggcgcccgt gaccgtcacc
      721 cgcgagttcc tgctgctcac ctgcgccggc gcgtgggccg tcgcgacctc ggcggacggg
      781 ctgacgttcc gggccaggca gccgctgctc gccctcgtcc ccgcgctcgg gctgttcgtg
      841 ttcccggcgg tcgtgcgcaa ctcgagcccg gcctggtaca ccctctggtt cctgctcggc
      901 gccgccgccg tgctgctcac cgaggggcgc gcccggctcg cgacctgggg ccagtgggtg
      961 tcgagcccgc gcagccgccc ggcgagcggc tggcggctgc cgctcacccc ggccgcgtcc
     1021 accgggcgct ggctcgccgt cggcgccggc gtgctcgcgc tcaccgtgcc gtggctgctg
     1081 cccggctacg gccgtgagcc gatcctcgac taccactcgg gcgtcgcggc caccggcccg
     1141 gtgtccatca acccgttcgt ctcgctgcgc acgcggctgc ggtcgagcca ggagcagacc
     1201 ctgttcaagg tgcgggccgt ggagggcagc tactggcgcc tgctcaccct cgacgagttc
     1261 gacgggacga actggacggc gtcggagcgg ccgagcgacg cggccccgtt caccccgggc
     1321 gtgtccgagc tggaccagga cccgaaggcg ccggcaaagg acctcaccca gcggttcgag
     1381 atcctcggcc tgcggggcag cggcggggac gcgttcctgc ccgccgcggc caccccggtg
     1441 caggtgcgcg cggaccagcc catcctccgg accacggtca gccgcgggct gctcaccagg
     1501 cgccggctcc gcagcgggtt cgcctacacc gtgacgtcgc ggatcctgct gcccgacgcc
     1561 agggacctcg acggggccca ggactaccgc gacacgccgg cttcggggta cgcgcggatc
     1621 gacaacctga gccagcgggt ccgcgaccag gcccacgcgc tcacggacgg gcagccgagc
     1681 gcgtaccgca aggcactcgc cctgcaggac tggctgcggg gaccgctgtt cacgtacgac
     1741 ctgaacgtcc cccgcctcag ctcgggaggc gaccagctca ccaggttcct gttccaggag
     1801 cgcaggggct actgcgagca gttcgcctcg gccatggccg cgatggcgcg cgccgtcggg
     1861 ctgcccgccc gggtcgcgat cggcttcacc ccgggccggc aggtcggcga cacctgggag
     1921 g
//
LOCUS       DASPYY010000311         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1460988, whole genome shotgun sequence.
ACCESSION   DASPYY010000311 DASPYY010000000
VERSION     DASPYY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460988"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..945
                     /locus_tag="VEP73_04790"
     CDS             4..945
                     /locus_tag="VEP73_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25785.1"
                     /translation="MRPTAGGGTGPTVELRHVSKWYGDVVAVADLSFELGPGVTGLLG
                     PNGAGKTTTLKAVTGLLHPSQGEVLLFGRPIAKDPMLYRAIGVVPDGERLYPRLTVRS
                     YVRLHADLQGLAAPGAAADRAIAEVGMEALADRRLKGLSKGERQRTKVAGAIVHQPSL
                     LVLDEPMTGMDPGQRARFIELVRLVARAGVTVLVSSHILGEVERLASQILVLVGGRLA
                     ASGDFHAIRDLMQDQPRTVVIRAGNARALAPGLIAMPSTVAVEVEGDAIRARTRSVEA
                     FATELPRLARAQGITLREVRTTDESLEQVFRYLVGRR"
     gene            942..1670
                     /locus_tag="VEP73_04795"
     CDS             942..1670
                     /locus_tag="VEP73_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25786.1"
                     /translation="MRGAAVVLRFALGQLLAGRRLLVTASLAAVPLLVPALFAVGSGR
                     DPASFTLDLFRALVLPVLLPVVALTFSTTALGAEVRDGTMVNIVLKPIPRPGILAAEY
                     LAAVAATLVVLLPVEVVTHLVAARGPGSTTLLAGMLLATTVGTLTYCALGVLLSLLVG
                     RALLVGLAYALLWEGALVSVAPSASSLSVRGYTEGVLASVAARAGLGFSTRLGPLSAT
                     LLALVVTLLALALAVRRLGRMDFR"
     gene            complement(1566..>1955)
                     /locus_tag="VEP73_04800"
     CDS             complement(1566..>1955)
                     /locus_tag="VEP73_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25787.1"
                     /translation="FVYLGVLSGTGAVCQARAPRELRARIASLFMLAVGGGHALGLVV
                     QGWLGDRFGLPLVTAVTGVALLAVVVVTWAPRPAWLREMDAPAAVGVGGPWSAEVHAP
                     EPADGQREREEGDDEREQRRAQRPQPG"
BASE COUNT          218 a          764 c          706 g          267 t
ORIGIN      
        1 ggcatacgcc ccacggccgg aggcggcacc ggtcccacgg tcgagctgcg ccacgtctcc
       61 aagtggtacg gggacgtggt ggccgtggcc gacctgtcct tcgagctcgg cccgggcgtg
      121 accggcctgc tcggccccaa cggggccggc aagacgacga cgctgaaggc ggtcaccggc
      181 ctgctccacc cctcccaggg cgaggtgctc ctctttggcc ggccgatcgc gaaggacccg
      241 atgctgtacc gggcgatcgg ggtcgtgcct gacggcgagc gcctctaccc ccgcctcacc
      301 gtgcgctcct acgtgcgcct gcacgccgac ctgcaggggc tggcggcgcc gggggcggcc
      361 gccgaccggg ccatcgccga ggtcggcatg gaagccctgg ccgaccgccg gctgaagggc
      421 ctgtcaaagg gcgagcgcca gcgcaccaag gtggccggcg cgatcgtgca ccagccctcg
      481 ctgctggtcc tggacgagcc catgaccggc atggaccccg gccagcgtgc ccgcttcatc
      541 gagctcgtcc ggctcgtcgc cagggccggc gtcaccgtgc tggtgtcgag ccacatcctc
      601 ggcgaggtgg agcggctcgc ctcccagatc ctcgtcctgg tcgggggccg gctggccgcc
      661 tcgggcgact tccacgccat ccgcgacctc atgcaggacc agccgaggac ggtggtcatc
      721 agggccggca acgcccgggc gctcgcgcct ggcctgatcg ccatgccgag caccgtggcg
      781 gtcgaggtgg agggcgacgc catccgggcg cgcacccgct cggtcgaggc gttcgcgacc
      841 gagctgccca ggctggccag ggcgcagggg atcacgctgc gcgaggtccg caccaccgac
      901 gagtcgctcg agcaggtctt ccgctacctg gtggggcgca gatgaggggg gcggcggtcg
      961 tgctccgctt cgccctgggc cagctcctgg ccgggcggcg cctgctggtc accgcgtcgc
     1021 tcgccgcggt cccgctcctg gtgcccgccc tgttcgcggt gggctcgggc cgggacccgg
     1081 cgagcttcac cctcgacctg ttccgcgccc tggtgctgcc ggtgctcctg cccgtggtcg
     1141 cgctcacctt ctccaccacg gccctcgggg ccgaggtgcg cgacggcacg atggtcaaca
     1201 tcgtcctcaa gccgatcccg aggccgggca tcctcgccgc cgagtacctg gccgccgtgg
     1261 ccgccaccct ggtcgtcctg ctccccgtcg aggtcgtcac gcacctggtc gcggcccgcg
     1321 gtcccggctc gaccaccctg ctggccggga tgctgctcgc caccacggtg gggacgctca
     1381 cctactgcgc cctcggggtc ctgctgtcgc tcctggtcgg gcgggcactg ctcgtcgggc
     1441 tcgcgtacgc gctgctgtgg gagggggccc tggtcagcgt ggcgccgtcg gcgtcctccc
     1501 tgtcggtgcg cggctacacc gagggcgtgc tcgcgtcggt cgcggcccgc gcggggctgg
     1561 gcttctcaac ccggctgggg ccgctgagcg cgacgctgct cgcgctcgtc gtcaccctcc
     1621 tcgcgctcgc gctggccgtc cgccggctcg ggcgcatgga cttccgctga ccagggcccg
     1681 cccaccccga cggcggccgg cgcgtccatc tcccgcagcc aggccggccg gggcgcccac
     1741 gtgaccacga cgaccgcgag gagggcgacg ccggtgacgg cggtcacgag cggcagcccg
     1801 aaccggtccc cgagccaccc ctgcacgacg aggccgagcg catggccgcc cccgaccgcc
     1861 agcatgaaca ggctcgcgat gcgggcccgc agctcgcgcg gggccctggc ctggcagacc
     1921 gcgcccgtcc ccgacagcac gccgaggtag acgaa
//
LOCUS       DASPYY010000312         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_756750, whole genome shotgun sequence.
ACCESSION   DASPYY010000312 DASPYY010000000
VERSION     DASPYY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756750"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2133)
                     /locus_tag="VEP73_04805"
     CDS             complement(<1..>2133)
                     /locus_tag="VEP73_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYT25788.1"
                     /translation="GTESTCVLVLEATCRLIYSPPVRRLVVLGFPDVYQAGDAVPAVL
                     EHRPSGLEGIDDQLIGYMRNIGLHPDEVDLLPPGRGWLLVELATEDGAEATGRVRDLV
                     DGLGRQGVHPSVATFDDPAQAALIWTVRESGLGATARVPGQRPMWEGWEDSAVPPERV
                     GDYLRDLRGLLDRYGYKGSFYGHVGQGCMHTRNDFDLQSADGVARFRAYVEEAADLAV
                     AYGGSLSGEHGDGQARAELLPKMFGPELVEAFREFKTIWDPEGRMNPGKVVDPYRLDE
                     NLRLGAGYRPLELATTFRFPADRGGFADAALRCVGVGKCRRERGGTMCPSYMVTREEQ
                     HSTRGRARLLFEMLEGEVITDRWRSKEVHDALDLCLSCKGCKGDCPVSVDMATYKAEF
                     LSHYYRRRLRPRAAYSIGLIWWAARAAGRAPRLANLLTQTPLVRQVVKRLGGIAPARP
                     IPPFAGEPFTTWFRRRGPGRPGGRPVLLWPDTFNNWFHPETAKAAVEVLEAAGFRVEL
                     PARALCCGRPLYDFGMLSLAKRQLRQVLAAVGPRIDQGVPLVGLEPSCLATFRDELVN
                     LFPDDERAERLQRQSHTLAELLARHAGGWTPPPAAARAIVQGHCHQKAVIGMRPDTAL
                     MASMGLSADVLDAGCCGMAGSFGFEREHYDVAMRVGERALLPAVRAADPATLVVADVF
                     SCRTQIEQATGRPTLHLAEVLRDALRATG"
BASE COUNT          281 a          775 c          815 g          262 t
ORIGIN      
        1 ccggtggcgc ggagcgcgtc gcggaggacc tcggcgaggt gcagggtcgg ccggccggtg
       61 gcctgctcga tctgggtgcg gcagctgaac acgtccgcga ccaccagcgt ggcggggtcg
      121 gccgcccgca cggcgggcag cagggcccgc tcaccgaccc gcatggccac gtcgtagtgc
      181 tcgcgctcga agccgaacga gccggccatg ccgcagcagc cggcgtcgag gacgtccgcc
      241 gagaggccca tggacgccat cagcgcggtg tcggggcgca tgccgatgac cgccttctgg
      301 tggcagtggc cctggacgat ggccctggcc gcggccggcg gcggcgtcca gccgccggcg
      361 tggcgggcga gcagctccgc gagggtgtgc gactgccgct ggaggcgctc ggcccgctcg
      421 tcgtcgggga acaggttgac gagctcgtcc cggaaggtgg cgaggcagct cggctcgagc
      481 ccgacgaggg gcacgccctg gtcgatccgc ggcccgaccg cggccaggac ctggcggagc
      541 tgccgcttgg cgagcgacag catcccgaag tcgtacagcg gccgcccgca gcacagtgcc
      601 ctcgcgggga gctcgacccg gaagccggcc gcctccagca cctcgaccgc ggccttcgcg
      661 gtctcgggat ggaaccagtt gttgaaggtg tccggccaga gcagcaccgg gcggccgcct
      721 ggcctccccg ggccacgccg gcggaaccag gtcgtgaacg gctcgccggc gaacggcggg
      781 atcgggcggg ccggtgcgat cccgccgagc cgcttgacca cctggcggac cagcggggtc
      841 tgggtgagga ggttggcgag ccgcggcgcc cggccggcgg ccctggccgc ccaccagatc
      901 agcccgatcg agtacgccgc gcgcgggcgc agccgccgcc tgtagtagtg ggacaggaac
      961 tccgccttgt aggtggccat gtcgaccgac accgggcagt cgcccttgca gcccttgcag
     1021 gagaggcaga ggtcgagggc gtcgtggacc tccttcgagc gccagcggtc ggtgatcacc
     1081 tcaccctcga gcatctcgaa cagcaggcgc gcgcggcccc gggtcgagtg ctgctcctcg
     1141 cgggtcacca tgtagctcgg gcacatggtc ccgccgcgct cgcggcggca cttgccgacc
     1201 cccacgcagc gcagcgcggc gtcggcgaag ccgccccggt cggccgggaa ccggaacgtg
     1261 gtggcgagct cgaggggccg gtacccggcg ccgaggcgca ggttctcgtc cagccggtag
     1321 gggtcgacga ccttgcccgg gttcatcctg ccctccgggt cccagatcgt cttgaactcg
     1381 cggaacgcct cgacgagctc ggggccgaac atcttcggca gcagctccgc ccgcgcctgg
     1441 ccgtcgccgt gctcgcccga cagggacccg ccgtaggcga cggcgaggtc ggcggcctcc
     1501 tcgacgtagg cgcggaaccg cgccacgccg tcggcgctct gcaggtcgaa gtcgttgcgg
     1561 gtgtgcatgc agccctgccc gacgtggccg tagaacgagc ccttgtagcc gtagcggtcg
     1621 agcagcccgc gcaggtcccg caggtagtcg ccgacccgct cgggcgggac ggccgagtcc
     1681 tcccacccct cccacatcgg ccgctggccg ggcacgcgcg cggtggcgcc gaggccggac
     1741 tcccgcaccg tccagatgag cgccgcctgg gccgggtcgt cgaaggtcgc gacggacggg
     1801 tggacgccct gccggcccag gccgtcgacg aggtcgcgga cccgcccggt ggcctcggcg
     1861 ccgtcctcgg tggccagctc gaccagcagc cagccgcggc cgggaggcag caggtcgacc
     1921 tcgtcggggt gcaggccgat gttgcgcatg tagccgatga gctggtcgtc gatgccctcg
     1981 agcccgctcg gccggtgctc gaggaccgcg gggaccgcgt cgccggcctg gtagacgtcg
     2041 ggaaagccga gcacgaccag ccggcgcacc ggcgggctgt agatcagccg gcaggtcgcc
     2101 tccaggacga ggacgcaggt gctctcggtg ccg
//
LOCUS       DASPYY010000313         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1038309, whole genome shotgun sequence.
ACCESSION   DASPYY010000313 DASPYY010000000
VERSION     DASPYY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038309"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="acnA"
                     /locus_tag="VEP73_04810"
     CDS             <1..729
                     /gene="acnA"
                     /locus_tag="VEP73_04810"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551480.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HYT25789.1"
                     /translation="EPKPLLDILGARVLAVVGDSVTTDHISPAGDIALNSPAGRYLVS
                     KGIEKKDFNSYGSRRGNDRVMVRGTFANIRLKNLMVPGVEGGVTVHVPSGERVDIYDA
                     AERYRAEGTPLVVIAGKEYGSGSSRDWAAKGTLLLGVRAVLAESYERIHRSNLVGMGV
                     LPLQFKAGQNAESLGLTGLEKLTIAGIAGELRARQDVTVAVERGDGTKSSFTATARLD
                     TPVEINYYRNGGILTTVLRKMLKG"
     gene            823..1002
                     /locus_tag="VEP73_04815"
                     /pseudo
     CDS             823..1002
                     /locus_tag="VEP73_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1027..1116)
                     /locus_tag="VEP73_04820"
                     /pseudo
     CDS             complement(1027..1116)
                     /locus_tag="VEP73_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1228..>4053)
                     /locus_tag="VEP73_04825"
     CDS             complement(1228..>4053)
                     /locus_tag="VEP73_04825"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HYT25790.1"
                     /translation="EVGSRTYEIYRLDALQSKFDVARLPFSLKVLLENLLRNEDGSVV
                     RAEDVEAFARWDPAAGNDREIAFSPARVLMQDFTGVPAVVDLAAMRDAMRDLGGDQSR
                     INPLAPAELVIDHSVIADVAGTRDAFVRNAELEFQRNAERYAFLRWGQQAFADFRVVP
                     PDTGIVHQVNLEYLARVVFTNDRGQAYPDTLVGTDSHTPMVNGLGVLGWGVGGIEAEA
                     AMLGQPISMLMPKVVGFRLRGELPEGSTATDLVLTVTELLRRHGVVGKFVEFYGPGVG
                     NVPLANRATIGNMSPEYGSTCAIFPIDDETLRYLEFTGRPPELIGLVEAYAKEQGLWH
                     DPDRDPVYSETVELDLATVVPSLAGPRRPQDRVALTDAKRSFRVALKEMLGQGSRGKA
                     GTPQAQGPAGDGWESAANGNLDGTDEAVAETFPASDPPAQMAAAGARAEDNAPPDEQR
                     DRGDASDRPHASVTVEGADFTLDHGDVVIAAITSCTNTSNPSVMLAAGLLARNAVEKG
                     LTAKPWVKTSLAPGSKVVMDYYERAGLVPYLEQLGFHLVGYGCTTCIGNSGPLRPEIS
                     DAVDRGSLAVASVLSGNRNFEGRINPDCRMNYLASPPLVVAYALAGSLDADLLEEPLG
                     TGGDGEPVYLRDVWPSSAAVSELVGSSIQSDMFRKSYAEVFKGDERWSSLEVPTGDTF
                     QWDPASTYVRRPPYFDGMPSQPEPVTDVHGARVLAVLGDSVTTDHISPAGVIKRDSPA
                     GRHLMEQGVEPKDFNSYGSRRGNHEVMIRGTFANIRLRNRLVPGTEGGVTLHLPEGTR
                     TTIYEAAMRYQEEGVPLVILAGKEYGSGSSRDWAAKGTRLLGVRAVLAESYERIHRSN
                     LVGMGVLPLQFAEGESAESLGLTGREVYDVEGLEGSGTDGFPREVTVTVDGDRRFTAV
                     VRIDTPNEVQYFRHGGILRYVLRQLRG"
BASE COUNT          567 a         1402 c         1503 g          581 t
ORIGIN      
        1 gagccgaagc ccctcctcga catcctgggc gcgcgggtgc tggccgtggt cggcgactcc
       61 gtcaccaccg accacatctc ccccgcgggg gacatcgcct tgaacagccc ggccggacgc
      121 tacctcgtgt ccaaggggat cgagaagaag gacttcaact cctacggatc gcgtcgcggg
      181 aacgaccgcg tgatggtgcg cggcaccttc gcgaacatcc gcctcaagaa cctgatggtc
      241 ccgggagtgg agggcggggt cacggtgcac gtcccttccg gcgagcgcgt ggacatttac
      301 gacgcggccg agcgctaccg ggcggaaggg acccccctgg tggtgatcgc ggggaaggag
      361 tacgggagcg gctcctcgcg ggactgggcg gccaagggga ccctgctcct gggggtgcgg
      421 gccgtgctcg cggagagcta cgagcgcatc caccgcagca acctggtggg gatgggggtg
      481 ctgcccctgc agttcaaggc cggccagaac gcggagtccc tcggcctcac cggcctggag
      541 aagctcacca tcgccggtat cgcgggcgag ctgagggccc ggcaggacgt cacggtcgcg
      601 gtggagcgcg gggacggcac gaagtcgtcc ttcaccgcca ccgcccgcct cgacaccccg
      661 gtcgagatca actactaccg caacgggggc atcctcacga ccgtgctccg gaagatgctc
      721 aagggctgac gcggccgcgc catggcacgc ctcaccttcc tcggcgcggc caagacggtc
      781 accggctcgc agtacctgat cgagaccggg tccgcccggg tcatggtcga ttgcggcatg
      841 ttccagggcg acaagccgct gcgccagcgc aactgggggg agccggagta cgaccccgcg
      901 cgggtggaag ccctggtcct cacccacacc cacctcgacc acatcggccg ggtgccccgg
      961 ctggtcaagc agggcttccg ggggcccctc tggtgcacgc ccgccacccg ggagctggcc
     1021 gagatcctgc tgctcgaccc actgccggaa ggccccgacg tagttgccga gatgcgtgtt
     1081 gccggtcggc tggacgccgg aaaggatccg gggcacgagg gtcctccact gcagggtgcc
     1141 ggtaggggcc tggccgcgag ggcgggcggg gccgcctggc acggccgcgg cggcccctcc
     1201 cggcatccta caaggtctcc cgggcgctca gccgcggagc tggcgcagga cgtaccggag
     1261 tattccgccg tggcggaagt actgcacctc gttcggggtg tcgatgcgga ccacggcggt
     1321 gaaccgcctg tcgccgtcga ccgtcaccgt gacctcgcgc gggaagccgt cggttcccga
     1381 gccctcgagg ccctcgacgt cgtagacctc gcggccggtc aggccgaggg actccgccga
     1441 ctcgccctcg gcgaactgca gcggcagcac gcccatgccc actaggttgg agcggtggat
     1501 gcgctcgtag ctctcggcca gcacggcccg caccccgagc agccgggtcc ccttggccgc
     1561 ccagtcccgc gaggacccgg agccgtactc cttgccggcc aggatcacca gcggcacgcc
     1621 ctcctcctgg tagcgcatgg cggcctcgta gatggtggtc cgggtccctt cgggcaggtg
     1681 cagggtgacg ccgccctcgg tgccggggac gagccggttg cgcagccgga tgttcgcgaa
     1741 cgtgccgcgg atcatcacct cgtggttgcc ccggcgcgag ccgtaggagt tgaagtcctt
     1801 cggctcgacc ccctgctcca tcaggtggcg cccggccggg ctgtcacgct tgatgacgcc
     1861 ggccggggag atgtggtcgg tcgtcacgct gtcgcccagc accgcgagga cccgggcgcc
     1921 gtggacgtcg gtgaccggct ccggctggga cggcatgccg tcgaagtagg gcggacggcg
     1981 cacgtaggtc gacgccgggt cccactggaa ggtgtcgccg gtcggcacct cgagcgagct
     2041 ccagcgctcg tcgcccttga acacctcggc gtagctcttg cggaacatgt ccgactggat
     2101 cgacgacccg accagctcgc tgaccgcggc gctggacggc cagacgtcgc ggaggtagac
     2161 cggctcaccg tccccgccgg tgccgagggg ctcctcgagc aggtcggcgt ccagcgagcc
     2221 ggccagcgcg taggcgacca ccagcggcgg cgaggccagg tagttcatgc ggcagtcggg
     2281 gttgatgcgt ccctcgaagt tgcggttccc ggagaggacc gaggcgaccg cgaggctgcc
     2341 gcggtcgacc gcgtccgaga tctccggacg cagcgggccc gagttgccga tgcaggtcgt
     2401 gcacccgtag ccgaccaggt ggaagccgag ctgctcgagg tagggcacca ggcccgcccg
     2461 ctcgtagtag tccatgacga ccttggagcc gggcgcgagc gaggtcttga cccacggctt
     2521 ggccgtgagg cccttctcca ccgcgttgcg ggcgagcagg ccggcggcga gcatgaccga
     2581 cgggttcgag gtgttggtgc agctcgtgat cgccgcgatg acgacgtcgc cgtggtcgag
     2641 ggtgaagtcg gcgccctcga ccgtgaccga ggcgtggggc cggtcgctgg cgtcgcccct
     2701 gtcccgctgc tcgtcagggg gcgcgttgtc ctcggcccgg gcgccggcgg ccgccatctg
     2761 ggcggggggg tcactggccg ggaaggtctc ggccacggcc tcgtcggtgc cgtccaggtt
     2821 gccgttggcg gcgctctccc agccgtctcc ggccggcccc tgcgcctgag gcgtgccggc
     2881 cttgcctcgc gacccctggc ccagcatctc cttgagcgcc acccggaacg agcgcttggc
     2941 gtcggtgagg gcgacgcggt cctgggggcg gcgcggcccg gcgaggctcg ggacgaccgt
     3001 ggcgaggtcg agctcgaccg tctccgagta gaccgggtcg cggtcggggt cgtgccacag
     3061 gccctgctcc ttggcgtacg cctcgacgag cccgatcagc tcgggcgggc ggccggtgaa
     3121 ctcgaggtag cgcagggtct cgtcgtcgat cgggaagatc gcgcaggtgg agccgtactc
     3181 gggcgacatg ttgccgatcg tcgcgcggtt ggcgagcggc acgttgccga cgccgggacc
     3241 gtagaactcg acgaacttgc cgaccacgcc gtgccggcgg agcagctcgg tgacggtgag
     3301 gacgaggtcg gtggccgtcg acccctctgg cagctcgccg cgcaggcgga acccgaccac
     3361 cttgggcatg agcatcgaga tcggctggcc gagcatggcc gcctcggcct cgatcccgcc
     3421 gacgccccag ccgagcacgc cgagcccgtt caccatgggg gtgtgggagt cggtgccgac
     3481 gagggtgtcc gggtaggcct ggccgcggtc gttggtgaac accaccctcg ccaggtactc
     3541 gaggttcacc tggtggacga tgccggtgtc gggcgggacg acccggaagt cggcgaacgc
     3601 ctgctgcccc cagcgcagga acgcgtaccg ctcggcgttg cgctggaact cgagctcggc
     3661 gttgcggacg aaggcgtccc gggtgccggc cacgtcggcg atcaccgagt ggtcgatgac
     3721 cagctcggca ggggcgagtg ggttgatcct cgactggtcg ccgccgaggt cccgcatggc
     3781 gtcgcgcatg gcggcgaggt cgaccacggc gggcacgccg gtgaagtcct gcatgagcac
     3841 ccgggccggc gagaaggcga tctcccggtc gttcccggcg gccgggtccc agcgcgcgaa
     3901 cgcctcgacg tcctcggcgc gcaccacgct gccgtcctcg ttgcggagca ggttctcgag
     3961 caggaccttg agggagaacg gcaggcgagc gacgtcgaac ttcgactgca gcgcgtcgag
     4021 gcggtagatc tcgtacgtgc ggctgccgac ctc
//
LOCUS       DASPYY010000314         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1740691, whole genome shotgun sequence.
ACCESSION   DASPYY010000314 DASPYY010000000
VERSION     DASPYY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740691"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VEP73_04830"
     CDS             complement(<1..399)
                     /locus_tag="VEP73_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT25791.1"
                     /translation="MTRSARRLTMAQALVEFLANQYSERDGVEQRLIAGCFGIFGHGN
                     VAGVGQALQQYADRLRFHQARNEQGMVHVASGYARQRNRLATFACTSSIGPGATNMVT
                     GAATATANRLPVLLLPGDVVLIPYGYHGPSM"
     gene            complement(396..1262)
                     /gene="iolB"
                     /locus_tag="VEP73_04835"
     CDS             complement(396..1262)
                     /gene="iolB"
                     /locus_tag="VEP73_04835"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HYT25792.1"
                     /translation="MRQGTQREGAFSLVLTPEQAGWAFSGLRVLELPPGGAHAFDTGG
                     DELLVLPLAGGCTVACGGERFEVEGRDDVFSRVTDFVYAPRDAHVEVASAGGGRFALP
                     SARARRRLPARYGAAEDVPVELRGAGQASRQVNNFCAPDAFEADKLIAVEVLTPDGNW
                     SSWPPHKHDQERPPEEAQLEEVYYFEIAGQDGTPGLGYQRVYSSGPDRQIDVCAEVGA
                     GDVVLIPYGYHGPSMAAPGYDLYYLNVMAGPGAERAWRFCDDPAHAWVRASWTGRATD
                     PRLPMTGPGGRR"
     gene            complement(1280..2170)
                     /locus_tag="VEP73_04840"
     CDS             complement(1280..2170)
                     /locus_tag="VEP73_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HYT25793.1"
                     /translation="MREEQYRAILEARTRHPEAIAEAAARRARPTSLFNEHGRLMMIA
                     ADHPARGALRAGPRVMAMADRVELLDRLCLALSRPGVNGLLGTPDVIEDLLLLGVLDG
                     KVVVGSMNRGGLAGTVFEIDDRFTGYDAASLAAAGCEGGKMLLRIDPGDPATVATVEA
                     CARAVSGLASHRLMAMIEPFISHRMDGRVRIDTSPEATIRAMTVAAGLGATSAYTWLK
                     VPVVDDMERVMAATTLPAVLLGGEVSVDQDAAFERWRKALKLPTVCGLVIGRSLLYPP
                     DDDVAAAVDGAVGLLQEGSR"
     gene            complement(2163..3146)
                     /gene="iolC"
                     /locus_tag="VEP73_04845"
     CDS             complement(2163..3146)
                     /gene="iolC"
                     /locus_tag="VEP73_04845"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HYT25794.1"
                     /translation="MQVGLVGAAGTTGPLDVLTMGGIGVDLYPLQVGVSLRQVESFGK
                     FLGGSATNVAVAAARYGRRSAVITRTGDDPFGAYLHDALRKFGVDDRFVTPVPGLPTP
                     VTFCEIFPPDHFPIYFYRWPKAPDLEIAADELDLDAVRAARVFWVTGTGLSAEPSRTA
                     TLAALEARARSGVTILDLDYRPMFWPSREAARTWIQRALGLVTVTVGNLDECETAVGV
                     SEPRAAAQALRDHGVDVVVVKQGPKGVLARDADTEVEVPPVPVEVVNGLGAGDAFGGA
                     LCHGLLAGWDLERLMRFCNAAGAIVASRLACADAMPDAAEVEAMLEEGVDA"
BASE COUNT          448 a         1159 c         1249 g          440 t
ORIGIN      
        1 catggagggc ccgtggtagc cgtaggggat gagcaccacg tcgccgggca ggagcagcac
       61 cggcagccgg ttggcggtgg cggtggccgc cccggtgacc atgttggtcg ccccagggcc
      121 gatcgaggag gtgcaggcga aggtggccag gcggttgcgc tggcgggcgt agccggacgc
      181 cacgtggacc atgccctgct cgttgcgggc ctggtggaag cggagccggt cggcgtactg
      241 ctgcagggcc tggccgacgc cggccacgtt gccgtggccg aagatgccga agcagccggc
      301 gatcaggcgc tgctcgaccc cgtcgcgctc gctgtactgg ttggccagga actcgacgag
      361 ggcctgggcc atcgtcagcc gccgagccga cctcgtcatc ggcgtcctcc cggtccggtc
      421 atgggcaggc gggggtcggt ggcccgcccg gtccagctcg cgcggaccca ggcgtgggcc
      481 gggtcgtcgc agaagcgcca ggcccgctcg gcgccagggc cggccatcac gttgaggtag
      541 tacaggtcgt agcccggcgc tgccatggag ggcccgtggt agccgtaggg gatgagcacc
      601 acgtcgccgg cgcccacctc ggcgcagacg tcgatctgcc ggtcgggtcc ggagctgtag
      661 acccgctggt agccgagacc gggggtgccg tcctggccgg cgatctcgaa gtagtagacc
      721 tcctcgagct gggcctcctc gggcggccgc tcctggtcgt gcttgtgggg cggccaggac
      781 gaccagttgc cgtcgggggt gagcacctcg accgcgatga gcttgtcggc ctcgaacgcg
      841 tcgggcgcgc agaagttgtt gacctggcgg ctggcctggc ccgccccgcg cagctcgacc
      901 gggacgtcct cggccgcgcc gtaccgcgcg ggcaggcggc gcctggcccg ggcggagggg
      961 agcgcgaacc ggccgccgcc cgcggacgcg acctcgacgt gggcgtcgcg gggggcgtag
     1021 acgaagtcgg tgacgcggga gaagacgtcg tcgcggccct cgacctcgaa ccgctcgccg
     1081 ccgcacgcga ccgtgcagcc gccggccagg ggcaggacga gcagctcgtc gccgcccgtg
     1141 tcgaaggcgt gggcgccacc gggggggagc tcgaggacgc gcaggccgct gaacgcccag
     1201 ccggcctgct ccggtgtgag caccagcgag aaggcgccct cgcgctgggt gccctgccgc
     1261 aggtgcagct tgccgtcagt cacctgctcc cctcctgcag caggccgacg gcgccgtcga
     1321 cggcggccgc cacgtcgtcg tcgggggggt acagcagcga ccggccgatc accaggccac
     1381 agacggtggg gagcttgagc gccttgcgcc agcgctcgaa cgccgcgtcc tggtcgacgc
     1441 tgacctcgcc gccgagcagc accgccggca gggtggtcgc ggccatgacg cgctccatgt
     1501 cgtccaccac cggcaccttg agccaggtgt aggccgaggt ggcgccgagc ccggccgcga
     1561 ccgtcatcgc ccggatggtc gcctccgggc tggtgtcgat gcgcacccgc ccgtccatcc
     1621 ggtgggagat gaacggctcg atcatggcca tcagccggtg ggaggcgagc ccgctgaccg
     1681 cgcgagcgca ggcctcgacg gtggccaccg tggccgggtc gcccgggtcg attcgcagca
     1741 gcatcttgcc gccctcgcag cccgccgcgg ccagggaggc ggcgtcgtac ccggtgaagc
     1801 ggtcgtcgat ctcgaagacg gtcccggcca ggccgcccct gttcatcgac ccgaccacga
     1861 ccttgccgtc gagcacgccg agcagcagca ggtcctcgat gacgtccggg gtgccgagga
     1921 ggccgttgac gccagggcgc gacagggcga gacagagccg gtcgagcagc tcgacccggt
     1981 cggccatcgc catcacccgg gggccggccc gcagggcgcc acgggccgga tggtcggccg
     2041 cgatcatcat gaggcggccg tgctcgttga acagcgaggt cgggcgggcc cggcgggccg
     2101 cggcctcggc gatcgcctcg gggtgccggg tgcgggcctc gagaatggcc cggtactgct
     2161 cctcacgcat cgacgccctc ctcgagcatg gcctccacct cggcggcatc gggcatggcg
     2221 tcggcgcacg ccaggcggga ggcgacgatg gcgccggcgg cgttgcagaa ccgcatcagc
     2281 cgctcgaggt cccagccggc gagcagccca tgacagaggg cgccgccgaa cgcgtccccg
     2341 gcgccgaggc cgttcacgac ctcgaccggc acggggggga cctcgacctc ggtgtcggcg
     2401 tccctggcca gcacgccctt cgggccctgc ttgacgacca cgacgtcgac gccgtggtcg
     2461 cgcagggcct gggcggcggc cctgggctcg ctcaccccga cggcggtctc gcactcgtcg
     2521 aggttgccga ccgtgaccgt gacgaggccg agcgcccgct ggatccaggt cctcgccgct
     2581 tcgcgggagg gccagaacat ggggcggtag tccaggtcca ggatggtcac gcccgacctg
     2641 gcgcgggcct cgagcgccgc gagggtcgcg gtgcggctcg gctcggcgga caggccggtg
     2701 ccggtgaccc agaagacccg ggcggcccgg accgcgtcga ggtcgagctc gtcggccgcg
     2761 atctcgaggt cgggcgcctt gggccagcgg tagaagtaga tcgggaagtg gtcgggcggg
     2821 aagatctcgc agaacgtcac cggcgtgggc agcccgggca ccggggtgac gaagcggtcg
     2881 tcgacgccga acttccgcag cgcgtcgtgg aggtaggcgc cgaacgggtc gtccccggtg
     2941 cgggtgatga ccgcgctgcg cctcccgtac cgagcggccg ccaccgcgac gttggtcgcg
     3001 ctgccgccga ggaacttgcc gaagctctcg acctgtcgca gcgacacccc gacctgcagc
     3061 gggtagaggt cgaccccgat gccgcccatc gtcaggacgt cgagcggccc ggtcgtgccg
     3121 gctgcgccaa caagtcccac ctgcatggcg cgcgcctccc accatgctcg ccacggaccc
     3181 tcgactgcca gtgctcggag cgttactgtt gtgttatagg aatgtcatag gtactgcttg
     3241 agcgagttgc tgtcaacccc tttgttctaa catgctaacc gtatgacata ggtgcg
//
LOCUS       DASPYY010000315         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2022313, whole genome shotgun sequence.
ACCESSION   DASPYY010000315 DASPYY010000000
VERSION     DASPYY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022313"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1495)
                     /locus_tag="VEP73_04850"
     CDS             complement(185..1495)
                     /locus_tag="VEP73_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HYT25795.1"
                     /translation="MSSPTPAPMPPPRADAPDLRAVRGQPGTWLDRWLAACADLADLK
                     ARNPMLDAVIDEVDGRRIRIGGRWLTDFASCNYLGLDLDREVIEAIPAYLDRWGTHPS
                     WSRMLGSPVLYEQIETELTSLLGAEDALLLPTLTHLHAAVIPVLASAGTIFLDARAHK
                     TIYDGCVVARAHGATIRRFAHDDPAALEALLRQRTPGPRLVCMDGVNSMTGNPPRLAE
                     LARLARVHDALLYVDDAHGFGVVGERAPDEPCPYGRRGNGVVRHLDAGYDNVILTGGF
                     SKAYSSLLAFIACPAPLKDLLKIAAPPYLYSGPSPIASLAGALVGLRVNARRGDDLRA
                     ALYARTRRVLDHLDALGAATLNRSGFPIVEVPLADPDELGAAGAFLFGRGIYATLAFY
                     PGVPRQEVGFRLQLTAANTDEEVTGLLAVLDELAGRFRLQPAAS"
     gene            complement(1642..>2364)
                     /locus_tag="VEP73_04855"
     CDS             complement(1642..>2364)
                     /locus_tag="VEP73_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25796.1"
                     /translation="AVARLEEGDRLKEEFLAVVSHELRSPITSLLGIASTLRRRPALA
                     VSPDGAELLDRMVHQGNRLRDMVEQLLRASAVAANRSPVLRAGPVDLADVVGEVVADR
                     RAAEPDRPLRVHLPARCRPVRGDAEALRVVVANLVDNACKYSPPGSPVTISVEQPAGW
                     TSIAVADRGAGIPPEDRARVFTAFTQLDASATRRAGGVGLGLFLVRRLVEAMGGWARV
                     DGGADGGARFVVELPGSEPPAA"
BASE COUNT          298 a          830 c          963 g          273 t
ORIGIN      
        1 ccgggcagtg ccacaccgcg ctgcgcctgt tcggcgaggc ggtcgaggag gtcgccgccg
       61 ccgagggacc cgtcccgacg gccgccgagc tcgagccgcg gcaggagggc gacgtggtcg
      121 gcggctgacc ccgcgctggc atggcgcgtc ccggccggcc cgtcggcgcg gcgccgccgg
      181 gcggtcagct ggcggcgggc tggaggcgga accgcccggc gagctcgtcg aggacggcga
      241 gcaggccggt cacctcctcg tcggtgttgg ccgcggtgag ctgcaggcgg aacccgacct
      301 cctggcgcgg cacgccgggg tagaacgcca gggtcgcgta gatcccccgg ccgaacagga
      361 acgcgccggc cgcgccgagc tcgtcggggt cggcgagcgg cacctcgacg atcgggaacc
      421 cggagcggtt cagggtcgcg gccccgagcg cgtcgaggtg gtcgaggacc cggcgggtcc
      481 tggcgtacag cgcggcgcgc aggtcgtcgc cgcgcctggc gttgacccgc aggccgacga
      541 gcgcaccggc cagggaggcg atcggcgagg ggccggagta caggtagggc ggcgcggcga
      601 tcttgagcag atccttcagg ggggcggggc aggcgatgaa ggcgagcagc gaggagtatg
      661 ccttggagaa cccgccggtc aggatcacgt tgtcgtagcc ggcgtccagg tggcggacca
      721 cgccgttgcc gcgccgcccg tacgggcacg gctcgtccgg ggcgcgctcg ccgacgacgc
      781 cgaacccgtg cgcgtcgtcg acgtagagca gagcgtcgtg gacgcgggcg aggcgggcga
      841 gctcggccag ccgcgggggg ttgccggtca tcgagttgac cccgtccatg cagaccaggc
      901 gcgggccggg ggtgcgctgg cgcagcagcg cctcgagcgc ggcggggtcg tcgtgggcga
      961 accggcggat ggtcgccccg tgggcgcggg cgaccacgca cccgtcgtag atggtcttgt
     1021 gcgcgcgggc gtcgaggaag atcgtgcccg cgctcgccag caccgggatc accgcggcgt
     1081 ggaggtgggt cagggtgggc aggagcaggg cgtcctcggc gccgagcagg ctggtcagct
     1141 ccgtctcgat ctgctcgtag aggacggggc tgccgagcat gcgcgaccag ctcgggtggg
     1201 tgccccagcg gtcgaggtag gcggggatgg cctcgatgac ctcgcggtcc aggtccagcc
     1261 cgaggtagtt gcacgacgcg aagtcggtga gccagcgccc gccgatgcgg atgcgccgcc
     1321 cgtcgacctc gtcgatcacc gcgtcgagca tggggttgcg cgccttcagg tcggcgaggt
     1381 cggcgcaggc ggcgagccac cggtcgagcc aggtgcccgg ctggccgcgc accgcccgca
     1441 gatcgggcgc gtcggcacgc ggtggcggca tgggcgctgg ggtcggcgac gacaccttgg
     1501 acctcccggc ggcggggcgc ggtcgtcgtt gaccgagcga actgcatcat ctccgccata
     1561 ccgtaccagt gccgcaatga ctccggccgc ttcgagccga atgccaggca cgcccggcac
     1621 gccgggcggc tcagggccgg gtcatgcggc cggaggctcg ctcccgggca gctcgacgac
     1681 gaaccgggcg ccgccgtccg cgccgccgtc cacccgcgcc cacccgccca tggcctcgac
     1741 gaggcgacgg accaggaaca ggccgagccc gaccccgccc gcccgcctgg tcgccgaggc
     1801 gtcgagctgg gtgaaggcgg tgaagacgcg ggcgcggtcc tcggggggga tcccagctcc
     1861 ccggtcggcg accgcgatgc tggtccagcc ggccggctgc tccacgctga tcgtcaccgg
     1921 tgagcccggc ggcgagtact tgcaggcgtt gtcgacgagg ttggccacca ccacccgcag
     1981 cgcctcggcg tcgccgcgga ccggccggca gcgcgcgggc aggtggacgc gcagggggcg
     2041 gtcgggctcg gccgcgcgcc ggtcggcgac gacctcgccc acgacgtcgg cgaggtcgac
     2101 cgggccggcg cgcagcaccg ggctgcggtt ggcggcgacg gcgctggccc gcaggagctg
     2161 ctccaccatg tcgcgcaggc ggttgccctg gtgcaccatg cggtcgagca gctcggcgcc
     2221 gtcgggcgag acggccagcg ccggccggcg ccgcagcgtg ctcgcgatcc cgagcaggct
     2281 cgtgatcggg gagcgcagct cgtggctcac gacggcgagg aactcctcct tgaggcggtc
     2341 gccctcctcg aggcgggcga ccgc
//
LOCUS       DASPYY010000316         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1882636, whole genome shotgun sequence.
ACCESSION   DASPYY010000316 DASPYY010000000
VERSION     DASPYY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882636"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..899
                     /locus_tag="VEP73_04860"
     CDS             66..899
                     /locus_tag="VEP73_04860"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYT25797.1"
                     /translation="MSIDALTSAEISLPTRPDAARVRDRIAVLAAHVDLVGLTDNHAG
                     QPRMSPLAAVALAREQGVATVVHLSCRDRNRLALRSQVVGAAALGSEGLLCLYGDPVP
                     GVPRVRDYTTTRLLADAKAWAAPYDLAVGCVTDPFAVDTEGELALLGRKVAAGADFIQ
                     TQMVFDPARLAAFLGQAREGGKLEERRVYASVAVLRSRRMADYVNQALPGFALPEAAY
                     RQISAGGGVDLAVETAVALSELGGVDALHVMPLGDEEAAGRVAAAFRAARGAPVERGT
                     R"
     gene            1093..1317
                     /locus_tag="VEP73_04865"
     CDS             1093..1317
                     /locus_tag="VEP73_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25798.1"
                     /translation="MTTPPRWEAGARVVLAGASGDLARLQGTRATVLHVAGDVALVRF
                     DSAKLLGGRLRRELEVAAAWLVDEPPVAAV"
     gene            1416..2912
                     /locus_tag="VEP73_04870"
     CDS             1416..2912
                     /locus_tag="VEP73_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25799.1"
                     /translation="MPANEGGPPDAAAVAGVRRTPGQAFSRATGAAAVLAVAVGAVGT
                     VAVRHWGRIPFGVPSRPWYLSWPYYLFWDPRLDLGWALAALPVAALAAVVVVALAAPR
                     TRPAAAPVRGAPAEPPVRGAPAEPPVRGAPGPALSRRVRLGASAAAVFVLALAVSALG
                     GGPAAWRAPLAYAGEYPDGVRHVGAIPTFLARFSALQPSLPDFPAQHPPGATVLYALV
                     DRVWPGLTGAALATTAAACLGALVVAGLARDELGEAGDRLAVACWALAPIVVLYAATA
                     ADAMWAPVLAGSALAAHRGLERQSLAWTVAGGALLWVGSMLTFGAVLLLPFLLVRALA
                     RLGAGRAWVVRWAVVTAAVALGLAALCWAATGYEPVSAVRAVDRFWRVAPGSQRVWWI
                     WLFGDLIAFGGMLGFPLLAAFAVRAWTVVRERAWGSFEAATLASLLAGAAWGHTKGEV
                     ERMWQFLVPFAVVVAARQLLRWRVRLPVVAALLVGQALAVQVLFFTRW"
     gene            complement(2964..>3807)
                     /locus_tag="VEP73_04875"
     CDS             complement(2964..>3807)
                     /locus_tag="VEP73_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYT25800.1"
                     /translation="GWLVALLGPRFAAADRRRRLQLGLDEGVRARGLEAIGEVLAGGR
                     PRTRAELVRELAGKGVAIDAEGQAPAHLVAYAAMRGLVCRGPDLPGDEPTYVLLKDWV
                     GARPELDLEAALAELARRYVGAYGPAGAADLAAWSGIPLGQARRGLELVTGELEEVEV
                     AGERAWVAGGVEPDVPGVEDPCVRLLGQFDAYLLGYRSRELALPSRFARRIQAGGGLI
                     QPAVVVDGRVVGTWRQRRGGRRLTVTVEPFERLDRAILPGLEAEAADVGRFLGTETAL
                     AVTG"
BASE COUNT          344 a         1519 c         1448 g          496 t
ORIGIN      
        1 gcggtggtcg aggccgccca gcggcgctga cccgggccgc ggccgccgga tgttagcctt
       61 ctgccatgtc catcgacgcc ctcacctcag ccgagatctc gctgccgacc cggcccgacg
      121 cggcccgggt ccgcgaccgc atcgccgtcc tcgccgcgca cgtcgacctc gtcggcctca
      181 ccgacaacca cgccgggcag ccccggatgt cgcccctggc cgccgtcgcg ctcgcccgcg
      241 agcagggcgt ggccacggtc gtgcacctgt cctgccggga ccgcaaccgc ctcgccctgc
      301 ggtcgcaggt ggtcggcgcg gcagccctcg gctccgaggg cctgctgtgc ctctacggcg
      361 acccggtccc cggggtgcca cgggtccggg actacacgac cacgaggctc ctggccgacg
      421 ccaaggcgtg ggcggcaccg tacgacctgg ccgtcggctg cgtgaccgac ccgttcgcgg
      481 tcgacaccga gggcgagctc gccctgctcg gccgcaaggt ggcggccgga gccgacttca
      541 tccagaccca gatggtgttc gaccccgccc ggctggccgc gttcctcggg caggcaaggg
      601 agggtggcaa gctcgaggag cgccgcgtct acgccagcgt cgccgtgctg cgcagccgcc
      661 ggatggccga ctacgtgaac caggcgctgc ccggcttcgc gctgcccgag gcggcgtacc
      721 ggcagatctc ggccggcggc ggggtggacc tcgccgtcga gaccgccgtc gccctgtccg
      781 agctcggcgg ggtcgacgcc ctgcacgtca tgccgctcgg cgacgaggag gcggccgggc
      841 gggtggcggc cgccttccgg gccgcccgcg gggccccggt cgagcgcggc acccgctgac
      901 cggccctggc acgtgggccc gcccccaccg tgcccgttgc gctcgcttgt gttgacgcca
      961 tactggacaa ggcggcatgc tcgccgtagc cgttcgcgtg acccgccaac gaaggccgtc
     1021 gggagggctg gcgcccgtcc tgacggcctt tcgcgtcttg ggaggcgggt gggaggccgg
     1081 aagggagctc gcatgacgac accgccgcgg tgggaggctg gcgcccgcgt ggtgctcgcc
     1141 ggtgcgtccg gcgacctggc gcggctgcag ggcacgcgcg cgaccgtgct ccacgtggcc
     1201 ggcgacgtcg cgctggtccg cttcgactcc gcgaagctcc tcggcgggcg gctccggcgc
     1261 gagctcgagg tcgccgccgc ctggctcgtg gacgagccgc cggtcgccgc cgtctgaccc
     1321 ggggaccggt cgtcccgccg gacccggggc ggtcgccgcc gggccggcgc cgcggaggtt
     1381 cgcgcgatcg gcccgcggct gccaagatcg tctggatgcc tgcgaacgaa ggtggcccgc
     1441 ccgacgccgc ggccgtggcc ggggtccgtc gtaccccggg tcaggcgttc tcccgtgcga
     1501 cgggcgccgc ggccgtgctg gccgtcgcgg tcggcgccgt cgggacggtc gccgtccgcc
     1561 actggggccg catcccgttc ggcgtcccga gcaggccctg gtacctgagc tggccgtact
     1621 acctcttctg ggatccccgc ctcgacctcg gctgggcgct cgccgcgctg ccggtcgccg
     1681 cgctcgccgc ggtggtcgtc gtcgccctgg cggccccgcg gacccgcccg gcggccgccc
     1741 cggtccgtgg ggccccggcc gagcctccgg tccgcggggc cccggccgag cctccggtcc
     1801 gtggggcccc ggggccagcg ctctcccggc gggtccggct cggggcgtcc gccgccgccg
     1861 tgttcgtgct ggcgctcgcg gtatccgcgc tcggcggtgg accggccgcc tggcgggcgc
     1921 cgctcgcgta cgccggcgag tacccggacg gcgtgcggca cgtcggcgcg atcccgacgt
     1981 tcctggcccg gttcagcgcg ctgcaacctt ccctgccaga cttcccggcc cagcacccgc
     2041 cgggcgccac ggtgctgtac gcgctggtgg accgggtgtg gccgggcctc accggcgcgg
     2101 cgctcgccac caccgccgcg gcctgcctcg gcgcgctggt ggtcgccggc ctggcccgcg
     2161 acgagctggg ggaggccggc gaccgcctgg cggtcgcctg ctgggcgctc gcgcccatcg
     2221 tcgtgctcta cgcggccacc gcggccgacg cgatgtgggc ccccgtcctg gccggaagcg
     2281 ccctcgccgc ccaccgcggc ctcgaacggc agtccctggc ctggacggtc gccggcggcg
     2341 cgctgctctg ggtcgggtcg atgctcacgt tcggggccgt gctcctcctg cccttcctgc
     2401 tcgtccgggc cctcgcccgc ctcggggcgg gacgggcctg ggtggtgcgc tgggcggtgg
     2461 tcaccgcggc ggtcgccctc ggcctcgccg cgctgtgctg ggccgccacc gggtacgagc
     2521 ccgtctcggc ggtgcgggcg gtcgaccggt tctggcgcgt ggcgcccggg tcgcagcggg
     2581 tctggtggat ctggctcttc ggcgacctga tcgcgttcgg cgggatgctg gggttcccgc
     2641 tgctcgccgc cttcgccgtg cgcgcctgga cggtcgtgcg cgagcgggcc tgggggtcgt
     2701 tcgaggcggc caccctcgcg agcctgctcg ccggcgcggc ctgggggcac accaagggcg
     2761 aggtcgagcg gatgtggcag ttcctggtcc ccttcgcggt ggtcgtcgcc gcgcgccagc
     2821 tcctgcgctg gcgggtgcgc ctcccggtgg tcgccgccct gctcgtcggc caggcactcg
     2881 ccgtgcaggt gctgttcttc acccgctggt agccaggccg ccggggcggc agggggagcg
     2941 gtcgccggct gcccgtcgcg ggctcagccc gtgacggcca gggccgtctc ggtgcccagg
     3001 aaccggccga cgtccgcggc ctccgcctcg aggccgggca ggatcgcgcg gtcgagccgt
     3061 tcgaacggct ccaccgtgac ggtcagccgc cgccccccac gccgctgccg ccaggtgccc
     3121 accacgcggc cgtccacgac cacggccggc tggatgagcc ccccgcccgc ctggatgcgc
     3181 cgggcgaacc gggagggcag cgcgagctcc cggctccggt agccgagcag gtaggcgtcg
     3241 aactgcccga gcagccgcac gcacgggtcc tccaccccgg ggacgtccgg ctcgacgccg
     3301 ccggcgaccc atgcccgctc gccggccacc tccacctcct cgagctcgcc ggtgacgagc
     3361 tcgagcccgc gcctggcctg cccgagcggg atccccgacc aggcggcgag gtcggcggcg
     3421 cccgccgggc cgtaggcgcc cacgtagcgg cgggcgagct cggccagcgc cgcctccagg
     3481 tccagctcgg gccgcgcgcc cacccagtcc ttgagcagca cgtaggtggg ctcgtcgccc
     3541 ggcaggtccg gtccgcggca gaccagcccg cgcatggcgg cgtaggccac gaggtgggcg
     3601 ggggcctggc cctcggcgtc gatggccacg cccttgccgg caagctcgcg caccagctcg
     3661 gccctggtcc gcgggcgccc gccggccagc acctccccga tcgcctcgag cccgcgggcg
     3721 cggacgccct cgtcgagccc gagctgcagc cgccggcgcc ggtcggcggc tgcgaacctg
     3781 ggcccgagca gggcgacgag ccagccc
//
LOCUS       DASPYY010000317         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_54740, whole genome shotgun sequence.
ACCESSION   DASPYY010000317 DASPYY010000000
VERSION     DASPYY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54740"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2416)
                     /locus_tag="VEP73_04880"
     CDS             complement(<1..>2416)
                     /locus_tag="VEP73_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYT25801.1"
                     /translation="QVETGITPLALAQSLDGSVIAVANFGSRSLSIFRTGGGLALLRS
                     LPEVGRPGDRIGVQAGAGLFAAGSQVDLGAGPFPVSYRTPGGEAAAFVVPAGAQREAS
                     LTGVDPLGTRTLALPFRVVDPIGALAPQPTGVQLALAPISCLEGGVGALDALRISPDG
                     GLLALVRNPGGCAGPLELYAADARRPKEFTQALSRADLVPAGHVIGDAAFTSDGRRLW
                     IVDADLSLRIADSDRSSPTFGSLLSVIGFPVFPLSGPGAVATDPLGRYMCAGDMTDDT
                     VRIFRPDGTPVARIPSLGTTDALAASPDGRVLVRAGGGRAEFFNLESLSPIGVSLVHG
                     AKGVGAQHLGVAIPAGGNRAVVRFSNGIGLYNLDPGAGTIGAELYFGNPLSPGVEPGE
                     LVPGPGGGDVLAACAACDTLLRIDAASSPPAISYAPVGQRASRLAASPDGRMLWLARS
                     IDVFTGDVKLLSLSPASALALVSGGGQSGLALSTLPVPVRVRATSGIGSPESGVVVRF
                     ALGSGGALDSDSTTVYKLTDANGEAEVAWRLGAVLGTENLTVTALGVPGGTLTVSAQV
                     VGDDSQIVPAVIEFGPPNGAVNVATGTPVYVRFNQKMQPASVGAKLKLFANGGPVAGT
                     LSFQDGGRLVLDQPTQPLLFAARCSLTVEAGALDTDGQALPASVFATFTVQAPPPLAV
                     TALSPTAGPVGAAVLLNGTGFSSLPGQNTLLFNGTPTTVTSASPTSLGGRVPPGAATG
                     PVTVQVGAAVSPGVLFTVLAPSGPLVDLAGEIAAGVTPTKVAITSDGGRAYVTNPGSN
                     TVTALDL"
BASE COUNT          333 a          881 c          929 g          273 t
ORIGIN      
        1 ggaggtcgag cgccgtcacc gtgttgctgc ccgggttggt gacgtaggcg cggcccccgt
       61 ccgaggtgat ggcgaccttg gtcggggtga cgcccgccgc gatctcgccc gcgaggtcga
      121 cgagcggccc cgacggggcg agcacggtga acaggacccc cgggctcacc gccgcgccga
      181 cctgcaccgt gaccggcccg gtcgccgcac cgggcggcac gcggccgccg agcgaggtcg
      241 gggaggcgga cgtcacggtg gtgggcgtgc cgttgaagag cagggtgttc tggcccggga
      301 gcgagctgaa gccggtgccg ttgaggagca ccgcggcgcc gaccgggccg gcggtcgggc
      361 tgagcgcggt cacggcgagc ggtggcggcg cctgcacggt gaaggtcgcg aacacgctcg
      421 ccggcagcgc ctgtccgtca gtgtcgagcg ctcccgcttc gaccgtgagc gagcagcgcg
      481 ccgcgaacag cagcggctgg gtcggctggt cgagcaccag ccggccgccg tcctggaagc
      541 tgagcgtccc cgccaccggg ccgccgttcg cgaacagctt gagcttggcc ccgacgctgg
      601 cgggctgcat cttctggttg aagcggacgt agaccggggt cccggtggcg acgttcaccg
      661 cgccgttcgg cgggccgaac tcgatcaccg ccgggacgat ctggctgtcg tcccccacca
      721 cctgggcgct caccgtgagc gtgccgcccg ggactccaag cgccgtgacc gtgaggttct
      781 cggtgccgag cacggctccg agcctccagg ccacctcggc ctcgccgttc gcgtcggtga
      841 gcttgtagac cgtggtgctg tcggaatcga gtgccccgcc ggagccgagc gcgaagcgca
      901 ccaccacgcc ggactcgggg ctgccgatcc cgctggtcgc gcgcacccgc accgggacgg
      961 ggagcgtcga gagggcgagc ccggactggc cgccgcccga aaccagggcg agcgccgagg
     1021 ccggggagag gctcaggagc ttgacgtccc ccgtgaagac gtcgatcgag cgggcgagcc
     1081 acagcatgcg tccgtcgggc gaggccgcga gccggctcgc gcgctgcccg accggggcgt
     1141 agctgatcgc gggcgggctc gacgcggcgt cgatccggag cagcgtgtcg cacgccgcgc
     1201 aggcggcgag cacgtcgccg ccgcccggtc ccggcaccag ctcccccggc tcgacgccgg
     1261 gcgacaaggg attgccgaaa tagagctcgg ccccgatggt accggccccg ggatcgagat
     1321 tgtagagccc gatcccgttc gagaaccgca ccaccgcgcg gttcccgccg gccgggatcg
     1381 cgacgccgag gtgctgcgcg ccgacgccct tcgcgccgtg cacgagcgag acgccgatcg
     1441 ggctcaggct ttccaggttg aagaactcgg cgcgaccgcc gcccgcccgg accagcacgc
     1501 gaccgtcggg cgaggccgcg agcgcgtcgg tggtcccgag ggaggggatt cgggcgaccg
     1561 gggtgccatc gggcctgaag atccgaaccg tgtcgtccgt catgtcgccg gcgcacatgt
     1621 agcgcccgag cggatcggtc gccacggcgc ccggccccga gagcgggaac accgggaagc
     1681 cgatcaccga gagcagcgag ccgaaggtcg ggctcgagcg gtcgctgtcc gcgatccgga
     1741 ggctcaggtc ggcgtccacg atccacagcc ggcggccgtc cgaggtgaag gccgcgtcgc
     1801 cgatgacgtg cccggcgggc accaggtcgg cacgcgagag cgcctgcgtg aactccttcg
     1861 ggcggcgggc gtcggccgcg tagagctcga gcgggcccgc gcacccgccc gggttccgca
     1921 ccagcgcgag cagtccgccg tcgggcgaga tccggagcgc gtcgagggct ccgacgccgc
     1981 cctcgaggca ggagatcggc gcgagcgcga gctggacccc ggtcggctgc ggcgccagcg
     2041 cgccgatcgg atccaccacc cggaacggca gcgcgagcgt gcgcgtcccg agcggatcca
     2101 ccccggtgag cgacgcctca cgctgcgccc cggccgggac cacgaacgcg gccgcttcgc
     2161 cgcccggcgt gcggtaggag accgggaacg gcccggcacc gaggtccacc tggctcccgg
     2221 ccgcgaacag tcccgcgccc gcctggacgc cgatgcggtc cccgggccgc ccgacctcgg
     2281 gcagcgagcg cagcagggcg aggccgccgc cggtgcggaa gatcgagagg ctgcgcgagc
     2341 cgaagttcgc gaccgcgatg acgctcccat ccaggctctg cgcgagcgcg agcggcgtga
     2401 tgccggtctc gacctg
//
LOCUS       DASPYY010000318         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_617708, whole genome shotgun sequence.
ACCESSION   DASPYY010000318 DASPYY010000000
VERSION     DASPYY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617708"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VEP73_04885"
     CDS             complement(<1..519)
                     /locus_tag="VEP73_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYT25802.1"
                     /translation="MGDRPLAVVPGGRVPVWLWAQADEVEPQALAQLARVSALPWVFH
                     HVAAMPDVHLGKGATIGSVIAMVDAVAPSAVGVDIGCGVAAVRTRLLDRDLPDDLGRV
                     RAAIEAAVPVGFQGHKEPPRRGTPQWDGWAALEGRYGSLDASVQTPKQLERSACQMGT
                     LGGGNHFIELCLD"
     gene            690..>1525
                     /locus_tag="VEP73_04890"
     CDS             690..>1525
                     /locus_tag="VEP73_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HYT25803.1"
                     /translation="MSANPNAEDLLRQLAPQVLGALVRRYGHFDTCEDATQEALLAAA
                     VQWPDQGVPASPRAWLITVASRRLTDQLRSEQARRRREATAATLVPPGELVAPAADDE
                     RAPAEDDTLTLLFLCCHPALSAVSQVALTLRAVGGLTTAEIARAFLVPGATMAQRISR
                     AKQRIKASGAPFRLPPEPERADRLGVVLQVLYLIFNEGYTATSGPDLQRDELTAEAIR
                     LTREVRRQLPADGEVAGLLGLTLLTEARRPARSRPDRSLVPLAEQDRSRWNRKFIDEG
                     LAL"
BASE COUNT          202 a          595 c          540 g          188 t
ORIGIN      
        1 gtccaggcac agctcgatga agtggttgcc cccgccgagc gtccccatct ggcaggccga
       61 gcgctcgagc tgcttcgggg tctggacgct cgcgtcgagc gacccgtacc gtccctcgag
      121 cgccgcccac ccgtcccact gcggggtgcc cctccgcggc ggctccttgt gcccctggaa
      181 ccccaccggg acggcggcct cgatcgccgc gcggacccgc ccgaggtcgt cgggcaggtc
      241 ccggtcgagg agccgcgtgc gcaccgcggc gacaccgcag ccgatgtcga ccccgacggc
      301 gctcggggcg accgcgtcga ccatggcgat gaccgagccg atggtcgcgc ccttgccgag
      361 gtggacgtcg ggcatggcgg cgacgtggtg gaagacccag ggcagggcgg agacccgcgc
      421 gagctgggcg agcgcctggg gctcgacctc gtccgcctgc gcccacagcc acaccggcac
      481 cctgccgccc ggcaccacgg ccagcggtcg gtctcccatg ctccctcctc gaccttcgcg
      541 ggtgacctgt tgagcgtcgt gggtgccaag gctaccgacg gactcgtcag ccgcgcgcct
      601 cgagcagctg ccacgccggc cgtcggtgcg ggtagcttct gcacgatgag atcacctggg
      661 cacacgagac cgggccggcg tgcggtggtg tgagcgccaa ccccaacgcc gaggacctgc
      721 tgcgccagct ggcgccgcag gtcctcggcg cgctcgtccg gcgctacggc cacttcgaca
      781 cgtgcgagga cgcgacccag gaggcgctgc tggccgccgc cgtccagtgg cccgaccagg
      841 gcgtaccggc cagcccgcgg gcctggctga tcacggtcgc gtcccgccga ttgaccgacc
      901 agctgcgcag cgagcaggcg cgccggagga gggaggccac cgccgcgacc ctggtgccgc
      961 ccggcgagct cgtggcgccg gccgcggacg acgaacgcgc gccggccgag gacgacaccc
     1021 tgaccctgct gttcctgtgc tgccacccgg cgctgtcggc ggtctcccag gtcgcactca
     1081 ccctgcgcgc cgtcggcggg ctgaccaccg cggagatcgc ccgtgcgttc ctcgtgcccg
     1141 gggcgaccat ggcccagcgc atcagccggg ccaagcagcg catcaaggcc agcggcgccc
     1201 cgttccgcct gcccccggag cccgagcggg ccgaccggct tggcgtggtc ctgcaggtgc
     1261 tgtacctgat cttcaacgag ggctacaccg ccacgtccgg tcccgacctg cagcgcgacg
     1321 agctgaccgc agaagcgatc cgcctcaccc gagaggtccg ccgacagctt cccgcggacg
     1381 gcgaagtcgc gggcctgctt gggctgacgc tgctgaccga ggcgcggcgg cccgcgcgca
     1441 gccgccccga ccgcagcctc gtcccgctcg cggaacaaga ccggagccgg tggaaccgga
     1501 agttcatcga cgagggtctc gcgct
//
LOCUS       DASPYY010000319         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1038897, whole genome shotgun sequence.
ACCESSION   DASPYY010000319 DASPYY010000000
VERSION     DASPYY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038897"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1769
                     /locus_tag="VEP73_04895"
     CDS             <1..>1769
                     /locus_tag="VEP73_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25804.1"
                     /translation="VGEDRTGRVYAHPATSPLVNQTFFAGLPYTSFGAENLTGRISTT
                     RLRAPRAVALNDSGGVIFSDEGHDRRLFEAYPASGIVRAYGIYPDGVGTHQPAAAVFD
                     PVRHNVVASDFGGGRLLRFLGTGGFATIIAGLGTVAGDTTVAFASVGTPAEIALAPGG
                     DIFFADPSSHRVRKLTGDGHVVTVIGTGVQGAGGDGSGAGTQLDRPSGVALDPATQRL
                     YVGDTGNDRVLAFDLGAAGVPLLSTTAVPAPGSLALTHALGGALLIASGNQVLELALG
                     GAGAVVAAGGGGYGPAAGANLGTQQVLLAPSGLTPDGADGLLFADPLLDLVFHLQANG
                     QIAVAAGVPAGMTMGSQALWFGADAASDPAEVAGWVQPAGYGNNWSQRVLSPPISLAA
                     HPHARLSFDAALDLGEGAAVQPVSGGEFVLVQALKTDGGWTSLAVRLLGGGAPSFPIR
                     SFAGRGLVHVTADLDTDGNDLLGLAAPTRLRLVVQTSERGSNEDGRTANTRGAAVVDN
                     LHVEDPGGPVVAPTDFEAGGTGDWTLAALNGVTLEQDCLSPASAAGPADPGPVQLNCP
                     PYTRDLAPPNTVVQLSPGFDAQD"
BASE COUNT          217 a          671 c          647 g          234 t
ORIGIN      
        1 ggtgggcgag gaccgcactg gacgtgtcta cgcgcatccg gcgacgagcc cgctggtcaa
       61 ccagacattc ttcgccggcc ttccgtatac gagcttcggc gcggagaacc tcaccggccg
      121 gatctcgacc acccggctga gggcgccgcg cgcggtggcc ctgaacgact cgggcggcgt
      181 gatcttcagc gacgaaggcc acgatcggcg gctgttcgag gcctatcccg cgagcggcat
      241 cgtgcgcgcc tacggcatct atcccgacgg ggtcggaacc caccagccgg cggcggccgt
      301 cttcgatccg gtccggcaca acgtggtcgc gagcgacttc ggaggcggga ggctgctgcg
      361 attcctgggc acgggtggtt tcgcgaccat catcgcgggg ctcggcaccg tcgccgggga
      421 cacgacggtc gcgttcgcga gcgtcggcac gccggccgag atcgcgctcg cgccgggcgg
      481 agacatcttc ttcgccgatc cctcgagcca ccgggtgcgc aagctcaccg gggacggcca
      541 cgtggtcacg gtgatcggga ccggcgttca gggcgcggga ggcgacggca gcggcgcggg
      601 cacccagctc gaccgcccgt cgggcgtggc gctcgacccc gcgacgcagc gcctctacgt
      661 gggcgacacc gggaacgacc gcgtgctggc gttcgacctc ggggccgcgg gcgtgccgct
      721 cctctccacc accgcggtgc ccgcgccggg gtcgctcgcg ctcacgcacg ccctgggcgg
      781 cgcgctgttg atcgcgagcg ggaaccaggt gctcgagctc gcgctcggcg gtgcgggcgc
      841 ggtggtggcg gcgggcggcg gcggctacgg cccggccgcg ggtgccaacc tcggcaccca
      901 gcaggtgctg ctcgccccct ccggcctcac gcccgacggc gcggacggcc tcctgttcgc
      961 cgatcccctg ctcgacctgg tcttccacct ccaggccaac gggcagatcg cggtcgcggc
     1021 gggtgtgccg gccggcatga ccatgggaag ccaggccctg tggttcgggg ccgacgcggc
     1081 ctccgatccc gccgaggtcg caggctgggt ccagcccgcg ggctacggga acaactggag
     1141 ccagcgcgtg ctctcgcccc cgatctcgct cgccgctcac ccgcacgccc ggctctcgtt
     1201 cgacgccgcg ctcgacctgg gcgaaggcgc ggcggtgcag ccggtcagcg gcggcgagtt
     1261 cgtcctcgtc caggccctca agaccgacgg cggctggacg tcgctcgccg tacgcctgct
     1321 cggcggcggg gcgccgagct tcccgatccg gtccttcgcg ggccgtgggc tcgtgcacgt
     1381 caccgcggat ctcgatactg acgggaacga cctgctcggc ctcgcggccc cgacgcggct
     1441 ccgcctcgtg gtccagacca gcgagcgggg ctcgaacgag gacggccgca ccgccaatac
     1501 gcgcggcgcg gcggtggtgg acaacctgca cgtcgaggac cccgggggcc cggtggtcgc
     1561 gcccacggac ttcgaggcgg gcgggaccgg cgactggacg ctcgcggcgc tcaacggcgt
     1621 gaccctcgag caggactgcc tctcgccggc ctccgccgcg ggcccggccg acccgggccc
     1681 ggtgcagctg aactgcccgc cctacacgcg cgacttggca ccgcccaaca ccgtggtcca
     1741 gctctccccg ggcttcgacg cccaggatc
//
LOCUS       DASPYY010000320         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_478746, whole genome shotgun sequence.
ACCESSION   DASPYY010000320 DASPYY010000000
VERSION     DASPYY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478746"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VEP73_04900"
     CDS             <1..1118
                     /locus_tag="VEP73_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HYT25805.1"
                     /translation="AADIYAVTGQQFLPFNTLCQLVAARATPQLEAAARLLQIPDLFA
                     YWLTGVEGAEITNASTTQLLDVTARTWAADLIRRLGLAPGLFPPLREPGDPASELLPS
                     VLEAVGLAGQGPVPVTAVGSHDTASAVAGVPADGERFAYISCGTWALVGVELDQPVLS
                     EESRQANFTNELGVDGTIRYLRNVMGLWLLQESLRVWSDADGGRPVDLEALLREAAGL
                     PAFCAVIDVDDPVFVPPGDLPARIAATCERTGQTAPQSPAETVRCVVDSLALAFRRTL
                     HDAQRLSGRDVEVIHMVGGGARNELLCQLTADVCELPVVAGPAEATALGNLLIQARAQ
                     RAAPADLPGLRSLLRQTQSLRTYLPRRRGRPTPEVPG"
     gene            1115..>3191
                     /locus_tag="VEP73_04905"
     CDS             1115..>3191
                     /locus_tag="VEP73_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HYT25806.1"
                     /translation="MTLEPEPELKAKLAALTLEQKVRLLTGAGFWSLHPEPAIGLRRV
                     VVSDGPAGVRGERWDERDPSANVPSPSALAATWDEARVERLGRLLAAEARRKGVQVLL
                     APTVNLHRSPYGGRHFECFSEDPLLTARIGAAYVRGVQSGGIAATVKHFVANDSETER
                     HTVDVRVDERTLRELYLAPFEAIVRDAGVWAVMAAYNGVNGTTMTESPLLREILHAEW
                     GFDGVVMSDWFGTRSTVPSGRSGLDLVMPGPSGPWGDDLVAAVSDGHVSEAAIDDKVL
                     RLLRLAARVGALDLDLGADRLEAEREPWSSAAVAVELRATAAAGFVLVRNEHARDHDG
                     ESERAVLPLEAGRLRRVAVLGPNAAVPRTLGGGSATVFPPYAISPLDGLRVALEPEVE
                     VAHAPGARATTGIPPADLGLLRPPASSPEGEEGGVELRFVAADGTVLHREHRRTAAFT
                     WLGSFGDFPAERVAAIEVRTRLLADASGDWTVGCSGVGDFELSLAGTPRFAGPLAPEP
                     GVEPIGAMMRPPQHLVRVPLEAGQQLPITLSYRLTDASRIPAFQLNVDRPCGSDEEEL
                     ARAVALARDADVAVVVVGTTDEVESEGFDRNSLALPGRQDDLVRAVAAANPHTVVVVN
                     AGAPVLLPWADEVPAILLTWFPGQEFGNALADVLLGASEPGGRLPTTWPASPDGLPTT
                     TPAAGRLDYA"
BASE COUNT          386 a         1264 c         1111 g          430 t
ORIGIN      
        1 ccgccgccga catctacgcc gtcaccggcc agcagttcct gccgttcaac acgctctgcc
       61 agctggtggc cgccagggcg actcctcagc tggaggcggc ggcgcggctg ctgcagatcc
      121 ccgacctgtt cgcctactgg ctgactgggg tggagggggc cgagattacc aatgcctcca
      181 ccacccagct cctcgacgtg accgcccgca cctgggctgc cgacctgatc cgccggctcg
      241 gcctggctcc cggcctgttc ccgccgctgc gcgaaccggg cgaccccgcc agcgagctgc
      301 tacccagcgt gctggaggcc gtcgggctcg ccggccaggg ccccgtgccg gtgaccgccg
      361 tggggtcgca cgacaccgcc tccgccgtcg ccggggtccc cgccgacggc gagcgcttcg
      421 cctacatctc ctgcggcacc tgggcgctgg tcggcgtcga gctggaccag ccggtgctca
      481 gcgaagagag ccggcaggcc aacttcacga acgagctggg ggtggacggc accatccgct
      541 atctccgcaa cgtcatgggc ctctggctgc tgcaggagtc gctgcgcgtc tggtcggacg
      601 cggacggagg gcggcccgtc gacctcgagg cgctgctgcg cgaggcggcc ggcctcccgg
      661 cgttctgcgc ggtgatcgac gtggacgacc cggtcttcgt cccaccgggc gacctccccg
      721 cgcggatcgc cgccacgtgc gagcgcaccg gccaaacagc accgcagagc ccggccgaga
      781 ccgtccgctg cgtcgtggac agcctcgcgc tggcgttccg ccggaccctc cacgacgcgc
      841 agcggctctc gggccgcgac gtcgaggtca tccatatggt cggcggcggc gctcgcaacg
      901 agttgctgtg ccagctcacc gcggacgtct gcgagctgcc ggtggttgcc gggccagccg
      961 aggcgaccgc cctcggcaac ctgctgattc aggcgcgggc gcagcgcgcc gccccggccg
     1021 acctgcccgg cctgcgctcc ctcctgcggc agacccaatc cctgcggacc tacctcccgc
     1081 gcaggcgcgg gcgacccacc ccggaggtgc ctggatgacc ctggagccgg agccggagct
     1141 caaggccaag ctggcggcgc tcaccctgga gcagaaggtg cggctgctca ctggcgccgg
     1201 tttctggtcg ttgcacccgg aaccggccat cgggctgcgg cgggtggtgg tctccgacgg
     1261 cccggctggc gtgcgcggcg agcgctggga cgagcgcgac ccgtccgcga acgtgccgtc
     1321 ccccagcgcc ctcgccgcca cctgggacga ggcccgggtc gagcgcctcg gtcgtctcct
     1381 ggccgccgag gccaggcgca agggcgtcca ggtgctgctc gccccgaccg tgaacctgca
     1441 ccgcagcccc tacggcggcc gccacttcga gtgcttcagc gaggatccgc tgctgaccgc
     1501 gcggatcggc gccgcctatg tgcgcggcgt ccagtccggc gggatcgcgg cgacggtgaa
     1561 gcacttcgtc gccaacgact ccgagaccga gcggcatacc gtggatgtgc gcgtcgacga
     1621 gcgcaccctg cgtgagttgt acctggcccc cttcgaggcg atcgtgcgcg acgcgggggt
     1681 ctgggcggtg atggcggcct acaacggtgt caacgggacc acgatgaccg agagcccgct
     1741 gctccgggag atcctccacg cggagtgggg cttcgacggc gtggtgatgt ccgactggtt
     1801 cggcacccgc tcgaccgtcc cgagcggtcg cagcggcctc gacctggtta tgcccggccc
     1861 ctcgggcccg tggggcgacg acctggtcgc ggcggtcagc gacggtcacg tcagcgaggc
     1921 ggccatcgac gacaaggtgc tgcgcctgct gcgcctggcc gcccgcgtcg gcgccctcga
     1981 cctcgacctc ggcgccgatc ggctggaggc ggagcgggaa ccctggtcca gcgccgcggt
     2041 cgccgtcgag cttcgcgcca ccgccgccgc gggattcgtc ctggtccgca acgagcacgc
     2101 ccgcgaccat gacggggaga gcgagcgcgc cgtgctgccc ctggaagcgg gccgcctgcg
     2161 ccgggtggca gtgctggggc cgaacgcggc cgtcccccgc accctcggcg gtggtagcgc
     2221 cacggtcttc ccgccctatg cgatctcccc gctcgacggc ctccgcgtcg ccctggagcc
     2281 cgaggtcgag gtcgcccacg cgccgggagc gcgggccacc accgggatcc cacccgccga
     2341 cctgggcctg ctgcggcctc cggcgtcatc cccggagggg gaggagggcg gtgtcgagct
     2401 gcgcttcgtc gccgccgacg gcaccgtgct gcaccgcgag caccgccgca ccgccgcctt
     2461 cacctggctg ggctcgttcg gcgatttccc tgccgagcgg gttgccgcca tcgaggtgcg
     2521 tacccggctg ctggccgacg ccagcggcga ctggaccgtc ggctgctccg gtgtcgggga
     2581 cttcgagctg tcgctcgccg ggacgccccg gttcgccggc ccgctggccc ccgaacccgg
     2641 cgtggagccg atcggcgcca tgatgcgccc gccccagcac ctggttcggg tcccgctgga
     2701 ggctggccag cagctcccga tcaccctcag ctaccgcctc accgacgcca gccgaatccc
     2761 cgccttccag ctcaacgtgg accgcccctg cggcagcgac gaggaggagc tcgcccgcgc
     2821 ggtggccctg gcccgcgacg ccgacgtcgc cgtggtggtc gtcggcacca ccgacgaggt
     2881 cgagagcgaa ggcttcgacc gcaactccct ggccctcccc ggccgccagg acgacctggt
     2941 ccgcgcggtc gccgccgcca acccccacac cgtcgtggtg gtcaacgccg gcgcccccgt
     3001 cctgctcccc tgggccgacg aggtccccgc gatcctgctc acctggttcc ccggccagga
     3061 gttcggcaac gccctcgccg acgtcctcct cggcgccagc gaaccaggtg gccgcctccc
     3121 caccacctgg cccgcctccc cggacggcct ccccacaacc accccggccg ccggccgcct
     3181 ggactatgcc g
//
LOCUS       DASPYY010000321         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1039156, whole genome shotgun sequence.
ACCESSION   DASPYY010000321 DASPYY010000000
VERSION     DASPYY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039156"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VEP73_04910"
     CDS             complement(<1..491)
                     /locus_tag="VEP73_04910"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HYT25807.1"
                     /translation="MDFTHYSDRSVALAVALVNGGDRLDLAGLGRLVRGFQVRPVAAD
                     VPAARALAARLRAAFGAAEPAEAVATINAVLAEAGPVPQVSGHGGQPWHLHVVPADAA
                     FTRWLAATTAMALAGVVCHFGAARLGCCAAADCEDAFVDTSRNRSRRYCSDACANRSN
                     VAA"
     gene            562..711
                     /locus_tag="VEP73_04915"
     CDS             562..711
                     /locus_tag="VEP73_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25808.1"
                     /translation="MHDIPCPLHGAAAAPDPRQRLSTHDTSLGTVVYYRCHCGRPALA
                     TLGRA"
     gene            complement(772..972)
                     /locus_tag="VEP73_04920"
     CDS             complement(772..972)
                     /locus_tag="VEP73_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25809.1"
                     /translation="MPRRHSALIVALVGLPVAGPTDQPLDDPLTAGTLQLPASLAPHL
                     PLAGRMATRRAARNRSRTFTAR"
     gene            complement(1108..1209)
                     /locus_tag="VEP73_04925"
                     /pseudo
     CDS             complement(1108..1209)
                     /locus_tag="VEP73_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1242..1925
                     /locus_tag="VEP73_04930"
     CDS             1242..1925
                     /locus_tag="VEP73_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25810.1"
                     /translation="MATRARGEGPTAVYLEVGKKRVFACALDWPGWCRAGKDEQLALE
                     ELAAYAPRYAVVAEQAKIAFPDRTAEGLKVVERLPGSSTTDFGAPGEIAGADAKRATG
                     DQAERLAALVTASWAVFDRVRAGAPAKLRKGPRGGGRDRDKMVDHVLGAEAAYARKLG
                     VKHRQPAIDDEAAIAALRDDIVEVLRVPSDGTPPVPKGWPPRYAARRIAWHALDHAWE
                     MEDRSDPDG"
     gene            2249..>2556
                     /locus_tag="VEP73_04935"
     CDS             2249..>2556
                     /locus_tag="VEP73_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25811.1"
                     /translation="MRTPLVTSPLADLRARLPDLMLRDQHRLRRRVDGARKIGDPEAR
                     QAAADAITADVDAAALRVARRRAGVPAVSYPPALPISQRRDEILAAVRDHQVVIVAGE
                     T"
BASE COUNT          336 a          955 c          957 g          308 t
ORIGIN      
        1 caggcggcca cgttgctgcg gttggcgcag gcgtccgagc agtagcggcg cgagcggttt
       61 cgcgacgtgt ccacgaacgc gtcctcgcag tcggcggcgg cgcagcagcc gagccgcgcg
      121 gcgccgaagt ggcagaccac cccagccagg gccatcgccg tggtcgcggc cagccagcgg
      181 gtgaacgccg cgtcggccgg caccacgtgc aggtgccagg gctggccgcc gtgcccgctg
      241 acctgaggca cggggccggc ctcggcgagg acggcgttga tcgttgcgac ggcctcggcc
      301 ggctcggcgg cgccgaacgc ggcccgcagg cgggcggcca gagcgcgcgc ggcgggcacg
      361 tcggccgcga cagggcggac ctggaagccg cgcacgagcc ggcccaggcc ggccaggtcg
      421 agccggtcgc cgccgttcac gagcgcgacc gcgagcgcca ccgagcggtc gctgtaatgc
      481 gtgaagtcca tttgacttct tactctacgt gcgctagtgt gtaagctgca agcagaagtt
      541 cgaccattac ggaggctcac ggtgcacgac atcccctgcc ccctgcacgg cgccgccgcc
      601 gcccccgacc ctcgtcagcg gctctccacc cacgacacgt cgctcgggac ggtcgtctac
      661 taccgctgcc actgcggacg ccccgccctg gccacgctcg ggcgcgccta ggctagctgc
      721 catctcgctg catcagcgca tggctccgct ggcgggccgg ctgctcctcg ctcacctggc
      781 ggtgaaggtg cgcgaccggt tgcgggccgc gcgccgggtc gccatgcggc cggcgagcgg
      841 caggtggggc gccaggctgg cgggcaattg cagcgtgccg gcggtgagcg ggtcgtcgag
      901 gggctggtcg gtcggcccgg cgacgggcaa gccgaccagg gcgacgatca gcgccgagtg
      961 gcggcgtggc accgcaggtc ccgccgggcc atccgccaca gcgccggcgg ccaccgcttc
     1021 ctcatgtgac cacctcaggg ttgcggggct catgcaggtc gtccgctggt gcggtctggt
     1081 cgatcatgcg accgtcgcgc aggaagacga cgcggtcggc tcgtccgcga gcaggaggcg
     1141 ccgttcgccc atcacggcgc gagcgatcgc gacgcgctgc tcgccgccgg acagctcctc
     1201 gggggacctg ccgtcgatcg aggccgtgag atactcaggc catggcgacc agggcacgcg
     1261 gggaggggcc gacggcggtc tacctcgagg tgggcaagaa gagggtcttc gcttgcgccc
     1321 tcgactggcc gggctggtgc cgcgccggca aggacgagca gctggcgctg gaggagctgg
     1381 cggcgtacgc gcccaggtac gcggtcgtgg ccgagcaggc caagatcgcg tttcccgacc
     1441 ggacggcgga gggcctgaag gtcgtcgagc ggctgccggg gtcgtcgacc accgacttcg
     1501 gcgctcccgg ggagatcgcc ggcgccgacg ccaagcgcgc gaccggcgac caggccgagc
     1561 gtctcgccgc gctggtcacg gcgtcgtggg cggtgttcga ccgggtccgc gccggcgcgc
     1621 ccgccaagct gcgcaagggc ccccgcggcg gcggtcgcga tcgggacaag atggtcgacc
     1681 acgtcctcgg cgcggaagcg gcctacgccc gcaagcttgg cgtcaagcac cgccagcccg
     1741 ccatcgacga cgaggccgcc atcgccgcgc tgcgcgacga catcgtcgag gtgctgcgcg
     1801 tgccctcgga cggcacgccg ccggtgccca agggctggcc gccccgctac gcggctcgcc
     1861 ggatcgcctg gcacgccctg gaccacgcct gggagatgga agaccgcagc gaccccgacg
     1921 gctgacgcgg gccgagcggg ccgcctcgga ggcgggctgg agcgcctccc tcgccccggc
     1981 cgaacgagcc gccgagctcc tcgacgcgga ccgcccggtg gccgcggcct gggatccccg
     2041 cctgaccgag cgatgcggtc gctcggcgtc aaggtcgagg aggagcgcgt gctcgccgac
     2101 ctgctcgccg acctcgccac ctgaaccgga ggctgggacg ggccgggacg ggccgggaat
     2161 attcgcttcc ccttggacgc ttgcatcccg gacccttgaa cctggggcgg accccgcgtc
     2221 gccccttgcc cgacgcctcg ccattcgtat gagaacgccg ctcgtgacct ctccgctcgc
     2281 cgacctgcgg gcgcgcctgc ccgacctgat gctccgcgac cagcaccgcc tgcggcgccg
     2341 ggtcgacggc gcccgcaaga taggcgatcc cgaggccagg caggcggccg ccgacgcgat
     2401 cacggccgac gtcgacgcgg ccgcgctgcg cgtcgcgcgg cgacgcgccg gcgtgcccgc
     2461 ggtcagctac ccgccggccc tcccgatcag ccagcgcagg gacgagatcc ttgccgcggt
     2521 ccgcgaccac caggtcgtga tcgtcgccgg cgagac
//
LOCUS       DASPYY010000322         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_336377, whole genome shotgun sequence.
ACCESSION   DASPYY010000322 DASPYY010000000
VERSION     DASPYY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336377"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1137
                     /locus_tag="VEP73_04940"
     CDS             13..1137
                     /locus_tag="VEP73_04940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYT25812.1"
                     /translation="MGGALLLAALAGCSRDSSPPTSTTGTPATRPPATRPPGAAGTGP
                     ASTRPPVGGGPRQPTGAPVTLAFGGDVNFEGVTGARLARDPATAMGPITRVLRQADLA
                     MVNLETAVTTRGTPAAGKEFTFRTPPTAFAALRSSGVDVVTMANNHGEDYGPVGLRDS
                     LAAAAAARFPVVGIGRDAEQAFRAWLFTVRGQRIAVLGATQVLDSSLAAAWSAGDRKP
                     GLASAYQTERLLAAVRAARRSADTVVVFLHWGQELSSCPIARQRAIAPRLVAAGADVV
                     VGSHAHVLAGGGFLDGAYVDYGLGNFVFYSSGGATARTGVLTLTVRGRAVTGVRWTPA
                     TISGGVPLPLSGAAARQALASWRALRGCTGLQAEAAVGAG"
     gene            complement(1362..1493)
                     /locus_tag="VEP73_04945"
     CDS             complement(1362..1493)
                     /locus_tag="VEP73_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25813.1"
                     /translation="MAASGLGVRWWLENDLPYSAEEMDELYRRLTEPGIRAGLRPKA"
     gene            1510..2340
                     /locus_tag="VEP73_04950"
     CDS             1510..2340
                     /locus_tag="VEP73_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT25814.1"
                     /translation="MIYGLVKAGSDGWSADATLIPLAAGLLLAGVFVAIERAAPEPLV
                     PLGLLARRSLVAGQLVMLSASGLLLATYFLSSLYLQRLLDFTALQTGLVFLPAALVTI
                     AGSHLGARAIGRLGPRPVAAVGFGLAAAGALLLARLPADATVLVDLLPGFLLTTAGVG
                     ATFVVAATTAMAHVGQARAGVTSGLLNTGHELGASLGWRWSPRSPAPASPPIPWPGGR
                     RPPGSTARSWLRGGRRCCRPAARPPPTGPSSPTDHRPHHERNAARSSPLLQERKVAGW
                     "
BASE COUNT          265 a          987 c          881 g          348 t
ORIGIN      
        1 cgcgcgtgcg ccatcggcgg cgcgctcctg ctcgccgcgc tcgccggctg ctcgcgggac
       61 tcctccccgc cgacgtcgac gacgggcacg ccggcgacca ggccaccggc gaccaggccg
      121 cccggagcgg ccgggaccgg accggcctcc acccgaccgc ccgtcggcgg ggggccgcgc
      181 cagccgaccg gcgcccctgt cacgctcgcc ttcggcggcg acgtgaactt cgagggggtg
      241 accggtgctc gcctggcacg cgacccggcc acggcgatgg gcccgatcac ccgggtgctc
      301 cgccaggccg acctggccat ggtcaacctc gagacggcgg tgacgacgag gggcaccccg
      361 gccgccggga aggagttcac cttccgcacc ccgccaaccg cgttcgccgc cctgcgatcc
      421 tcgggcgtcg acgtggtgac catggccaac aaccatggcg aggactacgg acctgtcggg
      481 ctgcgggact ccctggcggc cgccgcggcg gcccgcttcc cggtcgtcgg catcggccgg
      541 gacgccgagc aggcattccg ggcgtggctg ttcactgtcc ggggccagcg gatcgcggtg
      601 ctcggcgcca cccaggtcct cgactcgagc ctggccgccg cctggagcgc cggcgaccgc
      661 aagccggggc tcgcctccgc ctaccagacc gagcggctcc tcgccgccgt gcgggcggcc
      721 aggcgctccg ccgacaccgt ggtggtgttc ctgcactggg gccaggagct gtcgagctgc
      781 ccgatcgcga ggcagcgggc catcgccccc cgcctggtcg cggccggggc cgacgtcgtg
      841 gtcggctccc acgcgcacgt cctggccggg ggcggcttcc tcgacggcgc ctacgtcgac
      901 tacgggctcg gcaacttcgt cttctactcc tcgggcggtg ccaccgcgcg gaccggcgtg
      961 ctcaccctga ccgtccgcgg gcgtgccgtc accggcgtcc gctggactcc ggcgaccatc
     1021 tccggcggcg tgcccctgcc cctgtccggt gccgccgcca ggcaggcgct cgcatcctgg
     1081 cgggcactgc gcggctgcac cggcctccag gcggaggccg ccgtcggcgc cggctgatcg
     1141 cctcatcatg atggccgtcg agcggttcga cctcgtcgtg gtcggtgccg ggctcgggcg
     1201 gcacttcccc ggcgacaggg acctgggcgg ccaggcggga cgggtggacc ctacggttgt
     1261 gggcagggcc tcaccgggag ttccgctggc gagccggtca acgcccgtac ctcactctcc
     1321 gagcctgcag gaggcacggc agcgggcaag gagacctcgc ttcacgcctt cgggcgcagg
     1381 ccggcacgga tcccgggctc ggtcagccgg cggtacagct cgtccatctc ctccgccgag
     1441 tacggcagat cgttctcgag ccaccaccgc acgccgagcc cgcttgccgc cacggcggcc
     1501 atcggcgcgg tgatctacgg gctggtcaag gcgggcagcg acggctggag cgccgacgcc
     1561 accctgatcc cgcttgccgc ggggctgctg ctggccgggg tcttcgtcgc catcgagcgg
     1621 gcggcccctg agccgctggt gcccctgggg ctgctggccc ggcggtcgct ggtggccggc
     1681 cagctggtga tgctgtcggc ctccggcctg ctgctggcaa cctacttcct gtcctccctg
     1741 tacctgcagc gcctgctcga cttcaccgcg ctccagaccg gcctggtctt cctgccggcg
     1801 gcgctggtca ccatcgccgg cagccacctc ggcgcccgcg ccatcgggcg tctcggtcct
     1861 cggccggtcg ccgcggtcgg gtttggtctg gccgcagcgg gcgccctgct gctggcacgc
     1921 ctgcccgccg acgcgaccgt gctggtcgac ctgctccccg gcttcctgtt gaccaccgcg
     1981 ggggtgggcg cgaccttcgt ggtcgccgcc accaccgcca tggcccacgt cggccaggcc
     2041 cgggccggcg tgacctcggg gctgctcaac accggccacg agctcggcgc ctccctgggg
     2101 tggcgttggt ctccgcgatc gccggcgcca gcgtcgccac cgatcccctg gccgggcggc
     2161 cgccggccgc cgggttcgac cgcgcgttcc tggctgcgcg gtggtcgccg ctgctgccgc
     2221 ccggccgccc gcccgccacc gacgggcccg tcttcgccca ctgaccaccg cccgcaccac
     2281 gaacgcaacg ccgctcggag cagtcccctg ctccaagagc ggaaggtcgc tggatggtga
     2341 ggaaccgctc cgccgacgga tgccgtgctg ccgtatatca cctgggtcat gctcgacagc
     2401 gcgggagcgg gcggcgcgag cccggcgcaa cccggtcacc aaacccctga cagtgttcgt
     2461 cgacgacgtt gatgcgcact t
//
LOCUS       DASPYY010000323         5286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2162620, whole genome shotgun sequence.
ACCESSION   DASPYY010000323 DASPYY010000000
VERSION     DASPYY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162620"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1342)
                     /gene="pstS"
                     /locus_tag="VEP73_04955"
     CDS             complement(233..1342)
                     /gene="pstS"
                     /locus_tag="VEP73_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HYT25815.1"
                     /translation="MRRLIGIAAVGLAFVLVAAGCGKKSNNQPVTAGNQGGNAKSLSG
                     AGATFPTPVYEKWFADFARTDPGKGVQVNYQSIGSGGGIKQFTAGTVDFGATDSPMKD
                     DEVQAAEQKGGTVIHVPTVLGSIVVSYNLPGVQQPLKMDGQLIGDIFLGKVKSWNAPE
                     IAKLNAGVTLPNLPILTVHRSDSSGTTGNFTKFVADTNKEFDSRIGAGKEVKWVGGVA
                     GKGNDGVTANVQQTKGAVGYVELNYALKNNLEFAEVKNAAGKFVTPSVESTTTAASDL
                     SSVPDDFRKSLVNSKSPDAYPIATWTFLVVFQKQQDATKGRTLASLLWYVTHGAQTSA
                     ASLDYAPLPQGVIPKIEAKIKSITGPDGKVLYAGQ"
     gene            1698..2327
                     /locus_tag="VEP73_04960"
     CDS             1698..2327
                     /locus_tag="VEP73_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYT25816.1"
                     /translation="MKVRIFPREESYFDLFEAAADNVAASARQLSDLVNDFVDPEMKA
                     KRLVELEHEGDRITHAILSRLNSTFITPFDREDIYALASQLDDVTDAIEEAADMLVLH
                     KVVEPIQAVRGQAALIQEAAIVTADGLRHLRKVEHEPLRAYWVRINELENDGDRLYRR
                     ARADLYAFTGEHPARYVLIWKDIVEQLEEALDGLEDVAHTVESIVLKHA"
     gene            2320..3297
                     /locus_tag="VEP73_04965"
                     /pseudo
     CDS             2320..3297
                     /locus_tag="VEP73_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            3482..5068
                     /locus_tag="VEP73_04970"
     CDS             3482..5068
                     /locus_tag="VEP73_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HYT25817.1"
                     /translation="MEPVRLAVLDVGSNTVHLAVVDGQPDGTFVPVARERETLRLAEA
                     AFPAMLLPDEAVERLTATVARMRARADELRADALVGFATSAIREARNGLEALGRVREA
                     TGVGVAVLPGVEEARLTYLATRRWTAFSARRLLVLDIGGGSLEIAAGEGDRPELAESL
                     PLGATRLSRRFVRSDPVHPDELVALRVHALALLGPLADRIRAQEHEVVCATSKTFRNL
                     GEIARALPEAPTPPHAFGFAGVDGQTAPVLTREALDVVAGYLAGTTARQRSRLAGLDA
                     LRAGNAVAGSQVAALVMQAFGLRQLVLAPWALREGVILDHLARHHDPGRPAGPSDDPR
                     RRRAVLDFARRHAWDEPHCRQVTALALSLFDQTLALHGLGPAERGLLEVAGLLHDVGY
                     AVAQSAHHKHSLYLIRNADLDGFSPRERDLVANVARYHRKALPSDRHAEYMALDDRDR
                     GVARKLAALLRVADGLDADHFQVVEGVTVADADADGLVRLELRARDVPDLDMWAAERN
                     GDLFELEFGRRVEPVAVEVA"
     gene            complement(5112..5282)
                     /locus_tag="VEP73_04975"
     CDS             complement(5112..5282)
                     /locus_tag="VEP73_04975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HYT25818.1"
                     /translation="MGQLYRPAYFYGGYAIHGSYSVPSYPASHGCVRVTIANMDRMYP
                     TLRIGTPVKVYR"
BASE COUNT          659 a         1927 c         1870 g          830 t
ORIGIN      
        1 tcgggtcccg gggcggcacg ctggagcggt tcgctcggcg gggcgccgcc cgggcgttcg
       61 gcgcgcgccg gccgcgggcg gtgctggtca gccatggggc tcctcctgga cgagcgggcg
      121 cggggttgtc aggtggggac cggccgggtg ggatgcctct cggcccggcg cggagggcgc
      181 cgggccgagg gtacggcaac cgaggtcgct gtgacctcgg gcggaccggt ccctactggc
      241 cggcgtagag caccttgccg tccgggcccg tgatcgactt gatcttcgcc tcgatcttcg
      301 ggatcacgcc ctgcggcagc ggggcgtagt cgaggctcgc ggcggaggtc tgggcgccgt
      361 gggtcacgta ccacaggagg ctggcaaggg tcctgccctt ggtggcgtcc tgctgcttct
      421 ggaacacgac caggaacgtc caggtcgcga tcgggtaggc gtccggcgac ttggagttga
      481 ccaggctctt gcggaagtcg tcgggcacgc tcgagaggtc gctggcggcc gtggtcgtgg
      541 actcgacgct cggcgtgacg aacttgccgg cagcgttctt cacctcggcg aactcgaggt
      601 tgttcttgag cgcgtagttg agctcgacgt agccgaccgc gcccttcgtc tgctggacgt
      661 tggcggtcac gccgtcgttg cccttgccgg ccacgccgcc gacccacttg acttccttgc
      721 ccgccccgat cctggagtcg aactccttgt tcgtgtcggc gacgaacttg gtgaagttgc
      781 cggtggtgcc cgaggagtcc gaacggtgca cggtcaggat cggcaggttg gggagggtca
      841 cgccggcgtt cagcttcgcg atctcagggg cgttccagct cttcaccttg ccgaggaaga
      901 tgtcgccgat gagctggccg tccatcttca gcggctgctg cacgccgggc aggttgtagc
      961 tgacgacgat cgagccgagc accgtcggga cgtggatgac ggtcccgccc ttctgctcgg
     1021 cggcctgcac ctcgtcgtcc ttcatcggcg agtccgtggc gccgaagtcg accgtcccgg
     1081 cggtgaactg cttgatgccg ccgccggagc cgatggactg gtagttgacc tggacgccct
     1141 tgcccgggtc ggtccgggcg aagtcggcga accacttctc gtagaccggc gtcgggaacg
     1201 tcgcgcccgc cccgctcagc gacttcgcgt tgccgccctg gttgccggcg gtcaccggct
     1261 ggttgttgga cttcttgcca caaccggcgg cgaccagcac gaaggcgagg ccgacagcgg
     1321 cgatgccgat gagccggcgc atcggtggct tgcgaacctg gtgcaactgg tcctccttgg
     1381 tcatcggcct tggtcatcgg ccttggtcat cggcctcggt catcggcctc gggtgatggg
     1441 cctcggtcat cgacggcagc ggtggagccg gtggcccgct cgaccgctcc ctgactccct
     1501 acgttgtagc gatgccgcgt ggcaccgccc ggcacgccag atggcgggat ggtggcgcgg
     1561 aggtgaacac tcgcgtcccg cacgggtcag gacaccgcga cgtcgcctcc cgttcaccgg
     1621 atcgacgtct ggtggacgcg cctgctaccg ttcgaatgcg gcgtgggcgg cgcctcatgg
     1681 gcttcattgg aggacgtgtg aaggtgcgga tctttccccg cgaggagtcc tacttcgacc
     1741 tgttcgaggc ggcggccgac aacgtggccg cgtccgcccg gcagctctcc gacctcgtca
     1801 acgacttcgt cgacccggag atgaaggcca agcgcctcgt cgagctcgag cacgagggcg
     1861 accgcatcac ccacgcgatc ctctcccgcc tcaactcgac gttcatcacc cccttcgacc
     1921 gcgaggacat ctatgcgctg gcctcgcagc tcgacgacgt caccgacgcg atcgaggagg
     1981 ccgccgacat gctcgtgctc cacaaggtgg tcgagcccat ccaggcggtc cgcggccagg
     2041 ccgcgctcat ccaggaggcg gccatcgtga ccgcggacgg gctccgccac ctccgcaagg
     2101 tcgagcacga gccgctgcgc gcctactggg tccgcatcaa cgagctggag aacgacggcg
     2161 accgcctgta ccggcgcgcc agagccgacc tgtacgcgtt caccggcgag cacccggcgc
     2221 ggtacgtgct catctggaag gacatcgtcg agcagctcga ggaggcgctc gacggcctcg
     2281 aggacgtcgc ccacaccgtc gagagcatcg tgctcaagca tgcctgacgc gcacctgctc
     2341 gtcgtggtgg ccatcgtcgc cctggcgctc ctcttcgact tcaccaacgg cttccacgac
     2401 gcggcgaact ccatcgccac cgtggtgtcc acccgcgtgc tcacgcccag ggcggccgtc
     2461 gtgtgggccg gcgtgttcaa cttcgtcgcg ttcctcgtct tcaagacgaa ggtcgccaac
     2521 acggtgggca agaccgtcga cccggcgatc gtcagcgagg cggtcatctt cgccggcctg
     2581 ctcggcgcca tcgcctggaa catcaccacc tggtggttcg ggctgccgac ttcgtcgtcg
     2641 cacgccctga tcggcgggtt cgcgggcgcc ggcatcgcca aggccgggtg gtcggtgatc
     2701 aactcgtcca gcatgcagaa gacggtcctg ttcatcttca tctcgccgct gttcggcttc
     2761 gcgctcgcct tcgccctgat gctcgcggcg ctctggatct tccaccggtc ggcgccgaga
     2821 cgggtggaca ggctgttccg gcgcctccag ctcttgtcgg ccgcggcgtt cagcctcggg
     2881 cacggcggca acgacgcgca gaagacgatg ggcatcatcg cggcgctgct ggtcggctcc
     2941 ggctacctcc acctgcagcc gaacggcaac ctgccggtgc cgctctggat cgtgctcgcc
     3001 gccaacgccg cgatcgcgct cggcaccctc tcgggcggct ggcggatcgt gcggaccctg
     3061 ggcaccaaga tcacggccct caagccgatc gccgggttcg cggccgagac ggcggccgcc
     3121 gtctccatct acgtggccac cttcctcggc atcccggtct cgaccacgca cacgatcacc
     3181 ggcgcgatcg tcggggccgg ctcgacgcgg gggctctcgg ccgtgcgctg gggcgtcgcc
     3241 ggccgtatcg tatgggcctg ggtcatcacg atcccggcgg cggcgctcgt cgcggcggtc
     3301 gcctaccggg tcgtcaccct gccgaaccac gtcctcgcgt tcctgctgct cgccctggtg
     3361 gcgctcgtgg tggccgtcct ggccggccgt cggctgtcgc gccggccggt cgtcgaggcg
     3421 cggacctagc cctcccgggg gtccgggggg ctcgagccca gccacccgga gagaggggga
     3481 ggtggagccg gtgcggctcg ccgtgctcga cgtggggtcg aacaccgtcc atctggcggt
     3541 ggtggacggc cagcccgacg gcaccttcgt cccggtcgcg cgggagcgcg agaccctgcg
     3601 cctcgccgag gccgccttcc ccgccatgct gctgcccgac gaggccgtcg aacggctcac
     3661 cgccaccgtc gcccgcatgc gcgccagagc cgacgagctg cgggccgacg cgctggtcgg
     3721 cttcgcgacc tcggcgatcc gggaggcgcg gaacgggctc gaggcgctcg gccgcgtccg
     3781 cgaggcgacc ggcgtcgggg tggccgtgct gcccggggtc gaggaggcgc gcctcacgta
     3841 cctggccacc cgccgctgga ccgcgttctc ggcccgccgt ctcctcgtgc tcgacatcgg
     3901 cggcggcagc ctcgagatcg ccgcggggga gggcgaccgg ccggagctgg ccgagagcct
     3961 tccgctcggg gcgacccggc tctcccgccg gttcgtccgc tccgaccccg tgcaccccga
     4021 cgagctggtc gcgctgcgcg tccacgcgct cgcgctgctc ggcccgctcg ccgaccgcat
     4081 ccgcgcccag gagcacgagg tcgtgtgcgc cacctcgaag acgttccgca acctgggcga
     4141 gatcgcgcgg gcgcttcctg aggcgccgac cccaccccac gccttcggct tcgccggggt
     4201 cgacgggcag accgcgccgg tgctcacccg cgaggcgctc gacgtggtgg ccggctacct
     4261 ggccggtacg acggcccgcc agcgcagccg cctcgccggg ctggacgccc tgcgtgccgg
     4321 caacgcggtc gccggctccc aggtggcggc gctcgtcatg caggcgttcg ggctgcgcca
     4381 gctggtgctc gcgccctggg cgctgcgcga gggggtcatc ctcgaccacc tggcccgcca
     4441 ccacgacccg ggccggcccg ccgggcccag cgacgacccg aggcgccgcc gcgcggtgct
     4501 cgacttcgcc cgccgccacg catgggacga gccgcactgc cgccaggtca ccgccctcgc
     4561 gctgtcgctg ttcgaccaga ccctggcgct gcacgggctc ggccccgccg agcgcgggct
     4621 gctcgaggtc gcgggcctcc tccacgacgt ggggtacgcg gtcgcccagt ccgcccacca
     4681 caagcactcg ctctacctga tccgcaacgc cgacctggac ggcttctcgc cccgcgagcg
     4741 cgacctcgtc gcgaacgtcg cccgctacca ccgcaaggcg ctgccgtccg accgccacgc
     4801 cgagtacatg gccctcgacg accgcgaccg cggggtggcg cgcaagctcg ccgccctcct
     4861 gcgcgtggcc gacgggctgg acgccgacca cttccaggtc gtcgagggcg tcaccgtcgc
     4921 cgacgccgac gccgacggcc tggtccgcct cgagctgcgt gcccgcgacg tgcccgacct
     4981 cgacatgtgg gcggcggagc gcaacggcga cctcttcgag ctggagttcg gccgccgcgt
     5041 cgagcccgtg gccgtcgagg tggcctagcc ggtcgccgtc gaggtcgcgc agcgaggggc
     5101 gtcccggtcg gtcagcggta gaccttgacc ggggtgccga tcctgagggt cgggtacatc
     5161 cggtccatgt tggcgatcgt gacgcggacg cagccgtggc tggccgggta ggacgggacc
     5221 gagtacgagc cgtggatcgc atagccgccg tagaagtagg ccgggcggta gagctgcccc
     5281 agcggg
//
LOCUS       DASPYY010000324         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_336635, whole genome shotgun sequence.
ACCESSION   DASPYY010000324 DASPYY010000000
VERSION     DASPYY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336635"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..955)
                     /locus_tag="VEP73_04980"
     CDS             complement(92..955)
                     /locus_tag="VEP73_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYT25819.1"
                     /translation="MPDHPDREDLAAWQAGALDEPDRDRTASHVAGCPDCGRVVGALG
                     RVRQRLALLDEPELPAGFHDRLAAAVDAERAWQVPRQRPAWYRRPAAWGAAAAVLLFV
                     AGIFSLIRLSNESGSTGSGAATAAPLGNQGDGGYGNGAAGMVPGAPPAPLLVVRVPGE
                     FSPTRLRNAVRASPTALRALRLAERAAPVQPGTVQRAESQPDTRMSGSGASRQPSLAP
                     ADQACVDRVAPPGGVRPALIADTRYQGRPARVLVAYVGDSTRLARFWVFPDGGCGRAP
                     LTSGTADLGTG"
     gene            complement(963..1655)
                     /locus_tag="VEP73_04985"
     CDS             complement(963..1655)
                     /locus_tag="VEP73_04985"
                     /inference="COORDINATES: protein motif:HMM:NF007225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT25820.1"
                     /translation="MSDLTDEELLAAHVRGDRFAFGQLVARHERRVFGICLRMLGNRE
                     DAQDAAQDAFLAALRRASSFRQAAAFSTWLYRIAVNAATDQARRRVRARTTPLGPEDA
                     GLPGDAATADPGDTVATAVAVQSALTRIPEDFRAAVVLCDLVRLPYSEAADILEVPVG
                     TLKSRVFRGRLALAEQLSELGRPEPWQAVGKRPVELRVVGAPGAPAPAAGTGARARAS
                     EPVDVEPHAHEE"
     gene            complement(1726..3243)
                     /locus_tag="VEP73_04990"
     CDS             complement(1726..3243)
                     /locus_tag="VEP73_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT25821.1"
                     /translation="MSMSQVGSGLPRLLAERYELSERLASGGMTSVWRGHDRVLGRDV
                     AIKVLHPQLAADPSFRARFSEEAVNAARLTHPNIVALYDTGEQNGVAYIVIEWVEGAS
                     LAELLARHGPLPPAKAARLASEVALALDYAHGAGVCHRNLKPANILISEDGTVKVADF
                     SIAKAATEDDPARTGELLGATGHLAPEQAEGDEVDGRADVYGLGACLFEMLTGRSPSA
                     AWRGSSGTMSPRSVRAGVPRELDAVVMRAMAHDPADRYPTAGSMASALARPAAADDGG
                     TRAIVPEPPEPPGPTDSEPEASFIRHEGRWLGWTLVLVGLAAVLVVVGLTLSKTVGPI
                     LGSSGKPKSRSTTTAGTSPASTQLRVAVAHSFDPYGDKTENEATAPEAIDSDKGSAWR
                     TEGYNDADLVPYKRGVGLVLDLGSARRVRSIALTLLAPGAVVTVYGAEGQDGGYPPDL
                     ESGWTALTDTVQRDQSSIVPLRGGPYRYYLIWFTRLPPDGKGKFRDGIADAVLRS"
     gene            complement(3379..>3710)
                     /locus_tag="VEP73_04995"
     CDS             complement(3379..>3710)
                     /locus_tag="VEP73_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYT25822.1"
                     /translation="LGKPYVWGATGPGGFDCSGLTMAAYAHAGLSLPRTSRSQWYAGP
                     HVELVDLAPGDLLFFADDTSSPSSIHHVGMYVGGGRMVEAPYTGADVRVSSIGRPDYI
                     GAVRPTG"
BASE COUNT          458 a         1364 c         1402 g          486 t
ORIGIN      
        1 ggcggggtcg tggcctattt cagggctggc gggacctggt agccggccgc caggccggcg
       61 tggcccggtg ccggcccggc ccggtggggg atcagcccgt gccgaggtcg gccgtgcccg
      121 acgtgagcgg ggccctcccg cagccgccgt ccgggaacac ccagaagcgc gccagccggg
      181 tcgagtcgcc gacgtaggcc acgagcaccc tcgcgggccg gccctggtac ctcgtgtcgg
      241 cgatgagcgc cgggcggacg cccccgggcg gggcgacccg gtcgacgcag gcctggtccg
      301 ccggtgccag cgagggctgg cgcgaggccc cgctgccgga catccgggtg tcgggctggc
      361 tctcggcgcg ctggaccgtc ccgggctgga cgggcgcggc ccgctcggcg agccgcagcg
      421 cccgcagcgc ggtcgggctg gcgcgcaccg cgttcctgag ccgggtcggg gagaactcgc
      481 cgggcacccg gacgacgagc aacggggcag gaggcgcccc cggcaccatc cctgctgcac
      541 cgttgccgta ccctccgtcg ccctggttgc ccagcggcgc cgcggtcgcg gcgccgctgc
      601 ccgtcgaccc ggattcgttg ctcaggcgga tcaggctgaa gatcccggcc acgaacagga
      661 gcaccgcggc ggcggcgccc caggcggccg gccgccggta ccaggccggc cgttggcgtg
      721 ggacctgcca tgcccgctcg gcgtcgaccg ccgccgccag acggtcgtgg aacccggccg
      781 ggagctccgg ctcgtcgagc agcgcgagcc gctggcgcac cctgccgagc gctcccacca
      841 ccctcccgca gtccgggcag ccggccacgt gggacgcggt tcggtcccgg tcgggctcgt
      901 cgagggcgcc cgcctgccag gcggcgaggt cctcacggtc ggggtggtct ggcacggtcc
      961 tgctactcct cgtgggcatg tggctcgaca tccaccggtt cggacgctcg cgcccgcgcc
     1021 ccggttcccg ccgccggtgc cggcgcgccc ggtgcgccga cgacccggag ctcgaccggc
     1081 cgcttcccga ccgcttgcca gggctcgggc cggccgagct cgctcaactg ctcggccagc
     1141 gcgagccggc cccggaacac gcggctcttg agcgtcccga ccggcacctc gaggatatcg
     1201 gcagcctccg aatacggcag ccggacgagg tcgcagagga ccacggcagc ccggaagtcc
     1261 tccgggatgc gggtcagggc ggactgcacg gcgacggcgg tggcgacggt gtcgcccggg
     1321 tcggccgttg ccgcgtcacc gggcagcccg gcgtcctccg ggccgagcgg cgtggtgcgg
     1381 gcccgcaccc gccgcctcgc ctggtcggtg gccgcgttga cggcgatccg gtagagccag
     1441 gtcgagaatg ccgccgcctg gcggaagctc gaggcacggc gcagcgcggc caggaaggcg
     1501 tcctgcgcgg cgtcctgcgc gtcctcccgg ttgccgagca tccgcaggca gatgccgaac
     1561 acgcgccgct cgtgcctggc cacgagctgg ccgaaggcga accggtcccc ccgcacgtgc
     1621 gcggccagca gctcctcgtc ggtcaggtcg ctcatctgac cacccgccgc gggcgctcat
     1681 tccgatcacc ccgccgcggg tgccgggcca ccccgtcgcg ggcgctcacg acctgaggac
     1741 cgcgtccgcg atcccgtccc ggaacttccc cttgccgtcc gggggcagcc tggtgaacca
     1801 gatcaggtag taccggtagg ggccgcccct gagcgggacg atgctcgact ggtcccgctg
     1861 caccgtgtcc gtgagcgccg tccagccgct ctccaggtcg ggcgggtagc cgccgtcctg
     1921 gccctccgcg ccgtacacgg tcacgacggc acccggggcc agcagggtca gggcgatcga
     1981 ccggacccgc ctggcgctgc cgaggtcgag caccaggccc acaccgcgct tgtacggcac
     2041 caggtccgcg tcgttgtacc cctcggtcct ccaggcgctc cccttatccg agtcgatagc
     2101 ttcgggggct gtggcctcgt tctctgtctt gtcgccatag ggatcgaacg agtgcgccac
     2161 ggcgacccgc agctgcgtgc tggccggcga ggtgcccgcg gtcgtcgtgg acctggactt
     2221 cggcttgccg ctcgagccga ggatcggccc gacggtcttg gagagggtga gaccgaccac
     2281 caccaggacg gcggcgagcc cgaccagcac cagggtccag ccgagccagc ggccctcgtg
     2341 gcggatgaag ctcgcctccg gctcggagtc ggtggggccg ggcggctcgg gcggctcggg
     2401 cacgatcgcc ctggtgccgc cgtcgtccgc agccgccggc ctggcgagcg ccgacgccat
     2461 gctcccggcg gtcgggtagc ggtcggcggg gtcgtgggcc atcgcccgca tgaccacggc
     2521 gtcgagctca cggggcaccc ctgcccgcac cgagcgtggg gacatggtgc ccgagctccc
     2581 gcgccaggcc gcggagggcg accgtccggt gagcatctcg aacaggcagg cgccgaggcc
     2641 gtagacgtcc gcgcgcccgt ccacctcgtc cccctcggcc tgctcggggg ccaggtggcc
     2701 ggtggcgccg agaagctcgc cggtgcgggc cgggtcgtcc tcggtggccg ccttggcgat
     2761 cgagaagtcg gccaccttca cggtgccgtc ctcgctgatc aggatgttgg ccggcttgag
     2821 gttgcggtgg cagaccccgg cgccgtgggc gtagtcgagg gcgagggcga cctcgctcgc
     2881 gaggcgggcc gccttggcgg gcggcagcgg gccgtgacgg gcgagaagct ctgccaggct
     2941 cgccccctcg acccactcga tgacgatgta ggcgaccccg ttctgctccc ccgtgtcgta
     3001 gagggcgacg atgttcgggt gggtgaggcg tgccgcgttc acggcctcct cggagaagcg
     3061 cgcacggaag ctggggtcgg cggccagctg agggtgcagc actttgatgg ccacgtcgcg
     3121 tccgaggaca cgatcgtgac cgcgccagac gctggtcatg ccaccagatg ccagtcgttc
     3181 gctgagctcg tagcgctcag cgaggagtcg gggcagaccg gaaccgacct gcgacatgct
     3241 cataagcaaa gtagcggggc gttgggcccc ggaaatcgcg acaggggcag tctagtgcac
     3301 caggatgctt ccgttcatcc ggttgggcat ggtcgcccgg gagcgtcgcc ccggagcgcg
     3361 tccaggcccg cggtcgcgtc acccggtcgg gcggacggcg ccgatgtagt ccgggcggcc
     3421 gatcgagctg acgcggacgt ccgcgccggt gtagggcgcc tcgaccatgc gaccgccgcc
     3481 gacgtacatg cccacgtggt ggatcgagct cgggctggag gtgtcgtcgg cgaagaacag
     3541 caggtcgccc ggtgccaggt cgacgagctc gacgtgcggg cccgcgtacc actgcgaccg
     3601 gctcgtgcgg gggagcgaga gcccggcatg ggcgtacgca gccatcgtga ggcccgagca
     3661 gtcgaagccg cccggaccgg tggcgcccca gacgtacggc ttgccgagct
//
LOCUS       DASPYY010000325         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2584138, whole genome shotgun sequence.
ACCESSION   DASPYY010000325 DASPYY010000000
VERSION     DASPYY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584138"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEP73_05000"
     CDS             <1..295
                     /locus_tag="VEP73_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT25823.1"
                     /translation="IDHPSVEREVEIVRVRAPEVPEMLARQVAHAVARLRSMDVAKRP
                     GVAETIDWSVALAFLGATGLERETAAATLGALVKDHEDLELVRSRLDQILSAE"
     gene            292..1470
                     /locus_tag="VEP73_05005"
     CDS             292..1470
                     /locus_tag="VEP73_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT25824.1"
                     /translation="MTAAALEVRGLERLVGFGRLLRARGLPVGTGRILSFCRAVAALG
                     SVDRDALYWAGRVTMVARREDLDAYDRAFRAWFRPPDGDARTATGDRGGVGQPPGIDI
                     EVVVEEGIAVSGSLEEAGERDGEVLVGVLASGAELLRGKSFEELSEQERHAADQLIRE
                     LTVRLPERRARRSRPSPYGRQFDLRRTVRASLRTQGEPFRRAFRKRRWKRRPLVLILD
                     VSGSMSAYSRALMQFGFAAIRAGRRVEVFCFGTRLTRVTRALRRSEPDTAMREVAAEV
                     VDWDGGTRIGASLKELLDRYGQHAFLRGAVVVLCSDGLERGDPALLAAQMARLGRLAS
                     RLVWVNPLKGSPRYEPLARGMAAALPHVDRFLPGHNLSSLEALCEVLAGHAAGQGHGR
                     "
BASE COUNT          169 a          600 c          608 g          215 t
ORIGIN      
        1 gatcgaccac ccgtcggtcg agcgcgaggt cgagatcgtc cgcgtccgcg cccccgaggt
       61 ccccgagatg ctcgccaggc aggtcgccca cgccgtcgcc cggctgcggt cgatggacgt
      121 cgccaagcgg cccggggtcg ccgagacgat cgactggtcg gtggcgctcg ccttcctcgg
      181 cgcgaccggg ctcgagcgcg agaccgccgc ggcgaccctc ggcgccctgg tcaaggacca
      241 cgaggacctc gagctcgtcc gctcccgcct cgaccagatc ctgagcgcgg aatgacggcg
      301 gcggccctgg aggtccgggg gctcgagcgg ctggtcgggt tcgggcgcct gctgcgggcc
      361 agagggctgc cggtcggcac cggccggatc ctctccttct gccgggcggt cgccgccctc
      421 ggctcggtgg accgcgacgc gctgtactgg gccggccggg tcaccatggt cgcccgccgg
      481 gaggacctgg acgcctacga ccgcgccttc cgcgcctggt tccgtccgcc cgacggggac
      541 gcccggacgg ccaccggcga ccgcggcggg gtgggccagc cgccaggcat cgacatcgag
      601 gtcgtcgtcg aggaggggat cgccgtgtcc ggctcgctcg aggaggccgg cgagcgcgac
      661 ggggaggtgc tcgtcggcgt gctcgcgagc ggggccgagc tgctgcgcgg gaagtcgttc
      721 gaggagctct ccgagcagga gcggcatgcg gccgaccagc tcatccgcga gctcaccgtg
      781 cggctgcccg agcggcgggc ccgccggtcg cggccctctc cctacgggcg ccagttcgac
      841 ctgcgccgga cggtgcgggc gtcgctgcgc acgcagggcg agccgttccg gcgcgcgttc
      901 cggaagcggc gctggaagcg ccgcccgctc gtgctgatcc tcgacgtgtc cgggtcgatg
      961 tcggcctact cgcgcgccct gatgcagttc ggcttcgcgg ccatccgggc cggccggcgc
     1021 gtggaggtgt tctgcttcgg cacccggctg acaagggtca cgcgcgccct gcggcgctcc
     1081 gagcccgaca cggccatgcg cgaggtggcc gccgaggtgg tcgactggga cggcggcacc
     1141 cgcatcgggg cgtccctcaa ggagctgctc gaccgctacg ggcagcacgc gttcctccgc
     1201 ggggcggtcg tcgtgctctg ctcggacggg ctcgagcggg gcgaccccgc gctgctcgcc
     1261 gcccagatgg cccggctcgg ccgcctcgcc tcccggctcg tctgggtcaa cccgctgaag
     1321 ggcagccccc gctacgagcc cctggccagg ggcatggccg cggcgctgcc gcacgtcgac
     1381 cgcttcctgc ccggccacaa cctgtcgagc ctcgaggcgc tgtgcgaggt gctcgccggc
     1441 catgccgccg gccaggggca cggacgatga cggtcccagg ggggccggtg tacggtgccg
     1501 gcgcgatgga cgtgcaaccg gtggtgaccg tgctgctgac cggcgcgccg ctctccctcg
     1561 acgccgcctt ccggtccgtg ctcctgcccg gc
//
LOCUS       DASPYY010000326         5168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2444365, whole genome shotgun sequence.
ACCESSION   DASPYY010000326 DASPYY010000000
VERSION     DASPYY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444365"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="gcvT"
                     /locus_tag="VEP73_05010"
     CDS             <1..1047
                     /gene="gcvT"
                     /locus_tag="VEP73_05010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYT25825.1"
                     /translation="GWDMPIEYAGTLAEHRAVREAAGVFDLSHLGKVEVRGAGAHDTL
                     QHALSNDLDRLATEGAAQYTLCLADEGGIVDDLIAYRERDGYLLVPNAANWPAVAELV
                     RASAAGLVRGGAPGTEVTPRPDLAVLAVQGPRAPELVGSLFPEAKGLAYMHLAPVAFA
                     GEPGRLCRTGYTGERGYELIVPGQVAGKLWDEVLERGAPLGAVPAGLGARDTLRLEMG
                     YPLHGNDISTATDPISARLGWAVAMAKDGFRGRDALVARKSAGPERVLVGLAATDRLI
                     PRHGMAVLDEGDRPVGEVTSGTFSPTLRHGIALAYVVPEVAGEGTGLQVDVRGRRRGA
                     VRVTRPPFVDASPR"
     gene            complement(1059..3728)
                     /gene="aceE"
                     /locus_tag="VEP73_05015"
     CDS             complement(1059..3728)
                     /gene="aceE"
                     /locus_tag="VEP73_05015"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYT25826.1"
                     /translation="MLDQLPDPDPEETGEWLEALAGVVEHEGPARARFLLRKVLERAG
                     ALEVGLPEPPSTDYVNTIPAGAEPEYPGDEELERRLLAIVRWNAAAVVSRANRPELGV
                     GGHMASYASAAELYETGFNHFFKGKEGGHGDQVFFQGHSSPGVYARAYLEGRLSDEQL
                     DAFRQEGKPGGLSSYPHPRLMPDFWEFPTVSMGLGPLNAIYQARFNKYLAHQGRADTS
                     GSRVWAFVGDGEMDEPEAQGALTVAARDQLDNLTFVLNCNLQRLDGPVRGNGKIIQEM
                     ESLFRGAGWNVIKVVWGRAWDDLLARDSTGALVAKLNSLPDGEFQTLAAEDATYIREH
                     LFDSDELRRLVEGMSDEEIVHLSLDRGGHDLRKVYAAFKAAVETRGRPSVVIAHTIKG
                     RGLGREFEARNATHQMKKFVRGTLKTFRDRLGIDVSDEQIEKGYPPYYRPPGDSDLHR
                     YLLERRERLGGPVPRRVVRAGPLNLPAKDLYAQLKKGSGKQEVATTMALVRLVKDLLR
                     NDDVGKRIVPIIPDEARTFGMDSLFPSLKIYSPRGMNYDAVDRDLVLSYKEDPKGQIM
                     HEGITEAGAMGTFHAAGSSYATFGEPMIPLYIFYSMFGFQRTGDEMWSAGDQRTRGFL
                     IGATAGRTTLNGEGLQHQDGHSLLIATTNPACVAYDPSFAFEIPVIVEDALRRMYGEH
                     PEDVFYYLTVYNEPFVQPPMPEGLDENLILQGLYRYRAAEGEHATRAHIATSGSAMPL
                     ALKAQELLASEFDVGADVWSAPGWQRLREEALACEEWNRMHPTEELRVPLVTRTFEDE
                     EGPIVAVTDWMKAVPDQISRWVPGPYAVLGTDGFGRSDTRPALRRHFRIDAESIAVAV
                     LYELALFGRVERDTVAEAIKRFDLDPEATTEVP"
     gene            complement(3805..4740)
                     /locus_tag="VEP73_05020"
     CDS             complement(3805..4740)
                     /locus_tag="VEP73_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYT25827.1"
                     /translation="MRTTSRPSATLGALLRVVAAAACWGLAAVFAKHAFEHGVPPARM
                     AEARVLVALAVLGPLLAWHRPALLRPPRSSLPVLLAFGVCIAAVNFTYYVAIDHLAVG
                     VAISLQYTGPVLVLGFSALVARRAPGGLAWVAAGLTLAGAVLVSQALDGLDVLDGTGL
                     LAGLTSAVLFAAYLLTAERAGRLGVPPETVLLWGFVAAVAVWSVAAPWWSWPLARLGD
                     PRVALAVLGVGVVGTLVPFFLAVGAVRVIPAATAAIAASSEPVFAAGLAWLLLGQQLA
                     AAQLAGGALVVAGVMLAQLATQREPDVVAAREGVD"
     gene            5002..>5168
                     /locus_tag="VEP73_05025"
     CDS             5002..>5168
                     /locus_tag="VEP73_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HYT25828.1"
                     /translation="MADSNKGVVYTGPGNVEVQDLDYPKLELPEQNRKLEHGAILKVV
                     ATNICGSDQHMV"
BASE COUNT          707 a         1852 c         1915 g          694 t
ORIGIN      
        1 ggctgggaca tgccgatcga gtacgccggc acgctcgccg agcaccgcgc cgtccgcgag
       61 gcggccggcg tcttcgacct gtcccacctc ggcaaggtcg aggtacgcgg cgcgggcgcc
      121 cacgacaccc tccagcacgc cctgtccaac gacctcgacc ggctcgcgac ggagggcgcc
      181 gcccagtaca ccctgtgcct ggccgacgag ggcggcatcg tcgacgacct catcgcctac
      241 cgggagcggg acggctacct gctcgtgccc aacgcggcca actggccggc agtggccgag
      301 ctggtgcgcg cctccgccgc gggcctggtg cgcggaggcg caccgggcac cgaggtcacc
      361 ccgcggcccg acctcgcggt gctcgccgtc cagggtccgc gcgcccccga gctcgtcggg
      421 agcctgttcc ccgaggccaa gggcctcgcc tacatgcacc tcgccccggt cgccttcgcc
      481 ggcgagccgg gccgcctgtg ccgcaccggc tacaccgggg agcgcgggta cgagctgatc
      541 gtgcccgggc aggtggccgg caagctctgg gacgaggtgc tcgagcgcgg cgccccgctc
      601 ggcgcggtcc cggccggcct gggcgcgcgc gacacgctcc gcctcgagat gggctatccc
      661 ctccatggca acgacatctc gaccgccacg gatccgatct cggcccgcct cggctgggct
      721 gtggccatgg ccaaggacgg cttccggggc cgcgacgccc tcgtcgcccg caagtcggcc
      781 gggccggagc gggtgctcgt cggcctcgcc gccaccgacc ggctgatccc gaggcacggc
      841 atggccgtgc tcgacgaggg cgaccgcccg gtgggggagg tcacgagcgg cacgttctcg
      901 cctaccctgc ggcacgggat cgcgctggcc tacgtcgtgc ccgaggtggc aggcgagggc
      961 accgggctgc aggtcgacgt gcgcggccgc cggcgggggg ccgtccgcgt gacccgcccg
     1021 ccgttcgtcg acgcctcgcc caggtaggct ggcgcccgtc aggggacctc ggtcgtggcc
     1081 tcggggtcga gatcgaagcg cttgatggcc tcggcgaccg tgtcgcgctc cacccgcccg
     1141 aagagggcga gctcgtagag gaccgcgacc gcgatgctct cggcgtcgat ccggaagtgc
     1201 cgccgcaggg ccgggcgggt gtcggagcgg ccgaacccgt cggtcccgag gacggcgtac
     1261 ggcccgggga cccagcggga gatctggtcg gggacggcct tcatccagtc ggtgaccgcg
     1321 acgatcggtc cctcctcgtc ctcgaaggtg cgggtcacca gcgggacccg cagctcctcg
     1381 gtcgggtgca tgcggttcca ctcctcgcag gcgagcgcct cctcgcgcag ccgctgccag
     1441 cccggcgcgc tccacacgtc ggcgccgacg tcgaactcgc tcgcgagcag ctcctgggcc
     1501 ttgagggcca gcggcatggc gctgccgctg gtggcgatgt gcgcgcgggt ggcgtgctcg
     1561 ccctcggcgg ccctgtagcg gtacaggccc tgcaggatca ggttctcgtc gaggccctcg
     1621 ggcatgggcg gctgcacgaa cggctcgttg tagaccgtca ggtagtagaa gacgtcctcg
     1681 gggtgctcgc cgtacatgcg gcgcagcgcg tcctcgacga tcaccgggat ctcgaacgcg
     1741 aacgacgggt cgtaggcgac gcaggccggg ttcgtcgtcg cgatcagcag ggagtggccg
     1801 tcctggtgct ggaggccctc gccgttcagg gtggtgcggc cggcggtggc cccgatcagg
     1861 aagccgcggg tgcgctggtc gcccgcgctc cacatctcgt cgccggtccg ctggaacccg
     1921 aacatcgagt agaagatgta cagcgggatc atcggctcgc cgaaggtcgc gtagctcgac
     1981 ccggccgcgt ggaaggtgcc catcgcgccc gcctcggtga tcccctcgtg catgatctgg
     2041 cccttggggt cctccttgta ggacagcacc aggtcccggt cgaccgcgtc gtagttcatc
     2101 ccccgggggg agtagatctt gaggctcggg aacagcgagt ccatgccgaa ggtgcgcgcc
     2161 tcgtcgggga tgatgggcac gatgcgcttg ccgacgtcgt cgttgcggag caggtccttg
     2221 accaggcgca cgagcgccat ggtggtggcg acctcctgct tgccggagcc cttcttgagc
     2281 tgggcgtaga ggtccttggc cggcaggttg agcggcccgg cgcgcaccac ccggcggggc
     2341 acggggccgc cgagccgctc gcgccgctcg agcaggtagc ggtgcaggtc ggagtccccg
     2401 gggggccggt agtagggtgg gtagcccttc tcgatctgct cgtcggagac gtcgatgccg
     2461 agccggtcgc ggaacgtctt gagcgtgccc cgcacgaact tcttcatctg gtgggtggcg
     2521 ttgcgcgcct cgaactcgcg cccgagcccg cgtcccttga tcgtgtgggc gatcacgacg
     2581 ctcggccgcc ccctggtctc gaccgcggcc ttgaacgcgg cgtacacctt gcgcaggtcg
     2641 tggccgcccc ggtccagcga gaggtggacg atctcctcgt cgctcatgcc ctcgacgagc
     2701 cggcgcagct cgtcggagtc gaacaggtgc tcgcggatgt aggtggcgtc ctcggcggcg
     2761 agggtctgga actcgccgtc gggcagcgag ttcagcttgg cgaccagcgc cccggtgctg
     2821 tcccgcgcga gcaggtcgtc ccaggcgcgt ccccagacga ccttgatgac gttccagccg
     2881 gcgccgcgga acagcgactc catctcctgg atgatcttgc cgttgccgcg caccgggccg
     2941 tccagtcgct gcaggttgca gttgaggacg aaggtgaggt tgtcgagctg gtcgcgggcg
     3001 gccaccgtca gggcgccctg cgcttcgggc tcgtccatct cgccgtcgcc gacgaacgcc
     3061 cagacgcggg agccggaggt gtccgcgcgc ccctggtggg ccaggtactt gttgaagcgg
     3121 gcctggtaga tcgcgttgag ggggccgagc cccatcgaca cggtcgggaa ctcccagaag
     3181 tcgggcatga gccgcgggtg ggggtaggac gagagcccac ccggcttgcc ctcctgccgg
     3241 aaggcgtcga gctgctcgtc gctcaggcga ccctcgaggt acgcccgcgc gtagaccccc
     3301 ggcgaggagt ggccctggaa gaagacctgg tcgccgtggc cgccctcctt gcccttgaag
     3361 aagtggttga agcccgtctc gtacagctcg gcggccgacg cgtaggaggc catgtggccg
     3421 ccgacgccca gctcgggccg gttggcccgc gacacgaccg cggccgcgtt ccagcggacg
     3481 atcgcgagca gccggcgctc gagctcctcg tcgcccgggt actcgggctc ggcgccggca
     3541 gggatcgtgt tgacgtagtc ggtcgagggc ggctcgggca gcccgacctc gagcgcgccg
     3601 gcgcgctcga gcaccttgcg cagcaggaag cgggcccgtg ccgggccctc gtgctcgacc
     3661 acgccggcca gggcctcgag ccactcgccg gtctcctccg ggtccgggtc gggaagctgg
     3721 tcaagcatcc cgccacgggt gacgttcttg tcgtcgctca cggttcgctc ccggcctcgg
     3781 ctttctccgt ccaccttacc tacactagtc gaccccttca cgtgcggcga cgacgtctgg
     3841 ctcccgctgg gtggcgagct gggcgagcat caccccggcg acgaccaggg cgcccccggc
     3901 gagctgggcg gcggcgagct gctggcccag cagcagccag gccaggccgg ccgcgaacac
     3961 cggctccgag ctggccgcga tggccgcggt cgcggccggg atgacccgga cggcgccgac
     4021 cgcgaggaag aacggcacca gggtgccgac gacgccgacg ccgaggacgg ccagcgcgac
     4081 gcgggggtcg ccgagccggg cgagcggcca cgaccaccac ggggcggcaa cgctccacac
     4141 ggccacggcc gcgacgaagc cccacagcag caccgtctcc gggggcacgc cgagccgtcc
     4201 ggcccgctcg gcggtgagca ggtaggcggc gaacaggacc gccgacgtga gcccggcgag
     4261 caggccggtg ccgtccagca cgtcgagccc gtcgagggcc tggctcacca ggaccgcccc
     4321 ggccagggtg agcccggcag ccacccaggc cagcccgccg ggcgcccgcc tggccaccag
     4381 cgcgctgaac ccgagcacca ggacgggccc ggtgtactgc aaggagatcg ccaccccgac
     4441 cgccaggtgg tcgatggcga cgtagtaggt gaagttgacc gccgcgatgc agacgccgaa
     4501 cgcgagcagc acgggcaggc tggaccgcgg cgggcgcagc agggcaggcc ggtgccaggc
     4561 gagcagcggg ccgaggacgg cgagcgcgac caggacccgc gcctcggcca tccgggcggg
     4621 ggggacgcca tgctcgaacg cgtgcttggc gaacaccgcg gcaaggcccc agcacgcggc
     4681 ggcggccacc acgcgcagga gcgcgcccag cgtggcggag ggtcgggagg tggtgcgcat
     4741 cgcgtccagc ctagccgccc ggcgccagca tcgagactgg ttgggttgct tgacgacatt
     4801 cgacgagctt cgcgcccttg tccgtctttc ggggcggcgg tagggtggcc gctgcccgac
     4861 tcgcggaggt ggccgatgca gggaggcaag caggtcgcgc cggccccctg gcgcgcgcgc
     4921 gccccgccgt ggcagccgga ccgcgaggca cagcgtgcac ctggaccctc gtgcaccgcg
     4981 tcaccaacgg agggaacagc cgtggcggac agcaacaagg gtgtcgtcta caccggaccg
     5041 ggcaacgtcg aggtgcagga tctggactac cccaagctcg agctgcccga gcagaaccgg
     5101 aagctcgagc atggcgcgat cctcaaggtc gtcgcgacca acatctgcgg atccgaccag
     5161 cacatggt
//
LOCUS       DASPYY010000327         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_757934, whole genome shotgun sequence.
ACCESSION   DASPYY010000327 DASPYY010000000
VERSION     DASPYY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757934"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="ligD"
                     /locus_tag="VEP73_05030"
     CDS             <1..1017
                     /gene="ligD"
                     /locus_tag="VEP73_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYT25829.1"
                     /translation="ICFLAGKASLPQPAPPPTFEPMMAALETAPFDARGWLFEVKWDG
                     HRCLANLGTATRLTSRTGRDATAQFPELGEMHRQLAARNAVVDGEVVALDAEGRPSFA
                     RMQDRFHRSAEELARNAGRVPVQFIAFDLLWLDGVPLLDLPLSERRALLAEVFVETPR
                     MRLSQVVEEAGAAFFAEIEKLGLEGVVGKRLASPYRPGQRSPDWRKVKAVRRQDCVIV
                     GWTPGKGARAATLGALLLAVWDNGRLRYAGNVGTGFTQGFLTELLADLRERETGERPV
                     DPDPRVRGARWVRPELVCEVEHQGWTKEGRLRFASFKGIRMDKRPEDCLPEVAATGPA
                     GGRE"
     gene            1133..1828
                     /locus_tag="VEP73_05035"
     CDS             1133..1828
                     /locus_tag="VEP73_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25830.1"
                     /translation="MGAHILVVDDDPLLAAALRRPLAYEGFEVEVAASGEEALSRALE
                     RAPDLVILDVLLPGIDGLEVCRRLRQVGDAAVLMLTARSEVPERVAGFEAGADDYLGK
                     PFAFEELLVRVKALLRRGRNGGAPTAELRFGNVRMDRSTREVWRGDRTISLTRQEFDL
                     LALFLEHPRQVLPRSTILERVWDYDFGRDSNVLEVYVGYLRRKLETDDEPRVIHTVRG
                     VGYVLREDPEAAR"
     gene            1825..3396
                     /locus_tag="VEP73_05040"
     CDS             1825..3396
                     /locus_tag="VEP73_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25831.1"
                     /translation="MTGGGTTLMSFRARLTLAYLALLTLTLSAFGFGVYAYVDAKLHH
                     EFESAFLTQGRALGQLLQGYSSVNTNGVRSFMQQVRPSTYYVVTARRTPKDPYYPDVN
                     VRSQELTGTDVNLPGIPADRKVHTVPERQNELATALAVYSADFEAVEPPASPQGGGRA
                     QSDTTPARWQGRLVLARDLNSVEASLSLLRKILIAGGLAVLGLATLIGWVLAAGLLRP
                     LDRMKSTAQRIGDDRDFTRRMPVDNAYDELGRLSTSFNQMLAELEQSHVGLQTALESQ
                     RRFVADASHELRTPMTAIRTNVEFLARVPGAREEDRSAALLDVLSEMRRMETLVGDLL
                     ALARLEATGARPARRAIRLDHLLADIHRDASRLAAGGVEVRLGPTTEVWVSGDRDDLR
                     RAVWNLVENALKYTREGSVDLRLRVGDGTAQLEVADTGIGIAEADHARVFDRFWRAPG
                     TRGAPGSGLGLAITKWVAQAHGGSVLLESAPGRGTTFTLQLPATEERRDARRLQPAGA
                     DGLARVGQPADAAHS"
     gene            3816..4841
                     /locus_tag="VEP73_05045"
     CDS             3816..4841
                     /locus_tag="VEP73_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT25832.1"
                     /translation="MTNDQPRLAAVSQATTPHGQLESALFEIKRVIVGQEGMLERVLI
                     ALLARGHLLLEGVPGLAKTLTISTVADVLGGAFHRVQFTPDLVPSDLVGTRIYRPDSG
                     SFDTELGPVFCNFLLADEINRAPAKVQSALLEVMQERQVTIGHTSYPVGDPFLVLATQ
                     NPIESEGTYPLPEAQVDRFMMKVVVDYPNFSEEMAVVQRSLADRPGVNRVLTLEQLHQ
                     LQRATASVYVDRLVAEYAVALCMATRYPKQYNVGQLAPYVAYGASPRGSINLVHAARA
                     LALLRGRAYTLPQDVQEVAKDVLRHRLVLTYQALAEEVSADALLTAVLTAIPAPRIDL
                     AHEPTAS"
     gene            4838..5803
                     /locus_tag="VEP73_05050"
     CDS             4838..5803
                     /locus_tag="VEP73_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYT25833.1"
                     /translation="MSASTGGPPIAVERTPDRPGPGPTPEALLRALDIEVRRRVEGLL
                     AGEYRSASVGIGTELAQIRPYQPGDDVRRIDWNATARTNEPHTRVEVAERALSSWLVL
                     DTSPSMAFGTADRRKTDVAEGVALAVGHVATRRGNRLGVVTFGDANPQSLPPRQGRAG
                     LLGLLATLRREPMMEGSGATSLGDALARAGRFARTRSVVFVVSDFRGPRDWKAPLLAL
                     AGRHDVIAVEVRDQREQELPSVGELWLVDPETGRHLRVDTSSRALRERFAAAAAAERK
                     ELADALRGAGCDHVVLWTAGDWLATLGGFLRRRGNQWKGGGGARR"
     gene            5834..6880
                     /locus_tag="VEP73_05055"
     CDS             5834..6880
                     /locus_tag="VEP73_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT25834.1"
                     /translation="MSVLWPGFLWALVLVPLAMVAYLLAQRRRAAYAVRFTNLDLLAN
                     VVTRSPGWRRHLPALLYLLALSTLLVSLARPQSVVLVPKEQATVMLVIDVSGSMNATD
                     VEPTRLLAAQQAANSFVNNLPAKFRVGLVSFSATAQTLTRPTTDREAVRDALSSLHAE
                     GGTAMGDAIERGLEAKRPPAPQVDKPPKGSKPGGQRPTPTTSAPPAEAQPAGKEPPVV
                     MLLLSDGANTVGRKQPLEAAEDARRLHVPIFTIALGTDSGVVEVPDESGQPRRIPVPP
                     DKVTLRRVADTTGGKFFTAPSSRDLKGVYRDLGSRIGFVKERQEVTVVFAAAALLLLV
                     AGGALSLAWFNRFP"
BASE COUNT          925 a         2664 c         2483 g          927 t
ORIGIN      
        1 atctgcttcc tcgccggcaa ggcgtcgctc ccccagcccg cgcccccgcc caccttcgaa
       61 ccgatgatgg ccgccctcga gaccgcgccc ttcgatgccc ggggctggct gttcgaggtg
      121 aagtgggacg ggcaccgctg cctggcgaac ctcggcaccg ccacccgcct cacctcccgc
      181 accggccgcg acgcgaccgc ccagttccct gagctgggcg agatgcacag gcagctcgcg
      241 gccaggaacg cggtggtcga cggcgaggtg gtggccctgg acgcggaggg acggccgtcg
      301 ttcgcgcgca tgcaggaccg cttccaccgc tccgccgagg agctggcccg caacgctggg
      361 cgggtgccgg tccagttcat cgccttcgac ctgctctggc tcgacggcgt gcccctcctc
      421 gacctgccgc tgtccgagcg gcgggcgctc ctcgccgagg tgttcgtcga gaccccgcgg
      481 atgcgcctct cccaggtggt ggaggaggcc ggcgccgcct tcttcgccga gatcgagaag
      541 ctcgggctcg agggggtggt cggcaagcgg ctggcgagcc cgtaccgccc cgggcagcgg
      601 tcgcccgact ggcgcaaggt caaggcggtg cgcaggcagg actgcgtgat cgtcggctgg
      661 acgccgggca agggcgcccg tgcggcgacc ctcggggcgc tcctgctcgc cgtctgggac
      721 aatggccgcc tgcgctacgc gggcaacgtc ggcaccggct tcacccaggg gttcctcacc
      781 gagctgctgg ccgacctgcg cgagcgggag acgggcgagc ggccggtcga tccggaccct
      841 cgcgtccgcg gcgcccgctg ggtccggccc gagctggtgt gcgaggtgga gcaccagggc
      901 tggaccaagg agggccggtt acggttcgcc tcgttcaagg ggatcaggat ggacaagcgg
      961 cccgaggact gcctgcccga ggtggccgcc acgggacccg ccgggggccg ggagtaggca
     1021 agatggggac cagcaggcag aatggccctg caaagaggct cagcgaccat cgcagctcag
     1081 agcgctcgca ccgtcgcgac gcctcttcag cggcgaggta aggacaggtt gcatgggtgc
     1141 tcacatcctg gtcgtcgacg acgacccgct cctggcggcc gccctccgcc ggccgctcgc
     1201 gtacgagggc ttcgaggtcg aggtggccgc ctctggcgag gaggcgctct cccgcgcgct
     1261 cgagcgcgcc cccgacctcg tcatcctcga cgtcctcctg ccaggcatcg acggcctcga
     1321 ggtgtgccgg cggctgcggc aggtcgggga cgccgcggtg ctcatgctga cggcccgcag
     1381 cgaggtgccc gagcgggtgg ccggcttcga ggccggtgcc gacgactacc tgggcaagcc
     1441 gttcgccttc gaggagctgc tcgtccgggt gaaggcgctc ctgcggcggg gccgcaacgg
     1501 cggcgcgcca acggcggagc tgcgcttcgg caacgtccgc atggaccggt cgacccgcga
     1561 ggtctggcgc ggcgaccgta cgatctcgct gaccaggcag gagttcgacc tgctcgccct
     1621 gttcctcgag catccgcgcc aggtcctgcc ccggtcgacg atcctcgagc gggtctggga
     1681 ctacgacttc ggccgcgact cgaacgtgct cgaggtgtac gtcggctacc tgcgccgcaa
     1741 gctcgagacc gacgacgagc cgcgggtgat ccacaccgtc cgcggggtcg gctacgtgct
     1801 ccgcgaggac cccgaggcgg cacggtgacc ggcggcggga cgaccctgat gagcttccgg
     1861 gcccggctca cgctggccta cctggcgctg ctcacgctga ccctgtccgc gttcgggttc
     1921 ggcgtctacg cctacgtgga cgccaagctg caccacgagt tcgagtccgc gttcctcacc
     1981 cagggccggg ccctcggcca gctcctgcag gggtactcct cggtcaacac caacggcgtc
     2041 cgctcgttca tgcagcaggt ccgcccgtcc acctactacg tcgtgaccgc caggcgcacc
     2101 cccaaggacc cgtactaccc ggacgtgaac gtccggagcc aggagctgac cggcacggac
     2161 gtgaacctgc ccggcatccc ggccgaccgg aaggtccaca cggtcccgga gcggcagaac
     2221 gagctcgcca cggcgctcgc ggtgtactcg gccgacttcg aggccgtcga gccccccgcg
     2281 tccccccagg gcggggggcg ggcgcagtcg gacacgacgc ccgcgcggtg gcagggccgg
     2341 ctggtgctgg cccgcgacct gaacagcgtc gaggcgtcgc tcagcctgct gcgcaagatc
     2401 ctcatcgccg gcgggctcgc ggtcctcggc ctggccaccc tgatcggctg ggtgctggcg
     2461 gcggggctgc tccggcccct cgacaggatg aagtcgaccg cgcagcggat cggcgacgac
     2521 cgcgacttca cgcgccgcat gcccgtcgac aacgcctacg acgagctcgg ccgcctctcg
     2581 acctcgttca accagatgct ggccgagctc gagcagtcgc acgtgggcct gcagaccgcg
     2641 ctcgagtcgc agcgccggtt cgtcgccgac gcctcccacg agctgcgcac gcccatgacg
     2701 gcgatccgca ccaacgtcga gttcctcgcc agggtcccgg gcgcgcggga agaggaccgg
     2761 tccgccgccc tgctcgacgt gctgagcgag atgcggcgca tggagacgct cgtcggcgac
     2821 ctgctcgccc tcgcccggct ggaggccacc ggcgcccggc cggcccgcag ggccatccgg
     2881 ctcgaccacc tgctggccga catccaccgc gacgccagcc gcctggccgc cggcggcgtc
     2941 gaggtccgcc tcggccccac caccgaggtg tgggtctccg gcgaccgcga cgacctgcgc
     3001 agggcggtct ggaacctggt cgagaacgcg ctcaagtaca cccgcgaggg ctcggtggac
     3061 ctgcgcctgc gggtcggcga cggcacggcc cagctcgagg tcgccgacac cggcatcggc
     3121 atcgccgagg ccgaccacgc gcgcgtgttc gaccgcttct ggcgtgcccc cggcacccgg
     3181 ggcgcgccag ggtccggcct cgggctcgcg atcacgaagt gggtggcgca ggcgcacggc
     3241 ggcagcgtgc tgctcgagtc ggcccccggc cgcggcacga ccttcacgct gcagctcccg
     3301 gccaccgagg agcggcggga cgcgcgccgg ctgcagcccg ctggagcgga cgggctggca
     3361 cgggtgggcc aaccggccga cgccgcacat tcttaagtcg tccctacatg tttctcatct
     3421 agttctcagg gtcgccggat acaactcaac cgctgcggcg gacgcgggcg gtgcggccct
     3481 cggtccctgc cccggacgcc gccgacggtg acgccggagg cccggacggt gggacgaggc
     3541 cggagcaccg ggctccggcc tcggggcctc cggtgagacc gtcggccggc gaacctcggt
     3601 cgcggcgcgt cgcgggccgg cacgcccgga acctaagcct ggtcctgcgg gttccggcgc
     3661 gtcatccttg gtcgtgcccg atcatgcctt cggcgctaag gtgaggatgg ccgcgtttcg
     3721 gcggcgcgtt cgggagccac catccatctc atctcggact catcctcgga gcggtcgcgc
     3781 ggccggctcc ggcgtaccgc aagagggagc cgaccgtgac gaacgaccag ccccggttgg
     3841 ccgcggtgag ccaggcgacg accccgcacg gccagctcga gagcgcgctg ttcgagatca
     3901 agcgggtgat cgtcgggcag gagggcatgc tcgagcgcgt cctcatcgcc ctgctcgccc
     3961 ggggccacct gctcctcgag ggcgtgccgg ggctcgccaa gacgctcacc atctccaccg
     4021 tggccgacgt gctcggcggg gccttccacc gggtccagtt cacgcccgac ctggtcccct
     4081 ccgacctcgt cggcacccgc atctaccggc ccgactccgg cagcttcgac accgagctgg
     4141 gccccgtgtt ctgcaacttc ctgctcgccg acgagatcaa ccgcgcgccc gccaaggtgc
     4201 agtcggccct gctcgaggtc atgcaggagc gccaggtcac catcggccat acgagctacc
     4261 cggtcggcga ccccttcctg gtcctcgcca cccagaaccc gatcgagtcc gagggcacct
     4321 atcccctgcc cgaggcccag gtggaccggt tcatgatgaa ggtggtcgtc gactacccga
     4381 acttcagcga ggagatggcg gtcgtccagc gctcgctggc cgaccgtccc ggcgtcaacc
     4441 gggtcctcac gctcgagcag ctgcaccagc tccagcgggc gacggcgtcc gtctacgtcg
     4501 accggctggt ggccgagtac gccgtcgccc tgtgcatggc gaccaggtac cccaagcagt
     4561 acaacgtcgg ccagctcgcc ccctacgtcg cctacggggc gagcccccgg gggtcgatca
     4621 acctcgtcca cgccgcgcgg gccctcgcgc tgctccgggg ccgcgcctac acgctccccc
     4681 aggacgtcca ggaggtcgcg aaggacgtgc tccgccaccg cctggtcctg acctaccagg
     4741 cgcttgccga ggaggtcagc gcggacgcgc tgctcaccgc cgtgctgacc gcgatcccgg
     4801 cgccccgcat cgacctggcc cacgagccga ccgcgtcatg agcgcgagca ccggcggccc
     4861 gccgatcgcc gtcgagcgca cgcccgaccg gccgggaccc gggccgaccc cggaggcgct
     4921 gctgcgcgcc ctcgacatcg aggtccggcg ccgggtcgag gggctgctcg ccggggagta
     4981 ccggtccgcc tcggtcggga tcggcaccga gctcgcccag atccgcccct accagcccgg
     5041 cgacgacgtc cggcgcatcg actggaacgc gaccgcccgc accaacgagc cccacaccag
     5101 ggtcgaggtg gccgagcggg cactgtcgag ctggctcgtg ctcgacacct cgccgtcgat
     5161 ggccttcggc accgccgacc ggcgcaagac cgacgtcgcc gagggggtcg ccctggccgt
     5221 cggccacgtc gccacgagaa ggggcaaccg tctcggggtg gtgacgttcg gggacgcgaa
     5281 cccgcagtcg ctcccgcccc gccaggggcg ggccgggctg ctcgggctgc tcgcgacgct
     5341 ccgcagggag ccgatgatgg agggcagcgg cgccacctcg ctcggcgacg ccctggcgcg
     5401 ggccggccgc ttcgcccgca cccgctcggt ggtcttcgtc gtgagcgact tccgcgggcc
     5461 gcgtgactgg aaggcacccc tgctcgccct cgccgggcgg cacgacgtga tcgcggtcga
     5521 ggtccgcgac cagcgcgagc aggagctccc gagcgtcggg gagctgtggc tggtcgaccc
     5581 cgagaccggc cgccacctgc gggtcgacac ctcgagccgg gcgctgcggg agcgcttcgc
     5641 cgccgcggcc gccgccgagc ggaaggagct ggccgacgcg ctgcggggcg ccggctgcga
     5701 ccacgtcgtc ctctggacgg ccggggactg gctcgccaca ctgggcgggt tcctccgcag
     5761 gcgcggcaat cagtggaagg gtggaggagg ggcgcgccgg tgaccgggat cataccgaga
     5821 tgagcgtcct gtgatgagcg tcctgtggcc cggattcctc tgggcgctcg tgctggtgcc
     5881 gctcgccatg gtcgcgtacc tgctcgccca gcgccgccgg gccgcctacg cggtccgctt
     5941 caccaacctg gacctcctgg ccaacgtggt cacccgctcg cccggctggc gccgccacct
     6001 gccggccctg ctctacctgc tcgcgctctc gacgctgctc gtcagcctgg cccgccccca
     6061 gtcggtcgtg ctcgtgccca aggagcaggc cacggtcatg ctcgtcatcg acgtctcggg
     6121 ctcgatgaac gccaccgacg tggagccgac gcgcctgctg gccgcccagc aggccgcgaa
     6181 ctcgttcgtc aacaacctgc cggccaagtt ccgggtcggc ctggtgtctt tctcggccac
     6241 ggcccagacc ctcacccgcc cgaccaccga ccgcgaggcc gtccgggacg cgctcagctc
     6301 gctccacgcc gagggcggca ccgcgatggg cgacgccatc gagcgtggcc tcgaggccaa
     6361 gcggcccccg gccccgcagg tggacaagcc ccccaagggc agcaagccgg gcggccaacg
     6421 gccgaccccg acgacgagcg cgcccccggc cgaggcgcag cccgcgggca aggagccccc
     6481 ggtcgtgatg ctcctgctgt cggacggcgc caacaccgtg ggcaggaagc agccgctgga
     6541 ggcggccgag gacgccaggc ggctccacgt ccccatcttc acgatcgcgc tcggcacgga
     6601 ctcgggcgtc gtcgaggtgc ccgacgagag cggccagccg cgccgcatcc ccgtgccgcc
     6661 cgacaaggtg accctgcggc gcgtcgccga caccaccggg gggaagttct tcaccgcgcc
     6721 gagcagccgg gacctcaagg gtgtctaccg ggacctcggg tcgcgtatcg gcttcgtgaa
     6781 ggagcggcag gaggtgaccg tcgtcttcgc cgccgcggcg ctgctcctgc tggtcgccgg
     6841 gggggcgctg tcgctggcct ggttcaaccg gttcccctga gcggcggggc gggcccggcg
     6901 tccgtctcct cgggcgcgac ggccgcgccg tcgagctcct cggcccgctc ggacacctcg
     6961 gcgagccggc cgtcgaagcg gtcgagccgg cgcgcggcc
//
LOCUS       DASPYY010000328         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2304323, whole genome shotgun sequence.
ACCESSION   DASPYY010000328 DASPYY010000000
VERSION     DASPYY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304323"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="VEP73_05060"
     CDS             <1..2424
                     /locus_tag="VEP73_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25835.1"
                     /translation="ELGSVRVRDRFEDELSDAMVRSNDGDVRAGGRGALFIKGTMGGQ
                     YLLTARYDSQEDPRRRLFRDIRPDEYYPVYGDASVRTFEAQSTGRVYAKLERDQSYAM
                     YGDYVTPGNQDLQLLGAYHRSFEGGVTHLEQGPVSVDAYGTWSHSSQVVDEIPAEGVS
                     GPYTLRRSGALVNSEQVEILTRDRNQPSIIVKREPKQRYTDYVIEPLTGNLIFRAPVP
                     SVDQYLNPVSIRVTYEVEGGGDRFWTYGANGLVTVVPGIQLGGSASRDEDPVAGRDIY
                     GGQARLGLTRFVEAIGELAVSDSSGRRGEAERVELRHHSAHLDGRAWGLWSDLDFRNP
                     SSGFGTGRRELGASGRAPLDGKTSVFGEGLRTEDVINGGRRDGWMAGIDRRLASRLKL
                     EVAYRFARETPLPASAATLGATPNETQALRGRIDGGMPGVPRLNLYGEYEQDLQKDDQ
                     HRAAVGGEFKLAERVKLYGRTELLSSFAGPYALNGVQEQASTVVGISADGFHDTRVFS
                     EYRARDAFNGRETQAAIGLNNHWTPSPGVGLETAFERLTILEGQGAGDATAVSGGVEF
                     TGSPLWKGSARLEYRTATGNDRWFGTLGYAQKLSRDWTLLGRSAFTLLPDQDHTDERS
                     QLGLAFRQTDVNHWNALMRYTHHLEDDQPAGGPVARTEAHVVSTHVNLQPSRPWLVES
                     QLAAKWARDDHDGLDTHTNVQLAGGRVLYDLTSRWDVGASARGLFSRDAASRQYGVGG
                     ELGRVLAPNLRLAVGYNLFGFRDQDLAGESYTDQGPYFHFGFKFDESMFGFGPPRSTG
                     TTGHSGKGN"
     gene            2426..3565
                     /locus_tag="VEP73_05065"
     CDS             2426..3565
                     /locus_tag="VEP73_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYT25836.1"
                     /translation="MRHHGSDEGRRRYGCAGVLALLAAQVLAAGVGPARAADKARAAV
                     AQTAAPGALTPLGRRVSDEGIARDLRLTEALARRLEAHTSGPSPADPYRASRARALIL
                     LTREEYLDNDPTAIADAAYAQAVATVEEVERGTPGLAPALPILAGSKQVRADLWQVVT
                     EVRNSAGLHCAADLLGRLEVELEWAGHEELDCGTCRSNSHIAIAEQLAAAARRLADSC
                     ATPPPPPPPPPPVVVVPPPPPPKPVERETTIVSVPNVVHFAYNVSTVGPATSAVLQQV
                     SAMMVKNPGVSLHLVGHTDSRGSVEYNMKLSRKRAEAVRDFLVTAGVEDGRLGIGWEG
                     KSRPLRLAAGVRDFAYNRRVEFVFTVPDTLKVRTGEQDKDLQPDR"
BASE COUNT          535 a         1333 c         1326 g          464 t
ORIGIN      
        1 gagctcggca gcgtccgggt gcgcgaccgg ttcgaggacg agctctccga cgccatggtc
       61 cgcagcaacg acggcgatgt ccgggccggt ggccgcggcg ccctgttcat caagggcacc
      121 atgggcggcc agtacctgct caccgcgcgc tacgactcgc aggaggatcc gcgccgccgc
      181 ctgttccgcg acatccggcc cgacgagtac tacccggtct acggcgacgc ctcggtgcgg
      241 accttcgagg cccagtcgac cggccgggtc tacgccaagc tggagcgcga ccagtcctac
      301 gccatgtacg gcgactacgt cacgcccggc aaccaggatc tccagctgct gggcgcctac
      361 caccgcagct tcgagggtgg cgtgacccac ctcgagcagg gcccggtgtc ggtggacgcg
      421 tacggcacct ggtcgcactc gagccaggtg gtggacgaga tcccggccga gggcgtgtcc
      481 ggaccctaca cgctgcgccg ctcgggagcg ctggtgaaca gcgagcaggt ggagatcctg
      541 acgcgcgacc gcaaccagcc gtcgatcatc gtcaagcgcg agcccaagca gcgctacacc
      601 gactacgtca tcgagccgct gaccgggaac ctcatcttcc gggcgccggt gccgagcgtg
      661 gaccagtacc tgaacccggt ctcgatccgc gtcacctacg aggtcgaggg cggcggcgac
      721 cggttctgga cctacggcgc caacggcctc gtgaccgtgg tcccgggcat ccagctcggc
      781 ggctcggcgt cgcgcgacga ggatccggtg gccgggcgcg acatctacgg cggccaggcg
      841 cgcctgggcc tgacccggtt cgtggaggcg atcggcgagc tggcggtgtc ggatagcagc
      901 ggccggcgcg gcgaggccga gcgcgtcgag ctgcggcacc actcggcgca cctcgacgga
      961 cgcgcgtggg gcctgtggag cgacctggac ttccgcaacc cgtcgtcggg cttcggcacc
     1021 ggtcgccgcg agctcggcgc gtcgggccgc gccccgctcg acggaaagac ctcggtgttc
     1081 ggcgaggggc tgcggaccga ggacgtgatc aacggcggcc gtcgcgacgg ctggatggcg
     1141 gggatcgacc gccgcctcgc gtcgcggctc aagctggaag tggcctaccg cttcgcccgc
     1201 gagaccccgt tgccggcctc ggcggccacc ctcggcgcca cgccgaacga gacccaggcc
     1261 ctgcgcggcc ggatcgacgg cggcatgccg ggggtcccgc ggctcaacct gtacggcgag
     1321 tacgagcagg atctccagaa ggacgaccag caccgcgccg ccgtgggcgg ggagttcaag
     1381 ctggccgagc gcgtgaagct ctacggccgg accgagctgc tgtcctcgtt cgccggaccc
     1441 tacgcgctga acggcgtgca ggagcaggcg agcacggtgg tcgggatctc ggccgacggc
     1501 ttccacgaca cccgggtgtt cagcgagtac cgggcgcgcg atgccttcaa cggccgcgag
     1561 acccaggcgg cgatcgggtt gaacaaccac tggacgccga gccccggcgt cggtctcgag
     1621 accgcgttcg agcgcctgac gatcctcgag ggccaggggg cgggcgatgc gacggcggtc
     1681 tccggagggg tcgagttcac cggcagcccg ctgtggaagg gctcggcgcg cctcgagtac
     1741 cgcaccgcca ccggcaacga ccgctggttc ggcacgctgg gctacgcgca gaagctgtcg
     1801 cgcgactgga cgctgctcgg ccgcagcgcg ttcacgctgc tgcccgacca ggatcacacc
     1861 gacgagcgct cgcagctcgg cctggcgttc cgccagaccg acgtcaacca ctggaacgcc
     1921 ctgatgcgct acacgcacca cctcgaggac gaccagccgg ccggcggccc ggtggcccgg
     1981 accgaggccc acgtggtctc gacccacgtg aacctgcagc cctcgcggcc gtggctggtc
     2041 gagtcccagc tggccgccaa gtgggcgcgc gacgatcacg acggcctgga cacccacacc
     2101 aacgtccagc tcgccggcgg ccgcgtcctc tacgacctga cctcgcgctg ggacgtgggc
     2161 gcgagcgcac gcgggctgtt cagccgcgac gccgcgagcc gccagtacgg ggtcggcggc
     2221 gagctcggcc gggtgctcgc gccgaacctg aggctggcgg tgggctacaa cctgttcggg
     2281 ttccgcgacc aggatctggc gggcgagtcg tacaccgatc agggcccgta cttccacttc
     2341 gggttcaagt tcgacgagag catgttcggg ttcgggccgc cgcgctcgac cggaaccacg
     2401 ggccacagcg ggaaggggaa ctgagatgcg ccatcacggc agcgacgaag ggcggcgccg
     2461 gtacgggtgc gcgggcgtgc tcgccctgct ggccgcgcag gtgctcgcgg ccggggtggg
     2521 gcccgcgcgg gcagcggaca aggcgcgggc ggccgtggcg cagaccgccg ccccgggggc
     2581 gctgacgccg ctcggccggc gcgtcagcga cgagggcatc gcccgcgacc tgcgcctcac
     2641 cgaagcgctg gcgcggcggc tcgaggccca cacgtcgggc ccgtcgcctg ccgaccccta
     2701 ccgggcgtcg cgggcgcgcg cgctcattct gctcacgcgc gaggagtacc tggacaacga
     2761 tccgaccgcg atcgccgacg cggcctacgc ccaggccgtc gcgacggtcg aggaggtcga
     2821 gcgcggcacc cccgggctcg cgccggcgct gccgatcctc gccggttcga agcaggtccg
     2881 ggccgatctc tggcaggtcg tcaccgaggt gcgcaactcg gccgggctcc attgcgcggc
     2941 cgacctgctc ggccggctcg aggtggagct cgagtgggcg ggacacgagg agctcgactg
     3001 cggcacgtgc cggagcaact cgcacatcgc gatcgccgag cagctcgcgg ccgcggcccg
     3061 ccggctcgcc gactcctgcg cgacgccgcc gcccccgccg ccgcctccgc cgccggtcgt
     3121 ggtcgtgccg ccgccgcccc cgcccaagcc ggtggagcgc gagaccacga tcgtcagcgt
     3181 gccgaacgtc gtccacttcg cctacaacgt ctccacggtc ggcccggcga ccagcgcggt
     3241 gctccagcag gtgtccgcca tgatggtcaa gaaccccggc gtctcgctcc acctggtcgg
     3301 gcacaccgac tcgcgcggct cggtcgagta caacatgaag ctgtcacgca aacgcgccga
     3361 ggcggtgcgg gacttcctgg tcaccgcggg agtcgaagac ggacggctcg ggatcggctg
     3421 ggagggcaag agccggccgc tccgcctggc cgccggtgtc cgggacttcg cctacaaccg
     3481 gcgcgtcgag ttcgtcttca ccgttcccga cacgctcaag gtgcgcaccg gggagcagga
     3541 caaggacctt cagcccgacc ggtagcccgg cgggtgcaga tcaagaccgc ggcgtggcga
     3601 ttcccgttcg ccacgccgcg atgcgtttaa ggccggcgcc gtcaccaagg agaaggtc
//
LOCUS       DASPYY010000329         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_898831, whole genome shotgun sequence.
ACCESSION   DASPYY010000329 DASPYY010000000
VERSION     DASPYY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898831"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VEP73_05070"
     CDS             complement(<1..563)
                     /locus_tag="VEP73_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT25837.1"
                     /translation="MLVVGAILAAAAGAAYNTTAAIQKHEVVRASTPARRLLPALARR
                     PRWLATLVLDLAAWTAQTAAMALAPLVLVVPLMALGAALLVVLGVGWLGERFGRGELL
                     AVALIVAGSAMVALAAGRLTVARTPLAPATQLLVAGIALGAALVAMGGRTGIAYGTAA
                     GCLYAATAVLSKEIGDRVADHGLRALPL"
     gene            602..2566
                     /locus_tag="VEP73_05075"
     CDS             602..2566
                     /locus_tag="VEP73_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT25838.1"
                     /translation="MVGEAGPRRVLLLTAEMGEGHNAAARALGRTAEELWPGVELRFL
                     DAIRQMGPGMSWAAQATYRFALTRTPWVYQFFYDALWRHPWFAGVNKRLIAAWCGRCL
                     RRRVRRYRPDLVACTHPFGAAAMDWLRHSGGYAAPTVSFVTDFAPHPFWVYPRIDLHL
                     VLHELGAAQVRRLGGDRVRVAALPVAPAFHPRDRHAERRRLGLAHQDQDAFVALVTGG
                     AWGVGTLEDAVRVLLRDGGQVQVVAVCGRNEELRIRLEALGAPLERLVVLGFVDTMPA
                     LLAACDVVVTNAGGLTALEAFASARPVVLFDPIAGHGRANAALMERAGLAVVCHGTAE
                     LRATIAELAAGGVLAARLRETARLVAGRDLADDVRLLASTALPVPFGTPRRASAFSVP
                     FGTPRRASETPREPRRPPLRVAVRLAVAMLLAVLVASWGSYVAGTRLAPAARGAPAPS
                     GAVAITLAGSLPPAVLRAVARAAGEAGVPLTFFVEGADAAADAALLASLERQGLEVEA
                     GTWRAGRDDQLRPGRVYAEFSRTLDAVTRADRHRPRYVAEPDGQFSLVAVATTGDLHL
                     RRVVFGWRTTVPPRVDSLPPLAPGQIVELLVLPTASAVQVQATVLTVAAAARGRGLRL
                     ATVDAIDRAGRAPASDRRAADTLRAEVAVA"
     gene            2563..>3207
                     /locus_tag="VEP73_05080"
     CDS             2563..>3207
                     /locus_tag="VEP73_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYT25839.1"
                     /translation="MTRARSLVRAGAGAAGVAAAAAAGYWGVGAAGAPPLRRVLRRSV
                     LSRMPVGEPLLALTFDDGPDPGLTERFLAALGGTPATFFPLGERVRRWPHLARMVAAA
                     GHEVACHGDTHRSLAVLPPGATVDALRRAQGSIGDACGVAPRFFRPPYGTFNLAAWLA
                     APRLGMRRTLWSAWARDWEEQATPELIAARLLRGAAPGAILLLHDADGSPGAPRR"
BASE COUNT          330 a         1217 c         1256 g          404 t
ORIGIN      
        1 agcggcagcg ctcgcaggcc atggtcggcg acccggtcgc cgatctcctt ggacagcacg
       61 gcggtggccg cgtacaggca gccggcggcc gtgccgtagg cgatgccggt gcgcccgccc
      121 atcgcgacca gggcggcgcc gagcgcgatg ccggcgacga ggagctgggt ggcgggcgcg
      181 agcggggtgc gtgcgaccgt gagccggccg gcggcgagcg cgaccatcgc gctcccggcg
      241 acgatcagcg cgaccgcgag cagctcgcca cgcccgaacc gctccccgag ccagcccacc
      301 cccagcacga ccagcagcgc cgcgccgagc gccatcagcg ggacgaccag caccagcggc
      361 gccagcgcca tcgcggcggt ctgtgcggtc caggcggcca ggtccagcac cagcgtcgcg
      421 agccaccgtg gccgccgggc gagcgcgggc agcagccgac gggcgggggt ggaggcccgc
      481 accacctcgt gcttctggat ggctgcggta gtgttgtagg cggctcccgc ggccgctgcg
      541 aggatcgcgc cgacgacgag cacgtggtct ccatgccgac aatggggagc cgaaccaggc
      601 gatggtaggc gaggccggcc cccgacgtgt gctgctcctg accgccgaga tgggcgaggg
      661 gcacaacgcc gcggccaggg cgctgggccg cacggccgag gagctgtggc ccggcgtcga
      721 gctgcgcttc ctcgacgcga tccggcagat gggccctggc atgtcgtggg cggcgcaggc
      781 gacctaccgc ttcgccctga cccgcacccc ctgggtgtac cagttcttct acgacgcgct
      841 ctggcggcac ccctggttcg ccggggtgaa caagcggctc atcgccgcct ggtgcggtcg
      901 ctgcctgcgc cgacgggtgc gccgctaccg tccggacctg gtcgcctgca cgcacccgtt
      961 cggcgcggcg gcaatggact ggctgcgcca cagcggcggc tacgccgcgc cgacggtgtc
     1021 gttcgtcacg gacttcgcgc cgcacccgtt ctgggtgtac ccgcggatcg acctgcacct
     1081 ggtgctgcac gagctgggtg cggcgcaggt gcggcggctc ggtggcgacc gggtgcgggt
     1141 cgcggcgctg ccggtcgcgc ccgcgttcca cccgcgcgac cggcacgccg agcggcgtcg
     1201 cctcggtctg gcgcaccagg accaggacgc gttcgtcgct ctggtcaccg gtggcgcctg
     1261 gggcgtcggc acgctcgagg acgcggtgcg cgtgctgctc cgcgacggcg ggcaggtgca
     1321 ggtcgtggcg gtgtgcggcc gcaacgagga gctcaggatc cgcctggagg cgctcggcgc
     1381 gccgctggag cgcctggtcg tgctgggctt cgtggacacg atgcccgcgt tgctggccgc
     1441 ctgcgacgtg gtcgtcacca acgcgggcgg gttgaccgcg ctggaggcgt tcgcctccgc
     1501 gcgcccggtc gtgctgttcg accccatcgc cgggcacggc cgcgcgaacg cggcgttgat
     1561 ggagcgcgcc gggctggcgg tcgtctgcca cggcaccgcc gagctgcgcg ccaccatcgc
     1621 ggagctggcc gcgggcggcg tgctggcggc acggctgcgc gagacggcgc ggctggtggc
     1681 cggccgcgac ctggcggacg acgtgcgcct gctcgcctcg accgctctcc ctgtcccgtt
     1741 cggcacgccg aggcgtgcct ccgccttctc tgtcccgttc ggcacgccga ggcgtgcctc
     1801 cgaaacgccg cgcgagccac ggcggccacc gctccgggtc gcggtccgtc tcgccgtcgc
     1861 gatgctgctg gccgtgctcg tggcctcctg ggggtcgtac gtcgcgggca cgcgcctcgc
     1921 ccccgccgca cgtggcgcgc cggcaccgtc gggggcggtg gcgatcaccc tggccggatc
     1981 gctgcccccg gcggtgctgc gcgcggtcgc gcgggcggcg ggcgaggcgg gtgtgccgct
     2041 gacgttcttc gtcgagggtg ccgacgccgc cgcggacgcc gccctgctcg cctccctgga
     2101 gcggcaagga ctcgaggtgg aggcgggaac ctggcgggcg ggtcgcgacg accagctccg
     2161 ccccggccgg gtctacgccg agttcagccg cacgctggac gcggtgacca gggcagaccg
     2221 ccaccgcccc cgctacgtgg ccgagccgga cgggcagttc agcctggtcg ccgtggccac
     2281 caccggagac ctgcacctgc gccgcgtggt cttcggctgg cggacgacgg tcccaccgcg
     2341 ggtggactcg ctgccaccgc tcgcccctgg gcagatcgtg gagctgctgg tgctgccgac
     2401 cgcctcggcg gtgcaggtgc aggccacggt gctgacggtc gccgcggcgg cgcgcgggcg
     2461 gggcctgcgg ctcgccaccg tcgacgcgat cgaccgggcc gggcgagcgc ccgcctccga
     2521 ccggcgtgcc gccgacacgc tgcgggcgga ggtggcggtc gcatgacccg ggcgcggtcg
     2581 ctggtccgcg ccggcgccgg cgcggcgggc gtggcggccg cggcggccgc cggctactgg
     2641 ggggtcgggg cggccggggc gccgccgctg cggcgggtgc tccgtcgcag cgtgctgtcc
     2701 cggatgccgg tcggggagcc gctgctggcg ctcaccttcg acgacggccc cgacccgggg
     2761 ctcaccgagc ggttcctcgc cgcactcggc ggcaccccgg ccacgttctt cccgctcggc
     2821 gagcgcgtgc gccgttggcc gcacctggca cggatggtcg ccgcggccgg gcacgaggtc
     2881 gcctgccacg gagacaccca ccgcagcctg gccgtgctgc cgccaggcgc caccgtcgac
     2941 gcgctgcggc gcgcccaggg gtcgatcggg gacgcgtgcg gggtcgcacc ccgcttcttc
     3001 cgtcccccgt acggcacctt caacctggcc gcctggctcg cggcaccgcg gctcggcatg
     3061 cgccgcaccc tgtggagcgc ctgggcgagg gactgggagg agcaggccac ccccgagctg
     3121 atcgccgcac gcctgctgcg gggtgccgcg cccggcgcga tcctgttgct gcacgacgcc
     3181 gacggcagcc ccggcgcgcc gcgccgc
//
LOCUS       DASPYY010000330         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_479348, whole genome shotgun sequence.
ACCESSION   DASPYY010000330 DASPYY010000000
VERSION     DASPYY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479348"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VEP73_05085"
     CDS             <1..1419
                     /locus_tag="VEP73_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYT25840.1"
                     /translation="SVAWLAQALQADARGNTRATVLACARGLDALEEHQMRLGATELR
                     AYGTAHGAELATLAQRDALRRGDVRRLLFWSERWRATALAARSTPIREDKELAAELQA
                     LRSVSRILGDTEMAAPRRNALERERRRLEAAVQARTRRSPGGHEPGDGQFNLDGLFDE
                     LGAATLIELVTVDGVLHVVVVADRRVRLHRVGSVPEREVQMNRFVLRRLAHGRRQPGD
                     EIVLKYRGAALEAALLGGAAAGFGDGPIVVIPPAQFRAVPWSLMPSLRDRVVTVAPSA
                     SAWLRARRMRPPSRRRVTLVTGPGLATKGAEVEPLRSRYPRARLLGHGNATAGQVLTA
                     LDGVWLAHIAAHGTFRADNPMFSSILLDDGPLIVHDFDRLGRAPYQMVLAGCDSGVAA
                     PVGADELLGLVSSLMPLGAAGIVASIVPVNDIAVVPVMVALHDALHRGAGLPEALLAA
                     RRASGGDPLAEATAASFLALGA"
     gene            complement(1430..>2031)
                     /locus_tag="VEP73_05090"
     CDS             complement(1430..>2031)
                     /locus_tag="VEP73_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HYT25841.1"
                     /translation="DWPRDADGRIGLAEAVARSRATMLIGTSTQPGAFTEQIVTEMAS
                     HAERPVIMPLSNPTSHSEAQAADLIAWTGGRALIATGSPFAPVPYQGVEYQVAQANNA
                     LIFPGLGLGVTVSRARRVSDGMLAAAARALADLSDAAAPGAAVLPPVTSLRAVSAAVA
                     EAVARAARAEGLSDVPLDGLAERVQAAMWEPAYPKVEAI"
BASE COUNT          245 a          745 c          772 g          269 t
ORIGIN      
        1 agcgtggctt ggctagccca ggccctacag gccgacgccc ggggtaacac ccgggccacc
       61 gtcctggcct gcgcgcgcgg gctcgacgcc ctcgaagagc accagatgcg gctcggcgcc
      121 accgaactgc gcgcgtacgg cacggcgcac ggcgccgagc tcgccacgct ggcccagcgc
      181 gacgcgctcc ggcggggcga cgtccgccgg ctgctgttct ggagcgagcg gtggcgggcg
      241 accgcgctgg ccgcccggag cacaccgatc cgcgaagaca aggagctagc ggccgagctg
      301 caggcgctgc gcagcgtcag ccgcattctc ggggataccg agatggccgc gccgcgccgg
      361 aacgccctgg agcgggagcg gcggcggctt gaggcggcgg tccaggcccg gacgcgcagg
      421 tcgcccggcg gccacgagcc cggggacggc cagttcaacc tggatgggct ctttgacgag
      481 ctcggcgcgg ccacgctgat cgagctcgtc acggtggacg gtgtgctgca cgtggtcgtc
      541 gtcgcggacc ggcgggtccg gctgcacagg gtgggcagcg tgcccgagcg cgaggtccag
      601 atgaaccgct tcgtcctgcg ccgcctcgcc catggccgac ggcaacccgg cgacgaaatc
      661 gtgctgaaat accggggcgc tgccctggag gccgcgttgc tcggcggcgc agccgccggg
      721 ttcggggacg gcccgatcgt ggtgatcccg ccggctcagt tccgggcagt gccgtggagc
      781 ctcatgccgt cgttgcgcga ccgcgtcgtg acggtggcac cgtccgcgtc ggcctggctg
      841 cgcgcccgtc gcatgcggcc gccgtcccgg cggcgcgtca ccctcgtcac cgggcccggg
      901 ctggctacca agggcgcgga ggtcgagccg ctgcgctctc gctatccaag ggccaggctg
      961 ctgggtcacg gcaacgccac agccggccag gtactcaccg cccttgacgg cgtgtggctg
     1021 gcgcacatcg cggcccacgg cacgttccgg gccgacaacc ccatgttctc gtccatcctg
     1081 ctcgatgacg gaccgctgat cgtgcacgac ttcgatcgcc tcgggcgcgc gccgtaccag
     1141 atggtcttgg ccggctgcga ctccggagtc gcagccccgg taggcgccga cgagctcctg
     1201 ggcctggtca gcagcctgat gccgctcggc gcggccggaa tcgtggccag catcgtgccc
     1261 gtcaacgaca tcgccgtggt gcccgtcatg gtggctctgc acgacgctct gcaccgcggt
     1321 gccgggctcc ccgaagccct cctggccgcg cgccgcgcgt cgggcggcga cccgctcgcc
     1381 gaggcgacag cagcctcctt cctggccctc ggcgcctgag gctccgctgt cagatggcct
     1441 ccaccttcgg gtaggcgggt tcccacatgg ccgcctggac ccgttcggcc aggccgtcga
     1501 ggggcacgtc ggacagtccc tcggcccggg ccgcccgggc gacggcctcg gcgaccgcgg
     1561 cggacaccgc ccgcaggctg gtgaccgggg gcagcacggc cgctcccggg gcggcggcgt
     1621 cggacaggtc cgccagcgcg cgggccgcgg cggcgagcat gccgtcgctg acgcggcggg
     1681 cccggctgac ggtgaccccc agcccgaggc cgggaaagat gagcgcgttg ttggcctggg
     1741 cgacctggta ctcgaccccc tggtagggga ccggcgcgaa ggggctgccg gtcgcgatga
     1801 gcgcccgccc gccggtccac gcgatcaggt cggcggcctg ggcctcggag tgcgaggtgg
     1861 ggttggacag gggcatgatg accggtcgtt cggcgtgcga ggccatctcc gtgacgatct
     1921 gctcggtgaa cgccccgggc tgggtcgagg tcccgatcag catggtggcg cggctgcggg
     1981 ccaccgcctc ggcgaggccg atccggccgt ccgcgtcgcg cggccagtcg g
//
LOCUS       DASPYY010000331         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1883581, whole genome shotgun sequence.
ACCESSION   DASPYY010000331 DASPYY010000000
VERSION     DASPYY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883581"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..691)
                     /locus_tag="VEP73_05095"
     CDS             complement(227..691)
                     /locus_tag="VEP73_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HYT25842.1"
                     /translation="MSNMETYGRSAPLARPPARVLAWRPTSTPERLGLTGLAAAGASF
                     VYPAASRATGLTAPCLLRMTTGIPCPFCGMTTASTALAAGDLHAALVANPFVLVLAGT
                     TLVMTVLIAARAAGLAPPAASWQPARQRQAWLAAGLLAVASWLFQLHRFGWI"
     gene            959..1675
                     /gene="purN"
                     /locus_tag="VEP73_05100"
     CDS             959..1675
                     /gene="purN"
                     /locus_tag="VEP73_05100"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYT25843.1"
                     /translation="MADVKRVAVLVSGHGSNLQALMDAAADPSYGARVELVASDRPDA
                     HGLERARAAGIPTAVVRPADFPDRAAFDEALAGLVAAARPDAVCLAGFMRILGPAFVR
                     AFPGRIVNTHPSLLPAFRGAHAVRDALAYGVRVTGCTVHLVDEEVDHGPVLLQAAVAV
                     RDGDDEASLHGRIKREEHRLLPAAVRLVALGRVRLDGRRAWLEGDQPAPPSDPPGRAR
                     PHPGGPQHARAGETTSGGVP"
BASE COUNT          212 a          653 c          650 g          211 t
ORIGIN      
        1 acccgccgcc ctggggcggt acgggcggcg tgggcgagcc ccagccaccg ccgggcgggg
       61 gagggctcgc cggcgcggcc gggtcgtcct gtccccggcg cggctcctcg ggcacgtccc
      121 cccgaggtgg ggttccctcg ctcatgcttc ctcctttagt tgtctcgagc atgttgtctc
      181 gagccgttcg gccggtccag cgccgggcga acgtcgtgcc ggtgcgtcat atccaaccga
      241 acctgtgcag ctggaacagc cagctggcga ccgccagcag cccggccgcc agccacgcct
      301 ggcgctgcct tgccggctgc cagctggccg ccgggggcgc cagaccggcc gccctcgcgg
      361 cgatgaggac ggtcatgacc agcgtcgtcc cggcgagcac gagcacgaac gggttcgcga
      421 cgagggcggc gtgcaggtcg ccggctgcca gcgcggtgga ggccgtggtc atcccgcaga
      481 acgggcacgg gatgcccgtc gtcatgcgca gcaggcaggg cgcggtcagc ccggtcgcgc
      541 ggctggcggc cgggtagacg aacgaggcgc ccgccgccgc caagccggtg aggccgagcc
      601 gctccggcgt cgaagtcggg cgccaggcca ggacgcgcgc gggcggccga gcgagcgggg
      661 cagatcgtcc gtacgtctcc atgttcgaca tgtcggtcct gctcagggtc gtcgcaggca
      721 ctgaccgcac tgaatccgtg cgggtcggcg cagtgaacca catgcacagg gaggcagcaa
      781 tcctcaccac gccgtttgta ccgatccgtt gcgcctgcgc ccctgccggg cgggagccgg
      841 cccggttgcc ggccggggag tcgagtggca gcatccgagc gcggcagccc ccgatcgacg
      901 gcaaggggtt cgggggctca ggcagcgcag cggtagcccc cgatcgatga gggaggacgt
      961 ggcggacgtc aagcgggtgg ccgtgctggt gtccggccac ggctccaatc tgcaggcgct
     1021 catggacgcg gcggccgacc cgtcctacgg ggccagggtg gagctcgtgg ccagcgaccg
     1081 gccggatgcg cacgggctcg agcgggcgcg tgcggccggc atcccgaccg ccgtcgtccg
     1141 accggccgac ttccccgacc gggccgcgtt cgacgaggcc ctggccggtc tggtcgccgc
     1201 cgcgcggccg gacgccgtct gcctggccgg gttcatgcgc atcctcggcc cggcgttcgt
     1261 gcgcgccttc ccggggcgca tcgtcaacac ccacccgagc ctgctgccgg ccttccgggg
     1321 cgcgcacgcc gtgcgcgacg ccctggccta cggcgtgcgg gtcaccggct gcaccgtgca
     1381 cctcgtcgac gaggaggtcg accacggccc ggtgctcctg caggcggccg tcgccgtccg
     1441 ggacggcgac gacgaggcca gcctgcacgg gcgcatcaag cgggaggagc accgcctcct
     1501 gccggccgcc gtccgcctgg tcgcgctcgg ccgggtccgc ctcgacggca ggcgcgcctg
     1561 gctggagggc gaccagccgg cgccccccag cgacccgccc ggccgggcac ggccccaccc
     1621 cggcggtccc cagcacgccc gagcaggcga gacgacctcc ggaggagtgc cgtgaccaac
     1681 cgccagacca tccgccgcgc cctcgtctcg gtgttcgaca aggccg
//
LOCUS       DASPYY010000332         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_618889, whole genome shotgun sequence.
ACCESSION   DASPYY010000332 DASPYY010000000
VERSION     DASPYY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VEP73_05105"
     CDS             complement(<1..584)
                     /locus_tag="VEP73_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HYT25844.1"
                     /translation="MNIPIDELRFLEREKGIALDTVVSALESALAAAYKRQPNAPDDA
                     RVVVDRNTGEVTVYAQELDEDERVVREWEDTPSDFGRIAAVTAKQVIVQRLREAERDV
                     TFGEYEGRVGDIVTGIVQQRDPRHVTVDLGKVEADLPHSEQVPSEHYQHNDRLKVYVT
                     DVVRSARGLRVVVSRTHPNLIKRLFELEVPEIAQG"
     gene            complement(643..1101)
                     /locus_tag="VEP73_05110"
     CDS             complement(643..1101)
                     /locus_tag="VEP73_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25845.1"
                     /translation="MATLPTRVLELAQRAAAGHGVEVLEARLRRQGRSQVVDVVLDAD
                     TLVEADVVEQVSKELSDLLDQEDPLPGSYLLEVTTPGLERPLHSARDFRRQLGHEVRV
                     TRTSSAGAAPGTVQGVVVAVDDEAVTLAAGDGQLRVPLPDVVRGKVVLPW"
     gene            complement(1290..>2072)
                     /locus_tag="VEP73_05115"
     CDS             complement(1290..>2072)
                     /locus_tag="VEP73_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25846.1"
                     /translation="LVDVLEHPALTRPELRSVLGRAGRGLAVLAARAGDAEGAEPPAG
                     PVAPTGPVPAAARTAGRLDERGWARVVRALAEHAASVVVDCGPGLAAPGTRAAVAAAD
                     QLVLVVDARLPRAAPAAAGPLAALGRPAAVVANQAPAGLRAGEVLERVPRARGAVLLP
                     AGAAGLGALPFGWESVPRAAWGQASRSAWRQAYELAALLVSDWPSLGLADGPVPGLAD
                     ARLEDAADPPARPRQPARSGRRLPRSPEPNRGTANAQARRLE"
BASE COUNT          258 a          805 c          743 g          266 t
ORIGIN      
        1 ccctgcgcga tctcgggcac ctcgagctcg aacagccgct tgatcaggtt cgggtgcgtg
       61 cgcgacacga ccacccgcag tccgcgggcc gagcggacca cgtcggtgac gtagaccttg
      121 aggcggtcgt tgtgctggta gtgctcgctc ggcacctgct cggagtgcgg caggtcggcc
      181 tccaccttgc cgaggtcgac cgtcacgtgg cgcgggtcgc gctgctgcac gatgccggtg
      241 acgatgtcgc cgacccggcc ctcgtactca ccgaaggtga cgtcgcgctc ggcctcgcgc
      301 agacgctgca cgatgacctg cttggcggtg acggcggcga tgcggccgaa gtccgacggc
      361 gtgtcctccc actcgcggac gacccgctcg tcctcgtcga gctcctgggc gtagacggtg
      421 acctcgccgg tgttgcggtc gaccaccacg cgggcgtcgt ccggcgcgtt cggctgccgc
      481 ttgtaggctg ccgccagcgc gctctcgagc gcgctgacca cggtgtcgag cgcgatgccc
      541 ttctcacgct cgagaaagcg cagctcgtcg atggggatgt tcacgtcgcc gtcctctcgt
      601 tgccggtgcc gaccggacgc tggccctgct gactggctgt cgtcaccacg gcaacaccac
      661 ctttccccgc accacgtcgg gcagcggcac ccggagctgg ccgtcgccgg cggcgagcgt
      721 caccgcctcg tcgtcgaccg ccaccacgac cccctggacg gtgcccggcg cggccccggc
      781 cgacgaggtc cgtgtcacgc ggacctcgtg gccgagctgg cgccggaagt cccgggcgga
      841 atgcagcggg cgctcgagcc cgggggtggt gacctcgagg aggtagctgc caggcagcgg
      901 gtcctcctgg tcgagaaggt cggacagctc cttggagacc tgctcgacga cgtcggcctc
      961 gaccagggtg tcggcgtcca gcacgacgtc gacgacctgc gaccggccct gccggcggag
     1021 ccgggcctcg agcacctcga caccgtgacc ggcggccgcc cgctgggcca gttcgaggac
     1081 acgcgtcggg agcgtcgcca cggtcgcgta cctccctcgc atgcttacgt tgctggtcgc
     1141 cctctcggta gcggacccac acaccggtct gctggcgggt ggcgagcccc ggacaaaatt
     1201 gaagtgggcc aacgcccact tcgttcgtta cacaggtgtc acccgccgcc gacacatgac
     1261 gatagcactc tcctggaatg cctagcaacc tactcgagcc ttctggcctg cgcatttgcc
     1321 gtgccgcggt tcggctcggg ggaccgtggc aggcgacgcc cgctgcgagc cggctggcgt
     1381 ggccgggccg gcgggtccgc cgcatcctcg aggcgggcgt ccgcgagccc ggggaccggc
     1441 ccgtccgcga gcccgaggga gggccagtcg gacacgagca gggcggcgag ctcgtacgcc
     1501 tgccgccacg cgctcctgga cgcctgcccc cacgcggccc tgggcacgct ctcccagccg
     1561 aacggcaagg caccgagccc cgccgcgccg gcgggcagca ggaccgcgcc ccgcgcccga
     1621 gggacccgct cgagcacctc gccggcccgg agacccgctg gcgcctggtt cgcgaccacc
     1681 gccgccggcc gcccgagcgc ggccagcggc ccggcggcgg ccggagccgc ccgcgggagc
     1741 cgggcgtcga ccacaagcac gagctggtcc gcggcggcga ccgcggcccg cgtccccggt
     1801 gcggcgagcc cgggcccaca gtccaccacg acgctcgccg catgctccgc gagcgcgcgc
     1861 acgacccgtg cccacccccg ctcgtcgagc cggccggcgg tccgggcggc ggcggggacc
     1921 ggcccggtgg gcgccaccgg cccggcgggc ggctccgcgc cctcggcgtc ccccgcacgg
     1981 gcggcgagga ccgccagtcc ccgaccggcc cggccgagca cgctccgcag ctccggacgg
     2041 gtgagcgctg ggtgctcgag cacgtcgacc ag
//
LOCUS       DASPYY010000333         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1742383, whole genome shotgun sequence.
ACCESSION   DASPYY010000333 DASPYY010000000
VERSION     DASPYY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742383"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1241
                     /gene="glpX"
                     /locus_tag="VEP73_05120"
     CDS             240..1241
                     /gene="glpX"
                     /locus_tag="VEP73_05120"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HYT25847.1"
                     /translation="MSARPGSEAPDRNLALELVRVTEAAAMAAARKMGYGDKELVDQA
                     AVDAMRAVLGSVPMTGVVVIGEGEKDNAPMLYNGEEIGNGLPPQVDIAVDPVDGTSLT
                     AKSMPNAIAVVALSEQGTMFDPGPCVYMEKLAVGRDGAGVVDFEAPIGANLLALAKAK
                     GGEVPDLTVCILDRPRHADVIREVREAGARIKLIPDGDVAGAIMAARDGTGVDMLVGV
                     GGTPEAVIAACALKCMDAAMFGRLWPRDDEERRAALDAGYDLDQVLTIDDLVRGDNVF
                     FAATGVTDGELLRGVRFDRRGATTQSLSMRSKSGTVRLIEARHQFSKLSRYATIDFG"
     gene            1248..>1564
                     /locus_tag="VEP73_05125"
     CDS             1248..>1564
                     /locus_tag="VEP73_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT25848.1"
                     /translation="MPSRQPVVVVGAGVSGLACARVLADSGLPVVVLERSAGVGGRMA
                     SRRVDGTVADHGAQYVTASDPGFAALLRRLAATGLAEPWATRLHRFDRGRLSSGGAAE
                     SGRV"
BASE COUNT          205 a          551 c          607 g          201 t
ORIGIN      
        1 gtccgggtgg gatcggaggc gccagggccc tgctgccgga ggccacgctc gcccgggcgg
       61 gcaggggcct tccaccacat cagggtaagc gagggcggcc gcgtgcgcca ggcctttgac
      121 ccagggcacg accgggacat cttgcgggac ctgtgccggg accccggggg gactccggca
      181 gctgttactg tcgaggcggc ggcccgagag ccgcgagcga ccgaccggag gtggtcccga
      241 tgagcgctcg tcccggcagc gaagcccccg accgcaacct ggccctcgag ctggtccgcg
      301 tgaccgaggc ggcggccatg gccgccgccc gcaagatggg ctacggcgac aaggagctgg
      361 tcgaccaggc agcggtcgac gccatgcggg cggtcctcgg ctcggtgccg atgaccggcg
      421 tggtggtcat cggcgagggc gagaaggaca acgcgccgat gctctacaac ggcgaggaga
      481 tcggcaacgg cctgcccccg caggtcgaca tcgcggtcga cccggtggac ggaaccagcc
      541 tgaccgcgaa gagcatgccg aacgcgatcg ccgtggtcgc cctgtccgag cagggcacga
      601 tgttcgaccc gggtccctgc gtctacatgg agaagctggc cgtcggccgg gacggcgccg
      661 gggtggtcga cttcgaggct cccatcggcg ccaacctcct ggcgctggcc aaggccaagg
      721 gtggcgaggt gcccgacctc accgtctgca tcctggaccg gccgcggcac gccgacgtca
      781 tccgcgaggt ccgcgaggcg ggcgcgcgca tcaagctgat cccggacggc gacgtcgccg
      841 gcgcgatcat ggccgcccgc gacggcacgg gcgtcgacat gctcgtcggc gtcgggggga
      901 cgcccgaggc cgtgatcgcc gcgtgcgcgc tcaagtgcat ggacgccgcc atgttcgggc
      961 ggctctggcc gcgcgacgac gaggagcggc gggcggcgct ggacgccggc tacgacctcg
     1021 accaggtcct caccatcgac gacctggtcc ggggcgacaa cgtgttcttc gcggcgaccg
     1081 gggtgaccga cggcgagctg ctgcggggtg tccgcttcga ccggcgtggg gcgacgaccc
     1141 agtcgctgtc gatgcgctcg aagtcgggca cggtgcggct gatcgaggcc cgccaccagt
     1201 tctcgaagct cagccgctac gcgaccatcg acttcgggta gccgcccatg ccgtcccgcc
     1261 agccggtcgt ggtcgtcggg gccggggtgt ccggcctggc gtgcgcgcgc gtgctcgccg
     1321 actccggcct gcccgtcgtc gtgctggagc gaagcgcggg tgtcggcggg cggatggcct
     1381 cccggcgggt ggacggcacg gtcgccgacc atggcgccca gtacgtgacc gcgagcgacc
     1441 ccgggttcgc ggcgctgctg cgccggctgg ccgccaccgg tctggccgag ccctgggcga
     1501 cccgcctgca ccgcttcgac cgtgggcggc tctccagtgg cggggcggcc gagtcgggcc
     1561 gggt
//
LOCUS       DASPYY010000334         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_758919, whole genome shotgun sequence.
ACCESSION   DASPYY010000334 DASPYY010000000
VERSION     DASPYY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758919"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VEP73_05130"
     CDS             <1..946
                     /locus_tag="VEP73_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25849.1"
                     /translation="GAKGLRRLQQAAVPTLAISGNHDTPRLRESGSPYAVLADAFPGL
                     RFAYRGACEAIDLLPGLRVHAVPQCSDDQVLKDQIAEAAAARAADHHNLMVTHAAVTV
                     LSRRYTYGDVNELELDLSTLDAGFDRILLGHFHNFAQVAPNAWYPGSTDTFSFKDLPS
                     GDEPEVKGILSLDTQNGRVRHHPVPGGRPLRTYKIDACNLDLGELYDAVATLVQDPSE
                     TTGAVVRLFVNQARPEVRRLLDRRTVAESFPGALAVTVHVEAADEEGAAQLAAQPVAS
                     LADEWDRYLANVPIEGYDRDRLATLGRTYLQHAEEGAR"
     gene            946..3336
                     /locus_tag="VEP73_05135"
     CDS             946..3336
                     /locus_tag="VEP73_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HYT25850.1"
                     /translation="MHLRSLTLRNYRVYKAVDLEFPDGLIGIYGSNGAGKTTLIESVR
                     YALYGDARTDKWELRSAGVGEDVRVELVFEHEGNSYEVRRRLKGRNLTPDVEVFCNGQ
                     LAVQSVREANAYLGRVVGMDQRAFLASVCAQQKELTAFATMMPAERRKLVLDLLGVSP
                     IEKALAKVREEARDAKTAAGAARAQLPDLGAAEQAAEEAATAAEEAVTAAGSAAAVAA
                     AAQQALEGAQAALSEAEGVARRVEELRQQANLARAEAAGHRREAERHEQRAQQADALG
                     PEVAAAEARVVELEAAAAPLEALEAAREVEVHRNALLRALRDAQSRAETAQRAHHEAE
                     QQATAGGGVVAARVAAEEAAEALEQELAAARERHGAASEALGAATARLQAAQAAVERG
                     ATLDPDAPCPTCGQPLGPAHAEVTRAHAAELADARAAHAKATAERAAVVQAGKALAAR
                     RETLQQDLKAAREAEQRAARAVIRAEEAERAMRVAAEEVAARTAELAGAPDPGFDPAA
                     YAVAKQAAGALQQARLDLGALRGRAQRADAERALAKEALARADQADGQAAKLDTELAT
                     PALAGAEQARAAARAATDAAAQAARHTHGEQQRSAEALAGARRLAEQRTTALTAARAR
                     HDEVAGLEEQSAYLARLVDLVTGFRLHLVSRLGRRLSVEAAGLFAELTDHEYQDLVVD
                     PEDYSVRIADNGAEYELARFSGSENDLASLSLRVAVSLVIAEGAGELGLLVLDEVLGS
                     LDRERRERMLSALTTLQGRFRQVLLVTHNDEVKDMLPAAIEVRKGPHRTSMATVLA"
BASE COUNT          539 a         1353 c         1234 g          414 t
ORIGIN      
        1 gggcgccaag ggcctgcgcc gcctgcagca ggcagccgtc ccgaccctgg cgatctccgg
       61 caaccacgac accccccgcc tgcgcgagtc cggctcgccc tacgcggtcc tcgccgacgc
      121 cttccccggc ctccgcttcg cctaccgcgg cgcctgcgag gccatcgacc tgctgccggg
      181 actccgcgtc cacgcggtcc cgcaatgcag cgacgaccag gtcctcaaag accagatcgc
      241 cgaggccgcg gcagcccgcg ccgccgacca ccacaacctg atggtcaccc acgcggccgt
      301 cacggtgctg tcgcgccgct acacctacgg cgacgtcaac gagctggagc tcgacctgtc
      361 caccctcgac gccggcttcg accgcatcct gctcggccac ttccacaact tcgcgcaggt
      421 cgcccccaac gcctggtatc ccggctccac cgacaccttc agctttaaag acctcccctc
      481 cggcgacgag ccggaggtca agggcatcct gtcgctggac acccagaacg gccgcgtccg
      541 ccaccacccg gttcctggtg gccggccctt gcggacctac aagatcgacg cctgcaacct
      601 cgacctcggc gagctctacg acgccgtcgc cacgctggtg caggacccgt ccgaaaccac
      661 cggcgcggtc gtccgcctgt tcgtcaacca ggcccgcccg gaggtgcgcc ggctgctgga
      721 ccgccgcacc gtcgccgagt ccttccccgg cgccctggcc gtcaccgtcc acgtcgaagc
      781 cgccgacgaa gagggcgccg cccagctcgc cgcccaaccc gtcgccagcc tcgccgacga
      841 atgggaccgc tacctcgcca acgtcccgat cgaaggctac gaccgcgacc gcctcgccac
      901 cctcggccgc acctacctgc aacacgccga ggagggggcg cgctgatgca cctgcgctcc
      961 ctcaccctgc gcaactaccg ggtctacaag gccgttgacc tggagtttcc tgatgggtta
     1021 attggtattt atggttcgaa cggggcgggc aagacgaccc tcatcgaaag tgtccgctat
     1081 gccctctacg gcgacgcccg gaccgacaag tgggaattgc ggtcggctgg ggtgggggag
     1141 gacgtgcggg tcgagctggt cttcgagcat gagggcaaca gctatgaggt gcggcggcgg
     1201 ctcaagggcc gaaacctcac gcccgacgtg gaggtgttct gcaacgggca gctggcggtg
     1261 cagtcggtca gggaggccaa cgcctacctc gggcgggtgg ttgggatgga ccagcgggcg
     1321 tttctggcgt cggtgtgcgc gcagcagaag gagctgaccg cgttcgcgac catgatgccg
     1381 gcggagcggc gcaagctggt gctggacctg cttggggtca gcccgatcga gaaggcgctc
     1441 gccaaggtgc gggaggaggc ccgggacgcc aagaccgccg ccggggctgc gcgggcgcag
     1501 ctgcccgacc tgggcgcggc cgagcaggcc gccgaggagg ccgccacggc cgccgaggag
     1561 gccgttaccg ccgccgggag cgccgccgcc gtggccgctg ccgcgcagca ggcgctggaa
     1621 ggcgcccagg ccgcgctcag cgaggcggag ggcgtcgccc gtcgggtcga ggagctgcgg
     1681 cagcaggcca acctcgcccg ggcggaggcc gcagggcatc ggcgggaggc ggagcggcat
     1741 gagcagcgtg cccagcaggc cgacgccctc ggcccggaag tcgccgctgc cgaggcacgt
     1801 gttgtcgagc tggaggcggc ggccgccccc ctggaagccc tggaggccgc ccgcgaggtg
     1861 gaagtgcacc gcaacgcgct gctgcgggcg ctccgcgacg cccagagccg ggcggagacc
     1921 gcccagcgcg cccaccacga agccgagcag caggccactg ccggcggggg agtggtagcg
     1981 gcccgggtcg ccgccgagga ggcggctgag gcgctggaac aggagctggc agcggccagg
     2041 gagcgccacg gcgccgccag cgaggcgctc ggcgcggcca ccgcgcggct acaggccgcg
     2101 caggcggcgg tggaacgcgg cgccaccctg gaccccgacg cgccctgccc gacctgcggg
     2161 cagccgctcg gccctgcgca tgccgaggtc acccgcgccc acgccgccga gctggctgac
     2221 gcccgtgccg cccatgcaaa ggccaccgcc gagcgggccg ccgtggtcca ggccggcaag
     2281 gcgctggccg cccggcgtga gaccctgcag caggacctca aggcggcgcg ggaggccgaa
     2341 cagcgcgccg cccgcgcggt catccgcgcc gaggaggccg agcgggccat gcgggtcgcc
     2401 gccgaggagg tcgccgcccg caccgcggag ctcgccgggg ctcccgatcc cggcttcgac
     2461 ccggccgcct acgccgtagc caagcaggcc gccggggcgc tgcagcaggc ccgcctggac
     2521 ctcggcgcgc tgcgcggccg cgcgcagcgc gccgacgccg agcgggccct cgccaaggag
     2581 gcactcgccc gcgccgacca ggccgacggc caggccgcca aactcgacac cgaactcgcc
     2641 acccccgccc tcgccggcgc cgagcaggcc cgcgccgccg cccgcgccgc caccgacgcg
     2701 gccgcccagg ccgcccgcca cacccacggc gagcagcagc gcagcgccga ggcactggcc
     2761 ggcgcccgcc gcctcgccga gcagcgcacc accgcgctga ccgccgcccg tgcccgccac
     2821 gacgaggtcg caggcctcga ggagcaatcc gcctacctcg cccgactggt cgacctggtc
     2881 accgggttcc gcctccatct ggtgtcgcgg ctggggcgcc gcctgtcggt cgaggcggcc
     2941 gggctgttcg ccgagctgac cgaccacgag taccaggacc tggtggtcga cccggaggat
     3001 tactcggtac gcatcgccga caacggcgcc gaatatgaac tggcacgctt ctccggttcc
     3061 gagaacgacc tcgccagcct gtcgttgcgg gtggcggtca gcctggtgat cgccgagggt
     3121 gcgggtgagc tcggtctgct ggtgctcgac gaggtgctcg gctcactgga ccgtgagcgc
     3181 cgcgagcgta tgctgtccgc cctcacgacc ctgcagggcc gcttccgcca ggtcctgctg
     3241 gtgacccaca acgacgaggt gaaggacatg cttcccgcgg cgatcgaggt ccgcaagggc
     3301 ccacaccgga cgtcgatggc gacggtgctg gcctagaccc ggcctagacc ctttgcgcgt
     3361 tcggaagttc gcatttagcg tgcggcattc gtcgggaaca accacgctct gccgggtgtt
     3421 acgcacacag caccggcacg cagtcacgca atacgccagt tcgtgataca taagccggcg
     3481 caccacgctc cctacatcac gaacggaatg aaaggaacta agggtgcctg aggacgaaga
//
LOCUS       DASPYY010000335         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_337746, whole genome shotgun sequence.
ACCESSION   DASPYY010000335 DASPYY010000000
VERSION     DASPYY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337746"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="VEP73_05140"
     CDS             <1..1706
                     /locus_tag="VEP73_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYT25851.1"
                     /translation="PAGGAVPAAAVSTAPASQAPAPPEPEAPAAAAPPAPGRAGGRSG
                     RQRGASAATGAPAAATTPEVGTAARPERPGRVRGRGGLAAPGRGRAEAQAAAPATAEP
                     DARAAPAEPATGGSTRLDARGRGGQGSRQADRGRRRRASGLTEEQKRVLREEGPEKRM
                     LVTEGAERTQIGVLEGRTLVEHYVTRKAGRSLVGNVYLGRVQNVLPGMEAAFVDIGKG
                     RNAVLYAGEVNYNEEDLDGEAPRIEKALKPGQSVLVQVTKDPIGAKGARLTAQVSLAG
                     RYLVLQPEDNTFGISRRLPEDERSRLREILKEVRPKGLGLIVRTAAEGATADDLRADL
                     ARLQARWEAVGGKARKASAPAVIYSEPELVVRVIRDIFSPDFVELAVDAPELYERVKD
                     YLEEVAPDLLPRLRLHDGRLPLFEERHVVEQIHKALERKVWLPSGGSIVIDRTEAMTV
                     VDVNTGKFVGKSNLEETVVANNLEAADEIVRQLRLRDIGGIIIIDFIDMLFERNQQAV
                     VERLRAALARDKTKSQVMEVSSLGLVQMTRKRVSGGLLESFSETCPTCEGRGVVITHE
                     T"
BASE COUNT          242 a          666 c          655 g          190 t
ORIGIN      
        1 cccctgccgg cggggcggtg cccgcggcgg cggtgtccac cgcgcccgcc agccaggcgc
       61 ccgcgcctcc cgagccagag gctccggcgg ccgccgcgcc gccggccccc ggccgcgccg
      121 gcgggcgctc cggccgccag cgcggagcgt ccgccgccac cggcgcaccc gcggccgcca
      181 cgacccccga ggtcggcacg gccgccaggc ccgagcggcc gggccgcgtc cggggccggg
      241 gcgggctcgc ggcgcccggg cgcggccggg ccgaggcgca ggcggcggcc ccggcgaccg
      301 ccgagcccga cgcccgtgcc gcgccggccg aacccgcgac gggcggctcg acccgcctgg
      361 acgcgcgcgg ccggggtggg cagggctccc gccaggccga ccggggccgc cggcggcgcg
      421 cctccggcct caccgaggag cagaagcggg tcctccgcga ggagggcccg gagaagcgga
      481 tgctggtcac cgagggggcc gagcgcaccc agatcggcgt gctcgagggc cgcaccctgg
      541 tcgagcacta cgtgacccgc aaggcgggcc gctcgctcgt cggcaacgtc tacctcggcc
      601 gggtccagaa cgtcctgccc ggcatggagg ccgccttcgt cgacatcggc aagggccgca
      661 acgcggtcct gtacgccggc gaggtcaact acaacgagga ggacctggac ggcgaggcgc
      721 cccgcatcga gaaggcgctg aagcccggcc agtcggtcct cgtccaggtc accaaggacc
      781 ccatcggggc caagggcgcc cgcctgaccg cgcaggtctc cctggccggc cgctacctcg
      841 tgctgcagcc ggaggacaac acgttcggga tctcccggcg gctgcccgag gacgagcgct
      901 cgcgcctgcg cgagatcctc aaggaggtgc ggccgaaggg cctcgggctg atcgtccgca
      961 ccgccgccga gggcgccacg gccgacgacc tgcgcgccga cctcgcccgc ctgcaggcgc
     1021 gctgggaggc ggtcgggggc aaggcgagga aggcgtccgc cccggcggtc atctactccg
     1081 agcccgagct ggtcgtccgg gtgatccgcg acatcttctc gcccgacttc gtcgagctgg
     1141 cggtcgacgc ccccgagctg tacgagcggg tcaaggacta cctcgaggag gtcgccccgg
     1201 acctgctgcc caggctgcgc ctgcacgacg gcaggctgcc gctgttcgag gagcgccatg
     1261 tcgtcgagca gatccacaag gcgctcgagc gcaaggtctg gctgccgtcg ggcgggtcga
     1321 tcgtgatcga ccggaccgag gcgatgaccg tggtcgacgt gaacacgggc aagttcgtcg
     1381 gcaagtcgaa cctcgaggag accgtggtcg cgaacaacct ggaggcggcc gacgagatcg
     1441 tgcgccagct ccggctccgc gacatcggcg gcatcatcat catcgacttc atcgacatgc
     1501 tgttcgagcg caaccagcag gcggtcgtcg agcggctgcg ggccgcgctg gccagggaca
     1561 agacgaagag ccaggtgatg gaggtctcct cgctcggcct cgtccagatg acccgcaagc
     1621 gcgtctccgg cggcctgctc gagagcttct cggagacctg cccgacctgc gaggggcgcg
     1681 gtgtcgtcat cacccacgag acctgacctg cctgcccggg gacggcggcg ccccgcccct
     1741 cgcaggtcgg gca
//
LOCUS       DASPYY010000336         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1463416, whole genome shotgun sequence.
ACCESSION   DASPYY010000336 DASPYY010000000
VERSION     DASPYY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463416"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..825
                     /locus_tag="VEP73_05145"
     CDS             58..825
                     /locus_tag="VEP73_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25852.1"
                     /translation="MARTFLAILWRDVFVTGRELPAFLAQVVLQPFFLIFIFGRVLAD
                     LGFTRPGYAQLLLPGVIALTVVLTALQGTALPLVIEFSFTREIEDRLLAPLPVSMVAV
                     EKVVFAAVRSLIAGAVMFPIGALVFGHLSFRADRLWLVVLFGLLGALAGGGMGLTLGT
                     LVPPNRINIMFALVLTPLLFTGCTQYPWPSLSKLPWFKVFTLVNPLTYVSEGMRSAMV
                     PDLAPHMPTAVAAGLLALAAAVFAVTGIRGFLRRAID"
     gene            933..1310
                     /locus_tag="VEP73_05150"
     CDS             933..1310
                     /locus_tag="VEP73_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25853.1"
                     /translation="MPVRRRFTRRFEEMPSVRRFVSSALLRDTEAYEAARLLVDEAVT
                     NPLLHSAACDADRTFQVSMTFAPNLLHVEVHDAGVPFPLHRNGQPIDSVAGRSLELFD
                     ELAAGWDSTRDATGHLVWFEVEV"
     gene            complement(1345..>2149)
                     /locus_tag="VEP73_05155"
     CDS             complement(1345..>2149)
                     /locus_tag="VEP73_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYT25854.1"
                     /translation="VDDLAYPLPVTVICELFGVPPADRARFRAWSGALVHLLDPLVAT
                     DVLERALEARSALRGYLHELVADRRAHPGSDLLTALIAAEAEGQRLSEAELISMCVLL
                     LIAGHETTVNLISNGTLALLRHPVELARLAADPSLAPSAVEELLRYDSPVQLTSRHAL
                     ADVEIGGRLVREGETVIAGLGAANRDPAAFADPDQLDLGRAPNRHVAFGGGIHFCLGA
                     PLARMEARIALTALLGRVPGLALGNGEPALRDTVTLRGLASLPVRFGAG"
BASE COUNT          257 a          791 c          787 g          314 t
ORIGIN      
        1 gacgtcgcca ccaccccggc gccccccgcc gccgggcccg ccggcacacg gtccgggatg
       61 gcgcgcacgt tcctcgccat cctctggcgc gacgtgttcg tcaccggccg cgagctgccc
      121 gccttcctgg cccaggtggt gctgcagccg ttcttcctca tcttcatctt cggccgggtg
      181 ctcgccgacc tcgggttcac ccggcccggc tacgcgcagc tcctgctgcc cggcgtgatc
      241 gcgctcaccg tggtgctcac cgccctgcag ggcaccgccc tgccgctcgt gatcgagttc
      301 agcttcacca gggagatcga ggaccggctg ctcgcgccgc tgccggtgag catggtggcg
      361 gtcgagaagg tcgtgttcgc cgcggtccgg tcgctgatcg ccggggcggt catgttcccg
      421 atcggggcgc tcgtgttcgg ccacctctcc ttccgcgccg accggctctg gctcgtcgtg
      481 ctgttcggcc tgctcggggc gctcgcgggc ggcggcatgg ggctgaccct cggcacgctc
      541 gtgccgccga accggatcaa catcatgttc gccctcgtgc tgacgccgct gctgttcacc
      601 gggtgcaccc agtacccgtg gccctcgctc tccaagctgc cgtggttcaa ggtcttcacc
      661 ctggtgaacc cgctcaccta cgtgagcgag ggcatgcgct cggcgatggt ccccgacctc
      721 gctccccaca tgccgaccgc ggtcgccgcc gggctgctcg cgctcgcggc ggccgtgttc
      781 gcggtcaccg gcatccgcgg cttcctccgg cgcgcgatcg actgacgccg tccccgtccc
      841 ttctgcacgg agcaggattc gggcccggcg accgtgggta gcgacggcgt gtagcggccc
      901 cgccgcccgg tcccgacggg aggaggacga gcttgccagt acggcgacgg ttcaccagac
      961 ggttcgagga gatgccgtcc gtgcggcggt tcgtctcgag cgcgctgctg cgcgacaccg
     1021 aggcgtacga ggcggcccgc ctccttgtgg acgaggcggt caccaacccg ctgctgcact
     1081 cggccgcctg cgacgccgac cgcaccttcc aggtgagcat gacgttcgcg ccgaacctgc
     1141 tccacgtcga ggtgcacgac gcgggcgtgc ccttccccct gcacaggaac ggccagccga
     1201 tcgactcggt cgctgggcgg agcctcgagc tgttcgacga gctcgccgcg ggctgggact
     1261 ccacccgaga cgccaccggc cacctggtct ggttcgaggt ggaggtctga ccggctcagc
     1321 cggcgaggtg gaggtctgac cggctcagcc ggctccgaag cgcacgggca ggctcgcgag
     1381 gccccgcagg gtgacggtgt cccgcagggc cggctccccg ttgccgaggg cgaggccggg
     1441 cacgcggccg aggagcgcgg tgagcgcgat ccgcgcctcc atgcgggcga gcggggcgcc
     1501 gaggcagaag tggatcccgc ctccgaaggc gacgtgccgg ttcggcgcgc gcccgaggtc
     1561 gagctggtcc gggtcggcga acgcggccgg gtcccggttc gccgcgccca ggccggcgat
     1621 gacggtctcg ccctcgcgga ccagccggcc gccgatctcg acgtcggcga gcgcgtgcct
     1681 cgacgtcagc tgcaccgggc tgtcgtaacg gagcagctcc tcgaccgcgc tcggggcgag
     1741 cgacgggtcg gcggcgagcc gcgccagctc gacggggtgg cgcagcagcg cgagggtgcc
     1801 gttcgagatg aggttgacgg tcgtctcgtg cccggcgatg agcagcagga cgcacatcga
     1861 gatgagctcg gcctcgctga ggcgctgccc ctcggcctcc gcggcgatga gcgcggtgag
     1921 caggtcgctg ccggggtgcg cgcgccggtc ggcgaccagc tcgtgcaggt agccgcgcag
     1981 cgccgagcgc gcctcgagcg cccgctcgag gacgtcggtg gcgacgagcg ggtcgagcag
     2041 gtggacgagc gcgcccgacc atgcgcggaa ccgcgcacgg tcggcgggcg ggaccccgaa
     2101 cagctcgcag atgaccgtca ccggcagcgg gtaggcgaga tcgtcgacg
//
LOCUS       DASPYY010000337         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2305609, whole genome shotgun sequence.
ACCESSION   DASPYY010000337 DASPYY010000000
VERSION     DASPYY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305609"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VEP73_05160"
     CDS             <1..805
                     /locus_tag="VEP73_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT25855.1"
                     /translation="ATGRGAPADGREADLDAGGWAAGWAAADALARSVVDGLLDGWDE
                     PFEGRVARDLVAALPDGATVVVGSSMPVRDVDAFARPREGLGFVANRGASGIDGFVAT
                     ALGVAAAGGGPVAALCGDLTFLHDASGVLEARRRPGPVVFVVCDNDGGGIFSFLPQAE
                     LPERWFEPLFGTPHGLDLGAVAAAAGLPWQRVARAGDLVGAVAAALASGRTSVLLVRT
                     DRAANAARHREVTRAVVEALEGRADPAAGVVPDGPGLHRGAGGRDLGSR"
     gene            complement(818..>2015)
                     /locus_tag="VEP73_05165"
     CDS             complement(818..>2015)
                     /locus_tag="VEP73_05165"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HYT25856.1"
                     /translation="GEVATAGVAAEVRVPTGRDRVQRAADATGALLGQARVDDPEGTG
                     LGPLAAGAVPFGEATPGRLSVPALVVRRGPDRALWAVATAAGRRPDPPDLRTRPRAGA
                     GGDGAWPEVRAVRAPAGQDRAGFQAAVRAALDVIAGPRLGGGRLGGGLLGGGGLLDKV
                     VLARELLVEAAGPFPRQAVLRRLRERAAGSFVYASGGFVGASPELLVARRGRTATSRP
                     MAGTVPRGGAAGEEAGREAWLRASGKETAEHRLVVDMVAETLAKVADDIAVAPVEVVR
                     LPTVAHLATRVSAELTAPVPSALELAGLLHPTPAVAGSPRDAALEVLAGLEPFDRGRY
                     GGPVGWVDHRGDGEWAVALRCATLHRAGARLVAGAGIVAGSDPDAEWAETGYKLEAML
                     RVLTSS"
BASE COUNT          201 a          815 c          806 g          193 t
ORIGIN      
        1 agcgaccggc cggggcgcgc cggcggacgg ccgcgaggcg gacctcgacg cgggaggctg
       61 ggccgccggg tgggccgccg ccgacgcgct cgcccggtcg gtcgtggacg ggctgctcga
      121 cggctgggac gagccgttcg agggccgcgt cgcccgcgac ctggtcgccg cgctccccga
      181 cggggcgacg gtggtggtcg ggtccagcat gcccgtgcgc gacgtggacg ccttcgcgag
      241 gccccgcgag ggcctggggt tcgtggccaa ccgcggcgcg agcggcatcg acgggttcgt
      301 cgcgaccgcc ctcggggtgg ccgcggccgg cggcggcccg gtcgccgcgc tctgcggcga
      361 cctgaccttc ctccacgacg cgagcggcgt cctcgaggcc cgccgccggc cagggccggt
      421 cgtcttcgtc gtctgcgaca acgacggcgg cgggatcttc tcgttcctgc cccaggccga
      481 gctgcctgag cgctggttcg agccgctgtt cggcaccccg cacggcctcg acctcggcgc
      541 cgtggccgcc gccgccggcc tgccctggca gcgggtggcc cgggccggcg acctcgtcgg
      601 ggccgtcgcc gcggcgctcg cgagcggccg gacgagcgtg ctgctcgtcc gcaccgaccg
      661 cgccgccaac gcggccaggc acagggaggt gacccgcgcg gtggtggagg cgctcgaagg
      721 gcgggccgac ccggccgcgg gggtcgtccc ggacgggcct ggcctccacc ggggagcggg
      781 cgggcgggac ctcgggagcc ggtgagcaac cgcagcatca ggagctggtg agcacccgga
      841 gcatcgcctc gagcttgtag ccggtctcgg cccactcggc gtccgggtcc gagccggcga
      901 cgatcccggc acccgccacc agccgggcgc ccgcgcggtg gagggtcgcg cagcgcagcg
      961 cgaccgccca ctccccgtcg ccacggtggt cgacccagcc gaccggcccg ccgtagcgac
     1021 cgcggtcgaa cggctccagg ccggcgagca cctcgagggc cgcgtcgcgg ggcgagcccg
     1081 cgacggctgg cgtcgggtgc aggaggccgg ccagctcgag cgctgacggc accggcgcgg
     1141 tcagctcggc cgagacccgg gtcgcgaggt gcgcgacggt cggcaggcgc accacctcga
     1201 ccggggcgac ggcgatgtcg tcggccacct tggccagcgt ctcggccacc atgtcgacca
     1261 ccaggcggtg ctcggcggtc tccttgcccg aggcgcgcag ccacgcctcc cggccggcct
     1321 cctcgccggc cgccccgccg cgggggaccg tcccggccat cggccgggag gtcgccgtcc
     1381 tcccccgcct ggccacgagc agctccgggc tcgcgccgac gaacccgccg ctcgcgtaca
     1441 cgaacgagcc ggccgcgcgc tcgcggaggc ggcggagcac ggcctggcgc gggaagggtc
     1501 cggccgcctc gacgaggagc tcgcgggcga gcacgacctt gtccagcagg ccgccgccgc
     1561 ccagcaggcc gccgcccagc cgcccgccgc ccagccgagg gccggcgatc acgtcgaggg
     1621 cggcgcgcac cgccgcctgg aagccggccc ggtcctgccc ggcgggggcg cggaccgcgc
     1681 gcacctcggg ccaggcgccg tccccgcccg cgcctgcccg cggccgcgtg cgcaggtcgg
     1741 gcgggtcggg gcgccggccg gcggcggtcg ccaccgccca cagggcgcgg tcggggccgc
     1801 gccgcacgac cagcgccggc acgctgagcc gccctggggt ggcctcgccg aacgggaccg
     1861 cgccggccgc gagcgggccg aggccggtgc cctccggatc gtcgacccgg gcctggccga
     1921 gcagcgctcc ggtggcgtcg gcggcacgct gcacccggtc ccggccggtc gggacccgga
     1981 cctcggccgc gaccccggcg gtggccacct cgccg
//
LOCUS       DASPYY010000338         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2305816, whole genome shotgun sequence.
ACCESSION   DASPYY010000338 DASPYY010000000
VERSION     DASPYY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305816"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VEP73_05170"
     CDS             <1..447
                     /locus_tag="VEP73_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018585666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT25857.1"
                     /translation="NEVAVSRPSDALAARAAANTTFTGLLLGLGAVALLVGGIGVANT
                     MVISVLERRAEIGLRRSLGATRGHIRIQFLAESLLLSALGGGAGILLGSAVTAAHATL
                     QNWPTVVPAWVMAGGITAALIIGAGAGLYPAIRAARLAPTEALAVA"
     gene            complement(612..1304)
                     /locus_tag="VEP73_05175"
     CDS             complement(612..1304)
                     /locus_tag="VEP73_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT25858.1"
                     /translation="MTRVLIADDDHLMRAGLVELLTADPTIEIVGQAATGREAVQQAR
                     RLAPDVVLMDVRMPDLDGIAATRELSQAAPHVKVLILTTFEQDNYIFGALRAGASGFL
                     LKRTRPEDLIAAVHTIAAGDSLLSPSVTRRVVDRMAQQPTPDLADNARLAALTPRERE
                     TLALVARGLSNREIAAALSVEESTIRTHVKRILMKLDLRDRVQAVIFAYEAGMNQPGG
                     RRQVNTSADQAT"
     gene            complement(1301..>2427)
                     /locus_tag="VEP73_05180"
     CDS             complement(1301..>2427)
                     /locus_tag="VEP73_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYT25859.1"
                     /translation="AVALAGTLALVRHAGVAPTGPGSAGLDGTGVALIGCSTLPLIAW
                     RRYPLGLFAVTASASVALAGLRYPVDLILGPTVALYLLVASRGRETPWTRRVTAAVVG
                     LFVAYLAATAAAHAGFPGIELLHTGLAWAVAWFAGERTRLRREQVNELNQRALRAERD
                     AERERRLAVAEERARIARDLHDSAGHAISLIAVRAGAARLRHRQEPDRSLLALQAVED
                     LARHTADEIDQIVTTLREANPAGGPVEAPPGLASLDTLIAHHTAAGLDLTVDTVGEPR
                     PLGGAADQAAYRILQQALSNAARHGAGSARIQLAYADTAVALTVTNPVSPDAAPRPGG
                     GHGLIGMRERASLLGGSLDIERANGTFRVRALIPYPGQRP"
BASE COUNT          295 a          833 c          946 g          353 t
ORIGIN      
        1 aacgaggtgg cggtgtcccg cccctccgat gcgctcgccg cccgggccgc cgcgaacacc
       61 accttcaccg gcctgttgct cggcctaggc gccgtcgccc tgctcgtcgg cggcatcgga
      121 gtcgccaaca ccatggtgat ctccgtcctg gaacggcgcg ccgaaatcgg gctgcgccgc
      181 tccctgggcg ccacccgagg acacatccgc atccagttcc tcgccgagtc cctcctgctg
      241 tccgccctcg gcggcggcgc agggatcctg ctcggatcgg ctgtgacggc ggcgcacgcg
      301 acactgcaga actggcccac cgtagtcccc gcctgggtca tggccggagg catcaccgct
      361 gccctgatta tcggcgcggg ggccggcctc taccccgcca ttcgcgcggc caggctcgcc
      421 cccaccgagg cactcgccgt cgcctgactg acaggccagt ccgcctttgt ggtcatgtga
      481 ctggtcgcgg agcaccggcg gtggtgatgc ccacccggcg cagtgcgccg gtgggcatat
      541 acggtcgcag cgacgcgcgc aagtgtcgaa gccgacattc tggtcgatca caaggttcgc
      601 ggaccggcgt gtcacgtcgc ctggtcggcg gatgtgttca cctggcgccg gccgccgggt
      661 tggttcatgc cggcctcgta ggcgaagatc acggcttgga cccggtcgcg gaggtcgagc
      721 ttcatcagga tccgcttgac gtgcgttctg atcgtggact cctcgacgga gagcgcggcg
      781 gcgatctcgc ggttcgacag accccgggcg acgagggcga gggtctcgcg ttcgcggggg
      841 gtgagcgcag caagccgggc gttgtcggcg aggtccgggg tgggttgctg ggccatccgg
      901 tcgacgaccc ggcgggtgac cgagggcgac agcaggctgt cgccggcggc gatggtgtgc
      961 accgcggcga tgaggtcttc gggtcgggtg cgtttgagca ggaagccgga ggcgccggcg
     1021 cgcagcgcgc cgaagatgta gttgtcctgc tcgaacgtgg tcaggatcag gaccttcacg
     1081 tgtggagcgg cctgtgacag ctcgcgggtg gcggcgattc cgtcgaggtc gggcatgcgc
     1141 acgtccatca gcacgacgtc cggggcgagc cgccgcgctt gttgcaccgc ctcgcgtccg
     1201 gtcgccgcct gcccgacgat ctcgatggtg gggtctgcgg tgagcagctc gacgagcccg
     1261 gcgcgcatca ggtggtcgtc gtcggcgatc agtacccggg tcacgggcgc tgccctgggt
     1321 aggggatcag agcgcgaacc cggaaggtgc cgttggcgcg ctcgatgtcc aggctgccgc
     1381 cgagcaggct cgcgcgttcc cgcatgccga tcagcccgtg cccgccgccc ggacgcggtg
     1441 cggcgtcggg gcttaccggg ttggtgacgg tgagcgcgac cgccgtgtcg gcgtaggcga
     1501 gctggatgcg ggcgctgccg gctccgtggc gggccgcgtt cgacagcgcc tgctggagta
     1561 tccggtaggc ggcctggtcg gcggcgccgc ccagtgggcg cggctcgccg acggtgtcga
     1621 cggtcaggtc gagcccggcg gccgtgtggt gggcgatcag ggtgtccagc gacgccaggc
     1681 cgggcggggc ctcgaccggc ccgccggccg ggttggcctc gcgcagcgtg gtgacgatct
     1741 ggtcgatctc gtcggcggtg tgccgggcca ggtcctcgac cgcttgcagg gccagcagcg
     1801 agcggtctgg ctcctggcgg tgtcgcagcc gggcggcgcc ggcgcgcacg gcgatcaggc
     1861 tgatcgcgtg accggcggag tcgtgcaggt cgcgggcgat gcgggcgcgt tcctcggcga
     1921 cggcgagccg gcgctcgcgc tcggcgtccc gctcggcgcg cagtgcgcgc tggttgagct
     1981 cgttgacctg ctcgcgccgc agccgggtgc gttcgccggc gaaccaggcc actgcccagg
     2041 ccagaccggt gtgcagcagc tcgatgccgg gaaagccggc gtgcgcggcg gcggtcgcgg
     2101 ccaggtacgc gacgaacagc cccaccacgg cggcggtgac gcgcctcgtc cacggggtct
     2161 cccgcccgcg gctggcgacg agcaggtaca gcgcgaccgt ggggcccagg atcaggtcga
     2221 ccgggtagcg cagcccggcc agggccacgc tggccgacgc ggtcaccgcg aacaagccga
     2281 gcgggtagcg ccgccaggcg atcagtggca gcgtcgagca gccgatgagc gcgacgccgg
     2341 tcccgtcgag cccggcggat ccggggccgg tcggggcgac cccggcgtgg cgcaccagcg
     2401 ccagcgtgcc ggcgagtgcg acggccg
//
LOCUS       DASPYY010000339         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2164642, whole genome shotgun sequence.
ACCESSION   DASPYY010000339 DASPYY010000000
VERSION     DASPYY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164642"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1526)
                     /locus_tag="VEP73_05185"
     CDS             complement(135..1526)
                     /locus_tag="VEP73_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25860.1"
                     /translation="MAWLVRSGLVRVAAVVAACGVAVAVLVSPAQVGPARSEVAGHEQ
                     VNLWQFADQVVSRPDPGNPNTWDWTPAISAAISYCESVASWRYPVGGSGSYRVLGLPT
                     LYIPRGIYRNTASHPIEFVQGFTVRGDGPGATVIQHEGANFLFVVHRSGGLTFSDFTV
                     QGVSPAGPVGGLRGLVEGSGGFLFRESSGDGAPTGGSTWMNDVAGVEINELHRAFRFE
                     GDEMTDSLVVERFHGRDNFIDFDYANSQAVNHQLVGGEVTYGVSFPDAAYAARLGTWS
                     SHPALEDGAVFNVTSGGQVTVAGGSFIVRKPTLYFHVPPRDGSQGATSNVVGWSFTGT
                     RWEVRRPATSRDRFGLERVTMIRYANPFPTAAWVQPSVRFIGCDWNVMTPSIDLFYIA
                     NAVRVYAEMSRVFTPSASYRARLVSLLNGSTPAIRGTYRSWGTTPMPRARRAAPGYTP
                     PSTLDQQIAMPAA"
     gene            complement(1748..>3006)
                     /locus_tag="VEP73_05190"
     CDS             complement(1748..>3006)
                     /locus_tag="VEP73_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25861.1"
                     /translation="NYPGLPFEQRIPGTLFQVLDSDGRPIAGSTRPEAIAGLAVVIPV
                     ARGEAGSRYASVRLEGVPVRTITAPVNVIGFGRIGAVTLGLIDNTRATVGRVQRLLVG
                     SGLLALALAAVLAWVVGRAAMRPVTAISAAARAVADSGDPSKRVATGTHDEVGDLART
                     LNRMLARLEVAQAELANALEVQRRFMADASHELRTPLTSLRGNVDIMARNPDMPVEEQ
                     SAAVAELRAEAERMSRLVDGLLHLARGDLRADSQEPVLVDVGAALADQVVRVRRVASG
                     HEIRMDLPGAPAIVAARDGLIDRALGNLLDNAVVHGGANVLVSLRVRHGRATVRVRDD
                     GPGIAEHDLPHVFERFYRGAAGRSRPGTGLGLAIAAAAARGLGGDLTAVSRPGETTFE
                     LGLPVADELRPPVAGDRPAGRRPRAR"
BASE COUNT          419 a         1169 c         1043 g          375 t
ORIGIN      
        1 gcccccggtc cgggcccatg ctgaagccgc gccgctcctc ctctcgtcgt acccaggcgt
       61 cgggcaggcc cgaatggata cgggcaccgg gcaggtttcc catgcgatcc gggccgcggg
      121 gggcacgcag cgggtcaggc cgcgggcatg gcgatctgct gatccagggt tgacggaggc
      181 gtgtaaccgg gggcggccct cctggcccgg ggcatcgggg tggtccccca ggaccggtag
      241 gtgcccctga tcgccggggt cgagccgttg agcagggaca cgagtcgcgc cctgtaggac
      301 gccgacgggg tgaacacccg gctcatctcc gcgtagaccc ggaccgcgtt ggcgatgtag
      361 aagaggtcga tcgagggggt catcacgttc cagtcgcagc cgatgaagcg gacggagggc
      421 tggacccagg cggcggtcgg gaacgggttg gcgtagcgga tcatcgtgac ccgctccagg
      481 ccgaaccggt cccgtgacgt ggccggccgg cggacctccc accgcgtgcc cgtgaacgac
      541 cagccgacca cgttgctggt cgcgccctgc gagccgtccc gcggcggcac gtggaagtac
      601 aacgtcggct tccgcacgat gaacgacccg cccgccaccg tgacctgccc cccgctggtc
      661 acgttgaaca ccgcgccgtc ctcgagcgcg ggatgcgacg accaggtgcc gagccgcgcc
      721 gcgtaggcgg cgtccgggaa gctcaccccg tacgtcacct cgccccccac cagctgatgg
      781 ttcaccgcct gcgagttcgc atagtcgaag tcgatgaagt tgtcccggcc gtggaaccgc
      841 tccaccacca gcgagtcggt catctcgtcg ccctcgaacc ggaacgcccg gtgcagctcg
      901 ttgatctcca cccccgccac gtcgttcatc cacgtcgacc cgcccgtggg cgccccgtcc
      961 ccggacgact cgcggaacag gaacccgccc gacccctcca ccagcccccg cagcccgccc
     1021 accggcccag ccgggctcac cccctgcacc gtgaaatccg agaatgtcag cccacccgac
     1081 cggtgcacca cgaacaggaa gttcgcgccc tcatgctgga tgaccgtcgc gcccggcccg
     1141 tcgccgcgca ccgtgaaccc ctgcacgaac tcgatcgggt gcgacgccgt gttccggtag
     1201 atccccctcg ggatgtagag cgtcggcagc ccgagcaccc ggtacgaccc cgacccgccc
     1261 accgggtacc gccacgacgc cacgctctcg cagtacgaga tcgccgccga gatcgccggc
     1321 gtccagtccc acgtgttggg attcccagga tccggcctcg acaccacctg gtcggcgaac
     1381 tgccacaggt tcacctgctc gtgcccggcc acctcggaac gcgccggccc gacctgcgcc
     1441 gggctcacca gcaccgccac cgccaccccg caggcggcga ccacggccgc cacccgcacc
     1501 agcccgctcc gcaccagcca cgccatgccg ccgccacacc ccgactcttc gactcgcgat
     1561 ccaggtcgtg cggcggcagg caccgccgac gggcgtcggc cctccgcgat gtctgtgacg
     1621 ccattgcagc ccgccagcgc accggggcgg tattcgcact cctgcccacc tcctccacac
     1681 cactgcctag gcccacgggc taggtagtcc ggaggagggg cggctgcggc gggatccggg
     1741 aaaccggcta ccgtgcgcgg ggccgccgac ccgcgggccg gtcgcccgcc accggaggac
     1801 ggagctcgtc cgccaccggg aggccgagct cgaaggtggt ctcccccggc ctcgagacgg
     1861 cggtgaggtc gccgccgagg ccgcgcgctg ccgcggcggc gatggccagg ccgagcccgg
     1921 tccccgggcg gctcctgcca gccgcgccgc ggtagaagcg ctcgaacacg tgcggcaggt
     1981 cgtgctcggc gatgccgggt ccgtcgtcgc ggacgcgcac ggtcgcccgc ccgtgccgga
     2041 cgcggaggga cacgaggacg ttggcaccgc cgtggacgac cgcgttgtcg agcaggttgc
     2101 cgagcgcccg gtcgatcagg ccgtcccggg cggccacgat ggccggggcg ccgggcaggt
     2161 ccatccggat ctcgtgcccg gaggcgaccc ggcggacgcg gacgacctgg tcggcgaggg
     2221 cggccccgac gtcgaccagg accggctcct ggctgtcggc ccggaggtcg ccccttgcca
     2281 ggtggagcag cccgtccacc aggcggctca tccgctcggc ctcggcccgc agctcggcga
     2341 cggcggccga ctgctcctcg accggcatgt ccgggttgcg tgccatgatg tccacgttcc
     2401 cgcggaggct ggtcagcggc gtgcgcagct cgtgggaggc gtcggccatg aagcgccgct
     2461 ggacctcgag cgcgttcgcc agctccgcct gggccacctc caggcgggcg agcatgcggt
     2521 tcagcgtccg ggcgaggtcg ccgacctcgt cgtgggtgcc ggtcgcgacc cgcttggagg
     2581 ggtcgccgct gtcggccacc gccctcgccg cggcgctgat ggcggtgacg ggccgcatgg
     2641 cggcccggcc gaccacccac gccaataccg cggccagcgc cagggcgagc aggcccgagc
     2701 cgaccagcag gcgctggacc cggccgacgg tcgcccgcgt gttgtcgatc aggccgaggg
     2761 tgaccgcgcc aatgcgaccg aagccgatga cgttgaccgg cgcggtgatc gtccgcaccg
     2821 gcaccccctc gagccgcacg ctggcgtacc tggagcccgc ctcgcccctg gccaccggga
     2881 tcaccacggc cagaccggcg atcgcctccg gccgggtcga gccggcgatc ggccggccgt
     2941 cgctgtcgag cacctggaac agcgtgcccg ggatgcgctg ctcgaacggc aggccggggt
     3001 agttct
//
LOCUS       DASPYY010000340         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1041960, whole genome shotgun sequence.
ACCESSION   DASPYY010000340 DASPYY010000000
VERSION     DASPYY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041960"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VEP73_05195"
     CDS             <1..189
                     /locus_tag="VEP73_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HYT25862.1"
                     /translation="CLDVLSVAEVSARLDLPTNVVRILVCDLAEAGAVRVHQPAASHG
                     VPDLELLARVLGGLKAMR"
     gene            complement(231..1451)
                     /locus_tag="VEP73_05200"
     CDS             complement(231..1451)
                     /locus_tag="VEP73_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYT25863.1"
                     /translation="MAQTTFAYKVRDRSGRMIGGTLDADSQGTVAARLRQMGYAPVSI
                     EVAKQAALRKELSLPGMGRVKPKDLAVFSRQFATMINSGLSLLRALSILGDQTQNKRL
                     AGVVLEVRDEVEKGTALSAALARHPKVFNRLYLSMVRAGEVGGFLDQVLVKLAETLEK
                     EVELRGKVKSAMTYPVVVFAMVLLIVTAMLIFIVPMFKNLYGQLGGTLPLPTRVLVGV
                     SDLVKRGFVFVLPGIVVVTFGFRRLLATPSGRYRLDAFKLRAPVFGVLFHKTALSRFS
                     RTLATLLRSGVPILQALEIVSDTVNNGVMAKAIKDVQDSVREGESLAGPLEKHAVFPP
                     MVVQMMAVGEETGALDTMLTKVADFYDQEVEAVVNALTSLIEPILIAVMGAAVGGMVI
                     ALYMPMFNIINLVK"
     gene            complement(1470..2615)
                     /locus_tag="VEP73_05205"
     CDS             complement(1470..2615)
                     /locus_tag="VEP73_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYT25864.1"
                     /translation="MDPADQLLASRPPAGSARARAEEPEVRVAVAELLEAVLETGASD
                     LHLSAGARPAVRVHGHLGRLERYPVLVPNEIQSLIYSILTQRQREALERDRELDCAYT
                     LPGRARFRLNVYYQRNAVGAAFRLIPMDVQPLEDLGIPPQVASLSRLSRGFVLVTGPT
                     GSGKSTTLASLVDLANRERSDHIMTVEDPIEFLHKHKRCLVNQREVGEDTRSFASALK
                     HVLRQDPDVILVGEMRDLETISIALTAAETGHLVFATLHTQDAPQTIDRVIDVFPTNQ
                     QQQVRVQLAGAVQGVICQQLVPTADGAGRAVAVEVMVATPAIRNLVREGKTHQIYSAM
                     QAGGRHGMQTMDQSLADLVRRFRITYELGLERCHHVEDFNRLCGRAV"
     gene            complement(2752..3813)
                     /gene="mltG"
                     /locus_tag="VEP73_05210"
     CDS             complement(2752..3813)
                     /gene="mltG"
                     /locus_tag="VEP73_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYT25865.1"
                     /translation="MISRVERRVHRRRRARGLAVVTTFLVLVGGVLFGALKLSGGQGG
                     PKVAPGRKVTVTVLPGESTKQIAEQLERLGVVDGAGRFRRVAEDRGLDGALRPGTYQL
                     TTGMQPDQVLDVMARGPQGITLTVPEGFTLNQIVERLVTVGRFKRADVLAAVHDPMLA
                     TPYRPKGVSSLEGLLFPLTYQVNKGDTPVSVLQEMLDQLGEVLADTDFSHPRRGVRVT
                     PYQVLVVASLVEREAKVPADRPKVAAVIYNRLRHDIPLQVDPTVQYAWRLRNRVKPRL
                     SLADLRITSPFNTYTHHGLPPTPICSPGQDAIQAALNPANGEWLYYIGVSTDGSLRFT
                     SSYQEFLRLKQKARAAGVA"
     gene            complement(3810..4247)
                     /gene="ruvX"
                     /locus_tag="VEP73_05215"
     CDS             complement(3810..4247)
                     /gene="ruvX"
                     /locus_tag="VEP73_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYT25866.1"
                     /translation="MTGRVLGLDVGSRRLGVALSDPSGTVASPLVTLPRRGPPEDALA
                     LGRLAAEHRAATVVAGLPLTLAGREGPAAHAVRAYLGELQALLPGLDFELVDERLSTA
                     AAERALISGGVRRQERRAVVDQVAASLFLQTWLDAARQAGGPG"
     gene            complement(4244..>4449)
                     /locus_tag="VEP73_05220"
     CDS             complement(4244..>4449)
                     /locus_tag="VEP73_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HYT25867.1"
                     /translation="LADAGLAARDVLLPAAREVGGGAGGRGDLATAGGRDPSRLAEAL
                     AVARAEARRRVGALDEAQLPGPG"
BASE COUNT          597 a         1578 c         1656 g          618 t
ORIGIN      
        1 tgcctcgacg tcctctcggt cgcggaggtc tcggcgcggc tcgacctccc caccaacgtc
       61 gtccgcatcc tcgtctgcga cctggccgag gcgggcgcgg tcagggtgca ccagccggcc
      121 gcctcgcacg gggtgccgga cctcgagctc ctggcgcggg tgctcggcgg cctcaaggcg
      181 atgcggtaga cgccgggacc gtccgggacc gtccgcgagc attcggtcgt ctatttgacc
      241 aggttgatga tgttgaacat gggcatgtac agcgcgatca ccatcccgcc gaccgccgcg
      301 cccatgacgg cgatcaggat gggctcgatc aggctcgtca gcgcgttgac gaccgcctcg
      361 acctcctggt cgtagaagtc ggcgaccttg gtcagcatcg tgtccagggc gccggtctcc
      421 tccccgaccg ccatcatctg gacgaccatg ggcgggaaca ccgcgtgctt ctcgagcggc
      481 ccggcgaggc tctcgccctc ccggaccgag tcctggacgt ccttgatcgc cttggccatg
      541 acgccgttgt tgacggtgtc ggagacgatc tccagggcct gcaggatcgg cacgcccgac
      601 cgcagcagcg tggcgagcgt ccgcgagaag cgggagagcg cggtcttgtg gaacaggacg
      661 ccgaacacgg gcgcccgcag cttgaacgcg tcgagccggt agcggcccga cggggtcgcg
      721 agcaggcgcc tgaacccgaa ggtgacgacc acgatgccgg ggagcacgaa gacgaacccg
      781 cgcttgacga ggtcggagac cccgacgagg accctggtcg gcagcggcag ggtgcccccg
      841 agctgcccgt agaggttctt gaacatcggc acgatgaaga tcagcatcgc ggtcacgatg
      901 agcagcacca tggcgaagac gaccaccggg taggtcatgg ccgacttgac cttgccgcgc
      961 agctccacct ccttctcgag ggtctccgcg agcttgacga gcacctggtc gaggaagccg
     1021 ccgacctcgc cggcgcggac catcgagagg tagaggcggt tgaagacctt cgggtgcctg
     1081 gcgagggcgg ccgagagcgc ggtgcccttc tccacctcgt cgcggacctc gaggacgacg
     1141 ccggcgaggc gcttgttctg ggtctggtcg ccgaggatcg acagcgcgcg cagcagcgag
     1201 aggccggagt tgatcatcgt ggcgaactgc cgcgagaaca cggccaggtc cttgggcttc
     1261 acgcggccca tccccgggag cgacagctcc ttccgcaggg ccgcctgctt cgccacctcg
     1321 atggagacgg gcgcgtagcc catctggcgg aggcgggccg cgaccgtgcc ctggctgtcg
     1381 gcgtcgagcg tgccgccgat catcctgccc gagcggtcgc ggaccttgta ggcgaaggtc
     1441 gtctgggcca tggtggctcc tctcccccgc tacaccgccc gcccgcagag gcggttgaag
     1501 tcctccacgt gatggcagcg ctcgaggccg agctcgtagg tgatgcggaa gcgacggacc
     1561 aggtcggcca gggactggtc catcgtctgc atgccgtgcc tgccgcccgc ctgcatcgcc
     1621 gagtagatct ggtgggtctt gccctcgcgg acgaggttgc ggatggccgg cgtggcgacc
     1681 atcacctcga ccgcgaccgc ccgcccggca ccgtcggcgg tcgggacgag ctgctggcag
     1741 atcacgccct ggacggcgcc ggcgagctgc acccgcacct gctgctgctg gttggtcggg
     1801 aacacgtcga tgacccggtc gatggtctgg ggcgcgtcct gggtgtggag ggtcgcgaac
     1861 acgaggtgcc cggtctcggc cgcggtgagc gcgatcgaga tggtctcgag gtcgcgcatc
     1921 tccccgacga ggatgacgtc ggggtcctgg cgcaggacgt gcttgagcgc ggacgcgaac
     1981 gacctggtgt cctcccccac ctcgcgctgg ttgacgaggc agcgcttgtg cttgtggagg
     2041 aactcgatgg ggtcctcgac ggtcatgatg tggtcggacc gctcgcggtt ggcgaggtcg
     2101 accagtgacg cgagcgtggt cgacttcccc gacccggtcg gcccggtgac gagcacgaac
     2161 ccgcgcgaca ggcgcgagag gctggccacc tggggcggga tgccgaggtc ctcgagcggc
     2221 tgcacgtcca tcgggatgag ccggaaggcg gcgccgaccg cgttccgctg gtagtagacg
     2281 ttgagccgga agcgggcgcg gccgggcagc gtgtaggcgc agtccagctc gcggtcgcgc
     2341 tcgagcgcct cgcgctgccg ctgggtgagg atcgagtaga tcagcgactg gatctcgttc
     2401 ggcaccagca ccgggtagcg ctccagccgg ccgaggtgcc cgtggacccg cacggcgggc
     2461 ctggccccgg ccgacaggtg caggtccgac gcgcccgtct cgagcacggc ctcgagcagc
     2521 tcggcgacgg cgacccgcac ctcgggctcc tcggccctgg cgcgggcgct cccggccggc
     2581 gggcggctcg cgagcagctg gtcggctggg tccatcggtc gcacgcccct cgggtcgcgg
     2641 gaactggccg gcgggcggcc cggggtcgcg ggcgcccgta ccgtccgatg tcggccggag
     2701 gcccgggggc cttgaggccg gcgccggccc ggtgtgccgc cggcaccggc gtcacgccac
     2761 ccccgcggcc ctcgccttct gcttgagccg caggaactcc tggtagctcg aggtgaaccg
     2821 gaggctgccg tcggtggaga cgccgatgta gtagagccac tccccgttcg ccgggttcag
     2881 ggcggcctgg atcgcgtcct gcccgggcga gcagatgggg gtgggcggca ggccgtggtg
     2941 ggtgtaggtg ttgaacggcg aggtgatccg caggtcggcg aggctgagcc tgggcttcac
     3001 ccggttccgc aggcgccagg cgtactggac ggtcgggtcg acctgcaggg ggatgtcgtg
     3061 gcgcagccgg ttgtagatga ccgcggccac cttgggccgg tcggccggca ccttcgcctc
     3121 gcgctcgacc aggctcgcga cgacgaggac ctggtagggg gtgacccgca cgccgcgcct
     3181 ggggtgtgag aagtcggtgt cggcgaggac ctccccgagc tggtcgagca tctcctggag
     3241 gaccgagacc ggggtgtccc ccttgttgac ctggtacgtc agggggaaga gcaggccctc
     3301 gagcgacgag acccccttgg gccggtaggg cgtcgcgagc atggggtcgt ggacggcggc
     3361 caggacgtcc gcgcgcttga agcggccgac cgtgacgagc ctctcgacga tctggttgag
     3421 ggtgaaaccc tcggggaccg tcagcgtgat gccctggggg cccctggcca tcacgtcgag
     3481 cacctggtcg ggctgcatcc cggtggtgag ctggtaggtg cccggccgga gcgcgccgtc
     3541 gaggccccgg tcctcggcca cccggcggaa gcgcccggcg ccgtccacca ccccgagccg
     3601 ctccagctgc tccgcgatct gcttggtgct ctcgccgggg agcaccgtga ccgtcacctt
     3661 cctgcccggc gcgaccttcg gcccgccctg gccgccggag agcttcagcg ccccgaacag
     3721 cacgcccccc accaggacca ggaacgtcgt cacgacggca aggccgcgtg cacggcggcg
     3781 ccggtgcacg cgccgctcca ccctcgagat catccgggac ctcccgcctg gcgggccgcg
     3841 tccagccagg tctgcaggaa caggctcgcg gccacctggt ccaccaccgc ccgtctctcc
     3901 tggcggcgga cccccccgga aatcagcgcg cgctcggccg ccgcggtcga cagccgctcg
     3961 tccaccagct cgaagtcgag gccgggcagc agcgcctgga gctcgcccag gtacgcgcgc
     4021 acggcgtgcg ccgccgggcc ctcccggccc gccagcgtga gcggcagccc ggccacgacc
     4081 gtcgccgccc ggtgctcggc ggccagccgg ccgagcgcga gcgcgtcctc ggggggcccg
     4141 cgcctcggca gggtgaccag cggcgaggcg accgtgccgc tcgggtcgga cagggcgacg
     4201 ccgagccggc ggctgccgac gtcgaggccg aggacgcgac cggtcatccg ggccctggca
     4261 gctgggcctc gtccagcgcc ccgacgcgcc gtcgcgcctc cgcgcgcgcc acggccagcg
     4321 cctcggcgag ccgcgacggg tcccgcccgc cggcggtggc gaggtcgccc ctgccgccgg
     4381 ccccgccgcc gacctcgcgg gcggccggca gcagcacgtc gcgggcggcc agcccggcgt
     4441 cggcgagat
//
LOCUS       DASPYY010000341         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2025622, whole genome shotgun sequence.
ACCESSION   DASPYY010000341 DASPYY010000000
VERSION     DASPYY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025622"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..614)
                     /locus_tag="VEP73_05225"
     CDS             complement(150..614)
                     /locus_tag="VEP73_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYT25868.1"
                     /translation="MHRHPALQRHEEERRHSLQTRVADAITAFAGSMPFVYLHAVWFT
                     LWIGLRVEPFPFGLLTMIVSLEAIFLSTFVMLSQNRADKRRQVLADHQWELVQHEERQ
                     NDELLRLSEQILDLTSAVHQLTVTTTGREAIAVDAVPDAPSAAIGHETTASC"
     gene            complement(678..944)
                     /locus_tag="VEP73_05230"
                     /pseudo
     CDS             complement(678..944)
                     /locus_tag="VEP73_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            1155..2828
                     /gene="ettA"
                     /locus_tag="VEP73_05235"
     CDS             1155..2828
                     /gene="ettA"
                     /locus_tag="VEP73_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HYT25869.1"
                     /translation="MAQYIYQMQKVRKAHNDKLILDDVTLAFFPGAKIGVVGPNGAGK
                     SSVLKIMAGLDTVSNGEAVLTPGYSVGILLQEPELDPAKDVLGNVEEGVAPTRALLER
                     FEEIGAQLGAADPDTMEKLLDEYGRVQDAIEAAGAWDLDAQLEMAMDALRLPPGDADV
                     GTLSGGERRRVALCRLLLQRPDLLLLDEPTNHLDAESVQWLEQHLAAYPGAVLAVTHD
                     RYFLDNVAGWILELDRGRAYPYQGNYSTYLETKTKRLAAETRAGEGRRKVLERELEWV
                     RAGTRGRHAKGRARLNRYDELAAEAERGRTVDFDEIQIPAGPRLGDLVIEARGLAKGY
                     GDRLLFDDLAFTLPRGGIVGVIGANGAGKTTLFKLVTGEEQPDAGELRVGPTARLAYV
                     DQARAGLDPSRTVFEAVSGGLDHIRVGGREMPSRAYVGTFGFKGPDQQKPVGALSGGE
                     RNRLNLALTLRTGGNVLLLDEPTNDLDVDTLRSLEDALLSFAGCVIVVSHDRWFLDRI
                     ATHMLAFEGDSRVSWFEGNYADYEADKVRRLGAEAARPHRVTYRKLVRR"
     gene            complement(2901..>3596)
                     /locus_tag="VEP73_05240"
     CDS             complement(2901..>3596)
                     /locus_tag="VEP73_05240"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYT25870.1"
                     /translation="ELCLDTEGAVWILLHSGSRGIGNQLAQVHMARAKRLAHNADLPD
                     PDLAVFLSGTPEMAAYRHDLDWAQEYARHNRLAMLDLIRDTVRTVLGRPVGASLTVHC
                     HHNYVALEHHFGEDVLLTRKGAIRAGRGELGIVPGSMGAPSFVVRGLGNQNAFESAPH
                     GAGRAMSRNAARRRFTRDDLATAMRGIEARTDAGVLDEHPGAYKDVRKVIEQSRNLVE
                     VVAELRQVAVVKG"
BASE COUNT          521 a         1278 c         1324 g          473 t
ORIGIN      
        1 gcagaccatg cagccgaccc gggtgtcgct gtggctgcgg ccgccgccac cgcacggatc
       61 gcccaacgcg gccggagacg gcgcctcagc gcggcaacga acctaggcga aggtccgcag
      121 cgcctccacg gactcccaga ccgacaggtt caacaggatg cggtcgtctc gtgcccgatc
      181 gccgccgagg gggcgtccgg gacggcgtcc accgcgatcg cctcacgtcc ggtcgtcgtc
      241 accgtcagct ggtgcaccgc gctggtcagg tcgaggatct gctccgacag ccgcagcagc
      301 tcgtcgttct gccgctcctc gtgctgcacc agctcccact ggtggtcggc gaggacctgg
      361 cgccgcttgt ccgcccggtt ctggctgagc atcacgaagg tggacaggaa gatcgcctcg
      421 agcgacacga tcatggtcag caggccgaac gggaacggct ccacgcggag cccgatccac
      481 agggtgaacc acacggcgtg gaggtagacg aagggcatcg agccggcgaa cgcggtgatc
      541 gcgtccgcga cgcgagtctg gaggctgtgg cggcgctcct cctcgtgacg ctgcagcgcc
      601 gggtggcggt gcaccgggtc gcggatggac acgatgccga cgagcctgcc gccgtcgacc
      661 acggggagat gccggatctg ccggtcggcc atgagctggg cggcctcggc gatgtccgtc
      721 ttcggcccca ccgtgacgac gttcccgctc attcgcgccc cgaccgtcac ctcggtcggg
      781 tcctggccgt cggcggcgat cttgacgtag tcgcgttcgg tgaggatccc ggcgggaccg
      841 ccgtccccgg ggacaaggac cgacgagatg ccgtgctcgc gaagcacccg cgccgcctcg
      901 gccagggtcg cgtccgcgcc gatcgtcacc acgttccggt gcatgatgtc gacgacccgc
      961 acagccgacc tcccgtgagc agcccagccg gagcaccggg gcagatgcac cggccgccat
     1021 tctcacctcg tgctcatgag gggagggtga gccggcgggc ggcttccgcc agaccggggg
     1081 gacggccgga tcggcggatc cttggcatcc tgggggccgg cggcaggtcg gacacgagtg
     1141 gacgggacga cgacgtggcg cagtacatct accagatgca gaaggtgcgc aaggcgcaca
     1201 acgacaagct cattctggac gacgtcacgc tggcgttctt ccccggcgcg aagatcggcg
     1261 tggtgggacc gaacggcgcg ggcaagtcga gcgtgctgaa gatcatggcc gggctcgaca
     1321 ccgtgtccaa cggcgaggcg gtcctcacgc ccggctactc ggtcggcatc ctgctccagg
     1381 aaccggagct cgacccggcc aaggacgtgc tcggcaacgt ggaggagggc gtggcgccga
     1441 cccgcgcgct gctcgagcgc ttcgaggaga tcggcgccca gctcggcgcc gccgaccccg
     1501 acacgatgga gaagctgctg gacgagtacg gccgggtgca ggacgcgatc gaggcggccg
     1561 gcgcgtggga cctcgacgcc cagctggaga tggcgatgga cgcgctgcgg ctgccgccgg
     1621 gggacgccga cgtcggcacc ctgtcgggcg gcgagcggcg ccgggtcgcg ctctgccggc
     1681 tgctcctgca gcggcccgac ctgctgctgc tcgacgagcc cacgaaccac ctggacgccg
     1741 agtcggtcca gtggctcgag cagcacctcg ccgcctaccc gggcgccgtg ctcgcggtga
     1801 cccacgaccg ctacttcctc gacaacgtcg ccggctggat cctcgagctc gaccgcggcc
     1861 gcgcctaccc ataccagggg aactactcga cctatctgga gacgaagacg aagcggctgg
     1921 cggccgagac gcgtgccggc gaggggcgcc gcaaggtgct cgagcgggag ctcgagtggg
     1981 tgcgggccgg caccaggggc cgccacgcca agggcagggc ccgcctgaac cggtacgacg
     2041 agctggccgc cgaggccgag cggggccgga ccgtcgactt cgacgagatc cagatcccgg
     2101 ccggcccccg gctcggcgac ctcgtgatcg aggcgcgggg gctcgccaag gggtacggcg
     2161 accggctgct cttcgacgac ctcgcgttca ccctgccgcg gggcgggatc gtcggggtca
     2221 tcggcgccaa cggcgccggc aagaccaccc tgttcaagct ggtcaccggc gaggagcagc
     2281 ccgacgccgg cgagctgcgg gtcgggccga cggcgcggct cgcctacgtg gaccaggcca
     2341 gggccggcct cgacccgtcg aggacggtgt tcgaggcggt ctcgggcggc ctcgaccaca
     2401 tccgggtggg cggccgggag atgccctcgc gggcgtacgt ggggacgttc gggttcaagg
     2461 gcccggacca gcagaagccg gtgggggcgc tgtccggcgg cgagcgcaac cgcctgaacc
     2521 tggcgctcac cctccgcacc ggcggcaacg tcctgctgct cgacgagccg accaacgacc
     2581 tcgacgtcga caccctccgc tccctggagg acgcgctgct ctccttcgcg ggctgcgtga
     2641 tcgtggtcag ccacgaccgc tggttcctcg accggatcgc gacccacatg ctcgcgttcg
     2701 agggcgacag ccgggtgtcg tggttcgagg gcaactacgc cgactacgag gcggacaagg
     2761 tgcggcgcct cggcgccgag gccgcccgcc cgcaccgcgt cacctaccgc aagctggtcc
     2821 gccgctgagc acgccgaagc tggtccgccg ctgagcatcc cgaagctggt ccgccgccga
     2881 gcaccccggc agcgcccggg tcagcccttg acgacggcga cctggcggag ctcggcgacg
     2941 acctcgacga ggttgcgcga ctgctcgatc accttgcgca cgtccttgta cgcgccgggg
     3001 tgctcgtcga ggaccccggc gtcggtgcgg gcctcgatgc cccgcatcgc ggtggccagg
     3061 tcgtcgcggg tgaagcggcg gcgcgcggcg ttgcggctca tcgcccggcc ggcgccgtgc
     3121 ggggccgact cgaacgcgtt ctggttgccc aggccgcgga cgacgaacga cggcgcgccc
     3181 atcgagcccg ggacgatgcc gagctcgccc ctgccggccc ggatcgcccc cttgcgggtc
     3241 agcagcacgt cctcgccgaa atggtgctcg agcgcgacgt agttgtggtg gcagtgcacc
     3301 gtcagcgagg cgccgacggg ccggccgagc accgtccgga ccgtgtcgcg gatcaggtcg
     3361 agcatggcca ggcggttgtg ccgcgcgtac tcctgcgccc agtccaggtc gtggcggtag
     3421 gccgccatct cgggcgtccc ggacaggaac acggccaggt cggggtcggg caggtcggcg
     3481 ttgtgggcga gccgcttcgc gcgggccatg tggacctggg cgagctggtt gccgatgccg
     3541 cgcgacccgg agtgcagcag gatccagacc gcgccctcgg tgtccaggca cagctc
//
LOCUS       DASPYY010000342         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2165791, whole genome shotgun sequence.
ACCESSION   DASPYY010000342 DASPYY010000000
VERSION     DASPYY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165791"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VEP73_05245"
     CDS             complement(<1..873)
                     /locus_tag="VEP73_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT25871.1"
                     /translation="MTAAAPDALDLLHPVVLHHIVNTLGWPGLRPLQEASIEPLLAGD
                     DALLLAPTAGGKTEAAAFPLLSKMASEGWQGTSLLYVCPLKALLNNLLPRLETYTGWL
                     GRRAALWHGDVTSTRRRQILRDQPDVLLTTPESLESMLVSVNVDHRRLFSGLRAIVVD
                     EVHAFAGDDRGWHLLAVLERLSRVTGRRVQRVGLSATVGNPDELLRWLQGSAAGSRRA
                     HVLARRQDAALTVAKGSAPRDTPPGNVQVDYVGSVANAATVIAALHRGEKRLVFCDSR
                     KLVEELGQALRARGV"
     gene            complement(870..2177)
                     /gene="brxD"
                     /locus_tag="VEP73_05250"
     CDS             complement(870..2177)
                     /gene="brxD"
                     /locus_tag="VEP73_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HYT25872.1"
                     /translation="MSAARRREVVDALRRGAVPEAGLDLFAVGLARFEMAVDEDLAAV
                     ASGGAVFKAVRGEYGSGKTFFSRWLGERAKRLNLATAEVQVSETETPLHRLETVYRRL
                     TERLTTPSFPPSALRPVVDAWFYALEDDVLAGGTVDATDAGALDREVAALLTQRLAAV
                     SRTAPTFAAALRGYRAALLQGDQATAEAVLAWLGGQPNVAAAARRAAGVRGDLDHFGA
                     LGFLQGLLAVLRDCGHPGLLFVLDEVETLQRVRSDARDKALNSLRQLIDEVHSGRFPG
                     LYLVITGTPAFYDGQQGAQRLAPLAQRLATDFATDARFDNPRAVQVRLPGFTVDSLTE
                     LGGKVRDLYADGSRSPGRIASVVDDAYLADLARAVAGGLGGKVGVAPRLFLKKLVGDV
                     LDRVDQFPDFHPRRDYALTLSSAEMSEVERNAASADEVALDLP"
     gene            complement(2186..>3565)
                     /gene="pglZ"
                     /locus_tag="VEP73_05255"
     CDS             complement(2186..>3565)
                     /gene="pglZ"
                     /locus_tag="VEP73_05255"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HYT25873.1"
                     /translation="TAAGSSEPGGMLLVESVLPRVVAPLLGTAKRPALLLVLDGMSAA
                     VAVELAEELRRQRWEEYDPLHDGGADQEARRRAVVAALPTLTRVSRASLFAAGLRQGG
                     QDDEQKAFEGHALWRGRKVRLFHKDAVRGGAGEALGDELEQALSDPDTLVAVVLNTID
                     DALDHGREGADPGWEVADLGSLRALLDHARYHGRAVVLTSDHGHVLERGGELRAVPDA
                     LSARHRAGDGPVTDGEVELAGPRVVAPGNRVVALWDPDVRYLPRRAGYHGGASLAEVT
                     VPLLAFLPLGAAAPAGWRPLDDQHPAWWSLARVTPEAQPVPAAVPQPRKPRGRAPVPE
                     GQAALDLVAATAPSPEPPGGATLVDALLDSEMFAAQHGLTPRKVPASKIRAAITALLE
                     ANGVLPVTVLADRAGEHAARAGGFVTTLQRIFNVDNYPVLSVVDDGRTVRLDARLLRE
                     QFGLREGRG"
BASE COUNT          465 a         1311 c         1351 g          438 t
ORIGIN      
        1 gacgcccctg gcccgcagcg cctggcccag ctcctccacg agcttcctgg agtcgcagaa
       61 caccagccgc ttctcgcccc ggtgcagggc ggcgatcacg gtggcggcgt tcgccaccga
      121 gccgacgtag tcgacctgga cgttgcctgg cggcgtatcg cgcggggcgc tccccttggc
      181 gacggtgagc gccgcgtcct gccgacgtgc cagcacgtgc gcccggcggg atccggctgc
      241 cgacccttgc agccagcgca gcagctcatc ggggttgccg acggtcgccg acagcccgac
      301 ccgctgcacc cggcgtcctg tcacccgcga gagccgctcg aggacggcga gcaggtgcca
      361 gccccggtcg tccccggcga aggcgtgcac ctcgtccacg acgatcgcgc ggaggccgga
      421 gaagagccgc cggtggtcga cgttcacgct gaccagcatc gactccagcg actctggggt
      481 ggtcaggagc acgtccggct ggtcacgcag gatctgccgg cggcgggtcg aggtgacgtc
      541 cccgtgccag agggcggccc gccggccgag ccagccggtg taggtctcca gccgggggag
      601 caggttgttg agcaacgcct tgagcgggca gacgtacagc agggacgtgc cctgccagcc
      661 ctctgaggcc atcttcgaga gcagcgggaa cgccgccgcc tcggtcttgc cgcccgccgt
      721 gggggcgagc aggagcgcgt cgtccccggc gagcagcggc tcgatcgacg cttcctgcag
      781 cggccgcagc cccggccacc ccagcgtgtt gacgatgtgg tgcaggacca ccggatggag
      841 caggtcgagc gcgtcggggg cggcggcggt catgggaggt ccagggcgac ctcgtcggcg
      901 gaggcggcgt tgcgctcgac ctcgctcatc tcagcggacg acagggtgag ggcgtagtcc
      961 ctcctggggt ggaagtcggg aaactggtcc acccggtcca gcacgtcgcc gaccagcttc
     1021 ttcaggaaca gccttggggc gacgccgacc ttgccgccca ggcctccggc cacggcgcgg
     1081 gcgaggtcgg ccaggtaggc gtcgtcgacc accgaggcga tcctgccggg cgagcgcgag
     1141 ccgtccgcgt acaggtcgcg gaccttcccg cccagctcgg tgagcgagtc gacggtgaag
     1201 ccgggcagcc ggacctgcac cgcccgcggg ttgtcgaatc gggcgtcggt ggcgaagtcg
     1261 gtggccagcc gctgagcgag cggggcgagg cgctgcgcgc cctgctgccc gtcgtagaag
     1321 gccggcgtgc cggtgatcac caggtacagc ccggggaagc gacccgagtg gacctcgtcg
     1381 atgagctggc ggagtgagtt cagcgccttg tcgcgcgcgt cggagcggac ccgctgcagc
     1441 gtctccacct cgtccaggac gaacaacagc ccggggtggc cgcagtcgcg caggacggcg
     1501 agcagcccct gcaggaagcc gagcgcaccg aagtggtcga ggtcgccgcg caccccggcg
     1561 gcgcggcggg ccgcggcggc gacgttgggc tgcccgccga gccaggcgag gacagcctca
     1621 gcggtcgcct ggtccccctg gaggagggcc gcgcggtagc cgcgcagcgc tgcggcgaac
     1681 gtgggcgcgg tccggctcac cgcggccagg cgctgggtga ggagggctgc cacctcgcgg
     1741 tcgagcgcgc cggcatccgt cgcgtccacg gtgccgccgg cgaggacgtc gtcctcgagc
     1801 gcgtagaacc aggcgtccac gacggggcgg agcgcgctcg gcgggaagct cggcgtggtg
     1861 agccgctcgg tgagcctccg gtacacggtc tcgagccggt gcagcggggt ctcggtctcg
     1921 gagacctgca cctcggccgt tgccaggttc aaccgcttgg cgcgctcgcc cagccagcgg
     1981 ctgaagaacg tcttgccgga gccgtactcg ccgcgcacgg ccttgaagac ggcgccgccg
     2041 gacgcgacgg ccgcgaggtc ctcgtcgacc gccatctcga agcgggcgag gccgaccgcg
     2101 aagaggtcga gcccggcctc gggcacggcg ccccggcgca gcgcgtcgac cacttcccgc
     2161 cggcgtgccg cgctcacccg cgggctcacc cccgaccctc ccgcagcccg aactgctcgc
     2221 ggagcaggcg ggcgtccagc cggacggtcc ggccgtcgtc gaccacggag agcacggggt
     2281 agttgtcgac gttgaaaatg cgctgcaagg tggtgacgaa gccgcccgcc cgggcggcat
     2341 gctcgccggc ccggtcggcc agcacggtga ccgggagcac gccgttggcc tcgagcagcg
     2401 ccgtgatcgc cgccctgatc ttcgacgcgg ggaccttgcg gggcgtcagg ccgtgctggg
     2461 ccgcgaacat ctccgagtcg aggagcgcgt cgaccagcgt cgcgccgccg ggcggctcgg
     2521 gggagggtgc agtggccgcg accaggtcga gggccgcctg gccctccggc acgggtgccc
     2581 tgcccctcgg cttcctcggt tgcgggacgg ccgccgggac gggttgggct tccggggtca
     2641 cgcgcgccag cgaccaccat gccgggtgct ggtcgtcgag cgggcgccag ccagcggggg
     2701 cggccgcacc gagcggcagg aaggccagca gcgggacggt gacctcggcc agcgacgcgc
     2761 cgccgtggta cccggccctc cgcggcaggt agcgcacatc ggggtcccac agcgccacga
     2821 cacggttgcc cggggcgacc actcgcggcc cggccagctc gacctccccg tcggtgaccg
     2881 ggccgtcacc cgcgcggtgg cgggcggaga gcgcgtccgg caccgcccgc agctcccccc
     2941 ctcgttccag cacgtgcccg tggtcgctgg tgaggacgac cgcacggccg tggtagcggg
     3001 cgtggtcgag cagcgcccgg agcgatccga ggtcggcgac ctcccagccg gggtcggcgc
     3061 cctcgcgacc gtggtcgagc gcgtcgtcga tggtgttgag caccaccgcg accagcgtgt
     3121 ccggatcgct gagcgcctgc tccagctcgt ccccgagggc ctcgccggcc ccgccgcgca
     3181 cggcgtcctt gtggaacagg cggaccttgc ggccacgcca gagcgcgtgg ccttcgaacg
     3241 ccttctgctc gtcgtcctgc ccgccctggc ggagcccggc ggcgaacagc gacgcccggg
     3301 agaccctggt gagcgtcggc agcgccgcga ccaccgcacg gcggcgggct tcctggtccg
     3361 cgccgccgtc atgcagcggg tcgtactcct cccagcgctg gcggcgcagc tcctcggcca
     3421 gctccaccgc gacggctgcg ctcatgccgt ccaggaccag caggagcgcc ggccgcttgg
     3481 cggtcccgag cagcggcgcc acgacccgtg gcagcaccga ctcgacgagg agcatccccc
     3541 ctggttcgga ggagcccgcc gcggt
//
LOCUS       DASPYY010000343         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2307629, whole genome shotgun sequence.
ACCESSION   DASPYY010000343 DASPYY010000000
VERSION     DASPYY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307629"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1168)
                     /locus_tag="VEP73_05260"
     CDS             complement(56..1168)
                     /locus_tag="VEP73_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYT25874.1"
                     /translation="MVVTARAEHVGSLLRPPSLLEARQAHAAGTLDAAAFKAAEDRAV
                     REVIALQEQVGCPVVTDGELRRDSFQSELTAAVEGFAGVTMDAWLWGEWHSDVVGDAR
                     VEQPPDLGVVAPLRRRRNLAAEEFTFLRAHTDRIGKVTLPSPSLFANLWSPERSRDAY
                     PTMDDFLADVVAILCDEVRELNRLGCTYVQLDAPHYPLLIDPVWRAFYERHGWTLERW
                     LSHGIELDNAVIDAGRPATFGFHLCRGNQHSRWLVAGGYDAIARPVFGGIHADRLLLE
                     YDDERSGSFDPLRLVREDALVVLGLVTTKTPRAETEDELERRLREASRLTGLERLAVG
                     TQCGFATSVQGNAISVEDERRKLAVLVRTAERVFAA"
     gene            complement(1178..1396)
                     /locus_tag="VEP73_05265"
     CDS             complement(1178..1396)
                     /locus_tag="VEP73_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25875.1"
                     /translation="MADRVRLALVGLGRMGRAHLRALGAQVTAFAVAADSGRPRGATP
                     ADAIAALVAAGRAGQALRSAGDCAANPP"
     gene            complement(1458..>1652)
                     /locus_tag="VEP73_05270"
     CDS             complement(1458..>1652)
                     /locus_tag="VEP73_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25876.1"
                     /translation="DEVVSYEDPFRLELLAFHEAVASGHEPPTTVRDALRDVELCERI
                     VAAHLARGRGTGVPAPTGGA"
BASE COUNT          215 a          606 c          631 g          200 t
ORIGIN      
        1 tccacgaccg tggtcaatat gggcgtccgc gaccgtggtc aacaggccct ggaggtcatg
       61 cggcgaacac gcgctccgcg gtgcgcacca gcaccgcgag cttgcggcgc tcgtcctcca
      121 cgctgatcgc gttgccctgc acggaggtcg cgaacccgca ctgggtgccg acggccaggc
      181 gctccagacc ggtcagccgg ctcgcctcgc gcaggcggcg ctccagctcg tcctcggtct
      241 cggcccgcgg cgtcttggtc gtcaccagcc ccagcaccac cagcgcgtcc tcgcgcacca
      301 ggcggagcgg gtcgaagctc cccgagcgct cgtcgtcgta ctcgagcagc agccggtccg
      361 cgtggatgcc accgaacacc ggccgggcga tggcgtcgta gccgcccgcg accagccagc
      421 ggctgtgctg gttgccgcgg cacagatgga acccgaaggt cgccgggcgg cccgcgtcga
      481 tcaccgcgtt gtccagctcg atcccgtggg agagccagcg ctcgagcgtc cagccgtgcc
      541 gctcgtagaa ggcgcgccac accgggtcga tgagcagcgg gtagtggggg gcgtcgagct
      601 ggacgtaggt gcagccgagc cggttcagct cccgcacctc gtcgcagagg atcgccacca
      661 cgtcggcgag gaagtcgtcc atggtcgggt aggcgtcccg ggagcgctcg ggtgaccaca
      721 ggttggcgaa caggctcggg ctcggcaggg tcaccttgcc gatccggtcg gtgtgcgcgc
      781 gcaggaaggt gaactcctcg gcggcgaggt tgcgccgccg ccgcaggggg gcgaccaccc
      841 cgaggtccgg cggctgctcg acgcgcgcgt cgccgacgac gtcggagtgc cactcgcccc
      901 acagccaggc gtccatggtc acgccggcga agccctcgac ggccgcggtg agctcgctct
      961 ggaacgagtc ccggcgcagc tccccgtcgg tgacgacggg gcagccgacc tgctcctgga
     1021 gcgcgatcac ctcgcggacc gcgcggtcct cggccgcctt gaaggccgcc gcgtccagcg
     1081 tgccggcggc gtgcgcctgg cgggcctcca gcagggaggg cggccgcagg aggctgccga
     1141 cgtgctcggc acgggcggtc acgaccatgg cccaagctta cggcgggttc gcggcgcagt
     1201 cacctgcgga gcgaagcgcc tggcccgcgc gtccggcggc gaccagggcg gcgatcgcgt
     1261 cggcgggggt ggcgccccgc ggccggccgc tgtcggcggc gacggcgaac gccgtgacct
     1321 gggcgccgag cgcgcgcagg tgggcgcggc ccatgcggcc gaggcccacg agggcgaggc
     1381 ggacccggtc ggccatcccg ggtcctcaga agcctcgggc gcgcaggggg gcgcacctgg
     1441 cgcgccgcac ggtcacgtca cgcacctccg gtcggggcgg gaacgcccgt gccgcggccg
     1501 cgcgccaggt gcgcggcgac gatgcgctcg cacagctcga cgtcgcgcag cgcgtcgcgg
     1561 acggtcgtcg gcggctcgtg cccggaggcc accgcctcgt ggaaggcgag cagctcgagc
     1621 ctgaacgggt cctcgtagga gacgacctcg tc
//
LOCUS       DASPYY010000344         5215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1043032, whole genome shotgun sequence.
ACCESSION   DASPYY010000344 DASPYY010000000
VERSION     DASPYY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043032"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VEP73_05275"
     CDS             <1..440
                     /locus_tag="VEP73_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25877.1"
                     /translation="AFTPIALLKLIPLAEAAVVTAGHQRAALRQASAGATAFTGSSMV
                     TQMIQSRFRANAGGSSAGAASWGSPATAAVSAGSAAMTAARNRAESGFAAFGANAGGG
                     TGANGPRGDGVPSSGGATGASGGAATRPLRVGELASEGKSADA"
     gene            433..1932
                     /locus_tag="VEP73_05280"
     CDS             433..1932
                     /locus_tag="VEP73_05280"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HYT25878.1"
                     /translation="MRSAPEVRYRFGPLERRGLIGSLRPGQLAVLGAALLLTILIVQA
                     MKSGAGLGLGTVVLAGGGLASFVPVGGRSLNEWIPVAASFLGRRVTGRGRFRSRAPQA
                     GTRFKGGDVPVGLPDSIGKVELIAFPFRGVELGVIADRANGTYTATVQARARSFVLLD
                     PADKASRLAGWAGVVAGLAREGSPISRVQWIERTVPDDRNGLGRYLREAMDPAIGLDA
                     APLQSYLRLTQAAGPVTEAHELYVAVQINAGKAGRAIKQAGGKDTGACTVLARELETM
                     ARRLEGAEIEVLHALGPRRLAATIRLAFDPHSRSSLARLATVDPSREEGVSARNAWPR
                     STEAHWSYYRTNDVVHSTYWIAEWPRLDVGPDFLAPLLVQTRSMRSVSVTMEPVPALK
                     AMRAVGFAKTADFADEELRHKLGFLGTAKRRNQSEAVARREQELADGHADVRFSGWIT
                     VTADNLESLAEACGEVEHAAGQSRLELQRCDGEQDVCFSYTLPLGRGLK"
     gene            1929..3224
                     /locus_tag="VEP73_05285"
     CDS             1929..3224
                     /locus_tag="VEP73_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25879.1"
                     /translation="MRTKPRRAAHRVTTAHLQAAYPFISEGGLGSRGVYIGRDVFGGS
                     FCYDAWTLYGREITGPNMLVIGQIGYAKSSLVKTFLWRQMLFGRTAWVVDPKGEYDRL
                     AAVCGVEPIRFVTGGQIRLNPLDPMIGGDAQLALLRSIAGVMLGRNLRPEEDACLERA
                     HDEALIEASRRGAQPIVPQVVNRLLEPSDATAHELRTSPARLAEDGRQLALALRRMGA
                     GDLRGMFDGPTSRGLNLSGRLVVFNLRDVKDEARPILMACTAAWLQGSWARDDGVKRI
                     VVLDEAWVVLRHLAIARWLRESWKLARQYGVQNIAVMHRLSDLTAAGDSGSEQVQLAK
                     GLLEDSETRVIYRQSPGEVEAARELLGLTQTEVDQIPTLERGEALWRVGRRSFIVQHR
                     LDPVLEREIVDTDANMSTSSVVAGSWYGSGPPEAVEPAS"
     gene            3221..4957
                     /locus_tag="VEP73_05290"
     CDS             3221..4957
                     /locus_tag="VEP73_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HYT25880.1"
                     /translation="MSALKPVVKREGPLDQALLLGISIVLGAGIVLWLTGELTGWLHS
                     RHWPRVTAAEMGPVLVNLARHPGDPAAAWPAHASRLLPGPVAFYFVFGLLLLVLTAAL
                     MYANVAMRVVRRRLGGVIVVPGAAAAPGRRQQQPTWARPQAFRDLYVRESMPGRVTLG
                     RINGRPVAAEPLQSVIALGPPQSHKTSGLAVPAVFEWDGPVLAASIKADLIRTTIDQR
                     WQKGEVYLYDPTGITGLEQSSWSPLSRCGTWHGAYRTAVSLLSAGRPQSHAGEADFHH
                     SMAPNLLAPLLLAAAISGRTMGDVVRWVQRQERAEVTAALNVANEPAAQDAMQSVWSL
                     GDNRAALYASVLGAVSAYADPAVKEAVPATQLTAERLLDGGMNTAYVCAAAHEQRRLG
                     PLFVALLQEVVDLAYERSDHTGIPLNPPLLVVLDDAAVSAALPQLDMLASTAANHGVQ
                     LLTTFRTITQIQSRYGERAEAVFSNHRAKILLSGITDPPTLTLLSHLLEDETLRHVAP
                     TAPPAQEGAVVRARSGSASPAEALRRIYPGQGVLLYGHLPPAFLTLRPFFKDRRMAAR
                     SGDGRGVARRPI"
     gene            complement(4967..>5215)
                     /locus_tag="VEP73_05295"
     CDS             complement(4967..>5215)
                     /locus_tag="VEP73_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25881.1"
                     /translation="PDNLGCEGLTRVPDPAALVTNDGGLVTDATLGTPATTTTTQAPA
                     SSAAGSPTTLTGPTTTAGAGSTATTIGTGGATTTVPYP"
BASE COUNT          656 a         1919 c         1963 g          677 t
ORIGIN      
        1 ccgcgttcac cccgatcgcg ctgctcaagc tcatcccgct tgccgaggcg gcggtcgtca
       61 ccgccgggca ccagcgggcc gcgctgcgcc aggcgagcgc gggggcgacc gcgttcaccg
      121 gctcgtcgat ggtcacccag atgatccagt cgcgcttccg ggccaacgcc ggggggtcct
      181 cggccggcgc ggcgagctgg ggctcgccgg ccaccgcggc ggtctcggcc ggctccgcgg
      241 cgatgacggc cgcccgcaac cgcgccgagt cggggttcgc cgccttcggc gcgaacgccg
      301 gcggaggcac cggcgccaac ggcccgaggg gagacggtgt accgtcgtcc gggggggcga
      361 caggcgcctc cggcggcgcg gccacccggc cgctgagggt cggcgagctg gcgagcgaag
      421 gaaagagcgc ggatgcgtag tgcgcccgag gtccgctacc ggttcgggcc cctggagcgc
      481 aggggcctga tcggcagcct ccgccccggg cagctggcgg tgctgggcgc cgcactgctg
      541 ctcacgatcc tcatcgtgca ggcgatgaag agcggggcgg ggctggggct cggcacggtc
      601 gtgctcgccg ggggcggcct ggcgagcttc gtgcccgtcg ggggccgctc gctcaacgag
      661 tggatcccgg tggccgccag cttcctggga cgccgggtga ccgggcgcgg ccgcttccgc
      721 tcgcgcgcgc cccaggcggg cacccgcttc aagggcggcg acgtgccggt gggcctgccc
      781 gactcgatcg gcaaggtgga gctgatcgcc ttccccttcc gcggcgtgga gctgggcgtg
      841 atcgccgacc gcgccaacgg cacctacacc gcgaccgtgc aggcccgcgc ccgctcgttc
      901 gtgctgctcg accctgccga caaggccagc cggctggccg gctgggccgg ggtggtcgcc
      961 gggctggcca gggagggcag cccgatcagc cgggtgcagt ggatcgagcg gacggtgccc
     1021 gacgaccgca acggcctggg ccgctacctc cgcgaggcga tggaccccgc catcgggctg
     1081 gacgccgccc cgctgcagag ctacctgcgc ctcacccagg ccgccggccc ggtcaccgag
     1141 gcccacgagc tctacgtcgc cgtgcagatc aacgcgggca aggcaggccg cgcgatcaag
     1201 caggccggcg gcaaggacac cggcgcgtgc accgtgctcg cccgcgagct cgagaccatg
     1261 gcccgccggc tcgagggcgc cgagatcgag gtcctccacg cgctcggccc gcggcgcctg
     1321 gcggccacga tccggctcgc gttcgacccg cactcgcgct ccagcctcgc ccggctggcc
     1381 accgtcgacc cgagccggga ggaaggcgtc agcgcccgca atgcctggcc gcgctcgacc
     1441 gaggcacact ggtcgtacta ccgcaccaac gacgtggtgc actcgaccta ctggatcgcg
     1501 gagtggcccc gcctggacgt cggccccgac ttcctcgccc cgctgctggt gcagacccgc
     1561 tcgatgcgca gcgtctcggt gacgatggag ccggtgccgg cgctgaaggc gatgcgcgcg
     1621 gtcgggttcg ccaagaccgc cgacttcgcc gacgaggagc tgcgccacaa gctcggcttc
     1681 ctcggcaccg ccaagcgccg caaccagagc gaggcggtgg cccggcgcga gcaggagctc
     1741 gccgacggcc acgccgacgt ccgcttctcc ggctggatca ccgtcaccgc cgacaacctc
     1801 gagtcgctcg ccgaggcctg cggcgaggtc gagcacgccg cgggccagtc ccgcctcgag
     1861 ctccagcgct gcgatggcga acaggacgtg tgcttctcgt acacactgcc gctgggtcgt
     1921 gggctgaagt gaggaccaag cctcgccgag cggcgcaccg ggtcaccacc gcccacctgc
     1981 aggcggcgta cccgttcatc tccgagggcg ggctcggcag ccgcggcgtg tacatcgggc
     2041 gagacgtttt tggcggctcg ttctgctacg acgcgtggac gctctacggc cgggagatca
     2101 ccggcccgaa catgctcgtc atcgggcaga tcggctacgc caagagctcg ctcgtcaaga
     2161 cgttcctatg gcggcagatg ctgttcgggc ggaccgcgtg ggtggtcgat cccaaggggg
     2221 agtacgaccg gctggcggcg gtgtgcgggg tggagccgat ccggttcgtg accggcgggc
     2281 agatccggct gaacccgctc gacccgatga tcggggggga cgcccagctc gcgctgctgc
     2341 ggtcgatcgc cggggtgatg ctcgggcgga acctccggcc cgaggaggac gcctgcctgg
     2401 agcgcgcgca cgacgaggcg ctgatcgagg cgtcgcggcg cggcgcgcag ccgatcgtgc
     2461 cccaggtggt gaaccggctg ctcgagccgt cggacgcgac agcccacgag ctgcggacct
     2521 cgcccgcgcg gctggccgag gacggcaggc agctcgcgct ggcgctgcgg cggatgggcg
     2581 ccggcgacct gcgcgggatg ttcgacgggc cgacctcgcg cgggctgaac ctgtccgggc
     2641 ggctggtggt gttcaacctg cgcgacgtca aggacgaggc caggcccatc ctgatggcgt
     2701 gcacggcggc gtggctgcag ggctcgtggg cgcgtgacga cggcgtcaag cggatcgtgg
     2761 tgctcgacga ggcgtgggtg gtgctgcggc acctggcgat cgcgcgctgg ctgcgggaga
     2821 gctggaagct ggcgcggcag tacggggtgc agaacatcgc ggtgatgcac cgcctctcgg
     2881 acctgaccgc ggcgggggac tcgggcagcg agcaggtgca gctcgccaag ggcctgctcg
     2941 aggactccga gacccgggtg atctaccggc agtccccggg cgaggtggag gcggcccgcg
     3001 agctgctcgg gctgacccag accgaggtgg accagatccc caccctggag cggggcgagg
     3061 cgctgtggcg ggtcggccgc cgctcgttca tcgtgcagca ccgcctggac ccggtgctcg
     3121 agcgggagat cgtcgacacc gacgcgaaca tgtcgacgag ctcggtggtg gccgggagct
     3181 ggtacgggtc gggcccgccc gaggcggtgg agccggcctc gtgagcgcgc tcaagccggt
     3241 ggtcaagcgg gaggggccgc tcgaccaggc gctgctgctc ggcatctcga tcgtgctcgg
     3301 cgccgggatc gtgctctggc tgaccggcga gctcaccggc tggctgcact cccgccactg
     3361 gcccagggtc accgcggccg agatgggacc ggtgctcgtc aacctcgccc gccaccctgg
     3421 cgacccggcg gcggcgtggc ccgcgcacgc gagcaggctc ctgcccggcc cggtggcgtt
     3481 ctacttcgtc ttcgggctcc tgctcctggt gctgaccgcg gcgctcatgt acgccaacgt
     3541 cgccatgcgg gtcgtgcgcc ggcggctcgg cggggtgatc gtggtgcctg gcgcggccgc
     3601 ggcgcccggc cggcgccagc agcagcccac ctgggcgcgc ccgcaggcgt tccgcgacct
     3661 ctacgtgcgc gagtcgatgc cggggcgggt cacgctcggg cggatcaacg gccggccggt
     3721 ggccgccgag ccgctgcagt cggtgatcgc gctcgggccg ccccagagcc acaagacgtc
     3781 cgggctcgcg gtgccggccg tcttcgagtg ggacgggccc gtgctggccg cctcgatcaa
     3841 ggccgacctg atccgcacca cgatcgacca gcgctggcag aagggcgagg tgtacctgta
     3901 cgaccccacc ggcatcacgg ggctcgagca gagctcctgg tcgccgctca gccgctgcgg
     3961 gacctggcac ggcgcctacc gcaccgccgt gtccctgctc agcgccggcc ggccgcagag
     4021 ccacgccggc gaggccgact tccaccacag catggcgccg aacctgctcg cgccgctgct
     4081 gctggccgcc gcgatctcgg gccgcaccat gggcgacgtc gtccgctggg tccagcggca
     4141 ggagcgggcc gaggtgaccg ccgccctgaa cgtggccaac gagccggccg cccaggacgc
     4201 catgcagtcg gtctggagcc tcggcgacaa ccgggccgcg ctgtacgcga gcgtgctcgg
     4261 cgccgtgagc gcgtacgccg acccggcggt caaggaggcg gtgcccgcga cccagctcac
     4321 ggccgagcgc ctgctcgacg gcgggatgaa caccgcctac gtgtgcgcgg ccgcccacga
     4381 gcagcgccgg ctcggccccc tgttcgtcgc gctcctccag gaggtcgtcg acctggcgta
     4441 cgagcgctcc gaccacaccg ggatcccgct caacccgccc ctgctcgtcg tgctcgacga
     4501 cgcggccgtc tcggcggcgc tgccgcagct cgacatgctc gcgtcgaccg cggcgaacca
     4561 cggcgtgcag ctcctgacca ccttccggac catcacccag atccagtccc gctacggcga
     4621 gcgcgccgag gcggtgttct ccaaccaccg cgcgaagatc ctcctgagcg gcatcaccga
     4681 cccgccgacg ctcaccctgc tctcgcacct cctcgaggac gagacgctcc gccacgtcgc
     4741 cccgaccgcc ccgcccgccc aggagggcgc ggtggtccgg gccaggtcgg gcagcgcctc
     4801 gccggccgag gcgctgcgcc ggatctaccc gggccagggc gtcctgctct acgggcacct
     4861 gcccccggcg ttcctgaccc tgcggccctt cttcaaggac cgcaggatgg ccgcgaggag
     4921 cggggacggc aggggcgtcg cgcggcgccc catctgagtc ccgctcctac gggtagggca
     4981 cggtcgtggt ggcgcccccg gtgccgatgg tggttgcggt gctccccgcg ccggccgtcg
     5041 tggtcgggcc ggtgagggtc gtgggcgaac ccgccgcgct gctcgccggg gcctgggtgg
     5101 tggtcgtggt cgccggggtg cccaatgtcg cgtcggtcac gaggccgccg tcgttggtca
     5161 cgagcgccgc ggggtcgggg acgcgggtca gcccctcgca gcccaggttg tcggg
//
LOCUS       DASPYY010000345         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1043285, whole genome shotgun sequence.
ACCESSION   DASPYY010000345 DASPYY010000000
VERSION     DASPYY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043285"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VEP73_05300"
     CDS             <1..415
                     /locus_tag="VEP73_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HYT25882.1"
                     /translation="PAMVAAAARHELGSAAASTIRPPGFDRLPARNRARGHLLGRQLG
                     GSGDLPANLVALYQARANTPVMRDYETAVAEAVRAGETIRYTVRPGYPGPTANGAPST
                     IRITAAGNHGFRLDVTIANTPEAAVSETIPPPATP"
     gene            434..967
                     /locus_tag="VEP73_05305"
     CDS             434..967
                     /locus_tag="VEP73_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HYT25883.1"
                     /translation="MLEQLRLLLPPPSDPVEPGRPEGWGQVEAALGTELPDDFKAFTE
                     LYGSGKVDDFLYLFNPFAGGEDGNLLSEKDRVLAAYAETRAKFPGRLPLPPYPEPGGV
                     LPLGRTDNGDELFWVTRGAPRDWPVALLESRAASQEVHRMPVTGFLAALAAGELATRV
                     LPDDVVHRPTHEFAPFG"
     gene            complement(1019..1537)
                     /locus_tag="VEP73_05310"
     CDS             complement(1019..1537)
                     /locus_tag="VEP73_05310"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYT25884.1"
                     /translation="MRRPMAAVGSAAFFVLAPGVVAGLVPWWLTGWRVRQPLPSRAWA
                     PLRVVGVALLAAGVVVLVQAFVRFVVEGLGTPAPVAPTRSLVVGGLYRYVRNPMYLAV
                     VATIVGQALALGQPALLLYAAAAGLAMSAFARWYEEPALARQFGAQYEAYRRAVPAWW
                     PRRHPWEPGQAD"
     gene            1594..2067
                     /locus_tag="VEP73_05315"
     CDS             1594..2067
                     /locus_tag="VEP73_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HYT25885.1"
                     /translation="MEEDDIGALDECALYVAPHEESRRRWLAGVGALARSWWLFLVGG
                     VLSILAGVIAFAWPGRTLLVVSVLLAWFLVFKGIVDAISALANTHRDWWWVTLLLGFL
                     EFLLGIWAVGYPGRALFVFVNLVGIYAILYGFTEIFAGFALRELTHGPRRRRPAV"
     gene            2261..>2771
                     /locus_tag="VEP73_05320"
     CDS             2261..>2771
                     /locus_tag="VEP73_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYT25886.1"
                     /translation="MSDEQFRLLVAAVKDYAIFLLDPDGQVVSWNAGAERIKGYAAEE
                     IVGQSFTRFYLPEDVAAGVPDRVLGRARTEGRFEGYGWRLRKDGSRFLASVTVTALRD
                     AGGVLQGFAKVTRDVTEQQRQQEELAASAARWRTTVEHLLDPLAIMSAVRDAEGQVVD
                     FRYDYVNPAH"
BASE COUNT          369 a         1043 c          997 g          362 t
ORIGIN      
        1 ccccgcgatg gtcgccgccg ccgcccgcca cgagctcggc tccgcggccg cctcgaccat
       61 ccggccgccc ggcttcgacc ggctccccgc gcgcaaccgc gcccgcgggc acctgctcgg
      121 ccgccagctc ggcggctccg gtgacctgcc ggccaacctg gtcgcgctct accaggcgcg
      181 ggcgaacacc ccggtgatgc gcgactacga gaccgccgtc gccgaggcgg tccgggccgg
      241 cgagacgatc cgctacaccg tccggccggg gtaccccggc cccacggcca acggggcgcc
      301 gagcacgatc cggatcaccg cggccggcaa ccacggcttc cgcctcgacg tcaccatcgc
      361 caacaccccc gaggccgccg tgagcgagac catccccccg ccagccacgc catagcccgc
      421 gaacggagcc acgatgctgg aacaactgcg cctgctcctg ccacccccgt ccgacccggt
      481 cgagccgggc cgccctgaag gctggggcca ggtcgaggcg gcgctcggga ccgagctgcc
      541 cgacgacttc aaggccttca ccgagctgta cggctccggc aaggtggacg acttcctgta
      601 cctgttcaac ccgttcgccg ggggggagga cggcaacctg ctgtccgaga aggaccgggt
      661 gctggccgcc tatgccgaga ccagggcgaa gttccccgga cggctgccgc tgccgcccta
      721 tcccgagccc ggcggggtgc tgccgctcgg gcgcaccgac aacggcgacg agctgttctg
      781 ggtgacgcgg ggcgcacctc gtgactggcc ggtcgcgctg ctcgagtcgc gcgccgccag
      841 ccaggaggtc caccgcatgc cggtgaccgg cttcctcgcc gcgctcgccg ccggcgagct
      901 tgccacccgg gtgcttcccg acgacgtggt ccaccgcccg acgcacgagt tcgccccctt
      961 cggttgaccg gcctgccggc ctgccggccc cgccgaacgg cacgtccccg caggcgggct
     1021 agtcggcctg gcccggttcc caggggtgcc ggcggggcca ccaggccggc acggcccgcc
     1081 ggtaggcctc gtactgcgcg ccgaactggc gggccagggc cggctcctcg taccagcggg
     1141 cgaacgcgct catggcgagg ccggcggcgg cggcgtacag cagcagggcg ggctggccca
     1201 gcgccagcgc ctgcccgacg atggtcgcca ccaccgccag gtacatgggg ttgcggacgt
     1261 accggtagag cccgccgacg acgagcgacc gggtcggcgc gaccggcgcg ggggtaccga
     1321 ggccctccac gacgaagcgc acgaacgcct gcaccagcac gaccacgccc gcggcgagca
     1381 gggccacccc caccacccgc agcggcgccc aagcccggga cggcaggggc tgtcgcacgc
     1441 gccagccggt cagccaccac gggaccaggc ccgcgacgac gcccggtgcc agcacgaaga
     1501 acgccgcgct gccaaccgcg gccatcggtc tgcgcatcct gccaccctcc tgcgtcgatg
     1561 ccggcgagcc cggtcggcgg tccattggcc cgcgtggagg aagacgatat cggcgcgctg
     1621 gacgagtgcg ccctgtacgt cgcgccccat gaggaatctc gccgacgatg gctggcgggc
     1681 gtcggcgctc tggccaggag ctggtggctg ttcttggtcg gcggcgtcct gtcgatcctc
     1741 gccggggtca tcgccttcgc ctggccggga cggaccctgc tcgtcgtgtc ggtgctgctg
     1801 gcctggttcc tggtcttcaa gggcatcgtc gacgcgatca gcgcgctggc caacacccac
     1861 cgggactggt ggtgggtgac gctgctgctc ggcttcctgg agttcctgct cggcatctgg
     1921 gccgtgggct acccgggccg ggcgctgttc gtgttcgtca acctcgtcgg catctacgcg
     1981 atcctctacg gcttcaccga gatcttcgcc gggttcgcgc tccgcgagct cacccacggc
     2041 cctcgccgtc gccggcctgc ggtgtgaggc cgattcgtcc ctgaccgcgg atctggcgga
     2101 cgagccggtc ggcgtcgcgc cagacgcgca ggacgcctcg gccaccgcgt cggcggcatg
     2161 ctcgttgaca aggggacgtg gacggtgaag tgtgcgggac gtgcacgccg accaactacc
     2221 tgagcctcga gccgggcgcg ccgcgggccg cccgcggcag gtgagcgacg agcagttccg
     2281 gctgctcgtc gcggcggtca aggactacgc gatcttcctg ctcgacccgg acggtcaggt
     2341 ggtgagctgg aacgccggcg ccgagcggat caaagggtat gcggccgagg agatcgtggg
     2401 gcagtcgttc acccgcttct acctgcccga ggacgtcgcc gccggggtgc ccgaccgggt
     2461 gctcggccgg gcgaggaccg agggccggtt cgaggggtac gggtggcggt tgcgcaagga
     2521 cggcagccgt ttcctggcgt ccgtgacggt cacggcgctg cgcgatgccg gcggcgtgct
     2581 gcagggcttc gccaaggtga cccgggacgt gaccgagcag caacgccagc aggaggagct
     2641 ggccgcctcg gcggcacggt ggcggaccac ggtggagcat ctgctggacc ccctggccat
     2701 catgtcggcg gtgcgggacg ccgaggggca ggtggtcgac ttccgctacg actacgtcaa
     2761 cccggcccac g
//
LOCUS       DASPYY010000346         1807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2448453, whole genome shotgun sequence.
ACCESSION   DASPYY010000346 DASPYY010000000
VERSION     DASPYY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448453"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VEP73_05325"
     CDS             <1..656
                     /locus_tag="VEP73_05325"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYT25887.1"
                     /translation="LSRIVDYTDRNTCVLFGDGAGAAVLRPCGPEDGILAVSIHSDGV
                     LGDLLEVPAGGSLRPASAETVRDRDHFIKMRGRELFKVAVRGMEDSLRRSLDRAGLSV
                     GDLDLVIPHQANQRIIDATRERLGVPADKVILNIARFGNTSSASIPISLDEVQRQGRL
                     KPGDHVAFVAFGGGATWGATVSRWTMPAPQRSQPAAAPGAEAEPQAAVPSPVRGTGS"
     gene            653..1624
                     /gene="fabD"
                     /locus_tag="VEP73_05330"
     CDS             653..1624
                     /gene="fabD"
                     /locus_tag="VEP73_05330"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYT25888.1"
                     /translation="MKLAFLFPGQGSQGVGMGKALAEQFPAAREAFVIADRVLGFPLS
                     RVCWEGPDEELRATQHAQPALLAHSVAALRLLEAHGFRPAWVAGHSLGEYSACVAAGA
                     VSYEDALRLVRRRGELMQEAGRRRPGTMAAILGLGAPEVEAACAAASGAGLEVVAAND
                     NAPGQIVISGDVEAVKRACEIAKARGARRAIGLPVSGAFHSPLMRPAADQLAGVLDAV
                     ELHDPRCPIVSNVDAREVRTAGEVRAALKRQLTEPVRWHESMRLLMGRKPDDFVEVGT
                     GRVLCGLLRSIDPAVAPIALNLGEPKDVEAIVSRLESHRQPAAKGGD"
     gene            1626..>1807
                     /locus_tag="VEP73_05335"
     CDS             1626..>1807
                     /locus_tag="VEP73_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT25889.1"
                     /translation="MEQTVGIPQAAPTAELAGQAAFVTGGATGIGLAVVRALAGRGAS
                     VACFGRNFERAESVAGS"
BASE COUNT          228 a          615 c          728 g          236 t
ORIGIN      
        1 tgctgtcgcg gatcgtggac tacaccgacc ggaacacctg cgtcctgttc ggcgacggcg
       61 cgggtgcggc ggtcctgagg ccctgcggcc ccgaggacgg gatcctcgcg gtctcgatcc
      121 acagcgacgg cgtgctcggc gacctgctcg aagtgccggc cggcggctcg ctcaggcccg
      181 ccagcgccga gacggtgcgc gaccgcgacc acttcatcaa gatgcggggc cgcgagctgt
      241 tcaaggtcgc agtgcgcggc atggaggaca gcttgcggcg gtcgctcgat cgggcggggc
      301 tctcggttgg cgacctggac ctcgtcatcc cgcaccaggc gaaccagcgc atcatcgacg
      361 cgacgcgcga gcgtctcggc gtgcccgcgg acaaggtgat cctcaacatc gcgcgcttcg
      421 gcaacacctc ctcggcctcg atcccgatca gcctggacga ggtgcagcgg caggggaggc
      481 tcaagccggg cgaccacgtc gctttcgtgg ccttcggggg cggggcgacc tggggggcga
      541 cggtctcgcg ctggaccatg ccggcgccgc aacgctccca gccggcggcg gcgccgggcg
      601 ccgaggccga gccccaggcg gccgtgccga gcccggtgcg ggggaccggc tcgtgaagct
      661 cgcgttcctg ttccccggcc agggatcgca gggggtgggg atgggcaagg cgctggcgga
      721 gcagttcccg gccgcgcggg aggcgttcgt gatcgccgac cgcgtgctcg gcttcccgct
      781 cagccgggtg tgctgggagg gccccgacga ggagctgcgc gccacgcagc acgcgcagcc
      841 cgcgctgctc gcgcacagcg tcgccgcgct gcgcctgctc gaggcgcacg gtttccggcc
      901 ggcctgggtg gcgggccaca gcctgggcga gtattcggcc tgcgtggcgg ccggggcggt
      961 gtcctacgag gacgcgctgc gcctggtgcg gcgtcggggc gagctcatgc aggaggccgg
     1021 gcggcggcgt cccggcacga tggcggcgat cctggggctc ggggcgccgg aggtcgaagc
     1081 ggcgtgcgcg gcggcgagcg gggcggggct cgaggtggtg gccgccaacg acaacgcgcc
     1141 gggccagatc gtgatctcgg gcgacgtcga ggcggtcaag cgggcgtgcg agatcgcgaa
     1201 ggccaggggc gcccggcggg cgatcgggct cccggtgagc ggcgcgttcc attcaccgct
     1261 catgcgaccg gcggcggacc agctcgcggg ggtactcgac gccgtggagc tccacgatcc
     1321 gcgttgcccg atcgtctcga acgtcgacgc ccgggaggtc cggaccgccg gcgaggtgcg
     1381 ggcggcgctc aagcggcaat tgaccgagcc cgtccggtgg catgagtcga tgaggttgct
     1441 gatgggtcga aagcccgacg acttcgtcga ggtcggaacc ggccgcgtgc tgtgcggcct
     1501 gctccggagc atcgatcccg cggtggcgcc gatcgccctc aacctggggg agccgaagga
     1561 cgtcgaggcg atcgtgagcc ggctcgagtc ccaccgccag ccggccgcga aaggcggcga
     1621 ctgagatgga gcagaccgtg gggatcccgc aggcggcgcc aaccgccgag ctcgccgggc
     1681 aggcggcatt cgtgaccggc ggggcgaccg ggatcggcct cgcggtggtg cgcgcgctcg
     1741 ccgggcgggg ggcttcggtc gcatgcttcg gccgcaactt cgagcgcgcc gagtcggtcg
     1801 ccggctc
//
LOCUS       DASPYY010000347         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1185335, whole genome shotgun sequence.
ACCESSION   DASPYY010000347 DASPYY010000000
VERSION     DASPYY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185335"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VEP73_05340"
     CDS             complement(<1..244)
                     /locus_tag="VEP73_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25890.1"
                     /translation="MTMAAGRAARPRPNPEPQLEPARRLRAVAPLRRVPRLPFLLLTL
                     GAILAAVLGLVSLNVNVNQQAFELARLARENRAAEQR"
     gene            complement(241..1260)
                     /gene="rsmH"
                     /locus_tag="VEP73_05345"
     CDS             complement(241..1260)
                     /gene="rsmH"
                     /locus_tag="VEP73_05345"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYT25891.1"
                     /translation="MAVTASAHTPVMLDRSVALLRPRPGGVYVDATLGLGGHAEAVLA
                     ASAPDGLVIGLDRDPAALAAARGRLAADGGRLVAVPASFADLRRVVEGVGVDAVDGIL
                     YDLGVSSLQLDEADRGFSYRADAPLDMRMDPTGGPTAADVVNTYPQAELARVLREYGE
                     ERFAGRIARFVADARRRAPVRTTAELVELVKAAVPAAARRTGPHPARRTFQALRIEVN
                     GELAALSASLPQAIDLLAPGGRIVVLAYHSLEDRIVKRALAAAAAGDYSPPELPEHVV
                     RRTPVLRILTRRPETPPAGEVAANPRAESAKLRAAEKLAAPGATAAGDPHAGTSSTEF
                     PEGAA"
     gene            complement(1260..1697)
                     /gene="mraZ"
                     /locus_tag="VEP73_05350"
     CDS             complement(1260..1697)
                     /gene="mraZ"
                     /locus_tag="VEP73_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYT25892.1"
                     /translation="MFIGTYDHSLDPKGRVILPRKFRDELGQDMVFTKGIERCLYVFP
                     LAEFEAFAVKLRTLPLTERPSRDFVRVFVAGASQESADAQGRVVIPQPLREYAGLAKD
                     VVVVGQLSRIEIWDRSEWERYIPAAQASYSEETNTAHLAELGI"
     gene            complement(1906..>3455)
                     /locus_tag="VEP73_05355"
     CDS             complement(1906..>3455)
                     /locus_tag="VEP73_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYT25893.1"
                     /translation="RGPAAAAGRALSPWVAPLALGALGALLLITLLGREDARVGPATV
                     RLSVQPALHGSSRLAVPPFGSVSARTHDGLVQFRATLDDVDVPALREELVDDAPAGGL
                     RHPTLAATLLPLEQEARRAARQFLLRLALLGLAGGAAATLAFARRTRRRLLRCGLGGV
                     LATAALLVPALATYDVGAFRAPRYEGALEYAPALIGDAQTGLDRLRTLREEMVRIGEN
                     LNRAYDALGKPVPQVDGATVRVLHIADVHLNPAGFDLAQQLAAKFDVAAVVDTGDMGT
                     WGLPPERAVTRRVREFKLPYLFVKGNHDNDAMVRAVAANPNARVLDSSATEVAGIRFY
                     GVADPVFSPGQGYRVPIFERLKEQRSVAVGAALDAQAAGEGPRLPDVLLVHDPKLATY
                     AVGHVATVLDGHLHAFGTSVANGTRMLTTGTVGGAGPDGLRSRRPLPYTAEVLYFDPV
                     TRRPVAVDRITVGPFESSFSVDRELTPEGGTAFVPDPVEVPAGLLPAPPSGEVEAGPA
                     HPRAQLG"
BASE COUNT          428 a         1208 c         1417 g          402 t
ORIGIN      
        1 agcgctgctc ggccgcgcgg ttctcgcgcg ccaggcgggc cagctcgaac gcctgctggt
       61 tgacgttgac gttgagcgac accaggccga gcacggcggc caggatggcg cccagggtga
      121 ggagcaggaa gggcagcctc gggacgcgcc ggagcggcgc gaccgcgcgg agccggcgcg
      181 ccggctcgag ttgcggctcg gggttggggc gcggcctcgc cgcgcggcct gcggccatgg
      241 tcatgctgcc ccctctggga attcggtgct ggacgtcccg gcgtgggggt cgccggctgc
      301 tgtggcgccg ggtgcggcga gcttctcggc cgcgcgcagc ttggcgctct ccgcgcgcgg
      361 gttggcggcc acctcgccgg ccggcggggt ctccgggcgg cgggtgagga tgcggaggac
      421 cggcgtgcgg cggaccacgt gctcgggcag ctcgggcggc gagtagtcgc cggccgcggc
      481 ggcggccagc gcccgcttca cgatgcggtc ctcgagcgag tggtaggcga gcacgacgat
      541 gcggccgccg ggggcgagga ggtcgatcgc ctgcggcagc gaggcgctga gggcggcgag
      601 ctcgccgttg acctcgatcc gcagcgcctg gaaggtacgg cgggcggggt gcgggccggt
      661 gcgcctggcg gcggcgggca cggccgcctt gaccagctcg acgagctcgg cggtggtgcg
      721 gaccggggcg cggcggcggg cgtcggcgac gaaccgggcg atgcggcccg cgaaccgctc
      781 ctcgccgtac tcgcgcagga ccctcgccag ctcggcctgc gggtaggtgt tcaccacgtc
      841 ggccgcggtg gggccgccgg tcgggtccat gcgcatgtcg aggggcgcgt ccgcgcggta
      901 gctgaagcca cggtcggcct cgtcgagctg cagcgacgag acgccgaggt cgtagaggat
      961 cccgtcgacg gcgtcgacgc cgacgccctc gaccacccgg cggaggtcgg cgaacgaggc
     1021 cgggacggcg accagccggc cgccgtcggc ggcgagccgc ccccgggccg cggcgagcgc
     1081 ggccgggtcg cggtccaggc cgatcaccag gccgtcgggc gcgctcgcgg cgagcacggc
     1141 ctcggcgtgg ccgccgaggc cgagggtggc gtccacgtac acgccacccg ggcggggccg
     1201 gagcagcgcg accgaccggt cgagcatgac cggggtgtgg gccgatgccg tcaccgccat
     1261 cagattccca gctcggcgag gtgggcggtg ttggtctcct cggagtagga cgcctgggcc
     1321 gccgggatgt accgctccca ctcgctccgg tcccagatct cgatgcggga gagctggccg
     1381 acgacgacca cgtccttggc cagccccgcg tactcccgga gcggctgcgg gatgacgacg
     1441 cggccctggg cgtcggcgct ctcctggctc gcgccggcca cgaacacgcg cacgaagtcc
     1501 cggctcgggc gctcggtcag gggcagggtg cgcagcttga ccgcgaaggc ctcgaactcg
     1561 gccagcggga agacgtagag gcagcgctcg atgcccttgg tgaagaccat gtcctgcccc
     1621 agctcgtcgc ggaacttgcg ggggaggatc acccggcctt tgggatcgag ggagtggtcg
     1681 taggtgccaa tgaacacccc ggccgtcccc ctcgtcgctg cagttttcct ccactggccg
     1741 ccactttact ccaccgccta tccaccgtca acgcggctcg aggctgcccg acggcctgaa
     1801 ccaggccgct gagcagggaa aacgcagcag aggccatggt gtgcctcgag ctcagcccgg
     1861 gtccgcgccc ctccacctcg ctccacctcg ggaggggccg gagcgtcagc cgagctgcgc
     1921 ccgcgggtgc gccggcccgg cctcgacctc gccggacggc ggggccggga gcaggccggc
     1981 cgggacctcg acggggtcgg ggacgaaggc cgtcccgccc tcgggggtca gctcgcggtc
     2041 gaccgagaac gacgactcga aggggcccac ggtgatgcgg tcgaccgcga cgggccggcg
     2101 ggtcaccggg tcgaagtaga ggacctcggc ggtgtagggc agcggccgtc gggaccgcag
     2161 gccgtcggga ccggcgcccc ccaccgtccc ggtggtgagc atccgcgtgc cgttcgcgac
     2221 cgacgtgccg aaggcgtgca ggtgcccgtc gagcacggtg gcgacgtggc cgacggcgta
     2281 ggtggccagc ttggggtcgt ggacgaggag gacgtcgggg agccgggggc cctcccccgc
     2341 cgcctgcgcg tcgagcgcgg cgccgacggc gacgctgcgc tgctccttca ggcgctcgaa
     2401 gatcggcacc ctgtacccct gccctggcga gaacaccggg tcggcgaccc cgtagaagcg
     2461 gatgcccgcg acctcggtgg ccgacgagtc gagcacgcgc gcgttggggt tggcggccac
     2521 cgcgcgcacc atggcgtcgt tgtcgtggtt gcccttgacg aacaggtagg ggagcttgaa
     2581 ctcgcggacc cggcgggtga cggcccgctc gggcggcagg ccccaggtgc ccatgtcgcc
     2641 ggtgtcgacg accgcggcca cgtcgaactt cgccgcgagc tgctgggcca ggtcgaagcc
     2701 ggccgggttg aggtgcacgt cggcgatgtg gagcacccgc acggtggccc cgtcgacctg
     2761 cggcaccggc ttgccgagcg cgtcgtaggc ccggttcagg ttctcgccga tccggaccat
     2821 ctcctcgcgg agggtgcgga gccggtcgag tcccgtctgg gcgtcgccga tgagcgcggg
     2881 ggcgtactcg agcgcgccct cgtagcgggg cgcccggaag gcgccgacgt cgtaggtggc
     2941 gagcgccggg acgagcagcg ccgcggtggc caggacgccg ccgaggccgc agcgcagcag
     3001 ccggcggcgg gtgcggcggg cgaacgccag cgtcgcggcg gccccgccgg cgagcccgag
     3061 gagggcgagg cgcaggagga actggcgggc ggcccgcctc gcctcctgct cgagcgggag
     3121 cagggtggcg gccagcgtcg gatggcgcag gccgccggcc ggggcgtcgt cgaccagctc
     3181 ctcccgcagg gcggggacgt cgacgtcgtc gagggtggcg cggaactgga cgaggccgtc
     3241 gtgggtccgc gcggacacgg acccgaacgg cgggacggcg agccggctcg agccgtgcag
     3301 ggcgggctgg accgacaggc gcacggtggc cggcccgacg cgggcgtcct cccggccgag
     3361 cagggtgatg agcagcaggg cgccaagcgc gccgagggcg agcggcgcca cccagggcga
     3421 gagcgcccgc cccgcggcgg cggcgggccc gcggg
//
LOCUS       DASPYY010000348         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1887883, whole genome shotgun sequence.
ACCESSION   DASPYY010000348 DASPYY010000000
VERSION     DASPYY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887883"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /gene="pglZ"
                     /locus_tag="VEP73_05360"
     CDS             complement(<1..787)
                     /gene="pglZ"
                     /locus_tag="VEP73_05360"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HYT25894.1"
                     /translation="MPAPVLLVRAEPVWEGRPVFEAGDRTVRVVACVSPLAVLEQVTG
                     HVRGEDGSVLVVLTDREEGELGNGVLSHALRHQVLTVEPWSLVEESFGAQRLDPRLAA
                     ERWAAEALIDAMPPGGWPPLSGTVLNRDVALRHLAARRLGLDRLGLAPDDLDAQALLR
                     WSALPGAVESFGRLRAEERAGLVAWLTERNGQVARTLFALVHAGHGADALAVGLVSGA
                     LWAPAARGATERAQGRVDRYVGDAHLDDGTIRAFADVAEEVVGG"
     gene            complement(889..>2599)
                     /locus_tag="VEP73_05365"
     CDS             complement(889..>2599)
                     /locus_tag="VEP73_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HYT25895.1"
                     /translation="DDAFQPLDPTSWRIIVDHPFDEGGHGPADDRQRVQTLRSNGMQA
                     RTVCWLPASLTTERLGDLRRLVIIDAVLGGQRFDSHAQHLNPTDRHRARATLESQRDA
                     LLTKVRGVLRQAYGLATKQPGDVVTSYDDHLLSLTPGLRPTLPVGAAMRDALRSVADQ
                     MLAQQYPAHPDFDPDRTGATVRPADARVVLEYVRRAVEWPDGRIEVEKRDRATMRRIA
                     NPLQLGEMHEAAFVLGRYWVQHFHQKAAQEGIDGDLKVADLARWLDDPDPRGLEKLLA
                     NLVIACFAEQTDRAWVRHGGVLEPPPELTAITHDLALREQPLPSEEAWEAARTRAMDV
                     FGINPPALRRGRVVAMFVRQLSEHARSYRDAAHTLVQRLARHAEPLGLDPSATEGRLH
                     TARAAADLLDGLGSRQHSPEIVNHLARSDLGGPAQRTGRSIRSAQQVADALARASWDT
                     FGLIAGLGELYAAEAATIFEALRRAAQADELTASLPDALDKARVEATALLRKATSRPP
                     GPPAARVPQPPVPGQVRTEPDTAGGMQQIIAADLETALAALRAFVQAHPEAMVEVTWR
                     VVE"
BASE COUNT          300 a          981 c          970 g          348 t
ORIGIN      
        1 gcccgccgac gacctcctcg gccacgtcgg cgaacgcccg gatcgtgccg tcgtcgaggt
       61 gcgcgtcgcc gacgtagcgg tccaccctgc cctgcgcccg ttcggtggca ccccgggcgg
      121 ccggcgccca gagcgcaccg gacacgagcc cgaccgccag cgcgtcggcg ccgtgcccgg
      181 cgtggacgag ggcgaacagc gtcctggcga cctggccgtt ccgctcggtc agccacgcca
      241 ccaggcccgc ccgctcctcg gcgcgcagcc tgccgaacga ctcgaccgcg ccgggcagcg
      301 cgctccaccg cagcagcgcc tgggcgtcga ggtcgtccgg tgccagtccc agccggtcca
      361 ggccgagccg ccgggcggcc aggtggcgca gcgcgacgtc ccggttgagc acggtgcccg
      421 acagcggcgg ccagccgcct ggcggcatcg cgtcgatcag cgcctcggcc gcccaccgct
      481 cggccgccag ccgggggtcg agccgctgcg cgccgaagct ctcctccacg agcgaccacg
      541 gctccacggt gagcacctgg tgccgcagcg cgtggctgag cacgccgttg cccagctcgc
      601 cctcctcccg gtcggtgagc acgacgagca ccgagccgtc ctcaccgcgg acgtggcccg
      661 tgacctgctc caggacggcg agcggggaga cgcaggcgac cacccggacc gtccggtcgc
      721 cggcctcgaa gacgggcctc ccctcccaga ccggctccgc ccggaccagc aggaccggtg
      781 cgggcaaggt gccttccctg ccgcggtgac gctggagcca cctggtcacc tgttgctcca
      841 gggccgcggg cttgatccgc agggccctgg gcaaggcgac ggcagcggtc actcgacgac
      901 ccgccaggtg acctcgacca tcgcctcggg atgggcctgg acgaaggctc gcagtgccgc
      961 cagcgccgtc tccaggtcgg ccgcgatgat ctgttgcatt ccgccggcgg tgtcgggctc
     1021 ggtcctgacc tggcccggca ccggtggctg cggcacccgc gctgcgggtg ggccgggcgg
     1081 ccggctggtc gccttccgca gcagcgcggt cgcctcgacc cgggccttgt cgagcgcgtc
     1141 cggcagcgag gcggtcagct cgtcggcctg ggccgcacgc cgcagcgctt cgaagatggt
     1201 cgccgcctcg gccgcgtaca gctcgccgag gccggcgatc aggccgaagg tgtcccacga
     1261 cgctcgggcc agggcgtcgg cgacctgctg ggcgctccgg atgctcctgc ccgtccgctg
     1321 cgccggcccg cccaggtcgg agcgggccag gtggttgacg atctccgggc tgtgctggcg
     1381 cgagccgagc ccgtcgagca ggtcggcggc ggcccgggcc gtgtggagcc gcccctcggt
     1441 cgcggacggg tcgaggccga gcggctcggc atgccgcgca aggcgctgca ccagggtgtg
     1501 cgcggcgtcc cggtagctgc gggcgtgctc cgagagctgg cggacgaaca tggcgaccac
     1561 ccggccccgg cgcagcgccg gcgggttgat gccgaagacg tccatcgccc gtgtcctggc
     1621 cgcctcccag gcttcctcgc tcggcagggg ctgctcgcgc agcgccaggt catgggtgat
     1681 ggcggtcagc tcgggcggcg gttcgagcac gcctccatgg cggacccagg cccggtcggt
     1741 ctgctccgcg aagcaggcga tcaccaggtt ggcgagcagc ttctccagcc cgcgtgggtc
     1801 ggggtcgtcc agccacctgg cgaggtcggc caccttcagg tcgccgtcga tcccttcctg
     1861 ggcggccttc tggtggaagt gctgcaccca gtaccggccg aggacgaacg ccgcctcgtg
     1921 catctcgccc agctgcagcg ggttggcgat ccgccgcatc gtggcgcggt cccgcttctc
     1981 cacctcgatc cggccgtcgg gccattcgac cgccctgcgg acgtactcga gcaccaccct
     2041 ggcgtccgcg ggacggaccg tggccccggt gcggtcgggg tcgaagtccg ggtgcgccgg
     2101 gtactgctgg gccagcatct ggtcggcgac gctgcgcagc gcgtcgcgca tcgccgcgcc
     2161 caccggcagc gtcggccgca gccccggcgt gagggagagc aggtggtcgt cgtacgaggt
     2221 gacgacgtcg ccgggctgct tggtggccag cccgtacgcc tgtcgtaaca cgccgcggac
     2281 cttggtgagc agcgcgtccc gctggctctc cagggtggcg cgggcgcggt gccggtcggt
     2341 cgggttgagg tgctgggcgt ggctgtcgaa ccgctggccg cccaacaccg cgtcgatgat
     2401 caccagcctg cgcaggtcgc ccagccgctc ggtggtgagc gacgccggca gccagcagac
     2461 ggtgcgcgcc tgcatcccgt tcgagcgcag cgtctgcacc cgctggcggt cgtcggccgg
     2521 gccgtggccg ccctcgtcga agggatggtc gacgatgatc cgccacgagg tcgggtcgag
     2581 cggctggaag gcgtcgtcg
//
LOCUS       DASPYY010000349         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2729582, whole genome shotgun sequence.
ACCESSION   DASPYY010000349 DASPYY010000000
VERSION     DASPYY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729582"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VEP73_05370"
     CDS             <1..1673
                     /locus_tag="VEP73_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25896.1"
                     /translation="NPDRKAVTRDGDLLGAYWAQGGGARPQSLFEMRAVADEAAAGLA
                     EAELRCDEAARQLAVAAEAEEDARQALAQAQARRHEVDAAAAEVSGRLGRLAGAARAA
                     REEAARLDAAITSARQAAEKDLARLEQLRAELAEAEELDDTDEPVEPEHQTAREDLAE
                     RCTAARNAEMEARLEVRTAEERLRAIGGRAESLAAAAAAERATRLRAAERRQRRAAQA
                     AVARAVALGARYAVEAAEQSVNQALARRAGAEAASSGGAAELKAVRARVRDLAADLDT
                     VVNTAHGTEIARAEHRLRLAQLAEHALEEFGVEADALVAEYGPEVPVPVIGDDEALRS
                     TFERAAAERRAADAQRQLDRLGKVNPLALEEFEALTERHTFLATQLDDLRKTRRDLLT
                     VVKEVDDRVQEVFASAYADTAREFEHLFSRLFPGGQGNLVLTEPDDMLATGIEIEARP
                     PGKKVKRLSLLSGGERSLTAIAYLFAIFMARPSPFYVLDEVEAALDDTNLQRLLLVFE
                     ELRESSQLIVITHQRRTMEAADALYGITMRGDGVSTVISQQIATRQPA"
BASE COUNT          238 a          645 c          648 g          175 t
ORIGIN      
        1 aaaatcctga tcgtaaggcc gtgaccaggg acggtgacct gctcggggcc tactgggcgc
       61 agggcggcgg cgcccggccg cagagcctgt tcgagatgcg ggccgtggcc gacgaggcgg
      121 ccgccggcct ggccgaggcc gagctgcggt gcgacgaggc ggcccgccag ctggcggtgg
      181 cggccgaggc cgaggaggac gcgcggcagg ccctggcgca ggcgcaggct cgcaggcacg
      241 aggtcgacgc ggccgcggcc gaggtctccg gccggctcgg ccggctggcc ggggcggccc
      301 gggccgcgcg cgaggaggcc gcgcggctcg acgccgccat cacctcggcc cggcaggcgg
      361 ccgagaagga cctggcccgg ctggagcagc tccgggccga gctcgccgag gccgaggagc
      421 tggacgatac cgacgagccg gtcgagccgg agcatcagac ggcccgcgag gacctcgccg
      481 aacgctgcac cgcggcgcgc aacgccgaga tggaagccag gctcgaggtc aggaccgcgg
      541 aggagcggct gcgggcgatc ggcggccggg ccgagtccct ggccgccgcc gcggccgccg
      601 agcgcgccac ccgcctgcgc gcggccgagc gccgccagcg gcgggccgcc caggccgccg
      661 tggcccgggc cgtcgcgctg ggcgcccgct acgcggtcga ggcggccgag cagtcggtga
      721 accaggccct ggcccggcgg gccggggccg aggcggccag cagcggcggc gccgccgaac
      781 tgaaggcggt ccgcgcccgg gtccgcgacc tggccgccga cctggacacc gtggtgaaca
      841 ccgcgcacgg caccgagatc gcccgggccg agcaccggct gcgactggcg cagctggccg
      901 agcacgcgct ggaggagttc ggcgtcgagg cggacgccct ggtggccgag tacggcccgg
      961 aggtgcccgt gccggtcatc ggcgacgacg aggccctgcg gtcgacgttc gagcgcgccg
     1021 cggccgagcg acgggccgcc gacgcgcagc gccagctgga ccggctgggc aaggtcaacc
     1081 cgctggccct ggaggagttc gaggcgctca ccgagcggca caccttcctg gccacccagc
     1141 tcgatgacct ccgcaagacc cggcgcgacc tgctcaccgt ggtcaaggag gtcgacgacc
     1201 gggtacagga ggtgttcgcg tccgcctacg ccgacaccgc gcgcgagttc gagcacctgt
     1261 tcagccggct gttccccggc ggccagggca acctcgtgct gaccgaaccc gacgacatgc
     1321 tcgccaccgg catcgagatc gaggcgcggc cgccgggcaa gaaggtcaag cggctgtcgc
     1381 tgctgtccgg cggggagcgc tcgctgaccg ccatcgccta cctgttcgcg atcttcatgg
     1441 cccgcccctc gccgttctac gtgctcgacg aggtcgaggc ggccctggac gacaccaacc
     1501 tgcagcggct gctcctggtc ttcgaggagc tcagggagag ttcccagctc atcgtcatca
     1561 cccaccagcg ccgcacgatg gaggccgcgg acgccctcta cggcatcacc atgcgcggcg
     1621 acggcgtctc caccgtcatc agccagcaga tcgccacccg ccagcccgcc tagcccggca
     1681 cctcgcccgc tccgggcacc tcgccc
//
LOCUS       DASPYY010000350         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1185636, whole genome shotgun sequence.
ACCESSION   DASPYY010000350 DASPYY010000000
VERSION     DASPYY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185636"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VEP73_05375"
     CDS             <1..802
                     /locus_tag="VEP73_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYT25897.1"
                     /translation="ALLALGGRRARKEPTPDHPFGYGRERYFWAFLVAVVLFTVGSLF
                     ALYEGYEKLRNPHGLESPVWAFGTLAVAVVLEALSLRTAVREANKHRGRQPWWKFIHR
                     AKSPEIPVVLLEDTGALLGLAFALVGVTLTVVTGNPRFDAVGSLAIGLLLGVIAVVVG
                     TEMRSLLVGESAGRAEVNAIREALESSPGMRRVIHLRTMHLGPEELLVGAKVELDLEL
                     SFADVAEAIDAAEAAIRERVPSARIIYLEPDVERDRAGEPVPPPTPTT"
     gene            complement(868..1839)
                     /locus_tag="VEP73_05380"
     CDS             complement(868..1839)
                     /locus_tag="VEP73_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25898.1"
                     /translation="MLRFVGAIAVASMLLPQAGANGAGRSTDVPTTLKQLKAALADAN
                     ANEAKLTKAVAQADDALNRAEHDLADAGDQRLAAHLRSIYAQKAVNDAARRVQGLRSI
                     LADRARGIYISGSPADIRALVQSGDPEALLDQVAILDHLAQQGNDSLADLLVAQRDYA
                     RARVVLLQAEQDATRAEAAIRVKIKQATQFRDVRLQALSKLTAKITLLRGRVAATQVS
                     QRIGDTQLAGVVRRSNTRCDLSGTSNGEYNIIMRESGGNPYADNPTSTAFGLGQLLYD
                     GRVHYLGVANANTTDCGLQLQAFRGYVRDRYGTAANAWAFWQAHHWY"
     gene            2017..2910
                     /locus_tag="VEP73_05385"
     CDS             2017..2910
                     /locus_tag="VEP73_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HYT25899.1"
                     /translation="MDDTRPTRIVLDSPSLVYRAFFALPTSIADPDGVPVNAVRGYLD
                     MLSRLLVDHRPLAVLNVLDASWRPQWRVDAWPGYKATRRDDPPELPGQVPVLREVLEA
                     AGLPVAEAQDYEADDVIGTVAAAAGPDERLAVVTGDRDLLQVIRDPVAWVLFPVKGTS
                     QLTRFDEAAVAARYGVPPSRYVDYAILRGDPSDGLPGIPGVGEKTAARLVSEFASLDE
                     LVAARDRLPPRLAAALGASGEYLVAMRRIVPVATDVAWTATAAHPPDGARLDALAGRH
                     GLGGPVGRLMAALVTAGLAPA"
     gene            2984..3919
                     /locus_tag="VEP73_05390"
     CDS             2984..3919
                     /locus_tag="VEP73_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYT25900.1"
                     /translation="MAGLGPVRVGTCGWTDPTLIKAGTFYPAGANTAEERLRHYASQF
                     PVVEVDATYYALPWQEQAGLWVERTPPDFTFNVKAYSLLTQHPTMAKTIPADLRDSIL
                     PEHRGKQRVYPGHLGADAVEEIWRRFHEALLPLDSAGKLGAVLLQFPEWHVPSRGNRE
                     YLQLAREKLAGYRVCVEFRNRAWLGTDKDRDRTLGFLREHELALVCVDMPQGFRSSMP
                     PVAEVTNPALSVVRFHGRDPEAWEKKTVSERFRYLYDEDELREWVPRIEHLAEDAAEV
                     HAMMNNCYSDHAVVNARQLDALLTRSNASRTDTTS"
     gene            3954..>4142
                     /locus_tag="VEP73_05395"
     CDS             3954..>4142
                     /locus_tag="VEP73_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYT25901.1"
                     /translation="MHPDFALLGASWELALRADGYAEHTVRSYRRALASLAAHLEQAG
                     HGGVVPAELTRDQVRGWLV"
BASE COUNT          531 a         1469 c         1540 g          602 t
ORIGIN      
        1 ggcgctgctc gcgctcggcg gccggcgggc gcgcaaggag cccacgcccg accacccctt
       61 cgggtacggg cgggagcgct acttctgggc gttcctcgtc gcggtggtgc tgttcaccgt
      121 cggctcgctg ttcgccctct acgaggggta cgagaagctc aggaacccgc acgggctgga
      181 gtcgcccgtc tgggcgttcg gcaccctggc cgtcgccgtc gtcctcgagg cgctctcgct
      241 gcgtaccgcg gtcagggagg cgaacaagca ccggggccgc cagccgtggt ggaagttcat
      301 ccaccgggcc aagagccctg agatccccgt ggtcctgctc gaggacaccg gggcgctcct
      361 cggcctcgcc ttcgccctgg tcggggtgac cctcacggtc gtcaccggca acccccggtt
      421 cgacgccgtg ggcagcctcg cgatcggcct gctgctcggc gtcatcgccg tcgtcgtcgg
      481 caccgagatg cggagcctgc tggtcggcga gtcggccggg cgggccgagg tgaacgcgat
      541 ccgggaggcg ctcgagtcgt caccggggat gcgccgggtc atccacctgc gcaccatgca
      601 cctcggcccc gaggagctcc tcgtcggggc gaaggtcgag ctcgacctgg agctgtcgtt
      661 cgccgacgtc gccgaggcga tcgacgcggc ggaggcggcc atccgcgagc gggtgccctc
      721 cgcccggatc atctacctgg agcccgacgt cgagcgggac cgggcgggag agccggtccc
      781 gccgcccacc ccgaccacct gacccaccct gtggcgccgc ggcccgccgg atggggggcc
      841 gcggcgctcg ggacgtgacg cgggggctca gtaccagtgg tgggcctgcc agaacgccca
      901 cgcgttggcc gccgtgccgt agcggtcgcg gacgtacccg cggaaggcct ggagctgcag
      961 cccgcagtcg gtcgtgttgg cgttggcgac gccgaggtag tggacgcgac cgtcgtacag
     1021 gagctggccg agcccgaacg ccgtcgaggt ggggttgtcg gcgtaggggt tgccgcccga
     1081 ctcgcgcatg atgatgttgt actcgccgtt cgaggtgccg gacaggtcgc acctggtgtt
     1141 gctgcgtcgc accaccccgg cgagctgcgt gtcgccgatg cgctggctga cctgggtggc
     1201 ggccacgcgc ccgcgcagca gggtgatctt ggcggtcagc ttgctcagcg cctgcagccg
     1261 cacgtcgcgg aactgcgtgg cctgcttgat cttgacccgg atggccgctt cggcccgggt
     1321 cgcgtcctgc tcggcctgga gcaggaccac gcgtgccctg gcgtagtcgc gctgggcgac
     1381 gagcaggtcg gcgagggagt cgttgccctg ctgggcgagg tggtcgagga tcgcgacctg
     1441 gtcgagcagc gcctccgggt cgccgctctg cacgagcgcg cggatgtccg cgggcgagcc
     1501 cgagatgtag atgccgcggg cgcggtcggc gaggatcgac cgcagcccct ggacccgcct
     1561 tgccgcgtcg ttcacggcct tctgcgcgta gatgctgcgc aggtgcgccg cgagccgctg
     1621 gtcgccggcg tcggccaggt cgtgctcggc gcggttgagg gcgtcgtcgg cctgcgccac
     1681 ggccttggtc agcttggcct cgttggcgtt ggcgtcggcg agggcggcct tgagctgctt
     1741 gagggtggtc gggacgtccg tggaccgtcc agcaccgttg gctccggctt ggggaaggag
     1801 catcgacgcc accgcgatgg cgccgacgaa ccgaagcacc ctggttcgtc tcgtgggtgc
     1861 catgatgcga ctgcgctccc tgaagggggt tcgggaacat gcgttcgtgt cgagggggtc
     1921 acgaacgggg gaacgatatc gcaacgacct cgccgtttgg caaatcggcc ggccggccgc
     1981 ggcacgctcc ggtgccggac gtggcatgct ccggtgatgg acgacacccg gccgacccgc
     2041 atcgtgctcg actcgccgag cctcgtgtac cgggcgttct tcgccctgcc cacgtcgatc
     2101 gccgatccgg acggggtgcc cgtgaacgcg gtccggggct atctcgacat gctgtcgcgg
     2161 ctgctcgtcg accaccgccc gctcgccgtg ctgaacgtcc tcgacgccag ctggcgcccc
     2221 cagtggcggg tcgacgcctg gcccgggtac aaggcgaccc gccgcgacga cccgccagag
     2281 ctgcccggcc aggtgccggt cctccgggag gtgctcgagg cggccgggct gccggtcgcc
     2341 gaggcccagg actacgaggc ggacgacgtc atcggcaccg tcgccgcggc ggccggaccg
     2401 gacgagcggc tcgcggtcgt caccggcgac cgcgacctgc tccaggtcat ccgcgacccg
     2461 gtcgcctggg tgctgttccc ggtcaagggg acgtcccagc tcacgcgctt cgacgaggcg
     2521 gccgtcgccg ccaggtacgg cgtcccgccc agccgctacg tcgactacgc catcctccgc
     2581 ggtgacccgt ccgacggcct gcccgggatc ccgggcgtgg gggagaagac ggccgccagg
     2641 ctggtctcgg agttcgccag cctggacgag ctcgtggcgg cccgtgaccg gctgccgccg
     2701 cgcttggccg ccgcgctcgg tgcgagcggg gagtatctgg tcgcgatgcg gcggatcgtg
     2761 ccggtcgcga ccgatgtcgc ctggaccgcc accgcggccc acccgcccga cggcgcgcgc
     2821 ctggacgcgc tggccgggcg gcacggcctg gggggcccgg tcggccgcct catggcggcg
     2881 ctggtcaccg ccggcctcgc ccccgcgtag cgccggcagg cgcgcctgga cgcgctggcc
     2941 cgggcgcatg gcgcggccgc cgtgccggga ggacactggc ggcatggctg gcctcggacc
     3001 cgtgcgtgtc ggcacctgcg gctggaccga cccgaccctg atcaaggcgg ggaccttcta
     3061 cccggcgggg gcgaacaccg cggaggagcg gctgcgccac tacgcctcgc agttcccggt
     3121 ggtcgaggtc gacgcgacct actacgccct gccgtggcag gagcaggcgg ggctgtgggt
     3181 ggagcgcacc ccgcccgact tcaccttcaa cgtcaaggcc tacagcctgc tcacccagca
     3241 cccgaccatg gcgaagacga tcccggccga cctgcgcgac tcgatcctgc cggagcatcg
     3301 cggaaagcag cgggtctacc cgggccacct gggcgcggac gccgtcgagg agatctggcg
     3361 gcgattccac gaggcgctgc tgccgctgga ctcggccggc aagctcggcg ccgtgctcct
     3421 gcagtttccg gaatggcacg tacccagccg agggaacagg gagtatctcc agctcgcgag
     3481 ggagaagctc gccggctacc gggtgtgcgt ggagttccgg aaccgggcgt ggctcggcac
     3541 cgacaaggac cgcgaccgga cgctcgggtt cctgcgtgag cacgagctgg cgctggtctg
     3601 cgtggacatg ccgcaggggt tccggtcctc gatgccgccg gtcgcggagg tgaccaaccc
     3661 ggccttgtcg gtggtccgct tccacggccg cgacccggag gcgtgggaga agaagaccgt
     3721 gagcgagcgg ttccgctacc tctacgacga ggacgagctg cgcgagtggg tgcccaggat
     3781 cgagcacctg gccgaggacg ccgccgaggt ccacgccatg atgaacaact gctattccga
     3841 ccacgcggtg gtgaacgccc ggcagctcga cgcgctgctg accaggagta atgccagcag
     3901 aacggacaca acgagttgag gataccgccg tcctgacggc ataatcaacc cctatgcatc
     3961 ctgacttcgc cctgctcggc gcctcctggg agctcgccct ccgcgccgac ggctacgcgg
     4021 agcacaccgt ccgcagctac cggcgggcgc tggcgtcgct cgcggcccac ctcgagcagg
     4081 ccggccacgg tggggtggtg cccgccgagc tcacccgcga ccaggtccgc gggtggctcg
     4141 tg
//
LOCUS       DASPYY010000351         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2729881, whole genome shotgun sequence.
ACCESSION   DASPYY010000351 DASPYY010000000
VERSION     DASPYY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729881"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEP73_05400"
     CDS             <1..536
                     /locus_tag="VEP73_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HYT25902.1"
                     /translation="HAIALRCQLRIEPHLRRYGDAEAGRLVDLFGEPARWGDTLKPLQ
                     FANLSAMVQGFTGSTEVDLPVPCTYDFEVVSAKYLHSLDDGEVPLLLLFSGTVFRRGE
                     TGFSVEQVPWNKEATYRLPVRVWRETMDRYFPNSGWIRIRRDTLDALSRFKSREALPT
                     WDETLETLLKQAGEEAP"
     gene            533..1933
                     /locus_tag="VEP73_05405"
     CDS             533..1933
                     /locus_tag="VEP73_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25903.1"
                     /translation="MSFERARKVADAILFEGYVLYPYRASAQKNQARWQFGVLVPRAW
                     SEGGSGEAWSSQTECLVEPGDGAALEVRVRFLQLQARTVEEAVAGGGFRPVASLEVDG
                     APLAAWDEGVEREAGATLPVGELLAGERTVPVELPGGRESEPVRRASGELAGRVVRER
                     WPLSGRISVAAERLDGPYGVVRLRVRVENLTPWAAPGAGRDEAVRHSLAGAHSLLAVD
                     RGGFLSLLDPPEWAGPAAAACANVGTFPVLVGEPGQRDVVLSSPIILYDHPAVAPESP
                     GDLFDATEIDEILTLRTMALTDEEKREARGTDDRAAAIIDRVDAMPAEVLDRLHGAVR
                     YLRDATGPGGAHDRGAPVPGADGPPSSTPWWDPGADASVSPGTDSVRVSGVEVARGSL
                     VRLRPGRRRADAQDMFLEGRVATVEAVLHDVDEGRHLAVTLADDPAAAELHRWHGRFL
                     YFSPDEVEPLDAGVSR"
     gene            1930..2544
                     /locus_tag="VEP73_05410"
     CDS             1930..2544
                     /locus_tag="VEP73_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HYT25904.1"
                     /translation="MSGARKILVAGVGNIFLGDDGFGVEVARRLAGESPPGGVTVADF
                     GIRGVHLAYELLEGYDTTILVDAAPRGGEPGTVYVVEVDVDDPGAGIPSERAGEEGAL
                     VDAHGMEPQAVFGLLRALGAAAGRVLVVGCEPAEVTDRIGLSDPVARAVDEAVRVVGE
                     LVRDELGEASSGAWGGAGVGKARRGSGGSSSEAVAPGSLEGGGA"
     gene            2674..3015
                     /locus_tag="VEP73_05415"
     CDS             2674..3015
                     /locus_tag="VEP73_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HYT25905.1"
                     /translation="MHEVGLCESIVDAVERRAAGRRVRRVRVRVGVLHRVVEPALDQA
                     FSLVAGGTVAEGAAVDLVTVAARVACAACGHEAESDGLVVACPRCGATELELTGGDEL
                     VLESIQFEAPA"
     gene            3041..3313
                     /locus_tag="VEP73_05420"
     CDS             3041..3313
                     /locus_tag="VEP73_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HYT25906.1"
                     /translation="MCLGIPGEVVEVLHDRPDLAKVDVSGVRRAINIGLLEGETLEPG
                     DWILIHVGFALSKIDEEEAKAALDFLEGIGQAYADELAALQASRID"
     gene            3344..4414
                     /gene="hypD"
                     /locus_tag="VEP73_05425"
                     /pseudo
     CDS             3344..4414
                     /gene="hypD"
                     /locus_tag="VEP73_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
     gene            complement(4408..4560)
                     /locus_tag="VEP73_05430"
                     /pseudo
     CDS             complement(4408..4560)
                     /locus_tag="VEP73_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
BASE COUNT          602 a         1653 c         1820 g          665 t
ORIGIN      
        1 tccacgcgat cgcgctgcgc tgccagctcc ggatcgagcc gcatctgcgg cggtacgggg
       61 acgcggaggc ggggcggctg gtcgacctgt tcggcgagcc cgcccggtgg ggcgacaccc
      121 tgaagccgct gcagttcgcc aacctctcgg cgatggtgca ggggttcacg gggagcaccg
      181 aggtcgacct gcccgtgccg tgcacctacg acttcgaggt ggtctcggcg aagtacctcc
      241 actccctcga cgacggcgag gtaccgctcc tcctcctgtt cagcggcacc gtgttccgga
      301 ggggggagac cgggttctcg gtcgagcagg tgccgtggaa caaggaggcc acctaccgcc
      361 tgcccgtccg ggtgtggcgc gagacgatgg accgctactt cccgaacagc ggctggatcc
      421 gcatccggcg ggacacgctc gacgcgctct cgcgcttcaa gtcgcgcgag gcgctcccga
      481 cctgggacga gacgctggag acgctgctca agcaggccgg ggaggaggcg ccgtgagctt
      541 cgagcgcgcc cgcaaggtgg cggacgccat cctgttcgag gggtacgtcc tctacccgta
      601 ccgggcctcg gcgcagaaga accaggcgcg gtggcagttc ggggtgctcg tcccccgagc
      661 gtggagcgag ggcggctcgg gcgaggcgtg gagctcgcag accgagtgcc tggtcgagcc
      721 gggggacggg gccgcgctgg aggtccgggt ccggttcctg cagctgcagg cgaggacggt
      781 cgaggaggcc gtcgccggcg gcgggttccg gccggtcgcc tccctcgagg tcgacggcgc
      841 gccgctcgcg gcctgggacg agggggtcga gcgggaggcc ggcgcgacgc tgccggtcgg
      901 cgagctcctc gcgggcgagc ggacggtccc ggtggagctt ccgggcggcc gggagtccga
      961 gcccgtccgg cgggcgtctg gcgagctcgc gggccgggtg gtccgcgagc ggtggcccct
     1021 ctcgggacgc atctcggtcg ctgccgagcg cctcgacggg ccgtacggcg tcgtgcggct
     1081 ccgcgtgcgc gtcgagaacc tcaccccctg ggcggcgccg ggggccggcc gggacgaggc
     1141 ggtgcgccac tcgctcgccg gcgcccacag cctgctcgcg gtcgaccgcg gggggttcct
     1201 ctcgctcctc gacccgccgg agtgggccgg ccccgcggcc gcggcctgcg ccaacgtcgg
     1261 gacgttcccg gtgctcgtcg gggagccggg gcagcgggac gtggtgctct cgtcgccgat
     1321 catcctctac gaccacccgg ccgtggcccc ggagagccct ggcgacctgt tcgacgccac
     1381 cgagatcgac gagatcctca cgctgcggac gatggccctc accgacgagg agaagcggga
     1441 ggcgcgtggg accgacgacc gcgccgccgc gatcatcgac cgggtcgacg cgatgcccgc
     1501 cgaggtgctc gaccggctcc acggcgcggt ccggtacctg cgggacgcga ccgggccggg
     1561 aggcgcccac gaccggggcg cgcccgtgcc cggggcggac ggcccgccct cgtccacgcc
     1621 gtggtgggac cctggcgccg acgcctcggt ctcacccggg accgacagcg tgcgggtctc
     1681 cggggtcgag gtggccaggg gcagcctcgt ccgcctccgt cccgggcggc gccgggcgga
     1741 cgcgcaggac atgttcctcg aggggcgcgt cgcgaccgtc gaagcggtcc tgcacgacgt
     1801 cgacgagggc aggcacctgg cggtgacgct ggcggacgac ccggccgccg ccgagctgca
     1861 ccggtggcac gggcgcttcc tctacttctc cccggacgag gtcgagcccc tggacgccgg
     1921 ggtgtcccga tgagtggcgc caggaagatc ctggtcgcgg gggtgggcaa catcttcctc
     1981 ggcgacgacg gcttcggggt cgaggtggcg cggcgcctgg ccggcgagtc gccgcccggc
     2041 ggggtcacgg tggccgactt cggcatcagg ggcgtccacc tcgcctacga gttgctcgag
     2101 ggctacgaca cgacgatcct ggtggacgcc gccccccggg gcggcgagcc gggaaccgtg
     2161 tacgtggtcg aggtcgacgt cgacgacccg ggcgccggca tcccgtccga gcgggccggc
     2221 gaggagggcg cgctggtgga cgcccacggc atggagccgc aggcggtctt cgggctgctc
     2281 agggcgctcg gcgccgcggc agggcgggtg ctcgtggtcg gctgcgagcc ggccgaggtg
     2341 accgaccgga tcgggctcag cgaccctgtc gcccgggcgg tggacgaagc ggtccgcgtg
     2401 gtcggcgagc tcgtgcgtga cgagctcggg gaggcctcct ccggtgcctg gggcggggcc
     2461 ggggtcggga aggcccgccg ggggtccggg ggctcgagca gcgaagcggt agcccccggc
     2521 tcgttggagg gaggaggagc gtgatgggaa cccttcgccg cgtggtcgtc ggcgccgtcc
     2581 tggtcggcct ggcggccctg gtcgtccagt cgctgcccga cgtggtccga tacctgaaga
     2641 tcaagtcgat gtaaccatcc agtgaggagg gcgatgcacg aggtcgggtt gtgcgagtcg
     2701 atcgtcgacg cggtcgagcg ccgcgccgcc ggtcggcggg tgaggcgcgt gcgggtccgg
     2761 gtcggggtcc tccaccgggt cgtcgagccc gccctcgacc aggcgttctc gctcgtggcg
     2821 ggcgggacgg tggccgaagg ggccgcggtc gacctggtga cggtcgcggc ccgggtcgcc
     2881 tgcgccgcct gcgggcacga ggcggagtcc gacggcctgg tggtcgcctg cccccgctgc
     2941 ggcgcgaccg agctcgagct caccggcggg gacgagctcg tgctcgaatc catccagttc
     3001 gaggcgccgg cctgaccggt ggcggaagga gcacgagcgg atgtgcctcg ggattcccgg
     3061 agaggtcgtc gaggtcctgc atgaccgtcc cgacctggcc aaggtcgacg tcagcggggt
     3121 caggcgcgcc atcaacatcg gcctgctgga aggcgagacg ctcgagccgg gcgactggat
     3181 cctgatccac gtcgggttcg cgctgtccaa gatcgacgag gaggaggcca aggccgcgct
     3241 ggacttcctc gagggcatcg ggcaggcgta cgccgacgag ctggccgccc tgcaggcgtc
     3301 ccggatcgac tagcagcccc gaccgaagag gagatgacgc gccttgcgct tcgtcgacga
     3361 gttccgcgac gcggagaagg cccaggcgct cgccgcccag atcgcgtccc tgtgcgagcc
     3421 cgggcgccac tacaagttca tggaggtctg cgggggccac acccacacga tctacaagca
     3481 cggcctggag gactacctgc ccgagcagat cacgctggtg cacgggccgg gctgcccggt
     3541 gtgcgtgatc cccatgggcc gggtggacga cgccatcgac gtcgccgagc agcccgacgt
     3601 gatcatgacc tcgttcgggg acatgatgcg ggtcccggga ggccgcgggt cgttcttcga
     3661 ctcgaaggcc aagggcgccg acatccggat ggtctactcg ccgctggacg cgctcaaggt
     3721 ggccaggcag aaccctgaca ggcgggtcgt gttcatggcc atcgggttcg agacgaccgc
     3781 gccgtcgagc gcgatgaccg tcctgcgggc ggccgccgag gggctcgaca acttctcgat
     3841 cttctgcaac cacgtcacga tcatcccggc catcaaggcc atcctcgact cgcccgacct
     3901 gcgcctcgac gggttcatcg gcccagggca cgtctccacc gtgatcggct gccgcccgta
     3961 cgagttcatc gccagcgagc acggcaagcc gctggtctgc gccgggttcg agccgctcga
     4021 catcctgcag tccgtctaca tgctcctgcg gcagctcgcc gagggccgct ccgaggtcga
     4081 gaaccagtac acccgcgtcg tcccctggga cggcaaccgc aaggcgctcc aggtcatcgg
     4141 cgaggtcatg gagctgcggc cgtacttcga gtggcgcggg ctcgggttca tctcccactc
     4201 ggccctcagg gtgcgccagg agtacgcgcg gttcgacgcc gagcggatct tcgagatccc
     4261 cggcgtgcgc gtcgccgacc ccaaggcgtg ccagtgcggc gaggtcctga agggcgtgct
     4321 caagccctgg gagtgcaagg tcttcggcac cgcgtgcacc ccggagacgc cgatcgggac
     4381 ctgcatggtg tcctccgagg gcgcctgcgc cgcgtcctcg agcggctcga gcagcgggtt
     4441 gcggaaggcg tccaggaaga tcgcctcgac cagcgtcctc gtcgccttgc cgcccgcccc
     4501 gtgggcgagg gtgacgcgct cctccttgac cctcgccttg cggcggcggg cccggtcgat
     4561 ccgctcgagg acctgctgct cgcggtcgag gtgcggctcg gtggcgtgct cgcggacggc
     4621 ccgctcggcc cagtccgcgg tccgctcctc ctggttcacc ggagcacccc catgtgtgtc
     4681 ccgttcggca cgccgaggcg tgcctccgct tcggggttcc ccggacccct ccggggtcac
//
LOCUS       DASPYY010000352         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2589046, whole genome shotgun sequence.
ACCESSION   DASPYY010000352 DASPYY010000000
VERSION     DASPYY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589046"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..2087)
                     /gene="glgX"
                     /locus_tag="VEP73_05435"
     CDS             complement(30..2087)
                     /gene="glgX"
                     /locus_tag="VEP73_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYT25907.1"
                     /translation="MTEIWPGSAFPLGATPSASGTGFAVASETADGVTLCLFDGQGRQ
                     TRLELPEYDAGVWHGFVPGVGPGQRYGYRASGPYDPSRGLRCNPAKLLLDPYAKAITG
                     GVAWDDSFAGDSKQDSAPFAPRSIVVDPAFDWGDDRPPDVRFADSVIYEAHVKGTTAT
                     HPGVPPELRGTYAGLAHPAVLEHLVGLGVTAVELLPVHRSVTNGTLAARGLVNYWGYD
                     PVGYFAPHEAYSAAVRAGRPGGQVAEFQAMVRAMHAAGLEVLLDVVFNHTGEGNERGP
                     TLCHRGLDNPGYYRLVPGDPAHYFDTTGTGNSLNLAHSACLRMVLDSLRHWVTVMRVD
                     GFRFDLAATLARQEDGRFDRLSAFFDVVTQDPVVSQVKLIAEPWDVGQPDSYDVGRFP
                     PQWSEWNGRYRDTVRDFWRGQDGQLGELATRLAGSPDLYGWSRRRPSASINLITTHDG
                     FTLRDLVSYDGKHNQANGEDNRDGSDDNRSWNCGAEGPTGDQAVLALRARQSRALLVT
                     LLLSRGVPMLLGGDELGRTQGGNNNAYCQDNPTSWHDWSAVDAGLLDFTRRLIAFRRA
                     HPVLRRRRFATARDLGWYTPAGTPMTDADWQAPYARSVAVHLDGMASPDLDRSGRPQL
                     DDDLLILVNAWWEPVTFTLPEVGHAADWRLELDTYDPEATGTPAADPAPVGPRSVLVR
                     APH"
     gene            2217..3080
                     /locus_tag="VEP73_05440"
     CDS             2217..3080
                     /locus_tag="VEP73_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYT25908.1"
                     /translation="MSTAQTIVLGAIAGSTIFLGLPLGRVRMASLRGRAFGNAVSAGI
                     LVFLLVDILAHATGPVEQAMEQAKDGEAAWGHFAGLGLVYVLGFGAGLVGLLYATRLW
                     APRRRTGSGQGPRTSIGPGAMAAAEAELAQPAAQAAALRVGMSIATGIGLHNFSEGLA
                     IGQTAKSGAISLAALLIMGFALHNATEGFGIVGPLAAANVRASWGWLGLAGLIGGAPT
                     FLGAVVGTSFDSVFVFVGCLALAAGAILYVLGEVLPVGRRLSWEVTLWGLLAGFVLGV
                     ATDLILVAVGG"
     gene            3154..>3304
                     /locus_tag="VEP73_05445"
     CDS             3154..>3304
                     /locus_tag="VEP73_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT25909.1"
                     /translation="MTAERVAVIGGGLVGASAAYRLACHGARVALVDRADQGQATAAG
                     AGILAP"
BASE COUNT          392 a         1189 c         1253 g          470 t
ORIGIN      
        1 tcggcaacgt tgccgcgctc ccgaggggat cagtgtgggg cgcgaaccag caccgaccgc
       61 ggccccacgg gtgccgggtc ggcggcgggc gtcccggtcg cttcggggtc gtaggtgtcg
      121 agctccagcc gccagtccgc ggcgtggccg acctcgggga gggtgaaggt gaccggctcc
      181 caccaggcgt tgacgaggat gagcaggtcg tcgtcgagct gcggccgccc ggagcggtcc
      241 aggtccggcg acgccatccc gtccaggtgc accgcgacgc tcctggcgta cggcgcctgc
      301 cagtccgcgt cggtcatcgg cgtgccggcc ggggtgtacc agcccaggtc gcgtgctgtc
      361 gcgaagcggc gccggcgaag gacggggtgg gcgcggcgga aggcgatcag gcggcgggtg
      421 aagtcgagca ggccggcgtc caccgccgac cagtcgtgcc aggaggtcgg gttgtcctgg
      481 cagtaggcgt tgttgttgcc gccctgggtg cggccgagct cgtcgccgcc gagcagcatc
      541 ggcaccccgc gcgacagcag cagggtgacg agcagcgcgc gggactgcct ggcccgcagg
      601 gcgagcacgg cctggtcgcc ggtcgggcct tccgcgccgc agttccagga ccggttgtcg
      661 tcgctgccgt cccggttgtc ctcgccgttg gcctggttgt gcttgccgtc gtaggagacc
      721 aggtcgcgca gggtgaagcc gtcgtgggtg gtgatcaggt tgatggacgc cgacggccgc
      781 cgccgcgacc agccgtacag gtccggtgag ccggcgagcc gggtcgcgag ctcgccgagc
      841 tggccgtcct ggccgcgcca gaagtcccgc accgtgtcgc ggtacctgcc gttccactcg
      901 ctccactggg gtgggaagcg gccgacgtcg tagctgtcgg gctggcccac gtcccacggc
      961 tcggcgatca gcttgacctg ggacacgacg gggtcctggg tcaccacgtc gaagaaggcc
     1021 gacagccggt cgaagcggcc gtcctcctgc cgggcgaggg tggctgccag gtcgaaccgg
     1081 aacccgtcga cgcgcatcac ggtgacccag tggcgcagcg agtcgagcac catccgcagg
     1141 caggccgagt gcgcgaggtt caggctgttg cccgtgccgg tggtatcgaa gtagtgggcc
     1201 gggtcgcccg ggaccagccg gtagtacccc gggttgtcca gcccacggtg gcacagcgtc
     1261 gggccgcgct cgttgccctc gccggtgtgg ttgaagacca cgtcgaggag cacttcgagg
     1321 ccggccgcgt gcatcgcgcg caccatcgcc tggaactcgg cgacctgccc gccgggccgg
     1381 cccgcccgga cggcggcgga gtacgcctca tgcggcgcga agtagccgac cgggtcgtac
     1441 ccccagtagt tgaccaagcc tcgcgcggcg agcgtgccgt tcgtgaccga ccggtgcacc
     1501 ggcagcagct cgacggcggt gacccccagg ccgaccaggt gctcgagcac cgccggatgg
     1561 gccaggcccg cgtaggtgcc gcgcagctcc ggcgggacgc ccggatgggt cgcggtggtc
     1621 cccttgacgt gcgcctcgta gatcaccgag tccgcgaagc ggacatccgg cggccggtcg
     1681 tcgccccagt cgaacgccgg gtcgacgacg atcgagcgtg gcgcgaacgg ggccgagtcc
     1741 tgcttgctat cgccggcgaa gctgtcgtcc caggcgacgc ccccggtgat cgccttggcg
     1801 tacgggtcga gcagcagctt ggccgggttg cagcgcagcc cccgcgacgg gtcgtacggg
     1861 ccgctcgccc ggtagccgta ccgctggccc ggcccgacac ccggcacgaa cccgtgccac
     1921 acccccgcgt cgtactccgg cagctcgagc cgcgtctgcc gtccctgccc gtcgaacagg
     1981 cagagcgtga ccccgtcggc ggtctcggac gcgacggcga accccgtgcc gctcgccgac
     2041 ggggtcgccc cgagcgggaa cgccgatccc ggccagatct cggtcatggc cgccagggtg
     2101 cacgcgcccc cggcccggcg gcaaggacgg cttgcatgtc ggcggccaca gcgctagcgt
     2161 cccctcggac ttaggcagcc ctaaacagcc ctggacttga cttccggagg agccgcatgt
     2221 cgaccgccca aacgatcgtg ctcggcgcca tcgccgggtc cacgatcttc ctcggcctcc
     2281 cgctcggccg tgttcgcatg gccagcctgc gcggccgggc gttcggcaac gccgtctccg
     2341 ccggcatcct ggtgttcctg ctggtcgaca tcctcgccca cgccaccggg ccggtcgagc
     2401 aggccatgga gcaggccaag gacggcgagg cggcctgggg ccacttcgcc gggctcggcc
     2461 tggtgtacgt gctcggcttc ggggccggcc tggtcgggct gctgtacgcc acgcgcctgt
     2521 gggcgccccg ccggcgcacc ggcagcggcc agggtccgcg caccagcatc ggcccggggg
     2581 cgatggccgc cgccgaggcc gagctggccc agccggccgc gcaggcggcg gcgctgcggg
     2641 tcggcatgtc gatcgcgacc ggcatcggcc tgcacaactt ctccgagggg ctcgcgatcg
     2701 gccagacggc caagagcggc gccatctcgc tggcggcgct gctcatcatg ggcttcgccc
     2761 tccacaacgc caccgagggg ttcggcatcg tcgggccgct cgccgcggcc aacgtgcggg
     2821 cgtcctgggg ctggctcggg ctggccgggc tgatcggcgg ggcccccacc ttcctcggcg
     2881 ccgtggtggg cacctcgttc gactcggtgt tcgtgttcgt gggctgtctc gcgctggccg
     2941 ccggcgccat cttgtacgtg ctgggtgagg tgctgcccgt cggccgccgc ctgtcctggg
     3001 aggttaccct gtggggcctg ctggccggct tcgtcctcgg cgtggccacc gacctcatcc
     3061 tcgtcgccgt cggcgggtag cgcaggatcc gccggggagg gcgccgttca tccggcgggt
     3121 cgccgagctg ctggcctggg agctcctcgg cgcgtgaccg cggagagggt ggcggtgatc
     3181 ggcggcgggc tcgtcggcgc gagcgccgcc taccggctgg cgtgccacgg cgcgcgggtg
     3241 gccctggttg accgcgccga ccagggacag gcgaccgcgg ctggcgccgg gatcctcgcg
     3301 ccgg
//
LOCUS       DASPYY010000353         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2027863, whole genome shotgun sequence.
ACCESSION   DASPYY010000353 DASPYY010000000
VERSION     DASPYY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027863"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>1684
                     /locus_tag="VEP73_05450"
     CDS             164..>1684
                     /locus_tag="VEP73_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT25910.1"
                     /translation="MPQALLVDDDEPFLAALAEAVSRESFSTRTATSLAAAKLAIAGE
                     APDVVLIDLHLPDGNGMELLQELSAFPGTEVVLITGNASVETAVEALRRGASDYLVKP
                     LDLGRIKTVLANVSRTRELKLLAASLPWARRATSDSGMPVVFGRYEVEAEIGEGAMGR
                     VFRCRDPLVGRPVAVKTVKSEYLSGDTRAEYLRRFRREAQAAGILSHPGIVGIYDVGD
                     DYMVMEYVEGATLQQLIRDERALDFSSALRVLAPLAEALDYAHQAGVIHRDIKPSNIM
                     VQPDGRPKLMDFGVARLESSTATRSGRVLGSPSYMAPEHILGEVPTPGADLFSFAVVA
                     YEALTGRRPFTGDSVPAIVYRVVHEPAPAPRSLNPALPAACDAVFARALAKKRDERYP
                     DATKFVAALLGGDAGAALPAISPDPLLKSLVSSAAPPASAPVAAGAHEATPAPAAVKS
                     SGELPTTTLNTRMSRLRFAARRRWRQGLAAGLALAAAVAVSSWANRRPAPEADPSGP"
BASE COUNT          220 a          631 c          604 g          229 t
ORIGIN      
        1 gatcgagacg agcctgaagc ctgctcctgc tccgcgcccg tgcggcgcgg gggggggatt
       61 tgtggtgtag ctaacacccc gccggacgac gctgtcaata gctgtatgat cttcccgacg
      121 aggccgccac cccctccacc cggcggccgg cgccggaggt cggatgccgc aagcgctgct
      181 cgtggacgac gacgagccgt tcctcgccgc cttggccgag gccgtttccc gcgagagctt
      241 ctcgacccgc accgccacca gcctggccgc cgccaagctg gccatcgccg gcgaggcccc
      301 cgacgtggtc ctcatcgacc tgcacctgcc cgacggcaac ggcatggagc tgctccaaga
      361 actgagcgcg ttccccggca cggaggtcgt gctcatcacc ggcaacgcca gcgtggagac
      421 cgcggtggaa gcgctgcggc gcggcgcctc cgactacctc gtcaagcccc tggacctcgg
      481 ccgcatcaag accgtgctcg ccaacgtctc gcgcacccgc gagctgaagc tgctcgcggc
      541 cagcctgccc tgggcccggc gcgcgacgag cgacagcggg atgccggtcg tgttcggacg
      601 ctacgaggtg gaggccgaga tcggcgaggg ggccatgggc cgcgtcttcc gctgccgcga
      661 cccgctcgtc ggccgcccgg tggcggtgaa gaccgtgaag tcggaatacc tgagcggcga
      721 cacgcgcgcg gaatacctgc gccgcttccg gcgggaggcg caggccgccg gcatcctgtc
      781 ccaccccggc atcgtgggca tctacgacgt gggcgacgac tacatggtga tggagtacgt
      841 ggaaggcgcc acgctccagc aactgatccg tgacgagcgc gcgctcgact tttcgtccgc
      901 gctgcgggtg ctggctccgc tggcggaggc gctcgactac gcgcaccagg cgggcgtgat
      961 ccaccgggac atcaagccct ccaacatcat ggtccagccc gacggccggc cgaagctcat
     1021 ggacttcggc gtggcccggc tggagagctc caccgccacg cgctcggggc gcgtgctcgg
     1081 ctcgccgtcc tacatggcgc ccgagcacat actgggggag gtgccgacgc cgggggccga
     1141 cctgttctcc ttcgcggtcg tggcctacga ggcgctgacc gggcggcggc cgttcacggg
     1201 cgactcggtg cccgccatcg tctaccgcgt cgtgcacgag ccggcaccgg ccccgcgcag
     1261 cctcaacccg gccctgcccg ccgcctgcga cgcggtgttt gcgcgggcgc tggccaagaa
     1321 gcgcgacgag cgctatccgg acgcgacgaa gttcgtggcg gcgctcctgg gaggcgacgc
     1381 cggagcggcc ctgcccgcca tctcgccgga tccgctgctc aagtcgctgg tctcctcggc
     1441 ggcgccgccg gcctcggctc cggtggcggc cggggcccac gaggccacgc cggcgccggc
     1501 ggcggtgaag tcgtcagggg agttgccgac gaccacgctc aatacgcgca tgtcgcggct
     1561 gcggttcgcg gcccgccggc ggtggcgcca gggcctggcc gccggcctcg cgctggccgc
     1621 ggcggtggcc gtctcgtcgt gggcgaaccg gcggccggct ccggaggccg acccctccgg
     1681 cccg
//
LOCUS       DASPYY010000354         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_762650, whole genome shotgun sequence.
ACCESSION   DASPYY010000354 DASPYY010000000
VERSION     DASPYY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762650"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VEP73_05455"
     CDS             <1..819
                     /locus_tag="VEP73_05455"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYT25911.1"
                     /translation="VHDLTMVTHPEWHEPSKVWYFRWAMRRAAAVADRVLCVSAATAR
                     DATRHLGVPPDRIDVTPLGTELRPASGEAVRDVRARLGLDGPYVLGLGTLEPRKDLPT
                     LVRAFAALAGELPHTLVLAGLAGWGAGALAEAVRSSGVAGRVRLPGYVPEADKAALFT
                     GADLFVYPSRYEGFGLPVLEAMACGVPVVTTTGGSLPEVAGDGALLVDPGDPGTLAAA
                     MARLLGDRAARDRLARRGRDRAAAQTWDRCAALTAEAYRRAAAASGREVRSRAP"
     gene            874..>1681
                     /locus_tag="VEP73_05460"
     CDS             874..>1681
                     /locus_tag="VEP73_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYT25912.1"
                     /translation="MQAVILAGGFGTRLRPLTSTRPKPLIPFANEPFLLHNLRQLGTA
                     GFTEVVLSTMYLPEAFDELIPMARKAGVEVVLSSEDTPMGTSGAVKRLAHLLDETFLV
                     LNGDILVDLDYARLVALHAERRAAATLALVRVPDPSAFGLVPVDPDGRVQAFLEKPGP
                     DEDRWVTDLINAGVYVLEPAVLEHVPAGQPSSFERNLFPNLLASGAPVHGYELHGYWK
                     DLGTPIAYLQAQADLLEGRLHLPINATERRRNQYVADGAAIADGAVVRGPV"
BASE COUNT          195 a          669 c          602 g          215 t
ORIGIN      
        1 gtccacgacc tcaccatggt gacccacccg gagtggcacg agccgagcaa ggtctggtac
       61 ttccgctggg ccatgcgccg ggcggccgcg gtcgcggacc gggtcctgtg cgtctccgcg
      121 gccaccgccc gcgacgcgac ccgccacctc ggcgtcccgc ccgaccggat cgacgtcacc
      181 ccgctcggca ccgagctgcg gccggcctcc ggggaggcgg tcagggacgt gcgtgcccgc
      241 ctcggcctgg acggcccgta cgtgctgggg ctgggcacgc tcgagccccg caaggacctg
      301 cccacgctgg tccgcgcctt cgccgccctg gccggcgagc tgccccacac gctggtgctg
      361 gccgggctgg ccggctgggg cgccggggcg ctcgccgagg cggtgcggtc cagcggggtc
      421 gccggccggg tccggctgcc cggctacgtc cccgaggcgg acaaggcggc gctcttcacc
      481 ggcgccgacc tgttcgtgta cccgagccgc tacgaggggt tcgggctgcc cgtgctcgag
      541 gcgatggcgt gcggcgtgcc cgtggtcacc accacgggcg ggtcgctgcc cgaggtggcc
      601 ggggacgggg cgctgctcgt cgaccccggg gatccaggca cgctcgccgc cgccatggcc
      661 cggctgctcg gcgaccgggc ggcccgggac cgcctggcac ggcgcggccg ggaccgggcc
      721 gcggcccaga cgtgggaccg ctgcgcggcg ctcaccgccg aggcctaccg ccgcgccgcc
      781 gccgccagcg ggcgggaagt ccgcagccgt gcaccgtaga ctttcgtcgt cggccgctgg
      841 gccggccacc gactcgtccc aggagcgcca tccgtgcaag ccgtcatcct cgccgggggc
      901 ttcggcaccc gcctgcgccc gctgaccagc acccggccga agccgctgat cccgttcgcc
      961 aacgagccgt tcctgctgca caacctgcgc cagctcggca ccgccgggtt caccgaggtg
     1021 gtcctctcga ccatgtacct gcccgaggcc ttcgacgagc tgatcccgat ggcacgcaag
     1081 gccggcgtcg aggtggtcct ctcctccgag gacaccccga tgggcacctc gggcgcggtc
     1141 aagcgcctcg cccacctgct ggacgagacc ttcctcgtcc tgaacggcga catcctcgtc
     1201 gacctggact acgcgcgcct ggtcgcgctg cacgccgagc ggcgcgcggc ggccaccctg
     1261 gcgctggtgc gcgtgcccga cccgagcgcg ttcgggctcg tcccggtcga cccggacggg
     1321 cgcgtgcagg cgttcctcga gaagcccggc ccggacgagg accgctgggt gaccgacctc
     1381 atcaacgccg gcgtgtacgt gctcgagccg gccgtgctcg agcacgtacc cgccgggcag
     1441 ccgtcgtcgt tcgagcggaa cctgttcccg aacctgctcg cttcgggcgc gccggtccac
     1501 gggtatgagc tccacggcta ctggaaggac ctgggcaccc ccatcgccta cctccaggcc
     1561 caggccgacc tcctcgaggg ccggctgcac ctgccgatca acgcgaccga gcgccggcgc
     1621 aaccagtacg tcgccgacgg ggccgccatc gcggacggcg ccgtggtccg cgggccggtc
     1681 a
//
LOCUS       DASPYY010000355         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2027937, whole genome shotgun sequence.
ACCESSION   DASPYY010000355 DASPYY010000000
VERSION     DASPYY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027937"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1884)
                     /locus_tag="VEP73_05465"
     CDS             complement(<1..>1884)
                     /locus_tag="VEP73_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYT25913.1"
                     /translation="LLRRGIVVTFDRLVDEVFGKEPPEEARNALQTYVARLRHALGPA
                     AAVVVTRAPGYVLDVPGHAVDTERFTTLLGKARHMHSPTAALALLDEALALWRGPAYA
                     EFAETFARGETLRLHELWLAAQEDRAALLLRLGRLAESTAALEAIVAHEPWRERAVEL
                     LVSALAQAGRAPHALAVYTRYRDRLRDELGLDPSLRLRRLEEQVLRGELPPVQPHHAK
                     RQPRTLPARATSFVGREQELALVHKTLADGRLATLVGPGGVGKTRLAQQAAAAQGSVW
                     WVDLAPLRDQDAVPHALADALDLDVQPGTPVLDTLRAWAGSARGLLVVDNCEHLLAAV
                     AGVAEELLGACSALRLLATSRERLGVEGEQVLVVPPLAVPAPDAEEVRTPAVRLFCDR
                     ARAADPGFAPGREALMRVGEVCRTLDGLPLAIELAAARIGTLTVDDLADRLDARFELL
                     RTTNRGGGDARHHTLRRVVDWSFDLLTPEEQRLFLRLSVFAAAFDLAAAEKVVADNDL
                     PAGRVADLTARLADRSMLTRPGHSGVGRYRMLETLRAFAAGRLPTAEAERLRDRHAGF
                     MVDLAERAEAGLYGPDEPAWARRVEAWLDDLRAAWSWARDAGEVDVAVRLAAALTRFA
                     YWRV"
BASE COUNT          260 a          671 c          733 g          220 t
ORIGIN      
        1 cacgcgccag tacgcaaagc gcgtgagggc cgcggcgagc cgcaccgcga cgtcgacctc
       61 gccggcgtcc cgcgcccagc tccacgccgc ccgcaggtcg tccagccacg cctcgacccg
      121 gcgggcccag gccggctcgt ccgggccgta gagccccgct tcggcgcgct cggcgaggtc
      181 gaccatgaac ccggcgtggc ggtctcggag gcgctcggcc tcggcggtgg gaagccggcc
      241 ggcggcaaag gctcgcaggg tctccagcat ccggtagcgg ccaacgccgc tgtggcccgg
      301 ccgggtcagc atggagcggt cggccagccg ggccgtcagg tcggcgacgc ggccggcggg
      361 caggtcgttg tcggccacga ccttctcggc ggcggcgagg tcgaacgccg cggcgaacac
      421 cgacagccgc aggaacagcc gctgctcctc tggcgtgagc aggtcgaagg accagtcgac
      481 gactcgacgg agggtgtggt ggcgcgcgtc accgccgccg cggttggtcg tgcggagcag
      541 ctcgaagcgc gcatcgagcc ggtcggcgag gtcgtcgacg gtcagcgtgc cgatccgggc
      601 ggcggccagc tcgatggcga gcgggaggcc gtcaagggtg cggcagacct cgccaacccg
      661 catgagggct tcccggccgg gcgcgaagcc agggtcggcc gcgcgggccc ggtcacagaa
      721 cagccggacc gcaggagtgc ggacctcctc ggcgtccggc gcgggcacgg caagtggtgg
      781 gacgaccagg acctgctcgc cctcgacgcc gagccgctcg cggctggtcg ccaacagccg
      841 aagcgcggag caggccccca atagctcctc ggcgacccct gccacggcgg cgagcaggtg
      901 ctcgcagttg tcgacgacca ggagcccacg agcgctacct gcccacgctc ggagggtgtc
      961 cagcactggg gtgcccggct ggacgtcgag gtcgagcgcg tcggcgagcg catgcggtac
     1021 ggcgtcctgg tcgcgaagcg gggccaggtc cacccaccag accgaccctt gggcggccgc
     1081 ggcctgctga gccagccggg tcttgccgac cccgcccggg ccgaccagcg tggccagccg
     1141 cccgtcggcg agggtcttgt ggaccagggc gagctcttgt tcccggccaa cgaacgaggt
     1201 cgctcgggcg gggagcgtgc gaggctgccg tttggcgtgg tggggctgga cgggcggcag
     1261 ctccccgcgg agcacctgct cctcgagacg ccgcagcctg agcgaggggt caaggccgag
     1321 ctcatcgcga agccgatcgc gatagcgggt gtagacggcg agcgcgtggg gggcccggcc
     1381 ggcctgggca agagccgaga ccaacagctc gacggcccgc tcccgccagg gctcatgcgc
     1441 cacgatggct tccagagcgg cggtgctctc ggcgaggcgg cccagccgaa gcagcagggc
     1501 tgcccggtct tcctgggcgg cgagccagag ctcgtggagg cgcagcgtct cgccccgcgc
     1561 gaaggtctca gcgaactcgg cgtatgccgg gccacgccac agcgcgagtg cctcgtccag
     1621 cagcgcgagc gcggccgttg gcgagtgcat gtggcgcgcc ttgccgagca gcgtggtgaa
     1681 ccgctccgtg tcgaccgcgt gccccggcac gtccaggacg tagccgggtg cgcgcgtgac
     1741 gaccaccgcc gcggccggtc cgagggcgtg gcgcagccgc gcgacgtagg tctgcagcgc
     1801 gttgcgggcc tcctcgggcg gctctttgcc gaacacctcg tcgacgagtc ggtcgaacgt
     1861 gacgacgatg ccgcggcgga gcag
//
LOCUS       DASPYY010000356         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1325907, whole genome shotgun sequence.
ACCESSION   DASPYY010000356 DASPYY010000000
VERSION     DASPYY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325907"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /gene="fbiC"
                     /locus_tag="VEP73_05470"
     CDS             <1..456
                     /gene="fbiC"
                     /locus_tag="VEP73_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase"
                     /protein_id="HYT25914.1"
                     /translation="EGYRNWARHLGVLRDIQKDTGGFTEFVPLPFVHMAAPIYRKRLS
                     RPGPTFREALLVHAVARIAYAGRIPNIQVSWVKMGADGVRQALLAGVNDLGGTLMDEN
                     ISRAAGASHGQLMEAEDFARIVEPLGRRLEQRTTVYGRVATEPGPAAVA"
     gene            complement(465..1523)
                     /locus_tag="VEP73_05475"
     CDS             complement(465..1523)
                     /locus_tag="VEP73_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYT25915.1"
                     /translation="MSGLLGRLPLSGWQRVAAAVVLAVLLLAAGTAAAAARSGGPSGR
                     ILSGVTIDGVDVGGMTRAQAVAAVGADAARSLHRGIIVKVGDKRFRMTPAGVGRGKAV
                     DQAVDRALAGPRLSFAGRVWHEVAGRPVTMAVRLETTTRDERVAAFARRIAPRVAVAP
                     QDAALKLEDGRLVARHARSGRYLDVAAATRGLLKVLAEAGRTDARLALAPVAPKLLDE
                     QLGLTIVIDKSVNRLTLYRGLSVVKTYGVATAKTGFVTPSGTWTVIAKLVNPTWHNPA
                     PDTWGAGEPLVIPPGPGNPLGTRALALNAAGIFIHGTPAAWSIGSHASHGCIRMRIPD
                     AEDLYPRVPTGTRVLIHS"
BASE COUNT          200 a          589 c          577 g          200 t
ORIGIN      
        1 gagggctacc ggaactgggc ccgccacctc ggagtgctgc gggacatcca gaaagacacc
       61 ggcggcttca ccgagttcgt gccgctgccg ttcgtccaca tggccgcgcc catctaccgc
      121 aagcggctgt cgcggcccgg cccgacgttc cgggaggcgc tgctcgtgca cgccgtcgcc
      181 cggatcgcct acgccggccg catcccgaac atccaggtct cctgggtgaa gatgggggcg
      241 gacggggtgc gccaggcgct cctggccggc gtcaacgacc tcggcggcac cctgatggac
      301 gagaacatca gccgggccgc cggtgcgagc cacggccagc tcatggaggc cgaggacttc
      361 gcccgcatcg tcgagccgct cggccggcgg ctcgagcagc gcaccaccgt ctacggccgg
      421 gtcgccaccg agcccggccc ggccgccgtc gcctgagcgc tggctcagga gtggatgagc
      481 acccgcgtcc cggtcggcac gcgcgggtag aggtcctcgg cgtccgggat gcgcatcctg
      541 atgcagccgt gcgaggcgtg gctgccgatc gaccacgccg ccggcgtgcc gtggatgaag
      601 atgcccgcgg cgttcagggc gagggcgcgg gtgccgagcg ggttgccagg gcccggcggg
      661 atcacgagcg gctcgccggc accccaggtg tccggggccg ggttgtgcca ggtggggttg
      721 accagcttgg caatgaccgt ccaggtgccg ctcggcgtga cgaaaccggt cttggcggtc
      781 gccaccccgt acgtcttcac gaccgagagc ccgcggtaca gggtcagccg gttgacgctc
      841 ttgtcgatca cgatcgtgag gccgagctgc tcgtcgagga gcttcggcgc caccggcgcg
      901 agcgccagcc gggcgtccgt gcggcccgcc tccgcgagca ccttgagcag gcccctggtg
      961 gcggccgcga cgtccaggta ccggccgctc ctggcgtgcc tcgcgacgag cctgccgtcc
     1021 tcgagcttca aggccgcgtc ctgcggggcg acggcgaccc gcggggcgat ccggcgcgcg
     1081 aacgcggcga cgcgctcgtc ccgggtggtc gtctcgaggc ggacggccat ggtcaccggc
     1141 cgcccggcga cctcgtgcca gacccttccc gcgaaggaga gccgcgggcc tgccagcgcc
     1201 cggtcgaccg cctggtcgac cgccttgccc cggccgacgc cggccggtgt catgcggaag
     1261 cgcttgtcgc cgaccttgac gatgatcccg cggtgcagcg accgggcggc gtcggcgccg
     1321 acggccgcga cggcctgggc ccgggtcatc cccccgacgt ccaccccgtc gatcgtcacc
     1381 ccggagagga tcctgccgga cggccctccg gagcgggcgg ccgccgccgc cgtgccggcg
     1441 gcgagcagca ggacggcaag gacgaccgcc gcggcgaccc gctgccagcc cgacagcggc
     1501 aatctcccga gaagcccgct catgcgtctc ctgcctcgaa ggtcgtgccg gccccgaccg
     1561 tcgtgg
//
LOCUS       DASPYY010000357         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_341950, whole genome shotgun sequence.
ACCESSION   DASPYY010000357 DASPYY010000000
VERSION     DASPYY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341950"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VEP73_05480"
     CDS             <1..979
                     /locus_tag="VEP73_05480"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HYT25916.1"
                     /translation="GHTGGTLDKLESIPGFRVDLGAEELLDQVERVGLAVAAQSADLV
                     PADKAMYALRDVTATVASTALIASSVMSKKLAGGAGCILLDVKAGSGAFMKDVPAATD
                     LARACVALGEAGGRRTAALVTDMDQPLGDLVGNAVEVREAVEALRGERPGRFTDLCLT
                     LTGHLAALAGVAPGAEEGRGAAARALERGAGLEKLRELVEAQGGDPRVVDDLSLLPRA
                     PVRLEVTAERAGWLAGVDAEAVGQVAAGLGAGRQRKDDPVDPAVGVELAVKVGDEVGA
                     GGRIGRVLARDEAAAGTAARELAAALRWSDRPCPAPDLVHQVILPEASR"
     gene            1103..1762
                     /locus_tag="VEP73_05485"
     CDS             1103..1762
                     /locus_tag="VEP73_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYT25917.1"
                     /translation="MQSPLYLLRDDPQLFVAFVIAIVVAITVHEFSHAAVATLEGDDT
                     ARSAGRLTLNPLSHLDPLGSIFLVLVGFGWGRPTPFNPARLHNQRVGAALVGLAGPAS
                     NFLLALLSALALRLSHPQGIDVFEANSSFTVKLLLRFVQLNVLLGVFNLLPIPPLDGS
                     RLLSILLPPSRQGIVYFLDQYGIFLLIGLLFLAPGLLAPVFTQLEDLVLRLVGVRFVG
                     L"
     gene            1904..>2379
                     /locus_tag="VEP73_05490"
     CDS             1904..>2379
                     /locus_tag="VEP73_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYT25918.1"
                     /translation="MDYVVKTEVFEGPFDLLLHLIARQRVDLWQVSVSSITEEYLAEI
                     KRMHELNLEVATEFLVVAATLLELKAARLLPGPDAGGDEVEALLSERDLLFARVLQYR
                     AYRHVAALFAARLAQQAAYVPRRAGADDLFRRIVPDLLVGVRPGELARLAAAALAPA"
BASE COUNT          264 a          918 c          869 g          328 t
ORIGIN      
        1 cggccacacc ggcggcacgc tcgacaagct cgagtccatc cccgggttcc gggtcgacct
       61 gggcgcggag gagctgctcg accaggtcga gcgggtcggc ctggcggtcg ccgcccagag
      121 cgccgacctc gtccccgccg acaaggcgat gtacgcgctg cgcgacgtga ccgcgacggt
      181 ggcgagcacc gcgctgatcg cctcgagcgt gatgagcaag aagctggccg gcggggcggg
      241 ctgcatcctc ctcgacgtca aggcggggtc gggcgcgttc atgaaggacg tgccggccgc
      301 caccgacctc gcccgtgcct gcgtcgccct gggcgaggcg ggcgggcgcc gcacggccgc
      361 gctcgtgacc gacatggacc agccccttgg cgacctcgtc ggcaacgccg tggaggtgcg
      421 ggaggccgtg gaggcgctgc ggggcgagcg tccgggccgc ttcacggacc tctgcctcac
      481 cctcaccggc catctcgcgg cgctcgccgg cgtcgcgccc ggcgccgagg agggccgcgg
      541 ggcggcggcc cgggcgctcg agcggggcgc cgggctggag aagctccgcg agctcgtcga
      601 ggcgcagggc ggcgacccgc gcgtcgtcga cgacctgtcc ctgctgccgc gggcccccgt
      661 ccgcctggag gtgaccgccg agcgggccgg ctggctggcc ggggtcgacg ccgaggcggt
      721 cggccaggtg gcggccggcc tcggggcggg gcggcagcgc aaggacgacc cggtcgaccc
      781 ggccgtgggc gtcgagctcg cggtcaaggt cggggacgag gtgggtgccg gcggccgcat
      841 cggccgggtg ctcgcccggg acgaggcggc cgccggcacc gccgcacggg agctggccgc
      901 cgccctgcgc tggagcgacc gtccctgccc ggctccggac ctggtccacc aggtgatcct
      961 gcccgaggcc tcgaggtgat cccgccccgg ggctgacggc ggccggccag gccgccgcct
     1021 catgctaagt tggcagggac cgcccgacgg ctcgcagcgg catcgggcct ggacctgtgc
     1081 gccgccggga gggagctcgt cgttgcagtc gcccctgtac ctgctgcggg acgacccgca
     1141 gctgttcgtg gccttcgtga tcgcgatcgt cgtggcgatc accgtccacg agttcagcca
     1201 tgccgccgtc gccacgctgg agggcgacga cacggcgagg tccgcgggcc ggctcaccct
     1261 caacccgctg tcgcacctcg acccgctcgg cagcatcttc ctggtcctgg tcggcttcgg
     1321 gtggggacgc ccgacgccgt tcaacccggc gcggctccac aaccagcgcg tgggggcggc
     1381 cctcgtcggc ctggccgggc cggccagcaa cttcctgctc gcgctcctga gcgccctcgc
     1441 gctccgcctc tcccatcccc agggcatcga cgtcttcgag gccaactcca gcttcaccgt
     1501 caagctgctc ctgcggttcg tgcagctgaa cgtcctcctc ggggtcttca acctgctgcc
     1561 catcccgccg ctggacggct cgaggctcct gtcgatcctg ctcccgccgt cgcggcaggg
     1621 gatcgtctac ttcctcgacc agtacggcat cttcctgctc atcggcctgc tcttcctggc
     1681 ccctgggctg ctggcgccgg tcttcacgca gctcgaggac ctggtcctcc ggctcgtggg
     1741 cgtccggttc gtgggcctct gaccgcgcgg ctcgacgcgc gccgtccggg cgccgccggc
     1801 ggcggagcgg taggctctcg gagacaccgg gccacgccgc cccccgcagc cggccggcgc
     1861 ggccggccgc cccggcccaa gcgacgaacc gacagcgaga cgcatggact acgtggtcaa
     1921 gacggaggtc ttcgaggggc cgttcgacct gctcctgcac ttgatcgcgc gccagcgcgt
     1981 cgacctctgg caggtctcgg tctcgagcat caccgaggag tacctggccg agatcaagcg
     2041 catgcacgag ctgaacctcg aggtggccac cgagttcctg gtcgtcgcgg ccaccctgct
     2101 cgagctcaag gccgcccgcc tgctgccggg ccccgacgcc ggcggcgacg aggtcgaggc
     2161 cctgctgtcc gagcgcgacc tgctcttcgc ccgggtgctg cagtaccggg cctaccggca
     2221 cgtcgccgcc ctgttcgccg cccggctggc ccagcaggcc gcctacgtgc cgcgccgggc
     2281 aggcgccgac gacctgttcc gccgcatcgt gcccgacctg ctcgtcgggg tccgtcccgg
     2341 ggagctcgcc cggctggcgg cggccgcgct cgcgcccgc
//
LOCUS       DASPYY010000358         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1044888, whole genome shotgun sequence.
ACCESSION   DASPYY010000358 DASPYY010000000
VERSION     DASPYY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044888"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VEP73_05495"
     CDS             complement(<1..529)
                     /locus_tag="VEP73_05495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25919.1"
                     /translation="MDVDIRPIAAEELPELARVFGTVFGDAWPDGAVAGLGRIFEPDR
                     SLCAFDPRDGMVATGGAYSLELTLPGGGGVPAAGLTLISVLPTHRRRGILRALIQRHF
                     EDVQARGEAVSVLTATESLIYGRFGYGMATSAAQLEIDRRHAAFAESVDTPGRVRMVD
                     AGEAARLLPEVFDRVR"
     gene            complement(618..1895)
                     /locus_tag="VEP73_05500"
     CDS             complement(618..1895)
                     /locus_tag="VEP73_05500"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HYT25920.1"
                     /translation="MAELSTETVFTWGAPPLKYGTGAIDELGHDLAQLGVERVLVVTD
                     RGVAETGIPERARHAAEEAGLKAEIYDGVHVEPTDESLREAAEYARAGEWDGFLAIGG
                     GSSIDTAKAMNLMTSSPGDVMDYVNKPVGEGRAPSGPLKPLVAVPTTAGTGSESTPVC
                     VLDILGLKVKSGISHPRLRPTLAVIDPLVTLTLPPTATAASGMDIVGHALESYTARRY
                     TSYPRKDAADRVAYCGSNPISDMWSERALGMINRSFRRAVLVGHDLEARSQMALAATF
                     AGMGFGNAGVHIPHACAYPIAGMVRDYRPEGYIGDEPMVPHGESVAVTAPASFRYTFP
                     ADPDRHVRAATLLGADPAGGRDPRELLPDALVGLMRDIGTPNGIGALGYGEDDVDELV
                     AGTMKQRRLLVIAPRDATEEDLAGIFRSSMANW"
     gene            complement(1982..2236)
                     /locus_tag="VEP73_05505"
     CDS             complement(1982..2236)
                     /locus_tag="VEP73_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25921.1"
                     /translation="MGKLEETRDQAEHWAEQARKTLSHVDNRGVTQLQESLYDRPYAH
                     LAGVVPEITYAHACAALSSAYSALHQNSSSGPVPGSLPSG"
     gene            complement(2170..2592)
                     /locus_tag="VEP73_05510"
     CDS             complement(2170..2592)
                     /locus_tag="VEP73_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HYT25922.1"
                     /translation="MRTSDATSAPRRVQLRRTNGWRKPPDAVVISRPSKWGNPYRVSE
                     HGLLGALALYRRHLTEHPELAEAARRELAGKTLACWCRPDQPCHGDILFEVLEQDQAH
                     GQDGQANDTPAQRGGTAWASSRRPATRRSTGQSKPAKP"
     gene            complement(2634..3032)
                     /locus_tag="VEP73_05515"
     CDS             complement(2634..3032)
                     /locus_tag="VEP73_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25923.1"
                     /translation="MSALVALTPLSAKAQSPEVTITVGATGTVQSRIVVTVPVQITCG
                     PLEVGTAQQSATIEQAAGHEIAQGSGFADQPIVCDGTAQPNTFTFLADPSGPPFHGGN
                     AAVSIGLFICSPSFVCISGGSGIQIVKLKG"
BASE COUNT          406 a         1072 c         1138 g          464 t
ORIGIN      
        1 ggcggacgcg gtcgaagacc tcgggcagca gccgggccgc ctcgccggcg tccaccatgc
       61 ggacccgccc cggggtgtcg accgactcgg cgaacgcggc gtgccgccgg tcgatctcga
      121 gctgcgccgc cgaggtggcc atgccgtacc cgaagcggcc gtagatgagc gactccgtcg
      181 cggtcagcac cgacaccgcc tcgccgcggg cctggacgtc ctcgaagtgc cgctggatca
      241 gcgcccgcag gatgccccgg cgccggtggg tgggcagcac cgagatcagc gtgagcccgg
      301 cggcggggac gcccccgccg ccgggcaggg tcagctcgag tgagtaggcg ccgccggtcg
      361 cgaccatccc gtcccgcggg tcgaaggcgc agaggctccg gtccggctcg aagatcctgc
      421 cgagcccggc gaccgcgccg tcgggccagg cgtcgccgaa gaccgtgccg aagacgcggg
      481 cgagctcggg gagctcctcg gccgcgattg gccggatgtc gacgtccatg cgcccgttgt
      541 acccggtcgc gcccttgggc gcacgccgtt ttcgacccgc tccccgcagc cgcaggcgta
      601 ctcgtagatc ggcacggtca ccagttcgcc atcgaggacc tgaagatccc ggccaggtcc
      661 tcctcggtgg cgtcgcgggg cgcgatcacg agcagccgcc gctgcttcat cgtcccggcg
      721 accagctcgt cgacgtcgtc ctcgccgtag ccgagcgccc cgatcccgtt cggggtgccg
      781 atgtcgcgca tcagcccgac gagcgcgtcc gggagcagct cgcgcgggtc gcgcccgccc
      841 gccgggtccg cgccgagcag ggtggcggcg cgcacgtgcc ggtcggggtc ggccgggaac
      901 gtgtaccgga acgaggccgg ggcggtcacc gccaccgact cgccgtgcgg caccatcggc
      961 tcgtcgccga tgtagccctc tggccggtag tcgcgcacca tcccggcgat cgggtaggcg
     1021 caggcgtgcg ggatgtgcac gccggcgttg ccgaacccca tgccggcgaa ggtggcggcc
     1081 agcgccatct gggagcgcgc ctcgaggtcg tggccgacga gcacggcccg ccggaacgag
     1141 cggttgatca tgccgagggc ccgctcggac cacatgtcgg agatcgggtt ggagccgcag
     1201 taggcgaccc ggtcggccgc gtccttgcgg gggtagctgg tgtagcgccg cgccgtgtag
     1261 gactcgaggg cgtggccgac gatgtccatc ccgctcgccg cggtcgcggt cggcggcagc
     1321 gtcagcgtca ccagcgggtc gatcacggcc agggtcgggc gcaggcgcgg gtggctgatg
     1381 ccgctcttga ccttgagccc gaggatgtcg agcacgcaga ccggcgtcga ctccgagccg
     1441 gtgccggccg tggtcggcac ggcgacgagc ggcttgagcg gccccgaggg cgccctgccc
     1501 tcgcccaccg gcttgttgac gtagtccatg acgtcgccgg gcgacgaggt catgaggttc
     1561 atggccttcg cggtgtcgat cgacgagccg ccgccgatcg cgaggaagcc gtcccattcg
     1621 cccgcccggg cgtactcggc ggcctcgcgc aggctctcgt cggtcggctc cacgtgcacg
     1681 ccatcgtaga tctcggcctt cagcccggcc tcctcggccg catggcgcgc ccgctccggg
     1741 atgccggtct cggcgacccc gcggtcggtg acgacgagca cccgctcgac gccgagctgg
     1801 gcgaggtcgt gccccagctc gtcgatggcg ccggtgccgt acttcagtgg cggcgccccc
     1861 caggtgaaga cggtctcagt ggacaactcg gccatgctcg ctcccccttc gggtagcagc
     1921 cccccgtgtg ccccggcgtc tcgagcagcg tcaccccggc agatctcgat gtcctgcagg
     1981 gtcagccgga tggaagcgag cccggcacgg ggccgctcga ggagttctgg tgcaacgcgc
     2041 tgtaggcact cgagagcgcc gcgcaggcgt gcgcgtaggt gatctcagga acgacccctg
     2101 ccagatgggc gtagggcctg tcatagaggg attcctgcag ctgggtgaca ccccggttgt
     2161 cgacgtggct cagggttttg cgggcttgct ctgcccagtg ctccgcctgg tcgcgggtct
     2221 cctcgagctt gcccatgcgg tgcctcctcg ctgagccggg gtgtcgttcg cctgcccatc
     2281 ctgcccgtgc gcctggtcct gttcgaggac ctcgaacagg atatcgccgt ggcagggctg
     2341 gtcgggtcgg caccagcacg cgagcgtctt gccggccagc tcgcggcggg cggcctcggc
     2401 cagttcagga tgttcggtga gatgacggcg gtagagcgcc aaggccccga ggaggccatg
     2461 ctcgctgacg cggtaggggt tgccccactt gctggggcgg gagatgacca ccgcgtcagg
     2521 aggcttgcgc catccgttgg tacgtcggag ctggacgcgg cgtggggcgg aggtggcgtc
     2581 gctggtgcgc atcggggcgc ctcctcgggc cggttgggtg gaagcgctgg gagctagccc
     2641 ttcagcttga cgatctgaat gccggagccg ccgctgatgc agacgaagga ggggctgcag
     2701 atgaacaggc cgatgctgac ggcagcgttg ccaccgtgga acggcgggcc cgagggatcg
     2761 gccaagaacg tgaaggtgtt cggttgcgcc gttccatcgc acacgatggg ctgatcggca
     2821 aatcccgagc cctgcgcgat ctcgtgcccc gcggcctgct cgatggtcgc gctctgttgc
     2881 gcggtgccca cctcgagcgg cccgcaggtg atctgcacgg gcaccgtgac cacgatgcgg
     2941 ctctgcaccg tgcccgttgc gccgaccgtg atggttacct ccggcgactg cgccttggcg
     3001 ctgagcggcg tcaacgctac gagggccgac acgcacgtca gcacgaaaag ccgccggagt
     3061 aatcgtctgg tcatgctcta
//
LOCUS       DASPYY010000359         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2589775, whole genome shotgun sequence.
ACCESSION   DASPYY010000359 DASPYY010000000
VERSION     DASPYY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589775"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VEP73_05520"
     CDS             <1..658
                     /locus_tag="VEP73_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT25924.1"
                     /translation="GVYAIANLRGGSEEGEAWHRAGMREHKQNVFNDFAAAAEHLVEA
                     GYTQPNRLAVYGGSNGGLLVGAALTQRPELFRAVVCSAPLLDMIRYEQFGLGETWNDE
                     YGRADDPAELRWLLGYSPYHHVAQGMAYPAVLFTVFDSDTRVDPLHARKLCAALQCAT
                     SSSPAERPVLLRRERDAGHGARSVRRTVELNADMLAFLADQLGLTVAAAPEPAAASPG
                     "
     gene            complement(666..1514)
                     /gene="trpA"
                     /locus_tag="VEP73_05525"
     CDS             complement(666..1514)
                     /gene="trpA"
                     /locus_tag="VEP73_05525"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYT25925.1"
                     /translation="MAEPEPPGQTLRPVDRLEGRLATLRAAGRAALVAYGVAGYPDRA
                     TSLAAFQAMAAAGADILEVGPPYSDPLIDGPVIQRAVQAALDAGTRLDDVFALVGELT
                     ASVDIPVVLLVYYNLVAHRGPARFAAETAAAGAGGAVVPDLPPEEAGEWLAAAEAAGI
                     APVFLAAPTSTEPRLEAVARAGRGFVYAQASLGVTGLRASLAAGIDELVARVRDHTER
                     PVCAGIGVSNAEQAATVARFADGVIVGTALVRCLGDGGVEGVRALTAELAEAVHGARQ
                     PAGAGG"
     gene            complement(1507..2832)
                     /gene="trpB"
                     /locus_tag="VEP73_05530"
     CDS             complement(1507..2832)
                     /gene="trpB"
                     /locus_tag="VEP73_05530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYT25926.1"
                     /translation="MSETSEPNHNRPDGQGVLLDLPPLRRPVPHPPDQLGHFGPYGGR
                     YVPEVLMAALDQLTDAFDEAMADPGFHAELDELRRSYAGRPTPLTPARRLSEELGLTV
                     WLKREDLLHTGAHKLNNSLGQQLLTRRMGKPRVIAETGAGQHGVATATAAALLGQECV
                     VYMGEEDTRRQHLNVVRMRLLGTEVRPVATGTATLKDAINEALRDWVESVGHTHYAIG
                     SVVGPHPFPLMVREFQRVIGEEARAQLLDAAGRLPDLAVACVGGGSNAAGLFAPFIDD
                     ATVQLVGVEAGGRGRGPGDNGAPLNFGQAGVLHGARSYLLQDPDGQVLPTHSVSAGLD
                     YPGVGPEHALWKDDGRVTYTEAGDRRALDGFQLLARTEGILPALEPAHAIGWLASAAR
                     AGGVAPGSVVVLNLSGRGDKDVETVASLLGMSSEGTEGEDAEGGERHRG"
     gene            complement(2825..4132)
                     /locus_tag="VEP73_05535"
     CDS             complement(2825..4132)
                     /locus_tag="VEP73_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HYT25927.1"
                     /translation="MAEPRKVLLSEDELPTAWYNIVPDLPVKPEPYLHPGTREPVTPD
                     LLTPLFPMALIGQEVSTDRWIDIPGPVLDTYRLWRPTPLIRALDLEQALGTPARIYYK
                     YEGGSPAGSHKPNTAVAQAYYNKAEGITRLSTETGAGQWGSALAFACARFGLECKVYM
                     VRASYDGKPYRRSLMQTWGASVTPSPSPETAAGRAILAQDPASPGSLGIAISEAVEVA
                     AQRDDTHYALGSVLNHVLLHQTVMGLELKAQLAKLDVVPDVLIGCVGGGSNWGGFSFP
                     LLADKLAGRIGTRFVAVEPTAAPTLTKGRFDYDLGDQAGMTPLIPMYTLGADFIPAPI
                     HAGGLRYHGDSPLISLLVRDGHMEAVAYPQTKVFDAAVQFARTEGILPAPEPAHCIRA
                     AVDEALRCKEQNSAETIVFNLCGHGHFDLKAYEDYLSGDLLDV"
     gene            complement(4132..4962)
                     /gene="trpC"
                     /locus_tag="VEP73_05540"
     CDS             complement(4132..4962)
                     /gene="trpC"
                     /locus_tag="VEP73_05540"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYT25928.1"
                     /translation="MADPAALSSFLARAVADAREDAERRAAKLPLDAIKEAATAAPPP
                     RGFRAALSQSKPGVALIAEIKRRSPSRGDIRPDLDPAALARAYQRGGAAAVSILTEPR
                     HFAGSPTDLRAAHAATDLPVLRKDFIITPYQVWEARAWGADALLLIVAALDPALLTAL
                     LTETAAAGLDALVEVHTRAEAETAAASGATLVGINARDLATLGVDPGRFAAVRDVLAG
                     EVTVVAESGIHDRAGVLAVASAGADAVLVGESLVRARDPAAAAAALLGRKQTVSSGGI
                     "
     gene            complement(4996..>6269)
                     /locus_tag="VEP73_05545"
     CDS             complement(4996..>6269)
                     /locus_tag="VEP73_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT25929.1"
                     /translation="SGTTVYLLNREGTPTLACPGRILARVSSAAPPPEVDLRGALNAG
                     SGQITSGTKGGLVYAVAPLSGSGANRAGVLLVHPARGLARSLLGPLAWRLLFATALAV
                     VIAALVAAALAGRLTAPLGRLVGAARDLGDGDLSTRVAVEGDDEVAEVGAAFNEMAAA
                     LEQSQAEQRAFLASVGHELKTPLTAVQGYTEALLDGTVEDPAARRRSLERIHAETLRL
                     ARLVQDLLDLARLGRGQFAVSPVDADAATVLREAAATWSERAAARGVTVELRLPGQLP
                     AHVDPGRLRQVLDNLLDNATRSSPAGRPVLLAAVQLPAGGAEVAVVDQGPGIAAEDLA
                     HVFDRGYLWSRYRGTRAVGSGLGLAIVKALCDAMQVTIWAESGGGGGTAFRLTLPPAH
                     TIPPPGRRPSQTQELPIPSSSASGPPPWEPA"
BASE COUNT          825 a         2071 c         2538 g          835 t
ORIGIN      
        1 tggcgtgtac gccatcgcga acctgcgggg cggcagcgag gagggcgagg cctggcaccg
       61 cgccggcatg cgggagcaca agcagaacgt gttcaacgac ttcgccgccg ccgcggagca
      121 cttggtcgag gctggctaca cgcagccgaa ccgcctggcg gtctacggcg ggtcgaacgg
      181 cgggctgctg gtcggggcgg cgctgacgca gcggccggag ctgttccggg cggtggtgtg
      241 cagcgcgccg ctgctggaca tgatccgcta tgagcagttc gggctgggtg agacctggaa
      301 cgacgagtac ggccgggccg acgaccctgc cgagctccgc tggctgctgg gctactcccc
      361 ctaccatcac gtggcgcaag gcatggcgta cccggcggtg ctgttcacgg tgttcgattc
      421 ggacacccgc gtcgacccgc tgcacgcccg caagctgtgc gcggcgctgc agtgtgcgac
      481 cagctccagc ccggcggagc gcccggtgct gctgcgccgc gaacgcgacg ccggccacgg
      541 ggcccgctcg gtgcgtcgca ccgtggagct gaacgccgac atgctggcgt tcctcgctga
      601 ccagctcggc ctcacggtgg ccgccgcgcc cgagccagcg gcagcgtcgc cgggttagcc
      661 gagggttagc cgccggcgcc ggcgggttgc cgggcgccgt ggacggcttc cgcgagttcg
      721 gcggtgaggg cgcggacgcc ctcgacgccg ccgtcgccga ggcagcggac cagggcagtg
      781 ccgacgatga cgccgtcggc gaagcgggcg acggtggcgg cctgctcggc gttggagacg
      841 ccgatgccgg cacacacggg ccgctcggtg tggtcgcgga cgcgggcgac gagctcgtcg
      901 atgccggcgg ccagcgaggc gcgcagcccg gtgacgccga gggaggcctg ggcgtagacg
      961 aagccgcgcc ctgcgcgggc gaccgcctcc agccgcggct cggtggaggt cggcgcggcg
     1021 aggaacaccg gggcgatccc ggcggcctcg gcggcggcga gccactcccc cgcctcctcc
     1081 ggcggcaggt cggggacgac cgcgccgccg gcgccagcgg cggcggtctc ggcggcgaag
     1141 cgggccgggc cgcggtgggc gacgaggttg tagtagacga gcaggaccac cgggatgtcg
     1201 accgaggcgg tcagctcgcc gaccagggcg aagacgtcgt ccagccgggt gccggcgtcg
     1261 agggccgcct gcaccgcgcg ctggatgacg ggtccgtcga tgagcggatc ggagtagggc
     1321 gggccgacct ccaggatgtc ggcacccgca gcggccatgg cctggaaggc ggccagcgag
     1381 gtggcccggt cgggataccc ggcgaccccg taggcgacca gcgcggcccg cccggcggca
     1441 cgcagtgtgg cgagccggcc ctccagccgg tcaacgggcc gaagcgtctg gcctggaggc
     1501 tcaggctcag ccacggtgcc gctcgcctcc ctcggcgtcc tcgccctcgg tgccctcaga
     1561 gctcatgccg aggaggcttg cgaccgtttc gacgtccttg tcaccccggc cggagaggtt
     1621 gagtacgacc acggacccag gggcgacgcc gcccgcgcgg gcggcgctgg cgagccagcc
     1681 gatcgcatgg gcgggctcca gggcggggag gatcccctcg gtgcgggcga gcagctggaa
     1741 gccgtcgagg gctctcctgt ccccggcctc ggtgtaggtg acacggccgt cgtccttcca
     1801 cagagcgtgc tcggggccga cgccgggata gtcgagccct gcggacaccg agtgggtggg
     1861 caggacctgc ccgtcggggt cctgcagcag gtaggagcgg gcgccgtgga ggacgccggc
     1921 ctggccgaag ttgagcgggg cgccgttgtc gccgggccca cgcccgcggc ctccggcttc
     1981 gacgccgacg agctggacgg tggcgtcgtc gatgaacggg gcgaacagcc cggcggcgtt
     2041 ggagccgccc ccgacgcagg cgaccgccag gtcgggcagg cgcccggcgg cgtcgagcag
     2101 ctgcgcgcga gcttcttccc cgatgacccg ctggaactcg cggaccatca gcgggaacgg
     2161 gtgcgggccg accaccgagc cgatggcgta gtgggtgtgg ccgaccgact cgacccagtc
     2221 cctgagcgcc tcgttgatgg cgtccttgag cgtggcggtg ccggtggcga ccgggcggac
     2281 ctcggtgccg agcaggcgca tgcgcaccac gttgaggtgc tggcggcggg tgtcctcctc
     2341 acccatatag acgacgcatt cctggccgag cagagcggcg gcggtggcgg tggcgacgcc
     2401 gtgctggccg gcgccggtct ccgcgatcac ccggggcttg cccatgcgtc tggtgagcag
     2461 ctgctggccg agggagttgt tgagcttgtg cgcgccggta tgcagcaggt cctcgcgttt
     2521 gagccagact gtcaggccga gctcctccga caggcgccgg gcgggggtga gcggagtggg
     2581 cctgccggcg taggagcgcc gcagctcgtc cagttcggcg tggaagccgg ggtcggccat
     2641 ggcctcgtcg aaggcgtcgg tgagctggtc gagggcggcc atgagcacct cgggcacgta
     2701 acggccgccg tagggaccga agtgcccgag ctggtcgggc gggtgcggga cgggacggcg
     2761 cagcgggggc aggtccagca ggacgccctg cccgtcgggc cggttgtggt tcggttcaga
     2821 cgtctcagac atcgagcagg tcccctgaca ggtagtcctc gtaggccttg aggtcgaaat
     2881 gcccgtggcc gcagaggttg aagacgatgg tctcggccga gttctgctcc ttgcagcgca
     2941 gcgcctcgtc gacggcggcg cggatgcagt gggctggttc cggtgctggc aggatgccct
     3001 cggtgcgggc gaactggacg gcggcgtcga agaccttggt ctgcgggtag gcgaccgcct
     3061 ccatgtgccc gtccctgacc agcagggaga tcagcggcga gtcgccgtgg tagcgcagcc
     3121 ctccggcgtg gatcggggcg gggatgaagt ccgccccgag cgtgtacatc gggatcagcg
     3181 gggtcatccc ggcctggtcg ccgaggtcgt agtcgaagcg gcccttggtg agggtgggcg
     3241 cggcggtcgg ctcgacggcg acgaaacggg tgccgatgcg gccggcgagc ttgtcggcca
     3301 gcagcgggaa cgagaacccg ccccagttcg acccgccgcc gacgcagcca atcaggacgt
     3361 ccgggaccac gtcgagcttg gcgagctggg ccttcagctc caggcccatg acggtttggt
     3421 gcagcagcac gtggttgagg acgctgccga gcgcgtagtg cgtgtcgtcg cgctgggcgg
     3481 cgacctcgac ggcctcggag atggcgatgc cgagcgagcc gggcgaggcg gggtcctggg
     3541 cgaggatggc gcggccggcg gcggtctcgg gtgagggtga gggggtgacc gacgcgcccc
     3601 aggtctgcat cagcgaacgc cggtacggtt tgccgtcgta ggaggcccgc accatataga
     3661 ccttgcactc caggccgaaa cgggcgcagg cgaaggcgag cgcgctgccc cactggccgg
     3721 ctcctgtctc ggtagagagg cgagtgatgc cttcggcctt gttgtagtag gcctgcgcga
     3781 cggcggtgtt gggcttgtgg gagccggccg gggagccgcc ttcgtacttg tagtagatcc
     3841 gggccggagt gccgagggct tgttccaggt cgagggcgcg gatcaacggg gtgggccgcc
     3901 acagccggta ggtgtcgagc accgggcctg ggatgtcgat ccagcggtcg gtggagacct
     3961 cctggccgat cagggccatg gggaacaggg gtgtgagcag gtccggagtg accggctccc
     4021 gggtccccgg gtgcaggtag ggctcgggct tgacgggcag gtccgggacg atgttgtacc
     4081 aagccgtagg cagctcgtcc tcgcttagca ggaccttacg cggttcagcc atcagatacc
     4141 tcctgaggag actgtctgct tgcggccgag cagggcggcg gcggctgcgg ccgggtctct
     4201 ggcgcggacc agggattcgc cgaccaggac agcatcggct ccggcgctgg ccacggcaag
     4261 gacgccggcg cggtcgtgga tgccgctctc ggcgaccacc gtgacctccc cggccaggac
     4321 gtcgcggacg gcggcgaaac ggcccgggtc gacgccgagg gtggcgaggt cgcgggcgtt
     4381 gatgccgacc agggtcgcgc cggacgcggc ggcggtctcg gcctcggcgc gggtgtggac
     4441 ctcgaccagg gcgtccagtc cggccgcggc ggtctcggtg agcagggcgg tgagcagggc
     4501 ggggtcgagc gcggcgacga tcagcagcag cgcgtccgcg ccccaggcgc gtgcctccca
     4561 gacctggtag ggggtgatga tgaagtcctt gcgcagtacc ggcaggtcgg tggctgcgtg
     4621 ggcggcccgc aggtctgtgg ggctgccggc gaagtggcgc ggttcggtga ggatgctgac
     4681 ggcggcggcg ccgccgcgct ggtaggcgcg ggcgagcgcg gccgggtcca gatccggacg
     4741 gatgtctcct ctggagggcg agcgccgctt gatctcagcg atcaaggcca caccaggctt
     4801 cgactgcgac aaggcggcac ggaaaccgcg cggcgggggt gcggcggtgg ccgcctcctt
     4861 gatcgcgtcg agaggaagct tcgccgcacg ccgctcggcg tcctcgcggg cgtcggcgac
     4921 cgcacgggca aggaacgagg aaagcgcagc aggatcagcc atcgcccgat tctaccggcg
     4981 ctgccggtca gcgactcaag cgggttccca gggcggcggc ccgctggcgc tggacgaggg
     5041 aatgggcagc tcctgggtct gcgatggccg ccgccctggc ggggggatgg tgtgcgctgg
     5101 tgggagggtg aggcggaaag cggtcccgcc accgccgccc gattccgccc agattgtcac
     5161 ctgcatggcg tcgcagagcg ccttgacgat ggccaggccg aggccggagc cgaccgcgcg
     5221 ggtgccgcgg tagcgggacc acaggtagcc ccggtcgaac acgtgcgcca ggtcctctgc
     5281 tgcgatgccg ggaccctggt cgaccacggc gacctcggcg ccaccggcgg ggagctgcac
     5341 ggcggcgagc agcaccgggc ggccggcggg gctggagcgg gtggcgttgt cgagtaggtt
     5401 gtcgagcacc tggcgtaggc gcccggggtc gacgtgggcg gggagctggc cgggcaggcg
     5461 cagctcgacg gtgacgccgc gcgcggccgc gcgctcgctc caggtggccg ccgcctcgcg
     5521 cagcacggtg gcggcgtcgg cgtcgaccgg gctgaccgcg aactggccgc ggccgagccg
     5581 agccaggtcg agcaggtcct ggaccaggcg ggcgaggcgc agggtctcgg cgtggatccg
     5641 ctctaggctg cggcgccgcg cggccgggtc ctcgacggtg ccgtccagca gcgcctcggt
     5701 gtagccctgt accgccgtca ggggggtctt cagctcgtgg ccgacgctgg cgaggaaggc
     5761 gcgctgctcg gcttggctct gctccagcgc ggcggccatc tcgttgaagg cggcgccgac
     5821 ctcggcgacc tcgtcgtcgc cctcgaccgc gacccgggtg gagaggtcgc cgtcgccgag
     5881 gtcgcgcgcg gcgccgacca gccgcccgag cggcgcggtg agccgcccgg cgagcgcggc
     5941 ggcgaccagc gccgcgatca ccacggccaa ggcggtggcg aacagcagcc gccaggcgag
     6001 cggcccgagc aggctgcgag cgagccccct ggcggggtgg accagcagca ccccggcgcg
     6061 gttggcgccg ctgccggaca gcggcgcgac cgcgtagacc aagccgccct tggtgccgct
     6121 ggtgatctgg ccgctgccag cgttgagggc gccgcgcagg tccacctccg gagggggcgc
     6181 tgcgctgctc acgcgggcga ggatccgccc tggacaggcg agcgtcgggg tcccttcgcg
     6241 gttgagcaga tacaccgtgg tcccactca
//
LOCUS       DASPYY010000360         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_61087, whole genome shotgun sequence.
ACCESSION   DASPYY010000360 DASPYY010000000
VERSION     DASPYY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61087"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VEP73_05550"
     CDS             <1..1204
                     /locus_tag="VEP73_05550"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25930.1"
                     /translation="EVRVPIEDELPGFFDQLSRVFSGRPQEAARLSRNLSLTELDRTL
                     VASEGRQIVGTAGVFSLRMTTPGGGDVPTAGVTRVSVAPTHRRQGLLRALMRRQLDDV
                     HARGEPLAALYASQAPIYGRFGYGVGTWHSHLVIPRARSAFRDPPGGGRVRFLEAAAA
                     LEALPDVYERIRREQPGAVTRSQAYWQARLTDPPDPRQDRGPLWFLLHEGDRGPDGFA
                     VYQVQARWQQEDPNGTLFVIDLLGANPVATAALWRFCLDVDLMARVEAAARPAEDPVR
                     HLLADTRAARVTVYDGLWVRLVDVGAALGCRSYAAAGPLTIEVRDDFCPWNAGRWCLE
                     GGECRRSDADPDLVLDVADLAAVYLGGERWATLAAAGRVEERAPGSLKRADAIFASSP
                     APWCPTHF"
     gene            1260..>1654
                     /locus_tag="VEP73_05555"
     CDS             1260..>1654
                     /locus_tag="VEP73_05555"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HYT25931.1"
                     /translation="MEKLLLELSGAAHPRLCFIPTAGADSDYGIVRFHEVFGRHARVT
                     HLPLFRRQGDVREVLLDQDAIWVGGGNTLNMLAVWRLHGVDAALREAWERGIVLGGAS
                     AGSICWFVSGVTDSYGPELKPITGCLGWLP"
BASE COUNT          189 a          631 c          594 g          240 t
ORIGIN      
        1 cgaggtccgg gtccccatcg aggacgagct gcccggcttt ttcgaccagc tgagccgcgt
       61 cttctctgga cgcccgcagg aggcggcgcg cctgtcgcgc aacctttcgc tgaccgagct
      121 cgaccgcacg ctggtcgcgt ccgagggacg ccagatcgtg ggcacggccg gcgtcttcag
      181 cctgcgcatg accactcccg gtggcggcga cgtacccacc gccggcgtga cgcgggtctc
      241 ggtggcgccc acacaccgcc ggcaggggct gctgcgcgcc ctgatgcggc gccagctcga
      301 cgacgtccac gctcgcggcg agccgctggc cgccctctac gcctcgcagg cgccgatcta
      361 cggtcgcttc ggttatggcg tcggcacctg gcacagccac ctggtgatcc cgcgggcgcg
      421 ctcagccttc cgggaccccc ccgggggcgg ccgggtccgg ttcctggagg cggcggccgc
      481 gctggaggcg ctgcccgacg tctacgagcg catccggcgc gagcagcccg gcgccgtcac
      541 ccgctcgcag gcctactggc aggcgcgcct gacggacccg cccgacccgc gccaggaccg
      601 cggcccgctc tggttcctgc tccacgaggg cgaccgcggc cccgacggct tcgcggtcta
      661 tcaggtccag gcccgctggc agcaggagga ccccaacggg actctgttcg tgatcgacct
      721 gctgggcgcc aacccggtgg cgacggccgc actctggcgg ttctgcctgg acgtcgacct
      781 gatggctcgc gtggaggcgg ccgcccgccc ggcggaggac ccggttcgcc acctgctggc
      841 cgacacgcgg gcggcgcggg tcaccgtcta cgacgggctc tgggtgcggc tggtcgacgt
      901 cggggccgcc ctcggctgcc gctcctatgc cgcggcgggc ccgctcacga tcgaggtccg
      961 ggacgacttc tgcccctgga acgcgggccg ctggtgtctg gagggcgggg agtgccggcg
     1021 gtcggacgcg gaccccgacc tagtgctcga cgtggccgac ctggcggccg tctacctggg
     1081 cggcgagcgc tgggccacgc tggccgccgc cggccgcgtc gaggagcgcg cgccgggatc
     1141 gctgaagcgg gctgacgcca tcttcgccag cagcccggca ccctggtgcc ccacccattt
     1201 ctgatcccac gatcctggcc gcgggcggcg gcagcttctt cgtcgatccc agcgcgccca
     1261 tggagaagct gctgctcgag ctgagcggcg ccgcacaccc gcggctgtgc ttcatcccga
     1321 cggccggcgc cgactccgat tacggcatcg tccgcttcca cgaggtgttc ggccgccacg
     1381 cgcgagtgac ccacctgccg ctcttccggc gccagggcga tgtccgcgag gtgctgttgg
     1441 accaggacgc gatctgggtc ggcggcggca acaccctcaa catgctcgcc gtctggcgcc
     1501 tgcacggggt cgacgccgcc ctgcgcgaag cctgggagcg ggggatcgtc ctgggcggcg
     1561 ccagcgcagg ctcgatctgc tggttcgtgt cgggcgtcac cgactcctac ggccccgagc
     1621 tcaagccgat caccggctgc ctgggctggc ttcc
//
LOCUS       DASPYY010000361         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2168613, whole genome shotgun sequence.
ACCESSION   DASPYY010000361 DASPYY010000000
VERSION     DASPYY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168613"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VEP73_05560"
     CDS             <1..311
                     /locus_tag="VEP73_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT25932.1"
                     /translation="KLSDAIAFDEHQMAKQRLFETDRTLTDVYCLDPGQEQLLRVHEA
                     SDKVVLVMAGRVLVRVDDDTAELGQYEAVLAPAGSKHALKNLGPDPAVLVVFVGPRLA
                     "
     gene            complement(327..1709)
                     /locus_tag="VEP73_05565"
     CDS             complement(327..1709)
                     /locus_tag="VEP73_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HYT25933.1"
                     /translation="MRATREEPPRRGAALPEWGVDPVPGDWRVLGFLDQAVLWGNLGI
                     SLLVLVAGALLVPALGLWQALAATVVGAAVGNALLGLAAVPAADTGVPTMVLYRAPLG
                     VRGSLLPTACNVVQNLGWATFELFIIASAAAQVSERLFGFRGRLLWVVVFGALTTALA
                     VAGPLTVVRRYLERFAVWAVLASTAYLTWHALTAFDLSRIAARPGSGGLSFWAGVDIA
                     VALPVSWVPLVADYARFGRSARATFWGTSAGYFVAQVWFYALGILFLLAIGHGDVIAA
                     VMAVPVGVLAMAVLVVDEADEVFANVYSTGVSLQNAAPRLPGRPLALVLGVLATALAA
                     LVGDLSRYESFLLLLGALFVPLFGVLAADYFVLARRNYDVAAMYEPAGRYRGVRWPAV
                     LVWLGGFLIYEWINPGTVATWSSLLRGLFADLLHVPFPLSGRLPWLGASVPAFVLPFL
                     AMIAVGRRRR"
     regulatory      complement(1714..1801)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1889..>2426)
                     /locus_tag="VEP73_05570"
     CDS             complement(1889..>2426)
                     /locus_tag="VEP73_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25934.1"
                     /translation="DLDDLLGEPVYLGGGDDQGVARDHAGRDDQVELAEPTPTPDPGS
                     LPTTGPTSGTSAWAAGDEPAAPAGPDEPLTPATGAATVPQPTSTSAALLESVDAEAIR
                     RQFLDIQAGFVDEPRQAVQQAGELVDDLVHKVLESLTAEREQLVTGMGADTASTEDLR
                     LTLRAYRVYVDRLLGLTM"
BASE COUNT          373 a          880 c          921 g          252 t
ORIGIN      
        1 ggaagctctc cgacgcgatc gcgttcgacg agcatcagat ggccaagcag cggctgttcg
       61 agaccgaccg caccctcacc gacgtgtact gcctggaccc cgggcaggag cagctcctcc
      121 gcgtccacga ggcgtccgac aaggtggtcc tggtgatggc cggccgggtc ctcgtgcggg
      181 tggacgacga caccgccgag ctcggccagt acgaggccgt cctcgccccg gcgggatcca
      241 agcacgcgct gaagaacctc gggcccgacc cggcggtgct ggtggtgttc gtcggcccac
      301 gcctcgcgta gcgcgggcct ccgcgctcac cgccggcgcc gcccgacggc gatcatggcg
      361 aggaagggca ggacgaacgc aggcaccgac gcgccgagcc agggcaggcg gcccgacagc
      421 gggaacggca cgtgcagcag gtcggcgaac aggcccctga gcagcgacga ccaggtggcg
      481 accgtgcccg ggttgatcca ctcgtaaatc aggaagccgc cgagccagac gagcaccgcc
      541 ggccagcgca cgccccggta gcggccggcc ggctcgtaca tggcggcgac gtcgtagttg
      601 cggcgggcga gcacgaagta gtcggccgcg aggaccccga acagcggcac gaagagcgcc
      661 ccgagcagca ggaggaagct ctcgtagcgg gagaggtcgc cgaccagcgc cgcgagcgcc
      721 gtggcgagga cgccgaggac gagggcgagc ggccggccgg ggagccgcgg ggcggcgttc
      781 tgcaggctca ccccggtcga gtacacgttg gcgaacacct cgtctgcctc gtcgacgacg
      841 agcacggcca tggcgagcac cccgaccggc accgccatga cggcggcgat cacgtcgccg
      901 tgcccgatgg cgagcaggaa caggatgccg agggcgtaga accagacctg cgccacgaag
      961 tacccggcgc tcgtgcccca gaaggtcgcg cgggccgagc gcccgaagcg ggcgtagtcg
     1021 gccacgagcg gcacccagga gaccggcagg gcgacggcga tgtcgacccc ggcccagaag
     1081 gacaggccgc cgctcccggg ccgggcggcg atacgggaga ggtcgaaggc cgtgagcgcg
     1141 tgccaggtca ggtaggcggt ggaggcgagc accgcccaga ccgcgaaccg ctcgaggtag
     1201 cgcctgacga cggtgagcgg cccggcaacc gcgagcgcgg tggtcagcgc gccgaacacc
     1261 acgacccaga gcaggcggcc gcggaacccg aacaggcgct cggagacctg ggcggcggcg
     1321 ctggcgatga tgaacagctc gaaggtggcc cagcccaggt tctggacgac gttgcacgcg
     1381 gtgggcagga gcgagccgcg caccccgagc ggcgcccggt acagcaccat cgtgggcacg
     1441 ccggtgtcgg cggcgggcac ggcggcgagc ccgagcaggg cgttgccgac ggcggcgccg
     1501 acgacggtcg cggccagcgc ctgccagagg ccgagcgcgg gcacgagcag cgccccggcc
     1561 acgagcacga gcaggctgat gccgaggttg ccccacagca ccgcctggtc gaggaacccc
     1621 agcacccgcc agtcgccggg caccgggtcg acgccccact ccggcagcgc cgccccccgt
     1681 cgcggcggct cctcgcgggt tgctcgcatc gcccctccct gcgccggtat gagccggatc
     1741 aggttcgaag ggtcgccgac tccgcgtcgg cctctcagcc cggcgcaccg gactcccctg
     1801 gcctcacccc tgcacgcgct cgccacgtgc ggcgcagcga agatagccct cccgccgccc
     1861 ggcgacaagg agggcttctt ccggccccct acatcgtgag gccgagcagg cggtcgacgt
     1921 agacccggta ggcacgcagc gtcagccgca ggtcctcggt cgaggccgtg tcggcgccca
     1981 tgcccgtcac gagctgctcg cgctcggccg tgagggactc gagcaccttg tggacgagat
     2041 cgtcgaccag ctccccggcc tgctggacgg cctgcctggg ctcgtcgacg aagccggcct
     2101 ggatgtcgag gaactgccgc cggatggcct cggcgtcgac cgactcgagc agggcggccg
     2161 acgtcgaggt cggctgcggc acggtcgcgg cgccggtcgc cggggtgagc ggctcgtcgg
     2221 ggcccgcggg cgcggccggc tcgtccccgg ccgcccaggc ggacgtcccg ctggtcgggc
     2281 cggtggtggg cagcgagccc gggtcgggcg tcggcgtagg ctcggccagc tccacctggt
     2341 cgtcacggcc ggcgtggtcg cgggcaacgc cctggtcgtc gccgccgccg aggtacaccg
     2401 gctcgccgag gaggtcgtcg aggtcc
//
LOCUS       DASPYY010000362         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2309992, whole genome shotgun sequence.
ACCESSION   DASPYY010000362 DASPYY010000000
VERSION     DASPYY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309992"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VEP73_05575"
     CDS             <1..357
                     /locus_tag="VEP73_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HYT25935.1"
                     /translation="HEGEKMSKSLGNLVFPRELFREHEPAAVRLVLLAHHWRSAWELD
                     PDELKQAAERLETWRRAAAGSRGPARALPELVEAALAADLDTPAALRAVDALARDGAS
                     VAAAAEVLGVDLRPAR"
     gene            469..753
                     /locus_tag="VEP73_05580"
     CDS             469..753
                     /locus_tag="VEP73_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25936.1"
                     /translation="MRGDWVEVLVDTGNGVRAFEIAATRDGRRVEVTTSRSMVEVAEV
                     TRTGQTVRTARFLSGRVVAIVEHPATDGRQPRGAPDQDQAGHQAGRRPAT"
     gene            complement(770..997)
                     /locus_tag="VEP73_05585"
     CDS             complement(770..997)
                     /locus_tag="VEP73_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25937.1"
                     /translation="MPATDTTNPTRAALEAAVLDLTDEEIVEALTRIRSSSIKRAYED
                     RAGYAWPREAQRNALDALARRLAGGRVRAQG"
     gene            complement(1060..1836)
                     /locus_tag="VEP73_05590"
     CDS             complement(1060..1836)
                     /locus_tag="VEP73_05590"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015626247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HYT25938.1"
                     /translation="MQRIGVHLGRDAALAAAVERGADCVQFFLGDPQGWKVPPPGPDT
                     LELAASPVAVYVHAPYVINLASGNNRIRIPSRKLLAQTCEGAAAVGAAGVIVHGGHVT
                     ADDDEREGFARWRKALEQLESPVPLLLENTAGGDHAMARRLVTLARLWERVGDLGLGF
                     CLDTCHAHAAGEDLDGIADRVKAITGRIDLVHANDSKDPAGSGRDRHENLGHGQIDLD
                     TLVGVVQAAGAPVVCETPGGAADHRADLELLRGRLGGQGG"
     gene            1890..>2075
                     /locus_tag="VEP73_05595"
     CDS             1890..>2075
                     /locus_tag="VEP73_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25939.1"
                     /translation="MLRRALSSSLAVAVGLACLVAPGVADALLPGAREARDGRTALSA
                     NLSGRRETVPGDRDGVAR"
BASE COUNT          246 a          768 c          794 g          267 t
ORIGIN      
        1 cacgagggcg agaagatgtc caagtcgctc ggcaacctgg tgttcccgcg cgagctgttc
       61 cgcgagcacg agccggccgc ggtccgcctc gtgctgctcg cccaccactg gcgctcggcg
      121 tgggagctgg acccggacga gctgaagcag gccgccgagc ggctcgagac ctggcgccgg
      181 gcggccgccg gatcccgggg tccggccaga gcactccccg agctggtgga ggcggccctc
      241 gccgccgacc tcgacactcc ggccgccctg cgggcggtcg acgccctcgc ccgcgacggc
      301 gcgtccgtcg cggccgccgc cgaggtgctc ggcgtcgacc tccgcccggc tcgctgaacc
      361 ttgggcgccg gccagcggag tgtcatgctg gccaggtggc acaggccgta cggtgtcacc
      421 cgtcagcgca ggcagacacc gacgcgtcgg gaacggtagg ctgacgatat gcgtggagac
      481 tgggtcgagg tgctggtgga caccggcaac ggtgtgcgcg ccttcgagat cgcggcgacc
      541 cgcgacggcc gccgggtcga ggtgaccacc tctcggagca tggtggaggt ggccgaggtg
      601 acccggaccg gccagacggt ccgcacggcc cggttcctct ccggccgggt ggtcgcgatc
      661 gtcgagcacc cggcaaccga cggccgccag ccccgcggcg cccccgacca ggaccaggcc
      721 ggccaccagg ccggccggcg ccccgccacc tgaacgggat ccgacccggt cagccctgcg
      781 cccggacgcg cccgcccgcc agccgcctgg cgagcgcgtc gagcgcgttg cgctgggcct
      841 ccctgggcca ggcgtagccg gcccggtcct cgtacgcacg cttgatcgag ctgctcctga
      901 tcctggtcag ggcctcgacg atctcctcgt cggtcaggtc gaggaccgcg gcctcgaggg
      961 cggccctcgt cgggttcgtg gtgtcggtgg ctggcatcgg tcctccgcct cggcgcgcgt
     1021 gctcggcgcg agaaggatag gaccgctggc cgggcggggc tacccgccct gcccgcccaa
     1081 ccggccgcgg agcagctcga ggtcggcgcg gtggtcggcc gcgccgcccg gcgtctcgca
     1141 gacgaccggc gcgccggccg cctggaccac gccgaccagc gtgtcgaggt cgatctggcc
     1201 gtggccgagg ttctcgtgcc tgtcgcggcc ggacccggcc gggtccttgg agtcgttggc
     1261 atgcaccagg tcgatgcgcc cggtgatcgc cttgacccgg tcggcgatgc cgtcgaggtc
     1321 ctcgccggcg gcgtgggcgt ggcaggtgtc gaggcagaac ccgaggccga ggtcgcccac
     1381 ccgctcccac agccgggcca gggtgacgag ccggcgggcc atcgcgtggt ccccgcccgc
     1441 ggtgttctcg agcaggagcg gcaccggcga ctcgagctgc tcgagcgcct tgcgccagcg
     1501 ggcgaagccc tcgcgctcgt cgtcgtcggc ggtgacgtgc ccgccgtgca cgatcacgcc
     1561 ggcggcgccc accgcggccg cgccctcgca ggtctgggcg agcagcttgc gggacgggat
     1621 gcggatgcgg ttgttgccgg aggccaggtt gatgacgtag ggcgcatgga cgtacaccgc
     1681 gaccggcgac gcggccagct ccagggtgtc ggggcctggg ggcggcacct tccatccctg
     1741 cgggtcgccg aggaagaact ggacgcagtc ggcgccgcgc tcgacggcgg cggcgagcgc
     1801 ggcgtcccgt ccgaggtgga ccccgatcct ctgcatgccg agaagactag cagccgcgcg
     1861 cgacgtgccg ccgacaagag ggaggaccca tgctccgacg tgcgctgtct tcgagcctgg
     1921 cggtcgccgt cgggctcgcc tgcctggtcg cgcccggcgt ggccgacgct ctcctgcctg
     1981 gagcgcgaga ggccagggat gggcgcaccg cgctctcggc caacctctcg ggccgccggg
     2041 agacggtgcc gggcgaccgg gacggcgtcg cccgt
//
LOCUS       DASPYY010000363         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1187077, whole genome shotgun sequence.
ACCESSION   DASPYY010000363 DASPYY010000000
VERSION     DASPYY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187077"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1100)
                     /locus_tag="VEP73_05600"
     CDS             complement(90..1100)
                     /locus_tag="VEP73_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT25940.1"
                     /translation="MSQPRKRVTIQQVAEAVGLSTAAVSYALRGSHVSKETEERVRRV
                     ASELGYEAHPIARALASGRTSMVGVISGSLEDLWQQQVSVGIGRGLLARDLYALIVDA
                     GGDPEREQALALQLRDQRVDGLILSPLDPSAPLWAELAETLPVVAIGDSLQGAQPTGE
                     VLFDNRAGVTLALEHLHRLGHRRIAVLTSTQPSTPDRPADTYVLAEARRLGLDVAVRA
                     APHALPEATKVARKLLSTDGAPTALFCFSDSIAYGGYAAARDLSLRIPEDLSITGYDN
                     HPVSALLSPPLTTFDWDREGIIRTAVQLVVAAIDGRPRRRRVVKTPKLHLRGSTGPPP
                     RP"
     gene            1197..2123
                     /locus_tag="VEP73_05605"
     CDS             1197..2123
                     /locus_tag="VEP73_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYT25941.1"
                     /translation="MRVDVHQHLWPDAFVDALRSRRRAPRMAGWTLVLDGEPDFAVDP
                     ADHDVARRQAQASEDGLDLALVSPSSPLGTEWLPPEEAWPLLDAYHGGALALPTPFLP
                     WASACLSEIDPAQVGKELGRGCVGLQLPATALLDARGYERCAPLLDLLETHDRPLFVH
                     PGAAPAPAGPAPAWWPALVPYVQQMHAAWFAFRAFGRPRHPRLRVCFAMLAGLAPLHG
                     ERLRARGGDRGTADPAVARGAVDPAVFVESSSYGTRAVDATVRVLGVDAVVLGSDRPY
                     ASPADPGIGAAAAHAIRATNPTRLLDLEGVMG"
     gene            2120..2617
                     /locus_tag="VEP73_05610"
     CDS             2120..2617
                     /locus_tag="VEP73_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HYT25942.1"
                     /translation="MTWETLPARVLDKHELRALVDDLAARPELWSGHVAFSDERRHYV
                     SLHRDEHVDVWLLCWTRTNDTGWHDHDVSSGAVHVVRGAIAECNPRLGGEHVVTVVSE
                     GRSFCFGPDHIHRMSGEREGGVTIHAYSPPLWRLGQYAFGTDGVMRRVSVSYAEELRP
                     LDPAA"
     gene            2747..5056
                     /locus_tag="VEP73_05615"
     CDS             2747..5056
                     /locus_tag="VEP73_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HYT25943.1"
                     /translation="MAITLLPPGRATGTFLDAPPHHDPSTVASSFAVVGVPCAAPPGS
                     HADGGAPPDACDAPAVLRRRSQRFAGSRSHDDGDGDLLGGPVSLVDCGDVPSDPHDPS
                     RQEEHATRAVRALLGRGVVPVVLGGDRSVPAGVLAAYQGSGPLQAIAIGAHPRSREEA
                     GGLLVDAAGVRVHGVDWLLGRLPEDGRFLVTLDVDGLDPSIAPGTDRPREGGLLFHEA
                     AALLRGLAARGGVAGVCVVGLFPSLDVDQTTADTVVRLLASLMRAAGRKAGTAPVLLD
                     GERLRIVDVERIAGGTAAVEVPEAAWQRIARGRAVVEPLADRRVRAYGVTTGVGSHKD
                     YMVEPERLEEFNLRLLYAHATRSPGPVAPAEVVRACMAILVNLFASGRSGVRPALVER
                     LLADLREDRLPAVDLGSSVGASDLVALAQLALPLVGGGELLDGGELGPEERPPGAPVF
                     PAPKEALALVNNNSLTLGRGALVLAEARRLLVAMDIAAALALEGFRGNPRAWSAVVEQ
                     AHPQPGHRWAGERLRRLLAGSRLWRPGEPRFLQDPLSFRCVPQVSGAAREALAWTWRT
                     WERELNATVDNPLVDLASGEVVTHGSMETTLLAVTMDALRLTLAKALRTSAERTHKLH
                     WSSFSGLPMGLAAEAGASGGAQFLNLSYIVAAHVATSSIAATPVTPHYHGQLAEGVED
                     IAGLAPHAVTGAERLLQSAWVVVGIEALVASWAVARRGLAPADLGTALRPAHQMLLPL
                     VPAGREGERQLDVSPAVEALRSLARTWTE"
BASE COUNT          595 a         1953 c         1918 g          663 t
ORIGIN      
        1 tcgcggtcct cgatcccggg ctcgccgggg cggcgcccgg gtcctgaccc gcccgtgccg
       61 ggacccggcc cggtccgtcc tcgcgccggt caggggcgag gcggcgggcc ggtggacccc
      121 cgcaggtgca gcttgggcgt cttgaccacc cgtcgccgcc gcggcctgcc gtcgatggcg
      181 gcgacgacca gctggaccgc cgtgcggatg atcccctcac ggtcccagtc gaaggtcgtg
      241 agcggcgggg acagcagggc cgagaccggg tggttgtcgt acccggtgat cgacaggtcc
      301 tcggggatcc gcagggagag gtcccgggcc gccgcatagc cgccgtaggc gatcgagtcg
      361 gagaagcaga acagcgcggt gggcgccccg tccgtggaga gcagcttcct ggccaccttg
      421 gtggcctcgg ggagggcatg gggcgcggcc cgaacggcga cgtcgagacc gagccgtcgc
      481 gcttcggcca gcacgtaggt gtccgccggg cggtccgggg tgctcggctg ggtcgaggtc
      541 agcaccgcga tccgccggtg gcccagccgg tgcaggtgct ccagcgccag ggtcacgccg
      601 gcccggttgt cgaacagcac ctcgccggtc ggctgggcgc cctgtaggga gtccccgatg
      661 gcgaccacgg gcagggtctc ggccagctcc gcccagagcg gcgcggaggg gtcgagcggg
      721 gacaggatga gaccgtcgac cctctggtcg cgcagctgca gcgccagtgc ctgctcgcgc
      781 tccgggtcgc caccggcgtc cacgatgagc gcgtacaggt cgcgggcgag cagcccgcga
      841 ccgatgccga cgctcacctg ctgctgccag aggtcctcca gcgagcccga gatcactccc
      901 accatgctcg tccgcccgct cgccagcgcg cgggcgatcg ggtgcgcctc gtagcccagc
      961 tcgctcgcca cccgccggac acgctcctcg gtctccttgg agacgtggct cccgcgcagg
     1021 gcgtaggaga cggcggcggt cgacagcccg acggcctcgg cgacctgctg gatcgtcacc
     1081 cgcttgcgtg gctgtgacat ggagacaggc tacccgagac ggccgacctt gacgccgcca
     1141 tggttaagcg gttcactgaa gcggttcact gaagcgctta acctcgggac ggccgcatgc
     1201 gcgtcgatgt ccatcagcac ctctggcccg acgcgttcgt cgatgcgttg cgcagccgtc
     1261 ggcgcgcccc gaggatggcc ggctggacgc tcgtcctgga cggcgagccg gacttcgccg
     1321 tcgacccggc cgatcacgac gtggcgcgcc gccaggccca ggcgagcgag gatgggctcg
     1381 acctcgccct ggtgtccccg tccagcccgc tcggcaccga gtggcttccg cccgaggagg
     1441 catggccgct gctggacgcc taccacggcg gcgcgctcgc gctgcccacg ccgttcctgc
     1501 cctgggcgtc ggcctgcctg agcgagatcg accccgccca ggtcggcaag gagctcggcc
     1561 gcggctgtgt cgggctgcag ctgcccgcga ccgcgctgct cgacgcgcga ggctacgagc
     1621 ggtgcgcgcc cctgctcgac ctgctcgaga cccacgaccg gccgctcttc gtccacccgg
     1681 gcgccgcccc ggcgccggcg ggcccggccc ctgcctggtg gcccgcgctg gtgccgtacg
     1741 tgcagcagat gcacgccgcc tggttcgcct tccgggcctt cggccggccg cgccacccgc
     1801 ggctgcgggt ctgcttcgcg atgctcgccg gcctcgcccc cctgcacggc gagcgcctgc
     1861 gggcgcgcgg cggcgaccgg ggcacggcgg acccggcggt ggcccggggc gcggtggacc
     1921 cggcggtctt cgtcgagagc tcgtcctatg gcacgcgcgc ggtcgacgcc acggtccgcg
     1981 tcctcggcgt cgacgctgtc gtgctcggct cggaccggcc gtacgcgtcc cccgccgacc
     2041 ccggcatcgg cgcggcggcc gcgcacgcca tccgcgccac gaacccgacc cggctgctcg
     2101 acctcgaagg agtcatggga tgacctggga gaccctgcca gcgcgagtcc tcgacaagca
     2161 cgagctccgg gcgctcgtcg acgacctggc ggcccgcccc gagctgtgga gcgggcacgt
     2221 cgcgttctcc gacgagcggc gccactacgt ctccctgcac cgcgacgagc acgtggacgt
     2281 atggctgctc tgctggacca ggaccaacga caccgggtgg cacgaccacg acgtctcctc
     2341 gggggcggtc cacgtcgtgc ggggcgccat cgccgagtgc aacccgcggc tcggcggcga
     2401 gcacgtggtc accgtggtct ccgagggccg gtcgttctgc ttcgggcccg accacatcca
     2461 ccggatgagc ggcgagcgcg agggcggcgt gacgatccac gcctactcgc caccgctctg
     2521 gcggctcggc cagtacgcct tcggcaccga cggcgtcatg cgccgcgtct cggtcagcta
     2581 cgccgaggag ctgcggccgc tcgacccggc ggcctgacgc gcccggtccg cggcagcggg
     2641 gtggtccggc acgccccggc gcgggccccg aggcggaaca atgtccggca ggaacggcgc
     2701 cggtatcccc tgaccgttcc cggccctcga cgaggacgga ggaaggatgg ccatcaccct
     2761 gctgccgccc ggccgtgcga ccggcacctt cctcgacgcg ccgccgcacc acgacccttc
     2821 caccgtggca agcagcttcg cggtcgtcgg ggtgccctgc gcggcaccgc cgggctcgca
     2881 cgcggacggc ggagcgccgc cggacgcctg cgacgccccg gcggtgctcc gccgccgctc
     2941 gcagcggttc gccgggtcgc gctcgcacga cgacggcgac ggcgacctcc tcggcggccc
     3001 ggtgtcgctg gtggactgcg gcgacgtccc ttccgacccg cacgacccgt cccggcagga
     3061 ggagcacgcg acccgggcgg tccgtgcgct cctcggacgc ggcgtcgtgc cggtcgtgct
     3121 cggcggcgac cgctccgtcc cggccggcgt cctggcggcc taccagggct ccgggccgct
     3181 ccaggcaatc gcgatcgggg cgcacccgcg ctcccgcgag gaggccggcg gcctgctggt
     3241 cgacgcggcc ggcgtgcggg tgcacggcgt cgactggctg ctcggccggc ttccggagga
     3301 cgggcggttc ctcgtcaccc tggacgtgga cggcctcgac ccgtcgatcg cgccggggac
     3361 ggaccgccca cgcgagggcg gcctgctgtt ccacgaggcg gcggccctgc tgcgcggcct
     3421 cgcggcccgc ggcggggtcg ccggcgtctg cgtcgtcggc ctcttcccct ccctggacgt
     3481 cgaccagacg acggccgaca cggtggtccg gctgctcgcg agcctcatgc gggcggccgg
     3541 gcggaaggcc ggcacggctc ccgtgctgct cgacggcgag cgccttcgga tcgtcgacgt
     3601 ggagcgcatc gccggcggca cggccgcggt cgaggtgccg gaggcggcgt ggcagcggat
     3661 cgcccggggg cgggccgtgg tcgagccgct ggcggaccgg cgcgtgcggg cgtacggcgt
     3721 cacgaccggg gtgggctcgc acaaggacta catggtcgag ccggagcggc tcgaggagtt
     3781 caacctgcga ctgctctacg cgcacgccac ccgctcgccg gggccggtgg ccccggcgga
     3841 ggtcgtgcgc gcctgcatgg cgatcctggt gaacctcttc gccagcgggc gctccggggt
     3901 ccggccagcg ctggtcgagc ggttgctggc cgacctgcgc gaggaccgcc tgccagcggt
     3961 cgacctcggc agctcggtcg gcgcctccga cctcgtggcg ctggcgcagc tcgcgctgcc
     4021 cctggtcggc gggggcgagc tgctcgatgg cggcgagctc gggccggagg agcggcctcc
     4081 gggcgcgcct gtcttccccg ctccgaagga ggcgctggcg ctcgtcaaca acaacagcct
     4141 caccctcggc cgcggcgcgc tcgtcctggc cgaggcgcgc aggctcctcg tcgccatgga
     4201 catcgccgcg gcgctggcgc tggaagggtt ccggggcaac ccgcgggcat ggagcgccgt
     4261 ggtcgagcag gcccaccccc agcccggcca ccggtgggcg ggggagcggc tgcgccggct
     4321 gctcgcgggg agccgcctct ggcgacccgg cgagccgcgg ttcctgcagg acccgctcag
     4381 cttccgctgc gtccctcagg tgagcggcgc ggccagggag gcgctcgcct ggacgtggcg
     4441 gacctgggag cgggagctca acgccacggt cgacaacccc ctggtcgacc tcgcgagcgg
     4501 cgaggtcgtg acgcacggca gcatggagac gacgctgctc gccgtcacca tggacgcgct
     4561 caggctcacg ctcgccaagg cgctccggac gagcgcggag cgaacccaca agctgcactg
     4621 gtcgagcttc agcggccttc ccatggggct ggccgccgag gccggcgcct cgggcggggc
     4681 ccagttcctc aacctcagct acatcgtcgc cgcccacgtc gcgacctcgt cgatcgcggc
     4741 gacgcccgtc accccccact accacgggca gctcgcggaa ggcgtggaag acatcgccgg
     4801 tctcgcgccc cacgcggtga ccggggcgga gcggctgctc cagagcgcct gggtggtcgt
     4861 cgggatcgag gcgctggtgg cgtcctgggc ggtggcacgc cgcggcctgg ccccggccga
     4921 cctcggaacg gcgctgcgcc ccgcccacca gatgctcctg cccctcgttc ccgccgggcg
     4981 cgagggcgag cggcagctcg acgtcagccc ggccgtcgag gccctgcggt cgctcgcccg
     5041 aacctggacg gagtgacgcc gccatgccgc gtgctcagct cacgaccggg gcctgggtgt
     5101 gctgatggcg ctggacatcc tcgaaaccg
//
LOCUS       DASPYY010000364         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_763784, whole genome shotgun sequence.
ACCESSION   DASPYY010000364 DASPYY010000000
VERSION     DASPYY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763784"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VEP73_05620"
     CDS             <1..677
                     /locus_tag="VEP73_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25944.1"
                     /translation="AAARADPVLADRSLAAFAYALDGHPDAAVAALAMGAKVWFRLGG
                     VAVAWRPLPAAVPAREVPAAGAAGPDRVALLSLIGSGLHLAELLRVRLGNLGSLDADA
                     RILPDPEAEPLAVAFEQRRGRTAERVTFLSFQARRAVLDDLERRGRAGLDTGPDAPLV
                     AGPDGGPATRATVATARRRSSALIRAGNTLNVDLCRTTGEFFRSWGMPGSRFVPPAAD
                     DGGEDP"
     gene            674..1699
                     /locus_tag="VEP73_05625"
     CDS             674..1699
                     /locus_tag="VEP73_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYT25945.1"
                     /translation="MKAVRLHKYGERPTVEQVPEPRATGPLDVVVRIGGAGLCRTDLH
                     IYEGQWADRSGVELPYTLGHENAGWVAEVGPAVANVAPGDTVILHPLVTCGLCRACRA
                     GDDVHCESSSFPGIDADGGMADLLRTSARSVVKLDPSLQPADVAALADAGLTAYHAVK
                     KAAPLLYPGTTVAMIGAGGLGHIGIQSLKALTPAEVIVVDRSEAALELARSLGADHTV
                     VADGGQVDRVLELTGGKGAHVVIDFVGERGAESDAWAMTREAGSDFVVGYGGTVDVPT
                     IDIISTERNVIGNLVGSYNDLAELMTLTAQGKVKLHTQTYPLDAVNDAMDDLEGGRLQ
                     GRGILVP"
     gene            1742..>3277
                     /locus_tag="VEP73_05630"
     CDS             1742..>3277
                     /locus_tag="VEP73_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT25946.1"
                     /translation="MDSPPARRQDLVDVLHGVEVADPYRWLEDPSSDETARWGEAQDD
                     LARPYLDGLPGRDVLRGRLRELLSAGLVTVPVLRGDRAFFMRREAGQEHAVLVVREGD
                     VERVLIDPSALSDDDTTTLDGWVPSVEGDRLAYWLSEGGDEEASLRVMEVATGEVLDG
                     PIDRTRYCPLAWLPGGEAFYYGRRLPASAVAEGEDRFHRRVWRHRVGADPATDDLVFG
                     EGRDKTEYHSLDVSLDGRWLLVGASAGTAPRNDLYIADLAGDGTLRPIQQGVDAETGG
                     GVERDGRLWLRTNLGAPRFRIVVADPARPEPDAWRDVVAESDAVIEGFALTDDAVVVA
                     STRHAVGRVAVHARATGALRTEVELPGLGSVHGVSSRPEGGDDVWIGWTDFTTPPGVC
                     RYGVSSGALELWMDAPGEVEIKGVTASQDVYRSKDGTEVRMFVLHREGLEPDGRRPTI
                     LYGYGGFNVPLTPEYSAGINAWVEQGGVYAIANLRGGSEEGEAWHRAGMRERKQNVFD
                     DFAA"
BASE COUNT          412 a         1246 c         1216 g          403 t
ORIGIN      
        1 tggccgcggc gcgcgccgac cccgtcctcg ccgaccggtc gctggccgcg ttcgcgtacg
       61 cgctcgacgg ccaccccgac gcggccgtcg ccgccctcgc catgggcgcc aaggtgtggt
      121 tccgcctcgg cggggtcgcg gtcgcgtggc ggccgctgcc cgccgcggtc cccgcccggg
      181 aggtgccagc cgccggagcg gccgggcccg accgggtcgc gctgctctcc ctgatcggga
      241 gcgggctgca cctcgccgag ctgctccgcg tccgcctcgg gaacctgggg tccctggacg
      301 cggacgcgcg gatcctgccc gacccggagg ccgagccgct cgcggtcgcg ttcgagcagc
      361 gccgcggccg gaccgccgag cgcgtcacct tcctgtcctt ccaggcccgc cgcgcggtcc
      421 tcgacgacct ggagcgccgt ggccgcgccg ggctcgacac cgggccggac gcgccgctgg
      481 tggccggtcc cgacggcggt ccggcgacgc gggcgacggt cgcgaccgcg cggcgccgct
      541 cgagcgcgct catccgggcc gggaacacgc tgaacgtgga cctgtgccgg acgaccgggg
      601 agttcttccg ctcctggggt atgcccggct cgaggttcgt cccacccgcg gccgacgatg
      661 gaggcgagga cccatgaagg cggtacgcct gcacaagtac ggggagcggc cgacggtcga
      721 gcaggtgccc gagccccggg ccaccggccc cctcgacgtc gtcgtacgga tcggcggtgc
      781 cggcctgtgc cgcaccgacc tgcacatcta tgagggccag tgggcggacc ggtcgggtgt
      841 cgagctgccc tacaccctgg ggcacgagaa cgccggctgg gtcgccgagg tcggcccggc
      901 ggtcgccaac gtcgcccccg gcgacaccgt gatcctgcac ccgctggtca cctgcgggct
      961 gtgccgcgcc tgccgggccg gcgacgacgt gcactgcgag tcctccagct tccccggcat
     1021 cgacgccgac gggggcatgg cggacctgct gaggaccagc gcccgctcgg tcgtcaagct
     1081 ggacccgtcg ctgcagccgg ccgacgtggc cgcgctggcc gacgcggggc tgacggccta
     1141 ccacgcggtg aagaaggcag cgcccctcct ctacccgggg acgacggtcg ccatgatcgg
     1201 ggcgggcggg ctcggccaca tcggcatcca gtcgctcaag gccctcaccc cggccgaggt
     1261 catcgtggtc gaccgctccg aggccgcgct cgagctggcg cgctccctcg gcgccgacca
     1321 caccgtcgtg gccgacggcg gccaggtcga ccgcgtcctc gagctcaccg gcggcaaggg
     1381 cgcccacgtg gtcatcgact tcgtcggcga gcgaggcgcc gagtccgacg cgtgggcaat
     1441 gacccgcgag gccggctccg acttcgtcgt cggctacggc ggcacggtcg acgtccccac
     1501 catcgacatc atctcgaccg agcggaacgt catcggcaac ctcgtcggca gctacaacga
     1561 cctcgccgag ctgatgacgc tcaccgccca gggcaaggtg aagctgcaca cccagaccta
     1621 cccgctggac gccgtcaacg acgcgatgga cgacctcgag ggcggccgcc tgcagggccg
     1681 cggcatcctc gtgccctgac ccatggggtc ccggtcctcc gggccggggt acagtggctc
     1741 gatggactcc cccccggcgc gacgccagga cctcgtcgac gtgctccacg gcgtcgaggt
     1801 cgccgacccc taccgctggc tcgaggaccc gtcgtcggac gagaccgccc ggtggggcga
     1861 ggcgcaggac gacctcgccc gcccgtacct cgacgggctg cccggccggg acgtgctgcg
     1921 cggccggctg cgggagctgc tgtcggccgg gctcgtcacg gtcccggtcc tgcggggcga
     1981 ccgggcgttc ttcatgcgcc gggaggcagg gcaggagcac gccgtcctgg tcgtccgcga
     2041 gggcgacgtc gagcgggtcc tgatcgaccc gagcgcgctg agcgacgacg acaccaccac
     2101 cctggacggc tgggtcccct cggtcgaggg cgaccggctc gcctactggc tgagcgaggg
     2161 cggcgacgag gaggcgtccc tgcgggtcat ggaggtcgcc accggcgagg tgctcgacgg
     2221 ccccatcgac cgcacgcgct actgcccgct ggcctggctg cccggcggcg aggcgttcta
     2281 ctacggccgc cggctgcccg cctccgccgt ggccgagggc gaggaccgct tccaccgccg
     2341 ggtctggcgc caccgggtcg gcgccgaccc cgccaccgac gacctcgtgt tcggcgaggg
     2401 ccgcgacaag accgagtacc actcgctcga cgtgtccctg gacggccgct ggctgctcgt
     2461 gggcgcgagc gccggcacgg cgccgcgcaa cgacctctac atcgccgacc tcgccggcga
     2521 cgggacgctg cggccgatcc agcagggcgt cgacgcggag acgggcggcg gggtcgaacg
     2581 cgacggccgg ctctggctgc gcacgaacct gggcgcgccg cggttccgga tcgtggtcgc
     2641 cgacccggct cggcccgagc ctgacgcatg gcgcgacgtc gtcgccgagt ccgacgcggt
     2701 catcgagggc ttcgcgctga ccgacgacgc ggtcgtggtc gcgagcacca ggcacgcggt
     2761 cggccgggtc gcggtccacg cccgggccac cggcgcgctg cggaccgagg tggagctgcc
     2821 cgggctcggc tcggtgcacg gggtgtcgtc caggcccgag ggcggcgacg acgtctggat
     2881 cggctggact gacttcacga ccccgccggg ggtctgccgg tacggggtgt cgagcggcgc
     2941 gctcgagctg tggatggacg ccccgggcga ggtcgagatc aagggggtca cggccagcca
     3001 ggacgtctac cgctccaagg acggcaccga ggtccgcatg ttcgtcctgc accgcgaggg
     3061 cctggaaccc gacgggcgcc gcccgacgat cctctacggc tacggcggct tcaacgtgcc
     3121 cctcaccccc gagtacagcg ccggcatcaa cgcctgggtc gagcagggcg gcgtctacgc
     3181 gatcgccaac ctccgcggcg gcagcgagga aggcgaggcc tggcaccggg ccggcatgcg
     3241 ggagcgcaag cagaacgtct tcgacgactt cgccgcc
//
LOCUS       DASPYY010000365         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1890044, whole genome shotgun sequence.
ACCESSION   DASPYY010000365 DASPYY010000000
VERSION     DASPYY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890044"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1722)
                     /locus_tag="VEP73_05635"
     CDS             complement(<1..>1722)
                     /locus_tag="VEP73_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT25947.1"
                     /translation="RTLTRTLGVEWTDADRHGNRELKGVPEALVRAFSKRTDQIDQQI
                     GRLEADGRERTPRLVKWAVHSTRKAKTHEEPETLYGRWRQEAAERGVDADTLVRQVTG
                     RARDRTSGVSDQTVERLFDHLAGPDGLTASVSTFARPDVLVALGGGLAGATRTELEGL
                     ADRFCAERAVSMVADRALEQRRWSTPELLGVEGRLVAAATGRANDATGVVAHEAVRAA
                     LAASPTVESSGEQAGLVRDVCLDGAGVRVVVGRPGTGKTFTLGVARHAFQLDGYRVLA
                     AAPTGIATVSLEAEGFEEVATIDRLLLDLDHDQHRAGRHQASRPERALLDARTVLVVD
                     EAGMVGSRTLGRLLDHAEQAGAKVVLAGDDRQLAAIDAGGGFRALRVRLGASELVENR
                     RQLHAWQREALELVRQGLVEDAVAVYRAHDRVVVGDSKQAVTRALLTDWWRAWQDAER
                     DPARDVVVLAARRDEVDRLNSYCQQVMASNHRLGPDRLRVEDREVAVGDRIVCGKNAL
                     GRLGVANGTCGTVTALDPHARSLTIRLDGPDRREVTLPGWYLDGRQSSERNRRVDLAY
                     ATTGHRAQ"
BASE COUNT          186 a          661 c          640 g          235 t
ORIGIN      
        1 ctgcgcgcgg tgcccggtgg ttgcatacgc caggtcgacc cggcggttcc gctcgctgga
       61 ctggcggccg tccaggtacc agccgggcag ggtcacctcc cgcctgtccg gcccgtcgag
      121 gcggatggtc aggctcctgg cgtgggggtc gagggcggtg acggtgccgc aggtgccgtt
      181 ggcgaccccc agccggccga gcgcgttctt gccgcagacg atccggtcgc cgaccgccac
      241 ctcccggtcc tccacccgca gccggtcggg gccgaggcgg tggttgctgg ccatgacctg
      301 ctggcagtag ctgttgagcc ggtccacctc gtcccttcgg gcggcgagca ccaccacgtc
      361 gcgggcaggg tcgcgttcgg cgtcctgcca ggcgcgccac cagtcggtga ggagggcgcg
      421 ggtgacggcc tgcttggagt cgccgacgac cacccggtcg tgtgcccggt acacggcgac
      481 ggcgtcctcc accaggccct ggcggaccag ctcgagcgcc tcgcgctgcc aggcgtgcag
      541 ctgccgccgg ttctcgacca gctcgctggc gccgagccgg acgcggaggg cgcggaagcc
      601 gccgccggcg tcgatcgcgg cgagctgccg gtcgtcgccg gccagcacca ccttcgcgcc
      661 ggcctgctcg gcgtggtcga ggagccggcc gagggtgcgg gagccgacca tgccagcctc
      721 gtcgaccacc agcaccgtcc gggcgtccag cagcgcccgc tctggccggc tggcctggtg
      781 gcggccggcg cggtgctggt cgtggtcgag gtcgagcagg agccggtcga tggtggccac
      841 ctcctcgaac ccctcggcct cgaggctgac ggtggcgatc ccggtgggcg cagcggccag
      901 cacccggtag ccgtccagct ggaaggcgtg ccgggcgacc ccgagcgtga aggtcttgcc
      961 ggtgcccggc cggcccacca ccactcgcac cccggcgccg tccaggcaca cgtcgcggac
     1021 caggccggcc tgctccccgc tggactcaac cgtcggggag gccgcgaggg ccgcgcggac
     1081 cgcctcgtgg gccaccaccc cggtggcgtc gttggcgcgg ccggtcgcgg cggcgaccag
     1141 ccgtccctcg acgcccagca gctctggggt ggaccagcgg cgctgctcga gcgcccggtc
     1201 ggcgaccatg ctcacggccc gctccgcgca gaaccggtcg gccaggccct cgagctcggt
     1261 gcgggtcgcg ccggccagcc cgccgccgag cgcgaccagg acgtcggggc gggcgaacgt
     1321 ggacacgctc gcggtcagcc cgtccgggcc ggcgaggtgg tcgaacagcc gctcgaccgt
     1381 ctggtcggac acccccgatg tccggtcccg ggcccggccg gtcacctgcc ggacgagcgt
     1441 gtccgcgtcc acgccccgct cggcggcttc ctgacgccag cggccgtaca gcgtctcggg
     1501 ctcctcgtgg gtcttggcct tcctggtcga gtggacggcc cacttcacca gcctaggggt
     1561 ccgctcccgc ccgtccgcct caagccgtcc gatctgctgg tcgatctggt cggtccgctt
     1621 ggagaacgcc cgcaccaggg cctcaggcac gcccttgagc tcgcggttgc cgtgccggtc
     1681 ggcgtccgtc cattccaccc cgagggtccg ggtgagggtc cg
//
LOCUS       DASPYY010000366         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_625218, whole genome shotgun sequence.
ACCESSION   DASPYY010000366 DASPYY010000000
VERSION     DASPYY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625218"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VEP73_05640"
     CDS             <1..962
                     /locus_tag="VEP73_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25948.1"
                     /translation="RRGDSMSVVEQGTGVPAGRRARAGERLVTCEQLFHIYRLAGQEV
                     VSLQGVDLAVDRCEMVALLGPSGSGKSTLLSILGGLERPSAGRVLVEDADLAKLPEAA
                     LTAFRRRRVGFVWQVPARNLLPHASAAENLELPMTLAGLPAGRRRRRARVLLAAVDLD
                     GAGGKQVRALSGGEQQRLAVAVALANNPPLLLADEPTSQLDRPNVARVVEILHTARED
                     LGTTVVVVTHDDEVAARMDRTVTIRDGRVGAEGRAGTEYTVVGPTGSLHLPAHALDVL
                     PPGARAVVEVEGDRVVLRPVEPGAPATRRPGAEPGGDPWGVAR"
     gene            959..1699
                     /locus_tag="VEP73_05645"
     CDS             959..1699
                     /locus_tag="VEP73_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT25949.1"
                     /translation="MSRPGGGGAPVLAAEGVVARRGGRNVVDGVSVAAAAGRVTGVVG
                     PSGSGKSTLLTLLAGLEPPDAGRVSFEGRDVAGLGEAGRRVLRRDRVAVVFQGYGLLA
                     LLTARENVELPHRVARRDPVAAAAASERWLGLFGLAGRADHRTDELSGGEQQRVALAR
                     AFASGADVLLVDESTAELDEENRGRVVGLLRDAADRGACVVVATHDPDVIEVCDQVVR
                     LVDGRLATNVDLTGGTAAPPAAPGRPRP"
     gene            complement(1647..>2025)
                     /locus_tag="VEP73_05650"
     CDS             complement(1647..>2025)
                     /locus_tag="VEP73_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25950.1"
                     /translation="VLSDGRHPVFIVSVDDVRRTMVVDEVQFLTGAAAAKAAAEDGRE
                     SPPPNDYYIRNQNPRKRTVPVAEGARITVNGLTEEETGSATKSVPLELGELASYFPNP
                     EHPLFWVTVEAGQALRVEQQYLP"
BASE COUNT          215 a          750 c          803 g          257 t
ORIGIN      
        1 cgcggcgggg tgattcgatg agcgtcgtgg agcaggggac cggcgtgccg gccgggcgcc
       61 gcgcccgggc gggggagcgg ctggtcacct gcgagcagct cttccacatc taccgtctgg
      121 ccgggcagga ggtcgtctcc ctgcagggag tcgacctggc cgtcgaccgg tgcgagatgg
      181 tcgccctgct cggcccgtcg ggctcgggga agtccaccct gctgtcgatc ctcggcggcc
      241 tcgagcggcc gtccgccggc cgggtcctgg tcgaggacgc cgacctggcc aagctgcccg
      301 aggccgcgct gaccgcgttc aggcgccgcc gggtcggctt cgtctggcag gtcccggccc
      361 gcaacctgct gccgcacgcc tcggccgcgg agaacctgga gctcccgatg accctcgccg
      421 gcctgccggc cggcaggcgc cggcggcggg cgcgggtgct gctggccgcc gtcgacctgg
      481 acggggcggg cggcaagcag gtccgcgccc tctccggcgg cgagcagcag cgcctggccg
      541 tggccgtcgc cctcgcgaac aacccgcccc tgctgctcgc cgacgagccg accagccagc
      601 tggaccgccc gaacgtcgcc cgggtggtcg agatcctcca cacggcacgg gaggacctcg
      661 gtacgaccgt cgtggtcgtc acccacgacg acgaggtcgc ggcgaggatg gaccgcaccg
      721 tcacgatccg cgacgggcgg gtcggcgccg agggccgggc cgggacggag tacacggtgg
      781 tcgggccgac cggctccctc cacctgcccg cgcacgccct cgacgtgctc cctccgggcg
      841 cccgggccgt cgtcgaggtc gagggcgacc gggtggtgct gcggcccgtc gagccggggg
      901 cgccggccac ccggcgaccg ggcgcggagc cgggcggcga cccgtggggg gtggcgcgat
      961 gagccgcccg ggaggcggcg gggcacccgt gctggccgcg gagggggtgg tcgcccgccg
     1021 gggcggccgc aacgtggtcg acggcgtgtc ggttgcggca gccgccggcc gggtgaccgg
     1081 cgtggtcggc ccgtccgggt cgggcaagtc gaccctgctc accctgctcg ccggcctgga
     1141 gccgcccgac gccggtcggg tcagcttcga gggccgcgac gtcgccgggc tgggggaggc
     1201 cgggcggcgg gtcctgcgcc gcgaccgggt cgccgtcgtg ttccagggct acggcctgct
     1261 cgcgctgctc accgcccgcg agaacgtcga gctgccgcac cgggtcgcga ggcgcgaccc
     1321 ggtcgcggcg gcggccgcgt ccgagcgttg gctgggcctc ttcgggctgg ccggccgggc
     1381 cgaccaccgc accgacgagc tctccggagg ggagcagcag cgggtcgcgc tggccagggc
     1441 gttcgcctcc ggcgcggacg tgctgctcgt cgacgagtcg accgccgagc tcgacgagga
     1501 gaaccgcggc cgggtcgtcg ggctgctccg cgacgcggcc gaccggggcg cctgcgtcgt
     1561 cgtcgccacc cacgacccgg acgtgatcga ggtctgcgac caggtggtgc gcctcgtcga
     1621 cggccggctc gcgacgaacg tggacctcac gggaggtact gctgctccac ccgcagcgcc
     1681 tggccggcct cgaccgtgac ccagaacagc gggtgctccg ggttcgggaa gtaggaggcc
     1741 agctcgccga gctccagggg gacgctcttg gtggcgctgc cggtctcctc ctccgtgagg
     1801 ccgttgacgg tgatccgcgc cccctcggcg accgggaccg tgcgcttcct cgggttctgg
     1861 ttgcggatgt agtagtcgtt cggcggcggc gactcccgcc cgtcctcggc ggccgccttg
     1921 gccgcggcgg cgccggtgag gaactggacc tcgtccacca ccatcgtgcg ccgcacgtcg
     1981 tcgacggaca cgatgaagac cgggtggcgc ccgtcgctca gcacg
//
LOCUS       DASPYY010000367         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2591101, whole genome shotgun sequence.
ACCESSION   DASPYY010000367 DASPYY010000000
VERSION     DASPYY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591101"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /gene="carB"
                     /locus_tag="VEP73_05655"
     CDS             complement(<1..1107)
                     /gene="carB"
                     /locus_tag="VEP73_05655"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYT25951.1"
                     /translation="MPLRDDLRSILVIGSGPIVIGQAAEFDYSGTQACRALRRLGLRV
                     ILVNSNPATIMTDPEVADATYLEPLTPDSVAAVLDAERPDALLPTLGGQTALNLAVAL
                     HERGDLERFGVELIGATVEAVRRAESRVAFSEVCRAAGLDLPRSVEVGTVEQGREAAA
                     DLGLPLVLRPSFTLGGWGGGIARAQHELDELLAQGLAASPVRRVLVEESVLGWKEFEL
                     EVMRDRADNAVVVCSIENLDPMGVHTGDSITVAPAMTLSDREYQAMRDDALTVLRAVG
                     VETGGANVQFAVDPATGRRVVIEMNPRVSRSSALASKATGFPIAKVAAQLAVGFTLDE
                     IRNEVTGKTAAAFEPTLDYVVVKVPRFNFEKFPDA"
     gene            complement(1107..1496)
                     /locus_tag="VEP73_05660"
     CDS             complement(1107..1496)
                     /locus_tag="VEP73_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25952.1"
                     /translation="MTEQQRTFRALGGVDAGTMAPAGEAAEALRGEFDRALKLKVSLR
                     GEADAAVSPTGRHTTAHDEAKLRILVASCEGASRLAIKLGLLTPGQVRSLWSEAIAKG
                     LYDGWDAGQSAYNHPEATPPPTPEGAP"
     gene            complement(1496..2641)
                     /gene="carA"
                     /locus_tag="VEP73_05665"
     CDS             complement(1496..2641)
                     /gene="carA"
                     /locus_tag="VEP73_05665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYT25953.1"
                     /translation="MSGAPAPAPAPAPAPALLALEDGTAFSGQAFTSQPFTVAAEVVF
                     NTAMSGYQEVLTDPSYRRQMVCMAAPQIGNYGTTAADAESDRIQVAAFLVREAARRPS
                     SWRAQRSLAAELDAAGVPGVEGIDTRRLVRHLRTAGAMRGALSSETLDAEKLRAVALA
                     APDPNGLNLIAEVTRATPLELPPDPGSGERHRVVLYDYGAKANIIRRLRRLGARVLVV
                     PAKTPAAEVLAWEPDGVMLANGPGDPATLREEVGAVGELLGKVPVFGICLGHQILGQA
                     VGGSTVRLKFGHRGINQPVVEVATGRVAVTSHNHGFAVDAGSLPGAVTVTHRNANDGV
                     VEGLECTDVPAFSVQYHPEAAPGPHDAHPLFERFAALMDEHRGGGSR"
     gene            complement(2638..3942)
                     /locus_tag="VEP73_05670"
     CDS             complement(2638..3942)
                     /locus_tag="VEP73_05670"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYT25954.1"
                     /translation="MLEGGRLIDPASGHDATADVLVRDGLIAAVGADANAAGAGVEAE
                     RLDCDGLVVCPGFVDLHVHLREPGREDAETIESGSRAAALGGYTAVCPMPNTDPVADN
                     AGVVEMVAARGREIGLVDVFPVGAVTLGQRGVELAELGAMARSAAQVDCFSDDGHPIP
                     TARLLRLALEYARAFDAVIADHCEEASLTDGAQMHEGEVSAILGLQGWPAAAEEMIVA
                     RDLLLAELTGGRLHLCHLSTAGAVELLRAAKARGVRVTAEAAPHHFTLTDEAARSFDP
                     VFKVNPPLREKTDLEAIRAALADGTIDAIATDHAPHAREDKEVEWQAAPPGMLGLETA
                     LALTLTELVEPGWLTLPQAVERLSAGPARCRRLPGHGGPVSAGAPANLTVFDPAARWT
                     VEAARLASRARNTPYAGRAVGGRVLHTLLRGAFTVRDGRAVR"
     gene            complement(3951..>4563)
                     /locus_tag="VEP73_05675"
     CDS             complement(3951..>4563)
                     /locus_tag="VEP73_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25955.1"
                     /translation="FPSFGGLRVAIVGDVLHSRVARSNVLALTTMGAEVTLVAPPTLL
                     PARVDSWGVSVSYDLDAVLPKQDVVMLLRVQLERQKAGFFPSLREYARLYGLDAQRLA
                     TMADHAIVMHPGPMNRGVEIAAEVADSLERSVIADQVANGISVRMALLYLLLGGPAIE
                     IDPSGGPAPAGESARGPSGRGTVPPEPPGSAPPPAPALEGAAL"
BASE COUNT          575 a         1557 c         1841 g          590 t
ORIGIN      
        1 ggcgtcgggg aacttctcga agttgaagcg cggcaccttg accacgacgt agtcgagggt
       61 cggctcgaac gcggcggcgg tcttgccggt gacctcgttg cggatctcgt cgagagtgaa
      121 gccgacggcg agctgggcgg cgaccttggc gatcgggaac ccggtggcct tggaggccag
      181 cgccgaggag cgcgacaccc gcgggttcat ctcgatcacc acccgccgcc ccgtggccgg
      241 gtcgacggcg aactggacgt tggcgccgcc ggtctcgacc ccgaccgcgc gcagcacggt
      301 gagcgcgtcg tcgcgcatcg cctggtactc gcggtccgac agggtcatgg cgggcgcgac
      361 ggtaatcgag tcgccggtgt ggacgcccat cgggtcgagg ttctcgatgg agcagaccac
      421 caccgcattg tcggcccggt cgcgcatcac ctccagctcg aactccttcc agccgagcac
      481 cgactcctcc accagcacgc gccgcacggg gctggcggcc agtccctggg cgagcagctc
      541 gtcgagctcg tgctgcgcgc gggcgatccc gccgccccag ccgccgaggg tgaaggaggg
      601 ccgcagcacc agcggcaggc cgaggtcggc ggcggcctcg cggccctgct cgacggtgcc
      661 gacctcgacg ctgcggggca ggtcgagccc ggcggcgcgg cagacctcgc tgaaggcgac
      721 gcgggactcg gcgcggcgga ccgcctcgac ggtggcgccg atcagctcca ccccgaagcg
      781 ctccaggtcg ccgcgttcgt gcagggcgac ggcgaggttg agggcggtct ggccaccgag
      841 ggtgggcagc agcgcgtcag gccgctcggc gtcgaggacc gccgcgaccg agtcgggggt
      901 gagcggctcc aggtaggtgg cgtcggccac ctccgggtcg gtcatgatcg tggcggggtt
      961 ggagttgacc aggatcaccc gcagcccgag ccgccgcagc gcccggcatg cctgcgtgcc
     1021 ggagtagtcg aactcggcgg cctggccgat cacgatcggc ccggagccga tcaccaggat
     1081 cgagcggaga tcgtcgcgca acggcactag ggcgcgccct ccggggtcgg cggcggggtc
     1141 gcctccgggt ggttgtaggc ggactggccg gcgtcccagc cgtcgtagag gcctttggcg
     1201 atcgcctcgg accacagcga gcggacctgc ccgggggtga gcaggcccag cttgatcgcc
     1261 aggcggctgg cgccctcgca actggcgacc aggatgcgga gcttggcctc gtcatgggca
     1321 gtcgtgtggc ggccggtcgg cgacacggcg gcgtcggcct cgccccgcag gctcaccttg
     1381 agcttcaggg cccggtcgaa ctcgccgcgc agtgcctcgg cggcctcccc tgctggcgcc
     1441 atggtgcccg cgtcgacgcc gccaagggca cggaaggttc gttgctgctc agtcatcagc
     1501 gggagcctcc tccgcggtgc tcgtccatga gcgccgcgaa tcgctcgaac agggggtggg
     1561 cgtcgtgggg accaggggcg gcctccgggt ggtactggac cgagaaggcg gggacgtcgg
     1621 tgcattccag gccctcgacc acgccgtcgt tggcgttgcg gtgggtgacc gtgacggcgc
     1681 ctgggaggct gccggcgtcg accgcgaagc cgtggttgtg gctggtgacc gcgacacggc
     1741 cggtggcgac ctccaccact ggctggttga tgccgcggtg gccgaacttg agccgcaccg
     1801 tgctgccgcc cacggcctgg ccgaggatct ggtggccgag gcagatcccg aagaccggca
     1861 ccttgccgag cagctcgccg acggcgccga cctcctcccg cagggtggcg gggtcgccgg
     1921 ggccgttggc cagcatcacc ccgtcgggct cccaggcgag cacctcggcg gcgggggtct
     1981 tggccggcac gaccaggacc cgggcgccga ggcgccgcag ccgccggatg atgttggcct
     2041 tggcgccgta gtcgtagagg acgacccggt gccgctcgcc gctgccgggg tcgggcggca
     2101 gctcaagggg ggtcgcgcgg gtcacctcgg cgatcaggtt gaggccgttg gggtcggggg
     2161 cggccagggc gaccgcgcgg agcttctcgg cgtcgagggt ctcgctggag agggcgcccc
     2221 gcatcgcccc ggcggtgcgc aggtgccgga ccaggcggcg ggtgtcgatg ccctcgacgc
     2281 cgggcacgcc ggccgcgtcc agctcggcgg cgaggctgcg ctgtgcccgc cagctggagg
     2341 gccgccgggc agcctcgcgg accaggaagg cggcgacctg gatgcggtcg gactcggcgt
     2401 cggcggcggt ggtgccgtag ttgccgatct gcggggcggc catgcagacc atctggcggc
     2461 ggtaggaggg gtcggtgagg acttcctggt agccgctcat ggcggtgttg aagacgacct
     2521 cggcggcgac ggtgaacggc tgcgaggtga acgcctggcc gctgaaggcg gtgccgtcct
     2581 ccagcgccag cagcgcgggg gccggggccg gagccggggc tggggcggga gcgccgctca
     2641 ccggaccgcc ctcccgtccc gcacggtgaa cgcgccccgc aggagcgtgt ggaggacccg
     2701 cccgccgacc gcgcggccag cgtagggggt gttgcgggcg cggctggcga gccgggccgc
     2761 ctcgaccgtc cagcgcgcgg ccgggtcgaa cacggtgagg ttggcgggcg ccccggcgct
     2821 gaccgggccg ccgtggccgg gcaggcgccg gcagcgggcc gggccggccg agagccgctc
     2881 gaccgcctgc gggagcgtca gccagccggg ttcgaccagc tcggtgaggg tgagcgccag
     2941 cgcggtctcc aggccgagca tgccaggcgg ggcggcctgc cactcgacct ccttgtcctc
     3001 gcgggcgtgg ggggcgtggt cggtggcgat ggcgtcgatg gtgccgtcgg cgagggcggc
     3061 gcggatcgcc tccaggtcgg tcttctcccg caggggcggg ttgaccttga agaccgggtc
     3121 gaaggagcgg gccgcctcgt cggtgagggt gaagtggtgg ggtgccgcct cggcggtgac
     3181 ccgcaccccg cgggccttgg cggcgcggag cagctcgacc gccccggcgg tggacaggtg
     3241 gcacaggtgc agccgcccgc cggtcagctc ggcgaggagc aggtcgcggg cgacgatcat
     3301 ctcctcagcg gcggcgggcc agccctgcag gccgaggatg gcggagacct cgccctcgtg
     3361 catctgggcg ccgtcagtga gggacgcctc ctcgcagtgg tcggcgatga ccgcgtcgaa
     3421 ggcgcgggcg tactcgagcg cgaggcgcag cagccgcgcg gtcgggatcg ggtggccgtc
     3481 gtcggagaag cagtcgacct gggcggcgga gcgggccatc gcgcccagct cggccagctc
     3541 gacgccgcgc tggccgaggg tgaccgcgcc gaccgggaac acgtcgacca ggccgatctc
     3601 gcggcctctg gcggcgacca tctcgaccac gccggcgttg tcggcgaccg ggtcggtgtt
     3661 aggcatcggg cagacggcgg tgtagccgcc gagcgcggcg gcgcgggagc cgctctcgat
     3721 cgtctcggcg tcctcccggc cgggttcgcg caggtgcacg tggaggtcca cgaacccggg
     3781 gcaaaccacc aggccgtcgc agtccagccg ctcggcctcc accccggcac cggcggcgtt
     3841 ggcgtcagcg ccgacggcgg cgatcaggcc gtcgcggacc agcacgtcgg cggtggcgtc
     3901 gtggccgctg gccgggtcga tcagccggcc gccctccagc atgagagcgc tcacagggcg
     3961 gctccttcca gcgcgggggc gggtggtggg gcgctgccgg ggggttcggg gggcacggtc
     4021 ccgcgtccgg aaggaccacg agccgattcc cctgcgggcg cggggccccc cgatgggtca
     4081 atctcgatgg cggggccgcc gagcaggagg tagagcaggg ccatgcggac gctgatgccg
     4141 ttggcgacct ggtcggcgat gaccgagcgt tccagcgagt cggcgacctc ggcggcgatc
     4201 tcgacgccgc ggttcatcgg gcccgggtgc atcacgatgg cgtggtcggc catggtggcg
     4261 aggcgttgcg cgtccagccc gtagaggcgc gcgtactcgc gcagggaggg gaagaacccg
     4321 gccttctggc gctccagctg aacccgcagc agcatcacca cgtcctgttt gggcaggacc
     4381 gcgtccaggt cgtaggagac gctgacgccc cagctgtcga cccgggcggg cagcagcgtc
     4441 ggcggggcga ccagggtgac ctccgcgccc atggtggtga gggcgaggac gttggagcgg
     4501 gccacccggg agtgcagcac gtcgccgacg atcgcaaccc gcaggccgcc gaagctcggg
     4561 aag
//
LOCUS       DASPYY010000368         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1748600, whole genome shotgun sequence.
ACCESSION   DASPYY010000368 DASPYY010000000
VERSION     DASPYY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748600"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VEP73_05680"
     CDS             <1..610
                     /locus_tag="VEP73_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HYT25956.1"
                     /translation="GDRALAAYVLGCMSVVATYGDDGHTGLQLAHEAQSRGKGSVTAT
                     TLAWLCRVEALALATLGEAGACTAALDRAETAIAEASPGSDPPWIYHFNRSQLAGQKG
                     ACYVRLHRPRDARAALGEALTTLSPDFVRDRSLHLTHLASAYAQQGEIEEACRLAGDS
                     LTNAMRTGSARELQRIHEFRRELEPWNETSAVKGLDAQLLMV"
     gene            complement(777..1391)
                     /locus_tag="VEP73_05685"
     CDS             complement(777..1391)
                     /locus_tag="VEP73_05685"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HYT25957.1"
                     /translation="MKRLGTSNLLRAALDYAERGIPVFPCHHPANNWALGRWSSRECS
                     CQQLGCLHAGEHPLSQRWREEATTDPSRIERWWSRHPEANVGLATGVVFDVLDVAGTP
                     DAYRLPRAVAPEGPVARTGGGRWHYFVLPTGLGDAAMPGRAGSGPRSHVHWHGRGGYV
                     LGAPSRHPNGAATRWVRPLDAALPEVPALVRTSLDPTAARTRPA"
     gene            1768..2583
                     /locus_tag="VEP73_05690"
     CDS             1768..2583
                     /locus_tag="VEP73_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT25958.1"
                     /translation="MTGRVERWSERVTRVLAPNPSAMTLEGTNTYVVCEPASGTALVI
                     DPGPPDDGHALRVAGACEGRRIELVLLTHAHADHAEGARELAGRAGAPLAALAPGWST
                     PGAPALRPDRALAAAGTRLVPVPTPGHSADHCCFVLAAERAMFTGDHVLGRGTTVVDW
                     PEGDMGDYFRSLSVLLDYDLDRLYPGHGPLVPDAGAKVDEYLAHRRMRERQVLGALAA
                     GDRTPSEVVARVYAEVDPALHPAAERSVRAHLAKLVREGRARVEGGAFVVDLQ"
BASE COUNT          343 a         1055 c         1004 g          363 t
ORIGIN      
        1 gggcgaccgt gccctggccg cctacgtgct cgggtgcatg agcgtcgtcg cgacctacgg
       61 ggacgacggc cacaccgggc tgcagctcgc ccacgaggcg cagagcaggg gcaaggggtc
      121 ggtcacggcc acgacgctgg cctggctctg ccgggtcgag gcgctcgccc tcgccacgct
      181 cggagaggcc ggggcctgca cggccgcgct cgaccgcgcc gagaccgcga tcgccgaggc
      241 gtcgccgggc tccgatccgc cctggatcta ccacttcaac cgctcccagc tcgccgggca
      301 gaagggtgcc tgctacgtgc ggctgcaccg cccgcgcgac gcccgcgcgg cgctcggcga
      361 ggcgctgacg accctcagcc cggacttcgt gcgcgaccgg tcgctgcacc tgacccacct
      421 ggcgagcgcc tacgcgcagc agggggagat cgaggaggcg tgccgcctgg cgggggactc
      481 gctcacgaac gccatgcgca cgggctccgc gcgcgaatta cagcgcattc acgagttccg
      541 ccgcgagctc gagccatgga acgagacgtc ggccgtgaaa ggcctcgacg cgcaactcct
      601 gatggtatag cgctaggcaa ctcttttggt ttttcttgcg gattcctgct gacgcgtggc
      661 ggtcaattcc gcgcagacgg ctgtgctcaa ttgtgcctac ggcgggggcg aggagtgtcc
      721 cccgaaagga atagtcacct cgagccgcgc tcccgccgga gccggggagc ggtcggtcag
      781 gccgggcggg tgcgcgcggc cgtggggtcc agcgaggtcc gcaccagggc cggcacctcg
      841 ggaagggctg cgtcgagggg gcggacccag cgggtcgccg ccccgttcgg gtgccggctc
      901 ggcgccccga gcacgtaccc tccccgtccg tgccagtgga cgtggctgcg ggggccggac
      961 ccggcccggc ccggcatcgc ggcgtcgccg agcccggtcg gcaggacgaa gtagtgccag
     1021 cggccgccgc cggtgcgggc caccgggccc tctggggcga cggccctggg gagccggtac
     1081 gcgtcgggcg tcccggcgac gtcgaggacg tcgaacacca ccccggtggc gagcccgacg
     1141 ttggcctcgg ggtggcgcga ccaccagcgc tcgatccggc tggggtcggt ggtcgcctcc
     1201 tcgcgccatc gctgggacag ggggtgctcc ccggcatgga ggcagccgag ctgctggcag
     1261 gagcactcgc ggctgctcca gcggcccagc gcccagttgt tcgcgggatg gtggcacgga
     1321 aagaccggga tcccccgctc cgcgtagtcc agagcggctc tcagcaggtt gctggtgccg
     1381 agccgcttca tcccgatcac tgcgtcaccc ccgggaactc ggtcgcgcga agcctgcggc
     1441 tcgtgcgttc gtgagagcga atcacccctg aaccggaagg gaaagaccct ttacaggtcc
     1501 ccgacatgtc ccctgcggta accgcttact ccccgcaatc gggccaggga ccagctcgcc
     1561 ctggtggcca ccggcagtag catgcccgca tgggtcgcaa cgccgcgccg tggcgcagca
     1621 gggaccggtc gtgagggcgg gagggccgga cgcccatgcg gtgccggctc gtccggccgc
     1681 gaccgtcgtg gtgctgccgt ccggcgagcg cgtccccctc ccggcggcgc gggacaccgg
     1741 gccgcccacg gcgcgggacg ctcggccgtg accggccgtg tcgagcgctg gagcgagcgt
     1801 gtcacgaggg tcctcgcccc caacccgtcg gcgatgacgc tcgaagggac gaatacctac
     1861 gtcgtctgcg agcccgcctc gggcacggcg ctggtcatcg accccggccc gccggacgac
     1921 ggccacgcgc tgcgggtggc cggggcttgc gagggccgcc gcatcgagct cgtgctgctc
     1981 acccacgccc acgccgacca cgccgaaggg gcgcgtgagc tcgccgggcg ggccggcgcc
     2041 cccctcgccg ccctcgcccc gggctggtcc acgccgggcg ccccggcgct gcggcccgat
     2101 cgggcgctcg ccgccgccgg cacccgcctc gtgcccgtgc cgaccccagg ccactcggcc
     2161 gaccactgct gcttcgtgct cgcggccgag cgggcgatgt tcaccggcga ccacgtcctc
     2221 ggccggggca cgaccgtggt cgactggcca gagggggaca tgggcgacta ctttcgctcg
     2281 ctctcggtcc tgctcgacta cgacctggac cgcctctacc cggggcacgg gccgctcgtg
     2341 ccggacgctg gggccaaggt cgacgagtat ctggcccacc ggcgcatgcg cgagcgccag
     2401 gtcctcggcg cgctcgccgc cggcgaccgg accccgtccg aggtggtcgc ccgcgtctac
     2461 gcggaggtcg acccggcgct gcacccggcc gccgagcgct cggtccgcgc gcacctggcc
     2521 aagctcgtgc gcgagggccg tgcgcgggtc gagggcgggg ccttcgtcgt cgatttgcag
     2581 tagaccaacc ctcctcgttt aaggtagtcg ccctgcgctc gtgcccccgg acgagcgcac
     2641 gcgacccacc cccagcctgg gaggcgctcc ttgcccgtcc gcgcccgcac cctcgccctg
     2701 ctcgccgtgc tgctcaccgc ctcgtcgctc ctcgtgcctc ccgccgccgc cgacgaccgg
     2761 aaggc
//
LOCUS       DASPYY010000369         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1890748, whole genome shotgun sequence.
ACCESSION   DASPYY010000369 DASPYY010000000
VERSION     DASPYY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890748"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VEP73_05695"
     CDS             <1..1078
                     /locus_tag="VEP73_05695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25959.1"
                     /translation="LRVLEGEDGGRVGVLLARARRVYWGGRPVPAGQVSGLSVAVEHR
                     GRGAGRELLRAYLAEAHDRGAAICTLFPATIHLYRRAGFEYAGTWTLYEAAARNLPVG
                     WPDGYRTVPLLPSDDPAPLMERFAPTLAGRSGQIERDAATWRNGILRERGTGLPQAFL
                     VDGPDGPDGWAVLKVDEQVSARDVTAGVEVIDWGAVSLGGWRSLLALAAGFSSLDATV
                     IWKGPDVEPLALLLREQDLRQVRQFRFMARVLDVPDAFAARGYPDGARGQLTLEVADG
                     TCPWVAGTWSVEVEGGEGKAARVAGQAGAPRTDAAGLAALFAGYVDPVDLARLGVVTG
                     LTAEDVSFLQAVHAGPRPWSADFY"
     gene            1108..2343
                     /locus_tag="VEP73_05700"
     CDS             1108..2343
                     /locus_tag="VEP73_05700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25960.1"
                     /translation="MDATYRAITEDEHAAVADLILHLGFGGPTPGEEYLRIAPAGQFR
                     VLAAEHGEPLSALRVGRLAQWWLGRRVPSAQVLHLATPPQHRGQGWGTRLLAGLLAEL
                     REAGVPTVSLRPSNLPFYRAAGFEVAGAWSLYEARCEHLPGRLGGFRARQVPLDDLAE
                     PSAVYERVAPSRHGAFARDDLSWRWIATRGRERAAVCFVLDRPDGLADPAKGPPGGPA
                     GWVVVGFDAQRGAPVPATYLQVLDWGCEPGADTALFALLAGYGPMGGVVRWSGPDPDP
                     ALFALPDQHARLTSRFHWMLRVVDVQRAFEARPYPEGATGRIALEVDDPVCPWNTGTW
                     RLELAGGAARVERSPGGEGGEQSAALATVRGLAPLFTGFTDPADLVRAGLLALPRPAD
                     LEFLRAAFASPRPWTAEVY"
     gene            complement(2346..>3098)
                     /locus_tag="VEP73_05705"
     CDS             complement(2346..>3098)
                     /locus_tag="VEP73_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT25961.1"
                     /translation="RAAIEEVLAGRLALRDLQLPPGYRDDAVLLFDDPTGSQAGFAAL
                     LGHALAAGWADEAEALRPFWREALERQDADLARVGLEAFLRRFGEAPQILAQLRKLRS
                     NVRVELVPSCYIGARWANVYGPGHMVCTFQVRPPGWSPDDTITLELVRRRAEALAEPS
                     RLSLLRMVALGEATGGRALAARSGLTPATVSRHMAQLARAGLVTSRMHGREVRYEVEA
                     ASLRSFGPHLLQVLGQPLPADPAAETGRREED"
BASE COUNT          334 a         1198 c         1181 g          385 t
ORIGIN      
        1 gctccgcgtg ctcgagggcg aggacggcgg ccgcgtcggc gtgctgctcg cccgtgcccg
       61 gcgggtgtac tggggcggcc ggccggtccc ggccgggcag gtgtccggcc tgtcggtcgc
      121 ggtcgagcac cgcggcaggg gcgccggacg cgagctgctg cgcgcctacc tcgcggaggc
      181 ccacgaccgc ggcgcagcga tctgcacgct gttcccggcc accatccacc tgtaccggcg
      241 tgccggcttc gagtacgcgg ggacctggac gctctacgag gcggccgccc ggaacctccc
      301 cgtcggctgg ccggacggct atcggacggt gccgctcctg ccgtccgacg accccgcccc
      361 gctgatggag cgcttcgcac cgaccctggc cggccgcagt ggccagatcg agcgggacgc
      421 ggccacctgg cgcaacggca tcctccgcga gcgggggacc ggcctcccgc aggcgttcct
      481 ggtggacggg cccgacgggc ccgacggctg ggccgtgctc aaggtcgacg agcaggtctc
      541 ggcccgcgac gtgaccgccg gcgtggaggt gatcgactgg ggcgcggtct ccctcggcgg
      601 ctggcggtcg ctgctcgccc tggccgccgg cttctcgtcc ctggacgcca ccgtcatctg
      661 gaaggggccg gacgtcgagc cgctcgcgct gctgctgcgc gagcaggacc tgcggcaggt
      721 ccgccagttc cgcttcatgg cccgcgtcct cgacgtcccc gacgccttcg ccgcccgggg
      781 ctacccggac ggcgcccgcg gccagctcac gctcgaggtg gccgacggca cctgcccgtg
      841 ggtcgcaggg acgtggagcg tcgaggtcga ggggggcgag ggcaaggcgg ccagggtggc
      901 gggccaggcc ggcgcccccc ggaccgacgc cgccgggctg gccgccctct tcgccggcta
      961 cgtcgacccc gtcgacctgg cccggctcgg cgtcgtcacc ggactgacgg ccgaggacgt
     1021 gagcttcctg caggcggtcc acgcgggccc caggccctgg agcgccgact tctactgacg
     1081 acccttctac cgacggaccg gaggggcatg gacgcgacct accgcgccat caccgaggac
     1141 gagcacgcgg ccgttgccga cctcatcctg cacctcggct tcggcggtcc gacgcccggc
     1201 gaggagtacc tgcgcatcgc ccccgctggc cagttccggg tgctcgccgc cgagcacggc
     1261 gagccgctct ccgccctgcg cgtcggccgc ctcgcccagt ggtggctcgg ccggcgggtg
     1321 ccgtcggccc aggtcctcca cctggccacc ccgccccagc accgcggtca gggctggggg
     1381 acccggctgc tcgccggcct gctcgccgag ctgcgggagg ccggggtgcc gacggtcagc
     1441 ctgcgcccaa gcaacctccc cttctaccgg gccgccggct tcgaggtcgc cggcgcgtgg
     1501 agcctctacg aggcgcgctg cgagcacttg cccggccggc tcggcgggtt ccgggcccgc
     1561 caggtgccgc tcgacgacct ggccgagccg agcgcggtgt acgagcgggt cgccccgtcg
     1621 cggcacggtg ccttcgctcg tgacgacctg tcgtggcggt ggatcgccac gcggggacgc
     1681 gagcgggccg cggtctgctt cgtgctcgac cgcccggacg gcctcgcgga ccccgcgaag
     1741 ggtcccccgg gcgggccggc gggctgggtg gtcgtcggct tcgacgccca gcggggcgcc
     1801 ccggtgccgg ccacctatct ccaggtgctc gactggggct gcgagcccgg ggcggacacc
     1861 gccctcttcg cgcttctcgc cggctacggc ccgatgggcg gcgtggtgcg ctggagcggg
     1921 cccgaccccg accccgccct gttcgcgctt cccgaccagc atgcgcggct caccagccgc
     1981 ttccactgga tgctgcgggt cgtcgacgtc cagcgcgcgt tcgaggcccg gccctacccg
     2041 gagggggcca cgggccggat cgccctcgag gtcgacgacc cggtctgccc ctggaacacc
     2101 ggcacgtggc ggctcgagct ggccggcggg gccgcgcggg tggagcggtc gcctggcggc
     2161 gagggcgggg agcagagcgc cgcactggcg accgtccggg gcctcgcccc gctgttcacg
     2221 gggttcaccg acccggccga cctggtccgc gccggcctcc tggcgctgcc tcggcccgcc
     2281 gacctcgagt tcctgcgcgc cgcgttcgcg agcccccgcc cctggacagc cgaggtctac
     2341 tgacgtcagt cttcctcccg gcggccggtc tcggccgccg ggtcggcggg gagcggctgc
     2401 ccgagtacct ggagcaggtg cgggccgaag ctgcgcagcg aggccgcctc cacctcgtag
     2461 cgcacctcac ggccatgcat gcggctcgtg accaggcccg cccgcgcgag ctgggccatg
     2521 tggcgggaga cggtcgcggg ggtcaggccc gagcgggcgg ccagggcgcg gccgccagtc
     2581 gcctccccga gcgcgaccat gcgcagcagc gagaggcggc tcggctcggc gagcgcctcg
     2641 gcccgcctgc gcaccagctc gagggtgatg gtgtcgtcgg gcgaccaccc cggcggccgg
     2701 acctggaacg tgcacaccat gtggcccggc ccgtacacgt tggcccagcg cgcgccgatg
     2761 tagcaggagg ggaccagctc gacgcggacg ttcgagcgca gcttgcggag ctgggcgagg
     2821 atctggggag cctccccgaa gcgccggagg aacgcctcga ggccgaccct ggcgaggtcg
     2881 gcgtcctggc gctccagcgc ctcccgccag aacgggcgca gcgcctcggc ctcgtcggcc
     2941 cagccggcgg ccagggcatg gccgaggagc gccgcgaacc cggcctggga gcccgtgggg
     3001 tcgtcgaaga gcagcaccgc gtcgtcccgg tagccgggcg gcagctgcag gtcgcggagg
     3061 gcgagccggc cggcgagcac ctcctcgatg gccgccct
//
LOCUS       DASPYY010000370         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_62471, whole genome shotgun sequence.
ACCESSION   DASPYY010000370 DASPYY010000000
VERSION     DASPYY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62471"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VEP73_05710"
     CDS             <1..413
                     /locus_tag="VEP73_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25962.1"
                     /translation="ARREAVTAGAQAEVVRALRSAPGETDPPAADELGMRAGVRYRAL
                     YERGFLDDEDFRGRLRDRELRGEEVRFVERVPVGMWVVDRRKSVISLNQTGVRSGPGT
                     WIVLEHPGLSDLLTDAFENVWAGAVPAGEAGTRI"
     gene            514..1437
                     /locus_tag="VEP73_05715"
     CDS             514..1437
                     /locus_tag="VEP73_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25963.1"
                     /translation="MTAQPVDETDLIDRLVRYGLGQGEARCYVVMLAPRSFRVSEVAQ
                     QAGLPRSRAYELVRSLVHSGLCTEVAGATMARFRVVPPSEAIGRLEAFANEQARRRNA
                     ALSGILHALEGLPAGRRTGSRHEPVDLLRRRDQVLDSYEQAMRDAREEVLCLAAMPWE
                     PVHSPAMLERLDGGGVSFRTVHERAVCASAPHREYVGLYAARGELCRVVDRVRVHFTM
                     FDRHSTILNLFSPGSEGPGAQLESLLIRHAGLAQLLHEAFERLWEHGVPFGQALREAG
                     IPGPAGTWPDPHGTSPPRHAQDGRFPERGTG"
     gene            1604..>1778
                     /locus_tag="VEP73_05720"
     CDS             1604..>1778
                     /locus_tag="VEP73_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011668071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HYT25964.1"
                     /translation="MRVVAGRLGGRRLRAVPGTSTRPTSDRVREALFAILGDRVEGAR
                     VLDLFAGTGALAIE"
BASE COUNT          217 a          648 c          660 g          253 t
ORIGIN      
        1 tggcccgtcg cgaggcggtc acggccgggg cgcaggccga ggtggtgcgt gccctgcggt
       61 ccgccccggg cgagactgac ccgcccgccg ccgacgagct cggcatgcgg gcgggcgtcc
      121 gctaccgcgc cttgtacgaa cgtggcttcc tcgacgacga ggattttcgc gggcggctcc
      181 gcgaccggga gctgcggggc gaggaggtcc gtttcgtcga acgcgtcccg gtcgggatgt
      241 gggtcgtcga ccggcgcaag agcgtcatca gcctgaacca gacgggcgtg cggagcggtc
      301 cgggcacctg gatcgtgctc gagcaccctg gcctctcgga cctgttgacc gacgccttcg
      361 agaacgtgtg ggcgggcgcc gtgcccgccg gtgaggccgg cacaagaatt tgagccggga
      421 aattccgccg tccgatttgt gcgccaacgt cttgacgtac acagtgcgcc tgcctactgt
      481 tcgcgtgccg tgttgtcccc gggggggagt ccgatgaccg cccagccagt cgatgagacc
      541 gatctcatcg atcgtctcgt ccgctatggc ctcggccagg gcgaggcgcg ctgctacgtg
      601 gtcatgctgg ctcccaggtc gttccgggtc agcgaggtcg cccagcaggc cggcctgccg
      661 cgctcgcgcg cctacgagct ggtccgctcg ctcgtccact ccgggctgtg caccgaggtc
      721 gccggcgcca ccatggctcg cttccgcgtc gtcccgccca gcgaggccat cggtcgcctt
      781 gaggcgttcg ccaacgagca ggcgcggcgc cgcaacgcgg ccctgtccgg catcctgcac
      841 gcgctggagg ggctacccgc gggtcgtcgg acgggctcgc gccacgagcc ggtggacctg
      901 ctccgccgcc gggaccaggt cctcgacagc tacgagcagg cgatgcggga cgcccgcgag
      961 gaggtcctct gcctggccgc gatgccgtgg gagcccgtcc acagcccggc gatgctcgag
     1021 cgcctcgacg gcggcggcgt cagcttccgc acggtgcacg agcgtgccgt ctgcgcgagc
     1081 gccccgcacc gcgagtacgt cggcctgtat gcggcgcgcg gcgagctgtg ccgggtggtc
     1141 gaccgcgtcc gcgtccactt cacgatgttc gatcgccact cgacgatcct gaacctgttc
     1201 agccccggca gcgagggccc cggcgcccag ctcgagtccc tgctgatccg gcatgccggg
     1261 ctggcgcagc tcctgcacga ggcgttcgag cgcctctggg agcatggtgt gccgttcggc
     1321 caggcgctgc gcgaggccgg gatcccgggc cccgcgggca cctggccgga cccgcacggc
     1381 acctccccgc cacggcacgc ccaagacggc cggttcccag aacggggaac tggctgagcc
     1441 ggttggaagg cgggggaacg gccttggccc ccccgggctc gacccccgcc tccaaccatc
     1501 cgggctccgg ggctcgagca gcgaagcggt agccccggct cgacgaaggg gctccggggc
     1561 tcgagcagcg aagcggtagc cccggctcga cgaggagggc gccgtgcgtg tcgtggccgg
     1621 ccggctcgga ggccgccggc tgcgcgccgt gccggggacg tcgacccggc cgaccagcga
     1681 ccgggtccgc gaggcgctgt tcgcgattct cggcgaccgg gtcgagggcg cccgggtgct
     1741 cgacctcttc gccgggaccg gcgcgctcgc catcgagg
//
LOCUS       DASPYY010000371         1972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1891246, whole genome shotgun sequence.
ACCESSION   DASPYY010000371 DASPYY010000000
VERSION     DASPYY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891246"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>1972
                     /locus_tag="VEP73_05725"
     CDS             48..>1972
                     /locus_tag="VEP73_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYT25965.1"
                     /translation="MTAALEFLLLGPLEARRRERPLRLGSIKHRMLLAKLLLHPNQVV
                     STDELIDTVWGGEPPPTVRQSLQNHVAALRKTIEPQAAPTPRTLLTRDPGYLLEVDPD
                     QVDLHRFQRLVAHGRRALAEGDARAAADLLGQALSLWRGPVLADVAAATEADWPELVG
                     VDELRLAALEARVEADLALGRHHELVGELEMLTRQHPLREQLHGQLMLALYRSGRQAD
                     ALAAYRDARRTLVGELGIEPNVSLQRLEQAILAQDPALELLAPARAAADDPAEAEDAP
                     PAGQPQAEAPGQTERKLVTVLFADVDEPVGEAGERDPEDVSSMLERHLERVRAEVASF
                     GGAVEHTIGGATMAVFGVPHTHEDDPERAVRAALAIRDALAGGGQDRPGGEPRAAAPG
                     VELRIAVTTGEALVTAGAGAGRVTGDLVTTCARLQQAAPAGTVLVSEATERATKRTVS
                     YGPASLLALSGRARPVTVWSALEPRDRTGLDAAGPSPVPLVDRERELRTLLESFERAR
                     ATCSPQLVTLVGPPGIGKSRLVAEAGRALRGDHDLIDWRQGRSPSYGEGVAFWALGEI
                     VKTEAGILETDNRARVDRKLAQAVGHALADDPDAADWVTGHLRLLVGADGEQPLQAGR
                     QEEAFAAWRRFLDGLAAR"
BASE COUNT          245 a          796 c          732 g          199 t
ORIGIN      
        1 ccagaggcca cccaggcccg caccgccccc cggacggggg ctcgccggtg accgccgcgc
       61 tcgagttcct gctgctcggc cccctcgagg ctcgccgcag ggagcggccc ctgcggctcg
      121 gcagcatcaa gcaccgcatg ctcctggcga agctgctgct gcacccgaac caggtcgtgt
      181 ccaccgacga gctgatcgac acggtctggg gcggcgagcc gccgccgacc gtcaggcagt
      241 cgctgcagaa ccacgtcgcc gcgctgcgca agacgatcga gccgcaggcg gcgcccaccc
      301 cgcgcacgct cctcacccgc gaccccggct acctcctgga ggtcgacccc gaccaggtcg
      361 acctgcaccg cttccagcgg ctcgtcgccc acgggcgccg ggcgctcgcg gagggcgacg
      421 cgcgggcagc ggccgacctg ctcggccagg cgctgtcgct gtggcgcgga cccgtcctcg
      481 ccgacgtcgc cgccgccacc gaggccgact ggccggagct ggtcggcgtc gacgagctgc
      541 ggctggccgc gctcgaggcg cgcgtcgagg ccgacctcgc cctcggcagg caccacgagc
      601 tggtcggcga gctcgagatg ctcacccgcc agcaccccct ccgcgagcag ctccacgggc
      661 agctcatgct cgccctctac cgctcgggca ggcaggccga cgcgctggcc gcctaccggg
      721 acgccaggcg cacgctcgtc ggcgagctcg gcatcgagcc gaacgtcagc ctgcaacgcc
      781 tggagcaggc gatcctcgcc caggatcccg cgctcgagct gctcgccccc gcccgggcgg
      841 cggcggacga ccccgccgag gccgaggacg caccgcccgc cggccagcca caggccgagg
      901 cgcccggcca gaccgagcgc aagctcgtca ccgtgctgtt cgccgacgtc gacgagccgg
      961 tcggcgaggc gggcgagcgc gaccccgagg acgtgtcgag catgctcgag cgccacctcg
     1021 agcgcgtgcg cgccgaggtg gccagcttcg gcggcgcggt cgagcacacg atcgggggcg
     1081 ccacgatggc cgtgttcggc gtcccccaca cccacgagga cgaccccgag cgcgcggtgc
     1141 gcgccgccct cgccatccgc gacgcgctcg ccgggggcgg ccaggaccgc cccggcggcg
     1201 agcctcgcgc cgccgcccca ggcgtggagc tgcgcatcgc ggtgacgacc ggcgaggcgc
     1261 tcgtcaccgc gggcgcgggc gcgggccggg tcaccggtga cctcgtgacc acctgcgccc
     1321 ggctccagca ggccgcgccg gcgggcacgg tgctcgtctc ggaggcgacc gagcgtgcca
     1381 ccaagcggac ggtcagctac gggccggcca gcctgctcgc gctcagcggc cgggcgaggc
     1441 cggtgaccgt gtggagcgcg ctcgagcccc gcgaccggac cgggctggac gccgccgggc
     1501 cgagcccggt gccgctggtc gaccgcgagc gggagctccg gacgctgctc gagtcgttcg
     1561 agcgggcccg ggcgacctgc agcccgcagc tcgtgaccct ggtcgggccg ccggggatcg
     1621 gcaagagccg gctcgtggcc gaggccgggc gggcgctccg gggcgaccac gacctgatcg
     1681 actggcgcca gggccgctcg ccctcctacg gcgagggcgt ggcgttctgg gcgctcgggg
     1741 agatcgtcaa gaccgaggcc ggcatcctcg agaccgacaa ccgcgcgcgg gtcgaccgca
     1801 agctcgccca ggccgtcggc cacgccctcg ccgacgaccc ggacgccgcc gactgggtca
     1861 ccgggcacct gcgcctgctc gtcggggcgg acggcgagca gccgctgcag gccgggcgcc
     1921 aggaggaggc cttcgccgcc tggcggcgct tcctcgacgg cctggccgcc cg
//
LOCUS       DASPYY010000372         1665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_344477, whole genome shotgun sequence.
ACCESSION   DASPYY010000372 DASPYY010000000
VERSION     DASPYY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344477"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VEP73_05730"
     CDS             complement(<1..216)
                     /locus_tag="VEP73_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25966.1"
                     /translation="MAAPRTVHLVPHTHWDREWYEPFQRFRMRLVDLLDRVLDRAEAD
                     PGFAFTLDGQTATIEDYLEIRPEAEGRV"
     gene            353..1534
                     /locus_tag="VEP73_05735"
     CDS             353..1534
                     /locus_tag="VEP73_05735"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HYT25967.1"
                     /translation="MTDQGPSSPAVPALDAWGVRRRSRLFPGPFLVHADLHNHTTLSD
                     GSGDPARAFASMRAAGLDAAALTDHSRWASAFLGLVEAPGWTGIDRTAWAATARLADE
                     ADASGEFAALRGFEWSSAIFGHMNVWGTERFTDPLATLLVSMAPWWRWLRRGGRGGLI
                     GFNHPGTGRLRFGQFRHQPDLADRVVSLEMFNKTLDYLFQGTDQGLESPLNQCLNAGW
                     RVGILGVSDEHGTDWGFPEGKGRAGLYVHELTRAGVLEALAARRFYAARVKGLRLDAA
                     VNGVRMGGTVPHARGPARFEVDLDRGEAWWGRPLNVQVLRPGPLLPTLAATVDVKLPT
                     PEEPVVAFETTLDRAEGAWVVLRVTDPAEPPDRRAQDEWATLGRAIAYSSPFWLGPAA
                     G"
BASE COUNT          190 a          630 c          627 g          218 t
ORIGIN      
        1 cacgcgcccc tcggcctcgg gccggatctc gaggtagtcc tcgatcgtgg cggtctggcc
       61 gtcgagcgtg aaggcgaacc cgggatcggc ctcggcccgg tcgagcaccc ggtcgagcag
      121 gtcgaccagg cgcatccgga agcgctggaa cggctcgtac cactcccggt cccagtgggt
      181 gtgggggacg aggtgcacgg tcctcggggc cgccatcgcc atgctccctt cggggtgtcg
      241 gacgccccgg tctccgggcg gtcgcggcca ttgtgccgcc gggccggcgg cccgtcaccc
      301 ggccgggtca cgcggccgtg cccacgcctg ccgggcgcgg taccgttcgg gcatgacgga
      361 ccagggaccc tcctcccctg ccgtcccggc gctcgacgcc tggggcgtgc gcaggcggtc
      421 ccggctcttc cccggcccct tcctggtcca cgccgacctg cacaaccaca ccacgctctc
      481 ggacgggtcc ggcgaccccg cgcgcgcctt cgcctcgatg cgggcggccg ggctggacgc
      541 cgccgccctc accgaccact cccgctgggc gtcggcgttc ctcgggctgg tcgaagcgcc
      601 gggctggacc ggcatcgacc gcacggcatg ggcggcgacc gcgcggctcg ccgacgaggc
      661 cgacgcgtca ggggagttcg ccgccctgcg cggcttcgag tggtcgagcg cgatcttcgg
      721 gcacatgaac gtgtggggca ccgagcggtt caccgacccg ctcgccacgc tgctcgtctc
      781 catggcgccg tggtggcgct ggctgcggcg cggcgggcgc ggcgggctga tcgggttcaa
      841 ccatcccggg acggggcggc tgcgcttcgg ccagttccgc caccagcccg acctcgccga
      901 ccgcgtggtc agcctcgaga tgttcaacaa gaccttggac tacctgttcc agggcaccga
      961 ccagggtctc gagtcgccgc tcaaccagtg cctgaacgcc ggctggcggg tcggcatcct
     1021 cggggtcagc gacgagcacg gcaccgactg ggggttcccc gagggcaagg gcagggccgg
     1081 cctgtacgtc cacgagctga ccagggcggg ggtgctcgag gcgctcgccg cccgccgctt
     1141 ctacgcggcc cgggtcaagg gcctgcggct ggacgcggcc gtgaacggcg tgcggatggg
     1201 cggcacggtg ccgcacgccc gaggccccgc ccggttcgag gtcgacctcg acaggggcga
     1261 ggcgtggtgg ggccggccgc tgaacgtgca ggtgctgcgg ccggggcccc tgctgccgac
     1321 cctggccgcg accgtcgacg tgaagctgcc gacccctgag gagcccgtgg tcgccttcga
     1381 gaccacgctc gaccgggccg agggcgcgtg ggtggtgctg cgcgtcaccg acccggccga
     1441 gccgcccgac cgccgggccc aggacgagtg ggcgacgctg ggccgggcga tcgcctactc
     1501 gagcccgttc tggctgggcc ccgccgccgg ctgacggcgg ggctcccgcc aggacgggcc
     1561 cgtcgcgggt ggtgccgtcg cgcggtcagg ccaccacgag cggcgcgcgc agcgcccgcc
     1621 ggaagatcgc ctcgtagccg ccgaccatcg tcgccacgtc gaact
//
LOCUS       DASPYY010000373         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1891635, whole genome shotgun sequence.
ACCESSION   DASPYY010000373 DASPYY010000000
VERSION     DASPYY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891635"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..640
                     /locus_tag="VEP73_05740"
     CDS             224..640
                     /locus_tag="VEP73_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYT25968.1"
                     /translation="MFKTILLAVDASKGAEKAAELASKLAVADGDEVVVVHVTEIMPA
                     RGGGADELDMDGESIDTARHYAKELEASGVPARADIRRALAGLAARVLVEAAEEHGAG
                     LIVMGSRGMTDLASLVLGSVTHKVLHLSTVPVLIAR"
     gene            822..1472
                     /locus_tag="VEP73_05745"
     CDS             822..1472
                     /locus_tag="VEP73_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HYT25969.1"
                     /translation="MRLSALERTKDRPSPAIARLLFHSWIGAILFLPVRLYFGWEWIK
                     GGWDKIDAGWLSSGKPVAGFAAGALKQTGGDHPAVAYGWWADFLRFQRDTAWFAHAMA
                     WTVAVGEVVIGVALILGFFTGIAAALGATLNFSFVFSGSAGVNPAYLLAEVLVIAAWR
                     VAGQLGADYWALNRVRALHLTGRQAAPTGKPTGKPTGKPTGKPTGKPTGKPTPTPV"
     gene            1691..2059
                     /locus_tag="VEP73_05750"
                     /pseudo
     CDS             1691..2059
                     /locus_tag="VEP73_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1974..2393
                     /locus_tag="VEP73_05755"
     CDS             1974..2393
                     /locus_tag="VEP73_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYT25970.1"
                     /translation="MRTIIVGVDGSDASIDAARHAGRLAADLDAALVVVHARQLPVVS
                     GPSAGDTGTVESIWKSVEEAALLRVTEALAPFGVRWKFEVGVGEPATLLERAAQQHEA
                     EMIVVGSRGHTATQRLLLGSVSTRLVHHADRPVLVVR"
     gene            2455..>4135
                     /locus_tag="VEP73_05760"
     CDS             2455..>4135
                     /locus_tag="VEP73_05760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT25971.1"
                     /translation="MSAVSAYPSHREADVVLRDGSTVHVRPVRQEDAAAVRSMLESMS
                     DASRWLRFFSAFPRLEPAVRWATEVDYDRRCGLVATAGGQVVAHAGYERDAGRADRAE
                     VALAITDAWQGKGLGTILLGQLAEVADQTGIVTFTAEVLPENHRMAQVFRDSGFPVTL
                     HALPGVVLVEFPTALTPGGLDRFERREQVAAVAAMRALLAPRSVAVVGASRTRGTVGG
                     ELFHNLLAAGFEGPVYPVNPTATVVQSVVAYPSVLDVPGPVDLAVVAVPADRVVETAR
                     QCAAKGVRGLVVISAGFAETGPEGAGRQAELLRLCREAGMRMIGPNCLGIVNTAPDVR
                     LDATFGPVVPERGRVGFLSQSGALGLAIVDYANALGLGLSSFVSVGNKADISGNDLLA
                     YWEQDDATDVILLYLESFGNPRKFARLARQVGRSKPIVAVKSGRSAAGARATSSHTGA
                     LLAASDATVDALFRQAGVVRTDTLAELFDVAALLANQPLPAGRRVAIVTNAGGPGILC
                     ADACEAGGLEVVPLSYGLRAQLAAFLPAAAATGNPVDMLASAPAEHHRRTLE"
BASE COUNT          542 a         1432 c         1591 g          570 t
ORIGIN      
        1 ccccgcccgg gccgcggcgg cgcggtggcc gaacggcccc gccgggggcg ggcgaacggc
       61 cctgtccgcg gtggagtgca tcgccgagaa ccgcatcctg cgccgcatcg caccgggctc
      121 gttcgaggag gacggggggg ctgttcgcct tcggccctcg gtccggattg gcttgcggcc
      181 gatcctgcaa ggcaggcgac atcgactgga gaggagatct ccgatgttca agaccatcct
      241 gctggcggtg gacgcgtcga agggcgccga gaaggccgcg gagctggcat caaagctcgc
      301 ggtcgccgac ggcgacgagg tcgtcgtcgt gcacgtcacc gagatcatgc cggctcgcgg
      361 cggcggcgcc gacgagctcg acatggacgg cgagtcgatc gacacggcga ggcactacgc
      421 caaggagctg gaggcgagcg gcgtgccggc gcgcgccgac atccgccgcg cgctcgccgg
      481 gctcgcggcc cgggtcctgg tcgaggcggc cgaggagcac ggtgccgggc tgatcgtgat
      541 gggttcccgc ggcatgacgg acctcgcgtc cctggtgctc gggagcgtga cgcacaaggt
      601 gctccacctg agcacggtgc cggtgctcat cgcacgctga cggcgcggtg gcaggtccga
      661 gtggtaggtc cgagtggagc cgaacggcct gcacgatccc gggccgggtc ccggggcttc
      721 cgggtcgggt ggccggtgcc gaggagcgca catgcggcga tgctgacggt gtcacaacga
      781 ccagattggc gtagcagaaa gaggaaagga ggggcgctgc catgagactg tcagcgctcg
      841 agcgcaccaa ggacaggccg agcccagcga tcgccaggct gctgttccac agctggatcg
      901 gcgcgatcct gttcctgccg gtgcggctgt acttcggctg ggagtggatc aagggaggtt
      961 gggacaagat cgacgccggc tggctgtcgt ctggcaagcc ggtggcgggg ttcgcggccg
     1021 gggcgctgaa gcagaccggc ggggatcacc ccgcggtggc gtacggctgg tgggcggact
     1081 tcctgcggtt ccagcgggac accgcatggt tcgcgcacgc gatggcgtgg acggtcgcgg
     1141 tcggggaggt cgtcatcggc gtggcgctga tcctcgggtt cttcacgggg atcgcggcgg
     1201 cgctgggagc gacgctgaac ttctcgttcg tgttctccgg gtcggctgga gtgaacccgg
     1261 cctacctgct ggccgaggtg ctcgtcatcg cggcatggcg ggtcgccggc cagctgggcg
     1321 cggactactg ggcgctgaac cgggtgcgcg cgctgcacct gacggggagg caggcggcac
     1381 cgacgggcaa gccgacgggc aagccgacgg gcaagccgac gggcaagccg acgggcaagc
     1441 cgacgggcaa gccgaccccg acaccggtgt gacggcacgc aggtacgacc agggggccct
     1501 ccggggcccc ctggtcgtac tcgtcataca cggacgagct cgcgccggcg agcaggacct
     1561 tgcgtccccc tcgacgcggg ccaccccgta tctggtctgg gccggggcgc atccatacgg
     1621 tggacatggc cgccaagggg acctgctccg ctcgaggaca ccgcccgggt cgtgagctgc
     1681 tacgccatcc gggctggagc cccatcccga ggtcgtcgcg cgggcgcgag ggctggccgt
     1741 cgagcagggc gcacgagtgc ggctcaccaa tgagcccgcg gtcgccgtgt caggcgccga
     1801 cgcggtcatc accgacgcgt ggatgtccat gcacgaccca ggggagaagg acccggcacg
     1861 gcggggacct tcgccctgtg ggcgtcaagg tgtctggtcc cggcgtcggc aggccgttcg
     1921 gccctcaccc atggggcccg tgccacgcag gctggacgag aggaggtgcc acgatgagaa
     1981 ccatcatcgt cggcgtcgac gggtcggacg cgagcatcga cgccgcccgg cacgccgggc
     2041 ggctggccgc ggaccttgat gcggccctgg tcgtcgtcca cgcacgccag cttcccgtgg
     2101 tctcggggcc ctctgcgggg gacaccggca cggtggagtc gatctggaag tcggtcgagg
     2161 aggcagcgct gctgcgcgtg accgaggcgc tcgcgccctt cggggtgcgg tggaagttcg
     2221 aggtcggcgt cggcgaacca gcgacgctgc tcgagcgcgc ggcacagcag cacgaggcgg
     2281 agatgatcgt ggtcggcagc cgcgggcaca cggcgacgca gcgtctgctg ctcgggtcgg
     2341 tgtcgacaag gctggtgcac cacgccgacc ggccggtcct ggtcgtacgc tgagcacgag
     2401 gtgagcatgc cgccgccgtc ggccgagccg aggcccggcg agagaggggg agcgatgtcc
     2461 gcggtctcgg cgtacccgtc ccaccgcgag gccgacgtcg tcctgcggga cggctcgacc
     2521 gtgcacgtcc gaccggtccg tcaggaggac gcggccgcgg tccggtcgat gctcgagagc
     2581 atgtccgacg cctcgcgctg gctgcggttc ttctccgcgt tccccagact ggagcctgcc
     2641 gtccgctggg cgaccgaggt cgactacgac cgccgctgcg gcctggtcgc gaccgcgggc
     2701 gggcaggtgg tcgcccacgc cggctacgag cgggacgccg gccgcgccga ccgggccgag
     2761 gtcgcgctcg cgatcaccga cgcctggcag ggcaaggggc tcggcacgat cctgctcggc
     2821 cagctcgccg aggttgccga ccagaccggg atcgtcacct tcaccgccga ggtcctgccc
     2881 gagaaccacc ggatggccca ggtcttcagg gactcgggct tcccggtgac gctgcatgcg
     2941 ctcccggggg tcgtcctggt ggagttcccg accgcgttga ccccgggggg gctggaccgg
     3001 ttcgagcgtc gcgagcaggt cgcggccgtc gccgcgatgc gggcgctgct cgcgccccgg
     3061 tcggtcgcgg tcgtcggcgc atcccggacc aggggcacgg tcggcgggga gctgttccac
     3121 aacctgctcg cggccgggtt cgaggggcct gtctacccgg tcaacccgac cgcgacggtg
     3181 gtccagtccg tggtcgccta tcccagcgtg ctcgacgtac ccggtccggt cgacctggcc
     3241 gtggtcgcgg tccccgccga ccgggtggtc gagaccgccc ggcagtgcgc cgccaagggc
     3301 gtgcgcggcc tggtcgtcat ctcggccggg ttcgccgaga ccggccccga gggcgccggg
     3361 cgccaggccg agctgctgcg cctctgccgg gaggctggca tgcgcatgat cggcccgaac
     3421 tgcctgggga tcgtcaacac cgcgcccgac gtgcgcctcg acgccacgtt cgggccggtc
     3481 gtgcccgagc gcggccgggt ggggttcctg tcgcagtccg gcgcgctggg gctcgcgatc
     3541 gtcgactacg ccaacgcact cggcctcggc ctgtcgtcgt tcgtgtcggt cggcaacaag
     3601 gccgacatct ccggcaacga cctgctcgcc tactgggagc aggacgacgc gaccgacgtg
     3661 atcctgctgt acctcgagtc gttcgggaac ccgcgcaagt tcgcccgcct ggcccggcag
     3721 gtcgggcggt ccaagccgat cgtggcggtc aagagcggca ggtccgccgc gggcgcgcgg
     3781 gcgacctcct cgcacaccgg ggcgctcctg gccgcgtccg acgcgaccgt cgacgcgctg
     3841 ttccgccagg ccggcgtcgt ccgcaccgac accctggccg agctgttcga cgtggccgcg
     3901 ctgcttgcca accagccgct gccggcaggg cggcgggtgg cgatcgtcac caacgccggc
     3961 gggccgggga tcctctgcgc tgacgcctgc gaggccggcg ggctcgaggt ggtcccgctc
     4021 tcctacgggc tgcgggcgca gctggcggcg ttcctgccgg ccgcggccgc caccggcaac
     4081 ccggtcgaca tgctcgcctc ggccccggcc gagcaccacc ggcgcacgct cgagc
//
LOCUS       DASPYY010000374         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_765595, whole genome shotgun sequence.
ACCESSION   DASPYY010000374 DASPYY010000000
VERSION     DASPYY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765595"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VEP73_05765"
     CDS             complement(<1..306)
                     /locus_tag="VEP73_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYT25972.1"
                     /translation="MSTLATAAGLDGPTGAALAELLGFMADDELVLGYWDSEWTGIAP
                     VLEEDVAFSSLAQDEIGHARALYELLAELTGGDADRIAYGREVGEYRQAALLDHARGD
                     "
     gene            complement(303..596)
                     /locus_tag="VEP73_05770"
     CDS             complement(303..596)
                     /locus_tag="VEP73_05770"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HYT25973.1"
                     /translation="MQVFEVFRQEREGGPMVHGGNLLAPDPELAAHYARELYGRRQES
                     VRLWVVPRSAVTDVTDPDLLQPPMDRSFKKPVGYSAGIKQKLAAARERVEGEA"
     gene            complement(647..1564)
                     /gene="paaA"
                     /locus_tag="VEP73_05775"
     CDS             complement(647..1564)
                     /gene="paaA"
                     /locus_tag="VEP73_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HYT25974.1"
                     /translation="MNDSVRLHAFNRHVAAGGKVEPGDWMPDDYRRAVGKFVEMHANS
                     ELMGVLPEREWILRAPTLKRKLALTAKVQDEVGHAQLLYRVAEDLGKPREAMIADLLA
                     GRTKFHNVFHYPTESWADVGIIAWLVDAAAIVAQQALRESSYAPYGRTMKKICWEESV
                     HILHGRDVVLTMVNGTARQRALVQDALDRWWGPLMQMHGPPTDPGKDRDLAWRIKNKD
                     NETLRQEFLTVYVPRVREIGLRIPDPGLRQDPDTGAWRYTEPDWRELYAVVTGHGPRS
                     AERLRFRQLNYEETRWVRDTVLAAPRAVA"
     gene            complement(1561..2034)
                     /locus_tag="VEP73_05780"
     CDS             complement(1561..2034)
                     /locus_tag="VEP73_05780"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25975.1"
                     /translation="MLWSIVEQAAQRFHEEVGPVASGPGSAAERLRAMVHAHVGVLVS
                     GLERASVYLFEWTFLAPERRELSARSRAAYQGLFEHVIAEGQAAGELGAADAKLAAMF
                     VLSAMNGLPRWYRPDGPLPPAAVAEQFADLFLHGLAPPASGVRAAPAHEREGAPR"
     gene            complement(2038..2181)
                     /locus_tag="VEP73_05785"
     CDS             complement(2038..2181)
                     /locus_tag="VEP73_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT25976.1"
                     /translation="MRPRRRQIEEVASSLFSERGYAATSMRDIARALDLQGGSLYAHI
                     PSK"
     gene            complement(2287..2712)
                     /locus_tag="VEP73_05790"
     CDS             complement(2287..2712)
                     /locus_tag="VEP73_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT25977.1"
                     /translation="MEAGWGPPGQRFRGVRKRFAGSADQVGAARRFVGGVLSRGSCAS
                     RAEETARLLVTEAATNALLHSASGDEGGSFEVRCQVDDDRRLRVEVHDAGAPTVPRRR
                     VHQLDAVTGRGLDLFEALASRWGLSGGSRGRVVWFELDL"
     gene            complement(2862..>3173)
                     /locus_tag="VEP73_05795"
     CDS             complement(2862..>3173)
                     /locus_tag="VEP73_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HYT25978.1"
                     /translation="GSGLHRLTTATGYEYAPAWSPDGRRLAYVKDGAIHVVDADGTGD
                     RQLSNGRRDAAPSWSPDGGRLAFVRDGSIFVARADGTGAACLPTGKPVVSRPAWQPTP
                     R"
BASE COUNT          410 a         1151 c         1170 g          442 t
ORIGIN      
        1 gtcgccgcgc gcgtggtcga gcaacgcggc ctggcggtac tccccgacct cgcggccgta
       61 ggcgatccgg tcggcgtcgc cgccggtcag ctccgcgagc agctcgtaga gcgcccgggc
      121 gtggccgatc tcgtcctggg cgagggagct gaacgccacg tcctcctcca gcacgggcgc
      181 gatgccggtc cactcggagt cccagtagcc gagcacgagc tcgtcgtcgg ccatgaaccc
      241 gagcagctcg gccagggcgg cgccggtggg cccgtccagc ccggctgcgg tcgcgagcgt
      301 gctcatgcct cgccctccac ccgctcgcgc gcggcggcga gcttctgctt gatgccggcc
      361 gagtagccga ccggcttctt gaacgaccgg tccatcggcg gctgcagcag gtccgggtcg
      421 gtgacgtcgg tcacggcgga ccggggcacc acccagagcc ggaccgactc ctggcgccgt
      481 ccgtagagct cgcgagcgta gtgggcggcc agctccgggt cgggggcgag caggttgcca
      541 ccgtgcacca tcgggccgcc ctccctctcc tgccggaaca cctcgaagac ctgcatctcc
      601 cgggggctgc cgccttctgc cgcatgagcc cccggacccc cggcggtcat gcgaccgccc
      661 ggggggcggc cagcaccgtg tcgcgcaccc accgggtctc ctcgtagttg agctgccgga
      721 acctcaggcg ctcggctgac ctggggccgt ggccggtgac gaccgcgtac agctccctcc
      781 agtcgggctc ggtgtagcgc caggcgccgg tgtctgggtc ctgccgcagg ccggggtcgg
      841 ggatccgcag gccgatctcg cggacccggg gcacgtacac ggtcaggaac tcctggcgga
      901 gggtctcgtt gtccttgttc ttgatgcgcc aggccaggtc gcggtccttc cccgggtcgg
      961 tcggcgggcc gtgcatctgc atgagcggcc cccaccaccg gtcgagggcg tcctggacga
     1021 gcgcccgctg cctggcggtg ccgttcacca tggtcagcac gacgtcgcgc ccgtgcagga
     1081 tgtggaccga ctcctcccag cagatcttct tcatcgtgcg cccgtagggt gcgtaggacg
     1141 actcgcgcag ggcctgctgg gcgacgatcg cggccgcgtc gaccagccag gcgatgatgc
     1201 cgacgtcggc ccagctctcg gtggggtagt ggaagacgtt gtggaacttc gtcctgcccg
     1261 cgagcaggtc ggcgatcatc gcctcgcgcg gcttgccgag gtcctcggcc acccggtaga
     1321 ggagctgcgc gtggccgacc tcgtcctgca ccttggcggt gagggccagc ttccgcttca
     1381 gggtcggcgc gcgcaggatc cactcgcgct cgggcagcac gcccatcagc tccgagttgg
     1441 cgtgcatctc gacgaacttg ccgaccgccc ttcggtagtc gtcgggcatc cagtcacccg
     1501 gctccacctt gccgcccgcg gcgacgtgac ggttgaaggc gtgcagccgc acggagtcgt
     1561 tcatcgtggc gctccttcgc gctcatgggc cggggcggca cggacgcccg aggcgggcgg
     1621 ggccagcccg tggaggaaca ggtcggcgaa ctgctcggcc accgcggcgg ggggcagcgg
     1681 cccgtcaggc cggtaccagc gcggcaggcc gttcatggcg gacaggacga acatggcggc
     1741 gagcttcgcg tcggcggccc cgagctcccc ggccgcctgg ccctcggcga tcacgtgctc
     1801 gaacagtccc tggtaggcgg cccgggaccg ggccgacagc tcgcgccgct ccggcgccag
     1861 gaaggtccac tcgaacaggt acacgctggc gcgctcgagc cccgagacga gcacgccgac
     1921 gtgggcgtgg accatcgccc ggagccgctc ggcggcggat ccgggaccgg aggcgaccgg
     1981 ccccacctcc tcgtggaacc gctgcgccgc ctgctccacg atcgaccaca gcaccgccta
     2041 cttggagggg atgtgggcgt agaggctgcc gccctggagg tcgagcgcgc gggcgatgtc
     2101 gcgcatgctg gtggcggcgt acccgcgctc gctgaacagc gagctcgcga cctcctcgat
     2161 ctgcctgcgg cgtgggcgca tgcgcagcgc tcctaacgaa cggttgttag gcacggtagc
     2221 agccgtcccg gcggccggca agtcgacccg gcaccggccg ccgggacgcc gggcggcggc
     2281 tgggcgtcag aggtcgagct cgaaccagac gacccgcccg cgggagccgc cgctcaagcc
     2341 ccatcggctc gccagcgcct cgaacaggtc caggccgcgg ccggtgaccg cgtcgagctg
     2401 gtggacccgg cggcgcggca cggtgggcgc gccggcgtca tggacctcga ccctgagccg
     2461 gcggtcgtcg tcgacctggc accgcacctc gaagctgccc ccctcgtcgc ccgatgccga
     2521 gtggagcagc gcgttggtcg cggcctcggt cacgagcagc cgggcggtct cctcggcgcg
     2581 ggaggcgcag gagcctcggg agagcacgcc gccgacgaag cgtcgagcgg cgccgacctg
     2641 gtcggcgctg ccggcgaagc gcttgcgcac gccccggaag cgctggccgg gggggcccca
     2701 tcctgcctcc attacccggc gatcatccca tctggcaggt acggatgact cgacatagcg
     2761 gcgctggatc tacccatcca ggcatgctca ggaaactcgt cctttctgcc tatctcgtga
     2821 gcggagggtg cgccgtcggg gactgatcgc gtccaggccg gtcagcgcgg cgtcggctgc
     2881 caggcaggcc gggagacgac cggcttgccg gtgggcaggc aggccgcccc cgttccgtcg
     2941 gcgcgggcga cgaagatgct gccgtcgcgc acgaaggcga ggcggccgcc atccggagac
     3001 caggacgggg cggcgtcccg ccggccgttg gacagttggc ggtcaccggt tccgtcggcg
     3061 tccacgacgt ggatcgcgcc gtccttgacg taggcgaggc gacggccgtc gggcgaccag
     3121 gcgggcgcgt actcgtagcc cgtggcggtg gtcaaccggt ggagcccgga ccc
//
LOCUS       DASPYY010000375         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_63358, whole genome shotgun sequence.
ACCESSION   DASPYY010000375 DASPYY010000000
VERSION     DASPYY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63358"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1063)
                     /locus_tag="VEP73_05800"
     CDS             complement(215..1063)
                     /locus_tag="VEP73_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT25979.1"
                     /translation="MDLGLEGRGVLVTGGSGGIGQAVCRVLAAEGARVAVHYHRRAHA
                     AEALAAELPGAVAVGGDLACEAGAESVVAAAEGTFGRLDVCVHAAGVWPEAAVPLWEM
                     RLERWEATMRADLTATFLVARAFLARVAARGAGNLVLVGSTAAVYGEAGHADYAAAKA
                     AMVYGLTRSLKNEIVQAAPAGRVNAVCPGWTVTPMTRSALADPTLVPRVTRTMALRKL
                     ATAEDVAWQVAVLASDRVSGHVTGQVVEVAGGMEGRVLHEGPGREGSQVGGAPAPAAG
                     GTARHP"
     gene            1139..1777
                     /locus_tag="VEP73_05805"
     CDS             1139..1777
                     /locus_tag="VEP73_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HYT25980.1"
                     /translation="MRGMDMGSGGVAGAVLAGGASRRMGRDKATLPVGGTALARLALA
                     AVAAVADPVALVAPAGHPAATLAAPSLRVADPGRGPLAALAATLRALDAEHVLVVAGD
                     HPGLRVELLRRLAAERAGAAAVACRRATGVEPLVAVYERAPALALAEARVAAGADRSL
                     RGLLAALGARVLDEAEWRPLDPDGSSFVDLDDPGDLAAWESGRWRRSAPGHW"
     gene            1818..2051
                     /locus_tag="VEP73_05810"
     CDS             1818..2051
                     /locus_tag="VEP73_05810"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HYT25981.1"
                     /translation="MEEFLEKVRGLLGPDAPTVGEGEVEAVLELARVAAHASERRAAP
                     VTAYMVGLAVAGAAPEAREAFLDDLVVKLEVQR"
     gene            complement(2100..>3053)
                     /locus_tag="VEP73_05815"
     CDS             complement(2100..>3053)
                     /locus_tag="VEP73_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HYT25982.1"
                     /translation="GATLAWTMNLARELQQRFDGTWWYAAAQQYADSLNDPGNQQSFQ
                     KHWIGQTPMEAELRAQGETVPGLAPFDHGNTALAGRENDCYSGDHPLNPGLFHTGCGG
                     GANGQGEKVIFGLTTSIQSIGEGNYGRLGPGQQQRYTHALAETMFSEPATGGTPDEQP
                     GAMPEIFPSPDQGANIDRCWTCRSMFMQAWGNYGTAWAVVHQWLGVRPDLGAGLLEVT
                     PQVPQGQTSVQGQNIRLGGGSVDVLASHAIGVYRTEIDTTAGVGANEVDIGHTLPRGA
                     RPAAVVLDGQAVHNYVVRETNRGTEVTVPTTAGHHTLTITV"
BASE COUNT          325 a         1147 c         1180 g          401 t
ORIGIN      
        1 tcgagcgggc tcgcgtacac ggcgtcgatc gccacggggg ccaggcgctc ggccagggcc
       61 ctggcctgct cgtggccctc gtcgctgagg tgcaggccag gcgtccagcc cgagagccgc
      121 gtgccggtgt ccgcggtgac cgcgtggcgg aagaggagga gcagatccat cgtgcggcta
      181 ccttcggggg tcggggcgcg gttcgactgc aattctacgg atgtcgggcc gtgccgcccg
      241 cggcgggcgc gggcgccccg cccacctggg acccctcgcg cccgggccct tcgtgcagga
      301 cccggccctc catgccgccg gcgacctcga ccacctgtcc ggtgacgtgg ccggagaccc
      361 ggtcggacgc gagcacggcc acctgccagg cgacgtcctc ggcggtggcg agcttgcgca
      421 gcgccatcgt gcgcgtcacc cgggggacca gggtggggtc ggccagggcc gagcgggtca
      481 tcggggtcac ggtccagccc gggcagacgg cgttgacgcg ccccgccggc gcggcctgga
      541 cgatctcgtt cttcagcgag cgggtcaggc cgtacaccat ggccgccttg gctgcggcgt
      601 agtcggcgtg gccggcctcg ccgtacacgg cggccgtgga gccgacgagc accaggttcc
      661 cggcccccct ggcggccacc cgagcaagga aggccctggc cacgaggaag gtggcggtga
      721 ggtcggcgcg catggtcgcc tcccagcgct cgaggcgcat ctcccagagc ggcacggccg
      781 cctcgggcca gacccccgcc gcgtggacgc acacgtcgag acggccgaat gtgccctcgg
      841 cggcggcgac gaccgactcg gcgcccgcct cgcaggcgag gtcgccgccg accgccaccg
      901 cgccggggag ctcggccgcg agcgcctcgg cggcgtgcgc ccgccggtgg tagtggaccg
      961 ccaccctcgc cccctcggcg gcgaggacgc ggcacacggc ctggccgatg ccgcccgagc
     1021 cgcccgtcac cagcacgccc ctgccctcga gtcccaggtc cacgctcgcc tccaccggtg
     1081 cgtcggccgc atgctggatc ggccgcatgc tggatcatag gaggggaaag gcggggacat
     1141 gcgcggcatg gacatgggct cgggcggcgt ggccggggcc gtgctggccg gcggggcgag
     1201 ccgccgcatg ggtcgcgaca aggcgaccct gccggtcggc gggaccgccc tcgcccggct
     1261 cgcgctcgcg gcggtggccg ccgtcgccga cccggtggcc ctggtcgccc ctgcggggca
     1321 tcctgccgcc accctcgccg cgccgtccct ccgggtcgcc gaccccgggc gggggccgct
     1381 cgcggcgctc gcggccaccc tgcgcgcgct cgacgccgag cacgtgctgg tcgtcgccgg
     1441 ggaccatccc ggcctgcggg tcgagctgct ccgccgcctg gccgccgagc gggcgggagc
     1501 cgccgcggtg gcctgccggc gcgccacggg cgtggagccg ctggtcgcgg tgtacgagcg
     1561 ggcgccggcg ctggcgctgg ccgaggcccg cgtggccgcc ggcgccgacc gctcgctgcg
     1621 cgggctgctc gcggcgctcg gcgcccgcgt gctcgacgag gccgagtggc gcccgctcga
     1681 cccggacggc agctccttcg tcgacctcga cgacccgggc gacctcgccg cctgggagtc
     1741 cgggcggtgg cgccggtccg ctccgggcca ctggtagggt gcgcgggaga ccggcgtgcg
     1801 aatgcctggg aggacccatg gaggagttcc tggagaaggt ccgcggcctg ctcggcccgg
     1861 acgcgccgac cgtcggcgag ggcgaggtcg aggccgtgct ggagctcgcc cgcgtggccg
     1921 cccacgcctc cgagcgccgc gccgcgcccg tcaccgccta catggtcggc ctggcggtcg
     1981 ccggcgcggc ccccgaggcg cgcgaggcgt tcctcgacga cctcgtggtc aagctggagg
     2041 tccagcgctg aggccgccgg ccgggacggc cggcggcccc ggggcctgga ggggctggtt
     2101 cagacggtga tggtcagggt gtggtgcccc gcggtcgtcg gcacggtcac ctcggtcccg
     2161 cggttggtct cgcggaccac gtagttgtgc accgcttgcc cgtcgagcac gacggccgcg
     2221 ggccgcgcgc cgcgcgggag ggtgtggccg atgtcgacct cgttggcgcc cacgcccgcg
     2281 gtggtgtcga tctcggtgcg gtagacgccg atggcatgtg aggccagcac gtcgacggac
     2341 ccgccgccga gccggatgtt ctgtccctgc accgaggtct ggccctgcgg cacctgcggc
     2401 gtcacctcga gcaggccggc gccgaggtcg ggccgcacgc ccagccactg gtgcacgacc
     2461 gcccacgcgg tgccgtagtt gccccacgcc tgcatgaaca tcgagcggca cgtccagcaa
     2521 cggtcgatgt tggcgccctg gtccggcgac gggaagatct cgggcatcgc tcccggctgc
     2581 tcgtccggcg tcccgcccgt ggcgggctcg gagaacatcg tctcggccag cgcgtgcgtg
     2641 tagcgctgct gctggcccgg gcccaggcgg ccgtagttgc cctcaccgat cgactggatc
     2701 gacgtcgtca ggccgaagat gaccttctcg ccctggccgt tggctccgcc tccacagccc
     2761 gtgtggaaca gcccggggtt gagcgggtgg tcgccgctgt agcagtcgtt ctcccgcccc
     2821 gccagcgcgg tgttcccgtg gtcgaacggg gccagtccgg ggaccgtctc gccctgggcg
     2881 cgcagctccg cctccatggg cgtctggccg atccagtgct tctggaagga ctgctggttg
     2941 cccgggtcgt tcagggagtc ggcgtactgc tgcgcggcgg cgtaccacca ggtgccgtcg
     3001 aagcgctgct gcagctcgcg ggcgaggttc atcgtccacg cgagggtggc ccc
//
LOCUS       DASPYY010000376         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_626968, whole genome shotgun sequence.
ACCESSION   DASPYY010000376 DASPYY010000000
VERSION     DASPYY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626968"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VEP73_05820"
     CDS             <1..502
                     /locus_tag="VEP73_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HYT25983.1"
                     /translation="SLDTVTIEDALRLLSLPRVVGTDPADGAEITAQNGRYGPYLKKG
                     TDSRSLLSEDALFTVGLEEALALFAQPKPRGRGRSVAPPLRELGADPGSGQPLVVREG
                     RFGAYVTDGETNATLRKADSVETLTVERAAELLAEKRAKGPAPKKRSAAPRKRAPAAP
                     RRPRRS"
     gene            604..1236
                     /gene="tmk"
                     /locus_tag="VEP73_05825"
                     /pseudo
     CDS             604..1236
                     /gene="tmk"
                     /locus_tag="VEP73_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018720737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
     gene            1233..>1657
                     /locus_tag="VEP73_05830"
     CDS             1233..>1657
                     /locus_tag="VEP73_05830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155476.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYT25984.1"
                     /translation="MSTSGVWADLVGQTRVVEQLRLAASSAREIVDGTGPGGAMTHAW
                     VFTGPPGSGRSTAALAFAAALQCTTTDEAPGCGHCHACRTALEGTHPDVEVVRPPGLH
                     HLTDDIRHLVREAVLSPANGRWQVVVVEDADRLTDQACNA"
BASE COUNT          230 a          612 c          595 g          220 t
ORIGIN      
        1 gtcgctggac accgtgacga tcgaggatgc gctgcggctg ctcagcctgc cgcgggtggt
       61 cggcaccgac cccgcggacg gcgccgagat caccgcgcag aacggtcgct acggccccta
      121 cctgaagaag ggcaccgact cgcgctcgct gctgtccgag gacgcgctgt tcacggtggg
      181 gctggaggag gcgctggcac tgttcgcgca gcccaagccg cgcggtcgcg ggcgctcggt
      241 cgccccgccg ctgcgcgagc tcggcgccga cccgggatcg gggcaaccgc tggtcgtgcg
      301 cgagggtcgg ttcggcgcgt acgtgaccga cggggagacc aacgcgacgc tgcgcaaggc
      361 cgactccgtc gagacgctga ccgtcgagcg cgccgcggag ctgctcgccg agaagcgggc
      421 caaagggccg gcacccaaga agcgttccgc cgcaccgcgc aagcgtgccc ccgccgcccc
      481 ccgtcgtccc cgtcgatctt gagcaacact cctgcgactc gccgggcggg ccggtgtgtt
      541 gctcaagatc aacaagggag gaccgggtgg tcacggggcg cggatagggt ttccgtcatg
      601 cgcgcaggcc tgttcgtcgc cttcgaaggt ggtgaggggg ccggcaagtc cacccagatc
      661 accgctctcg cagcgctgct gcgcgcccgc ggcctcgaag tgctggagac ccacgagccc
      721 ggcgacaccc ggatcgggcc ggacgtgcga cggctggtgc tcgggcatgc cagcacgggc
      781 ctgtccgcca aggccgaggc gctgctctac gccgccgacc gggccgagca cgtcgacgcg
      841 gtgatccgtc cggcactggc cgcgggcagc gtcgtcctca ccgaccgcta cgtcgactcg
      901 tcgctcgcct atcagggcgc cggccgtccg ctcgactacg acgaggtcgc gcgcatcaac
      961 gcctgggcaa ccggtggtct gctccccgac ctgaccgtgc tcctcgacct cgaccccgcc
     1021 cagggcctgc gccgcgcggg cgaacccgac cgcctcgagg tcgagccgct ggagttccac
     1081 cgcagggtgc ggaccaagtt cctcgagctt gcggcagccg agccgtcccg ctacgtcgtc
     1141 gtcgacgccg ccgccgaggc cgagcacgtt caggacgagg tggctcgcgc agtgctcgcc
     1201 gtgttggccc ggctggatgc gtcggtggcg ccatgagtac gagcggggtg tgggccgacc
     1261 tggtcgggca gacccgggtc gtcgaacagc tccgactcgc cgcttcgtcc gcgcgcgaga
     1321 tcgtcgacgg aacaggtcct ggcggcgcca tgacccacgc ctgggtcttc acgggcccgc
     1381 ccgggtcagg acgcagcacc gcggcgctgg cgttcgcggc ggcgctgcag tgcacgacga
     1441 ccgacgaggc acccgggtgc ggtcactgcc acgcctgccg tacggccctc gaaggcaccc
     1501 acccggacgt cgaggtcgtg cggccgccgg gtctgcacca tctcaccgac gacatccgcc
     1561 atctcgtacg ggaggcggtg ctctcgccgg caaacggccg ctggcaggtc gtcgtggtcg
     1621 aggacgccga ccggctcacc gaccaggcct gcaacgc
//
LOCUS       DASPYY010000377         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1750024, whole genome shotgun sequence.
ACCESSION   DASPYY010000377 DASPYY010000000
VERSION     DASPYY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750024"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VEP73_05835"
     CDS             <1..682
                     /locus_tag="VEP73_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25985.1"
                     /translation="GGGAMIPPMPPGPATPSGEVPDEVARRWRAAEEQLYPVVMTRPD
                     LYERSIRLVRAVADELRPLATPAALLATYAGAAELVAGAIQRRGLPVEGLDLGLATDA
                     AFSLRYREVLGEVAREQAMERMRTARERGDEWVLLHESGTLTHAPAVPYRRLEMRLRD
                     GAGLHVFVQQDPATAGPLYGVEAVQLDPHTGDWLTDAAPWARPETFSQPEPWQRLVEE
                     LRGRRQDP"
     gene            773..1633
                     /locus_tag="VEP73_05840"
     CDS             773..1633
                     /locus_tag="VEP73_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT25986.1"
                     /translation="MASERVPRRVIDGHSHIGEMAAWRFYDLKEAVKPTVYEFAGTGD
                     YLAHLDRLGVERALVISNYGIPQQEQPFSLNPLVLDSVEASDRLCGALWVSFLPQNRE
                     RTLEALKHAGEARVVALKTTFLLGGNPNPETWDEETAALAEACFTAAEEHDLVFHFHT
                     SPGGASDISNFIPLVERYGKRVRLYLVHFGGGVSGHIRLVPRFLDWVEEGYRVYTDTT
                     WSIGFGPRWLLSEIERRGVGHDRVIFASDEPWSDFWGEYWKIAGAPASEELKERVFHQ
                     NFEDLYGHRW"
     gene            1617..>2399
                     /locus_tag="VEP73_05845"
     CDS             1617..>2399
                     /locus_tag="VEP73_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYT25987.1"
                     /translation="MDIGGDARPVMSPELGRVITELQSEGLRVEQPVETRRGGAGPSD
                     AGMIWIEGIPVTVPVGAEYVAGSPFVLRQEDQGWGIYRRGQRVASADPLQRPRFYDLS
                     TADGVPYWKIALLHLDSLASTVLQTCAYWGTDDQCRFCGIELSLEAGRTIAVKRPEQL
                     AEVAVAAKELDGVVDVTLTTGSTHGPDRGARYLGRCAGAIKRASGLPVEGQFEPPPDL
                     GVLDEVHDAGLDSVGMHVESFDPQVLARVAPAKARTGIDGYFQ"
BASE COUNT          342 a          864 c          868 g          325 t
ORIGIN      
        1 cggcggaggc gcgatgatcc ctcccatgcc tcccggtccc gcgaccccct cgggcgaggt
       61 cccggacgag gtcgcccgcc gctggcgtgc cgccgaggag cagctctacc cggtcgtcat
      121 gacccggccc gacctctacg agcgcagcat ccggctggtg cgggccgtgg ccgacgagct
      181 gcgtccgctc gccaccccgg cggcgctgct ggcgacgtac gcgggtgccg ccgagctcgt
      241 ggccggtgcg atccagcggc ggggcctgcc cgtcgagggt ctggacctcg ggctcgcgac
      301 cgatgccgcg ttcagcctgc gctaccgcga ggtccttggc gaggtcgccc gggagcaggc
      361 catggagcgg atgcggacgg ccagggagcg gggtgacgag tgggtgctcc tgcacgagag
      421 cggcaccctc acccacgcgc cggcggtgcc ctaccgccgc ctcgagatgc gcctccggga
      481 cggcgccggc ctgcacgtgt tcgtccagca ggacccggcc acggccgggc cgctgtacgg
      541 ggtggaggcg gtgcagctcg acccgcacac cggcgactgg ctgaccgacg cggccccgtg
      601 ggcgcggccg gagaccttca gccagccgga gccctggcag cggctggtcg aggagctgcg
      661 cgggcgccgg caggaccctt gacacaccct ccgcaccgtg gagaatttcc tttagtagat
      721 gccactcttc caaaagtgat cggctccggc cggtcgagcg agaggtgggc aggtggccag
      781 cgagcgagtc ccccggcgag tgatcgacgg ccatagccac atcggagaga tggcggcgtg
      841 gcgcttctac gacctgaagg aggcggtcaa gcccacggtc tacgagttcg ccggcaccgg
      901 ggactacctg gcccacctgg accgcctcgg cgtcgagcgg gcgctggtga tctccaacta
      961 cgggatcccc cagcaggagc agccgttctc gctgaacccg ctggtgctcg actcggtcga
     1021 ggcctccgac cggctctgcg gcgcgctctg ggtctcgttc ctcccgcaga accgggagcg
     1081 gaccctcgag gcgctgaagc acgcgggcga ggcacgcgtc gtcgccctca agacgacctt
     1141 cctgctcggc ggcaacccga acccggagac ctgggacgag gagacggccg cactcgccga
     1201 ggcgtgcttc acggccgccg aagagcacga cctggtgttc cacttccaca ccagccccgg
     1261 cggcgcctcc gacatcagca acttcatccc cctggtggag cggtacggca agcgggtgag
     1321 gctctacctg gtccacttcg gcggcggcgt cagcgggcac atccggctgg tgccgcggtt
     1381 cctcgactgg gtcgaggagg gctacagggt ctacaccgac accacctgga gcatcggctt
     1441 cggcccccgc tggctgctca gcgagatcga gcgccgcggc gtcggccacg accgggtgat
     1501 cttcgcctcc gacgagccgt ggagcgactt ctggggcgag tactggaaga tcgccggcgc
     1561 ccccgcgagc gaggaactga aggagcgggt cttccaccag aacttcgagg acctgtatgg
     1621 acatcggtgg tgacgcccgg cccgtgatgt cgccggagct cggcagggtc atcaccgagc
     1681 tgcagagcga aggcttgcgg gtcgagcagc cggtggagac ccggcggggc ggtgccggcc
     1741 cctccgacgc cgggatgatc tggatcgagg ggatcccggt gaccgtcccg gtgggcgccg
     1801 agtacgtcgc cggctccccc ttcgtgctcc ggcaggagga ccagggctgg gggatctacc
     1861 gacgcgggca gcgggtcgcc tcggccgacc cgctgcagcg accccgcttc tacgacctca
     1921 gcaccgccga cggcgtcccc tactggaaga tcgcgctgct ccacctcgac tcgctggcca
     1981 gcaccgtgct ccagacctgc gcctactggg gcaccgacga ccagtgccgc ttctgcggca
     2041 tcgagctgtc gctcgaggcc ggccggacga tcgccgtgaa gcgcccggag cagctcgccg
     2101 aggtcgccgt ggccgccaag gagctcgacg gcgtggtgga cgtgacgctc acgaccggca
     2161 gcacccacgg ccccgaccgc ggtgcgcggt acctgggccg ctgcgccggc gcgatcaagc
     2221 gggcgagcgg gctgcccgtc gaggggcagt tcgagccccc gcccgacctg ggcgtcctgg
     2281 acgaggtcca cgacgcgggc ctggactcgg tcgggatgca cgtggagtcg ttcgacccgc
     2341 aggtgctggc ccgcgtcgcg ccggccaagg cccgcaccgg catcgacggg tacttccag
//
LOCUS       DASPYY010000378         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1329169, whole genome shotgun sequence.
ACCESSION   DASPYY010000378 DASPYY010000000
VERSION     DASPYY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329169"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VEP73_05850"
     CDS             <1..1497
                     /locus_tag="VEP73_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYT25988.1"
                     /translation="RVGEALREALDGVAVSLSSEVLPEFREYERFSTTVADAYLTPIL
                     AAYLERLAGEATGRGLPAPLVMQSSGGVIEVEAAAAHAASCVLSGPAGGVVGAAYVAK
                     LSGYDDLLTFDMGGTSTDVAPVVGGEVRTTTESVVASVPIKLPMVDVHSVSAGGGSIA
                     WVDEGGALRVGPRSAGADPGPAAYGRGGEEPTVTDANLYLGYLADGAELGGELTLRRD
                     LADRALARVGGRLGEDALGVALGAVRVANAEMVRALRVISVERGLDPREFTLVAFGGA
                     GGLHACALAEEMGMRRVLVPAASGVLSALGLAISDLRRDYVRPLLGRLGGVDPGEAER
                     AFGEMEEAAARDLNDPETERRADLRYRGQSFELTVGAGGWDGLEERFHAEHERRYGFR
                     MDDETVELVSLRLLATVAGDKPELRAEAPGTDREPGRREVNLDGEWQEVEVVRRRDLD
                     AGAKLDGPAVVEFAEATLLLRPGWRATLDDVGTLVLERDREEGGRWTA"
     gene            1542..3131
                     /locus_tag="VEP73_05855"
     CDS             1542..3131
                     /locus_tag="VEP73_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYT25989.1"
                     /translation="MGSLLVRSAYSSNIKERRDCSTALFDARGRMVAQAEHIPVHLGA
                     MPEAVAAVMARDPEPGDVFAINDPYSGGTHLPDVTLVSQLAVDGEVMGWAVTRAHHSD
                     VGGMRPGSMPSDSREIWQEGLVIPPVRLVRGGGYVGDVLDLILANSRTPALRRGDFRA
                     QVAANRLAGERLAELVRRRGRDTVLEAFDEVIAYAERRTRDAIEALADGEYTAEGEIE
                     GDGVTDDDIPIRVKVTVAGDEMTIDLGGTAGAVAGNVNCPLAVTRSACYFALRVLLPN
                     DVPANAGTYAPLTILAPEGSLVHAVSPAAVVAGNVETSQRVADTVLAALARCADLPAQ
                     GQGTMNNLVIGGRGWTYYETIAGGQGASSAGPGPSGVHVGMTNTLNTPIEALELEYPM
                     RVERYELVDGSGGAGRNRGGDGVVRAVRVLEPASLSLLTDRRRHAPAGARGGAPGAPG
                     RNLLDGEELPPKASRELPAGAVVTVVTPGGGGMGDPAGGGAGAAEEELAGSRGKPRPV
                     GRRPDRPAHPAESEVYLRVHG"
BASE COUNT          396 a         1157 c         1260 g          382 t
ORIGIN      
        1 cgggtcggcg aggcgctgcg ggaggcgctc gacggggtgg cggtgtcgct ctcctccgag
       61 gtgctgcccg agttccggga gtacgagcgg ttctccacca cggtggccga cgcctacctg
      121 accccgatac tcgccgcgta cctcgagcgc ctggccggcg aggcgaccgg gcgggggctg
      181 cccgcgccgc tcgtcatgca gtcctcgggc ggggtgatcg aggtggaggc ggcggctgcc
      241 cacgccgcga gctgcgtgct ctccgggccg gcgggcgggg tcgtcggggc cgcgtacgtg
      301 gccaagctct ccggctacga cgacctgctg accttcgaca tgggcgggac ctccaccgac
      361 gtcgcgcccg tggtcggcgg cgaggtgcgg accaccaccg agtcggtggt cgccagcgtg
      421 ccgatcaagc tgccgatggt cgacgtccac agcgtgagcg ccggcggcgg ttcgatcgcc
      481 tgggtggacg agggcggcgc cctgcgggtc gggccccgct cggccggcgc cgaccccggg
      541 ccggccgcgt acggcagggg cggcgaggag ccgaccgtga ccgacgcgaa cctctacctc
      601 ggctacctgg ccgacggcgc cgagctgggc ggggagctca ccctccggag agacctggcc
      661 gatcgggcgc tggcccgggt cggcgggcgg ctcggcgaag acgccctcgg cgtggcgctc
      721 ggcgccgtcc gggtggccaa cgccgagatg gtcagggccc tgcgggtgat cagcgtcgag
      781 cggggcctcg acccgcgcga gttcaccctg gtggccttcg gcggcgccgg cggcctgcac
      841 gcctgcgccc tcgccgagga gatgggcatg cggcgcgtgc tcgtcccggc cgcgagcggg
      901 gtgctgagcg cgctcggcct cgccatctcc gacctgcgcc gcgactacgt gcgcccgctg
      961 ctcggccggc tcggcggggt cgacccgggc gaggcggagc gtgccttcgg cgagatggag
     1021 gaggcggccg cgcgcgacct caacgacccg gagaccgagc gccgggccga cctccgctac
     1081 cgcggccagt ccttcgagct gaccgttggc gcgggcgggt gggacgggct cgaggagcgg
     1141 ttccacgccg agcacgagcg gcgctacggc ttccggatgg acgacgagac ggtcgagctg
     1201 gtgagcctca ggctgctagc gaccgtggcc ggcgacaagc ccgagctccg cgccgaggcg
     1261 ccgggcaccg accgcgagcc cggccgccgg gaggtcaacc tcgacggcga gtggcaggag
     1321 gtcgaggtgg tgcggcggcg ggacctcgac gcgggcgcga agctcgacgg gccggcggtg
     1381 gtcgagttcg ccgaggcgac cctgctgctc cggccgggct ggcgggcgac cctcgacgac
     1441 gtcggcaccc tggtcctcga gcgcgaccgg gaggagggag gccggtggac ggcgtgaccc
     1501 tttccgtgct ggtcagctcg ctcaccggca tcgccgagga gatgggctcg ctgctcgtgc
     1561 gcagcgccta ctcctcgaac atcaaggagc gccgggactg ctcgaccgca ctgttcgacg
     1621 ctcgcgggcg gatggtcgcc caggccgagc acatcccggt ccacctgggc gccatgccgg
     1681 aggcggtggc ggcggtgatg gcgcgcgacc ccgagccggg cgacgtcttc gcgatcaacg
     1741 acccgtactc gggcggcacg cacctgcccg acgtcaccct ggtctcccag ctcgcggtcg
     1801 acggcgaggt catgggctgg gcggtcaccc gggcgcacca ctccgacgtc ggcgggatgc
     1861 gcccagggtc gatgccgagc gactcgcgcg agatctggca ggagggcctg gtcatcccgc
     1921 ccgtgcgcct cgtccgcggc ggcgggtacg taggtgacgt gctcgacctg atcctcgcca
     1981 actcgcggac cccggcgctg cggcggggcg acttccgcgc ccaggtcgcg gccaaccggc
     2041 tggccggcga gcggctggcc gagctcgtcc ggcgccgcgg ccgcgacacg gtgctcgaag
     2101 cgttcgacga ggtcatcgcc tacgccgagc ggcgcacgag ggacgcgatc gaggcgcttg
     2161 cggacggcga gtacaccgcg gagggcgaga tcgagggcga cggcgtcacc gacgacgaca
     2221 tcccgatccg ggtcaaggtg accgttgccg gcgacgagat gaccatcgac ctcggcggca
     2281 cggccggcgc ggtcgccggc aacgtcaact gcccgctcgc ggtcacccgg tccgcctgct
     2341 acttcgcgct gcgggtgctg ctccccaacg acgtcccggc caacgccggc acctacgcgc
     2401 cgctcacgat cctcgccccc gaggggagcc tggtgcacgc cgtctccccc gcggcggtgg
     2461 tggcgggcaa cgtggagacg agccagcgcg tggccgacac ggtgctggcc gccctcgcgc
     2521 ggtgcgccga cctccccgcc cagggccagg ggacgatgaa caacctcgtc atcggcggcc
     2581 ggggctggac ctactacgag acgatcgcgg ggggccaggg cgcgtcgagc gccggcccag
     2641 ggccgtccgg cgtccacgtc ggcatgacga acacgctcaa cacgccgatc gaggcgctcg
     2701 agctcgagta ccccatgcgg gtcgagcgct acgagctggt ggacgggtcg ggcggcgcgg
     2761 ggcgcaaccg gggcggcgac ggggtggtcc gcgcggtccg cgtcctcgag ccggccagcc
     2821 tctcgctgct caccgaccgc cgccgccacg ctccggccgg cgcccgcggc ggcgcgccgg
     2881 gcgcacccgg ccgcaatctc ctcgacggcg aggagctgcc gccgaaggcg agccgcgagc
     2941 tgcccgcggg cgcggtggtg accgtcgtca ccccgggcgg cgggggcatg ggcgatccgg
     3001 ccggcggagg cgcgggcgcc gccgaggagg agctggccgg ctcgcggggg aaacctcggc
     3061 cggtcggccg gcggccggat cgcccggcgc atccggccga atcggaggtg tatctgcgcg
     3121 ttcatgggta agaccggcgg ggacggcagg gaagagccat ccccgtcacc gctctggagg
     3181 cgctatgacc ctacc
//
LOCUS       DASPYY010000379         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2733677, whole genome shotgun sequence.
ACCESSION   DASPYY010000379 DASPYY010000000
VERSION     DASPYY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733677"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VEP73_05860"
     CDS             complement(<1..228)
                     /locus_tag="VEP73_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYT25990.1"
                     /translation="MPDSLSAAIYVRISRDREGRQIGVRNQEPPCRELATRLGWTVGR
                     VFIDNDLTAYHERRRPEYEEMLAYVRDGRVRG"
     gene            277..564
                     /locus_tag="VEP73_05865"
                     /pseudo
     CDS             277..564
                     /locus_tag="VEP73_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            547..915
                     /locus_tag="VEP73_05870"
     CDS             547..915
                     /locus_tag="VEP73_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25991.1"
                     /translation="MQVDRVGAEELEAAGAGEASGPVRARVCAARAVQQARSGVENAR
                     LPVAGLTLACRPTAAARRTLAAAVDRLGLTARGFHRALRVSRTIADLRGDERVDDDHV
                     REALALRRAAVAGDPEGAGS"
     gene            912..2111
                     /locus_tag="VEP73_05875"
     CDS             912..2111
                     /locus_tag="VEP73_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYT25992.1"
                     /translation="MSHRRRPRAAGLPPWWSPERPFREVLLALAATPGPLPAVTADRL
                     AQGLAPDDLLRPDSPDPARVAATLEALGQRFVLPGDPAWPLAATPPEPPCAWLFVAGT
                     VPPGPAWSVAVVGGRRASPLRLAVARSLGAGLARAGRCVVSGGAAGADAAAHHGALDA
                     GGRTVAVLGCGLEVAYPRSNAVLFHRILEAGGGLVGEHPPGGRPRAAHFPPRNRLISA
                     LAGAVVVVEAGSRSGSLTTARAAGGRGGGRLLAVPGAPWDQGATGCNDLIRDGATLVR
                     GLDDVLAELELAPAGGAPELPLRRWPAGAGPAARAVLDALVDGQVMGTARLAALTGLP
                     PEPLAVAVVELELAGFVRRLAGGLQAVALPLAAPRGGHGPPVPRPRVSGAAGPGGGTP
                     APGGPGG"
     gene            2123..3070
                     /locus_tag="VEP73_05880"
     CDS             2123..3070
                     /locus_tag="VEP73_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HYT25993.1"
                     /translation="MSGTEAATDDLAPGVRRAVDLFVSHLRDERGLSANTVAAYECDV
                     VQFFRFAGRAGVTDPGGVEPLLLRRFLALLRTRGLAASSMARKGAALRACFRYLARRG
                     VVAADPAADLGTPRGPSRLPVVLKQRQVDELLARPEPVDPVGLRDRAVLELLYATGVR
                     VGELCGLRLDDVDLGGDEVRVTGKGARQRVVPFGAPARAALVAYLAHGRSRMLPAPGE
                     AAGAADARALFFNRRRRPMTTRDVRGMLERYRVGVAPPGTSPHTLRHSFATHLLEGGA
                     DLRAVQELLGHVALTTTQTYTHVSKERLRQVYEQAHPRA"
     gene            3179..3946
                     /gene="whiG"
                     /locus_tag="VEP73_05885"
     CDS             3179..3946
                     /gene="whiG"
                     /locus_tag="VEP73_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HYT25994.1"
                     /translation="MDEPSSVERLWQEFKLKASSDARERLILHYAPLVKYVAGRVSVG
                     LPPNIEQADLVSYGIFGLIDAIEKYDLDRGIKFETYAISRIKGAIIDELRAIDWIPRS
                     VRFKAREVEKAYASLENRLHRSPTDAEVAQELGISTSELHHIFNQISFVSVVALDELL
                     TVGSERGDRLSLVDTLEDKGAEDPEAAFESEEMKSILAGAINRLAEREKIVITLYYYE
                     GLTLAEIGQVLGVTESRVCQMHTKAVLQLRGKMQDAR"
BASE COUNT          471 a         1578 c         1611 g          544 t
ORIGIN      
        1 gccacgcacc cggccgtccc gcacgtaggc aagcatctcc tcgtactcgg gccgacggcg
       61 ctcgtggtag gcggtcaggt cgttgtcgat gaagactcgg ccaaccgtcc agccaagccg
      121 ggtcgcgagc tcgcggcaag gcggctcctg gttcctgaca ccgatctggc ggccctcccg
      181 gtcacgggag atgcgcacat agatcgcggc ggagaggctg tccggcatag cggcagtgta
      241 cctcactcta gggggtactt ctgaactcta gctcaccgtg gcgtgctctt cctcgacgag
      301 ctggccgagt tccccagggc tgcgtgcgag gcgctccgcc agccgctcga ggacggcgag
      361 gtgacgatcg tgcgcgcggg cgccagcgtg cggttcccgg cccgcttcgc cctggtcgcc
      421 gcggccaacc cgtgcccgtg cgggcatctc ggggacgcgg ccctggcctg ccggtgcggc
      481 ccggaggacc tgcgcaggta cgaccggcgg ctgtcaggcc cgctcctgga ccgcgtcgac
      541 ctctacgtgc aggtcgaccg cgtcggcgcc gaggagctcg aggccgccgg cgccggggag
      601 gcgtctgggc cggtccgcgc ccgggtgtgc gccgcccgag ccgtccagca ggcccgctcg
      661 ggggtcgaga acgcccgact gccggtggcc ggcctgaccc ttgcctgccg gccaacggcc
      721 gccgcccgcc gcaccctcgc cgccgcggtc gaccggctcg ggctcaccgc gcgcggcttc
      781 caccgcgccc tccgggtcag ccggaccatc gccgacctgc gcggcgacga gcgggtcgac
      841 gacgaccacg tccgcgaggc gctcgccctg cgccgtgccg ccgtcgccgg cgacccggag
      901 ggagccggct cgtgagccac cgccgccggc ccagggcggc cggactgccg ccgtggtggt
      961 cgccggagcg acccttccgg gaggtcctgc tcgcccttgc ggcgacgcca gggccgctgc
     1021 ccgcggtcac cgccgaccgg ctcgcgcagg ggctcgcgcc cgatgacctg ctccggcccg
     1081 acagcccgga ccctgcccgg gtggcggcca cgctggaggc gctcggccag cgcttcgtgc
     1141 tccccggcga cccggcgtgg cccctggccg cgacgccccc cgagccgccc tgcgcctggc
     1201 tcttcgtcgc ggggacggtc ccgcccggcc cggcgtggtc ggtggccgtg gtcggtggcc
     1261 ggcgcgcgtc gcccctgcgg ctcgccgtgg cccgatcgct cggcgccggg ctcgccaggg
     1321 cgggccgctg cgtcgtcagc gggggagcgg ccggggcgga cgcggccgcg caccacgggg
     1381 cactcgacgc cgggggccgg accgtcgccg tcctcggctg cggtctcgag gtcgcctacc
     1441 cgcggtccaa cgccgtgctg ttccaccgca tcctcgaggc gggcggcggc ctggtcggcg
     1501 agcacccgcc gggcgggagg ccccgcgccg cgcacttccc tccccggaac cgcctgatca
     1561 gcgcgctcgc cggggccgtc gtggtggtcg aggcaggctc ccggtcgggg agcctgacga
     1621 cggcgcgggc ggcgggcggc cgcggcggcg ggcggttgct cgccgtcccc ggggcgccgt
     1681 gggaccaggg cgcgaccggc tgcaacgacc tcatccggga cggcgcgacc ctggtccgcg
     1741 ggctcgacga cgtgctcgcc gagctcgagc tggcccccgc gggtggggcg ccggagctcc
     1801 cgctgcggcg ctggccggcg ggcgccgggc cggccgcccg ggcggtgctc gacgcgctcg
     1861 tcgacgggca ggtcatgggg acggcgcgtc tcgccgctct gaccggcctg ccgccggagc
     1921 cgctggcggt ggccgtcgtc gagctggagc tggccgggtt cgtccgccgg cttgccggcg
     1981 gcctgcaggc ggtcgccctg ccgctcgccg cgccacgcgg cggccacggg ccgcccgtcc
     2041 cccgcccccg cgtctccggt gcggccgggc ccggcggagg gacgcctgcg cccggcgggc
     2101 ctggcggcta ggctgtgcct cgatgagcgg gactgaagcg gcgaccgacg acctcgcccc
     2161 cggagtcagg cgggccgtgg acctgttcgt ctcgcacctg cgggacgagc gtgggctgtc
     2221 ggcgaacacg gtggccgcct acgagtgcga tgtcgtccag ttcttccggt tcgcggggcg
     2281 ggccggggtg accgacccgg gcggggtcga gccgctcctg ctccgccggt tcctcgccct
     2341 gctgcggacc agggggctgg ccgcctcgtc gatggcgcgc aagggtgccg cgctgcgggc
     2401 ctgcttcagg tacctggccc ggcgtggcgt cgtggccgcc gacccggccg ccgacctggg
     2461 gaccccccgg ggcccgagcc ggctcccggt ggtgctgaag cagcgccagg tcgacgagct
     2521 gctggccagg ccggagccgg tcgacccggt cggcctgcgc gaccgtgcgg tgctcgagct
     2581 gctgtacgcg accggggtgc gggtcggcga gctgtgcggg ctgcggctgg acgacgtcga
     2641 cctcgggggc gacgaggtcc gcgtcaccgg caagggcgcc aggcagcggg tcgtgccctt
     2701 cggcgcgccg gcgcgggccg cgctggtggc gtacctggcg catggccgct ccaggatgct
     2761 gccggcgccg ggcgaggcgg ccggcgcggc cgacgcgcgg gcgctgttct tcaaccggcg
     2821 ccgccgcccg atgaccacaa gggacgtgcg tggcatgctc gagcgctacc gggtgggcgt
     2881 cgcgcccccc ggcacgagcc cgcacacgct gcgccacagc ttcgccacgc acctgctgga
     2941 gggcggggcg gacctgcggg ccgtgcagga gctgctcggg cacgttgccc tcaccacgac
     3001 ccagacctac actcacgtgt cgaaagaacg actccgccag gtctacgagc aggcccatcc
     3061 gcgagcctga cgccgccgtc cggcagggcg gcgggcaggt cgctgcggcg gatcgggggg
     3121 gcggaaacgt cggacgccgg gccgccggcc gtccgtcgtc atatctaggg ggggcttttt
     3181 ggacgagccg tcgtccgtcg aacggctgtg gcaggagttc aagctgaagg cgtcgtccga
     3241 cgcccgtgag cggctcattc tccactacgc gcccctggtg aagtacgtcg ccgggcgggt
     3301 gtcggtcggg ctgccgccca atatcgagca ggccgatctg gtgtcctacg gaattttcgg
     3361 tctcatcgac gccatcgaga aatacgacct cgaccggggc atcaagttcg agacctatgc
     3421 catttcccgg atcaagggcg cgatcatcga cgagctgcgg gcgatcgact ggatcccccg
     3481 gtcggtgcgg ttcaaggccc gcgaggtcga gaaggcctac gccagcctcg agaaccggct
     3541 gcaccgcagc ccgaccgacg ccgaggtcgc ccaggagctc gggatctcga cctcggagct
     3601 gcaccacatc ttcaaccaga tctcgttcgt gtccgtggtg gccctggacg agctgctcac
     3661 ggtggggtcc gagcgggggg accggctcag cctggtggac acgctcgagg acaagggcgc
     3721 cgaggacccg gaggccgcgt tcgagtccga ggagatgaag agcatcctcg cgggcgcgat
     3781 caaccgcctg gccgagcgcg agaagatcgt catcacgctg tactactacg aggggctgac
     3841 ccttgccgag atcggccagg tgctcggcgt gaccgagtcg cgggtgtgcc agatgcacac
     3901 caaggcggtc ctgcagctcc gcggcaagat gcaggacgcg cgctgagcgc ggacggcggc
     3961 ccgccgcagt ccacagatcc gcgccaggcg ccaccagggc cgcggcgggc gtcgtagcct
     4021 gcggtcggtc cacccgcgag gcctgccggc cttgcccacg cccagaggga tgccgtgcac
     4081 ctgaagacga ccgtgccgtg cgcgctcgcc gccctgctcg cgctcgctgt ggccaccgcg
     4141 ccggctgccg cggggctggc gagcccctcg gcagcctcga ccgcccgcag cgcggggccg
     4201 gccg
//
LOCUS       DASPYY010000380         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_907463, whole genome shotgun sequence.
ACCESSION   DASPYY010000380 DASPYY010000000
VERSION     DASPYY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907463"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VEP73_05890"
     CDS             complement(<1..1111)
                     /locus_tag="VEP73_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYT25995.1"
                     /translation="MKAVIMAGGEGTRLRPLTANQPKPMLPLANRPMMEHIVRHVRDH
                     GVKDIVVTVQFLASQVRNYFGDGADMGVDLTYATEQRPLGTAGSVRNAAEQLQETFVV
                     VSGDALTDIGLDDVVAFHRRSGALGTVVTKRMENPLEFGIVIATEDGRIERFLEKPHW
                     GQVFSDTVNTGIYVLEPEVFDQIPEGGPVDFAADVFPKLLAMGAPLYAWVADGYWQDV
                     GSLEAYRHAHEDILEGRVRVDVRGFEVRPGVWLGEGAEVDPDAVLVAPVLIGDHAKVE
                     KGVRMREYSVVGANVVVKANAFLHRAIVHDNAYVGPGASLRGCVVGRGADVKRGGRVE
                     DGVVVGDNANIGRGAVLQPGVKVYPFKTVDPGAVVT"
     gene            complement(1189..1893)
                     /locus_tag="VEP73_05895"
     CDS             complement(1189..1893)
                     /locus_tag="VEP73_05895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYT25996.1"
                     /translation="MSKPNGPEPARRGPAADAPAAGPGEAQVAAAPGPLDAIGPADAA
                     QPPSTAILTAPNAISSARLLLMPVCAWQLGTGHYAIGLWLTAVVGSTDWVDGWLARRT
                     GRVSRLGQLLDPLADRLLIASVAVALVVRGVLPLLAVALLVGRDVLLLAGFPLLRSRG
                     VRPPDVVWVGKAATFDLLLALPVLVVGETHLALAPAAHVIGLILLWAGVVLYYVAGVV
                     YVRMALEGLRAARASA"
     gene            complement(1980..2057)
                     /locus_tag="VEP73_05900"
     tRNA            complement(1980..2057)
                     /locus_tag="VEP73_05900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2020..2022),aa:Pro,seq:ggg)
     gene            complement(2143..>2340)
                     /locus_tag="VEP73_05905"
     CDS             complement(2143..>2340)
                     /locus_tag="VEP73_05905"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT25997.1"
                     /translation="GKAVKVRYVTQARVAPPEFVFFATGVLAPAYRRYLEHGLRRTFG
                     FQGTPVRVIGRVRTRERRDDA"
BASE COUNT          327 a          864 c          855 g          294 t
ORIGIN      
        1 tggtcacgac cgcgccgggg tcgaccgtct tgaacgggta gaccttgacc ccgggctgca
       61 ggaccgcccc ccggccgatg ttcgcgttgt cccccacgac cacgccgtcc tcgacccgcc
      121 cgccccgctt cacgtcggcg ccgcggccga cgacgcagcc gcgcaggctg gcgccgggcc
      181 cgacgtaggc gttgtcatgg acgatggccc ggtggaggaa ggcgttcgcc ttgacgacca
      241 cgttcgcgcc gacgacgctg tactcccgca tgcgcacgcc cttctccacc ttggcgtggt
      301 cgccgatcag gacgggcgcg acgaggacgg cgtccgggtc gacctcggcc ccctcgccga
      361 gccatacccc gggccggacc tcgaaccccc gcacgtccac ccgcacccgg ccctccagga
      421 tgtcctcgtg ggcgtggcgg tatgcctcca ggctgccgac gtcctgccag tagccgtcgg
      481 ccacccaggc gtagagcggg gcgcccatcg cgagcagctt cgggaagacg tcggcggcga
      541 agtcgaccgg gccgccctcg gggatctggt cgaacacctc gggctcgagc acgtagatcc
      601 cggtgttgac ggtgtcggag aacacctggc cccagtgggg cttctcgagg aagcgctcga
      661 tgcggccgtc ctcggtcgcg atcacgatcc cgaactcgag gggattctcc atccgcttcg
      721 tgaccaccgt gccgagcgcc ccggagcggc ggtggaaggc cacgacgtcg tccaggccga
      781 tgtcggtgag ggcgtccccc gagacgacca cgaacgtctc ctggagctgc tcggccgcgt
      841 tcctcacgct ccccgcggtg ccgagcggac gctgctcggt cgcgtaggtc aggtcgacgc
      901 ccatgtcggc gccgtcgccg aagtaattgc gcacctggct ggccaggaac tgcacggtga
      961 ccacgatgtc cttgacgccg tggtcgcgga cgtgccggac gatgtgctcc atcatcggcc
     1021 ggttggcgag cgggagcatc ggcttcggct ggttggcggt cagcggccga aggcgggtgc
     1081 cctcgccgcc ggccatgatc acggccttca tggggcggcc tcctcacttg cacgtggagc
     1141 gctgccacgc tcccggtcgc ctcggtggat gggttcgccg acggcccgtc aggcgctggc
     1201 gcgggccgct cgcagcccct ccagcgccat tctcacgtag accaccccgg cgacgtagta
     1261 gaggacgacc cccgcccaca gcaggatcag gccgatcacg tgcgcggcgg gagcgagcgc
     1321 gaggtgggtc tcccccacga cgaggaccgg cagcgcgagc agcaggtcga aggtcgccgc
     1381 cttgcccacc cagacgacgt cgggcgggcg cacgccgcgg cttctgagca gggggaagcc
     1441 ggcgagcagg aggacgtccc gcccgacgag cagcgccacc gcgaggagcg gcaggacgcc
     1501 ccgcaccacc agggcgacgg ccaccgaggc gatcaggagg cggtcggcga gggggtcgag
     1561 gagctgcccg agcctcgaga cccggcccgt gcgcctggcg agccagccgt ccacccagtc
     1621 ggtgctgccg accaccgcgg tgagccacag cccgatcgcg tagtgcccgg tgccgagctg
     1681 ccacgcgcac accggcatga gcagcaggcg ggcggacgag atcgcgttcg gcgcggtcag
     1741 gatcgccgtc gacggcggct gggcggcatc cgccggcccg atcgcgtcca gggggccggg
     1801 cgcggccgcg acctgtgcct cgcccggccc ggcggccggg gcgtcggcgg cggggccgcg
     1861 gcgtgcgggc tccggcccgt tgggcttgct catgaaacga gcgtataccc gcccgggcca
     1921 caccgaccgg tgcccggcgg tcacaccggg atcggcacgt cgatcgaggt gacgagttgt
     1981 ggtcgggacg gccggatttg aaccggcgac ccccagtccc ccagactggt gcgctaacca
     2041 agctgcgcca cgtcccgagc gagggccctg gcagccgggc gcgccagacc ctgggcgggc
     2101 gcaaggatac cacgggccag gcggccgggc gccgccgacg gctcaggcgt cgtcccggcg
     2161 ctccctggtc cgcacccggc cgatcacccg caccggcgtc ccctggaagc cgaaggtgcg
     2221 gcgcaggccg tgctcgaggt agcggcggta ggcgggggcc agcacgccgg tggcgaagaa
     2281 gacgaactcg ggcggcgcca cccgcgcctg ggtgacgtag cggaccttga ccgccttgcc
//
LOCUS       DASPYY010000381         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1329731, whole genome shotgun sequence.
ACCESSION   DASPYY010000381 DASPYY010000000
VERSION     DASPYY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329731"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..>1510)
                     /locus_tag="VEP73_05910"
     CDS             complement(106..>1510)
                     /locus_tag="VEP73_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYT25998.1"
                     /translation="YCPDGHVTVDREDPLKCAECGATDLRQDDDVLDTWFSSGLWPLS
                     TLGWPDETEDLADFYPTSVLVTGYDIIFFWVARMLMFGVHFQPPAPFDVVAIHGMVRD
                     AQGKKMSKSFGNVIDPLELMDRYGTDALRFALIRGANPGADVPLAEEWVEGARNFANK
                     LWNLARFVLSAEPEGAEAPGEPGASRTQDGAALADRWLASRLERTRAAATAAYQAYDP
                     AEAARLLFAFAWSELADWAVELAKPRLAAGGEDARRAWAALAGALDATLRLLHPVMPF
                     VTEELGRALTGVSTITLGPWPAARPGEVDEAAEADMAALQEVIAAVRRFRAEHRVPSA
                     RRPRLTIVPASAAQAELLLAEAESVRRLARLESVEVAPADRSTGAPGPSAKVLAAGAE
                     LFLPLGGLLDLDEERDRLRRELATLGQERARAEGKLANPAFVSKAPVDVVGKARARLA
                     EVEEAVAKVQAQLAELG"
BASE COUNT          183 a          577 c          555 g          195 t
ORIGIN      
        1 agcgcgtcga ccgcctgctg gtactccatc cgctcggcac tcctccgggg tcgggacgtc
       61 cggccgtcgg tcgattgtat tgacggccgg gcgtcccgcg cggcctcagc cgagctcggc
      121 gagctgggcc tggaccttgg cgacggcctc ctcgacctcg gcgagccgcg ccctcgcctt
      181 gccgaccacg tcgacggggg ccttcgacac gaacgccggg ttggcgagct tcccctcggc
      241 ccgagcgcgc tcctgcccca gggtggccag ctccctgcgg agccggtccc gctcctcgtc
      301 gaggtcgagc agccccccga gcggcaggaa cagctcggcg ccggccgcga gcaccttggc
      361 gctaggaccg ggcgcccctg tgctccggtc cgcgggggcc acctccacgg actcgagccg
      421 cgcgagccgg cggacgctct cggcctcggc cagcaacagc tcggcctggg ccgcgcttgc
      481 cggcacgatc gtgaggcgcg gccggcgcgc cgacggcacc cggtgctcgg cgcggaagcg
      541 gcgcacggcg gcgatcacct cctgcagcgc ggccatgtcc gcctccgcgg cctcgtcgac
      601 ctcgccgggc cgcgccgccg gccagggacc gagcgtgatc gtcgagacgc cggtgagcgc
      661 gcggccgagc tcctcggtca cgaacggcat caccgggtgc agcaggcgca gggtggcgtc
      721 gagcgcgccg gccagggccg cccaggcgcg gcgcgcgtcc tcgccgccgg ccgcgagccg
      781 cggcttggcc agctccaccg cccagtcggc cagctcgctc cacgcgaacg cgaagagcag
      841 gcgcgcggcc tcggccgggt cgtaggcctg gtaggcggcg gtcgccgccg cccgggtccg
      901 ctcgagcctg gaggcgagcc accggtcggc gagggcggcc ccgtcctggg tgcggctcgc
      961 gcccggctcg ccgggcgcct cggcgccttc tggttccgcg gacagcacga agcgggcgag
     1021 gttccagagc ttgttggcga agttgcgggc gccctcgacc cactcctcgg cgagcggcac
     1081 gtccgcgccc gggttggccc ccctgatgag ggcgaagcgc agcgcgtcgg tgccgtagcg
     1141 gtccatcagc tcgagcgggt cgatcacgtt gccgaacgac ttcgacatct tcttgccctg
     1201 cgcgtcgcgc accatcccgt ggatggccac cacgtcgaag ggcgccgggg gctggaagtg
     1261 gacgccgaac atgagcatcc gggccaccca gaagaagatg atgtcgtacc cggtcaccag
     1321 caccgaggtc gggtagaagt cggccaggtc ctcggtctcg tccggccagc cgagcgtcga
     1381 gagcggccac agcccgctcg agaaccacgt gtcgagcacg tcgtcgtcct ggcggaggtc
     1441 ggtggcgccg cactcggcgc acttgagcgg gtcctcgcgg tccaccgtca cgtgcccgtc
     1501 cgggcagtac
//
LOCUS       DASPYY010000382         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1329927, whole genome shotgun sequence.
ACCESSION   DASPYY010000382 DASPYY010000000
VERSION     DASPYY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329927"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VEP73_05915"
     CDS             complement(<1..254)
                     /locus_tag="VEP73_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYT25999.1"
                     /translation="MTASARDVALAALGRVDGGAWANLVLPGLLARSGLGAGDRAAVT
                     DLVNGTLRLRGALDVQLAPLSRQPLARLEPLVRSGLRLGA"
     gene            complement(251..1273)
                     /gene="fmt"
                     /locus_tag="VEP73_05920"
     CDS             complement(251..1273)
                     /gene="fmt"
                     /locus_tag="VEP73_05920"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYT26000.1"
                     /translation="MRVVYFGTPDAAVPPLTALLASSHEVVGVVTRPDRPRDHRGGRP
                     SPSPVRRAAEAAGVPVVQPERGRDPALPGQLAAFRADVGFTCAFGFLLPPAVLAAFPR
                     GVLNLHFSLLPAYRGPAPVQRALLDGRTVTGVSTFVIDEGMDTGPLLLSAEVPILADE
                     DAGALTARLAEVGARLAVDTLDTLEAGAVSPRPQPGDGATLAPKVAPEEARLDWRAPA
                     SRVVNAVRAFTPAPGAWTTLRGRRLKVLSARAVAGPGAASPGLAPPATARDGAAGPPA
                     GAPGAAPALAPGQLGLGPGDALLAGTGDGQVELVLVQPEGRRAMPGADFARGARLGAG
                     EWLGDR"
     gene            complement(1276..>1558)
                     /locus_tag="VEP73_05925"
     CDS             complement(1276..>1558)
                     /locus_tag="VEP73_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYT26001.1"
                     /translation="LSLPGIYYDCKRAMEVTVAAQDVRGVPVVVEGAGLLARVFQHEI
                     DHLDGVLFLERLSRGDRKRAMKQWRERDLVLEAPGVAPHVHRSDPAKSL"
BASE COUNT          190 a          572 c          657 g          139 t
ORIGIN      
        1 tacgcgccga ggcgcaaccc gctgcgcacg agcggctcga ggcgcgcgag gggctgccgg
       61 gagagcggcg cgagctggac gtcgagcgcg ccgcgcagcc gcagcgtgcc gttcacgagg
      121 tcggtgaccg cggcccggtc gcccgcgcca aggcccgacc gggcgagcag gccgggcagg
      181 acgaggttcg cccaggcgcc cccgtcgacc cggccgagcg cggcaagggc gacgtcgcgg
      241 gccgaggcgg tcaccggtcc ccgagccact cccctgcccc gagccgagcg ccccgggcga
      301 agtcggcacc tggcatcgcc cgcctgccct cgggctggac gaggacgagc tcgacctggc
      361 cgtcgccggt gccggcgagc agggcgtccc cggggccgag gccgagctgc ccaggggcga
      421 gcgctggcgc ggcccccggc gcgcccgcgg gcgggccggc cgccccgtcc cgcgccgtgg
      481 cgggcggggc caggccgggc gatgccgcgc cgggaccggc gaccgctctg gcgctgagga
      541 ccttgagccg ccgcccgcgc agggtggtcc aggcgcctgg ggcgggcgtg aaggcgcgca
      601 ccgcgttgac gacgcgcgag gccggcgccc gccagtcgag gcgggcctcc tccggggcga
      661 ccttcggggc cagggtcgcg ccgtcgccgg gctgcgggcg gggcgagacc gcccctgcct
      721 cgagcgtgtc gagggtgtcg accgcgagcc gggcgcccac ctcggccagg cgcgcggtca
      781 gcgcgccggc gtcctcgtcg gcaaggatcg gcacctcagc cgacaggagc agcgggccgg
      841 tgtccatccc ctcgtcgatg acgaacgtgg agaccccggt gacggtgcgc ccgtcgagca
      901 gggcgcgctg caccggggcg ggcccgcggt aggcgggcag cagcgagaag tgcaggttga
      961 gcacgccccg ggggaacgcg gcgagcacgg cgggcggcag caggaagccg aacgcgcagg
     1021 tgaagccgac gtcggcgcgg aacgcggcga gctggcccgg cagggcgggg tcgcggccgc
     1081 gctcgggctg gacgaccggc acgccggccg cctcggcggc ccgcctgacc ggggaggggg
     1141 acggccggcc gccgcggtgg tcgcggggcc ggtcgggccg ggtgacgacc ccgacgacct
     1201 cgtgcgagga ggcgagcagg gcggtgagcg gcgggacggc cgcgtccggg gtgccgaagt
     1261 agacgacgcg cacgcctaca gcgacttggc cgggtcggag cggtggacgt gcggcgcgac
     1321 cccgggcgcc tcgagcacga ggtcgcgctc gcgccactgc ttcatggccc gcttgcggtc
     1381 gcccctggag aggcgctcca ggaacagcac cccgtccagg tggtcgatct cgtgctggaa
     1441 gacgcgtgcg agcaggccgg cgccctcgac gacgaccggc acgccgcgga cgtcctgggc
     1501 ggcgacggtc acctccatcg cccgcttgca gtcgtagtag atgccgggga gcgacagg
//
LOCUS       DASPYY010000383         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_346343, whole genome shotgun sequence.
ACCESSION   DASPYY010000383 DASPYY010000000
VERSION     DASPYY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346343"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEP73_05930"
     CDS             complement(<1..312)
                     /locus_tag="VEP73_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26002.1"
                     /translation="MHLPTRKSLAPLAVAAALGLAVLPAWGANAAGTTAGTTVAAAAD
                     TTVDTVVDTTVDTVVDTTVATDPPTTVVATDPATTVTTDPPTTAPPATDPPTTAPPAT
                     DP"
     gene            complement(467..>1879)
                     /locus_tag="VEP73_05935"
     CDS             complement(467..>1879)
                     /locus_tag="VEP73_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HYT26003.1"
                     /translation="PAEVVREVEELFLDLDADEDQIDFPILYTNARAGRAGPRPDELA
                     GDLGPLFEAIVATTPPPVYEPGHPLQFLVTNLDADPYVGRLAVGRVWQGEVRLGDRVV
                     VAKGDGTLSPAKVTKVLGATGLSRVEVDGAGPGDIVAIAGLGDVTVGETVTDPDDPRP
                     LPLATIDEPSLSMQFGVNTSPFAGNEGTFLTSRHLKERLDREVLGNVSIRVLPTESPD
                     MFEVQGRGELQLAVLVEQMRREGYEVQVSKPEVITRLVDGRVHEPYEAVTLDVPEEFV
                     GVVTQVLSLRRGRMTNMVNHGTGWVRLEFRVPARGLVGFRSTLLTDTKGTAILHHLFD
                     GYDEWAGEIHHRLTGVLVADRTGTTTGYALDQLQERGELFVGPGTEVYEGMIVGENAR
                     TEDIDVNPTREKQKTNMRTHAADEAIRLVPPRQLSLEQALEFVSVDELVEVTPASTRL
                     RKRVLDAQARGRAAKRARKG"
BASE COUNT          238 a          658 c          709 g          274 t
ORIGIN      
        1 ggggtcggtg gccggcggcg cggtggtggg ggggtcggtg gccggcggcg ccgtggtcgg
       61 cgggtcggtc gtcacggtcg tggccgggtc ggtcgcgacc acggtggtcg gcgggtccgt
      121 ggcgacggtc gtgtcgacga ccgtgtcgac ggtcgtgtcg accaccgtgt cgacggtcgt
      181 gtccgcggcc gcggcgacgg tcgtgccagc ggtcgtgccg gcggcattcg cgccccacgc
      241 cggcagaacg gccaggccga gggcggcagc cactgcgaga ggggcgagcg acttcctggt
      301 gggtaggtgc acgcggcgct cctcgccggt tggccggccg gaatgggcgg cagcgcccga
      361 caccgggacg aatccgcaac ggtataacgg agccggcaat cggagtcaac ggcaccagtg
      421 catcgaacgc gcaacgatgt acccgcggcg gcatgcggcg cgggcgtcag cccttcctgg
      481 cgcgcttcgc cgcccgccca cgggcctgcg cgtccagcac ccgcttgcgc agccgggtcg
      541 acgccggcgt gacctcgacc agctcgtcga cgctgacgaa ctcgagggcc tgctcgagcg
      601 agagctggcg cggcggcacg agccggatcg cctcgtcggc ggcatgcgtg cgcatgttcg
      661 tcttctgctt ctcgcgggtc gggttcacgt cgatgtcctc ggtgcgggcg ttctcaccga
      721 cgatcatgcc ctcgtagacc tcggtgcccg ggccgacgaa cagctcgccc cgctcctgga
      781 gctggtcgag cgcgtagccg gtggtggtgc ccgtccggtc ggcgacgagc accccggtga
      841 gccggtggtg gatctcgccg gcccactcgt cgtagccgtc gaacaggtgg tggaggatcg
      901 ccgtgccctt cgtgtcggtg agcaaggtcg agcggaagcc gacgaggccg cgggccggga
      961 cccggaactc gagccggacc cagccggtgc cgtggttcac catgttcgtc atgcgccccc
     1021 tgcgcaggga gagcacctgg gtgaccactc ccacgaactc ctcgggcacg tccagcgtga
     1081 ccgcctcgta cggctcgtgc acgcggccgt cgaccagcct cgtgatgacc tcgggcttcg
     1141 acacctggac ctcgtagccc tcccgccgca tctgctcgac cagcaccgcg agctgcagct
     1201 cgccccggcc ctggacctcg aacatgtcgg gggactcggt cggaaggacc cggatcgaga
     1261 cgttgccgag gacttcgcgg tcgagccgct ccttcaagtg cctgctggtc aggaaggtgc
     1321 cctcgttgcc ggcgaacggg gaggtgttga cgccgaactg catcgacagg ctgggctcgt
     1381 cgatcgtcgc cagcggcagc ggccgcgggt cgtcggggtc ggtgaccgtc tcgcccacgg
     1441 tgacgtcgcc gagcccggcg atggccacga tgtcgcccgg cccggccccg tcgacctcga
     1501 cgcgcgacag gccggtggcc ccgaggacct tggtgacctt ggccggcgac agggtcccgt
     1561 cgcccttggc cacgaccacg cggtcgccga gccggacctc gccctgccag acgcgcccga
     1621 ccgcgagccg gcccacgtac gggtcggcgt cgaggttggt gaccaggaac tgcagcgggt
     1681 ggccgggctc gtagaccggc ggcggggtgg tcgcgacgat cgcctcgaac agcggcccga
     1741 ggtcgccggc gagctcgtcg ggccgcggcc cggcccgccc ggctcgcgcg ttggtgtaca
     1801 ggatcgggaa gtcgatctgg tcctcgtcgg cgtcgaggtc gagaaacagc tcctcgacct
     1861 cgcggaccac ctcggcggg
//
LOCUS       DASPYY010000384         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1049098, whole genome shotgun sequence.
ACCESSION   DASPYY010000384 DASPYY010000000
VERSION     DASPYY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049098"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VEP73_05940"
     CDS             complement(<1..967)
                     /locus_tag="VEP73_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYT26004.1"
                     /translation="MSKRDRNRARLAAAVGGAAVAAGVAAAALARRPARQGYRQSLFW
                     RLYDGTAQAVDRTVGWDRLPTPLGLMVLLGLRNLLRKHNLYDTTGLPAVDTPPVAPFD
                     PGLETARAADGTHNDLEHPAMAMAGSRFGRNVPLEHTFPEPLPALLEPSPREVSRVLL
                     TRPGGRIQAAEAANALVAAWLQFMIRDWFSHGRSVTDDPWQIQLLDDDPWPQRPMRIM
                     RTRPDPTRPAGTAGLPPTYVNTETHWWDGSQLYGSSKELQQLLRAGEGGKLRIGPDGL
                     PPRPTDPDRDPTRVPGFWLGLALMQHLFALEHNAICDRLRADYPSW"
     gene            complement(967..2208)
                     /locus_tag="VEP73_05945"
     CDS             complement(967..2208)
                     /locus_tag="VEP73_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HYT26005.1"
                     /translation="MARAPGTGPRSRPPRPAGTLSRLVNDLAVGLIRAERRIDPRFRP
                     AFDRWLRPPAAAAVQALVNLRRRDPRLGPAAERELPGEAAATAEIIAEMSAFTREHYA
                     PGAALRAGNTKTYGVVRGELAVRRDVPERLRHGIFAAPRTYRAWVRFAGPGPLAPPDL
                     DDNGVLSIGIKLMGVEGPKLLDHERWTQDLTGISAPTFTTPDVVENAKLQRRVREGVP
                     VWYFLDPFDSHLLDGIMQGLWSKTHANPLEAQYYSCAAYLLGEGQAMQYSVRPRAAPT
                     SRVPRRPGPDYLRAAMAATLARQAVELDLLVQLQTDPFRMPVENASVRWPERRSPFVP
                     VATLRLPSQRFDSPAQLALADSLSYNPWHCIPAHRPLGNQNRARKAIYLELSALRQTM
                     NGHPHVEPTGDEVFDQAEEVP"
     gene            complement(2208..>3119)
                     /locus_tag="VEP73_05950"
     CDS             complement(2208..>3119)
                     /locus_tag="VEP73_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HYT26006.1"
                     /translation="ISQPLVEGLPRTGPPAQTVRAGEIVLGYPNEYALYTDRPLLPAA
                     EDPGGLLPRDAGGSGDADLGRNGSYLVVRQLAQDVRGFRRFLERATTRADGGSDERDR
                     EWLAAKMVGRWPGGAPLVLSPDRDDPALADANDFAYAASDPDGLRCPLGAHVRRAHPR
                     DSLDPGPGSQKSIDVDKRHRILRRGREYGPPLPPDAPLDGPGTAPGADEERGLHFMCL
                     NANIARQFEFIQHTWVNNPKFDGLYDDPDPVVAASAALGGSFTVPARPVRRRVTGVPA
                     FVTVRGGGYFFLPGIRAIRYLASLGGT"
BASE COUNT          366 a         1091 c         1247 g          415 t
ORIGIN      
        1 accaggacgg gtagtccgcg cggagccggt cgcagatggc gttgtgctcg agcgcgaaca
       61 ggtgctgcat cagcgcgagc ccgagccaga agccggggac cctcgtcggg tcgcggtcgg
      121 ggtcggtggg gcggggcggc agcccgtccg gcccgatgcg cagcttgccg ccctcgccgg
      181 cgcgcagcag ctgctgcagc tccttgctgc tgccgtagag ctgggagccg tcccaccagt
      241 gggtctcggt gttgacgtag gtgggtggca ggccggccgt gccggcggga cgggtcgggt
      301 ccggccgggt ccgcatgatc cgcatgggcc gctgcggcca cgggtcgtcg tcgaggagct
      361 ggatctgcca ggggtcgtcg gtgacgctcc tcccgtggct gaaccagtcg cggatcatga
      421 actgcagcca cgccgccacc agggcgttgg ccgcctcggc ggcctggatg cggccgccgg
      481 gccgggtgag caggacccgg ctgacctcgc gggggctcgg ctcgagcagg gcgggcagcg
      541 gctcggggaa ggtgtgctcg aggggcacgt tgcggccgaa gcgggacccg gccatcgcca
      601 tggccgggtg ctcgaggtcg ttgtgggtgc cgtcggcggc gcgcgcggtc tccaggccgg
      661 ggtcgaacgg cgcgaccggg ggcgtgtcga ccgccggcag gccggtggtg tcgtagaggt
      721 tgtgcttgcg cagcaggttc cgcaggccca ggagcaccat caggccgagc ggcgtgggca
      781 gccggtccca cccgacggtg cggtcgaccg cctgggcggt gccgtcgtac aggcgccaga
      841 acagcgactg gcggtagccc tggcgggccg gccgccgcgc cagggcggcg gccgcgacgc
      901 ccgcggcgac ggccgcgccg cccaccgccg ccgcgagacg cgcccggttc ctgtcgcgct
      961 tgctcatcac ggaacctcct cagcctggtc gaagacctcg tcgccggtcg gctcgacgtg
     1021 cggatggccg ttcatggtct ggcgcagcgc cgacagctcg aggtagatgg ccttgcgtgc
     1081 ccggttctgg ttgccgagcg gacggtgcgc gggaatgcag tgccacgggt tgtacgagag
     1141 gctgtcggcg agggcgagct gcgcggggga gtcgaaccgc tggctgggca gccgcagcgt
     1201 tgccacgggc acgaacggcg agcggcgctc gggccatcgc accgaggcgt tctcgaccgg
     1261 catgcggaac gggtcggtct ggagctggac gagcaggtcg agctcgaccg cctggcgggc
     1321 gagcgtcgcc gccatcgccg cgcgcaggta gtcgggtccg gggcgccgtg ggaccctgct
     1381 cgtcggtgcc gcgcgcggcc gcaccgagta ctgcatcgcc tgcccctcgc cgagcaggta
     1441 cgccgcgcag ctgtagtact gcgcctccag cgggttggcg tgcgtcttgg accacagccc
     1501 ctgcatgatc ccgtcgagca ggtgggagtc gaacgggtcg aggaagtacc agacagggac
     1561 gccctctcgc acccgccgct gcagcttggc gttctcgacg acgtcgggcg tggtgaaggt
     1621 gggcgcgctg atgccggtga ggtcctgcgt ccaccgctcg tggtcgagca gcttcggccc
     1681 ctccacgccc atgagcttga tcccgatgct gagcacgccg ttgtcgtcga gatccggcgg
     1741 tgcgagcggt cccgggccgg cgaagcgcac ccaggcacgg taggtcctgg gtgccgcgaa
     1801 gatcccgtgc cgcagccgct ccggcacgtc gcgccgcacc gcgagctcgc cccggaccac
     1861 gccgtaggtc ttggtgttgc cggcccgcaa cgcggcgccg ggtgcgtagt gctccctggt
     1921 gaacgccgac atctccgcga tgatctcggc ggtcgccgcc gcctcgcccg gcagctcgcg
     1981 ctccgcggcg ggaccgagcc gcgggtcgcg gcgacggagg ttgacgagcg cctggacggc
     2041 cgcggcggcc ggcggccgca gccaccggtc gaaggccggc cggaagcgcg ggtcgatgcg
     2101 gcgctcggcc cggatgagcc cgaccgccag gtcgttcacc aggcgcgaga gcgttccggc
     2161 cgggcggggc ggccgcgacc ggggcccggt gcccggggcg cgcgccatca cgtgcctccc
     2221 aggctcgcca ggtagcggat ggcccggatg cccggcagga agaagtagcc gccgccccgg
     2281 acggtgacga acgccggcac gcccgtcacc cggcgccgca ccggccgggc cggcacggtg
     2341 aagctgccgc cgagcgccgc ggaggccgcc acgaccgggt ccgggtcgtc gtacaggccg
     2401 tcgaacttgg ggttgttcac ccaggtgtgc tggatgaact cgaactgcct ggcgatgttg
     2461 gcgttcaggc acatgaagtg caggccgcgc tcctcgtcgg ctcccggggc ggtcccgggg
     2521 ccgtcgagcg gcgcgtccgg cggcagcggg gggccgtact cccggccgcg gcggaggatc
     2581 cggtgccgct tgtccacgtc gatcgacttc tgggagcccg gtccggggtc gagcgagtcg
     2641 cggggatggg cgcgccggac gtgcgcgccg agggggcagc gcagcccgtc ggggtcgctc
     2701 gcggcgtagg cgaagtcgtt cgcgtccgcg agcgccgggt cgtcccggtc gggcgagagc
     2761 accagcgggg ccccgcccgg ccagcgcccg accatcttgg cggcgagcca ttcgcggtcc
     2821 cgctcgtcgc tgcccccgtc tgcccgcgtc gtcgcccgct cgaggaaccg ccggaagccg
     2881 cgcacgtcct gggcgagctg ccggacgacc aggtagctgc cgttgcggcc gaggtcggcg
     2941 tcgcccgacc cgcccgcgtc gcggggcagc aggccgcccg ggtcctcggc ggccgggagc
     3001 agcggccggt cggtgtacag ggcgtactcg ttggggtagc cgaggacgat ctcgccggcc
     3061 ctgaccgtct gcgccggcgg gccggtcctg gggagcccct cgaccagcgg ctgcgagat
//
LOCUS       DASPYY010000385         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1751686, whole genome shotgun sequence.
ACCESSION   DASPYY010000385 DASPYY010000000
VERSION     DASPYY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751686"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..2175
                     /locus_tag="VEP73_05955"
     CDS             529..2175
                     /locus_tag="VEP73_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYT26007.1"
                     /translation="MENHSFDNLWGRWPGVQGLAGDPAGTPRNTQVDRAGRPFACLLQ
                     NDVNLTTSADPPPNGGLTPSCSTKPPAAYNSHFPNQPFEIDQYLTPGSTTCYHATPTA
                     PFGPANGVPDGTGDPGGCTRDLVHRFYQEPYQIDGGKMDRYTAGSDAVGLTQGYYDTT
                     ELPLYQYLTQYLTGDPGAPRYAIADRFHQAAFGGSFLNHQWLVAARTPQWEGGAVHDG
                     GGGDLHSIVGADGHPNNTYPLHSGPVYPAPGGVKDAALTENADPATGACAPPAGSPTP
                     PPGTVCGDWAVNTIQPAFQPYQPGTAPARRLPALHKATIGDELTAKGISWNWYAGGWD
                     NAAGVTDGPGWTNGAGPTCADPGTLATATYPYCPNVLFQFHHQPFNYFANYGPGQPGR
                     AHLKDEQDFLQNLKAGELPAVSFVKPVGEDNEHPGYTSVTAGERHLVDLIKAIKADEA
                     DWPSTAVVVTYDEFGGAWDHVKPPTAPGVSDVWGPGTRIPAMVISPQLPEQAAVDHIQ
                     YDTVSILAMLERRFGLAPLSPRDAAVHDLGRIFREGLDQL"
     gene            2336..>2988
                     /locus_tag="VEP73_05960"
     CDS             2336..>2988
                     /locus_tag="VEP73_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HYT26008.1"
                     /translation="MLICAFAASDTGMVRDGNEDSLYRGTTLFAVADGLGGHSGGEVA
                     SAVAVGEVARLDGTRPASAGAAREALVGAFAAANRVVLEAAEADPALHGMGTTLTAAL
                     LHGEGLSIAHVGDSRAYLLRRGAALQQLTSDQTVVAEMVRQGRLSLESAARHPYRSVV
                     TAAIGLEHDVEVEVVGPLALGAGDQVLLCSDGLTEPVGDQTIAEVLGAATDGDAACRA
                     "
BASE COUNT          436 a         1140 c         1064 g          348 t
ORIGIN      
        1 acggcggtcg tgaacgaccc gttctggtgg gaggcggacg agaagttctt cgagtgcacc
       61 ctcgcccgca agctcggcgt ggcggtgccg aggaccgtcg cgctgcccaa caaggagtac
      121 gtcggctaca tcgaccccga cgagcaggcc gaccaccttc gacatgcgag ctgctctcgc
      181 catgcgagct cctctcgacg ccccgctcgg ggtcgcggac gaccggccgg gcggcgccgg
      241 gggccgcccg cgctcggtct ggtacccagc ctaaccagcg gaccggcacg cgggaaggcc
      301 gtcgtccaca gccccctcgc ttcgcaccgc cccatgtgtt acgaatgccg gacgtcgccg
      361 gacccggggg tgtggaatga gaaggatgct gctggccgtg atcggtgcgt cgctcgtgtg
      421 cgcgtcggtg gcgacgacct cgtcggtcgc ggcgccggcg cgcgcgggcg ggagcccgct
      481 cgccggcatc gagcacgtgg tcgtcatcta catggagaac cactcgttat ggagaaccac
      541 tcgttcgaca acctgtgggg ccggtggccc ggcgtccagg ggctcgcggg cgacccggcg
      601 gggacgccgc ggaacacgca ggtcgaccgg gcgggcaggc cgttcgcctg cctgctccag
      661 aacgacgtga acctgaccac ctcggccgac ccgccgccca acggcggcct cacgccgagc
      721 tgcagcacca agccgcccgc ggcctacaac agccacttcc ccaaccagcc gttcgagatc
      781 gaccagtacc tcacgcccgg gtcgacgacc tgctaccacg ccaccccgac cgcgccgttc
      841 gggccggcca acggcgtgcc cgacggcacc ggcgacccgg gcggctgcac ccgcgacctg
      901 gtgcaccgct tctaccagga gccgtaccag atcgacggcg gcaagatgga ccgctacacg
      961 gccggcagcg acgccgtcgg cctgacccag ggctactacg acaccaccga gctgccgctg
     1021 taccagtacc tcacccagta cctcaccggc gacccgggcg cgccccgcta cgccatcgcc
     1081 gaccgcttcc accaggccgc cttcggcggg tcgttcctga accaccagtg gctcgtcgcc
     1141 gcccggacgc cgcagtggga gggcggcgcc gtccacgacg gcggcggcgg cgacctccac
     1201 tcgatcgtgg gcgccgacgg ccacccgaac aacacctacc cgctgcactc cgggccggtc
     1261 tacccggcgc cgggcggggt gaaggacgcg gcgctgaccg agaacgccga cccggcgacc
     1321 ggggcctgcg cccccccggc tggctccccc acccctccgc cgggcaccgt ctgcggcgac
     1381 tgggcggtca acaccatcca gcccgccttc cagccctacc agccgggcac ggccccggcg
     1441 aggcggctcc cggcgctgca caaggcgacc atcggcgacg agctgaccgc gaaggggatc
     1501 agctggaact ggtacgcggg cggctgggac aatgccgccg gcgtgaccga cggcccgggc
     1561 tggaccaacg gtgccggccc cacctgcgcc gacccgggca ccctggccac ggcgacctac
     1621 ccgtactgcc cgaacgtgct gttccagttc caccaccagc cgttcaacta cttcgcgaac
     1681 tacggccccg gccagcccgg gcgcgcgcac ctcaaggacg agcaggactt cctgcagaac
     1741 ctgaaggccg gcgagctgcc cgcggtgtcg ttcgtgaagc cggtgggcga agacaacgag
     1801 caccccggct acaccagcgt gaccgccggc gagcggcacc tggtcgacct catcaaggcg
     1861 atcaaggccg acgaggccga ctggccgtcc accgcggtcg tcgtcaccta cgacgagttc
     1921 ggcggcgcgt gggaccacgt caagccgccg accgcgcccg gggtctcgga cgtgtgggga
     1981 ccggggaccc gcatcccggc catggtgatc tcgccgcagc tgccggagca ggccgcggtg
     2041 gaccacatcc agtacgacac cgtgtcgatc ctcgcgatgc tcgagcgccg cttcggcctc
     2101 gcgccgctca gcccgcgcga cgccgcggtc cacgacctcg ggcggatctt ccgggagggg
     2161 ctggaccagc tgtgacgcag gcggtcccgg ccggcggccc gtggcggccc cggccgggac
     2221 cggaggggcg gggtgccggg gcgctgcggg cactatgatc tggggcagca gccagcgcga
     2281 ccaaggcatc gggagccggc gccatcgggg agccggcggg accaaggcat cgggcatgct
     2341 catctgcgca ttcgccgcct cggacacggg aatggtccgg gacggcaacg aggacagcct
     2401 ctaccggggc acgacgctgt tcgcggtcgc ggacggcctc ggcggccact ccggcggcga
     2461 ggtcgcctcg gcggtcgcgg tcggcgaggt cgcccggctc gacgggacga ggccggccag
     2521 cgccggagcg gcccgcgagg cgctggtggg cgcgttcgcg gccgcgaacc gggtcgtgct
     2581 cgaggccgcc gaggccgacc ccgcgctgca cgggatgggc acgacgctga ccgccgcgct
     2641 gctgcacggc gagggcctca gcatcgccca cgtcggtgac agccgtgcct acctgctgcg
     2701 ccgcggcgcg gcgctgcagc agctcaccag cgaccagacg gtcgtggccg agatggtccg
     2761 ccagggccgg ctctcgctgg agagtgccgc ccgccacccg taccggagcg tggtgaccgc
     2821 cgcgatcggg ctcgagcacg acgtcgaggt cgaagtcgtg gggccgctcg cgctcggggc
     2881 cggcgaccag gtcctgctgt gctccgacgg gttgaccgag ccggtcggcg accagacgat
     2941 cgccgaggtg ctcggcgcgg ccaccgacgg cgacgcggcc tgccgggc
//
LOCUS       DASPYY010000386         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_768243, whole genome shotgun sequence.
ACCESSION   DASPYY010000386 DASPYY010000000
VERSION     DASPYY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768243"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VEP73_05965"
     CDS             <1..774
                     /locus_tag="VEP73_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26009.1"
                     /translation="RLLRPVVPAGEPLVPAGEGKGASWPAPAGGALAELRVTVVDPDP
                     AKVGRRFSGACVELGLASYPGFALTAPPGRETAFAVYWPALVPSELVPHRVVLPDGRT
                     VGVPPAPGAAPVDLADPLAPPRPPASDVPGGPVRQAPLAAVAGARSGDKGGNANLGVW
                     ARSREGYAWLERLLTVERLRELLPEAAGLDVRRYELPNLLALNFLVRGLLGEGAAAST
                     RPDPQAKGLGEQLRACLVDVPVALLDRDAPRRAAPGPAG"
     gene            complement(811..1089)
                     /locus_tag="VEP73_05970"
     CDS             complement(811..1089)
                     /locus_tag="VEP73_05970"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26010.1"
                     /translation="MPAGSVVVFDGYLLHRSLPNTGRHGLRRALVSHYMSAESLLPWF
                     LPPAGVTPGRADHRDVVLVAGSDPYAYKGTVDVMRPHVRPDGQGGCLR"
     gene            complement(984..1718)
                     /locus_tag="VEP73_05975"
     CDS             complement(984..1718)
                     /locus_tag="VEP73_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYT26011.1"
                     /translation="MTDSSVQATTAGATRHQTGSGSSGLVSRYRRDGYVVLADALSAG
                     AVQALRDEAVRICRGELGAVDGVQRAGADEPDELVVRRYLCIHFPHKLSERMRGALAH
                     PVVVDALQQLIGPNVKAMQSMLFIKSEGRPGQAWHQDELFIPTRDRSLTAAWIALDDA
                     TVENGCLWVLPGSQRRGVRTRTGTTTTPGSTAKSRRTTSPTSTTARSPSRCPRVPSSC
                     LTAICCTGRCRTPGVMACAARWSATT"
     gene            complement(1886..2116)
                     /locus_tag="VEP73_05980"
     CDS             complement(1886..2116)
                     /locus_tag="VEP73_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HYT26012.1"
                     /translation="MDLAEVRALASGGVHYLASDTTGVVCFPTCPNARRISAAHRHGF
                     RTVGQAARAGYRPCRHCRPAPAAASAEGARKP"
     gene            complement(2182..2703)
                     /locus_tag="VEP73_05985"
     CDS             complement(2182..2703)
                     /locus_tag="VEP73_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26013.1"
                     /translation="MADGAEEVTAILARLADDLDAGFVDLFRAYQRIVYSTALGVCRR
                     PADAEDVTAEAFLHAYRALRGYDRRRIAALQPRAWLVTIMLNAWRNTGRTTLRRPRQV
                     PLDEAVDPPDRSDSVERIVERHETGRELAALLARLPEAQRVAVVLRHVGGLSIAEVAA
                     AADADPLPPGGPL"
     gene            complement(2920..3819)
                     /gene="arsM"
                     /locus_tag="VEP73_05990"
     CDS             complement(2920..3819)
                     /gene="arsM"
                     /locus_tag="VEP73_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HYT26014.1"
                     /translation="MTTSPADTGQPTPDELREQVRARYAAAATRVSTGQGGRGQPADC
                     GCGQPVDCGCGSGCCTSAEEPGFGGELYTTGDRDALPETAVLASLGCGNPTAVAELRE
                     GETVLDLGSGGGIDVLLSARRVGPTGKAYGLDMTEEMLALARANAEQADAANVEFLKG
                     QIEAIPLPAGTVDVVISNCVVNLSVDKPAVFAEVFRVLRPGGRLGISDVVAEDHLTPD
                     DRAERGSYVGCIAGTLAATEYEQGLAAAGFAGVSVTFTHQVGDGLHGAIVKATKPGGA
                     APAAQLPAAGRATLPVTSAQGCC"
     gene            complement(3816..4232)
                     /locus_tag="VEP73_05995"
     CDS             complement(3816..4232)
                     /locus_tag="VEP73_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT26015.1"
                     /translation="MRDQLPDPPAEPTATATRGGASGSAAACCAPLAATSLTDAEARA
                     TADLFKALGDRHRVRIVNLLATSPDPVCVCDLTPALGLSQPTVSHHLKKLVLAGLLRR
                     EQRGTWAYYSLDQNTLRRLAAVVDPDGATAREGATP"
     gene            4287..5003
                     /locus_tag="VEP73_06000"
     CDS             4287..5003
                     /locus_tag="VEP73_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYT26016.1"
                     /translation="MIIRVCIDVCQSISADQGRNLAGVASSADAVNTYYMRDSRSTLL
                     AAAAEEFARHGPKGARIQAIVERAGVNERMIYHHFGSKDGLYAAVLEAQAHGLVQAWL
                     QIIDTAAEMAPYEGMRAVLVGFADTFAARPLLVALTLHEALGGWRTLPLPTADMAPFP
                     ELRGLYERGQREGVFRSDCPFEIAHGVAMSALVGSAVLAPRGAEVLKAAGLDADPARL
                     RDQVVGQLLDGMTGPPKRTG"
     gene            5083..5610
                     /locus_tag="VEP73_06005"
     CDS             5083..5610
                     /locus_tag="VEP73_06005"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HYT26017.1"
                     /translation="MTTLDLSPASAAPGAVKTRALLVCGAVAGPLFTVAWVVEGATRA
                     HYNPLRHPVSSLELGDFGWTQRANFIAAGGLTLAFASGLRRARRPLGGSTWGPLLVGA
                     HAIGLLGAGIFVTDPVSGYPPGTPTSSRTTAACTPSSTTCSRSVPSWDCPSPASCSPG
                     ASPDGVSADGRSTRP"
     gene            5526..5795
                     /locus_tag="VEP73_06010"
     CDS             5526..5795
                     /locus_tag="VEP73_06010"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HYT26018.1"
                     /translation="MGLPVACFVFARRFAGWGERGWAIYSAVTGVVFTVAFVLASMAF
                     GQAERLVALGGLFQRITITVGWGWLTLLAVHLLRDLSQRPGRMRT"
     gene            5959..>6553
                     /locus_tag="VEP73_06015"
     CDS             5959..>6553
                     /locus_tag="VEP73_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26019.1"
                     /translation="MRQLTVLLGVFAVVAGVLAAPAAADVTRSTVHGTSAYAMFEVPG
                     GTLVISAFDANAYQDGTGVSHAAFVYAELFSLECTPDGLTCTGKDWLASATLDDGDAV
                     RIAGDLWTASLGPLVVDARKLTCIDTYEPGGDGFPVSTSCTQVTEPTTLSGSWAATGQ
                     IADYHDSRVTDQDGVHTVFTAAGVSRPATATAEAFGTS"
BASE COUNT          838 a         2361 c         2420 g          934 t
ORIGIN      
        1 aggctgctcc ggcccgtcgt ccctgccggc gaacccctcg tccccgccgg cgaaggcaag
       61 ggcgcctcct ggccggcgcc cgccggcggc gcgctggccg agctccgcgt caccgtggtc
      121 gaccccgacc cggcaaaggt cggccggcgc ttctcgggcg cctgcgtcga gctcggcctg
      181 gcgtcgtacc cgggcttcgc cctgaccgcg ccgcccggcc gggagaccgc gttcgcggtg
      241 tactggccgg cgctcgtgcc gagcgagttg gtcccgcacc gggtcgtcct ccccgacgga
      301 cgcaccgtcg gcgtcccgcc cgcgccaggc gccgcaccgg tcgacctcgc cgacccgctc
      361 gcgccgccgc gcccgcccgc gtcggacgtg cccggcgggc cggtacgcca ggcgccgctc
      421 gccgcggtgg ccggcgcgcg ctcgggcgac aagggcggca acgccaacct cggcgtctgg
      481 gcccgcagcc gggagggcta cgcctggctc gaacggctgc tgaccgtcga gcggctccgc
      541 gagctgctgc ccgaggcggc cggcctcgac gtgcggcgct acgagctgcc caacctgctc
      601 gcgctgaact tcctcgtccg cggcctgctc ggggagggcg ccgccgcgtc gacccgcccg
      661 gacccgcagg ccaagggcct cggtgagcag ctcagggcct gcctcgtcga cgtcccggtc
      721 gccctgctcg accgtgacgc cccgcggcgg gccgccccgg gacccgcggg ctgatcgagc
      781 cgggcccttt ctgccggacc ggactcctgg tcagcggagg cagccgccct gcccgtcggg
      841 tcggacgtgc ggccgcatca cgtcaaccgt gcctttgtag gcgtagggat cgctaccggc
      901 gaccagcacg acgtcgcggt ggtcggctcg gcccggcgtg acacccgcgg gcggaaggaa
      961 ccagggcaac agcgattccg cgctcatgta gtggctgacc agcgcgcggc gcaggccatg
     1021 acgcccggtg ttcggcagcg acctgtgcag cagatagccg tcaaacacga cgacggaacc
     1081 cgcgggcacc tcgacggcga ccgcgccgtc gtcgaggtag gggaagtcgt acgcctcgac
     1141 ttcgcagtcg aaccgggggt cgtggtggtc ccggtccggg tacggacacc acgtcgctgc
     1201 gagccgggaa ggacccacag gcagccgttc tcgacggtcg cgtcgtccag ggcgatccac
     1261 gccgcggtca gcgaccggtc tctggtcggg atgaacagct cgtcctggtg ccacgcctgc
     1321 cccggcctgc cctccgactt gatgaacagc atcgactgca tggccttgac gttggggccg
     1381 atgagctgtt gcaaggcgtc gacgacgacc gggtgggcca gcgccccgcg catgcgttcg
     1441 gaaagcttgt gcgggaagtg gatgcacagg tagcggcgga cgacgagctc gtcgggctca
     1501 tcggccccgg cccgctggac gccgtcgacg gcgcccagct ctcctcggca gatgcgcacg
     1561 gcctcgtccc gcagggcctg caccgcaccg gccgacagcg catcagccag gacgacatag
     1621 ccgtcgcgcc ggtagcggct gaccagcccg gagctaccgg agccggtctg atggcgggtg
     1681 gccccagcgg tggtcgcctg cacggagctg tcggtcatgc cgccagtctg ggggacggcg
     1741 ccgtcgttga gaatgtccag cagccgcgag aacctatccg actcaacctc ggcgaggacc
     1801 gctcccgcaa ccagcagcag accggctgcg gctgctgctc gggctgtgac gggcgacgcc
     1861 gcctcctggg acgtccggcc cgaggctacg gcttccgtgc tccctctgcc gaggccgcgg
     1921 ccggcgcggg acggcagtgc cggcacggcc ggtagccggc ccgcgccgcc tggccgaccg
     1981 tgcggaagcc gtgccggtgc gcggcgctga tccggcgcgc gttcgggcag gtcgggaagc
     2041 agaccacgcc ggtggtgtcg ctggccaggt agtggacccc gccgctggcg agcgcgcgga
     2101 cctcggcgag gtccacgccc tcgacgccga gcagcgcctc ctttggccgg cgcgccgaac
     2161 acgtagtcgc cgggcgtgcc gtcagagcgg accacccggt ggcaggggat cagcatcggc
     2221 agcggccgcg acctcggcga tggagagccc gccgacgtgc cgcagcacca ccgcgacacg
     2281 ctgggcctcc ggcaggcggg cgagcagcgc ggcgagctcg cggccggtct cgtgccgctc
     2341 cacgatgcgc tcgacgctgt cgctccggtc gggcgggtcg acggcctcgt cgagcgggac
     2401 ctggcgcggg cggcgcagcg tcgtacgccc cgtgttgcgc caggcgttga gcatgatcgt
     2461 caccagccag gctcgcggct gcagcgccgc gatccgcctc cggtcgtagc cccgcagcgc
     2521 ccggtaggca tgcagaaacg cctctgcggt gacgtcctcg gcgtctgccg gccgccggca
     2581 gacacccagc gccgtggagt acacgatgcg ctggtaggcg cggaagaggt cgacgaatcc
     2641 ggcgtcgagg tcgtccgcca gcctcgccag gatggccgtg acctcctcgg caccgtccgc
     2701 caccgtccgc tcacccctct atgtcgcttg cacccggtcg ccacgtccgc ggcccggtcg
     2761 ggccggtcgc cgcgtcgacc cctcgcagcg ggaaccgcat gtcaccctga accccgtccc
     2821 ggcccgaccg gttgcaacgg tggcgctcgg cgcggtcagc gggcggccgt gcctggcctc
     2881 cttcgcgtcc gccttcggcc accgaggacc tgggaccggt cagcagcatc cttgcgcgct
     2941 ggtgacgggc agggtggcac gcccggccgc cgggagctgg gcggccggcg cggcgccgcc
     3001 gggcttggtg gccttgacga tggcgccgtg gaggccgtcg ccgacctggt gggtgaaggt
     3061 gaccgagacg ccggcgaagc cggcggcggc gagaccctgc tcgtactcgg tcgcggcaag
     3121 ggtgccggcg atgcagccga cgtagctgcc ccgctcggcc cggtcgtcgg gggtcaggtg
     3181 gtcctcggcg acgacgtcgc tgatgcccag gcggccgcct ggcctgagga cgcggaacac
     3241 ctcggcgaag accgccggct tgtcgaccga caggttgacg acgcagttgg agatgaccac
     3301 gtcgacggtg ccggcgggca gggggatggc ctcgatctgg cccttgagga actcgacgtt
     3361 ggccgcgtcg gcctgctcgg cgttggcgcg ggccagggcg agcatctcct cggtcatgtc
     3421 cagcccgtag gccttgccgg tcggaccgac ccggcgggcg ctcagcagca cgtcgatgcc
     3481 gccgcccgag ccgaggtcga ggacggtctc cccctcgcgc agctcggcga cggcggtggg
     3541 gttgccgcag ccgaggctgg ccagcacggc cgtctcgggc agggcgtcgc ggtcaccggt
     3601 cgtgtagagc tcgccgccga acccgggctc ctcggcgctg gtgcagcagc cgctaccgca
     3661 gccgcagtcg acgggctggc cgcagccgca gtccgccggc tggccgcggc cgccctggcc
     3721 ggtgctgacg cgggtggcgg ccgccgcgta gcgggcgcgg acctgctcgc gcagctcgtc
     3781 gggggttggc tggccggtgt cggcggggct ggtggtcatg gggtggctcc ttcccgggcg
     3841 gtggcgccgt cggggtcgac gacggcggcc aggcggcgca gagtgttctg gtcgagcgag
     3901 tagtaggccc aggtgccgcg ctgctcgcgg cgcagcaggc cggcgaggac cagcttcttg
     3961 aggtggtggc tgacggtcgg ctgggacagg cccagggccg gggtgaggtc gcagacgcac
     4021 accgggtccg ggctcgtcgc gagcaggttg acgatcctga cccggtggcg gtcgccgagg
     4081 gccttgaaca ggtccgcggt cgcgcgggcc tcggcgtcgg tcaggctggt ggcggccagg
     4141 ggtgcacaac aggccgccgc cgaccccgac gccccgccac gggttgcggt ggccgtcggc
     4201 tcggccggcg ggtcggggag ctggtcgcgc atcgagcgcc tcccttggat cggctagatc
     4261 gggatcggct agatcgaaga acgtcgatga ttatacgtgt atgcatcgac gtctgtcaat
     4321 ctatatccgc cgaccaaggg cgcaacctgg ccggggttgc atcctcggct gatgcagtaa
     4381 atacttacta catgcgtgac agtcgatcga cactgctggc cgcggcggcg gaggagttcg
     4441 ccaggcacgg cccgaagggc gcccggatcc aggcgatcgt cgagcgggcc ggggtcaacg
     4501 agcgcatgat ctaccaccac ttcggcagca aggacggcct ctacgccgct gtgctggagg
     4561 ctcaggcgca cgggctggtc caggcgtggc tccagatcat cgacacggcc gccgagatgg
     4621 cgccgtacga gggcatgcgg gcggtgctgg tgggcttcgc cgacacgttc gcggcccggc
     4681 cgctgctggt ggccttgacg ctgcacgagg cccttggcgg gtggcggacc ctgccgttgc
     4741 cgaccgcgga catggcgccg ttccccgagc tgcgggggct gtacgagcga gggcagcgcg
     4801 agggggtgtt ccgctccgac tgcccgttcg agatcgccca cggcgtcgcc atgagcgcgc
     4861 tggtgggcag cgccgtgctc gcgccccggg gcgccgaggt cctcaaggcc gccgggctgg
     4921 acgccgatcc ggccaggctg cgtgaccagg tcgtcgggca gctgctggac ggcatgaccg
     4981 gaccgcccaa gcgaaccggc tgaccaaccc gcacctgccg gcataccgac acccagaggc
     5041 gcagtaaatg cttacggcag aagtcgagca aggagcccga cgatgaccac cctggacctt
     5101 tctcccgcca gcgccgcgcc gggtgctgtg aagacgagag cgctgcttgt ctgtggcgcg
     5161 gtcgccgggc cgctgttcac cgtcgcgtgg gtcgtggaag gcgccacgcg cgcccactac
     5221 aacccgttgc ggcatccggt cagctccctg gagctcggcg acttcggctg gacgcagcgc
     5281 gccaacttca tcgccgcggg cgggttgacg ctggccttcg ccagcgggct gcggcgtgcg
     5341 cggcggcccc tgggcggttc gacctgggga cccctgctgg ttggggccca cgcgatcggg
     5401 ctgctcggcg cggggatctt cgtcaccgat ccggtgagcg gctacccgcc cggaaccccg
     5461 accagctcca gaactacagc agcgtgcacg ccgtcctcca cgacctgttc tcggtcggta
     5521 ccttcgtggg actgcccgtc gcctgcttcg tgttcgcccg gcgcttcgcc ggatggggtg
     5581 agcgcggatg ggcgatctac tcggccgtga ccggtgtcgt cttcacggtc gcgttcgtcc
     5641 ttgccagcat ggcctttggc caggccgagc gcctggtggc tctcggcgga ctgttccagc
     5701 gcatcaccat caccgtcggc tggggctggc tgaccctgct cgccgtccac ctgctacgag
     5761 acctgtccca acgaccggga aggatgcgaa cgtaggctca cgtgcggacc tggcggcgct
     5821 cgccgccggc ttcgcgcttg atcgcgcagg cgttgcgggg cagccacccg agcgacgccc
     5881 ggcccgaaga gcagcggctt gtctgccatc gaatcgccca gataggctcc gcgcctcaag
     5941 ctgacgactg gaggctgcgt gcggcaactg accgtcctgc tcggagtctt tgctgtcgtg
     6001 gcgggcgtcc tggccgcgcc agccgcggcc gacgtcacgc gctccaccgt tcacgggacc
     6061 agtgcgtacg cgatgttcga ggttcctggc ggcaccttgg tgatcagcgc cttcgacgcc
     6121 aacgcctatc aggacggcac cggcgtcagc cacgccgcgt tcgtctatgc cgaactcttc
     6181 agcctcgagt gcacgcccga cgggctcacc tgcaccggca aggactggct cgcctcggcc
     6241 accctggacg acggcgacgc tgtgcgcatc gccggggacc tgtggacggc ctcgctcggg
     6301 ccgctggtgg tcgacgcccg caagctcacc tgcatcgaca cctatgagcc gggcggtgac
     6361 gggtttcccg tgtcgaccag ctgcactcag gtgaccgagc cgaccacctt gtcggggtca
     6421 tgggcggcga ccgggcagat tgcggactac cacgactcgc gggtgacgga ccaggatggc
     6481 gtgcacacgg tctttaccgc ggctggcgtg tcccgaccgg cgaccgcgac cgccgaggcg
     6541 ttcggcacca gtt
//
LOCUS       DASPYY010000387         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1049853, whole genome shotgun sequence.
ACCESSION   DASPYY010000387 DASPYY010000000
VERSION     DASPYY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049853"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VEP73_06020"
     CDS             <1..219
                     /locus_tag="VEP73_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26020.1"
                     /translation="ADGSKWGTTAFSRICPPGEIDLLVTDDQAPEEQVRQFRAAGVEV
                     VCVQSGAAPGEEPASSYGASAHGGGTRP"
     gene            216..1403
                     /locus_tag="VEP73_06025"
     CDS             216..1403
                     /locus_tag="VEP73_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26021.1"
                     /translation="MSARQPGATVARTDLRLTRARVATATVFALHAVVFGSWTPHIPL
                     VKDRIGLHDGTLGLALLGAPVGSVLAMTVVGTVIARWGSRRVMIATMVGYGLAAPALG
                     LADSLARLFLALLLLGAFQGSLDIAMNAQAVAVETAYGRPIMSSFHACWSLGAFVGAG
                     IGTVAVGLGVGLAPQLLAIGVAFALASPYLARPLVRDDQATDDHHRIVLPWGNRWLLL
                     LGGIMFASLLCEGAAADWTAVYLHDALSAPTEVAGLGYVSFAVAMFAGRAMGDRWVAR
                     YGGGRLVGVLAGAGAVALGTALLLGRPVPALAGLAVYGLGLALVVPVCFSAAAATGGS
                     HAGHSIAAVATAGWVGFLLGPPAIGSLARATSLPLALGVLPALCAGIALGAPHLRTRP
                     ARR"
     gene            1899..>2435
                     /locus_tag="VEP73_06030"
     CDS             1899..>2435
                     /locus_tag="VEP73_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HYT26022.1"
                     /translation="MERAYRTHRTSPHLSETTRERLTRRQSTPPDLVVVSPLGDWACA
                     DCEGTGDLLVMEDDRPLCLPCADLDHLVFLASGDAALTRRAKKASTLSAVVVRFSRAR
                     KRYERQGVLVEQAALEAAEQQCLGDEGARQRRRERDRQRRADQDVALVSRMAREIARL
                     FPGCPAPRAEAIAAPTAVR"
BASE COUNT          269 a          909 c          903 g          354 t
ORIGIN      
        1 gccgacgggt cgaagtgggg gaccacggcg ttcagccgga tctgcccacc gggcgagatc
       61 gacctgctcg tcaccgatga ccaggccccc gaggaacagg tccgccagtt ccgcgcggcc
      121 ggcgtggagg tcgtctgtgt ccagagcggc gccgcgcccg gcgaggagcc ggcgagcagc
      181 tacggcgcat ctgcgcacgg gggcgggacg cgaccgtgag cgcccggcag cccggcgcca
      241 cggtcgcccg gaccgaccta cgcctcaccc gcgcccgcgt cgcgaccgcg accgtcttcg
      301 cgctccacgc cgtggtgttc ggttcgtgga caccgcacat cccgctcgtc aaggaccgca
      361 tcggcctgca cgacggcacg ctcggactgg ccctgctcgg cgccccggtc gggtcggtgc
      421 tcgcgatgac cgtcgtcggc accgtcatcg cccgatgggg cagccggcgg gtcatgatcg
      481 cgaccatggt gggatacggc ctggccgccc cggcgctggg tctggccgac tccctggccc
      541 ggctgttcct cgcgctcctg ctgctcggcg ccttccaggg ttcgctcgac atcgcgatga
      601 acgcgcaggc cgtcgcggtc gagaccgcct acggccggcc gatcatgtcg agcttccacg
      661 cctgctggag ccttggcgcg ttcgtcggcg cgggcatcgg cacggtcgcc gtcgggctcg
      721 gcgtcgggct cgcgccccag ctgctcgcga tcggggtcgc gttcgcgctg gccagcccgt
      781 acctcgcccg cccgctggtg cgcgacgacc aggcgaccga cgaccaccac cgcatcgtcc
      841 tgccatgggg caaccgctgg ttgctgctgc tcggcgggat catgttcgcc agcctgctgt
      901 gcgagggcgc ggcggccgac tggacagccg tctacctcca cgacgcgctg tcggctccca
      961 ccgaggtcgc cggcctcggg tacgtctcgt tcgccgtggc gatgttcgcg gggcgtgcga
     1021 tgggcgaccg gtgggtcgcg cggtacggcg gcgggcgcct ggtcggggtg ctcgcgggtg
     1081 ccggagcggt ggcgctcggc accgccctgc tgctcggccg cccggtgcct gccctggccg
     1141 ggttggcggt gtacgggctc ggcctggccc tcgtcgtccc ggtgtgcttc agcgcggcgg
     1201 ccgcgacggg cggcagccac gccgggcact cgatcgccgc ggtggcgacc gcgggctggg
     1261 tcgggttcct gctcgggccg ccagccatcg gctccctggc acgcgcgacc tcgctgccgc
     1321 tcgcgctggg ggtgctcccc gccctctgcg ccgggatcgc gctcggcgca ccccaccttc
     1381 gcacccggcc ggcacgccgc tgaccagctc tgcccaaggg gtggtgggac gcctcgccgc
     1441 gccgtgaggc ggccagcgag ccatttccgc cgcgcggcaa gattcaggcc ggaccagtcg
     1501 gcgctcaccg gccgatcctg gacggcgccg tcggacgact atgcttctgc cgtcatgggc
     1561 gaagggccat ccgggtgaac caggatcccc attccaagct ggagcatcgc gtcgtcgccg
     1621 ccgcgcaggc cgcgctggca cgccaggggt ccgtctcgcc gcttgacgtc cttgcgggga
     1681 ttgggtggct gccatccggc ctggtcgacg actggcggcg cgggcgcgtc ggcccgctgg
     1741 aacgggtcgc agcggtaggc ccggaacgcc tggccgccgc attggggatc ttccaccggt
     1801 gggcggagtc cgccggtctg cgaccaggcg aggtcgccta cgtcgccgcc acccgggatc
     1861 ggcggccgct gcgcttcacc gcggacggcg accctgaggt ggagcgggcc taccgcacgc
     1921 accggacttc gccgcacctt tcagagacca cgcgggagcg gttgacacgg cggcagagca
     1981 cgccacccga tctggtggtc gtctcgccgc tcggggactg ggcctgcgcc gactgcgagg
     2041 gcaccggcga cctgctggtc atggaggatg acaggcccct gtgcctgccc tgcgcggacc
     2101 tggaccacct ggtgtttctc gcttcggggg atgctgcgct gacccggcgc gccaagaagg
     2161 ccagcaccct gtcggccgtg gtcgtgcggt tcagccgcgc ccgcaagcgc tacgagcggc
     2221 agggcgtcct tgtcgagcag gcggcgctcg aagctgccga gcagcagtgt ctcggcgacg
     2281 aaggcgcccg ccagcggcgt cgggaacgcg atcgccagcg ccgagccgac caggacgtgg
     2341 cgctcgtgtc acggatggct cgggagatcg cccggctctt tccgggctgc cctgcgccgc
     2401 gggccgaggc gatcgccgcc cccaccgccg ttcgg
//
LOCUS       DASPYY010000388         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_768489, whole genome shotgun sequence.
ACCESSION   DASPYY010000388 DASPYY010000000
VERSION     DASPYY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768489"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1434)
                     /gene="hflX"
                     /locus_tag="VEP73_06035"
     CDS             complement(4..1434)
                     /gene="hflX"
                     /locus_tag="VEP73_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYT26023.1"
                     /translation="MDPMDPVTDQVPADVRRDDTDIKLVELRKLRLERVVLVGVAVGG
                     SIEASERSIEELRRLAETAGAEVLEAMLQRRDRPDAGTFVGKGKANELAARVRALGAD
                     TVIMDDELSPGQLRQLEELVDAKVIDRTALILDIFAQHATSREGKAQVELAQLSYLLP
                     RLRGWGESLSRAGGGIGTRGPGRGPGETKMELDRRRIRRRITKLRRDLVDLARTRDVK
                     RQRRERAGAPAVALAGYTNAGKSTLLNRLSGAGVLVEDKLFSTLDPTTRRLDLPDGRA
                     ATLTDTVGFIAKLPHDLVEAFKSTLEEVGRADLIVHLVDASQLDPAAQVGAVRAVLGE
                     VGTADVPELLVLNKVDLVDEVTRARLARQYPGAPLVSAATGEGVDRLLADLAVRLPHP
                     EVLLTVLVPFERGGLVDRVHREGEVLAVEHLPEGTRIAVRAPHALAAALGPYALSPDG
                     QAAPADGRAAPATAAPSARGPVVRGS"
BASE COUNT          190 a          594 c          598 g          220 t
ORIGIN      
        1 agctcaggag cctcggacga ccggccctcg cgcgctcggt gcggcggtgg caggtgccgc
       61 acggccgtcc gcgggcgctg cctggccgtc cggagagagc gcgtagggcc cgagggcggc
      121 agccagcgcg tgcggcgcgc gcacggcgat ccgggtgcct tccggcaggt gctcgaccgc
      181 cagcacctcg ccctcccggt ggacacggtc gaccaggccg ccgcgctcga aggggacgag
      241 gacggtgagc agcacctcgg ggtgcggcag ccgcaccgcg aggtcggcca gcaggcggtc
      301 gaccccctcg ccggtggccg ccgacaccag cggcgcgccc gggtactgcc gggcgagccg
      361 cgcgcgggtc acctcatcga ccaggtcgac cttgttcagc acgagcagct cgggcacatc
      421 ggccgtgccc acctccccga ggaccgcccg cacggcgccg acctgcgcgg ccgggtcgag
      481 ctgggaggcg tcgaccaggt ggacgatcag gtcggccctg cccacctcct cgagggtgga
      541 cttgaacgcc tcgaccaggt cgtggggcag cttggcgatg aacccgaccg tgtccgtgag
      601 cgtcgccgcg cggccgtcgg gcaggtccag ccgcctcgtg gtcgggtcca gggtcgagaa
      661 cagcttgtcc tcgaccagca cgcccgcgcc ggacagccgg ttcagcagcg tggacttgcc
      721 cgcgttggtg tagccggcga gggcgacggc gggggcgccg gcccgctcgc ggcgctggcg
      781 cttgacgtcc ctggtgcgtg ccaggtcgac caggtcgcgg cggagcttgg tgatccgccg
      841 ccggatgcgg cgccggtcga gctccatctt cgtctcgcca gggcctcggc ccgggcccct
      901 ggtcccgatc ccgccgcccg cgcggctcag gctctcgccc cagccgcgca accggggcag
      961 caggtagctg agctgcgcca gctcgacctg tgccttgccc tcccggctgg tcgcgtgctg
     1021 ggcgaagatg tcgaggatca gcgcggtgcg gtcgatcacc ttcgcgtcga ccagctcctc
     1081 gagctggcgg agctggcccg gtgacagctc gtcgtccatg atcacggtgt cggcgccgag
     1141 cgccctgacc ctggcggcga gctcgttcgc cttgcccttg ccgacgaacg tgccggcgtc
     1201 gggccggtcg cggcgctgca gcatggcctc gagcacctct gccccggcgg tctcggcaag
     1261 gcgccgcagc tcctcgatgg agcgctcgga cgcctcgatg gagccgccca cggccacgcc
     1321 gacgagcacg acccgctcga ggcggagctt gcgcagctcg accagcttga tgtcggtgtc
     1381 gtcgcggcgg acgtcggcgg gaacctggtc ggtcacgggg tccatgggat ccatgggctc
     1441 cacggtagcg ggtcgggcgc gatccgtccc gcgggtttcc tcgggcaccg tctacctgac
     1501 cagcgccttg cggacccgct cgcagccctc ctcgaggcgg gcgtccggga tggtgagcga
     1561 gagccggatg tacccctcgc cgctcgggcc gtagccggac cc
//
LOCUS       DASPYY010000389         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2315002, whole genome shotgun sequence.
ACCESSION   DASPYY010000389 DASPYY010000000
VERSION     DASPYY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315002"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VEP73_06040"
     CDS             complement(<1..1341)
                     /locus_tag="VEP73_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYT26024.1"
                     /translation="MRQPVSRGLGPSAEAPAPELVEAGFALEVADAPLLHHGLNLADM
                     AHVLDLRARRVMPEEPARRLLRLLLVAHQTPAAEFPYDPVHGEVYNCRERFFVERLGD
                     DAGWLHAGRPRREAGRVALRLLLRVLAAELVSDAAGFARAVAGQAHEHAETLMPDQTY
                     LQQAQPSTFGHYVLAFAYPALRDAERLLQGLDWVDRSPAGAGCVNGSRLLADRTRPAG
                     LLGFPAVIEHTRDAMWQTDGLVDFLATSASLVTNQSKLAEDLEIWDSQEFDFVDLAGP
                     YTRASVLMPQKRNPYALSIVRGAAGILIGRLSGFLAVVKTPSARSDNLIFAYGEVPRA
                     LDLALRATRLSTGVIRTLQVNAERMRAALDSGFSQATDLAEYIMQSCAIDYRSAYRVV
                     GHTVRQASAAGLAGADIDGAMLDAAATEVTGHPLGLAGRDLSAALDPWQIVASRT"
     gene            complement(1326..>2260)
                     /locus_tag="VEP73_06045"
     CDS             complement(1326..>2260)
                     /locus_tag="VEP73_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT26025.1"
                     /translation="APPLYVKRDDLTGFALAGNKARQLELLVGDALGRGRDALVTGGG
                     QGSNFCQAAAVAARVAGLGCVLVMYGDEPASPHPNLALARASGAEIRFTGRPERESVD
                     PALDEAAAELEAEGRRPYLVPRGGASGVGAAGYALAAAELATQLDSGRIAPELVLVAA
                     GSGGTQAGLVAGAVAWGHPWRVAGAAVSRPAAECADQVLRLARAAAALLRQPGADAGH
                     VEARHVEARHVEVWDARGPGYGIPSPEGERAARVAADTEGLLLDPVFTSKALALLPRL
                     VHDGVRGPVVFWHTGGTPDALAHLTRGGGRCGNR"
BASE COUNT          269 a          827 c          897 g          267 t
ORIGIN      
        1 ggtccggctg gccacgatct gccacgggtc cagggccgcg gacaggtcgc ggccggccag
       61 gccgagcggg tgcccggtga cctcggtggc cgcggcgtcg agcatggccc cgtcgatgtc
      121 ggcgccggct agcccggccg cgctggcctg cctgaccgtg tgcccgacca cccggtaggc
      181 gcttcggtag tcgatggcgc agctctgcat gatgtattcg gccaggtccg tagcttgcga
      241 gaagccggag tcgagcgcgg cgcgcatccg ttcggcgttc acctggaggg tgcggatcac
      301 gccggtgctc agccgggtgg cgcgcagcgc gaggtcgagc gcccggggca cctccccgta
      361 ggcgaagatc aggttgtcgc tgcgtgccga cggcgtcttg accaccgcca ggaacccgga
      421 gaggcgcccg atgaggatgc cggccgcgcc ccgcacgatg gacagcgcgt acgggttgcg
      481 cttctgcggc atgagcacgc tggcccgggt gtaggggccg gccaggtcga cgaagtcgaa
      541 ctcctggctg tcccagatct cgaggtcctc ggccagcttg ctctggttgg tgacgaggct
      601 ggccgacgtg gccaggaagt cgacgaggcc gtcggtctgc cacatcgcgt cgcgggtgtg
      661 ctcgatgacg gcggggaagc cgagcaggcc ggccggccgg gtccggtcgg ccagcagccg
      721 gctgccgttg acgcagcccg cgccggccgg gctgcggtcc acccagtcga gcccctgcag
      781 caggcgctcg gcgtcgcgca gcgccgggta ggcgaaggcg agcacgtagt ggccgaaggt
      841 ggacggctgc gcctgctgca ggtaggtctg gtcgggcatg agggtctcgg cgtgctcgtg
      901 ggcctgcccg gccaccgccc gggcgaagcc ggccgcgtcc gacaccagct cggccgcgag
      961 gacccgcagg agcagccgga gcgccacccg gccggcctcg cgccgcggcc gcccggcgtg
     1021 cagccagccg gcgtcgtcgc cgagccgctc gacgaagaac cgctcgcggc agttgtagac
     1081 ctcgccgtgg accgggtcgt aggggaactc ggcggccggc gtctggtggg cgacgagcag
     1141 gagccggagc agccggcggg ccggctcctc cggcatcacc cggcgggcgc ggaggtccag
     1201 gacgtgcgcc atgtcggcca ggttcagccc gtggtggagc agcggcgcgt cggccacctc
     1261 gagggcgaag ccggcctcga ccagctccgg ggcgggcgcc tccgccgacg gccccagccc
     1321 cctgctcacc ggttgccgca ccgcccacct cctcgcgtca ggtgcgcgag cgcatcggga
     1381 gtcccgccgg tgtgccagaa gacgaccgga ccgcggacgc cgtcgtgcac gaggcggggc
     1441 aggagggcga gcgccttgga cgtgaacacc gggtcgagca ggaggccttc ggtgtcggcg
     1501 gccacccggg cggcccgctc gccctccggg gagggaatcc cgtagcccgg gccgcgggcg
     1561 tcccacactt cgacgtgcct cgcttcgacg tgcctcgctt cgacgtgccc ggcatccgcg
     1621 cccggctggc ggaggagcgc cgcggcggcg cgggcgagcc gcagcacctg gtcggcgcac
     1681 tcggccgccg ggcggctgac cgccgcgccc gccacccgcc aggggtggcc ccaggccacc
     1741 gcgccggcga ccaggccggc ctgggtgccg ccggagccgg cggcgaccag gaccagctct
     1801 ggcgcgatcc gcccgctgtc gagctgggtg gcgagctcgg cggcggccag tgcgtacccg
     1861 gccgcgccga cgccgctggc cccgccgcgg gggaccaggt acggccggcg cccctcggcc
     1921 tcgagctcgg ccgccgcctc gtccagggcc gggtccacgg actcgcgttc cggtcggccg
     1981 gtgaagcgga tctcggcccc ggaggcgcgg gcgagcgcga ggttggggtg tggcgaggcc
     2041 ggctcgtcgc catacatgac gaggacgcag ccgaggccgg cgacccgggc cgccaccgcc
     2101 gccgcctggc agaagttcga gccctggccg cccccggtga cgagcgcgtc gcggccgcgc
     2161 ccgagcgcgt cgccgacgag cagctcgagc tggcgggcct tgttgcccgc gagcgcgaac
     2221 ccggtgaggt cgtcgcgctt gacgtacagg ggcggggcgc
//
LOCUS       DASPYY010000390         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2595219, whole genome shotgun sequence.
ACCESSION   DASPYY010000390 DASPYY010000000
VERSION     DASPYY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595219"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VEP73_06050"
     CDS             complement(<1..747)
                     /locus_tag="VEP73_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HYT26026.1"
                     /translation="MSVSGTVLGLGCGQPHGSAEILLKAALRAAEAEGAQVELVRLDE
                     LRLPSGAAEGAEPDDAWWLWERLVGCDGLIVSTPIISRTVAARLKLFGDRLLGPNADA
                     AIIEGLTALRREGREPAVPFRVDERVLKPRVAGFLAVGGSLTSQWKTLALPIMHTTTF
                     SMHIAVVDQVQFEGAGTPRSVLLDPAAIDRAALLGRNVASQLGVAFEAARYLGPPGLC
                     PMCHLDVVGLRGADVECATCGARGLLAPDLT"
     gene            818..1603
                     /locus_tag="VEP73_06055"
     CDS             818..1603
                     /locus_tag="VEP73_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26027.1"
                     /translation="MRTQGPQGRLDGKRALISGTGGGIGRATALLCAREGAHVVGCDL
                     HPETSEETVGLVRAGGGRMDAIAPVDLASEEGARRWVDAAADLAGGIDILVNNASAIR
                     FGPIGELSFADWSFTIRNELDIVFLVTRAAWPHLVAAGGGSIVNVASITASRGAFFMP
                     QNAHGAAKGGVLALTYQLVVEGGPHGIRVNAVSPAMTETPQTAPLLHDPDGPAASIAA
                     RVPLGRWGQPEDVAQAILFLASDEASHVSGANIPVDGGAAVVG"
BASE COUNT          223 a          604 c          600 g          211 t
ORIGIN      
        1 ggtcaggtct ggcgccagca ggccgcgggc gccgcaggtg gcgcactcga cgtccgcgcc
       61 gcgcaggccg acgacgtcga ggtggcacat cgggcagagc ccgggcggcc ccaggtagcg
      121 cgccgcctcg aacgccacgc cgagctggct ggcgacgttg cgcccgagca gcgccgcccg
      181 gtcgatcgcc gccgggtcga gcagcaccga gcgcggcgtg ccggccccct cgaactgcac
      241 ctggtcgaca acggcgatgt gcatcgagaa cgtcgtggtg tgcatgatcg gcagcgccag
      301 cgtcttccac tgcgaggtca gcgagccgcc taccgcgagg aagccggcca cccggggttt
      361 gagcacccgc tcgtcgacgc ggaacgggac ggcaggctcc cgcccctccc ggcgcagcgc
      421 ggtcagcccc tcgatgatgg cggcgtcggc gttcggcccg agcagccggt cgccgaacag
      481 cttgagccgg gccgccaccg tccggctgat gatcggggtg ctcacgatca gcccgtcgca
      541 cccgactagc cgctcccata gccaccacgc gtcgtccggc tcggcgccct cggcggcgcc
      601 gcttggcagc ctgagctcgt cgagccggac gagctcgacc tgcgcgcctt cggcctcggc
      661 ggcccgcagc gcggccttca gcaggatctc cgcgctgccg tgcggctggc cgcagcccag
      721 cccgagcacc gtaccgctca ccgacatcgg ttcaccgtcc tcgtcaaaat tcccgcaaga
      781 ctggcgatat tcactctatg gaagttacta tcttacagtg agaacgcaag gcccgcaggg
      841 ccggctggac ggcaagcggg cgctcatctc gggtaccggc ggcgggatcg ggcgggcgac
      901 ggcgctgctc tgcgcgcggg agggcgccca cgtggtgggc tgtgacctgc acccggagac
      961 gtccgaggag acggtcgggc tggtccgggc cggtggcggg cggatggacg cgatcgcccc
     1021 ggtcgacctg gcctccgagg agggcgcccg gcggtgggtc gacgccgcgg cggacctcgc
     1081 cgggggcatc gacatcctgg tcaacaacgc gtcggcgatc cggttcggcc cgatcggcga
     1141 gctgtccttc gccgactggt cgttcacgat caggaacgag ctcgacatcg tcttcctcgt
     1201 cacccgcgcg gcctggccgc acctagtcgc cgccggcggc gggtcgatcg tgaacgtcgc
     1261 gtcgatcacc gcctcgcgcg gcgcgttctt catgccgcag aacgcgcacg gcgcggccaa
     1321 gggcggcgtg ctggcgctca cctaccagct ggtcgtcgag ggcgggccgc acggcatccg
     1381 ggtcaacgcc gtcagcccgg cgatgaccga gacgccgcag acggcgccgc tgctgcacga
     1441 ccccgacggc ccggccgcga gcatcgcggc ccgcgtgccg ctgggccggt ggggacagcc
     1501 ggaagacgtc gcccaggcga tcttgttcct ggcctcggac gaggcgtccc acgtgagcgg
     1561 cgcgaacatc cccgtcgacg gcggcgcggc ggtggtcgga tgacggcccg gccggccacg
     1621 gggccgcgcg aaaggctg
//
LOCUS       DASPYY010000391         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_909762, whole genome shotgun sequence.
ACCESSION   DASPYY010000391 DASPYY010000000
VERSION     DASPYY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909762"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VEP73_06060"
     CDS             complement(<1..1114)
                     /locus_tag="VEP73_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYT26028.1"
                     /translation="MDASSYDETRHDRRRARRPGPVLAAVDDSNLAAAVGRCAAEHAR
                     ISGRTLVVVSVVPVGDARGLREGGQLPGGLDDYDDALAIARRVGPTLDELAVAYQLEI
                     RGYLARGGPRRQARRIAATVLRVAGETGAEVIVVGEPRDRAPAGMSVSVRIAKGAPTR
                     VLVLLEQPEPRRAVRRIRRRAGVAPVADHDLVGTGGVARAGRLAGDPSEVILTRQGRR
                     LLAERAYRLRTHVLPELRAALDDRERDGRGDTEYERAVDELRRLSWLVEHAADAEELP
                     DDPDVVELGETVTVQIGGGALERFLIVHPVEAPLDNTRISAHSPLARALLGRRIGDEV
                     EVDAPVGTYRCRILAAERTPRSAARQVGTPRQPTEVT"
     gene            complement(2195..>2390)
                     /locus_tag="VEP73_06065"
     CDS             complement(2195..>2390)
                     /locus_tag="VEP73_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26029.1"
                     /translation="RLDNRPTRRDRRSPGQHVTSYLTVYNTIRPHEAIAFATPLVRYL
                     QAPSIPPRANLHQPGSVSDS"
BASE COUNT          324 a          847 c          918 g          301 t
ORIGIN      
        1 gggtcacctc cgtgggctgc cgtggcgtgc ccacctgacg cgccgcggac ctgggcgtgc
       61 gctcggcggc gaggatccgg cagcggtaag tcccgacggg cgcgtcgacc tcgacctcgt
      121 cgccgatgcg gcggccgagc agtgcccgtg ccagcggcga gtgggccgag atgcgggtgt
      181 tgtccagggg cgcctcgacc gggtggacga tcaggaaccg ctcgagcgcg ccgccgccga
      241 tctggacggt gacggtctcg cccagctcga ccacgtcggg gtcgtcgggc agctcctcgg
      301 cgtcagcggc gtgctccacc agccaggaca gccgacgcag ctcgtcgact gcccgctcgt
      361 actcggtgtc gccgcggccg tcgcgctcgc ggtcgtcgag cgcggcgcgc agctcgggca
      421 ggacgtgggt gcgcagccgg taggcgcgct cggccagcag gcgcctgccc tgccgggtca
      481 ggatcacctc gctcgggtcg ccggcgaggc ggccggcacg cgcgacgcct ccggtgccaa
      541 ccaggtcatg gtcagccacc ggtgcgacgc cggcgcggcg acggatccgg cggacggccc
      601 gtcgcggttc tggctgctcg agcaagacca gcacgcgcgt gggcgcgccc ttggcgatcc
      661 gcacggacac gctcatcccg gcgggcgctc gatcgcgggg ctcgccgacc acgatcacct
      721 ccgcgccagt ctccccggcc acccgtagca ccgtcgccgc gatccggcgg gcttgccgtc
      781 gcggcccgcc gcgggccaga tagccgcgaa tctcaagctg gtaggcaacc gcgagctcgt
      841 cgagtgtcgg cccgaccctc cgagcgatcg cgagggcgtc gtcgtagtcg tcgaggccgc
      901 cgggcagctg tccaccctcc cggagtccgc gcgcatcccc gactggcacc accgagacga
      961 cgaccagcgt ccgtccactg attcgggcgt gctcggcggc gcaccggccg accgccgcgg
     1021 cgaggttgga gtcgtcgacg gcggccagca ccgggccggg ccggcgcgcc cgtcgccggt
     1081 cgtggcgggt ctcgtcgtag ctgcttgcgt ccatgcaggc ctcctccagg tcgggcgccg
     1141 atggtgatca agggaggtgg gcttgagtgg tgggacggtc gcgcggacgg gtgcccaaca
     1201 tgggtgtcca agccggagac gcgcccgagc agcgggagcc tcgccgccag cgccaggctc
     1261 ggcgccggtc ggcggcggcg atgcgcggct ccatccgagc cgtgacgggc cgcccgcgca
     1321 gcgtcgccct cccgccgagc agctaccgcg tgatgccacg gacccgtagg cgggcccgac
     1381 gggccacggc ggcgtgggcc agctcgcaag cgaggagcag gactgcaccc gccacgccga
     1441 ccagctagta ggtcccggcc gtctggctgg ggaaccgtcc cggcagcagc agtacctgcg
     1501 cgaccagtga tcaggcgagc aggcccggga ccgctgcgac gcaggagcgg gcgccgaccg
     1561 ggatccgcgc cgaccggagc gccgggcgcc gccgcgcgac gcacggcggc gccgtgcccg
     1621 gtaccggaca cgggcgcgcc ccggctgcgg caccgcgggg gcatggcccc ggcccgcgcg
     1681 cgccggacgc gtggtatcgg catgctgtgc gtgccacctc atggctggcg gcagcgcggc
     1741 gggcgtcgag acagcgggca taagcatgag cccgaggtcg ttgtcgagcg gtagcgccag
     1801 ctcgggcagg tagtccccgg cgagcagcgc gaaggcgagc aacgctacac cggacagcgt
     1861 gtggcgggcg acggcgaccg ccacccaggt ctgggaagcc gcagcgatcc ggatcagaag
     1921 aggcactcgg gcaagcctcc aaggctggcc ggacgccaac gccaaggtcc taacggcacc
     1981 cgaagttctc gctcgcccat tgctgggtgc ggaccgggcg ggcccccgac ccactgtcct
     2041 gacggctccg cggccgagga cactgctcag tggactactc ccttcgcgac tcaggatacc
     2101 ggccgggtgt aagaccgccc taaaggacac gggcgcagga tggcgaggca ggttgccgga
     2161 gtgtggtgcg gtcggacatg cgtcctgtcc cgcgtcaaga atctgagaca gatccgggct
     2221 ggtgaaggtt ggcccttggc gggatggacg gggcttgcag gtagcggacg agcggcgtgg
     2281 cgaaggcgat cgcctcgtgg gggcggatgg tgttgtagac ggtcaggtag ctggtgacgt
     2341 gctggcctgg ggaccgtcga tcccttcgag tcggccggtt gtcgagcctc
//
LOCUS       DASPYY010000392         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2315422, whole genome shotgun sequence.
ACCESSION   DASPYY010000392 DASPYY010000000
VERSION     DASPYY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315422"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VEP73_06070"
     CDS             complement(<1..963)
                     /locus_tag="VEP73_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT26030.1"
                     /translation="MTAAEGGGRARCRALTAKGERCRNPAGPAGLCARHEQRLAEVAA
                     DAAATDRAGAETAGGPDAEVAEALAPFLGWAEEALEFLRRRLTGDYQVDEFGYDPDLV
                     RHVLAPLLRPLYRYWWRVEVRGLEHVPDAGAALVVGNHAGTLPFDALMVALALLDEHP
                     AHRTLRMLAADLAFTMPVVAPLARKSGNTLACSEDARRLLEAGELVGVWPEGYKGLGK
                     RFRERYRLRRFGRGGFVEVALAAGAPIVPVAVVGSEEIYPMLANLRRVARLLGVPYVP
                     VTPTFPLLGPLGLVPFPSRWVIEFCPPVETAGLGPDAVLDPMVVF"
     gene            complement(960..>1625)
                     /locus_tag="VEP73_06075"
     CDS             complement(960..>1625)
                     /locus_tag="VEP73_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26031.1"
                     /translation="GDPAVWRESMDPGTPRHGFGKDSIEVEDYARSFDRRRPDVAVTV
                     LRLANQVGPTVDNPLTRYFALPVCPVVFGFDPRLQFLHSDDAQAVLLRAVLGPAAAGT
                     FNVAGDGVVLLSQAIRIAGRPSLPIPEPLVSSVTELLRRTVDFSPEQVRYLQFGRVAD
                     TTAAREELGFSPARSSLAAFEDFAATRLHRLVTQERVDRLERDALAALRRAAGWLSGG
                     VGT"
BASE COUNT          221 a          602 c          635 g          167 t
ORIGIN      
        1 gaacaccacc atggggtcga ggaccgcgtc gggcccgagg ccggcggtct cgaccggcgg
       61 gcagaactcg atcacccacc tggaggggaa cgggaccagc ccgagcgggc cgagcagggg
      121 gaacgtcggg gtcacgggca cgtacggcac cccgaggagg cgggccaccc gccgcaggtt
      181 ggcgagcatc gggtagatct cctcgctgcc gaccaccgcc accgggacga tcggcgcgcc
      241 ggcggcgagc gccacctcga cgaagccgcc gcgcccgaag cgccgcaggc ggtagcgctc
      301 gcggaaccgc ttgccgagcc ccttgtagcc ctccggccag acgccgacca gctccccggc
      361 ctcgagcagg cgccgggcgt cctcgctgca cgccagggtg ttgcccgact tgcgggcgag
      421 cggggccacc accggcatcg tgaaggcgag gtcggcggcg agcatccgca gggtccggtg
      481 ggccgggtgc tcgtcgagca gcgcgagggc gaccatcagg gcgtcgaagg gcagcgtgcc
      541 ggcgtggttg cccacgacca gggcggcgcc cgcgtccggc acgtgctcga gcccgcgcac
      601 ctcgacgcgc caccagtacc ggtagagcgg gcggagcagc ggggcgagga cgtgccggac
      661 caggtcgggg tcgtagccga actcgtcgac ctggtagtcg ccggtcagcc ggcggcggag
      721 gaactcgagc gcctcctcgg cccagccgag gaacggcgcc agcgcctcgg ccacctcggc
      781 gtcgggcccg ccggcggtct ccgcgccggc cctgtcggtg gctgccgcgt cggcggccac
      841 ctcggcgagc cgctgctcgt ggcgggcgca gaggccggcg gggccggccg ggttgcggca
      901 ccgctccccc ttcgcggtca gcgcgcggca gcgggcgcgc ccgccgccct cggccgcggt
      961 cacgtcccca ccccgcccga gagccagccg gcggccctgc ggagggccgc gagcgcgtcc
     1021 cgctcgagcc ggtccacccg ctcctgggtg acgaggcggt gcaggcgcgt ggcggcgaag
     1081 tcctcgaacg cggccaggga ggaccgggcg ggcgagaagc cgagctcctc cctggccgcc
     1141 gtcgtgtcgg ccacgcggcc gaactgtaga tatcggacct gctcgggcga gaagtcgacc
     1201 gtgcgccgca gcagctcggt cacgctcgac acgagcggct cggggatcgg cagcgacggc
     1261 ctccccgcga tccggatggc ctggctgagc agcaccacgc cgtccccggc gacgttgaac
     1321 gtgccggcgg cggccgggcc gaggaccgcg cgcagcagca ccgcctgggc gtcgtcggag
     1381 tggaggaact gcagccgggg atcgaagccg aacaccaccg ggcagaccgg cagggcgaag
     1441 taccgggtga gggggttgtc gaccgtcggc ccgacctggt tggcgagccg cagcacggtg
     1501 accgccacgt cgggccggcg ccggtcgaac gagcgggcgt agtcctcgac ctcgatcgag
     1561 tccttgccga agccgtggcg cggcgtgcca gggtccatcg actcccgcca cacggccggg
     1621 tcgcc
//
LOCUS       DASPYY010000393         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1752983, whole genome shotgun sequence.
ACCESSION   DASPYY010000393 DASPYY010000000
VERSION     DASPYY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752983"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..692)
                     /locus_tag="VEP73_06080"
     CDS             complement(129..692)
                     /locus_tag="VEP73_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYT26032.1"
                     /translation="MRGTRDWVQQGTVRAELEAAESRTLAPWAARAAESAGRGRPEPE
                     DPVRTCWMRDLDRIIFSRAMMRAHGKTQSFIPAFSEGPAAGRQGGPYIGDHFVTRATH
                     MQHTARIAATIAQALALNVPLASAIATGHDLGHACFGHGGEAALQAVAPSGFDHAAQG
                     ARLTGGGTSSIPPSTPARSASVRSAVR"
     gene            857..1408
                     /locus_tag="VEP73_06085"
     CDS             857..1408
                     /locus_tag="VEP73_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HYT26033.1"
                     /translation="MVHDLLSLPVPPLEKVLRSVAVYLFLLFALRLSGKREVAQLNPF
                     DFVVLLLLSNTVQNAIIGNDDSLLGGVLGAAVLLVANRLVVWATYGSPRLTRLIQGTR
                     SVLVEHGRTRKQALRKELITTEELMVAIRTQGARKLRDVDLAALEPNGALTVELRDEV
                     RDEVNEVLERLRRIEAKLDAAPR"
     gene            complement(1454..>2482)
                     /locus_tag="VEP73_06090"
     CDS             complement(1454..>2482)
                     /locus_tag="VEP73_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYT26034.1"
                     /translation="GAEGIGLCRTEHMFLGEERVAAVRTMIFADTVEEEQQAYDVLLP
                     LQRDDFVGIFKEMDGKPVTVRLLDPPLHEFLPSQVDLAVAVALAKEQGAGEVRLGGED
                     PMPLEEAERVLAKVNDLHETNPMLGLRGVRLGILKPGLYAMQVRAIIEAACTVKAGGG
                     HPVVEVMIPLAATREELAQMREELEPVAKQVMEERGQELDVVFGTMIELPRAALTAGE
                     IAEVAEFFSFGTNDLSQTVFGFSRDDIGKFLGLYEERKLVPANPFVTIDRPGVGRLMQ
                     IACEEGRASRADLHLGICGEHGGDPDSVRFCHEIGLDYVSCSPFRVPTARLAAGQAAI
                     GEATSSSK"
BASE COUNT          319 a          955 c          870 g          338 t
ORIGIN      
        1 ctgctcaccc tgctggagcc gcgcaacctc actgccgagg tgctggacgg catcgccagg
       61 cactcgtgga agcacgcgcc ccccgcgacc ctcgagggga cgtgcgcccg cctggccgac
      121 cgcatcgcct acctgaccgc cgacctcacc gacgcgctcc gggccggcgt gctcggcggg
      181 atcgacgagg tgcccccgcc ggtcaggcgc gcgccctgcg cggcgtggtc gaagccgctg
      241 ggggcgaccg cctgcagggc ggcctcgccg ccatggccga agcaggcgtg cccgaggtcg
      301 tgcccggtcg cgatggcgct ggcgagcggc acgttgagcg cgagggcctg ggcgatcgtg
      361 gcggcgatcc gcgccgtgtg ctgcatgtgg gtcgccctgg tgacgaagtg gtcgccgatg
      421 taggggccgc cctgcctgcc ggccgccggc ccctccgaga aggcggggat gaacgactgc
      481 gtcttgccgt gggcccgcat catcgcccgc gagaagatga tccggtcgag gtcgcgcatc
      541 cagcaggtcc gcaccgggtc ctccggctcg ggccgtcccc ggccggccga ctcggccgcc
      601 cgggccgccc agggcgcgag cgtgcgcgac tcggccgcct ccagctcggc ccgcacggtc
      661 ccctgctgga cccagtcccg cgttcccctc accgtccgct ccttcgccgc cgccggtgcc
      721 gtccgccgtc gcgcactcga gtctgcccca gccctgtgac ggtccgcgcc gtgcatcccc
      781 accgcccgcg cggcgccgac gcagaatgga gccgcccggg cacccgcgcc caccaccatc
      841 tcgccaggag ccgcccgtgg tccacgacct gctctccctg cccgtgccgc cgctcgagaa
      901 ggtcctccgc tcggtcgccg tctacctgtt cctgctgttc gccctgcgcc tgtccggcaa
      961 gcgggaggtc gcccagctca accccttcga cttcgtcgtg ctcctgctgc tctccaacac
     1021 cgtgcagaac gcgatcatcg gcaacgacga ctcgctgctc ggcggggtgc tcggcgccgc
     1081 cgtgctgctc gtcgcgaacc gcctggtggt ctgggcgacc tacgggagcc cgcgcctgac
     1141 caggctgatc caggggacgc ggagcgtgct ggtcgagcac ggcaggacgc gcaagcaggc
     1201 gctgcgcaag gagctcatca ccaccgagga gctgatggtc gccatccgca cgcagggcgc
     1261 ccgcaagctg cgggacgtgg acctcgccgc gctcgagccg aacggggccc tgaccgtcga
     1321 gctgcgcgac gaggtgcgcg acgaggtgaa cgaggtgctc gagcgcctgc gccgcatcga
     1381 ggccaagctc gacgccgcgc cccggtgacg ccggccgggg ccaccgaggc ggccccgccg
     1441 gcgccatgcg agcctacttg gagctggacg tcgcctcgcc gatcgcggcc tggccggcgg
     1501 cgagccgggc ggtcggcacc cggaacggcg agcacgacac gtagtcgagc ccgatctcgt
     1561 ggcagaagcg caccgagtcc gggtcgccgc cgtgctcgcc acagatcccg aggtggaggt
     1621 cggcgcggga ggcccggccc tcctcgcagg cgatctgcat gagccggccg acgcccggcc
     1681 ggtcgatcgt cacgaacggg ttggcgggga cgagcttccg ctcctcgtag aggccgagga
     1741 acttgccgat gtcgtcccgg gagaacccga acacggtctg cgacaggtcg ttggtgccga
     1801 aggagaagaa ctcggccacc tcggcgatct cccccgccgt gagcgccgcg cgcggcagct
     1861 cgatcatcgt gccgaacacc acgtcgagct cctggccgcg ctcctccatg acctgcttcg
     1921 cgaccggctc gagctcctcg cgcatctggg cgagctcctc gcgcgtggcc gcgagcggga
     1981 tcatgacctc gacgaccggg tggccgccgc cggccttgac ggtgcaggcc gcctcgatga
     2041 tcgcgcggac ctgcatggcg tacagcccgg gcttgaggat gccgagccgc accccccgca
     2101 ggccgagcat cgggttcgtc tcgtgcaggt cgttgacctt cgcgagcacg cgctcggcct
     2161 cctcgagcgg catggggtcc tcgccgccga gccggacctc gcccgcgccc tgctccttgg
     2221 ccagcgcgac cgcgacggcc aggtcgacct ggctgggcag gaactcgtgc agcggcgggt
     2281 cgagcagccg cacggtgacc ggcttgccgt ccatctcctt gaagatcccg acgaagtcgt
     2341 cgcgctggag cggcagcagc acgtcgtagg cctgctgctc ctcctcgacg gtgtcggcga
     2401 agatcatcgt gcgcaccgcg gcgacccgct cctcgccgag gaacatgtgc tcggtgcggc
     2461 acaggccgat cccctcggcg cc
//
LOCUS       DASPYY010000394         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_66812, whole genome shotgun sequence.
ACCESSION   DASPYY010000394 DASPYY010000000
VERSION     DASPYY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66812"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..665
                     /locus_tag="VEP73_06095"
     CDS             3..665
                     /locus_tag="VEP73_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26035.1"
                     /translation="MDGKILLIDDEQRILRFVARGLRAEGFVVDEADSGASGLRMAPG
                     GYDVVILDLLMPGLDGATVLRRLLAQRPAQAVIVLSCLSDTATKVACLEAGAEDYLAK
                     PFSLDELLARVRARMRAAAGRMPTSLVAGRIQLDLIRREADSGAGPVPLAEREFLLLR
                     ELMQHRGRTVSKQRLLSAVWQYHFDPGSNVVDVYVRRLRAKLGAEAITTMRGEGYRIA
                     CN"
     gene            766..1800
                     /locus_tag="VEP73_06100"
     CDS             766..1800
                     /locus_tag="VEP73_06100"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HYT26036.1"
                     /translation="MPAPPAAGQEDPLPDITDQFSAPSPPRSGGRPVIGLDVGGTKIA
                     GGVVIGDGEVVDRAQVPTPDGEDGGATVDALAKVVDELRDKHPDVEAIGVGAAGLVEW
                     PSGRILWAPNNAYRDLPLRRVLHELTGLPTVVDNDATAAAWGEARFGAGAGADDMVLL
                     TVGTGVGGGLVLGGHVYRGKTGTAFEVGHMVVDPDGATCGCGKAGCLEAMASGTALGR
                     MGREAAEADPDGRLAQLAGGAEHVTGMTAFQAAREGDPVASELFRRLGFWLGVGIASL
                     VTIFDLDVVVVGGGLVATGDLLLGPARESLKRFAFAPDYRQLPPVVPARLGSEAGLVG
                     AAHLALRARG"
     gene            1886..2836
                     /locus_tag="VEP73_06105"
     CDS             1886..2836
                     /locus_tag="VEP73_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HYT26037.1"
                     /translation="MRAGATRPRLAAVVFNPTSGREPAAHREQAIRSAFDQAKMAVLW
                     LPTNATDAGAGVTGQALRAGADLVAVAGGDGTVKACAAALAGTEVPMAVLPQGTGNLL
                     ARNLGIPARLRHAVEVALAGSRRRIDVGIGDAAVNGTTSFLVMAGVGFDAAMLRDTDP
                     ELKRRLGAVAYMRSGLAELRYPQDDYTLTLDGTVRVRRRASCVLVANMGRIQGDIPVV
                     PDAVPDDGLLDVAVIRAHSLSDWLQVAARIGLRRRWGDVRVENFRIRTVEVRADAAHP
                     VEYDGDVASAPLHRLTVEVRPAALTVCVPRDAPGRPARPG"
     gene            2949..3019
                     /locus_tag="VEP73_06110"
     tRNA            2949..3019
                     /locus_tag="VEP73_06110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2981..2983,aa:Gly,seq:tcc)
     gene            complement(3049..3969)
                     /locus_tag="VEP73_06115"
     CDS             complement(3049..3969)
                     /locus_tag="VEP73_06115"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HYT26038.1"
                     /translation="MGGTEGIGHTPVVRLERLAGQGSAEVWVKLEAANPTGSYKDRMA
                     LAMIEAAERDGRLRPGQPVVEYTGGSTGSSLAFVCATKGHPLRIVSSDAFAFEKIRTM
                     QAFGADVELIPSPQGITPDLIPAMVRRAGEIAAETGAFWTDQFNNVDMLDGYRRLGEE
                     LLEQLPGPPAIEALCSYVGTAGCFLGTSRALGVKLPVLHRVVVEPAESAVLSGGAPGT
                     HHIEGGGIGRWPPLLRPEDFDEVLPVPETDAFAMVRTAARSEGIFSGPSTGANLVAAL
                     EIARRLGAGHRVVTLQVDSGLKYLAGSLYS"
BASE COUNT          521 a         1485 c         1484 g          536 t
ORIGIN      
        1 ccatggatgg caagatcctg ctcatcgacg acgagcagcg gatcctccgg ttcgtcgcca
       61 gggggctgcg cgccgagggg ttcgtcgtcg acgaggccga cagcggggcc agcggcctgc
      121 gcatggcgcc cggcgggtac gacgtcgtga tcctcgacct gctcatgcca gggctcgacg
      181 gcgcgaccgt gctgcgcagg ctcctcgccc agcggcccgc ccaggcggtc atcgtgctgt
      241 cgtgcctcag cgacaccgcc accaaggtcg cctgcctgga ggcgggcgca gaggactacc
      301 tggccaagcc gttctcgctc gacgagctgc tggcccgggt acgggcccga atgcgcgcgg
      361 ccgccgggcg catgccgacg agcctggtgg ccgggcgcat ccagctcgac ctgatccgcc
      421 gcgaggccga cagcggggcc ggccccgtgc cgctcgccga gcgggagttc ctgctgctcc
      481 gcgagctgat gcagcacagg ggcaggaccg tctccaagca gcggctgctc tcggcggtct
      541 ggcagtacca cttcgacccg ggctcgaacg tcgtcgacgt ctacgtgcgc cgcctgcgcg
      601 ccaagctcgg cgcggaggcg atcacgacca tgcgcggtga gggctatcgg atcgcctgca
      661 actgacgcga ccgggccctc cggcgagctc gtcgggcgac aggacgtgcg gtgagccgcg
      721 cccgttgcat actggaccgg cgcgatcgac cagggaggcg acacgatgcc cgctccgccc
      781 gctgccggcc aggaggaccc gctcccggac atcaccgacc agttctcggc gccctcgcca
      841 ccccgttccg ggggacggcc ggtgatcggc ctcgacgtgg ggggcacgaa gatcgcgggc
      901 ggggtggtga ttggagacgg ggaggtggtg gaccgcgcgc aggtcccgac cccggacggc
      961 gaggacggcg gcgcgaccgt ggacgccctg gccaaggtgg tcgacgagct ccgggacaag
     1021 caccccgacg tcgaggcgat cggcgtgggc gccgccggac tggtcgagtg gccctcgggc
     1081 cggatcctgt gggctccgaa caacgcctac cgggacctgc cgctgcgccg cgtgctccac
     1141 gagctgacgg gcctgcccac cgtggtcgac aacgacgcca cggcggccgc ctggggcgag
     1201 gcgcgcttcg gggccggcgc cggggccgac gacatggtgc tgctcacggt cgggaccggc
     1261 gtcggcggtg ggctcgtgct cggcgggcac gtctaccggg gcaagaccgg gaccgccttc
     1321 gaggtcggcc acatggtcgt cgacccggac ggggccacgt gcgggtgcgg caaggcaggc
     1381 tgcctggagg cgatggcgtc cggcacggcc ctcgggcgga tgggtcgcga ggcggccgag
     1441 gccgacccgg acggccggct ggcccagctg gccggcgggg ccgagcacgt gaccgggatg
     1501 acggccttcc aggcagcccg cgagggcgac ccggtcgcgt ccgagctgtt ccgccgcctg
     1561 gggttctggc tcggcgtcgg catcgcctcg ctcgtcacca tcttcgacct ggacgtggtg
     1621 gtggtcgggg gcggcctggt cgccaccggc gacctgctgc tcggacccgc gcgggagagc
     1681 ctcaagcggt tcgcgttcgc gcccgactac cgccagctcc ccccggtggt gcccgcccgg
     1741 ctgggatcgg aagccggcct ggtcggcgcc gcccacctcg ccctgcgcgc caggggctga
     1801 gcgggcgagg acgggatgag ccgacacgac cacggcgtca ggaggccgcg gtgaccggcc
     1861 ccgggcgacc gcccgcccga tagcggtgcg agccggcgcg acgcggccac gcctggccgc
     1921 cgtggtcttc aacccgacca gcgggcgcga gccggccgcg caccgcgagc aggctatccg
     1981 cagcgcgttc gaccaggcca agatggcggt cctgtggctg ccgaccaatg cgacggacgc
     2041 gggcgcgggc gtcaccggcc aggccctccg cgccggcgcc gacctcgtcg ccgtcgccgg
     2101 cggggacggg accgtcaagg cgtgcgccgc ggcgctggcc gggaccgagg tgccgatggc
     2161 cgtgctgccc caggggaccg gcaacctcct tgcccgcaac ctcggcatcc cggccaggct
     2221 gcggcacgcg gtcgaggtgg cgctggccgg cagccggcgc cggatcgacg tcggcatcgg
     2281 cgacgccgcc gtcaacggca cgacgagctt cctcgtcatg gccggcgtcg gcttcgacgc
     2341 cgccatgctg cgcgacaccg accccgagct gaagcggcgg ctcggggcgg tcgcctacat
     2401 gcgcagcggg ctcgccgagc tgcgctaccc gcaggacgac tacaccctca ccctggacgg
     2461 cacggtccgg gtgcgccgcc gggccagctg cgtgctcgtc gccaacatgg gacgcatcca
     2521 gggcgacatc cccgtcgtcc ccgacgcggt acccgacgac ggcctcctgg acgttgccgt
     2581 catccgcgcc cactcgctgt cggactggct gcaggtcgcc gcgcgcatcg ggctgcggcg
     2641 ccgctggggc gacgtgcgtg tggagaactt ccgcatccgc accgtcgagg tgcgcgccga
     2701 cgccgcccac ccggtcgagt acgacggcga cgtcgcgtcg gcgcccctcc accggctgac
     2761 ggtcgaggtc cgccccgccg ccctgaccgt ctgcgtaccc cgggacgctc ccggccgacc
     2821 cgcccggccc ggctgacccg tccgggtacg gcgtcgccgc ggagcgccgc ccgcctcata
     2881 cggctgtccc cggggacggc ggctccctgg tgatacgctc accatggccc ggccggtcgg
     2941 gtcagcaggc gggtgtagct caatggtaga gctcgagcct tccaagctcg tgacgcgggt
     3001 tcgattcccg tcacccgctc aacggtctac tcaacgtgtc gtgactcgtc agctgtacag
     3061 gctgccggcg aggtacttga ggccgctgtc gacctggagg gtgacgaccc gatggccggc
     3121 gccgaggcgg cgggcgatct cgagcgcggc gacgaggttg gccccggtcg acgggccgga
     3181 gaagatgcct tcgcttcggg cggccgttcg taccatggcg aacgcgtctg tttcgggtac
     3241 ggggaggacc tcgtcgaagt cctccggccg cagcagcggc ggccatcgcc cgatgccgcc
     3301 gccctcgatg tggtgcgtac cgggtgcgcc accggacagc accgccgatt ccgccggctc
     3361 caccaccacg cgatgcagca cagggagctt cacgcccagt gcgcggctcg tgccgaggaa
     3421 gcagcccgcc gtgccgacgt agctgcacaa cgcctcgatg gccggcggac cgggcagctg
     3481 ctcgagcagc tcctcgccga gtcgccggta gccgtcgagc atgtcgacgt tgttgaactg
     3541 gtcggtccag aacgccccgg tctccgctgc gatctcacct gcccgacgca ccatggccgg
     3601 gatcaggtcg ggcgtgatgc cctgagggct ggggatcagc tcgacgtccg cgccgaaggc
     3661 ctgcatcgtc cggatcttct cgaaggcgaa cgcgtcggac gacacgatcc gcagcggatg
     3721 ccccttcgtg gcgcagacga acgcgaggga cgagccggtg ctgccgccgg tgtactcgac
     3781 caccggctga cccggccgca gccgaccgtc ccgctcggcg gcctcgatca tggcgagcgc
     3841 catccggtct ttataggagc cggtggggtt cgccgcctcc agcttcaccc agacttcggc
     3901 gctgccctgg ccggccagcc gctccaaccg cacaacgggt gtgtggccga tgccttcggt
     3961 cccgcccatg ctgcctccta tggtctcggc ggtggtcgct tcccgaccag gagcggcgat
     4021 tcgtgg
//
LOCUS       DASPYY010000395         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1753276, whole genome shotgun sequence.
ACCESSION   DASPYY010000395 DASPYY010000000
VERSION     DASPYY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753276"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VEP73_06120"
     CDS             <1..615
                     /locus_tag="VEP73_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYT26039.1"
                     /translation="EAAPTQVPVPGTTVQVPSGLGPRTGPARAGLLLLDSDPPGARVY
                     LDGNPVGETPLPAVEAALGRHVVRMEAEGREAVSAEVELERERPLRALSFTLPVPTPA
                     GAPVRPGQLVEFSAEVTPPRRISGRVPAYPPAAQERGLEGAPVVEVWVGETGQVVDVA
                     IAESAGALLDAAVLEAVAGWQFTPARLRGVSVSVRLTFQHLFRR"
     gene            complement(682..948)
                     /locus_tag="VEP73_06125"
     CDS             complement(682..948)
                     /locus_tag="VEP73_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HYT26040.1"
                     /translation="MTYVIAEPCVNTKDTACVEVCPVDCIHPKKDEPEFEAETKLYID
                     PETCIDCGACVPVCPVQAIFPQEELPEKWAQYTQMDADWYAKRK"
     gene            complement(1036..>1534)
                     /locus_tag="VEP73_06130"
     CDS             complement(1036..>1534)
                     /locus_tag="VEP73_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26041.1"
                     /translation="RAMETAPPATSPAARLAITATVAPNTISSPPAATPAAGARPVIR
                     PPAPGPSPPARATPHEPPLEAAPPLPADGRVDRANDLLEKGRYGAALAEARAVLRRDP
                     GNAEAKMIAEDAEAATVVETRLKDARAALQRGDRDAALAEVRAGLAVRPSDARLLALF
                     RELTR"
BASE COUNT          167 a          539 c          608 g          220 t
ORIGIN      
        1 gaggccgcgc ccacgcaggt cccggtgccg ggcacgaccg tgcaggtgcc ctccgggctc
       61 ggcccgcgca cggggccggc gcgcgccggc ctccttctcc tcgacagcga cccgccgggg
      121 gcgcgcgtct atctcgacgg caacccggtg ggggagacgc cgctgccggc ggtggaagcg
      181 gccctcggcc ggcacgtcgt gcgcatggag gccgaaggcc gcgaggcggt gtcggccgag
      241 gtggagctgg agcgcgagcg gccgctgcgg gcgttgagct tcacgctccc ggtgcccacg
      301 cccgcgggag cgccggtccg gccgggtcag ctcgtggagt tctcggccga ggtcacgccg
      361 ccgcggagga tctcgggccg ggtgccggcc tatcctcccg cggcccagga gcgcggcctg
      421 gaaggcgcgc ccgtggtcga ggtctgggtc ggcgagaccg gccaggtcgt cgacgtcgcg
      481 atcgccgagt cggcgggagc cctgctggac gcggcggttc tcgaggcggt ggccggctgg
      541 cagttcacgc cggcccgcct gcgcggcgtg tccgtctccg tgcggctgac gttccagcac
      601 ctgttccggc gctgaaaccg gcggttcgag gaattacggc tgttcgggag gattccgcgt
      661 cgcgatgcgg ccggggtcgg gctacttgcg cttggcgtac cagtcggcgt ccatctgggt
      721 gtactgggcc cacttctcgg gcagctcttc ctggggaaag atcgcctgga ccgggcacac
      781 cgggacgcag gcgccgcagt cgatgcacgt ctcggggtcg atgtagagct tggtctccgc
      841 ctcgaactcc ggctcatcct tcttcggatg gatgcagtcg acgggacaaa cctccacgca
      901 cgcggtgtcc ttcgtgttga cgcagggctc tgcgatcacg taggtcatgg gacggatcct
      961 ccttggcgat ccagctcccg gcacggccgg gggctcggaa atcctactcc aaaatccgaa
     1021 ggctgtggca agccgtcacc gcgtcagctc gcgaaacagg gcgagcagcc gggcgtcgct
     1081 ggggcggacg gccagtccgg cccgcacctc cgccagcgcc gcgtcgcggt cgccgcgctg
     1141 gagggcggcg cgcgcgtcct tgaggcgcgt ctccaccacc gtcgccgcct ccgcgtcctc
     1201 ggcgatcatc ttcgcctccg cgttgccggg atcgcggcgc agcaccgccc gcgcctcggc
     1261 gagggcggcg ccgtagcggc ccttctcgag caggtcgttg gcacggtcga cgcggccgtc
     1321 ggcggggagc gggggcgcgg cctccagggg cggctcgtgc ggcgtcgccc gcgcgggcgg
     1381 gctcgggccc ggggccggcg gccggatgac cggccgggcg ccggccgcgg gtgtcgccgc
     1441 cgggggggat gaaatcgtgt tcggcgcgac ggtggccgtg atcgcgagcc gggcggcggg
     1501 ggaggtcgcg ggcggcgccg tctccatggc ccga
//
LOCUS       DASPYY010000396         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_67039, whole genome shotgun sequence.
ACCESSION   DASPYY010000396 DASPYY010000000
VERSION     DASPYY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67039"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..544
                     /locus_tag="VEP73_06135"
     CDS             107..544
                     /locus_tag="VEP73_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYT26042.1"
                     /translation="MSDRIPHAEVSERLSRAQQRYTAGRRALVELLHGAGRPVGIAEI
                     LETPSRLPQSSIYRNLVVLEQVGAVTRYPGAGGSAHYELSEELVGHHDHLVCTTCGRM
                     EDYDVPPEIEGDLLATMDAIAASKGFRATGHRLELRGRCASCA"
     gene            697..1632
                     /locus_tag="VEP73_06140"
     CDS             697..1632
                     /locus_tag="VEP73_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HYT26043.1"
                     /translation="MPKRLPPAVACVLAALVLAAGCSSGGGTSGPAAGPGGRLEVVAM
                     TTQVADFTRNVGGDRVHVTGILKPNVDPHDYEPSPADVDAIARADLVVENGVGLEHWL
                     DDVIKSSGFKGTVVDTSRGVRIRRGAGEGKAGDPHIWQDPRNAKIMAGNVEQALATAD
                     PADAATFKANLGAYAARLDALDARIAAQVRGLANKKLVTNHDAFGYYIARYGLEFVGS
                     VIPSSDTSAELSGRDVQDLVAKIRATKVKAVFSESSLPPRTAETIAREAGVKVVEGEN
                     ALYGDTLGPAGSDGDTYLKMLEHNTRTIVSNLSGV"
     gene            1643..>1962
                     /locus_tag="VEP73_06145"
     CDS             1643..>1962
                     /locus_tag="VEP73_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT26044.1"
                     /translation="MLALRHAVVAYDRAPVVEDVNGTVVPGQSVALIGPNGAGKSTLI
                     KAVLGLVPVVGGSIAVLGRPPAAARRQIAYVPQADTLDAEFPVSALQVVLMGRYPRIG
                     WVRRP"
BASE COUNT          285 a          737 c          689 g          251 t
ORIGIN      
        1 ccatggatgc gcctcggccc ggccggtcgt caccgcggcc cggcctcgtc gacgaccccc
       61 gtggcgcccg ccgtcccacg cactcccgcg cgggggtacc gccggcatga gcgaccggat
      121 cccccatgcc gaggtgtcag agcgcctcag ccgggcgcag cagcggtaca cggccgggcg
      181 ccgcgccctc gtcgagctgc tccacggggc gggccggccg gtcggcatcg ccgagatcct
      241 cgagaccccg tcgcgcctcc cgcagagctc gatctaccgc aacctcgtcg tgctcgagca
      301 ggtcggggcc gtcacgcgct acccgggagc tggcgggtcg gcccactacg agctgtcgga
      361 ggagctcgtg ggccatcacg accacctggt ctgcaccacc tgcgggcgga tggaggacta
      421 cgacgtgccg ccggagatcg agggcgacct cctggcgacc atggacgcca tcgcggccag
      481 caagggcttc cgcgcgaccg gccaccggct cgagctccgg ggccgctgcg ccagctgcgc
      541 ctgaccctcc acccactcga cctccggggc cgccctcgtg ccggcgccgt gcgcccgcgc
      601 gccagggaca gggttgtcat ccattatgat aaccattacc attacaccgt cctgtggccc
      661 gtccgtcctg ctatggagcc tgctatggag cgtcccgtgc ccaagcgtct gccgcctgcc
      721 gtggcctgcg tcctcgccgc cctggtcctc gccgccgggt gctcgagcgg cggggggacg
      781 tccgggccgg cggcgggccc gggcggcagg ctcgaggtcg tcgcgatgac cacgcaggtg
      841 gccgacttca ccaggaacgt cggcggcgac agggtgcacg tcaccggcat cctcaagccc
      901 aacgtcgacc cccacgacta cgagccctcc ccggcggacg tcgacgccat cgcacgggcc
      961 gacctggtgg tggagaacgg cgtcggcctc gagcactggc tcgacgacgt gatcaagagc
     1021 tccggcttca agggcaccgt ggtggacacg agcaggggcg tgcggatccg gcggggcgcc
     1081 ggcgagggga aggcgggcga cccccacatc tggcaggacc cgcggaacgc caagatcatg
     1141 gccgggaacg tcgagcaggc gctggccacg gcggacccgg ccgacgcggc caccttcaag
     1201 gccaacctcg gcgcctacgc ggccaggctg gacgcgctcg acgcccggat cgccgcccag
     1261 gtgcgcggcc tggcgaacaa gaagctcgtc accaaccacg acgccttcgg ctactacatc
     1321 gcccgctacg ggctcgagtt cgtcgggtcg gtcatcccga gctccgacac ctcggccgag
     1381 ctgtccggca gggacgtcca ggacctcgtc gccaagatca gggcgacgaa ggtcaaggcg
     1441 gtcttctccg agagctcgct gccgcccagg acggccgaga cgatcgccag ggaggccggg
     1501 gtcaaggtgg tggagggcga gaacgcgctc tacggcgaca ccctggggcc ggccggctcc
     1561 gacggcgaca cctacctgaa gatgctcgag cacaacaccc ggacgatcgt gagcaacctg
     1621 agtggggtct gaccgggagc cggtgctcgc cctcaggcac gcggtcgtcg cctacgaccg
     1681 cgcgccggtg gtcgaggacg tcaacggcac ggtcgtgccc ggccagtcgg tcgccctgat
     1741 cgggccgaac ggcgccggca agtccaccct catcaaggcc gtcctgggcc tggtgccggt
     1801 cgtcggcggg tcgatcgccg tgctgggccg cccgcccgca gcggccaggc ggcagatcgc
     1861 ctacgtgccc caggccgaca ccctcgacgc cgagttcccc gtctcggcac tgcaggtcgt
     1921 gctcatgggt cgctacccga ggatcgggtg ggtccgccgg cc
//
LOCUS       DASPYY010000397         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1332578, whole genome shotgun sequence.
ACCESSION   DASPYY010000397 DASPYY010000000
VERSION     DASPYY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332578"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..726
                     /locus_tag="VEP73_06150"
     CDS             79..726
                     /locus_tag="VEP73_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYT26045.1"
                     /translation="MAALGHAASPRRARARRGEGERLRTEILEAATDLLGRTGDASAV
                     SIRAIADAVGVSTPSIYLHFADKDELLWAVCEEQFARLDRTLEEAAASSSDPLESLGR
                     RGHAYVRFGLDNAEAYRILFMGRADDMPEDTAVKAVEESAAFRHLVEAVQRCVDAGAV
                     RTDDPVMVAYGLWALVHGITSLMISLPTFPWPGSPDELAERLLADHVRGLLDRGP"
     gene            780..1796
                     /locus_tag="VEP73_06155"
     CDS             780..1796
                     /locus_tag="VEP73_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYT26046.1"
                     /translation="MAAVPAADDLRLFALPGEAPPGAGGRGAPRRSGRPPRRPGPNLL
                     AVDGNGLAHRAFHAVGGAEAPPGAALERVVAMLARIAGAVRATACVVGFDDPGRSARR
                     LRAPRYKSARPPKDDALVALLDQLPALLELLGLCVVVPDGLEADDVLGSAARLATGHT
                     VAAALATGDQDAFALVSPTVQVFFLLGGGRMEQVSPSWLRARYGVGPAQYPAFAALRG
                     DSSDCLPGVRGIGAVTAARLLTAYPDVAAAMADPGGVARLLGPWVVEALVTHQDTYEL
                     NRELMAIRTDVPFDVEACNRPLHGRTVAAVLREHGIEDVADRFVAGFALLGRAAWRAS
                     APRG"
     gene            1931..>2320
                     /locus_tag="VEP73_06160"
     CDS             1931..>2320
                     /locus_tag="VEP73_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HYT26047.1"
                     /translation="MTDAAPTPTPVAPPGITLGELRASGHRRRTVKQEVRDNLLAGLA
                     VGAPRFPGIVGFDETVLPELERALIAGHDIVLLGERGQGKTRLIRSLTHLLDEWVPVV
                     AGCEINDHPDAPVCGACRARLAELGDAL"
BASE COUNT          247 a          933 c          849 g          291 t
ORIGIN      
        1 tcgccttcac gaccggatcg gcctctccga ggccggctcc gccgagccgc tgcccgagcc
       61 cgggctggcc aggctctgat ggccgccctg gggcacgccg cctccccgcg ccgggcccgc
      121 gcccggcggg gggagggcga gcgcctccgc accgagatcc tggaggccgc caccgacctg
      181 ctcggccgga ccggcgacgc gtcggcggtc tccatccgcg cgatcgccga tgccgtcggc
      241 gtcagcaccc cctcgatcta cctgcacttc gccgacaagg acgagttgct ctgggcggtc
      301 tgcgaagagc agttcgcgag gctcgaccgc acgctcgagg aggccgcggc ctcctcgagc
      361 gacccgctcg agtccctcgg gcgtcgcggt cacgcctacg tccggttcgg gctcgacaac
      421 gccgaggcct accggatcct gttcatgggc cgggccgacg acatgccgga ggacaccgcc
      481 gtcaaggccg tggaggagtc ggccgcgttc cgccacctcg tcgaggcggt ccagcgctgc
      541 gtcgacgcgg gcgcggtccg caccgacgac cccgtcatgg tggcgtacgg gctctgggcg
      601 ctggtgcacg ggatcacctc gctgatgatc tcgctgccca cctttccctg gcctggaagc
      661 cccgacgagc tcgccgagcg gctcctcgcc gaccacgtcc gcgggctgct cgaccggggc
      721 ccgtgacccc ggcctcgccg tcccttgcgc cgccgcgcgg cgaggggtat gtcttcgaca
      781 tggctgccgt gcccgccgcc gacgacctcc ggctcttcgc cctgcccggg gaggcgccgc
      841 caggcgcggg gggccggggc gcgccccgcc ggtcggggcg cccgccccgg cggccagggc
      901 cgaacctgct cgcggtcgac ggcaacgggc tcgcccaccg cgccttccat gcggtcggcg
      961 gtgccgaggc gccgccaggc gccgccctcg agcgggtcgt cgcgatgctc gcccgcatcg
     1021 cgggcgccgt ccgggccacc gcctgcgtgg tgggcttcga cgaccccggc cggtcggccc
     1081 ggcgcctgcg cgccccccgg tacaagtcgg cccggccgcc caaggacgac gcgctcgtcg
     1141 cgctgctcga ccagctcccg gccctgctcg agctgctcgg cctgtgcgtg gtggtgcccg
     1201 acgggctcga ggccgacgac gtgctcgggt cggcggcgcg gctcgccacc ggccacacgg
     1261 tggcggccgc gctggcaacc ggcgaccagg acgccttcgc gctcgtgagc cccaccgtgc
     1321 aggtcttctt cctgctcggc gggggccgca tggagcaggt gtcgccgagc tggctgcgtg
     1381 ccaggtacgg cgtcggcccc gcccagtacc ccgcgttcgc cgccctgcgc ggcgactcga
     1441 gcgactgcct gcccggggtg cgcgggatcg gcgccgtcac cgccgcccgc ctgctcaccg
     1501 cctaccccga cgtcgcggcg gccatggccg acccgggcgg ggtggccagg ctgctcggcc
     1561 cctgggtggt cgaggcgctc gtcacccacc aggacaccta cgagctgaac cgggagctga
     1621 tggcgatccg caccgacgtc cccttcgacg tggaggcatg caaccgcccc ctgcacgggc
     1681 gcacggtcgc ggccgtcctg cgcgagcacg ggatcgagga cgtcgccgac cgcttcgtcg
     1741 ccgggttcgc gctgctcggc cgggccgcct ggcgcgcgag cgccccgcgg ggctgagcgc
     1801 gcccgcggcg ctcccggacg ccgagcgttc cgcggcgctc ccggacgccc ccgtctcccg
     1861 cttgcgagcc gggccgcgga ggcggagggt ggtcggacac gccacgacga gcgaagcgag
     1921 tgatgctgcc atgaccgacg ccgcgccgac ccccacgccg gtcgcacctc ccggcatcac
     1981 gctgggcgag ctccgggcgt ccgggcaccg ccgccgcacc gtgaagcagg aggtgcgcga
     2041 caacctgctc gccgggctgg cggtgggcgc gccgcgcttc cccggcatcg tggggttcga
     2101 cgagacggtg ctcccggagc tcgagcgggc cctcatcgcc ggccacgaca tcgtgctgct
     2161 cggcgagcgc gggcagggca agacccggct gatccggtcc ctcacccacc tgctcgacga
     2221 gtgggtcccg gtcgtggccg ggtgcgagat caacgaccac cccgacgccc ccgtgtgcgg
     2281 cgcctgccgg gcgcggctcg ccgagctggg cgacgccctg
//
LOCUS       DASPYY010000398         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_910545, whole genome shotgun sequence.
ACCESSION   DASPYY010000398 DASPYY010000000
VERSION     DASPYY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910545"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VEP73_06165"
     CDS             <1..1251
                     /locus_tag="VEP73_06165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT26048.1"
                     /translation="AELADDPTFLARFRQEVGAASRVAGFCTARVLDARLEAEHPWFV
                     TEYVDGPTLSDAVARDGPLAGPRLSTFAIGVAEALDAIHAAGVVHRDLKPSNVLLARS
                     APKVIDFGIARVRDAASLTQTGKLIGTVNWLAPERFRHDRASPLSDVFAWGGLVAFAA
                     TGRPPFGSGPPEAIVHRILHEPPDLGGLDGELRGAVEAALDKDPGRRPSTRALLARLL
                     GTGVQGGEAPAAATRVVQRTWKSAPVRPVGGTPVRQAGGTPVRPGAPPAPAWPAPARN
                     GTHDLLLVLGFAGLLAATADALQPGWLHRGTVPLPLLLALGGAALLGAGRSLSGLALG
                     LLLPDLLLRNASGRPLHLAMYGLVALTMAIVGRLAGHGRRWRPLRAVAALAAGYVLLY
                     VLLRAGLAAAVVLTRDLGAAVPFP"
     gene            complement(1354..1626)
                     /locus_tag="VEP73_06170"
     CDS             complement(1354..1626)
                     /locus_tag="VEP73_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYT26049.1"
                     /translation="MIGFIVGLIIIGAIAGFLARALVPGPDPMGVAMTILLGIVGSFV
                     GGFLINLLFRGNATISPAGWIGSILGAILVLLIYRATVRRGGRSRI"
     gene            1760..2254
                     /locus_tag="VEP73_06175"
     CDS             1760..2254
                     /locus_tag="VEP73_06175"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HYT26050.1"
                     /translation="MRIETVLGDITLQEVDAIVNAANSSLLGGGGVDGAIHRAAGPEL
                     LAECRTLGGCRTGDARATKGYRLPARWVIHVVGPVWRGGGDGEAELLASCYRRGLEVA
                     DELGARSLAFPAISTGIYGYPAAEAARIAVETLHGAATDVELARLVAFDAGTLRRYAE
                     LLGS"
     gene            complement(2230..2904)
                     /locus_tag="VEP73_06180"
     CDS             complement(2230..2904)
                     /locus_tag="VEP73_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT26051.1"
                     /translation="MVVRVYVPTARTRVRRLPERGAYDRGTVHAILDEGFICHVAFTI
                     DGQPYALPTGYARVGETIYLHGSSGSRIGLQPDMPVCVTVTLLDGLVLARAAFHHSFN
                     YRSTMVLGRTRLVTNPKEKDEVLSALVEHIVPGRGADARPGDGKELAATAVLAVPLEE
                     VSAKVRSGPPKDEEEDYALPVWAGVLPLALTPGAPTPDPRLDPAVPVPSHVADWRRRQ
                     EPSSSA"
     gene            2981..>3190
                     /locus_tag="VEP73_06185"
     CDS             2981..>3190
                     /locus_tag="VEP73_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYT26052.1"
                     /translation="MNVLEQYHVTGRRSAEIAASVEAGVRDGRLGPGTTLPTVRELAA
                     GLGVSPATVAAAYAALRRRGVVTASG"
BASE COUNT          402 a         1192 c         1170 g          426 t
ORIGIN      
        1 gccgagctgg ccgacgaccc gacgttcctg gcccgcttcc gccaggaggt cggcgccgcg
       61 tcccgggtgg ccggcttctg cacggcccgg gtcctcgacg cccggctcga ggccgagcat
      121 ccatggttcg tcaccgagta cgtcgacggg ccgacgctct cggacgcggt cgcccgcgac
      181 ggtccgctgg ccggtccccg gctgtcgacg ttcgcgatcg gcgtggccga ggccctcgac
      241 gcgatccacg ccgccggggt cgtgcaccgc gacctgaagc cgagcaacgt cctgctcgcc
      301 cggtcggcgc ccaaggtgat cgacttcggg atcgcccgcg tccgggacgc ggcctcgctc
      361 acccagacgg gcaagctcat cggcacggtc aactggctcg cgccggagcg gttccgccac
      421 gaccgcgcct cgccgctgtc ggacgtcttc gcctggggcg ggctggtcgc gttcgcggcg
      481 accgggcggc ccccgttcgg cagcgggccg cccgaggcga tcgtccaccg catcctgcat
      541 gagccgcccg acctgggcgg cctcgacggg gagctccgcg gcgcggtcga ggcggccctg
      601 gacaaggacc cgggccggcg cccgtccacc cgggcgctgc tggcccgcct gctcggtacc
      661 ggcgtccagg gcggggaggc gcccgcggcg gcgacgcgcg tcgtgcagcg cacctggaag
      721 agcgcgccgg tccggccggt gggcggcacg ccggtccggc aggcgggcgg cacgccggtc
      781 cggccgggcg ccccgcccgc gccggcctgg ccggccccgg cccgcaacgg gacgcacgac
      841 ctcctgctcg tcctcgggtt cgccggcctg ctcgcggcga ccgccgacgc cctgcagcct
      901 ggctggctgc accggggtac cgtccccctg ccgctcctgc tggccttggg cggcgccgcc
      961 ctgctcggcg cgggcaggtc gctcagcggc ctcgcgctcg gcctcctgct ccccgacctg
     1021 ctcctgcgga acgcctcggg tcgacccctc cacctggcca tgtacgggct ggtcgcgctc
     1081 acgatggcga tcgtcgggcg tctcgccggg cacgggcgcc gctggcgccc gctccgggcc
     1141 gtggcggccc tcgccgccgg ctacgtcctc ctctacgtcc tcctgagggc gggcctcgcc
     1201 gccgccgtgg tcctcacccg cgacctcggg gcggccgtcc cgttcccgtg agccgttctc
     1261 gtgaagaccg aggggccgca ccgggcccgg cagctggcga caccgggcgt ggccgacggc
     1321 tcccgccggc cacgcgtcgg gggcggtacg ggttcagatc cgggagcggc cgccccggcg
     1381 cacggtcgcc cggtagatca gcagcacgag gatcgcgccg aggatcgagc cgatccagcc
     1441 ggccggcgag atggtggcgt tgcccctgaa gagcaggttg atgaggaacc cgccgacgaa
     1501 cgacccgacg atcccgagca agatcgtcat ggcgacgccc atggggtccg gcccggggac
     1561 gagcgcgcgg gccaggaacc ccgcgatcgc gccgatgatg atgagtccga cgatgaaccc
     1621 gatcatgcgc cttcctttca cgagtgagcg tggagccggt gccggctcat gtctatccag
     1681 ttggacccgc gctgaaccag gtagtttcag cgaagcgccc gattcctcgc cggtatccga
     1741 cgacaccgca ggaggggata tgcgtatcga aacggtacta ggagatataa cgctccaaga
     1801 ggtcgacgcc atcgtgaacg cggccaactc gtcgctcctc ggcggcgggg gggtcgacgg
     1861 ggcgatccac cgggcggccg gaccggagct cctggccgag tgccgcacgc tcgggggctg
     1921 ccggaccggg gacgccaggg cgaccaaggg ctaccggctg ccggcccgct gggtgatcca
     1981 cgtggtcggg ccggtgtggc gtggtggcgg cgacggcgag gcggagctgc tcgcctcctg
     2041 ctaccgccgc ggtctcgagg tggccgacga actgggcgcc cgctcgctcg ccttcccggc
     2101 catctccacg gggatctacg gctacccggc ggccgaggcg gcgcggatcg cggtcgagac
     2161 gctgcacggg gcggccaccg acgtcgagct ggccaggctg gtggccttcg atgccgggac
     2221 cctgcgccgc tacgccgagc tgctcggctc ctgacggcgg cgccagtccg ccacgtggga
     2281 cggcaccggc acggccgggt cgagccgcgg gtcgggcgtc ggcgcgccgg gcgtgagcgc
     2341 gagcggcagc acgcccgccc acaccggcag ggcgtagtcc tcctcctcgt ccttcggcgg
     2401 gccgctacgg accttggccg agacctcttc cagcggcacg gcgagtaccg cagtcgcggc
     2461 gagctccttg ccgtcaccag gccgggcatc cgccccgcgg cccggaacga tgtgctcgac
     2521 caatgcgctc agcacttcgt ccttctcctt gggattcgtg acaaggcggg tacggccaag
     2581 caccatcgtg gacctgtaat tgaaggagtg gtggaacgcg gccctggcca agaccaagcc
     2641 atcgagcagg gtgacggtga cgcacaccgg catgtccggc tgtaggccga tgcggctgcc
     2701 gctggacccg tgcaggtaga tggtctcccc tacccgtgcg tagccggtgg ggagcgcgta
     2761 cggctggccg tcgatggtga aggcgacatg gcagatgaag ccctcgtcga ggatcgcgtg
     2821 cacggtcccc cggtcgtagg caccccgctc gggcagccgc cgcaccctgg tcctggccgt
     2881 cggaacgtac acgcgcacca ccatccccca caggatccgt ccccccgcag gatccgtccc
     2941 ccgcagaact tctccggtgg gattgttata gtacataccg atgaacgtac tggaacaata
     3001 tcacgtcacc gggcggcggt cagccgagat cgccgcgagc gtcgaggccg gcgtgcggga
     3061 cggccggctc ggcccgggca cgaccctgcc gacggtcagg gagctggccg ccggcctggg
     3121 agtgagcccg gcgaccgtcg ccgcggccta cgccgcgctg cggcgccgcg gcgtggtgac
     3181 ggccagcggg
//
LOCUS       DASPYY010000399         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_67390, whole genome shotgun sequence.
ACCESSION   DASPYY010000399 DASPYY010000000
VERSION     DASPYY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67390"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEP73_06190"
     CDS             complement(<1..224)
                     /locus_tag="VEP73_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HYT26053.1"
                     /translation="MFDTLSDRLQDVFTKLRGHGRLSEAQVDAALREVRLALLEADVN
                     FKVVKDFIGRVRARAVGEEVTRSLTPAQQV"
     gene            458..>1858
                     /locus_tag="VEP73_06195"
     CDS             458..>1858
                     /locus_tag="VEP73_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYT26054.1"
                     /translation="MARTPGRVSASTKRAGALVVGRWVGVSVVSALLALQLARKPSAL
                     LLAALVSAVPVAGANLAQQRALAAGRTAAVRGWGRALLALDGVFVLLTVLGMSRQPVG
                     GYFMLGWLPIEGALAEGGAAAVAGGALAVAAVLAEAAMRRRGLPLPSMYDGSAVRILN
                     LVAITGVMVAVERALGRGERAMAQRTAELADAVEREREARLELQAFSTIVLAGVADQG
                     GLDEVLESMTRAVARTMDYEAMAVYLLDRPGLLHCRSWFGDWPRQAAPIFVVGAGSIV
                     GRVAEQGRGILLGDPEEHPDYFAAAPGPRSEICAPLRIGDRVLGVVNVEASVPHRYGQ
                     ADLDRLQRLADQLAVVVERTRLADREAATVARLHELDRLKDDFLAMTSHELRTPLTVI
                     RGFTSTLLRGDQELGAEERRRFTEVIDRQTARLTRLVEDLLAASRMEAGKLDIVMGEH
                     EPAKLLTEFAEEWPTGP"
BASE COUNT          224 a          633 c          746 g          255 t
ORIGIN      
        1 atcacctgct gggcgggggt gaggctgcgg gtcacctcct cgccgacggc gcgggcgcgc
       61 acccggccga tgaagtcctt gacgactttg aagttgacgt ccgcctccag cagggcgagg
      121 cggacctcgc gcagggcggc gtcgacctgc gcctctgaga gccgcccgtg cccccggagc
      181 ttggtgaaga cgtcctggag acggtcggag agcgtgtcga acacgggtct gcgtcccttt
      241 gccttggaag ccggtgcctt ggaagcggcc ggtcggtgcc tcaagcatag tggtcccgca
      301 ggcgctcgtt cgcccgtcca caggcaccgc ccccggggaa ggcaggcagc cgcgatggtg
      361 atagcgtttc gcggcgcact cccagcagcg gccaaggacc ggcgggaccg ctcggcctgc
      421 caggacgggc ccggggacgc gatcgtggag ggggcggatg gcgcgaacgc cgggaagggt
      481 ctcggcctcg acgaaacggg cgggcgcgct cgtggtcggc cgctgggtcg gggtctcggt
      541 cgtctcggcg ctgctcgcgc tccagctcgc ccgcaagccg agcgcgctgc tgctggcggc
      601 gctcgtcagc gccgtgccgg tggccggtgc gaacctggcg cagcagcggg cgctcgccgc
      661 gggacggacg gcggcggtgc gtggctgggg ccgtgcgctg ctcgcgctcg acggggtgtt
      721 cgtgctgctg accgtcctcg ggatgagcag gcagccggtc ggtgggtact tcatgctcgg
      781 ctggctcccg atcgaaggcg cgctcgccga gggcggggcc gccgcggtcg ccggcggcgc
      841 gctcgcggtc gcggcggtgc tcgccgaggc cgccatgcgg cggcgcgggc tccccctccc
      901 gtccatgtac gacggctcgg ccgtgcgcat cctcaacctg gtggcgatca ccggggtgat
      961 ggtcgcggtg gagcgggcgc tcgggcgcgg cgagcgggcg atggcccagc gcaccgccga
     1021 gctcgcggac gcggtcgagc gcgagcgcga ggcgcggctc gagctgcagg cgttctcgac
     1081 gatcgtgctg gccggcgtgg ccgaccaggg cggtctcgac gaggtgctcg agtcgatgac
     1141 cagggcggtc gccaggacga tggactacga ggcgatggcg gtgtacctgc tcgaccggcc
     1201 cggcctgttg cactgccgct cctggttcgg cgactggccg cggcaggcgg cgccgatctt
     1261 cgtggtgggc gcgggcagca tcgtcggccg ggtcgccgag caggggcggg ggatcctgct
     1321 cggcgacccg gaggagcacc cggactactt cgcggccgcc cccggcccca ggtcggagat
     1381 ctgcgcgccc ctgcggatcg gcgatcgggt cctcggcgtg gtcaacgtgg aggcgtcggt
     1441 gccgcaccgg tacggccagg ccgaccttga ccggttgcag cggctcgccg accagctcgc
     1501 cgtggtcgtc gagcggacgc ggctggccga ccgggaggcg gccacggtcg cgcgcctgca
     1561 cgagctcgac cggctgaagg acgacttcct ggcgatgacc tcacacgagc tgcggacgcc
     1621 gctgacggtg atccgcgggt tcaccagcac gctgctgcgt ggcgaccagg agctcggggc
     1681 cgaggagcgg cggaggttca ccgaggtcat cgaccggcag accgcgcgcc tgacccggct
     1741 ggtcgaggac ctcctggccg cgtcccgcat ggaggcgggc aagctcgaca tcgtcatggg
     1801 cgagcacgag ccggccaagc tcctgaccga gttcgccgag gagtggccga ccgggccg
//
LOCUS       DASPYY010000400         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_209208, whole genome shotgun sequence.
ACCESSION   DASPYY010000400 DASPYY010000000
VERSION     DASPYY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209208"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1550)
                     /locus_tag="VEP73_06200"
     CDS             complement(<1..>1550)
                     /locus_tag="VEP73_06200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT26055.1"
                     /translation="ADPGRAGPGPAEGPGALGPEALPPAPAAADGGPGRPGPGVDPPG
                     PDVAPVLRGLDPDQRAAAMVTDGPLLIVAGPGTGKTRTLTHRIAHLVTGQGVPPERCL
                     AITFTRRACEELHERLRALLPDAADRVLVATFHGLGLRIVREQHRALGLGVRVGIADE
                     AERLALVAELAGGSERDARRLSGALSRAKRARAAGPEPVAGELAGPLARYDETLRARD
                     LVDFDDLLALPVTLLGADPGLAASYRDRWQRISVDEYQDVDELQYRLLRLLAPPGGHL
                     CAIGDPDQAIYGFRGSHVGFFLRFTDDFPAARTVRLTRNYRSSRAIVDGALQAVAPST
                     LVRGRVLDPQRREPSPPRIVVHQAATEQGEADFVVRTVDELLGGSSLYSIDSGRVGPG
                     VDGHGGLSFADVAVLYRTDAQAGPLADALARAGMPFQKRSHDRLTARPGVRAVVGRLR
                     AARAAGEPAGGEEPPVLTRVHRAVEAALDDARAGPAARGERLDRAELVLSAVAEDELR
                     HALDLLTPL"
BASE COUNT          172 a          589 c          639 g          150 t
ORIGIN      
        1 agcggcgtga gcaggtccag cgcatgccgc agctcgtcct cggccacggc cgacaggacc
       61 agctcggccc ggtccagccg ctcgccgcgc gcggccgggc cggcccgtgc gtcgtcgagc
      121 gccgcctcga cggcgcggtg cacgcgggtg agcaccggcg gctcctcccc gccggccggc
      181 tcgcccgccg cgcgcgcggc gcgcaggcgc ccgacgacgg cccggacgcc gggccgcgcg
      241 gtgaggcggt cgtgggagcg cttctggaat ggcatgccgg cacgcgcgag ggcgtcggcg
      301 agcgggccgg cctgggcgtc ggtgcggtag aggacggcga cgtcggcgaa cgagaggccg
      361 ccatggccgt ccacgccggg gcccacgcgg ccgctgtcga tcgagtacag cgaggacccg
      421 ccgagcagct cgtccacggt ccgcacgacg aagtcggcct cgccctgctc ggtcgcggcc
      481 tggtggacga cgatccgcgg gggcgacggc tcccgccgct gcgggtcgag cacccggccg
      541 cgcaccagcg tcgacggggc gacggcctgg agcgcgccgt ccacgatcgc gcggctcgag
      601 cggtagttgc gcgtgaggcg gacggtccgc gccgccggga agtcgtccgt gaagcgcagg
      661 aagaagccga cgtgcgagcc gcggaagccg tagatcgcct ggtcggggtc gccgatggcg
      721 cagaggtggc cgcccggcgg ggcgagcagg cgcagcaggc ggtactgcag ctcgtcgacg
      781 tcctggtact cgtcgaccga gatccgctgc cagcggtccc ggtagctcgc cgcgagcccg
      841 gggtcggcgc cgagcagcgt gaccggcagc gcgaggaggt cgtcgaagtc gaccaggtcc
      901 cgtgcccgca gggtctcgtc gtagcgcgcg agcggcccgg ccagctcgcc ggcgaccggc
      961 tccgggccgg cggcccgcgc ccgcttggcc cgcgagagcg ccccggagag gcggcgcgcg
     1021 tcgcgctccg agccgcccgc gagctcggcg acgagcgcca gccgctcggc ctcgtcggcg
     1081 atgccgacgc gcacgccgag cccgagcgcc cggtgctgct cgcggacgat ccgcagcccg
     1141 agcccgtgga aggtggccac gaggacccgg tcggccgcgt cgggcagcag cgcgcggagc
     1201 cgctcgtgca gctcctcgca cgcccgccgg gtgaaggtga tggcgaggca gcgctcgggc
     1261 ggcacgccct gcccggtcac gaggtgggcg atccggtggg tgagcgtgcg cgtcttgccg
     1321 gtgcccggcc cggcgacgat gagcagcgga ccgtcggtca ccatggccgc ggcccgctgg
     1381 tccgggtcga gcccccgcag gaccggggcg acgtccgggc cgggcgggtc gaccccgggc
     1441 ccaggccggc ccgggccgcc gtccgccgcg gcaggcgcgg gcgggagggc ctccggcccg
     1501 agcgccccgg gaccctccgc cgggcccggg ccggcgcgtc cggggtccgc
//
LOCUS       DASPYY010000401         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1896506, whole genome shotgun sequence.
ACCESSION   DASPYY010000401 DASPYY010000000
VERSION     DASPYY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896506"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..838)
                     /locus_tag="VEP73_06205"
     CDS             complement(449..838)
                     /locus_tag="VEP73_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26056.1"
                     /translation="MHIKRFGTTLDGRVFQTARGGILQDSGYNEVWDQARREALTPAQ
                     YRSPLGRRPYDLRHAAVSLWLNSGVPATEVARRAGHGVAVLLKIYAHCIDGQATAANQ
                     RIAEALGIQDAEEDLGDEGDGDIEQAS"
     gene            complement(936..1865)
                     /locus_tag="VEP73_06210"
     CDS             complement(936..1865)
                     /locus_tag="VEP73_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26057.1"
                     /translation="MDSYDVRFWDTKKIADNASGGRYRVRWAVNGQEHCKSFKNKTLA
                     DGFLDNLKDAARDRRPFSPRTGLPAAETTDGEMVTWYEHARSYAEAKWPSLAPVSRRS
                     VAEALVTVTVALTRKEKGAPEPRVLRRALFAWAFNPATREQVPPREIALALGWAARAS
                     LPVAALDDPATVRVALGACAKTLTGKPAAGSTQRRKRSVFYNALGYAVELGRLGSNPV
                     DRIQWTAPAVAASVDRRVVVSPAQAGTLLTAVGRLGKRGAHLKAFFATLYYAALRPSE
                     AVMLRQADLHLPKTRVGTDRPFGLGVAGRAGLD"
     gene            complement(1915..2238)
                     /locus_tag="VEP73_06215"
                     /pseudo
     CDS             complement(1915..2238)
                     /locus_tag="VEP73_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          284 a          791 c          875 g          343 t
ORIGIN      
        1 tgaacttgcc ctcggatggt aacccaacgc gcgcccgttt gttgtccttt ccgcgccgag
       61 atgtcggcgt ccaggtgtct gtccatttat ggcaagtggc ggcatggtat gggagaggct
      121 gcataggaga aagaaagacg cggtgccgcg cctccgttcg aacaggtgca cgcaaagccg
      181 acggttttac agcccgctcg ctccgcccga gtcacccccc gctgaccagc gcatacgccg
      241 ttcgaggcgc gtttgcgggc cgccgtcgtc cgctatacgt ccgtgggtgc cgggttccgg
      301 ggtccgtgcg gcccacggac gggcgcggac cagcccacgg acgggcacgg accagcccac
      361 ggacgaggac ggaaaaggcc acggacgggg ccggtgggag cggttatgca gaccgtcccc
      421 gcccggattc ccgccctgac ctgccatttc aggatgcctg ctcgatgtcg ccgtcgccct
      481 cgtcaccgag gtcttcctcg gcatcctgga tgccgagggc ctcggcgatg cgctgattgg
      541 cggcggtcgc ctggccgtcg atgcagtggg cgtagatctt cagcaggacc gcgacgccgt
      601 gcccggcgcg gcgcgcgacc tcggtcgcgg gcaccccgga gttcagccac agcgacaccg
      661 ccgcgtgccg caggtcgtac gggcggcggc ccagcggtga ccggtactgg gcgggggtga
      721 gcgcttccct gcgggcctgg tcccagacct cgttgtagcc ggaatcctgc aagatgccgc
      781 cccgggcggt ctggaagacc cgcccgtcga gggtcgtgcc gaaccgcttg atgtgcacgc
      841 ggagcagccc gaccaggacc ggcgggatgg ggatggtgcg ggtctcgttg tcggcgcggt
      901 gcttcagacc gcgttcctgc cgtgcggttc ccgcgtcagt ccaggcccgc ccggcccgcg
      961 acgccgaggc cgaaaggacg atccgtcccc acccgggtct tgggcaggtg caggtcggcc
     1021 tggcggagca tgaccgcctc cgacgggcgc agcgccgcgt agtaaagggt ggcgaagaac
     1081 gccttcaggt gcgctccccg cttccccagc cgcccgacgg cggtcaggag cgtcccggcc
     1141 tgggcggggc tgacgacgac ccgccggtcc acgctcgccg cgacggcggg ggcggtccac
     1201 tggatccggt cgaccgggtt cgagccgagg cggccgagtt cgacggcgta gccgagggcg
     1261 ttgtagaaca ccgaccgctt gcgccgctgg gtcgacccgg cggccggctt tccggtcagg
     1321 gtcttggcgc acgcgccgag cgcgacccgc acggtggccg ggtcgtccag cgcggcgacg
     1381 ggcagcgagg cgcgggccgc ccagccgagg gcgagggcga tctcgcgcgg cgggacctgc
     1441 tcgcgggtgg cggggttgaa cgcccaggcg aacagcgccc ggcgcagcac cctgggctcg
     1501 ggcgcgccct tctctttcct ggtgagggcg acggtcacgg tgaccagggc ctcggcgacc
     1561 gaccggcggg agaccggggc gaggctaggc catttggcct cggcgtagct gcgggcgtgc
     1621 tcgtaccagg taaccatctc gccgtcggtg gtctcggcgg cgggcaggcc ggtgcggggg
     1681 ctgaagggcc gacggtcgcg ggcggcgtcc ttgaggttgt cgaggaaccc gtcggcgagg
     1741 gtcttgttct tgaacgactt gcaatgctcc tggccgttga cggcccatcg gacccggtag
     1801 cgtccgccgc tggcgttgtc ggcgatcttc ttggtgtccc agaacctgac gtcgtaactg
     1861 tccatctgct ccttgctcct ttgcggtcgt ggcttcttcc aggcggcgca gccactggct
     1921 gagtgcgctg cgccggaccc gcaccccgcc gccgggcagc cgcaccgctg ccgggccggc
     1981 tcccagccgg cgccagcggt agaacgcggc ccgggatacc cgcagctcgg cggtcacctc
     2041 ctcgatggtc agcagcgggt cgcccagggt tccgggggct ttgtccgggg tggcgggggg
     2101 ccggccggga gcggttggcg ttcccggggg cggcccgtcc gtgggtccgc gacgacggac
     2161 gctccgctcc gcccggcctg tcaagtccgg cccccgccgg gccggcgacg gcgggcggct
     2221 gcccggggct gccgacactg ggaccagggg aaagaggtaa ggccccgcgg tcgcgctgtg
     2281 cggcggggcg ggt
//
LOCUS       DASPYY010000402         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2035392, whole genome shotgun sequence.
ACCESSION   DASPYY010000402 DASPYY010000000
VERSION     DASPYY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035392"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(743..1576)
                     /locus_tag="VEP73_06220"
     CDS             complement(743..1576)
                     /locus_tag="VEP73_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT26058.1"
                     /translation="MRVPAFICRTCGVEYPAASAPPASCPICEDERQYVGHGGQRWTT
                     LAELAGEGHRSDLREVEPALLGIGVTPRFAIGHRGLLVQTAAGNLLWDQPGFFDDAAV
                     DAVRDRGGVAAIAASHPHFYGSMPSWSRRFGDAPILVPAADRGWLPYEGKAVTRWSGT
                     TEILPGVTLVQCGGHFPGSTAVHWAAGAGDRGALLTGDTIAVAADRRWVSFMRSYPNN
                     IPLPGAAIRRILDRLAPYRYDRIYGGWWDSVVAEDAGSAVERSADRYVRWIVGEEDPD
                     R"
     gene            complement(1601..2647)
                     /locus_tag="VEP73_06225"
     CDS             complement(1601..2647)
                     /locus_tag="VEP73_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HYT26059.1"
                     /translation="MDMPHATGDEQERLPVVDVGPLADQTGGQPEVAARLAHACRRCG
                     FFYVTGHGVDEGLQRRLEQLSRAFFAQEESAKLEIRLALGGRALRGYFPVGAELTSGR
                     PDRKEGIYFGTELCPDDPRVLAGTPLHGSNLFPAGSPELRDTVLAYMAAMTRLGHTLM
                     AGIAQSLGLEASYFADRYTADPLTLFRIFNYPPDPSPAGQEESWGVGEHTDYGLLTIL
                     KQDDSGGLEVRSGGRWIAAPPVAGSFVCNIGDMLDRMTGGRYRSTPHRVRNQARHDRL
                     SFPFFFDPSFDARVEPIPRDSSSPAGDGSGAPTVADQRWDRASVHEFQGTYGDYVLGK
                     VARVFPELGIGATP"
     gene            complement(2765..>3154)
                     /locus_tag="VEP73_06230"
     CDS             complement(2765..>3154)
                     /locus_tag="VEP73_06230"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HYT26060.1"
                     /translation="RLPYFWARMSVRRDGGRVRYRSHRRWPGPGGACCLADVEVGTAL
                     GSGELGALDHFLTARYRLFTVVAGRLAQAEAEHPPWPLARARLVRLDQDLVEAAGLPR
                     PERTPLVHHSPGVEVRVGRWRFARGTA"
BASE COUNT          440 a         1148 c         1176 g          390 t
ORIGIN      
        1 acggtcgaac tggtgttcgc atccatggcg agaccctagc ctggccggcg cccgccgccc
       61 agcccaccac catcatctgt ggacgacgag accacggagg acgggcaggt cacccggcac
      121 agatccggtg cggccaccac cagggaaggg cgtgtccgac agagcctcgc ctggaccgca
      181 cgccccggaa gaccggctgc gaccggtttg tgcgttgacg tggggacggc acctgggacc
      241 ggctgccggc gcctgcgcag actcggtcgg acgggtgggt gaggggagct gtcgagcgtc
      301 accctcgcct cgcgccaacg tcatgacgac acccagcccg ggtcagtgct ggaggcgatt
      361 acggggccac gaccggcagg cgagggcagc cccgcgatcg gaccggtcag cgatcggtcg
      421 gcgacacgac ggcggccgcc tcgaccagtc gggagcggca gctgccaagg ccaaccgtag
      481 gggcgccacc ggagagccga cgactgcgaa cggtccaggc tgagcgcgtc ggcgagctcg
      541 gccagatcgt gcgcgacgcc cagcgggtgg ccgccgggtg cgggccagag cgcccgtagg
      601 cgggccgatc gagcgtgaga cgtaacccgc tccttgagtg gtccctccat ggccaccgag
      661 acgccccacc acctgaaccc gccgcagggt acctggtcgc ggctgagctt gcgttcacca
      721 tcaccgcggc gtcctccgcg gttcaccggt cggggtcctc ctcgccgacg atccagcgga
      781 cgtagcggtc ggcggaccgc tccacggcgg acccggcgtc ctcggcgacg accgagtccc
      841 accagccgcc gtagatgcgg tcatagcggt acggagcgag ccggtcgagg atccgccgga
      901 tggcagcccc tggcagcggg atgttgttcg gatagctgcg catgaacgag acccagcggc
      961 ggtcggcggc gaccgcgatc gtgtcgccgg tgagcagtgc cccccggtcc cccgcgccag
     1021 ccgcccagtg caccgcggtg ctgcccggga agtgcccgcc gcactgcacc agggtgaccc
     1081 cgggcaggat ctcggtggtg ccgctccacc tggtcaccgc cttgccctcg tagggcagcc
     1141 agccccggtc ggccgcgggg acgaggatgg gtgcgtcgcc gaagcgccgg ctccagctcg
     1201 gcatggaccc gtagaagtgg gggtgcgagg ccgcgatggc agcgacgccg ccgcggtcgc
     1261 gcacggcgtc gacggccgcg tcgtcgaaga agccgggctg gtcccacagc aggttccccg
     1321 ccgcggtctg cacgagcagg ccgcgatggc cgatggcgaa cctcggcgtc acaccgatgc
     1381 cgagcagggc cggctcgacc tcgcggaggt cgctgcggtg gccttcgccg gcgagctcgg
     1441 ccagggtggt ccaccgctgc ccgccgtggc cgacgtactg gcgctcgtcc tcgcagatcg
     1501 ggcaggacgc gggtggggcg gaggcggcag ggtactcgac cccgcaggtc cggcagatga
     1561 acgcgggcac gcgcatggcg gacctcctcg cggtcggtcg ttacggcgtc gccccgatgc
     1621 cgagctccgg gaagaccctg gccaccttgc cgaggacgta gtcgccgtag gtgccctgga
     1681 actcgtgcac gctggcccgg tcccaccgct ggtcggcgac cgtcggcgcg cccgacccgt
     1741 cgccggccgg cgacgaggag tcccggggga tcggctccac gcgggcgtcg aagctcgggt
     1801 cgaagaagaa cgggaacgag agccggtcat gccgggcctg gttgcgcacc cggtgcgggg
     1861 tggaccggta cctgcccccg gtcatccggt cgagcatgtc gccgatgttg cacacgaacg
     1921 agcccgcgac cgggggcgcg gcgatccagc gcccccccga ccgcacctcc agcccgccgg
     1981 agtcgtcctg cttcaggatc gtcagcaggc cgtagtcggt gtgctcgccg accccccagg
     2041 actcctcttg gccggccggc gaagggtcgg gcgggtagtt gaagatgcgg aacagcgtca
     2101 gcgggtccgc ggtgtagcga tcggcgaagt aggacgcctc caggccaagg ctctgcgcga
     2161 tcccggccat cagcgtgtgc cccagccgcg tcatcgccgc catgtacgcg agcacggtgt
     2221 cgcggagctc ggggctgccc gcggggaaca ggttcgagcc gtgcaagggg gtgcccgcca
     2281 gcacgcgggg gtcgtcgggg cacagctcgg tgccgaagta gattccctcc ttgcggtcgg
     2341 gccggcccga ggtcagctcg gcgccgacgg ggaagtaccc ccgcagggcc ctgccgccga
     2401 gagccagccg gatctcgagc ttggcgctct cctcctgggc gaagaacgcc cggctgagct
     2461 gctccagccg ccgttgcagc ccctcgtcga cgccgtggcc ggtgacgtag aagaacccgc
     2521 accggcggca ggcgtgggcg agccgcgcgg cgacctccgg ctgcccgccg gtctggtcgg
     2581 ccaacgggcc gacgtcgacg accggtaggc gctcctgctc gtcgccggtg gcgtgcggca
     2641 tatccatggc ggacctcctc gaacggccgg gacttgcccc gggtggcagg ctctgcctcg
     2701 tcgcccggtg gtcgcccggt gatggtaccg acgcccgggt catgccgtct tccgatgccc
     2761 cgggtcatgc cgtcccccgc gcgaacctcc agcgccccac gcggacctcg acgccgggcg
     2821 agtggtggac cagcggcgtc cgctccggcc ggggaagccc ggccgcctcc accaggtcct
     2881 ggtccaggcg gacgagccgg gcgcgagcca ggggccacgg cgggtgctcg gcctcggcct
     2941 gggcgaggcg gccggcgacc acggtgaaca ggcggtaccg ggcggtgagg aagtggtcga
     3001 gcgcccccag ctccccggag cccagggcgg tgccgacctc gacgtcagcc aggcagcagg
     3061 cgcctccggg ccccggccag cggcgatggc tccggtagcg cacgcggccg ccgtcccgcc
     3121 gcaccgacat ccgcgcccag aagtacggca gccg
//
LOCUS       DASPYY010000403         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1754239, whole genome shotgun sequence.
ACCESSION   DASPYY010000403 DASPYY010000000
VERSION     DASPYY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754239"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VEP73_06235"
     CDS             complement(<1..557)
                     /locus_tag="VEP73_06235"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYT26061.1"
                     /translation="MTDRAPAGPAAAHWLRLAAAVATGLGLALAFPGPDLGAVAFVAL
                     VPLLVAVEPVRPRVAAGLGLVAGLTFFGLHLHWIDVFGNLAWLALSVAQALFVAAFFA
                     LVPATRRLGTWRVALLPACWAALELLRAHVPLGGFPWGMLGLTQHDGGPLLPLARVVG
                     GYGLSAVVVAVNLALAAALRALLAA"
     gene            complement(554..826)
                     /locus_tag="VEP73_06240"
     CDS             complement(554..826)
                     /locus_tag="VEP73_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HYT26062.1"
                     /translation="MKVPEEEEEEAQVEASLEELIAKKADRPSAGEEEDDDESLLTLG
                     RDEARESLSTKVLPVQQNEFVCKSCFLVKHRSQLADRRRELCRDCA"
     gene            complement(1122..>1877)
                     /locus_tag="VEP73_06245"
     CDS             complement(1122..>1877)
                     /locus_tag="VEP73_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26063.1"
                     /translation="KEALARADEADGRAAALDAEAVGLGDAEARLAAARAAEREASEA
                     AGRAHGERRRATEALAGAGRRHGDQARALADGRERHEAVAALEEEAGYLARVVDLVVA
                     FRLHLVSRLGRRLSAEAAGLFAELTDNEYQDLVVDPEDYSVRIADAGVEYELARYSGS
                     ENDLASLSLRVAVSLVIAEGAGELGVLVLDEVLGALDRERRERMLAALTTLQGRFRQV
                     LLVTHNDEVKDLLPAAIEVRRGPYRTSTATVLG"
BASE COUNT          236 a          717 c          702 g          222 t
ORIGIN      
        1 caggccgcga gcagcgcccg cagggcggcc gccagcgcca ggttgaccgc cacgaccacc
       61 gcggacaggc cgtagccgcc gaccacccgg gcgagcggca gcagcggccc accgtcgtgc
      121 tgggtgagcc cgagcattcc ccacgggaag ccgccgagcg ggacgtgggc gcggagcagc
      181 tcgagcgccg cccagcaggc gggcaggagc gccacccgcc aggtcccgag ccgcctggtc
      241 gccgggacca gggcgaagaa ggcggcgacg aacagcgcct gcgcgacgct gagcgccagc
      301 cacgcgaggt tgccgaagac gtcgatccag tggaggtgca ggccgaagaa ggtcaggccg
      361 gcgaccaggc cgaggccggc agccacccgg ggccgcaccg gctcgaccgc gacgagcagc
      421 gggaccaggg cgacgaacgc gaccgcgccg aggtcgggcc ctggaaaggc gagcgccagg
      481 ccgagcccgg tcgcgaccgc ggcggccagg cgcagccagt gggccgcggc cgggcccgcc
      541 ggggcgcggt cggtcacgcg cagtcgcggc acagctcgcg ccggcggtcg gcgagctggc
      601 tccggtgctt caccaggaag cagctcttgc agacgaactc gttctgctgg accggcagca
      661 ccttggtgct cagcgactcg cgggcctcgt cgcgcccgag cgtcagcagc gactcgtcgt
      721 cgtcctcctc ctcgccggcc gacggccggt cggccttctt cgcgatcagt tcctcgagcg
      781 aggcttcgac ctgcgcctct tcttcctctt cctcaggcac cttcatgatt cctttccatc
      841 cgctgcgccg acgcctcgcg ccggtggtct cgtctgtcac ggcctggccg gtttggaccg
      901 tgcagtcctc cgtcaacagc tctggaccgg tcgttgttcc gcagggcgac gacatgatgc
      961 accaggcccg caagcggtcg tgggcgagac ggggagcggg cggggcggcg aggcccggcc
     1021 ccagggtcgg cgcagtatcg gcccgggtcg cgggccacgt caatggcgct ggcgcggccc
     1081 gcgacgggtg ccgttgtcct ggcgcagccg gccggccgcc ttcagccgag cactgtggcc
     1141 gtcgaggtcc tgtacgggcc cctgcgcacc tcgatcgcgg ccgggagcag gtccttgacc
     1201 tcgtcgttgt gggtgaccag cagcacctgc ctgaagcggc cctgcagggt cgtgagcgcc
     1261 gcgagcatcc gctcgcgccg ctcccggtcg agcgcgccga gcacctcgtc gaggacgagc
     1321 acgccgagct ctccggcgcc ctcggcgatc accaggctga ccgccacccg gagcgagagg
     1381 ctcgcgaggt cgttctcgga ccctgagtag cgcgccagct cgtactccac cccggcgtcg
     1441 gcgatgcgga cggagtagtc ctccgggtcg acgaccaggt cctggtactc gttgtcggtc
     1501 agctcggcga acaggccggc cgcctcggcc gagagccgcc ggccgaggcg ggagacgagg
     1561 tgcagccgga acgcgacgac aaggtcgacg acccgcgcga ggtagccggc ctcctcctcg
     1621 agcgcggcga cggcctcgtg ccgctcccgg ccgtcggcga gggcccgcgc ctggtcgccg
     1681 tgccgccggc cggcgcccgc gagcgcctcg gtggcccgcc ggcgctcccc gtgcgcccgg
     1741 ccggcggcct cggacgcctc ccgctcggcg gcgcgggcgg cggccaggcg cgcctcggcg
     1801 tcgccgagcc cgacggcctc ggcgtcgagc gcggcggccc gcccgtcggc ctcgtcggcc
     1861 cgcgcgaggg cctcctt
//
LOCUS       DASPYY010000404         7563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_67989, whole genome shotgun sequence.
ACCESSION   DASPYY010000404 DASPYY010000000
VERSION     DASPYY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67989"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..855
                     /locus_tag="VEP73_06250"
     CDS             409..855
                     /locus_tag="VEP73_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYT26064.1"
                     /translation="MGAPSGPAVAVGLVARDPRGRLLLVRRGHPPARGAWSLPGGRVE
                     AGETMAEAAARELREETGLTARVGAVAGVVERIAEGYHYVIVDLWAELDGAEPVAGDD
                     ATAARLVEPSELPALDLSPGLGEFLAGIGCWPDGAPPPEPYQPEPS"
     gene            939..1730
                     /gene="mutM"
                     /locus_tag="VEP73_06255"
     CDS             939..1730
                     /gene="mutM"
                     /locus_tag="VEP73_06255"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYT26065.1"
                     /translation="MPELPEVESVRRQLEPELRGRRVLRVRADAQRRFHAVERLAGRR
                     VEAVGRRGKFILCPLDAGLELVLHLGMTGSLHFGPADAYARALLALDDGRELVFRDVR
                     RFGCVAVVEAGAYGPLPTLARLGPEPLSDDFDPDRFARDLAVTRAPVKPYLLSQRPVA
                     GVGNIYADEALWLARIHPASRRVGRPRAHALHAAIREVLAAAVAREGTTFRDYRMMNG
                     ESGRNADFLVAYGQAGRACPRCGTALARIVLGGRGATFCPTCQRR"
     gene            1877..2812
                     /locus_tag="VEP73_06260"
     CDS             1877..2812
                     /locus_tag="VEP73_06260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYT26066.1"
                     /translation="MRTPVVLVAEDCRVVRTFLTRHLEAHGYRVVEAADGGQAVARCR
                     RERPDVVLLDVEMPFMDGHEVLASMKADPALADIPIVFVTARVTTEDVVAGLSLGAHD
                     YLRKPFEPSELLARVHAALRTKQLRDELRLRNAELDVMSRTDSLTRLHNRRHLEERLG
                     ELVSLLRRHGGELSLLLIDVDHFKRVNDEHGHATGDAVLRVLAARLKDSIRAEDVPGR
                     WGGEEFVVLLPSTGPEGAHAAGERVRLAVATSPFALAGGGLRVTVSVGVASGIDGGWE
                     GLVQRADAALYAAKEGGRNRVVVAGEPSSAGTPAR"
     gene            complement(2834..3739)
                     /locus_tag="VEP73_06265"
     CDS             complement(2834..3739)
                     /locus_tag="VEP73_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT26067.1"
                     /translation="MSWVLQRPPPPQTATGSAPRGAGRVPAPWGEPFRVLVIEDSPSD
                     ARLVEEMLETAWPPGLGTPLEIVHVERLADAYPHLSAGVACVLLDLWLPDAAGLESML
                     QVRVADPAVPVIVLSGLDDEGLAVEAVHRGAQDYLMKGQADGPLMSRAIRYAVERKRI
                     EAELEAREELNRAVLDSMPAAVAVLDAQAGIVSVNQAWAVRAAQHGDDVLWSGVGMGY
                     AEACARAGGPGPADADRAMAGIRAVLAGAERNLSMAYTVATPDGERRCVLHVWPLALG
                     QGGVVVSHAGLDGLEPPGDPERPAS"
     gene            3910..4653
                     /locus_tag="VEP73_06270"
     CDS             3910..4653
                     /locus_tag="VEP73_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26068.1"
                     /translation="MRERSNAELERFAVVAAHELKEPLRAVAGHARLLGQRYQGRLGA
                     DADELIGFVTAGVARMEALVDALLAYARAGPDRTAHAPVPAEEAADRAVANLRHAIDE
                     SGATVERGPLPVVRGDAAQLVQLFQNLVGNAVKFRAARPPLVRISAERSSAWWRFAVA
                     DNGIGVEPRLAQRAFDMFQRLHGRGEYPGTGIGLAVCARIVAGHGGAIWLEPAPGSGT
                     TCFFTLPACRPVGPAARQEQGRRRPRRKG"
     gene            4708..5646
                     /locus_tag="VEP73_06275"
     CDS             4708..5646
                     /locus_tag="VEP73_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HYT26069.1"
                     /translation="MDTPATRVLLVMDDTGSASRVRAALAAVRDRRLDVVSAARLEDT
                     LGLLAAAELDVALVDCSLPDGPGRATFRAVQEQVPGLPVIVLHAPGADAAGQQAVTEG
                     AADHLSTGSLDPELVSRAIRYAVERQQLLGDLRRLALVDGATGIANRRGFVAMSEGLV
                     RLAERTGLTVTVLYADVDGLEEINEAFGHAEGDRALRAVAKLLRATFRASDVVARVGG
                     DEFCVLLLHDGVGESPAVRRLDEAVAAARTPGGRYALSLSLGVAHCTPGRPASVEELV
                     ERAAGAMRDGRARRSRAAGPAGPRLVAPVATWGERW"
     gene            5783..5977
                     /locus_tag="VEP73_06280"
     CDS             5783..5977
                     /locus_tag="VEP73_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26070.1"
                     /translation="MIGKVGRVTGTVAPGLIGEVMIPVRGGTEAFNAYAADARDTIQT
                     GTRIVVVEYFPPRTVVVSPL"
     gene            5998..7503
                     /locus_tag="VEP73_06285"
     CDS             5998..7503
                     /locus_tag="VEP73_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYT26071.1"
                     /translation="MNSALYLAGGVVAVLIVLFILFKAMWRVAEPNEALVISGLREHA
                     EPEGVGESLGFKIVTGKGTFVIPGVQQVRRLSLDLRETQLAIECVTHQGIPLGIQGVV
                     IFKVGDDYASIANAARRFLDQQQQMDARVHNVFAGHLRAIVGNMTVEEMIRDREKLTS
                     LTRASSGTEMEKLGLIVDSLQIQEIHDPTGYIKNLALPHAAAVSREARIAQAGADREA
                     TQAEQEADALKAAARRESQIKQAGYQAEVDRAAAEARQAGPLSEATARQNVVVQETKV
                     AELEGQREEQRLQASVRKPADAKAYQQVTLAKAERDARVAGAEARRQEVELDAAANAQ
                     RVKLEAEADAQRVRAEAAARSESTRLVGEAEASATQAKGRAEGEAVRARGMAEAESIK
                     ARADALAENQDAVIGQQLAEQWPAIVEAAAKPFAAVDQMIVLNGAQGLSEALAAALSQ
                     GAAGLQLARNLLAGTGNGSKDGRAAGGDGTPPAAATIEPQPGRRAGEGPRR"
BASE COUNT          977 a         2822 c         2878 g          886 t
ORIGIN      
        1 tagaccatcg actcgatgcc cccgtagcca tgcgggggga tcgagtacca gggtggggcg
       61 acgagcgcga tccggagcgg cgagccatcc tcgtgcgtgg gcactggcac ctcctgagcc
      121 agacgggatg ctcgaatcta acaaacggat cacgctcgta ccgcaatcca gcagctcccg
      181 gcgaacgggc ggagcgcgca gccggagccg gagccgcccc ggtttgccgt tcctggcggc
      241 ggtcaggctc cgtggcgaac ctctgccggc acggcgccgg gcagccgtgc cgcctgctca
      301 ggattaagtc cgcgccggcg gggtacggcg gcccggtcca gctacgcggc ggtcaagcgc
      361 gcctgccagg gcaggtagga ggagccgcgg cgaggaaggg agcagccgat gggggcgccc
      421 agcgggcccg ccgtcgccgt tggcctcgtc gccagggacc cgcgcgggcg cctgctcctg
      481 gtgcggcggg gtcacccgcc cgcgcggggc gcctggtccc tgcccggggg gcgggtcgag
      541 gcaggcgaga ccatggccga ggcggcagcc agggagctgc gcgaggagac cgggctcacc
      601 gcgcgggtcg gcgccgtcgc cggggtggtc gagcggatcg cagagggcta ccactacgtc
      661 atcgtcgacc tctgggccga gctggacggc gccgagccgg tcgccgggga cgacgccacg
      721 gcggcccggc tcgtcgagcc gtccgagctg ccagcgctcg acctgagccc agggctcggc
      781 gagttcctgg ccgggatcgg ctgctggccg gacggcgccc ccccgccaga gccctaccag
      841 ccggagccgt cgtagcggcg ggcgccggag ccgcggcagc ggccgggagg cgaccggcca
      901 ggccggcacc gcggtgcgcg ccccgctagg ctggcggcat gccggagctt cccgaggtcg
      961 agtcggtccg ccggcagctc gagccggagc tgcgcggccg gcgcgtgctg cgtgtccggg
     1021 ccgacgccca gcgccgcttc cacgccgtcg agcggctcgc cggccgccgg gtcgaggcgg
     1081 tgggacggcg gggcaagttc atcctctgcc ccctggacgc cgggctcgag ctggtcctgc
     1141 acctcgggat gaccgggtcg ctgcacttcg gcccggccga cgcgtacgcg cgcgcactgc
     1201 tcgccctcga cgacggccgc gagctggtct tccgcgacgt gcgacggttc ggctgcgtcg
     1261 ccgtcgtcga ggcgggcgcc tacgggccgc tgccgacgct cgcccggctc ggccccgagc
     1321 cgctgtcgga cgacttcgac ccggaccggt tcgcccggga cctggccgtg accagggcgc
     1381 cggtcaagcc gtacctgctc tcgcagcgac cggtcgcggg cgtcggcaac atctacgccg
     1441 acgaggcgct ctggctcgcc cgcatccacc ccgcctcgcg ccgggtcggg cggccgcgcg
     1501 cccacgcact gcacgcagcg atccgcgagg tcctcgcggc agccgtcgcc agggagggca
     1561 cgaccttccg cgactaccgg atgatgaacg gcgagagcgg gcgcaacgcc gacttcctgg
     1621 tcgcctacgg ccaggcgggc cgcgcctgcc cccgctgcgg gacggcgctc gcgaggatcg
     1681 tcctcggcgg ccgcggcgcg accttctgcc ccacctgcca gcgccgctga ccctggccgc
     1741 cgggcggcac gcccggcgct caaccggggc ggccacgccc cggcgctcaa cccgggcggc
     1801 cacgggccga tggagagcgg gacggcccgg tgcgcgccgg gcgcaccggc agcccagcgc
     1861 ggaggggagg gcgccgatga ggaccccggt cgtgctggtg gccgaggact gccgggtggt
     1921 ccgcacgttc ctcaccaggc acctcgaggc gcacggctac cgggtcgtgg aggccgccga
     1981 cggcgggcag gcggtcgcgc ggtgccggcg cgagcgaccc gacgtcgtgc tgctcgacgt
     2041 ggagatgccg ttcatggacg gccatgaggt gctcgccagc atgaaggcgg acccggcgct
     2101 ggcggacatc ccgatcgtgt tcgtcaccgc ccgggtgacc accgaggacg tcgtcgccgg
     2161 gctgtcgctg ggcgcgcacg actaccttcg caagccgttc gagccgagcg agctgctcgc
     2221 cagggtgcat gccgcgctgc ggaccaagca gctgcgggac gagctgcgcc tccgcaacgc
     2281 ggagctcgac gtcatgagcc ggaccgactc cctgacccgg ctccacaacc gccgccacct
     2341 ggaggagcgg ctgggcgagc tggtcagcct cttgcggcgc cacggcggcg agctgtcgct
     2401 cctcctcatc gacgtcgacc acttcaagcg cgtcaacgac gagcacgggc acgcgaccgg
     2461 cgacgcggtg ctgcgcgtcc tcgccgcccg gctcaaggac tcgatccggg ccgaggacgt
     2521 gccgggccgc tgggggggcg aggagttcgt cgtgctcctg ccctcgaccg ggccggaggg
     2581 ggcgcacgcc gccggggagc gcgtccgcct ggcggtcgcg acctcgccgt tcgcgctggc
     2641 cggcggcggg ctgcgggtca ccgtgagcgt cggcgtcgcc tcggggatcg acggcggctg
     2701 ggaggggctg gtccagcggg ccgacgccgc gctctacgcc gccaaggagg gcggccgcaa
     2761 ccgggtcgtc gtcgcgggcg agccaagctc ggccggtacg ccggcacggt aggcagcacg
     2821 ggtcgagagc cggtcagctc gcgggccgct ccgggtcccc gggcggctcg agcccgtcca
     2881 gcccggcatg gctcaccacc acgccgccct ggcccagggc gagcggccag acgtgcagga
     2941 cgcagcgacg ttcgccgtcg ggcgtggcga ccgtgtacgc catcgacagg ttccgctcgg
     3001 cgccggcgag caccgcacgg atgcccgcca tggcccggtc cgcgtcggcc ggccccgggc
     3061 ccccagcccg cgcgcacgcc tccgcgtagc ccatgccgac gccggaccac agcacgtcgt
     3121 cgccgtgctg ggcggcgcgg acggcccagg cctggttgac gctcacgatc ccggcctgcg
     3181 cgtcgagcac ggcgacggcg gcgggcatgg agtcgagcac cgcccggttg agctcctcgc
     3241 gcgcctcgag ctcggcctcg atccgcttgc gctccaccgc gtagcggatc gcccggctca
     3301 tcagcggccc gtcggcctgc cccttcatca ggtagtcctg cgcgccccgg tgcaccgcct
     3361 cgacggccag cccctcgtcg tcgagcccgg acagcacgat gaccggcacc gccgggtcgg
     3421 cgaccctgac ctggagcatc gactcgagcc cggccgcgtc cggcagccac aggtcgagca
     3481 gcacgcaggc gacgccggcg gagaggtggg gataggcgtc ggcgagccgc tcgacgtgga
     3541 cgatctcgag cggggtgccg agcccgggcg gccacgcggt ctccagcatc tcctcgacca
     3601 ggcgggcgtc ggacgggctg tcctcgatca cgagcacccg gaagggctcc ccccatggcg
     3661 ccgggacccg gccggcgccg cggggcgcgc tgccggtggc cgtctgcgga ggcggcggcc
     3721 gctgcagcac ccaggacatc gagcctcctc ccccaccggt cggggcggac ggtgggtccc
     3781 gactagtgtc ggcagcttcg cgaacgtcgt tgagccgccg ccccggaggg acggcgccgg
     3841 ttcgggtccg gttcagggcc gggccgccgt gccgataccc aattggacag gaccgttcgg
     3901 gggtggggca tgcgcgagcg ttcgaacgcc gagctcgagc ggttcgcggt cgtggccgcc
     3961 cacgagctga aggagccgct gcgggcggtg gccggtcacg cgaggctgct cggccagcgg
     4021 taccagggac ggctgggcgc cgatgccgac gagctgatcg gcttcgtcac cgccggggtg
     4081 gcgcgcatgg aggcgctcgt cgacgccctg ctcgcctacg ccagggccgg cccggaccgg
     4141 accgcccatg ccccggtgcc tgccgaggag gccgccgacc gggcggtcgc gaacctgcgc
     4201 cacgccatcg acgagagcgg cgcgacggtc gagcgggggc cgctcccggt cgtccgcggc
     4261 gacgccgccc agctcgtcca gctcttccag aacctcgtcg gcaacgccgt gaagttccgc
     4321 gcggcgcggc cgccgctcgt gcgcatctcg gccgagcgct cgagcgcgtg gtggcgcttt
     4381 gccgtggccg acaacgggat cggcgtcgag ccgcggctcg cccagcgggc gttcgacatg
     4441 ttccagcgcc tgcacggccg cggggagtac cccggcaccg ggatcgggct cgccgtctgc
     4501 gccaggatcg tcgcgggcca cggcggcgcc atctggctcg agcccgcacc gggctcgggc
     4561 accacctgct tcttcaccct tcccgcctgt cgcccggttg gtccggcagc ccgccaggag
     4621 cagggtcgtc gtcgcccccg tcgaaaggga tgacccgggc cccgaggcgc ccgtcacccc
     4681 gtcaccgcac ccgtggagga gcagctcatg gacacgccgg ccacccgggt cctgctggtc
     4741 atggacgaca ccggtagcgc cagccgggta cgggccgccc tcgcggccgt ccgggaccgc
     4801 cgcctcgacg tcgtctcggc cgcgcggctc gaggacacgc tcggcctcct ggccgcggcc
     4861 gagctggacg tcgccctggt cgactgctcg ctgcccgacg gtcccggacg ggccacgttc
     4921 cgggcggtcc aggagcaggt tcccgggctg cccgtcatcg tgctgcacgc gccgggcgcc
     4981 gacgcggccg gccagcaggc ggtgaccgaa ggggccgccg accacctgtc gaccggatcc
     5041 ctcgaccctg agctcgtgtc ccgcgcgatc cggtacgcgg tggagcgcca gcagctcctc
     5101 ggcgacctgc gccggctcgc gctggtcgac ggcgccaccg ggatcgccaa ccgccggggg
     5161 ttcgtcgcca tgagcgaggg cctggtccgg cttgccgagc gcacagggct caccgtcacc
     5221 gtcctgtacg ccgacgtcga cggcctcgag gagatcaacg aggcgttcgg ccacgccgag
     5281 ggcgaccgcg ccctgcgcgc cgtcgccaag ctgctgcggg ccacgttccg agcgtccgac
     5341 gtggtcgcgc gtgtcggcgg cgacgagttc tgcgtgctcc tgctccacga cggcgtcggc
     5401 gagagcccgg cggtccgccg gctcgacgag gcggtcgccg cggcgaggac cccgggcggc
     5461 cgctacgcgc tgtcgctcag cctcggcgtc gcgcactgca ccccgggccg gccggcctcg
     5521 gtcgaggagc tcgtcgagcg ggccgccggc gccatgcgcg acggccgggc caggcgctcg
     5581 cgggcggcgg gcccggccgg tcctcggctg gtggctccgg tggcgacctg gggcgagcgg
     5641 tggtagccgc gccgcgatat cgaattcgcg cagattcgcc tggcctctgg acacccggct
     5701 ccggaagtgg tcaaacagta gcgaaggcgc gaggcagctc ccaggcgagc gaggaatcag
     5761 ggatccggtg gccgaccaca cgatgatcgg gaaagtggga cgggtcacgg gcacggtcgc
     5821 accagggctg atcggcgagg tcatgatccc ggtccggggg ggcaccgagg cgttcaacgc
     5881 ctacgcggcc gacgcccgcg acacgatcca gaccgggacc cggatcgtcg tcgtcgagta
     5941 cttcccgccg aggaccgtcg tggtgagccc gctgtagggc tcgttggagg catcctcatg
     6001 aactccgctc tgtacctggc cggtggggtc gtcgccgtcc tcatcgtgct gttcatcctg
     6061 ttcaaggcca tgtggcgggt cgccgagccg aacgaggcgc tcgtcatctc cggcctgcgc
     6121 gagcacgccg agcccgaggg cgtcggcgag agcctcggct tcaagatcgt gaccgggaag
     6181 ggcacgttcg tgatccccgg cgtccagcag gtgcggcggc tctcgctgga cctgcgcgag
     6241 acccagctcg ccatcgagtg cgtcacccac caggggatcc ccctcgggat ccagggcgtc
     6301 gtcatcttca aggtgggcga cgactacgcc tccatcgcca acgccgcccg ccgcttcctg
     6361 gaccagcagc agcagatgga cgcccgggtc cacaacgtgt tcgccggcca tctgcgcgcg
     6421 atcgtcggca acatgaccgt cgaggagatg atccgcgacc gggagaagct gacctcgctg
     6481 acccgcgcct cctcgggcac cgagatggag aagctcggac tgatcgtgga ttccctgcag
     6541 attcaggaga tccacgaccc cacgggctac atcaagaacc tggccctgcc gcatgccgcc
     6601 gcggtctccc gggaggcccg catcgcccag gcgggcgccg accgcgaggc gacccaggcc
     6661 gagcaggagg cggacgcgct caaggcggcc gcccggcgcg agagccagat caagcaggcc
     6721 ggctaccagg ccgaggtcga ccgggccgcc gccgaggcca ggcaggcggg accgctgtcg
     6781 gaggcgaccg ccaggcagaa cgtcgtcgtc caggagacga aggtcgccga gctcgagggg
     6841 cagcgcgagg agcagcgcct gcaggcttcg gtgcgcaagc cggccgacgc caaggcgtac
     6901 cagcaggtga ccctggccaa ggccgagcgc gacgcccgcg tcgccggcgc cgaggcccgc
     6961 cgccaggagg tcgagctgga cgccgcggcg aacgcccagc gggtgaagct cgaggcggag
     7021 gccgacgcgc agcgggtgcg ggccgaggcg gccgcccgct ccgagtcgac ccggctcgtc
     7081 ggcgaggccg aggcgtccgc cacgcaggcc aagggccgcg ccgagggcga ggcggtccgc
     7141 gcccgcggca tggcggaggc cgagtcgatc aaggccaggg ccgacgcgct cgccgagaac
     7201 caggacgcgg tcatcgggca gcagctcgcc gagcagtggc cggccatcgt ggaggcggcc
     7261 gcgaagccgt tcgccgccgt ggaccagatg atcgtgctga acggcgccca ggggctgtcc
     7321 gaggcgctcg ccgcggcgct cagccagggg gcggccgggc tccagctcgc ccggaacctg
     7381 ctcgccggga ccggcaacgg cagcaaggac ggcagggcgg cgggcggcga cgggacgccc
     7441 cccgccgcgg cgaccatcga gccccagccc ggccggcgcg ccggggaggg cccgcgccgc
     7501 tgagcagctt ggcggcgccg tcgacgaagc ggaggtcacg cgcgtgcacg tggcagtcac
     7561 cgg
//
LOCUS       DASPYY010000405         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2457571, whole genome shotgun sequence.
ACCESSION   DASPYY010000405 DASPYY010000000
VERSION     DASPYY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457571"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..502
                     /gene="rimI"
                     /locus_tag="VEP73_06290"
     CDS             83..502
                     /gene="rimI"
                     /locus_tag="VEP73_06290"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYT26072.1"
                     /translation="MPAIISLERELFPEDAWTPEMFAAEFAQPPSRRLYLVAEDGNVL
                     VGYAGMMFTGGSQADVVTLAVTPARWGEGTGTALLTALVDEADKRGYEEVLLEVREDN
                     PRARRLYLRHGFTEVGIRRGYYQPSGVDAVVMRKGLR"
     gene            499..1533
                     /gene="tsaD"
                     /locus_tag="VEP73_06295"
     CDS             499..1533
                     /gene="tsaD"
                     /locus_tag="VEP73_06295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYT26073.1"
                     /translation="MMVSPLVLGIETSCDETGIGLVRGHDLLADAVASSVDEHARFGG
                     VVPEVASRAHLEAMIPTFDRALATAGVRPGQIDAVAVTAGPGLAGALLVGVAAAKAFS
                     FALGKPLYGVNHLAAHIAVDQLEHGTLPSPAVALLVSGGHSSLLLVPDVAEEVIPLGA
                     TIDDAAGEAYDKVARVLGMPFPGGPPIDKAARDGDPAAISFPRGKYHDGTLDFSFAGL
                     KTAVARWVEARQAAGQPVHAPDVAASFQEAVADVLTRKAVQACQRNDVTDLIIGGGVA
                     ANSRLRSLAAERCDAAGIKLRVPRPGLCTDNGAMVAALGAEIVSRGLPPSPLDLPADS
                     SLPVTDVVVR"
BASE COUNT          209 a          599 c          639 g          222 t
ORIGIN      
        1 ccacctcccc agagccctcg tcatgagcgc ctgcacgaac ggccagggca cgatacggct
       61 gcgggggatg aagcgggccg acatgcccgc gatcatctcg ctggagcggg agctcttccc
      121 cgaggacgcg tggacgccgg agatgttcgc ggcggagttc gcgcagccgc cctcccgccg
      181 gctctacctg gtggccgagg acggaaacgt gctggtcggg tacgcgggga tgatgttcac
      241 cggagggtcg caggccgatg tggtgacgct ggcggtgact ccggcccggt ggggcgaggg
      301 gaccggtacg gcgctgctga cggcgctggt cgacgaggcg gacaaacgcg gttacgagga
      361 ggtcttgctc gaggtgcggg aggacaaccc cagggccagg cggctgtacc tgcgccacgg
      421 cttcaccgag gttgggatca ggcgcggcta ttaccagccg tccggcgtcg acgccgtggt
      481 catgcggaag gggctgcggt gatggtctcc ccgctggtac tgggcatcga gacgtcctgc
      541 gacgagacgg gcatcgggct ggtccgtggt cacgacctgc tcgccgacgc cgtcgcgtcg
      601 agcgtggacg agcacgcgcg cttcggcggg gtggtgcccg aggtggcgag ccgggcgcac
      661 ctggaggcga tgatcccgac ctttgaccgg gccctggcga ccgcgggcgt ccggcccggc
      721 cagatcgacg ccgtggccgt caccgcgggg ccgggcctgg ccggggcgct gctggtcggg
      781 gtggccgcgg ccaaggcgtt ctccttcgcg ctgggcaagc cgctgtacgg ggtcaaccac
      841 ctggccgcgc acatcgccgt agaccaactg gagcacggca cgctgccgtc gcccgcggtg
      901 gccctgctgg tgtccggcgg gcactcctcg ctgctgctcg tgccggacgt ggccgaggag
      961 gtcatcccgc tcggggcgac catcgacgac gcggccggcg aggcctacga caaggtggcg
     1021 cgggtgctcg gcatgccgtt tcccggcggc ccgccgatcg acaaggcggc ccgcgacggc
     1081 gaccccgccg cgatcagctt cccccggggc aagtaccacg acggcacgct ggacttctcc
     1141 ttcgcgggcc tgaagaccgc ggtggcccgc tgggtggagg cgcggcaggc ggccggccag
     1201 ccggtacacg ccccggacgt ggccgcgtcc ttccaggagg cggtggccga cgtgctcacc
     1261 cgcaaggccg tgcaggcctg ccagcggaac gacgtcaccg acctgatcat cggcgggggc
     1321 gtggcggcca attcccggct gcgctcgctg gccgccgagc gctgcgacgc ggccggcatc
     1381 aagctgcgcg tgccgcggcc gggcctgtgc accgacaacg gcgccatggt cgccgccctg
     1441 ggcgcggaga tcgtgtcacg cggcctgccg ccctccccgc tcgacctgcc cgcggactcc
     1501 tccctccccg tcaccgacgt cgtcgtccgc tagccggcca ccgtaccccg ggcgcacctg
     1561 gcgtcgcggc ccgggcgcgc ccgggcgccc ggcctagtcc tccttgctgc tcccgccgcg
     1621 gcggaacagg tgggaagcgc cgggcgagca gggcgagcgc ggccacggc
//
LOCUS       DASPYY010000406         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_631787, whole genome shotgun sequence.
ACCESSION   DASPYY010000406 DASPYY010000000
VERSION     DASPYY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631787"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..560)
                     /locus_tag="VEP73_06300"
     CDS             complement(39..560)
                     /locus_tag="VEP73_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HYT26074.1"
                     /translation="MDTDPVDGRIGEGWAGEDPNGSHVNVVAARRGSATGAAVAAALA
                     SPRPGHLPFIACLERGAMVRPTTVVVNKVPLEGEAQSRIVWGAAQLGIAQGVLDEVAE
                     GTVDPGDAAELLLLVAVWIDPAAHDETAVRKASRTATSRALADALRPPSAEEVGALAA
                     RREAASTPFYGGD"
     gene            complement(610..1716)
                     /locus_tag="VEP73_06305"
     CDS             complement(610..1716)
                     /locus_tag="VEP73_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HYT26075.1"
                     /translation="MTTVLAVVLPGTGARLEVTQVDLAPPGPGEVLVRLHASGVCHSD
                     LNAIDGTAPTRCPAVLGHEGAGVVEQPGPGVSLRPGQPVALSWIPWCGRCEECLRGLA
                     NLCRTAWGAMGHGGLLDGTPRLSRLGEPVYHYSLLSTFAERAVVAERSCVPIDADVPF
                     GVAALVGCAVATGVGAVWNTAGVRPGERVAVLGCGGVGLSAVLGAVAVGATPVVAVDV
                     SSEKLEVALALGATDAVPWAGDPEATAEAVRAASGGGVDYAVEATGRPEVAEAAFGST
                     RARGAAVLVGIPRADAELRLPALSVPRMERRVLGSAYGSTQPARDFPRILALYRSGRL
                     PLDRLISHRLPLREVEAAFALLRSGGARRVLLEP"
     gene            complement(1713..>2256)
                     /locus_tag="VEP73_06310"
     CDS             complement(1713..>2256)
                     /locus_tag="VEP73_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26076.1"
                     /translation="GDPRVVVCDEPTSALDVSVQAAILNLLVDLQRDEGVTYLFISHD
                     LGVVRFVADRIAVLYLGRLMEVGPADVVFRGPHHPYTEALLSAAPILFTGQDQEQGRG
                     ATRERIRLSGEIPSAADPPSGCVFHTRCPRKLGAICEETVPPLIEVEPGHFMACHIPV
                     EELRRLQQGTTGSLATEPGR"
BASE COUNT          284 a          881 c          852 g          239 t
ORIGIN      
        1 tcgacggccg cgatgcgcac gctcggtcac ccgccgccct agtcgccgcc gtagaagggc
       61 gtgctcgcgg cctcgcggcg cgccgcaagc gcgccgacct cctcggccga gggaggccgc
      121 agcgcgtcgg cgagggcgcg ggaggtggcg gtgcggctgg ccttgcggac cgcggtctcg
      181 tcgtgtgcgg cggggtcgat ccagaccgcg acgagcagca gcagctcggc cgcgtcgcca
      241 gggtcgaccg tcccctcggc gacctcgtcg agcacgccct gggcgatccc gagctgggcc
      301 gcgccccaga cgatccggga ctgggcctcg ccctcgagcg gcaccttgtt caccacgacg
      361 gtggtgggac gcaccatcgc gccacgctcc aggcaggcga tgaacggcag gtgcccgggg
      421 cgcggggagg cgagcgcggc cgccaccgcc gcgcccgtgg ccgagccgcg ccgggccgcg
      481 acgacgttga cgtggctgcc gttcgggtcc tcgccggccc agccctcgcc gatgcgcccg
      541 tccacggggt cggtgtccat cacgcctcct tcgtccgggt ggcggcgcgt cgtcggcccg
      601 ccgtcgccgt cagggctcga gcaggacccg gcgcgccccg cccgagcgta gcagcgcgaa
      661 cgccgcctcg acctcccgga gcggcaggcg gtgggagatc aggcggtcga gcgggaggcg
      721 ccccgagcgg tacagcgcga ggatcctcgg gaagtcccgc gccggctgcg tcgagccgta
      781 cgcgctgccg agcacccggc gctccatccg ggggaccgag agcgccggca ggcgcagttc
      841 ggcgtcggcg cgcgggatcc ccaccaggac cgctgcgccc cgcgcgcgcg tcgacccgaa
      901 cgccgcctcc gcgacctctg gccggccggt ggcctcgacc gcatagtcca ccccgccgcc
      961 gctcgccgcg cgcaccgcct cggcggtcgc ctccgggtcg ccggcccagg gcacggcgtc
     1021 ggtcgcgccc agcgcaaggg ccacctcgag cttctcggac gagacgtcga cggccaccac
     1081 cggcgtggcg ccgaccgcga ccgcgccgag gacggcggac agccccaccc cgccgcagcc
     1141 gagcacggcg acgcgctcgc cggggcgcac gcccgccgtg ttccagaccg ccccgacgcc
     1201 ggtcgcgacc gcgcagccga cgagcgccgc caccccgaac ggcacgtcgg cgtcgatcgg
     1261 cacgcaggag cgctcggcga ccaccgcgcg ctcggcgaag gtggacagca gcgagtagtg
     1321 gtagacgggc tcgccgaggc gcgacagccg cggcgtgccg tcgagcaggc cgccgtggcc
     1381 catcgccccc caggccgtgc ggcacaggtt ggcgaggccc cgcaggcact cctcgcaccg
     1441 cccgcaccac gggatccagg agagcgccac cggctggccg ggtcgcagcg acacgcccgg
     1501 ccccggctgc tccaccaccc cggcgccctc gtgccccagc accgcggggc agcgcgtcgg
     1561 cgcggtgccg tcgatggcgt tgaggtcgga gtggcacacg ccgctggcgt ggagccgcac
     1621 gagcacctcg cccggtccgg gaggcgccag gtcgacctgg gtcacctcga gcctggcgcc
     1681 ggtgccgggc aggacgacgg cgaggaccgt cgtcacctcc cgggctcggt ggccaggctc
     1741 ccggtcgtgc cctgctggag gcgccgcagc tcctcgacgg ggatgtggca ggccatgaag
     1801 tggccgggct cgacctcgat cagcgggggc acggtctcct cgcagatcgc gccgagcttg
     1861 cgggggcagc gcgtgtggaa cacgcagccc gagggcgggt cggcggcgct cgggatctcg
     1921 ccggagagcc ggatgcgctc gcgggtcgcg ccgcggccct gctcctggtc ctggccggtg
     1981 aacaggatcg gcgccgccga cagcagcgcc tcggtgtagg ggtggtgcgg tccccggaac
     2041 acgacgtcgg ccggccctac ctccatgagt cgcccgaggt acagcaccgc gatccggtcc
     2101 gcgacgaagc gcaccacgcc caggtcgtgg gagatgaaca ggtaggtgac gccctcgtcg
     2161 cgctgcaggt ccaccagcag gttgaggatc gcggcctgca ccgagacgtc gagcgccgag
     2221 gtgggctcgt cgcacaccac cacgcgcgga tccccg
//
LOCUS       DASPYY010000407         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1754876, whole genome shotgun sequence.
ACCESSION   DASPYY010000407 DASPYY010000000
VERSION     DASPYY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754876"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VEP73_06315"
     CDS             complement(<1..237)
                     /locus_tag="VEP73_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006431182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYT26077.1"
                     /translation="MEDRHDDHPPETGAHASIGRRLFADRVEAGDRLAEALAERVIEP
                     VVVLGVPRGGVIVADRVARRLGAALDVVVPRKLGA"
     gene            473..706
                     /locus_tag="VEP73_06320"
     CDS             473..706
                     /locus_tag="VEP73_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYT26078.1"
                     /translation="MVTAYILIQTEVGKAAQVAKEIAEIKGVQQAEDVTGPYDVIVRA
                     EARNVDDLGKLVVARVQAVDGITRTLTCPVVHL"
     gene            complement(762..1895)
                     /gene="serC"
                     /locus_tag="VEP73_06325"
     CDS             complement(762..1895)
                     /gene="serC"
                     /locus_tag="VEP73_06325"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HYT26079.1"
                     /translation="MTREPTTLSLPDELRPRDGRFGSGPSKVRQEAVAALAEAAPAYL
                     GTSHRQAGVRSMVARLRAGLAEFFHLPAGYEVVLGNGGASVFWDVAAFSLIERRSQHL
                     VFGEFSAKFAAAAGAPHLAAPEIIESPYGTHPEPRADPDVDLYAWPHNETSTGVAMPV
                     RRPEGAAGLVCVDATSGAGGLPVDPAEFDAYYFAPQKSFGSDGGLWVAILSPAALDRA
                     AAIGASGRRVPLSLSLPVAIESSRADQTWNTPALATIFLFVHQLEWMNRQGGLDWAAK
                     RCDESAAVVYGWAEASPYATPFVADPAARSHTVATIDFDERVDAAAVARALRANGIVD
                     TEPYRRLGRNQLRIAMFPAVEPGDLETLTRAVDHVVRALGQPG"
     gene            1943..>3212
                     /locus_tag="VEP73_06330"
     CDS             1943..>3212
                     /locus_tag="VEP73_06330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYT26080.1"
                     /translation="MTRISRRVGAIAESATLAIDAKAKGMKAAGEDVIGFGAGEPDFP
                     TPDHIVEAAAAACRVPRFHRYTPTAGLPELREAIAAKTARDSGYAVPPGQVLVTNGGK
                     QAIFEAFATLLDPGDEVLLPAPYWVTYPEAITLAGGVPVVVPTEAATGFRATVEQLEA
                     ARTPRTKVLLFVSPSNPTGAVYPRAEMEAVGRWAADRGLWVVTDEIYEHLVYGDSRFH
                     SLPVLVPELADRCVVVNGVAKTYAMTGWRVGWMLAPPDVVAAATNLQSHATSNVANVA
                     QAAALAAVSGDLSDAARMRAAFDRRRRRIHELLTAIPGVACMEPQGAFYAFPSFARML
                     GRELRGRTPASTLELAEVILDEAKVAIVPGEAFGAPGYARLSYALGDDDLVEGIRRVE
                     LLDGGQVPGGLVGRHGHRHAARQRDRLRIGG"
BASE COUNT          422 a         1171 c         1202 g          417 t
ORIGIN      
        1 agcgccgagc ttgcgcggca cgacgacgtc gagggccgcg cccaggcggc gggccacccg
       61 gtccgcgacg atcaccccgc cgcgcgggac ccccaggacc acgaccggct cgatgacgcg
      121 ctcggccaag gcctcggcga gccggtcgcc agcctcgacc cggtcggcga acagccggcg
      181 ccctatcgag gcgtgcgctc cagtctcggg cgggtggtca tcgtgacggt cctccatggt
      241 cggccggcac gtccagccgg cccgtcgcgg ctggaccgtt catggtagcc cgggatcgac
      301 ccccgccgga agcgtcggct tccgtcccgg cccgccgccc attccttggc agggttgggg
      361 ggctcgtata gcattcgcag atctctctac agacccctga aggggtgccg tgtccccttc
      421 tcgggaccgc gccagggcgg cggctcgggc acagccaagg aggtcggtgc ttgtggtgac
      481 ggcgtacatc ctcatccaga ccgaggtggg caaggcggcg caggtcgcca aggagatcgc
      541 cgagatcaag ggcgtccagc aggccgagga cgtcacggga ccgtacgacg tcatcgtccg
      601 ggccgaggcg cgcaacgtcg acgacctcgg caagctcgtc gtcgcccgcg tgcaggcggt
      661 ggacggcatc acccgcaccc tcacctgccc ggtggtgcac ctctagggag cccacaggac
      721 cgtggacgtg tcccgccggc ggccggggcc gggccgccgg ttcagcctgg ctgcccgagc
      781 gcgcgtacga cgtggtcgac cgcccgggtg agcgtctcca ggtcgcccgg ctcgaccgcg
      841 gggaacatcg cgatgcgcag ctggttgcgg ccgagcctgc ggtacggctc ggtgtcgacg
      901 atcccgttcg cgcgcagggc cctggcgacc gccgcggcgt cgacgcgctc gtcgaagtcg
      961 atcgtggcca ccgtgtggct ccgcgccgcc gggtcggcga cgaagggggt ggcgtacggg
     1021 ctcgcctccg cccagccgta gacgaccgcg gccgactcgt cgcagcgctt cgcggcccag
     1081 tcgagaccgc cctgccggtt catccattcg agctggtgga cgaacaggaa gatggtcgcg
     1141 agcgcggggg tgttccaggt ctggtcggcc cgggagctct cgatcgcgac cggcaggctg
     1201 agcgacagtg gcacgcggcg gccggaggcg ccgatcgccg cggcccggtc gagggcggcg
     1261 ggcgacagga tcgccaccca cagcccgccg tcggagccga agctcttctg cggcgcgaag
     1321 tagtaggcgt cgaactcggc cgggtcgacc ggcaggccgc cggcgcccga ggtggcgtcg
     1381 acgcagacga gcccggccgc tccctcgggg cggcggaccg gcatggcgac gccggtcgac
     1441 gtctcgttgt ggggccacgc gtagaggtcc acgtccgggt cggcgcgagg ctcggggtgg
     1501 gtgccgtacg ggctctcgat gatctcggga gcggcgaggt ggggggcgcc ggccgccgcg
     1561 gcgaacttgg ccgagaactc gccgaacacg aggtgctggc tgcgccgctc gatcagcgag
     1621 aacgcggcca cgtcccagaa gacgctcgcg ccgccgttgc cgaggacgac ctcgtacccg
     1681 gcgggcaggt ggaagaactc ggccagcccg gcgcggagcc gagccaccat cgagcgcacc
     1741 ccggcctgcc ggtggctcgt gccgaggtag gcgggggcgg cctcggccag cgcggcgacc
     1801 gcctcctgcc tgaccttgga cgggcccgag ccgaagcgcc cgtcgcgcgg gcgcagttcg
     1861 tccggcaggg aaagggtggt gggttctcgg gtcatgtgtg ccactgtatc gtcgcaggct
     1921 tgctcaagga ggcatgcatt ccatgacgcg catctcccga cgggtcggcg ccatcgccga
     1981 gtcggccacc ctcgcgatcg acgccaaggc caaggggatg aaggcggccg gcgaggacgt
     2041 catcggcttc ggcgccggcg agccggactt cccgaccccg gaccacatcg tcgaggcggc
     2101 cgcggccgcc tgccgcgtgc cccggttcca ccgctacacg cccaccgccg gcctgcccga
     2161 gctgcgggag gcgatcgcgg ccaagaccgc ccgcgactcg ggctacgccg tcccgcctgg
     2221 ccaggtgctc gtcaccaacg gcggcaagca ggcgatcttc gaggcgttcg cgaccctgct
     2281 cgacccgggc gacgaggtgc tcctgccggc gccgtactgg gtcacctacc cggaggcgat
     2341 caccctggcc ggcggcgtgc cggtggtggt cccgaccgag gccgcgaccg ggttccgggc
     2401 gaccgtcgag cagctcgagg ccgcgcgcac gccgaggacc aaggtcctgc tgttcgtctc
     2461 gccgtcgaac ccgaccgggg cggtctaccc gcgggccgag atggaggcgg tcggccgctg
     2521 ggcggccgac cggggcctgt gggtcgtgac cgacgagatc tacgagcacc tcgtctacgg
     2581 cgacagccgg ttccactccc tgccggtgct ggtccccgag ctcgccgacc ggtgcgtcgt
     2641 ggtcaacggg gtggccaaga cgtacgcgat gaccgggtgg cgggtcggct ggatgctcgc
     2701 cccgcccgac gtcgtcgcgg ccgcgacgaa cctccagagc cacgcgacct ccaacgtggc
     2761 caacgtcgcc caggccgcgg ccctggccgc ggtctccggc gacctgtccg acgcggcccg
     2821 gatgcgggcg gccttcgacc gccgtcggcg gcggatccac gagctgctca cggccatccc
     2881 aggggtggcg tgcatggagc cgcagggcgc cttctacgcc ttcccctcgt tcgcccggat
     2941 gctcggccgc gagctgcgcg gccgcacgcc cgcgagcacg ctcgagctcg ccgaggtcat
     3001 cctcgacgag gcgaaggtgg cgatcgtgcc aggggaggcg ttcggcgcgc ccggctacgc
     3061 gcgcctgtcc tacgcgctcg gcgacgacga cctggtcgag ggcatccgcc gcgtcgagct
     3121 gctcgacgga ggccaggtac ccggtggact cgtcggtcgg cacggtcacc ggcacgccgc
     3181 ccgccagcgc gatcgcctcc ggataggtgg tc
//
LOCUS       DASPYY010000408         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1614695, whole genome shotgun sequence.
ACCESSION   DASPYY010000408 DASPYY010000000
VERSION     DASPYY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614695"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VEP73_06335"
     CDS             complement(<1..704)
                     /locus_tag="VEP73_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYT26081.1"
                     /translation="MPELPELDVLTQNLATNLAGASVAAVRLYSVAALKTFDPPLDEL
                     AGRVVTGAGRRAKYVWLELSGWYLVVHLSLGGRISLATKPPSRKGGVLALELADGRVL
                     NVTEGGTQRRAAIWLVDDPEKVPGVAGLGPEPLDPGFTVEVLGAILAAGGHTLKGMLT
                     DQRGMSGVGNAWSDDVLHRARLSPFARPDRLSPEEVARLHASLVGVLGEARGALAEQV
                     RGEVVIPKAAARQFAV"
     gene            complement(711..1541)
                     /gene="rnc"
                     /locus_tag="VEP73_06340"
     CDS             complement(711..1541)
                     /gene="rnc"
                     /locus_tag="VEP73_06340"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYT26082.1"
                     /translation="MERALGVRFGNEELYVWAFTHRSYAYENGGLPTNERLEFLGDAV
                     LGLVVTDIIYRAFPNLPEGQLAKLRAATVNMNVLADVARELGVGAAVRLGRGEELSGG
                     RDKSSILADTLEAILGAVYLDKGLARAAALVRRLFEGRVMEAAGRGAALDYKTSLQEL
                     AASSLGGMPSYAIEEEGPDHAKRFTATVSVGGSAYGTGRGRSKKEAEQHAAQEAFESL
                     VAERMVTGTGDSGEAEVLPATAQPPPAVDAAANGQGGGPGGRAGSEPGVHPADGPATG
                     "
     gene            complement(1552..1728)
                     /gene="rpmF"
                     /locus_tag="VEP73_06345"
     CDS             complement(1552..1728)
                     /gene="rpmF"
                     /locus_tag="VEP73_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYT26083.1"
                     /translation="MPVPKRKTPRSTTRARRSQWKSRTPTYAACPQCRSAKLPHAVCK
                     NCGYYGGRQVVEVD"
     gene            complement(1715..2323)
                     /locus_tag="VEP73_06350"
     CDS             complement(1715..2323)
                     /locus_tag="VEP73_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HYT26084.1"
                     /translation="MSKQTTAPEFVVDVRELVSRPGAHKHVVLEAPLPELATPVARVA
                     DGTPVHVDVEIESVVEGLLVTGEVATRATLQCARCLCEFSDDLTVGVRELFALDPAGA
                     EEEGYALLRDDRLPLDTMVRDAVVLAFPSAPLHSADCAGLCTVCGADRNLVDCGHGRQ
                     AVDPRWAALAGLGAQLDTADPTSQGPPAGPEPHPEEEDDARP"
     gene            complement(2531..3004)
                     /gene="coaD"
                     /locus_tag="VEP73_06355"
     CDS             complement(2531..3004)
                     /gene="coaD"
                     /locus_tag="VEP73_06355"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYT26085.1"
                     /translation="MRTACCPGSYDPATNGHLDIVARAAACFDRVVVAVLTNPSKTAL
                     FSLEERLAMLKEATAGIPGVEVDSFSGLLVEYARARGVTAIVKGLRAVSDFDYELQMA
                     QMNYRLAGVETLFMSTNPSYSYLSSSLVKEVARHGGDVSGLVPDFVVARLRDRAG"
     gene            complement(3131..>3425)
                     /locus_tag="VEP73_06360"
     CDS             complement(3131..>3425)
                     /locus_tag="VEP73_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HYT26086.1"
                     /translation="TVRRTKAETFLRAGGAGPFDLVFLDPPYAQGVGVLAGLLAGLAR
                     GALAPGATVVVESAARAEPPPWPPGLEPGRPRRYGDTALHPATFRPAAGAPAR"
BASE COUNT          455 a         1214 c         1295 g          461 t
ORIGIN      
        1 tggaccgcga actggcgggc ggccgccttg gggatgacca cttcgccgcg gacctgctcg
       61 gcgagggcgc ccctggcctc gccgagcacg cccaccaggc tggcgtggag ccgggcgacc
      121 tcctccgggc tgagccggtc gggccgggcg aacggcgaca gccgcgcacg gtgcaggacg
      181 tcgtcgctcc aggcgttgcc gaccccggac atccctctct ggtcggtgag catgcccttg
      241 agcgtgtggc cgccggctgc gaggatcgcg ccgagcacct cgacggtgaa gcccgggtcg
      301 agcggctcgg gacccaggcc ggccacgcca gggaccttct ccgggtcgtc caccagccag
      361 atcgcggccc tccgctgggt accgccctcg gtgacgttga gcacgcggcc gtcggcgagc
      421 tcgagggcga gcaccccgcc cttgcgggac ggcggcttcg tcgcgagcga gatgcggcca
      481 ccgagggaca ggtggacgac caggtaccaa ccggacagct cgagccagac gtacttggcc
      541 ctgcggccgg cacccgtcac caccctcccg gccagctcgt ccagaggcgg gtcgaaggtc
      601 ttcagcgcgg cgaccgagta cagccgcacc gccgcgaccg aggccccggc caggttcgtg
      661 gccaggttct gggtgagcac gtcgagctcg gggagctcgg gcacctcgac ctacccggtg
      721 gccggtccgt cggccgggtg aacccccggc tcgctcccgg cccggccgcc cggcccgccg
      781 ccctggccgt tcgccgcggc gtcgacggcc ggcggcggct gcgcggtggc cggcagcacc
      841 tcggcctcgc cgctgtcgcc ggtgccggtc accatgcgct cggcgaccag cgactcgaac
      901 gcctcctggg cggcgtgctg ctcggcctcc ttcttggagc ggccccgccc ggtgccgtag
      961 gcgctcccgc ccaccgacac ggtggcggtg aagcgcttgg cgtggtcggg gccttcctcc
     1021 tcgatggcgt acgagggcat gccgccgagg ctggacgccg ccagctcctg cagcgaggtc
     1081 ttgtagtcga gggcggcgcc ccggccggcc gcctccatga cccgcccctc gaagaggcgg
     1141 cggaccagcg ccgcggcccg ggccaggccc ttgtcgaggt agacggcccc gaggatcgcc
     1201 tcgagggtgt cggccaggat cgacgacttg tcgcggccac ccgacagctc ctcgccccgt
     1261 ccgaggcgga ccgccgcacc gaccccgagc tccctggcca cgtcggcgag cacgttcatg
     1321 ttgaccgtgg ccgcccgcag cttggccagc tgaccttcag ggaggttggg gaaggcgcgg
     1381 tagatgatgt cggtgaccac gaggccgagg acggcgtcgc cgaggaactc gagccgctcg
     1441 ttggttggca ggccgccgtt ctcgtaggcg taggaccggt gcgtgaatgc ccagacgtag
     1501 agctcctcgt tgccgaagcg gacaccgagc gccctctcga tcggctgccg cttagtcaac
     1561 ctcgacgacc tggcgaccac cgtagtaccc acagttcttg cagacggcgt gcggcagctt
     1621 cgcggagcgg cactggggac aggcggcgta ggtcggtgtc ctgctcttcc actgggagcg
     1681 ccgcgctcgg gtcgtcgacc gcggcgtctt gcgcttaggg acgggcatcg tcttcctcct
     1741 cggggtgcgg ttcggggccg gcgggcggcc cttgggaagt ggggtcggcg gtgtcgagct
     1801 gcgcgcccag cccggcgagc gcggcccagc gggggtccac ggcctggcgc ccgtgaccgc
     1861 agtcgaccag gttgcggtcg gcgccgcaga ccgtgcagag cccggcgcag tcggcgctgt
     1921 gcagcggagc ggacggaaag gcgagcacca cggcatccct gaccatggtg tcgagcggca
     1981 gccggtcgtc gcgcagcagg gcatagccct cctcctcggc gccggccggg tcgagggcga
     2041 acagctcgcg gacgccgacc gtgaggtcgt cggagaactc gcacaggcag cgggcacact
     2101 ggagggtcgc ccgggtggcc acctcgccgg tgacgagcag gccctcgacg acgctctcga
     2161 tctcgacgtc gacgtggacg ggcgtgccgt cggcgacccg ggcgaccgga gtggcgagct
     2221 cgggcagcgg ggcctcgagg accacgtgct tgtgcgctcc cggacggctg acgagctcgc
     2281 ggacgtccac gacgaactca ggggcggtgg tctgcttgga catggcgaaa ctggactctt
     2341 acgtgtgctg acggggtacg tcccaattcg atgccgaccc ggtactggtt cgataccgtc
     2401 ccgataccgg atggctcgat gccgaaggac gcataggcct gccggcggca ggccaccggc
     2461 gcagcgtagc agaggagcgg gcgggtggtc gaacatggct cgaccgcggc ctcgaccgtg
     2521 cccggtggcc tcagccggca cggtctcgca gcctcgccac cacgaagtcg ggcaccaggc
     2581 cgctgacgtc gcccccgtgc cgcgcgacct ccttgaccag gctcgaactg aggtacgagt
     2641 aggaggggtt cgtgctcatg aagagcgtct ccaccccggc gaggcggtag ttcatctgcg
     2701 ccatctgcag ctcgtagtcg aagtcgctga ccgcccgcag ccccttgacg atagcggtca
     2761 ccccgcgggc gcgggcgtat tcgacgagca ggccggagaa ggagtccacc tcgacgccgg
     2821 ggatcccggc ggtcgcctcc ttcagcatgg cgaggcgctc ctcgagcgag aacagggccg
     2881 tcttggacgg gttggtgagc accgcgacga ccacccggtc gaagcaggcc gccgctctgg
     2941 cgacgatgtc gagatgaccg ttggtcgccg ggtcatagct gcccgggcag catgcggtcc
     3001 gcacgtggag tctcctgcct tccgtggtct cctgccttcc gtggtccaag gtccgggtcc
     3061 aggtcgcgcc ctgccgccag gcggcgggga cgaccagggt aaccggtccg gcggacgcgg
     3121 cggcgggccg tcaccgcgca ggcgctccgg ccgccggcct gaaggtggcc gggtgcaggg
     3181 cggtgtcgcc gtagcggcgg ggccggccgg gctcgagccc ggggggccag ggtggcggct
     3241 cggcgcgggc ggccgactcg acgacgacgg tggcgcccgg ggcgagcgcg ccgcgggcga
     3301 ggccggccag caggccggcc agcacgccga cgccctgggc gtagggaggg tcgaggaaga
     3361 cgaggtcgaa gggcccggca ccgcccgcgc gcaggaaggt ctcggccttg gtgcgccgga
     3421 ccgtg
//
LOCUS       DASPYY010000409         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2036653, whole genome shotgun sequence.
ACCESSION   DASPYY010000409 DASPYY010000000
VERSION     DASPYY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036653"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VEP73_06365"
     CDS             complement(<1..696)
                     /locus_tag="VEP73_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT26087.1"
                     /translation="MQELASQLLIDRLVDWGIDTVFGLPGGGADGVAGALRRRADRIR
                     LVLVHHEEAAAFMAAGYAKVTGRLGVCLATSGPGAIRLLSGLYDAKLDHQPVLAVTEL
                     PDPQLLGTSIAQELRLDQVFEDVADCNVSVDVPVQIPAVVDIAVGHAMARSTVSHITF
                     PHEMKPGIDVSYWVSTTLSVPATAPVFVPAPGVPPLDELGRAAAVLNEGTRRAVLVGV
                     GGFEARAELLAVAD"
     gene            complement(747..1178)
                     /gene="dhaM"
                     /locus_tag="VEP73_06370"
     CDS             complement(747..1178)
                     /gene="dhaM"
                     /locus_tag="VEP73_06370"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006173110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HYT26088.1"
                     /translation="MSVGLVVVSHSAKVAEGVAELAAEMAGEVKVMAAGGADGGEIGT
                     SPPRIAAAIEAADDGDGVLVLVDLGSAVLAAQMAIEELVAEERRGRVRISEGPVVEGT
                     VIGAIQASTGAGLDAVDRAAAGAAVMAVMREDGNEDEREKP"
     gene            complement(1175..1834)
                     /gene="dhaL"
                     /locus_tag="VEP73_06375"
     CDS             complement(1175..1834)
                     /gene="dhaL"
                     /locus_tag="VEP73_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HYT26089.1"
                     /translation="MTGLERSGDVFSSGQAIAWVRGTAAVIEERAPELTRLDAAIGDG
                     DHGTNMARGFRNVLVRLEGLSRQDLGSVFKEVSMALIGKVGGASGPLYGSFYLAMGRE
                     LGEATEVEDARLAAGLRAGYDAVVARGKAQLGDKTMLDALHPALEALDAALADGKELG
                     GALDAAEAAAREGVAATIPLVARKGRASYLGERSRDHQDPGATSSHLIVKALADVVNG
                     R"
BASE COUNT          260 a          814 c          700 g          274 t
ORIGIN      
        1 gtcggcgacc gcgaggagct cggcccgcgc ctcgaagccg cccacgccga ccaggacggc
       61 ccgtcgggtg ccctcgttca gcacggcggc cgcccgcccg agctcgtcca ggggaggcac
      121 cccgggggcc gggacgaaga ccggggccgt ggccgggacg gacagggtcg tcgacaccca
      181 gtagctcacg tcgatgcccg gcttcatctc gtgcgggaag gtgatgtggg agacggtgct
      241 ccgggccatg gcgtgaccga cggcgatgtc gaccaccgcc gggatctgga ccggcacgtc
      301 gacgctcacg ttgcagtcgg cgacgtcctc gaacacctga tcgagcctga gctcctgggc
      361 gatgctggtg ccgaggagct gcgggtcggg cagctcggtg accgcgagca ccggctggtg
      421 gtcgagcttg gcgtcgtaga gcccgctcag caggcggatg gcgcccggtc cggaggtggc
      481 gaggcagacg ccgagcctgc cggtcacctt ggcgtacccg gccgccatga aggcggccgc
      541 ctcctcgtgg tggacgagga cgagccggat ccggtcggcg cggcgccgca gtgccccggc
      601 caccccgtcc gcgccgccgc cgggcagtcc gaagacggtg tcgatccccc agtcgacgag
      661 ccggtcgatc agcagctggg aagcgagctc ctgcaccggc cgtccccttc gcgcgcgtcc
      721 cgtcgtactg gccactgccc cgagcgtcac ggcttctcgc gctcgtcctc gttcccgtcc
      781 tcgcgcatga cggccatgac ggccgcgccg gccgcggccc ggtcgaccgc gtcgaggccg
      841 gcgccggtgc tggcctggat ggcgccgatc accgtcccct cgaccaccgg cccctccgag
      901 atccgcaccc ggccgcgccg ctcctcggcc accagctcct cgatcgccat ctgggcggcc
      961 agcacggccg acccgaggtc gaccagcacg aggacgccgt cgccgtcgtc ggccgcctcg
     1021 atggccgccg cgatcctggg aggggaggtg ccgatctcgc cgccgtcggc gcccccggcg
     1081 gccatgacct tcacctcgcc ggccatctcg gcggccagct cggcgacgcc ctcggcgacc
     1141 ttggccgagt gggagacgac cacgagaccc acgctcacct gccgttcacc acgtccgcga
     1201 gcgccttgac gatcaggtgg ctcgaggtgg cgccagggtc ctggtggtcg cgggagcgct
     1261 cccccaggta gctcgcccgg cccttgcggg cgacgagcgg gatggtcgcc gcgacgccct
     1321 cccgggcggc cgcctcggcc gcgtcgaggg cgccgcccag ctccttgccg tcggcgagcg
     1381 cggcgtcgag tgcctccagg gccgggtgca gggcgtccag catggtcttg tccccgagct
     1441 gcgccttgcc cctggcgacg accgcgtcgt agccggcccg caggccggcg gccaggcggg
     1501 cgtcctcgac ctcggtcgcc tcgcccagct ccctgcccat ggccaggtag aacgacccgt
     1561 acaggggccc ggaggcaccg cccaccttgc cgatcagcgc catgctcact tccttgaaca
     1621 ccgagcccag gtcctgcctg gagagcccct cgagccgcac gagcacgttc cggaagcccc
     1681 gcgccatgtt cgtcccatgg tccccgtccc cgatcgccgc atcaagccgc gtcagctccg
     1741 gcgcccgctc ctcgatgacc gccgccgtcc ccctgaccca ggcaatcgcc tgcccagagg
     1801 agaacacgtc gccactcctc tccagccccg tcatcgatct ccttcctccg gcccgggtga
     1861 gcccggggtg ggatggacaa aggatgccgc ggaggtggcc ccgttcgcag cgactgctct
     1921 ccgtcggagc ggagaacggg gccacctccg cggctaccac ggacgaccgg agcggcctac
     1981 tccacccacc cgcgggtccg ctccaccgcc ttcttccacc acgcgtagcc cgactcgcgc
     2041 tggtcctc
//
LOCUS       DASPYY010000410         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_632717, whole genome shotgun sequence.
ACCESSION   DASPYY010000410 DASPYY010000000
VERSION     DASPYY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632717"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..2129
                     /locus_tag="VEP73_06380"
     CDS             198..2129
                     /locus_tag="VEP73_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26090.1"
                     /translation="MRMVFGPDDEKEFFEVRDELTDRFAEWAEAHAPAADPGLAQLAL
                     DYKWGYGGGNLARWTGAELRDLLCEWFPRKVTLDEGDFDRVVPSVRAFVEYLAQGGQL
                     DPGSDRPERLRAALEGLEREFQSAMRDPSRFGMAKSFATAMMAGGVDMSDEAAVGRFM
                     NEFNALPFEARELLTGAPEAPPIALPPVWLPTGAELAGAAAAAPAVERLHRFVAWLGT
                     GRKLTQSGRLTLADGKELVGVLGTGDVVDPRIGDRTFRTRSSEELRGVNLTFAWAKAA
                     RLARVVHGRVVPNKKGQALLDRPLELWERALEGLGKLGPVVLDRGILGAFLDDSFDQL
                     IPSLLCSLYASDGPVPFGELADLVWEDVQRTYVVDQEPAQRIGFWRDMVAADVGLAVG
                     ELEQLGAVERLHPPSAPGEQAGAPPGEDGDDEEAVAPATRLQLAPLGTWAANRILRAE
                     GADAPVVGDLASADAATLLERCATYDEESCKAEMAAWCEARGPAVAAEQLAAFARTAD
                     HEGRMLALTALDAAGPAGVEAVQALREEPGLRPLATMWLLDHGLADPGDLDPETVLTV
                     MVETTAHILATEGPEAAVEAFSELAPPEEQAAVVGNLWRADSPRTAGLLDALAEAHPD
                     KAVAKAARKAAFKLRSSGR"
     gene            2156..3010
                     /locus_tag="VEP73_06385"
     CDS             2156..3010
                     /locus_tag="VEP73_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT26091.1"
                     /translation="MGARVGAVVGVLALGVVLAVVVLWSLQRRLIYYPHPARVPAAAS
                     VLPGSEEVAFDTEDGLRLRGWFVPGRGGGGAAVLVCNGNGGNRSLRAPLAAALSRAGL
                     SVLLFDYRGYGGNPGHPSEAGLAADARAARAWLAARDGVDPDRIVYLGESLGAAVALG
                     LAVERPPAALVLRSPFTSLADVGRLHYPWLPVGLLLADRYPSLDRVGRLTVPLLVVAG
                     EGDRVVPAAQSRRLYEAAPEPKRFALVPGADHNDWELLAGARLLDEVLGFLDGTAGLR
                     LGRGPSGP"
     gene            3126..4235
                     /locus_tag="VEP73_06390"
     CDS             3126..4235
                     /locus_tag="VEP73_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT26092.1"
                     /translation="MKDDVFVFDSHTGYWDASPENCKNRFGDAFIETFYAFHTGFNPP
                     DPKWTMDYERTFRKVDPDWYLEEMFVKGTADMAILSTQVLSDFYHTGFVNPERNAQLA
                     ARAPERLIPLGGVDPRAPDALEQVEHQITDLGMRGFKWYTAEWRGQSRGWAANDPMVF
                     PLYEKALELGIRNHHFHKGPAVEPLSLAKFDVRDIDEPAWLYPDMNFIVDHCGAPRVD
                     DFCWLATRCPNVYAGLAVVLALIHNRPRWFAEVMANLLYWLGPDRIVYGTDFPIWYPH
                     WQLDDFMAFELPEDLKQEYGVDLTPEIKRKIVGGNIARLYGIDVEARLQAIKDDELAR
                     RRSDYLAGQPPVEPKDVPGTEQPAVETGVAGTESR"
     gene            4232..4603
                     /locus_tag="VEP73_06395"
     CDS             4232..4603
                     /locus_tag="VEP73_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYT26093.1"
                     /translation="MSAGGRTRSPSGGERAAGVDPEQVRKVAGGVRDPEVRTPIAALG
                     LLDEVEVDGGRVTVRFHLTSPLCPAQFAGRIGQDIRRRVGRLPGVESVEVVLQDHFMA
                     DALHRLINEGDRSAVTRGLLR"
BASE COUNT          608 a         1744 c         1826 g          640 t
ORIGIN      
        1 atgcgcggcg gcggttgcac ccccggctgg ctgttggcca gcacgggcac cattcgagca
       61 tctgtctcgg gtatgcgtcg gtattgtaat cgtgtaatca gccgatcgcg gcgaccccgt
      121 ccggtcggcc gggcacgggt cgtcgggcgg gatgggccgt atcatcgcgg ggctgcagcc
      181 ggacagggga gcttcacatg cggatggtgt tcggacccga cgacgagaag gagttcttcg
      241 aggtccggga cgagctgacg gatcggttcg ccgagtgggc ggaggcgcac gcgccggcgg
      301 ccgacccggg actcgcccag ctcgcgctcg actacaagtg ggggtacggg ggcgggaacc
      361 tcgcacgctg gaccggggcg gagctccgcg acctgctgtg cgagtggttc ccccgcaagg
      421 tcacgctcga cgagggggac ttcgaccgcg tggtgccgag cgtgcgggcg ttcgtcgagt
      481 acctggcgca gggcggccag ctcgacccgg gcagcgaccg cccggagcgg ctccgggcgg
      541 cgctggaggg gctcgagcgc gagttccaga gcgcgatgcg tgacccgtcc cggttcggca
      601 tggccaagtc gttcgccacc gcgatgatgg ccggcggcgt ggacatgagc gacgaagcgg
      661 cggttggccg gttcatgaac gagttcaacg ccctcccgtt cgaggcgcga gagctgctca
      721 cgggtgcgcc cgaggccccg ccgatcgcgc tcccgccggt ctggctcccg accggggccg
      781 agctcgccgg ggccgccgcg gcggccccgg ccgtcgagcg gctccaccgg ttcgtggcct
      841 ggctcggcac cgggcgcaag ctcacccagt ccggcaggct gacgctggcc gacggcaagg
      901 agctggtcgg ggtgctcggc accggcgacg tcgtcgaccc ccggatcggc gaccgcacct
      961 tccgaacccg cagctccgag gagctgcgcg gggtgaacct gacgttcgcc tgggcgaagg
     1021 ccgctcggct ggccagggtc gtccacggcc gggtggtgcc gaacaagaag ggccaggcgc
     1081 tcctggaccg cccgctggag ctgtgggagc gggcgctcga gggcctcggc aagctcggcc
     1141 cggtcgtgct cgaccgcggg atcctcggcg cgttcctcga cgacagcttc gaccagctga
     1201 tcccctcgct gctctgctcc ctctacgcga gcgacgggcc ggtcccgttc ggcgagctgg
     1261 ccgacctcgt ctgggaggac gtccagcgga cgtacgtcgt cgaccaggag ccggcccagc
     1321 gcatcgggtt ctggcgcgac atggtcgccg ccgacgtcgg cctcgccgtt ggcgagctcg
     1381 agcagctcgg cgccgtcgag cgcctccacc ctccgtccgc gcccggcgag caggcgggag
     1441 cgccgcccgg cgaggacggc gacgacgagg aagcggtggc gccggccacc cggctgcagc
     1501 tcgccccgct cggcacctgg gccgcgaacc ggatcctgcg tgccgagggg gccgacgcgc
     1561 cggtcgtcgg cgacctggcg agcgccgacg cggccaccct gctggagcgc tgcgcgacct
     1621 acgacgagga atcgtgcaag gccgagatgg cggcgtggtg cgaggcgcgc ggtcccgcgg
     1681 tcgcggcgga gcagctcgcc gcctttgcgc gcacggccga ccacgaaggg cgcatgctcg
     1741 cgctcaccgc cctggacgcc gccgggccgg cgggcgtgga ggcggtccag gcgctgcggg
     1801 aagagccggg cctgcggccg ctcgccacga tgtggctgct cgaccacggg ctcgcggacc
     1861 cgggcgacct ggatcccgag acggtgctga cggtcatggt cgagacgacc gcccacatcc
     1921 tcgcgacgga gggcccggag gccgccgtcg aggcgttctc cgagctggcc ccgcccgagg
     1981 agcaggccgc ggtcgtcggc aacctctggc gcgcggacag cccccggacc gccgggctcc
     2041 tggacgcgct ggccgaggcc catcccgaca aggccgttgc caaggcggcc cgcaaggcgg
     2101 cgttcaagct gcgctcctcc gggcgctgac cggcgggcgc ggcctgaccc tcccgatggg
     2161 cgcgcgggtg ggagccgtgg tgggcgtgct cgcgctcggg gtggtgctcg cggtcgtggt
     2221 gctctggtcc ctgcagcgcc ggctgatcta ctacccgcat ccggcgcggg tgccggccgc
     2281 cgcgagcgtc ctgccgggga gcgaggaggt cgccttcgac accgaggacg ggctgcgcct
     2341 gcggggctgg ttcgtgcccg gccgcggcgg cggcggggcg gcggtgctgg tctgcaacgg
     2401 caacggcggg aaccggtcgc tgcgggcgcc gctcgcggcg gcgctctccc gcgccggcct
     2461 ctcggtcctc ctcttcgact accgcggcta cggcgggaac cccggccacc cctcggaggc
     2521 ggggctggcc gctgacgcgc gggccgcgcg ggcgtggctc gcggcgcgcg acggcgtcga
     2581 cccggaccgg atcgtctacc tcggggagtc gctcggcgcc gcggtcgcgc tcggcctggc
     2641 ggtggagcgg ccgccggccg cgctcgtcct gcggtcgccg ttcacctccc tggccgacgt
     2701 cgggcgcctg cactacccgt ggctcccggt cggcctgctg ctcgccgacc ggtacccgtc
     2761 gctcgaccgg gtcggtcggc tcaccgtgcc gttgctggtc gtcgcggggg agggggaccg
     2821 tgtcgtcccc gcggcccaga gccgccggct ctacgaggcg gccccggagc ccaagcggtt
     2881 cgcgctcgtc ccgggcgccg accacaacga ctgggagctg ctcgccggcg cccgcctgct
     2941 cgacgaggtc ctgggcttcc tcgacggcac cgccgggctg cggctcgggc gcggcccgag
     3001 cgggccctga gccgctccgc gagagctgcc tcttgtcctg ggccgtctcg atgggcagga
     3061 tgaacaagtt cggcggaagg gcggcgggtg ggcccggagc ggcctccgca gaagggagtt
     3121 gcccgatgaa ggacgacgtc ttcgtcttcg attcccacac cggctactgg gacgcgagcc
     3181 ccgagaactg caagaaccgg ttcggcgacg cgttcatcga gaccttctac gccttccaca
     3241 ccgggttcaa cccgcccgac cccaagtgga cgatggacta cgagcgcacc ttcaggaagg
     3301 tcgaccccga ctggtacctg gaggagatgt tcgtcaaggg caccgcggac atggcgatcc
     3361 tgtccaccca ggtcctgtcg gacttctacc acaccgggtt cgtcaacccg gagcgcaacg
     3421 cccagctcgc cgcgcgggcg cccgagcggc tgatcccgct cggcggggtg gacccgcgcg
     3481 cccccgacgc gctcgagcag gtcgagcacc agatcaccga cctcggcatg cgtggcttca
     3541 agtggtacac ggccgagtgg cgcggccagt cccggggctg ggcggccaac gacccgatgg
     3601 tcttcccgct gtacgagaag gccctcgagc tcggcatccg caaccaccac ttccacaagg
     3661 gcccggcggt ggagccgctg tcgctggcga agttcgacgt gcgcgacatc gacgagccgg
     3721 cctggctgta cccggacatg aacttcatcg tcgaccactg cggcgcgccc agggtcgacg
     3781 acttctgctg gctcgcgacg cgctgcccga acgtgtacgc cggcctcgcg gtcgtcttgg
     3841 cgctcatcca caaccggccg cggtggttcg ccgaggtgat ggccaacctg ctgtactggc
     3901 tcggcccgga ccggatcgtg tacggcaccg acttcccgat ctggtacccg cactggcagc
     3961 tggacgactt catggcgttc gagctccccg aggacctcaa gcaggagtac ggcgtcgacc
     4021 tgaccccgga gatcaagcgg aagatcgtcg gcggcaacat cgcccgcctg tacggcatcg
     4081 acgtggaggc gaggctccag gcgatcaagg acgacgagct cgcccgccgc cgcagcgact
     4141 acctggccgg ccagcccccc gtcgagccga aggacgtgcc aggcaccgag cagccggcgg
     4201 tcgagaccgg cgtcgccggc accgagtccc gatgagcgcc ggcgggcgga ccaggagccc
     4261 gtcgggcggg gagagggcgg cgggggtcga ccccgagcag gtccggaagg tcgccggggg
     4321 cgtccgcgac cccgaggtgc ggaccccgat cgccgccctc ggcctgctcg acgaggtcga
     4381 ggtggacggc gggcgggtga ccgtccgctt ccacctgacc tcgccgctct gcccggcgca
     4441 gttcgccggc cggatcggcc aggacatccg gcgccgggtc ggcaggctgc ccggcgtgga
     4501 gtcggtcgag gtcgtcctgc aggaccactt catggccgac gcgctgcacc ggctcatcaa
     4561 cgagggggac cggtcggcgg tgacccgcgg gctgctgcgg tgaccggggc ggccgaccgc
     4621 ggccccggct ccggccccgg ccccgggatg ggggtggccc gatgccgctg accgaccccg
     4681 cccgcatgct cgccgggcgg cgtgaggccg cgctcctcga ggtgccgagc gtgcgccgga
     4741 tggcggacgt gctcgccgtc cgctgccggg agccgtcctg ggtgcgcacg ctcgtggcca
     4801 gcctggaccg gttcaaga
//
LOCUS       DASPYY010000411         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2739535, whole genome shotgun sequence.
ACCESSION   DASPYY010000411 DASPYY010000000
VERSION     DASPYY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2739535"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VEP73_06400"
     CDS             complement(<1..1775)
                     /locus_tag="VEP73_06400"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HYT26094.1"
                     /translation="MLYGAVPRRPYTARVVEQRTADPLSARLGAVPEVPKALARALEE
                     AFGYGTVRDLLEHYPRRYLTRGELTDLAHARKGATITVVGTVRNVAKKQPRRNLRILE
                     LRVTDENRSGVLTCTWFNQPWHAQKLPVGTLAAFSGKLEWKAGRLGMANPGYEVLRES
                     DDAEGFANEIIAVYPATKEVSSGRLRRSVAALLDRYGQVPDFLPDDLRGAHRLPGRAE
                     SLAAIHRPPDMAAKDRARDRLVYDELLVLQVGLALRRRALATGETGHALATDGDVTKR
                     LLASLPFTPTGAQSRAMAEIGADLARPVPMHRLLQGDVGSGKAQPLDALVLTPTGFTR
                     MGAIRVGDEVVNPTGEVTVVDGVYPQGERDVWRLVLSDGTGVECDDDHLWAVDTSVRR
                     HRGHPLKVMTTREIREDLTEPNGTSKWHLPMAEPVDLDMGEDRPLDPYQLGQLLGDGS
                     FRDKHVPDAYRNAPVKVRHAVLQGLLDTDGTLDSGRGGDVSFASPSERLARDVQWLVE
                     SLGGVGRLRPVTKVGGTYFMVSIRLPDDYPPFRLGRKADLFRPRTTYRHLGRAVRGVE
                     YVGRKPVQCISVAHPNHLYVTDHFVV"
     gene            1948..2139
                     /gene="rpmB"
                     /locus_tag="VEP73_06405"
     CDS             1948..2139
                     /gene="rpmB"
                     /locus_tag="VEP73_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYT26095.1"
                     /translation="MAAVCDICGKHPGFGMSVSFSHKRHPRRWNPNIQRIKILEGRTP
                     RRRNVCTSCIKANKVRRAV"
     gene            2169..2678
                     /locus_tag="VEP73_06410"
     CDS             2169..2678
                     /locus_tag="VEP73_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26096.1"
                     /translation="MQPVAVRSTGLAAPVRAVALGVVTGSRQQLAIALLAGAASRGRF
                     DPGAGRVAERLRSPGATAVAALAAAAELVADKLPFTPARTAPGPMLGRVATGGLVGAS
                     VLRDAHGSAALGAALGALGAAGGAVAGNRARVALARRTRVPDLVWGVVEDLVALGLGT
                     LAVASLPGR"
BASE COUNT          326 a          969 c         1003 g          392 t
ORIGIN      
        1 accacgaagt ggtcggtgac gtacaggtgg ttcgggtgcg cgacggagat gcactgcacc
       61 ggcttccgcc cgacgtactc gaccccccgg accgcccgcc cgaggtgccg gtacgtggtc
      121 cttggcctga acaggtcggc cttccggccg agccggaagg gcgggtagtc gtccggcagc
      181 cggatgctga ccatgaagta ggtgccgccc accttcgtga ccgggcggag ccggcccact
      241 cccccgaggg actcgacgag ccactgcacg tcccgggcga gccgctccga gggcgaggcg
      301 aaggagacgt cgcctccccg cccgctgtcg agcgttccgt ccgtgtcgag aagcccttgg
      361 aggacggcgt ggcggacctt gaccggcgcg ttccggtagg cgtcagggac gtgcttgtcg
      421 cggaacgagc catccccgag cagctgtccc agctggtatg ggtcgagggg gcggtcctcc
      481 cccatgtcga ggtccacggg ctccgccatc ggcaggtgcc acttggacgt cccgttcggc
      541 tccgtgaggt cctctctgat ctcgcgggtc gtcatcacct tcaagggatg gccgcgatgg
      601 cgccgcacgg aggtgtcgac tgcccacagg tggtcgtcgt cgcactcgac gccggtgccg
      661 tcggagagca cgagtctcca tacgtcgcgc tcaccctgcg gatagacgcc gtccaccacc
      721 gtgacctcgc ccgtcgggtt gaccacctcg tcgccgacac gaatagcacc catacgggtg
      781 aagccggtcg gggtgagcac gagggcgtcg agcggctgcg ccttgccgct gccgacgtcc
      841 ccctggagga ggcggtgcat cggcaccggg cgggcgaggt cggcgccgat ctcggccatc
      901 gcccgggact gggcgccggt cggcgtgaac ggcaggctgg cgagcagccg cttggtgacg
      961 tcgccgtcgg tggccagcgc atgaccggtc tcccccgtcg cgagcgcgcg gcggcgcagg
     1021 gccaggccga cctggagcac gagcagctcg tcgtagacga gccggtcccg ggcacggtcc
     1081 ttggccgcca tgtcgggcgg gcggtggatc gccgccagcg actcggcgcg cccggggagg
     1141 cggtgggcgc cgcgcaggtc gtcgggcagg aagtccggca cctggccgta gcggtccagc
     1201 agcgcggcga cggaccggcg gagccggccc gacgacacct ccttcgtcgc cgggtagacg
     1261 gcgatgatct cgttcgcgaa gccctcggcg tcgtcgctct cgcgcagcac ctcgtagccc
     1321 gggttcgcca tcccgagccg gccggccttc cactcgagct tgcccgagaa ggcggccagg
     1381 gttccgaccg gcagcttctg ggcgtgccag ggctggttga accaggtgca ggtgagcacg
     1441 ccgctgcggt tctcgtcggt gacccggagc tcgaggatgc gcaggttgcg ccggggctgc
     1501 ttcttggcga cgttcctgac cgtcccgacg acggtgatcg tcgcgccctt cctggcgtgg
     1561 gccaggtcgg tcagctcgcc gcgggtgagg tagcggcggg ggtagtgctc gagcaggtcg
     1621 cggacggtgc cgtagccgaa cgcctcctcg agcgcgcggg ccagcgcctt gggcacctcg
     1681 ggcacggcgc ccagccgggc gctgaggggg tcggccgtcc tctgctccac cacgcgggca
     1741 gtataggggc ggcgggggac agcaccgtac agcaccgtct gccgccgggg gcgcgtcgcc
     1801 tcgccgcccc ggagcgttgc ccgttttgtt gcccggcgcc gaggggccgt gctaccgttt
     1861 cgcggctcgg cttctccgca gcatcacgtc gcgttcgcag catcacgtcg cggcacaggt
     1921 cgaatttcac ttcaggaagg gctctcaatg gctgccgtct gtgacatctg cggcaagcac
     1981 cccgggttcg gcatgtcggt gagcttctcc cacaagcgcc acccacgccg ctggaacccg
     2041 aacatccagc ggatcaagat cctcgagggg cgcacgccgc ggcgcaggaa cgtctgcacg
     2101 tcctgcatca aggccaacaa ggtgcggcgc gccgtctgac caggcggccg cgtccgggca
     2161 cgacgcagat gcagcccgtc gccgtccgct cgaccggcct ggccgcgccc gtgcgcgcgg
     2221 tggccctcgg cgtggtgacc ggctcgcgcc agcagctcgc gatcgccctg ctcgccggtg
     2281 cggcgagccg tggccgcttc gacccgggcg ccgggcgggt cgcggagcgg ttgcgctcgc
     2341 caggggcgac cgccgtcgcg gcgctcgccg cggccgccga gctggtcgcc gacaagctgc
     2401 cgttcacgcc ggcgcggacc gccccgggac cgatgctggg gcgcgtcgcc accggtgggc
     2461 tggtgggcgc ctcggttctc cgcgacgccc acggctccgc ggcgctcggg gccgcgctcg
     2521 gcgcgctcgg cgcggccggc ggcgcggtcg ccggcaaccg tgcgcgcgtc gccctcgcca
     2581 ggcggaccag ggtgcccgac ctggtctggg gcgtggtcga ggacctcgtc gccctcggtc
     2641 tcggcacgct cgcggtcgcg tccctgcccg gcaggtaggc gtcccgttgc
//
LOCUS       DASPYY010000412         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2598437, whole genome shotgun sequence.
ACCESSION   DASPYY010000412 DASPYY010000000
VERSION     DASPYY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598437"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..527
                     /locus_tag="VEP73_06415"
     CDS             99..527
                     /locus_tag="VEP73_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT26097.1"
                     /translation="MDDEGMRPYALRAGEGWTYRDGIDFTVKRGELGRGRRLAVIEYT
                     TRPGEEPPDHTHTTEDEIFYVLRGALTFRCGDDAFEVDDGGFVFLPRGVPHGYTIRSD
                     DVHLLVVTAPAQEDAGGGWGGFVADLEAAGELRATPPGSG"
     gene            complement(564..1451)
                     /locus_tag="VEP73_06420"
     CDS             complement(564..1451)
                     /locus_tag="VEP73_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYT26098.1"
                     /translation="MSWLGGTFSGGFAQRALLEAVMVGALCGVVGVHVLLRRLPFFTM
                     ALGHATFPGVVLAALAGIDLFLGAGLFGAAVVALVAVLGARDRVDDTSATGVVLAGTF
                     ALGVLLLSAQPGFSKDLTAYLVGSIVTVQPGDLATTAVAGAAVLVVLAALHKELVLGA
                     FDRAGLAALGYPVLALDVALLLLVEITLVTSIPAVGTILAVALIVAPAAAARLWTDRL
                     GPTLVLAPAFGVASGVIGLAISQHWRVAAGATIVLAAAAFFVLSLLFAPGHGLVASLR
                     ARRGRHVPEAHEGGPRLAA"
     gene            complement(1448..2296)
                     /locus_tag="VEP73_06425"
     CDS             complement(1448..2296)
                     /locus_tag="VEP73_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYT26099.1"
                     /translation="MSGLLVDPFRLPYMERALLEVLLLGALAGVAGVFVVLRRLAFVT
                     DALTHTVFPGIVIAAALGQSVLLGALAFGVLTAVLLALLAANRRVGVDAALAILLTSF
                     FSVGVVIVSRSRTYTADLTVFLFGRVLAVDRAELVQTLVLAVVVLAAFAALRKELLLR
                     AFDPEGAAAMGYRTVVLDLALNLLVALVVVAAVKAVGTVLVIALIVVPAAAARLVADR
                     VATMTAVACALGALGGWLGLAISYEVSVGHGVRLASGATIVVVLVALFLAAASVAPLR
                     RLAARR"
BASE COUNT          414 a          841 c          947 g          230 t
ORIGIN      
        1 cgctgccaca ccccgcccac ctcaacccct gagcaggact ttttcatggg gaagtagcta
       61 ggacggctgg ggacgcccgg acagtgaagg aggaaccgat ggacgacgaa gggatgcgcc
      121 cgtacgcgct gagggcggga gagggctgga cctaccgcga cggcatcgac ttcaccgtca
      181 agagaggtga gcttggccgg ggccggcgtc tggcggtcat cgagtacacc actcgccccg
      241 gtgaagagcc gccggaccac acccacacga cggaagacga gatcttctac gtgttgcggg
      301 gtgctctcac cttccggtgc ggagatgacg cgttcgaggt ggacgacggc gggttcgtgt
      361 tcctgccccg tggcgtcccg catggctaca cgatccgcag cgacgacgtc cacctgctgg
      421 tcgtgacggc acccgcccag gaagacgccg gcggcggctg gggcggcttc gtcgccgacc
      481 tcgaagccgc gggcgaactg cgcgccacgc cacccgggtc cggatgatgc cgtcgtcagc
      541 tcgcctgctg gccaggctcg ggctcaggcg gcgagccggg gcccgccctc gtgcgcctcg
      601 gggacgtgcc gcccgcggcg ggcgcggagc gaggcgacca gcccgtggcc cggggcgaag
      661 agcagggaca ggacgaagaa ggcggcggcg gcgaggacga tggtggcccc ggcggcgacc
      721 cgccagtgct gcgagatggc gagcccgatc acgccgctcg cgaccccgaa ggcgggggcg
      781 agcacgagcg tcggcccgag acggtccgtc cacaggcggg cggcggccgc cggggccacg
      841 atgagggcga ccgccaggat cgtgccgacg gcggggatcg aggtgaccag cgtgatctcg
      901 acgagcagca ggagcgcgac gtcgagggcg agcaccgggt agccgagggc ggccagcccg
      961 gcgcggtcga aggcgccgag caccagctcc ttgtggagcg cggcgaggac gaccagcacg
     1021 gcggcgccgg cgacggcggt ggtggcgagg tcgcccggct gcacggtcac gatcgagccg
     1081 accaggtagg cggtgaggtc cttggagaac ccgggctgcg ccgagagcag cagcacgccg
     1141 agggcgaacg tcccggccag gaccacgccc gtggcgctcg tgtcgtcgac ccggtcccgg
     1201 gcgccgagca cggcgacgag ggcgacgacg gcggcgccga acagcccggc gccgaggaac
     1261 aggtcgatcc cggcgagcgc ggcgagcacc accccaggga aggtcgcgtg ccccagcgcc
     1321 atcgtgaaga acggcagccg gcgcagcagc acgtgcaccc ccaccacccc gcagagcgcg
     1381 cccaccatga ccgcctcgag cagcgcccgc tgcgcgaagc cgcccgagaa cgtgccgccg
     1441 agccagctca tcttcttgcg gcaaggcgcc gcaggggtgc gaccgaggcg gcggccagga
     1501 agagggcgac gagcacgacg acgatcgtgg cgcccgaggc gagccggacg ccgtggccga
     1561 ccgacacctc gtagctgatc gccaggccga gccacccgcc gagggcgccg agcgcgcagg
     1621 cgaccgcggt catcgtcgcg acccggtcgg cgaccaggcg ggcggctgcg gcggggacga
     1681 cgatcagcgc gatcaccagc acggtgccga ccgccttcac ggcggccacc acgacgaggg
     1741 cgacgagcag gttgagcgcg aggtcgagga cgaccgtccg gtagcccatg gctgcggcgc
     1801 cctccgggtc gaaggcgcgc agcagcagct ccttgcgcag cgcggcgaac gcggccagca
     1861 cgacgaccgc gagcacgagg gtctgcacca gctcggctcg gtcgacggcg agcacccggc
     1921 cgaacaggaa caccgtgagg tcggcggtgt aggtgcggct gcgcgagacg atcacgaccc
     1981 cgaccgagaa gaacgaggtg agcaggatcg cgagggcggc gtcgaccccg acccgccggt
     2041 tggcggccag gagggcgagc agcaccgccg tgagcacgcc gaaggcgagc gcgccgagca
     2101 ggaccgactg cccgagcgcc gcggcgatga cgatcccggg gaagacggtg tgggtgaggg
     2161 cgtcggtgac gaaggccagc cggcgcagca ccacgaacac gcccgcgacg ccggcgagcg
     2221 cgccgagcag cagcacctcg agcagggcac gctccatgta cgggaggcgg aacgggtcga
     2281 ccaggagccc gctcatggcc gcgccacgat gacgccgtcg ccgcggagct cgagcgcctg
     2341 gccgccgtag gtgacccgga gcagctccgg ggtgagcgtg gtgccgatgg gcccgaaccc
     2401 gatctggtgg tggttcagca ggcagacctc gt
//
LOCUS       DASPYY010000413         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1755965, whole genome shotgun sequence.
ACCESSION   DASPYY010000413 DASPYY010000000
VERSION     DASPYY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755965"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEP73_06430"
     CDS             <1..325
                     /locus_tag="VEP73_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26100.1"
                     /translation="VVEPGLRSDMEDRWRAAYQRAGSGDLEALRAADPELVAEDSASL
                     GAALGRVLERLGEGERALAVGHSPTNEAAVLGLTGQVGAPLAKGAGVLVVADGERFQV
                     EPLVP"
     gene            complement(425..625)
                     /locus_tag="VEP73_06435"
     CDS             complement(425..625)
                     /locus_tag="VEP73_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018733578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HYT26101.1"
                     /translation="MSSHDVTVAGYLLVASAGVGVELLSSREGSRIPTLGTLLSRVMR
                     TRSGRVGVIAGWAWIGVHFFAR"
     gene            complement(622..1368)
                     /locus_tag="VEP73_06440"
     CDS             complement(622..1368)
                     /locus_tag="VEP73_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26102.1"
                     /translation="MAGADPGGSLRRERPTPPREPLLGGRRSRPAALASAVLLALRDP
                     VVVVFLLAAIFDVLSGDALFDGMALFAVAASLGWDRVGSRRASLGGPDAAAPAEGGRA
                     KSEHAIPVAPAPDGRVGVNLRLSPRLVMGGLAYATVVGWFSRFSLPASIAVLVPGTAA
                     VAVAWRGSPRGDLDRGRVDPFGAAVWASVFVALALWELAALLLQPSLTTDSFAHPTIS
                     VLMDPVLASHLGRSVAISLWLASGWFLLRR"
BASE COUNT          232 a          604 c          597 g          161 t
ORIGIN      
        1 ggtcgtcgag ccgggactcc gctccgacat ggaggaccgc tggcgggccg cctaccagcg
       61 ggccggcagc ggcgacctgg aggcgctgcg ggccgccgac ccggagctgg tcgccgagga
      121 ctcggccagc ctgggagccg cgctcggccg ggtgctcgag cggctgggcg agggggagcg
      181 ggcgctggcc gtcgggcaca gcccgaccaa cgaggcggcg gtgctcgggc tcaccggcca
      241 ggtcggcgcg ccgctggcca agggggccgg cgtgctggtc gtggccgacg gggagcggtt
      301 ccaggtcgag cccctggtcc cctgaccgtg cggtccaggt cgtgcgggtg gccgcccatc
      361 ccctggccac ccaccaggcc ctgcgtccgt ctcggcaggg gcccagtcga ggggagccgg
      421 tcgctcaccg ggcgaagaag tggacgccga tccaggccca gccggcgatg acgcccaccc
      481 gacccgagcg ggtgcgcatg accctgctga gcagcgtgcc aagcgtcggg atccgggagc
      541 cctcgcgaga gctgagcagc tccacgccaa cccctgccga tgcgacgagc aggtaccccg
      601 cgaccgtcac gtcatgactg ctcatcgtcg cagcaggaac cagcccgacg cgagccagag
      661 cgagatggcg accgaccggc cgaggtggga ggcgaggacc gggtccatca ggacgctgat
      721 cgtggggtgc gcgaaggagt cggtggtgag cgacggctgc agcagcaggg cggcgagctc
      781 ccacagcgcg agcgccacga agaccgaggc ccacacggcc gccccgaagg ggtcgacccg
      841 cccacggtcc aggtcgccgc gcggcgaccc gcgccaggcg accgcaacgg cggccgttcc
      901 cggcacgagc acggcgatgc tcgccggcaa ggagaagcgg ctgaaccacc cgacgaccgt
      961 ggcgtaggcg aggccgccca tcaccagcct cggggacagc cgcaggttca cgcctacccg
     1021 gccgtcgggc gccggcgcta cggggatcgc gtgttcgctc ttggcccggc ccccttcggc
     1081 gggtgcggcg gcgtcggggc cgccgagcga tgcgcgccgg ctgccgaccc tgtcccagcc
     1141 gagcgacgcg gccacggcga agagggccat cccgtcgaac agcgcgtctc cggagagcac
     1201 gtcgaagatc gcggccagca ggaacaccac gaccaccgga tcgcggaggg cgagaaggac
     1261 ggccgaagcg agcgcggccg gccgggagcg gcggccaccc aggaggggct cgcgcggagg
     1321 cgtcgggcgc tcccgccgca gggaaccgcc ggggtcagct ccggccatct ggggtctcgt
     1381 ccacgctcgc ggctccgttc gtccgcggct gcgcctgtca gggccaaccc gctcaccctg
     1441 aaccagccta cctgaaccca gcctacgcct cgcgctcagt ctctcccgga ccttgcggcc
     1501 gtcccaaccc tgctggcctc ccgcaacgcc cgggcgcccg ccgccgggcc gtcgggatgc
     1561 ccgaacaccg ccgtccccgc caccagcacc gtcg
//
LOCUS       DASPYY010000414         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2598564, whole genome shotgun sequence.
ACCESSION   DASPYY010000414 DASPYY010000000
VERSION     DASPYY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598564"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VEP73_06445"
     CDS             complement(<1..1216)
                     /locus_tag="VEP73_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26103.1"
                     /translation="MAVVEIAIGVSSRRWAQDLFRYVADHGGAVVRRRVLSEEDALEE
                     DYAVLVVDDTAGFLTPRIVGELHRRGRRVLAVHAAGDYRAPEALRGTGVDATVEDNAP
                     PERIVDLLADLAPAGGPADPAIVDDAFARITRELGDDLPPGGEPPERRDPGRGWVTVV
                     LAVTGGCGATEVALALTVACERRGERTVLVDADEVAPCLAQRVGLPLVPNVRNAVDAV
                     ERRVGSLEESLSPLPGDGPEVLCGLTSPGDWTNVRPGQVADVVSELARVCRQVIVNAG
                     PRAEDVTAVNGLARYGITRLLLTVADQVVLVGAPTPVGVARLLDWVVRHRAVDQDRPI
                     HVVLNRAPGGGYRRGELMREVFRSFMPASLHVLPADRRVEEAAWDGRLVREGPFTRAV
                     AELADELIGDTKD"
     gene            complement(1219..1863)
                     /locus_tag="VEP73_06450"
     CDS             complement(1219..1863)
                     /locus_tag="VEP73_06450"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HYT26104.1"
                     /translation="MSLPHLVTLLAGLLAMLLVLVVLRGGDVTYRVAVAAREVRAGAP
                     LARSALRFTDLRAPGAIREQLIAPDGVAGLQGWVATRTIRPGDLVTRGDFRPPAAAAQ
                     QRAMSVPVDPAHAVGGALEAGDRVDVVRVLDNGQAVFVVAGAQVLGVNRPDPSAIGPA
                     GAYSLTLAVSSQDALRLAAAIRGEKFEVVRSTGAGRVVPGPGAGLTTAGSPGTG"
     gene            2349..3590
                     /locus_tag="VEP73_06455"
     CDS             2349..3590
                     /locus_tag="VEP73_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26105.1"
                     /translation="MPEPATPPTGADRAPPLLSRLAALIGPVHERYKLAVLGALARQP
                     GGGWRLADAQDAVPWLDPESTARVLGELSGSGLLVPSTEAGTWRLSDDAQLIAAVCSA
                     LSVPRVEHERVVKVLAATVALARAAGAPDEAAFGPFLAATSVLEGDLDAIERRIDEGR
                     EEPLLEAAATARTHAADMAALLQQHAELFTGAGGLADAWERAVAVLGRLETLTDEVVA
                     VLSGQADDKVRPGPAADRGALRRIVAVADLAALASLLGVSRLPPALTPVHPSAALAAL
                     DEHLGRPDPRPVPIPEPVALPVEELDDIPDLATLAAQALEWLAGRGEAELAEWVVGGS
                     WAEATGRMAAVVEAWSRHGPCGDGTLAAELAARPELDLVGHDEVGAVSRTLVRRPAGG
                     GSHPEPARGARTPSPDGGLAP"
BASE COUNT          435 a         1432 c         1405 g          432 t
ORIGIN      
        1 catccttggt gtcgccgatc agctcgtcgg cgagctcggc gaccgccctg gtgaacggcc
       61 cttcacggac gagccgcccg tcccaggccg cctcctccac gcgcctgtcg gccgggagga
      121 cgtgcaggga cgccggcatg aacgaccgga acacctcgcg catgagctcg ccccgccggt
      181 agccgccccc gggggcgcga ttcagcacca cgtggatggg cctgtcctgg tccaccgccc
      241 tgtgccgcac gacccagtcg agcagcctgg ccacaccgac cggggtcggc gcgccgacca
      301 gcacgacctg gtcggcgacc gtgagcagca ggcgggtgat gccgtagcgg gccaggccgt
      361 tcaccgcggt cacgtcctcg gcccggggcc cggcgttgac gatcacctgg cggcagacgc
      421 gggcgagctc cgagaccacg tcggcgacct ggccgggccg gacgttcgtc cagtcccccg
      481 ggctggtcag gccgcagagc acctcgggcc cgtcgccggg cagcggggag agcgactcct
      541 cgaggctgcc cacccgccgc tcgacggcgt cgacggcgtt gcggacgttc gggacgagcg
      601 gcagccccac ccgctgggcc aggcagggcg ccacctcgtc ggcgtcgacc agcacggtcc
      661 gctcgccgcg gcgctcgcag gccacggtca gggcgagcgc gacctcggtg gcgccgcagc
      721 cgccggtcac cgccagcacg accgtgaccc agccgcggcc cgggtcccgc cgctccggcg
      781 gctcgccgcc tggcggcagg tcgtcaccga gctcgcgggt gatccgcgcg aaggcgtcgt
      841 cgacgatggc cgggtcggcc gggccgccgg ccggcgccag gtccgcgagg aggtccacga
      901 tccgctcggg cggggcgttg tcctcgacgg tggcgtcgac gccggtcccc ctgagcgcct
      961 cgggcgcgcg gtagtcgccg gccgcgtgca ccgcgagcac cctgcgaccc cgccggtgca
     1021 gctcgccgac gatgcgcggg gtgaggaagc ccgcggtgtc gtcgacgacc agcaccgcgt
     1081 agtcctcctc cagcgcgtcc tcctcggaga ggacccgcct gcggacgacc gcgccgccgt
     1141 ggtcggccac gtagcggaac aggtcctggg cccaccgccg gctggagacc ccgatggcga
     1201 tctcgaccac ggccatccct agccggtccc cggcgatccg gcggtcgtga gcccggcgcc
     1261 cggaccgggc acgacccggc cggccccggt ggagcggacc acctcgaact tctcgccgcg
     1321 gatggccgcg gccagccgca gggcgtcctg gctcgacacg gccagggtga ggctgtacgc
     1381 gccggccggc ccgatggccg acgggtcggg ccggttgacg ccgagcacct gggcgccggc
     1441 gaccacgaac acggcctgcc cgttgtcgag cacccggacc acgtcgaccc ggtcccccgc
     1501 ctcgagcgcg ccccccaccg cgtgggcggg gtcgaccggc acgctcatcg cgcgctgctg
     1561 ggcggcggcc gccggcggcc ggaagtcccc gcgggtcacg aggtcgccgg gcctgatcgt
     1621 ccgggtggcg acccagccct ggagcccggc gacgccgtcg ggggcgatga gctgctcgcg
     1681 gatggcgcct ggcgcccgca ggtcggtgaa ccggagcgcc gagcgggcga gcggcgcccc
     1741 ggcccggacc tcgcgggcgg cgaccgcgac ccggtaggtg acgtcgccgc cgcgcagcac
     1801 gacgagcacg aggagcatcg cgaggaggcc cgcgagcagc gtgaccagat ggggcaacga
     1861 caagcgggac ccgacgcgcc gcctgggcgg gctcgggctc gacaccttcc gcgcgggcgc
     1921 tgccgcgacg tctgtggcct gcacatcggc ccccgttcac tctgcgatcc acgcttccgc
     1981 agaacggtcg gaccctccgc gaggggtggt ccgacgagga ccggtggcga cgccggaagg
     2041 aacggttcct tcgatggggg aagggtcggc ggcgtcgttt cgttcttggc gtggttgcct
     2101 aattgcagcg gacagtctgc cctacaacca ttcggaagtg aagtcgcgat agtccggggg
     2161 ctacgcacca cccctgctca tagatgcgta gttccccgtg cggcgacgtc gtgtacggtc
     2221 gtgacatggc ggtagaggcg tggctggatc cattcaagga ttccgccgac ctgcccggtc
     2281 ctgccgacct ggctctcggc gcggccgaat cctggcatgc ggccgaaccc tgggacccgg
     2341 atgtcgccgt gcccgagccg gcgacgccgc cgaccggggc cgaccgggcc ccgccgctgc
     2401 tcagccggct cgccgccctg atcgggcccg tccacgaacg ctacaagctc gccgtcctgg
     2461 gcgcgctggc gaggcagcca ggcggcggct ggcggctcgc ggacgcccag gacgccgtcc
     2521 cgtggctcga cccggagtcg acggcacggg tactcggcga gctgtccggg tccggcctgc
     2581 tcgtgccctc gaccgaggcc gggacctggc ggctgagcga cgacgcccag ctgatcgcgg
     2641 ccgtctgctc ggcgctgtcg gtaccccgcg tggagcacga gcgcgtcgtc aaggtgctgg
     2701 ccgcgaccgt cgctctggcg agggccgccg gcgcgcccga cgaggcggcc ttcggcccgt
     2761 tcctggccgc gacgagcgtg ctggagggcg acctcgacgc gatcgagcgg cgcatcgacg
     2821 agggccgtga ggaaccgctg ctcgaggcgg ccgccaccgc ccgcacccac gccgccgaca
     2881 tggcagcgct cctgcagcag cacgccgagc tgttcaccgg cgctggcggg ctggcggacg
     2941 cctgggagcg ggccgtcgcc gtcctcgggc gcctcgagac cctgaccgac gaggtggtgg
     3001 ccgtcctgtc cggccaggcg gacgacaagg tgcgcccggg gccggcggcc gaccggggcg
     3061 ccctgcgccg catcgtcgcc gtggccgacc tggcggcgct ggcctccctg ctgggcgtgt
     3121 cccgcctgcc gccggcgctg acgcccgtcc accccagcgc cgcgctcgcc gcgctcgacg
     3181 agcacctcgg ccggccggac ccgcggcccg tccccatccc cgagcccgtc gccctccccg
     3241 tcgaggagct cgacgacatc cccgacctcg ccacgctcgc cgcccaggcc ctcgagtggc
     3301 tcgccggccg gggcgaggcg gagctggcgg agtgggtggt cggcggctcg tgggccgagg
     3361 cgaccggccg catggcggcg gtcgtcgagg cctggtccag gcacggcccg tgcggcgacg
     3421 ggacgctcgc cgccgagctg gccgcgaggc ctgagctcga cctcgtcggc catgacgagg
     3481 tcggcgccgt ctcgcgcacg ctggtgcgcc ggccggccgg cgggggctcg caccccgagc
     3541 ccgcacgggg ggctcgcacc ccgagcccgg acggggggct cgcgccgtga gcccggtgga
     3601 gctctgccgg gtcctgctcg tcggctcggt gcccgcggcc ggggcccccg agctggccga
     3661 cgacgaggtc cggggcgagg tcaggcggcg gctcgccgag gccg
//
LOCUS       DASPYY010000415         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1615871, whole genome shotgun sequence.
ACCESSION   DASPYY010000415 DASPYY010000000
VERSION     DASPYY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615871"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VEP73_06460"
     CDS             <1..756
                     /locus_tag="VEP73_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYT26106.1"
                     /translation="KAEAFRSVVDGVWSLLPEGAWPAWFLANHDHSRVATRYDVGGTP
                     RRAERDRQKRAERDNDSGQARARVAAMMVLTLRGTPFLYQGEELGMTDAAIPPDRVID
                     VDGRDPERTPMQWDGSPGAGFTTGVPWLPIPEPAREVNAAAERDDPRSMLTLYRRLLR
                     FRRGSAALRLGAYQSLEDAPDGLYAYLRTEGDERLLVALNFTGRPLSYAGRDLPRQGR
                     LELSTDPDRAAGGEVALQPLEVGPDEGVVIRLP"
     gene            complement(823..>1770)
                     /locus_tag="VEP73_06465"
     CDS             complement(823..>1770)
                     /locus_tag="VEP73_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HYT26107.1"
                     /translation="RLRTPTSPAISFGDDPLRMLRAARFEAQLELVPTPDTVAGIRDR
                     LDRLAIVSRERIRDELTKLLLGRAPDKGLWRCVDTGLADQFLPELPALGLEQDPVHRH
                     KDVLRHTIAVVRNVTAFDAQDPDLTLRLAALLHDVGKPRTRRIGPEGVSFHLHEVVGA
                     KMAEKRLTELRYPNEVVHDVRELVALHLRFHTYRLGWTDSAVRRYVRDAGPLLDRLNF
                     LVRSDCTTRNVLKARQLAAYSEELEERIARLEAEEELAKIRPPLDGRQVMEVLGVAPG
                     PVVGRALAHLLELRLDRGPMTEEEATEALLAWASDGGAG"
BASE COUNT          222 a          653 c          671 g          224 t
ORIGIN      
        1 aaggcggagg cgttccggag cgtcgtcgac ggggtctggt cgctcctgcc cgagggcgcc
       61 tggccggcct ggttcctcgc caaccacgac cactcgaggg tcgccacccg gtacgacgtc
      121 ggaggcacgc ctcggcgtgc cgaacgggac aggcagaagc gtgccgaacg ggacaacgac
      181 agcggccagg cccgcgcccg ggtggccgcc atgatggtgc tcaccctgcg cgggaccccg
      241 ttcctctacc agggcgagga gctgggcatg accgacgccg cgatcccgcc cgaccgggtg
      301 atcgacgtcg acggccgcga cccggagcgc acgccgatgc agtgggacgg ctccccggga
      361 gccggcttca ccaccggcgt gccctggctg ccgatccccg agcctgcccg cgaggtcaat
      421 gcggccgccg agcgcgacga cccccggtcg atgctcaccc tgtaccggcg cctgctccgg
      481 ttccgcaggg gctcggccgc gctgcgcctc ggcgcctacc agtcgctcga ggacgcgccg
      541 gacggcctct atgcctacct ccgcaccgag ggcgacgagc ggctgctcgt cgccctcaac
      601 ttcaccggac ggccgctctc gtacgcgggt cgggacctgc ctcggcaggg ccggctcgaa
      661 ctgtccaccg accccgaccg cgcggcgggc ggcgaggtcg cgctccagcc gctcgaggtg
      721 gggcccgacg agggcgtcgt catccggctg ccgtgaccgg cgcagccggc atgcatccgg
      781 cgccgtccga ggcccgggcg ggcagcgccc ccgggcggcg ggtcagccgg cgccgccgtc
      841 cgaggcccag gcgagcagcg cttccgtggc ctcctcctcg gtcatcgggc cccggtcgag
      901 gcgcagctcg aggaggtggg cgagcgccct cccgacgacc gggcccgggg cgacgccgag
      961 cacctccatg acctgccggc cgtccagcgg cgggcggatc ttcgccagct cctcctcggc
     1021 ctcgaggcgg gcgatccgct cctccagctc ctcggagtac gcggccagct ggcgcgcctt
     1081 gaggacgttg cgggtggtgc agtcggaccg gacgaggaag ttcagccggt cgagcagcgg
     1141 cccggcgtcg cggacgtagc gccgcacggc cgagtcggtc caccccaggc ggtaggtgtg
     1201 gaagcggagg tggagggcga ccagctcgcg cacgtcgtgg acgacctcgt tggggtagcg
     1261 cagctcggtc aggcgcttct cggccatctt ggccccgacc acctcgtgga ggtggaacga
     1321 gacgccctcc gggccgatcc ggcgggtgcg gggcttgccg acgtcgtgga gcagcgccgc
     1381 caggcgcagc gtcaggtcgg ggtcctgcgc gtcgaaggcg gtcacgttcc tgaccaccgc
     1441 gatggtgtgg cgcaggacgt ccttgtgccg gtgcaccggg tcctgctcca ggccgagcgc
     1501 gggcagctcg gggaggaact ggtcggccag cccggtgtcc acgcagcgcc agaggccctt
     1561 gtcgggggcg cgcccgagca gcagcttggt cagctcgtcg cggatgcgct cgcgggagac
     1621 gatcgccagg cggtccagcc ggtccctgat ccccgccacc gtgtccggcg tcgggacgag
     1681 ctcgagctgc gcctcgaagc gggccgcccg cagcatgcgc agcgggtcgt cgccgaacga
     1741 gatggccggt gacgtcgggg tgcgcaggcg
//
LOCUS       DASPYY010000416         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1615951, whole genome shotgun sequence.
ACCESSION   DASPYY010000416 DASPYY010000000
VERSION     DASPYY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615951"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1139)
                     /locus_tag="VEP73_06470"
     CDS             complement(204..1139)
                     /locus_tag="VEP73_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26108.1"
                     /translation="MSRTTLAAWLVRLYPSAWRARYADELLAWVAERDLGPLVAVDLA
                     RGALDAHLHPDLAGNRGISMPDRLRGSVLGVLCGYAAFVLAGTGYQKLTEYDDFVAAA
                     HHHAAVGVAFDAVLAGSLVALAAMLAAGAPVGLATVRQALAGHRELRRPLAVVAAGMV
                     WFAGTIALLAWHARTAGPLPAGQMGSAAGFYGWTASLVLAAAVGVAGAVAAVRRAELA
                     AGVLRFAAGAAVVTTCAMVVMLVATVVWGVALRAADPALFHGAEGIRASSTAGSWALI
                     VVCMAAATVVAGVACARGLGAARGGPDPAVVAERS"
     gene            complement(1136..1453)
                     /locus_tag="VEP73_06475"
     CDS             complement(1136..1453)
                     /locus_tag="VEP73_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYT26109.1"
                     /translation="MPSDLSSLGRFAEPSLLILSSLAGGPKHGYAITKDVEELGGPTL
                     GPGTLYAALARLEQLGLIEALPAQERRRPYRLTGVGAEALRAELLRLERLFRVGLQRL
                     ATT"
     gene            complement(1561..>2338)
                     /locus_tag="VEP73_06480"
     CDS             complement(1561..>2338)
                     /locus_tag="VEP73_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26110.1"
                     /translation="QRQPMIELRFFRSASFSGTNLIAFLTFFGLLGFTFFNTLYFQEI
                     RGYSAFRAGLLGLPATLAIAAVAPLAGRLAGARGPGLPIALGTAMAGSAMLLLLGLQV
                     DTAYASIWWIFALSGAGLGLIIAPMTAAAVAGMPQTQAGLASAILNTSRQVGGAVGIA
                     LLGAVFSSHFRAALPPALRPHADGATGSLQVTDPTTRRVIGDAFVTGLHAGYLVAGGA
                     MLVCTLLAVVLLRALHPRQAVPAPPLGPEELEVAAPRSSG"
BASE COUNT          351 a          829 c          923 g          235 t
ORIGIN      
        1 tccaatggtc ccctcggatc gcgttcgggc actgagagcc tagcctaccg gtaccccggg
       61 ccagcctacc ggtacccgaa cccgcggagc gggcggccca gagggtgcgg cgggctcact
      121 ccgcgctgag ggcgaggatc acgtcgaggc gggccgcgcg ccgggcgggc agggtcgacg
      181 cgaccagccc cgcggcggcc gggctacgaa cgctcggcga ccacggccgg gtcggggccg
      241 cctcgggcgg cgccaaggcc ccgggcacac gccaccccgg ccaccacggt ggcggcggcc
      301 atgcacacca cgatcagcgc ccagctgccg gccgtgctgc ttgcgcggat gccctccgcg
      361 ccgtggaaca gggccgggtc ggcggcccgg agcgccacgc cccacaccac ggtggccacc
      421 agcatcacca ccatcgcgca ggtggtgacc acggccgcgc ccgcggcgaa gcggagcacc
      481 cccgcggcaa gctcggcgcg gcgcaccgcc gccacggccc cggccacacc gacggcggcc
      541 gcgagcacga ggctcgcggt ccagccatag aagcccgccg cgctccccat ctggccggcc
      601 gggagggggc cggcggtgcg ggcgtgccag gccagcagcg cgatggtgcc ggcgaaccag
      661 accatccccg cggccaccac cgcgagcggc cgccgcagct cgcggtggcc ggcgagcgcc
      721 tgccgcacgg ttgccaggcc gaccggcgcc ccggcggcca gcatggcggc gagcgcgacc
      781 agcgacccgg ccagcaccgc gtcgaacgcg acgccgaccg cggcgtggtg gtgggccgcc
      841 gccacgaagt cgtcgtactc ggtgagcttc tggtagccgg tgccggcgag cacgaaggcg
      901 gcgtagccgc acagcacgcc gagcaccgag ccgcgcaggc gatcgggcat ggagatcccc
      961 ctgtttccgg cgaggtcggg gtggaggtgg gcgtcgagcg cgccgcgcgc caggtcgacc
     1021 gccaccagtg ggccaaggtc gcgctcggcg acccaggcga gcagctcgtc ggcgtagcgg
     1081 gcccgccagg cgctggggta caggcggacg agccacgcgg ccagggtcgt gcgactcacg
     1141 tggttgccag ccgctgcagg ccgacccgga agagccgctc gagccggagc agctcggcgc
     1201 ggagcgcctc ggcgccgacg ccggtgagcc ggtaggggcg gcggcgctcc tgcgcaggca
     1261 gcgcctcgat caggccgagc tgctcgagtc gggcgagcgc cgcgtacagg gtgccgggcc
     1321 cgagcgtggg cccgcccagt tcctccacgt ccttggtgat ggcgtagccg tgcttgggcc
     1381 cgccggcaag gctcgacaga atcagcagcg acggctccgc gaaccggccg aggctcgaga
     1441 ggtcagaggg catcgcacct cacttctccg atcatcgtta catcgatgat cggagtatag
     1501 gcgcccgtcc cccgcccgtc cagacagctc gtgcggtcat gacgggtacg gccgggcgag
     1561 tcagccgctc gaacgcggcg ccgcgacctc gagctcctcc ggtccgagcg gcggcgcggg
     1621 gacggcctgg cgtgggtgga gggcacggag gaggacgaca gcgagcagcg tgcagacgag
     1681 catggcgcca ccggcgacca ggtagccggc gtggaggccg gtgacgaagg cgtcgccgat
     1741 gacacggcgg gtggtcgggt cggtcacctg gaggctgccg gtggcgccgt cggcgtgggg
     1801 gcgcagggcc ggcgggagcg cggcgcggaa gtggctggag aagaccgcgc cgagcagggc
     1861 gatgccgacc gcaccgccga cctggcggct ggtgttgagg atggccgagg caagccccgc
     1921 ctgtgtctgg ggcatgccgg ccaccgccgc ggcggtcatg ggcgcgatga tgaggccgag
     1981 cccggcgccg gacagcgcga agatccacca gatgctggcg taggcggtgt cgacctggag
     2041 cccgagcagc aacagcatgg cgctgcctgc catggcggtg cccaaggcga tcggcagccc
     2101 cgggccacgg gcgccggcga gccgtccggc gagcggcgcg acggctgcga tcgcgagcgt
     2161 ggcgggcagg ccgagcagac ctgcacggaa cgccgagtag ccgcggatct cctggaagta
     2221 cagggtgttg aagaaggtga agccgagcag gccgaagaac gtcaggaacg cgatcaggtt
     2281 cgtgccggag aacgaggccg agcggaagaa gcgcagctcg atcattggct gccgctgg
//
LOCUS       DASPYY010000417         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1616152, whole genome shotgun sequence.
ACCESSION   DASPYY010000417 DASPYY010000000
VERSION     DASPYY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616152"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEP73_06485"
     CDS             complement(<1..195)
                     /locus_tag="VEP73_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26111.1"
                     /translation="MPRVRLGGTVVVTGRAEAGGPDWVYVPVDVPPGVRRLTVRCAYD
                     RSAGVLDLGLFDANGHGLGAR"
     gene            complement(203..1600)
                     /locus_tag="VEP73_06490"
     CDS             complement(203..1600)
                     /locus_tag="VEP73_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26112.1"
                     /translation="MVPRKLAGEVLTGYLVLAVAGVAVAAALNGHGASALLASALALL
                     VLAVVLAAMLARALAGPLGGLARATSRLVATISPERAGALEAVDPDELATELTVLASR
                     LRDRLDELTEERDRAGQILDALEDGVLLLDGAGRLLVANPVARSWFALPDHYRPGLPA
                     RRVLGVPLVAELAEEAATKQAPVTGTVTVVFPEHRTLSARAFPLADRGPTGRIVVTLT
                     DITQRRRVEVLRRDFVVNASHELKTPVAAVRALSETLQSALSDDPAAARRFATRIARE
                     AERLDLLVRDLLDLSKVERGTLACEPVDLTSLAKEVVGSYADRAEERRIQLRAALEPG
                     ASMRGDRAQLGLLLSNLVDNALHYTSPRGTVRVRLDTCESRVVLQVADTGRGIPASEL
                     PRIFERFYRVDKARDRQSGGTGLGLAIVRHVAESHGGTVQVDSELGRGSTFTVALPVG
                     GPPAPLGPQRSAAAP"
BASE COUNT          221 a          642 c          682 g          194 t
ORIGIN      
        1 cctggcgccg agcccgtggc cgttcgcgtc gaacaggccg aggtcgagca cgccggccga
       61 gcggtcgtag gcgcagcgga cggtcaggcg ccgcacgccg ggcgggacgt cgacggggac
      121 gtagacccag tccggcccgc ccgcctcggc gcgcccggtc accaccaccg tcccgccgag
      181 gcgcacccgg ggcatggtcg ggtcaggggg cggccgccga ccgctgcggg ccgagcggtg
      241 cgggcgggcc gcccaccggc agggcgaccg tgaaggtcga gccccggccg agctcggaat
      301 cgacctgcac ggtgccgccg tgcgactcgg ccacgtggcg gacgatcgcg aggccgaggc
      361 cggtgccgcc gctctggcgg tcgcgggcct tgtcgacccg gtagaagcgc tcgaagatgc
      421 ggggcagctc gctggctggg atgccccggc cggtgtcggc cacctggagc acgaccctgc
      481 tctcgcaggt gtcaaggcgg acccggaccg tcccccgggg gctcgtgtag tggagcgcgt
      541 tgtcgaccag gttggagagc agcaggccga gctgggcccg gtcgccccgc atcgaggcgc
      601 ccggctcgag cgcggcccgg agctggatgc ggcgctcctc ggcccggtcc gcgtacgacc
      661 cgacgacctc cttggcgagc gaggtgaggt cgaccggctc gcaggcgagc gtcccgcgct
      721 cgaccttcga gaggtcgagc aggtcgcgca cgagcaggtc gaggcgctcg gcctcgcggg
      781 cgatgcgggt ggcgaaccgc ctggcggcgg ccgggtcgtc cgacagggcg ctctgcagcg
      841 tctccgacag cgcgcgcacc gccgcgaccg gcgtcttcag ctcgtgggag gcgttgacga
      901 cgaagtcgcg gcgcagcacc tcgacgcgcc ggcgctgggt gatgtcggtg agggtcacca
      961 cgatccgccc ggtcgggccc cggtcggcca gcgggaaggc gcgcgccgaa agggtgcggt
     1021 gctccgggaa caccaccgtg acggtcccgg tcaccggcgc ctgcttcgtg gccgcctcct
     1081 cggccagctc ggcgaccagg ggcacgccca gcacccgccg ggccggcagg ccgggacggt
     1141 agtggtccgg cagggcgaac caggaccggg ccaccgggtt ggccaccagc agcctcccgg
     1201 cgccgtcgag cagcaggacg ccgtcctcga gcgcgtccag gatctggccg gcacggtcgc
     1261 gctcctcggt gagctcgtcg agccggtccc gcaggcgcga cgccaggacc gtcagctcgg
     1321 tggcgagctc gtcggggtcg accgcctcga gggcgcccgc ccgctcgggc gagatcgtcg
     1381 cgaccaggcg gctggtcgct cgcgccagcc cgccgagcgg cccggccagg gcgcgggcga
     1441 gcatggcggc gagcacgacg gccaggacga ggagggcgag ggcgctcgcg aggagcgccg
     1501 atgcgccgtg gccgttcagg gccgcggcga ccgcgacccc ggccaccgcc agcacgagat
     1561 agccggtcag gacctcgccg gcgagcttgc gtggcaccat ggtcagccgc cgtgcccgtt
     1621 tgccgtggcg ctcactcctc gagaccggcg cggaccgcct tcgagggcac gagctcccgc
     1681 acctccccgg tgacgatgaa cacgacctgc tcgccgatgt ccaccgagtg gtcgccgat
//
LOCUS       DASPYY010000418         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2740253, whole genome shotgun sequence.
ACCESSION   DASPYY010000418 DASPYY010000000
VERSION     DASPYY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740253"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEP73_06495"
     CDS             complement(<1..601)
                     /locus_tag="VEP73_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HYT26113.1"
                     /translation="MKGGLLVAGTASDAGKSVVTAGLCRWLARRGIRVAPFKAQNMAL
                     NSAVTPDGAEIGRAQAAQAAAARATPEAAMNPVLLKPTGERRSQVVVMGRPMTDVDAR
                     SYQDLKPRLLEVVLDALADLRRRFDVVVCEGAGSPAEINLRGGDLANMGLARAAGLPV
                     VIVGDIDRGGVFASLFGSLALLDADDQALVAGFLVNKFRG"
     gene            complement(598..1611)
                     /locus_tag="VEP73_06500"
     CDS             complement(598..1611)
                     /locus_tag="VEP73_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HYT26114.1"
                     /translation="MERLLAVTAAGRRRAGWPLAAGVLAGALADRVAGDPARWHPVAG
                     FGALAGALEARCWRPSRLAGAAHVALLVGGSAAAAAAADRRLRTHPVARVAFTAAVTW
                     AALGGRSLGRAATALADAVGAGDLEAGRRLAPSLVGRDPSGLDGPELCRAAVESVAEN
                     TADAVVGPLVWAVLAGPAGVVAYRAANTLDAMVGHQSPRYRRFGWAAARLDDVLTWPA
                     ARLGAAMAAVLAPAVGGDPGGAWRTLRRDGTAHPSPNAGRLEAAFAGALRVRLGGRNR
                     YGERVEDRPPLGDGPPPGPAAVARAARLSALVGGTAAVLAALTALGLGLAREARPAAG
                     VPR"
     gene            complement(1611..2327)
                     /locus_tag="VEP73_06505"
     CDS             complement(1611..2327)
                     /locus_tag="VEP73_06505"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HYT26115.1"
                     /translation="MALVLRRGLLAGLVAGLVSGAYLLVAGEPVIDQALRYEVMPAGH
                     VEVVSRGTQHLGLVVGAVLYALALGGVFAFVAFLLEPRLRGGSAWDRSIRLAAVAFAT
                     VWLVPFLKYPANPPGVGDPTTVGDRSRLYLAMVAVSLGASVAAWQIARRLAERGVEPW
                     RRQLAVGAGYVAVVGLALALLPQVPVRVLVPAQVLWEARLASASGQALLWAVLGAVFG
                     VLSVRAEDRDRPEVPAGAGR"
     gene            complement(2354..2572)
                     /locus_tag="VEP73_06510"
     CDS             complement(2354..2572)
                     /locus_tag="VEP73_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26116.1"
                     /translation="MTARTATTPPTSTIRVPAWAWLVFALLAAFVYAVGYDQGLLLSP
                     VLGKATAANNYLHEFFHDGRHLLGLPCH"
     regulatory      complement(2625..2748)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2992..3807
                     /locus_tag="VEP73_06515"
     CDS             2992..3807
                     /locus_tag="VEP73_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYT26117.1"
                     /translation="MADSSSSLGQRQEPKFDSSVPQTARIWNYWLGGKDNFPADRQVG
                     DQILEAFPEIVENARASRAFLARAVRYLAGEAGIRQFLDIGTGLPTADNTHQVAQAVA
                     PECRIVYVDNDPIVLAHARALLVGAPQGATAYIDADLRDPEKVLQEAARTLDFTQPIA
                     IMLMGILGHIADDGEAQSIVQRLVGAVRSGSYLTMNDGTDISEEVVEAARIWNESANP
                     TYHLRSPDRIARFFDGLELVEPGVVSPPRWRPEPSPSGLPAEIASSCGVGRKP"
     gene            3933..>4570
                     /locus_tag="VEP73_06520"
     CDS             3933..>4570
                     /locus_tag="VEP73_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26118.1"
                     /translation="MATTTSTGRDRARERVQRARAQADQTRRGLRSLLADSAYATVGA
                     GEAAVELVKSIDRIRVEAPKQVRTLGQEAPERLRSLREQGTASVRSLRDQAGQEFDSL
                     AQRGRELVRSISTSPATREAADQARTARSRVKAARTSVTRAAEDTVQAVEEAASTVAS
                     KTEPTAHREVVVGGDRSAATNGGGDEEAVEVRATAARKPRDTRSYEERSVEEL"
BASE COUNT          649 a         1766 c         1680 g          475 t
ORIGIN      
        1 cgccgcggaa cttgttgacg aggaacccgg ccaccagggc ctggtcgtcc gcgtcgagca
       61 gcgcgagcga cccgaacagg ctggcgaaca cgccgccgcg gtcgatgtcg ccgacgatca
      121 cgacgggcag gccggcagcc cgggccaggc ccatgttggc gaggtcgccg ccgcgcaggt
      181 tgatctcggc cgggctgcct gcgccctcgc agacgaccac gtcgaagcgg cgccgcaggt
      241 cggcgagggc gtcgagcacg acctcgagca ggcgcggctt gaggtcctgg taggagcggg
      301 cgtccacgtc ggtcatgggc cgccccatga cgaccacctg gctgcggcgc tcgccggtcg
      361 gcttgagcag caccgggttc atggccgcct cgggcgtcgc ccgggcggcg gccgcctggg
      421 cggcctgggc ccggccgatc tcggctccgt ccggggtgac ggccgagttg agtgccatgt
      481 tctgcgcctt gaacggcgcc acccggatcc cccgccgggc gagccagcgg cacagcccgg
      541 ccgtgaccac gctcttgccc gcgtcggacg cggtgcccgc gacgagcagc cctcccttca
      601 ccggggcacc cccgccgccg ggcgcgcctc gcgggcgagc ccgagcccga gggcggtcag
      661 cgcggcgagc accgcggcgg tcccgccgac gagcgcggag agccgggcgg cacgagccac
      721 cgccgcgggg ccgggcggag ggccgtcgcc gagcggcggc cggtcctcga cccgctcgcc
      781 gtagcggttg cggcccccga gccggacccg cagcgcgccg gcgaaggcgg cctcgagccg
      841 gcccgcgttg gggctggggt gggcggtccc gtcgcggcgg agcgtccgcc acgccccgcc
      901 ggggtcgccg ccgaccgccg gcgcgaggac ggccgccatg gcggccccca ggcgggccgc
      961 gggccaggtg agcacgtcgt cgaggcgggc agcggcccac ccgaaccgcc ggtaccgcgg
     1021 gctctggtgc ccgaccatcg cgtcgagggt gttggcggcc cggtaggcga ccaccccggc
     1081 cgggccggcc agcaccgccc agacgagcgg gccgacgacc gcgtcggcgg tgttctcggc
     1141 caccgactcg accgcggccc ggcacagctc gggcccgtcc aggccgctcg ggtccctgcc
     1201 gaccagggac ggcgcgagcc ggcggccggc ctccaggtcg cccgccccga ccgcgtcggc
     1261 gagggcggtg gccgcccgcc ccagcgagcg gccaccgagg gcggcccagg tcaccgcggc
     1321 cgtgaacgcc acgcgggcga ccgggtgggt gcgcaggcgc cggtcggcgg cggcggccgc
     1381 cgccgccgac ccgccgacga gcagggcgac gtgggccgcg ccggccagcc gcgacgggcg
     1441 ccagcagcgc gcctcgaggg cgccggccag ggcgccgaac ccggccaccg ggtgccaccg
     1501 cgccgggtcg ccggccaccc ggtcggccag ggcgccggcg agcacgccgg ccgcgagcgg
     1561 ccagccggcc cgccgccggc ccgccgccgt cacggccagg aggcgctcca tcacctgccc
     1621 gcgccggccg ggacctccgg ccggtcgcgg tcctcggccc gcaccgacag caccccgaac
     1681 acggcgccca gcacggccca caggagcgcc tggccggagg cggaggccag ccgtgcctcc
     1741 cacaggacct gggcgggcac gaggacgcgg accgggacct ggggcagcag cgcgagcgcg
     1801 agccccacga cggcgacgta gccggcgccg acggcgagct ggcgccgcca cggctcgacc
     1861 ccccgctcgg ccagccgcct ggcgatctgc caggcggcca ccgaggcacc cagcgagacc
     1921 gcgaccatcg cgaggtacag gcggctgcgg tccccgaccg tggtcgggtc gccgaccccg
     1981 ggcgggttcg ccgggtactt gaggaacggg accagccaga cggtcgcgaa cgcgaccgcc
     2041 gccagccgga tcgagcggtc ccacgcggag ccgccccgca ggcgcggctc gagcaggaag
     2101 gcgacgaacg cgaacacgcc gccgagcgcg agcgcgtaca ggacggcacc gacgaccagc
     2161 ccgaggtgct gcgtcccgcg ggacaccacc tcgacgtggc cggcgggcat gacttcgtac
     2221 cggagtgcct ggtcgatgac cggctcaccg gccaccagga gataggcgcc gctcacgagc
     2281 ccggccacca ggccggccag gagcccacgg cgcagcacga gggccacgat gatcctcccg
     2341 cggagccctg gggctagtgg caggggaggc cgagcaggtg ccggccgtcg tggaagaact
     2401 cgtgcaggta gttgttggcg gcggtcgcct tgccgagcac cggcgagagc agcaggccct
     2461 ggtcgtagcc gaccgcgtag acgaacgcgg ccagcagcgc gaagacgagc caggcccagg
     2521 cgggcacgcg gatggtgctg gtgggggggg tggtcgcggt tcgtgcggtc acgtcgagcg
     2581 aacctcctcg gggtcctgcg ccccggtcgg acgacggcga cgaccagtct cctggctccc
     2641 ggatccccgc tccctccggc cttcccaccc gcgagcagtg gccatgctgg aggtcgctcc
     2701 ccggtcacag tggcgcgacc gcgccggact cccaccggct tcctggttcg cgtcgccgtg
     2761 gcgctccgga cgggaccgag gtcccttgag cgccatggcc gcagatactg acgaccatcc
     2821 ggggcggcgt caacccgggg gcgagcctga cccggcgctg cattcagcgg ccgatcgcgg
     2881 ggtgctccgg ctcgctcagc cgtcgttgac atggcggcgt cgctggcgag attctaggct
     2941 ttgtggtgat atcagaggcc gcgcacaacc aaaacatgga gaggcatccc cgtggcagac
     3001 agctcgtcct ccctgggcca gcggcaggag ccgaagttcg acagctcggt gccacagacg
     3061 gcccggatct ggaactactg gctgggcggc aaggacaact tcccggccga ccgccaggtc
     3121 ggcgaccaga ttctcgaggc cttcccggag atcgtcgaga acgctcgcgc ctcccgggcc
     3181 ttcctcgccc gcgcggtccg gtacctggcc ggtgaggcgg gcatccgcca gttcctcgac
     3241 atcggcaccg gcctgcccac cgccgacaac acccaccagg tcgcccaggc ggtggcgccg
     3301 gagtgccgga tcgtctacgt cgacaacgac cccatcgtct tggcgcacgc ccgcgccctg
     3361 ctcgtcggcg ccccgcaggg cgccaccgcc tacatcgacg ccgacctccg cgaccccgag
     3421 aaggtcctgc aggaagcagc ccggacgctg gacttcaccc agccgatcgc gatcatgctg
     3481 atgggaatcc tggggcacat cgccgacgac ggcgaggcgc agtcgatcgt ccaaaggctc
     3541 gtgggcgccg tgcgctccgg cagctacctg acgatgaatg acggcaccga cataagcgag
     3601 gaggtcgtgg aggccgcacg catctggaac gagtccgcca acccgaccta tcacctccgc
     3661 agtcctgatc ggatcgctcg cttcttcgac ggtcttgagc tcgtggagcc cggcgtcgtc
     3721 tcccccccac gatggcgccc cgagcccagc ccgtccggcc tccctgccga gattgcttcg
     3781 tcttgtggcg tcgggcgcaa gccgtagcgc ccggtactcg tcgatctgga actcccggcg
     3841 ccgcgcggga acccagggtt gccaaggtgc ttgcaagtgt ttaacatgtg actaccacag
     3901 taagcgcccc aagacatcga gggagcagca caatggcaac caccaccagc acgggccggg
     3961 atcgtgcccg cgagcgcgtc cagcgcgccc gggcccaggc cgaccagacc cggcgcggcc
     4021 tccggtcgct gctcgcggac tccgcctacg ccaccgtcgg cgccggcgag gcggccgtcg
     4081 agctcgtcaa gtcgatcgac cggatccgcg tcgaggctcc caagcaggtc cggacccttg
     4141 gccaggaggc gcctgagcgc ctccgcagcc tccgcgagca gggcaccgcg agcgtccgca
     4201 gcctccgcga ccaggccggg caggagttcg acagcctcgc gcagcgcggc cgcgagctcg
     4261 tgcggtcgat cagcaccagc cccgccacca gggaggccgc cgaccaggcg cgcaccgccc
     4321 gcagccgcgt gaaggccgcc cgcaccagcg tcacccgggc cgccgaggac acggtccagg
     4381 cggtcgagga ggccgccagc accgtcgcga gcaagaccga gcccaccgcc caccgcgagg
     4441 tcgtcgtcgg cggcgaccgc tcggccgcca cgaacggagg cggggacgag gaggccgtcg
     4501 aggtcagggc gaccgccgcc cgcaagcccc gcgacacccg ttcctacgag gagcggagcg
     4561 tcgaggagct
//
LOCUS       DASPYY010000419         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1899216, whole genome shotgun sequence.
ACCESSION   DASPYY010000419 DASPYY010000000
VERSION     DASPYY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899216"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1192)
                     /locus_tag="VEP73_06525"
     CDS             complement(47..1192)
                     /locus_tag="VEP73_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT26119.1"
                     /translation="MRKLGIIMNGVTGRMGTNQHLVRSVLAIREQGGLPLPDGTVVWP
                     DPVLVGRNEAKLRTLAERHGLERWSTDLAACLASPDDTVYFDAQSTLPRAAALRAAIA
                     AGKHVYCEKPLAVDLATALELARLAADAGVRNGVVADKLFLPGLRKLKRLVDGGFFGR
                     ILSLRGEFGYWVFEGDWQAAQRPSWNYRAEDGGGILADMFCHWRYLLDHTFGPVQAVS
                     CHAATHVPERVGEDGRPYRATAEDAAYGTFLLGGGIVAQINSSWCVRVYRDELFSLQV
                     DGTGGSAVAGLRQCRAQHRAATPKPEWNPDVPNPIDFRAGWQEVPDNAAWDNGFKAQW
                     ELFLRHVLAGEPFPWDFLEGARGVQLAELGARSWAERRWVDVPPIAV"
     gene            complement(1299..>1575)
                     /locus_tag="VEP73_06530"
     CDS             complement(1299..>1575)
                     /locus_tag="VEP73_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYT26120.1"
                     /translation="DAVVAANDESAIGVLMALRQAGVDVPGQVSVAGMGDTRASRFVD
                     LTTVSVPMYELGALAAERILEAGETPPPAETILAHRLVARSTTRRRT"
BASE COUNT          205 a          561 c          605 g          204 t
ORIGIN      
        1 cgatcggggc gggggagcag gacgctcggc ggcatctggc tgcggcctag acggcgatcg
       61 gcgggacgtc gacccagcga cgctcggccc aggaccgggc gccgagctcg gcgagctgga
      121 cccctctggc gccctcgagg aagtcccacg ggaacggctc gccggccagc acgtggcgca
      181 ggaacagctc ccactgggcc ttgaagccgt tgtcccaggc tgcgttgtcg ggcacctcct
      241 gccagccggc ccggaagtcg atcgggttcg gcacgtcggg gttccactcc ggcttcgggg
      301 tcgccgcccg gtgctgggcg cggcactgcc gcaggcccgc cacggcgctg cccccggtgc
      361 cgtcgacctg caggctgaac agctcgtcgc ggtagacgcg gacgcaccac gacgagttga
      421 tctgggcgac gatccccccg ccgagcagga aggtcccgta ggcggcgtcc tcggccgtgg
      481 cccggtaggg ccgcccgtcc tcgccgaccc gctcggggac gtgggtggcc gcgtggcagg
      541 agaccgcctg gaccggcccg aaggtgtggt cgaggaggta gcgccagtgg cagaacatgt
      601 cggccaggat gccgccgccg tcctcggccc ggtagttcca ggacggccgc tgcgccgcct
      661 gccagtcgcc ctcgaacacc cagtacccga actcgccgcg cagcgacagg atgcggccga
      721 agaacccgcc gtcgaccagc cgcttgagct tgcgcaggcc gggcaggaac agcttgtcgg
      781 ccaccacgcc gttgcgcacg ccggcgtcgg cggccaggcg ggcgagctcg agggcggtgg
      841 ccaggtcgac cgcgagcggc ttctcgcagt agacgtgctt gcccgcggcg atcgcggcgc
      901 gcagcgcggc ggcccgtggc agggtcgact gggcgtcgaa gtagacggtg tcgtccgggc
      961 tggccaggca cgcggcgagg tcggtgctcc agcgctcgag cccgtggcgc tccgcgaggg
     1021 tgcgcagctt ggcctcgttc cgcccgacca ggacggggtc gggccagacg acggtgccgt
     1081 ccggcagcgg caggccgccc tgctcgcgga tggccaggac cgaccgcacg aggtgctggt
     1141 tggtgcccat gcgcccggtg accccgttca tgatgatgcc gagcttccgc atggtcgcgc
     1201 tcgcttctcg agccgggatc cgcgccggga ggatggcaaa cgatttccat gaggaccgaa
     1261 cgggcggggc cggccactgt caagcatcgg gccggcggtc aggtccggcg gcgggtggtg
     1321 ctccgcgcga ccagccggtg ggcgaggatg gtctcggcgg gcggcggggt ctcgccggcc
     1381 tccaggatgc gctcggcggc gagcgcgccc agctcgtaca tgggcacgct gaccgtggtc
     1441 aggtcgacga accgggaggc gcgggtgtcc cccatgccgg ccaccgagac ctgcccgggg
     1501 acgtccaccc cggcctgccg tagggccatg agcacgccga tggcgctctc gtcgttggcc
     1561 gccaccaccg cgtcc
//
LOCUS       DASPYY010000420         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1756921, whole genome shotgun sequence.
ACCESSION   DASPYY010000420 DASPYY010000000
VERSION     DASPYY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756921"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2853
                     /locus_tag="VEP73_06535"
     CDS             <1..>2853
                     /locus_tag="VEP73_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HYT26121.1"
                     /translation="EPDRVPPADEFLVLDADASQNLAVDAAVRGEDLVLDGPPGTGKS
                     QTIANLVATLVARGQRVLFVAEKRAAIDAVLGRLAARGLADLVLDLHGGGTTRRKVAS
                     DLARALTEAGGVPRVDLADVHRTVQLRRDELSSHADAVHRRRAPWQVSVYGMQARLVG
                     ADPAVRTPVRFRGTELARLDAATYRQVAEDLRAYAGLGGLGLASGDSPWAGATVTSPE
                     EAEAALDTVATLAHHTLPAATAALDRALAETGLARPSTLGEWRQTVALLDGVSAVATL
                     FSEDVWAQDLDGLATAFAPAGRGPLARLAAWLASARYRHAAGTLRGLCRERPPKPTAL
                     RGACVYAGEQRRRWEELTVDGGPPRPPGDLPGVVSTYNQLGAELRSLAAWLPPREVDR
                     RPVTELGALLDRLLADQATLYKLPELHRLEQELNRAGLRYPLAELRRRQLGVDQCLAM
                     FEHAWLSSICEHVAATDPRIGAFDGSLHARTVADYRAADAIHVETTATRVRRACAERA
                     TRVRDDYPEESTLVEHQARLARGHLSLRQLVQRAPHILTALKPCWAMSPLVVSQLLPG
                     DRPYFDVVVFDEASQIRLADAVPAILRGARVVVAGDERQLPPMSFFASTTVDDEDDET
                     DDPGLGAALGMTQGFESVLGALGALLRSRTLTRHYRSRDERLIAFSNAHVYDRALVTF
                     PGAAGGDCIDHVLVPFRPGQAGPEESVTDEVHEVVRLVLEHASARPEESLGVITMGVR
                     HANRVTKALRLARAERPELADFFVEGRGERFFVKDFERVQGDERDAIILSVGYGKNQR
                     GELPYRFGPLLQQDGERRLNVAVTRARRRVTLVSSFSSADMDPARSASKGVELLRLFL
                     RYAERRGADLDAEAAAAPAPDPFEIDVRDRLEAAGIPLAAKHGCSGYRIDFAARHPTR
                     PGRFVLAIECDGASYHASPTARDRDRLRQDHLERLGW"
BASE COUNT          350 a         1204 c          964 g          335 t
ORIGIN      
        1 gagcccgacc gtgtcccgcc cgccgacgag ttcctggtgc tcgacgccga cgcgagccag
       61 aacctcgcgg tcgacgccgc cgtccgcggc gaggacctgg tcctcgacgg cccgccgggg
      121 acgggcaaga gccagaccat cgccaacctc gtcgccaccc tggtcgcccg cggccagcgg
      181 gtgctgttcg tcgccgagaa gcgcgccgcc atcgacgccg tgctcggccg cctcgccgcc
      241 cgcggtctcg ccgacctcgt cctcgacctg cacggcggcg ggaccacccg ccgcaaggtc
      301 gcaagcgacc tggccagggc gctgaccgag gccggcggcg tcccgcgcgt cgacctggcc
      361 gacgtccacc ggaccgtcca gctccgccgc gacgagctga gctcccacgc cgacgccgtc
      421 caccggcgcc gcgcgccctg gcaggtcagc gtctacggca tgcaggcgcg cctcgtcggc
      481 gccgaccccg ccgtccgaac cccggtccgc ttccgcggca ccgagctggc ccgcctcgac
      541 gccgccacct accggcaggt ggccgaggac ctgcgcgcct acgccggcct cggcggtctc
      601 ggcctcgcgt ccggcgacag cccgtgggcc ggcgccaccg tcacgagccc cgaggaggcc
      661 gaggctgcgc tcgacacggt cgcgaccctc gcgcaccaca cgctcccggc cgcgaccgcg
      721 gccctcgacc gcgcgctggc cgagacgggc ctcgcgcgac cgtcgaccct gggcgagtgg
      781 cggcagaccg tcgccctcct cgacggggtg tcggccgtcg ccaccctctt cagcgaggac
      841 gtctgggccc aggacctgga cgggctcgcc accgcgttcg cccctgccgg ccgcggcccc
      901 ctcgcccggc tcgccgcctg gctggcctcc gcccgctacc gccacgcggc cgggacgctg
      961 cggggcctgt gccgcgagcg gccgcccaag cccaccgccc tgcgcggcgc ctgcgtctac
     1021 gccggcgagc agcggcgccg ctgggaggag ctgaccgtgg acggcgggcc gccccgcccg
     1081 cccggcgacc tgcccggcgt ggtcagcacc tacaaccagc tcggcgccga gctccgctcc
     1141 ctggcggcat ggctgccccc gcgggaggtc gaccggcggc cggtgaccga gctgggcgcg
     1201 ctgctcgacc gcctgctcgc cgaccaggcc accctctaca agctgcccga gctccaccgc
     1261 ctcgagcagg agctcaaccg cgccggcctg cgataccccc tcgccgagct ccgccggcgc
     1321 cagctcggcg tcgaccagtg cctcgcgatg ttcgagcacg cctggctctc ctcgatctgc
     1381 gagcacgtcg cggccaccga cccacgcatc ggcgccttcg acggctcgct gcacgcccgc
     1441 acggtcgccg actaccgggc cgccgacgcc atccacgtgg agaccaccgc gacccgggtc
     1501 cggcgcgcct gcgcggagcg cgccacccgg gtccgcgacg actacccgga ggagagcacc
     1561 ctcgtcgagc accaggcccg gctcgcccgc gggcacctgt cgctcaggca gctcgtgcag
     1621 cgcgcccccc acatcctcac cgccctgaag ccctgctggg cgatgagccc cctggtcgtc
     1681 agccagctcc tcccgggtga ccggccgtac ttcgacgtcg tcgtcttcga cgaggccagc
     1741 cagatccgcc tcgccgacgc cgtcccggcg atcctgcgcg gcgccagggt ggtcgtggcc
     1801 ggcgacgagc ggcagctccc gcccatgtcc ttcttcgcgt cgacgaccgt cgacgacgag
     1861 gacgacgaga ccgacgaccc cggcctcggc gccgccctgg gcatgaccca ggggttcgag
     1921 tccgtcctcg gcgcgctcgg cgcgctgctc cgctcgcgga cgctcacccg gcactaccgc
     1981 agccgggacg agcggctcat cgccttctcc aacgcccacg tgtacgaccg ggcgctggtc
     2041 acgttccccg gcgcggccgg cggcgactgc atcgaccacg tcctcgtccc cttccggccc
     2101 ggccaggccg gcccggagga gagcgtcacc gacgaggtcc acgaggtcgt gcgcctggtg
     2161 ctggagcacg ccagcgcccg ccccgaggag agcctcggcg tcatcaccat gggcgtcagg
     2221 cacgccaacc gcgtcaccaa agcgctcagg ctggccaggg ccgagcgccc ggagctcgcc
     2281 gacttcttcg tcgagggccg cggcgagcgg ttcttcgtga aggacttcga gcgcgtccag
     2341 ggtgacgagc gcgacgcgat catcctctcc gtcggctacg gcaagaacca gcgaggcgag
     2401 ctgccctacc gcttcggacc gctgctgcag caggacggcg agcgccgcct caacgtcgcc
     2461 gtcaccaggg ccaggcggcg cgtgacgctc gtgtcgtcgt tctcgtcggc cgacatggac
     2521 cccgcccgca gcgcctccaa gggcgtggag ctgctgcggc tgttcctccg gtacgccgag
     2581 cgccggggcg ccgacctcga cgccgaggcc gccgccgcgc cggcgcccga cccgttcgag
     2641 atcgacgtcc gcgaccggct ggaggccgcc gggataccgc tcgccgccaa gcacggctgc
     2701 tccgggtacc gcatcgactt cgccgccagg cacccgaccc gtcccggccg gttcgtcctg
     2761 gcgatcgagt gcgacggcgc cagctaccat gcgtccccga ccgcccgcga ccgcgaccgg
     2821 ctgcggcagg accacctgga gcggctcggc tgg
//
LOCUS       DASPYY010000421         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_634204, whole genome shotgun sequence.
ACCESSION   DASPYY010000421 DASPYY010000000
VERSION     DASPYY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634204"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VEP73_06540"
     CDS             complement(<1..1706)
                     /locus_tag="VEP73_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT26122.1"
                     /translation="MAVSRPRVLAVVVVHDGRPFLRDTFRGLAAQTRPIDDVVIVDTG
                     STDGSADWARARLGADAVLAVRGQFGRAVMSALRDERAAGMDWLWLLHDDSAPEPEAL
                     ERLLAEAESRPSASILGPKLVSWNDPRRLAEVGWSVDRTGRAISPVEDDEIDQGQHDQ
                     IRDVFFVSTAGMLVRRGTLSAVGGFDERISAFRDDLDLCWRTHLVGGRVLVVPAARVR
                     HFGAAASRSRRTRAVSLPRYLAERHTTMVMLKATRLRRLPLVGLLAFLGALVRIAGLT
                     LTGRPGDAAQVLWAWGWNVKELPITLVHRRRIQARRKVDDRMLASLHAPGGQRVRSLL
                     RGWLDLLYGDAGEHVARPTFKEEEDTPNVGASVGRVLARHPVGFMVAAFAVLMAVSLR
                     SVLIAPGLAGGSLALWPSTAQSLLAEYRSVFHPGGLGSSEPASPAIALLGVLSTLTFG
                     KALLAEKLLLWLALPFAAAACTRALRVLVAELPARAVAGLLYASSPLAIGAIAQGRTG
                     ELVFLIFAPLALAQVVLAFRPTQPREPWRPALRFALLAAVSVAMYPPALVVLGLVVLL
                     AVG"
     gene            complement(2148..2408)
                     /locus_tag="VEP73_06545"
     CDS             complement(2148..2408)
                     /locus_tag="VEP73_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYT26123.1"
                     /translation="MDQEASVSPLLPVEIQPWYERAACLDKNADCFFPEKGGSTREAK
                     RICSTCSVVADCLRYALANDERFGIWGGMSERERRRLKRLAS"
     gene            2695..3333
                     /locus_tag="VEP73_06550"
     CDS             2695..3333
                     /locus_tag="VEP73_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26124.1"
                     /translation="MDDGELTRADLVAARRRDPAAVTRIYRAYAPALYRFFVAALGDR
                     HAAEDMTGTVFAAAIEALPGFRGPVEALGGWLFRIARHDLYDHRRKEAKTRTESLDDV
                     LDEAAMAVGAPDPQEIVVERLEGSRLMAALDELSVDQREVLLLRIAAGLTAPEVAATL
                     GKTTGAVKALQHRGLASLARLLGLRGDGRTDELAEDPYPSEPTERLPRQEEG"
     gene            3330..4517
                     /locus_tag="VEP73_06555"
     CDS             3330..4517
                     /locus_tag="VEP73_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26125.1"
                     /translation="MTMGRSWDLDEELDHELDALLDAPGGGLPDERHELAPLVEVAAA
                     LRAELAAVELDPGLAHQHVGRAVGAPRLRPAVVPVRSLHGSNSVHGSAVRMGTEVPAA
                     PAAPPVGRPARFTLRRRVAVLALAAALLLVPATMASTRALPGQPLYPLKRSVEQVRLA
                     ALGWSPAASARERLRIADVRSSELASLLARGDVGRVPGAIVALRSAVDAAWQAVDEAS
                     FRDVDSSRAVALRQDFAAVQDRQIIQLTRVSGSIQTSSPAAEQAIETATSALASAKKQ
                     KQKQQQQQQQGARGGGRPAPTSPTTPPTSPTVGALLPDLATTVTAPTAGAPTPTVATA
                     TTPTATATTAAVAARAAAALLDCGDRPKKAKEREDWAECMAGYGQPVDPADVAAAEDG
                     DAG"
     gene            4656..5588
                     /gene="cofD"
                     /locus_tag="VEP73_06560"
     CDS             4656..5588
                     /gene="cofD"
                     /locus_tag="VEP73_06560"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HYT26126.1"
                     /translation="MLVALAGGVGAARFLRGVVRTHHPSDVTVIGNTGDDLRMHGLAI
                     SPDLDSVMYTLAGLADEERGWGLVDETWNVRDALVRLGEPGWFGLGDADIATHLLRTR
                     LLAEGASLSQATLELCRRLGVPARLVPMSDDRVETRVEVDGPDGPEDLGFQEYWVARH
                     AADPVRRIRFRGVEHARPAPGVLDAIAAAEGIVVCPSNPVVSVAPILAVPGILEAVQA
                     APCRKVAISPIVGGVPVRGMADRLLPAWGVEVSARGVAGLYADLVDAFVLDEVDAGQA
                     ADVAALGLRAVVAPTVMHTPDDAAALAKTALSLL"
     gene            5636..>6029
                     /locus_tag="VEP73_06565"
     CDS             5636..>6029
                     /locus_tag="VEP73_06565"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYT26127.1"
                     /translation="MPLPGLPEVGPGDDLARLLLDGLAAAGERLADGDVVAVTSKVVA
                     KSEGRVVPLPADPAERERVHRETVAAETVRVVARRDRMVIAETRHGLVGANALVDASN
                     TGADALVLLPADPDASAARLRAALAAASG"
BASE COUNT          787 a         2256 c         2285 g          701 t
ORIGIN      
        1 ccgacggcca ggagcaccac gagcccgagg acgacgagcg ccggcgggta catcgcgacg
       61 gagaccgcgg cgagcagcgc gaaccggagg gcgggacgcc acggctcgcg cggctgggtc
      121 ggccggaacg cgagcacgac ctgggcgagc gcgagcgggg cgaagatcag gaagacgagc
      181 tcgccggtgc ggccctgggc gatggcgccg atcgcgagcg gcgagctcgc gtacagcagg
      241 ccggccaccg cccgcgcggg cagctcggcg accagcaccc gcagggcccg cgtgcaggcc
      301 gcggcggcga acggcagcgc cagccacagc aggagcttct cggcgagcag cgccttgccg
      361 aaggtcaggg tcgaaaggac gccgagcagg gcgatggcgg gcgaggcggg ctcgctcgac
      421 ccgagcccgc cggggtggaa caccgaccgg tactcggcca ggagcgactg ggcggtcgac
      481 ggccataggg cgagggagcc gccggcgagg ccgggggcga tcagcaccga gcgcagcgac
      541 acggccatga gcaccgcgaa cgcggcgacc atgaagccga ccgggtggcg cgcgagcacc
      601 cggcccaccg aggcgccgac gttgggcgtg tcctcctctt ccttgaaggt gggccgggcg
      661 acgtgctcgc cggcgtcgcc gtagaggagg tcgagccagc cgcgcagcag cgagcgcacg
      721 cgctggccgc ccggtgcgtg gaggctcgcg agcatgcggt cgtcgacctt gcggcgggct
      781 tggatacgcc gccggtgaac gagcgtgatc ggcagctcct tgacgttcca cccccacgcc
      841 cagagcacct gggcggcgtc gccggggcgg ccggtgaggg tcaggcccgc gatccggacg
      901 agcgcgccga ggaaggcgag cagccccacc agcggcagcc ggcggaggcg ggtcgccttg
      961 agcatgacca tcgtggtgtg ccgctcggcc aggtagcggg gcagcgacac tgcccgggtg
     1021 cggcggctgc gggaggccgc cgcgccgaaa tggcggaccc tggcggccgg caccacgagc
     1081 acccgcccgc cgacgaggtg ggtgcgccag cacaggtcga ggtcgtcgcg gaacgccgag
     1141 atgcgctcgt cgaagccgcc gacggccgag agcgtgccgc gccgcaccag catccccgcg
     1201 gtggagacga agaacacgtc gcggatctgg tcgtgctggc cctggtcgat ctcgtcgtcc
     1261 tcgaccgggg agatcgcccg gccggtccgg tcgaccgacc agccgacctc ggcgaggcgc
     1321 cgcgggtcgt tccaggagac gagcttgggg ccgaggatgc tggcgctcgg cctggactcg
     1381 gcctcggcga ggagccgctc gagcgcttcc ggctcggggg cggagtcgtc gtggagcagc
     1441 cacagccagt ccatgccggc ggcgcgctcg tcgcggagcg cgctcatgac ggcgcggccg
     1501 aactggccgc ggacggcgag gacggcgtcg gcgccgaggc gggcgcgcgc ccagtcggcg
     1561 ctgccgtccg tcgacccggt gtcgacgatc acgacgtcgt cgatcgggcg ggtctgggcg
     1621 gccagcccgc ggaaggtgtc ccgcaggaat ggccgcccgt cgtgcacgac gaccactgcc
     1681 aggacccttg gtcgactcac tgccactgcc aggctcctcg acgcggaggt tcagccgcgt
     1741 attttgccac catgcgaaac gcccgcgggt cacgctgact cccacgggcg cggcggcatc
     1801 ctagcaccgc cggacgccgg tccgggcgtg ctgcgacgcc cacgccggcc ggtccacgcc
     1861 cgctccccgg gcaggccgcc ggcccgggca cccggcatgg cgcgggggcg ccgattcggc
     1921 gccggcgccc gtttcgacgc cgcgaccggc gcggttgcgc cacggcgtcg cgaccgcacg
     1981 cgggtcggcg gcggccggga gcccggccgg gcgcgccgcc ggccgctgcc ggggcggggg
     2041 cgcgcggcgt gccccggcgg ggggccgtcg tgggcggctc gcgtccccgg tcgcccggcg
     2101 cgcgctcaag atccccagaa gtgcctagga gacaagctca gaagtgccta cgaggcgagg
     2161 cgcttgaggc ggcggcgctc gcgctcgctc atgccgcccc agatcccgaa ccgctcgtcg
     2221 ttggcgagcg cgtaccgcag gcagtcggcg accacgctgc aggtcgagca gatgcgcttg
     2281 gcctcgcggg tcgacccgcc cttttcggga aagaagcagt ccgcgttctt gtcgaggcag
     2341 gcggcgcgct cgtaccaggg ctggatctca actggaagga gtgggctcac gctagcctcc
     2401 tgatcgatgc acggcggcca attcgcgggc gcggacgccc gtcacggaac ggcaaggccg
     2461 taattacatc ggtggcattc tcgagcacct tgagggggaa aatcaagaag tttggtattt
     2521 cttcgccacc aaaccgtgac ctcgctcgcg gcgcccgggc tcccctgtcc ggggccgatc
     2581 agatagcctc taggccgccg gacagcggga agccgagggg cgttcgtgtt cacgaccaga
     2641 ccggcagcgg agtcgcaggg tggggagggc gcggagggcg cctcccggca ggaggtggac
     2701 gacggcgagc tcacgcgtgc cgacctggtc gccgcgcggc gccgggaccc cgccgccgtc
     2761 acgcgcatct accgcgcgta cgcgcccgcg ctgtaccgct tcttcgtcgc cgcgctcggc
     2821 gaccggcacg ccgccgagga catgaccggc accgtcttcg ccgccgccat cgaggcgctg
     2881 cccggcttcc gcggcccggt cgaggccctc ggcgggtggc tgttccgcat cgcccgccac
     2941 gacctctacg accaccggcg caaggaggcg aagaccagga ccgagagcct cgacgacgtc
     3001 ctcgacgagg cggccatggc ggtcggtgct cccgacccgc aggagatcgt cgtcgagcgc
     3061 ctcgagggca gccggctgat ggccgcgctc gacgagctgt cggtcgacca gcgcgaggtg
     3121 ctgctgctgc gcatcgccgc cggcctgacc gcgcccgagg tcgcggcgac cctcggcaag
     3181 accaccggcg ccgtcaaggc cctgcagcac cgcggcctcg ccagcctcgc caggctgctc
     3241 ggcctgcgcg gtgacggtag aactgacgag ctcgccgagg acccgtatcc atcggaaccg
     3301 accgaacgcc ttcctaggca ggaggagggg tgacgatggg ccggtcctgg gatctcgatg
     3361 aggagctcga ccacgagctc gacgcactgc tcgacgcgcc cggaggcggg ttgcccgacg
     3421 agcggcacga gctcgcgccg ctcgtcgagg tcgccgccgc gctgcgcgcc gagctggccg
     3481 cggtcgagct cgaccctggg ctcgcccacc agcacgtggg ccgcgcggtc ggcgcgccca
     3541 ggctccggcc cgcggtggtc ccggtgcgct cgctgcacgg gtcgaacagc gtgcacgggt
     3601 cggccgtgcg gatgggcaca gaggtcccgg ccgcaccggc cgccccgccg gtcgggcgcc
     3661 cggcccgctt cacgctccgg cgccgtgtcg ccgtgctcgc cctcgcggcc gcgctgctcc
     3721 tggtccccgc gacgatggcg tcgacccgcg cactgcccgg ccagccgttg tacccgctga
     3781 agcgctcggt cgagcaggtg cgcctcgccg cgctgggctg gtcgccggct gccagtgcgc
     3841 gggagcgcct gcgcatcgcc gacgtccgct cgagcgagct cgccagcctg ctcgccaggg
     3901 gagacgtcgg ccgggtgccc ggcgcgatcg tggcgctccg cagcgcggtc gacgccgcct
     3961 ggcaggcggt ggacgaggcg tcgttccggg acgtggactc gagccgggcg gtcgcgctgc
     4021 ggcaggactt cgccgccgtg caggaccgcc agatcatcca gctcaccagg gtcagcggct
     4081 cgatccagac ttcctcgcca gcggctgagc aggcgatcga gaccgccacc agcgcgctcg
     4141 cgagcgcgaa gaagcagaag cagaagcagc agcagcagca gcagcaaggg gcgcggggcg
     4201 gcggcaggcc agcaccgacc tccccgacca cgccgccgac gagcccgacc gtcggcgcgc
     4261 tgctcccgga cctcgccacg accgtcaccg cgcccacggc cggcgcgccg accccgacgg
     4321 tggcgaccgc caccacgccg acggcgaccg ccaccacggc ggcggtcgcc gcgcgggccg
     4381 cggccgccct gctcgactgc ggggaccggc ccaagaaggc gaaggagcgc gaggactggg
     4441 ccgagtgcat ggccgggtac ggccagccgg tcgaccccgc cgacgtcgcc gcggccgaag
     4501 acggcgacgc cgggtagcga cgcggcgcgg gaccgtccgt cccgcgcgta tccttgcgtc
     4561 ccgtccggtt ctctcagtcg tccggttccc tcagccgtcc ggtccctcag ccgtccggtc
     4621 cctcagtggt ccggtccctc agtgaaggag cgcccatgct cgtcgcgctc gcgggcggtg
     4681 tcggtgccgc aaggttcctg cgcggcgtgg tccggaccca ccacccgtcc gacgtcaccg
     4741 tcatcggcaa caccggcgac gacctgcgga tgcacgggct cgccatctcc cccgacctcg
     4801 actcggtcat gtacacgctg gccgggctgg ccgacgagga gcgcggctgg gggctcgtgg
     4861 acgagacctg gaacgtccgc gacgccctcg tccggctggg cgagccgggc tggttcgggc
     4921 tcggtgacgc cgacatcgcc acccacctgc tccgcacccg cctgctcgcc gagggcgcgt
     4981 cgctcagcca ggccacgctc gagctgtgcc gtcggctcgg ggtacccgcc cgcctcgtgc
     5041 ccatgagcga cgaccgggtc gagacccgcg tcgaggtcga cggcccggac ggtcccgagg
     5101 acctcgggtt ccaggagtac tgggtcgcca ggcacgccgc cgacccggtg cgccggatcc
     5161 ggttccgggg cgtggagcac gcgaggccgg cgcctggcgt gctcgacgcg atcgccgccg
     5221 cggagggtat cgtcgtctgc ccgtccaacc cggttgtctc ggtcgccccg atcctggccg
     5281 tgcccggcat cctcgaggcg gtccaggccg ccccgtgccg gaaggtcgcg atcagcccga
     5341 tcgtcggcgg ggtcccggtc cgtggcatgg ccgacaggct gctgccggcc tggggcgtgg
     5401 aggtgtccgc gcgcggcgtg gccggcctct acgccgacct ggtcgacgcg ttcgtgctgg
     5461 acgaggtcga cgccgggcag gccgcggacg tggccgccct cggcctgcga gccgtcgtcg
     5521 ccccgaccgt catgcacacc cccgacgacg ccgccgcgct cgcgaagacc gccctgagcc
     5581 tgctgtgacc ccgcgcggga ctcccccttc cccagcagcg cgggcgctca ccgtcgtgcc
     5641 cctgcccggg ctcccggagg tcgggccggg cgacgacctc gcccggctcc tgctcgacgg
     5701 gctggccgcc gccggtgagc ggctcgccga cggcgacgtc gtcgccgtga cgtcgaaggt
     5761 ggtcgccaag tccgaggggc gggtcgtccc gctgcccgcc gacccggccg agcgggagcg
     5821 ggtccaccgg gagacggtgg cggccgagac ggtccgggtg gtggccaggc gggaccgcat
     5881 ggtgatcgcc gagaccaggc atgggctcgt gggcgccaac gcgctggtcg acgcgtccaa
     5941 caccggcgcc gacgcgctgg tcctcctgcc cgccgacccg gacgcctcgg cggcccgtct
     6001 gcgggccgcg ctcgccgccg ccagcggcg
//
LOCUS       DASPYY010000422         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1899434, whole genome shotgun sequence.
ACCESSION   DASPYY010000422 DASPYY010000000
VERSION     DASPYY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899434"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1968
                     /locus_tag="VEP73_06570"
     CDS             <1..>1968
                     /locus_tag="VEP73_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26128.1"
                     /translation="PWLLALFGAGALWLVQVGFQRANAASVTAALTALDSLGPILAGF
                     LLYHERWPAGGDRVLLTAGAATAVAGLTVLSARREAAEPAPDLPSGEPPPAARGLRAP
                     VRLSIMRGAERGAGGAGKVAAATGRPPGNEDSEEIPGVPNGRDDLVSDSGMDVADRTF
                     EAVVLYWDGLAGDAEVAAALRGRVAALRSAGAEVAIVSRTPVEGFDGQLLAGPEPAGR
                     LRRYERGEGEHAADTTRRVLADLRTHGIGPELVLAAGAPDGEARALAEARVTVVPVRP
                     ALGPGSDPSALVGLLDEQLARRAARRVQGVDADPGWTVAVDGGDTRPRQVAETLLTVA
                     DGLLGTRGVREEDGAGPAPATVAAGVYDDRSETVPLLPGPGWTGLEVAAGAGRERRVL
                     DLRSGVLWRERADDGAPLRTLRFSSLARPGVVALRAEGGPAVLRPGPALLPPGGEAGF
                     EQGRLGDVTWARTRARRDGGITAAARQREGGGDRATVERIAAYVADPTGAPGPEEAAE
                     RLRAAEAVGFDQLLAEHRRAWAARWADAEVTIEGDPRSEQAVRFALFHLLANVADRGE
                     AAVGARGLSGPAYAGHVFWDADVFVLPTLAATHPAAARAMLEYRVRRLPAARRIAADL
                     GYAGARFPWESAADGNEVTPATTVDERGEVIE"
BASE COUNT          195 a          758 c          803 g          212 t
ORIGIN      
        1 ccctggctgc tcgcgctgtt cggcgccggt gcgctgtggc tcgtccaggt gggcttccag
       61 cgcgccaacg ccgcctcggt gaccgcggcg ctgacggcgc tggactcgct cggcccgatc
      121 ctcgccggct tcctgctgta ccacgagcgc tggccagcgg gcggcgaccg ggtcctgctc
      181 accgccggcg ccgccacggc ggtcgcgggg ctcacggtgc tctccgcgcg ccgcgaggcc
      241 gccgagcccg cgccggacct gccgagcggc gagccgcccc cggcggcccg cgggttgcgg
      301 gcacctgtac ggttgtccat catgcgcggc gcggagcgcg gcgcgggggg cgccgggaag
      361 gtggccgcgg ccaccggccg tccgcccggg aacgaggact cggaggaaat acctggcgtg
      421 ccgaacggga gagatgacct ggtgagcgac agcggcatgg acgtggcgga ccgcaccttc
      481 gaggcggtcg tcctctactg ggacggcctg gccggcgacg ccgaggtcgc tgccgcgctc
      541 cgcggccggg tcgcggccct gcggtcggca ggcgccgagg tggcgatcgt gagccggacg
      601 cccgtcgagg ggttcgacgg gcagctcctg gccggcccgg agcccgccgg ccggctgcgg
      661 cgctacgagc ggggcgaggg ggagcatgcc gcggacacca cgcggcgggt gctcgccgac
      721 ctccggacgc acgggatcgg cccggagctc gtgctcgccg ccggcgcccc ggacggcgag
      781 gcccgcgcgc tcgccgaggc ccgcgtgacg gtggtgccgg tgcggccggc cctcgggccc
      841 ggcagcgacc cgtccgccct ggttggcctc ctcgacgagc agctcgcccg ccgggctgca
      901 cggcgggtgc agggcgtcga cgccgacccg ggctggacgg tcgccgtcga cggcggcgac
      961 acccggcccc ggcaggtcgc cgagacgctg ctgaccgtcg cggacggcct gctcggcacg
     1021 cggggcgtcc gcgaggagga cggggcggga cccgcgccgg ccaccgtggc ggccggcgtc
     1081 tacgacgacc ggagcgagac cgtccccctg ctgcccgggc cgggctggac cgggctcgag
     1141 gtggcagcgg gcgccggacg cgagcgccgc gtgctcgacc tgcggtcggg ggtcctgtgg
     1201 cgggagcggg ccgatgacgg cgctccgctg cgcacgctgc ggttctcctc cctggcccgg
     1261 cccggcgtgg tcgcgttgcg cgccgagggc ggcccagccg tcctccggcc cggcccggcc
     1321 ctgctgcctc ccggcggcga ggcaggcttc gagcagggcc ggctcgggga cgtgacctgg
     1381 gcccgcaccc gggcccggcg ggacgggggc atcaccgcag cggcccgcca gcgggaaggc
     1441 ggcggcgacc gggcaacggt cgagcgcatc gcggcctatg tcgccgaccc caccggggca
     1501 cccgggcccg aggaggccgc cgagcgcctt cgggccgccg aggcggtcgg cttcgaccag
     1561 ctcctggccg agcaccgccg cgcctgggcc gctcgctggg cggacgcgga ggtgacgatc
     1621 gagggcgacc cacggtccga gcaggccgtc cgcttcgccc tgttccacct cctggcgaac
     1681 gtcgccgacc ggggggaggc cgcggtgggc gcccgcggcc tgtccggccc ggcctacgcg
     1741 gggcacgtgt tctgggacgc cgacgtgttc gtcctcccga cgctcgccgc cacgcacccg
     1801 gcggccgccc gggcgatgct cgagtaccgg gtgcggcggc tcccggccgc ccggcgcatc
     1861 gccgccgacc tgggctacgc cggggcgcgc ttcccctggg agtcggccgc cgacggcaac
     1921 gaggtgacgc cggcgaccac cgtcgacgag cgcggcgagg tgatcgag
//
LOCUS       DASPYY010000423         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2740756, whole genome shotgun sequence.
ACCESSION   DASPYY010000423 DASPYY010000000
VERSION     DASPYY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740756"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VEP73_06575"
     CDS             complement(<1..558)
                     /locus_tag="VEP73_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HYT26129.1"
                     /translation="MDGTAGGERLTSAEASARLGVKPETLYAYVSRGLLRSERAANGR
                     TSSFDRDEVERLARRGRRREPRGAPELVIESALTAIQHGRLWYRGLDATELARTRTFE
                     EVASWLWTGRLGGGASEPRGWRADPAAVAVAAACQAALPAGTLPLDRLRVVAAALAAA
                     DELRFELHPAATVATGQALVAGLVDA"
     gene            672..1901
                     /locus_tag="VEP73_06580"
     CDS             672..1901
                     /locus_tag="VEP73_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26130.1"
                     /translation="MPRIHFLDVTNRDGVQTARVNLSKFGKTMVNFYLGKLGVRQSEV
                     GFPALFHEVPYIRANLALARAGAFGDLRLSGWARAVPGDVEQAAPLGLAHMNLSISTS
                     DQMIANKFNGRLDRAAVLREMVKATTAAKAAGVETVGVNAEDGSRTDDGYLAEFAHAA
                     REAGADRVRYCDTIGGETPGHVRERFAHLAADAGIPIETHCHNDLGMAVANSVAGALG
                     DLDAGQDAWVNTCVNGIGERAGNADLLACILAFEHGFGVAGRVELGDDLDLSWARRLG
                     LWASDAFGQPLPLGQPGVGRNAFAHESGIHADGALKDRHNYELYDDETLGPFPHDHAR
                     REGRVVLTGEYGGRAGFRHVLGTLGVEVAADDEALVFKLVQLTNAATGRPLTDDELRL
                     LAAYPRELALLYPGLLS"
     gene            complement(1952..3283)
                     /locus_tag="VEP73_06585"
     CDS             complement(1952..3283)
                     /locus_tag="VEP73_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYT26131.1"
                     /translation="MRRRDFLAGTGLAAGAGALAGLAGCEPHRSVRPATPAATAPGPA
                     SLDDWGAVRDQFDLDPGYLHFAAFVLASHPRPVREAIDAHRRGLDADAAGYLRANELR
                     LEGDVLAAAAAYLGARPEELALTDSTTMGLGLLYGGLRLRAGQEVVATTHDFYSTHES
                     LRLAAERAGAPFRKVPLYHDLGSASADEVAGRARRAVGPRTRVLAVTWVHSSTGLKLP
                     VRAIADALDGLNRDRDAADRVLLCVDAVHALGVEPDALPGLGCDFLVAGCHKWLFGPR
                     GTGIVWGAPAAWPAVAATIPTFDRRGIGAWIAGDAPGDLPPGAAMTPGGYHSFEHRWA
                     LADAFRFHARIGKERVAQRTQALARQLKEGLRAMAHVRLATPLAEELSAGIVCFDVDG
                     RNAAEVRDHLLADKVVASLTPYAVEHVRLGPSMVTSPGDVDRALGSIRALA"
     gene            complement(3406..>3990)
                     /locus_tag="VEP73_06590"
     CDS             complement(3406..>3990)
                     /locus_tag="VEP73_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT26132.1"
                     /translation="PPPYDPGKAKQLLAEAGYPNGLTLNYVGVNTGASKQVDTVVQAQ
                     LARAGIKLNVRQLTNSAHYTDQLQIPAKRTQHQIGNAGWCPDFAGDGTRSFIVPNFDG
                     RSIQPSGNQNYGEYNNAEVNSMIDKALAEPDRAKRAELWSALDKRIMQDAVWVPYLYD
                     KSPWFWSARTKNWQFTPWFAEPDLTAIWLSPTTP"
BASE COUNT          507 a         1474 c         1488 g          521 t
ORIGIN      
        1 ggcgtcgacg agcccggcga cgagcgcctg gccggtcgcc acggtggccg ccgggtggag
       61 ctcgaagcgc agctcgtcgg ctgcggcgag cgcggcggcg acgacccgca ggcggtccag
      121 cggcagggtg ccggcgggca gcgccgcctg gcaggcagcc gccaccgcga ccgcggcggg
      181 gtcggcccgc cagcccctgg gctcggaggc cccgccgccg agccggcccg tccacagcca
      241 ggaggcgacc tcctcgaagg tccgcgtgcg cgccagctcc gtcgcgtcga ggccgcggta
      301 ccagagcctg ccgtgctgga tggcggtcag cgccgactcg atcaccagct ctggcgcgcc
      361 gcggggctcg cggcggcggc cccggcgggc gagccgctcg acctcgtccc ggtcgaacga
      421 gctggtgcgc ccgttggcag cccgctcgct gcgcaagagc ccccggctga cgtaggcgta
      481 gagcgtctcg ggcttgaccc ccagccgcgc cgacgcctcc gcgctggtca gtcgctcgcc
      541 tcccgccgtc ccgtccatgg cggccgcccc gctcccacac tcgcttacat tgattacatg
      601 atcaacgttg acgaagcgta gcaggggtgc caccatcggg tccagagcga cccacttccc
      661 ggagctgcgc catgcctcgc atccacttcc tcgacgtcac caaccgcgac ggcgtccaga
      721 cggcccgtgt caacctctcg aagttcggca agacgatggt caacttctac ctcggcaagc
      781 tcggcgtccg gcagtccgag gtcgggttcc cggcgctgtt ccacgaggtg ccgtacatca
      841 gggcgaacct ggcgctggcc cgggccgggg cgttcggtga cctgcggctg tcgggatggg
      901 cccgggccgt gcccggcgac gtcgagcagg ccgccccgct cggcctcgcg cacatgaacc
      961 tctcgatctc gacctccgac cagatgatcg ccaacaagtt caacggccgc ctggaccggg
     1021 ccgccgtcct ccgcgagatg gtgaaggcga ccacggcggc caaggcggcc ggggtcgaga
     1081 ccgtcggcgt caacgccgag gacggctccc gcaccgacga cggctacctg gccgagttcg
     1141 cccacgcggc cagggaggcc ggggccgacc gtgtccgcta ctgcgacacc atcgggggcg
     1201 agacgccagg gcacgtccgg gagcgcttcg cgcacctggc cgcggacgcc ggcatcccga
     1261 tcgagacgca ctgccacaac gacctcggca tggcggtcgc caactcggtc gccggcgcgc
     1321 tcggcgacct cgacgccggc caggacgcct gggtgaacac ctgcgtcaac ggcatcggcg
     1381 agcgggcggg caacgccgac ctgctcgcct gcatcctcgc gttcgagcac ggcttcggcg
     1441 tggccggccg ggtcgagctc ggcgacgacc tcgacctgtc ctgggcgcga cgcctcggcc
     1501 tctgggcgag cgacgcgttc ggccagccgc tgccactcgg ccagccgggc gtcggccgca
     1561 acgccttcgc ccacgagtcc ggcatccacg ccgacggcgc gctcaaggac cgccacaact
     1621 acgagctgta cgacgacgag acgctcgggc cgttcccgca cgaccacgcc cggcgcgagg
     1681 gccgggtcgt gctgaccggc gagtacggcg ggcgggccgg gttcaggcac gtgctcggca
     1741 ccctcggggt cgaggtcgcg gccgacgacg aggcgctcgt cttcaagctc gtgcagctga
     1801 ccaacgcggc gacgggccgt ccgctcaccg acgacgagct gcgcctgctc gcggcctacc
     1861 cccgggagct cgccctcctc tatcccggcc tgctgtcctg atccccgatc cgccgtcccc
     1921 gatccgccgc ggcgcaggga gggcccggtg ctcaggcgag cgcccggatc gagccgagcg
     1981 cgcggtcgac gtcgcccggc gaggtcacca tgctggggcc gaggcggacg tgctcgacgg
     2041 cgtagggcgt gaggctcgcc acgaccttgt cggcgaggag gtggtcgcgc acctcggccg
     2101 cgttgcggcc gtcgacgtcg aagcagacga tcccggccga gagctcctcg gcgagcgggg
     2161 tcgcgagccg gacgtgcgcc atcgcccgga gcccctcctt gagctggcgg gcgagcgcct
     2221 gggtgcgctg ggcgacgcgc tccttgccga tccgcgcgtg gaagcggaac gcgtccgcga
     2281 gcgcccagcg atgttcgaac gagtggtacc cgcccggggt catcgcagcg ccgggcggga
     2341 ggtccccggg cgcgtccccc gcgatccagg ccccgatccc gcgccggtcg aacgtgggga
     2401 tggtcgccgc caccgcgggc caggcggccg gcgcgcccca gacgatcccc gtgccccgcg
     2461 ggccgaacag ccacttgtgg cagccggcga cgaggaagtc gcagccgagc cccggcaggg
     2521 cgtccggctc gaccccgagc gcgtggaccg cgtcgacgca cagcagcacg cggtcggcgg
     2581 cgtcccggtc ccggttgagc ccgtcaaggg cgtcggcgat ggcgcggacc ggcagcttga
     2641 gcccggtgct cgagtgcacc caggtgaccg cgagcacgcg ggtgcgcggg ccgacggcgc
     2701 gcctcgccct gcccgcgacc tcgtcggcgg aggccgagcc gaggtcgtgg tagagcggca
     2761 ccttgcggaa cggcgcgccg gcccgctcgg cggccagccg cagcgactcg tgcgtggagt
     2821 agaagtcgtg ggtggtggcg acgacctcct ggcccgcccg cagccgtagg ccgccgtaga
     2881 ggaggccgag gcccatcgtc gtcgagtccg tcagggcgag ctcctcgggc cgggcgccga
     2941 ggtaggcggc cgccgcggcc agcacgtcgc cctcgagccg gagctcgttg gcccggaggt
     3001 agccggccgc gtcggcgtcg aggccccggc ggtgggcgtc gatcgcctcg cgcaccggcc
     3061 gcgggtgcga ggccaggacg aacgcggcga agtgcaggta gccggggtcg aggtcgaact
     3121 ggtcgcggac ggcgccccag tcgtcgaggc tcgccggtcc cggcgcggtg gccgccgggg
     3181 tcgccggacg gacggaccgg tgcggctcgc agccggcgag cccggcgagc gcgccggccc
     3241 cggcggcgag cccggtcccg gcgaggaagt ccctgcgtcg catgccatca ccgcctctcg
     3301 ggcaagtgtg gtcttagcaa gtgtggtcga agcgccgtcg cggcgccagg accgcgaccg
     3361 ccgaggggcc gcacgcggcg gcccctcggc ccgttggcgc ggctgctacg gcgtggtcgg
     3421 gctgagccag atcgcggtca ggtcgggctc ggcgaaccag ggggtgaact gccagttctt
     3481 ggtcctcgcc gaccagaacc acgggctctt gtcgtagagg tacggcaccc agacggcgtc
     3541 ctgcatgatc cgcttgtcga gggccgacca cagctcggcg cgcttggccc tgtcgggctc
     3601 tgccagcgcc ttgtcgatca tcgagttgac ctcggcgttg ttgtactcgc cgtagttctg
     3661 gttgcccgac ggctggatcg agcggccgtc gaagttcggc acgatgaacg agcgcgtccc
     3721 gtcgcccgcg aagtccgggc accagcccgc gttcccgatc tggtgctgcg tccgcttggc
     3781 cgggatctgg agctggtcgg tgtagtgggc cgagttcgtg agctgccgca cgttcagctt
     3841 gatgccggcc cgcgcgagct gggcctgcac gaccgtgtcg acctgcttgc tggccccggt
     3901 gttcacgccg acgtagttca gcgtgagccc gttcgggtag ccggcctcgg ccaggagctg
     3961 cttggccttg cctgggtcgt agggtggagg
//
LOCUS       DASPYY010000424         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2460476, whole genome shotgun sequence.
ACCESSION   DASPYY010000424 DASPYY010000000
VERSION     DASPYY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460476"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEP73_06595"
     CDS             <1..800
                     /locus_tag="VEP73_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26133.1"
                     /translation="LTAIAFGTVVETTRKRRARHLDDFRDVRMLSQEDIAALGGDLSS
                     LETTMQAIDDEDDAALRDYAVAHEWYEKGIERFERAARPEDLAAVSSALETARWHMTS
                     ARSVVEGHGRLRRLPPCFFDTRHGPSVNDVGWMPPRGSARPVPACETCMHLVEDNVQP
                     PVRLVRTNGALVPFHDAPPHFESWFGGYFGGTAHALVEGFALGRALDDGFAGGLNTFG
                     GGHGYMPPSYADSGILDSGGGDIGGARRFDEGDYVRGDFGPESREPP"
     gene            882..>2240
                     /locus_tag="VEP73_06600"
     CDS             882..>2240
                     /locus_tag="VEP73_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26134.1"
                     /translation="MITSVPFSRPRRLLAALTAGLALTVLLAGPAAAGQYVDPAVNGL
                     QRDHVYLDPGSEPVVSPEAADKLRDQIAGTSRPVYVAVLPKEALAEGDVSSLLEQISS
                     RVHKTGVYAVLAGDPKSGQGGFRAGASQDAGYRSGVIPAAATAAFRAHRDDPEALLTD
                     FVDRVAAAPQGNAAGQSQSAGTLPPGDEGGSGGGGGGAAAAVLLGILGIVVVGGGLLF
                     SRSRRRRREEERRQLEQVRSTANEDLIALSNDLSSIDLDVNMPGANPDAKHHYASAVA
                     SYERAADALDRARRPQDLAPVSTALEEGRYGVSAARALLEGRQLPERRPPCFFDPRHG
                     PSVRDVTWTPPGGAPRQVPACAADAERVERGEEPQTRQVMVGDRREPFWNAPGYYAPW
                     YGGYYGGFGGGGGLLTGLLLGTALSGGFGGWGGGGYGAGYDAGFDQGQDAGDGGDGGG
                     WGD"
BASE COUNT          304 a          833 c          841 g          262 t
ORIGIN      
        1 tgctcaccgc gatcgcgttc ggcacggtcg tcgagaccac gaggaagcgc cgtgctcggc
       61 atctggacga cttccgcgac gtccgcatgc tctcccagga ggacatcgcc gcgctcggcg
      121 gggacctgtc gagcctcgag accaccatgc aggcgatcga cgacgaggac gacgccgccc
      181 tgcgcgacta cgccgtcgcc cacgagtggt acgagaaggg catcgagcgg ttcgagcggg
      241 cggccaggcc agaggacctc gccgcggtca gctccgccct cgagaccgcc aggtggcaca
      301 tgacgtcggc gcgctcggtg gtcgagggcc acgggcggct gcgccggctc cctccctgct
      361 tcttcgacac gaggcacggg ccctcggtca acgacgtcgg ctggatgccg ccccggggca
      421 gcgcccgccc ggtgcccgcc tgcgaaacct gcatgcacct ggtcgaggac aacgtccagc
      481 cgccggtacg gctcgtcagg acgaacggcg ccctggtccc gttccacgac gcgccgcccc
      541 acttcgagtc ctggttcggc gggtacttcg gcggcaccgc ccacgccctg gtcgaggggt
      601 tcgcgctggg ccgggcgctc gacgacggct tcgccggcgg gctcaacacc ttcggggggg
      661 gccacggcta catgccaccc tcgtatgccg actccggcat cctggactcc ggcggcggcg
      721 acatcggcgg cgcgaggcgg ttcgacgagg gcgactacgt gcgcggcgac ttcgggcccg
      781 agtcgcgcga gcccccctga cgccgggtgg cgtccgcggc cgtcgtatcc tcccgccgga
      841 agggcggaca atggggggag catcgaggcc caggagcgac catgatcacc agcgtcccgt
      901 tctcacgacc ccgccggctg ctggccgcgc tgaccgccgg cctggccctg acggtcctgc
      961 tggccggccc cgccgccgcg ggccagtacg tcgacccggc ggtgaacggc ctgcagcgcg
     1021 accacgtcta cctcgacccg ggcagcgagc ccgtggtcag cccggaggcg gcagacaagc
     1081 tccgcgacca gatcgcgggc acgagccggc ccgtctacgt cgccgtcctg cccaaggagg
     1141 cgctcgccga gggcgacgtc agcagcctcc tcgagcagat ctccagccgg gtccacaaga
     1201 ccggcgtcta cgcggtcctg gcgggcgacc cgaagagcgg gcagggtggc ttcagggccg
     1261 gggccagcca ggacgccggg taccgcagcg gcgtgatccc ggcagcggcc acggccgcgt
     1321 tccgggctca cagagacgat cctgaggccc tgctgacgga cttcgtcgac cgggtcgcgg
     1381 cggcgcccca ggggaacgcg gccgggcagt cccagagcgc cggcacgctg ccgcccgggg
     1441 acgagggcgg atccggcggt gggggcggcg gggccgcggc agcggtcctc ctcggcatcc
     1501 tcggcatcgt cgtggtagga ggtggcctgt tgttctctcg gtcaaggcgg cgcaggcgcg
     1561 aggaagagcg gcgccagctc gagcaggtgc ggtccacggc gaacgaggac ctgatcgccc
     1621 tctccaacga cctgtcgagc atcgacctcg acgtgaacat gccaggcgcg aaccccgacg
     1681 ccaagcacca ctacgcgagc gcggtcgcga gctacgagcg tgccgccgac gcgctcgacc
     1741 gcgcgaggag gccgcaggac ctcgccccgg tcagcaccgc gctcgaggag gggcggtacg
     1801 gggtctcggc ggccagggcg ctcctcgagg gcaggcagct ccccgagcgc cggcccccct
     1861 gcttcttcga ccccaggcac gggccctcgg tccgcgacgt cacctggacc ccgcccggcg
     1921 gcgccccccg gcaggtcccg gcctgcgccg ccgacgccga gcgggtcgag cgcggcgagg
     1981 agccgcagac ccgccaggtg atggtcggcg acaggcggga acccttctgg aacgcgcccg
     2041 gctactacgc cccgtggtac ggcgggtact acggcggctt cggcgggggc ggcggcctgc
     2101 tcaccggcct gctgctcggc acggcgctga gcggcggctt cggcggctgg ggcggcggcg
     2161 ggtacggggc cggctacgac gccggcttcg accagggcca ggacgccggg gacggcgggg
     2221 acggcggcgg ctggggcgac
//
LOCUS       DASPYY010000425         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_914432, whole genome shotgun sequence.
ACCESSION   DASPYY010000425 DASPYY010000000
VERSION     DASPYY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914432"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VEP73_06605"
     CDS             complement(<1..1278)
                     /locus_tag="VEP73_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT26135.1"
                     /translation="MLEEVRITGLGVIDDAVLELSAGFTALTGETGAGKTMVVTALGL
                     LFGGRADPARVRPGAARAAVEGRLLVTPEVAREVEEAGGDLDDNGSSLLLSRSVSAEG
                     RSRAFAGGHSVPVSLLQVLADDLVAVHGQSDQQQLLKPGRQRDALDRFGGPELAAALA
                     GYRRVYHRHRAVRDELDALTRAERERAAEVETLRLGLAEVEATAPEAGEDSALLAEEE
                     KLANADALHAAATTAHEALLGDPASASVDASDVVTLLGLAVRALEPVRSHDPALAGLA
                     DRLSEASYLISDVAGELASYTESLDSDPARLAAVQERRAALGRLIRAYAAASGDLAGV
                     LDWAKQAGSRLAELEGDDDKIAALAAEEAALRSEVTSLAARLTGLRSAAAERFASEVT
                     AELTALAMPHASLTAVVSELDAPGPYGADDVEIR"
     gene            complement(1271..>1648)
                     /gene="ppnK"
                     /locus_tag="VEP73_06610"
     CDS             complement(1271..>1648)
                     /gene="ppnK"
                     /locus_tag="VEP73_06610"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852520.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HYT26136.1"
                     /translation="STAYAFSAGGPVVWPEVEALLMVPISAHALFAPPMVVSPRSVLA
                     VELIVASETSGAVLWCDGRRKVDLPPGARVEVRRGALPVLLARLQAPDTRGGGFTDRL
                     VAKFGLPVAGWRGRTWEDAGKHA"
BASE COUNT          215 a          665 c          583 g          185 t
ORIGIN      
        1 cctgatctcc acgtcgtcgg cgccgtaggg gccgggcgcg tccagctcgc tgaccacggc
       61 ggtgagagac gcgtgcggca tggccagcgc ggtcagctcg gcggtcacct cggacgcgaa
      121 ccgctccgcc gccgccgaac gcaacccggt cagccgcgcc gcaagcgagg tcacctcgga
      181 tcgcagggcg gcctcttccg cagccagcgc ggcgatcttg tcgtcgtccc cctccagctc
      241 ggccaggcgc gacccggcct gcttggccca gtcgagcacc ccggccaggt cgcccgaggc
      301 ggccgcgtag gccctgatca gccggccgag ggcggcccgg cgctcctgga ccgcggccag
      361 ccgggccggg tccgagtcca gcgactcggt gtaggaggcc agctccccgg ccacgtcgga
      421 gatcaggtag ctggcctcgg agagccggtc ggccaggccg gccagggccg ggtcgtgcga
      481 gcggaccggc tcgagcgcgc ggacggccag gccgagcagc gtcaccacgt cggacgcgtc
      541 gaccgaggcc gaggccggat cgccgagcag cgcctcgtgc gcggtggtgg ccgcggcgtg
      601 cagcgcgtcg gcgttcgcca gcttctcctc ctcggccagc agggccgagt cttcgcccgc
      661 ctccggggcg gtcgcctcga cctcggccag gccgagccgc agcgtctcga cctcggccgc
      721 gcgctcccgc tccgcccggg tcagcgcgtc gagctcgtcg cggaccgcgc ggtgccggtg
      781 gtacaccctc cggtagccgg ccagggcggc ggccagctcg ggcccgccga acctgtccag
      841 cgcgtcccgc tgccggcccg gcttgagcag ctgctgctgg tcggactgcc cgtgcacggc
      901 caccaggtcg tcggccagca cctggagcag cgataccggc accgaatgtc cgccggcgaa
      961 cgcccgggac cggccctcgg ccgacaccga gcggctgagc aggagcgagg acccgttgtc
     1021 gtcgaggtct cccccggcct cctcgacctc gcgggctacc tccggggtga ccagcaggcg
     1081 cccctccacc gcggcccgcg ccgcgccggg ccggacccgc gccgggtccg cccggccgcc
     1141 gaacagcagc ccgagcgccg tcacgaccat ggtcttcccg gcaccggtct cgccggtcag
     1201 cgcggtgaag ccggccgaga gctcgagcac cgcgtcgtcg atgacgccga ggccggtgat
     1261 ccggacttcc tcaagcatgc ttgcctgcgt cctcccaggt acggccgcgc cagcccgcca
     1321 cgggcaggcc gaacttggcc accagccggt ccgtgaagcc cccgccccgg gtatccggcg
     1381 cctgcagccg tgcgagcagg accggcagcg ccccgcggcg gacctccacc cgggccccgg
     1441 gtggcagatc gaccttacgc cgaccatcgc accacaaaac cgcccccgat gtctcggacg
     1501 ccacgatcag ctcgaccgcg agcaccgatc gcggcgacac caccatgggc ggggcgaaca
     1561 gcgcgtgcgc gctgatcggc accatgagca gcgcctctac ctcgggccag accaccggcc
     1621 cgcccgcgga gaacgcgtag gcggtcga
//
LOCUS       DASPYY010000426         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_634832, whole genome shotgun sequence.
ACCESSION   DASPYY010000426 DASPYY010000000
VERSION     DASPYY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634832"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VEP73_06615"
     CDS             <1..440
                     /locus_tag="VEP73_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26137.1"
                     /translation="LVGEVTDVHAAGSRAGSARVPGDVDEVTVATLRFATGAVGTVSS
                     TCLLGRLHRAGIHLYAEGLALELSETELVVDDGGGSLVTYRARADARPLPDRDFVDAV
                     RGGPNRVRVPYAEALGTHRLACAITRSAESGRTVELPGERTRA"
     gene            433..1560
                     /locus_tag="VEP73_06620"
     CDS             433..1560
                     /locus_tag="VEP73_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HYT26138.1"
                     /translation="MPEPDRPVPAAGAWRVDVLELQAPGRVALTSREEGPLEGGRFRL
                     ETCYSGLSAGTELTFYKGTNPYLHAAWDPSLGVFRAGRPAQTFPISPFGYMEVGRVCA
                     SRTRAAAEGDLVAMAYGHKTGHTVDPAADRFIPLPGDLDPLLGIYLAHMGPICANGLL
                     HAAADVAGPGTHSLGDGVRGRNVLVTGGGVIGLLVGLLARHHGAAAVAVADATPERLR
                     AADRLGLDPIDDGDGDAWLVAKERWRHDPRDAGADVVFQCRGRTASLATALRSLRPQG
                     TVIDLAFYQGGAPEVRLGEEFHHNGLSVRCAQVSRVPRGLAHLWDRRRLAAETLDLLR
                     AHGPAIREHLVTDVVPLADAPALFADVAARRRHVLQAVFAC"
     gene            complement(1573..>2392)
                     /locus_tag="VEP73_06625"
     CDS             complement(1573..>2392)
                     /locus_tag="VEP73_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26139.1"
                     /translation="HPEFYAGFDGRGRVFFNCVSETQRRNAPPALGAQILGAVHLAVP
                     VQAHEFQPVKGDRFLTLGRFTHAKGADVAARLCRRLGLPLDMAGPVGGVPEPGRLLRE
                     LEDPDSPLHGYADVRYYLDAVRPCEDGSRIAWIGTAGGARKQALVGSAKAVLMPIRWD
                     EPGGTAAIEALACGTPVIAMRRGALAEIVEHGVNGFLADDEDDFARWMRRAGEIDPQA
                     CRRSVEDRFSAPAMADAYLRLYEEVLARTAHHQTNHATTPTDLGWSRPRSGAHA"
BASE COUNT          277 a          906 c          910 g          299 t
ORIGIN      
        1 ccctggtcgg cgaggtcacc gacgtccacg cggccgggtc gcgggccggg tcggccaggg
       61 tccccggcga cgtcgacgag gtcacggtcg ccaccctgcg gttcgcgacc ggcgcggtcg
      121 gcacggtgtc gtccacctgc ctgctcggcc ggctgcatcg ggcaggcatc cacctgtacg
      181 cggaggggct ggcgctcgag ctgtcagaga ccgagctggt cgtggacgac ggcgggggga
      241 gcctggtcac gtaccgggcg cgggccgacg cgcggcctct gccggaccgg gacttcgtcg
      301 acgcggtacg gggtggtccg aaccgcgtcc gcgtgccgta cgccgaggcg ctcgggaccc
      361 accggctcgc ctgcgccatc acccgctccg cggagtccgg ccgcaccgtg gagctgccag
      421 gggagcgcac gcgtgcctga gccggaccgg ccggtgccag ccgccggggc ctggcgggtg
      481 gacgtgctcg aactccaggc cccgggccgc gtcgccctca cgagccgcga ggaggggccg
      541 ctcgagggcg gccggttccg gctcgagacc tgctacagcg gcctgtcggc cggcaccgag
      601 ctgacgttct acaagggcac gaacccgtac ctccacgcgg cctgggaccc gagcctcggc
      661 gtgttccgtg ccggccggcc ggcgcagacg ttcccgatca gcccgttcgg ctacatggag
      721 gtcggccggg tgtgcgccag ccggacccgc gccgccgcag agggcgacct ggtcgcgatg
      781 gcctacggcc acaagaccgg ccacacggtc gaccccgcgg ccgaccgctt catcccgctg
      841 cccggcgacc tcgacccgct gctcggcatc tacctggcac acatgggtcc gatctgcgcg
      901 aacgggctcc tgcacgccgc cgccgacgtc gccgggccgg gcacgcactc gctcggcgac
      961 ggcgtccggg ggcggaacgt gctggtcacc gggggcgggg tcatcggcct gctggtgggg
     1021 ttgctggcgc gccaccatgg cgcggccgcg gtggcggtgg cggacgcgac ccccgagcgg
     1081 ctgcgcgcgg ccgaccggct cggcctcgac ccgatcgacg acggcgacgg cgacgcctgg
     1141 ctggtcgcga aggagcgctg gcggcacgac cccagggacg cgggcgccga cgtggtgttc
     1201 cagtgccgcg gccggaccgc cagcctcgcg accgccctgc gcagcctgcg gccccagggc
     1261 accgtcatcg acctggcctt ctaccagggc ggcgcgcccg aggtccggct cggcgaggag
     1321 ttccaccaca acggcctctc ggtccgctgc gcgcaggtct cccgggtgcc gagggggctc
     1381 gcccacctct gggaccgcag gcggctcgct gccgagaccc tcgacctgct ccgcgcccac
     1441 gggccggcca tccgggagca cctggtcacc gacgtcgtcc ccctggccga cgccccggcg
     1501 ttgttcgccg acgtggccgc ccgcaggcgc cacgtgctcc aggccgtctt cgcctgctga
     1561 gccctgaggg acctacgcgt gggcgcccga gcgggggcgg ctccacccga ggtccgtggg
     1621 cgtggtcgcg tggttggtct ggtggtgcgc ggtccgggcc aggacctcct cgtacagccg
     1681 caggtaggcg tctgccatgg ccggggcgga gaagcggtcc tcgaccgacc gccggcacgc
     1741 ctgcgggtcg atctccccgg cccgccgcat ccaccgcgcg aagtcgtcct cgtcgtcggc
     1801 caggaagccg ttcacgccgt gctccacgat ctcggcgagg gcgccgcggc gcatggcgat
     1861 cacgggcgtg ccgcaggcga gcgcctcgat ggcggccgtg ccgcccggct cgtcccacct
     1921 gatcggcatg agcaccgcct tggcgctccc gaccagggcc tgcttgcggg ccccgccggc
     1981 ggtcccgatc caggcgatgc ggctgccgtc ctcgcagggg cgcaccgcgt cgaggtagta
     2041 gcgcacgtcc gcgtagccgt gcagcgggct gtcgggatcc tcgagctcgc ggaggagccg
     2101 gccgggctcc gggacccccc cgaccgggcc cgccatgtcg aggggcaggc ccagccgcct
     2161 gcacaggcgg gccgcgacgt cggcgccctt ggcgtgcgtg aagcgtccga gggtgaggaa
     2221 gcggtcgccc ttcaccggct ggaactcgtg ggcctggacc gggacggcga gatgcaccgc
     2281 gccgaggatc tgcgcgccga gagcgggcgg cgcgttgcgg cgctgcgtct cggagacgca
     2341 gttgaagaac acgcggcccc ggccgtcgaa cccggcgtag aactcgggat gc
//
LOCUS       DASPYY010000427         8453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1617276, whole genome shotgun sequence.
ACCESSION   DASPYY010000427 DASPYY010000000
VERSION     DASPYY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617276"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VEP73_06630"
     CDS             <1..1046
                     /locus_tag="VEP73_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYT26140.1"
                     /translation="WRHRSERYGEKLATPDTSVGDLIGDVDPIKVAEGRTLGDPETVH
                     YGLVPRTNRGIFSVNELPDLAERIQVALLNVLEERDIQVRGYQLRLPLDLLLVASANP
                     EDYTNRGRIITPLKDRFGAEVRTHYPLRLDDELRLVRQEAVLTWEGSPLGKPVLPDHV
                     VEVIARFTRGVREAPQIDARSGVSARFAIAAAETAAASAVRRAAVTGEETPVVRVVDL
                     PAVVPTSLGKVEFDQLEEGRELDLLKHLLRRAVADTYRSRLGGQDVSGLLRRFEDGGT
                     VETGDLVPAAELLRRVGPVPGLAQLLRRLGDDEGAETPGLAAAALEFTMEGLHLGKRL
                     AKEELPGRAVYGG"
     gene            1043..3058
                     /locus_tag="VEP73_06635"
     CDS             1043..3058
                     /locus_tag="VEP73_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT26141.1"
                     /translation="MSIRYGRWRGGPDPLEPPFDVAAALDRMGERVLAGDRTGEALSS
                     LLRQGMPGLRGLRDLLRQVRERRREARGRGALDGTLERVRELLERALREEREALAADP
                     SDAARLAGAELDQLPGDTAGAVRQLADREWRSPEAAATYEEIRDLLRREVLDTQFRGM
                     KQALMGAMPEDLERVRDMLADLNAMLDADARGEDTTAAFDQFMQRYGDFFPEGPENLD
                     ELVDSLARRATAASRLLGSLSPEQREELSALMQAAMADLGLERELDRLGRALEWRRPD
                     LRWGQGVPMRGGQPLGLGDATTALEELADLEALEASLGQDYPGASLDDVDLEAVERAL
                     GRGAAADVELLRTLERELAEQGYLTREHGELQLTPKAIRRIGATALRRIFSSLDSRAR
                     GDHDVASAGTSGEPTGSSRPWTFGDEQPLDVVGTVRNAVLRGGALPAGGAGSAPPDPP
                     GRLLGGRGPARVVLEAGDFEVLDTERRTSAAICLLVDLSYSMLLREAWGAAKSTALAL
                     QSLVSTRYPQDALSIVGFSSVARTLRPRELADLAPNMDQQGTNLQHALLLAGRHLDRH
                     PDAEPVVLVVTDGEPTAHLQPGGHPYFEWPPSQETIELTMAEVLRLTRRGATINVFML
                     DTEPRLVRFVQALARLNGGRVFTPSSDRLGDYVVNDYLRARRGRRAR"
     gene            complement(3119..3325)
                     /locus_tag="VEP73_06640"
     CDS             complement(3119..3325)
                     /locus_tag="VEP73_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYT26142.1"
                     /translation="MTVGTVKWFNAEKGYGFIAPEQGEDVFVHFSAIQASGYRSLNEG
                     QQVEFDITQGPKGAQAANVRPLEQ"
     gene            complement(3577..4404)
                     /locus_tag="VEP73_06645"
     CDS             complement(3577..4404)
                     /locus_tag="VEP73_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26143.1"
                     /translation="MTEEQELDRLFGLPLDAFVNARAEAARALRQAGDAAAAQRIAAL
                     RKPSVSAWAVNQLARRHASRLGRLLERGERLRAAHDAVLAGAGRSGLAKAARDEREAV
                     QELVEAAAGELAAAGRPASEAVRERIAGSLHAAAREPRAAALAREGRLARDLDPPGFG
                     GGAWPVGVAEAEAAGPAGRRGVEAGRVEDAEGNERLLAGRRARDEARRQARAAADAAI
                     LARRQAEQLRHAADRAEQAAIRAREAADDAVQAERRTRERAARTAAELADAEARLVG"
     gene            4715..5146
                     /gene="dut"
                     /locus_tag="VEP73_06650"
     CDS             4715..5146
                     /gene="dut"
                     /locus_tag="VEP73_06650"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYT26144.1"
                     /translation="MDLPVHRLEPGLPLPAYAHPGDAGADLFAAEDVKLPPGGRAVVG
                     TGMAVAIPEGFAAFVHPRSGLAARHGVTVANAPGTVDAGYRGEVKVVLLNTDPAEPHV
                     VRRGDRIAQLVVQPVVRARFLDVRELPPAARGGGGFGSTGP"
     gene            5201..6088
                     /locus_tag="VEP73_06655"
     CDS             5201..6088
                     /locus_tag="VEP73_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26145.1"
                     /translation="MDPRDGRGGGRDQPWGERLADTASSPGGDPTAGRLIRDAIGVVR
                     GRLSDVARGGIQPLLDEAAAQVRPATLDELRTRYPGLTEDEIARKLIDRSARTAVGVA
                     LAIGGILAAQEAVAVASSPVPPAAGAALGTVGVTALAEVLVLFVIEAKLRADLGALAG
                     QRVPTPRELVAGVVGEVQAAGGLGRLRRRSLRRALPEAAVLRAATRIARLMPARFARI
                     VIPEVVAPLVGAAWAARLASQQLRATGERCWGELRLLPRTTVSWGPSTWNGGNPSGPR
                     GPGDSRSLPPPDPTWRPPS"
     gene            complement(6119..6964)
                     /locus_tag="VEP73_06660"
     CDS             complement(6119..6964)
                     /locus_tag="VEP73_06660"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYT26146.1"
                     /translation="MTDRDQLLVTTDWLAEHLDDPGIRVVEVDEDTDAYNRGHIPGAV
                     SVNWNTDLQDPLRRDFVSPEAFAKLQDSLGIGNDTTVVLYGGNNNWFAAYAYWYYKVY
                     GHENVKLVDGGRKAWELEDRPLTTDSPAVEPSSGYKAGEPDVSIRAFRDQVLSDYVGR
                     DGAALVDVRSPEEYRGEKLAPDHLPQEAAQRPGHIPGAANVPWAKAVDGDTGRFLTDD
                     RLAELYGGKGVTPDKDVVAYCRIGERSAHTWFVLHELLGYPRVRNYDGSWTEWGSLVN
                     VPIER"
     gene            7447..8025
                     /locus_tag="VEP73_06665"
     CDS             7447..8025
                     /locus_tag="VEP73_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYT26147.1"
                     /translation="MAFDPTPDDALVIIDVQRDFCPGGSLAVPGGDEVVPVVNRLAPR
                     FATVVTTHDTHPADHCSFAARGGPWPPHCVEGTPGWDPHPGLDVRPDFQVFKGRDRDQ
                     DGYTGWTPALADFLSRRGARRVVAAGLALDYCVQATALDARAAGFDVVVLTDATRAVN
                     VRPDDGERALDALRAAGVREDRAEEAPLRRGA"
     gene            8115..>8453
                     /locus_tag="VEP73_06670"
     CDS             8115..>8453
                     /locus_tag="VEP73_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HYT26148.1"
                     /translation="MGRGAASDAERFREHWADEMDGAAMYRALAERASGEQREIFLEL
                     AGAEERHARHWAARLVALGEPEPRPGEHRRGLRTRLLSWLARRLGPSVVFPIIQRAEL
                     ADAGHYDEVPE"
BASE COUNT          977 a         3232 c         3173 g         1071 t
ORIGIN      
        1 cctggcgcca ccgctccgag cggtacgggg agaagctcgc gaccccggac accagcgtcg
       61 gcgacctcat cggcgacgtc gacccgatca aggtggccga gggccgcacc ctcggcgacc
      121 ccgagaccgt ccactacggg ctcgtgcccc gcaccaaccg gggcatcttc agcgtcaacg
      181 agctgcccga cctcgccgag cgcatccagg tggcgctcct caacgtgctc gaggagcgcg
      241 acatccaggt ccgcggctac cagctccggc tgcccctcga cctcctgctc gtggccagcg
      301 ccaaccccga ggactacacc aaccgggggc ggatcatcac cccgctgaag gaccggttcg
      361 gggccgaggt ccgcacccac tacccgctgc ggctcgacga cgagctcagg ctggtccgcc
      421 aggaggcggt gctgacctgg gagggcagcc cgctcgggaa gccggtgctc cccgaccacg
      481 tggtcgaggt catcgcccgg ttcacccgcg gggtgcgcga ggcgccgcag atcgacgcgc
      541 gctccggggt gtccgcccgc ttcgcgatcg cggcggccga gacggcggcg gcctccgcgg
      601 tgcgccgggc cgccgtgacc ggcgaggaga cccccgtcgt gcgggtcgtc gacctgcccg
      661 ccgtggtgcc cacctcgctc gggaaggtcg agttcgacca gctcgaggag ggcagggagc
      721 tcgacctgct caagcacctg ctccggcggg ccgtcgccga cacctaccgc agccggctcg
      781 gcgggcagga cgtctcgggc ctgctccggc gcttcgagga cggcgggacg gtcgagactg
      841 gcgacctcgt gccagcggcc gagctgctcc gccgcgtcgg gccggtgccc gggctggccc
      901 agctcctgcg gcgcctcggc gacgacgagg gcgccgagac ccccgggctg gccgcggccg
      961 ccctcgagtt caccatggaa ggcctccatc tcggcaagcg gctggccaag gaggaactcc
     1021 ccggccgggc cgtctacgga ggatgagcat tcgctacggg cgctggcggg ggggtcccga
     1081 cccgctcgag ccgcccttcg acgtggccgc cgccctcgac cgcatgggcg agcgggtgct
     1141 ggcgggggac cggacgggcg aggcgctgtc gtcgctcctg cgccagggca tgccgggact
     1201 gcggggcctg cgcgacctgc tccgccaggt ccgcgagcgc cgccgcgagg cgcgcgggcg
     1261 aggcgcgctg gacggcaccc tcgagcgcgt ccgcgagctg ctcgagcgtg cgctccggga
     1321 ggagcgggag gcgctcgccg ccgacccgtc ggacgccgcc cggctggcag gggccgagct
     1381 ggaccagctc cccggcgaca ccgccggggc cgtgcgccag ctcgccgacc gggaatggcg
     1441 ctcgcccgag gcggcggcca cctacgagga gatccgcgac ctgctccgcc gggaggtgct
     1501 cgacacccag ttccgcggca tgaagcaggc cctcatgggc gcgatgcccg aggacctcga
     1561 gcgggtgcgg gacatgctcg cggacctgaa cgcgatgctc gacgccgacg cgcggggcga
     1621 ggacacgacc gccgcgttcg accagttcat gcagcggtac ggcgacttct tcccggaggg
     1681 cccggagaac ctcgacgagc tggtcgactc gctcgcgcgc cgggccacgg cagccagccg
     1741 gctgctcggc tcgctctcgc cggagcagcg ggaggagctc tccgccctca tgcaggcggc
     1801 aatggccgac ctcgggctcg agcgcgagct cgaccgcctc ggccgggcgc tcgagtggcg
     1861 ccggcccgac ctccgctggg gccagggcgt gcccatgcgc ggcggccagc cgctcgggct
     1921 cggcgacgcc accaccgccc tcgaggagct ggccgacctg gaggcgctcg aggcgagcct
     1981 cggccaggac tacccgggcg cctccctcga cgacgtcgac ctggaggcgg tcgagcgggc
     2041 gctcggccgg ggcgcggccg ccgacgtcga gctgctccgc accctcgagc gcgagctggc
     2101 cgagcagggc tacctgaccc gcgagcacgg cgagctgcag ctcaccccga aggcgatccg
     2161 gcgcatcggc gccaccgccc tccggcggat cttctcgagc ctcgactcgc gcgcccgggg
     2221 cgaccacgac gtggccagcg cgggcacctc cggcgagccg accggcagct cccgtccctg
     2281 gaccttcggg gacgagcaac cgctggacgt ggtgggcacg gtgcggaacg cggtgctgcg
     2341 tggcggggcc cttcccgccg ggggggcggg aagcgccccc ccggaccccc ccggccgcct
     2401 ccttggcggt cgtggccctg caagggtggt gctcgaggcc ggcgacttcg aggtcctgga
     2461 cacggaacgt cgcacctcgg cggcgatctg cctgctggtc gacctgtcct actccatgct
     2521 gctgcgggag gcgtgggggg cggccaagtc gaccgcgctg gcgctgcaga gcctcgtgtc
     2581 cacccgctac ccgcaggacg cgctctcgat cgtcgggttc tcgtcggtgg cgaggaccct
     2641 gcggccgcgc gagctggccg acctcgcccc gaacatggac cagcagggca cgaacctaca
     2701 acatgcgctg ctgctcgccg gcaggcacct cgaccgccat cccgacgccg agccggtggt
     2761 cctggtggtc accgacggcg agccgaccgc ccatctgcag cccggcggcc acccctattt
     2821 cgagtggccg ccgagccagg agacgatcga gctgaccatg gccgaggtgc tgcggctgac
     2881 ccgcaggggc gccaccatca acgtgttcat gctcgacacc gagccgcgcc tcgtccgctt
     2941 cgtccaggcg ctggcaaggc tgaacggggg cagggtcttc accccgtcga gcgaccggct
     3001 cggcgactac gtggtgaacg actacctgcg cgcgcgccgg ggccgtcgcg ctcgctgacc
     3061 gccgggacgg cggaaggccc accggcggtg ccggtgggcc ttcgtggtac gagcggtgct
     3121 actgctcgag cggccgcacg ttcgccgcct gggcgccctt cggcccctgc gtgatgtcga
     3181 actcgacctg ctggccctcg ttgagcgagc ggtagcccga agcctggatc gcgctgaagt
     3241 gcacgaagac gtcctcgccc tgctcggggg cgatgaagcc gtagcccttc tcggcgttga
     3301 accacttcac ggtcccaaca gtcatgtacg ttgtccctcc cctccatcgc ttggaggtac
     3361 gatcaacgcc gacgcggatg ggacgcgccg acggcgaggg acgaactcga ccgcgggatg
     3421 cgaggcgaaa cgtgaacccc ttgcggccat gacgctagca tgcgcgcggc cattatgcta
     3481 accctggcgt tgagctgcgc cgttgcccgt caagagcgcg gcgtcgagca ggcggccctg
     3541 ctccccggcc gtggcgggct catctcgctc ccggcctcac cccaccaggc gggcctcggc
     3601 gtcggcgagc tcggcggccg tccgggcggc ccgctccctg gtcctccgct cggcctggac
     3661 ggcgtcgtcg gcggcctccc tggcgcggat ggccgcctgc tcggcgcggt cggcggcgtg
     3721 gcggagctgc tcggcctggc ggcgggcgag gatggcggcg tcggccgccg ccctggcctg
     3781 ccgccgcgcc tcgtcgcggg cgcgccggcc ggcgaggagc cgttcgttgc cctcggcgtc
     3841 ctcgacccgc ccggcttcga cccctcgccg gccggccggt cctgccgcct cggcctcggc
     3901 gacgccgaca ggccaggcgc cgccgccgaa gccgggcggg tcgaggtcgc gggccaggcg
     3961 gccctcgcgg gccagggccg ccgcgcgcgg ctcccgtgcg gcagcgtgca agctgcccgc
     4021 gatgcgctcg cgcacggcct ccgacgcggg ccggccggcg gcggccagct cgccggcggc
     4081 cgcctccacc agctcctgga cggcctcgcg ctcgtcgcgg gccgccttgg ccaggccgct
     4141 gcgcccggct ccggccagga cggcgtcgtg ggcggccctg agtcgctcgc cgcgctcgag
     4201 cagccggccg agccggctgg cgtgccgccg cgccagctgg ttcaccgccc acgcggacac
     4261 cgacggcttg cgcagcgcgg cgatgcgctg ggcggcggcg gcgtccccgg cctgcctgag
     4321 cgcgcgcgcc gcctcggccc tggcgttgac gaaggcgtcg agcggtagtc cgaacagccg
     4381 gtcgagttcc tgctcctccg tcatgcgctc ccacctcccg cggaacgggt gcccaccgcc
     4441 gtagcgtgcc aggtgacggc ggccggggga aggcgtctcg tcggccggga gccgccggcc
     4501 cgcgcccgcg gtgcttgccc cacgggaacg acgtgctaga atcacagcgc tgtaatttcc
     4561 cggccccggc cgggaaccgg gcgggccccg ggccgcgagg ggaagcgcgg cccggggcct
     4621 tgtctctccg cctgatcctg ctcggtccgg cccctgcccg gtccgggtcg tctgcccggt
     4681 ggtcgtctgc ccgtggtagg gtccgtacgt ggagatcgac ctgccggtgc accgccttga
     4741 gcccggcctg cccctgcctg cctacgccca cccgggggac gctggcgccg acctgttcgc
     4801 cgccgaggac gtgaagctgc cgcccggcgg tcgcgcggtc gtcgggaccg ggatggccgt
     4861 cgcgattccc gagggcttcg ccgccttcgt ccacccccgc tccgggctgg cggcgcggca
     4921 cggcgtcacc gtcgcgaacg ccccggggac ggtcgacgcc ggctaccggg gcgaggtcaa
     4981 ggtcgtcctg ctcaacaccg acccggccga gccccacgtc gtgcgccgcg gcgaccggat
     5041 cgcgcagctc gtggtccagc cggtggtgcg cgcacgcttc ctcgacgtac gggagctgcc
     5101 cccggccgcc cggggcggcg ggggattcgg ctccaccggc ccgtgaacat tccgcgaacg
     5161 ccgttttcga cccgccctcg ggcggaacac aaccggcagc atggatccac gtgacgggcg
     5221 cggcggcggc cgtgaccagc cctgggggga gcgcctggcc gacacggcgt cgtcgccggg
     5281 cggtgacccg accgccgggc gcctcatccg cgacgccatc ggcgtcgtcc gcgggcgcct
     5341 ctccgacgtc gcccgtggcg gcatccagcc cctgctggac gaggcggccg cccaggtgcg
     5401 cccggccacc ctcgacgagc tgcgcacccg ctacccgggc ctgaccgagg acgagatcgc
     5461 gcgcaagctg atcgaccgct ccgcccgcac cgccgtcggc gtcgcgctcg cgatcggcgg
     5521 gatcctggcc gcccaggaag ccgtcgcggt cgcctcgtcc ccggtgccgc cggccgccgg
     5581 cgcggccctc ggcaccgtcg gcgtcaccgc cctggccgag gtcctggtgc tgttcgtcat
     5641 cgaggccaag ctccgggccg acctgggtgc gctcgccggg cagcgcgtcc ccaccccccg
     5701 cgagctggtc gccggcgtgg tcggcgaggt ccaggcggcc ggcggcctgg gccggctgcg
     5761 caggcgctcg ctccgccgcg ccctgccgga ggcggcggtc ctgcgcgccg ccacccggat
     5821 cgcgaggctg atgccggccc gcttcgcccg tatcgtcatt ccagaggtgg tcgcgcccct
     5881 ggtcggggcc gcctgggccg cccggctcgc ctcccagcag ctcagggcga ccggcgagcg
     5941 ctgctggggc gagctgcggc tgctgccgag gaccacggtg agctggggcc cctcgacctg
     6001 gaacggcggg aacccttccg ggccgagggg cccgggcgac tcccgctcgc tccccccgcc
     6061 cgacccgacc tggcggccgc cgagctgacc cctcacggtc ggaggcacgc ggtcgggctc
     6121 agcgttcgat cggcacgttc acgagcgacc cccactcggt ccatgagccg tcgtagttgc
     6181 ggaccctcgg gtagccgagc agctcgtgga gcacgaacca ggtgtgggcg gagcgctcgc
     6241 cgatccggca gtaggcgacc acgtccttgt cgggcgtgac gcccttgccg ccgtacagct
     6301 cggcgagccg gtcgtcggtg aggaacctgc cggtgtcgcc gtcgaccgcc ttggcccagg
     6361 ggacgttcgc ggcgccgggg atgtggccgg gccgctgggc ggcctcctgc ggcaggtggt
     6421 cgggggcgag cttctcgccg cggtactcct cgggcgagcg cacgtcgacg agcgcggcgc
     6481 cgtcgcggcc cacgtagtcg ctgaggacct ggtcccggaa cgcgcggatc gacacgtcgg
     6541 gctcgcccgc cttgtagccg ctcgacggct cgacggccgg gctgtcggtc gtgagcgggc
     6601 ggtcctccag ctcccaggcc ttgcgcccgc cgtcgaccag cttgacgttc tcgtggccgt
     6661 agaccttgta gtaccagtag gcgtaggcgg cgaaccagtt gttgttgccg ccgtagagga
     6721 cgaccgtcgt gtcgttgccg atcccgaggg agtcctgcag cttcgcgaac gcctccgggg
     6781 agacgaagtc gcgccgcagc gggtcctgca ggtcggtgtt ccagttcacc gacaccgccc
     6841 cggggatgtg gccccggttg taggcgtcgg tgtcctcgtc cacctcgacg acgcggatgc
     6901 ccgggtcgtc caggtgctcg gcgagccagt cggtggtgac caggagctgg tcgcggtcgg
     6961 tcatggggtt cctcctctgg agggtgtgcg tctggagggt gcgcggcgga ggcggcgtcg
     7021 ctggcgcgga cggctcgcgg cgccggccgg gtgctggccg cggtgcgcgc gctacacgcg
     7081 aggacgcgcc gcggcctggc gacacggggg gcgcccggcg gccgagttgc cggtgccgag
     7141 ccccatcggt gcgcaggcgc gggtggtcag ggcggctggg ccccgctgcc gggccgcggc
     7201 cgccggcccg ggcgccggag gcacgtcggc cggaggcacg gagaggcgtg ccgaccggga
     7261 cagagagatc ggtgcgatca tgtgcttgcc aggctgccag tccctccccg tcgaagtcaa
     7321 ccagccggtc acgcccaggc cggccagccg gcgagccggt tgctaccctc ggtgtaccag
     7381 cgggcaccgg ccgcctgacg gccggtgccc gcttggccgg aaccgcgacc gcggaaggag
     7441 catccgatgg cgttcgaccc gacccccgac gacgccctgg tcatcatcga cgtgcagcgc
     7501 gacttctgcc cgggcggcag cctcgccgtc cctggcggcg acgaggtggt gccggtcgtc
     7561 aaccggctgg cgccccgctt cgcgacggtg gtgaccaccc acgacaccca cccggccgac
     7621 cactgctcct tcgcggcgcg gggcggccca tggccgcccc actgcgtcga gggcaccccc
     7681 ggctgggacc cccaccccgg cctggacgtg cgccccgact tccaggtgtt caaggggcgc
     7741 gaccgcgacc aggacggcta caccggctgg acgccggcgc tggccgactt cctctcccgg
     7801 cggggcgcgc gccgggtcgt cgcggcgggc ctcgccctcg actactgcgt gcaggccacg
     7861 gcgctcgacg ccagggccgc cgggttcgac gtggtggtgc tcaccgacgc cacgcgggcc
     7921 gtcaacgtcc ggcccgacga cggcgagcgg gcgctcgacg ctctgcgcgc tgccggcgtc
     7981 cgcgaggacc gggccgagga ggcgcccctg cgccggggcg cctgaccacc gccgcgcgcg
     8041 ctcgttgcgg ctgcggcgga atggtccaac actgctggtg gcagcgcggc gcgggagacg
     8101 tgggaggcag ggacgtggga cgaggagcgg cgtcggacgc agagcggttc cgggagcact
     8161 gggccgacga gatggacggc gccgcgatgt accgcgcgct ggccgagcgg gcctccgggg
     8221 agcagcgcga gatcttcctc gagctggccg gggccgagga gcgccacgcg cgccactggg
     8281 cggccaggct cgtcgcgctg ggggagccgg agccgcggcc gggcgagcac cgccgcggcc
     8341 tccgcacccg cctgctgtcc tggctggcgc gccgcctcgg ccccagcgtg gtgttcccga
     8401 tcatccagcg ggccgagctg gccgacgccg gccactacga cgaggtcccc gag
//
LOCUS       DASPYY010000428         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1055651, whole genome shotgun sequence.
ACCESSION   DASPYY010000428 DASPYY010000000
VERSION     DASPYY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055651"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1155)
                     /locus_tag="VEP73_06675"
     CDS             complement(1..1155)
                     /locus_tag="VEP73_06675"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYT26149.1"
                     /translation="MLDYLLRVSHELPPDELAGAVAAAAPLLGAGDVAVYLVDYEQVM
                     LMPLPDGSGRPPLEIDTTLAGRSFTASTVNEGDGDPGRRLWLPLLDGTDRLGVLALTL
                     DTPRVDDDLRARCVDLASVVAELLVSKGRYTDAFTLVRRRREMSVAAEMQWQLLPPLT
                     FLTPRVSIAGLLEPAYEVGGDAFDYALNGRVARLAIVDPVGHDLTASTLASVAIGGYR
                     HSRRAGLDLAATYEAVDRVLADRFGVERFVTGQLGELDCATGRLRWLNAGHPQPLLVR
                     HAKVVDALACDPSPPLGLGLGPAELATASLEPGDRVLFYTDGVVEGRDAAGEPFGDER
                     LVDVLARETLAGQGPAETMRRLSHAVLAHQDHRLRDDATMVFLEWPGARD"
     gene            complement(1204..1725)
                     /locus_tag="VEP73_06680"
     CDS             complement(1204..1725)
                     /locus_tag="VEP73_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26150.1"
                     /translation="MEDDRTTDHDDALVVMPLVVLSQTFPARPSAIPDIREFVRRYLA
                     ESPLSGEDTRELQRAVANALLDAASAVPASIQVSFRIFPDAVEIDVLRTPASPNAELP
                     VPQQARGEPGRPSFSSWMAAMLKREGLSQEAAARQLGVSVKTVSRWVGGETRPRQRER
                     QKLHDVFGEAPVP"
     gene            2018..>2211
                     /locus_tag="VEP73_06685"
     CDS             2018..>2211
                     /locus_tag="VEP73_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HYT26151.1"
                     /translation="MAQRLSERLHEAAVEAANRRSAPAKLLAQDADRVARACYDIAVR
                     FHRGGKLLVFGNGGASTDAQ"
BASE COUNT          308 a          750 c          870 g          283 t
ORIGIN      
        1 tcagtcccgc gcgcccggcc actcgaggaa gaccatggtg gcgtcgtcgc gcagcctgtg
       61 gtcctggtgg gccaggacgg cgtgggagag ccgccgcatc gtctcggccg gcccctggcc
      121 ggccagcgtc tcccgcgcga ggacgtcgac gaggcgctcg tccccgaacg gctcgccggc
      181 ggcgtcgcgg ccctcgacca ccccgtcggt gtagaacagc acgcggtcgc cgggctcgag
      241 ggacgccgtg gcgagctcgg cgggaccgag gccgagcccg aggggcgggg acgggtcgca
      301 ggccagcgcg tcgacgacct tggcgtggcg gacgagcagg ggctgcgggt gcccggcgtt
      361 cagccaccgc aggcggccgg tggcgcagtc gagctcgccg agctgcccgg tgacgaagcg
      421 ctcgaccccg aaccggtcgg ccagcacccg gtcgaccgcc tcgtaggtgg cggcgaggtc
      481 gagcccggcg cgccggctgt gccggtagcc gccgatcgcg acgctcgcca gcgtgctggc
      541 ggtcaggtcg tgaccgaccg ggtcgacgat cgcgaggcgg gcgacgcggc cgttgagcgc
      601 gtagtcgaag gcgtcgccac cgacctcgta ggcgggctcc agcaggccgg cgatggacac
      661 ccggggggtg aggaacgtga gcggcggcag cagctgccac tgcatctcgg cggcgacgct
      721 catctccctc cgccggcgga cgagcgtgaa ggcgtcggtg taccggccct tgctcacgag
      781 cagctcggcg acgaccgacg ccagatccac gcagcgggcc cggaggtcgt cgtccacccg
      841 gggtgtgtcc agggtgaggg cgagcacgcc caggcggtcg gtgccgtcga gcagcggcag
      901 ccacagccgc cgccccgggt ccccgtcgcc ctcgttgacg gtcgaggcgg tgaacgacct
      961 gccggcgagg gtggtgtcga tctcgagcgg cggccggccg ctgccgtcgg gcagcggcat
     1021 gagcatgacc tgctcgtagt cgaccaggta gaccgcgacg tcgccggcgc cgaggagcgg
     1081 ggcggcggcc gccaccgccc cggcgagctc gtcgggcggc agctcgtgcg acacgcgcag
     1141 caggtagtcg agcatccagc gagtatcccg ggccgggcgc cgcgagacaa ccacggccgc
     1201 cgctcaggga accggggcct cgccgaagac gtcgtggagc ttctggcgct cgcgctggcg
     1261 cgggcgggtc tcgccgccca cccagcggct gaccgtcttc accgagacgc cgagctggcg
     1321 cgctgccgcc tcctgggaca ggccctcccg cttgagcatc gccgccatcc aggaggagaa
     1381 cgacggtcgc ccgggctcgc cgcgcgcctg ctgcgggacc ggcagctcgg cgttggggga
     1441 ggcgggtgtg cgcaggacgt cgatctccac ggcgtcgggg aagatcctga aggacacctg
     1501 gatcgacgcc gggaccgcgc tggccgcgtc gaggagggcg ttcgcgaccg cccgctgcag
     1561 ctcccgggtg tcctcgccgc tcagcggcga ctcggcgagg tagcggcgga cgaactcgcg
     1621 gatgtccggg atcgccgagg gtcgtgccgg gaatgtttgg gacagcacga cgagcggcat
     1681 cacgacgagt gcgtcgtcgt gatcggtggt gcggtcgtcc tccaaacccg gagtcctccc
     1741 tgtgacgagg ggtgtggggt tgggcgtgct ggctctgttc gtccgagatt gtatgccagt
     1801 tcatgagcgg cagcccaggg gagtgtccca ccggttcagg gcgaagggga gaggccacaa
     1861 aggggggcac gtcgcatccg caacgtgtga gaatgtcggg gacatcgagg tcctgtccgg
     1921 acagccgtaa gactgtccgg acaatgtccg gacagtcttg cattggccat gacgggggag
     1981 catgctcggg gcgtccgggt cgcccgggag gttcgcaatg gcgcagcgcc tgtcggagag
     2041 gttgcacgag gcggcggtcg aggccgcgaa caggcggtcg gcgcccgcga agctgctcgc
     2101 ccaggacgcc gaccgggtcg ccagggcgtg ctacgacatc gcggtccgct tccaccgggg
     2161 cgggaagctc ctcgtgttcg gcaacggcgg cgccagcacg gacgcccagc a
//
LOCUS       DASPYY010000429         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2179791, whole genome shotgun sequence.
ACCESSION   DASPYY010000429 DASPYY010000000
VERSION     DASPYY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179791"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1083)
                     /locus_tag="VEP73_06690"
     CDS             complement(382..1083)
                     /locus_tag="VEP73_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYT26152.1"
                     /translation="MRERAGPTRRRGRPAVFLDRDGTLVEEVPYLHEPDRVALLPGVG
                     GALRALAAAGFALVVVTNQAGVARGHYGEAAVAAVHRRLAVLLRAEGVRLDGIWYCPH
                     HPDGTVAAYARACRCRKPAPGLLEAAAGALGLDLGRSWLVGNHPSDVAAGAAAGVTPL
                     FVSTGDGATRAPPPGTPALPDLAAAAGVVLGCPVVAAPPSGRPTGPPRWEEAGPWGGA
                     GAEHRPERRPGPPRP"
     gene            complement(1116..1691)
                     /locus_tag="VEP73_06695"
     CDS             complement(1116..1691)
                     /locus_tag="VEP73_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYT26153.1"
                     /translation="MTDVAGYLSMSAAAAAALVVRPGAGAIERAARLVADALTDGGQV
                     LFCGNGGSAADAQHLAAELVGRLARERPAYRAVALTTDSSVLTAVGNDYGYAEVFARQ
                     VQGLGRRGDVLVAVTTSGRSENVLRAAAVAREAGLAVVAFLGPGRSPLDDMADVALHV
                     DGESAGLIQQGHITIGHALCGWVEARLAGPR"
     gene            complement(1688..2689)
                     /locus_tag="VEP73_06700"
     CDS             complement(1688..2689)
                     /locus_tag="VEP73_06700"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HYT26154.1"
                     /translation="MTAVRASAPLRISFAGGGTDVPPYPSTHGGAVLSATISRCATAH
                     VQVRSDGAYHVDSLDLMARAAFTAREDLAFNGHLDLVKAVLRGLDPGGGLDLVLATDA
                     PPGSGLGSSSALVVAMLAAVGATAGRSWSPAELAWRAYEVERIDLKIQGGMQDQYAAA
                     FGGLNFIEFHGDDDVEVRPLEVRSSTVEELERSLLLAWSGQGRTDGGILRRQIDGVLG
                     GSARSLGSLGAMKALAEAMRDALLAGDLATFAEGLHEGWEQKRRLATGIATPRLEELY
                     EVGRAAGAAGGKVLGAGGGGFLVFCGPPAHGDRVAAALEAAGAPCSPVAFDPRGVRL"
     gene            complement(2686..>2904)
                     /locus_tag="VEP73_06705"
     CDS             complement(2686..>2904)
                     /locus_tag="VEP73_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26155.1"
                     /translation="SGGAPETVEEGVTGFVVDGRRPEPVAAAVAELLGDLPRARAMGA
                     AGRRRVEAEFSWDVVVAQFEKLLAQAAT"
BASE COUNT          357 a         1119 c         1131 g          297 t
ORIGIN      
        1 ggggatggtc ggcgccagcc gcgagaacgt cagccgcgcc ctggccgggt tccggcggcg
       61 cggcttcgtg gcctacgaca gcgacgcgat ccacctgctc gacctcgacg cgctccggca
      121 cctgacctga ggcgcccgac ctcggcaagg acccaggtca ccgcgggcct gtgacgcaga
      181 tcacagcgaa gctgtgacgc acgtcactga cccggggacg gcacaccaac tacggtgcgg
      241 gcgccttccc ccggggctcg gtcgaccgga tgcgccgagc ctggagaagg tggcagcggc
      301 aaggtcggcg aaccgggggg gccgctcgga cgaccgctgg tggtgccggt ctaccgccgg
      361 catcccacca ggcccgcctc gtcacggccg aggcgggcct ggtctccgtt ccggccggtg
      421 ctccgctccg gccccgcccc acgggcccgc ctcctcccac cgaggcgggc ccgttgggcg
      481 cccggacggt ggggccgcca ccacagggca cccgaggacc acgccggccg cggccgccag
      541 gtcgggcagg gcaggcgtgc ccggcggcgg cgcccgggtg gccccgtcgc ccgtggagac
      601 gaacaggggc gtgacgccgg cggcggcgcc cgccgccacg tcgctgggat ggttgccgac
      661 cagccaggat cgtccgaggt cgagaccgag ggcgccggcg gcggcctcga gcaggccagg
      721 ggccggcttg cggcagcggc aggcccgcgc gtaggcggcc accgtgccgt ccgggtggtg
      781 cgggcagtac cagatgccgt cgagccgcac cccctcggcg cgcagcagca cggccaggcg
      841 ccggtgcacg gcggcgaccg ccgcctcgcc gtagtggccg cgggccacgc cggcctggtt
      901 ggtgaccacc acgagcgcga acccggcggc ggcgagcgcc cggagcgccc cgcccacgcc
      961 gggcagcagg gcgacgcggt ccggctcgtg caggtacggg acctcctcga ccagggtgcc
     1021 gtcgcggtcg aggaacacgg cgggccgccc ccgccgccgg gtcggcccgg cgcgctcgcg
     1081 caccgcctgt ccggccgggg gcgggcccgc gcgggtcatc gcggcccggc caggcgggcc
     1141 tccacccatc cgcacagcgc gtgaccgatg gtgatgtgcc cctgctggat cagcccggcg
     1201 ctctcgccgt cgacgtgcag cgcgacgtcg gccatgtcgt cgagcggcga gcggcccggc
     1261 ccgaggaacg cgaccacggc gagaccggcc tcccgggcga cggccgccgc ccgcagcacg
     1321 ttctcggagc gcccgctcgt ggtcacggcg accagcacgt cgccgcgccg gccgagtccc
     1381 tggacctggc gtgcgaagac ctcggcgtag ccgtagtcgt tgcccaccgc ggtgaggacc
     1441 gagctgtcgg tggtgagcgc gaccgcgcgg taggcggggc gctcccgggc gaggcggccg
     1501 accagctcgg cggcgaggtg ctgggcgtcc gccgcgctgc cgccgttgcc gcagaacagc
     1561 acctgcccgc cgtcggtcag cgcgtcggcg accaggcggg ccgcccgctc gatggcgccg
     1621 gcgcccggcc ggaccacgag ggcggccgcg gcagcggccg acatggacag gtacccggcg
     1681 acgtcggtca aaggcgcact cctctcgggt cgaaggcaac cggcgagcag ggcgccccgg
     1741 ccgcctcgag cgcggccgcg acccggtcgc cgtgggcggg cgggccgcag aagacgagga
     1801 atccgccgcc cccggccccg agcaccttgc cgcccgcggc gccggcggcc cggccgacct
     1861 cgtagagctc ctccaggcgc ggggtcgcga tgccggtcgc caggcggcgc ttctgctccc
     1921 acccctcgtg gagtccctcg gcgaaggtgg cgaggtcgcc ggcgaggagc gcgtcccgca
     1981 tcgcctcggc gagggccttc atcgcgccga ggctgccgag gctgcgggcc gagccgccga
     2041 ggacgccgtc gatctggcgc cggaggatgc cgccgtcggt gcggccctgc cccgaccagg
     2101 cgagcagcag ggagcgctcg agctcctcga ccgtcgacga gcggacctcg agggggcgca
     2161 cctcgacgtc gtcgtcgccg tggaactcga tgaagttgag cccgccgaac gcggcggcgt
     2221 actggtcctg catgccgccc tggatcttga ggtcgatgcg ctcgacctcg tacgcccgcc
     2281 aggccagctc ggcgggggac caggagcgac cggcggtggc cccgaccgcg gcgagcatcg
     2341 cgaccacgag cgccgaggac gacccgagcc ccgagccggg gggcgcgtcg gtggccagca
     2401 cgaggtcgag gccgccaccc gggtcgaggc cgcgcaggac cgccttgacg aggtcgaggt
     2461 gcccgttgaa ggcgaggtcc tcgcgggccg tgaacgcggc cctggccatc aggtcgaggg
     2521 agtcgacgtg gtaggcgccg tcggagcgga cctgcacgtg cgcggtggcg cagcggctga
     2581 tggtggcgga gagcaccgcc ccgccgtgcg tggacgggta gggcgggacg tcggtccctc
     2641 ccccggcgaa gctgatccgc agcggcgcgc tcgcccggac ggcggtcatg tcgccgcctg
     2701 cgcgagcagc ttctcgaact gggccacgac cacgtcccag gagaactccg cctcgacccg
     2761 gcgccgcccg gccgcgccca tcgcgcgggc ccgcggcagg tcgccgagca gctcggcgac
     2821 ggcggcggcg accggttcgg gccggcgccc gtcgaccacg aagccggtca ccccctcctc
     2881 gacggtctcg ggcgcaccgc ccga
//
LOCUS       DASPYY010000430         1859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_71943, whole genome shotgun sequence.
ACCESSION   DASPYY010000430 DASPYY010000000
VERSION     DASPYY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71943"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VEP73_06710"
     CDS             <1..1112
                     /locus_tag="VEP73_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT26156.1"
                     /translation="ALARRLHLPVVTTMPGRGVLSDDHPHLVGAAGSSGHRSAHRTLE
                     RCDTLLALGVSTRGGAFDIPGGAFLIQVDRDPLQLCRRTHRGVGLYGTVAETLDELLA
                     RLAPPTVVSPGRSRGGGREASRRRFLRARQASFGRWRRRTRRTGRVPRAGRPIPPSLL
                     CRTLREVLAGLGDEGEAMGRPVVTVDVGLTTLWVYRYLPGQVDFVWTSSFAAMGFAMP
                     AALAIAPLAPGRPVLALVGDGGIGITMAELAAATHAPAPVVVVVFNNGKLAAIKYEQE
                     VMGWPEHESELCNGDFASYARACGVNGVRVTNPEQLAPALRAALAGRTTCLLDVVCDP
                     HETPWPARPSPRQAAGFLLARVREARASIRHWRRA"
     gene            1109..>1859
                     /locus_tag="VEP73_06715"
     CDS             1109..>1859
                     /locus_tag="VEP73_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYT26157.1"
                     /translation="MTRARSGFGTGAGMPPVEIDLATGQCRRFGPSPWLRHALEQASR
                     LGRLDGTGLYYPPGGDPELCALVLELQDDHVALRSRDTAVLITSGAINALDALLRAHL
                     APGDEVLVPDPGFPPYHSVVRLAGGRPVPYALRPTGPGFALDLDDLAGKLSARTRFLI
                     LNSPHNPTGHVLGEGELRGVAELLDAHPQVAYISDEVYARLVYEGASARSIAALSPAG
                     VVIDSLSKSHGLAGYRVGWLYGDPGLVDGARA"
BASE COUNT          219 a          736 c          657 g          247 t
ORIGIN      
        1 aggcgctcgc ccggcggctg cacctcccgg tcgtcaccac catgcccggc aggggggtgc
       61 tctccgacga ccatccgcac ctggtcggcg cggcgggctc gtcagggcat cgcagcgccc
      121 accgcaccct ggagcgctgc gacacgctgc tcgcgctcgg cgtgtccacg cgcggcggcg
      181 ccttcgacat ccccggcggc gccttcctca tccaggtcga ccgcgacccg ctgcagctgt
      241 gccggcggac ccatcggggc gtcgggctct acggcaccgt cgccgagacg ctcgacgagc
      301 tgctggcccg gctcgccccg ccgaccgtcg tcagccccgg gcgctcgcgg ggaggtggcc
      361 gcgaggccag ccggcggcgg tttctgcggg cgcggcaggc gagcttcggc cgctggcggc
      421 ggcggacgag gcgcacgggc cgcgtgccgc gcgccggccg ccccatcccg ccgtcgcttc
      481 tctgccgcac cctgcgcgag gtcctcgccg ggctcggcga cgaaggggaa gcgatggggc
      541 ggccggtggt caccgtcgac gtcggcctca ccaccctgtg ggtataccgc tatctgcccg
      601 gccaggtaga cttcgtctgg accagctcgt ttgccgcaat ggggttcgcc atgccggcag
      661 cgctcgcgat cgctccgctg gcgccaggcc ggccggtgct cgccctggtc ggcgacggcg
      721 gcatcggcat caccatggcc gaactggccg ccgccaccca cgccccggcc ccggtcgtgg
      781 tggtcgtctt caacaacggc aagctcgccg ccatcaagta tgagcaggag gtgatgggct
      841 ggcccgagca cgagtcggag ctgtgcaacg gcgacttcgc cagctacgcc cgcgcctgcg
      901 gcgtcaacgg ggtgcgggtc accaacccgg agcagctcgc gccagcgctg cgcgccgcac
      961 tcgccgggcg cacgacctgc ctgctcgacg tggtttgcga cccgcacgag acgccctggc
     1021 ccgcccggcc cagcccgcgg caggcggcag gcttcctgct cgcccgggtg cgggaagccc
     1081 gagcctcgat caggcactgg aggcgggcat gacccgggcc aggtccgggt tcgggaccgg
     1141 ggccgggatg ccgccggtcg agatcgacct ggctaccggg cagtgccgcc gcttcggccc
     1201 ctcgccgtgg ctccgccatg ccctcgaaca ggcatcccgg ctcggccggc tcgacgggac
     1261 cggcctgtac tacccgccgg gcggcgaccc ggagctgtgc gcgctggtcc tcgagctgca
     1321 ggacgaccac gtcgccctgc ggtcgcgcga caccgcggtg ctgatcacca gcggcgcgat
     1381 caacgccctc gacgccctgc tgcgggcgca tctcgctccc ggcgacgagg tcctcgtccc
     1441 cgatcccggc ttcccgccgt accacagcgt ggtccggctc gccggcggca ggcccgtgcc
     1501 atacgcgctg cgccccaccg ggccgggctt cgcgctcgac ctggacgacc tagccggcaa
     1561 gctcagcgcc cggacccgct tcctgatcct caactcaccc cacaacccga ccgggcacgt
     1621 gctcggcgag ggcgagctgc gcggcgtggc cgaactgctc gacgctcacc cacaggtggc
     1681 gtacatctcc gacgaggtct acgcgcggct ggtctacgag ggcgcgtccg cgcggtccat
     1741 cgccgcgctg tcgcccgccg gcgtggtgat cgacagcctc tccaagtccc acgggctcgc
     1801 cggctaccgg gtcggctggc tgtacggcga ccccggcctg gtggacggag cccgggccg
//
LOCUS       DASPYY010000431         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_916094, whole genome shotgun sequence.
ACCESSION   DASPYY010000431 DASPYY010000000
VERSION     DASPYY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916094"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            861..2069
                     /locus_tag="VEP73_06720"
     CDS             861..2069
                     /locus_tag="VEP73_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26158.1"
                     /translation="MSDLVSDRKANPDGQWYLDHAGPEAEDGGAYARALYGDGHRDDL
                     HAGPGTGLGYTDSAYARALYRSAPPDPDVTDPNLTDSAYDRALYAEPARPYDQGERGA
                     DLAQPGYGTPYGDPDATGPFYGDHDVDLAYLGHADPAAAGGGYRHDDGTAYRREQEPF
                     EDDPAQELEDELVDPGSLPVRRLRRLDSGRAATRRGTSLVNGVTMTLAAGIGVASLAA
                     VMAALTGHMLTTRQLFFMTHAACGIVIVHAFGGGLTTLAMTKESRTKELVRKWSVAVM
                     AVVAWITVLVGTWLGYPGYRAEPAHGAVNLNAYPRAFLLAHRDLAFWDTFAMEWKVNS
                     GWVTPFLATAVAFVVLRHHRLLLNDPRLRRMLTNLFVIAFAAALLGSMLGAVVNVVAP
                     NDFLHRPWHP"
     gene            2161..2727
                     /locus_tag="VEP73_06725"
     CDS             2161..2727
                     /locus_tag="VEP73_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26159.1"
                     /translation="MNELTRRRFEATALEFLGPVYDYAYRLTSDSSEAQDLTQETFLR
                     AFRSFDSFQPGTNCRAWLFRIAYNLSCNDYRRRLRMPRVDQVDKHADLFADLASDAPG
                     PEEQVLRLLDGEALHRALAELPDRFRDAVILVEIHGLSCAEAGEVMGTPRGTVLSRLH
                     RARGRLRQLLAVQAQDRAVEAGECAKAS"
     gene            2759..3661
                     /locus_tag="VEP73_06730"
     CDS             2759..3661
                     /locus_tag="VEP73_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYT26160.1"
                     /translation="MKKLILATLVAAAAAILVASVAPGRYGAGNYIQSAVDGGSAAPR
                     PAAGAAPASSAGAASSGAAMAPLQPSRAAPAPQQNAAIAKTAPTYTSGTGQGGYGGGS
                     GGTGSGGSSRPGGKGDARSRAAGDGWTTLAATLTGAAERPGPGAPGASGQVTVRLKGT
                     QVCFQESWQGIEATASHIHRGDRNAAGPVVVPFFKSSTPVSVQSATGCVTAAAALVRD
                     ISQHPGSFYVNVHTVELPAGALRGQLAMAAGPGGLAMTGTRSQATLVAGLAVAAAGLL
                     LVVSARGRPGRRVAVAGRHAARNR"
BASE COUNT          535 a         1438 c         1273 g          471 t
ORIGIN      
        1 ccgccggacc gctctgcgga cgcggcgcgt gccccggcgg gaggccagcc gcgacgcccg
       61 agcctccgcg tggaggtccg caatgtgctc ccgagccatg acatccagga acagtgggct
      121 catgtcgcct tccttccatc caagtccgtc accgtcatgt ctattatacc agtgacgtca
      181 ccatcgatgc cagcttggga gtgatgaaaa gcgccgcgtc gtccctgctc atcacggtcc
      241 cggtaagggc ggcggtgacg agctgagctt gcaccaccat acccgcgggg tgtgacattc
      301 ccgaccgccc ggctccagcc ggtcgccggc acggaggcct gacccgccgc cagaggccgt
      361 ctcgcaagat acgcgggcgt gtcacgggac gatgacggtc cgtgttcgat ttgactgcga
      421 tgtgcccgtg ccgtaagttc cgcccgagca accccccaag ctctccactc ggacggccag
      481 cccccactgc gccatccacc cggtgccctg acccttcagg ccaccggcat gcccccaagc
      541 cccatacgca atcccccaag aggctcgctg cagcttcgat gccggaccgg tccgatgcat
      601 cggtcggtcc atggcggcgc cacggccccc acctcggcgc tgccgcctcc ccggcggtcg
      661 cctggcatcg gcgtgaaggc ggccagcccc ctcctgcctc gaccgaggtc ctcgatgacg
      721 cgcgtgcccg tggagacgac agcggcgctg gcggccgccg gccaccgaac gacacccaac
      781 cgaccgcccg gcgacgggcg ctcggccgcc gtgccctgga ccgacccggt ccgggggccc
      841 gtgacgtcga tcgagagagg gtgagcgacc tcgtgagcga caggaaggcg aacccggacg
      901 gccagtggta cctggaccat gctggaccgg aggcggagga cggcggcgcg tacgcgcgcg
      961 ccctctacgg ggacgggcac cgcgacgacc tgcacgccgg gcccgggacc gggctcggct
     1021 acacggactc ggcctacgcc cgagccctct accgcagcgc cccccctgat cccgacgtca
     1081 ccgacccgaa cctgaccgac tcggcgtacg accgcgcgct ctacgccgaa ccggcgcgcc
     1141 cctacgacca gggcgagcgc ggtgccgacc tcgcccagcc cggctacggc accccgtacg
     1201 gcgacccgga cgcgaccggc ccgttctacg gcgaccacga cgtcgacctc gcctacctgg
     1261 gccacgcgga ccccgcggcg gcgggcgggg gctaccggca cgacgacggg accgcctatc
     1321 ggcgggagca ggagcccttc gaggacgacc ccgcccagga gctcgaggac gagctggtcg
     1381 accccgggtc gttgccagtg cgacgcctgc ggcggctgga ctcgggccgc gccgccaccc
     1441 ggcgggggac gtcgctcgtc aacggcgtca cgatgaccct cgccgccggc atcggcgtcg
     1501 cgtcgctcgc cgcggtcatg gccgcgctca ccgggcacat gctgaccacc aggcagctct
     1561 tcttcatgac ccacgccgcc tgcggcatcg tcatcgtcca cgccttcggc ggcggtctca
     1621 cgacgctggc catgaccaag gagtcccgga ccaaggagct cgtccggaag tggagcgtcg
     1681 cggtcatggc cgtcgtcgcg tggatcacgg tgctggtcgg gacgtggctg ggctacccgg
     1741 gctaccgggc cgagcccgcc cacggggccg tcaacctcaa cgcctacccg cgggccttcc
     1801 tcctggccca cagggacctg gccttctggg acaccttcgc catggagtgg aaggtgaact
     1861 cgggctgggt cacccccttc ctcgcgaccg ccgtcgcgtt cgtggtgctg cgccaccacc
     1921 ggctgctgct gaacgacccc cgcctgcgca ggatgctcac caacctgttc gtcatcgcgt
     1981 tcgcggcggc actcctcggc tcgatgctcg gcgcggtcgt caacgtcgtc gccccgaacg
     2041 acttcctgca ccggccctgg cacccatgag cggacggcaa gggcggatgc gccgctcccc
     2101 agcgatccca tggcgaggtg gaggcgagcc gtgccgaacg ggacagggac agatgacgcg
     2161 atgaacgaac tcacacgcag gcggttcgag gcgacggcgc tcgagttcct gggccccgtc
     2221 tacgactacg cctaccggct gacgagcgac tcgtccgagg cccaggacct cacccaggag
     2281 accttcctgc gggctttccg gtccttcgac tccttccagc ctggcaccaa ctgccgcgcc
     2341 tggctgttcc gcatcgccta caacctgtcc tgcaacgact accgccgccg cctgcgcatg
     2401 ccaagggtcg accaggtcga caagcacgcc gacctcttcg ccgacctggc cagcgacgcg
     2461 cccggtcccg aggagcaggt tcttcgcctc ctcgacggtg aggccctcca ccgcgcgctc
     2521 gcggagcttc ccgaccggtt cagggatgcg gtgatcctgg tcgagatcca cgggctctcc
     2581 tgcgcggagg cgggagaggt catgggcacg ccccgaggca cggtcctctc caggctgcac
     2641 cgcgccaggg ggcgcctccg ccagctcctg gccgtccagg cgcaggaccg ggcggtggag
     2701 gcgggggagt gcgccaaggc gtcatgacgt caccccaacc tgaaaggggc ctaccgtgat
     2761 gaagaagctc atcctggcca ccctggtcgc agcagcggca gccatcctgg tcgcgtccgt
     2821 cgcgccgggg cgctacggcg caggcaacta catccagagc gccgtcgacg gcgggtccgc
     2881 cgccccgcgg ccggccgcgg gcgccgcgcc agcgtcgagc gcgggcgccg cgtcctccgg
     2941 cgccgccatg gcgccgctcc agccgtcgcg agcggccccg gcgccacagc agaacgccgc
     3001 catcgcgaag accgccccga cgtacacgtc aggcaccggc cagggcgggt acggcggcgg
     3061 ctcgggcggc accgggtcgg gcggcagcag ccgaccgggc ggcaagggcg acgccaggag
     3121 ccgcgccgcc ggggacgggt ggaccaccct cgccgccacc ctcaccggcg cggccgagcg
     3181 gcccggaccg ggcgcgcccg gggccagcgg ccaggtcacc gtgcggctca agggcaccca
     3241 ggtgtgcttc caggagagct ggcagggcat cgaggcgacc gcctcgcaca tccaccgggg
     3301 cgacaggaac gccgccggcc cggtcgtcgt gccgttcttc aagtcgagca ccccggtctc
     3361 ggtgcagtcc gcgacggggt gcgtcaccgc cgccgcggcg ctcgtaaggg acatcagcca
     3421 gcaccccggc tccttctacg tgaacgtcca caccgtcgag ctgccggcgg gcgcgctcag
     3481 ggggcagctg gccatggccg ccggtcccgg gggcctcgcc atgaccggta ccaggtcgca
     3541 ggccaccctc gtggccggcc tggccgtcgc ggccgccgga ctcctgctgg tggtctcggc
     3601 ccgcgggcgt ccagggcgga gggtggccgt cgcgggccgg cacgcggcca ggaaccggta
     3661 gccggagacc ggaggacgtc ccggatgaac gcccgcccga gtgggtccgg cggtcgt
//
LOCUS       DASPYY010000432         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1758861, whole genome shotgun sequence.
ACCESSION   DASPYY010000432 DASPYY010000000
VERSION     DASPYY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758861"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1525)
                     /locus_tag="VEP73_06735"
     CDS             complement(<1..>1525)
                     /locus_tag="VEP73_06735"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HYT26161.1"
                     /translation="LHAWIEHSHRLVASVVIVLVGLLAVAAWRTGQARSVRWASAAAV
                     TMVLAQALLGAIVVWRKLEADSVTLHLATALALLALVEFIGFRSRWPVAAGRGEDPGE
                     ARSRRFVRLAAAAAALVYVQMLVGSTVTGHEAGLAFPDFPLMGGRWVPDLTLTAARLQ
                     VLHRALAVLVGAAVVGVWLRARRTQRAHPTVTRLAGYASGLVAVQIALGIANVWDRLS
                     ALTVVPHLAVGSLLWGTMFLLVLHADRFAGTGRRDPAEPEPSPRRGARQAVGAYFLLT
                     KPRIIELLLVTTIPAMFIARRGVPPAWLVAATLVGGSLSAASANTINCYLDRDIDQVM
                     HRTSRRPLPARQVEPADALRFGVVLGLAGFVWLWATVNLLSAALATAAILFYVFVYTI
                     GLKRRSTQNIVIGGAAGAVPVLVGWSAVTGTVGIPALVLFAIIFYWTPPHFWALSLKY
                     REDYAAAGVPMLPVVRGPRETSHQILYYTLLLVSVTLLLYPAGRMGIVYLVAAVVLGA
                     "
BASE COUNT          245 a          552 c          581 g          147 t
ORIGIN      
        1 ggcgccgagc acgacggcgg ccaccaggta gacgatgccc atgcgcccgg ccgggtacag
       61 cagcagcgtg accgacacca gcagcagggt gtagtagagg atctggtggg acgtctcccg
      121 ggggccgcgc accaccggca gcatcggcac cccggcggcc gcgtagtcct cccggtactt
      181 gagcgacagc gcccagaagt ggggcggggt ccagtagaag atgatggcga acaggacgag
      241 cgcggggatc ccgacggtgc cggtgaccgc cgaccagccg accagcaccg ggaccgcccc
      301 ggcggccccg ccgatgacga tgttctgggt cgagcgccgc ttcagcccga tcgtgtacac
      361 gaacacgtag aacaggatcg ccgccgtcgc cagcgccgca ctgagcaggt tcaccgtcgc
      421 ccacagccac acgaaccccg cgaggccgag cacgacgccg aaccgcaggg cgtcggccgg
      481 ctcgacctgg cgggccggca gcggccggcg cgaggtgcgg tgcatcacct ggtcgatgtc
      541 gcggtcgagg tagcagttga tggtgttggc gctcgcggcc gacagcgagc cgccgaccag
      601 cgtcgccgcg accagccagg ccggcggcac gccgcgccgg gcgatgaaca tcgccgggat
      661 cgtggtcacg agcagcagct cgatgatgcg cggcttggtc agcaggaagt aggcgcccac
      721 cgcctgcctc gcgccacggc ggggggacgg ctccggctcg gccgggtcgc gccgcccggt
      781 gccggcgaac cggtcggcgt gcagcacgag caggaacatc gtgccccaca ggaggctgcc
      841 gacggccagg tgggggacga cggtcagggc agagagccgg tcccagacgt tcgcgatccc
      901 gagggcgatc tggacggcca cgagcccgct cgcgtacccg gcgagccggg tgaccgtggg
      961 gtgcgcccgc tgggtccgcc gggcgcgcag ccagaccccg acgaccgccg cgccgaccag
     1021 cacagccagc gcgcggtgga gcacctgcag cctggcggcg gtgagggtga ggtcgggcac
     1081 ccagcgcccg cccatgagcg ggaagtcggg gaaggcgagc ccggcctcgt ggccggtcac
     1141 cgtcgacccg acgagcatct gcacgtagac gagcgccgcc gccgcggcgg cgaggcgcac
     1201 gaaccggcgc gacctggcct cgcccgggtc ctccccgcgg ccggccgcca ccggccagcg
     1261 ggagcggaac ccgatgaact ccaccagcgc gagcagcgcg agcgcggtcg cgaggtgcag
     1321 ggtgaccgag tcggcctcga gcttgcgcca gacgacgatc gcgccgagca gcgcctgggc
     1381 gagcaccatc gtgacggccg cggcgctcgc ccagcggacg ctcctggcct gcccggtgcg
     1441 ccaggcggcg accgcgagga gcccgaccag cacgatgacc accgacgcga ccagccggtg
     1501 ggagtgctcg atccaggcgt ggagc
//
LOCUS       DASPYY010000433         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1618529, whole genome shotgun sequence.
ACCESSION   DASPYY010000433 DASPYY010000000
VERSION     DASPYY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618529"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VEP73_06740"
     CDS             complement(<1..667)
                     /locus_tag="VEP73_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HYT26162.1"
                     /translation="MTRIGVLTGGGDVPGLNPCIKTLVYGAVDRGYEVVGLRRGWAGL
                     LDYDLDAPVPQDHCAQPLTRNAVRTIDRTGGTFLHTSRTNPGRVKPRDVPEFLKGQGQ
                     GESDGPFDFTHHALAVIDRLGLDWLVAIGGDDTLSYAERLSREGVRVVAVPKTMDNDV
                     HGTDYCIGFSTAVTRSVQFIHQLRTSTGSHERIAVIELFGRNCGETSLLSAYLAGVDR
                     AIIS"
     gene            853..1644
                     /locus_tag="VEP73_06745"
     CDS             853..1644
                     /locus_tag="VEP73_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT26163.1"
                     /translation="MAAGRPRAFDAHLHVIDPRFPLVPNRGYVPGPFTVDDYRARTAT
                     LGVVGGTVVSGSFQAFDQAYLLDALERLGPGFVGVTQLPATVTDEQVAELDARGVRAV
                     RFNLYRGGSEGLDQLEHLGRRVADLAGWHVELYVDARDLPELAPRLVRLPRLSVDHLG
                     LSRAGLPALLALVERGARVKASGFSRGDLDVPAALRAIDAASPDALMFGTDLPSTRAP
                     RPFADEDLELVLDTLGDDHGRRALHDNAAAFYLASPPRGRRPPSG"
     gene            complement(1710..2654)
                     /locus_tag="VEP73_06750"
     CDS             complement(1710..2654)
                     /locus_tag="VEP73_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYT26164.1"
                     /translation="MLDVRRLKVLREVARRGSFTAAARALAYTPSAVSQQIAALEREA
                     GAILVVRGPQGVSLTEPGRVLAAGADEILGRLAAAEGEIQAIAGLRAGLLRLGWFATA
                     GATLMPRAIAAFQRQYPDVELDLFEGDPDECIPKLRARELELALVYEFELAEPMGTDL
                     EQAPLVHDPLHVGLPPGHPLAHRARVRLADLAGDRWIQGVRHGSTVEELPRACRLVGF
                     EPHIAFQTDDRIAVEGLVAAGVGVALIPRLTLPTVRRDVAVRPLDARGLSREVRAALP
                     LGSYRPPAATAMLRILHEVCDGIVADAADRLHQRDAEG"
     gene            2758..4062
                     /locus_tag="VEP73_06755"
     CDS             2758..4062
                     /locus_tag="VEP73_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYT26165.1"
                     /translation="MAEIDVHGPRGAGVERRLERVLGLREAVALGVGGTVGGGVYVLI
                     GAAAGEAGPAAVAAFALAFLAAALIALPYAELACRYPLAGGGYAFARAVFGPHWGFLM
                     GWGYWGAYVFVSGYVTVGFGGYLAAVTGIPPVAGALGLIAACLGLNLLGVRASGRTQA
                     AVVGVALLGLVGFGVWGLPSVRAARFHPFLPHGPRGLLPASLLAFLAFGGFDMVAAAG
                     EEIDRPERNLPRAILLTLAAVLGLYLLVSLVAVGVVPSHALGASPAPLADAAATFGGA
                     PARRLLVLTALLTTAATANAVLVVTSRISFAMARDGLLPRALAAVGSRAGTPWASLVA
                     NALLLGAVALTGSVAFAAAAGGFLYVLHFVPPLVALVVLRRRHDAAPAFRTPLPAVVL
                     PLAFAAAAVLVAASGTTGAAAGLGWLAAGLAAHRLSPRPPVT"
     gene            complement(4125..4448)
                     /locus_tag="VEP73_06760"
     CDS             complement(4125..4448)
                     /locus_tag="VEP73_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26166.1"
                     /translation="MHFGIAVSVLVPWTETNRTHRFQIRIEYEDGGPPVVQMDGEVEV
                     GRPPGAQHGADQRAVLAINTLTQFPKAGGYRLVARANNSKVRTVSFRVRNQQIPFLPP
                     AQAAS"
     gene            complement(5076..6740)
                     /locus_tag="VEP73_06765"
     CDS             complement(5076..6740)
                     /locus_tag="VEP73_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HYT26167.1"
                     /translation="MRDYRTHHPGTYEEMTSSFRFELPAAYNIAADVLDKHEPDRPAM
                     RFVSDDGRDERWTFGDVRRLTDRAANLLRGLGVGKGDRVAVMAPASPEIAATFLATYK
                     CEAILLSLSVLYGDEGVVHRLNDSQAKVMVTSAEHRDRVAAMVEQAPSVERVVVVGGT
                     GGESFEELTRDASPEFATPATDPDTPAQLYYTSGTTGLAKGILHAHRYVLSHEEFQFC
                     HDVRAGELFHSTGEWAWIAGIVPGILGPWRFGVETLVHGRRGGFDPGKALRLIADHGV
                     GNLFTTPTAIRAMMRVDGAERLDFALRLACSAGEPLNPEAIAWWARAIGCPVLDYYGL
                     SESYPLCGNFPTVEVRPGSMGLPMPGWDVAVLDADEQPVPRGEAGEICLRARSNPHYP
                     LGYWNRPDDSEEVFGGTWFHTKDTAVQDEDGYVWYSGRADDVIIASGYRIGPFEVESV
                     LLEHPTVAESAVVASPDAQRGNVVKAFVRLAGGHEPGDGLVKELQDHVRTRLSAYAYP
                     RRVEFVDDLPKTLTGKIRRAELRQAEQQAARRAGGAAAGDASAEAT"
     gene            6827..7264
                     /locus_tag="VEP73_06770"
     CDS             6827..7264
                     /locus_tag="VEP73_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYT26168.1"
                     /translation="MYETRWPRPGPAMLAVGVVAGLVVGGILGISFPASSSDRHAAAP
                     TTSQVTQSSAQLPRSFFTLILASPSTSADAQSKLARYQAEGVADAVILSRASYPDLRT
                     QFAVCAGTYPSRTRADQALQELKAQLPNLTSSPYVRPMRRTTG"
     gene            complement(7592..>7836)
                     /locus_tag="VEP73_06775"
     CDS             complement(7592..>7836)
                     /locus_tag="VEP73_06775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26169.1"
                     /translation="GLLVPFEGSGGAPRDPERFAADFAERVNRLLADPDQAERFGKAG
                     RRRALDEFSWPAIAGQTARLYEELVSGAPPAGEAAG"
BASE COUNT          968 a         2895 c         2867 g         1106 t
ORIGIN      
        1 cggagatgat cgcccggtcc accccggcga gatacgccga cagcagcgag gtctccccgc
       61 agttgcgccc gaacagctcg atgacggcga tgcgctcgtg ggagccggtc gaggtccgca
      121 gctggtggat gaactggacg ctgcgggtca ccgcggtcga gaagccgatg cagtagtcgg
      181 tcccgtgcac gtcgttgtcc atcgtcttcg gcacggcgac gacccggacg ccctcgcgcg
      241 acaggcgctc cgcgtacgac agggtgtcgt cgccgccgat cgccaccagc cagtcgagcc
      301 cgagccggtc gatcacggca agggcgtggt gggtgaagtc gaacgggccg tcggactctc
      361 cctggccctg acccttgagg aactcgggca cgtcgcgggg cttgacccgc cccgggttgg
      421 tacggctggt gtgcaggaac gtgccgccgg tgcggtcgat ggtgcgcacg gcgttcctcg
      481 tgagcggctg cgcgcagtgg tcctggggca ccggcgcatc caggtcgtag tcgagcaggc
      541 cggcccaccc gcggcgcagc ccgacgacct cgtagccgcg gtcgaccgcg ccgtagacga
      601 gcgtcttgat gcacgggttc agcccgggga cgtcaccgcc gccggtgagc acgccgatcc
      661 tggtcatgtg gacggccctc ctgaagtctt cgcacgaaca tccggtccag gcggcgtcgc
      721 ccgggaaccc cattcgacat ccaaccccgc cgacccggca actttccgca ccgccacgag
      781 gcggtgcggc ggcgagcctc cggtgtacgg tctcggtccg ccgctcgagg tgccggaagg
      841 gggtcggagg acgtggccgc tggccggccg agggcgttcg acgcccacct gcacgtcatc
      901 gacccgcgct tcccgctcgt ccccaaccgg ggctacgtcc cggggccgtt caccgtcgac
      961 gactaccggg cccgtaccgc gaccctcggc gtcgtgggcg ggaccgtcgt gtccggctcg
     1021 ttccaggcgt tcgaccaggc gtacctcctc gacgccctgg agcggctggg gccgggcttc
     1081 gttggggtca cccagctgcc cgcgaccgtc accgacgagc aggtcgccga gctcgacgcc
     1141 cggggggtgc gcgcggtgcg gttcaacctc taccgcggcg gctcggaggg cctcgaccag
     1201 ctggagcatc tcggccgccg tgtggccgac ctcgccggct ggcacgtcga gctctacgtc
     1261 gacgctcgcg acctgcccga gctggcccca cgactcgtcc ggctccccag gctcagcgtc
     1321 gaccacctcg ggctgtcccg cgccggcctg cccgcgctgc tcgccctcgt ggagcgaggc
     1381 gcccgggtca aggccagcgg cttcagccgg ggcgacctcg acgtcccggc ggcgctgcgg
     1441 gcgatcgacg cggccagccc ggacgcgctc atgttcggca cggacctgcc ctcgacccgc
     1501 gcgccacggc cgttcgccga cgaggacctc gagctcgtcc tcgacaccct gggcgacgac
     1561 cacgggcgcc gggcgctcca cgacaacgcg gccgccttct acctggcgag cccgccgcgc
     1621 ggccggcggc cgccctcggg ctgacccgga cctgctcgga agccccgggg gctaccgctt
     1681 cgctgctcga gcccccggac cccgctggct cagccctccg cgtcgcgctg gtggaggcgg
     1741 tcggcggcgt ccgcgacgat cccgtcgcag acctcgtgca ggatgcgcag catcgccgtg
     1801 gcggccggcg gccggtagct gccgagcggc agggcggcgc gcacctcgcg ggagagcccc
     1861 ctcgcgtcga gcggccggac ggcgacgtcg cgccgcaccg tcgggagcgt cagccgcggg
     1921 atcagggcca ccccgacgcc ggcggcgacg agaccctcga ccgcgatccg gtcgtcggtc
     1981 tggaacgcga tgtggggttc gaagccgacg agccggcacg cccggggcag ctcctcgacg
     2041 gtcgacccgt ggcggactcc ctggatccag cggtcgccgg ccaggtcggc gagccgcacc
     2101 ctggcgcggt gggcgagcgg gtggcccggg ggcaggccga cgtggagggg gtcgtggacc
     2161 agcggcgcct gctcgaggtc ggtgcccatg ggctccgcga gctcgaactc gtagacgagc
     2221 gcgagctcga gctcgcgggc gcgcagcttg gggatgcact cgtcggggtc gccttcgaag
     2281 aggtcgagct cgacgtccgg gtactgccgc tggaaggccg cgatcgcccg cggcatgagg
     2341 gtggcgccgg cggtcgcgaa ccatcccagc cgcaggagcc cggcccgcag cccggcgatg
     2401 gcctggatct cgccctcggc ggcggccagc cggccgagga tctcgtcggc cccggccgcc
     2461 aggacgcgtc ccggctcggt gaggctgacg ccctgggggc cgcgaacgac gaggatcgcc
     2521 cctgcctccc gctccagtgc ggcgatctgc tgcgagacgg ccgacggcgt gtacgcgagc
     2581 gctctggccg ccgcggtgaa cgagccgcgc cgtgcgacct cacgcaggac cttcaggcgc
     2641 cggacgtcga gcatgaagca cagcttacag cagcttgcag gagctgtagc ttgcctgcac
     2701 agcggcgcgc cgctatcaag ggcggcagca accgtgggac cggccttgga ggagcgcatg
     2761 gcggagatcg acgtccacgg gccgcgtggc gcgggcgtcg aacggcgcct cgagcgcgtg
     2821 ctcggcctgc gggaggcggt cgcgctgggc gtgggcggga ccgtcggcgg cggcgtctac
     2881 gtgctcatcg gtgcggcggc cggtgaggcg gggcccgccg ccgtggcggc cttcgcgctc
     2941 gccttcctcg ccgcggcgct catcgcgctt ccctacgccg agctggcctg ccggtacccg
     3001 ctcgccggcg gcggctacgc cttcgcgcgc gccgtcttcg ggccgcactg gggcttcctc
     3061 atggggtggg gctactgggg ggcgtacgtc ttcgtctccg ggtacgtgac cgtgggcttc
     3121 ggggggtatc tcgcggcggt caccgggatc ccgccggtgg ccggcgcgct cgggctgatc
     3181 gccgcgtgcc tcggcctcaa cctgctcggc gtcagggcct cgggccggac gcaggcggcc
     3241 gtggtcggcg tggcgctgct cggcctcgtc ggcttcggcg tgtggggcct gccgagcgtc
     3301 cgggcggcgc gcttccaccc cttcctcccc cacgggccgc ggggcctgct gcccgcctcg
     3361 ctgctggcct tcctcgcctt cggcggcttc gacatggtcg ccgccgccgg ggaggagatc
     3421 gaccggcccg agcgcaacct gccgcgggcg atcctgctca ccctcgccgc ggtcctcggc
     3481 ctctacctgc tggtctcgct cgtcgccgtc ggcgtcgtcc cgagccacgc gctcggcgcc
     3541 tcccccgccc ccctggccga cgccgccgcc accttcggtg gcgcaccagc ccgccggctc
     3601 ctcgtgctga ccgcgctgct caccaccgcg gcgaccgcca acgcggtgct cgtggtcacc
     3661 tcccggatct cgttcgccat ggccagggac ggcctgctcc cgcgggcgct cgcggcggtc
     3721 ggctcgcgcg cgggcacgcc gtgggcgtcg ctcgtggcga acgcgctgct cctcggcgcc
     3781 gtcgccctca ccggctcggt cgccttcgcg gcggcggcag gcggcttcct ctacgtgctg
     3841 cacttcgtcc cgccgctcgt ggccctggtg gtgctgcgcc gccgccacga tgcggccccg
     3901 gcgttcagga ccccgctgcc cgccgtcgtc ctccccctcg ccttcgccgc cgccgcggtg
     3961 ctggtcgccg cgagcggcac gaccggcgcc gccgccgggc tcggctggct cgccgccggc
     4021 ctcgcggccc accgcctgtc cccccgcccg cccgtcacat agaggagcac ctcgcccatg
     4081 cccgggccgg cagtagcagc cacccggccg ctctacgacg cggatcagct tgcggcctgg
     4141 gcgggtggca ggaatggaat ctgctggttt cgcacccgga agctcacggt gcggaccttt
     4201 gagttgttcg ctctcgcgac gagtcggtag ccgcccgcct tggggaactg tgtgagggtg
     4261 ttgatagcca gcacggcgcg ttgatcagcg ccgtgctggg caccgggagg acgaccgact
     4321 tccacctcgc cgtccatctg gacgacggga ggaccgccat cctcgtactc gatccggatc
     4381 tggaaccggt gggtacggtt ggtctccgtc catggcacca ggacgctgac ggcgatgccg
     4441 aagtgcaacg gcgccgatgg cccctcgccg tcggggggga aggccagagt cgcatcggtc
     4501 caaccgcctc ctgagaggta caggaggccg ttgtgagctt ctgcgtggtt agccaggatg
     4561 gcgtactcga tccgtggtgt tgggtcacgt ggggctctgg cgagcgatca ccggctggtc
     4621 cgccgacccg gaggacgtgg actggtacgg caagtggtag ggggacacgg agatgtcgct
     4681 gatggaggaa ggcgaggttg cgaagcagct gcacgaccac cgaggggacc cagaagagtg
     4741 ggaagacact ccagcccgtg tgaaggtgca gcccgcgcga tctgaggttg tgtcgttcag
     4801 gctgccatcc gatcagctgg acgtgatcga gggcctcgct gaacaggctg gccagtccat
     4861 ctcggagctc gtccgacatg ctgtgctggc ctacgtcaac tggagagacg atggagccct
     4921 tccttgacgt gcgagctggg gcttccggag aattacggct caccatgcga acggccgtcc
     4981 gcggctccgg ctctacctcg aacgacctgg tggttcccga ctttccattg aagacggtga
     5041 cctgcgtcga ctgatgcgcc cggccgcggg gcgtcctacg tcgcttccgc cgatgcgtcc
     5101 ccggccgcgg caccaccggc ccggcgggcg gcctgctgct cggcttggcg cagctcggcg
     5161 cggcggatct tgccggtcag ggtcttcggc aggtcgtcga cgaactcgac ccggcgcggg
     5221 taggcgtagg cggacaggcg cgtgcggacg tggtcctgga gctccttgac gagcccgtcc
     5281 ccgggctcgt ggccgccggc caggcggacg aacgccttca cgacgttgcc ccgctgcgcg
     5341 tcggggctgg cgacgacggc cgactcggcg acggtagggt gctcgagcag gacgctctcg
     5401 acctcgaagg ggccgatccg gtagccggac gcgatgatga cgtcgtcggc ccggcccgag
     5461 taccagacgt agccgtcctc gtcctggacc gcggtgtcct tggtgtggaa ccacgtgccg
     5521 ccgaagacct cctcggagtc gtccggccgg ttccagtagc cgagcgggta gtgcgggttg
     5581 gacctggcac gcaggcagat ctcgccggcc tcgccgcgcg ggacgggctg ctcgtccgcg
     5641 tcgaggacgg ccacgtccca ccctggcatg ggcaggccca tcgacccggg ccggacctcg
     5701 acggtgggga agttcccgca cagcgggtag ctctcgctca ggccgtagta gtcgagcacc
     5761 ggacagccga tcgcccgcgc ccaccaggcg atcgcctccg ggttcagcgg ctcgccggcc
     5821 gagcaggcca gccgcagcgc gaagtccagg cgctccgccc cgtcgacgcg catcatggcc
     5881 cggatcgcgg tcggcgtggt gaacaggttc ccgacgccgt ggtcggcgat caggcggagg
     5941 gccttgccgg ggtcgaaccc gccccggcgg ccgtggacga gcgtctcgac gccgaagcgc
     6001 caggggccga ggatcccggg cacgatcccg gcgatccacg cccactcgcc agtcgagtgg
     6061 aacagctcgc ccgcacggac gtcgtggcag aactggaact cctcgtggct gaggacgtac
     6121 cggtgggcgt gcaggatccc cttggccagc ccggtcgtgc ccgacgtgta gtagagctgg
     6181 gccggggtgt ccgggtcggt cgccggggtg gcgaactcgg gcgaggcgtc ccgggtcagc
     6241 tcctcgaagc tctccccgcc ggtgccgccc acgacgacga cccgctcgac cgacggcgcc
     6301 tgctccacca tcgcggccac ccggtcgcgg tgctcggccg acgtgaccat caccttcgcc
     6361 tgcgagtcgt tgaggcggtg gacgacgccc tcgtcgccgt acagcacgct cagcgacaag
     6421 aggatcgcct cgcacttgta ggtggccagg aacgtcgccg cgatctcggg ggaggcgggg
     6481 gccatgaccg cgacccggtc gcccttgccc acgccgagcc cgcggagcag gttcgcggcc
     6541 cggtcggtga gccgccggac gtccccgaac gtccaccgct cgtcccgccc gtcgtcggac
     6601 acgaaccgca tcgccggccg gtccggctcg tgcttgtcga gcacgtcggc cgcgatgttg
     6661 taggcggccg gcagctcgaa gcggaacgag gaggtcatct cctcgtacgt ccctgggtgg
     6721 tgggtgcgat agtcccgcat ggcggtcgcc ctcctcacgg tcggccaacc agtatcgcat
     6781 ccgcgagaag ttgaggtccg cgcggccgga cgatagcttc ggcgtcatgt acgagacccg
     6841 ctggcctcgc ccggggcccg cgatgctggc cgtcggcgtg gtcgccggcc tcgtcgtcgg
     6901 aggcatcctc ggcatcagct tccctgcctc gagctccgac cggcacgcgg cggcgccgac
     6961 gaccagccag gtgacgcagt cctcggcgca gctgcccagg agcttcttca cgctgatcct
     7021 ggcgagcccg agcaccagcg cggacgccca gtcgaagctc gccaggtacc aggccgaggg
     7081 ggtcgccgac gcggtcatcc tgagcagggc gagctatccc gacctccgga cgcagttcgc
     7141 ggtgtgcgcc ggcacctacc cgagcaggac ccgcgccgac caggcgctgc aggagctgaa
     7201 ggcgcagctg cccaacctga cctcctcgcc ctacgtccgg ccgatgcgcc ggaccaccgg
     7261 gtagcgccgc cgacggcggc gccgggcgcg cccggcgacc gcgggaccag ccccgggaac
     7321 gccctggcgc ccgccttggc cgccggcccg gggaagggcg cgtcgtaggc ggccccgacc
     7381 tcgtcgcctg ggtcgccggc gcagccacgg cggacgatca ggctgaccgg caggtccccg
     7441 gtgcgctcga cgaacgcggc gaaccgcgac gccaccagct cggtgtgccg gtcgcagccg
     7501 taccaggacc ggtcgaccgg cttgtcggac cggccgaatc cggccaggtc gggcgcgatg
     7561 cagcggaagc cggcgtcgcg gacggcgtcg gtcatcccgc cgcctccccg gccggcggcg
     7621 cgccggagac gagctcttcg tagaggcggg cggtctggcc cgcgatcgcg ggccagctga
     7681 actcgtcgag cgcccgccgg cgtccggcct tgccgaagcg ctcggcctgg tccgggtcgg
     7741 cgagcaggcg gttgacccgc tcggcgaagt cggcggcgaa gcgctccggg tcccgcggcg
     7801 cgccgccgct gccctcgaac ggcaccagaa ggccgg
//
LOCUS       DASPYY010000434         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2462312, whole genome shotgun sequence.
ACCESSION   DASPYY010000434 DASPYY010000000
VERSION     DASPYY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462312"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VEP73_06780"
     CDS             <1..587
                     /locus_tag="VEP73_06780"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYT26170.1"
                     /translation="TYEGTVKVKLGPISMSYRGTVVLEDVDEAAHSAKLSASGKDVRG
                     GGTARAGVDTKLEAVSDTRTRMSVVSDVQLTGKVASFGRGSIQDVSARLFGQFADCLR
                     ETLESETRSAAAATGDGQTAASTAGAPAAAGGATPAAPAAAAPAPAEAAGAGPASAAT
                     PLKAGSLIGTVVAGRLKALWAALAGLFRRRSRRR"
     gene            729..1604
                     /locus_tag="VEP73_06785"
     CDS             729..1604
                     /locus_tag="VEP73_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT26171.1"
                     /translation="MHPPRFDYLAPRSLEEALDFLAGHGDDAKVLAGGQSLIPVLKLR
                     FASPEWLVDINRIPGLDGIAEEDGHLRVGALVRHRDAERSEVLRERYPTMAAAAPQVS
                     DPLVRNLGTVCGSLAHADPSGDWGAVMLAMGADLVAVSTSGSRTIAAREFFDGPFTTA
                     LRPDELLVEARVPTRPSTGGAYLKLERKVGDFATAAVAIRVSMSGGRIGDAGIGLTAV
                     GPQNLEATEAEAVLAGAAPSDEAFREAAELAARAASPTADMRGTVEYKRNVVRVFTER
                     GLRQAAAMAAADGGA"
     gene            1604..2137
                     /locus_tag="VEP73_06790"
     CDS             1604..2137
                     /locus_tag="VEP73_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT26172.1"
                     /translation="MQVTVNVNGSSRTADVEPRLLLVHLLRERLRLTGTHVGCDTTSC
                     GACTVLLDGTPIKSCTLLAVQADGRSVRTVEGLAEGGRPSAMQAAFKEEHGLQCGFCT
                     PGMMLTAEALLAENPDPTEEEVRWALSGNLCRCTGYVNIVRAVRAAGATMRSAAAAEP
                     TGPPEPVTAPAMTGGAQ"
     gene            2170..4479
                     /locus_tag="VEP73_06795"
     CDS             2170..4479
                     /locus_tag="VEP73_06795"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HYT26173.1"
                     /translation="MRRKEDDRFLHGRGTYVDDVQLPGMLHMAILRSPVAHARVTRID
                     VEQAKAHPGVVAVVTGADLDAHGLAWMPTLSADTQAVLALEKVVFQGQEVAAVVAEDP
                     YVAADALELIDVDYEPLPAVVDPRRALEPDTPLIREDKEGQTGNHIFHWEKGDKEATD
                     RAFAEADKVVSLDTFYPRCHPAPLETCGCVADVDPVTGKCTIWLTSQAPHAHRTLFAL
                     VAGLPEQNIRIISPDLGGGFGNKVPIYPGYVIATAASLLIGRPVKWVEDRTENLISTG
                     FARDYHMRGEMALTADGRITALRSQVIADHGAFNAAAQPSKFPAGLFHIFTGAYDVPA
                     AHCEVDGVYTNKAPGGIAYRCSFRVTEASYLIERLVQNAAVELEMDPAELRRRNFIQP
                     EQFPYRSAVGWEYDSGDYPRALDLALEKLGYDELRAEQEERWARGELMGIGIASFTEV
                     VGAGPGAEYDIAGLRMFDSAELRIHPTGKAILKIGVQTQGQGHETTFAQIVAGELGIP
                     PEDVTVMHGDTDNTPYGLGTYASRSTPVGGAATAMVARKLRDKATKIAAHLLEAAEED
                     VGYADGGFSVRGAPERAATIQDVAFAAYSNLPPGMEAGLEGVTYYDPPNMTFPFGTYC
                     VVVDIDKGTGRWTVRRMVAVDDCGVRINPMIVEGQIQGGLTEGFGIAAMELITFDEEG
                     NCIGQNFTDYLLPTSWETPRFETFQTVTPSPHHPIGAKGVGESATVGSPAAYVNAVLD
                     ALAPLGITNIDMPVTSDKVWAAMHEKGFV"
BASE COUNT          651 a         1679 c         1599 g          563 t
ORIGIN      
        1 acacctacga gggcacggtc aaggtcaagc tcggcccgat ctcgatgtcc tacaggggca
       61 cggtggtcct cgaggacgtc gacgaggccg cgcacagcgc caagctctcc gccagcggca
      121 aggacgtgcg cgggggcggc accgccaggg ccggcgtgga cacgaagctc gaggcggtct
      181 ccgacacccg cacgcgcatg tcggtggtca gcgacgtcca gctcaccggt aaggtggcct
      241 cgttcggccg gggctcgatc caggacgtct cggccaggct gttcgggcag ttcgcggact
      301 gcctccgcga gacgctggag tccgagacgc ggtccgccgc cgccgcgacc ggcgacggcc
      361 agaccgccgc ctccacggcc ggggccccgg cggcggccgg gggggccacg cccgccgctc
      421 cagcggccgc cgccccagcg ccagccgagg ccgccggtgc cgggccggcc tccgccgcca
      481 cgccgctcaa ggccggcagc ctgatcggca cggtcgtggc cggacgcctg aaggccctct
      541 gggccgccct cgccggcctg ttccggaggc gctcgcgcag gcggtgaccg ggcgccggcc
      601 ggcgacgggc ctctgcaaga cgggccttgt gcaatcggtc ggggtgggca agcatgcttg
      661 cgtgggcaag catgcttgct tggacggtcc ggtgacgccg ccgccgtgac ggcggctggg
      721 aggtcgcagt gcaccctccg cggttcgact acctcgcgcc ccgcagcctg gaggaggcgc
      781 tcgacttcct ggccggtcac ggggacgacg ccaaggtgct ggccggcggg cagagcctga
      841 tcccggtgct caagctccgc ttcgcctcgc ccgagtggct ggtggacatc aaccggattc
      901 ccggcctgga cgggatcgcc gaggaggacg gccacctgcg cgtcggcgcc ctggtccgcc
      961 accgcgacgc cgagcgctcc gaggtcctgc gcgagcgcta cccgaccatg gcggcggcgg
     1021 ccccgcaggt ctccgacccg ctcgtccgca acctcggcac ggtctgcggc tccctcgcgc
     1081 acgccgaccc gtcgggcgac tggggcgccg tcatgctggc gatgggcgcc gacctcgtgg
     1141 ccgtctccac ctccgggtcc cgcacgatcg cggcccgcga gttcttcgac gggccgttca
     1201 cgaccgccct gcggcccgac gagctgctgg tcgaggcccg cgtcccgacc cggccctcga
     1261 ccggcggcgc ctatctcaag ctcgagcgga aggtcggcga cttcgccacc gcggccgtcg
     1321 ccatccgggt cagcatgtcg ggcgggcgca tcggcgacgc cggcatcggc ctcacggcgg
     1381 tcggcccgca gaacctcgag gcgaccgagg cggaggccgt gctggccggc gccgcgccct
     1441 ccgacgaggc gttccgggag gcggccgagc tggccgcccg cgccgcctcg ccgaccgccg
     1501 acatgcgcgg gacggtggag tacaagcgca acgtcgtccg cgtcttcacc gagcgcggcc
     1561 tgcgccaggc cgccgccatg gccgccgccg acggaggtgc gtgatgcagg tcaccgtcaa
     1621 cgtgaacggc agcagccgga ccgccgacgt cgagccccgc ctgctgctcg tccacctgct
     1681 ccgcgagcgc ctgcggctca ccggcaccca cgtcggctgc gacacgacct cgtgcggggc
     1741 atgcacggtc ctgctcgacg ggacgccgat caagtcgtgc accctcctgg ccgtccaggc
     1801 cgacgggcgg agcgtccgga ccgtcgaggg actcgccgag ggcggccggc cgagcgccat
     1861 gcaggccgcg ttcaaggagg agcacggcct gcagtgcggc ttctgcaccc cgggcatgat
     1921 gctgaccgcc gaggcgctgc tggccgagaa ccccgacccc accgaggagg aggtgcgctg
     1981 ggcgctgtcc ggcaacctct gccgctgcac cgggtacgtg aacatcgtcc gggcggtgcg
     2041 ggcggccggc gccaccatgc ggtcggcggc cgccgccgag cccaccgggc cgcccgagcc
     2101 cgtgaccgcc ccggcgatga cgggaggcgc acagtgaccg gatcccccgt ccagggcctc
     2161 ggccatccga tgcggcgcaa ggaggacgac cgcttcctcc acggcagggg cacctacgtc
     2221 gacgacgtgc aactgccggg catgctccac atggcgatcc tgcgcagccc ggtggcccac
     2281 gcccgggtca cccggatcga cgtcgagcag gcgaaggcgc accccggcgt ggtggcggtc
     2341 gtcaccggcg ccgacctcga cgcgcacggc ctggcctgga tgccgaccct ctccgccgac
     2401 acccaggcgg tgctcgcgct cgagaaggtc gtgttccagg gccaggaggt cgccgccgtg
     2461 gtggccgagg acccgtacgt cgccgccgac gccctcgagc tgatcgacgt cgactacgag
     2521 ccgctgccgg ccgtggtcga cccgaggcgg gccctggagc cggacacgcc cctcatccgc
     2581 gaggacaagg agggccagac cggcaaccac atcttccact gggagaaggg cgacaaggag
     2641 gccaccgacc gggcgttcgc cgaggccgac aaggtcgtca gcctcgatac gttctacccg
     2701 cgctgccacc cggcgccgct cgagacgtgc ggctgcgtgg ccgacgtcga cccggtcacc
     2761 gggaagtgca cgatctggct cacctcgcag gcgccccacg cccaccgcac gctgttcgcc
     2821 ctggtggccg ggctccccga gcagaacatc cgcatcatct cgcccgacct cggcggcggg
     2881 ttcggcaaca aggtgccgat ctaccccggg tacgtcatcg cgacggccgc gtcgctcctg
     2941 atcggccggc cggtgaagtg ggtcgaggac cggaccgaga acctcatctc gaccgggttc
     3001 gcccgcgact accacatgcg cggggagatg gcgctgacgg ccgacggccg gatcacggcg
     3061 ctgcgctccc aggtgatcgc cgaccacggc gccttcaacg cggccgccca gccgtcgaag
     3121 ttccccgccg gcctgttcca catcttcacc ggggcctacg acgtgcccgc cgcccactgc
     3181 gaggtcgacg gcgtctacac caacaaggcg ccgggcggca tcgcctaccg ctgctcgttc
     3241 cgggtcaccg aggcctcgta cctgatcgag cgcctggtcc agaacgcggc cgtcgagctc
     3301 gagatggacc cggccgagct gcggcggcgc aacttcatcc agcccgagca gttcccctac
     3361 cgatcggcgg tcggctggga gtacgactcc ggcgactatc cgcgggcgct cgacctggcc
     3421 ctcgagaagc tcggctacga cgagctgcgg gccgagcagg aggagcggtg ggccaggggc
     3481 gagctgatgg gcatcggcat cgccagcttc acggaggtgg tcggcgccgg ccctggcgcg
     3541 gagtacgaca tcgccgggct gcgcatgttc gactcggccg agctgcgcat ccaccccacc
     3601 ggcaaggcga tcctgaagat cggtgtccag acccagggcc agggccacga gacgaccttc
     3661 gcccagatcg tggccgggga gctcggcatc ccgcccgagg acgtcacggt catgcacggc
     3721 gacaccgaca acacccccta cgggctcggg acgtacgcgt cccggtcgac cccggtgggc
     3781 ggggcggcga ccgcgatggt cgcccgcaag ctgcgcgaca aggcgacgaa gatcgccgcc
     3841 cacctgctcg aggccgccga ggaggacgtc gggtacgccg acggcggctt ctcggtccgg
     3901 ggcgctccgg agcgggcggc caccatccag gacgtcgcgt tcgcggcgta ctcgaacctc
     3961 ccccctggca tggaggccgg gctcgagggc gtcacgtact acgacccgcc caacatgacg
     4021 ttcccattcg ggacgtactg cgtagtcgtg gacatcgaca agggcaccgg ccggtggacc
     4081 gtccggcgga tggtcgcggt cgacgactgc ggcgtgcgga tcaacccgat gatcgtcgag
     4141 ggccagatcc agggcggcct cacggagggc ttcggcatcg ccgcgatgga gctgatcacc
     4201 ttcgacgagg aggggaactg catcggccag aacttcaccg actacctcct gccgacctcg
     4261 tgggagaccc cgaggttcga gaccttccag acggtgacgc catcgcccca ccacccgatc
     4321 ggggcgaagg gggtcggcga gtcggcgacc gtcggctccc cggccgccta cgtcaacgcg
     4381 gtcctggacg cgctcgcgcc gctcgggatc accaacatcg acatgccggt gacgagcgac
     4441 aaggtttggg ccgcgatgca cgaaaagggc tttgtgtagg gaaggcatgc ag
//
LOCUS       DASPYY010000435         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2462438, whole genome shotgun sequence.
ACCESSION   DASPYY010000435 DASPYY010000000
VERSION     DASPYY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462438"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VEP73_06800"
     CDS             <1..335
                     /locus_tag="VEP73_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYT26174.1"
                     /translation="GGDPNWGRILQAAGTAGVAFDPAAVRLEIGGGPGREGPGVAGAA
                     EGGGPGPELPVALVRHGAPTGADASAAMAGPDVRIRLHLGGPGPWACVRTTDLTPEYV
                     RLNSEYTT"
     gene            332..1276
                     /gene="argB"
                     /locus_tag="VEP73_06805"
     CDS             332..1276
                     /gene="argB"
                     /locus_tag="VEP73_06805"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYT26175.1"
                     /translation="MTALAGPRTQDLQAKAAVLHKALPWIKRFTGRTVVVKYGGAAMG
                     GGPGGRRAGVPDEDDGSLFDSFVDDVALLRYVGVDIVVVHGGGPEISQLMERLGKEPV
                     FVDGLRVTDADTMDLVRMVLVGRVNKALVGRLNTHGRLAVGLSGEDGLLVTARPRPGP
                     RGRDLGFVGDVEAVDPTALRALTAVGLIPVVATVAAGPMGQPYNVNADAVAGALAAAL
                     AAEKLVYLTDVEGLYADLGDPGSLIERTTLAELEAQLAAGRFRAGMIPKLEGVVAALR
                     AGVGSAHILDGRRRHALLLELFTDSGVGTMISPGPERR"
     gene            1282..>2045
                     /locus_tag="VEP73_06810"
     CDS             1282..>2045
                     /locus_tag="VEP73_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT26176.1"
                     /translation="MTDIADREAAVLWPTYARPPVQFVRGDGVRLYDAEGREYLDFLG
                     GIAVVAAGHAHPRVVAAVAGQLATLGHTSNLYFTGPQVDLAERLVDRFGPPAKVFLSN
                     SGAEANEAAIKVARRWGRATRGGRATGIVAMTGGFHGRTLATLAATGKPALHATFEPM
                     PAGFSHVPFGDAAALRAAIGPDTAAVLVEPIQGESGVVVPPPGWLRAVREITAEAGVL
                     LMVDEIQSGMGRTGRLFAHSHEGIRPDVVTMAKALG"
BASE COUNT          243 a          786 c          769 g          247 t
ORIGIN      
        1 acggcggcga ccccaactgg ggccggatcc tgcaggcggc aggcaccgcc ggggtcgcgt
       61 tcgacccggc ggcggtgcgc ctcgagatcg gcggcgggcc cggccgggag ggcccgggcg
      121 tggcgggggc cgccgagggc ggcgggccgg gccccgagct gccggtggcg ctggtccgcc
      181 acggggcgcc caccggcgcg gacgcgtccg cggcgatggc aggccccgac gtgcgcatcc
      241 gcctccacct cggcggcccc ggcccgtggg cgtgcgttcg caccaccgac ctcacccccg
      301 agtacgtgcg gctcaactcc gagtacacga catgacggcc ctcgccggcc cccgcaccca
      361 ggacctgcag gccaaggcgg ccgtgctgca caaggcgctg ccgtggatca agcgcttcac
      421 cggccgcacg gtggtcgtga agtacggtgg cgcggccatg ggcgggggcc cgggcgggcg
      481 gcgggccggc gtcccggacg aggacgacgg gtccctgttc gactccttcg tcgacgacgt
      541 cgccctgctc cgctacgtcg gggtggacat cgtggtggtc cacggcggcg gacccgagat
      601 cagccagctg atggagcgcc tcggcaagga gccggtgttc gtcgacggcc tgcgggtcac
      661 cgacgccgac acgatggacc tggtccgcat ggtgctcgtc ggccgggtca acaaggcgct
      721 ggtcgggcgc ctgaacaccc acggccggct ggcggtcggc ctctcgggcg aggacggcct
      781 gctggtcacc gcccgccccc gtcccggccc gcggggccgc gacctcggct tcgtcgggga
      841 cgtcgaggcg gtcgacccga ccgccctgcg tgcgctcacc gccgtggggc tgatccccgt
      901 ggtggccacc gtcgcggccg ggccgatggg acagccctac aacgtcaacg ccgacgcggt
      961 ggcgggcgcg ctcgccgccg cgctcgccgc ggagaagctc gtctacctga ccgacgtgga
     1021 gggcctgtac gccgaccttg gcgaccccgg ctccctcatc gagcgcacca ccctcgccga
     1081 gctcgaggcg caactcgccg ccgggaggtt cagggccggg atgatcccca agctcgaggg
     1141 cgtcgtggcc gcgctgcggg ccggcgtggg cagcgcccac atcctcgacg gccgccgccg
     1201 gcacgcgctc ctgctcgagc tgttcaccga ctcgggggtg gggaccatga tcagcccggg
     1261 cccggaacgg aggtgacccg gatgaccgac atcgccgacc gggaggcggc cgtgctgtgg
     1321 ccgacgtacg cccgcccccc ggtgcagttc gtccgcggcg acggcgtccg cctctacgac
     1381 gccgaggggc gcgagtacct ggacttcctc ggcggcatcg cggtcgtggc ggccgggcac
     1441 gcccacccca gggtggtcgc cgcggtggcg ggacagctcg ccaccctggg ccacacctcg
     1501 aacctgtact tcaccgggcc gcaggtcgac cttgccgagc ggctcgtcga ccggttcggc
     1561 cccccggcca aggtgttcct ctccaactcg ggcgccgagg ccaacgaggc cgcgatcaag
     1621 gtggccaggc gctggggcag ggccacgcgg ggcgggcggg ccaccgggat cgtggccatg
     1681 accggcggct tccacggccg cacgctggcg accctggccg ccacgggcaa gcccgccctc
     1741 cacgcgacgt tcgagcccat gcccgccggc ttctcccacg tgccgttcgg cgacgccgcg
     1801 gcgctccggg cggcgatcgg cccggacacc gccgccgtcc tggtcgagcc gatccagggc
     1861 gagtccggcg tcgtcgtccc gccgcccggc tggctgcggg cggtccggga gatcaccgcc
     1921 gaggcgggcg tcctgctcat ggtcgacgag atccagtcgg gcatgggccg caccggccgc
     1981 ctgttcgccc actcgcacga gggcatccgg cccgacgtcg tcacgatggc caaggccctc
     2041 ggcag
//
LOCUS       DASPYY010000436         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2321391, whole genome shotgun sequence.
ACCESSION   DASPYY010000436 DASPYY010000000
VERSION     DASPYY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321391"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="dapF"
                     /locus_tag="VEP73_06815"
     CDS             complement(<1..765)
                     /gene="dapF"
                     /locus_tag="VEP73_06815"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYT26177.1"
                     /translation="MEFVKAHGTGNDFVVVEDLADRHGLSAEFVRAVCDRHFGVGGDG
                     LIRIAPGTRAPFFMDYRNADGSRAEMCGNGARVVGKYLGDRGYVGSEFDLETPAGVVH
                     LELHAADDGCIDRVTVDMGAPAFDRASLPMLGQGEALKEQLEAGGQTVVATCLSMGNP
                     HAVVFVDDVDAAPVTTLGPVLETHPAFPGKTNVEFVQVLADDLLRQRTWERGVGETLA
                     CGTGACAVAVAGQLRGLTGRRVAIELRGGRLELEWAP"
     gene            complement(771..1724)
                     /gene="miaA"
                     /locus_tag="VEP73_06820"
     CDS             complement(771..1724)
                     /gene="miaA"
                     /locus_tag="VEP73_06820"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYT26178.1"
                     /translation="MSGPVAPQRRPGPVLALVGPTAAGKTALALDLAPRLGAEVVSAD
                     AMLVYRGMDIGTAKPTPAERAAVPHHLVDVVDPSEEFSVARFQPMARAAIADVLARGR
                     VPLLVGGSGLYFHAVVDEFAFPPTDAAVRGRLEAEAAGLAGLPALYQRLARCDPAAAA
                     RIQPGNLRRVVRALEVIELTGKPFSSFRGAMDQPVSRYRLTVLGLDPGPERLRARIAD
                     RVAAMAAGGLVDEVRRLAAWPLSRTARQALGYKEVLDALEQGGAISDALAAVVARTRA
                     YARRQLAWFRRDPRVRWSRLPEGPELAAWAFDELAEGPVEG"
     gene            complement(1721..>2227)
                     /locus_tag="VEP73_06825"
     CDS             complement(1721..>2227)
                     /locus_tag="VEP73_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26179.1"
                     /translation="ASPGAGAGDVPGVPHLAAATIPSALGPAATAAVGKELARAAGAL
                     EPAGVVGPAGVVAMGDLSARRTRAAPGSFHPAAAGFDAGVARALADGDLGALAALDPA
                     QAAELMVAGRAVLQALAGALAGARRVHGRVLYDDAPYGVGYIVATLAETAPSPAAAAG
                     AGGARSAR"
BASE COUNT          272 a          865 c          858 g          232 t
ORIGIN      
        1 gggcgcccac tcgagctcga ggcgcccgcc gcgcagctcg atcgcgaccc gccggcccgt
       61 gaggccgcgc agctggccgg ccaccgcgac cgcgcacgca ccggtcccgc aggccagcgt
      121 ctcccccacg ccacgctccc aggtgcgctg cctgaggagg tcgtcggcga ggacctgcac
      181 gaactccacg ttggtcttgc cggggaaggc ggggtgggtc tccagcaccg gcccgagggt
      241 ggtcaccggc gcggcgtcga cgtcgtcgac gaacacgacc gcgtgcgggt tgcccatcga
      301 caggcaggtg gccacgacgg tctggccgcc ggcctcgagc tgctccttga gcgcctcgcc
      361 ctgcccgagc atgggcaggg aggcccggtc gaaggcgggg gcgcccatgt cgacggtgac
      421 ccggtcgatg cagccgtcgt cggcggcgtg cagctcgaga tgcacgaccc cggccggcgt
      481 ctcgaggtcg aactcgctgc cgacgtagcc gcggtcgccg aggtacttgc ccacgacccg
      541 ggcgccgttg ccgcacatct cggcccgcga cccatcggcg ttgcggtagt ccatgaagaa
      601 gggggccctg gtcccggggg cgatccggat cagcccgtcg ccgcccacgc cgaagtgccg
      661 gtcgcagacg gcccgcacga actcggccga gagcccgtgg cggtcggcga ggtcctcgac
      721 caccacgaag tcgttgcccg tgccgtgcgc cttgacgaac tccacgctgc ctatccctcc
      781 accggtccct ctgccagctc gtcgaaggcc cacgcggcca gctccggacc ctcaggaagc
      841 ctactccatc ggacccgtgg gtcccgccgg aaccacgcga gctggcggcg ggcgtaggcg
      901 cgggtccggg cgaccaccgc ggccagcgcg tcggagatcg cgccgccctg ctcgagggcg
      961 tcgaggacct ccttgtagcc gagcgcctgg cgggccgtgc gcgacagcgg ccaggcggcc
     1021 aggcggcgca cctcgtccac gagccctccg gcggccatcg cggccacccg gtcggcgatg
     1081 cgcgcccgca gccgctccgg gccggggtcc aggccgagga cggtgagccg gtagcgcgac
     1141 accggctggt ccatcgcgcc ccggaacgag gagaacggct tgccggtgag ctcgatcacc
     1201 tcgagggcgc gcacgacgcg gcgcaggttg cctggctgga tgcgggcggc ggcggccggg
     1261 tcgcaccggg ccagccgctg gtacagcgcg ggcaggccgg cgaggccggc cgcctcggcc
     1321 tcgaggcggc cgcggaccgc ggcgtcggtg ggcgggaacg cgaactcgtc caccacggcg
     1381 tggaagtaca ggccggagcc gccgacgagc agcggcaccc ggcccctggc gaggacgtcg
     1441 gcgatcgcgg ccctggccat cggctggaag cgcgcgaccg agaactcctc gctcgggtcc
     1501 accacgtcga cgaggtggtg cggcaccgcg gcgcgctcgg ccggggtcgg cttggccgtg
     1561 ccgatgtcca tcccgcggta gacgagcatc gcatcggcgc tcacgacctc ggcgccgagg
     1621 cggggggcga gatcgagcgc aagcgccgtc ttgcccgccg cggtcggccc taccagggcg
     1681 aggaccggcc cggggcggcg ctggggagcc accgggccgc tcatcgagcg ctccgggcgc
     1741 ccccggcccc ggccgcggcc gcggggctcg gggcggtctc ggcgagggtc gcgacgatat
     1801 acccgacccc gtagggcgcg tcgtcgtaca gcacccggcc gtggacgcgc cgcgcgcccg
     1861 ccagcgcgcc cgccagcgcc tggagcaccg cccggcccgc gaccatcagc tccgcggcct
     1921 gggccgggtc gagcgcggcc agcgcgccga ggtcgccgtc ggcgagggcg cgcgccacgc
     1981 cggcgtcgaa gccggccgcc gccggatgga acgagcccgg ggcggcgcgt gtccggcggg
     2041 cggagaggtc ccccatcgcg accaccccgg ccggcccgac caccccggcc ggctcgagcg
     2101 ccccggccgc ccgggccagc tccttcccca cggcggcggt cgcggcgggt ccgagcgcgg
     2161 acgggatcgt cgccgctgcc aggtgcggga cgccggggac gtcgccggcg cccgcgccgg
     2221 gggaggc
//
LOCUS       DASPYY010000437         4152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2601516, whole genome shotgun sequence.
ACCESSION   DASPYY010000437 DASPYY010000000
VERSION     DASPYY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601516"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1903)
                     /locus_tag="VEP73_06830"
     CDS             complement(191..1903)
                     /locus_tag="VEP73_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYT26180.1"
                     /translation="MLVANRGEIAVRVLRACREEGLAGIAVYAEPDARALHVREASRA
                     VALGGTAVAESYLDAGRLLAAAREAGASAVHPGYGLLSENAAFARAVEAAGLVWIGPP
                     ADAIEQMGDKLAARRAVLAAGVEPVPGTTEPVRGADEVVAFGERHGWPVAVKAAAGGG
                     GRGIRVVRSPGEAGEALAAAAREAAAAFGDPACYLERFFERPRHVEVQVLADAHGGIV
                     HLGERDCSVQRRHQKLVEEAPAPGLPAAVRDRLAAWAVTVAKACGYVGAGTVEFLWDP
                     PSGGAWFLEMNTRLQVEHPVTEAVTGVDVVRAQLRVAAGEPLGLAQEDVHATGHAIEC
                     RVNAEDPGRGFLPSAGPLTRLVWPGGPGVRVDAGYEAGDVVSPFYDDLLGKVIAWGRD
                     RAEATARMAAALAATRVEGVASTVPALERVLASGDFARAAHWTTWLEETVDLGDLAAA
                     GPGGEPDLTVTVDGTPWPVRLYRHRPSDLAPSGAGAGRVVSPLAGTLVRVEVAPGQAV
                     AVGQLLAVVEAMKMETPLRAPFAGEVAAVQYAVGDAVSAGQVVVVVVEAGPQGGPGSE
                     EGRA"
     gene            complement(1978..3468)
                     /locus_tag="VEP73_06835"
     CDS             complement(1978..3468)
                     /locus_tag="VEP73_06835"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYT26181.1"
                     /translation="MRAGGAERYHARAREQGKLFVRERLRRLLDDADGFVEDGLLANA
                     TAGDLPADGVVTGVGTVGGRTVAVMANDPTVKAGSWGARTVEKIIRIEEYALRHEVPM
                     IWLVDSAGARITDQVEMFPGRRGAGRIFHNQVKLSGRVPQVCCLFGPSAAGGAYIPAF
                     CDVVFMVDGNASMYLGSPRMAEMVIGERTTLEEMGGAAMHCRVSGCGDLLAADEDEAL
                     RAARHYLSYFPGNWRSEPPVEDPRGPAYAGPVAPLVPLDEAQPFDMLPLVAAVCDEAS
                     FFEVKREFAPEIITGLARIEGRPVGIVANQPLAKGGVLFVDSADKAARFVWLCDAFNL
                     PLVFLADVPGFMIGSAVERQGIIRHGAKMISAVSEATVPKVCVVVRKAYGAGLYAMAG
                     PGFEPDATIALPTASIAVMGPEAAVNAVYYNKLAALPEAERPAEVARLQREYREDVDL
                     VRLAADLVVDAVVDPEDLRAEVARRLTAARGKDREFTRRRHGVPPV"
     gene            complement(3528..>4152)
                     /locus_tag="VEP73_06840"
     CDS             complement(3528..>4152)
                     /locus_tag="VEP73_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYT26182.1"
                     /translation="GADRLQVVVAATESYNRANLNRTVAETLDEIGGVVRLAGGTPVE
                     ATVSTAWGCPYEGDVPTDRVVGLAERLAALGCQAVSLGDTTGMATPTRVEALLGALAG
                     RVPAVNCHFHDTRGTGLANLVAALQAGCADVDTAVGGLGGSPSAPGAAGNVASEDAVH
                     MLEDMGVATGVDLDALLAAARVAAELVGHPLRGQVLVAGPRTRAVHR"
BASE COUNT          502 a         1636 c         1530 g          484 t
ORIGIN      
        1 gtgcagcacc gcgttggtga ccgcctcgct ggtgagcagg gcggcgacct ccgaggcggg
       61 cgcgtcgggg ccgagcgtgc ggaccacgaa gcgccgggcc gccccgacct ggtcggggcg
      121 cccgggaaac acccggtagg cgcgttcctc cttcatcgtc cccttcgaag tcggcatgcc
      181 ggcgaccccc ctatgctcgc ccttcctccg accctggacc gccctgcgga ccggcctcga
      241 ccaccaccac caccacctgg ccggcggaca cggcgtcacc gacagcgtac tgtaccgcgg
      301 ccacctcgcc cgcgaacggc gcccgcagcg gcgtctccat cttcatcgcc tcgacgacgg
      361 cgaggagctg gcccaccgcc acggcctggc ccggggcgac ctcgacccgg acgagggtgc
      421 cggccagcgg cgagacgacc cggccggcgc cggccccgct gggcgccagg tcggacggcc
      481 ggtggcggta gaggcgcacc ggccacggcg tgccgtccac ggtcacggtg aggtccggct
      541 cgccgcccgg tcccgccgcg gccaggtcgc ccaggtccac cgtctcctcg agccacgtcg
      601 tccagtgggc ggcgcgcgcg aagtctccgg acgcgaggac gcgctcgagc gcgggcacgg
      661 tggacgcgac cccctcgacc cgggtggcgg ccagggccgc cgccatcctg gcggtcgcct
      721 cggcgcggtc gcggccccag gcgatcacct tgccgagcag gtcgtcgtag aacggcgaga
      781 ccacgtcgcc cgcctcgtac ccggcgtcca cgcggacgcc cgggccgccc ggccagacga
      841 ggcgggtgag cgggccggcg ctgggcagga agccccggcc cgggtcctcg gcgttgaccc
      901 ggcactcgat cgcgtgcccg gtggcgtgga cgtcctcctg ggcgagcccg agcggctccc
      961 cggcggccac ccggagctgt gcccgtacca cgtcgacccc ggtgacggcc tcggtgaccg
     1021 ggtgctcgac ctggagccgg gtgttcatct cgaggaacca ggcgccgccg gacggcgggt
     1081 cccagaggaa ctcgaccgtg cccgccccga cgtagccgca cgccttggcg accgtgacgg
     1141 cccaggcggc gagccggtcg cgcacggcgg cgggcaggcc gggcgccggc gcctcctcca
     1201 ccagcttctg gtggcggcgc tgcaccgagc agtcgcgctc gccgaggtgc acgatgccgc
     1261 cgtgcgcgtc ggcgagcacc tggacctcga cgtggcgggg ccgctcgaag aaccgctcga
     1321 ggtagcacgc cgggtcgccg aacgcggcgg ccgcctccct ggccgcggcg gccagcgcct
     1381 cgccggcctc gccaggcgag cgcaccaccc ggatcccccg cccgccgccc ccggctgccg
     1441 ccttcaccgc gaccggccag ccgtgccgct cgccgaacgc gacgacctcg tccgcgccgc
     1501 ggaccggctc ggtcgtgccc ggcaccggct cgaccccggc ggcgaggacc gcccggcggg
     1561 cggcgagctt gtcgcccatc tgctcgatcg cgtcggcggg cgggccgatc cagacgagcc
     1621 cggccgcctc gaccgctcgc gcgaacgcgg cgttctcgct gagcagcccg tagccggggt
     1681 ggacggccga ggcgcccgcc tcccgcgcgg cggccaggag gcgcccggcg tccaggtagg
     1741 actcggccac ggccgtcccg ccgagggcga cggcccggct ggcctcgcgc acgtgcaggg
     1801 cgcgcgcgtc cggctcggcg tagaccgcga tcccggcgag gccctcctcg cggcaggcgc
     1861 gcagcacccg cacggcgatc tccccccggt tggcgaccag caccgcgccg aggcgccccg
     1921 tccggcggcg aggagccgta cccgtacggc ggcgaggagc tgcacggggt cccgccatca
     1981 gaccggcggg acgccgtggc ggcggcgggt gaactcgcgg tccttgcccc gtgccgccgt
     2041 gagccggcgg gccacctcgg cgcgcaggtc ctccgggtcg acgaccgcgt ccacgaccag
     2101 gtcggcggcg agccgcacca ggtcgacgtc ctcgcggtac tcccgctgca ggcgcgccac
     2161 ctcggcaggg cgctcggcct cgggcagcgc cgcgagcttg ttgtagtaga cggcgttgac
     2221 ggccgcctcc ggccccatga ccgcgatcga ggcggtgggc agcgcgatgg tggcgtccgg
     2281 ctcgaagccg ggcccggcca tcgcgtagag cccggccccg tacgccttgc ggacgaccac
     2341 gcacaccttg ggcacggtcg cctcgctgac cgcggagatc atcttggcac cgtgcctgat
     2401 gatgccctgg cgctcgacgg cgctgccgat catgaagccg ggcacgtcgg ccaggaacac
     2461 gagcggcagg ttgaaggcgt cgcacagcca gacgaacctg gcggccttgt cggccgagtc
     2521 gacgaacagc accccgccct tggcgagcgg ctggttggcg acgatgccga ccgggcggcc
     2581 ctcgatgcgg gcgaggccgg tgatgatctc gggcgcgaac tcgcgcttga cctcgaagaa
     2641 gctggcctcg tcgcagaccg ccgcgacgag cgggagcatg tcgaacggct gtgcctcgtc
     2701 cagggggacg agcggggcca ccgggccggc gtaggccggc cctctcgggt cctcgacggg
     2761 aggttcggac cgccagttgc cggggaagta cgacaggtag tggcgggcgg cccgcagtgc
     2821 ctcgtcctcg tcggcggcca gcaggtcgcc gcaccccgat acccggcagt gcatggccgc
     2881 cccgcccatc tcctcgagcg tggtccgctc gccgatcacc atctcggcca tgcgcggcga
     2941 cccgaggtac atcgaggcgt tgccgtccac catgaacacc acgtcgcaga aggcggggat
     3001 gtaggcgccg ccggctgccg acgggccgaa caggcagcac acctggggca cccggcccga
     3061 gagcttgacc tggttgtgga agatcctgcc cgcgccccgc cggccgggga acatctccac
     3121 ctggtcggtg atgcgagccc cggccgagtc gaccagccag atcatcggca cctcgtggcg
     3181 cagggcgtac tcctcgatgc ggatgatctt ctccaccgtg cgggcgcccc aggagcccgc
     3241 cttgaccgtc gggtcgttcg ccatcaccgc gaccgtccgg cccccgaccg tgccgacccc
     3301 ggtgacgacc ccgtcggcgg gcaggtcgcc ggctgtcgcg ttcgccagca gcccgtcctc
     3361 gacgaacccg tcggcgtcgt cgagcagccg gcgcagccgc tcgcgcacga acagcttgcc
     3421 ctgctcccgc gccctggcgt ggtagcgctc ggccccgccg gcccgcatgt ccttggcgag
     3481 cgcccggaac gccggctcgg gccgctccct ggcctccctg gggtcggtca ccggtgcacc
     3541 gcccgggtgc gcgggccggc gaccagtacc tggccgcgga gcgggtggcc gacgagctcc
     3601 gcggcgaccc gggccgcggc gagcagcgcg tccaggtcga cgccggtggc cacccccatg
     3661 tcctcgagca tgtggacggc gtcctcgctg gccacgttgc cggccgcccc tggcgcgctg
     3721 gggctgccgc cgagcccccc gaccgcggtg tcgacgtcgg cgcacccggc ctgcaacgcg
     3781 gcgaccaggt tcgcgaggcc ggtgccgcgg gtgtcgtgga agtggcagtt gaccgcgggc
     3841 acccggccgg ccagcgcgcc gagcagcgcc tcgacccggg tcggggtggc catgccggtg
     3901 gtgtcgccca gcgagaccgc ctggcagccc agcgcggcga gccgctcggc gaggccgacc
     3961 acccggtcgg tgggcacgtc accctcgtac gggcagcccc aggcggtcga gacggtcgcc
     4021 tccaccgggg tgccgccggc cagccgcacg accccgccga tctcgtcgag ggtctcggcg
     4081 acggtgcggt tcaggttcgc ccggttgtag ctctccgtgg cggccaccac gacctggagc
     4141 cggtcggcgc cg
//
LOCUS       DASPYY010000438         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2180854, whole genome shotgun sequence.
ACCESSION   DASPYY010000438 DASPYY010000000
VERSION     DASPYY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180854"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VEP73_06845"
     CDS             complement(<1..294)
                     /locus_tag="VEP73_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYT26183.1"
                     /translation="MQPPPFPTSRPRRLRRTRVLRDLARETSVSRRDLVAPLFVKEGI
                     REPAPISSMPGHRQHTLESLRKEAAELASLGIPALLLFGIPEVKDAEGSQAWAP"
     gene            complement(321..>1734)
                     /gene="cobA"
                     /locus_tag="VEP73_06850"
     CDS             complement(321..>1734)
                     /gene="cobA"
                     /locus_tag="VEP73_06850"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYT26184.1"
                     /translation="PPGLVEAAPDGCELVYAGKLPDRHVLPQAEINALLVDRALAGGR
                     VVRLKGGDPFVFGRGAEEAEACAAAGVPFEVVPGVSSAVAVPAYAGIPATHRDLSSAF
                     AVVTGHEDPAKEAAGALGGAGPGARWEALAQIGTVCVLMGVERLAAIAARLVEAGRAP
                     DTPAAVIEQGTTPAQRVVRATLATIAGEAGRAGVRSPAVVVIGEVVALRDRIAWYETR
                     PLFGRTVLVPRTREQAGALSELLRERGAEPLEVATIEVRPPSSPAELDRAVGLLAAGR
                     FDWVVLTSANGVAALRGRVEATGLDARALGGTLVAAVGPATEAALRSWGIAADLVPGV
                     ATTAALGEAFPASPGRVLLPRADLATPDLSPAIEAKGGHPDDVVAYRTVPLDDMDPAA
                     RKRLDGGEVDWVAFTAASTVTGFVRAYGGPPPAGVRAAAIGPVTAAAAEASGVRVDAL
                     AAEHTIPGLVAAIERAVAGT"
BASE COUNT          196 a          666 c          697 g          175 t
ORIGIN      
        1 cggcgcccac gcctgggagc cctcggcgtc cttgacctcg gggatgccga acagcagcag
       61 ggccgggatg ccgagcgagg cgagctcggc cgcctccttg cgcaggctct ccagcgtgtg
      121 ctggcggtgg ccgggcatgg acgagatcgg ggcgggctcg cggatgccct ccttgacgaa
      181 caggggcgcc acgaggtccc ggcgggagac cgaggtctcc cgcgcgaggt cgcgcagcac
      241 gcgggtgcgg cgcaggcggc ggggacggct ggtggggaag ggcggtggct gcatgtcact
      301 cctcggcggt cttgtcgatg ctacgtgccc gcgacggcgc gctcgatggc ggcgaccagg
      361 cccgggatgg tgtgctcggc ggccagggcg tcgacccgga cgccggacgc ctcggccgcc
      421 gcggcggtga ccgggccgat cgccgcggcc cggacgccgg ccgggggcgg gccgccgtag
      481 gcgcggacga acccggtcac ggtggaggcc gcggtgaacg cgacccagtc cacctccccg
      541 ccgtccagcc gcttgcgggc ggcggggtcc atgtcgtcga gggggacggt gcggtaggcg
      601 accacgtcgt ccgggtggcc gcccttggcc tcgatcgccg gggagaggtc cggggtggcc
      661 aggtcggccc ggggcaggag cacccggccc gggctggccg ggaacgcctc gccgagcgcc
      721 gcggtggtcg ccacgccggg gaccaggtcg gcggcgatcc cccacgagcg cagcgcggcc
      781 tcggtcgccg ggccgaccgc cgccaccagc gtcccgccga gcgcgcgcgc gtcgaggccg
      841 gtcgcctcga cccgcccgcg cagcgccgcg accccgttcg cgctggtcag gacgacccag
      901 tcgaaccggc cggcggcgag tagccccacc gcgcggtcca gttcggccgg cgaggagggc
      961 ggccgcacct cgatggtggc gacctcgagg ggctcggcgc cgcgctcgcg cagcagctcc
     1021 gagagcgcgc ccgcctgctc ccgggtcctc ggcaccagca ccgtccggcc gaacagcggc
     1081 ctggtctcgt accaggcgat gcggtcgcgc agcgcgacga cctcgccgat cacgaccacg
     1141 gcgggggagc ggacgccggc ccggccggcc tccccggcga tcgtggcgag cgtcgcccgc
     1201 acgacccgct gggccggcgt cgtcccctgc tcgatcacgg cggccggggt gtccggggcg
     1261 cggccggcct cgaccagccg ggccgcgatg gcggccaggc gctcgacccc catgagcacg
     1321 cagacggtcc cgatctgggc cagggcctcc cagcgggcac cggggccggc gccgcccagg
     1381 gcgcccgcgg cctccttggc ggggtcctcg tgcccggtga cgacggcgaa cgcgctcgac
     1441 aggtcgcggt gggtggccgg gatgccggcg taggccggga ccgccacggc cgaggagacc
     1501 ccggggacga cctcgaacgg gacccccgcc gcggcgcacg cctcggcctc ctcggcgccg
     1561 cgcccgaaca cgaaggggtc gccccccttc agccggacca cccgcccgcc ggccagcgcg
     1621 cggtcgacca gcagcgcgtt gatctccgcc tggggcagca cgtggcggtc gggaagcttg
     1681 ccggcgtaca ccagctcgca gccgtccggg gccgcctcga ccaggccggg gggg
//
LOCUS       DASPYY010000439         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1338566, whole genome shotgun sequence.
ACCESSION   DASPYY010000439 DASPYY010000000
VERSION     DASPYY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338566"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VEP73_06855"
     CDS             complement(<1..441)
                     /locus_tag="VEP73_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT26185.1"
                     /translation="MTALLEIDGLTKRFGGVVAVDGCSFAVPEGTVTALIGPNGSGKT
                     TVFNLVTGYLVPDGGSVAFRGRALRRPDPASLYRKGLARTFQQARVFPRLTVLDNLVL
                     AVQQPWTGMLRGRVGRADAARAGELLTDFGLDHLDGRLAGELSYG"
     gene            complement(438..1559)
                     /locus_tag="VEP73_06860"
     CDS             complement(438..1559)
                     /locus_tag="VEP73_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT26186.1"
                     /translation="MTAAPRPGRAVPAAGRLSGAGLARAAAVFGALAAAPFVAGEHWV
                     LNLGFFTLMYAVLGTAWNLLGGYAGYTSLGHAAFFGVGAYALALAFGHAAAGAGLAPF
                     VALPAIGVGTGLAALPVAWVALRTRATTFAIVTLTLLFVVQTLAFNLGSLTGGAQGIS
                     LPAPPFPVDTFERPFYLAMLALLAAAVLASWYVRGSKLGLALFGIRDDEDRARGLGVR
                     VTGVKLAAFGLSVGLTAMAGALWAYYVSFIYPQFAVDPLVSIGAVLMVYLGGRGTLWG
                     PVLGAFVLIPAQQYLAYRLGQSQLYLVGYAAVFLLVMLFLPRGILPSAADLLDRWRAG
                     RAERRPDPQGRAAGPGSDPARAAVPAGAPRASRQREEPP"
     gene            complement(1556..>1810)
                     /locus_tag="VEP73_06865"
     CDS             complement(1556..>1810)
                     /locus_tag="VEP73_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT26187.1"
                     /translation="GIVFPFNPGSHYDLISRLLSIVVLGGLGSLAGSVVAALLMGVAE
                     AVVAATISPVWSAFVFFAVLLGILVLRPQGLFGTTHRGAL"
BASE COUNT          287 a          637 c          729 g          157 t
ORIGIN      
        1 gccgtaggag agctcgccgg cgagccggcc gtccaggtgg tccaggccga agtcggtgag
       61 cagctcgccg gcccgcgccg cgtcggcccg cccgacccgg ccgcgcagca tgccggtcca
      121 gggctgctgc acggcgagga cgaggttgtc gagcacggtc aggcggggga agacgcgcgc
      181 ctgctggaac gtgcgggcga ggcccttgcg gtacaggctg gccgggtcgg gccggcgcag
      241 cgcccggccg cggaacgcga ccgagccgcc gtcgggcacg aggtagccgg tgacgaggtt
      301 gaacacggtc gtcttgcccg agccgttcgg gccgatcagg gctgtcaccg tgccctcggg
      361 gacggcgaac gagcagccgt cgaccgcgac caccccgccg aaccgcttgg tgaggccgtc
      421 gatctcgagc agggccgtca cggcggctcc tcccgctgcc tgctcgctcg cggcgcgccc
      481 gccgggacgg ccgccctcgc ggggtcggag ccggggcccg cggcccggcc ctgcgggtcc
      541 gggcgccgct cggcccgccc ggcgcgccag cggtcgagca ggtcagcggc tgacgggagg
      601 atcccccgcg gcaggaacag catgacgagc aggaacacgg cggcgtagcc gacgaggtag
      661 agctggctct gcccgaggcg gtaggccagg tactgctggg ccgggatgag gacgaacgcg
      721 ccgagcaccg ggccccacag ggtgccccgg ccgcccaggt agaccatgag cacggcgccg
      781 atggacacga gcgggtccac cgcgaactgc gggtagatga aggagacgta gtacgcccag
      841 agcgcaccgg ccatcgcggt gagcccgacg ctcaggccga aggcggcgag cttgacgccg
      901 gtgacccgga cgccgagccc gcgggcgcgg tcctcgtcgt cgcggatgcc gaacagcgcg
      961 aggccgagct tcgagccgcg gacgtaccag ctcgcgagca cggcggccgc cagcagggcc
     1021 agcatcgcga ggtagaaggg gcgctcgaag gtgtcgacgg ggaacggcgg ggcgggcagc
     1081 gagatcccct gggcgccgcc ggtcagcgac cccaggttga acgcgagcgt ctgcacgacg
     1141 aacagcaggg tcagggtgac gatggcgaac gtcgtcgccc gggtccgcag cgccacccag
     1201 gcgaccggca gcgcggccag gccggtcccg acgccgatcg cgggcagcgc gacgaacggc
     1261 gccagccccg ctccggcggc ggcgtggccg aacgcgagcg cgagggcgta ggcgccgacg
     1321 ccgaagaacg cggcgtggcc gagcgaggtg tagccggcgt agccgccgag caggttccag
     1381 gcggtgccca ggaccgcgta catgagggtg aagaacccga ggttcaggac ccagtgctcg
     1441 cccgcgacga agggagcggc cgcgagcgcg ccgaacaccg ccgcggcgcg ggcgaggccg
     1501 gcgccggaca ggcgccccgc ggccggcacg gcccggccgg gccggggcgc cgcggtcaca
     1561 gcgcaccccg gtgcgtcgtg ccgaagaggc cctgcggccg cagcaccagg atcccgagca
     1621 ggacggcgaa gaacacgaag gccgaccaga ccggcgagat ggtggccgcg acgaccgcct
     1681 cggccacgcc catgaggagg gcggcgacga ccgagccggc caggctgccg agccccccga
     1741 gcaccacgat ggagagcagg cgggagatga ggtcgtagtg gctgcccggg ttgaacggga
     1801 agacgatgcc
//
LOCUS       DASPYY010000440         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1619522, whole genome shotgun sequence.
ACCESSION   DASPYY010000440 DASPYY010000000
VERSION     DASPYY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619522"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VEP73_06870"
     CDS             complement(<1..1178)
                     /locus_tag="VEP73_06870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT26188.1"
                     /translation="MTTETVLPDALPGDLAAMLPPGNVVVLGGPGSGKTSLLEAMMPA
                     LVAGQAGGAAFLTSSRQAAVASSERLLAASPPAATGPLGCVTWHAFARGLVTSHADLL
                     SYRGEPRLLSGPEQWSLVRSLLLETAGVDWGDLAPLVGTRAFTDELADFVLACERRLI
                     GPDELLARVGAGGRPAWVPGAAFLSAYADRLALEDSVDQAGLVAQAGDLLEDQPEVLA
                     TTVARTGTVLVDEAQELDPAQSRLLELLVGGGARAVLAGDPAGTTDAFRGAAPGALPG
                     WAGRLGAAVVRLEHSRRLGGHGLDAVQRLQRGGHEPPVALGGGRTTAVEVARHLGPAD
                     EADALARLLRVAHQRDGVPYGRMAVLLPSVRRASGPIRRALERYGVPYRLGAGDRQLI
                     A"
     gene            1434..1979
                     /locus_tag="VEP73_06875"
     CDS             1434..1979
                     /locus_tag="VEP73_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HYT26189.1"
                     /translation="MLAAVVLGGCSPSTRAPAPLATPAAERRSWPGPPPGAATALVER
                     VVDGDTFVAVVGGRRDRVRVIGVDTPETVAPGRPVERYGRQASAFAKHWLTGATVRLA
                     GDVESRDRYGRLLAYVWLADGTFWNALLAAEGYGQQLTIPPNLAFAELLGRLVAEARS
                     HHRGLWAAGGRVTRGESGLRA"
     gene            complement(2354..3841)
                     /locus_tag="VEP73_06880"
     CDS             complement(2354..3841)
                     /locus_tag="VEP73_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYT26190.1"
                     /translation="MATPKNDPNETRPLRDPDAETRPVRPDPDPARTVPTWAPPVNGG
                     NGWGQHAGPSRDAPTWDGPSSGAPAWGAPASDAPTWDAPDSDAPTWGAPASDAPTWGG
                     ATSPGAQHAASGEVPGDRPAWTSTAVGGASPSGGVPGWGGPPPGSGVAPRPRRGAGRA
                     LAATIAALLLVLTGVGLTLLVDRRDSSAGVPAGAGVPVAAQPAPLPAGEEPVAAVART
                     LGPSVVQIETDQGLGSGVIYDRNGYVLTAAHVVEGSGTVTVRLYDGSTLRGQVLGIDQ
                     GNDIAVVKVDRGGLKPAALALGVDVQVGQTAVAIGSPFGLQQTVTAGVVSAKNRSLAT
                     GNGGALEVIQTDAPINPGNSGGALADRSGRVIGINDSIRSESGGNEGVGFAIPIDTAA
                     SSASRIVKGEKIQSGYLGVSLSTPTLGRGGALIDQVLPGSPAEKAGIQVGDLVIEFNG
                     KAIQSSDDLAAQVRLAAPGQKVGVKLVRGGKEQTVTAALTERPIR"
     gene            4097..4891
                     /locus_tag="VEP73_06885"
     CDS             4097..4891
                     /locus_tag="VEP73_06885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYT26191.1"
                     /translation="MGQAERPGAARDQGRRGFLRGAVATGLAAAILATSGREAEARHA
                     TGPGDLAAATVPPWPEPYLGQLSITWRVATGRRAVAISFDDGPDPRWTPAVLDALAHA
                     GARATFFCCGQAARRHPGLVRRAAAAGEIGNHSWSHPDLAALSSLQVDREIDRTHDLL
                     GGILGRPPTLLRPPYGTTRGPVLTAAARHGYRIVLWSDMVQRAAHRADQDVARLLATA
                     APGQVLLAHDGRGDRLGTLQRLPLLLAGLTAAGYTLTTVGELLASG"
     gene            complement(4904..6160)
                     /locus_tag="VEP73_06890"
     CDS             complement(4904..6160)
                     /locus_tag="VEP73_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT26192.1"
                     /translation="MRPPGALDRLPEWMRSIRFRYTLLYSGVLFGLAVLLLGALYLIL
                     SITLHGETPTAQTLCINFGTCIDFFTGRQFQQLVHARTLEKLRMFSFDALVVLFVASL
                     GVGWIIAGQVLAPIGRITAVARDIQATDLSRRIELHGPDDELKQLADTFDAMLARLDA
                     AFAAQQQFLADASHELRNPLAIIRTNVDVALADARADPEDLRHAMTVVKRASDRMARL
                     VDDLLALASRQAPTLSHEAVALGVAIAEASEEFVAPAEMRGITLDRAITPGIVVLGDR
                     DALKRAVANLLENAVRLAPPGSRIRLAVGSERGMAWVAVSDEGPGIRPEDQAKVFDRF
                     WRADKARSRADGGTGLGLAIVRQIAQSHGGEVQLQSRVGVGSTFTIWLPVVSADEPAR
                     VVPPGVPARAQRVAIEQRRLDPPRRP"
     gene            complement(6157..>6825)
                     /locus_tag="VEP73_06895"
     CDS             complement(6157..>6825)
                     /locus_tag="VEP73_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26193.1"
                     /translation="VEDEADLADAVARGLRREGYAVDVAYDGDEALEKAAVNAYDVVC
                     LDLNLPGTDGIEVVRQLRSTPDSRPVPRVLMLTARDRPVDRVAGLDEGADDYLVKPFD
                     FDELKARIRALLRRDAGRSGALLRAGDLELDAARHQAFREGRPLQLTPKEFALLRYFM
                     SRAGQVLSQEDLLEHVWDEHADPFTNTVRVTVMTLRRKLADAGDGQPIETVVGRGYRL
                     REDA"
BASE COUNT          839 a         2528 c         2611 g          847 t
ORIGIN      
        1 gcgatgagct gccggtcccc cgccccgagg cggtagggga cgccgtagcg ctcgagcgcc
       61 cgccggatcg gcccgctcgc tcgccgtacc gaaggcagga gcaccgccat ccgcccatag
      121 ggaacgccgt cgcgctggtg ggccacccgc agcagccgcg ccagtgcgtc ggcctcgtcg
      181 gccggcccaa ggtggcgggc gacctccacg gcggtcgtcc gcccgccacc gagcgccacc
      241 ggcggctcgt ggccgccacg ctggaggcgc tggacggcgt cgaggccgtg cccgccgagc
      301 cgccggctgt gctcgaggcg tacgacggcg gcccccagcc gcccggccca gccgggcagc
      361 gcgcccgggg cggcgccccg gaaggcgtcc gtggtcccgg ccgggtcgcc ggccagcacg
      421 gcgcgggcgc cgccgccgac cagcagctcg aggagccgcg actgggccgg gtcgagctcc
      481 tgcgcctcgt cgacgagcac cgttccggtc cgggcgaccg tcgtggccag cacctccggc
      541 tggtcctcga gcaggtcgcc ggcctgggcc accagtccgg cctggtcgac cgagtcctcc
      601 agggcgaggc ggtcggcata cgccgagagg aacgcggcgc cgggcaccca cgccgggcgg
      661 ccgccggccc cgaccctggc gagcaactcg tcgggcccga tgaggcgacg ctcgcacgcg
      721 agcacgaagt cggccagctc gtcggtgaac gcgcgcgtgc ccaccagcgg ggcgaggtcc
      781 ccccagtcga cgccggccgt ctccaggagc agcgaccgca cgagcgacca ctgctcgggc
      841 ccggagagca ggcgcggctc gccccggtag gagagcaggt cggcgtggct ggtcacgagc
      901 ccacgggcga aggcgtgcca ggtgacgcag ccgagcggcc cggtcgccgc cggcggcgac
      961 gcggcgagca gccgttcgct ggaggcgacc gccgcctgcc tgctcgacgt gaggaacgcg
     1021 gcaccgcccg cctggccggc cacgagcgcg ggcatcatcg cctcgagcag cgaggtcttg
     1081 ccgctgccgg gcccgccgag cacgacgacg ttgccgggcg ggagcatggc ggcgaggtcg
     1141 ccgggcaggg cgtcggggag cacggtctca gtggtcatgc ccgcatccta gacgcgagcc
     1201 gcgacggcgc gggcggccgt ccacagggtc ggccgcgagc ggtcgcgccc gaccggccgc
     1261 cggggctgtg gacggccgct cgcgggcgcc ggccatggcg gttacggtcc ctcagccgcc
     1321 ggcgaccggc cgcgcggccc gggacagcgg cccggcccca ccggaggagg cagaccatcg
     1381 gaccccgccc gcccggcgcc cgcccggcgc ccgccgccgt cctgctcgcc gccgtgctcg
     1441 ccgccgtcgt gctcggcggc tgttcaccca gcacccgggc gccggcgccg ctggccaccc
     1501 cggcggccga gcgcaggtcg tggcctgggc cgccgccggg cgcggccacc gccctcgtcg
     1561 agcgggtggt cgacggcgac accttcgtgg cggtggtcgg cggccggcgg gaccgcgtca
     1621 gggtgatcgg cgtcgacacg cccgagacgg tcgcccctgg ccggccggtc gagcggtacg
     1681 gcaggcaggc gtcggcattc gccaagcact ggctcaccgg ggccacggtc cgcctggccg
     1741 gcgacgtcga gtcacgggac cgctacggcc gtctgctcgc ctacgtgtgg ctggcggacg
     1801 ggaccttctg gaacgcgctg ctcgccgccg agggatacgg gcagcagctc acgattccac
     1861 ccaacctcgc gttcgcggag ctgctcgggc ggctggtcgc cgaggcgcgg tcccaccacc
     1921 ggggcctgtg ggcggctggc gggcgcgtca cgcgaggtga gagcggcttg cgcgcatgag
     1981 cggaaccgcc atcgatcggg tagtgtaccg gcgcctggag agggcaacgg cctgccgtcc
     2041 ggcggggccc tggcagtccc ccaaccacca gtcctcacag ccggacggcg aggccgccgc
     2101 aacgcgaacc tgcttcccgc gcctcccgtg tcctcccggc caaggcgtga cggccccggc
     2161 cgtcccagcg ggcggaagtg gatcgcacgt gcgcccccca gcgccgcctc gatcgccgtt
     2221 ccgtgttcgc gttactgaca gtacgatgcc attcccgttc gtgccgagcc cgagtccaaa
     2281 tcgttaccag gcaaactacc agagccggcc gaacctgtct acgggtcttc tgcagctggc
     2341 taggtatccc ttctcatcgg atcggacgct cggtgagcgc ggccgtgacg gtctgctcct
     2401 tcccacctcg gaccagcttc acacccacct tctggccggg cgcggccaag cgcacctggg
     2461 cggccaggtc gtcgctcgac tggatcgcct tgccgttgaa ctcgatcacc aggtcgccga
     2521 cctggatgcc ggccttctcg gcgggcgagc cgggcaggac ctggtcgatg agcgcgccgc
     2581 cccgcccgag cgtcggggtg ctcaggctga ccccgaggta cccgctctgg atcttctcgc
     2641 ccttgacgat ccgggaggcc gagctggcgg cggtgtcgat cgggatcgcg aagccgacgc
     2701 cctcgttccc gccggactcg gagcggatcg agtcgttgat gccgatcacc cggccgctcc
     2761 ggtcggcgag ggcaccgccg gagttgcccg ggttgatcgg cgcgtcggtc tggatgacct
     2821 cgagcgcgcc gccgttgccc gtcgccaggc tgcggttctt ggcgctgacc acgccggccg
     2881 tgaccgtctg ctgcagcccg aaggggctgc cgatggcgac ggcggtctgg ccgacctgca
     2941 cgtcgacccc gagggcgagg gcggcgggct tcaggccgcc gcggtcgacc ttgaccacgg
     3001 cgatgtcgtt gccctggtcg atgccgagca cctggccgcg cagcgtgctg ccgtcgtaga
     3061 gccggacggt gaccgtgccc gagccctcga cgacgtgggc ggcggtcagg acgtagccgt
     3121 tcctgtcgta gatgacgccg gagccgagac cctggtcggt ctcgatctgg acgacgctgg
     3181 ggccgagcgt cctggcgacc gcggcgaccg gctcctcgcc ggcgggcagc ggcgcgggct
     3241 gggcggccac cgggaccccg gccccggccg ggacgcccgc gctcgagtcg cgacggtcga
     3301 cgagcagggt gagcccgacg cccgtgagga ccaggagcag cgcggcgatg gtcgcggcga
     3361 gcgcccggcc cgcgccgcgg cggggccgag gagcgacgcc ggagccgggt gggggaccgc
     3421 cccagccggg cacgcccccg ctcggcgacg cccccccgac ggcggtcgac gtccaggccg
     3481 gccggtcgcc cggcacctcg ccggaggccg cgtgctgggc gcccggcgac gtggcgccac
     3541 cccaggtggg cgcgtcggag gcgggggcgc cccaggtggg cgcgtccgag tccggagcat
     3601 cccaggtggg cgcgtcggag gcgggggcgc cccaggctgg ggcgcccgag ctggggccat
     3661 cccaggtggg cgcgtcccgc gaggggccgg cgtgctggcc ccagccgttg cctccgttga
     3721 cgggcggcgc ccaggtgggg acggtccggg ccgggtcagg gtcagggcgg accgggcggg
     3781 tctcggcgtc ggggtcccgc aacggtctgg tctcgttcgg gtcgttcttg ggggtggcca
     3841 tcgagggtcc tcctggacga gcactgtcga gcttccaggc caggataggc agccagcccc
     3901 taaagcatcg gtaagccaga cgtcaaggca ggctaaagct gacaccgctg aggcggtgac
     3961 gggccgccgc gcgctcctct ggcgctcacg cccgtggcgc tcatgcccgg cgcccgggcc
     4021 tgtggctctc atgccggtct cactccccgt gcggcagcct ccgaaggtcg tcgtgccgtg
     4081 gcggaagcga ggggacgtgg gccaggcgga acggccaggg gcagcacgcg accagggcag
     4141 gcgcgggttc cttcgcgggg cggtcgcgac cgggctggca gctgccatcc tggcgacgag
     4201 cgggcgggag gccgaggctc gccacgccac cgggcctggc gacctcgccg cggcgacggt
     4261 gccgccgtgg cccgagccat accttggcca gctctcgatc acctggaggg tggcgaccgg
     4321 ccggcgggcg gtcgccatct ccttcgacga cggccccgac ccacgctgga ccccggccgt
     4381 gctcgacgcc ctggcgcacg cgggcgcgag ggccaccttc ttctgctgcg ggcaggccgc
     4441 ccgccgccat cccggtctgg tccgccgggc cgccgcggcg ggagagatcg gcaaccactc
     4501 ctggagccat cccgacctgg cggcgctcag ctccctgcag gtcgaccgcg agatcgaccg
     4561 gacccacgac ctgctcggcg ggatcctcgg ccgcccgccc acgctgctcc ggccaccgta
     4621 cggcacgacc cggggtccgg tgctgaccgc cgcggccagg cacggctacc ggatcgtgct
     4681 ctggtcggac atggtgcagc gggccgccca ccgggccgac caggacgttg cgcggctgct
     4741 cgcgaccgcg gcgcccggtc aggtgctcct cgcccacgac ggccgtggcg accggctcgg
     4801 cacgctccag cgcctcccgc tcctcctcgc cgggctgacc gcggccggct acaccctcac
     4861 gacagtcggt gagctgctgg cttcaggctg accgccggtg gcgctacggc cgcctggggg
     4921 gatcgagccg ccgctgctca atggccaccc gctgggccct ggccgggacg ccgggcggga
     4981 cgaccctggc gggctcgtcg gcggagacga ccggcagcca gatggtgaag gtggagccga
     5041 ccccgacccg ggactggagc tgcacctccc cgccgtggct ctgggcgatc tggcggacga
     5101 tggcgaggcc gaggccggtc ccgccgtcgg cccgcgagcg ggccttgtcg gcccgccaga
     5161 accggtcgaa caccttggcc tggtcctcgg gccggatccc cggcccctcg tcggacacgg
     5221 cgacccacgc catgccccgc tcactgccca cggccaggcg gatccgggac ccggggggag
     5281 cgagccggac ggcgttctcg agcaggttgg ccactgcccg cttgagcgcg tcccggtctc
     5341 cgagcacgac gatccccggc gtgatggccc ggtcgagcgt gatcccgcgc atctcggcgg
     5401 gggcgacgaa ctcctcgctc gcctcggcga tcgccacgcc cagggcgacg gcctcgtggc
     5461 tgagggtcgg ggcctggcgg ctggccagcg cgagcaggtc gtcgacgaga cgggccatgc
     5521 ggtcgctcgc ccgtttcacc acggtcatgg catgacgcag gtcctccggg tcggcgcgcg
     5581 cgtcggccag tgccacgtcg acgttggtgc ggatgatcgc gagcgggttg cgcagctcgt
     5641 gggaggcgtc ggcgaggaac tgctgctggg cggcgaacgc ggcgtcgagg cgggcgagca
     5701 tcgcgtcgaa ggtgtcggcg agctgcttca gctcgtcgtc ggggccgtgc agctcgatgc
     5761 gccgggacag gtcggtggcc tggatgtccc tcgccaccgc ggtgatgcgg ccgatcggcg
     5821 cgaggacctg gccggcgatg atccagccga cgccgaggct cgcgacgaac agcacgacga
     5881 gggcgtcgaa cgagaacatc cgcagcttct cgagcgtccg ggcgtgcacg agctgctgga
     5941 actggcggcc ggtgaagaag tcgatgcacg tcccgaagtt gatgcacaac gtctgggcgg
     6001 tcggggtctc gccgtgcagc gttatcgaca ggatcaggta gagcgcgccg aggagcagga
     6061 cggccaggcc gaacaggacc cccgagtaga gcagggtgta gcggaaccgg atggaccgca
     6121 tccactccgg cagccggtcg agggcgccgg gcggtctcat gcgtcctcgc gcaggcggta
     6181 gccgcgcccg accaccgtct cgatgggctg gccgtcgccc gcgtcggcga gcttgcggcg
     6241 cagggtcatc acggtcaccc gcacggtgtt ggtgaacggg tcggcgtgct cgtcccagac
     6301 gtgctcgagc aggtcctcct gggacagcac ctggccggcc cgggacatga agtagcgcag
     6361 cagggcgaac tccttcgggg tgagctgaag ggggcgcccc tcgcggaacg cctggtgccg
     6421 ggctgcgtcg agctccaggt cgcccgcgcg caggagcgcg ccggagcgcc cggcgtcgcg
     6481 cctgagcagc gcacggatgc gcgccttgag ctcgtcgaag tcgaagggct tgacgaggta
     6541 gtcgtcggcg ccctcgtcga ggccggcgac ccggtcgacg ggccggtccc gcgcggtcag
     6601 catgagcacg cggggcacgg gcctcgagtc gggggtggag cggagctgcc gcacgacctc
     6661 gatgccgtcg gtccctggca ggttcaggtc gaggcagacg acgtcgtagg cgttgacggc
     6721 cgccttctcg agggcctcgt ccccgtcgta ggcgacgtcc accgcgtagc cttcccgccg
     6781 caggccgcgg gcgaccgcat cggccaggtc ggcctcgtcc tccac
//
LOCUS       DASPYY010000441         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1902538, whole genome shotgun sequence.
ACCESSION   DASPYY010000441 DASPYY010000000
VERSION     DASPYY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902538"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VEP73_06900"
     CDS             complement(<1..1537)
                     /locus_tag="VEP73_06900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017606406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT26194.1"
                     /translation="MTTPRSDAAQTQTVRDDLLPGLRGWQKDAYHEYFRLPRRDFLLV
                     ATPGAGKTAYALTVAAELLARREVAFLTIVTPTEHLKHQWAAAAARFGIAIDSAYRNA
                     QGRAGADFAGVAVTYAQVAAHPALHRQRTENRRTLVIFDEIHHAGDALSWGDAVKEAF
                     DPARRRLGLTGTPFRSDANPIPFVTYLDEPGGGKRSASDYVYGYGPALEDGVVRPVIF
                     LAYSGEMRWRTRAGDEITATLGTPMTKDQIAQAWRTALDPAGEWVSRVIEAADKRLTE
                     VRRGMPDAGGLVIAGDHEDARAYAALLRGLTGKRPVVVLSDDKTASKKISAFAGSGDR
                     WMVAVRMVSEGVDIPRLAVGVYATSVSTALFFAQAVGRFVRARQRGETASVFLPSVPV
                     LLSYAAELEAERDHVLRAPGAAGEEDELALAEALRERDTPDAPLDEPAFTALNASAHF
                     DRVLYDGGEFGTATAAGSPEEEDFLGLPGLLDPDQVRTLLRKRQDAQLAAPASPAPAS
                     PARV"
BASE COUNT          223 a          603 c          643 g          224 t
ORIGIN      
        1 cgacgcgggc ggggctggcg ggggcggggc tggcgggggc ggctagctgg gcgtcctggc
       61 gcttgcggag cagggtgcgg acctggtccg ggtcgagcag gccgggcagg ccgaggaagt
      121 cctcttcctc tggcgagcca gccgccgtgg cggtgccgaa ctcgccgccg tcgtacagca
      181 cccggtcgaa gtgcgcggac gcgttgaggg cggtaaaggc gggctcgtcg agcggggcgt
      241 ccggggtgtc gcgctcgcgc agcgcctcgg ccagcgcgag ctcgtcctcc tcgccggccg
      301 cgccgggggc gcgcagcacg tggtcgcgtt cggcctcgag ctcggcggcg taggacagca
      361 gcacgggcac cgacgggagg aacaccgacg cggtctcgcc gcgctgccgg gcccggacga
      421 accggccgac cgcctgggcg aagaacagcg cggtggacac gctggtcgcg tacaccccga
      481 cggcgaggcg cgggatgtcc acgccctcgg acaccatccg caccgcgacc atccaccggt
      541 caccggaccc ggcgaacgcg gagatcttct tgctcgcggt cttgtcgtcg gagagcacga
      601 cgaccgggcg cttgccggtg agcccgcgca gcagcgcagc gtacgcccgg gcgtcctcgt
      661 ggtcgcccgc gatgaccagg ccgcccgcgt cgggcatgcc gcggcggacc tccgtcagcc
      721 gcttgtcggc cgcctcgatc acccggctga cccactcccc cgccgggtcc agggcggtcc
      781 gccaggcctg cgcgatctgg tccttggtca tcggcgtgcc cagggtggcg gtgatctcgt
      841 cgccggcccg ggtgcgccag cgcatctcgc ccgaataggc cagaaagatc accggccgga
      901 ccacgccgtc ctcgagcgcg ggaccgtagc cgtagacgta gtccgaggcg ctgcgcttgc
      961 cgccgcccgg ctcgtcgagg taggtgacga acgggatcgg attggcgtcg ctgcggaacg
     1021 gcgtgccagt cagcccgagc ctgcgccgcg ccgggtcgaa tgcctccttg accgcgtccc
     1081 cccaggacag cgcgtcgccc gcgtggtgga tctcgtcgaa gatgaccagc gtgcgccggt
     1141 tctcggtgcg ctgcctgtgc agcgccgggt gcgccgcgac ctgggcgtag gtgacggcca
     1201 ccccggcgaa gtcggcgccg gcccggccct gggcgttgcg gtacgccgag tcgatcgcga
     1261 tgccgaacct ggcggcggcg gcggcccact ggtgcttgag atgctcggtc ggcgtgacga
     1321 tggtgaggaa cgcgacctcg cgccgggcca gcagctcggc cgccaccgtc agcgcgtagg
     1381 ccgtcttgcc cgcgccgggc gtggcgacca gcaggaagtc gcgtctcggc agccggaagt
     1441 actcgtggta ggcatccttc tgccagccgc gaaggccagg aaggagatca tcccgaacgg
     1501 tctgggtttg cgcagcgtca gatcgagggg tggtcacgag gccgaaatgc taccggcgcg
     1561 aaccgccccg cgctggcaaa ggcacgccgg ggtggcagtg ttggcccccg ggggccgacc
     1621 ccccggaccc cccgaaaaca aggagtcact cgccgccgtc gtcgcccggg gggagcccct
     1681 cgtagatctc ctt
//
LOCUS       DASPYY010000442         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1760324, whole genome shotgun sequence.
ACCESSION   DASPYY010000442 DASPYY010000000
VERSION     DASPYY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760324"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..821
                     /locus_tag="VEP73_06905"
     CDS             327..821
                     /locus_tag="VEP73_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYT26195.1"
                     /translation="MAQAPFPDRGPAEPAPEPWEHGLGRLRHVVAGVDGSTASFGAAR
                     RAAELAVMAGARLTVVHVRPRAPVFSAFAALTEAAVQQWREDVEWEVVVELTTLLDPL
                     GICWRLQVEEGDPARRLDEVTRALGADLLVVGTSRRRGPRLHRSVAYRVVRNAHTPVL
                     VVHA"
     gene            complement(841..2172)
                     /locus_tag="VEP73_06910"
     CDS             complement(841..2172)
                     /locus_tag="VEP73_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HYT26196.1"
                     /translation="MEPGPHDVEPAGPSERAPEPARVDRRRFLAGALAGGAVATAAGY
                     GLGLRARGGSGTGDRLGPGPAGPAPEAGTWSVFGPHQPGITTPQPASGLVAAFDVDAR
                     SRAGLGDTLELLTAKSQDLMSGASPPARDAAVPPPDSGVLGPGWSPDGLTVTVSVGAS
                     LFDHRYGLAALRPRRLVRMPAFPNDDLDRSRCHGDLAIQLCAAHRDTVLHALRELRGA
                     TARMLTQRWMLEGFQRPNTLGPGRTSVRNLLGFKDGTANLDPRDGELMGRLVWVPPGG
                     TEPAWAAGGSYQVVRIIKLFVEKWDLTPLRVQEEVIGRHRDSGAPLGGRREDEPPDFA
                     SDPHGQVTPPFAHIRLANPRTAATEDSRILRRGFNYARDAAGPGVDTGLLFISYQRDL
                     ERGFATVQRRLAGEQLEEFVQPVGGGYFYALPGASAPGGYLGEPLIAAAGA"
     gene            2392..>2588
                     /locus_tag="VEP73_06915"
     CDS             2392..>2588
                     /locus_tag="VEP73_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26197.1"
                     /translation="MGSIIAGAFMQASTFVAPRTAPVHTSTGAQGGAGAWLPLLLAVL
                     VTGLLLATVLSPWRRRSGRRLL"
BASE COUNT          324 a          957 c          977 g          330 t
ORIGIN      
        1 gagcgggtcg aggtggatgc cgcggaagat cgcgggcagg gagatgatca cgatcgacga
       61 gttcatcgtg gccacgagga tcccgagcgt cgtgttcgac agcgcgaccc agggagaagg
      121 ccaggccggc gcgctgggag cgggcctggc gtgcgcgtct cccatcggtc acgacccctt
      181 cctcggcatg agaacccatc gacggcctga aaacctgacc tttacgttaa gatcatagag
      241 aaggatccgc tacccggcaa cgcggtggta cacggcctcg tgcgctgggt agactccgag
      301 tcacgcagcg agcggaagag ggttccatgg cgcaggcgcc gttcccggac cgggggccgg
      361 cggagccggc acccgaaccg tgggagcacg gccttggccg cctccgccac gtcgtggccg
      421 gggtcgacgg gtcgaccgcc agcttcggcg ccgcccgccg cgccgcggag ctcgcggtca
      481 tggccggcgc ccgcctgacc gtcgtacacg tccgcccacg cgccccggtc ttctcggcgt
      541 tcgccgccct caccgaagcc gcggtccagc agtggcggga ggacgtggag tgggaggtcg
      601 tcgtcgagct cacgacgctg ctcgacccgc tcggcatctg ctggcgcctc caggtcgagg
      661 agggcgaccc cgccaggcgc ctggacgagg tcacccgggc gctcggcgcc gacctgctgg
      721 tcgtcgggac gagccgccgc cgcggcccgc gcctccaccg gtcggtcgcc taccgggtcg
      781 tccgcaacgc ccacacgccg gtgctcgtgg tccacgcctg agcaggtccc gccgggcgcg
      841 tcaggcgccg gcggcggcga tcaggggctc gccgaggtag ccgccggggg cgctcgcgcc
      901 ggggagcgcg tagaagtagc ctccgccgac cggctggacg aactcctcga gctgctcccc
      961 ggccaggcgg cgctggaccg tcgcgaagcc gcgctcgagg tcccgctggt agctgatgaa
     1021 cagcaggccg gtgtcgacgc cgggcccggc ggcgtcgcgg gcgtagttga agccgcgccg
     1081 caggatgcgc gagtcctcgg ttgccgcggt ccgcgggttg gccaggcgga tgtgcgcgaa
     1141 cggcggcgtg acctggccgt gcgggtcgct cgcgaagtcg ggcggctcgt cctcgcgccg
     1201 cccgccgagc ggcgcgccgc tgtcccggtg gcgcccgatg acctcctcct ggacccgcag
     1261 cggcgtgagg tcccacttct cgacgaacag cttgatgatc cggacgacct ggtagctccc
     1321 gccggccgcc cacgccggct cggtcccgcc gggcggcacc cacacgagcc ggcccatcag
     1381 ctccccgtcg cgcgggtcca ggttcgccgt gccgtccttg aagccgagca ggttgcggac
     1441 cgaggtacgg ccgggaccga gcgtgttcgg ccgctggaac ccctcgagca tccagcgctg
     1501 cgtcagcatg cgcgcggtcg ccccccgcag ctcgcgcagc gcgtgcagga ccgtgtcgcg
     1561 gtgggcggcg cagagctgga tcgccaggtc gccgtggcac cgggaccggt ccaggtcgtc
     1621 gttcgggaag gcgggcatcc gcacgagccg cctcggccgc agcgcggcga gcccatatct
     1681 atggtcgaac agcgaggcgc cgaccgacac ggtgaccgtc aggccgtcgg gcgaccagcc
     1741 ggggccgagg acaccggaat ccggcggcgg cacggcggcg tccctggccg gagggctcgc
     1801 accgctcatg aggtcctgcg acttggcggt gaggagctcg agggtgtccc cgagcccggc
     1861 gcggctcctg gcgtcgacgt cgaatgcggc gaccagcccc gacgccggct gcggggtcgt
     1921 gatgcccggc tggtggggcc cgaagaccga ccaggtgcct gcctccggag cgggtccggc
     1981 ggggcccggg ccgaggcggt caccggtccc cgagccgccc cgggcccgca ggccgagccc
     2041 gtagccggcg gcggtcgcga ccgcgccccc cgcgagcgcg ccggccagga accgccgccg
     2101 gtcgaccctt gccggctcgg gcgcccgctc ggacgggccg gccggctcga cgtcgtgggg
     2161 tccgggctcc atcgcggcga cctctgcctc cgaaggaccg acgctgcgcg ccccgggccg
     2221 gtacggggct ggcgccagta tggccagtcc ccgatccccc tcacaagatc acggaagttc
     2281 ccggaccggg cgttcccggt tcggtatcgc gggtacacaa ggccgtgtgc acgtcgcgcc
     2341 ggggcccggc gccaggacgg cggaacggga aagcgaccag tgagcggcat cttgggttcg
     2401 atcatcgcgg gcgcgttcat gcaggcctcc accttcgtcg ctccccgcac ggcaccggtg
     2461 cacacctcca ccggagcgca gggcggcgcc ggggcgtggc tgccgctcct gctggcggtg
     2521 ctcgtgacag ggcttctcct ggcgaccgtc ctctcgccct ggcggcggcg gtcgggccgc
     2581 cggctgct
//
LOCUS       DASPYY010000443         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1058213, whole genome shotgun sequence.
ACCESSION   DASPYY010000443 DASPYY010000000
VERSION     DASPYY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058213"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VEP73_06920"
     CDS             <1..859
                     /locus_tag="VEP73_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26198.1"
                     /translation="DFVRALRAELGEPSMTAAATAAEEAADEAAFYGDGQEMAGQAAG
                     DEEALVPAEPGSNGWPLPDADGQEAPAPRRRRLGLPLLLGAILLLAAGAAGYLLLRGG
                     DAPKSFSANDVAPFSFSYPAAWKVNEHSNLFVSFAPYDATELFADSNWRGTNTALRGN
                     ARSVWGMYTRVKTAGFDIQQAPDDLDRELQAALPGIVTVTDHVPGVVRVDGFPAAEIE
                     GEVTDQGSQGRLHFLYLIVQVDPRGPQTVHMVFFSSPDAFDDARRAEFQRVKDSVRFD
                     RERLKILHS"
     gene            complement(863..3157)
                     /locus_tag="VEP73_06925"
     CDS             complement(863..3157)
                     /locus_tag="VEP73_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT26199.1"
                     /translation="MSDLVGRELGQYQLRQLIRRGGMATVYKAFQPSLERWVAVKVLT
                     NPGDPTFVERFRREARSIARLQHPNIVPIHDYGEADGQPYLVVSYVEDGHSLAGDVGT
                     PMAPARALELCTHLLAGLGYAHEYGVVHRDVKPANVLLPAPDWPMLADFGIAKLVLGD
                     GTALTQTGLVIGTPAYMAPEQAFGLPVDARTDLYAVGLVLYELLTGRVPFEADTPTTA
                     LMKQAYEPPPSPRTLNPDLPVEVERLLLRALAKDPGERFQDAASMAQAVDAARGALPV
                     EAPDGSAAGSFAAAYAEGVAAYSAGRWQEAIDRLTTLAELDPGYEDVEELLDTARAAR
                     ERAAARGPVPDPGLGSPAAVASVAAASPAERAPSLAADAPLPPPAPVPAVSVPQAGPP
                     PVPGAAPTPVPGAGPVAAPTPPSPPAAPRGRRSRPRGWLLAGVAALVGVAALVAVVLS
                     LGRDGTRTAARSASAATEPTTATSAPAQAWTTLPRAPLALESSGTAAFQGRVWVAGGF
                     DEAHKGRADVLVYDPVGATWSRGPGLPRPLTHAALVSTGRELYLIGGYRGSTPVPLTT
                     VRRLDPAAGRWVRGPDLPVGLGAGAAAWDGRRVVFAGGVGGDGRPSAAVYALGNGSWR
                     RVGTLPKAREHLAAASDGKGTTFFLAGEVNGPGGKVVLAEVDAVSGGTVRRLGDVPQP
                     RGSVAGFWSPAAGACVAGGRDGSGNLFSEVDCVDAKGTVTRLPSLPTPRHGLGIAVVD
                     GTAYAVLGAAPTNVRTAEALRLEP"
     gene            complement(3413..3901)
                     /locus_tag="VEP73_06930"
     CDS             complement(3413..3901)
                     /locus_tag="VEP73_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26200.1"
                     /translation="MSEDPTQELPFPGQRPSGRAPRQPRSGKVWRSVLALALAASFAV
                     ATAAVVMAQAHKPAPVAGPVALAGQTAPTTNQTAERAAILPVPSSNPGKVAQCAARDA
                     RERADDVRDAAEEAREATETPAQKLARKRAEDAQKAREDAREAAEDRLCGEYHDPGDV
                     DD"
     gene            complement(4038..5579)
                     /locus_tag="VEP73_06935"
     CDS             complement(4038..5579)
                     /locus_tag="VEP73_06935"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HYT26201.1"
                     /translation="MTPALTADAGLRAGSVRFRPSEGELAELTDSMPAARRTRFGNVN
                     VRTRVQDRLRSATFVVDDRPDRHPGQPMGRAEGALIAAVQDAYIESHDMVVVDGFIGH
                     AGPHRTLARLVVERANAGIAGMQRHLYYDPVVGDAPVAPELTVICTPNLGVPGYPDGR
                     VVAVWPEEGVTRIVGSDYFGEAKKAVLRMWSWRFHDAGGLVMHAACKVVPTARGPRTM
                     LIVGRSGTGKSTTTFTCHNGSRVVQDDFVGLLPGGRIVPSEDGCIEKTYGLDPLLQPL
                     VHAAATGPDAYLENVSQQGEELDFLDPTSTRHGRAVFNLRSVDRFPPDRVPPASILVI
                     LNRDEHVTPAVARIDPSQAARHYLLREIHGPAADGRQESGGRRGSDPLALEYARHGNR
                     LAELLRDHPMEVFLVNTGRVGGPARAGRSKAIAVSDTSAVVAAVAEGTIAWDGDDPDL
                     GCEVAADVPGIDDRELYRPSLLYRRQGRLAEYRSRAAHRAATAAWYLAGLPGLDPEVS
                     GGSGT"
BASE COUNT          736 a         2256 c         2279 g          769 t
ORIGIN      
        1 cgacttcgtg cgcgcccttc gcgccgagct gggggagccg tccatgacgg cggcggccac
       61 cgcggcggag gaggccgccg atgaggcggc gttctacggc gacggccagg agatggccgg
      121 ccaggccgcc ggcgacgagg aggcgctggt ccccgcggag ccaggatcca acggctggcc
      181 gctcccggac gccgacgggc aggaggctcc cgcgccgagg cggcgccgcc tcggcctgcc
      241 cctgctcctc ggcgccatcc tgttgctcgc cgccggggcc gccggctacc tgctgctgcg
      301 gggcggcgac gcgcccaagt cgttcagcgc caacgacgtc gcgccgttct cgttcagcta
      361 cccggcggcc tggaaggtga acgagcattc caacttgttc gtctcgttcg cgccctacga
      421 tgccacggag ctgttcgccg acagcaactg gcgcggcacg aacaccgccc tgcgcgggaa
      481 cgcccgcagc gtgtggggca tgtacacgcg ggtcaagacc gccggcttcg acatccagca
      541 ggcgcccgac gacctcgacc gggagctgca ggccgccctg cccgggatcg tgaccgtcac
      601 cgaccacgtg cccggggtcg tccgggtgga cggctttccc gccgccgaga tcgagggcga
      661 ggtgaccgac cagggcagcc agggcaggct ccacttcctg tacctgatcg tccaggtcga
      721 cccgcgcggg ccgcagaccg tccacatggt cttcttctcg tccccggacg ccttcgacga
      781 cgcccgccgg gccgagttcc agcgtgtcaa ggactcggtg cgcttcgacc gggagcgcct
      841 caagatcctg cactcgtagc gctcagggtt cgagccgcag ggcctcggcg gtacggacgt
      901 tcgtcggcgc cgcaccaagg acggcgtacg ccgtgccgtc gacgaccgcg atccccaggc
      961 cgtgccgcgg cgtcggcagc gacggcaggc gcgtcacggt gcccttggcg tcgacgcagt
     1021 ccacctcgga gaacaggttc ccgctcccgt cccggcctcc ggcgacgcag gctccggcag
     1081 ccggcgacca gaagccggcg accgagccgc gcggctgggg gacgtctccc agccgccgca
     1141 cggtgccgcc gctgacggcg tcgacctcgg ccagcacgac cttcccccca ggcccgttga
     1201 cctcgccggc gaggaagaac gtggtgccct tcccgtcgct ggccgcggcc aggtgctccc
     1261 gcgccttcgg cagggtgccg acccggcgcc acgacccgtt cccgagggcg tagacggcgg
     1321 cggacggcct gccgtcgccg ccgacgccgc cggcgaagac cacgcgccgt ccgtcccatg
     1381 ccgcggcgcc cgcgccgagg ccgaccggca ggtccggccc cctgacccac ctcccggccg
     1441 ccgggtcgag ccgccgcacg gtcgtcaggg ggaccggcgt ggagccccgg tagccgccga
     1501 tgaggtagag ctccctgccc gtcgacacca gcgcggcgtg ggtgagcggg cgcggcagcc
     1561 cgggccccct gctccaggtc gcgccgaccg ggtcgtacac cagcacgtcg gctcgcccct
     1621 tgtgtgcctc gtcgaagcct cccgccaccc agacccgtcc ctggaacgcc gcggtcccgc
     1681 tcgactcgag cgccagcggc gcccgcggca gcgtggtcca cgcctgggcc ggggcgctgg
     1741 tcgccgtcgt cggctcggtc gccgccgagg cgctgcgggc cgccgtgcgg gtgccgtcac
     1801 gaccgagcga caggacgacg gcgaccagcg cggcgacgcc gaccagggcc gccacgcccg
     1861 cgagcagcca gccgcgcggc ctggagcgcc gtccccgggg tgcggccggc gggctgggcg
     1921 gcgtcggcgc ggccaccgga ccggcccccg ggacgggcgt cggcgcggcc cccgggacgg
     1981 gcggcggacc ggcctgcggg acggagacgg cggggaccgg cgccggggga gggagcggcg
     2041 cgtcggcggc gagggacggc gcccgctcgg ccggcgaggc ggcggcgacg ctcgcgaccg
     2101 ccgcgggcga cccgaggccc gggtcgggaa ccggtccgcg cgctgcggcg cgctcgcggg
     2161 ccgcgcgggc ggtgtcgagc agctcctcga cgtcctcgta gccggggtcg agctcggcaa
     2221 gcgtggtgag acggtcgatg gcctcctgcc agcggccggc gctgtaggcg gccaccccct
     2281 cggcatacgc agccgcgaac gagccggccg ccgaaccgtc gggggcctcg accggcagcg
     2341 cgccacgggc ggcgtcgacc gcctgggcca tcgacgccgc gtcctggaag cgctcgccgg
     2401 gatccttggc gagcgccctc agcagcaggc gctcgacctc gaccggcagg tcggggttga
     2461 gcgtccgggg cgagggcggc ggctcgtagg cctgcttcat gagggcggtc gtcggggtgt
     2521 cggcctcgaa cggcacccgg ccggtcaaca gctcgtagag caccaggccg accgcgtaca
     2581 ggtcggtgcg ggcgtcgacg gggaggccga aggcctgctc gggggccatg taggcggggg
     2641 tgccgatgac caggcccgtc tgggtgaggg cggtcccgtc gccgagcacg agcttggcga
     2701 tgccgaagtc ggccagcatc ggccagtccg gggcgggcag cagcacgttg gcgggcttga
     2761 cgtcgcggtg caccacgccg tactcgtggg cgtagccgag gccggcgagc aggtgcgtgc
     2821 acagctccag cgcccgggcg ggggccatcg gcgtgccgac gtcgccggcg aggctgtggc
     2881 cgtcctccac gtaggagacc acgaggtacg gctggccgtc ggcctcgccg tagtcgtgga
     2941 tggggacgat gttggggtgc tgcagccgcg cgatcgagcg ggcctcgcgc cggaagcgct
     3001 ccacgaacgt cgggtcgccc gggttggtga gcaccttgac ggccacccag cgctccagcg
     3061 acggctggaa ggccttgtag acggttgcca tgcctccccg tcggatcagc tggcggagct
     3121 ggtactggcc gagctcgcgg ccgacgaggt cgctcatggt cgcccttccc gggagcccgg
     3181 ccctcgcccg ccccccgatg ccgttcccca cgcctccaag cggaggccgc cgactagtat
     3241 cgcccagcgc ccgtcccagg tcagccgcct tgacgtcggc catccctccg ccctccgaat
     3301 gctcgggcgc ccggcctccg gtcgccaccc accacggcga agaccgctgg tcaggagctg
     3361 ctgtccttgc gggggcagcc actcctcgac cagcggtcgc acctccatct cgtcagtcat
     3421 ccacgtcgcc cgggtcgtgg tactcgccgc agagcctgtc ctcggcggcc tcgcgcgcgt
     3481 cctccctggc cttctgcgcg tcctcggccc gcttccgggc gagcttctgc gcgggggtct
     3541 cggtggcctc gcgggcctcc tcggcggcgt cccggacgtc atcggcccgc tcccgcgcgt
     3601 ccctcgccgc gcactgggcc accttgccgg ggttcgagga cggcaccggg aggatcgccg
     3661 ccctctccgc cgtctggttc gtggtcgggg ccgtctggcc cgcgagcgcc accggcccgg
     3721 ccacaggcgc gggcttgtgg gcctgcgcca tgacgacggc cgcggtcgcg acggcgaagc
     3781 tcgccgcgag ggccagggcg agcacggacc tccagacctt gccggaccgc ggctgcctcg
     3841 gggcccgacc gctcggacgc tgtcccggga acgggagctc ctgcgtgggg tcctcgctca
     3901 tgatcctctc cttcccggct ctccctcgag ccgctgcccg gattcttcga acaagccaag
     3961 ggtggctgag cggcaacggg gtggctaggg acctcagggg tggccaccgg cgccccaagg
     4021 ggtggagtca ccccacccta ggtgccgctg ccgcccgaga cctccgggtc gaggccgggc
     4081 aggccggcca ggtaccaggc cgcggtggcc gcgcggtgcg cggcgcggga ccggtactcc
     4141 gccaggcgac cctgccgccg gtacagcagg ctcggccggt agagctcccg gtcgtcgatc
     4201 ccgggcacgt cggcggcgac ctcgcagccg aggtcgggat cgtcgccgtc ccacgcgatg
     4261 gtgccctcgg cgacggcggc gacgaccgcc gacgtgtccg agacggcgat cgccttggag
     4321 cggccggcgc gcgccgggcc cccgacccgg cccgtgttga ccaggaagac ctccatcggg
     4381 tggtccctga gcaactcggc cagccggttg ccgtgcctcg cgtactcgag cgcgagcggg
     4441 tcggacccgc gccggccgcc ggactcctgc ctgccgtcgg cggccgggcc gtggatctca
     4501 cggagcaggt agtggcgggc ggcctggctg gggtcgatgc gcgcgacggc gggggtcacg
     4561 tgctcgtcgc ggttcaggat caccaggatc gacgcgggcg gtacccggtc gggcgggaac
     4621 cggtcgaccg agcgcaggtt gaacaccgcc cgcccgtgcc gcgtggacgt cgggtcgagg
     4681 aagtcgagct cctcgccctg ctgcgagacg ttctccagat acgcgtccgg cccggtcgcg
     4741 gcggcgtgga ccaggggctg caggagcggg tcgaggccgt aggtcttctc gatgcagccg
     4801 tcctcgctcg gcacgatgcg gccgccgggg agcaggccga cgaagtcgtc ctggaccacc
     4861 cgcgacccgt tgtgacaggt gaaggtggtg gtggacttgc ccgtgcccga gcggccgacg
     4921 atcagcatcg tcctcgggcc ccgcgccgtc gggacgacct tgcaggcggc gtgcatgacc
     4981 agaccgccgg catcgtggaa gcgccagctc cacatccgga ggacggcctt cttcgcctcc
     5041 ccgaagtagt cggagccgac gatccgggtc acgccctcct ccggccagac cgcgaccacg
     5101 cggccgtcgg ggtagccggg gacgccgagg ttgggggtgc agatgaccgt cagttccgga
     5161 gcgaccggcg cgtcgccgac caccgggtcg tagtagaggt gccgctgcat gccggcgatg
     5221 ccggcgttgg cccgctcgac gaccaggcgg gccagggtgc ggtgcgggcc ggcatggccg
     5281 atgaagccgt cgacgaccac catgtcgtgc gactcgatgt aggcgtcctg gacggcggcg
     5341 atcagcgcgc cctcggcgcg gcccatcggc tgtccagggt gccggtcggg gcggtcgtcg
     5401 acgacgaagg tggcgctcct caggcggtcc tgcacccgcg tccgcacgtt cacgttgccg
     5461 aacctggtcc ggcgggcggc gggcatcgag tcggtgagct ccgcgagctc gccctctgac
     5521 gggcggaacc tgacggaccc ggcccgcagg ccggcgtcgg cggtcagcgc gggtgtcatg
     5581 gccggtccct ccgcccctca gggctgtcgt cgtccaggct cgtcgagcgg agccagcgtg
     5641 cggcagggca ggcgccgcga ccagagacca gaggggtggt gtcgacctcc ccgcgggggt
     5701 ggaaccgggc accctaggtg gagtctcccc cgggcgcgcc caggcttcca gccctgccgg
     5761 cggtgggata cgatccaggc ccgaaaggag cgcgctcagg tcgatcgagc cgccgcgtac
     5821 cagacgatgg ctgaccattc cgctgcgacc tcgtcctccg agcccaaggc tcggacggat
     5881 gcggagcgcc gcgggacgcc cctcctcgtg taccgggacg gtggcggcga gcagcaggtc
     5941 gtcgtgctcg aggagctggg atccccgttc acggtcggcc gcggggcagc cgccgacatc
     6001 cggctcgact gggacgccga ggtctcccga ctccacgcgg
//
LOCUS       DASPYY010000444         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1339412, whole genome shotgun sequence.
ACCESSION   DASPYY010000444 DASPYY010000000
VERSION     DASPYY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339412"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1375
                     /locus_tag="VEP73_06940"
     CDS             188..1375
                     /locus_tag="VEP73_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26202.1"
                     /translation="MSSDPQRPGLWTRLVAHLGGEVPATTLEAYRRAGGSVYELLSDV
                     EQRRAELMLTGADPWSPDAPGRALELCAWNAFSLQLLGDELLASDYQANPSTVGFVPP
                     VTAAQVHAFYDQVEEWLSRARQALDNPVYRLDAYVPAELPAWSDAERCPREHLEALLV
                     AGRRLRQRAEIALGDLTRSVPAGREPDAARLRQLLAVVDTAIDYADRLHRGDVGEEVH
                     ERIEQSVRLAIETAYLLGQLVAMPALLDRQDRPAPGRMRRKLPLPGQPGFDPWCLTDP
                     RVCSEWQRDPRARRAIERLWRHDPRPDVTLGIQGKINAALARGAVAYATDHHGRPVGQ
                     YFRCPWAPIYVARRPITIAGRRLETMQQFTLDISAEPLDGGGKFRCRMLRGPFEDAEA
                     VDW"
     gene            1447..2877
                     /gene="gndA"
                     /locus_tag="VEP73_06945"
     CDS             1447..2877
                     /gene="gndA"
                     /locus_tag="VEP73_06945"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HYT26203.1"
                     /translation="MAGSAEIGITGLAVMGQNLARNVARHGIPIAVHNRTAARTEELL
                     ARHGDEGPLIGTASVEEFVGALARPRRILVMVKAGPPVDAVVDELAPHLEAGDIVIDG
                     GNSWFEDTRRREQALRDRGLHFVGTGISGGEEGALHGPSIMPGGSAEAYGAIEHVLTT
                     IAAKVDGTPCCTHVGPDGAGHYVKMVHNGIEYADMQLIAESYDLLASALGLGAAELAE
                     VFDRWNQGDLESFLIEITARVLRQVDAATGRPLVDVILDEAEQKGTGRWTVQSALELG
                     VPITGITEAVFARMLSARKDDRVAAAKVLPGPGVGARAADRLVDDVRDALYASKVVAY
                     AQGFEQMAAASEQYGWGIDLGAMATIWRGGCIIRARFLDRISDAFQAEPGLRSLLLAD
                     YFRDAVAGAQQAWRRVVGTAVELGVPVPAFASSLAYYDGYRRERLPANLVQGLRDFFG
                     AHTYRRTDRPGSFHTMWSEDGREVEV"
     gene            2895..3344
                     /locus_tag="VEP73_06950"
                     /pseudo
     CDS             2895..3344
                     /locus_tag="VEP73_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(3849..4433)
                     /locus_tag="VEP73_06955"
                     /pseudo
     CDS             complement(3849..4433)
                     /locus_tag="VEP73_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          579 a         1654 c         1620 g          586 t
ORIGIN      
        1 ggggaaggcg accgccgcgc gcgcgcgccg gccgggtgcg tccacgtcca ggtgaagcat
       61 cgtgtcctcc tctcggtcat cccccgccca ggcgatggag ccgttcgagc accggcgggc
      121 actgtcgcac ctgccgcggc cgtatcctac tgtccggggg gaaggcgatc ggcgaagaag
      181 gagaagaatg tcgtcggatc cccagcgacc cgggctgtgg acgaggctcg tcgctcacct
      241 gggcggggag gtgccggcca ccaccctgga ggcgtaccgg cgggcgggtg ggagcgtcta
      301 cgagctgctg agcgacgtcg agcagcgccg ggcggagctg atgctcacgg gcgcggaccc
      361 gtggtcgccc gatgcgcccg gccgggcgct ggagctgtgc gcctggaatg cgttcagcct
      421 tcagctcctc ggcgacgagc tgctcgcctc cgactaccag gccaacccgt cgacggtcgg
      481 gttcgtgccg ccggtgaccg ccgcgcaggt gcacgccttc tacgaccagg tcgaggagtg
      541 gctgagccgt gcccgccagg ccctggacaa cccggtctac cggctggacg cgtacgtccc
      601 cgcggagctt cccgcctgga gcgacgccga gcgctgcccg cgcgagcatc tcgaggcgct
      661 gctcgtcgcc ggccgcaggc tgcgccagcg cgccgagatc gcgctcggcg acctcaccag
      721 gagcgtgccg gccgggcgcg agccggacgc agcccggttg cgccagttgc tcgcggtcgt
      781 ggacaccgcg atcgactacg ccgaccggct ccaccgtggt gacgtcggcg aggaggtgca
      841 cgagcgcatc gagcagagcg tccggctcgc catcgagacc gcgtatctgc tcggccagct
      901 cgtggcgatg ccagcgctgc tcgaccggca ggaccggcca gcgcccggtc ggatgcggcg
      961 caagctgccg ctgcccggcc agcccggctt cgacccgtgg tgcctgaccg atccgcgagt
     1021 gtgctccgag tggcagcgtg acccgcgtgc ccgcagggcg atcgagcggc tctggcgcca
     1081 tgacccgcgg ccagacgtca cgctggggat ccaggggaag atcaacgccg ccctggcgcg
     1141 cggggcggtc gcctacgcga ccgaccatca cgggcgcccc gtcggccagt acttccgctg
     1201 cccgtgggcg ccgatctacg tggcgcggcg tcccatcacg atcgcggggc ggcgcctcga
     1261 gacgatgcag cagttcaccc tcgacatctc ggccgagccg ctcgatggtg gcggcaagtt
     1321 ccggtgcaga atgctgcgcg gcccgttcga ggacgccgag gccgtcgact ggtgaagggc
     1381 taagatcgac ctcggccagg caacggcgga gccacggctc cggctcgaca gaacaggagg
     1441 caccctgtgg ctggcagcgc tgagatcggc atcaccggcc tcgcggtcat gggccagaac
     1501 ctggcccgca acgtggcccg ccacgggatc ccgatcgcgg tccacaaccg taccgccgcc
     1561 cgcaccgaag agctgctcgc ccgccacggc gacgaggggc cgctcatcgg gacggccagc
     1621 gtcgaggagt tcgtcggcgc gctcgcccgg ccgcggcgga tcctcgtgat ggtgaaggcg
     1681 ggcccgcccg tcgacgcggt cgtcgacgag ctcgcgcccc atctcgaggc gggtgacatc
     1741 gtcatcgacg gcggcaactc ctggttcgag gacacccgcc ggcgcgagca ggcgctgcgc
     1801 gaccgggggc tccacttcgt cggcaccggc atctccgggg gcgaggaggg cgccctgcac
     1861 gggccgagca tcatgcccgg cggctccgcc gaggcctatg gggcgatcga gcacgtactg
     1921 accacgatcg cggccaaggt cgacggcacc ccgtgctgca cccacgtcgg cccggacggg
     1981 gcggggcact acgtgaagat ggtgcacaac ggcatcgagt acgccgacat gcagctcatc
     2041 gccgagtcct acgacctgct cgcctcggcg ctcgggctcg gcgccgccga gctggccgag
     2101 gtcttcgacc gctggaacca gggggacctc gagtcgttcc tcatcgagat caccgcccgc
     2161 gtgctgcgcc aggtggacgc ggccaccggc cggccgctcg tcgacgtgat cctcgacgag
     2221 gccgagcaga agggcaccgg gcggtggacg gtgcagtcgg ccctcgagct cggcgtgccg
     2281 atcaccggca tcaccgaggc ggtgttcgcc cgcatgctct cggcccgcaa ggacgaccgg
     2341 gtcgccgcgg ccaaggtgct gcccggccct ggcgtcggcg cgagggccgc cgaccgcctc
     2401 gtcgacgacg tgcgcgacgc cctctacgcc agcaaggtgg ttgcctacgc ccagggcttc
     2461 gagcagatgg cggccgcgag cgagcagtac ggctggggga tcgacctcgg cgccatggcg
     2521 accatctggc ggggcggctg catcatcagg gcacgcttcc tcgaccgcat ctccgacgcc
     2581 ttccaggccg agccggggct gcgcagcctc ctgctcgccg actacttccg cgacgccgtg
     2641 gccggtgcgc agcaggcgtg gcggcgggtc gtcggcaccg cggtcgagct cggcgtgccc
     2701 gtccccgcct tcgcctcctc gctggcctac tacgacggct accgccgcga gcgcctgccc
     2761 gccaacctcg tccaggggct gcgggacttc ttcggcgcac acacctaccg gcgcaccgac
     2821 cggccaggct cgttccacac gatgtggtcc gaggacggcc gcgaggtcga ggtatagagg
     2881 tcgaggtgga ggagatggcc gtcaccgagg gcgccgcccc acccgccgcc ggcaccttca
     2941 cgtccgtcgg ccccgcctcc ggcgaggtcg tcggcagctt cccggtcgac gggcccgagg
     3001 cggtcgcagc caccgtcgcc cgggcgcgcc aggccgctgg ctggtgggcc gggctcggct
     3061 tcgaggggcg gcggaagcgg ctgtcggcct acaagggcca gctcgcccgc cgctccgacg
     3121 agctctgcga cctcgtccac cgcgagaacg gcaagcccct cgacgacgcc ttcatcgagg
     3181 tgctcgtcac cgtcgagcac ctggactggg ccgcccgcaa cgccgcgaag gcgctcgagc
     3241 cccggcgggt ccgcagcacc ctgctgctcg ccaaccaggg cgcgtcggtc gagtacctgc
     3301 ccctcggcgt cgtcggcgtc gtcggcccgt ggaacgaccc gctgcactga ggtacccgat
     3361 gcacccaggt ccccgatggg cagcacgcct gtgacgcgca cgtccggatg gtgcctccag
     3421 cgcccatgcc gggcgccctc ggcgacgacc tgcacgcgga tgtccagcgc ggcgaacacg
     3481 tcccgcatcc gctccggcgt cgggtcgtcc agccgctccc gggccgtctc ggcgagcttc
     3541 cccacgtcgc gcagcagcgc cgcgcgctcg gcgttcgcct gcgcccacgc gcgggcccgg
     3601 gcccgtagct cctcgagcgc gtcgatgtcg cggttgacct cggccagggc gtcggcgacg
     3661 gcctcggggc cggtgtcggc ggcggccagc gcgaggttgg tccggcggcg cttggcggcc
     3721 gtaaggcgcc ggtcgaggtc ggagagctgg ccggcctcgg cgcgcagctc gccgccgcgc
     3781 tcctcgaggt actgctgcgc cagcccgatg agccgctcgg ggtcggcgag cacctcgcga
     3841 acctcgcccc acacggtctc ctcgagccag tcggcgtcga tccactggca gtagcagcgc
     3901 tggtcggtcc cgttgaactt cgggtcgttg tagcggcact cgtactgccg ctggtcccgg
     3961 tggccgaagt cgttgcggta gaccccgacg tacggcccgc cgcagaggcc gaccacgcgg
     4021 ccggtcagcg ggtagacccg gttgcgcggc cggacgacgg atctcgcgtc cagcgccttg
     4081 tggacctcgg ccatgcggtc ggcgtcgagg atcgcggggc cgcgcacggg gatctcgcgg
     4141 ccggccttgc gcagcaccca cgccccggcg aagtggtcgg cgcggagcat catgcgcagg
     4201 ctgccgttgt tccagcgggc gctgtgccgc gggggccggc cgagcgcgtt gagcctgcgg
     4261 gcggcctgca cgcacgacag gctctcgtcg gcgaccagct cggcggcggc gcggatcgtc
     4321 gccgcctcgg cctcctcgag cgcgacgacc ttgcgcttgg ggttgtcggg cgccgggacg
     4381 atccgccagc cgaacggcgc ggacccgacc ggccagtagc ccgcctcggc cacggccag
//
LOCUS       DASPYY010000445         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1902710, whole genome shotgun sequence.
ACCESSION   DASPYY010000445 DASPYY010000000
VERSION     DASPYY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902710"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VEP73_06960"
     CDS             <1..766
                     /locus_tag="VEP73_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYT26204.1"
                     /translation="DAGAVWITLNRPAALNALDTPTKEALVAALEQAAADPSVRAVAL
                     TGAGRAFCVGEDLRGLEAGYAEGRAPELGDTLQRHYAPAVRLLAEMPKPTVAVVNGVA
                     AGAGASLAFACDLRVASAAASFRLAFGGVGLVPDAGATYHLPRLVGLAKAMELAMLGG
                     TVRADEARSIGLVSRVFPAEELAVEAAALVAALAAGPTLALARTKALLRDGLHADLAW
                     ALDAEAGAQVACGQTSDHLEGVAAFLEKRAPRFQGA"
     gene            793..>1521
                     /locus_tag="VEP73_06965"
     CDS             793..>1521
                     /locus_tag="VEP73_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26205.1"
                     /translation="MADAMASDFQLFIDGRLGEAASGATFETVDPSTGRPHATVARAG
                     AEDAGRAVAAARAAFEDGRWSGLRPEKRAAVLNEVAARLRQHAERLVELEVRDSGGTI
                     RKTKASDVSGAAFTFRTFADFATRVPTEQPLPFTVAPGPSHNYLRREPVGVCAGIVPW
                     NFPIQNAAWKIAPALAAGNTIVLKPAEQTPCTAVELAKLCAEAGVPDGVVNVLPGFGP
                     EAGEPLVTSPGVDKVSFTGSTEVGR"
BASE COUNT          184 a          562 c          600 g          175 t
ORIGIN      
        1 tgacgcgggc gccgtctgga tcacgctgaa ccggcccgcc gcgctgaacg ccctcgacac
       61 cccgacgaag gaggcgctcg tcgcggcgct ggagcaggcg gccgcggacc cgtcggtgcg
      121 cgcggtggcc ctgaccgggg cgggccgcgc gttctgcgtc ggcgaggacc tgcgcgggct
      181 cgaggccggg tacgcggagg gccgggcgcc cgagctgggc gacacgctgc agcgccacta
      241 cgccccagcc gtccgcctgc tcgcggagat gccgaagccg accgtcgcgg tggtgaacgg
      301 ggtcgcggcc ggcgcgggcg cgtcgctcgc gttcgcatgc gacctgcggg tggccagcgc
      361 ggcggcgagc ttcaggctcg cgttcggcgg ggtcgggctg gtccccgacg ccggcgcgac
      421 ctaccacctg ccgcggctcg tcggcctggc caaggcgatg gagctggcca tgctcggcgg
      481 caccgtcagg gcggacgagg cgcgctcgat cgggctggtg agcagggtgt tcccggccga
      541 ggagctcgcc gtggaggccg ccgcgctggt ggcggcgctt gccgccgggc cgaccctggc
      601 cctggcccgc accaaggcgc tgctgcggga cggcctgcac gcggacctgg cctgggcgct
      661 cgacgcggag gcgggcgccc aggtggcctg cggccagacc tccgaccacc tggagggggt
      721 ggccgcgttc ctcgagaagc gcgcgccccg cttccagggc gcgtagcgcg acccacggca
      781 aggagcgaca cgatggcgga cgcgatggca tcggacttcc agctcttcat cgacggccgg
      841 ctcggcgagg cggccagcgg ggcgaccttc gagacggtcg acccgtcgac cggccgcccg
      901 cacgccaccg tcgcccgcgc cggcgccgag gacgccgggc gggcggtcgc cgccgcccgg
      961 gccgcgttcg aggacggccg ctggtcgggc ctgcgccccg agaagcgcgc cgcagtgctg
     1021 aacgaggtcg cggcgaggct gcggcagcac gccgagcggc tcgtcgagct cgaggtgcgg
     1081 gactcggggg gcacgatccg caagacgaag gcgtccgacg tgagcggggc cgcgttcacc
     1141 ttccggacct tcgcggactt cgccacgcgc gtccccaccg agcagccgct gccgttcacg
     1201 gtcgcgccgg gaccgagtca caactacctg cggcgagagc cggtcggggt gtgcgccggg
     1261 atcgtaccgt ggaacttccc catccagaac gcggcctgga agatcgcgcc ggcgctggcc
     1321 gccggcaaca ccatcgtgct gaagccggcc gagcagacgc cgtgcaccgc ggtcgagctg
     1381 gccaagctgt gcgccgaggc cggcgtgccc gacggcgtcg tgaacgtcct gcccggcttc
     1441 ggccccgagg ccggcgagcc gctcgtcacc tcgcccggcg tggacaaggt ctcgttcacc
     1501 ggctcgaccg aggtgggccg c
//
LOCUS       DASPYY010000446         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2042458, whole genome shotgun sequence.
ACCESSION   DASPYY010000446 DASPYY010000000
VERSION     DASPYY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042458"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VEP73_06970"
     CDS             <1..675
                     /locus_tag="VEP73_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26206.1"
                     /translation="MLREIQAGPPPHWEHAAAWAERMRGWAAACDRLVVQSADAAEQA
                     RVLLGVDPARVTCVPNGVDVGRFDRQPARGGARVGFWRRWLVEDPQGWDHSGVPGSVA
                     YGERDLEPFRSDGPVLLYVGRFTAVKRVGLLVRAYALARQRFRARAPLVLLGGHPGEL
                     EGRHPLDEIAGAGVPDVFLGGRARAGHDRGAGSHRLARPSRRRGGTRRRAGGGGQRPG
                     AARGDG"
     gene            566..874
                     /locus_tag="VEP73_06975"
     CDS             566..874
                     /locus_tag="VEP73_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26207.1"
                     /translation="MEPGRTGWLVPPDDEEALAAALVEAVNDPERRAAMGDAAWTASR
                     ARYSLEAVVAGIAGVYGHALLAQPCMRPAPRRLARPGRPEPGGSSHAVAPSCCRLPPR
                     "
     gene            900..1694
                     /locus_tag="VEP73_06980"
     CDS             900..1694
                     /locus_tag="VEP73_06980"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYT26208.1"
                     /translation="MRRVAVANQKGGVGKTTTVVNLGAALAELGRRVLVVDLDPQANA
                     STWLGTVDARALYEVFENGAPLENAVRPTEVKGLDLVPSSSWMTRAERNAATQAGAEM
                     ILRQAMEELPPDTWDVVLCDCPPALGLLSYSALAACDEVLIPVQTRVMPLSGLVALSQ
                     TIDTVRVRLNRRLRLSAVLPCQVDRTTLSREIVDALRQRFGDVVMETMIRDSVRVAES
                     PGHRLPVIVYAPDSAVATDYRAAAVEFDARSAAHGQAQADGRVQRA"
BASE COUNT          213 a          643 c          679 g          241 t
ORIGIN      
        1 atgctcaggg agatccaggc cgggccgccg ccccactggg agcacgcggc cgcctgggcg
       61 gagcggatgc gcggctgggc ggcggcgtgc gaccggctgg tcgtccagtc cgcggacgcg
      121 gccgagcagg cgcgggtgct gctcggcgtc gacccggcca gggtgacctg cgttccgaac
      181 ggggtcgacg tcgggcgctt cgaccggcag ccggcgcgcg gcggggcgcg ggtggggttc
      241 tggcgccgct ggctcgtcga ggacccgcag ggctgggatc actcgggcgt gcctggcagc
      301 gtcgcctacg gcgagcggga cctcgagccg ttccgctcgg acgggccggt gctgctctac
      361 gtgggccggt tcacggccgt aaagcgggtc gggctcctcg ttcgcgccta tgcgctggcg
      421 aggcagcggt tccgtgcgcg ggcgccgctc gtgctcctcg gcggtcatcc gggagagctt
      481 gagggccgcc acccgctcga cgagatcgcg ggcgccggcg tgcccgacgt cttcctcggt
      541 ggacgcgcac gggccggcca cgatcgtgga gccgggtcgc accggctggc tcgtccctcc
      601 cgacgacgag gaggcactcg ccgccgcgct ggtggaggcg gtcaacgacc cggagcggcg
      661 cgcggcgatg ggtgacgccg cgtggacggc gagccgcgcg cgctactccc tggaggcggt
      721 cgtcgccggg atcgccggcg tctacggcca cgcgctgctc gcacagccgt gcatgcgccc
      781 cgctccccgg cgcctggccc ggccgggaag gccggaacct ggcggctcgt cccacgcggt
      841 ggcgccgtcg tgttgtaggc tgccccctcg gtaggaagcc tctcgacagg tggtgccccg
      901 tgcgacgtgt cgccgtcgcg aaccagaagg gcggggtcgg gaagacgacc acggtggtga
      961 acctcggcgc cgcgctggcg gagctcggcc gccgggtgct cgtggtcgac ctcgacccgc
     1021 aggccaatgc gagcacctgg ctcggcaccg tcgatgcccg ggccctctac gaagtgttcg
     1081 agaacggcgc gccgctggag aacgccgtcc ggccgaccga ggtcaagggc ctcgacctcg
     1141 tgccgtcctc ctcgtggatg acgagggccg agcggaacgc cgcgacccag gctggcgcgg
     1201 agatgatcct ccgccaggca atggaggagc tcccccccga cacctgggac gtcgtcctgt
     1261 gcgactgccc gccggccctc ggcctgctct cgtactcggc gctcgccgca tgtgacgagg
     1321 tgctgatccc cgtgcagacg cgggtgatgc cgctctccgg cctcgtcgcg ctcagccaga
     1381 ccatcgacac cgtccgggtc cggctgaacc ggcgcctgcg gctctccgcg gtgctgccgt
     1441 gccaggtcga ccgcaccacc ctgtccaggg agatcgtcga cgcgctccgc cagcggttcg
     1501 gcgacgtcgt catggagacg atgatccggg acagcgtgcg ggtggccgag tcgcccgggc
     1561 accggctgcc ggtcatcgtc tacgcacccg acagcgccgt ggcgaccgac taccgggcgg
     1621 ccgcggtcga gttcgacgca aggagcgctg cgcatggtca ggcgcaagcg gatgggcgcg
     1681 tacaacgcgc ttgacgacct gctccccgga ccgccgcagg cgcggccggg gctcgagcgc
     1741 tctccccacc cggtgtccgg accgtccaag cggatg
//
LOCUS       DASPYY010000447         5506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1200960, whole genome shotgun sequence.
ACCESSION   DASPYY010000447 DASPYY010000000
VERSION     DASPYY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200960"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="mazG"
                     /locus_tag="VEP73_06985"
     CDS             <1..875
                     /gene="mazG"
                     /locus_tag="VEP73_06985"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HYT26209.1"
                     /translation="QHVEIEAVYPLGEPKGAALLDLVETETRLRGPGGCPWDREQTHA
                     SLARHLVEESYEVLDAIDEGDPEHLREELGDLLLQIVFHAQIAEDAGQFDVDGVARSI
                     TEKLVRRHPHVFAGVRVESAGEVVANWESIKREQEGRTDPLAGIPSALPALQLAAKLQ
                     RRAAADGFAWPDRDGPAAKVAEELREVLAASERADLEWEVGDLLFAAVALARSVEVDP
                     EAALRRTARRFRARYAAARAAVEADGLDPASLDVERWLAYWDAAKRSLAAAPEGARAE
                     AEETAEEIEAGPEA"
     gene            complement(881..1600)
                     /locus_tag="VEP73_06990"
     CDS             complement(881..1600)
                     /locus_tag="VEP73_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26210.1"
                     /translation="MSRREPVPPSRRPLGRWLGLVAVTAVLAAGCSFASGMLRTTTAL
                     REAGIDNPDIAGGNGRVTVTYDAHASGPDLPAEQDRVAGVIWRNLPFRFSELAVTPRG
                     ASPPPSAGTRTYTREELEARLGPRPSGLDNTARDIEASARTTVRNVIIGLIVGGIVLL
                     ALVTLIIVLVVRASRRARPPVPAPAVGGWGPAPPGGQGWGQPQAPGWGQPPPPGPGRQ
                     QAPGWGQQRPEPPEQGPPPER"
     gene            1801..3099
                     /gene="eno"
                     /locus_tag="VEP73_06995"
     CDS             1801..3099
                     /gene="eno"
                     /locus_tag="VEP73_06995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYT26211.1"
                     /translation="MAVIELIVAREVLDSRGNPTLECEVQLEDGTTGRAIVPSGASTG
                     RFEAVELRDGGPRYGGKGVWNAVRNVNETIAPKLLEEEDALDQRAIDRLLIDLDGTGN
                     KRKLGANAILGVSMAVARAAADYLDVPLFRHLGGFSAHVLPVPLMNVLNGGVHADNDV
                     DFQEFMVVPVGAPSFAEGLRMGAEVYQALKGVLKDAGLGTGVGDEGGFAPNLPGNEAA
                     LELLVQAIGKAGFDPGDEVALAMDPAASEFFRDGRYELAGEGRSLDSDGMIELWRTLC
                     DRYPIVSIEDGLAEDDWGGWVALTDALGDRVQLVGDDLFVTDVERIRRGLDEGAANSV
                     LIKVNQIGTLTETFDAVELAHRGGYTTVVSHRSGETEDTTIADLAVAAGSGQIKSGAP
                     CRSERVAKYNQLLRIEELLGDTARYRGRLAFPRTYRGDDR"
     gene            3096..3560
                     /locus_tag="VEP73_07000"
     CDS             3096..3560
                     /locus_tag="VEP73_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HYT26212.1"
                     /translation="MRRLRPAALPARLPAGGPVSPAGSLPGTGRPPLGLTPRGVLLFL
                     ALFALAATAVYPLREYVAQHNRILALEAKHQALQAENERLAREQARLNDPAYVEQLAK
                     RDFQMVHPGEEAWQLTGTPPADARAPARPAPAPRRDRPWYQRLWEHLTGSAK"
     gene            3691..4062
                     /locus_tag="VEP73_07005"
     CDS             3691..4062
                     /locus_tag="VEP73_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYT26213.1"
                     /translation="MPAEIGAIVPGTVVRLAPYGAFIKLETGETGLVHISEIDRNYVR
                     SVEEHLRIDDAVVVKVVGMKDDGKIDLSIKQAAPDWQPEATRPRRAAKDPEFEQKLKR
                     FMRSSEERLVDVKRQREGRRG"
     gene            4076..4738
                     /locus_tag="VEP73_07010"
     CDS             4076..4738
                     /locus_tag="VEP73_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HYT26214.1"
                     /translation="MPRTPNRSPRRPDPAALPDPAHVPWAPFASDGLADGRAEPSWAA
                     SLPEASASERQLVEAQIGRRARGSLAVAARCRYGLPTVVRTAPRLPDGTPFPTLYWLT
                     CPAARVAVGRLEAAGWNARLSERVAAEPALAGAHADAHASYLAQRDALERLPGDPGVG
                     GLPGRVKCLHVLYAHEVATGFDPVGRVVRRAVDPVDCPGPCVDAAPGGRAGRPEHRRR
                     SG"
     gene            4747..>5506
                     /locus_tag="VEP73_07015"
     CDS             4747..>5506
                     /locus_tag="VEP73_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HYT26215.1"
                     /translation="MRLAALDVGTNSTRLLVADVEGGRIVAEHAREMVITRLGRGVDR
                     ARRFDPGALARTLEVIGGYAATCDRLGVDAVRVVATSATRDAGNAGELASGVRRLLGV
                     EPEVLTGDQEAAATFAGATAELPGEGRTLVVDIGGGSTELIVGGRPARAAAPVPVPAA
                     PGADGPVPSRLVPDAMISLDIGCVRLTERHLHTDPPTAAETAALRADARAQLHQVAAV
                     LDPASADRVVGVAGTVTTVTAIALGLDRYDPARIH"
BASE COUNT          702 a         2134 c         2012 g          658 t
ORIGIN      
        1 gccagcacgt cgagatcgag gctgtctacc cgctcggcga gcccaagggc gccgccctgc
       61 tggacctcgt cgagaccgag acgcgcctcc gcggcccggg cggctgcccc tgggaccgcg
      121 agcagaccca cgcctcgctg gccaggcacc tggtcgagga gtcgtacgag gtgctggacg
      181 ccatcgacga gggggacccc gagcacctcc gcgaggagct cggcgacctg ctcctgcaga
      241 tcgtgttcca cgcgcagata gccgaggacg ccggccagtt cgacgtcgac ggggtcgccc
      301 ggtcgatcac cgagaagctc gtccgccgcc acccccacgt cttcgccggc gtccgggtcg
      361 agtcggccgg cgaggtggtc gccaactggg agtcgatcaa gcgggagcag gagggccgga
      421 ccgacccgct cgccggcatc ccgtcggccc tgccggccct gcagctcgcg gccaagctgc
      481 agcggcgcgc cgccgcggac gggttcgcct ggcccgaccg ggacgggccg gccgcgaagg
      541 tcgccgagga gctccgggag gtgctcgcgg cgtcggagcg ggccgacctc gagtgggagg
      601 tgggcgacct gctgttcgcc gccgtggccc tggcccgctc ggtggaggtg gacccggagg
      661 ccgcgctgcg ccgcaccgcc cgccggttcc gggcgcgcta cgccgccgcc agggcggcgg
      721 tcgaggccga cggcctcgac ccggcctccc tggacgtcga gcggtggctc gcgtactggg
      781 acgcggccaa gcgctcgctc gccgccgccc ccgagggcgc gcgggccgag gcggaagaga
      841 ccgccgagga gatcgaggcc ggcccagagg cgtgaccggg tcaccgctcc ggcggcggcc
      901 cctgctccgg cggctccggc cgctgctgcc cccagcccgg cgcctgctgc cgccccgggc
      961 ccggcggtgg cggctgcccc cagcccggcg cctgcggctg gccccagccc tggccgcctg
     1021 gcggggccgg gccccagccg ccgacggccg gggccgggac cggcggccgg gcccggcggc
     1081 tggcccggac gacgagcacg atgatgaggg tgacgagcgc aagcaggacg atcccaccga
     1141 cgatcaggcc gatgatgacg ttcctgaccg tggtgcgggc gctcgcctcg atgtcccttg
     1201 ccgtgttgtc gaggccggag ggtcgcgggc cgagacgggc ctcgagctcc tcgcgggtgt
     1261 aggtgcgcgt gccggccgac ggcggcggcg aggcgccacg gggggtgacg gccagctcgg
     1321 agaacctgaa cgggaggtta cgccagatga ccccggccac cctgtcctgc tcggcgggca
     1381 gatcgggccc gctcgcgtgc gcgtcgtagg tcacggtcac ccggccgttg ccgccggcga
     1441 tgtccgggtt gtcgatcccg gcctcccgca gggcggtcgt cgtcctcagc atgccgctcg
     1501 cgaagctgca gccggccgcg agcaccgccg tcacggccac gagaccgagc caccgtccca
     1561 acggccggcg gctcggcggc accggctccc ttctcgacat cgcgcggctc cttcgcccac
     1621 cgggtgtctc ctccgcgcgc gaggataccc tgcgggccgt ggcggaacgg gggcgccggc
     1681 tcgcgtcggc tgtggacgcg tcggctgtgg aaaggtcccc ggccacggcg agcggcgatg
     1741 gtatgctcgc gcgcgcccgg accgccgcga acgcagggag atcttcccca catggcttcc
     1801 atggccgtca tcgagctcat cgtcgctcgg gaggtcctcg actcccgcgg caaccccacc
     1861 ctggaatgcg aggtccagct cgaggacggg accacggggc gggccatcgt cccgtcgggg
     1921 gccagcaccg gccggttcga ggccgtcgag ctgcgcgacg gcggcccccg ctacggcggc
     1981 aagggcgtct ggaacgccgt ccgcaacgtc aacgagacga tcgccccgaa gctcctcgaa
     2041 gaggaggacg cgctcgacca gcgcgcgatc gaccggctgc tgatcgacct cgacggcacc
     2101 gggaacaagc gcaagctggg ggccaacgcg atcctcgggg tctcgatggc ggtggccagg
     2161 gccgccgccg actacctcga cgtgccgctg ttccgccacc tcgggggatt ctcggcccac
     2221 gtgctccccg tcccgctgat gaacgtgctg aacggcggcg tccacgcgga caacgacgtc
     2281 gacttccagg agttcatggt cgtgcccgtc ggcgcgccgt cgttcgccga ggggctccgc
     2341 atgggcgccg aggtgtacca ggccctcaag ggcgtgctga aggacgccgg gctcggtacc
     2401 ggggtcggcg acgagggcgg cttcgcgccg aacctgccag gcaacgaggc agcgctcgag
     2461 ctgctggtcc aggccatcgg caaggcaggg ttcgaccccg gcgacgaggt cgcgctcgcc
     2521 atggacccgg ccgccagcga gttcttccgg gacggccgct acgagctcgc cggggagggc
     2581 cgctcgcttg acagcgacgg catgatcgag ctgtggcgga cgctctgcga ccgctacccg
     2641 atcgtgtcca tcgaggacgg cctggccgag gacgactggg gcggctgggt ggcgctcacc
     2701 gacgcgctcg gcgaccgggt ccagctcgtc ggcgacgacc tgttcgtcac cgacgtcgag
     2761 cgcatccggc gcggcctgga cgagggggcg gccaactcgg tgctcatcaa ggtgaaccag
     2821 atcggcaccc tgaccgagac cttcgacgcc gtcgagctcg cccatcgcgg cggctacacc
     2881 acggtcgtga gccatcgttc gggcgagacc gaggacacca cgatcgccga cctcgccgtg
     2941 gccgccggct cggggcagat caagagcggg gcgccctgcc gcagcgagcg cgtcgccaag
     3001 tacaaccagc tcctccgcat cgaggagctg ctgggggaca ccgcccgcta ccggggccgg
     3061 ctcgcgttcc cgcgcaccta ccggggcgac gaccgatgag acgcctccgg ccggccgccc
     3121 tgcccgcccg cctcccggcg ggcgggccgg tcagcccggc cggctcgctc ccgggcaccg
     3181 gccgacctcc cctcggcctc acgccgcgcg gggtgctcct cttcctcgcc ctcttcgcgc
     3241 tcgccgccac cgccgtctac cccctgcgcg agtacgtggc ccagcacaac cgcatcctgg
     3301 cgctcgaggc caagcaccag gcgctccagg ccgagaacga gcgcctggcc cgcgagcagg
     3361 cccggctcaa cgatcccgcc tacgtcgagc agctcgccaa gcgggacttc cagatggtgc
     3421 atcccggcga ggaggcctgg cagctcaccg gcaccccgcc cgcggacgca cgggcgccag
     3481 cgcggccggc acccgcgccg cggcgcgacc gtccctggta tcagcgactt tgggagcatc
     3541 tgaccgggtc ggcgaagtag tccgccaagg cggccgaagt ttcacatatg cgtttgttga
     3601 cggcccgaaa ggccgtccct acgatggtgc cgagccagca cccctcggct ccacggagcc
     3661 cccacagcag aaggagcgtc ggtcgccagc atgccagccg agatcggggc catcgtcccg
     3721 ggcacggtcg ttcgcctcgc cccctatgga gcattcatca agctcgagac gggcgagacg
     3781 ggccttgtcc acatctccga gatcgaccgg aactacgtcc gttccgtcga ggagcacctt
     3841 cggatcgacg acgcggtcgt cgtcaaggtc gtcgggatga aggacgacgg caagatcgac
     3901 ctgtcgatca agcaggccgc acccgactgg cagccagagg cgaccaggcc gcgccgcgcg
     3961 gccaaggacc ccgagttcga gcagaagctc aagcggttca tgcgctcgag cgaggagcgg
     4021 ctggtcgacg tcaagcggca gcgcgagggc cggcgcggct agcgctggcc ggccgttgcc
     4081 ccgcacgccg aaccgatcgc cccgccggcc cgacccggcg gcgctgcccg accccgcgca
     4141 cgtcccgtgg gcgcccttcg cgtccgacgg cctcgccgac gggcgcgccg agccctcctg
     4201 ggcggcgtcg ctgcccgagg cgtcggcgtc cgagcgccag ctcgtcgagg cccagatcgg
     4261 ccgccgggcg cgtggctcgc tcgcggtcgc ggcgcgctgc cgctacgggc tgcccacggt
     4321 cgtgcgcacc gccccgcggc tgcccgacgg cacgccgttt cccaccctgt actggctgac
     4381 ctgcccggcc gcccgggtcg ccgtcggccg gctggaggcg gccggctgga acgcccggct
     4441 ttccgagcgg gtcgccgccg agccggcgct ggccggggcg cacgcggacg cccacgcgag
     4501 ctacctcgcc cagcgcgacg ccctcgagcg gctccccggc gatcccggcg tcggcggcct
     4561 tcccggccgg gtcaagtgcc tgcacgtcct ctacgcccac gaggtggcca ccgggttcga
     4621 cccggtcggc cgtgtcgtgc ggcgggccgt cgacccggtc gactgccctg gaccgtgcgt
     4681 ggacgccgcc cccggcgggc gcgcggggcg ccccgagcat cgccgacggt cgggctgagg
     4741 ctggccgtgc gcctcgccgc gctggacgtc gggaccaact cgacgcgcct cctcgtcgcc
     4801 gacgtggagg gcggccgcat cgtcgccgag cacgccaggg agatggtgat cacccgcctc
     4861 gggcgcgggg tggaccgcgc ccgccggttc gacccgggcg ccctggcgag gacgctcgag
     4921 gtcatcggcg ggtacgccgc cacctgcgac cggctcggcg tcgacgcggt ccgggtggtc
     4981 gcgaccagcg ccacccgcga cgccggcaac gccggcgagc tcgcgtccgg cgtgcgccgg
     5041 ctcctcggcg tcgagccaga ggtgctcacc ggcgaccagg aggcggccgc cacgttcgcc
     5101 ggggccaccg ccgagctgcc cggcgaggga cgcaccctgg tcgtggacat cggcggcggc
     5161 tcgaccgagc tcatcgtcgg cggccgtccg gcccgtgcgg ccgcgcccgt gcccgtgccc
     5221 gccgcgccgg gggccgacgg cccggtcccg tcgcgcctgg tcccggacgc gatgatctcc
     5281 ctcgacatcg gctgcgtccg gctcaccgag cgccacctgc acacagaccc cccgaccgcc
     5341 gccgagaccg ccgccctgcg cgccgacgcg cgcgcgcagc tgcaccaggt ggccgccgtg
     5401 ctcgacccgg cctcggccga ccgggtcgtc ggggtcgccg gcacggtcac cacggtcacc
     5461 gccatcgcgc tcggcctcga ccgctacgac ccggcgcgga tccacc
//
LOCUS       DASPYY010000448         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_215906, whole genome shotgun sequence.
ACCESSION   DASPYY010000448 DASPYY010000000
VERSION     DASPYY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215906"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VEP73_07020"
     CDS             <1..968
                     /locus_tag="VEP73_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HYT26216.1"
                     /translation="SAAALLHGYATFDPDLEITGVVLNRVASDRHEAMLREALAPSGV
                     PVVGVLRRHADLAAPERHLGLVPAAERATAARRTVAALGEALAAACDLGAIAAIAAGA
                     KSFAASPWSPEPEPGLAPLREGRVRFAVAAGPAFTFVYQENLELLQAAGAELAFFDPT
                     ADEALPAGATGLYLGGGFPETFAEPLAANQPLRAAVRAFAASGRPLLAECGGLLYLAR
                     TLDGQPMAGVVPADARMSDRLTLGYRQAVAAAPSVLTAAGEALRGHEFHYSRLDPPAG
                     TPAAWLLGDERQPEGFTAAGVHASYLHTHWAATPALATRLVSAAA"
     gene            965..1696
                     /gene="cobI"
                     /locus_tag="VEP73_07025"
     CDS             965..1696
                     /gene="cobI"
                     /locus_tag="VEP73_07025"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HYT26217.1"
                     /translation="MTPTPGTAAGRLIGVGVGPGDPELLTVKAVRLLREADAVFVPVA
                     ASGEPGRAETVVRAYVDPSRIQSVEFALGDDPAARQTNWERAGEAVAAVLRAGQVAAF
                     ATIGDPTVYSTFAYLAATVRRLLPGVRVELVSGITAMQDLAARSGLSLVEGSERLALL
                     PFGANAADLAAFDTVVCYKGGRHLPDLLEVLRRAGRLDRALYGARLGLAGEVVRPAAE
                     LPARPGPYLSTLIALPERRGRGSAL"
     gene            1693..>2229
                     /locus_tag="VEP73_07030"
     CDS             1693..>2229
                     /locus_tag="VEP73_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018817503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HYT26218.1"
                     /translation="MSAKVWFVGAGPGAADLLTFRGAAAIAAADVVVWAGSLVAAEVL
                     VHARAGAEVLDSSDRTLEEVTAVYDRAVAEGLVVARVHSGDPTLYGAIQEQIAECERR
                     GLAWEIVPGVSSMAAAAAAVGRELTIPGVAQSVVITRMATRTSSSMPPGERVRELAGH
                     GATMALFLSCSRPGALQRE"
BASE COUNT          231 a          872 c          831 g          295 t
ORIGIN      
        1 gctcggccgc cgcgctgctg cacggctacg ccaccttcga cccggacctc gagatcaccg
       61 gggtggtgct gaaccgggtc gcctccgacc ggcacgaagc gatgctccgc gaggcgctgg
      121 cgccgtcggg cgtgcccgtc gtcggggtgc tgcgacgcca cgcggacctc gccgcccccg
      181 aacgccacct cggtctggtc ccggcggccg aacgcgcgac cgccgcgcgc cggaccgtcg
      241 cggcgcttgg cgaggcgctg gccgccgcct gcgatctcgg cgccatcgcg gccatcgctg
      301 cgggcgccaa gtcgttcgcc gcctcgcctt ggagtcccga gccggagccc ggcctggcgc
      361 ccctgcggga gggccgggtc cgcttcgcgg tcgccgccgg cccggccttc accttcgtct
      421 accaggagaa cctggagctg ctgcaggctg ccggcgccga gctggctttc ttcgacccga
      481 ccgcagacga ggccctgccg gctggcgcga ctggcctgta cctcggcggc ggcttcccgg
      541 agacgttcgc ggagccactc gcggccaacc agccgctgcg tgccgccgtc cgcgcgttcg
      601 ccgcctccgg ccggccgctg ctggccgaat gcggcggcct gctctacctt gcccgcaccc
      661 tcgacgggca gccgatggcc ggggtggtgc cggccgacgc ccgcatgtcc gaccggctca
      721 ccctcggcta ccgccaggcg gtcgcggcag cgccgtcggt actgaccgcc gcgggcgagg
      781 cgctgcgcgg ccacgagttc cactactccc gcctcgaccc gcccgctggg acgccggcgg
      841 cctggctgct cggcgacgag cgccagccgg aggggttcac cgccgctggc gtccacgcga
      901 gctacctgca cacccactgg gcagccaccc ccgcgctggc cacccgcctg gtgagtgcgg
      961 ccgcatgacg ccgacgccag gaaccgcggc cggccgcctg atcggcgtgg gcgtcgggcc
     1021 tggcgatccc gagctgctca cggtcaaggc cgtccgcctg ctgcgcgagg ccgacgcggt
     1081 gttcgtcccg gtcgcggcga gcggcgagcc agggcgggcc gagaccgtgg tccgcgccta
     1141 cgtggacccg tcgcggatcc agtccgtcga gttcgccctc ggcgacgacc ctgccgcgcg
     1201 ccagacgaac tgggagcgtg cgggggaggc cgtggccgcg gtgctgcgcg ccggccaggt
     1261 agcggcgttc gcgaccatcg gcgatccgac cgtctactcg accttcgcct acctcgcggc
     1321 caccgtccgc aggctgctgc ccggggtgcg cgtcgagctg gtctccggca ttaccgccat
     1381 gcaggacctg gccgcccgca gcggcctcag cctggtggag gggagcgagc ggctcgccct
     1441 gctgcccttc ggtgccaacg ccgctgacct cgccgctttc gacacggtcg tctgctacaa
     1501 gggcggccgc cacctgcctg acctgctgga ggtcctgcgc cgcgcggggc ggctcgaccg
     1561 cgccctctac ggggcccgcc tcggcctggc cggcgaggtc gtgcgcccgg ccgccgagct
     1621 gcccgcccgc cccggcccct acctgtccac cctgatcgcc ctgcccgagc ggcggggaag
     1681 ggggtccgca ctgtgagcgc gaaggtgtgg ttcgtcgggg cggggcccgg cgcggcggac
     1741 ttgctcacct tcaggggcgc ggccgccatc gcagccgccg acgtcgtggt ctgggcgggc
     1801 agcctggtcg ccgccgaggt gctggtccac gcccgggccg gcgcggaggt gctcgactcc
     1861 tcggaccgga ccctcgagga ggtgacggcg gtctacgacc gcgcggtggc ggaggggctg
     1921 gtcgtcgcca gggtgcactc cggggacccg accctgtacg gcgcgatcca ggagcagatc
     1981 gccgagtgcg agcgtcgcgg gctcgcctgg gagatcgtgc cgggcgtgag ctcgatggcg
     2041 gccgccgccg ccgcggtcgg ccgcgagctg acgatcccgg gcgtcgccca gtcggtggtg
     2101 atcacccgga tggcgacccg cacgtccagc tcgatgccgc cgggagagcg ggtccgcgag
     2161 ctcgccgggc acggcgccac catggccctg ttcctgtcct gctcgcgccc gggagcgctg
     2221 cagcgggag
//
LOCUS       DASPYY010000449         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2042477, whole genome shotgun sequence.
ACCESSION   DASPYY010000449 DASPYY010000000
VERSION     DASPYY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042477"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>2111
                     /locus_tag="VEP73_07035"
     CDS             38..>2111
                     /locus_tag="VEP73_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYT26219.1"
                     /translation="MTTAADGSFSIGALRPGESYDIVTFMRDRPASRREGVGAPADGL
                     EIVLPSPGRISGRVVDAQTQTPVTDFQVSFAPDRSSGGGGFGPGGGGRGGGGGRAGLL
                     AALGGGRGDRQALHTDDGGFVLDDVPAGAWEVVVQAEGYQTARVGGITLDEGGKRDGV
                     DVRLRRGASIQGQVLDANSGQPVLDAAITLRRPGGAGAPALAADGDARTNSDGRFAVA
                     GLAAGSYTVMARHPDYADNSALVDVKDAPVTADLRLAPGGSVGGTVLSETNAPLPGAT
                     VTLGAGGGLGRGAAFAGGQTTISDDSGRFRFTHVTAGRYAVAASLRSRQTTPVELVLQ
                     AGEARENLVLSLGAGARIHGVVSGLPSSLRGNVNVSASGPDGYFTSARAATDGSYEVS
                     GAPAGPINLRATASDATGSTRSATSQVSIADGQVDLEADITFETGFTLSGTVTRAGQA
                     VADATVSAGLQGGGGRMASARTNDSGAYRLDGLLQGSYSVTAAAANGRPQRQTATISD
                     DTTLDFVLPSARLAGVVIEAGSQQPLPDATVQAAPQDAAGPAGRGAATDSNGRFSIDG
                     LEPQPYVVSARKVGYQVESKTVTPAEDGGGDVVLELVRGDGIGLEVRDAQFGVPVRAA
                     QARAVDAQGAVAFAGGVMLDGDGRGEIPSLLPGSYLLTLYPAGYAPAMVAATSPSPHV
                     LVAVSPGGA"
BASE COUNT          261 a          785 c          808 g          257 t
ORIGIN      
        1 cacgcgccgg ccggggcccg ccttcggcgg caacccgatg acgacggcgg ccgacgggtc
       61 cttctcgatc ggcgcactgc ggcccggcga gagctacgac atcgtgacgt tcatgcgcga
      121 ccgtccggcc tcgcgccgcg agggtgtggg agcccccgcc gacggcctcg agatcgtgct
      181 tccgagcccg ggccgcatct cgggaagggt cgtggacgcg cagacgcaga ctcccgtcac
      241 cgacttccag gtgtcgttcg cgccggaccg atcgagcggc ggaggcgggt tcgggcccgg
      301 tggcggcggc cgcggcgggg gcggagggcg ggccggcttg ctggccgccc tcggcggcgg
      361 gcggggtgac cggcaggccc tgcacaccga cgacggcggc ttcgtactcg acgacgtgcc
      421 cgccggagcg tgggaagtgg tggtccaggc cgagggctat cagaccgcgc gggtcggcgg
      481 catcacgctg gatgagggcg gcaagcgcga cggcgtggac gtgcggctcc gccgcggcgc
      541 atccatccag ggccaggtcc tggacgcgaa cagcggtcag ccggtcctcg acgccgccat
      601 cactttgcgg cgccccgggg gcgcgggcgc gcccgcgctc gcggcggacg gggatgcgcg
      661 cacgaacagc gacggtcgct tcgcggtggc cggcctggcc gcgggctcct acaccgtgat
      721 ggcccgccat cccgactacg ccgacaacag cgcgctcgtg gacgtgaagg acgcgcccgt
      781 taccgcggat ctgcggctcg cgcccggggg ctcggtgggc gggacggtcc tgtccgagac
      841 gaacgcgccg cttcccggcg cgaccgtgac gctcggtgcg ggcggtggcc tcggccgggg
      901 cgccgccttc gccggcggcc agacgacgat cagcgacgac tccggccgct tccgcttcac
      961 ccacgtgacg gccggccgct acgcggtcgc cgcttcgctg cgctcgcgcc agacgacacc
     1021 ggtcgagctg gtgctccagg ccggcgaagc gcgcgagaac ctcgtgctct cgctgggcgc
     1081 cggcgcccgc atccacggcg tcgtgagcgg gctgccgagc tcgctgcgcg ggaacgtcaa
     1141 cgtctcggcc agcggtcccg acggctattt cacctccgcg cgcgcggcga cggacgggtc
     1201 ctacgaggtg agcggcgcgc ccgccggccc gatcaacctg cgtgcgaccg ccagcgacgc
     1261 gacggggtcc acgcgctcgg ccacgagcca ggtctcgatc gccgacggcc aggtcgacct
     1321 ggaagccgac atcacgttcg agaccggctt cacgttgagc ggcaccgtga cccgagccgg
     1381 ccaggcggtg gcggacgcca cggtctccgc tggcctccag ggcggcggtg gccgcatggc
     1441 ctccgcccgc accaacgact cgggcgcgta ccggctcgat ggactgctcc agggctccta
     1501 ctccgtcacc gccgcggctg cgaacggccg gccccagcgc cagaccgcga cgatctccga
     1561 cgacacgacc ctggacttcg tgctgccctc ggcgcgcctg gccggcgtcg tgatcgaggc
     1621 gggatcgcag cagccgctgc ccgacgccac ggtgcaggcg gctccgcagg acgcggccgg
     1681 cccggccgga cggggcgccg ccaccgacag caacggccgc ttcagcatcg acgggctgga
     1741 gccgcagccg tacgtggtga gcgcgcgcaa ggtgggctat caggtcgaga gcaagaccgt
     1801 gacgccggcc gaggacggcg gcggcgacgt ggtgctggag ctggtcaggg gggacggaat
     1861 cgggctcgag gtgcgcgacg cgcagttcgg cgtgcccgtc cgggccgccc aggcccgcgc
     1921 ggtggacgcg cagggcgcgg tcgcctttgc cggcggcgtg atgctcgacg gcgacggccg
     1981 gggcgagatt ccgtcgctgc tcccgggaag ctacctgctc acgctgtacc ccgccggcta
     2041 tgcgccggct atggtcgccg ccacctcacc ctcgccgcac gtcctggtcg cggtgtcgcc
     2101 cggcggcgcc c
//
LOCUS       DASPYY010000450         6015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1761150, whole genome shotgun sequence.
ACCESSION   DASPYY010000450 DASPYY010000000
VERSION     DASPYY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761150"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VEP73_07040"
     CDS             <1..267
                     /locus_tag="VEP73_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HYT26220.1"
                     /translation="GADSPDILVFSKRTGDDAVLVVVNLDPWNTHDSIVHLWLPMLGL
                     DWDSGAFDVHDELTGTTWTWAGPTNYVRLDPSHEPAHVLHVRRR"
     gene            274..1686
                     /locus_tag="VEP73_07045"
     CDS             274..1686
                     /locus_tag="VEP73_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYT26221.1"
                     /translation="MTELEAALAEFLPRQRWFAGKGRSVVEVRTEASVPLRTPLPSLH
                     MVVAGVAYADGGVERYSIPMGHDRGPEARQLSSRHREAVILETAPPQSGIFYDAVADR
                     RLGSVFLDHLNKGATLDEVRFNKRPTWSDKLRGPGRLLDAEQSNSSLVFGDRLILKLF
                     RRLQPGINPELELTRALTAKGFAACPAPLGWIEGLGSTLGILQPFYADSVEGWRLATE
                     RVADHYETGSAAGNFATEARELGLVTADMHSALAGALPMVASAQPDLGHLSARLLGQL
                     TQVAALVGELRPYKDAIEGVYAKAEAAGGARHLQRIHGDYHLGQVLRIRGQAVASGEA
                     QSWMIIDWEGEPARNFEERRRLASPLQDAAGMLRSFDYAASQPLVLGEDPDTPGPSRE
                     ELEGLERTGAAWVEANRAAFLDGYLGRARDAGWLPGDHEVLLRTFELDKAVYEVMYEA
                     RHRPGWLSIPLGGIRRLLGS"
     gene            1691..3868
                     /gene="glgB"
                     /locus_tag="VEP73_07050"
     CDS             1691..3868
                     /gene="glgB"
                     /locus_tag="VEP73_07050"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYT26222.1"
                     /translation="MPTPTLARQEIERLVRGEHHEPHRLLGAHPAGGQVAIRVYRPDA
                     TEVTALLDGDGERVKLTQIHPAGLFEGLVDGERVPAYRLEVAYGDRTFTVDDPYRFLP
                     TIGAIDEHLFSEGTHERLWGKLGAHERVLDGVAGTSFAVWAPNARAVRVVGDFNSWDG
                     RLHPMRSIGASGIWELFAPGVAAGARYKYEIVTSRGDLVLKADPFAFATEKPPATASV
                     VTTSGHEWGDRDWMASRDQRHKRNGPVSVYEVHIGSWRRRDGSAFLTYRELAAELPVY
                     VADLGFTHVEFMPVAEHPYGASWGYQVTGYYAPTARYGTPDDFRYLVDRLHQRGIGVI
                     VDWVPAHFPKDAFALARFDGTALYEHDDPRRGEHPDWGSLVFNFGRNEVRNFLIANAL
                     FWLEEYHVDGLRVDAVASMLYLDYSREDGEWTPNQYGGNEDLDAVQFLKDFNTVVYRE
                     HPGAVTVAEESTAWSGVSRPVYLGGLGFGFKWNMGWMHDTLEYIAKDPIYRRFQHHQL
                     TFSLVYAFSENFVLPISHDEVVHGKRSLLGKMPGDTWRRFANLRAYLAFMWAHPGKQL
                     IFMGSELAQESEWSNERSIDWDALADPHHRGVQDLVRDLNRTYLAHPALYERDAEPAG
                     FGWIDPNDTDDNALSFIRWSASDEPLVCLCNFSPVVREGYRVGLPRTGRWKEVLNTDA
                     EAYGGSNVGNLGLVESEAIGWDGQPASARVTLPPLATVWLAPA"
     gene            3865..5667
                     /gene="treZ"
                     /locus_tag="VEP73_07055"
     CDS             3865..5667
                     /gene="treZ"
                     /locus_tag="VEP73_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HYT26223.1"
                     /translation="MTARAAPGGGAADADAGPGARRTPPLGPRLLAGGAEFSVWAPRA
                     ASLAVRHGGRTVPLAREERGVWRGTVPGARAGDDYLLVLDGRLARPDPRSRLQPQGPH
                     GPSRLVDLGGPPARFAPPALRELVFYELHCGTFTPEGTFDAAAARLGHLVDLGVTAVE
                     VMPVAAFPGRRGWGYDGVDLYAVHAAYGGPAAFRRFVDACHAVGLAVVLDVVYNHLGP
                     DGNYLAEFGPYFTDRYRTPWGPALNYDGPGSDFVRDFVLDNAEQWVAGYGVDGLRLDA
                     VHEIHDRSSVHLLEALADRVHGRAPGGVLVAESDLNQPALVGSYGIDTQWADDFHHAV
                     HAALTGERDGWYRGYLGLGQLAKALTRGWVFTGQYDPAADRTRGREPSGLGGERFVVC
                     SQNHDQVGNRAFGDRLAALVGPRLDAVASVLVACSPYLPLLFQGQEWGETRPFEYFTD
                     HGPALAEAVRRGRRAEFAAFAWSGAVPDPNDPGSFERSRLDWAKADAPAPEGLALPVL
                     ELWRRLLRLRREVPALGNCRRDLARAWADEERRLVVLERDDPSGSRAVVVANLSGEPR
                     PLPAAARGLPQLLTTASGASSEEVPGHGSVVHGS"
     gene            5737..>6015
                     /locus_tag="VEP73_07060"
     CDS             5737..>6015
                     /locus_tag="VEP73_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26224.1"
                     /translation="MSADIEIERFGLGRLERLDLRSFWGDQVPDLMPWFAQNLDLLSQ
                     TLGIEITPLQRDPHVDRTPMQVLGTDAHGRPVMVENRLEPTEQSHLGQL"
BASE COUNT          813 a         2286 c         2101 g          815 t
ORIGIN      
        1 ggcgccgact cgcccgacat cctggtgttc tccaagcgga ccggcgacga cgccgtcctc
       61 gtcgtcgtca acctcgaccc ctggaacacc cacgacagca tcgtccacct gtggctcccc
      121 atgctcggcc tcgactggga cagcggcgcg ttcgacgtcc acgacgagct gaccggcacg
      181 acctggacct gggcaggccc caccaactac gtccgcctcg acccctccca cgagccagcg
      241 cacgtcctcc acgtccggcg aaggtaagcc gccatgaccg agctcgaggc cgccctggcc
      301 gagttcctgc cccgccagcg ctggttcgcc ggcaagggcc gttcggtggt ggaggtccgc
      361 accgaggcgt cggtgccgct gcgcaccccg ctgcccagcc tgcacatggt cgtcgccggg
      421 gtggcgtacg ccgacggcgg ggtcgagcgg tactccatcc ccatgggcca cgaccgcggc
      481 ccggaggccc gccagctctc gagccgccat cgtgaggccg tcatcctcga gaccgcgccg
      541 ccccagtccg ggatcttcta cgacgcggtc gccgacaggc gcctcggctc ggtcttcctc
      601 gaccacctga acaagggcgc cacgctcgac gaggtgcgct tcaacaagcg ccccacctgg
      661 tcggacaagc tccgcggccc cggcaggctg ctcgacgccg agcagtcgaa ctcctcgctg
      721 gtgttcggcg accggctcat cctcaagctg ttccgccgcc tccagccggg gatcaacccg
      781 gagctcgagc tgacccgcgc gctgaccgcc aaggggttcg cggcctgccc ggcgccgctc
      841 ggctggatcg aggggctcgg ctcgacgctc gggatcctgc agcccttcta cgcggacagc
      901 gtggaggggt ggcggctcgc caccgagcgg gtcgccgacc actacgagac gggctcggcc
      961 gccggcaact tcgccaccga ggcgcgcgag ctcggcctgg tcaccgccga catgcacagc
     1021 gcgctggccg gcgcgctgcc gatggtggcc tcggcgcagc cggacctcgg ccacctctcg
     1081 gcccgcctgc tcggccagct cacccaggtg gccgcgctgg taggagagct ccggccctac
     1141 aaggacgcga tcgagggcgt gtacgccaag gccgaggcgg ccgggggcgc gaggcacctc
     1201 cagcgcatcc acggcgacta ccatctgggc caggtgctgc gcatccgcgg ccaggcggtg
     1261 gcgagcggcg aggcccagtc ctggatgatc atcgactggg agggggagcc cgcccggaac
     1321 ttcgaggagc gccgccggct cgccagcccg ctgcaggacg cggccggtat gctgcgctcc
     1381 ttcgactacg ccgcgagcca gccgctcgtg ctcggcgagg accccgacac cccgggcccg
     1441 agccgggagg agctcgaggg gctcgagcgg accggggcgg cctgggtcga ggcgaaccgg
     1501 gccgcgttcc tggacggcta cctgggccgg gcgagggacg ccggctggct gccgggcgac
     1561 cacgaggtgc tgctgcgcac gtttgagctc gacaaggcgg tctatgaggt gatgtacgaa
     1621 gccaggcacc gcccggggtg gctctccatc ccgctcggtg gcatccgccg actgctcggg
     1681 agctgaccac atgccaaccc ccaccctcgc caggcaggag atcgagcgtc tcgtccgcgg
     1741 cgagcaccac gagccgcacc gcctgctcgg ggcgcacccg gccggcggcc aggtcgcgat
     1801 ccgggtctac cgacccgacg cgaccgaggt gaccgccctg ctcgacgggg acggcgagcg
     1861 ggtgaagctc acccagatcc acccggccgg cctgttcgag ggcctggtgg acggcgagcg
     1921 ggtgcccgcc taccggctcg aggtcgccta cggcgaccgc accttcaccg tcgacgaccc
     1981 ctaccggttc ctgcccacga tcggggcgat cgacgagcac ctgttcagcg agggcaccca
     2041 cgagcggctg tgggggaagc tcggcgccca cgagcgcgtc ctggacggcg tggccggcac
     2101 gtcgttcgcg gtctgggcgc ccaacgcccg cgccgtgcgg gtcgtgggcg acttcaacag
     2161 ctgggacggg cgcctgcacc cgatgcggtc gatcggggcg tccggcatct gggagctgtt
     2221 cgcgcccggg gtcgcggcgg gggccaggta caagtacgag atcgtcacct cgcgcggcga
     2281 cctcgtcctc aaggcggacc cgttcgcgtt cgccaccgag aagccgccgg ccaccgcgag
     2341 cgtcgtcacc acctcggggc acgagtgggg cgacagggac tggatggcat cccgcgacca
     2401 gcgccacaag cgcaacggcc cggtctcggt ctacgaggtg cacatcgggt cgtggcgccg
     2461 tcgcgacggc agcgcatttc tcacttatcg cgagctggcc gccgagcttc ccgtgtacgt
     2521 cgccgacctg ggcttcaccc acgtcgagtt catgcccgtc gccgagcatc cctacggcgc
     2581 ctcgtggggc taccaggtca ccggctacta cgcgccgacg gccaggtacg ggaccccgga
     2641 cgacttccgc tacctggtcg accggctcca ccagcgcggg atcggggtga tcgtcgactg
     2701 ggtgcccgcc cacttcccca aggacgcctt cgcgctcgcc cgcttcgacg gcaccgccct
     2761 gtacgagcac gacgacccgc gccgggggga gcaccccgac tggggctcgc tggtgttcaa
     2821 cttcggccgc aacgaggtgc gcaacttcct catcgccaac gccctgttct ggctcgagga
     2881 gtaccacgtc gacgggctgc gcgtggacgc ggtcgcctcg atgctctacc tcgactactc
     2941 gcgcgaggac ggcgagtgga cgccgaacca gtacggcggc aacgaggacc tcgacgcggt
     3001 ccagttcctc aaggacttca acaccgtcgt gtaccgggag cacccgggcg cggtcaccgt
     3061 cgccgaggag tcgaccgcct ggtccggcgt gtcccgcccc gtctacctgg gcggcctcgg
     3121 gttcgggttc aagtggaaca tgggctggat gcacgacacg ctcgagtaca tcgccaagga
     3181 cccgatctac cggcgcttcc agcatcacca gctcaccttc tcgctggtgt acgcgttcag
     3241 cgagaacttc gtgctgccga tctcgcacga cgaggtggtg cacggcaagc gctcgctgct
     3301 cggcaagatg ccgggcgaca cctggcgccg gttcgccaac ctgcgcgcct acctcgcgtt
     3361 catgtgggcc catcccggca agcagctcat cttcatgggc tccgagctgg cccaggagtc
     3421 cgagtggtcc aacgagcggt cgatcgactg ggacgccctc gccgatcccc accaccgcgg
     3481 cgtccaggac ctcgtccgcg acctgaaccg cacctacctc gcccacccgg ccctgtacga
     3541 gcgcgacgcc gagccggccg ggttcggctg gatcgacccc aacgacaccg acgacaacgc
     3601 gctctcgttc atccgctgga gcgcgagcga cgagccgctc gtctgcctct gcaacttctc
     3661 gccggtggtc agggagggct accgggtcgg cctgccgagg accggccggt ggaaggaggt
     3721 cctgaacacc gacgcggagg cctacggcgg ctccaacgtc ggcaacctcg gcctcgtcga
     3781 gagcgaggcg atcggctggg acggccagcc cgcctcggcg cgggtgacgc tgccgccgct
     3841 ggcaacggtc tggctcgccc cggcgtgacg gcccgtgcgg cccccggggg tggggccgcg
     3901 gacgcggacg ccggcccggg cgcccgcagg acgccgccgc tcgggccgag gctcctggcc
     3961 ggcggggccg aattcagcgt ctgggcgccc agggcggcct ccctcgccgt ccgccacggc
     4021 ggccggaccg tccccctcgc gcgcgaggag cgcggggtct ggcggggcac ggtcccaggc
     4081 gcccgcgccg gcgacgacta cctgctcgtg ctcgacgggc ggctcgcgcg gcccgacccg
     4141 cgctcgcggc tgcagcccca ggggccgcac gggccgtcgc gcctggtcga cctcggcggc
     4201 ccgcccgccc ggttcgctcc ccccgccctt cgcgagctcg tcttctacga gctccactgc
     4261 ggcaccttca cgccggaggg gaccttcgac gcggccgccg cgaggctcgg ccacctcgtc
     4321 gacctcgggg tgaccgcggt cgaggtgatg ccggtggcgg ccttccccgg gcggcgcggc
     4381 tggggctacg acggcgtgga cctgtacgcg gtgcacgccg cctacggcgg cccggccgcc
     4441 ttccggcgct tcgtcgacgc ctgccacgcc gtggggctcg ccgtcgtcct cgacgtggtc
     4501 tacaaccacc tcggaccgga cgggaactac ctcgccgagt tcggcccgta cttcaccgac
     4561 cgctaccgga ccccctgggg accggcgctc aactacgacg gcccaggctc cgacttcgtg
     4621 cgcgacttcg tcctcgacaa cgccgagcag tgggtcgccg ggtacggggt cgacgggctc
     4681 cgcctcgacg ccgtgcacga gatccacgac cgctcctcgg tgcacctgct cgaggccctc
     4741 gccgaccggg tccacgggcg cgcgcccggc ggcgtgctcg tggccgagag cgacctcaac
     4801 cagcccgccc tggtcggctc gtacgggatc gacacgcagt gggccgacga cttccaccac
     4861 gccgtccacg ccgccctgac cggggagcgc gacggctggt accgcgggta cctgggcctc
     4921 gggcagctcg ccaaggccct cacccgcggg tgggtgttca ccggccagta cgacccggcc
     4981 gcggaccgga cgcgcggccg ggagccgtcc gggctcggcg gtgagcgctt cgtggtgtgc
     5041 tcccagaacc acgaccaggt cggcaaccgg gccttcggcg accggctcgc ggccctggtc
     5101 gggcccaggc tggacgcggt cgcgagcgtc ctggtcgcct gctcgcccta cctcccgctg
     5161 ctcttccagg gccaggagtg gggcgagacc aggccgttcg agtacttcac cgaccacggg
     5221 cccgcgctgg cggaggccgt gcgccgcggc cgccgggccg agttcgccgc cttcgcctgg
     5281 tccggcgccg tgcccgaccc gaacgacccg gggtcgttcg agcgttcgag gctcgactgg
     5341 gccaaggcgg acgcgccggc gcccgagggg ctcgcgctgc cggtgctcga gctgtggcgg
     5401 cgcctgctcc ggctccgccg cgaggtcccc gccctcggca actgccggcg ggacctcgcg
     5461 cgggcgtggg ccgacgagga gcggcggctg gtcgtcctcg agcgggacga cccgtcgggc
     5521 tcgcgggcgg tcgtcgtcgc caacctctcc ggggagccgc ggccgctgcc ggccgccgcg
     5581 cgcgggcttc cacagctgct gaccacggca tcgggcgcat cctcggagga ggtccccggc
     5641 cacggttcag tagttcatgg tagttgaccg gtttgacgtc ggcccgtccg aaggaggtac
     5701 aactggcatc cgacgggggc cagcgagagg agcggaatgt cggccgacat cgaaatagag
     5761 agatttggcc tcggtcgtct cgaacggctc gacctccgtt cgttctgggg cgaccaggtg
     5821 cccgacctca tgccctggtt cgcgcagaac ctggacctcc tcagccagac cctcgggatc
     5881 gagatcacgc cgctgcaacg cgacccgcac gtggaccgca cccccatgca ggtgctcggc
     5941 accgacgcgc acggccgtcc cgtgatggtc gagaaccggc tggagccgac cgagcagagc
     6001 cacctcgggc agctc
//
LOCUS       DASPYY010000451         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1620832, whole genome shotgun sequence.
ACCESSION   DASPYY010000451 DASPYY010000000
VERSION     DASPYY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620832"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VEP73_07065"
     CDS             complement(<1..562)
                     /locus_tag="VEP73_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT26225.1"
                     /translation="MGVEDWLAAAARFGARFRERYEPYEPHPALAVDEGRLAAAWAEY
                     TERLEDNYPFFHPRYAGQMLKPPHPVAVAGYLAAMLVNPNNHALDGGPATGAMEKEVV
                     ADLAGMFRLPEGTLGHLTSSGTIANLEALWVARQLHPGRAVAFCEEAHYTHERMCGVL
                     GVETVKVPADDSGRMDLDALEEALATG"
     gene            complement(586..1482)
                     /locus_tag="VEP73_07070"
     CDS             complement(586..1482)
                     /locus_tag="VEP73_07070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYT26226.1"
                     /translation="MTTALEPEGLRHLASGKVRDLYEADAGHLLVVASDRISAFDVVL
                     EDPVPGKGVVLTALTQFWLEQLGDLVANHLVGWRASELPSGARQLAGRAMLVRRLEMV
                     PIECVARGYLVGSGWKEYQAGGTVCGIPLPAGLRESERLPAPIFTPATKAVTGHDENV
                     SEARMAELVGTALTTRLRHLTVEVYRHAAEHAAKRGILLADTKLEFGLADGEVVLADE
                     VLTPDSSRFWPAGAWEPGQSPPSFDKQYVRDWLEAQAWDKTPPGPALPPDVVAGTAAR
                     YREAYERLSGRTLADWLAEARS"
     gene            complement(1532..2830)
                     /gene="purB"
                     /locus_tag="VEP73_07075"
     CDS             complement(1532..2830)
                     /gene="purB"
                     /locus_tag="VEP73_07075"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYT26227.1"
                     /translation="MIPRYVLPEMGAVWSEAERFRLSARVEAAAVRARARRGLVPAAD
                     LDAVEGAPAPSPERVHELDARLHHDVVAFLTAYGEGMGPAARHVHYGMTSSDLLDTAL
                     ALQLTRACDLLAGRLDRLVAVLRDRAREHRDTLCLGRTHGVAAEPTTFGLKLAGHAFA
                     FDRDRRRLAAAREAVAVGTVSGAVGTYANLPADVEAEVLAELGLRPEDAPTQVVARDR
                     HAELLATLAVAGAGLERLAVEVRHLQRTEVREAEEPFAEGQKGSSAMPHKRNPIVAER
                     LTGVARLLRGYAVAGLEDVALWHERDISHSSVERVVLPDACCVLDYGLERAAWLVEGL
                     RVFPERMRANLEASGGLAGSSAALLALVDADWSREDAYAAVQRAAMAAWEGKGGFRDL
                     LLAEPGVADVLGGHALDEALDPARFVANAGPVFDRLEALP"
     gene            complement(2865..3332)
                     /gene="purE"
                     /locus_tag="VEP73_07080"
     CDS             complement(2865..3332)
                     /gene="purE"
                     /locus_tag="VEP73_07080"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYT26228.1"
                     /translation="MPAPRVGIVLGSSSDEEKAKAASVMLEKFGVDYELEVASAHRSP
                     RRVADWAASAEDRGLQVLIAAAGRSAALPGVVAAHTTLPVIGLPIFTPHLGGADALYS
                     IVQMPAGVPVATVGLDAAANAGILAVQILAVGDPGLRKQLRDFKHQLSEGLRL"
     gene            complement(3451..>4033)
                     /locus_tag="VEP73_07085"
     CDS             complement(3451..>4033)
                     /locus_tag="VEP73_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYT26229.1"
                     /translation="TVTANLSMIVAQARVAERVLPVGNAPPMDAIDFIRKTGEQTLAE
                     LRRTADVLRPEEPVVVPASTHSAMSQLGALVAEVKMAGLPVDVVVEGEARPLPAQLDG
                     SAYRIVQEALSNCLKHPGPVRPRLLIRYGADDLVLEVTDDGGGGSWEVDPEGGRRENL
                     GWMQPRASAGPTATATAEHWLVGAYGVRARLPL"
BASE COUNT          507 a         1481 c         1532 g          513 t
ORIGIN      
        1 gcccggtcgc gagcgcctcc tcgagcgcgt cgaggtccat ccgacccgag tcgtccgcgg
       61 gcaccttgac cgtctccacc ccgaggacgc cgcacatgcg ctcgtgggtg tagtgggcct
      121 cctcgcagaa tgcgaccgcc ctgcccgggt ggagctgccg cgccacccag agggcctcga
      181 ggttggcgat cgtgccgctc gaggtgaggt ggccgagggt gccctcggga agccggaaca
      241 tcccggccag gtcggcgacg acctccttct ccatcgctcc ggtggccgga ccgccgtcca
      301 gggcgtggtt gttggggttg accagcatcg cggccaggta gccggcgacc gccaccgggt
      361 gtggcggctt cagcatctgg cccgcgtacc tgggatggaa gaacgggtag ttgtcctcga
      421 ggcgctcggt gtactccgcc caggcggccg cgagccggcc ctcgtcgacg gccagcgccg
      481 ggtggggctc gtagggctcg taccgttccc ggaaccgcgc gccgaaccgc gcggccgccg
      541 ccagccagtc ctcgacgccc atcgccgctg cctcgcgccg tgcggtcagg accgcgcctc
      601 ggcgagccag tcggccagcg tacggccgga caggcgctcg taggcctcgc ggtaccgcgc
      661 cgcggtgccc gcgaccacgt cggggggcag ggcggggcct ggcggggtct tgtcccatgc
      721 ctgggcctcg agccagtcgc gcacgtactg cttgtcgaac gagggcggcg actggcccgg
      781 ctcccaggcg cccgccggcc agaagcgcga cgagtcgggt gtgagcacct cgtcggccag
      841 gacgacctcg ccgtcggcga gaccgaactc gagcttcgtg tcggcgagca ggatcccccg
      901 cttcgccgcg tgctcggccg cgtggcggta cacctcgacg gtgaggtggc ggagccgggt
      961 ggtcagcgcc gtcccgacca gctcggccat gcgggcctcg gagacgttct cgtcgtggcc
     1021 ggtgaccgcc ttggtggcgg gggtgaagat cggcgcgggc agccgctcgg actcccgcag
     1081 gcccgccggg agcgggatcc cgcagacggt gccgcccgcc tggtactcct tccagcccga
     1141 cccgaccagg tagccgcggg cgacgcactc gatcgggacc atctcgagcc ggcgcaccag
     1201 catggcccgg ccggcgagct gtcgcgcccc ggacggcagc tcggaggcac gccagccgac
     1261 caggtggttg gcgaccaggt cgccgagctg ctcgagccag aactgggtga gcgcggtgag
     1321 caccaccccc ttgcctggca ccgggtcctc gagcacgacg tcgaacgcgg agatgcggtc
     1381 cgacgcgacg accagcaggt ggcccgcgtc ggcctcgtag aggtcgcgca ccttgcccga
     1441 ggcgaggtgg cgcaggccct cgggctcgag ggcggtggtc atcggcactc ccttctcgaa
     1501 gttgcgctgg tctcgggtac gtccgccggt gtcacgggag cgcctccagg cggtcgaaga
     1561 ccgggccggc gttggcgacg aaccgcgccg ggtcgagcgc ctcgtcgagg gcgtgcccgc
     1621 cgagcacgtc cgcgacaccc ggctcggcga ggagcaggtc ccggaagccg cccttgccct
     1681 cccaggcggc catggccgcc cgctggaccg cggcgtacgc atcctccctg ctccagtcgg
     1741 cgtcgaccag ggcgaggagg gcggcagagg agccggcgag gccaccggac gcctcgaggt
     1801 tcgcccgcat ccgctcgggg aagacgcgca atccctcgac cagccaggcg gcacgctcca
     1861 gaccgtagtc cagcacgcag caggcgtcgg ggagcacgac ccgctcgacc gacgagtggg
     1921 agatgtcccg ttcgtgccat agggcgacgt cctcaagccc ggccacggcg tacccgcgga
     1981 gcagccgggc gaccccggtg agacgctcgg ccacgatcgg gttgcgcttg tggggcattg
     2041 ccgacgaccc cttctggccc tccgcgaacg gctcctcggc ctcccggacc tcggtgcgct
     2101 gcaggtggcg gacctcgacc gccagccgct cgaggccggc ccccgcgacg gcgagcgtgg
     2161 cgagcagctc ggcgtggcgg tcgcgggcga ccacctgggt gggggcatcc tccgggcgga
     2221 gcccgagctc ggcgaggacc tcggcctcga cgtcggcggg caggttggcg tacgtgccga
     2281 ccgcccccga gaccgtcccc accgcgaccg cctcgcgggc ggcggcgaga cggcgccggt
     2341 cgcggtcgaa cgcgaaggcg tgcccggcga gcttcagccc gaaggtcgtc ggctcggcgg
     2401 cgacgccgtg ggtgcggccg aggcagaggg tgtcgcggtg ctcccgggcg cggtcgcgca
     2461 gcacggccac cagccggtcg agccggccgg cgagcaggtc gcaggcgcgg gtgagctgga
     2521 gcgcgagcgc ggtgtcgagc aggtcggagg aggtcatgcc gtagtggacg tgcctggccg
     2581 ccgggcccat gccctccccg taggcggtga ggaaggcgac cacgtcgtgg tggagccgcg
     2641 cgtcgagctc gtggacgcgt tcgggggacg gcgccggggc gccctcgacg gcgtcgaggt
     2701 cggcggccgg caccagcccg cgcctggccc gggcgcggac cgccgccgcc tcgacccgcg
     2761 cggacaggcg gaaccgctcg gcctcgctcc agacggcgcc catctcgggg aggacgtagc
     2821 gagggatcac gcttgaccct ttcggctcct gcccagcggg cgcgctacag gcgcaggccc
     2881 tccgagagct ggtgcttgaa gtccctgagc tgcttgcgca gcccagggtc gccgacggcg
     2941 aggatctgca cggcgaggat ccccgcgttc gccgccgcgt ccagccccac cgtggccacc
     3001 ggcacgccgg ccggcatctg gacgatcgag tagagggcgt ccgcgccgcc caggtggggc
     3061 gtgaagatgg gcaggccgat gaccgggagc gtggtgtggg cggcgacgac gccggggagc
     3121 gccgccgacc ggccggccgc ggcgatgagg acctggaggc cgcggtcctc ggcgctcgcg
     3181 gcccagtcgg cgacccgccg cgggctgcgg tgggcgctgg ccacctcgag ctcgtagtcg
     3241 accccgaact tctccagcat gacggaggcg gccttggcct tctcctcgtc ggagctggag
     3301 ccgaggacga tcccaaccct tggcgctggc atgcccactc ctcacgcgct gcactggcga
     3361 ccggcgcatt ctatgccgcc cgcgccaccc gggaccgtca cggcgctccg ggctcagagc
     3421 ggcagccgct ccgggctggc aaggggcgtc tcacagcggg agccgggcgc gcacgccgta
     3481 ggcgcccacg agccagtgct cggccgtggc cgtggcggtc ggcccggcgc tcgcccgcgg
     3541 ctgcatccag ccgaggttct cgcgccggcc gccctcagga tcgacctccc agctcccccc
     3601 gccaccgtcg tcggtgacct cgagcacgag gtcgtcggcg ccgtagcgga tcagcagccg
     3661 gggtcggacc gggccggggt gcttgaggca gttgctcagc gcctcctgca cgatccggta
     3721 ggccgacccg tccagctggg cggggagtgg gcgcgcttcc ccctcgacga cgacgtcgac
     3781 ggggagccca gccatcttga cctcggccac gagggctccg agctggctca tggcggagtg
     3841 cgtggacgcc gggacgacca ccggctcctc cggccgcagc acgtcggcgg tccggcgcag
     3901 ctcggcgagg gtctgctcgc ccgtcttgcg gatgaagtcg atggcgtcca tcggcggcgc
     3961 gttgccgacg ggcaggaccc gctcggccac cctggcctgg gcgacgatca tgctcaggtt
     4021 ggccgtgacc gtc
//
LOCUS       DASPYY010000452         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2042877, whole genome shotgun sequence.
ACCESSION   DASPYY010000452 DASPYY010000000
VERSION     DASPYY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042877"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEP73_07090"
     CDS             <1..259
                     /locus_tag="VEP73_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26230.1"
                     /translation="RSGARGRVEGLTMRTEPVADGVARVVLTGGTLVPSGVAGARPEP
                     LPKDGHAAVVVVERDGAWYPSGLFSAVDALIASAEREQGGG"
     gene            308..709
                     /locus_tag="VEP73_07095"
     CDS             308..709
                     /locus_tag="VEP73_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26231.1"
                     /translation="MTKILLVASQSSFRDRLRLALERLEASGDEIEFLDAANGNDAIA
                     IAEAEFPDLVVVEVGVTPYGGFGLTRDLKAVPDLACPVIIILERMQDEWLGRWCGADA
                     MVNQPVDPFALTGAARRLLEEDDAALEEATS"
     gene            706..999
                     /locus_tag="VEP73_07100"
     CDS             706..999
                     /locus_tag="VEP73_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26232.1"
                     /translation="MSIAAHCGSCGRELLLEQLTDPAEGFRCPFCGFAFAPSYATVAP
                     AIAVRALAAREALAGSLAELQSMIGDRLRLDRQSVLAAVTDALPASGQPVKTG"
     gene            1133..1936
                     /locus_tag="VEP73_07105"
     CDS             1133..1936
                     /locus_tag="VEP73_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26233.1"
                     /translation="MASQDRSRIGHGSGLPVTGRGGNGARPVASRPLRLDQPARPPPR
                     HLASSRRLPAPGLARSMLSAGLLVMLVLALGGTVVLRALSRPGVPGLQRGRPPVASRT
                     TAASVVPSTTLRARAAVVQSLVPDGSFEAGLAVMRDRPGTSCTRVAGGAAGRWSMQIR
                     ALGGAPVPPGLWIDLARGAPPGTRYTAWAAVRGRPGLTAQIRLYERGPGGAIADRTLV
                     VLHDLGWHQLGVKHAVAAPGSVLAVDVLIGGLAPGQTAQIDNVRAVRAP"
     gene            2055..2543
                     /locus_tag="VEP73_07110"
     CDS             2055..2543
                     /locus_tag="VEP73_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26234.1"
                     /translation="MRIRRVLPIGLVATLLWLSGGAAHAVQLLSVADVTCGGLSVTGT
                     GLPADTLLVLAVRQHPLGPAVRSLTVRTTAEGTARADVHASFAGFDEVSVAALDAAGR
                     RTLVIASHQFARPCPAEQPVLPVTGAAQDRPWLLATGLGLVVVGIGVRARTAYRGTHA
                     RR"
     gene            2572..2952
                     /locus_tag="VEP73_07115"
     CDS             2572..2952
                     /locus_tag="VEP73_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26235.1"
                     /translation="MLEEWDLWFPGAAATGISFARSRVEAGAAGDRLLVHAAPPKLAV
                     TVRAEDGQVLAEGHDLERGEPGPMSYLVRQGDAIRLEDGWPTQDDLGRLVILPGGEAG
                     VLTGWWHADDRSEWRWQIELYNHV"
BASE COUNT          365 a         1142 c         1123 g          397 t
ORIGIN      
        1 gcggtccggc gcccgggggc gggtcgaggg cctgaccatg cggaccgagc cggtggccga
       61 cggcgtcgcc cgggtcgtgc tgaccggcgg cacgctggtc ccgagcgggg tcgcgggggc
      121 caggcccgag ccgctgccga aggacgggca cgccgccgtc gtcgtggtcg agcgggacgg
      181 ggcgtggtac ccgtccgggc tgttcagcgc ggtcgacgcg ctgatcgcca gcgccgaacg
      241 ggagcagggg ggcgggtaga ctccatactc cgatgcacca cacgtccatc cctggtcggg
      301 agcgcccgtg acgaagatcc tgctcgtcgc ctctcaatcg tccttccggg accggctgcg
      361 cctcgccctc gaacgcctcg aggcgagcgg ggacgagatc gagttcctcg atgcggccaa
      421 cggcaacgac gccatcgcca tcgccgaggc ggagttcccc gacctcgtgg tcgtggaggt
      481 cggcgtgacg ccgtacggcg gcttcggcct gacgcgggac ctcaaggcgg tgccggacct
      541 cgcctgcccc gtcatcatca tcctcgagcg catgcaggac gagtggctcg gccgctggtg
      601 cggcgccgac gcgatggtca accagcccgt cgaccccttc gccctcaccg gggcggcccg
      661 ccgcctgctc gaggaggacg acgccgccct cgaggaggcc acctcatgag catcgccgcc
      721 cactgcggct catgcggtcg ggagctgctg ctcgagcagc tcaccgaccc ggccgagggg
      781 ttccggtgcc ccttctgcgg gttcgctttc gccccgtcgt acgcgaccgt cgcgcccgcg
      841 atcgccgtgc gggcgctcgc cgcccgggaa gcgctggccg gctcgctcgc cgagctgcag
      901 tcgatgatcg gcgaccgcct ccggctcgac cggcagagcg tcctcgccgc cgtgaccgac
      961 gcgctcccgg cctccggtca gcccgtcaaa accggctgac ggcgagaatc cacccagatc
     1021 gcatcgaacc ggccgcaact gggggatagc gttggccttc gcgggatagt agcgttgcgt
     1081 gggcagcggc gcggtaggcg cgatgcaggg ggtcttggag ggagaccttt ccatggcgag
     1141 tcaggaccgt agccggatcg gccacggctc cggcctcccg gtgacggggc gcggcggcaa
     1201 cggcgcccgg ccggtcgcca gtcgacccct ccgcctcgac cagcccgccc ggccgccgcc
     1261 gcggcatctc gcctccagcc ggcgcctgcc cgcgcccggc ctcgcccggt cgatgctgtc
     1321 cgccggcctc ctcgtgatgc tcgtcctcgc cctgggcgga acggtcgtcc tccgcgccct
     1381 cagccggcca ggcgtgccgg ggctgcaaag gggaaggccg cccgtggcgt cccggacgac
     1441 cgccgccagc gtcgtgccgt cgaccacgct gcgggcgagg gcggccgtcg tccagtccct
     1501 ggtccccgac ggcagcttcg aggccggcct ggccgtcatg cgggaccggc cgggtaccag
     1561 ttgcacacgc gtggccggtg gcgccgcggg ccgctggtcg atgcagatcc gggcgctggg
     1621 cggggcgccg gtgccgcccg gcctctggat agacctcgcc cgcggcgccc cgccgggcac
     1681 gcgctacacc gcctgggccg cggtacgcgg ccgacccggc ctgaccgccc agatccggct
     1741 ctacgagcgc ggccccggcg gcgcgatcgc cgaccggacc ctggtcgtcc tgcatgacct
     1801 gggctggcac cagctgggcg tcaagcacgc ggtggcggct cccggctcgg tgctcgccgt
     1861 cgacgtgctc atcggcgggc tcgcgcccgg gcagaccgcc cagatcgaca acgtgcgagc
     1921 cgtgcgggcc ccgtagcggc gcgccaccgg accgccggac gcagggagca ggtttggccg
     1981 gtcccgcaac cgccgatcat tgctgaggac gcgccgttcg ccattgacgc gcgggcgagg
     2041 ggatggagga cggggtgagg atccggcggg tgctgccgat cgggctcgtc gcgacgctcc
     2101 tgtggctctc cgggggcgcg gcccatgccg tgcagctgct gtcggtcgcc gacgtgacct
     2161 gcggcgggct cagcgtgacg gggaccgggc tccccgcgga cacgctgctg gtcctggcgg
     2221 tcaggcagca cccgttgggt cccgcggtgc ggtcgctcac ggtcaggacg accgcggagg
     2281 gcaccgcgcg ggcggacgtc cacgcctcct tcgccgggtt cgacgaggtc tccgtcgccg
     2341 cgctcgacgc cgcgggccgc cgtacgctgg tcatcgccag ccaccagttc gcgcgcccct
     2401 gccccgccga gcagccggtg ctgccggtca ccggtgccgc ccaggaccgg ccctggctcc
     2461 tggccaccgg gctgggcctg gtcgtcgtcg gcatcggcgt ccgcgcgcga accgcctacc
     2521 ggggcacaca cgcccgccga tgacccgacc ggccggaagg aggagcgtcc gatgctggaa
     2581 gagtgggacc tgtggtttcc aggcgccgca gccaccggga tctcgttcgc acgcagccgg
     2641 gtcgaggccg gagcggccgg cgaccggctg ctggtccacg ccgccccgcc caagctcgcg
     2701 gtcaccgttc gcgccgagga cggccaggta ctcgccgagg gtcacgacct cgagcgcggc
     2761 gagcccggcc ccatgagcta cctggtccgc cagggcgacg ccatccgact ggaggacggt
     2821 tggcccaccc aggacgacct gggacggctg gtgatcctgc ccggcgggga ggccggtgtc
     2881 ctcacaggct ggtggcacgc cgacgaccgc agcgagtggc gctggcagat cgagttgtac
     2941 aaccacgtct gaccctcgga ggcagccatg cgcgtcgccg tgctcgccga catccacggc
     3001 aacctgccgg cactggaggc ggtgctg
//
LOCUS       DASPYY010000453         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_778088, whole genome shotgun sequence.
ACCESSION   DASPYY010000453 DASPYY010000000
VERSION     DASPYY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778088"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="VEP73_07120"
     CDS             complement(<1..1320)
                     /locus_tag="VEP73_07120"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYT26236.1"
                     /translation="MASLVLGPLLRYVGEAQATVWVETDTPCEVEVLGHRSATFHVEG
                     HHYALVVVEGLEPGRAYAYQVRLDGGLAWPEPGSRFPPSSIRTLDPAGRGRPLKLVFG
                     SCRVAAPHEPPYTLPRAADKRGREVDALRTYALRMLAEPPEAWPHALFMLGDQVYADD
                     TSPGTREFIRSRRDTSQPPGEEVADFEEYTHLYLESWGEPVLRWLLSTVPSAMIFDDH
                     DVHDDWNTSAAWVASMRAEPWWQERIESALASYWLYQHLGNLAPAELAGDPLYAKVQA
                     AGDGGPVLRELAARADREAEGTRWSYHRDFGTTRLLVVDSRCGRVLDGSRREMLDPAE
                     WRWVEEGMTGEFDHLLVATSLPFLLAPGTHHLEAWNEAVCAGAWGRAMAKLGEQLRQA
                     ADLEHWAAFGSSFERLARLLLEVASGRRGPAPASVVLLSGDVHHAYLA"
     gene            complement(1376..1864)
                     /locus_tag="VEP73_07125"
     CDS             complement(1376..1864)
                     /locus_tag="VEP73_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26237.1"
                     /translation="MAWATSRSPGLFTPAAVALALLAAGLLAAGLALAWAPAVGWVLW
                     LCLAGYLLSLLGQPRRVDAGAPVVGAAMFLMAELAYWSIELRPPARQARGLAARRLAA
                     IGGVVLAAEALAALALVTASPGADLGLVLDAAGAAAAVAAVALLLGLLGRRLGGPDRP
                     PP"
     gene            complement(1897..3174)
                     /locus_tag="VEP73_07130"
     CDS             complement(1897..3174)
                     /locus_tag="VEP73_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYT26238.1"
                     /translation="MTRFPTHKLGAYTSVVALGLLGALASGLPELVAVVAPFAVLLVY
                     GLARSLVPRAEVRLALAEERVVEGEGVPLAVEVRAHTPLEQVEVLVPLPAGLALDGGP
                     NPTALRLAEGEAARLERAVRGTRWGLYEVGEVWLRVSDRFGLFQFESRSGRPAALRVY
                     PGARALRGLVRPRDTRVSAGNQVARPKGDGIEFADLRPFAPGDPARRVNWRASARQGA
                     LWVNEAHPERNTDIVLFLDTFAEARSADGSTLDSAVRAATALAARYLEGRDRVGLVRL
                     GGTLRWLVPGTGPTHLHRVVEALLEADASPTFVDKGIGLVPPRTLPPRALVIALTPLL
                     DGRGRAVLLDLHARGHDLAVVEVSPVAHVAPGGGELGELAMRLWRLEREALRARYERL
                     GIAVVAWRDDVELEAVLQEVAAFRRSARRVRAF"
     gene            complement(3171..4148)
                     /locus_tag="VEP73_07135"
     CDS             complement(3171..4148)
                     /locus_tag="VEP73_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT26239.1"
                     /translation="MPLDLGEATARARKALDEVERAVVGKRAALELVLCGILADGHVL
                     IEDVPGLAKTLIARSFAQVLSLDFARIQFTPDLMPSDVTGSSIYNQRTSDFEFRPGPV
                     FTNLVLGDEINRAPPKTQAALLEAMQERQVTIEGRTRHLGPPFLVLATQNPIEYEGTY
                     PLPEAQLDRFLLRVGVGYPAREDEWELLRRRLDRRSDDVALDPVVDRAALLAMQRAVE
                     EVFVDESIGWYIVDLVAATRASPRVRVGASPRGTLALLKLARVRALLDGRDFVTPEDV
                     KAVAGPALGHRLVLRPELWAQRVAGDDVVRECLAQTPAPAPAGPRPASR"
     gene            complement(4149..>4684)
                     /locus_tag="VEP73_07140"
     CDS             complement(4149..>4684)
                     /locus_tag="VEP73_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26240.1"
                     /translation="PRGRGGVTRRVVAWAALLAAAASGVAVLLALAPDRPVLGRVWLL
                     GLAVLAVLRLGQRARAGALRPRRSAYDRAARHGAGDPARPEDLRRLEQALSFATAREA
                     DLHGRLRPLLREVAELRLAARHGISLDGDPAAAAAALGPELWELLRPDRRPPARDAAP
                     GASPAALRAMVERLEAV"
BASE COUNT          606 a         1778 c         1832 g          468 t
ORIGIN      
        1 ggccaggtag gcgtggtgga cgtcgcccga cagcagcacg accgacgccg gcgccggccc
       61 gcgccggccg ctcgccacct cgaggagcag gcgtgcgagc cgctcgaacg agctgccgaa
      121 ggcggcccag tgctcgaggt cggcggcctg gcggagctgc tccccgagct tggccatggc
      181 gcgcccccag gcgcccgcgc agaccgcctc gttccacgcc tcgaggtgat gggtgcccgg
      241 ggcgagcagg aacggcagcg aggtcgccac gagcaggtgg tcgaactcgc cggtcatgcc
      301 ctcctcgacc cagcgccact ccgcggggtc gagcatctcc cgccgggacc cgtcgagcac
      361 ccgcccgcag cgggagtcga cgacgagcag cctggtcgtg ccgaagtcgc ggtggtagct
      421 ccagcgcgtc ccctcggcct cgcggtcggc cctggcggcg agctcgcgca ggaccggccc
      481 gccgtcgcct gccgcctgga ccttggcgta gagggggtcg ccggccagct cggccggtgc
      541 caggttgccg aggtgctggt agagccagta gctcgcgagc gcgctctcga tccgctcctg
      601 ccaccacggc tcggcgcgca tcgacgcgac ccaggccgcc gacgtgttcc agtcgtcgtg
      661 cacgtcgtgg tcgtcgaaga tcatcgcgct cgggacggtc gacagcagcc agcgcagcac
      721 cggctcgccc cacgactcga ggtagaggtg ggtgtactcc tcgaagtcgg cgacctcctc
      781 accgggcggc tggctggtgt cgcgccggga gcggatgaac tcccgcgtgc ccggcgaggt
      841 gtcgtcggcg tacacctggt caccgagcat gaagagcgcg tgcggccacg cctcgggcgg
      901 ctcggcgagc atgcggagcg cgtaggtgcg cagggcgtcg acctcgcggc cacgcttgtc
      961 ggccgccctc ggcagggtgt agggcggctc gtggggcgcg gcgacccggc aggagccgaa
     1021 gacgagcttc agcggccggc cgcggccggc cgggtcgagc gtgcggatgg agctcggcgg
     1081 gaagcggctg cccggctccg gccaggccag gccgccgtcc agccggacct ggtacgcgta
     1141 cgcgcggccc ggctcgagcc cctcgacgac caccagcgcg tagtggtgcc cttcgacgtg
     1201 gaaggtggcc gagcggtggc cgagcacctc cacctcgcag ggcgtgtcgg tctcgaccca
     1261 gacggtcgcc tgggcctccc cgacgtaacg gagcagcggt ccgaggacga ggcttgccat
     1321 gatgacaacc tatccaccag ggcgggccgc caaccgtccc cgggcacccg aacccctacg
     1381 gcggggggcg gtccgggccg ccgaggcggc ggccgagcag gccgaggagc agggcgaccg
     1441 cggcgacggc ggcggcggcg cccgcggcgt cgaggacgag gcccaggtcg gcccctgggg
     1501 acgcggtgac cagggcgagc gcggcgagcg cctcggcggc cagcaccacc ccaccgatcg
     1561 cggcgagccg ccgcgccgcc agcccgcgcg cctggcgggc cggcgggcgc agctcgatcg
     1621 accagtacgc gagctcagcc atgaggaaca tcgccgcgcc gaccaccggg gcgccggcgt
     1681 ccacccggcg cggctggccg agcagggaga gcaggtagcc ggcgaggcag agccacagca
     1741 cccagccgac cgcgggggcc cacgccaggg cgagcccggc ggcgagcagc ccggccgcga
     1801 gcagggcgag cgccaccgcg gcgggcgtga agagcccggg gctgcggctc gtcgcccagg
     1861 ccaccaccgc gcagcacgcg agcgcgccgg cggcggtcag aaggcgcgca cgcggcgcgc
     1921 ggaccgccgg aatgccgcca cctcctgaag gaccgcctcg agctcgacgt cgtcccgcca
     1981 cgccaccacc gcgatgccga gccgctcgta gcgggcgcgc agcgcctccc gctcgagccg
     2041 ccacaggcgc atggcgagct cgcccagctc gccgccgccc ggcgcgacgt gcgcgaccgg
     2101 ggagacctcg acgaccgcga ggtcgtggcc gcgggcgtgc aggtcgagca ggaccgcacg
     2161 gccccgcccg tcgagcagcg gcgtgagcgc gatcaccagc gccctgggcg ggagcgtgcg
     2221 cggcgggacg aggccgatcc ccttgtcgac gaaggtcggg gaggcgtccg cctcgagcag
     2281 cgcctccacg acgcggtgga ggtgggtcgg gccggtgccc ggcacgagcc acctgagcgt
     2341 cccgccgagc cggacgaggc cgacccggtc gcggccctcg aggtagcggg cggccagggc
     2401 ggtcgccgcc cggacggccg agtcgagcgt cgacccgtcg gccgagcggg cctccgcgaa
     2461 ggtgtccagg aacagcacga tgtcggtgtt gcgctccggg tgggcctcgt tgacccacag
     2521 cgccccctgg cgcgcgctcg cgcgccagtt gaccctgcgg gccgggtcgc cgggcgcgaa
     2581 cgggcgcagg tcggcgaact cgatcccgtc gcccttcggc cgggcgacct ggttgccggc
     2641 tgagacgcgg gtgtcgcgcg ggcggaccag cccccgcagg gcgcgcgcgc ccgggtacac
     2701 gcgcagcgcc gcgggccggc cgctgcggga ctcgaactgg aacaggccga agcggtcgct
     2761 gacccgcagc cacacctcgc cgacctcgta gagcccccag cgcgtccccc ggacggcgcg
     2821 ctcgaggcgg gcggcctcgc cctcggcgag gcgcagcgcg gtcgggttcg gcccgccgtc
     2881 cagggcgagg ccggcgggca gcggcaccag cacctccacc tgctcgagcg gggtgtgcgc
     2941 gcgcacctcg accgcgagcg gcaccccctc cccctcgacc acgcgctcct ccgccagcgc
     3001 caggcggacc tccgcgcgcg ggaccagcga ccgcgccagc ccgtagacga ggagcacggc
     3061 gaacggggcg acgacggcga cgagctcggg gagcccgctc gcgagcgcgc cgagcaggcc
     3121 gagcgcgacg accgaggtgt acgcgcccag cttgtgcgtg gggaacctgg tcatcggctg
     3181 gcggggcggg ggccggccgg ggccggcgcc ggcgtctgcg cgaggcactc gcggacgacg
     3241 tcgtcgcccg cgacccgctg cgcccacagc tccgggcgga gcaccagccg gtgcccgagc
     3301 gccgggcccg cgaccgcctt gacgtcctcc ggggtcacga agtcgcgccc gtcgagcagc
     3361 gcccggaccc gggcgagctt cagcagcgcg agcgtccccc gggggctcgc gcccacccgc
     3421 acccttgggc tcgcccgggt ggccgcgacc aggtcgacga tgtaccagcc gatgctctcg
     3481 tcgacgaaca cctcctcgac cgcgcgctgc atggcgagca gcgccgcccg gtcgacgacc
     3541 gggtcgaggg cgacgtcgtc gctgcgccgg tcgaggcggc gccgcaggag ttcccactcg
     3601 tcctccctgg ccgggtagcc gaccccgacc cggagcagga accggtcgag ctgcgcctcg
     3661 ggcagcgggt aggtgccctc gtactcgatc gggttctgcg tcgcgaggac caggaacggc
     3721 gggccgaggt ggcgggtccg gccctcgatc gtgacctgcc gctcctgcat cgcctcgagc
     3781 agggcggcct gcgtcttcgg cggcgcccgg ttgatctcgt cgccgaggac gaggttggtg
     3841 aacaccggac cgggccggaa ctcgaagtcg gaggtgcgct ggttgtagat cgacgagccg
     3901 gtcacgtccg acggcatgag gtcgggggtg aactggatcc tggcgaagtc gagcgagagc
     3961 acctgggcga acgagcgggc gatgagcgtc ttcgcgagcc cggggacgtc ctcgatcagc
     4021 acgtgcccgt cggccaggat gccgcagagc accagctcga gggcggcgcg cttgcccacc
     4081 acggcccgct cgacctcgtc gagcgccttg cgcgcgcggg cggtcgcctc gcccaggtcg
     4141 agcggcatct agaccgcctc gagccgctcg accatcgccc ggagcgcggc cgggctcgcg
     4201 ccaggggccg cgtcccgtgc gggcgggcgc cggtcgggcc gcagcagctc ccacagctcg
     4261 gggccgagcg cggcggcggc ggcggccggg tcgccgtcca gcgagatccc gtgccgggcc
     4321 gcgaggcgca gctcggcgac ctcccgcagc agcggccgca gccggccgtg caggtcggcc
     4381 tcgcgcgcgg tcgcgaagga gagcgcctgc tcgagccggc gcaggtcctc ggggcgggcc
     4441 gggtccccgg cgccgtggcg ggcggcccgg tcgtaggccg accggcgcgg cctgagggcg
     4501 ccggcacggg cccgctggcc gaggcgcagg accgccagca ccgcgaggcc gagcagccac
     4561 acgcggccga ggacgggccg gtcgggcgcg agcgcgagca gcacggccac gccggaggcg
     4621 gcggcggcga gcagcgcggc ccacgcgacg acccgccggg tcacgccgcc acgccctcga
     4681 ggtc
//
LOCUS       DASPYY010000454         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2183662, whole genome shotgun sequence.
ACCESSION   DASPYY010000454 DASPYY010000000
VERSION     DASPYY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183662"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VEP73_07145"
     CDS             <1..605
                     /locus_tag="VEP73_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HYT26241.1"
                     /translation="SGRRASGRRASEGAEEARAPRPGPPLQQVEHEASMGLFAAAAGV
                     RAPAVVLVGSFGNGAGLLVEHRVAGRSLERLPATAVDDALLAEAWRQVAVLHRAGIAH
                     CDLDPAAFVVDGRGLPWLVDYDRAQAAAGDRLLDRDVASLLAGLALAVDAGRVAAAAR
                     RALGPEAVDRAVALLGSRDLPAAVRRGLRAHPEVRERLAG"
     gene            780..>1986
                     /locus_tag="VEP73_07150"
     CDS             780..>1986
                     /locus_tag="VEP73_07150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT26242.1"
                     /translation="MAVKELQRPSGLPPEELPGLTARIRRDVAAAAGLEHPGATAVLD
                     LVEEAGRPFVVMELVDGTTVQELVGRHGALSVRRAAWIGLDLLAVLRAAHALGVVHGN
                     VHPGGVLVATDGTTKLTGLGTASLPRPPGSGPAGAVLAPERARGGPASAAADVWSVGA
                     VMHNAATGQAPDPAAGPGQAGRAGWLTPVLTRLLSAEAAARPTAAEAYALVEQAISEA
                     RRPEARPGVPNGTGQGVPDGTGQGVPNGTENEGHGPAGSTLAAATHGGPPEAAHGGPP
                     DGAGGPWMASGDPAPWGASPTNGHPLRPGGAGRRLRTGLVVGLVSLAVVALAFLGTVR
                     MLGRPAEKRAPSPTVANLAAGTVPTTGAVVAATASSPPTSTVPPTTVAPATAVPPTTV
                     APTTAPPATV"
BASE COUNT          192 a          804 c          772 g          218 t
ORIGIN      
        1 cctccgggcg gcgtgcctcc gggcggcgtg cctccgaagg ggccgaggag gcgcgggcac
       61 ccaggcccgg cccgcccctc cagcaggtcg agcacgaggc gtcgatgggc ctcttcgcgg
      121 cggctgccgg ggtgcgggcc ccggcggtcg tgctcgtcgg gtcgttcggc aacggcgccg
      181 gcctgctcgt cgagcaccgg gtggccgggc ggagcctcga acggctgccg gccacggccg
      241 tcgacgacgc cctcctcgcc gaggcctggc ggcaggtcgc cgtcctccac cgggcgggga
      301 tcgcccactg cgacctcgac ccggccgcct tcgtcgtcga cggtcgcggc ctgccctggc
      361 tggtcgacta cgaccgggcc caggcggccg ccggcgaccg gctgctcgac cgggacgtcg
      421 cgtcgctgct cgccggcctg gcgctcgccg tggacgccgg gcgggtcgcg gcggcggcgc
      481 ggcgggcgct cggcccggag gcggtcgacc gggcggtcgc gctgctcggg tcccgcgacc
      541 tcccggcggc ggtccggcgc ggcctgcgcg cccatcccga ggtacgcgag cggctcgccg
      601 gctgagcccg gcgcgcgctc agttggtggt agccggcgga ccgcggtagc ctcccgcgca
      661 tgccgccaga cgccggtccc cctcggcttc tcgccagccg ttactccctc gacgccgttc
      721 tcgacgagcg gggtgccggt gtggtgtggc gtgcccatga cgtcgcccgg gaccgtccgg
      781 tggccgtcaa ggagctccag cggccgtccg gcctgccacc ggaggagctg cccggcctca
      841 ccgcccgcat ccgccgggac gtggcggcgg ccgccgggct cgagcatccg ggcgcgaccg
      901 cagtgctcga cctggtcgag gaggccggga ggcccttcgt cgtgatggag ctggtcgacg
      961 gcacgaccgt gcaggagctc gtcggccggc acggcgccct ctcggtcagg cgcgcggcgt
     1021 ggatcggcct cgacctgctc gccgtgctcc gggccgccca cgcgctcggc gtcgtccacg
     1081 gcaacgtgca cccgggcggc gtgctggtcg cgacggacgg caccaccaag ctgaccgggc
     1141 tcggcacggc ctcgctgccc cgcccgccag gatccggccc ggcgggcgcg gtgctcgccc
     1201 ccgagcgggc gcgcggggga cctgccagcg ccgccgcgga cgtgtggtcg gtcggcgcgg
     1261 tgatgcacaa cgccgccacc gggcaggcac ccgatcccgc cgccggcccc ggccaggccg
     1321 ggcgtgccgg ctggctcacc ccggtgctca cccggctgct ctcggccgag gcggcggcgc
     1381 ggcccaccgc cgcggaagcg tacgccctgg tcgagcaggc catctcggag gcacgccgcc
     1441 cggaggcacg ccccggcgtg ccgaacggga ccggccaagg cgtgccggac gggacaggcc
     1501 aaggcgtgcc gaacgggaca gagaacgagg gccacgggcc cgcgggctcg acgctggccg
     1561 cagccacgca cggcgggccg ccggaggcgg cgcacggcgg gccgcccgac ggtgccggcg
     1621 gcccttggat ggcgtctggc gaccccgccc cgtggggggc ctctccgacg aacgggcatc
     1681 ccctacgccc gggcggcgcc gggcggcgcc tgcgcaccgg gctggtcgtt ggcctcgtct
     1741 cgctcgccgt ggtcgcgctc gcgttcctcg gcacggtccg gatgctcggc aggccggccg
     1801 agaagcgggc gccgtcgccg accgtggcca accttgcggc cggcaccgta ccgacgaccg
     1861 gggcggtcgt cgcggcgacc gcctcgtcgc cgcccacctc gaccgtcccg ccgaccaccg
     1921 tcgcccccgc caccgccgta ccgccgacga ccgtcgcccc gacgaccgcc ccgccggcaa
     1981 cggtcg
//
LOCUS       DASPYY010000455         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2604959, whole genome shotgun sequence.
ACCESSION   DASPYY010000455 DASPYY010000000
VERSION     DASPYY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604959"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="aceA"
                     /locus_tag="VEP73_07155"
     CDS             <1..1200
                     /gene="aceA"
                     /locus_tag="VEP73_07155"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HYT26243.1"
                     /translation="RVDASLARLGTERLWNLLNTDDFVPALGAMTGGQAVEMVKAGLK
                     AIYVSGWQTAADANLSEEVYPDQSLYPANSVPAVVRRVNNALQRADLVDRVEGRSGAS
                     WLVPIVADAEAGFGGALNAFELMLAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTA
                     QFIRTLTAARLAADVAGVPTVLVARTDALAATLLTSDVDERDRAFATGERTAEGYHVV
                     RGGLEAAVARALAYAPYADLLWCETSTPDLGQARRFAEAVHAEFPGKPLAYNCSPSFN
                     WRRHLDDAQIARFQKELGAMGYRFQFITLAGFHALNAAMFELAHGYAEEGMPAYVRLQ
                     EREFELEQAGYSATRHQREVGTGYFDAVAGVISAGTASTLALAGSTELEQFGTGGPDG
                     LPSSGSP"
     gene            1277..>1752
                     /locus_tag="VEP73_07160"
     CDS             1277..>1752
                     /locus_tag="VEP73_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26244.1"
                     /translation="MSDGGAMPGPGAGTAGRGTRLVLVVTVLAALLVQGAAAVALPRD
                     ASQLAQRAKEVQAQLDEQYNAIERLTEQFNETTDRQDGLRRQLAALERRRRQVQADLA
                     KAQKQLDDQVRATYMAGPGDVVGEFAGSPDLAEALNRVPLQRSVLEARAATLADVQ"
BASE COUNT          253 a          634 c          634 g          231 t
ORIGIN      
        1 cgcgtggacg cctcgctggc ccgcctgggg accgaacgcc tgtggaacct gctgaatacc
       61 gacgacttcg ttccggcgct cggagccatg acgggcggcc aggcggtgga gatggtgaag
      121 gcgggcctga aggccatcta cgtctccgga tggcagacgg ccgcggacgc gaacctatct
      181 gaggaggtgt acccggatca gagcctctac cccgccaaca gcgtccccgc ggtcgtccgg
      241 cgggtcaaca acgccctgca gcgcgccgac ctcgtcgacc gggtggaggg caggtccggc
      301 gcctcgtggc tggtgccgat cgtggccgac gccgaagccg gcttcggcgg cgcgctgaac
      361 gccttcgagc tgatgctcgc gatgatcgag gccggggcgg ccggagtcca tttcgaggac
      421 cagctcgcgt cggagaagaa gtgcgggcac ctgggcggca aggtgctggt gccgaccgcc
      481 cagttcatca ggaccctgac cgcggcgcgg ctggccgccg acgtggccgg cgtgcccacc
      541 gtcctggtgg cacggaccga cgccctggcc gcgacgctgc tcaccagcga cgtcgacgag
      601 cgcgaccggg cgttcgccac gggggagcgc accgccgagg ggtaccacgt ggtccgtggc
      661 ggcctcgagg cggcggtcgc acgcgcgctc gcctacgcgc cctatgccga cctgttgtgg
      721 tgcgagacgt cgacgcccga cctcgggcag gcccgtcgct tcgccgaagc cgtgcacgcc
      781 gagttccccg gcaagccgct cgcctacaac tgctcgccct cgttcaactg gcgcaggcac
      841 ctcgacgacg cgcagatcgc caggttccag aaggagctcg gcgccatggg ctaccggttc
      901 cagttcatca ccctggccgg gttccacgcc ctcaacgcgg ccatgttcga gctggcccat
      961 ggctatgccg aggaggggat gcccgcctac gtccggctcc aggagcggga gttcgagctc
     1021 gagcaggccg ggtactcggc caccaggcac cagcgggagg tcggcacggg ctacttcgac
     1081 gccgtcgcgg gcgtgatctc ggccgggacc gcctcgaccc tcgcgctggc cggctccacc
     1141 gagctggagc agttcggcac ggggggcccg gatggccttc cgtcgtcagg tagtccgtag
     1201 ttgtgctgag ttggaggtcc gcagaacccg gtactatgcc ctctggtccg gcgagtccgg
     1261 cgagaagggg gcaagcttgt ccgacggcgg cgccatgcca ggcccgggcg cgggcacggc
     1321 gggacggggc acgcggctcg tcctggtcgt cacggtgctc gcggccctgc tcgtccaggg
     1381 cgccgccgcc gtcgccctgc cccgcgacgc gagccagctc gcgcagcggg ccaaggaggt
     1441 ccaggcccag ctcgacgagc agtacaacgc catcgagcgc ctgaccgagc agttcaacga
     1501 gaccaccgac cgccaggacg ggctcaggag gcagctcgcc gcgctcgagc gccgccggcg
     1561 ccaggtccag gcggacctcg ccaaggccca gaagcagctc gacgaccagg tcagggcgac
     1621 ctacatggcc ggcccggggg acgtcgtcgg cgagttcgcc ggctcccccg acctggccga
     1681 ggcgctgaac cgggtcccgc tgcagcgctc ggtgctcgag gcccgcgccg ccaccctcgc
     1741 cgacgtgcag ca
//
LOCUS       DASPYY010000456         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_500525, whole genome shotgun sequence.
ACCESSION   DASPYY010000456 DASPYY010000000
VERSION     DASPYY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500525"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1770
                     /locus_tag="VEP73_07165"
     CDS             <1..>1770
                     /locus_tag="VEP73_07165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HYT26245.1"
                     /translation="AANSAVCYALGITKADAVSLHLLFERFLSPERDGPPDIDLDLEA
                     GRREEAIQYVYRRYGRDRVAQVANVITYRPRSAVRDMGRALGYAPGQLDAWSRQVEAW
                     GPLPAPGDHDVPPEVMELAAELQHFPRHLGIHSGGMVICDRPVVEVCPVEWATMADRS
                     VLQWDKDDCAAVGLVKFDLLGLGMLSMLHEAVDLVADHHGVDVDLAGLPQEPAVYDML
                     CQADSVGVFQVESRAQMATLPRLAPRRFYDLVVEVALIRPGPIQGGSVHPYIRRRNGD
                     EPVTYLHPLLERSLAKTLGVPLFQEQLMQMAVDVAGFTAAEADQLRQAMGSKRSTERM
                     ERLRARLYAGMAERGVTGETADRIYEKLAAFANFGFPESHSVSFAYLVYASAWVKLHY
                     PAAFLAALLNAQPMGFYAPNTLVADARRHGVTLLGPDVNASAATATLEPARERGGGGA
                     AHPAVRLGLSYVRTLGDDLAERIAEGRPYASMEELVRRAGAGQAQVEALATAGAFGCF
                     GLGRREALWAAGAVAQAGSDRLPGTVVGAHAPELPGMSPIELGAADLWATGLSGDSHP
                     VQFVRDRLDALGVVTAAGLAAVPP"
BASE COUNT          216 a          682 c          637 g          235 t
ORIGIN      
        1 ggcggccaac tcggccgtct gctacgcgct cggcatcacc aaggccgacg ccgtctcgct
       61 gcacctcctc ttcgagcgct tcctgtcgcc cgagcgggac ggcccgcccg acatcgacct
      121 cgacctcgag gcgggccgcc gtgaggaggc gatccagtac gtctaccggc gctacggccg
      181 cgaccgcgtc gcccaggtcg ccaacgtgat cacctaccgg ccgcgctcgg cggtgcgcga
      241 catgggcagg gcgctcggct acgccccggg ccagctcgac gcctggtcca ggcaggtgga
      301 ggcgtggggg ccgctcccgg cccccggcga ccacgacgtc ccgcccgagg tgatggagct
      361 ggccgccgag ctccagcact tcccccgcca cctcggcatc cactcgggcg ggatggtgat
      421 ctgcgaccgg ccggtggtcg aggtctgccc ggtcgagtgg gccaccatgg ccgaccgcag
      481 cgtcctgcag tgggacaagg acgactgcgc ggcggtcggg ctcgtcaagt tcgacctgct
      541 cgggctgggc atgctcagca tgctccacga ggcggtcgac ctcgtcgccg accaccacgg
      601 ggtcgacgtc gacctggccg ggctgcccca ggagccggcc gtgtacgaca tgctgtgcca
      661 ggccgactcg gtcggggtgt tccaggtcga gagccgggcg cagatggcca cgctgccccg
      721 cctggcgccg cggcgcttct acgacctggt ggtcgaggtc gccctgatcc gtcccggccc
      781 gatccagggc ggctccgtcc atccctacat ccgccgccgc aacggcgacg agcccgtcac
      841 ctacctccac ccgttgctgg agcggtccct cgcgaagacg ctcggggtgc cgctgttcca
      901 ggagcagctc atgcagatgg cggtcgacgt ggccgggttc accgccgccg aggccgacca
      961 gctccgccag gcgatgggct ccaagcgctc gaccgagcgg atggagcggc tccgggcccg
     1021 cctctacgcg ggaatggccg agcgcggcgt cacgggcgag accgccgacc ggatctacga
     1081 gaagctcgcc gccttcgcca acttcggctt tcccgagagc cactcggtgt cgttcgccta
     1141 cctggtctac gcgagcgcct gggtcaagct ccactacccg gccgccttcc tcgcggccct
     1201 gctgaacgcc cagccgatgg gcttctacgc acccaacacg ctggtcgccg acgcccgccg
     1261 ccacggcgtg accctgctcg gccccgacgt caacgcctcg gcggccaccg ccaccctcga
     1321 gcccgcccgg gagcgggggg gcggcggggc ggcgcacccg gccgtccggc tcggcctctc
     1381 ctacgtgcgc acgctcggcg acgacctcgc cgagcgcatc gcggagggcc gcccctacgc
     1441 gtcgatggag gagctcgtcc gccgggccgg ggcgggccag gcacaggtcg aggcgctggc
     1501 caccgcaggg gccttcgggt gcttcgggct cgggcggcgg gaggcgctct gggcggccgg
     1561 ggcggtggcc caggccggga gcgaccggct gcccgggacg gtggtcggcg cgcacgcgcc
     1621 cgagctgccc ggcatgagcc cgatcgagct gggggcggcc gacctctggg cgaccggtct
     1681 ttcaggggac agccacccgg tccagttcgt tcgcgaccgg ctcgacgccc tcggcgtggt
     1741 gacggcggcc gggctggccg ccgtcccgcc
//
LOCUS       DASPYY010000457         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2184790, whole genome shotgun sequence.
ACCESSION   DASPYY010000457 DASPYY010000000
VERSION     DASPYY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184790"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VEP73_07170"
     CDS             complement(<1..835)
                     /locus_tag="VEP73_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYT26246.1"
                     /translation="MAELPAGRIPLAWWRRRSLRARITILAALVVAGGLAAGAVLLVH
                     SLEASLLAALDSTARQRAQDVAALAASDNLPDPFPVAGGGTVLVQVVDAHGRVRAASA
                     GGDRAVPLLSPGQLAAARDRVPRFVAGSRLGEDAPLRVVAWPARLRGEDVTVIAAVSV
                     GNLADSVRVLRNALLLGVPALLTLLAALTWATVGSTLRPVGALRRGAEEITGTGASRR
                     LPVPVGHDEVHRLGVTLNRMLDRLEEAGGRQRAFVADAAHELRSPLAGMRAQLEVALE
                     HP"
     gene            complement(828..1505)
                     /locus_tag="VEP73_07175"
     CDS             complement(828..1505)
                     /locus_tag="VEP73_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26247.1"
                     /translation="MRVLVVEDEVRLAEALQRGLQAEGFAVDVAHDGEEGLHLATEQG
                     YDAVVLDVMLPRLNGYRVCQRLRGLGNWVPVLMLSAKDGEYDQAEGLDVGADDYLTKP
                     FSYVVLVARLRALLRRGAPRRPAVLEAGDLRLDPARREVRRGTTEVGLTPREFSLLEY
                     LMRRRDEVVPKQELLEHVWDAHYDGDPNVVEVYVGYLRRKIDAPFGRRSLETVRGAGY
                     RLVGTGG"
     gene            1701..>2282
                     /locus_tag="VEP73_07180"
     CDS             1701..>2282
                     /locus_tag="VEP73_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26248.1"
                     /translation="MQALSARPRLVRWAAPIGVVALLAGGIGLATRSEGAAPSLPPRT
                     AEQLLADVGRAEPPGMSGTVVETARLGLPALPTQGGGDLSVAGLVTGSHTARVWYAGH
                     DRFRLAVSGSLAETDVIRDGQDLWTYESAANRATHVLLPVDKAEKAPMATPAATPEQA
                     ARRVLDAVTPSTSVRVGRTSEVAGRAVYELVLAP"
BASE COUNT          279 a          888 c          865 g          250 t
ORIGIN      
        1 cgggatgctc gagggccacc tcgagctgcg cccgcatgcc tgccagcggg ctgcggagct
       61 cgtgggcggc gtccgcgacg aaggcgcgct ggcggccgcc cgcctcctcc agccggtcga
      121 gcatgcggtt cagggtgacc ccgagccggt ggacctcgtc gtggccgacc gggacgggca
      181 ggcgccgcga ggcgcccgtc cccgtgatct cctcggcgcc ccggcgcagc gcgccgaccg
      241 gccgcagcgt cgagccgacg gtggcccagg tgagcgcggc gagcagcgtg aggagcgcgg
      301 ggacgcccag cagcagggcg ttccgcagga cgcggacgct gtcggcgagg ttgccgaccg
      361 agacggcggc gatcacggtg acgtcctcgc cgcgcagccg cgccggccag gcgaccaccc
      421 ggagcggcgc gtcctccccc aggcgactgc ccgccacgaa gcggggcacc cggtcgcgcg
      481 ccgcggcgag ctggccgggc gacagcagcg ggacggcacg gtcgcccccg gccgacgcgg
      541 cgcggacgcg gccgtgcgcg tcgacgacct ggacgagcac ggtgccgccc ccggcgaccg
      601 ggaacgggtc gggcaggttg tcggacgccg ccagggccgc gacgtcctgt gcccgctgcc
      661 gcgcggtcga gtcgagggcc gcgaggagcg acgcctccag gctgtggacg agcaggacgg
      721 cgccggcggc gagcccgccc gcgaccacca gcgcagccag gatggtgatc cgggcgcgga
      781 ggctccgccg ccgccaccag gcgagcggga tgcgcccggc cggcagctca gccaccggtg
      841 ccgaccaggc ggtagccggc gccgcgcacc gtctcgagcg agcgccggcc gaacggcgcg
      901 tcgatcttgc ggcgcaggta gccgacgtag acctcgacga cgttggggtc gccgtcgtag
      961 tgggcgtccc agacgtgctc gaggagctcc tgcttcggca ccacctcgtc ccgccggcgc
     1021 atcaggtact cgagcaggga gaactcgcgt ggggtgagcc cgacctcggt cgtgccgcgg
     1081 cggacctccc gccgggccgg gtcgaggcgc aggtcgccgg cctcgagcac cgcggggcgc
     1141 cgcggcgcgc cgcggcgcag cagcgcgcgc agcctggcca cgagcacgac gtaggagaac
     1201 ggcttggtga ggtagtcgtc ggcgccgacg tcgaggccct cggcctggtc gtactcgccg
     1261 tccttggcgc tcagcatgag cacgggcacc cagttgccga gcccgcgcag gcgctggcag
     1321 acccggtagc cgttgagcct cggcagcatg acgtcgagca ccaccgcgtc gtagccctgc
     1381 tcggtcgcga ggtgcagtcc ctcctccccg tcgtgggcga cgtcgaccgc gaagccctcc
     1441 gcctgcagcc cccgctggag cgcctcggcc agccggacct cgtcctcgac gacgagcacg
     1501 cgcacgtccg cctcctctcc cctctccccg gccgggcgct ggtccagggt gcgccgacgc
     1561 tgctgagagc ctgctgagag ccggccgggc ctcctgagag cgtcctcagg cgcgctcagg
     1621 aacgccacag ctggcaacgc gcacactcgt cggtgtgctc agccggggcg acccggcgcc
     1681 ctccagaaag aaggtatggc atgcaggccc tctccgcccg cccgcgcctg gtgcgctggg
     1741 cggcacccat cggcgtcgtg gcgctgctcg ccggcggcat cgggctcgcg acgcgctccg
     1801 agggcgcggc gccgtcgctg ccgccccgca ccgccgagca gctcctcgcc gacgtcggcc
     1861 gggccgagcc gcccggcatg tccggcacgg tggtcgagac ggccaggctc ggcctgccgg
     1921 cgctgcccac gcagggcggc ggtgacctct cggtcgccgg cctcgtcacc gggtcccaca
     1981 ccgcccgggt ctggtacgcc ggccacgacc gcttccgcct cgccgtgtcc ggctccctgg
     2041 ccgagaccga cgtgatccgc gacggccagg acctgtggac ctacgagagc gcggccaacc
     2101 gggccacgca cgtcctcctc ccggtggaca aggccgagaa ggcgcccatg gcgaccccgg
     2161 cggcaacgcc cgagcaggcc gcgcggcggg tcctcgacgc cgtcacgccg tcgacctcgg
     2221 tgcgggtggg gcggacctcc gaggtggccg ggcgggcggt ctacgagctg gtcctcgcgc
     2281 cc
//
LOCUS       DASPYY010000458         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1905768, whole genome shotgun sequence.
ACCESSION   DASPYY010000458 DASPYY010000000
VERSION     DASPYY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905768"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VEP73_07185"
     CDS             <1..1489
                     /locus_tag="VEP73_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26249.1"
                     /translation="GRWVLLATILGSGVSFLDATVVNVALPTIGRQLGAGISTLQWVV
                     NAYTLTLAGFLLLGGSLGDHYGRRRVFVVGVSWFALASLLCGLAPNAQALVAARALQG
                     VGAALLMPGSLAIIEATFHPDDRGAAIGAWSGLGGVTTAVGPFLGGWLVEAVSWRLIF
                     FINLPLAAAVVWVATRHVPESSDPEAAPGIDYPGAALAALGLAGVIFALTEGPATGWT
                     SPAILLSGLGGLAALAAFVAVERRSRHPMLPLDVFRSRQFTAANLVTFVVYGALGGAL
                     FLVPIQLQRVLGYSPLASGVALLPITVVMLLLSARAGRLAQRIGPRLPMTVGPLAAAA
                     GLALLARVGPGATYAGDVLPAVVVFALGLSLTVAPLTSTVLAAASAEHAGVASAVNND
                     VARAAGLFAVAVLPAAAGLSGAGALEPAEFSAGFRMAVLLAAGLCALGGLLAFATIRD
                     VRRPGETAGPEARGPGLPGQAPEPCMSCPMTAPPPSRSAVGARRR"
     gene            complement(1508..2119)
                     /locus_tag="VEP73_07190"
     CDS             complement(1508..2119)
                     /locus_tag="VEP73_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HYT26250.1"
                     /translation="MIDALEPRDVLGLLAEEDRLRVAAAIVLGARGHAEIAAATGLPP
                     RAVVRAVARLTTGGLVEQAAGGGYRVRTEALAEAARRAASATAEPEPGADGLGVDDPQ
                     EAAVLRRFVDGGRLVSIPAGHAKRRVVLGWLARLFEPGRYYPESEVNEQLGAHHPDYA
                     TLRRHLVDEGFLQRRDQVYWRIGGAVDTAPPGSSDGTAPSGLS"
     gene            2266..>3101
                     /locus_tag="VEP73_07195"
     CDS             2266..>3101
                     /locus_tag="VEP73_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HYT26251.1"
                     /translation="MPKTVRTTYACTECGASTPRWVGRCPGCGAWNTLVEEVVERAGP
                     PRPAGPVTPAIPITDVAPQVDAYHPTGVGELDRVLGGGLVDGSVVLLAGEPGIGKSTL
                     LLGVLARLAAGGRVLYVCAEESREQVRLRAERLDALHPDLLLAAETDLGAIRTLIDDL
                     RPRVVVVDSVQTVADPELAGAPGGVGQVREVAAQLVRLAKELGIATFLVGQVTKEGAV
                     AGPKTLEHLVDVVLNFEGDEHHALRLVRCTKNRYGPAYEVGCFEMTEAGLVPLADPSR
                     LFL"
BASE COUNT          296 a         1231 c         1144 g          430 t
ORIGIN      
        1 cgggcgctgg gtgctgctcg cgacgatcct cggctccggc gtctcgttcc tcgacgccac
       61 ggtcgtcaac gtggccctgc cgacgatcgg ccgccagctc ggcgccggca tctccaccct
      121 gcagtgggtg gtcaacgcct acacgctcac cctcgccggc ttcctgctgc tcggcggctc
      181 gctcggcgac cactacggcc gcaggcgggt gttcgtcgtc ggggtgtcct ggttcgcgct
      241 cgcgtccctg ctctgcgggc tcgcgccgaa cgcgcaggcg ctggtcgccg cccgggcgct
      301 ccagggcgtc ggcgcggcgc tgctcatgcc ggggagcctc gcgatcatcg aggcgacctt
      361 ccaccccgac gaccggggcg ccgcgatcgg cgcctggtcc ggtcttggcg gcgtgacgac
      421 ggcggtcggg ccgttcctcg gcggctggct cgtcgaggcg gtgagctggc ggctcatctt
      481 cttcatcaac ctgcccctgg ccgcggcggt cgtctgggtg gcgacgaggc acgtgcccga
      541 gtccagcgac cccgaggcgg cccccgggat cgactacccc ggtgccgcgc tggccgcgct
      601 cggcctggcc ggggtcatct tcgcgctcac cgaggggccg gcgacgggct ggacgtcccc
      661 ggcgatcctg ctctccgggc tcggcggcct ggccgcgctg gccgcgttcg tcgcggtcga
      721 acggcgcagc cgccacccga tgctgccgct cgacgtgttc cggtcgcgcc agttcacagc
      781 ggccaacctg gtcaccttcg tggtctacgg cgccctcggc ggcgcgttgt tcctggtccc
      841 gatccagctg cagcgcgtgc tcggctactc gccgctcgcg tccggcgtcg ccctgctccc
      901 gatcaccgtg gtgatgctgc tgctctcggc ccgcgccggc cggctcgccc agcggatcgg
      961 gccgcgcctc ccgatgacgg tcgggccgct cgcggccgcc gccgggctcg cgctgctcgc
     1021 gcgggtcggc cccggcgcga cctacgccgg ggacgtcctg cccgcggtgg tcgtgttcgc
     1081 gctcggcctg tcgctgaccg tcgccccgct cacctcgacc gtgctcgccg cggccagcgc
     1141 cgagcacgcc ggcgtcgcga gcgcggtcaa caacgacgtg gccagggcgg ccggcctgtt
     1201 cgcggtggcc gtgctgcccg ccgcggccgg gctctccggc gccggcgcgc tcgagcccgc
     1261 cgagttcagc gccgggttcc ggatggccgt cctgctcgcg gccggcctct gcgccctggg
     1321 cgggctgctc gcgttcgcga cgatccgcga cgtgcgccgc cctggcgaga cggccggacc
     1381 cgaggcccgc gggccgggcc tgcccgggca ggcgccggag ccgtgcatga gctgccccat
     1441 gaccgccccg ccgccgagcc ggtcggccgt cggcgcccgg cggcggtgac gaccggcgcc
     1501 gaggcggcta cgagagcccc gagggcgcgg tgccgtcgga ggacccgggc ggcgccgtgt
     1561 cgaccgcgcc gccgatgcgc cagtagacct ggtcgcgccg ctgcaggaac ccctcgtcca
     1621 ccaggtggcg ccgcagggtg gcgtagtcgg ggtggtgcgc cccgagctgc tcgttgacct
     1681 cggactccgg gtagtaccgg cccggctcga acagccgggc cagccagccg agcacgaccc
     1741 gccgcttggc gtggccggcc gggatcgaga cgagccggcc gccgtccacg aaacggcgca
     1801 gcacggccgc ctcctgcggg tcgtcgacgc cgaggccgtc cgcgcccggc tccggctcgg
     1861 ccgtggcgct cgccgcgcgc ctggccgcct cggcgagcgc ttcggtccgc acgcggtacc
     1921 ccccgcccgc cgcctgctcg acgaggccgc ccgtggtcag gcgggcgacc gcccggacga
     1981 cggcccgcgg tggcagaccg gtggcggcgg cgatctcggc gtggccgcgg gcgccgagga
     2041 cgatcgcggc cgcgacgcgc agccggtcct cctcggcgag caggcccagg acgtcccttg
     2101 gctccagtgc gtcgatcatg gcggctccgc cggattcttg gagtggcgtg ctagcctctt
     2161 tctatcaccg ggctgtgcgg cccgtattcc catcgcatcg cacgcccgcg cggggcagcg
     2221 gccgggaccc ggcccccggc ccccccggga tgggagcctt ctccagtgcc gaagaccgtc
     2281 cgtacgacct atgcgtgcac cgagtgcggg gcgtcgaccc cgcgctgggt cggccgctgc
     2341 cccggctgtg gcgcctggaa caccctcgtc gaggaggtgg tcgagcgcgc cggcccgccc
     2401 cgccccgccg gcccggtcac cccggccatc cccatcaccg acgtcgctcc ccaggtcgac
     2461 gcctaccacc cgaccggggt gggggagctc gaccgcgtgc tgggcggggg cctggtcgac
     2521 ggcagcgtcg tgctgctcgc gggcgagccc gggatcggca agtcgaccct gctgctcggc
     2581 gtcctcgccc gcctggcggc cggggggcgg gtcctctacg tctgcgccga ggagtcccgc
     2641 gagcaggtcc gcctgcgggc cgagcggctc gacgccctcc atcccgacct gctcctcgcc
     2701 gccgagaccg acctcggcgc catccggacg ctgatcgacg acctgcggcc ccgggtcgtg
     2761 gtggtcgact ccgtccagac ggtcgccgac ccggagctgg ccggcgcgcc cggcggcgtc
     2821 ggccaggtcc gcgaggtcgc ggcgcagctc gtccggctcg ccaaggagct cggcatcgcc
     2881 accttcctgg tcggccaggt caccaaggag ggcgcggtgg ccgggcccaa gaccctcgag
     2941 cacctcgtcg acgtggtgct gaacttcgag ggcgacgagc accatgcgct gcgcctggtg
     3001 cgctgcacca agaaccgcta cgggccggcg tacgaggtcg gctgcttcga gatgaccgag
     3061 gccgggctcg tgccgctcgc cgacccgtcc cgcctgttcc t
//
LOCUS       DASPYY010000459         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1905798, whole genome shotgun sequence.
ACCESSION   DASPYY010000459 DASPYY010000000
VERSION     DASPYY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905798"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEP73_07200"
     CDS             <1..227
                     /locus_tag="VEP73_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HYT26252.1"
                     /translation="AAALDGFTGTDTSSCVERYGGLRVQVVPGDPRNLKVTYADDLFT
                     AERLLAASGAGAGRSGACEASGMATRVRGR"
     gene            238..1605
                     /locus_tag="VEP73_07205"
     CDS             238..1605
                     /locus_tag="VEP73_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HYT26253.1"
                     /translation="MEDVLRTPLPSSDERYAAGRALRERMPRGALGRWRPSEDRPDPV
                     DLVEEAHAGRLERLVPLRVGRMVASPYAFLRGAASLMAEDFAGLPRTGIEPVICGDAH
                     LGNFGFYASPERDLVFDLNDFDEAHPGPWEWDLWRLATSVWVAGRQNGLAEGTCEDAA
                     LWCAAAYRRQVRRLAGQPLLARSFDRLDIDRMRQDLADWSLRGEIERAANLARGRTSD
                     RALPKLTRERGGVRHIVDQPPVVMHVSEEETGRLVDGLEAYLDTLRPHWRRVLASYVV
                     VDLAHKVVGVGSVGLRAFVALLQGNGQDDVVFLQLKQARRSCVARFVHGDSAWHAHQG
                     QRVVEYQQTLQTVSDPLLGWTAVDGRDYYVRQFRDMKGAVTVDGMDGSALMDYARVCG
                     TLLAKGHARSTGATMLSGYLGKGDSADRAFARFARAYADQTEADHLALRQAVKSGRLP
                     AEHGA"
     gene            complement(1935..2573)
                     /locus_tag="VEP73_07210"
     CDS             complement(1935..2573)
                     /locus_tag="VEP73_07210"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HYT26254.1"
                     /translation="MTVGEDGVARCWWCGDDRLYRSYHDVEWGRPSSDDRRLFEKLCL
                     EGFQAGLSWLTILRKREHFRRAFEDFDVEAVARFGAADVDLLLADAGIVRNRAKIQAT
                     IGNARRCAELVDEFGSLAAYVWRFEPDARSRPPVVDQATLMQLAQSPESKAMSNDLRR
                     RGWSFVGPTTVYSFMEAMGLVNDHLDRCDVRADVEHERRAFPRPPSAGQQRA"
     gene            2769..>2972
                     /locus_tag="VEP73_07215"
     CDS             2769..>2972
                     /locus_tag="VEP73_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26255.1"
                     /translation="MAAEPGRLPPCRRRGGRPRRLAGIFTIVTLLAGCHPATPDNQAL
                     LAALRDGRRAEVTVQGRVVDLLPD"
BASE COUNT          382 a         1101 c         1110 g          379 t
ORIGIN      
        1 aggccgccgc cctggacggg ttcacgggaa ccgacacgtc ctcctgcgtc gagcgctacg
       61 gcggtctccg cgtgcaggtc gtgccgggcg acccgcgcaa tctcaaggtg acctacgccg
      121 acgacctgtt cacggccgag cgactgctcg ccgcctcggg ggcaggggct gggaggtcag
      181 gggcctgcga ggcgagcggc atggcgaccc gcgtgcgcgg gcggtaggag gttcccggtg
      241 gaggacgtgc tgcggacccc gctgccctcc agcgacgagc gctacgcggc ggggcgggcg
      301 ctgcgcgagc ggatgccgcg cggtgcgctt ggccgctggc ggccctcgga ggaccggccc
      361 gacccggtgg acctggtcga ggaggcgcac gccggccggc tggaacggct ggtccccctg
      421 cgggtcggcc ggatggtggc gtcgccctac gccttcctgc gcggcgcggc ctccctgatg
      481 gcggaggact tcgcggggct gccgcggacc ggcatcgagc cggtcatctg cggcgacgcc
      541 cacctgggca acttcgggtt ctacgcctcg ccggagcggg atctcgtgtt cgacctcaac
      601 gacttcgacg aggcccatcc cggcccgtgg gagtgggacc tgtggcggct cgccaccagc
      661 gtgtgggtgg ccggccggca gaacggcctg gccgagggca cctgcgagga cgccgcgctg
      721 tggtgcgcgg cggcctaccg gcggcaggtc cggcggctcg ccgggcagcc gctgctggcc
      781 cgctcgttcg accggctgga catcgaccgg atgcggcagg acctggccga ctggtcgctg
      841 cgaggggaga tcgaacgggc ggcgaacctg gcccgtggcc gcaccagcga ccgggcgctg
      901 cccaagctga cccgcgagcg cggcggcgtg cgccacatcg tcgaccagcc gccggtggtg
      961 atgcacgtgt ccgaggagga gaccgggcgg ctggtcgacg ggctcgaggc gtacctggac
     1021 acgctgcggc cccactggcg ccgggtgctc gcctcgtacg tggtcgtcga cctggcccac
     1081 aaggtggtcg gcgtgggcag cgtcggcctg cgcgcgttcg tcgccctgct ccagggcaac
     1141 ggccaggacg acgtggtctt cctgcagctc aagcaggcca ggcggtcgtg cgtggcccgc
     1201 ttcgtgcacg gcgacagcgc ctggcacgcc caccaggggc agcgggtggt ggagtaccag
     1261 cagacgctgc agaccgtcag cgacccgctg ctcggctgga ccgcggtgga cggccgcgac
     1321 tactacgtcc gccagttccg cgacatgaag ggtgcggtca ccgtcgacgg catggacggc
     1381 tctgcgctca tggactacgc ccgggtctgc ggcacgctgc tcgccaaggg ccacgcccgc
     1441 tcgaccggcg ccacgatgct gtccggctac ctcggcaagg gcgacagcgc cgaccgggcg
     1501 ttcgcgcgct tcgcccgtgc ctacgccgac cagaccgaag ccgaccacct ggcgctccgg
     1561 caggctgtca agtccggccg gctcccggcc gagcacgggg cgtagcggga gcgggcgtcg
     1621 gccgggccgt cagcgggcca ccgtgctgcc ccggacgctg tcggacccgg ccagccgctg
     1681 cgcgaggtag acggggacga cggacagcag gacggcgagc gcgccgaagt aggcgaccac
     1741 cagccagccg accgggccgg cgagcagcag gccgagctgg agccggcggc ggccgcccga
     1801 tgaagggctg gatcgcggtc gacccgcgca gcctcgaggc cgacgacgcc ctggccgcct
     1861 gggtggacga tgcgctcagc ttcgtgcgga cgcttccgcc gaagtagcga cccccgctcg
     1921 accgggccgc gcgctcatgc ccgctgctgc cccgccgacg gcggtcgcgg gaacgcgcgg
     1981 cgctcgtgct cgacgtcggc gcgcacgtcg caccggtcga gatggtcgtt cacgaggccc
     2041 atcgcctcca tgaacgagta cacggtcgtg gggcccacga acgaccaccc ccggcgccgc
     2101 aggtcgttgc tcatcgcctt cgattccggg ctctgcgcga gctgcatcag ggtggcctgg
     2161 tcgacgacgg ggggacgcga gcgcgcgtcg ggctcgaacc gccacacgta cgcggcgagg
     2221 ctgccgaact cgtcgaccag ctcggcgcag cggcgcgcgt tgccgatcgt cgcctggatc
     2281 ttcgcccggt tgcggacgat ccccgcgtcg gccaggagca ggtcgacgtc cgccgcgccg
     2341 aaccgggcga cggcctcgac gtcgaagtcc tcgaacgccc gccggaagtg ctccctcttg
     2401 cggaggatcg tgagccagct cagccccgcc tggaacccct ccaggcagag cttctcgaac
     2461 aggcggcggt cgtcgctgct ggggcggccc cactccacgt cgtggtagct gcggtacagc
     2521 cgatcgtcgc cgcaccacca gcaccgggcg acgccgtcct ccccgacggt gataccggcg
     2581 gtcccaggcg acgccgcctc ggccccggcg tccgcccggc ctcgaacgac ctggtcgaag
     2641 tcgcccacgg cacctcctct ctcgcgcgca cctgccttgc ggggggctcg gagacctcgg
     2701 cgggcctggc ccggtgtgcg ctcctggggc gagaatgacc ctacttcatc gagccggagc
     2761 atgaccgtat ggcagctgaa cccggccgcc tgcccccctg ccgccgacgg ggaggccggc
     2821 cgcggcggct cgccggcatc ttcaccatcg tgacgctgct ggccggctgc cacccggcca
     2881 cgcccgacaa ccaggcactg ctcgccgccc tgcgggacgg gcgccgggcg gaggtcaccg
     2941 tccagggccg ggtcgtcgac ctgctgcccg ac
//
LOCUS       DASPYY010000460         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_641477, whole genome shotgun sequence.
ACCESSION   DASPYY010000460 DASPYY010000000
VERSION     DASPYY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641477"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..552)
                     /locus_tag="VEP73_07220"
     CDS             complement(10..552)
                     /locus_tag="VEP73_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYT26256.1"
                     /translation="MTEPERLSLAEWLVLCLVREEPTYGRVLVGLLARDGRLGQVWSV
                     PKAVVYRALQRLETLGLIRTVGEQRTSLGPVRSLFAATLAGQAAAVAWLSTPVEHARD
                     VRSELMVKLALLDRSGIDSRDLLQAQLARLLPVAAALDDRLRATTGFEHTLVLWRHEA
                     MTATLRFLETLTAQSEIRTS"
     gene            complement(563..1420)
                     /locus_tag="VEP73_07225"
     CDS             complement(563..1420)
                     /locus_tag="VEP73_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT26257.1"
                     /translation="MIPAKFDYVRPGSLDEAVSALAGGGDDAKVIAGGQSLLPLLRLR
                     LAYPELLVDVGGLDELRGVTDAGDALVIGARTTLYDLVRDPLVAEHAGIFAQTAGVIA
                     DPAVRHRATLGGALAHADPAGDLPAVALAFDAALVVRGPGGERDIPATDFFVDYLTTS
                     LAPDEILTAVRVPKLGPGWGYRYEKFHRTAQAWATVGVAALVRRSNGSVAEARIGLTN
                     MGTTPLRAAAAESAAAGAEASRDALRAAAASADEGTNPPADLHGAADYRRHLARVLTG
                     RALAAAAGA"
BASE COUNT          186 a          575 c          551 g          194 t
ORIGIN      
        1 aacttcgggc tagctggtcc ggatctcgct ctgcgcggtc agcgtctcca ggaaccgcag
       61 ggtcgcggtc atcgcctcgt gccgccacag gacgagggtg tgctcgaacc cggtggtggc
      121 ccgcaggcgg tcgtcgaggg cggcggcgac gggaagcagc cgggccagct gtgcctggag
      181 caggtcccgg gagtcgatcc cgctccggtc gagcagcgcg agcttgacca tgagctcgga
      241 ccgcacgtcc cgggcgtgct ccaccggcgt gctgagccac gccacggcgg ccgcctgccc
      301 ggccagcgtc gcagcgaaga gcgagcggac cggccccagg ctggtccgct gctctcccac
      361 cgtccggatc aggccgaggg tctcgagccg ctgcagtgcg cggtagacca cggccttcgg
      421 caccgaccag acctggccca ggcgcccgtc gcgggccagc agcccgacga ggacccggcc
      481 gtaggtcggc tcctcgcgga ccaggcacag caccagccac tcggccagcg acagccgctc
      541 cggctcagtc atggttccac ggtcaggctc ccgccgccgc ggccagcgcc cgcccggtga
      601 gcacccgggc caggtgccgc cggtagtcgg ctgctccgtg caggtcagcg ggcgggttcg
      661 tgccctcgtc cgcgctggcc gccgcggccc gcagcgcgtc gcggctggct tcggccccgg
      721 ccgcggcgct ctccgcggcc gccgctcgca gcggcgtcgt gcccatgttg gtgagcccga
      781 tcctggcctc ggcgacggac ccgttggacc gccggaccag cgcggccacg ccgaccgtcg
      841 cccaggcctg cgcggtccgg tggaacttct cgtaccggta accccagccg ggtccgagct
      901 tgggcacccg gaccgcggtc aggatttcgt ccggcgccag gctcgtggtc aggtagtcga
      961 cgaagaagtc cgtcgcgggg atatcgcgct cgccgccggg cccccggacg accagggcgg
     1021 catcgaacgc cagggcgacg gcgggcaggt cgccggccgg atcggcgtgc gcgagcgcgc
     1081 cgcccagcgt ggctcggtgc cggacggcgg ggtcggcgat cacgccggcg gtctgcgcga
     1141 aaatcccggc gtgctcggcc acgagcggat cgcggacgag atcgtagagt gtagtccgcg
     1201 ccccgatgac cagggcgtcc ccggcgtcgg tgacgccgcg cagctcgtcc aggccgccca
     1261 cgtcgaccag cagctccgga taggccagcc gcagccgcag caacgggagc aggctctgcc
     1321 cgcccgcgat gaccttggcg tcgtcgccgc ccccggccag ggctgagacc gcctcgtcca
     1381 gcgagccggg acggacgtaa tcgaacttcg ccgggatcat gctctgtcct ccgctcgctt
     1441 cgggggcgcc tcaaggcgcc gccttgcttg cctggccgcc tgcggcggcg ggccctcgtc
     1501 gctcct
//
LOCUS       DASPYY010000461         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2606099, whole genome shotgun sequence.
ACCESSION   DASPYY010000461 DASPYY010000000
VERSION     DASPYY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606099"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEP73_07230"
     CDS             complement(<1..413)
                     /locus_tag="VEP73_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HYT26258.1"
                     /translation="MATRARPSGSGLPRLVVAGTHSGVGKTTVATGLMAAFAARGHTV
                     AGFKVGPDFIDPSYHALATGRPGRNLDAFLSGPELIGPLFAHGAAGADLAIVEGVMGL
                     FDGAAGAGELASTAQVAKLLGAPVLLVLDCRALARS"
     gene            complement(421..1005)
                     /gene="cobO"
                     /locus_tag="VEP73_07235"
     CDS             complement(421..1005)
                     /gene="cobO"
                     /locus_tag="VEP73_07235"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYT26259.1"
                     /translation="MDSLNVTEPAQAAFDRARRAKRDHPLLIVNTGHGKGKSTAAFGI
                     MLRGWARGYRVGVFQFVKSGKWRIGERKAAEALGGITWESMGDGWTWVSRDLDQSADL
                     AREGWEEVKRRLAAETYELLILDELTYAMTFGWIDTAEVVATLRDRPGFQHVVVTGRD
                     APAELVDAADLVTEMRKLKHPYEQGIRGQQGIEW"
     gene            complement(1009..1902)
                     /locus_tag="VEP73_07240"
     CDS             complement(1009..1902)
                     /locus_tag="VEP73_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT26260.1"
                     /translation="MAAPGNVFVPVWIALEGRGAGAWGRRSRAEASGGHPVADRQGQG
                     HGHERSGLADLALAATLRAAALRLDRDRDQNDCDEATPGPAFRREDLRWWVRAGREGN
                     LILFVLDSSGSMAARRRMTATKGAVLSLLLDAYQRRDRIGLVTFGGAGATVALPPTSG
                     VELAAQRLAALPTGGRTPLAAGLARARQVLAAEAVRDPQRRPLLVVVTDGRANAAGGV
                     GAALAEARALASLGVGAVVVDTEDAAVRLGLAARVAAALGAPCVRLEQLAAQPLAGIV
                     RVLAGSASGTGRRPGRSRKVA"
BASE COUNT          274 a          828 c          830 g          301 t
ORIGIN      
        1 gagcgagcca gcgcccggca gtcgagcacc agcagcaccg gcgcgccgag cagcttcgcg
       61 acctgtgcgg tggaggcgag ttcgcccgcg cccgccgcgc cgtcgaacag ccccatgacc
      121 ccttcgacga tggccagatc ggccccggcg gcgccgtggg cgaagagcgg cccgatcagc
      181 tccgggccag acaggaaggc gtcgaggttg cggccgggcc gtccggtggc gagggcgtgg
      241 taggacgggt cgatgaagtc cggcccgacc ttgaagcccg cgacggtgtg gccgcgcgcg
      301 gcgaacgccg ccatcagccc ggtcgccacg gtggtcttgc cgacgccgga gtgggtgccg
      361 gccacgacca gccgggggag ccccgacccg ctcggcctcg ccctcgtcgc catcacgcct
      421 tcaccactcg atcccctgct ggccgcggat gccctgctcg taggggtgct taagcttgcg
      481 catctcggtg accaggtcgg cggcgtcgac cagctcggcg ggcgcgtcac ggccggtgac
      541 caccacatgc tggaagccgg gtcggtcccg cagggtggcg accacctcag cggtgtcgat
      601 ccagccgaag gtcatcgcgt acgtcagctc gtcgaggatc agcagctcat aggtctcggc
      661 ggcaaggcgg cgctttactt cttcccagcc ctcgcgggcg aggtcggcgg actggtcgag
      721 gtcgcgtgac acccaggtcc agccgtcgcc catgctctcc caggtgatgc cgccgagcgc
      781 ctcggccgct ttgcgctcgc cgatgcgcca cttgccggac ttgacgaact ggaagacccc
      841 gacccggtag ccgcgcgccc agcctcgcag catgatcccg aacgccgcgg tggacttccc
      901 cttgccgtgg ccggtgttga cgatcagcag cgggtggtcg cgcttggccc gccgggcccg
      961 gtcgaacgcc gcctgcgctg gttcggtcac gttcaaggag tccataactc acgccacctt
     1021 tctcgaacga cccggccggc ggcccgtgcc gctggcgctg ccggcgagga cccggacgat
     1081 gccggcgagc ggctgggccg cgagctgctc cagccgtacg cacggcgcgc cgagtgcggc
     1141 ggcgacgcgg gcggcgagcc cgagccggac ggcggcgtct tcggtgtcga cgacgacggc
     1201 gccgacgccg aggctggcaa gcgcccgcgc ctcggcgagc gccgcgccga caccaccggc
     1261 ggcgttggcg cgtccgtcgg tgacgaccac caggagcggc cgccgctgcg ggtcgcggac
     1321 ggcttcggcg gcgagcactt ggcgggcacg ggcgagccca gccgcgagcg gggtacgccc
     1381 tccggtcggc aacgccgcca gccgctgggc ggcgagttcc accccggagg tcggcggcag
     1441 cgccacggtg gcgccagcgc cgccgaaggt caccagcccg atccggtccc ggcgctggta
     1501 ggcgtcgagc agcagcgaca gcaccgcgcc cttggtcgcg gtcatgcgcc tgcgggcggc
     1561 catcgagccg ctgctgtcga gcacgaacag gatcaggttg ccctcccggc ccgcccgcac
     1621 ccaccagcgc aggtcctcgc gccggaaggc cggccctggc gttgcctcgt cacagtcatt
     1681 ctggtcccgg tcccggtcga ggcgcagtgc cgccgcgcgg agggtcgcgg ccagcgccag
     1741 gtcggcaagg ccgctgcgct cgtggccatg gccctggccc tggcggtcgg caaccgggtg
     1801 gccgccgctc gcctcggcgc ggctgcgccg cccccacgcg ccggcgccac ggccctccag
     1861 ggcgatccac accggcacga agacgttccc gggtgctgcc acctggagct gtccggccct
     1921 tttctctgct ccagaggctg tggataaccc ctgcgcaccc ccagcggttt ccgatagcgt
     1981 tgaaacaggg ggacccccct cggctggggg ggtatttggt gtgcccggtg cgcctgaccc
     2041 gccgggtgtg cctgggctgg acgtcgggtc gagcgcggcg gggtccgcag tgccgttgcc
     2101 gtggcggctg tcatgggggc ctacggggct gcggcggcca gctccatttc ctgcgccgtg
     2161 cccggcggaa ctgtcggagc cgtcggagcg gctggagcgg tcgggcccgg cgggatccgg
     2221 gtcgtcgtcc ggg
//
LOCUS       DASPYY010000462         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1061891, whole genome shotgun sequence.
ACCESSION   DASPYY010000462 DASPYY010000000
VERSION     DASPYY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061891"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEP73_07245"
     CDS             <1..510
                     /locus_tag="VEP73_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26261.1"
                     /translation="WGLARGYDALVEMDADLSHPPERLPALLDGLAEADLTIGSRYVP
                     GGRTVNWSRLREAISRGGNAYVRIALGVPIHDVTAGYRAYRREVLEALPVAAVQSNGY
                     CFQVEMAHRTWREGFRVVELPITFTERVSGVSKMSRQIVAEALLRVGIWALTGGRRRH
                     RDRHPASVA"
     gene            562..978
                     /locus_tag="VEP73_07250"
     CDS             562..978
                     /locus_tag="VEP73_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26262.1"
                     /translation="MWGRRAADPAVEAAWERFGALAGALDEGSRALLATVPSARRPGA
                     PLDACVAGFLAGIAAARDTLPGWALPELAGAQAATAAALDAAERRARGLAAATEGLEF
                     EHRNAAVAEVLDELAPVQDAEVALRRLRRRAARSRR"
     gene            1108..>1747
                     /locus_tag="VEP73_07255"
     CDS             1108..>1747
                     /locus_tag="VEP73_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT26263.1"
                     /translation="MTFATPVLLLGLLLVPLVLLALVLAQRRGVRYAVRYPALDVLAG
                     VVGRTPGWRRHLPAALLLLALTALLLGTARPMARVPVPRDQATVLLVVDVSGSMNADD
                     VDPTRLGAARAAAGRFLDRLPERFQVGLVAFASGAQTLVRPTTDRAAVRQALETLQAG
                     GGTAMGDGLARALDSVEAARRAAGGGAKRATPGRSGQAASPPASRPAVALLLS"
BASE COUNT          176 a          682 c          677 g          212 t
ORIGIN      
        1 tgggggctcg cgcgcggcta cgacgccctc gtcgagatgg acgccgacct gtcccatccc
       61 cccgagcggc tccccgcgct gctcgacggg ctcgccgagg ccgacctcac gatcgggtcg
      121 cgctacgtcc ccgggggccg cacggtcaac tggtcgaggc tccgcgaggc gatctcccgc
      181 ggcggcaacg cctacgtgcg gatcgccctc ggcgtgccga tccacgacgt caccgccggc
      241 taccgcgcct accggcgcga ggtgctcgag gcgctgccgg tggcggcggt ccagtccaac
      301 ggctactgct tccaggtcga gatggcccac cgcacctggc gggagggctt ccgggtcgtc
      361 gagctgccga tcaccttcac cgagcgggtg agcggcgtct ccaagatgag caggcagatc
      421 gttgccgagg cgctcttgcg ggtggggatc tgggcgctga ccggcggccg ccggcgccac
      481 cgcgaccgcc accccgcgag cgtcgcgtag gcagaggaac cgcaggccag gggggcgcga
      541 ccagcccgac aggaggatgc catgtggggc cggcgggccg ccgacccggc ggtggaggcg
      601 gcctgggagc gcttcggcgc gctcgccggc gcgctcgacg aggggtcgcg cgcgctgctc
      661 gccaccgtgc cgtcggcgcg ccggcccggc gcgccgctcg acgcgtgcgt ggccgggttc
      721 cttgccggca tcgcggcggc cagggacacc ctgcctggct gggccctgcc cgagctggcc
      781 ggcgcccagg cggccaccgc ggccgccctg gacgcggccg agcgacgggc ccgcgggctc
      841 gcggccgcca ccgaggggct cgagttcgag caccgcaacg cggccgtcgc cgaggtgctg
      901 gacgagctcg ccccggtcca ggacgccgag gtcgccctgc ggcggctccg ccgccgcgcg
      961 gcccgctcgc gccgctgagc gtccagccgg gctcggctcg gaagcacggc gcgtcttgac
     1021 gggccgctta cccgcgttcg gtgagagtgc tcccgaacgc cgggatcgcc gcaccccggc
     1081 cgccggaccg cccgctggag ccgcccgatg accttcgcca cccctgtcct gctgctcggg
     1141 ctgctgctgg tcccgctcgt cctgctcgcc ctggtgctcg cacagcgcag gggggtgcgc
     1201 tacgcggtcc gctatcccgc cttggacgtg ctcgctgggg tcgtcgggcg gacgcccggc
     1261 tggcgtaggc acctccccgc ggcgctgctg ctgctcgcgc tcacggccct gctgctcggt
     1321 acggcgcggc cgatggcgcg ggtgcccgtc cccagggacc aggccacggt gctgctcgtg
     1381 gtcgacgtgt ccgggtcgat gaacgccgac gacgtcgacc cgacccggct cggggcggcc
     1441 cgcgcagccg cgggccggtt cctcgaccgg ctgcctgagc gcttccaggt cgggctggtc
     1501 gcgttcgcga gcggcgccca gacgctggtc cggccgacca ccgaccgcgc cgcggtccgc
     1561 caggcgctcg agacgctgca ggcgggcggg gggaccgcga tgggcgacgg gctcgcccgc
     1621 gcgctcgaca gcgtcgaggc ggcccgccgg gccgcgggcg gcggcgcgaa gcgggccacc
     1681 ccgggccgat cgggacaggc agcgtcgccc cccgcgagcc ggccggcggt cgcgctgctg
     1741 ctgtcgg
//
LOCUS       DASPYY010000463         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2467532, whole genome shotgun sequence.
ACCESSION   DASPYY010000463 DASPYY010000000
VERSION     DASPYY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467532"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..558
                     /locus_tag="VEP73_07260"
     CDS             109..558
                     /locus_tag="VEP73_07260"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYT26264.1"
                     /translation="MLTDDVQRAMYAAMKAHEADEAAALRLVLSALKSAAIDARRDLT
                     DEEAVAVLQREVRKRREAERAYRDAGRADRAQREAFELGVVSRFLPAGLEPAELDRLV
                     EEAVTATGATGPKDLGKVMGRLMPAVRGRAEGAEVRRLVLERLQGGP"
     gene            complement(571..>1929)
                     /locus_tag="VEP73_07265"
     CDS             complement(571..>1929)
                     /locus_tag="VEP73_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYT26265.1"
                     /translation="TSGRIIAMYGGKSFKDNQGNLAVLGQGSDGYQSGSTFKVFYLVS
                     ALEQGIPTSQTFNAPPQINIRYPLCGSKGWDVANAEKSEAGLYNMYTATSHSVNTYFA
                     QLMPMVKPSRAVEVARRMGIQVPTKGSLAYANHWNICSSVLGTGNTSVLDMASAFAVL
                     ANKGVRCEPYSIAKVVDRDNDVLLDRTRSKPRCEQVVEPGIAAQVTDILRGVVSGGTG
                     VAANIGRPVAGKTGTAQGYTSAFFTGYTPQLATSVWVGHRKGLRSMYVNGRPVYGGTI
                     PAPIFHDFMSSAMAGLPVVGFPPPPHGRPQDPASKPGVGVPQVTGQQLAQALRTLAAA
                     GFKVSVRFVDSQAPKNQVVAQSPAAGGTVPGNSQVTLLVSKGGGGGGGQATTVVPAVQ
                     GLRAKAAAALLRAAGFVARAFYAPIADATLVGRVISQSPAAGSQWRSGGTVMLLVGRA
                     AF"
BASE COUNT          257 a          675 c          718 g          279 t
ORIGIN      
        1 gacgaaccac gcatacgtct cgagcctccg aatcgctgtc tggtcgggtg gagattgtag
       61 cggcgcggta gggcgcgggc gcgatccggg ttcgggtagc ctcggggcat gctcaccgac
      121 gacgtgcagc gagcgatgta cgcggccatg aaggcgcacg aggcggacga ggcggccgcc
      181 ctccggctgg tgctgtcggc gctcaagtcg gccgccatcg acgctcgccg cgacctgacc
      241 gacgaggagg ccgtcgcggt cctccagcgc gaggtgagga agcgccggga ggccgagcgg
      301 gcctaccggg atgccggccg ggccgaccgg gcccaacgcg aggcgttcga gctcggcgtc
      361 gtctcccggt tcctgcccgc cgggctcgag ccggccgagc tggaccgcct cgtcgaggag
      421 gcggtgaccg ccaccggcgc caccgggccg aaggacctcg ggaaggtcat ggggcggctg
      481 atgccggcgg tcaggggacg ggccgagggc gccgaggtcc gccggctcgt gctcgagcgt
      541 ctccagggcg ggccgtagcc ggcggccggg tcagaaggcc gcgcgcccga cgaggagcat
      601 gaccgtgccg ccgctccgcc actgggagcc ggccgccggg ctctgcgaga tcacccggcc
      661 gaccagggtc gcgtcggcga tcggggcgta gaaggcgcgg gcgacgaagc cggcggcgcg
      721 caggagggcc gctgccgctt tggccctgag cccctgcacc gccgggacga ccgtcgtggc
      781 ctggccgccc ccgcccccgc cgcccttcga gaccaggagg gtgacctgcg agttcccggg
      841 gaccgtgccg ccggccgccg ggctctgggc gaccacctgg ttcttcggcg cctggctgtc
      901 gacgaaccgc accgacacct tgaagcccgc ggccgcgagg gtccgcagcg cctgcgcgag
      961 ctgctggccg gtcacctgcg gcacgcccac gcccggcttg gacgccgggt cctgcggccg
     1021 gccgtgtggt ggaggcggga agccgaccac gggcaggccg gccatggcgc tcgacatgaa
     1081 gtcgtggaag atgggtgccg ggatggtccc cccgtagacc ggcctcccgt tgacgtacat
     1141 ggaccgcagg cccttccggt gcccgaccca gaccgaggtg gcgagctggg gcgtgtaccc
     1201 ggtgaagaag gcgctcgtgt agccctgcgc ggtccccgtc ttgcccgcga ccgggcggcc
     1261 gatgttcgcc gcgacgccgg tacccccgct gaccacgcca cggagtatgt cggtgacctg
     1321 ggccgcgatc ccgggctcga cgacctgctc gcacctcggc ttcgacctcg tccggtccag
     1381 caggacgtcg ttgtcccggt cgacgacctt ggcgatggag tacggctcgc aacggacgcc
     1441 cttgttggcg agcaccgcga aggcgctcgc catgtccagc acgctggtgt tgcccgtgcc
     1501 gagcaccgac gagcagatgt tccagtggtt cgcgtacgcg agcgagccct tcgtgggcac
     1561 ctggatcccc atccgcctcg ccacctcgac cgccctgctc ggcttgacca tgggcatgag
     1621 ctgcgcgaag taggtgttca cggaatggga ggtcgccgtg tacatgttgt agaggccggc
     1681 ctcggacttc tcggcgttgg ccacgtccca gcccttcgag ccgcacaggg ggtagcggat
     1741 gttgatctgg ggcggggcgt tgaacgtctg gctcgtcggg atcccctgct ccagcgcgct
     1801 caccaggtag aagaccttga acgtcgagcc cgactggtag ccgtccgagc cctggccgag
     1861 gacggccagg ttcccctggt tgtccttgaa gctcttgccg ccgtacatgg cgatgatcct
     1921 gccgctggt
//
LOCUS       DASPYY010000464         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2467592, whole genome shotgun sequence.
ACCESSION   DASPYY010000464 DASPYY010000000
VERSION     DASPYY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467592"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..806)
                     /locus_tag="VEP73_07270"
     CDS             complement(273..806)
                     /locus_tag="VEP73_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26266.1"
                     /translation="MRVLFVCTGNICRSPIAEAIAWRELARYPDATVQFSSAGSHALE
                     GNPATGRAVAAAAALGADLEWHRARELTRRRVQDADLVLCMAAEHLPFVAAYERAAAD
                     RAFSLAAFARAVATVDRMAVTPEELVSMAAKAITDEPGDDVEDPIGLSSDAYAACARR
                     LDELVPVVVRALARVSS"
     gene            complement(909..1601)
                     /locus_tag="VEP73_07275"
     CDS             complement(909..1601)
                     /locus_tag="VEP73_07275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYT26267.1"
                     /translation="MTDGDDVLADEPRVVRVTSDERLWDGLLEVGGKVVAGGGLVVVP
                     TDTVYGVGCDPFNASAVDALFRAKERGRDLPLPILVHGWRQAVGLVDEVTEQAKALIA
                     AWWPGPLSIVLREAAGIGWDLGDSRGTVMVRMPKQTFTLALIQRTGPLAVSSANRSGL
                     PTPDRMPGIVEQLGDAVGVFFDAGPAEAGPASTIVDLTGPFPRLLRRGAVPEQEIERV
                     LEEPLVDATRSG"
     gene            complement(1628..>1903)
                     /locus_tag="VEP73_07280"
     CDS             complement(1628..>1903)
                     /locus_tag="VEP73_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYT26268.1"
                     /translation="VPTAAIDLLPRDVREREPRLALDGGPDGLDVLRAVAARAPRWLA
                     PGGALLCEIGDDQGGPAVRVLEAAGFRDVGVHPDLAGRDRVVLGVRP"
BASE COUNT          253 a          729 c          694 g          227 t
ORIGIN      
        1 ggccgacgcc gaccggtggg cccacacgat caccccagcc aaggtgctgt cgcagatcgg
       61 cagcccgttc gcacccgacc agcacccgcc tgtcggggag gtactcaacc atgcagcatg
      121 aactcccagg aagcatttag cggccggcct ggtcggggcc gtagaaggtc atggggagtc
      181 cctttcgccc atcgtcgccg ttgccgagcc tgctcgaggc tatcacgcgc cccgatccgc
      241 cgacgccggg agcgccgatc aggagaccct ggtcaggagg agaccctggc gagcgcgcgg
      301 accacgacgg gcacgagctc gtcgagcctt ctcgcgcagg ccgcgtacgc gtcgctggac
      361 agcccgatgg ggtcctcgac gtcgtcgccc ggctcgtcgg tgatcgcctt ggccgccatg
      421 gacacgagct cctcgggcgt cacggccatc cggtcgaccg tggccacggc gcgggcgaag
      481 gcggcgagcg agaacgcgcg gtcggccgcc gcgcgctcat aggcggccac gaacgggagg
      541 tgctcggcgg ccatgcagag caccaggtcc gcgtcctgga cgcggcgccg ggtcagctcg
      601 cgggcgcggt gccactcgag gtcggcgccg agcgcggcgg cggcggccac cgcccggccc
      661 gtggccgggt tcccctcgag cgcgtggctg ccggccgagc tgaactgcac ggtcgcgtcc
      721 gggtagcggg ccagctcccg ccaggcgatg gcctcggcga tcggcgaccg gcagatgttg
      781 ccggtgcaga cgaagaggac ccgcacgtcg ggctcgtcga gcacgccgcg gagccgccgt
      841 gggttaccca gcctcggcca cctcctcccg ggccgcgcga tggtcggcct cccgccatgg
      901 tggcaccgtc agccggacct cgtcgcgtcc acgagcggct cctcgagcac ccgctcgatc
      961 tcctgctccg ggacggcacc ccggcgcagc agccggggga aggggccggt caggtcgacg
     1021 atcgtcgacg ccgggccggc ttcggccggg ccggcgtcga agaacacgcc gaccgcgtcg
     1081 ccgagctgct ccacgatgcc gggcatgcgg tccggggtgg gcaggcccga acggttggcg
     1141 ctcgacaccg cgagcggccc ggtccgctgg atcagggcga gcgtgaaggt ctgcttgggc
     1201 atgcgcacca tcaccgtgcc ccgggagtcc ccgaggtccc atccgatccc ggccgcctcg
     1261 cgcagcacga tcgagagcgg gccgggccac caggccgcga tgagtgcctt cgcctgctcg
     1321 gtcacctcgt cgacgagccc gaccgcctgg cgccagccgt gcaccaggat gggcagcggc
     1381 aggtcgcggc cgcgctcctt ggcgcggaac agggcgtcca cggccgaggc gttgaacggg
     1441 tcgcagccga ccccgtacac ggtgtcggtc gggaccacga cgaggccgcc gccggcgacg
     1501 accttgccgc caacctcgag caggccgtcc cacaggcgct cgtcactcgt gacccggacg
     1561 accctcggct cgtcggccag cacgtcgtcc ccgtcggtca tggtcgcctc cctcgtggtc
     1621 gcacccctca tggtcgcacc cccaggacca cgcggtcccg gcctgccagg tcggggtgca
     1681 cgccgacgtc ccggaagccg gccgcctcga gcacccgcac ggcgggcccg ccctggtcgt
     1741 caccgatctc gcagagcagc gcgccgccgg gcgcgagcca gcgcggcgcc cgtgcggcca
     1801 cggcgcgcag gacgtcgaga ccgtcggggc cgccgtccag ggccagccgc ggctcgcgct
     1861 cgcgcacgtc gcggggcagg aggtcgatgg ccgccgtcgg cac
//
LOCUS       DASPYY010000465         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2326300, whole genome shotgun sequence.
ACCESSION   DASPYY010000465 DASPYY010000000
VERSION     DASPYY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326300"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VEP73_07285"
     CDS             <1..990
                     /locus_tag="VEP73_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26269.1"
                     /translation="ASAPAKAMQALHRSSVTTGDLRGYERVTSGFARNYATRLDGGPV
                     ALVAPMLAHLHTLLLALEQPQPPNTRRRLCVQVTQLNVLLGTAAFDLNNLFVARSYYG
                     SALVAAEQSEEAALCADVLVKTAWAVVGRSTRSVRYADQPERTAARAREALELLGQAQ
                     GFADRDASPWTRAQLAGLRAEAYASLGQKQATEASLQQAHRAADGARPGARRYPIEFY
                     DQARLASSEGLCYLRLHEPKLAQAALRASQALQGPTMLINRSIVQLDLAITSFQLRDV
                     DQACEFGDQIPDVGPTHQVIAVRQHTQDLRRELAPYRSVRAVRELDERLRAIA"
     gene            987..1943
                     /locus_tag="VEP73_07290"
     CDS             987..1943
                     /locus_tag="VEP73_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26270.1"
                     /translation="MTPRPNPSMLRDYETMTMQLARAYFTRLDEGPEVLALPIELQLD
                     LADRLLERPQRAADRRRLCAIGAQLSMLLGIATYDLGQPTPAQGWFDAAASAAARAGE
                     PALGAEALASAAWAALGRSTRGLWEADDRQQTQADVWHAVGLLDRAETLAARDAIPWT
                     QARLASLRAEASATLGDVAATEANLATAHRAIEQARPGQRRWGIVFFDAARLAFSEGL
                     CELRLHRPVRAQAALRASIGLQGPCSFVNRSVAQLGLAIAHARHGEVEHACEIGAGVG
                     PIPPTHRVTAIREHARDLRRELRPHLGLPAAHVLDERLTAIA"
     gene            2238..2965
                     /locus_tag="VEP73_07295"
                     /pseudo
     CDS             2238..2965
                     /locus_tag="VEP73_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
BASE COUNT          415 a         1214 c         1054 g          411 t
ORIGIN      
        1 gccagcgcgc cggcaaaggc catgcaggcg ctccacaggt cgagcgtcac caccggcgac
       61 ctccgcggct atgagcgggt gaccagcggc ttcgcgcgca actacgccac gcggctcgac
      121 ggcggcccgg tcgccctcgt cgctccgatg ctggcgcatc tgcacacgct gctcctggcc
      181 ctcgagcagc cacagcctcc caacacccgg cggcggctct gcgtgcaggt gacacagctg
      241 aacgtgctgc tcgggaccgc cgcgttcgat ctcaacaacc tgttcgtcgc gcggagctac
      301 tacggcagcg cgctggtcgc cgccgagcag tccgaagagg ctgccctgtg cgccgacgtg
      361 ctggtcaaga ccgcttgggc ggtggtcggc aggtcgaccc ggagcgtgcg gtacgccgac
      421 cagccggagc gcaccgcggc cagggcgcgc gaagccctgg agctgctcgg gcaggcgcag
      481 ggcttcgccg atcgggacgc cagcccctgg acccgcgccc agctcgccgg gctgcgtgcg
      541 gaggcatacg ccagccttgg gcagaagcag gccaccgaag cgagcctgca gcaggcccac
      601 cgggccgccg acggcgcacg tccaggtgcg cgccgctacc cgatcgagtt ctacgaccag
      661 gctcgcctcg cgtcctcgga agggctgtgc tacctgcgcc tgcacgagcc gaagctggcc
      721 caggcggcgc tgcgggcgtc gcaggccctg cagggaccga cgatgctcat caaccgctcg
      781 atcgtgcagc tcgacctcgc catcacctcg ttccagctgc gcgacgtcga ccaggcctgc
      841 gagttcggcg accagatccc cgacgtcggc ccgacccacc aggtcatcgc ggtcaggcag
      901 cacacccagg acctccgccg cgagctcgct ccctaccgga gcgttcgagc cgtccgcgag
      961 ctggacgagc gactgagggc gatcgcgtga cgccccgccc gaacccgagc atgctgcgcg
     1021 actacgagac gatgaccatg cagctcgcgc gtgcctactt cacccgcctg gacgagggcc
     1081 ccgaagtgct cgccctgccg atcgagctcc agctcgacct ggcggaccgg ttgctggaac
     1141 gaccacagcg ggccgccgac cgtcgccggc tgtgcgcgat cggcgcgcag ctctccatgc
     1201 tgctgggcat cgccacctac gacctcggcc agcccacgcc cgcccagggc tggttcgacg
     1261 cggcggcgtc ggctgccgcg cgggcgggcg agcccgcgct cggtgccgag gcgctggcca
     1321 gcgcggcctg ggcggcgctc ggccgctcca cccgcggcct ctgggaagcc gacgaccgcc
     1381 agcagacgca ggcggacgtc tggcacgccg tgggcctgct cgatcgagca gagacgctcg
     1441 ccgcacggga cgccatccca tggacccagg ccaggctggc atcgctgcgc gccgaggcgt
     1501 cggccacgct cggggacgtc gccgccaccg aggcgaacct cgcgaccgcc caccgcgcga
     1561 tcgaacaggc ccggcccggg cagcggcgct ggggaatcgt gttcttcgac gcggcccgtc
     1621 tggccttctc cgaagggctc tgtgagctgc gcctccaccg gcccgtacgg gcgcaggcgg
     1681 cgctgcgggc gtcgatcggc ctgcaggggc catgctcgtt cgtcaaccgg tccgtcgccc
     1741 agctcggcct cgccatcgcg cacgcccgcc atggcgaggt cgagcacgcc tgcgagatcg
     1801 gcgccggagt cgggccgatc cccccgaccc atcgggtcac ggcgatcaga gagcacgccc
     1861 gtgacctgcg ccgggagctg cgcccccatc tgggcctgcc ggcggcgcac gtcctcgacg
     1921 agcgcctgac cgcgatcgca tgacccgttc accctcgtcg ccctgcgccg cttcgcctgg
     1981 acgcccgcgg ccgtgaccgc ctgagccccg cgctcgcccc ggcctctcgc ataccccgtg
     2041 ctacggtgtg gcccatgcga accagcttcc tctgacgctc acgcgctcgg tctcctccct
     2101 cggcccggac ggcccacgac ccgttcgagg gaggagatca tcgtgctcgg caatgaggag
     2161 aatcccgcct atgggttcga cgacgacgag gccacccggc gactgctgcg ctcccgcccg
     2221 ccccgcgagg ctctcgcctg ggccggctcg gcgctcggcg gcgccgtcgt cgccgcccgg
     2281 cccctgcgag gcgggagggc cggacccttc gcttcgtcga gaccctggac ctgccggcgc
     2341 cacgcctgct gaccctggac ccgaccggga gcgaggccgg cacgccggcc gtgctgatgt
     2401 cgcgtctccc ggggcgggtc gagtggtccc ccaccgacat ggaccggtgg ctgcgggatc
     2461 tcgccagcct cctcccggtc atccattcgg cgccgatgcc ctgcccggcg tgatccagcc
     2521 gttcgccccg tacgcacaga ccagctacga gccgccggcg tgggctcgct ggcccgcgac
     2581 atgggagcgg gcggtcgagc tgttccacgc acccaccccc gagggtgccg aggccttcac
     2641 tcaccgtgac ttccaccccg ggaacgtgct gtggcgccgc ggtagggtga ccggggtggt
     2701 ggactgggcc tcggcgagta tcggcccggc ctgtatcgac gtcggccact gccgcgccaa
     2761 cctgttcccg tacggcctcg acgtggccga ccgcttcacc gtcatatggg agcaggtcag
     2821 cggcgcccgc tacaacccgt gggccgacgt cgtcaccatc atcggctgcc tcgacgggct
     2881 gcgcgacgac ccgcccccgg agcgctccgt cgtcgaggac gtcctggcca ggtcggtcgc
     2941 cgacctcctc ggcaccccga tgtgacggcc gcgcgttctt cgccttcctc ccttgccagg
     3001 acgacgagaa gccagcccag ccccaagacg aagcgaaggc gggcgctcac ccgggctcgg
     3061 ccgctcgggg tgggatggcg tagccaagct cgtg
//
LOCUS       DASPYY010000466         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2185782, whole genome shotgun sequence.
ACCESSION   DASPYY010000466 DASPYY010000000
VERSION     DASPYY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185782"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..2013
                     /gene="lysS"
                     /locus_tag="VEP73_07300"
     CDS             475..2013
                     /gene="lysS"
                     /locus_tag="VEP73_07300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYT26271.1"
                     /translation="MPDERPAAPLPGGVPDGTSESDWSALERVRREKLARLRAAGVDP
                     YPVGFARSHTLAEVARHWPDLAPGQESGDVVRVAGRIVLKRDFGRLAFWTLRHGGDEL
                     QAMLTVDSLGPDQLELVTDLDAGDWVGVQGEVVRTRRGELSVKATEVFLLAKSLRPLP
                     DKWKGLTDVEVRSRQREVDLTVNAAARAAMLLRSRVVQSVRQSMVARGYLEVETPILQ
                     PLPGGALARPFVTHHNALDTDLYLRVAGELYLKRLLVGGVDRVFELNRMFRNEGIDAR
                     HNPEFTMMESNEAFADHRDMMRLTQAVVRESADAALGTRTVHYQGATIDLDAEWREVT
                     LLDAVRDAVGEPDLAYDWPLDKVRAACDRCGVPYEPGWGTGKLVLELYEKHAEHRIGP
                     PTFVTDYPVEVSPLAHPHRDDPFVTERFELIVLGREYANAFSELTDPDEQRRRMEEQA
                     LARAGGDEDAMVVDDAYLRALELGLPPNAGLGIGVDRLVMLLGDLASIREAVLFPQLR
                     PEER"
     gene            2221..>2448
                     /locus_tag="VEP73_07305"
     CDS             2221..>2448
                     /locus_tag="VEP73_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYT26272.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRAQVEEIIGQGQTAPTG"
BASE COUNT          350 a          879 c          888 g          331 t
ORIGIN      
        1 gcctcggggt gctcggaggg ctggtcctcg tcgtctacgg gagagccgcc ggtctgggtc
       61 cgcccgccca ggtgctcggc gggctcctgc tgctcggctc gctggcggcg ctggtcgcgg
      121 ccaggccccg ctgaaccgcc gggccgcttc gcccctgggt gccagccgca gccgcgcacc
      181 ggctgcgttg gcaggtgcgg cgggaggccg agacaggccg gagaatcatt gtcctggggg
      241 aggtggcctg ccctccgtcc cgcctgcacc gtcagctctt atggtatgac ggcgtgcgtt
      301 gacgcaaacc tggagattgt gcgtacgtac tttcgtcctc gccggcctgg caccggatga
      361 ccatgcccga ggcgggcgcg gctggcgcgg tccagtagca tcgggttcga ccacgcgtga
      421 cggccgcgtg acggccgacc caggaagacg agcgacccga ggaagaggag cagcgtgccc
      481 gacgagcgac ccgcagcgcc cctgcccggc ggcgtgccgg acgggacaag cgaatcggac
      541 tggtccgccc tcgagcgggt gcggcgcgag aagctggcca ggctccgggc ggccggcgtc
      601 gacccctacc cggtcggctt cgccaggagc cacaccctcg ccgaggtcgc ccgccactgg
      661 ccggacctcg cccccgggca ggagtccggc gacgtcgtcc gggtcgccgg gcgcatcgtg
      721 ctcaagcggg acttcggcag gctcgcgttc tggacgctgc gccacggcgg cgacgagctc
      781 caggcgatgc tgaccgtgga ctcgctcgga cccgaccagc tcgagctcgt gaccgacctc
      841 gacgccggcg actgggtggg tgtccagggg gaggtggtcc gcacccgcag gggcgagctg
      901 tccgtcaagg cgaccgaggt gttcctcctg gccaagtcgc tgcggcccct gccggacaag
      961 tggaagggcc tcaccgacgt ggaggtgcgc tcgcggcagc gggaggtcga cctgaccgtc
     1021 aacgcggccg cccgcgccgc catgctgctg cggtcgcggg tggtgcagtc ggtcaggcag
     1081 tcgatggtcg cgcgcggcta cctcgaggtg gagacgccga tcctgcagcc actgcccggc
     1141 ggcgccctcg cgcgcccctt cgtcacccac cacaacgcgc tcgacaccga cctctacctg
     1201 cgggtcgctg gcgagctgta cctcaagcgc ctgctcgtcg ggggcgtcga ccgggtgttc
     1261 gagctgaacc ggatgttccg caacgagggc atcgacgcgc gccacaaccc cgagttcacg
     1321 atgatggagt ccaacgaggc gttcgccgac caccgcgaca tgatgcggct gacccaggcg
     1381 gtcgtccgcg agagcgccga cgcggcgctg ggcacccgca cggtccacta ccagggggcg
     1441 acgatcgacc tcgacgccga gtggcgcgag gtgacgctgc tcgacgcggt ccgcgacgcc
     1501 gtcggcgagc ccgacctcgc ctacgactgg cccctcgaca aggtcagggc cgcctgcgac
     1561 cgctgcggcg tcccctacga gcccggctgg gggaccggca agctcgtgct cgagctgtac
     1621 gagaagcacg ccgagcaccg catcgggccg cccaccttcg tgaccgacta cccggtcgag
     1681 gtgtcgccgc tggcccatcc ccaccgcgac gacccgttcg tcaccgagcg cttcgagctc
     1741 atcgtcctcg ggcgcgagta cgccaacgcc ttctcggagc tcaccgaccc cgacgagcag
     1801 cgcaggcgca tggaggagca ggcgctcgcc agggccgggg gggacgagga cgccatggtg
     1861 gtcgacgacg cctacctgcg cgcgctcgag ctcgggctgc cgccgaacgc cggcctcggc
     1921 atcggggtcg accggctcgt catgctgctc ggcgacctgg cgtcgatccg cgaggccgtg
     1981 ctgttcccgc agctccgccc cgaggagcgc tagccgggga gcggccggcc ggtcaagcga
     2041 cggcgtccgg cgccgatgaa tcagcgacga tccgggaaca gaaggtgacg ccggtgcgtt
     2101 gagccactcg acagagcacg tccggtcgac ggcggaccgc ccgtcgggac gccagaggcc
     2161 cagtgctata gtcgattggc ggcaagtacc gctgccgccg tatccctggg ggtttggccc
     2221 atgtttgagc gcttcaccga ccgggcgaga cgagtcgtcg tgctcgctca ggaggaggct
     2281 cgactcctca accacaacta catcgggacc gagcacatcc tgctcgggct gatccacgag
     2341 ggcgagggcg tcgccgccaa ggcgctcgag tcgctcggca tctcgctgga ggcggtccgc
     2401 gcccaggtcg aggagatcat cggccagggc cagacggcgc cgaccggg
//
LOCUS       DASPYY010000467         7365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2468135, whole genome shotgun sequence.
ACCESSION   DASPYY010000467 DASPYY010000000
VERSION     DASPYY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468135"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VEP73_07310"
     CDS             complement(<1..1584)
                     /locus_tag="VEP73_07310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYT26273.1"
                     /translation="MHVLAQSAAETPLALPPIDWRAIAPELALAAAAVVLLLVVAWWP
                     NARRGVLAALTLAGLAVAAGLTAWNWDMAKTAFEGAVAIDGITRYARVVLLAAGALAT
                     LMAASEGARDPEEPPEIFPLLLFALTGMVLLAAASDLLVVFIALEMLSLSLYVMAGAT
                     RRLRAQEAAMKYFLLGAFSSAFLLYGIALCYGATGTTNLLRIAQATGDPATSSTLLLA
                     GAGLLSVGFLFKVAAVPFHMWTPDVYQGAPTPVTAFMAAATKTAGFAALLRVFAGSLG
                     GLVLSWRPAFAAVAVVTMLVGAVLAVVQSDVKRMLAYSSISHAGYLLIGVIAAATRPE
                     GVSASMFYLLAYAFMVMGAFMVVQYASPPRRAAAATAAEAARGVPAAAAVGAVAARGA
                     SSGPGVAAAAEAAGRSGGTAPVITMTREAPREATAPTVPAGEPDAVEERSSLDDYRGF
                     GRRHLGPALLLAVFLFALAGIPPFSGFWAKYYVFQAGIDAGLTWLVVVAVVSSIISVF
                     FYLRVIVVAFLQEPAGDDPG"
     gene            complement(1637..3193)
                     /locus_tag="VEP73_07315"
     CDS             complement(1637..3193)
                     /locus_tag="VEP73_07315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYT26274.1"
                     /translation="MLLTLTVFAPLAGALLVAAIPRARASGGPGEPATAAPLDRAVRW
                     VALLASLVTLGLAVALAARFRTGEAGFQLGQHANWVRSFGVQYKVGVDGVSLPLVMLT
                     TVLVPLAIAGSWRMTDRPRNYLALLLVLETAMLGVFAALDMFLFYIFWEAVLIPAYFL
                     IGSWGGERRVYAAVKFFIYTLFGGLLMLVGIVSLYYLKSRAAGPGTFDYEALRDLSKD
                     PTTQRWLFGAFFAAFAVKTPLVPFHTWLPDAYAEAPTTTTVLLAGVMSKLGIYGFLRF
                     ALPLFPDAARYFQPALMTLAVVGILYCAVIAAVQKDFKRLVAYSSVSHLGFIVLGIFA
                     FTVQGLQGGVFYMVAHGIVIGGLFFVTGMLEERRATRRIEDFGGLQQSMPRMAGVMLV
                     ITLASLALPGLVGFVGEFLTLLGAFLAHPVYAVLGAIGVILGAVYLLWAYQRMWQGPL
                     TSEANRAIPDLSAREWAILAPLVAAIVVLGLFPRPVLERVEPSVQRVVQQTARPAGQG
                     AALAPSQPSP"
     gene            complement(3204..5114)
                     /gene="nuoL"
                     /locus_tag="VEP73_07320"
     CDS             complement(3204..5114)
                     /gene="nuoL"
                     /locus_tag="VEP73_07320"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYT26275.1"
                     /translation="MNATGTIRLVWLLPVLPLAGFAVNAALGQRLRALAGWLATALVA
                     ASFAIAVAAFLALLSHSGDERTQVVHGYGWITSGSLRVAFDLRWDQLSATMALVVTGV
                     SSLIHLYSIGYMADDGRRETFFSWLNLFVAAMLVLVLAQNFLVMFIGWEGVGLCSYLL
                     IGFWFNREGVPAAAKKAFVANRIGDVGFLIAMFLIFQKFGSLDFSDVLGRVGSLRGGT
                     ATAIALLLFLACTGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVFLVARTHVL
                     FEASGTAQTVVVVIGALTALVAALIAVGQDDIKRILAYSTVSQLGYMFIGVGLGPIGY
                     AAGLFHLVTHAFFKAQLFLGAGSVMHGNDDDTDVKRFGGLRRAMPVTFLTMGAAWLAI
                     IGFPLTSGFFSKEAILGAAFAHGGAGLFAWVVGVLTAGLTAFYMSRLYFLTFFGEPRW
                     PSGRHPHEAPAVMTVPLLVLAVLALVGGVLGGFSGVGAIGRFLEPVLGRAPEESRSFL
                     AGFLLSLAAVGAAAVGALAAWWVYLSGRVEWAALRVRYAPAWRLLANKLYVDDVYEFF
                     TVTLGRALAAFLAVNVDQRAIDGAVNGAAEAVGAVAGRGRRLQSGLVRTYAVGVLGGA
                     VLIIAFLVFRTT"
     gene            complement(5122..5427)
                     /gene="nuoK"
                     /locus_tag="VEP73_07325"
     CDS             complement(5122..5427)
                     /gene="nuoK"
                     /locus_tag="VEP73_07325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYT26276.1"
                     /translation="MKVGTNAYLLLSALLFTIGVVGVLIRRNAIVVFMCVELMLNAVN
                     LSLVAFARRNGTLDGQVLAFFVMVVAAAEVVVGLGILVAIFRKRSSISVDDVNLLKG"
     gene            complement(5424..5963)
                     /locus_tag="VEP73_07330"
     CDS             complement(5424..5963)
                     /locus_tag="VEP73_07330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYT26277.1"
                     /translation="MIAVAATTAADRAELVTFVPLALLSIVAAVGMVLSRNAVYSALL
                     LVVNFFCLAGFYVLLQAQFLAAVQVIVYAGAVMVLFLFVLMLLGIGNEDVLRETIPGQ
                     RTIAGVLGVLLLVAVVVAVLNGGLSGPAGDLAAANQSGNTQAVGRLLFTRYVFAFEAT
                     GILLVVAAVGALVLGKRRP"
     gene            complement(6116..6559)
                     /gene="nuoI"
                     /locus_tag="VEP73_07335"
                     /pseudo
     CDS             complement(6116..6559)
                     /gene="nuoI"
                     /locus_tag="VEP73_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006866506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            complement(6559..>7365)
                     /locus_tag="VEP73_07340"
     CDS             complement(6559..>7365)
                     /locus_tag="VEP73_07340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HYT26278.1"
                     /translation="LAMGSLQVYGIVLAGWASGSNYPLLGAVRSSAQMISYEIAQGLS
                     IVGVLMYAGTLSASGIVAAQHGNPIWGVFPRWYIVPQLPAFFVFLLAGIAETNRAPFD
                     LPEAETELVGGYHTEYSGVKFALFFLAEYLNVITVSAVATTLFLGGPDGPTIHGFLPW
                     LWPILWFVVKIYLFIFVFIWLRATLPRLRFDRLMAIGWKFLIPVALAWIAVTAVVLTV
                     NLSSPGRRVLFIGIGILLVLLLVSFLVPSPKESRPKPGAPDRTAPGGSTP"
BASE COUNT         1284 a         2436 c         2835 g          810 t
ORIGIN      
        1 gccagggtcg tcgccggccg gctcctgcag gaacgcgacg acgatgaccc gcaggtagaa
       61 gaagacggag atgatggagc tgaccaccgc cacgacgacg agccaggtga gcccggcgtc
      121 gatgcccgcc tggaagacat agtacttggc ccagaacccc gagaacggcg ggatgccggc
      181 gagcgcgaac aggaacacgg cgagcagcag ggccggcccg aggtggcggc gcccgaagcc
      241 gcggtagtcg tcgagcgacg agcgctcctc gaccgcgtcc ggctcgccgg ccggcacggt
      301 gggggcggtg gcctcccggg gcgcctccct ggtcatggtg atgaccggcg cggtcccacc
      361 cgagcggccg gcggcctccg cggcggcggc cacgccgggc ccggaggagg cgccccgggc
      421 ggccaccgcc cccaccgcgg cggcggccgg cacgccccgg gcggcctcgg cggccgtcgc
      481 ggcggcggcc cggcgcggcg gcgaggcgta ctgcaccacc atgaacgcgc ccatgaccat
      541 gaaggcgtag gcgagcaggt agaacatgga cgcgctgacg ccctccggcc tggtcgcggc
      601 ggcgatcacg ccgatcagca ggtacccggc gtgggagatc gacgagtagg cgagcatgcg
      661 cttcacgtcg gactggacga cggcgaggac cgcgccgacc agcatcgtga ccaccgcgac
      721 ggctgcgaag gcggggcgcc atgacagcac cagcccgcca agggagccgg cgaagacccg
      781 caggagggcg gcgaagccgg ccgtcttggt ggcggccgcc atgaacgcgg tcaccggggt
      841 gggcgcgccc tggtagacgt cgggcgtcca catgtggaac ggcaccgcgg ccaccttgaa
      901 caggaacccg accgacaaga gccccgcccc cgcgagcagc agggtcgagc tggtggcggg
      961 gtcgccggtg gcctgggcga tgcggagcag gttcgtcgtc ccggtcgccc cgtagcacag
     1021 ggcgatcccg tagagcagga aggccgacga gaacgcaccg agcaggaagt acttcatcgc
     1081 cgcctcctgc gcgcgcaggc ggcgggtggc cccggccatc acgtagaggc tgagcgagag
     1141 catctcgagg gcgatgaaca ccaccagcag gtcggaggcg gcggccagga gcaccatgcc
     1201 ggtgagcgcg aacagcagga gcgggaagat ctccggtggc tcctcggggt cccgggcgcc
     1261 ctcgctggcc gccatcagcg tggccagcgc gcccgcggcg aggagcacca cccgggcgta
     1321 gcgggtgatg ccgtcgatcg cgacggcgcc ctcgaaggcg gtcttcgcca tatcccagtt
     1381 ccacgccgtc agcccggccg cgacggccag gccggcgagg gtgagcgcgg cgagcacgcc
     1441 gcgcctggcg ttgggccacc acgccaccac gaggagcagg accaccgcgg cggcggccag
     1501 ggcgagctcg ggcgcgatcg cgcgccagtc gatcggcggc agggcgagcg gcgtctcggc
     1561 ggcggactgg gccaggacgt gcacgggctc ctccagggga tccggggcgg gccggtgggg
     1621 ccgccctggc gggcggtcag gggcttggct ggctgggcgc cagggcggcg ccctggccgg
     1681 ctggtcgcgc cgtctgctgg acgacgcgct ggacggacgg ctcgacccgc tcgaggaccg
     1741 ggcgcgggaa caagccgagc accacgatcg ccgccacgag cggcgcgagg atggcccact
     1801 cccgggcgga gaggtcgggg atggcgcggt tcgcctccga ggtgagcggg ccctgccaca
     1861 tgcgctggta ggcccagagc aggtacaccg cccccaggat cacgccgatc gcgccaagca
     1921 cggcgtagac cgggtgggcg aggaaggcgc cgaggagggt gaggaactcg ccgacgaacc
     1981 cgaccaggcc gggcagggcg agcgaggcga gggtgatgac cagcatgacg ccggccatgc
     2041 ggggcatcga ctgctgcagg ccgccgaagt cctcgatcct gcgggtggcc cggcgctcct
     2101 cgagcatgcc ggtcacgaag aagaggccgc cgatgacgat cccgtgggcc accatgtaga
     2161 ataccccgcc ctgcaggccc tggacggtga aggcgaagat gccgagcacg atgaagccga
     2221 ggtgggacac tgacgagtag gccaccagac gcttgaagtc cttctgcacg gcggcgatga
     2281 ccgcgcagta gaggatgccg accacggcca gggtcatgag cgccggctgg aagtaccggg
     2341 cggcgtccgg gaacaggggc agggcgaagc gcaggaagcc gtagatgccc agcttggaca
     2401 tgaccccggc gagcaggacg gtggtcgtcg tcggggcctc ggcgtaggcg tcgggcagcc
     2461 aggtgtggaa ggggaccagg ggcgtcttga ccgcgaaggc ggcgaagaag gcgccgaaca
     2521 gccagcgctg ggtggtcggg tccttggaca ggtcgcgcag ggcctcgtag tcgaacgtgc
     2581 ccgggccggc ggccctggac ttcaggtagt agaggctgac gatccccacc agcatgagca
     2641 ggccgccgaa cagcgtgtag atgaagaact tgacggccgc atacacccgc cgctcgccgc
     2701 cccacgaccc gatgaggaag taggcgggga tcagcacggc ctcccagaag atgtagaaca
     2761 ggaacatgtc gagggccgcg aagaccccga gcatggccgt ctccaggacc agcagcaggg
     2821 cgaggtagtt gcgcggccgg tcggtcatgc gccacgagcc ggcgatcgcg agcggcacca
     2881 gcacggtggt gagcatcacg agcggcaggc tgaccccgtc gaccccgacc ttgtactgga
     2941 cgccgaacga gcgcacccag ttggcgtgct gcccgagctg gaagccggcc tcgcccgtgc
     3001 ggaaccgggc ggcgagggcg accgcgaggc cgagggtcac caggctcgcg agcagggcga
     3061 cccagcgcac ggcccggtcc agcggggcgg ccgtggcggg ctctcccggc ccccccgagg
     3121 ccctggccct cgggatggcg gcgaccagca gggcccctgc cagcggggcg aacacggtga
     3181 gggtcagcag cacggcgggg ctcctaggtg gtccggaaga cgaggaaggc gatgatcagc
     3241 accgcgccgc cgagcacgcc aaccgcgtag gtgcgcacga ggcccgactg gagccggcgg
     3301 ccgcggccgg cgacggcgcc gaccgcctcg gccgcgccgt tgacggcgcc gtcgatggcc
     3361 cgctggtcca cgttcacggc caggaaggcg gccagcgccc ggccgagcgt gacggtgaag
     3421 aactcgtaga cgtcgtcgac gtagagcttg ttggcgagca ggcgccatgc gggcgcgtag
     3481 cggacccgca gcgcggccca ctcgaccctg cccgagaggt agacccacca ggcggcgagc
     3541 gccccgaccg cggcggcgcc gaccgcggcc aggctgagca ggaagcccgc gaggaacgac
     3601 ctcgactcct cgggcgcccg cccgagcacg ggctcgagga agcgcccgat cgcgccgacg
     3661 cccgagaagc cgccgaggac gcccccgacc agtgcgagga ccgcgaggac gagcagcggg
     3721 acggtcatga cggccggcgc ctcgtggggg tggcggccgc tcggccagcg cggctcgccg
     3781 aagaaggtga ggaagtagag ccgggacatg tagaaggcgg tgagcccggc ggtgagcacg
     3841 ccgaccaccc aggcgaacag gccggcgccc ccgtgcgcga acgcggcgcc gaggatcgcc
     3901 tccttggaga agaacccgga ggtgagcggg aagccgatga tcgcgagcca ggccgccccc
     3961 atggtgagga acgtgaccgg catggccctg cgcaggccgc cgaaccgctt gacgtcggtg
     4021 tcgtcgtcgt tgccgtgcat caccgacccg gcgccgagga acagctgcgc cttgaagaag
     4081 gcgtgggtga cgaggtggaa caggccggcg gcgtagccga tggggccgag cccgaccccg
     4141 atgaacatgt agccgagctg ggacacggtc gagtaggcga ggatccgctt gatgtcgtcc
     4201 tgcccgacgg cgatgagcgc ggcgaccagg gcggtcaggg cgccgatgac cacgaccacg
     4261 gtctgcgcgg tgccggaggc ctcgaacagg acgtgcgtcc gggccacgag gaagacgccg
     4321 gccgtcacca tggtcgcggc gtggatcagg gcggacaccg gcgtggggcc ggccatggcg
     4381 tccggcagcc agacgaagag ggggatctgg gcgctcttgc cggtgcaggc gaggaacagc
     4441 agcagcgcga tcgcggtggc cgtcccgccc ctgaggctcc caaccctgcc gaggacgtcg
     4501 gagaagtcga gactgccgaa cttctggaag atcaggaaca tggcgatcag gaagcccacg
     4561 tcgccgatgc ggttggccac gaacgccttc ttggccgcgg ccggcacccc ttcgcggttg
     4621 aaccagaacc cgatgagcag gtacgagcag agcccgaccc cctcccagcc gatgaacatg
     4681 accaggaagt tctgggcgag gaccagcacg agcatcgcgg ccacgaacag gttcagccag
     4741 gagaagaagg tctcgcgtcg cccgtcgtcg gccatgtagc cgatcgagta gaggtggatg
     4801 aggctcgaca ccccggtgac gaccagcgcc atggtggccg agagctggtc ccagcggagg
     4861 tcgaaggcga cccgcaggct gcccgaggtg atccacccgt agccgtgcac cacctgggtg
     4921 cgctcgtccc cgctgtggga gagcagcgcg aggaaggcgg ccacggcgat cgcgaacgag
     4981 gccgcgacca gggcggtggc gagccatccg gcgagcgcgc ggagccgctg cccgagcgcg
     5041 gcgttgaccg cgaacccggc cagggggagg acgggcagca gccagacgag tcggatcgtg
     5101 ccggtcgcgt tcagggcagg gtcacccctt gagcaggttg acgtcgtcga cgctgatcga
     5161 cgagcgcttg cggaagatgg cgacgaggat gccgaggccg acgacgacct cggcggccgc
     5221 gaccaccatc acgaagaacg ccaggacctg gccgtcgagc gtgccgttgc gcctggcgaa
     5281 ggcgacgagc gacaggttga cggcgttgag catgagctcg acgcacatga acaccacgat
     5341 ggcgttgcgg cggatcagca ccccgaccac cccgatggtg aacagcagcg cgctgagcag
     5401 caggtaggcg ttggtcccga ccttcatggg cgacgcttcc cgaggacgag ggccccgacc
     5461 gcggccacga cgagcaggat cccggtcgcc tcgaaggcga acacgtaccg ggtgaacagc
     5521 aggcggccga ccgcctgggt gttgccgctc tggttggccg ccgcgaggtc accggccggg
     5581 ccggacaggc ccccgttgag gacggcgacg acgaccgcga cgagcaggag cacgccgagc
     5641 acgccggcga tcgtccgctg gccggggatg gtctccctga ggacgtcctc gttgccgatc
     5701 ccgagcagca tgagcacgaa caggaacagc accatgaccg cgccggcgta gacgatcacc
     5761 tggaccgcgg ccaggaactg ggcctgcagc aggacgtaga agccggccag gcagaagaag
     5821 ttcacgacca gcagcagggc cgagtagacc gcgttgcggc tgaggaccat gccgaccgcg
     5881 gcgacgatgc tgagcagcgc gagcgggacg aaggtgacca gctcggcccg gtcggcggcg
     5941 gtggtggcgg cgacggcgat catccgggga cgacctcgtc ggtcgggagg tcgcggtcgg
     6001 cctgggtcgt ctcccgggcg cgcagcagcg cccagaccgg ctcggacgac tcctgggaga
     6061 tcgacgcctg cggcccgtag cgcgcgatcg cgggcggccc gtccccggcg tcggcgtgcg
     6121 gcggcggctc ggcgccggcc gggaccgggg cgaggagccg gggcttgtcg tagacgagct
     6181 cctcgcggct gtcggccgac atctcgtact cgttggtcat cgtcagcgcc cgcgtggggc
     6241 acgcctcgat gcacaggccg cagaagatgc agcggttgta gttgatctgg tagatggcgg
     6301 cgtagcgctc gccgaccgag tagcgcgcct cgggggtgtt gtcggccccc tgcacgtaga
     6361 tggcgtcggc cgggcaggca tacgcgcaca gctcgcagcc gatgcagcgc tcgaggccgt
     6421 cgtcgtaccg gttgagctgg tggcggccgt ggaagcgcgg cgaggtgggg cgcttcacct
     6481 ccgggtactg gatggtgttc ttcttgcgga acatgctcct gaaggtgacc gagaggcctt
     6541 tcaggatctc cgagggcatc atggggtgga ccctcccggg gcggtgcggt ccggggcgcc
     6601 aggcttgggc ctggactcct tggggctggg gaccaggaag gacacgagca gcaggaccag
     6661 caggatcccg atcccgatga agagcacccg gcggcccggg ctcgacaggt tcacggtgag
     6721 cacgacagcg gtcaccgcga tccaggcgag ggccaccggg atgaggaact tccagccgat
     6781 ggccatcagc cggtcgaagc ggagccgcgg cagcgtggcc cgcagccaga tgaacacgaa
     6841 gatgaacagg tagatcttga ccacgaacca caggatcggc cagagccacg gcaggaagcc
     6901 gtggatggtc gggccgtccg gcccgcccag gaacagggtg gtcgcgaccg ccgagacggt
     6961 gatgacgttc aggtactcgg ccaggaagaa cagagcgaac ttcacgcccg agtactcggt
     7021 gtggtagccg ccgaccagct ccgtttcggc ctcgggaagg tcgaacggcg cccggttggt
     7081 ctccgcgatc ccggcgagca ggaagacgaa gaacgcgggg agctgcggga cgatgtacca
     7141 gcgggggaag acgccccaga tggggttgcc gtgctgggcg gcgacgatcc cggacgccga
     7201 cagggtgccc gcgtacatga gcaccccgac gatcgacagg ccctgcgcga tctcgtagct
     7261 gatcatctgg gcgctcgagc ggacggcgcc gagcagcggg tagttcgacc cggaggccca
     7321 cccggcgagc acgatcccgt acacctgcag gctgcccatg gcgag
//
LOCUS       DASPYY010000468         5131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1344009, whole genome shotgun sequence.
ACCESSION   DASPYY010000468 DASPYY010000000
VERSION     DASPYY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344009"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..627
                     /locus_tag="VEP73_07345"
     CDS             199..627
                     /locus_tag="VEP73_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HYT26279.1"
                     /translation="MATKPWHLPLRVATGAFILNSGLSKLDADEERAKQLHAMASTAF
                     PQFADMDPKQFATLLSTGELLLGTALLAVPFVPPLVAGLGLVAFATGLNRLYLKVPGM
                     RQEDSVKPTQQGIPLAKDVWMTAIGAALVLDSIFAPRRRR"
     gene            776..1668
                     /locus_tag="VEP73_07350"
                     /pseudo
     CDS             776..1668
                     /locus_tag="VEP73_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
     gene            complement(2716..2789)
                     /locus_tag="VEP73_07355"
     tRNA            complement(2716..2789)
                     /locus_tag="VEP73_07355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2755..2757),aa:Gly,seq:ccc)
     gene            2896..3465
                     /gene="dcd"
                     /locus_tag="VEP73_07360"
     CDS             2896..3465
                     /gene="dcd"
                     /locus_tag="VEP73_07360"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HYT26280.1"
                     /translation="MILSDRSLREAIAAGRLVVDPLEDQSIQPSSIDVRLDSRFRVFH
                     NARYPYIDVREPMDGLTELVEVKPDDAFILHPGEFVLGSTLELVGIADDLAARLEGKS
                     SLGRLGLLTHSTAGFLDPGFQGHVTLELSNVANLPITLYPGMRIGQIAVFQLTTPAER
                     PYGARGVNSKYQGQRGPTPSRYWENFRRP"
     gene            complement(3472..>5131)
                     /locus_tag="VEP73_07365"
     CDS             complement(3472..>5131)
                     /locus_tag="VEP73_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYT26281.1"
                     /translation="GSHTGQAWVILAMIAGVVVTLLVYRGARAARGTLPYGGWAFASH
                     AVGRLLDGLPPNALRAVDETSLLAHLAVVLSFLVLVMYSKHLHIFSAPFNVAFSRRPK
                     ALGPLSTPVIDVETMSEDDVFGVGRVEDFKWKQLLDLATCTECGRCQDQCPAWNTGKP
                     LSPKLVITDLRDHLFAKGPFLLGDASPEAPDGHDPALLEQDLVKDVVGYDVLWSCTTC
                     GACVEQCPVDIEHVDTILDMRRYEVLMESRFPAEAGTMLRNVENAGDPWGLGSGQRLS
                     WTEGLEFEVPVVTGKIPDGVEYLYWVGCAGALEDRAKRTTRSVARLLHRAGVSFAVLG
                     PRESCTGDPARRLGNEYLYQMQAQRNIETLNQAGVRKVVASCPHCFNSIAREYPDLGG
                     RYEVVHHSQLLGRLVEEGRLAPSARVDATVTYHDPCYLGRHNDVYDAPRAVLDAVPGV
                     RSVEMHRCRERGFCCGAGGARMWMEERIGKRVNLERTDEALATGADVVGTACPYCMVM
                     LDDAIKQRQAEGAADGVRVLDVAQVLEESVATEPAAGSSRRAAE"
BASE COUNT          700 a         1919 c         1846 g          666 t
ORIGIN      
        1 gtggacgcag ctgcacggcg gtgggctgac ccggcacggc gggctccagg gtcggtggca
       61 cgacgcgggg cgagggctca gcctttcacg accgggccgg cgccgccacc agccgtaccg
      121 ggagggatac cggcggggag gcggggtaat ctcgttgctc ggacaaccat tcggacaacc
      181 atgaggagga gccgaaccat ggccaccaag ccttggcacc tgcccctgcg ggtcgcaacc
      241 ggtgccttca tcctgaactc cgggctctcc aagctggacg ccgacgaaga gcgcgccaag
      301 cagctgcacg ccatggcgtc gacggcgttc ccgcagttcg ccgacatgga ccccaagcag
      361 ttcgccacgc tgctgtcgac cggtgagctc cttctcggga cggccctgct cgcggtgccg
      421 ttcgtccccc cgctcgtggc cggcctcggc ctcgtcgcct tcgcgaccgg cctgaaccgg
      481 ctctacctga aggtgcccgg gatgcgccag gaggacagcg tcaagccgac ccagcagggg
      541 atccccctcg ccaaggacgt gtggatgacc gccatcggcg ccgcactggt gctcgactcc
      601 atcttcgcgc cgcggcgccg gcgctgaccg cccgccaggc acggcccctg gtccgtccgc
      661 gccggtcgct caccggcccc cggggtgcgg ggtcaggcat ggccgaggga acccggccgg
      721 cgtgcccgcc gtctcactga cgtcggcgtc ggtgggacgg cagcgggagg acaggatgcg
      781 ggcgagcagg tggggagctg ggctggcgct ggcggcggtg ctggccgcgg gctgcagcgg
      841 agcgacggga ggctcgacga gcgcggggag cgcgggtggc ggcgcggggg ccgccaggca
      901 ggcggcgccg gcggccacgc ccaaggcggc cgggggcgcg gcgggaagcg tccggctggt
      961 cgacctgggc aaccggatcg tccgcaccgc caccgtcgac ctccaggtcg ccaagggcgg
     1021 cctcggcgac gccatcacgc gagccaccga cgtcgtggcc aggtacaagg ggatgtacgt
     1081 cggttcgacg acctcgatcc cgagcgacag cccacctcgg ggccaggtca ccttccgggt
     1141 gccggtcgag gcgttcgagg ccaccctgcg cgagctcaag gggctcggca cctaccgggg
     1201 cgagcggtcg agcaccgagg acgtcacaac cagtacatcg acctgaaggg ccagctcgcc
     1261 gcctggcggg cccaggagca ggcctacctg aagctgctcg accgggccag gtcgatctcc
     1321 gacgtcatcg cggtgcagaa ccagctccag caggtgcagt cgaacgtcga gcgcctccag
     1381 gggcaggtga actacctgga ggaccagtcg tcgttctcga ccatcgtgct cgacctcagg
     1441 gagcccgggg cgggcccggc cgccgcgaag ccccgcggca cgttcgccag ggcgtggcac
     1501 accgcgctcg ccggcctcac ggccatggcc gcggcggctc tggtggccgt ggtctggctc
     1561 gcgccgctcg ccctgctcgc ggtcgcggtg ctcctcgtgt tgcggctggc gcgccggccc
     1621 cgccaggcag gcccggcggc gacggagccc ggctcctcgg ccggctgacc gcccctgacg
     1681 ggatcctccg tgcggcagcc ggcgctcaag gcggggagca gacctgccga tgagcaacgc
     1741 gtacggaccg cagcaaccga agcgccgaac tcccccgaga acctaccgcc gtaggggaac
     1801 ccccgtcgtg ctcccacgcc gcagcgcacc ggtcgtgctc gggctggccg tcacctccct
     1861 ggtgacgatg ctcctcgccg gccagctcgt gatcgagacg accagcctct cctttgactc
     1921 cacccgtgac tccacccggc cggtcgagct caccctcggg tcgctcggcc cggtcgaggc
     1981 cgatgccccg gccggcgcgg caacgaggac cgcaccgggt ccgccggggc ggtcccgagc
     2041 cgctggcggg accgccgggg ccgggtccgc cgcgccaccg gcaacgggcg gccgggcgcg
     2101 gcgccttccg ccgccgccca ggggcttcga cgccacgccg aacgtggtcg cggtcgtcga
     2161 ccggcacgcg gaccgtgccg ggaagcgggt cgcgcgcgct cccgagcctc cgaaggaccg
     2221 catcccgatc gtcatcggcc atcgtgggct gccctgctcc gagcgtcggc gctggaccgg
     2281 acgtccgccc ggctcctgcc cccggccgaa gccctctccc ggcccgccct cacggcgccg
     2341 gtgcccgccc gcgccgagcc ccagccccga gccgccgctg ctcgcgctgc tgccgcacgg
     2401 cccgccagcc atggccacgg ccgggagcga accgccccgc ccggtcggcc cgcggcggcc
     2461 cccgagccgc tcgtcctccg cgggggccgc cgtccgcagg tgccggcgaa ttcgcgttcg
     2521 gtagatgcac ggcgtctatg cggggcgacg ggcgacgacg tgcactgggc ggtggccggc
     2581 atccacgccg acacgacgag cgtccccgag ggcacggtgc cggcgggcgc ggtggtggtc
     2641 cgcgagtacc tcgggccctg cccgcccagc ggcacccaca cccgtgccga ctggtgcgtc
     2701 gccgggcacg gcgggtggag cgggcgaagg gaatcgaacc ctcactgcga gcttgggaag
     2761 ctcgtgttct accattgaac tacgcccgcg cgggccgcga ttctagccgc catcgcggag
     2821 ctgcggcaac ggggctggct tcgggcggca tgcgagaatg cgcgcatctg caccccgacc
     2881 cgttggagcc gcgtcgtgat cctgtccgac cgcagcctgc gcgaggcgat cgccgccggg
     2941 cggctggtcg tcgacccgct cgaggaccag tccatccagc cctcgagcat cgacgtgcgg
     3001 ctggacagcc gcttccgcgt cttccacaac gcccgctacc cctacatcga cgtcagggag
     3061 ccgatggacg ggctcaccga gctcgtcgag gtcaagccgg acgacgcctt catcctgcac
     3121 ccgggcgagt tcgtgctcgg ctcgaccctc gagctcgtcg gcatcgccga cgacctggcc
     3181 gcccgactgg aaggcaagtc gagcctgggc cggctcggtc tgctcaccca ctcgacggcc
     3241 gggttcctgg atcccggctt ccaggggcac gtgaccctcg agctgtccaa cgtggccaac
     3301 ctgccgatca ccctctaccc ggggatgcgg atcgggcaga tcgcggtctt ccagctcacc
     3361 acccccgccg agcgccccta cggcgcgcgc ggtgtgaact cgaagtacca gggccagcgc
     3421 ggaccgaccc cgtcccgcta ctgggagaac ttccgcaggc cgtagccgcc gtcactcggc
     3481 ggcgcgccgg gacgacccgg ccgccggctc ggtggccaca gactcctcga ggacctgggc
     3541 cacgtcgagg acccgcaccc cgtcggccgc cccctccgcc tggcgctgct tgatcgcgtc
     3601 gtcgagcatc accatgcagt acgggcaggc ggtgcccacc acgtcggcac cggtggcgag
     3661 cgcctcgtcg gtccgctcca ggttgacccg cttgccgatg cgctcctcca tccacatgcg
     3721 cgccccgccc gccccgcagc agaagccgcg ctcgcggcag cggtgcatct ccaccgaccg
     3781 gacgccgggc accgcgtcga gcaccgcccg cggtgcgtcg tagacgtcgt tgtggcggcc
     3841 gaggtagcag gggtcgtggt acgtcacggt cgcgtcgacc cgggccgatg gcgcgagccg
     3901 tccctcctcg acgagccggc cgagcagctg ggaatgatgg accacctcgt agcggccgcc
     3961 gaggtccggg tactcgcggg cgatcgagtt gaagcagtgc gggcaggagg cgaccacctt
     4021 ccgcaccccg gcctggttca gcgtctcgat gttccgctgt gcctgcatct ggtagaggta
     4081 ctcgttgccg agccggcggg ccgggtcgcc ggtgcaggac tcgcgggggc cgagcaccgc
     4141 gaacgagacc ccggcccggt gcagcaggcg ggcgaccgac ctggtggtgc gctttgcccg
     4201 gtcctcgagc gcgccggcgc agccgaccca gtagaggtac tcgacgccgt ccgggatctt
     4261 cccggtcacc accggcacct cgaactcgag gccctcggtc caggacaggc gctggccgct
     4321 tccgagcccc cacgggtcgc cggcgttctc gacgttgcgg agcatcgtgc ccgcctcggc
     4381 cgggaagcgg gactccatga gcacctcgta gcggcgcatg tcgaggatcg tgtcgacgtg
     4441 ctcgatgtcc accgggcact gctcgacgca ggcgccgcag gtggtgcacg accacagcac
     4501 gtcgtagccg accacgtcct tcacgaggtc ctgctcgagc agggcggggt catggccgtc
     4561 cggcgcctcg gggctcgcgt cgccgagcag gaacggcccc ttggcgaaca ggtggtcccg
     4621 caggtcggtg atgaccagct ttggcgagag cggcttgccg gtgttccacg ccgggcactg
     4681 gtcctggcag cggccgcact cggtgcaggt cgcgaggtcg aggagctgct tccacttgaa
     4741 gtcctcgacg cggccgacgc cgaagacgtc gtcctcgctc atggtctcga cgtcgatcac
     4801 cggggtcgac agcggcccga gcgccttggg gcggcgcgag aacgccacgt tgaagggcgc
     4861 gctgaagatg tgcaggtgct tggagtacat cacgagcacg aggaagctca gcacgacggc
     4921 gaggtgggcg agcagggacg tctcgtcgac ggcgcgcagg gcgttgggcg ggagcccgtc
     4981 gaggagccgg ccgacggcgt gcgaggcgaa cgcccatccg ccgtagggca gggtgcccct
     5041 cgccgccctc gccccgcggt agacgagcag ggtgacgacg acgccggcga tcatggccag
     5101 gatcacccac gcctggccgg tgtgcgaccc g
//
LOCUS       DASPYY010000469         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1062761, whole genome shotgun sequence.
ACCESSION   DASPYY010000469 DASPYY010000000
VERSION     DASPYY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062761"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VEP73_07370"
     CDS             complement(<1..958)
                     /locus_tag="VEP73_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYT26282.1"
                     /translation="MSLRGRLLAATLLLVAAGLAAADLATWAALRSFLLDRVDQQLDA
                     TRRQVVPAVRARGPGAVYQVRRRGAVLPDSSSTTYVEVRDAGNQVLGATSGGAVPDLP
                     QTLPVSLPPSGTGRVRVATFVVASRGRGAPYRVQAAAVPSDGFPFPGGGAGFFVAVAV
                     PLGEVLQTLGRLVAIEALVSLAVLVGVSVLAARMVRVGLRPLDEIAATAAAIAAGDLG
                     RRVGRAEPRTEVGRLGLALNRMLGQIEGAFVQRTVSEARLRRFVADASHELRTPLTSI
                     RGYAELFRRGAGTRPADLATTMRRIEDEAGRMGVLVDELLLLA"
     gene            complement(961..1653)
                     /locus_tag="VEP73_07375"
     CDS             complement(961..1653)
                     /locus_tag="VEP73_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26283.1"
                     /translation="MLVGAEGRILVVDDEVYITDLVSMALRYEGFEVEAARTGREALA
                     TAESFRPDLVVLDIMLPDMEGLEVCRRLRDGEGMPVVFLTARDATEDKVTGLTVGGDD
                     YVTKPFSLEELVARIRAVLRRTRPAAESGRLAFADLELDEDTYEVWRQGTPIQLTSTE
                     FKLLRYLVRNARRVVSKAQILDAVWQYDFGGNDNIVETYVSYLRRKVDRFDPPLIHTV
                     RRVGYSLRLPTA"
     gene            2001..2486
                     /locus_tag="VEP73_07380"
     CDS             2001..2486
                     /locus_tag="VEP73_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26284.1"
                     /translation="MSMQSWREPSDRWEREPEAWGPGEASGSGASQAPGWEADVWQAQ
                     EWDDDACLEDEPGEPNGGVVAAIVSAGIACAILGSVVVAAAASPAFAKLMNFYSPAGP
                     ITGKTTVTTVAYLVIWPNLHYRLRDRHLDLSKAFLFTIFLVAVGLVGTFPPFYQLFAP
                     R"
     gene            complement(2462..3187)
                     /locus_tag="VEP73_07385"
     CDS             complement(2462..3187)
                     /locus_tag="VEP73_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT26285.1"
                     /translation="MPSPASCWCTAATASSRRPVPSSATTRSASSRAAACTSCSTPGS
                     RAPPPRPSRSNGSQLPTRTLVWAAGNRPAPLLARLPFERGTTGAVLVDGSLQVRGAGN
                     VWALGDCARVPDPDRGGRACPPTAQHALRQGRTAAENIVRALEGRRPRPFAFRALGFL
                     VLLGHGAAAADIRGVRFSGLLAWLMWRGVYLSKLPRREKRLRVLLDWTIELFFPRDIA
                     LTVNDASPPISRPVPTYRGAKSW"
     gene            3520..>4187
                     /locus_tag="VEP73_07390"
     CDS             3520..>4187
                     /locus_tag="VEP73_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26286.1"
                     /translation="MATSTSSTTASRNRVRGQADRTGTLMWFGSSELDATSASIGVNS
                     IALLWLTTVTSMRGIRGRSRSSWRAVLTPAKPPPRMTTRYGGARPWPSTDQASGSMYL
                     VAASWNRSPASEPSRRTPRRLGSCAPASAPDHATAPLTRRGMTVRDMTPHRTMPMSRP
                     PVSARAWLRGRCRNAAAASQERPVSAAARRPRRLPWPARAVSVARRAGRACRPVRTPP
                     SAVPR"
BASE COUNT          580 a         1528 c         1586 g          493 t
ORIGIN      
        1 gggcgagcag gagcaactcg tcgacgagca cgcccatgcg cccggcctcg tcctcgatcc
       61 ggcgcatcgt ggtggccagg tcggccgggc gcgtgccggc gccccgccgg aacagctcgg
      121 cgtagccccg gatcgaggtg aggggcgtgc gcagctcgtg ggaggcgtcg gcgacgaacc
      181 ggcgcagcct cgcctcggag accgtccgct ggacgaacgc tccctcgatc tggccgagca
      241 tccggttcag cgcgaggccg aggcggccca cctccgtgcg gggctcggcc cggccgaccc
      301 gccgcccgag gtcaccggcg gcgatcgccg ccgccgtcgc ggcgatctcg tcgagcggcc
      361 ggaggccgac gcgcaccatg cgcgccgcga ggacgctcac gccgacgagc accgcgaggg
      421 agaccagagc ctcgatagcg accagacgcc cgagcgtctg gagcacctcg cccagcggga
      481 ccgcgacagc gacgaagaac cccgcgccac cacctgggaa cgggaacccg tcgctcggga
      541 cggcggcggc ctggacacgg tagggggcac cacggccccg gctggccacg acgaaggtgg
      601 cgacccgcac gcgtccggtg cccgacgggg ggagggagac cggcagggtc tgcggcaggt
      661 caggcaccgc gccgcctgag gtcgcgccga gcacctggtt gccggcgtcc cggacctcga
      721 cgtaggtcgt ggacgaggag tcgggcagga ccgcgcccct gcgccggacc tggtagacgg
      781 cgcccggtcc gcgggcgcgc acggcgggca cgacctgccg ccgggtcgcg tcgagctgct
      841 ggtcgacccg gtcgagcagg aaggagcgga gggccgccca ggtggccagg tcggcggcgg
      901 cgagaccggc ggcgacgagc aggagcgtcg cggccagcag cctgccacgc agcgacatgg
      961 tcacgccgtc gggagccgca ggctgtagcc gacccgccgg acggtgtgga tgagcggagg
     1021 gtcgaaccgg tcgaccttgc ggcggaggta gctcacgtag gtctcgacga tgttgtcgtt
     1081 gccgccgaag tcgtactgcc agaccgcgtc gaggatctgt gccttcgaca cgacccggcg
     1141 cgcgttgcgg acgaggtagc ggagcagctt gaactcggtg gaggtgagct ggatcggcgt
     1201 cccctgccgc cagacctcgt aggtgtcctc gtccagctcg aggtcggcga acgcgaggcg
     1261 tccggactcg gcggccggtc gggtgcgccg caggacggcc cggatgcggg cgaccagctc
     1321 ctccaggctg aacggcttgg tgacgtagtc gtcgccgccg accgtcagcc ccgtcacctt
     1381 gtcctcggtg gcgtccctgg cggtgaggaa gaccaccggc atgccctcgc cgtcccggag
     1441 gcggcggcag acctcgaggc cctccatgtc cggcagcatg atgtcgagga cgaccaggtc
     1501 gggccggaag ctctcggcgg ttgcgagcgc ctcgcggccg gtccgggccg cctcgacctc
     1561 gaacccttcg taacggagcg ccatgctgac gaggtcggtg atgtacacct cgtcgtccac
     1621 gaccaggatc cggccctcgg ctcccaccag cacctccctc gccgggcgcc gcgcgcgccg
     1681 gcctctcgca gtatgcccgc cgttcctggg agccggctgt gcgtcggctg agcgcgcgca
     1741 gggaagccgc cggggccggc gcgggccgct cgtgagcttg cccgatcggc cccgcgttgc
     1801 tagcctcacc ccgacccgca gcgccctcgg gtgcccccac acccgaatcc acccccagga
     1861 agggcgcttt cgtgtacgag gtcatctgct ggccagcgcc cgcgtgcccg ccggtccacc
     1921 gggcgttcgt cccccgagga gcacggccaa ggagcacagc cgaggagcac agccaaggag
     1981 cacagccaag gagcacggcg atgagcatgc agagctggcg tgagccatcc gaccgctggg
     2041 agcgggagcc cgaggcatgg ggacccggcg aggcgagcgg ctccggcgcc tcccaggcgc
     2101 cggggtggga ggcggacgtg tggcaggcgc aggagtggga cgacgacgcc tgtctcgagg
     2161 acgagccagg cgagccgaac ggcggcgtcg tcgcggccat cgtgtccgcc ggcatcgcgt
     2221 gcgcgatcct cgggtccgtc gtggtcgcgg ccgccgcgag cccggccttc gccaagctca
     2281 tgaacttcta ctcgcccgcc ggaccgatca ccgggaagac gacggtcacc accgtcgcct
     2341 atctcgtgat ctggccgaac ctgcactacc ggctgcgcga ccggcacctc gacctctcca
     2401 aggcattcct cttcacgatc ttcctcgtgg ccgtcgggct cgtcggcacc ttcccgccct
     2461 tctaccagct cttcgcgccc cggtaggtgg ggaccgggcg tgagatgggc ggcgaggcgt
     2521 cgttcacggt gagcgcgatg tcgcgcggga agaacagctc gatcgtccag tcgagcagca
     2581 cccggagccg cttctcgcgg cggggcagct tgctgaggta gaccccgcgc cacatgagcc
     2641 aggcgagcag cccggagaac cgaacccccc ggatgtcggc cgccgccgcg ccatggccga
     2701 gcaggaccag gaacccgagc gccctgaagg cgaacggccg cgggcgcctg ccctcgagcg
     2761 cccgcacgat gttctcggcc gccgtcctgc cctgccggag cgcgtgctgc gccgtcggag
     2821 ggcaggccct gccgccgcgg tccgggtcgg gtacccgggc gcagtcccct agcgcccaga
     2881 cgttgccggc gccgcgcacc tgcaggctgc cgtccacgag cacggcgccg gtcgtgccgc
     2941 gctcgaacgg cagcctggcg agcagcggcg cggggcggtt gcctgccgcc cagacgagcg
     3001 tgcgggtggg gagctggctg ccgttcgagc gcgacggcct cggcggtggc gctcgtgacc
     3061 cgggtgttga gcaggaagtg cacgccgcgg ccctcgagct tgcggagcgc gtagtcgccg
     3121 agctcgggac cggtctccgg gaggatgcgg tcgcggccgt gcaccagcac gaagcggggc
     3181 tcggcagcgg tgatggacgg gaagaagcgc cggacgccgt gcaccaggtc gcagagcccg
     3241 gccatcatct cgacgccggc gaacccgccc ccggcgacca cgaaggtgag ctggcggcgg
     3301 cgctcggcgg gggtggtctc cacgtcagcc tgctcgagcc gggcgatcac gtggttgcgg
     3361 agcctggtcg cgtcggcgag ggtcttgagc ggcagggagc tcgtctgcac cccggggagg
     3421 ttcaggtagt ccggcacggc gccgagggcc aggaccaggt ggtcgtaggg cagcgccccg
     3481 gccggccggg ggccgctcgc acggaggcgc acgagccgct tggcgacgtc gacgtcctcg
     3541 accacggcga gccggaaccg ggtccgcggg caggccgacc gcaccgggac gctgatgtgg
     3601 ttcggctcga gcgagctgga cgcgacctcg gccagcatcg gggtgaacag catcgcgttg
     3661 ctctggctca ccaccgtcac ctccatgcgc ggcatccgcg ggaggagccg ctcgagctgg
     3721 cgggccgtgc tcacgccggc gaagcccccg ccgaggatga ccacccggta cgggggcgcc
     3781 aggccgtggc cgtcgaccga ccaggcgtcc gggtccatgt acctggtcgc cgcctcgtgg
     3841 aacaggagcc cggcgagcga gccgagcagg aggacgccga ggaggctcgg gagctgcgcc
     3901 ccggcgtcag cgcccgacca cgcgaccgcc ccgctcacca ggaggggcat gaccgtgagg
     3961 gacatgacgc cccacaggac gatgcccatg agcaggccgc cggtgagcgc ccgggcgtgg
     4021 ctgcggggcc ggtgccggaa cgcggccgcg gcgagccagg agcggcccgt gagcgccgct
     4081 gcccggcgtc cccgccgcct cccctggccg gcccgcgccg tcagcgtcgc gcgccgcgcc
     4141 ggccgcgcgt gccgtcccgt gcgcacgccg ccctcggccg tcccacg
//
LOCUS       DASPYY010000470         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_781583, whole genome shotgun sequence.
ACCESSION   DASPYY010000470 DASPYY010000000
VERSION     DASPYY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781583"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VEP73_07395"
     CDS             complement(<1..258)
                     /locus_tag="VEP73_07395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT26287.1"
                     /translation="MHLVEIVWRCSPRSYSCWHVAHVGVRPGMDVLDVACGHGRIANR
                     LAEHGCPMTGLDATPAFLDRARRDAAPGMWSWTVSRATWTLA"
     gene            330..835
                     /locus_tag="VEP73_07400"
                     /pseudo
     CDS             330..835
                     /locus_tag="VEP73_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835118.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            832..1230
                     /locus_tag="VEP73_07405"
     CDS             832..1230
                     /locus_tag="VEP73_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYT26288.1"
                     /translation="MRPLDPLSSIKVKLGVVILAAVAVTIVVEYVGLLLGVNPWLSTV
                     GAAVLALAMVQFLAHGMTSPLREMAAAARAMARGDYKRRVTASSRDEVGELARAFNAM
                     AAVLAEIEPPDPETLRTMLRRSGRPSASAA"
     gene            1230..1910
                     /locus_tag="VEP73_07410"
     CDS             1230..1910
                     /locus_tag="VEP73_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HYT26289.1"
                     /translation="MVQLLAGLCALFAAFTAAQLVALSEGGRHVLATAGLTYAEYARS
                     GLFQLLAVAVITLGVLLLLRAATDLASPGRRATFTAVTELAVALTLVVVVVAVRRLNL
                     STSPTRRPSSPATSSPASVRSSAPMSSTSPASLRTPSPPWPPACPAWTRSREPTCAPG
                     SAPSSPTASRRPASPAGPLGTLATSAPSGPSPTSAADKQRITPLASPGPRRADHGLVV
                     AGLGAVPS"
     gene            2009..2980
                     /locus_tag="VEP73_07415"
     CDS             2009..2980
                     /locus_tag="VEP73_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYT26290.1"
                     /translation="MAELTRDEALSIYDRMVTIRAFEERVAKLFADGRIPGFVHLYAG
                     EEAVAVGLCAHLEDRDFITSTHRGHGHCIAKGVELGPMMAELFGRVDGSCKGKGGSMH
                     IADVSRGMLGANGIVGGGPPLACGAALTAKHKGTGAVCVCFFGDGAAEQGTTHEAMNL
                     AAIWKLPVVFVAENNGFAESTPVGYHCAASTIADRAASYDIPGISVDGYDVVAVYEAA
                     EEAVSRARRGDGPSLIEAKTWRYFGHFEGDQVTYRTAEQSAAYREHDPLTYFARQAVD
                     RDLLTADQVEERNRAAEAAVDEAIAFAERSPEPAPEDALTDVYVTYR"
     gene            2995..4026
                     /locus_tag="VEP73_07420"
     CDS             2995..4026
                     /locus_tag="VEP73_07420"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYT26291.1"
                     /translation="MSVTTSSVARTATFREAVNEALRLEMERDPDVVLMGEDVAGGAT
                     IEGFEREDAWGGVLGVTKGLVQRFGRERVLDTPISESGFIGAAVGAAATGLRPVAELM
                     FVDFFGVCMDQIFNQGAKLRYMFGGKARVPVVIRTMIGAGFRAAGQHSGCHYSVFTHM
                     PGLKTVVPSTPADAKGLLAASIRDDDPVLFFEHKLLYDTTGEVPEGEYLVELGRADVK
                     REGDAATVVAIGRMVNLALEAAGTLSASGVEVEVVDPRTLSPLDDEAILASVRKTGRV
                     VVVDEDNPRCSMATDIAALVASRAFDHLDAPPQLVTAPHTPVPFSPVLEDFYVPTPER
                     IAEAVQATL"
     gene            4039..>4543
                     /locus_tag="VEP73_07425"
     CDS             4039..>4543
                     /locus_tag="VEP73_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYT26292.1"
                     /translation="MITEVRMPKLGMAMKKGAVTKWLKGEGDAVEEGADLFELATEKV
                     TAVVPAPATGRLGRVVAEPGTELPVGALLALIGEPGDAFPPADELAGAPPVTEPAAAA
                     AAPSAPAGRAAGAAPGGGGEGAPVSPAARRRARELGVDVALVPPSAPGKRVMVEDVEA
                     FAARAAGT"
BASE COUNT          587 a         1766 c         1561 g          629 t
ORIGIN      
        1 tgccagggtc catgtcgccc tcgacacagt ccacgaccac atcccggggg cagcatcgcg
       61 tcgggcacgg tcgaggaacg ctggggtggc gtccagcccg gtcatggggc agccatgctc
      121 ggccaggcgg ttggcgatgc ggccgtgccc acaggccacg tcgagcacgt ccataccggg
      181 gcgcacgccg acgtgggcca cgtgccagca gctgtatgac cttggagagc atctccacac
      241 aatctccacg aggtgcatgc cgatcctcga cctggcgtgc ctatgctcgg gaccatgcag
      301 ccaaggaccg tgctcgtcgt cgaggacgag gcctggacgg gctcgaggtg ctgcgctgca
      361 tccaggacaa gcgacccgtg cctgtgctca ggctgaccgc ccgcgactcc gagaccgact
      421 gctcgtcggg ctcgggctgg gcgccgacga ctacgtcacc aagccgttca gcccccgcga
      481 gctggtcgcc cgcgtccacg cgatcctgcg ccgcatcgag cgcgtccagg ccccgcccgg
      541 cgcgcccgtc cgcgtcggcg acgtcgagct ggacccgacc gcccgccgcg cccgccgcgg
      601 cggtgacccc gtccacctca ccccgaccga gttcgacctg ctgcacttcc ttgccgtccg
      661 gcccggcgtg gtgttcaccc gagagcagct cctggccgag gtctggggct ggcccaacgg
      721 ctccggcacg cgtacggtcg actcccacgt gcaggcgctc cgccgcaagc tgggcgcggg
      781 cgtggtgctc accgtccatg gcgtcggcta cgccgccccg gagggcgccc ggtgaggccg
      841 ctcgacccgc tctcctcgat caaggtcaag ctcggcgtgg tcatcctggc cgccgtcgcc
      901 gtcaccatcg tggtggagta cgttggtctg ctcctcgggg tcaacccctg gctgtccacg
      961 gtcggcgcgg ccgtgctcgc cctggccatg gtccagttcc tcgcccacgg catgacctcc
     1021 cccctgcgcg agatggcggc cgccgcccgg gccatggccc gcggcgacta caagcgccgg
     1081 gtcaccgcca gctcccgcga cgaggtcggt gagctggccc gcgccttcaa cgccatggcc
     1141 gccgtgctgg ccgagatcga gccgcccgat cccgagaccc tgcggaccat gctcaggcgc
     1201 tcaggcaggc cgagcgcctc ggcggcctga tcgtccagct cctcgccggc ctgtgcgccc
     1261 tgttcgccgc ctttaccgcc gcccagctcg tcgccctgtc cgagggcggc cggcacgtgc
     1321 tcgccaccgc cggcctcacc tacgccgagt acgcccgctc cggcctcttc cagctcctcg
     1381 ccgtcgccgt catcaccctg ggcgtcctgc tcctgctgcg cgccgccacc gacttggcca
     1441 gccccggccg gcgggccacc ttcaccgccg tcaccgagct ggccgtcgcc ctcaccctgg
     1501 tcgtcgtggt ggtcgccgtg cgccgcctca acctctcaac gtcgccaacc cggaggccgt
     1561 cgtcgcccgc gaccagctcc ccggcgtccg tcaggtccag cgcaccgatg tcgtctacgt
     1621 cgcccgcctc tctgaggacg ccgtccccac cgtggccgcc cgcctgcccg gcctggaccc
     1681 ggtcgcgcga gccgacctgc gcgcccggct ctgccccgtc gagcccgaca gcgtcccgtc
     1741 gtcccgcttc accggctggg ccgcttggaa ccttggccac gagcgcgccg agcgggccgt
     1801 cacccacatc tgcggctgac aaacagcgca tcacccctct ggcaagcccc ggcccgcgtc
     1861 gtgcggacca cgggctcgtc gtagccggtc tcggggccgt cccttcctga gggatccctc
     1921 ctgactcagg cgctgccgcg gcgtagactc ctcgcgggtg tcgggattgt ccacatgatg
     1981 gcaccaggat ggcaccagga gggacgccat ggcggagctc acccgggacg aggccctgtc
     2041 gatctacgac cggatggtga ccatcagagc cttcgaggag cgggttgcca agctcttcgc
     2101 cgacggccgg atccccgggt tcgtgcacct ctacgccggc gaggaggcgg tcgcggtcgg
     2161 cctgtgcgcc cacctcgagg accgtgactt catcacctcg acccaccgcg ggcacggcca
     2221 ctgcatcgcc aagggcgtcg agctcggccc gatgatggcc gagctgttcg gccgggtgga
     2281 cggctcctgc aagggcaagg gcggctcgat gcacatcgcc gacgtctccc gcggcatgct
     2341 cggcgccaac ggcatcgtcg gcggcggccc gccgctggcc tgcggcgcgg cgctgaccgc
     2401 caagcacaag ggcaccggcg ccgtgtgcgt gtgcttcttc ggcgacggcg ccgccgagca
     2461 gggcacgacc cacgaggcca tgaacctggc cgcgatctgg aagctcccgg tcgtcttcgt
     2521 ggccgagaac aacggcttcg ccgagtcgac cccggtcggc taccactgcg cggcgagcac
     2581 catcgccgac cgggccgcct cctacgacat ccccgggatc tcggtggacg gctacgacgt
     2641 cgtcgcggtc tacgaggcgg ccgaggaggc ggtgtcccgc gcccgccggg gcgacggccc
     2701 gagcctgatc gaggccaaga cctggcgcta cttcgggcac ttcgagggcg accaggtcac
     2761 ctaccggacc gccgagcagt cggccgccta ccgggagcac gacccgctga cgtacttcgc
     2821 caggcaggcg gtggaccgcg acctgctcac cgccgaccag gtcgaggagc gcaaccgggc
     2881 cgccgaggcc gccgtcgacg aggcgatcgc gttcgccgag cggagcccgg agccggcccc
     2941 cgaggacgcc ctcaccgacg tctacgtcac ctaccggtag aaggaggtcg gcgcatgtcc
     3001 gtcaccacgt ccagcgtcgc ccggacggcg accttccgcg aggcggtcaa cgaggccctg
     3061 cgcctcgaga tggaacgcga ccccgacgtg gtcctcatgg gcgaggacgt cgccgggggc
     3121 gccaccatcg agggcttcga gcgggaggac gcctggggcg gggtcctcgg ggtcaccaag
     3181 gggctcgtcc agcgcttcgg ccgcgagcgg gtgctcgaca ctccgatctc ggagtccggc
     3241 ttcatcggtg ccgccgtcgg cgccgccgcg accgggctgc ggccggtcgc cgagctgatg
     3301 ttcgtggact tcttcggcgt ctgcatggac cagatcttca accagggcgc caagctccgg
     3361 tacatgttcg gtggcaaggc ccgcgtgccc gtcgtgatcc gcaccatgat cggggccggc
     3421 ttccgggccg ccgggcagca ctcgggctgc cactactccg tcttcaccca catgcccggg
     3481 ctcaagaccg tggtgccgtc caccccggcc gacgccaagg ggctgctcgc cgcctcgatc
     3541 cgcgacgacg acccggtcct gttcttcgag cacaagctcc tctacgacac gaccggcgag
     3601 gtgccggagg gggagtacct cgtggagctc ggccgggccg acgtgaagcg cgagggcgac
     3661 gccgccaccg tggtcgccat cggccgcatg gtcaacctcg ccctcgaggc cgccgggacg
     3721 ctgtccgcct ccggggtcga ggtcgaggtc gtcgacccgc gcaccctgtc cccgctcgac
     3781 gacgaggcga tcctggcctc ggtccgcaag accggccggg tggtggtggt cgacgaggac
     3841 aacccgcgct gctcgatggc caccgacatc gccgccctgg tcgcctcccg tgccttcgac
     3901 cacctggacg ccccgccgca gctcgtcacc gcgccccaca cgcccgtccc gttctcgccc
     3961 gtgctcgagg acttctacgt cccgaccccg gagcgcatcg cggaagcggt ccaggccacc
     4021 ctgtagcgag gtccgcccat gatcaccgag gtgcgcatgc ccaagctcgg catggcgatg
     4081 aagaaggggg cggtcaccaa gtggctcaag ggggaggggg acgccgtcga ggagggcgct
     4141 gacctgttcg agctcgcgac cgagaaggtc accgcggtcg tgcccgcccc ggccaccggg
     4201 cggctcggca gggtggtggc cgagccaggg accgagctcc cggtcggcgc cctgctcgcc
     4261 ctcatcggtg agccaggcga cgccttcccg ccggccgacg agctggccgg cgccccgccc
     4321 gtcacggagc cggccgccgc ggccgctgct ccgtcggcgc cggccggccg cgccgccggg
     4381 gcggcaccgg gcggcggtgg cgagggggcg cccgtctccc cggcggcccg ccggcgggcc
     4441 agggagctcg gggtcgacgt cgccctggtc ccgccgagcg ctccaggcaa gcgggtcatg
     4501 gtcgaggacg tggaggcgtt cgcggcgcgt gcggccggga cgg
//
LOCUS       DASPYY010000471         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1205208, whole genome shotgun sequence.
ACCESSION   DASPYY010000471 DASPYY010000000
VERSION     DASPYY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205208"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /gene="hisD"
                     /locus_tag="VEP73_07430"
     CDS             <1..1274
                     /gene="hisD"
                     /locus_tag="VEP73_07430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYT26293.1"
                     /translation="LPRAPAADLAAARASVRALVADVATRGDPAVAEAARRFDGSETP
                     PARWRVSAGELAAAERALDPGLRAALADAIERVRAFHQAQRPADLVWTDPRRPGVRLA
                     QRFVPLARAGVYVPGGLGAYPSSVVMNVVPAQAAGVGSIALATPPGPGGHGHPAILGA
                     AAMLGVDEVWLLGGAQAVAALAGGTQTVPAADSVTGPGNRYVALAKQEVADRVRTDGH
                     AGPTEIAVLADHSADPRLVATDLIAQAEHDPLAACLLVTDDPAVWKAVEPVLTEEVAA
                     TRHRDRVERAFAGQSAVVLCDHGDRDAMLRVAEAFAPEHLELLVEGAAELAARVRNAG
                     AVFVGPWTPVALGDYAAGTNHVLPTAGTARFAAGLSTLSFLRAMQIAEFDRDALAAAA
                     PTVAALAEAEDLPAHGRAVAVRVGSASEVGA"
     gene            1271..>1539
                     /locus_tag="VEP73_07435"
     CDS             1271..>1539
                     /locus_tag="VEP73_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYT26294.1"
                     /translation="MNRLDGLDGRVRVRDDLADLTPYGAPQLDVPVRLNTNETPFPVP
                     QPVLDDIAEAVRGLELHRYPDREALELREALALHGGHGVEGTWAA"
BASE COUNT          173 a          603 c          578 g          185 t
ORIGIN      
        1 tgctgccacg cgccccggca gcggacctgg ccgccgcccg tgccagcgtc cgcgccctgg
       61 tcgcggacgt cgccacgcgg ggtgatcccg cggtggccga ggcggctcgc cgcttcgacg
      121 ggagcgagac gccaccggcg cgctggcggg tcagcgccgg cgagctggcg gcagccgagc
      181 gtgccctcga cccggggttg cgggccgccc tggccgacgc gatcgagcgg gtgcgtgcct
      241 tccaccaggc gcagcggccc gccgacctgg tctggaccga cccgagacgg cccggggtgc
      301 ggctcgcgca gcggttcgtg ccgctggccc gcgcgggggt gtacgtgccg ggcgggcttg
      361 gcgcctaccc ctccagcgtg gtcatgaacg tggtcccggc ccaggccgcc ggcgtgggga
      421 gcatcgcgct cgccaccccg ccagggccgg gtgggcatgg gcaccccgcg atcctcggcg
      481 ccgccgccat gctcggcgtg gacgaggtgt ggctgctcgg tggggcccag gcggtcgcgg
      541 cgctcgccgg cggcacccag accgtcccgg cggccgactc ggtcaccggg cctggcaacc
      601 gctacgtcgc ccttgccaag caggaggtgg ccgaccgggt ccgcaccgac gggcacgccg
      661 gcccgaccga gatcgccgtg ctcgccgacc acagcgccga cccgcgcctg gtcgccaccg
      721 acctgatcgc ccaggccgag cacgacccgc tggccgcctg cctgctggtc accgacgacc
      781 cggcggtgtg gaaggcggtc gagccggtcc tcaccgaaga ggtcgccgcc acccgccacc
      841 gcgaccgcgt ggagcgcgcc ttcgccgggc agtccgccgt ggtcctgtgc gaccacggcg
      901 accgcgacgc catgctccgg gtcgctgagg cgtttgcgcc cgagcacctg gagttgctgg
      961 tggagggcgc ggcagagctg gccgcgcggg tccgcaacgc cggtgcggtg ttcgtggggc
     1021 cgtggacccc ggtcgcgctc ggcgactacg cggctgggac caaccacgtt ctgcccaccg
     1081 ctgggaccgc ccgcttcgcc gcaggcctgt ccaccctgag cttcctgcgc gctatgcaga
     1141 tcgccgagtt cgaccgggat gctctcgccg ctgccgcccc gaccgtcgct gccctggccg
     1201 aggccgagga tctccccgcc cacggccgcg cggtggcggt ccgggtcggc agcgccagcg
     1261 aggtgggcgc atgaaccgcc tcgacgggct cgacgggcgg gtgcgggtcc gcgacgacct
     1321 ggccgacctc accccctacg gcgcaccaca gctcgacgtg ccggtgcggc tgaacaccaa
     1381 cgagaccccc ttcccggtgc cgcagccggt ccttgacgac atcgccgagg cggtccgcgg
     1441 cctggagttg caccgctacc ccgaccggga ggcgctggag ctgcgggagg cactggccct
     1501 gcacggcggc cacggcgtcg agggcacctg ggccgccaa
//
LOCUS       DASPYY010000472         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_220220, whole genome shotgun sequence.
ACCESSION   DASPYY010000472 DASPYY010000000
VERSION     DASPYY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220220"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VEP73_07440"
     CDS             complement(<1..595)
                     /locus_tag="VEP73_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HYT26295.1"
                     /translation="MRSDVHFTLPRELEAPAPPEARGLRRDRVRLLVVDRATGSIGHH
                     RFDELPSLLDPGDLLVVNDSRTLPASLPGRTASGTPLEVRLAGRVADRWAALPLGVPG
                     DGTDPALVATDARPAPPPLAPGERLRFGGGLTATVLGPHDEAPPLVWLAFDASGPALL
                     DALHRVGRPVRYAYVPRPWALHHYQTLFAAAPGSVEMA"
     gene            complement(604..1359)
                     /locus_tag="VEP73_07445"
     CDS             complement(604..1359)
                     /locus_tag="VEP73_07445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT26296.1"
                     /translation="MAVSSPGRLTGTTALVTGASQGLGRAIALAYAREGADLLLVARR
                     PEPLEAVRAEVEGLGARAVAVPADVGVPDDVERVAAAALAMFGRVDVLVNNASELGPT
                     PMPYLIDTDPAALERVLAVNVTGPFLLTRALLGPMLAAGRGSVINVSSDAGVVGYAGW
                     GSYGLSKAALDQLTRVWASELEGTGIRVNSVDPGDMATAMHAAALPDDDPATLARPED
                     RTEVFVHLAGPGSAGVTGQRLEAASFTPAAQGR"
     gene            complement(1740..2099)
                     /locus_tag="VEP73_07450"
     CDS             complement(1740..2099)
                     /locus_tag="VEP73_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HYT26297.1"
                     /translation="MAGDALQHLADVQEISIGFRRPDGSTGSTPVWVVQVGDNIFVRS
                     IRGPRGGWYRRLRANPNGEVRDAAHVHPVHAEPIADADTLAEVTCAYATKYAGSPYVQ
                     PLLEEPAIGATLRLGPS"
     gene            complement(2369..2782)
                     /locus_tag="VEP73_07455"
     CDS             complement(2369..2782)
                     /locus_tag="VEP73_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT26298.1"
                     /translation="MSSDFPTEGMELTHLLVVGDLERSREFYRDVLGAEVYREYGGTS
                     IVLRFLGTWLLLVTGGEPTEDKPDVTFAAPVDPRTVSHEMTIRVPDCQAAYATLRARG
                     AQFLTPPVHYDWEVRGFFRDPDGHLLEISEARTAG"
BASE COUNT          396 a         1030 c         1070 g          399 t
ORIGIN      
        1 tggccatctc gaccgacccc ggagcggcgg cgaacagggt ctggtagtgg tgcagcgccc
       61 agggccgcgg cacgtaggcg tagcggacgg ggcggccgac ccggtgcaga gcgtcgagca
      121 gggccgggcc gctcgcgtcg aacgcgagcc agacgagcgg cggcgcctcg tcgtggggcc
      181 ccaggacggt ggcggtcagg cccccgccga accgcagccg ctcacccggc gccaggggcg
      241 gcggcgccgg ccgcgcgtcg gtcgccacca gggcggggtc ggtgccgtcg cccgggacgc
      301 cgagcggcaa cgccgcccag cggtcggcga cccggccggc caggcggacc tccagcgggg
      361 tccctgacgc ggtgcggccg ggcaggctcg cggggagcgt ccgggagtcg ttcaccacca
      421 gcaggtcgcc ggggtcgagc agcgagggca gctcgtcgaa ccggtggtgc ccgatggagc
      481 cggtggcgcg gtcgaccacg agcaggcgga cgcggtcacg gcggagcccg cgcgcctcgg
      541 gcggcgccgg agcctcgagc tcccgcggga gggtgaagtg gacgtcgctg cgcaccccgg
      601 ccgtcaccgg ccctgggcgg ccggggtgaa cgaggccgcc tcgagccgct gcccggtcac
      661 ccccgccgac ccggggccgg ccaggtggac gaagacctcg gtgcggtcct cggggcgggc
      721 cagggtcgcc gggtcgtcgt cgggcagcgc cgccgcgtgc atcgcggtgg ccatgtcccc
      781 cgggtcgacc gagttgacgc ggatccctgt cccctcgagc tcgctggccc agacgcgggt
      841 gagctggtcg agggcggcct tggagaggcc gtaggagccc cagccggcgt acccgacgac
      901 gccggcgtcg ctcgagacgt tgatcacgct gcccctcccg gccgccagca tgggaccgag
      961 cagggcgcgg gtgagcagga acggcccggt cacgttgacg gccaggaccc gctcgagcgc
     1021 ggccgggtcg gtgtcgatca ggtacggcat cggggtgggg cccagctccg aggcattgtt
     1081 caccaggacg tcgacccgcc cgaacatggc cagggcggcc gccgcgacgc gctcgacgtc
     1141 gtcggggacg cccacgtcgg ccgggaccgc caccgcccgg gcgccgagcc cctcgacctc
     1201 ggccctgacc gcctccagcg gctcggggcg gcgggcgacg agcagcaggt cggctccctc
     1261 gcgggcgtag gcgagcgcga tcgcgcggcc gagcccctgg gacgcgcccg tgaccagcgc
     1321 ggtcgtacca gtcaggcggc cgggtgagga gacggccatc acggacctcc catggtcggc
     1381 ttcgtggtcc gcatggtccg cctggtccgg ctcttttgtc aacaaaactg ttgagaaact
     1441 atctgtggga cgccgagatc gcctacgcgt tccgggcgcg ggccatcctg gcccaaggtc
     1501 gcccgcggcc tgctcgatcg cgcttgacgc agccacggct gcttgggagt gcttgttgcg
     1561 ctggctacgc tcttgccttg acccgcgcct tcatgatcgc ccgcaccgtg agcagggcga
     1621 cgatcgctac cctaacggtc ggcgttccca acctacgcag gctcaccagg acgacgttgg
     1681 ctttccggcc gccccaggct gcggatctgg cggttggagg ttcgccttct agaacttggc
     1741 tagctcgggc cgagacgcag tgtcgcgccg atggctggct cctcgagaag cggctgcacg
     1801 tacgggctgc ccgcgtactt ggtcgcgtag gcgcaggtga cctcggcgag cgtgtcggcg
     1861 tccgcaatcg gctcggcgtg caccggatgc acgtgcgcgg catcccgcac ctcgccgttg
     1921 ggattggcgc gcagccggcg gtaccagccg cccctggggc cgcgtatcga gcgcacgaag
     1981 atgttgtcgc cgacctgcac gacccatacc ggggtggagc cggtggaacc gtccgggcgc
     2041 cggaagccga tgctgatctc ctgcacgtcc gcgaggtgct gcaacgcgtc acccgccatt
     2101 gtgtccacct ccgcgaccgg ggcgtctcgg tcggcccacc tggctaaacc ggtgcctcgt
     2161 tgaggcaacc agggtgcgga ccgtactcca ccgggtgcgg accggccacc cgtcgactcc
     2221 gaccagatcg ccgccctgca ttgtccagtg cgtccgaggc ggcaccgacc gcccgccgcg
     2281 gtggcatgcg tgcctttccc cggtctccgt catgaggacg agtggcggct gaggacgact
     2341 gtaggtgatg cctgtgcctg gggaacggtc atccagcagt cctcgcctcg ctgatctcca
     2401 ggaggtgacc atcagggtcg cggaagaagc cgcgcacctc ccagtcgtag tggacgggcg
     2461 gggtgagaaa ctgcgcaccc cgcgctcgaa gcgtcgcgta ggccgcctgg cagtccggga
     2521 cacggatggt catctcgtga ctgacggtgc ggggatcgac cggcgcggcg aaggtaacgt
     2581 ctggcttgtc ttcggtcggt tcaccgccgg tgaccagcag cagccaggtc cccaggaagc
     2641 gcagcacgat ggacgtgccg ccatactctc ggtacacctc agcccccagg acgtcgcggt
     2701 agaactcccg ggatcgctcc aggtcgccca cgaccagtag gtgcgtgagc tccatcccct
     2761 cggtcgggaa gtcgctgctc atggcccccc cccttcgcct cgctgccgtc gaccgcgaga
     2821 gtctacggag tgtcgaggcc catgacagat caccccgctc ggccgggtcg gagccgaggg
     2881 agagcgtgag cctca
//
LOCUS       DASPYY010000473         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1485725, whole genome shotgun sequence.
ACCESSION   DASPYY010000473 DASPYY010000000
VERSION     DASPYY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485725"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..469)
                     /locus_tag="VEP73_07460"
     CDS             complement(260..469)
                     /locus_tag="VEP73_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26299.1"
                     /translation="MQASTGGITDDVPVASTTARRAVRRRTGPSAARASTTRAPASLP
                     APRTSSMPLRSSQGSWPSSCQLEVM"
     gene            464..619
                     /locus_tag="VEP73_07465"
                     /pseudo
     CDS             464..619
                     /locus_tag="VEP73_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
     gene            688..927
                     /locus_tag="VEP73_07470"
     CDS             688..927
                     /locus_tag="VEP73_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26300.1"
                     /translation="MAGAYTLGNGISGTSQLVVDDARRVVVGATFTGPGLQELLHSAT
                     IAIVGQVPLDRLWHAVPSFPMVSEVWLSLLEAYGL"
     gene            complement(1003..1311)
                     /locus_tag="VEP73_07475"
     CDS             complement(1003..1311)
                     /locus_tag="VEP73_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HYT26301.1"
                     /translation="MSAAWVTVVLVGLATFAIKAAGPLLLAGRRLPDRLQGALDHLAP
                     ALLAALVATEALGSQGRLALDPRLLGLGAAAVALALRAPTLLVLVVAAGTTGLARALL
                     "
     gene            complement(1308..>1714)
                     /locus_tag="VEP73_07480"
     CDS             complement(1308..>1714)
                     /locus_tag="VEP73_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HYT26302.1"
                     /translation="TAAALSGPRWRTALTAQLVVDESWALAQRQGGRIDRSVLLGAGL
                     TLYVSWLAGTAAGVVAGAFVGDPARLGLDAAFPALFLALLVPQLRSRSRLLAAAGGAA
                     VALALTPVASPGVPLLAATVACLFGAGARRPA"
BASE COUNT          216 a          624 c          700 g          174 t
ORIGIN      
        1 ttcgtgctgc gacagtacag cgacctggag gcgctggagg cgttcaccca gcaggccggg
       61 gaagccccgg cgtaggaggc gcggccgtgg ccctgatcga gcgggagctg gtgggcgggg
      121 agtgctcctt ctggggctgc atcccgtcca agaccctcat ccgtcccggc gacgcgatcg
      181 cggcggcccg tcgggtgccc ggagcggccg aggctgtgac cggcccgatc gacgtgggcg
      241 ccgccttcgc ccagcgcgac tacatgacct cgagctggca cgacgacggc cagctcccct
      301 ggctcgagcg caagggcatc gagctggtgc gtggcgccgg caggctggcc ggcgcgcggg
      361 tcgtcgaggc ccgggcggcc gacgggccgg tccggcgcct gaccgcgcgc cgggcggtgg
      421 tgctcgccac cggcacgtcg tcggtgatcc cgccggtcga ggcctgcacg aggtcgagcc
      481 atgggacaac cggggcgtca ccgcggccaa ggagctcccc gcccgcctgc tggtcctcgg
      541 cggcgacacc gtcggcgccg agatggccca gtgctacaag cgcctcggca gcgcggaggt
      601 cacggtcgtc gaggaaggcc cgcggctgct cgcccgggag gagccgttcg ccggcgagga
      661 ggtcgcccgg gccttcgcgg ccgatcggtg gccggcgcct acacgctcgg caacggcatc
      721 tccggcacca gccagctggt ggtcgacgac gcccgccggg tcgtcgtcgg tgccaccttc
      781 accggtcctg gcctgcagga gctgttgcac tcggccacca tcgcgatcgt cggccaggtc
      841 ccgctcgacc ggctgtggca tgccgtgccc tcgttcccca tggtcagcga ggtctggctc
      901 agcctcctcg aggcctacgg gctctgagcc gcccggcgag gcacgggacc cgcgggtggc
      961 cgcctgggcg catcggtccc gggcggcacc tgccggcgag gctcacagga gcgcgcgggc
     1021 gagcccggtc gtgccggcgg cgaccaccag cacgagcagg gtcggggcgc gcagcgccag
     1081 ggcgaccgcg gccgcgccga ggccgagcag gcgggggtcg agcgccaggc ggccctggct
     1141 cccgagcgcc tcggtcgcca ccagcgccgc gagcagcgcg ggcgccaggt ggtcgagggc
     1201 gccctgcagg cggtcaggga gccgccggcc ggcgagcagg aggggaccgg cggccttgat
     1261 ggcgaaggtc gcgagcccga ccaggaccac ggtgacccag gccgcgctca tgcgggcctc
     1321 cgggcgcccg cgccgaacag gcaggcgacg gtggcggcga ggagcggcac gccggggctg
     1381 gccaccgggg tgagggccag cgcgaccgcg gcgccgccgg ccgcggcgag caggcgcgac
     1441 cgcgagcgga gctgggggac gagcagggcc aggaagaggg ccgggaaggc ggcgtccagc
     1501 ccgaggcgcg cagggtcgcc gacgaacgcg cccgcgacca cgccggcggc ggtcccggcc
     1561 agccacgaca cgtacagggt gaggcccgcc ccgagcagca cggaccggtc gatgcggccg
     1621 ccttggcgct gcgcgagagc ccaggactcg tccacgacga gctgggccgt gagcgcggtg
     1681 cgccagcgcg ggccgctgag cgcggcggcg gtgg
//
LOCUS       DASPYY010000474         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1344530, whole genome shotgun sequence.
ACCESSION   DASPYY010000474 DASPYY010000000
VERSION     DASPYY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344530"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2093
                     /locus_tag="VEP73_07485"
     CDS             <1..>2093
                     /locus_tag="VEP73_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HYT26303.1"
                     /translation="RPLVRASARRGGVRAVPPRQPAWLREVAAAVQAEIEARPGVPAR
                     VDLGPARPEPSTERALTATFRVDLRGVRVDVASLTDLWLAPPGDPACRSEVAEIVAHA
                     GDLLLVRATAQPGDQLHLDHVHLFATSDPLPPLRALLDALGRVRRPGLAERFDSKVLD
                     PVPPGPHVRPGLGPEEAAAWAAGCSAGFNLVWTPPGTDKTRVIAEVVAQLALTGRRVL
                     LVSATAAAVDQAVLEASRVMAGAWPGRIVRVGHPSLLELAEHPFLRLERACESLADGH
                     RGRLAEVERRVADLRAEPDLLVLERAERLLAGFDGPAYELASVLVANAERLATLQGRL
                     DERVSALAAAGAERVAAAAEVERTDAAIAETRQARRAYQQADTLQRELDAIAGSLHAA
                     REAVRLIKERLAQTRRASAEHAELPVVRRVARLGAANRHRLAQRELEAELVPAWEQVV
                     RLERRAGQVREQAEGLEESARPFTREDLARRDLARERALERLDAAVRAERDHDRARRE
                     LGHEVSSAARAPQPSPADVELVRAADEQSLPELAARAAELRAAARPITDELDRLGAER
                     RRLAADVERLAPAVVADAAVVATTLATLASDPAVRGRPYDHVLVDEASAALPPHVVYA
                     ATRAGAGVTLFGDFLQNGPPSAIESSKALPVSRWLLRDSFAVLGIDRPARALASRGCA
                     VLGEQQRLGPATAEL"
BASE COUNT          226 a          864 c          776 g          227 t
ORIGIN      
        1 cgagaccgct cgtcagggcg tcggcgcggc ggggcggcgt ccgggccgtc ccgcccaggc
       61 agcccgcatg gctccgcgag gtcgccgccg ccgtccaggc cgagatcgag gccaggcccg
      121 gcgtgcccgc ccgggtcgac ctcggcccgg cccggcccga gccgtcgacc gagcgggcgc
      181 tcaccgcgac gttccgcgtc gacctgcgcg gcgtgcgggt cgacgtcgcc tccctcaccg
      241 acctctggct cgccccgccg ggcgaccccg cctgccgttc ggaggtggcc gagatcgtcg
      301 cccacgccgg cgacctgctg ctcgtgcgcg cgacggcgca gccgggcgac cagctgcacc
      361 tcgaccacgt ccacctcttc gccaccagcg accctctccc gccgctccgc gccctgctcg
      421 acgcccttgg cagggtgcgc cggccgggcc tggccgagcg cttcgactcg aaggtgctcg
      481 accccgtccc gcccggtccc cacgtcaggc ccgggctcgg ccccgaggag gcggcggcct
      541 gggccgccgg ctgctccgcg gggttcaacc tcgtctggac gcctcccggc acggacaaga
      601 ccagggtgat cgccgaggtg gtcgcccagc tcgccctgac cgggcggcgc gtcctgctcg
      661 tctcggccac cgccgcggcg gtggaccagg ccgtgctgga ggcgtcccgc gtcatggccg
      721 gcgcctggcc gggccggatc gtccgggtcg gccacccgag cctcctcgag ctggccgagc
      781 atcccttcct ccgcctcgag cgggcctgcg agtcgctcgc ggacggccat cgcggccgcc
      841 tggccgaggt cgagcgccgc gtcgccgacc tgcgtgcaga gcccgacctg ctggtcctcg
      901 agcgggccga gcggctcctg gccggcttcg acggccctgc ctacgagctc gccagcgtgc
      961 tggtggcgaa cgccgagcgg ctcgccaccc tgcagggacg gctcgacgag cgggtgtccg
     1021 ccctcgcggc cgccggcgcc gagcgcgttg cggccgcggc cgaggtcgag cggaccgacg
     1081 ccgccatcgc ggagacgcgg caggcacgcc gcgcctacca gcaggccgac accctgcagc
     1141 gcgagctgga cgcgatcgcc ggctccctcc acgccgcccg cgaggcggtc cgcctgatca
     1201 aggaacggct cgcccagacg cggcgggcgt ccgccgagca cgccgagctg ccggtggtga
     1261 gaagggtcgc gaggctcggg gcggccaacc gccaccggct ggcccagcgc gagctggagg
     1321 ccgagctggt gcccgcctgg gagcaggtcg tccggctgga gcgcagggcc ggccaggtcc
     1381 gcgagcaggc cgagggcctc gaagagagcg ccagaccgtt caccagggag gacctcgcgc
     1441 ggcgggacct cgcgcgcgag cgggccctcg agcggctgga cgccgcggtc cgcgccgagc
     1501 gcgaccacga ccgggcgcgc cgggagctgg gccacgaggt gagctcggcg gcgcgggcac
     1561 cccagccctc accggccgac gtcgagctgg tccgcgccgc cgacgagcag tcgctgcccg
     1621 agctcgccgc ccgcgccgcc gagctgcggg cggcggcccg cccgatcacc gacgagctcg
     1681 accggctcgg cgccgagcgg cggcgcctcg ccgccgacgt cgagcgcctg gccccggcgg
     1741 tcgtcgccga cgccgccgtc gtcgccacga cgctcgccac gctggccagc gatcccgccg
     1801 tccgcggccg gccctacgac cacgtcctgg tcgacgaggc gtcggcggcc ctgccgcccc
     1861 acgtcgtcta cgcggccacc cgggccggcg ccggcgtgac cctcttcggc gacttcctgc
     1921 agaacggccc gccgagcgcg atcgagtcct cgaaggcact gccggtctcc cggtggctct
     1981 tgcgggacag cttcgccgtg ctcgggatcg accggccagc ccgcgccctc gccagccggg
     2041 ggtgcgccgt gctcggcgag cagcagcggc tcggcccggc aaccgcagag ctc
//
LOCUS       DASPYY010000475         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_782052, whole genome shotgun sequence.
ACCESSION   DASPYY010000475 DASPYY010000000
VERSION     DASPYY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782052"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VEP73_07490"
     CDS             complement(<1..456)
                     /locus_tag="VEP73_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYT26304.1"
                     /translation="MTGEQRMLATCRGGEVDATPVWFMRQAGRCLRGYRELRERHGIL
                     EITRTPDLCAQVTLMPVDVFGVDAAVMYADIMLPLFGMGVPFSIDPGVGPIVHEPVRD
                     EAAVAALEVVEAEDATPELFEAIRLVRRELAGRAALIGFAGAPYTVASYL"
     gene            649..1605
                     /locus_tag="VEP73_07495"
     CDS             649..1605
                     /locus_tag="VEP73_07495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYT26305.1"
                     /translation="MEAGDADGPLVFGGSASPRLTKEICGHLGVAPGQGEVLKFSEGT
                     LFPRVLDNVRGRRAYLVQSTVFPANDHFMELLFWVDALKRASAASVTVVMPYFSYAKG
                     DKKDEPRVSIRARVCADAIEAAGADRVVTMDLHAPQIQGFFRIPVDDLYARPLLCDAI
                     RAEGLAAPVIVSPDAGFAKKARMFAAALGAPLAIADKMRADHTERAHVIDVIGEVAGR
                     DAVVVDDFTISAGTLVEAAEQLVAKGARSVMAAVSHGVFAAGSMERLDSSPIRRLVTT
                     DTIETQPVGLSDKVRVVSVAPLFAEAIRRIHHRESISVLFER"
     gene            complement(1621..2460)
                     /locus_tag="VEP73_07500"
     CDS             complement(1621..2460)
                     /locus_tag="VEP73_07500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT26306.1"
                     /translation="MTARPASRPGALLDRLEAYYDAVPRAAARAETLGPLVLFVTEGP
                     GWPYYARPRRCAVAVTAEDVWAMRRLQRALGVPEAFEWVDEVTPSLKAAAGEAGLAVA
                     ELPLMAVASAAVRRPRAPAGAEVRLVIPGDDLALVTAVAQVGFSWPGTGAGGAGPDAL
                     AAAAAAVTPQRLAFLRERLAAGRTVTAVALVDGLPVAVGSHQPVGGVTEVVGVATLPA
                     MRRRGLGAAVTGLLVEDAARCGIATAFLSAGDAAVARVYARLGFERVGTACIAEPAGG
                     HQV"
     gene            complement(2457..2792)
                     /locus_tag="VEP73_07505"
     CDS             complement(2457..2792)
                     /locus_tag="VEP73_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26307.1"
                     /translation="MAESRQAWDEVSESFAALGRRVKQHYEQRGDQGDAADRKAAEDA
                     LRRLADSLDQTFTAVGNAVRDPAFGEETRKAASKLGEALSATFAEMSDDLRERFKSRG
                     RGPGGPATP"
     gene            3010..>3894
                     /locus_tag="VEP73_07510"
     CDS             3010..>3894
                     /locus_tag="VEP73_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26308.1"
                     /translation="MSNTQTIESRSPQDPSDVVATVPDSGTDAVARAVRRAREAARGW
                     AATPAVQRASMLNAAADRLAAAHDEVSGLVVREVGKPVGEARQEVTRGVGILRYHAQA
                     ALDPDGETYPLAPPADRTSLLMSRRRPRGVAGLITPWNFPVAIPLWKAAPALAYGNAA
                     VLKPAPESSALGVRLGELLGGVLPEGVLTVVTGGGEAGRALVELADVASFTGSVGAGF
                     SVARAAAERGIAVQAEMGGQNASIVLPDADLETTASVVAGAAMGYAGQKCTATSRVIV
                     VGDPDRVAAFTGALVAAVE"
BASE COUNT          496 a         1493 c         1455 g          450 t
ORIGIN      
        1 gaggtaggac gcgaccgtgt agggcgcgcc ggcgaagccg atcagcgcgg cccggccggc
       61 gagctcccgc cggaccagcc ggatcgcctc gaacagctcg ggcgtcgcat cctccgcctc
      121 caccacctcg agggccgcga ccgccgcctc gtcccgcacg ggctcgtgca cgatcggccc
      181 gacgcccggg tcgatggaga acggcacgcc catgccgaag agcggcagca tgatgtcggc
      241 gtacatgacc gcggcgtcca cgccgaagac gtcgaccggc atcagggtga cctgcgcgca
      301 caggtccggg gtcctggtga tctcgaggat cccgtggcgc tcgcgcagct cccggtaccc
      361 gcggaggcag cgcccggcct gccgcatgaa ccagaccggc gtggcgtcga cctccccgcc
      421 acggcacgtg gcgagcatgc gctgctcgcc cgtcatgccc ggctccccgg tcgctggcgg
      481 caacgtctct ggcggcaacg gacctccctc gtcgacggcg tcacgagcaa agggtacgcg
      541 aggccgggcg ggaaggacgg tggtgcgggc ccgcgtggcg cgtgtagggt cgagccgaca
      601 ccgggcgcca ccggtgccgg gcgagcgacg ggcgagggac ggagcggcat ggaggcaggc
      661 gatgcggacg ggccgctcgt cttcggcggc tcggcgagcc cgcggctgac caaggagatc
      721 tgcggccacc tcggcgtcgc gcccggccag ggcgaggtcc tgaagttctc ggagggcacc
      781 ctgttccccc gggtgctcga caacgtccgc ggccgccgcg cctacctggt gcagtcgacc
      841 gtcttcccgg ccaacgacca cttcatggag ctgctcttct gggtcgacgc gctcaagcgg
      901 gcgagcgcgg cgtcggtgac ggtcgtcatg ccgtacttca gctacgccaa gggtgacaag
      961 aaggacgagc cacgggtgtc gatccgcgca cgcgtctgcg ccgacgcgat cgaggcggcc
     1021 ggcgccgacc gggtcgtcac catggacctg cacgcgcccc agatccaggg cttcttccgc
     1081 atcccggtcg acgacctgta cgcccgcccc ctgctgtgcg acgcgatccg ggccgagggc
     1141 ctggccgcgc cggtgatcgt ctcccccgac gccggcttcg cgaagaaggc caggatgttc
     1201 gcagccgccc tcggcgcgcc gctcgcgatc gccgacaaga tgcgcgccga ccacaccgag
     1261 cgggcgcacg tgatcgacgt gatcggggag gtcgccggcc gggacgccgt cgtcgtcgac
     1321 gacttcacga tcagcgccgg caccctggtc gaggcggccg agcagctggt ggccaagggc
     1381 gcccgctcgg tgatggccgc cgtcagccac ggggtgttcg ccgccgggtc gatggagcgg
     1441 ctggactcga gccccatccg gcggctcgtc acgaccgaca cgatcgagac ccagccggtg
     1501 gggctctcgg acaaggtgcg ggtggtgtcg gtggccccgc tgttcgccga ggccatccgg
     1561 cgcatccacc accgcgagag catcagcgtg ctgttcgagc gctgacccgg gcaggtccgc
     1621 tcagacctgg tggccgccgg ccggctcggc gatgcaggcg gtgccgacgc gctcgaagcc
     1681 gagccgcgcg tacacccggg cgaccgccgc gtcgccggcg gagaggaacg ccgtggcgat
     1741 gccgcacctg gccgcgtcct cgacgagcag cccggtcacg gccgccccga gcccgcgccg
     1801 gcgcatggcg ggcagggtgg cgaccccgac cacctcggtg acgccgccga ccggctggtg
     1861 gctgccgacc gcgaccggca ggccgtcgac cagcgcgacc gcggtgacgg tgcgcccggc
     1921 cgcgagccgc tcccgcagga aggccagccg ctgcggggtg accgcggcgg ccgcggcggc
     1981 cagggcgtcc gggccggcgc cgcccgcccc cgtccccggc caggagaagc cgacctgggc
     2041 gacggccgtc accagcgcga ggtcgtcgcc ggggatgacc aggcggacct cggcgccggc
     2101 cggtgcccga gggcggcgca ccgcggccga ggccaccgcc atgaggggca gctcggccac
     2161 ggcgagcccg gcctccccgg ccgcggcctt caggctcggc gtgacctcgt cgacccactc
     2221 gaacgcctcg ggcacgccga gggcgcgctg gaggcggcgc atcgcccaca cgtcctcggc
     2281 cgtgaccgcc accgcgcagc gccgcggcct ggcgtagtag ggccagccgg gcccctcggt
     2341 cacgaacagc accaggggcc cgagcgtctc cgcgcgggcc gccgcccggg gcacggcgtc
     2401 gtagtaggcc tcgaggcggt cgagcagggc gcccggccgc gacgccgggc gagcggtcac
     2461 ggcgtggccg ggccccccgg tcccctgccc ctgctcttga agcgctcgcg gaggtcgtcg
     2521 ctcatctccg cgaaggtcgc cgagagggcc tcgcccagct tggacgccgc ctttctcgtc
     2581 tcctccccga acgccgggtc ccgcacggcg ttccccaccg ccgtgaacgt ctggtccagt
     2641 gagtccgcga gcctgcgcag ggcgtcctcc gcggccttgc ggtcggcggc gtcgccctgg
     2701 tccccacgct gctcgtagtg ctgcttcacc cggcggccga gcgccgcgaa gctctcgctg
     2761 acctcgtccc atgcctgcct gctctcggcc atctgctccc tccagcccgt cggcccgccc
     2821 gggccgccac gccgccgatc atacgcacgt gccgaccagg gcggcagcgg cgcgcccacg
     2881 cactgtgccg tgcatccgtg gcacctttag gcagaactga acgacgaggc ggaactgaac
     2941 gacgaggccg gggcgggccg gacgacgggg cgcgccgaac gacggtcccg gcccgggacg
     3001 ggagtcgcca tgagcaacac gcagaccatc gagagcaggt caccgcagga cccgtccgac
     3061 gtcgtggcca ccgtgcccga ctcgggcacc gacgccgtcg cgagggccgt ccggcgggcg
     3121 cgcgaggcgg cccgcggctg ggcggccacc ccggccgtgc aacgggccag catgctgaac
     3181 gcggccgccg accggctcgc cgccgcccac gacgaggtca gcggcctggt cgtgcgcgag
     3241 gtcggcaagc ccgtcggcga ggcgcgccag gaggtgaccc gcggcgtcgg gatcctccgg
     3301 taccacgccc aggccgccct cgacccggac ggggagacct acccgctggc ccctccggcg
     3361 gaccggacgt cgctgctcat gagccgcagg cggccccgcg gcgtggccgg gctgatcacc
     3421 ccgtggaact tccccgtcgc gatcccgctc tggaaggcgg cccccgcgct cgcctacggc
     3481 aacgccgccg tgctgaagcc ggcgcccgag tcgagcgcgc tcggcgtccg cctcggggag
     3541 ctcctcggcg gggtcctccc cgagggggtg ctcaccgtcg tcacgggggg tggcgaggcc
     3601 ggcagggccc tcgtcgagct ggccgacgtc gcgagcttca ccgggtcggt cggcgccggg
     3661 ttctcggtcg cccgcgccgc ggccgagcgg ggcatcgcgg tgcaggccga gatgggcggc
     3721 cagaacgcct cgatcgtgct gcccgacgcc gacctcgaga ccacggcgtc ggtggtcgcc
     3781 ggcgcggcga tgggctacgc gggccagaag tgcacggcga cgagccgcgt gatcgtggtc
     3841 ggcgacccgg accgggtcgc cgccttcacc ggggcgctgg tcgcggcggt ggag
//
LOCUS       DASPYY010000476         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1625204, whole genome shotgun sequence.
ACCESSION   DASPYY010000476 DASPYY010000000
VERSION     DASPYY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625204"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VEP73_07515"
     CDS             complement(<1..313)
                     /locus_tag="VEP73_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26309.1"
                     /translation="MEETGEQARGQLGALHPGASATPLPAHGPELRTADGRLAAWVFD
                     LPVAELRAGYRAGVYFDRTRRILAAEGADTRVTMQVFQRADETVACGIDEALAILALG
                     AG"
     gene            439..1203
                     /locus_tag="VEP73_07520"
     CDS             439..1203
                     /locus_tag="VEP73_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HYT26310.1"
                     /translation="MLAHKLAGPTAQVVVVQLDAGQTAWCEAGRLVWKTANVDLRARL
                     SGPVGRDAAGVLGRAVAAVVVVGRRRLAGERPAYHHLSVPEGSGLAAFASPAPGELRE
                     LRLDGTRGWLVARRAVVVAEGTVGPDLALTVRGLAGSDGDPPDLEGLAGVGSLFVAAS
                     GSLVELDPARFGGQVQADATRLVAVQEGVGVTVERVGALPGPEVVASVLGGEPVALAT
                     LSGAGAVLLQSASSPATAMHPDDREPEEGRWHSPTT"
     gene            1182..1514
                     /locus_tag="VEP73_07525"
     CDS             1182..1514
                     /locus_tag="VEP73_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26311.1"
                     /translation="MALADDVRNLAGQAQRGLEQGAQQVQDKLDELRLRRRFNELARQ
                     LGRAVYQGRKQGGADETEVARICAQMAAVEAELAAGRRRGSDGPDRSPVLPGAERPPA
                     GGHTLDDV"
     gene            1596..>1748
                     /locus_tag="VEP73_07530"
     CDS             1596..>1748
                     /locus_tag="VEP73_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYT26312.1"
                     /translation="MTRFQRLAAATTVATFALVAVGGLVRATDSGLGCPDWPRCFGRL
                     MPPAELH"
BASE COUNT          196 a          679 c          678 g          195 t
ORIGIN      
        1 ccccggcgcc gagggcgagg atcgccaggg cctcgtcgat cccgcacgcc acggtctcgt
       61 cggcgcgctg gaacacctgc atggtgaccc gcgtgtcggc tccctcggcg gccaggatgc
      121 gccgggtgcg gtcgaagtag acgcccgccc ggtagccggc gcgcagctcg gcgaccggga
      181 ggtcgaagac ccacgcggcc agccgcccgt ccgccgtgcg cagctcgggg ccgtgcgccg
      241 gcagcggggt ggcggacgcg ccggggtgca gggcgccgag ctgccccctg gcctgctccc
      301 cggtctcctc catggtggca ccctagcgga tcgagggctg ccgcgccgga cccgttgcca
      361 ccccggcgac ccggcggtag cgtcggggca ggagccccgt ggcaacgctc cggggcacga
      421 ccggaagcga ggcgggccat gctcgcgcac aagctcgccg gcccgaccgc ccaggtggtg
      481 gtggtccagc tcgacgccgg gcagaccgcc tggtgcgagg cgggccggct ggtctggaag
      541 accgccaacg tcgacctgcg cgcccggctc tccgggcccg tcggccggga tgccgccggc
      601 gtgctcggcc gggcggtcgc agccgtcgtc gtggtgggca ggcgccgtct cgccggcgag
      661 cgcccggcct accaccacct cagcgtgccg gaagggagcg gcctggccgc gttcgcctcg
      721 ccggcccctg gcgagctgcg cgagctgcgg ctcgacggta ccaggggatg gctcgtggcg
      781 aggcgcgccg tggtcgtcgc cgaggggacg gtcgggcccg acctcgccct gaccgtccgc
      841 gggctggccg gctcggacgg cgacccgccc gacctcgagg gcctcgccgg cgtcggctcg
      901 ctgttcgtcg cggcctccgg gagcctcgtg gagctcgacc cggcccgctt cggcgggcag
      961 gtccaggccg acgcgacccg gctggtcgcc gtccaggagg gcgtcggcgt caccgtcgag
     1021 cgggtcggcg cgctgcccgg accggaggtc gtggccagcg tcctcggcgg cgagccggtc
     1081 gcactcgcca ccttgtcggg cgccggcgcg gtcctgctgc agtctgcgag ctccccggcg
     1141 accgcgatgc acccagacga ccgcgaacca gaggagggcc gatggcactc gccgacgacg
     1201 tgaggaacct ggccgggcag gcccagcggg gcctcgagca gggcgcccag caggtgcagg
     1261 acaagctgga cgagctccgc ctccggcggc gcttcaacga gctcgcccgc cagctgggcc
     1321 gggccgtcta ccagggccgc aagcagggcg gggcggacga gaccgaggtg gccaggatct
     1381 gcgcccagat ggccgccgtc gaggccgagc tggccgccgg gcgccggcgg ggctccgacg
     1441 gcccggaccg ctcgccggtc ctgccgggcg ccgagcgccc gcccgccggc gggcacaccc
     1501 tcgacgacgt gtagcgcccc tcgcgctggt agcatcgacg tgctcgccag gcaggcagga
     1561 gccttgccgg cacggcccac cagcccagga gaacggtgac ccggttccag cgcctcgccg
     1621 ccgccaccac ggtggcgacg ttcgccctcg tcgccgtggg agggctggtc cgggcgaccg
     1681 actccggcct cggctgcccc gactggcccc gctgcttcgg caggctcatg cccccggccg
     1741 agctccac
//
LOCUS       DASPYY010000477         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1344769, whole genome shotgun sequence.
ACCESSION   DASPYY010000477 DASPYY010000000
VERSION     DASPYY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344769"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VEP73_07535"
     CDS             <1..198
                     /locus_tag="VEP73_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYT26313.1"
                     /translation="LAGTLTRVEVAPGDQVAAGDLLAVVEAMKMETPLLAPFTATVAA
                     VPSQAGGPVSAGQVVVVLEPS"
     gene            complement(195..974)
                     /locus_tag="VEP73_07540"
     CDS             complement(195..974)
                     /locus_tag="VEP73_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYT26314.1"
                     /translation="MDPIVVEVAEGIATVTLQRPEALNAISTELALALAEAVEPLAVK
                     QSVRAVVLEGAGERAFCVGADLRQRGGFTDHDWFEQREVFRRAFGALRRCPLPTVAAV
                     FGFALGGGMELALACDLVVAADDAVFGLPEVRLGLVPAGGGTQLLVRRVGRSVAKDLV
                     LTGRRVDAAEALRLGIADRVVSRAALPEAARGLAAEIAANSPSAVRLAKWALDLGADL
                     AQEAAIEVEDQAWRRAVLSDDRREGIAAWNERRSPNWPNWP"
     gene            complement(980..1948)
                     /locus_tag="VEP73_07545"
     CDS             complement(980..1948)
                     /locus_tag="VEP73_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010540123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT26315.1"
                     /translation="MRTLVTGGAGFIGSALVDRLAHHGHEVTVVDDLSTGKLDNLVDA
                     FGSGRVEHVEADVAGPELAAIVAEARPEVVFHLAAQIDVRRSVADPAHDARVNVLGSV
                     ALAEAAVENGARRLLFAGSGGTAYGEPDPGDLPVAEDYPPRPTSPYGVAKRSAEDYLA
                     SFRDIHGLEWVSLRLGNVYGPRQDPHGEAGVVAIFCNRLLSGEPVTIFGDGLQTRDYV
                     YVEDVVNAFLLAGERDEALGARVNIGTGVETSVLDLYATLREVTGFGPEPQFAPPRSG
                     ELQRIALDCGEAERVLRWQRNVAFADGLARTWEWAFQQVNAGSVGG"
     gene            2095..>3137
                     /locus_tag="VEP73_07550"
     CDS             2095..>3137
                     /locus_tag="VEP73_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYT26316.1"
                     /translation="MSEQIEERDEAPPAAPQARLGAAVRTMTRGWALPYLVVLVVAAA
                     DLVVGGDLSMVAFLVLGPLLASRLSTDRRQVLAAGTVAFAAGVLLGEFNRDLTTGQQA
                     LRLLLIGVGTLIALINQGALRREQSALERAADTVALAASLVAGVEPEEAYRLLARSAR
                     SLYEADAAAVYRRDADQLLLAADDRHGLIPPMPGWLGRTNVPDAFASGPVEATVRSAT
                     APEATMLAARGLGSVLWLPLLDPAGRQTGVIALAWRRPDPRLSAEALGASRQFADLGG
                     RAISGSERVRAQTEILERVQALLLSTPPAWAQGYRIGVRYQSASGLAQIGGDFYDVVE
                     VEDGKGLAFILADA"
BASE COUNT          391 a         1215 c         1129 g          402 t
ORIGIN      
        1 ctggccggca ccctgacccg ggtcgaggtc gcccctggcg accaggtggc cgccggggac
       61 ctgctcgcgg tagtggaggc gatgaaaatg gagacgccgc tgcttgcccc gtttacggcc
      121 acggtggcgg cggtcccctc ccaagcagga ggccctgtct ccgccggtca ggtcgtcgtc
      181 gtactggagc ccagctaggg ccagttaggc cagtttgggc tgcggcgttc gttccaggcg
      241 gcgatgccct cgcggcggtc gtcggagagg accgcgcggc gccaggcttg gtcctcgacc
      301 tcgatagcgg cctcctgggc caggtcggcg cccaggtcca aggcccattt ggcgaggcgc
      361 acggcgctgg gggagttggc ggcgatctcg gcggccagcc cccgtgccgc ttccggcagc
      421 gccgcgcggg acaccacccg gtcggcgatg ccgagccgca acgcctcggc cgcgtccacc
      481 cgccggccgg tgagcaccag gtccttggcc accgagcgcc ccacgcggcg caccagcagc
      541 tgggtgccac cgccagcggg gaccagcccg aggcgcacct ccggcagccc aaagaccgcg
      601 tcgtcggcag cgaccaccag gtcgcaggca agcgccaact ccatgccgcc gccgagcgcg
      661 aagccgaaca ccgccgcgac cgtcggcagc gggcagcgcc gcagcgcccc gaacgcgcgg
      721 cggaacacct cccgctgctc gaaccagtcg tgatcggtga accctccccg ctgccgcagg
      781 tccgcaccga cacagaaggc ccgctcgcca gcaccttcca gcaccaccgc ccgcaccgac
      841 tgcttgaccg ccagcggctc gaccgcctcg gccagcgcca gcgccagctc ggtcgagatc
      901 gcgttgagcg cctccggccg ctgcaaggtg acggtcgcga tcccctccgc gacctccacc
      961 acgatcgggt ccacccggct cacccgccga cgctaccggc gttgacctgc tggaacgccc
     1021 attcccaggt gcgcgccagg ccatcggcga aggccacgtt gcgctgccag cggagcaccc
     1081 gctcggcctc cccacaatca agcgcgatgc gctgcagctc cccggagcgt ggcggcgcga
     1141 actggggctc cgggccgaac ccggtgacct cgcggagggt ggcgtacagg tccagcaccg
     1201 aggtctccac cccggtgccg atgttgacgc gggcaccgag cgcctcgtcg cgctctccgg
     1261 cgagcaggaa ggcgttcacg acgtcctcca cgtacacgta gtcgcgggtc tgcagcccgt
     1321 cgccgaagat cgtcaccggc tcgccggaca gcagccggtt gcagaagatc gccaccaccc
     1381 cggcctcgcc gtgcgggtcc tggcggggcc cgtagacgtt gccgaggcgg aggctgaccc
     1441 actcgagccc atggatgtcc ctgaagctgg ccaggtagtc ctctgccgag cgcttggcca
     1501 cgccgtacgg gctggtcggc cggggcgggt agtcctctgc gaccggcagg tcgcccgggt
     1561 ccggctcgcc gtaggcggtc ccgcccgacc cggcgaacag cagccgcctg gcgccgttct
     1621 cgaccgccgc ctcggccagc gcgaccgagc cgagcacgtt gacgcgggcg tcgtgggcgg
     1681 ggtcggccac gctgcggcgg acgtcgatct gggcggcgag gtggaacacc acctccgggc
     1741 gggcctcggc gacgatcgcg gccagctcgg gacctgccac gtccgcctcg acgtgctcca
     1801 cccgtccgga gccgaacgcg tccaccaggt tgtcgagctt cccggtggag aggtcgtcga
     1861 cgacggtcac ctcgtgcccg tggtgggcga ggcggtcgac cagggccgag ccgatgaacc
     1921 cggcgccgcc ggttaccagg gtgcgcatgc gtcggccttt cgagcggtcc gggcccggtg
     1981 gccacggccc gggagccccc ggggtccggt cagcgtcggt gagcgccgtc atcctaccgg
     2041 cggtacccgc ccctcgctct tgtcgccggc gctccggtcg tgcagggtac gcgggtgagc
     2101 gagcagatcg aggagcggga cgaggcgccg ccggccgcgc cgcaggcgcg gctgggtgcc
     2161 gccgtccgca ccatgaccag gggctgggcc ctgccctacc tggtcgtcct cgtcgtcgcc
     2221 gccgccgacc tcgtggtcgg cggcgacctc tccatggtgg cgttcctcgt gctcggcccg
     2281 ctgctcgcga gccgcctgtc gaccgaccgg cggcaggtcc tggccgccgg gacggtcgcg
     2341 ttcgcggccg gcgtgctcct cggcgagttc aaccgcgacc tcaccaccgg ccagcaggcg
     2401 ctccggctcc tgctcatcgg cgtgggcacg ctgatcgcgc tcatcaacca gggcgcgctg
     2461 cggcgcgagc agtcggcgct cgagcgggcc gccgacacgg tcgccctggc cgcctcgctg
     2521 gtcgccgggg tcgagcccga ggaggcgtac cggctgctcg cccgctcggc gcgcagcctc
     2581 tacgaggccg acgccgccgc cgtgtaccgg cgggacgccg accagctgct gctcgcggcg
     2641 gacgaccggc acgggctgat cccccccatg ccgggctggc tcggcaggac gaacgtgccc
     2701 gacgccttcg cctccggacc cgtcgaggcg accgtccgct cggccaccgc gcccgaggcg
     2761 accatgctgg cggcgcgcgg gctcggcagc gtgctgtggc tgcccctgct cgacccggcc
     2821 gggcggcaga ccggcgtcat cgcgctcgcc tggcgccggc ccgacccgcg cctgtcggcc
     2881 gaggcgctcg gcgcgtcgcg ccagttcgcc gacctcggcg ggcgggccat ctcgggctcc
     2941 gagcgggtcc gggcccagac cgagatcctc gagcgggtcc aggcactgct gctgtccacc
     3001 ccgcccgcct gggcgcaggg gtaccgcatc ggggtcaggt accagtcggc gagcgggctc
     3061 gcgcagatcg gcggggactt ctacgacgtg gtcgaggtcg aggacggcaa ggggctcgcg
     3121 ttcatcctgg ccgacgc
//
LOCUS       DASPYY010000478         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2047239, whole genome shotgun sequence.
ACCESSION   DASPYY010000478 DASPYY010000000
VERSION     DASPYY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047239"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="aroC"
                     /locus_tag="VEP73_07555"
     CDS             <1..939
                     /gene="aroC"
                     /locus_tag="VEP73_07555"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYT26317.1"
                     /translation="WAEVMAPDPVADPAAAATGRGVPLTRPRPGHADLTGMQKYGFDE
                     ARPVLERASARETAARVAVAAVARALLAELGVSVLSHVVRLGPVEADGAGVPAPGDAA
                     RIDANELRCLDPAAAERMAAAVDQAKAAGDTLGGVFEVVAHGLPPGLGSYVHWDRKLD
                     ARLAAALMSVQAVKGVEVGDGFATAARPGSLAHDEIVPGEPGRPARRTGRSGGIEGGM
                     STGEPLRLRAAMKPLSTLVRALDTVDVATGEPARAVVQRSDVTAVPAAAVVGEAVVAL
                     VLADALLEKTGGDSLDEARRNLDGYLAVLARPRAGR"
     gene            1058..>1581
                     /locus_tag="VEP73_07560"
     CDS             1058..>1581
                     /locus_tag="VEP73_07560"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYT26318.1"
                     /translation="MRPVLPTIVLIGFMGAGKSSVGQLVARALGRELVDTDVVIERTA
                     RMDVAEIFRQEGEDGFRRREAAVIDRAVSAPGRVIAVGGGAVLSADNRTALKQAGLLV
                     YLRATPETLAGRLVGTVDRPLLNTGDRVGRIRDLLVARGPIYETAGDVAIDTDWHSPE
                     QTAVAVLEWYRARI"
BASE COUNT          185 a          615 c          606 g          175 t
ORIGIN      
        1 tgggccgagg tcatggcgcc cgacccggtg gccgaccccg cggcggccgc caccggccgc
       61 ggggtgccgc tcacccggcc ccggccgggc cacgccgacc tgaccggcat gcagaagtac
      121 ggcttcgacg aggcgcggcc ggtgctcgag cgggcgagcg cccgcgagac ggcggcccgg
      181 gtcgcggtcg ccgcggtcgc ccgcgccctg ctcgccgagc tcggcgtcag cgtgctctcc
      241 cacgtcgtcc gcctcggccc ggtcgaggcg gacggggccg gcgtccccgc cccgggcgac
      301 gccgcgcgga tcgacgcgaa cgagctgcgc tgcctcgacc ctgccgccgc cgagcgcatg
      361 gcggcagcgg tcgaccaggc gaaggccgcg ggcgacaccc tcggcggcgt cttcgaggtg
      421 gtggcgcacg ggctcccgcc cgggctcggc tcctacgtcc actgggaccg caagctcgac
      481 gcgcggctgg ccgccgcgct catgagcgtc caggcggtca agggcgtcga ggtcggcgac
      541 gggttcgcga cggccgcccg gcccggctcc ctcgctcacg acgagatcgt ccccggcgag
      601 ccgggccggc ccgcgcgccg caccgggcgc agcggcggga tcgagggcgg catgtccacc
      661 ggcgagccgc tgcgcctccg cgcggccatg aagcccctgt cgacgctggt ccgcgcgctc
      721 gacaccgtcg acgtggccac cggcgagccg gccagggcgg tggtgcagcg gtccgacgtc
      781 accgcggtgc ctgccgccgc cgtcgtcggc gaggcggtgg tcgcgctcgt cctcgccgac
      841 gcgctgctcg agaagaccgg cggcgactcc ctcgacgagg cccgccgcaa cctggacggc
      901 tacctggccg tgctggcccg gccccgagcg ggccgctgag cagggtccag ccggatccgc
      961 gcggcctccg cgagcccgcc ggtcgagaca agcgccgagg ccggctgcta cattgcagcg
     1021 ccaggccgac cgccccggag caaggaggcc ctcgcgcgtg cgaccggtac tgccgacgat
     1081 cgtcctcatc gggttcatgg gggctggcaa gtcatcggtc gggcagctcg tcgcgcgggc
     1141 gctcgggcga gagctggtcg acaccgacgt ggtcatcgag cggaccgcgc gcatggacgt
     1201 ggccgagatc ttccggcagg agggcgagga cggcttccgc cgccgcgagg cggcggtcat
     1261 cgaccgtgcg gtctccgccc ccggccgggt catcgcggtc ggcggcggcg cggtgctctc
     1321 ggccgacaac cgcaccgcgc tcaagcaggc cggcctcctc gtctacctgc gggcgacccc
     1381 cgagacgctc gccgggaggc tggtcggcac ggtggaccgg cccctgctga acaccggcga
     1441 ccgggtgggc aggatccgcg acctcctcgt cgccaggggc cccatctacg agaccgccgg
     1501 ggacgtggcg atcgacaccg actggcacag cccggagcag acggccgtcg cggtgctcga
     1561 gtggtaccgg gcccgcatcg a
//
LOCUS       DASPYY010000479         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1486740, whole genome shotgun sequence.
ACCESSION   DASPYY010000479 DASPYY010000000
VERSION     DASPYY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486740"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1268
                     /locus_tag="VEP73_07565"
     CDS             78..1268
                     /locus_tag="VEP73_07565"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HYT26319.1"
                     /translation="MSSAASSSYDDLDFETRAVHVGSDPDPHTGAVIPPIHQTSTFAQ
                     EGVGGHKGYEYARTGNPTRANLEALLASLEGAAPGAGGALCFASGMAATTTLLQTLRP
                     GDHLVLPSDVYGGTYRVAARLFAEWGLAYSVVDMTDLDAVKQAIRAETRLVWVETPTN
                     PLLGVVDVAAAAELAHTAGALCAVDNTFATPYLQRPLDLGADVVVHSATKYLGGHSDA
                     VSGALVTRLAELYERCRFLQNAAGAVPGPMDCWLVQRGVKTLALRMRAHCHNAMRVAA
                     FLAARPDVAEVRYPGLPGDPGHELAARQMAGFGGMVSFRPAGGVAAARRLVAATRVFT
                     LAESLGGVESLIELPGEMTHASVAGTDAEVPADLVRLSVGIEHPDDLVADLRQAMAAA
                     GTGG"
     gene            1280..1849
                     /locus_tag="VEP73_07570"
     CDS             1280..1849
                     /locus_tag="VEP73_07570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT26320.1"
                     /translation="MRWSEPSPRLAATEGEGAPGGGAAGVVVRPAGQDDLDDLVALYA
                     AVAAEGRWIGGEAPVDVALRRARMAERLDRPDARVLVADDGGRVVGQLGIELAPYGVA
                     DLGMLVADGWRGRGVGSALLRAGIAWARDAGAHKVALQVWPHNQAAIALYEKFGFERE
                     GYLRRHYRRRNGELWDAVVMGLLLVPGRS"
     gene            1934..2389
                     /locus_tag="VEP73_07575"
     CDS             1934..2389
                     /locus_tag="VEP73_07575"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYT26321.1"
                     /translation="MDEASRDQVQAGHVNQAVVVGAPATRVYEYWRDFEHFPQFMEDV
                     ESVTRNPDGSWHWVVGGPLGKRVGWDAVVTEDVPGEVIMWRSVGDSDVQQAGTVRFTD
                     TGGGTELHANIAYTPPAGKVGKLVADVVKDPDKQVARSLERFRALVETW"
     gene            complement(2407..3177)
                     /locus_tag="VEP73_07580"
     CDS             complement(2407..3177)
                     /locus_tag="VEP73_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26322.1"
                     /translation="MLFNFLLNQWLYLAFVRRDAERQEVFVAFAPDENMRIYDRGIRR
                     RLASMVDGDQRRLELAYSLLFTLPGTPVLLYGEEIGMGDDLAVPGRASVRTAMQWTSK
                     EPNGGFSTADPSTYRTPVIGGGPFGWRRCNVADQRRDPGSLLNRVARMIRVHKECPEF
                     GFGAWRLLETGDPAVLGLHFDRQEGGASRASGKGGPGTVTLHNFAAEPRQVTLDLPPD
                     ATIEELLGDEGRERAEADDRTVGLGPYGWRWLRLWPAG"
     gene            complement(3258..3491)
                     /locus_tag="VEP73_07585"
                     /pseudo
     CDS             complement(3258..3491)
                     /locus_tag="VEP73_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          453 a         1262 c         1325 g          463 t
ORIGIN      
        1 ccccgcgccg ccggtcgcgc cgctggtgtt caatggcgtc cgcccggcac agacgcgcga
       61 cgaacggggc caccgacatg tcctccgctg cttcctcctc gtacgacgac ctcgacttcg
      121 agaccagggc cgtgcacgtc ggctcggacc cggacccgca cacgggcgcg gtcatcccgc
      181 ccatccacca gacctcgacc ttcgcgcagg agggggtcgg cggccacaag ggctacgagt
      241 acgcccgcac cggcaacccg acccgggcga acctcgaggc gctgctcgcc tccctcgagg
      301 gcgcggcccc gggcgccggc ggggcgctct gcttcgcgtc gggcatggcc gcaaccacca
      361 cgctgctgca gaccctgcgg ccgggcgacc acctcgtcct gccgagcgac gtgtacgggg
      421 gcacctaccg ggtcgccgcc cggctcttcg ccgagtgggg cctcgcctac agcgtggtcg
      481 acatgaccga cctggacgcg gtcaagcagg cgatccgggc cgagaccagg ctcgtctggg
      541 tggagacgcc gaccaacccg ctgctcggcg tcgtcgacgt cgccgcagcg gccgagctgg
      601 cccacaccgc gggtgcgctc tgcgcggtcg acaacacgtt cgccaccccc tatctccagc
      661 ggcctctcga cctcggtgcc gacgtcgtgg tgcactcggc caccaagtac ctcggcggcc
      721 actcggacgc ggtcagcggc gcgctggtca cccggctcgc cgagctctac gagcggtgca
      781 ggttcctgca gaacgcggcc ggcgcggtgc cagggccgat ggactgctgg ctggtgcagc
      841 ggggggtcaa gacgctcgcg ctgcgcatgc gcgcccactg ccacaacgcg atgcgggtgg
      901 ccgcgttcct ggccgcgcgg cccgacgtgg ccgaggtccg ctatcccggc ctgcccggcg
      961 acccagggca cgagctggcc gcccgccaga tggccgggtt cggcggcatg gtgtcgttcc
     1021 gccccgcggg cggggtcgcg gcggcgcggc ggctggtggc cgcgacgcgg gtcttcaccc
     1081 tggccgagag cctcggaggg gtcgagagcc tgatcgagct ccccggcgag atgacccacg
     1141 cgagcgtcgc cggcaccgac gccgaggtgc cggccgacct cgtccggctc tcggtcggca
     1201 tcgagcatcc cgacgacctg gtcgccgacc tccggcaggc catggccgcg gccggcaccg
     1261 gcggctgagc cgtccggccg tgcgctggtc cgagccgtcc ccacgcctgg ccgcgaccga
     1321 gggagagggc gcgccgggtg ggggggccgc cggcgtggtg gtccggcccg ccggccagga
     1381 cgacctcgac gacctcgtgg cgctctacgc ggcggtcgcc gccgagggac gctggatcgg
     1441 cggcgaggcg cccgtcgacg tcgcgctccg ccgggcgcgc atggccgagc ggctcgaccg
     1501 gccggacgcc cgcgtcctcg tcgccgacga cgggggacgg gtcgtcggcc agctcgggat
     1561 cgagctcgcc ccctacgggg tcgccgacct cgggatgctg gtggcggacg ggtggcgcgg
     1621 gcgcggggtc ggcagcgccc tgctgcgggc ggggatcgcc tgggcgcgcg acgccggcgc
     1681 ccacaaggtc gcgctgcagg tgtggccgca caaccaggcg gccatcgccc tgtacgagaa
     1741 gttcggcttc gagcgcgagg gctacctgcg gcgccactac cgccggcgca acggcgagct
     1801 gtgggacgcc gtggtcatgg gcctgctcct cgtgcccggg cggtcctgag cgggcgccgt
     1861 cgcggcgacc gctggtttgc ccgccgcacc caccggttag gtggcttcct ggaacccgac
     1921 ggaagggagc ggcatggacg aggcgagcag ggaccaggtg caggctggac acgtgaacca
     1981 ggcggtcgtc gtcggggcgc ccgcgacccg ggtgtacgag tactggcgcg acttcgagca
     2041 cttcccgcag ttcatggagg acgtcgagtc ggtgacccgc aaccccgacg gcagctggca
     2101 ctgggtcgtc ggggggccgc tcggcaagcg ggtcgggtgg gacgcggtcg tcaccgagga
     2161 cgtgcccggc gaggtcatca tgtggaggtc ggtcggcgac agcgacgtcc agcaggccgg
     2221 caccgtccgc ttcaccgaca ccggcggcgg cacggagctg cacgcgaaca tcgcctacac
     2281 cccgcctgcc ggcaaggtcg gcaagctcgt cgccgatgtc gtcaaggacc ccgacaagca
     2341 ggtcgcccgc agcctcgagc gcttccgggc gctggtcgag acctggtaga ggcgctggca
     2401 gctgggctac ccggccggcc agaggcggag ccagcgccag ccgtaggggc cgaggccgac
     2461 ggtccggtcg tcggcctcgg cccgctccct gccctcgtcg cccaggagct cctcgatcgt
     2521 cgcgtccggc gggaggtcga gggtgacctg gcggggctcg gcggcgaagt tgtgcagcgt
     2581 cacggtccca ggtccgccct tgcctgaagc gcgcgaggcc ccgccttcct ggcggtcgaa
     2641 gtgcaggccg agcactgccg ggtcgcccgt ctcgaggagc cgccacgccc cgaagccgaa
     2701 ctccgggcac tccttgtgga cccggatcat ccgggccacg cggttgagca gcgagccggg
     2761 gtcgcggcgc tggtcggcga cgttgcaccg ccgccagccg aacgggccgc cgccgatgac
     2821 cggggtccgg tacgtcgacg ggtcggcggt cgagaagccg ccgttcggct ccttcgacgt
     2881 ccactgcatc gcggtgcgca cgctggcccg gccggggacg gcgaggtcgt cgcccatgcc
     2941 gatctcctcg ccgtagagca ggacgggcgt ccccgggagg gtgaacagca ggctgtaggc
     3001 gagctcgagg cggcgctggt ccccgtcgac catggacgcg agccgccgcc ggatgccgcg
     3061 gtcgtagatc cgcatgttct cgtcgggggc gaacgcgacg aagacctcct gccgctcggc
     3121 gtcgcggcgg acgaacgcga ggtagagcca ctggttgagg aggaagttga agagcatgtg
     3181 gagctcgtcg ccgtgctcca caccgccggg ttctcgacgt tcaggtccgg ctggaacttg
     3241 tagaagcggt gcaggtagta ctggccggtg cgctcgtcga gctgccagtt ggagtcctcc
     3301 tcgcccggga agacgatgcc cttcggctcg gggtcgggct cgtcgcgcca gacgtagaag
     3361 tcccggtacg ggttgtcccg cgacgaccgg gcggcccgga accacgggtg ctcgtcggac
     3421 gtgtggttga cgaccaggtc ggtgatcacc ttgatgccgc ggtcgcgggc ggtgcgcagg
     3481 aactcgacca cgtcgccgag ggt
//
LOCUS       DASPYY010000480         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_361359, whole genome shotgun sequence.
ACCESSION   DASPYY010000480 DASPYY010000000
VERSION     DASPYY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361359"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..921
                     /gene="grpE"
                     /locus_tag="VEP73_07590"
     CDS             184..921
                     /gene="grpE"
                     /locus_tag="VEP73_07590"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYT26323.1"
                     /translation="MQKDTKQEVDDVSSAKPAPGEGAGAARTADAAAEAAKDVTAKDV
                     KGPPGNGEVAAAVAAADGAPAAAAEPAAEAASEVPDGAAEGDQSAAEQSKVIEADLAS
                     VRAEAAGYLNDLRRLQAEFDNYRKRMLREQTARVASASQSLVSKLLPVLDNFELAVSH
                     AEQSRDFDRMLKGVEMVFGELQEVLRGEGLSRIEAEGKPFDPERHEAVVAVEQEGAEA
                     GSVVDVVRTGYELQGKVLRPAMVKVAR"
     gene            999..>1585
                     /locus_tag="VEP73_07595"
     CDS             999..>1585
                     /locus_tag="VEP73_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYT26324.1"
                     /translation="MNNPEYFEKDFYAVLGVAKDASPADVKKAYRKLAQKLHPDANPG
                     DKAAEERFKEVGRAYGVLSDPKKRAEYDEARRLLGAGAFGGGLGGGGFPGGFGGGGPR
                     VRVEDLGDLGGLGGLFGNLFGGGRTQQAARRGRDVDAKLTLGFEESVRGGTISLNLTG
                     PSACSTCRGSGARPGTLPKPCSTCNGRGTVTRDQGL"
BASE COUNT          250 a          548 c          616 g          171 t
ORIGIN      
        1 gccgagatca tcgacgaggg cgacgaggag cagaagcaga agggggcctg agccggccga
       61 ggacgaaccc ggagagggga ggccaaccag cctcccctct cccccacggg agcggtgagc
      121 gcgtacgtcg gcacgggtga ccgtccgagg gcgctctcac cggtctctga agggggacaa
      181 cacgtgcaga aggacaccaa gcaggaagtg gacgacgtga gcagcgcgaa gccggctccc
      241 ggcgaggggg ccggcgccgc ccggaccgcg gacgccgccg cagaggcggc caaggacgtc
      301 acggccaagg acgtcaaggg acccccgggc aacggcgagg tcgccgccgc ggtcgccgcc
      361 gccgacggcg cgccggccgc cgccgcggag ccggccgccg aggcagcctc cgaggtcccg
      421 gacggggcgg cagagggcga ccagtcggca gccgagcaga gcaaggtgat cgaggccgac
      481 ctcgccagcg tccgcgccga ggccgctggc tacctgaacg acctgcgccg gctccaggcc
      541 gagttcgaca actaccgcaa gcgcatgctg cgcgagcaga ccgcgcgggt cgcgtccgcg
      601 tcgcagtcgc tcgtgtccaa gctcctgccc gtgctcgaca acttcgagct cgcggtctcg
      661 cacgccgagc agtcgcggga cttcgaccgc atgctcaagg gcgtcgagat ggtcttcggc
      721 gagctgcagg aggtcctgcg gggtgagggg ctctcccgca tcgaggcgga gggcaagccg
      781 ttcgaccccg agcggcacga ggcggtcgtc gccgtcgagc aggagggcgc cgaggccggc
      841 agcgtggtgg acgtcgtgcg tacgggctac gagctccagg ggaaggtgct gcgtcccgcc
      901 atggtgaagg tggccaggta gggccgccgg cgcccgtgcc cccaggcgac gcgggccggg
      961 gcagggcccg gatcccccgg aggggaagag cgaagagcgt gaacaacccg gagtacttcg
     1021 agaaggactt ctacgccgtg ctcggcgtgg ccaaggacgc gtcgcccgcg gacgtcaaga
     1081 aggcgtatcg caagctcgcc cagaagctgc accctgacgc caacccgggc gacaaggcgg
     1141 ccgaagagcg cttcaaggag gtgggcagag cctacggcgt cctgtccgac ccgaagaagc
     1201 gggccgagta cgacgaggcc aggcggctgc tcggcgccgg cgcgttcggc ggcggcctcg
     1261 gcggcggcgg gttcccgggc ggcttcggtg gtggcggccc gcgggtccgg gtcgaggacc
     1321 ttggcgacct gggcgggctc ggcgggctgt tcggcaacct gttcggcggc ggccgcaccc
     1381 agcaggccgc ccgccggggc cgtgacgtcg acgcgaagct caccctcggc ttcgaggagt
     1441 cggtccgcgg tggcaccatc tcgctgaact tgaccggccc gtccgcctgc tcgacctgcc
     1501 gcgggtccgg cgcccggccg gggacgctcc ccaagccttg ttccacgtgt aacggccgcg
     1561 gcacggtcac ccgcgaccag gggct
//
LOCUS       DASPYY010000481         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_503576, whole genome shotgun sequence.
ACCESSION   DASPYY010000481 DASPYY010000000
VERSION     DASPYY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503576"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEP73_07600"
     CDS             <1..318
                     /locus_tag="VEP73_07600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26325.1"
                     /translation="VVPEKLFGYLAAGLPVVTTAVAGAQDLRRLVWIARDPSEFARAI
                     QGALKRDRERRRTLRQAAVAPLSWDAVAEHVVASVDAVRAGAELPGPPELKVPAIPGF
                     LAR"
     gene            396..1226
                     /locus_tag="VEP73_07605"
     CDS             396..1226
                     /locus_tag="VEP73_07605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYT26326.1"
                     /translation="MPDDQVPDDRPEWLTRRAPEPDGASAPHHAPEPDLAPARGVPHP
                     GRPVVTLALLAVNAVMLLVEAASSGGVAGLLEPTNQALCRLGALNAAAIAESGQYWRL
                     FTVMVLHAGVIHFALNSYALYIFGPQLEYLLGRARYLAIYVGAGFVGAAASFAFGRSV
                     LGVGASGAIFGLLGAMVAFFFRRRRIIGTAPLRNVLFIVAINLFLATQIANIDNTAHI
                     GGFAGGLVGMALFDAVPADRGRLRNLALAAPFLVGIALTAYGVATFPAGTGQLTCAGV
                     "
     gene            complement(1207..1941)
                     /locus_tag="VEP73_07610"
     CDS             complement(1207..1941)
                     /locus_tag="VEP73_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26327.1"
                     /translation="MLASVLRDAARQYRRRPLAAAPSLVVGGASVALQWAPGPVRDPG
                     AIVVVVVGVLAELFQIAYLAGALASGGSGAVEPGGGGAAAAVAAARRSAGPGLRAALL
                     LFAYLAIAYMVALLLLGTRDVTTLTGRQETAFQVGVGPLLAVAFAFLAVLSQGIVLGG
                     ERRARLAAVASHRVAAVHFPICLVIGLVQALAWVLGGFSPGVAAQLAITVCLGLVDPL
                     RIAASNSLFLATRSLHAAEPVRPPRR"
     gene            2057..3136
                     /gene="selD"
                     /locus_tag="VEP73_07615"
     CDS             2057..3136
                     /gene="selD"
                     /locus_tag="VEP73_07615"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYT26328.1"
                     /translation="MPPPPRTEAVRLTSLSHGAGCACKLPLAALDELMLAVGPGLGAA
                     AGDLLVGAGEGDDAAVYRLDGSRALVVTTDFFTPIVDDARDWGRVAAANALSDVYAMG
                     GRPLVALNLTAWPAASLPLELLAEVLRGGAEVAAEAGCLVVGGHSVDDPEPKYGMAVV
                     GLADPDRLLTLDRARPGDHLVLTKPLGTGVVATALKRGGADPGSVASAVASMTALNAA
                     ASEAALAAGVRAATDVTGYGLLGHLHRMLRASGAAGEVDAARVPFLPGAAELAGAGFV
                     SGGTRSNQAFLAGRVALDPGVPPVVATLLHDAQTSGGLLLAVPPGVLAGLVEDLHGRG
                     LRPALVGRVLGGPAGAIRVRRLPPG"
     gene            3369..3788
                     /locus_tag="VEP73_07620"
     CDS             3369..3788
                     /locus_tag="VEP73_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HYT26329.1"
                     /translation="MFAIDRQSYDALVAHARAEYPNEACALLAGNGRVERVYALPNAE
                     ASPTFYVVEPKAQLQAMNEMDDLGWDLVGIFHSHTFTEAYPSRTDVELAAYPDAAYLI
                     LSLADPDAPELRAYRIADGEVAEEPVEVVAGGPAVAG"
     gene            3994..4308
                     /locus_tag="VEP73_07625"
     CDS             3994..4308
                     /locus_tag="VEP73_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYT26330.1"
                     /translation="MPVEVRIPTILRKYSDGAKSVAAEGDTVRSLLADLDARYPGIAS
                     QLLNEDGSLHRFVNVYVNDEDVRFLGALDAKLEEGDVVAILPAVAGGSGGLMVSPPAD
                     GG"
     gene            4312..>5055
                     /locus_tag="VEP73_07630"
     CDS             4312..>5055
                     /locus_tag="VEP73_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT26331.1"
                     /translation="MRYEDLVDGIGNTPLVGLPRLSPRPDVRLWAKLEGQNPTGSVKD
                     RIAKAMVVDAEKTGRLTPGATLLEPSSGNTGIALALVARLRGYRLVVVMPENTSIERR
                     QLLELYGATVVPSEAAKGSNGSIELAKELAAANPDWVMLYQYGNPANALAHYETTGPE
                     IWRDLPEVTHFVAGLGTTGTLMGVGRYLKEQDAGIQIVAAEPEYGDLVYGLRNLDEGF
                     VPPIFDETLLDRRIKVNSHDALRRTRELAA"
BASE COUNT          651 a         1983 c         1827 g          594 t
ORIGIN      
        1 gtggtgcccg agaagctgtt cggctacctc gcggccggcc tgcccgtggt caccaccgcg
       61 gtcgccggcg cgcaggacct gcgccggctc gtgtggatcg cccgcgaccc gtcggagttc
      121 gcccgcgcca tccagggtgc gctcaagcgg gaccgggaac ggcgccggac gctccgccag
      181 gccgccgtcg ccccgctgtc ctgggacgcg gtggccgagc acgtcgtcgc gagcgtcgac
      241 gcggtgcggg ccggcgccga gctccccggc ccgcccgagc tgaaggtccc ggccatcccg
      301 ggcttcctcg cccgctgaca cggcgcccgc ggagcgccgg ccgaacccac ccgcggatgg
      361 catgctggac ggcaggatct cgagaggagc acccgttgcc cgacgaccag gtgccggacg
      421 accgcccgga atggctgacc cgccgggcgc cggagcccga cggcgcgtca gcgccccacc
      481 acgcgccgga gccggacctc gcgccggcgc ggggcgtgcc ccaccccggc cgcccggtcg
      541 tcaccctcgc gctgctggcc gtgaacgccg tcatgctgct cgtcgaggcc gcctcgagcg
      601 gcggcgtggc cgggctgctc gagccgacga accaggcgct gtgccgtctc ggcgcgctca
      661 acgcggccgc gatcgccgag tccgggcagt actggcgcct gttcaccgtg atggtgctgc
      721 acgccggcgt gatccacttc gcgctgaaca gctacgccct gtacatcttc gggccgcagc
      781 tcgagtacct gctcggccgg gcccgctacc tcgccatcta cgtcggggcg ggcttcgtcg
      841 gggcggcggc cagcttcgcc ttcgggcggt cggtgctcgg ggtgggcgct tccggcgcga
      901 tcttcggcct gctcggggcc atggtcgcct tcttcttcag acgccgccgg atcatcggca
      961 ccgcgcccct gcggaacgtg ctgttcatcg tcgcgatcaa cctgttcctg gccacccaga
     1021 tcgccaacat cgacaacacg gcgcacatcg gggggttcgc gggcggcctc gtcggcatgg
     1081 ccctgttcga cgccgtccca gccgaccgcg ggcgcctgcg caacctcgca ctggccgcgc
     1141 ccttcctggt cggcatcgcg ctcaccgcct acggggtggc caccttcccg gccggcaccg
     1201 gccagctcac ctgcgcgggg gtctgacggg ctctgccgcg tgcagcgagc gggtggcgag
     1261 gaacagcgag ttgctcgccg cgatccggag cgggtcgacg agcccgaggc agaccgtgat
     1321 cgccagctgc gccgcgacgc cgggcgagaa ccccccgagg acccaggcga gcgcctggac
     1381 cagcccgatg accaggcaga tcgggaagtg gaccgccgcg acgcggtggg acgcgaccgc
     1441 ggccagccgc gcgcggcgct cgccgccgag gacgatcccc tgggacagca cggccaggaa
     1501 ggcgaaggcg accgcgagca gcggcccgac gccgacctgg aacgccgtct cctggcggcc
     1561 cgtgagcgtg gtgacgtccc gcgtcccgag cagcagcagg gcgaccatgt acgcgatggc
     1621 gaggtaggcg aacaggagca gggcggcccg caggccgggc ccggccgacc ggcgcgccgc
     1681 tgccacggcc gcggcggcgc cgccgccgcc gggctcgacc gcgccgctgc cgccggacgc
     1741 cagcgcgccg gccaggtagg cgatctggaa cagctcggcg agcacgccca ccacgaccac
     1801 aacgatggca ccggggtccc ggaccgggcc cggggcccac tgcagcgcca ccgacgcgcc
     1861 accgaccacg aggctgggcg cggcggcgag cggacggcgc cggtactggc gggcggcgtc
     1921 gcggaggacg gaagcgagca acggggtctc cgaagggggt ccggcacctt tgcatggaca
     1981 gacgtgggcg gcggccctgt cccgggcggc cctatcctaa gggaggacgc aacccgcccc
     2041 cccgaacgga gctcccatgc cgccaccacc gcggaccgag gccgtccggc tgacctcgct
     2101 gtcccacggc gccgggtgcg cctgcaagct tcccctcgcg gcgctcgacg agctcatgct
     2161 ggcggtcggc cccggtctcg gcgcggcagc gggcgacctg ctggtcggcg ccggcgaggg
     2221 cgacgacgcg gccgtgtacc gcctcgacgg gtcgagggcg ctggtcgtca ccaccgactt
     2281 cttcaccccg atcgtcgacg acgcgcggga ctgggggcgg gtcgcggccg ccaacgccct
     2341 gtccgacgtc tacgccatgg gcgggcggcc gctggtcgcg ctcaacctca ccgcctggcc
     2401 ggccgcgagc ctccccctcg agctgctcgc cgaggtgctg cggggcggcg cggaggtcgc
     2461 cgccgaggcg ggctgcctgg tcgtcggcgg ccactcggtc gacgacccgg agccgaagta
     2521 cggcatggcc gtcgtcgggc tggccgaccc ggaccggctc ctcaccctcg accgggcccg
     2581 gcccggcgac cacctcgtcc tcaccaagcc gctcgggacc ggcgtggtcg ccaccgcgct
     2641 caagcgcggc ggcgccgacc ccggctcggt cgcgagcgcg gtcgcctcca tgacggccct
     2701 gaacgcggcc gcctcggagg ccgccctggc cgccggggtc cgggcggcca ccgacgtgac
     2761 cggctacggg ctgctcggcc acctgcaccg catgctgcgg gcgagcggcg cggccggcga
     2821 ggtggacgcg gcgcgggtgc cgttcctccc cggcgccgcc gagctggccg gggccggctt
     2881 cgtgtccggc gggaccagga gcaaccaggc gttcctcgcc ggcagggtcg cgctcgaccc
     2941 cggggtcccg ccggtggtcg ccaccctgct ccacgacgcc cagacgtccg gcggcctgct
     3001 cctcgcggtc ccgcccggcg tcctggccgg cctcgtcgag gacctgcacg gccgcggcct
     3061 gcgccccgcc ctggtcgggc gggtgctggg cggaccggcg ggcgcgatcc gcgtgcgccg
     3121 cctgccgccc ggctgacccg cgggcggacc accacccgcc cgaccagggg cggcgggccg
     3181 tggcgcggtc acggcggcga cctgccacgc ggagaccgcc atggtcgctc ccaggccgcg
     3241 atgctaggct cgtgaccggg gcagcggacc accactcgcc gaccagtccg gacgccggaa
     3301 cagcagcgac cccagggtgg ttggaccggg tggagggacc gacgagttgg gacgagttgg
     3361 aaggaccgat gttcgcgatc gaccggcaga gctatgacgc ccttgtcgcc cacgcccggg
     3421 cggagtaccc caacgaggcg tgcgcgctgc tggccgggaa cggccgcgtg gagagggtct
     3481 acgccctgcc gaacgcggag gccagcccga ccttctacgt ggtggagccc aaggcccagc
     3541 tccaggcgat gaacgagatg gacgacctcg gctgggacct ggtcgggatc ttccactccc
     3601 acaccttcac cgaggcctac ccgtcccgca ccgacgtcga gctggccgcc taccccgacg
     3661 ccgcgtacct catcctgtcc ctcgccgacc cggacgcgcc cgagctgcgc gcgtaccgca
     3721 tcgcggacgg cgaggtcgcc gaggagccgg tcgaggtcgt ggccggcggc cccgcggtgg
     3781 ccgggtgagg cccgtgcgcc gagcgctccc cctgtgcggg cccgccaccg ggctcgcctc
     3841 gccggtctgg caccgccagg gaccggccgc ccgctacggg cgatgtcggc cctggccgac
     3901 gcacggcgcc tgagccgcgt cccggctcgc ccgaccgccg tcagcgcgac cggccgccgc
     3961 ccgtcgccgc atccgaccgc caggagagct tccatgcccg tcgaagtccg catccccacg
     4021 atcctccgca agtactcgga cggcgcgaag agcgtcgccg ccgagggcga caccgtccgc
     4081 agcctgctcg ccgacctgga cgcccgttac ccgggcatcg cgagccagct gctcaacgag
     4141 gacggctcgc tgcaccgctt cgtgaacgtc tacgtcaacg acgaggacgt ccgcttcctc
     4201 ggcgcgctcg acgccaagct cgaggagggc gacgtcgtcg ccatcctccc cgccgtagcc
     4261 ggggggtccg gggggctcat ggtcagcccc ccggccgacg gggggtgagg catgcggtac
     4321 gaggacctgg tcgacgggat cggcaacacg ccgctcgtcg gcctgccccg cctgtcgccc
     4381 cgcccggacg tccgcctctg ggccaagctc gaggggcaga acccgaccgg cagcgtcaag
     4441 gaccgcatcg ccaaggcgat ggtcgtcgac gccgagaaga ccggccggct caccccgggc
     4501 gcgaccctgc tcgagccctc cagtggcaac accggcatcg ccctcgcgct cgtcgcccgc
     4561 ttgcgcgggt accggctcgt ggtcgtcatg ccggagaaca cctcgatcga gcgcaggcag
     4621 ctccttgagc tgtacggggc cacggtggtg ccctcggagg ccgccaaggg gtccaacggc
     4681 tcgatcgagc tggccaagga gctggccgcc gccaaccccg actgggtcat gctctaccag
     4741 tacggcaacc cggccaacgc gctcgcgcac tacgagacca ccggccccga gatctggcgc
     4801 gacctgcccg aggtgacgca cttcgtcgcc gggctcggca ccaccggcac cctcatgggg
     4861 gtcggccgct acctgaagga gcaggacgcg gggatccaga tcgtcgccgc cgagccggag
     4921 tacggcgacc tcgtctacgg gctccgcaac ctcgacgagg gcttcgtccc cccgatcttc
     4981 gacgagacgc tgctcgaccg gcgcatcaag gtgaactcgc acgacgccct gcgccgcacc
     5041 cgcgagctgg ccgcc
//
LOCUS       DASPYY010000482         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_782911, whole genome shotgun sequence.
ACCESSION   DASPYY010000482 DASPYY010000000
VERSION     DASPYY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782911"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VEP73_07635"
     CDS             complement(<1..702)
                     /locus_tag="VEP73_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYT26332.1"
                     /translation="MPKTYDPTTVEEPTYQRWLDEGLFRADARSGRPRWSMALPPPNV
                     TGSLHIGHALDHTFQDYLARFHRMLGYEVLWLPGMDHAGIATQNVVERELAKEGKTRF
                     DLGREAFIERVWSWKAESGGRILGQMRRLGDSVDWSRERFTMDDGLSRAVREVFVSLY
                     EQGLLYRGQRIINWCPRCLTALSDIEVEHEEVPGELAHLRYPLADGSGSVGVATSRAE
                     TMLGDTAVAVHPDDER"
     gene            complement(1010..1426)
                     /locus_tag="VEP73_07640"
     CDS             complement(1010..1426)
                     /locus_tag="VEP73_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26333.1"
                     /translation="MAGRPRPLARQLGMGALRVAELAVVVAGGCLAVSVLLSWVLGRG
                     FGRTAALVCALVGAGMMFGGSWRNAPVGVLDRRRRRLLDERRRQDERDPAARARREVE
                     EREPPSVVSPGGVASLAGIVVIAAGFAADALLGHGR"
     gene            complement(1441..>2017)
                     /locus_tag="VEP73_07645"
     CDS             complement(1441..>2017)
                     /locus_tag="VEP73_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT26334.1"
                     /translation="IDTTNVLFICGGAFAGLEKIIESRIGRKGVGFGADLRRIGDKDV
                     GEILAKVLPEDLLKFGLIPEFIGRLPVISSVHSLDKAALVRILVEPKNALVKQYRKFF
                     DFDGVELEFSDDALQAVADQAILRGTGARGLRAILEEVLLDVQYELPSRTDVRRCVIT
                     ADVVLRGVNPTLVPRDREAPAGERARRERSA"
BASE COUNT          278 a          727 c          753 g          259 t
ORIGIN      
        1 gcgctcgtcg tcggggtgca cggccaccgc ggtgtcgccg agcatggtct cggcccggga
       61 ggtggccacg ccgacggatc cggagccgtc ggcgagcggg tagcggaggt gggccagctc
      121 gccgggcacc tcctcgtgct ccacctcgat gtcggacagg gcggtgaggc agcgcgggca
      181 ccagttgatg atgcgctggc cccggtagag caggccctgc tcgtacaggc tcacgaacac
      241 ctcgcggacc gcccgcgaga gcccgtcgtc catcgtgaag cgctcgcgcg accagtcgac
      301 cgagtcgccg agccggcgca tctggccgag gatgcgcccg cccgactcgg ccttccagct
      361 ccacacccgc tcgatgaacg cctcgcggcc gaggtcgaag cgggtcttgc cctccttggc
      421 gagctcgcgc tcgaccacgt tctgggtggc gatgccggca tggtccatgc cgggcagcca
      481 gagcacctcg tacccgagca tccggtggaa gcgggccagg tagtcctgga aggtgtggtc
      541 gagggcgtgg cctatgtgca gggagccagt cacgttgggc gggggcagcg ccatgctcca
      601 gcgcggcctg cccgaccggg cgtcggcgcg gaacaggccc tcgtccagcc agcgctggta
      661 ggtgggttcc tcgaccgtcg tggggtcgta ggtcttgggc agctcgcgcc gccctgcggc
      721 gccggcggcc tgggtgtgcg cctgccgggc gcctgtcgat tcggtcacgg gaaatgctcc
      781 tcagcgtcgt cggcccggtg tggccgggcg gagcgggaaa cgccgatacg gcgggaaggg
      841 cgggcgcggg cgggcgcccg aggcccggac ggcgggccta ggcgggtact cgcagggcgg
      901 aggcagtctg cgctgccagc gtcgccgcga acctgctcac cgtacgaccg tcctctcgaa
      961 gcttgccgag ccacaagcgt acgggttccg tgccgtcccg ctcaactcgt caccgcccgt
     1021 ggccgagcag ggcgtcagcc gcgaagccgg cggcgatgac gacgatgccg gcgaggctcg
     1081 cgaccccgcc gggggagacg accgagggag gctcgcgctc ctccacctcg cggcgcgccc
     1141 gggcggccgg gtcgcgctcg tcctgccggc ggcgctcgtc gaggaggcgc cggcggcgcc
     1201 ggtcgagcac gccgacgggc gcgttgcgcc aggagccgcc gaacatcatc cccgcgccca
     1261 ccagcgcgca cacgagtgcg gcggtgcgcc cgaagccgcg cccgaggacc cagctcaaga
     1321 ggaccgacac ggccaggcag ccacccgcga cgacgacggc cagctcggcg acccgcagcg
     1381 ctcccatgcc gagctggcgg gcgagcgggc ggggccggcc ggccatcggc ggcgctcagg
     1441 tcaggccgag cgctccctgc gggcgcgctc ccccgccggg gcctcgcggt cccggggcac
     1501 cagcgtcggg ttcacgcccc gcaggaccac gtcggcggtg atcacgcagc ggcgcacgtc
     1561 ggtccgcgag ggcagctcgt actgcacgtc gagcaggacc tcttccagga tcgcccgcag
     1621 gccgcgcgcg cccgtccccc gcaggatcgc ctggtcggcc actgcctgga gcgcgtcgtc
     1681 ggagaactcg agctcgacgc cgtcgaagtc gaagaacttg cggtactgct tgacgagcgc
     1741 gttcttgggc tcgaccagga tccgcaccag cgcggccttg tcgagggagt ggaccgacga
     1801 gatgaccggc agccgcccga tgaactccgg gatgaggccg aacttgagca ggtcctcggg
     1861 caggaccttg gccaggatct caccgacgtc cttgtcgccg atgcggcgga ggtcggcgcc
     1921 gaagccgacc cccttgcggc cgatccgcga ctcgatgatc ttctccagcc ccgcgaaggc
     1981 gccaccgcag atgaacagca cgttggtggt gtcgatc
//
LOCUS       DASPYY010000483         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_80116, whole genome shotgun sequence.
ACCESSION   DASPYY010000483 DASPYY010000000
VERSION     DASPYY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80116"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="aat"
                     /locus_tag="VEP73_07650"
     CDS             <1..873
                     /gene="aat"
                     /locus_tag="VEP73_07650"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HYT26335.1"
                     /translation="AAIHNAMGLVWPSAYARAAGQAPVSLEAGAQRREPVDPTSDRGA
                     RVSSPGIDPGRDPGRAPSGVGASRWRFPDPAGAAPEGVVGFGADLEPATLVHAYRHGI
                     FPWPHPGAPLPWFSPDPRGVLPLDGLAVSRSLRQRLRRCGWHTTVDQAFDAVIAACAD
                     RGAAGTWITAPMRAAYRRLHRLGWAHSLEVWADGVLAGGLYGIGIGGVFTGESMFHRA
                     ADASKVALVDLVARLAEAGGSLVDVQVLTPHLERLGALEVQRSDYLALLHRVRDDPVR
                     LPEARRPVAALVDG"
     gene            910..>1613
                     /locus_tag="VEP73_07655"
     CDS             910..>1613
                     /locus_tag="VEP73_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYT26336.1"
                     /translation="MQGTMTAKILEAHLAGGRLAPGEEVAVTIDQTLLQDATGTMACM
                     EFEALGFDEVKVPTAMQYIDHNMLQFDHRNADDHLFLQTFAARYGIRFSRAGNGICHQ
                     VHTERVARPGATLLGADSHTPTSGAVGSIAIGAGGLEVALAMAGRPLRMPTPVVVGVH
                     LDGELPPWVASKDLILWLIRRYTVKGGLNRIFEFIGPGVAGLPVTRRATVCNMITELG
                     GTTAIFPSDEQTRRFLR"
BASE COUNT          202 a          627 c          575 g          209 t
ORIGIN      
        1 gccgcgatcc acaacgcgat ggggctcgtc tggccgtccg cctacgcccg ggcggcaggg
       61 caggcgccgg tatcgttgga agccggcgcg cagcgccgcg aaccggtgga cccgacgagc
      121 gaccgcggag cacgagtgag cagtccaggc attgacccgg gcagggaccc cgggcgcgcg
      181 ccgtcggggg tgggggcgtc ccgctggcgg ttccccgacc cggccggcgc cgcgcccgag
      241 ggcgtcgtgg ggttcggcgc cgacctcgag ccggcgaccc tcgtccacgc ctaccggcac
      301 ggcatcttcc cgtggcccca cccgggcgcc ccgctgccgt ggttctcgcc cgacccccgg
      361 ggcgtgctgc cgctcgacgg cctcgcggtg tcccgctcgc tccgccagcg cctgcgccgc
      421 tgcggctggc acaccaccgt cgaccaggcc ttcgacgcgg tgatcgccgc gtgcgccgac
      481 cggggagccg ccgggacctg gatcaccgcc ccgatgcggg ccgcgtaccg ccgcctgcac
      541 cggctgggct gggcccacag cctcgaggtg tgggccgacg gggtgctcgc cggcggcctg
      601 tacggcatcg ggatcggggg cgtgttcacc ggcgagtcga tgttccaccg ggccgccgac
      661 gcctccaagg tcgccctggt cgacctggtc gcccgcctcg ccgaggccgg cggcagcctc
      721 gtcgacgtcc aggtcctcac cccgcacctc gagcgcctcg gggcgctcga ggtccagagg
      781 agcgactacc tggccctgct gcaccgggtg cgggacgacc cggtccgcct gcccgaggcc
      841 cggcgaccgg ttgccgcgct ggtggacggg taggatcgcc gcggtcgcct cgacctcagg
      901 agggcaagca tgcagggcac gatgaccgcg aagatcctcg aggcccacct ggccggtggg
      961 cgcctcgccc cgggcgagga ggtcgccgtc acgatcgacc agacgctgct gcaggacgcc
     1021 accggcacga tggcgtgcat ggagttcgag gcgctcgggt tcgacgaggt caaggtcccg
     1081 accgccatgc agtacatcga ccacaacatg ctgcagttcg accaccgcaa cgccgacgac
     1141 cacctgttcc tgcagacctt cgcggccagg tacggcatcc ggttctcccg ggccggcaac
     1201 ggcatctgcc accaggtcca caccgagcgg gtcgcccgcc cgggggcgac cctgctcggc
     1261 gccgactccc acacgccgac ctccggggcg gtcggctcga tcgccatcgg cgcgggcggt
     1321 ctcgaggtgg cgctggcaat ggccggccgg ccgctccgca tgcccacgcc ggtcgtcgtc
     1381 ggcgtccacc tcgacggcga gctgccgccg tgggtcgcgt ccaaggacct catcctctgg
     1441 ctgatccgca ggtacacggt caaggggggc ctgaaccgca tcttcgagtt catcgggccc
     1501 ggcgtcgccg gcctgcccgt cacccggcgg gcgaccgtct gcaacatgat caccgagctc
     1561 ggcgggacga ccgcgatctt cccgtccgac gagcagaccc gccgcttcct gcg
//
LOCUS       DASPYY010000484         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2188454, whole genome shotgun sequence.
ACCESSION   DASPYY010000484 DASPYY010000000
VERSION     DASPYY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188454"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEP73_07660"
     CDS             <1..744
                     /locus_tag="VEP73_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26337.1"
                     /translation="AVEPAVPPTSRDAAYWAKGVSRLELGAVPAEAVNLNVSGRRVVG
                     PVQGFGKMWQKTYRVELPGAAATPAEVITVWKRDFQSFWPKGNRFYGPLTGIAPGEVA
                     LLNLDMAGRLRLSTGVLVLYADDESFTFMTPQGHMFASWITFSAAAQEDCPVVVQAQI
                     LMRAGDPVYELGLALGGHRQEDRFWQHTLASVATRFGAEDPVVQTTVVCVDRRRQWAN
                     ARNVWHNAAIRSGLWVLATPFRLAARPLR"
     gene            complement(792..2417)
                     /locus_tag="VEP73_07665"
     CDS             complement(792..2417)
                     /locus_tag="VEP73_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYT26338.1"
                     /translation="MADTVDRLARRYLDALFEVSPITATFFGVHAHDDRLDELDAARR
                     DAVSRRLSELRAEVTAVVPTDAEEAADRDALAAAIAGYLFVEEVERPWHRNPFEAATA
                     IPSAVLLLVAREFAPLEERLGSVVSRLEAAPRFLAQARELLDEPCPALWRGMATGAAR
                     GGAGFLAEALGPLAAGTRLAGRVEAAAEKATAALEEFAGWLEAEHAARFPGDAPFAIG
                     EQALARKLREAHCFDTPPAGFVAIGEQQLSVLDDALAEQARRLGSDDWPAKLDEVKGR
                     HPSQEELLPAYRRELDRLEQFVFEHDLATNPEARALVEATPGFLRPVMGYAAYLQAGP
                     FDAYQQGYFWVTPPPEDAGLRDHSWASIPAIAAHEGYPGHHLQISSVNRLESLTRRAV
                     RSPVMQEGWGLYTEQLMADVGYYGDEARLAQLSMRLFRALRIVLDMRLSAGELGYEAA
                     VERAVAIGRLAQPTARSEVARYTMSPTQPFSYLVGCLELERLRAESQARLGDAFRLRR
                     FHDRVLSYGHMPPALVARAMAADDEDERRRLAE"
     gene            complement(2483..4231)
                     /locus_tag="VEP73_07670"
     CDS             complement(2483..4231)
                     /locus_tag="VEP73_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26339.1"
                     /translation="MAVASAAVLIQTAAGLSMPYLVKVAIDRGVVPRRLEVVDRVAAA
                     YVAIALVQLVGGRVETLTVAAVGQRVLFAVRRRLFGHLQTLSLDFYERERTGRLVARM
                     TNDVEAMSDLVTDGLVNLVTSLVTLVGIAAILVALDWRLALATLAVAPLVGLAAAWFR
                     RWSSTAYRQVRETASVVTVHAQEALAGVRAIQAFRREGATLDRLAGANDAERRAHRWT
                     IALASFFFPGIEFVGTAATVVVLGVGGAQVLGGRMQIGTLAAFLLYLRSLFDPVQQLS
                     ELYDTFQAATAGAERIGAVLAEEPTVREAAEPVPLPAPDGEVRLEGVHFSYAAPGADV
                     RGPVADGGGPAVGRAAVPPPGEVLHGVDLHVPAGTTVALVGPTGAGKSTVAKLIARFY
                     DPQAGRVTLDGVDLRAVRLADLRTAMGYVPQEGFLFSGTVADNIRFGRPDATRAEVEA
                     AARAVGADTVIEGLPAGFDTEVGERGALLSAGERQLIAFARAWIADPALLVLDEATSH
                     LDVVTEARVQQALRRLRQGRTTVLIAHRLSTVAEADQVAVIEDGRVVEAGSPAHLLTA
                     GGRFADLYGRWLAGAA"
     gene            complement(4329..>4543)
                     /locus_tag="VEP73_07675"
     CDS             complement(4329..>4543)
                     /locus_tag="VEP73_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26340.1"
                     /translation="TVLLVANRRASLRLADRIVLLDRGRVVAVGRHDQLAGREPRYRA
                     VLAHSNAGVDRLVAEGAATEGRAARV"
BASE COUNT          599 a         1708 c         1704 g          532 t
ORIGIN      
        1 gccgtcgagc cggccgtgcc cccgacctca cgcgacgccg cctactgggc caagggggtg
       61 tcgaggctcg agctcggggc cgtcccggcc gaggccgtca acctgaacgt cagcggccgc
      121 cgcgtcgtgg gcccggtcca gggcttcggc aagatgtggc agaagaccta ccgggtggag
      181 cttcccggtg ccgccgcgac cccggcggag gtcatcaccg tctggaagcg ggacttccag
      241 tccttctggc ccaagggaaa ccggttctac gggccactga ccggcatcgc cccgggcgag
      301 gtcgcgctgc tgaacctcga catggccggg cggctgcggc tctcgaccgg cgtgctcgtc
      361 ctctacgccg acgacgagtc cttcacgttc atgacgccgc aggggcacat gttcgcgagc
      421 tggatcacct tcagcgcggc cgcgcaggag gactgcccgg tggtcgtgca ggcccagatc
      481 ctgatgcgcg cgggcgaccc ggtctacgag ctcggcctcg ccctcggcgg ccaccgccag
      541 gaggaccggt tctggcagca caccctcgcg tcggtggcga cccggttcgg ggccgaggac
      601 ccggtcgtcc agaccacggt ggtctgcgtc gaccgccggc ggcagtgggc gaacgcccgc
      661 aacgtctggc acaacgccgc catccgctcc ggcctctggg tcctcgccac accgttccgc
      721 ctggccgccc gccccctcag gtgacggccc gacgcccacc cacgattgca gggccgggct
      781 gggcctgagc gtcattcagc caggcggcgc cgctcgtcct cgtcgtcggc ggccatcgcg
      841 cgggcgacga gggccggggg catgtgcccg taggagagca cccggtcgtg gaagcggcgg
      901 agccggaacg cgtcccccag gcgcgcctgc gactcggccc gcaggcgctc gagctcgagg
      961 cagccgacga ggtagctgaa cggctgggtc gggctcatcg tgtagcgcgc gacctccgag
     1021 cgcgcggtgg gctgggccag ccggccgatg gcgacagccc gctccacggc cgcctcgtag
     1081 ccgagctccc cggccgagag ccgcatgtcg agcacgatgc ggagggcccg gaacaggcgc
     1141 atggagagct gggcgagccg tgcctcgtcg ccgtagtacc cgacgtccgc catgagctgc
     1201 tcggtgtaca gcccccagcc ctcctgcatg accggggagc ggaccgcgcg cctggtcagc
     1261 gactcgagcc ggttgacgct cgagatctgc aggtggtggc ctgggtagcc ctcgtgggcg
     1321 gcgatggccg ggatgctcgc ccagctgtgg tcgcggagcc cggcgtcctc cggtggcggc
     1381 gtcacccaga agtagccctg ctggtaggcg tcgaacgggc cggcctggag gtaggcggcg
     1441 tagcccatga ccggacgcag gaacccgggc gtcgcctcga ccagcgcgcg cgcctcgggg
     1501 ttggtggcca ggtcgtgctc gaacacgaac tgctcgagcc ggtcgagctc cctgcggtag
     1561 gcgggcagca gctcctcctg cgacgggtga cggcccttca cctcgtccag cttggccggc
     1621 cagtcgtccg acccgagccg tcgagcctgc tcggcgaggg cgtcgtcgag taccgagagc
     1681 tgctgctcgc cgatcgcgac gaacccggcc ggtggcgtgt cgaagcagtg cgcctcgcgc
     1741 agcttgcgcg cgagggcctg ctcgccgatg gcgaaggggg cgtcgccggg gaacctggcc
     1801 gcgtgctcgg cctcgagcca gccggcgaac tcctcgaggg ccgcggtggc cttctcggcc
     1861 gccgcctcca cccggccggc gagcctggtg cccgcggcga gggggccgag ggcctcggcg
     1921 aggaagccgg cgccgccccg ggccgccccg gtggccatcc cccgccacag cgccgggcag
     1981 ggctcgtcga gcagctccct tgcctgggcg aggaaccgcg gcgcggcctc caggcggctg
     2041 acgacgctgc cgagccgctc ctccagcggc gcgaactcgc gggcgacgag cagcaggacc
     2101 gcgctcggga tggcggtcgc cgcctcgaac gggttgcggt gccagggccg ctcgacctcc
     2161 tcgacgaaca ggtacccggc gatcgcggcg gccagggcgt cacggtcggc cgcttcctcg
     2221 gcgtcggtcg ggacgacggc ggtgacctcg gcccggagct cggagagccg gcgcgagacg
     2281 gcgtcccgcc gggcggcgtc gagctcgtcg aggcggtcgt cgtgggcgtg cacgccgaag
     2341 aacgtggccg tgatcggcga gacctcgaag agggcgtcga ggtagcggcg ggcgaggcgg
     2401 tcgaccgtgt cggccaaggg gcacgctcct tcacgctgcc cgaggtcagg gacgcgacca
     2461 gggtagccgc tccggtggcg gctcaggccg ccccggccag ccagcggccg tagaggtcgg
     2521 cgaagcgacc tccggcggtc agcagatggg cgggagagcc ggcctcgacc acccggccgt
     2581 cctcgatcac ggcgacctgg tcggcctcgg cgaccgtgga gagccgatgg gcgatcagca
     2641 ccgtcgtgcg cccctgccgt aaccggcgca gggcctgctg cacccgcgcc tcggtcacca
     2701 cgtcgaggtg gctcgtcgcc tcgtcgagca cgagcagggc cgggtcggcg atccaggccc
     2761 gggcgaacgc gatcagctgg cgctcgccgg ccgacagcag cgcgccacgc tcgcccacct
     2821 cggtgtcgaa cccggccggg agcccctcga tcaccgtgtc ggctcccacc gcgcgggcgg
     2881 ccgcctcgac ctcggccctg gtcgcgtccg ggcggccgaa gcggatgttg tcggccaccg
     2941 tcccggagaa cagaaagccc tcctgcggga cgtagcccat ggccgtgcgc aggtccgcga
     3001 ggcggaccgc gcgcaggtcg accccgtcca gggtcacccg cccggcctgg gggtcgtaga
     3061 accgggcgat cagcttcgcc acggtcgact tccccgcgcc ggtcggcccc accagggcga
     3121 cggtggtgcc ggcgggcacg tgcaggtcga cgccgtgcag cacctcgccc ggcggcggca
     3181 ccgcagcgcg gcccacggcc ggccccccgc cgtccgcaac cggccccctg acgtccgcgc
     3241 cgggcgccgc gtaggagaag tgcacgccct cgaggcgaac ctcgccgtcg ggggcgggca
     3301 gggggaccgg ctcggcggcc tcgcggacgg tcggctcctc cgcgagcacc gccccgatcc
     3361 gctcggctcc cgcggtggcc gcctggaagg tgtcgtacag ctccgagagc tgctgcaccg
     3421 ggtcgaacag gctgcgcagg tagagaagga acgcggcgag cgtcccgatc tgcatgcggc
     3481 cgccgagcac ctgggcgccg ccgacgccga ggaccaccac ggtggcggcg gtgccgacga
     3541 actcgatgcc ggggaagaag aacgacgcga gcgcgatcgt ccaccggtgc gcccggcgct
     3601 ccgcgtcgtt ggcgccggcc agccggtcca gggtggcgcc ctcccgccgg aacgcctgga
     3661 tcgcccgcac cccggcgagc gcctcctgcg cgtgcacggt gaccacggag gcggtctccc
     3721 tgacctggcg gtaggcagtg gacgaccagc gccggaacca ggccgcggcc aggccgacga
     3781 gcggcgcgac ggccagggtg gcgagcgcga gccgccagtc gagcgcgacc aggatggcgg
     3841 cgatcccgac cagggtcacg aggctggtca cgaggttgac gagcccgtcg gtgacgaggt
     3901 cgctcatcgc ctccacgtcg ttcgtcatcc tcgccaccag gcgaccggtg cgctcgcgct
     3961 cgtagaagtc cagggagagg gtctgcaggt gcccgaacag gcggcggcgc accgcgaaca
     4021 gcacccgctg cccgaccgcc gccacggtca gggtctccac ccgcccgccg acgagctgga
     4081 ccagggcgat cgccacgtag gcggcggcca cccggtcgac gacctcgagc cggcgcggga
     4141 ccacgcccct gtcgatcgcc accttcacga ggtacggcat ggagagcccg gcagcggtct
     4201 ggatcagcac cgccgccgag gcgaccgcca ccagcccgcg gtggggccgg gccagcggcc
     4261 acagccacgc gcgggcccgg cgtcccgagc ccctgccggg gcgcgcccgc cgttctccag
     4321 acgcgggcct agacacgggc cgcccgcccc tcggtcgcgg cgccctcggc gaccaggcgg
     4381 tcgacgcccg cgttcgagtg cgcgagcacc gcccggtagc ggggctcgcg cccggccagc
     4441 tggtcgtggc gtcccaccgc caccacccgg ccccggtcga gcaggacgat gcggtcggca
     4501 agccgcaggc tggcgcgccg gttggccacg agcagcacgg tcg
//
LOCUS       DASPYY010000485         1662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2329015, whole genome shotgun sequence.
ACCESSION   DASPYY010000485 DASPYY010000000
VERSION     DASPYY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329015"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VEP73_07680"
     CDS             <1..630
                     /locus_tag="VEP73_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26341.1"
                     /translation="ASREDLKAALRAGAWLPIAAGPPVSFRGGRALDGGVLLSHPFRL
                     AVADGCTHVLSLSTLPRGRSRRSVAGGRVIVRWLDRLQPGLGAGYVRALRDDTADRRF
                     LARARTPDGAGGSPPFVLDVAPPPGTPGVKRYELRPDLLLDGARGGYQALVLAVEGRT
                     VAVTPRLTIPDPGLAPAARPSGVAPPRPDPAELVELGDLEGHALFGDDS"
     gene            773..1198
                     /locus_tag="VEP73_07685"
     CDS             773..1198
                     /locus_tag="VEP73_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYT26342.1"
                     /translation="MAQTEQPGAASGPAEVGPVVAGPALRAYQERLQAELDELTVQAE
                     ELEQAANANTAEGSGEVGFDEEFSDAGSYTFERERDLSLSNNARDLIDKIGRALARID
                     RGTFGRCEACGNPIEPDRLDALPYATLCLEDARRRVRLR"
     gene            1198..>1662
                     /gene="lspA"
                     /locus_tag="VEP73_07690"
     CDS             1198..>1662
                     /gene="lspA"
                     /locus_tag="VEP73_07690"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYT26343.1"
                     /translation="MGRTSGRLALVYGTAGLIVTVDQLTKLLVVRTLAGRPPLRLVGS
                     FVDLRYTTNSGGAFSLLPGAPLFFALTALAVTGAIVWNARRAQGRPVLLALGLLLGGA
                     IGNLLDRVFRGEVPLRGEVVDFVKVGPWPVFNVADSCIVIGGLLLALLLGRGE"
BASE COUNT          172 a          639 c          626 g          225 t
ORIGIN      
        1 gcctccaggg aggacctgaa ggccgcgctg cgggctgggg cgtggctgcc gatcgctgcc
       61 ggcccgccgg tctcctttcg cggcggccgg gcactggacg gcggggtgct gctgtcgcat
      121 cccttccggc tcgcggtcgc cgacggctgc acccacgtcc tgtcgctgag caccctgccg
      181 cggggacggt cgcggcggtc ggtcgccggc ggcagggtga tcgtcaggtg gctggaccgc
      241 ctccagcccg ggctcggcgc cggctatgtg cgcgccctga gggacgacac cgcggaccgg
      301 cgcttcctcg cgcgggcgcg cacgccggac ggcgcgggcg gctcgcctcc gttcgtgctg
      361 gacgtggccc cgccgccagg cacgcccggg gtgaagcgct acgagctgcg ccccgacctg
      421 ctccttgacg gtgcccgagg cgggtaccag gccctggtcc tggcggtcga ggggcgcacg
      481 gtcgccgtca cgccgcggct cacgatcccc gaccccggcc tggccccggc cgcccggccc
      541 tccggcgtcg cgccgccacg ccccgacccc gcggagctcg tcgagctggg cgacctggag
      601 ggccacgccc tgttcggcga cgactcgtga cccccggggt ctcgcttgcg cagcttgctc
      661 cctccccggt cgcggcccgc cggtacaatg cgctgtcacc ccggccggag gcggcaccgg
      721 gctgcctgga ggcggcccgc tcgccgccgg gccgggaacg ggaggaggag ccatggcaca
      781 gaccgagcag cccggtgccg cctccggccc ggccgaggtc ggcccggtcg tcgccgggcc
      841 cgccctgcgg gcctaccagg agcggctgca ggccgagctc gacgagctca ccgtgcaggc
      901 cgaggagctc gagcaggcgg ccaacgccaa caccgccgag ggctccggcg aggtcggctt
      961 cgacgaggag ttctccgacg ccggctccta caccttcgag cgggagcgcg acctgtcgct
     1021 gtcgaacaac gcgagagacc tgatcgacaa gatcgggcgg gcgctggccc ggatcgaccg
     1081 cgggaccttc ggccgctgcg aggcgtgcgg caacccgatc gagcccgacc gcctcgacgc
     1141 gctgccgtac gcgaccctct gcctcgagga cgcgcggcgc cgggtgcggc tgcgctgatg
     1201 gggcggacca gcgggcggct cgctctcgtc tacggcaccg ccgggctgat cgtcaccgtc
     1261 gaccagctca ccaagctcct cgtcgtccgg accctggccg gccggccgcc gctgcggctc
     1321 gtgggcagct tcgtcgacct ccgctacacg acgaactcgg ggggcgcctt ctcgctgctg
     1381 cccggcgcgc cgctgttctt cgcgctgacg gcgctcgcgg tcacgggcgc gatcgtgtgg
     1441 aacgcgcggc gggcgcaggg ccgtcccgtg ctgctcgcgc tcgggctgct gctgggcggc
     1501 gcgatcggca acctgctcga ccgggtgttc cgcggcgagg tgcccctgcg cggcgaggtg
     1561 gtcgacttcg tcaaggtcgg gccctggccg gtgttcaacg tggccgactc ctgcatcgtg
     1621 atcggcggtc tgctgctcgc gctcctgctc ggccgcggcg ag
//
LOCUS       DASPYY010000486         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_362680, whole genome shotgun sequence.
ACCESSION   DASPYY010000486 DASPYY010000000
VERSION     DASPYY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362680"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1057)
                     /gene="pth"
                     /locus_tag="VEP73_07695"
     CDS             complement(422..1057)
                     /gene="pth"
                     /locus_tag="VEP73_07695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYT26344.1"
                     /translation="MVSERASRRPGEPSPGARTERWLVVGLGNPGQQYAGNRHNAGSM
                     VADLLAARIGAGFRRDRSRTQAATGRLAGVPVIVAKPTSFMNLSGPPLVALTSFYKIP
                     PDRLVVMHDELDLPFGTIRLKLGGGDGGHNGLRSVSRALAVREYYRVRIGIGRPPGRQ
                     DPADYVLRDFGTAERKELPLVLERAADAVEALVRLGLAAAQNEFHPDPAAS"
     gene            complement(1177..1860)
                     /locus_tag="VEP73_07700"
     CDS             complement(1177..1860)
                     /locus_tag="VEP73_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017613701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HYT26345.1"
                     /translation="MPEVHIAATPRTEFGKGPARRERRAGRVPAVLYGHGAEPRHVSL
                     PGHDVLLALRTANVLIRVDGLAGGSQLALPKAVQRDPIKGTVEHVDLIVVRRGEKVTV
                     DIPVTVTGEIAPDGLLDQQLVQISVEAEATAIPPGVEVNVEGMVVGASVHAGDLQLPP
                     GVTLADESDMLVLHVIPAPTAAQLEAELGVVAEAEEAAEVPEAVGEAAGAEGAAQASP
                     AESSESAGE"
     gene            complement(1948..>2335)
                     /gene="prs"
                     /locus_tag="VEP73_07705"
     CDS             complement(1948..>2335)
                     /gene="prs"
                     /locus_tag="VEP73_07705"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HYT26346.1"
                     /translation="IIHKRRDPDVANKVKVLEVVGQVSGRTCILVDDMIDTGATIANA
                     AETLFEQGATQVIVAATHGVLSGPAVDLLKNSAISEVVVTNTLPIQADKQFDKLTVLS
                     IAPLIARAITEVYSEGSVTSLFDGQS"
BASE COUNT          320 a          841 c          854 g          320 t
ORIGIN      
        1 gctacaggta acaagtcgcc aacattcgct gccgtctcat gactcccacc cgtgcatcag
       61 cctgaacgag cgcgcgcggg acacgccgcc cgccgcctgc ccggccgcct gcccggcagc
      121 gcggggcacc gcggcacccg agcccccggc acccccggcg actccagcgc ccagaatgcg
      181 aagttccccg tcgcagaaat acaacggcta tgtgacatag ccgttgttcg cggagtgccc
      241 agggcgcgtg gcgggccccc ggggggcggc gggggcgctg ggtgcgcggc ggggcccagg
      301 gcgggcgccg cggacgtcgc cgcggacgtc ggcgcccgat gcggccgggc gggcccgggg
      361 gcggccgggc gggccctggg gcggccgtgc gggaccggag gcgcccgggt gggacaagac
      421 gctagctggc ggctggatcg gggtggaact cgttctgcgc ggcggcgagg ccgagccgca
      481 ccagcgcctc gaccgcgtcg gcggcgcgct cgagcacgag cggcagctcc ttgcgctcgg
      541 ccgtgccgaa gtcacgcagc acgtagtcgg ccggatcctg cctgccaggc gggcggccaa
      601 tgccgatcct gacccggtag tactccctga cggccagcgc cctgctcacc gagcgcaggc
      661 cgttgtgccc gccatcgccg ccgccgagct tgagcctgat cgtcccgaac ggcaggtcga
      721 gctcgtcgtg catgacgacg agccggtcgg gcgggatctt gtagaagctg gtcagggcga
      781 ccagcggcgg cccgctcagg ttcatgaacg acgtcggctt ggcgacgatc accggtaccc
      841 cggccagccg cccggtcgcg gcctgggtcc gcgacctgtc ccggcggaag ccggcgccga
      901 ttctggccgc gagcaggtca gcgaccatgg acccggcgtt gtgccggttg cctgcgtact
      961 gctggcccgg gttgccgagg ccgaccacca gccaccgttc cgtgcgggcg ccgggggacg
     1021 gttccccggg ccgccgggat gctcgctcgc tgaccatctc gtcacgatcg gccgccgcgg
     1081 accggcccag ccgaccgcac ctgcccggcc ggcgcgaccg gcccggccgg cccggccaga
     1141 gcggccagcc catcagccag cccgcccgct gctcgcttac tcgccggccg actcggagga
     1201 ctcggcgggg gatgcctggg cggcgccctc ggcgccggca gcctcgccga ccgcctcggg
     1261 aacctcggcg gcctcttccg cctcggcgac gacgccgagc tcggcctcga gctgcgccgc
     1321 ggtcggagcc gggatgacgt ggagcacgag catgtcggac tcgtcagcga gcgtgacgcc
     1381 cggcggcagc tggaggtcgc cggcgtgcac cgacgcgccg accaccatgc cctcgacgtt
     1441 gacctcgacg cccggcggga tcgcggtcgc ctcggcctcg acggaaatct gaaccagctg
     1501 ctggtcaagc aggccgtcgg gggcgatctc gccggtgacg gtgaccggaa tgtcgacggt
     1561 gaccttctcg ccgcggcgta ccacgatgag gtcgacgtgc tcgacggtgc ccttgatcgg
     1621 gtcgcgctgc acggccttcg gcagcgcgag ctggcttccg ccggccaggc catcgacccg
     1681 gatcagcacg ttcgctgtgc gcagcgccag cagtacgtcg tggccgggca ggctgacgtg
     1741 acgcggctcg gcgccgtggc cgtagaggac agcgggaact cgcccggcac ggcgctcgcg
     1801 ccgggcggga cccttgccga actcggtccg cggcgttgcg gcgatatgaa cctcgggcac
     1861 agtgccactc ctcagggaaa acggatatga gcccagtcac gggcagaaga attcgcagtg
     1921 attctagccg acaatccgcc cggggggtca gctctggccg tcgaagaggc tcgtcaccga
     1981 gccctccgag tagacctcgg tgatggccct ggcgatcagc ggggcgatgg acagcaccgt
     2041 gagcttgtcg aactgcttgt cggcctggat cggcagcgta ttggtgacga ccacctcgct
     2101 gatggccgag ttcttcagca ggtcgacggc cggcccggac agcacgccgt gcgtggcagc
     2161 gacgatgacc tgggtcgcgc cctgctcgaa cagcgtctcg gctgcgttgg cgatcgtcgc
     2221 gccggtgtcg atcatgtcgt ccacgaggat gcacgtccgc ccggagacct ggccaacgac
     2281 ctcgagcacc ttcaccttgt tggcgacgtc gggatcgcgg cgcttgtgga tgatc
//
LOCUS       DASPYY010000487         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1065592, whole genome shotgun sequence.
ACCESSION   DASPYY010000487 DASPYY010000000
VERSION     DASPYY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065592"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VEP73_07710"
     CDS             <1..159
                     /locus_tag="VEP73_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYT26347.1"
                     /translation="LAILACPNCRGEVDYKEAEQVIECRQCHYRYPVRDDIPVMLVDE
                     AEKPEPAN"
     gene            156..1244
                     /locus_tag="VEP73_07715"
     CDS             156..1244
                     /locus_tag="VEP73_07715"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HYT26348.1"
                     /translation="MTSADLDDPGALAAADPSGILAAVAGAGGQARAARAAAPAAALA
                     GPLPEVVVVAGMGGSGVAGDVLAALAFAESAVPVVTVKGDRLPAFVGPGTLLVAVSYS
                     GDTDETLSAVLQGQEQGARLVAVTSGGRLGELAERRGAPLVLVPDGYQPRAAFWSLVV
                     PVLLAAEGSGVLRPLGGQVTAAADALDAEAAELGPTVPAARNPAKQAALRLAGRLPVV
                     WGTGQLGAVAANRFRTQCNENAKIPVVSAAVPEADHNDVMGLESGIGPDRELVLLRDP
                     AGEHERDARRVAAACEALGAGDPIVRRSDTRTSPAPGGRAPLARLARLVAFADFTSTY
                     LGIARGVDPASIATIARIKAALAAEARR"
     gene            1241..2053
                     /locus_tag="VEP73_07720"
     CDS             1241..2053
                     /locus_tag="VEP73_07720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006636490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HYT26349.1"
                     /translation="MSAPPVPRAVTTAAVSLASRLREKPAVALVLGSGLGDLAEEVED
                     AVRVPAAEVPGMPVPRVPGHAGAVVGGRLAGVPVLVLAGRVHTYEGYSASEVAFPTRV
                     AAELGCRALVATNAAGGLNLDFRPADLMVLTDHVNFTFDNPLRGGPWFVDLTGAYDAD
                     LRAEAVRHAESAGIPVRQGVYLATAGPSYETPAEVAMFRAWGADAVGMSTVPEVIAAR
                     AHGMRVAAVSAITNVHRPGGTPVSHAEVLEVAERIRPRFRELVRSLLPAAAA"
     gene            2429..2569
                     /locus_tag="VEP73_07725"
     CDS             2429..2569
                     /locus_tag="VEP73_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26350.1"
                     /translation="MKWDDPETSVDWRRQVAMLVLERVEVASARRVGRDLRARVDPEW
                     KA"
     gene            complement(2609..2845)
                     /locus_tag="VEP73_07730"
     CDS             complement(2609..2845)
                     /locus_tag="VEP73_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26351.1"
                     /translation="MSHFLVVYDRSTGELRELTEFSEDETDRASRARLERELVERTHP
                     EVEVVVLTSPSRAALERTHARYFKSVHELTGTGL"
     gene            complement(2842..3513)
                     /locus_tag="VEP73_07735"
     CDS             complement(2842..3513)
                     /locus_tag="VEP73_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26352.1"
                     /translation="MTELSNRQIDRLGDRLRISDPTAKDLELLGQVRGLYAGALAEVT
                     RVLVDLGYEPGARLKTVGTLVDKLRRIPEPLSRIQDVAGARVVVEMNREEQDRLVERI
                     TARFDHYRVKDRRVDPSFGYRAVRYGFPPDEPDLLIGQQVTRQRVINVVMELAETIDD
                     AEAGRLEVIALEREIASTFGGTPTAELESALARVHLHANEAERVVRSALELLHSGSTM
                     DTLTR"
     gene            complement(3780..4241)
                     /locus_tag="VEP73_07740"
     CDS             complement(3780..4241)
                     /locus_tag="VEP73_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT26353.1"
                     /translation="MAMTATVGLTREAIDRIIQDHFRQEAAGDVAGTLSTFTDDVVHD
                     VVGDAGGELHGPAAVGQRYGHLFSNVKGEHADVKHRLYGHNFVVDDMIWTARVVGEFM
                     GIPGHGRRISIRVLHVFEFRDGLISRENVWIDAGAAIAQLTSEAGAEPTDG"
     gene            4305..4961
                     /locus_tag="VEP73_07745"
     CDS             4305..4961
                     /locus_tag="VEP73_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26354.1"
                     /translation="MPRVNARRKYSSPLRERQAKATRRAVLAAARQLFIAQGYGATTV
                     EQIAQHAGVSKPTVFSAVGNKQMVLRAVRDVAIAGDDEPVPVAKRPATDRIRAEPDQR
                     RAVALLAQHLTGVASRYAEINEVLHAAADSGEDDLRELWKLEEDQRLTGARLWVDVLT
                     SKGPLRPGLDTGTAVDVLWLLMSPDNYYRLVHRRRWTNQQYQQWLAASITQLLSAEHP
                     "
     gene            complement(5028..5243)
                     /locus_tag="VEP73_07750"
     CDS             complement(5028..5243)
                     /locus_tag="VEP73_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYT26355.1"
                     /translation="MSERQELRLTAVVTPDEDWFMARCLEVPVTSQGPTREAALANLR
                     EALELYLEDEPVPAPADVERIDVRLSA"
     gene            5351..>6071
                     /locus_tag="VEP73_07755"
     CDS             5351..>6071
                     /locus_tag="VEP73_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT26356.1"
                     /translation="MTTLSPNRPGASPGPAPGPGHGVPRPTMRDVAEAAAVSLKTVSR
                     VVNDEAGVRPDTAARVREAIATLGFRRNDMARVLRQGQASRTLGLVIEDVGNPFYSVV
                     TRAVEEVARERGYLVITGSSDEDPARERDLIRSLVERRVDGLLVVPAGGDHRFLVPEL
                     RLGTAVVFMDRPPGNIDADVILLDNVGGARRATEHLLDQGHRRVAMVGDAPAIFTSAE
                     RLAGYRQALAARGVGVEERLVR"
BASE COUNT          811 a         2249 c         2159 g          852 t
ORIGIN      
        1 ctcgccatcc tcgcctgccc caactgccgg ggcgaggtcg actacaagga ggccgagcag
       61 gtcatcgagt gccgccagtg ccactaccgc tacccggtcc gcgacgacat ccccgtcatg
      121 ctggtggacg aggccgagaa gccagagcca gccaattgac ctcggccgac ctggacgacc
      181 ccggcgcgct ggccgcggcc gacccctcgg ggatactggc ggcggtggcc ggcgcgggcg
      241 gccaggcgag ggccgcccgc gcggctgcgc cggcggcggc gctggccggc ccgctgcccg
      301 aggtcgtcgt ggtcgccggg atggggggct ccggggttgc cggggacgtc ctcgccgcgc
      361 tcgccttcgc cgagtcggcg gtcccggtcg tgaccgtgaa gggggaccgg ctgcccgcct
      421 tcgtcgggcc cgggaccctg ctggtggcgg tgtcgtactc cggcgacacc gacgagacgc
      481 tctccgccgt cctgcagggc caggagcagg gcgcccgcct ggtcgcggtc acctccgggg
      541 gccgcctggg cgagctcgcc gagcggcgcg gcgccccgct cgtgctcgtc ccggacgggt
      601 accagccgcg ggccgcgttc tggtccctgg tcgtccccgt gctgctcgcc gccgagggct
      661 ccggggtgct ccgtccgctc ggggggcagg tgacggcggc ggccgacgcg ctcgacgccg
      721 aggcggccga gctcgggccg accgtgccgg ccgcccggaa cccggccaag caggccgccc
      781 tccgcctggc tggccggctc cccgtcgtgt gggggaccgg ccagctcggc gcggtcgccg
      841 cgaaccggtt ccggacccag tgcaacgaga acgccaagat cccggtggtc tccgccgccg
      901 tgccagaggc cgaccacaac gacgtgatgg gcctggagag cggcatcggc cccgaccgcg
      961 agctggtgct gctgcgcgac ccggccggcg agcacgagcg cgacgcgcgg cgggtcgccg
     1021 ccgcgtgcga ggcgctcggg gccggcgacc cgatcgtgcg ccgctccgac acccggacct
     1081 cgcccgctcc aggcgggcgt gccccgctgg cccgcctcgc gcgcctggtg gcgttcgccg
     1141 acttcacctc cacctacctg ggcatcgccc gcggcgtcga ccccgcgtcc atcgccacca
     1201 tcgcccggat caaggcggcc ctggccgccg aggcccgtcg gtgagcgcgc cgccggtgcc
     1261 ccgggcggtc accaccgcgg ccgtctcgct ggcgtcgcgt ctgcgggaga agccggcggt
     1321 cgcgctggtg ctcgggtccg ggctcgggga cctggccgag gaggtcgagg acgcggtcag
     1381 ggtcccggcc gccgaggtgc ccggcatgcc ggtgccgagg gtgcccggcc acgccggcgc
     1441 cgtcgtcggc ggccggctcg ccggggtgcc cgtgctcgtc ctcgccggcc gggtccacac
     1501 ctacgagggc tactccgcct ccgaggtcgc gttcccgacc agggtggcgg ccgagctggg
     1561 ctgccgggcg ctggtggcca cgaacgcggc gggcggcctg aacctcgact tccggccggc
     1621 cgacctgatg gtcctcaccg accacgtcaa cttcacgttc gacaacccgc tgcggggcgg
     1681 gccgtggttc gtcgacctga ccggcgccta cgacgcggac ctgcgggccg aggcggtgcg
     1741 ccacgcggag tcggccggca tccccgtccg ccagggcgtg tacctggcca ccgccggccc
     1801 ttcctacgag accccggccg aggtcgccat gttccgggcc tggggcgccg acgcggtcgg
     1861 catgtccacc gtgcccgagg tgatcgccgc ccgcgcccac ggcatgcggg tcgcggccgt
     1921 ctcggcgatc accaacgtgc accggccggg cggcacgcct gtcagccacg ccgaggtcct
     1981 cgaggtcgcc gagcggatcc gcccccgctt ccgggagctc gtgcgctccc tcctcccggc
     2041 cgcagcggcc tgaccggcgt gccgcccgac ccccggacag ggtccggcgt gacgaaccga
     2101 gcattcgagc gctcctgccc caggtatggg agacggccga gtggctcttc atgcacatga
     2161 cgcgtgctac ctggatcgag ctgttccgcc cggctcgtca ccgacccggt gacggccccg
     2221 ctcgtcgccc aggccgcggc gcgcgtgctg aagggcgaga cggtcggcac gatcctccgc
     2281 gagtggactg gccggcgatc atcgaccgcg gccagcatga gcggctgctc gcggtcttcg
     2341 ccgacaaggg cgccgccgcc agggccgcgg ccggtcgccc gccgcggcgg tcctcgccga
     2401 cctgccccgc acgaaggcgg agctgcgcgt gaagtgggat gacccggaga cctcggtcga
     2461 ctggcgccgg caggtcgcca tgctggtcct cgagcgcgtc gaggtggcga gcgcccggcg
     2521 ggtcggtcgc gacctgcgcg cccgggtcga cccagagtgg aaggcgtgac tcggcgccga
     2581 cgcgcgtggc cgagggcggc cgcgagacct agagcccggt cccggtgagc tcgtgcacgc
     2641 tcttgaagta gcgggcatgg gtccgctcga gcgcagcccg ggacggggac gtgagcacga
     2701 cgacctcgac ctcgggatgg gtacgctcga cgagctcccg ctcgaggcgc gcccgggagg
     2761 cacgatcggt ctcgtcctcg ctgaactcgg tgagctcgcg cagctcaccg gttgagcggt
     2821 cgtagacgac gaggaagtgg ctcatctcgt cagtgtatcc atggtgctcc cactgtgaag
     2881 tagctccagg gccgacctga ccacccgttc cgcctcattc gcgtggaggt gcacccgtgc
     2941 aagcgcgctc tcgagctcgg cggtaggtgt tcccccgaaa gtgctggcaa tctcgcgttc
     3001 caacgcgatg acctcgagcc ggcccgcttc ggcatcgtcg atcgtctcgg cgagctccat
     3061 cacgacattg atgactcgct gacgcgtgac ctgttgtccg atgagcaggt caggctcgtc
     3121 aggcgggaag ccgtagcgga cggcccggta gccgaagctc gggtcgacgc gccggtcctt
     3181 gacccggtag tggtcaaatc gggccgtgat ccgctcgacg aggcggtcct gttcctcccg
     3241 gttcatctcg accacgacac gggcgccggc aacgtcctgg atgcgggaca acggctcggg
     3301 gatacggcgc agcttgtcga ccagcgtgcc gaccgtcttc agccgggcgc ccggctcgta
     3361 gcccaggtcg accaggacgc gcgtgacctc ggcgagcgcc cccgcgtaca agcctcgcac
     3421 ctgcccgagc agctcgagat ccttggcagt cgggtccgag atgcggagtc gatcgccgag
     3481 ccggtcaatc tgcctgttgg agagctcggt caccgccggg gaaagccata gagtgacatc
     3541 atgggcgggg tccgctccga cccggagcgg cacgtcaaga tcaatacgtc aagatcaagt
     3601 ggaaagcgtg agcggtggac tccttcacga gtcgcgctcc caccgcacca cgcagcgtct
     3661 gcccgcatcc gccgtgatcc ggcgtgctgg acgctacctc cgcgctgggt gcgccgcgcc
     3721 gcgaccggta tggtcgccgc tgagcaggcc cagcgagacg agtccgctta ccagaggggc
     3781 tatccgtcgg tgggttcggc tcccgcctcg gacgtcagct gcgcgatggc cgcgccggcg
     3841 tcgatccaga cgttctcccg cgagatgagc ccgtcgcgga actcgaacac gtgcagcacg
     3901 cggatgctga tgcggcgccc atggcctggg atgcccatga actccccaac cacccgtgcc
     3961 gtccagatca tgtcgtcgac cacgaagttg tggccgtaga gacggtgctt gacgtcggcg
     4021 tgctcaccct tgacgttgct gaacaggtgc ccgtagcgct ggcccaccgc ggctggcccg
     4081 tgcaactcgc cgcccgcgtc accgacgacg tcgtggacga cgtcgtcggt gaacgtgctg
     4141 agcgtgccag cgacgtcgcc ggcggcttcc tgtcgaaagt ggtcctggat gatccggtcg
     4201 atcgcctccc gcgtgaggcc gacggttgcc gtcatcgcca tcctctcccc tgtcatttgg
     4261 ctttaccgat catctagcga acactcgcat ggtaaactga agaggtgccc cgagtcaacg
     4321 cccgccggaa gtactcgtcg ccacttcgcg agcgccaggc caaggcgacc cgccgtgctg
     4381 tgctggcggc ggcacggcag ttgttcatcg cccagggcta tggggcaacc accgtcgaac
     4441 agatcgccca gcacgccggg gtgagcaagc cgacggtgtt cagcgcggtg ggcaacaagc
     4501 agatggtgct gcgcgcggtg cgcgacgtgg cgatcgcagg cgacgacgag cccgtcccgg
     4561 tcgccaagcg cccggcgacc gaccggattc gtgccgaacc ggaccagcgc cgcgctgtcg
     4621 cgctcctggc ccagcacctc accggcgtgg ccagccgcta tgccgagatc aacgaggtct
     4681 tgcacgcagc cgccgacagt ggcgaggacg acctccgcga actctggaag ctcgaggagg
     4741 accagcgcct cacgggcgcg cgcctgtggg tcgacgtgct caccagcaaa gggccgctgc
     4801 gacccgggct cgacaccggc accgcagtcg acgtgctgtg gttgctgatg tcacccgaca
     4861 actactaccg gctcgtgcac cggcggcgct ggacgaacca gcagtaccag cagtggctgg
     4921 ccgcctcgat cacgcagctg ttgtctgcgg agcatccctg agccaccatg ccggcgcggg
     4981 tgagggcacg cttcaggcgc gcgccccgga gacgtcaggc agccccgtca cgccgacagg
     5041 cgcacatcga tgcgctcgac atccgcggga gccggcaccg gctcgtcctc aaggtacagc
     5101 tcgagcgctt cacgcagatt cgcaagcgcc gcctcgcgcg tcggcccttg gctcgtgacc
     5161 ggcacctcca ggcacctggc catgaaccag tcctcgtcag gcgtgacgac cgccgtcagg
     5221 cggagctcct gccgctcgct catcggtccc ccgttcccgg tcagggcacc ttgtcgttga
     5281 ccgccgggca acgcctgact gtcgcagtgt acttgacacc cggccccgcc ggtggcttca
     5341 ttgcacgacg atgacaacgt tgtcgcccaa ccggccgggc gcctcgcccg gccctgctcc
     5401 cggccctggt cacggcgtcc cgcgccccac catgcgggac gtcgccgagg cagccgcggt
     5461 cagcctgaag accgtctccc gggtggtgaa cgacgaggcc ggcgtgcggc ccgacaccgc
     5521 ggcgcgcgtg cgggaggcca tcgccacgct cggcttccgg cgcaacgaca tggccagggt
     5581 gctgcgccag ggccaggcgt cgcgcacgct ggggctcgtg atcgaggacg tcggcaaccc
     5641 gttctactcg gtcgtcaccc gggcggtcga agaggtggcg cgcgagcgcg gctacctggt
     5701 gatcaccggc agctccgacg aggacccggc gcgcgagcgc gacctcatcc gttcgctggt
     5761 cgagcggcgc gtcgacgggc tgctggtggt ccccgccggc ggcgaccacc gcttcctcgt
     5821 ccccgagctg cggctcggga ccgcggtggt gttcatggac cgaccgccag gcaacatcga
     5881 cgccgacgtc atcctgctcg acaacgtcgg cggcgcccgg cgggccacgg agcacctgct
     5941 cgaccagggc caccgccgcg tcgccatggt gggtgacgcg ccggccatct tcacctcggc
     6001 cgagcgcctg gccggctacc gccaggccct tgcggcccgg ggcgtcgggg tcgaggagcg
     6061 gctggtccgc g
//
LOCUS       DASPYY010000488         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2190073, whole genome shotgun sequence.
ACCESSION   DASPYY010000488 DASPYY010000000
VERSION     DASPYY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190073"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VEP73_07760"
     CDS             complement(<1..1005)
                     /locus_tag="VEP73_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26357.1"
                     /translation="MATLGLGLLAWAGLALAHAGRYSPPAAFGLATAALAAVALAAWR
                     AAGRPRVLLDPPGLAMAAGVALLAALLFFPGFPYGAGDKDPGGYVSQGILIARTGSYA
                     MDDPVLDKARIPTVALSSPGARLPGQWIEDARVPRRTIPQFYHLWSAALASAYAIGGY
                     TGLVNLNPLCGVLAAMVLALAARRVFGLLAGSVAGVLLAADMLETWQAKYQTSEVFTQ
                     LLVLGALLGVVLALRTGWRPAAGVAGLLLGLSFLARADSLVLVLAAVGAGCVLIATGR
                     FDGRAAWFAGGLAVTLPHGLLQAYRFARLYTLSVHLPSAAEVAVAVVAPLALAVVLRL
                     A"
     gene            1235..1888
                     /locus_tag="VEP73_07765"
     CDS             1235..1888
                     /locus_tag="VEP73_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HYT26358.1"
                     /translation="MFRERIDRQLWREREVSLLASWRGRLRYELRHRIPVSVGSPTVI
                     DDPRRLEFRNDGHLRIGIGSFGLTTSRDVTVIRIRPGATFVVDGQVNLHRGSRIVVDA
                     GQLEIGHQTMVNGLTKVLVSTGVRIGSGCNIAWNVQILDTDFHSVYVDGEPLPKTAPI
                     DIGDRVWIGTNALVLKGVTIGDGAVVAAGSVVTRDVPPKTLVAGVPAKPVRQVEHWQ"
     gene            1910..2806
                     /locus_tag="VEP73_07770"
     CDS             1910..2806
                     /locus_tag="VEP73_07770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT26359.1"
                     /translation="MTPPGGRAGAPAGTRERFRPYVPLLRGYGPVLQLACGRGELLDL
                     LADAGEAATGVEPDEGPAREARRRGRDVVHADVLGYLEADPAPGPFRAVVCAGLVERL
                     EPPQALRVLAGARRVLAPGGRLLAATANPASYPVVSRRFWNDPRGTRLYDVRLLEYLC
                     TRAGFVVERSAGNPADHPAPPAEVLGDAEPVVHPGLADAIGGSGARVQAGMEHRHREL
                     PAAEAHDPGWAFDLLHAVKTLSDRLTETQEAARELDRVHRELVRGMYRPAEVYVVARA
                     PVGASRASGNRVPQAGLDEEDA"
     gene            complement(2759..3631)
                     /gene="rfbD"
                     /locus_tag="VEP73_07775"
     CDS             complement(2759..3631)
                     /gene="rfbD"
                     /locus_tag="VEP73_07775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HYT26360.1"
                     /translation="MRVLVTGAGGQVGGDLVAAFGDAKEVVAATRADLDVGDRHAVAA
                     ALAELEPDLVLNAAAYTDVDGCETEVDRAFRDNCLGPRHLALACREVDAELVHLSTDY
                     VFDGTAGADRPDGYGEYDTPAPASVYGRSKLAGEQAVRDVWDRHYVVRTAWVYGATGR
                     NFVRTVLRLAAERPVLRVVDDQVGSPTWSRDLAQGVRALVRTGQHGTYHLTGGGSCSW
                     YDLARAVLAGTGQDPGRVEPTTSAEFPRPAPRPAYSVLDNRLARLVGVPPLRPWDEAL
                     RVFLIETGLGNPIA"
     gene            complement(3637..>4132)
                     /locus_tag="VEP73_07780"
     CDS             complement(3637..>4132)
                     /locus_tag="VEP73_07780"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYT26361.1"
                     /translation="FPEKVVPLFVTNLIDGHRVPLYGSGGNIRDWLYVLDHCTAVDLV
                     LRRGETGRVYNVAAGSEVTNLELTRRLLAAFGAGEDMVEYVTDRPGHDWRYSLDATRL
                     RELGWAPAHDFDEGLAETIAWYRDNEDWWRPLKPAGATRTLDHGPPAAPHGQGPDRPA
                     RPGV"
BASE COUNT          547 a         1502 c         1611 g          472 t
ORIGIN      
        1 ggcgagccgc agcacgaccg cgagcgcgag cggcgccacg acggccacgg cgacctctgc
       61 cgcgctcggc aggtggaccg agagggtgta gagcctggcg aaccggtacg cctgcagcag
      121 cccgtggggc agggtcaccg cgaggccgcc cgcgaaccag gccgcccggc cgtcgaaccg
      181 gccggtcgcg atcagcacgc agccggcgcc caccgcggcg aggacgagca ccaggctgtc
      241 ggctctggcg aggaagctca gcccgagcag caggccggcc acgccggcgg ccgggcgcca
      301 gccggtccgc agcgccagca cgaccccgag cagcgcgccg aggaccagga gctgggtgaa
      361 gacctcgctc gtctggtact tggcctgcca ggtctcgagc atgtcggcgg cgagcagcac
      421 gccggccacc gagccggcga gcaggccgaa cacccgccgc gcggccaggg cgaggaccat
      481 cgcggccagc accccgcaca gggggttcag gttgacgagg ccggtgtacc cgccgatcgc
      541 gtacgcgctg gccagcgcgg ccgaccacag gtggtagaac tgcgggatcg tgcggcgggg
      601 cacgcgcgcg tcctcgatcc actggccggg caggcgggcg ccaggggagg acagggccac
      661 ggtggggatg cgcgccttgt cgagcaccgg gtcgtccatc gcgtaggagc ccgtgcgggc
      721 gatgaggatg ccctgggaga cgtagccgcc gggatccttg tcccccgcgc cgtaggggaa
      781 ccccgggaag aacagcagcg cggcgaggag cgcgacgccg gccgccatgg cgagaccggg
      841 cgggtcgagc aggacccggg ggcggccggc ggcacgccag gcggcgagcg cgacagcggc
      901 aagggccgcc gtcgcgaggc cgaacgcggc gggcggcgag taccgtcccg cgtgggcgag
      961 cgccaggccg gcccaggcca ggaggccgag gccgagggtg gccatgccta ccaggacctc
     1021 cgcggcaacg aggacgggac cggcggccgg ccgtccgccc gggacgtcgc cggcggcacc
     1081 gggtgtggtc tcgacctgtg aaaccgccat gaggcgggag tatacgcaag tcctctcgcg
     1141 ggccagccgc gttgtctcga cccgtggaga agtgagatgg tcggaagccg tcgacaggag
     1201 gcgggatcgg acgtacaaga aggggtgccc tgcgatgttc cgtgagcgga tcgaccggca
     1261 actgtggcgc gagcgggagg tgtccctgct cgcgagctgg cgaggccgcc tccgctacga
     1321 gctgcgccac cgcatcccgg tgtcggtggg ctcccccacg gtcatcgacg acccgcgccg
     1381 gctcgagttc cgcaacgacg ggcacctgcg catcggcatc ggcagcttcg ggctgacgac
     1441 gagccgggac gtcaccgtga tccggatccg cccgggggcc accttcgtgg tcgacgggca
     1501 ggtgaacctg caccgcggct cccgcatcgt cgtggacgcc ggccagctcg agatcggcca
     1561 ccagacgatg gtcaacgggc tcaccaaggt gctggtcagc acgggcgtcc gcatcgggtc
     1621 gggttgcaac atcgcctgga acgtgcagat cctggacacg gacttccact cggtctacgt
     1681 cgacggggaa ccgctgccga agaccgcgcc gatcgacatc ggcgaccggg tctggatcgg
     1741 gaccaacgcg ctcgtgctca agggggtgac catcggtgac ggtgccgtcg tggcggccgg
     1801 ctcggtcgtc acccgcgacg tgcccccgaa gacgctcgtg gccggagtcc cggccaagcc
     1861 ggtcaggcag gtcgagcact ggcagtgacc ccggccccgg ggaggcgccg tgacgcctcc
     1921 cggcggccgg gccggtgcgc ccgcggggac acgcgagcgg ttccggccct acgtccccct
     1981 gctccgggga tacgggccgg tgctccagct cgcctgcggc cggggcgagc tcctcgacct
     2041 gctcgccgac gccggggagg cggcgacggg cgtcgagccc gacgagggcc cggcgcgcga
     2101 ggccaggcgc cgcggccggg acgtggtgca cgccgacgtg ctcgggtacc tggaggccga
     2161 cccggcgccc gggccgttcc gggccgtggt ctgcgccggc ctggtcgagc ggctcgagcc
     2221 cccgcaggcc ctccgcgtgc tcgccggcgc tcgccgcgtg ctcgccccgg gcggccggct
     2281 cctggcggcc accgccaacc cggcctccta cccggtcgtg agccgccgct tctggaacga
     2341 cccgcgcggc acccggctct acgacgtgcg gctgctcgag tacctctgta cgcgggccgg
     2401 gttcgtcgtg gagcgctcgg ccggcaaccc ggccgaccac ccggcgcccc cggcggaggt
     2461 cctcggcgac gccgagccgg tggtgcaccc ggggctggcc gacgccatcg gcgggtcggg
     2521 ggcccgggtc caggccggca tggagcacag gcaccgcgag ctgcccgccg cggaggccca
     2581 cgaccccggg tgggccttcg acctcctgca cgcggtcaag acgctgtccg accggctcac
     2641 cgagacccag gaggcggcgc gcgagctgga ccgggtgcac cgcgagctgg tccgcggcat
     2701 gtaccggccg gccgaggtgt acgtggtcgc ccgggccccc gtcggggcgt cgcgcgcttc
     2761 aggcaatcgg gttccccagg ccggtctcga tgaggaagac gcgtagggcc tcgtcccagg
     2821 ggcgcagcgg ggggacgccg accaggcgcg ccagccggtt gtcgaggacg gagtacgcgg
     2881 gccggggcgc cggccggggg aactcggcgg aggtcgtcgg ctcgacccgg cccgggtcct
     2941 ggccggtgcc ggccaggacg gccctggcca ggtcatacca ggagcacgac ccgccgcccg
     3001 tgaggtggta ggtgccgtgc tgcccggtgc gcacgagcgc ccgcacgccc tgggccaggt
     3061 cgcgcgacca ggtggggctg cccacctggt cgtcgaccac gcgcaggacg ggtcgctcgg
     3121 cggccaggcg cagcaccgtg cggacgaagt tccggccggt cgccccgtac acccaggcgg
     3181 tgcgcacgac gtagtggcgg tcccagacgt cccggacggc ctgctcgccc gcgagcttcg
     3241 agcggccgta gacgctcgcc ggggcggggg tgtcgtactc accgtagccg tccggccggt
     3301 ccgcgccggc cgtgccgtcg aagacgtagt cggtggagag gtggacgagc tcggcgtcga
     3361 cctcccggca ggcgagggcg aggtgccgcg gcccgaggca gttgtcgcgg aaggcgcggt
     3421 ccacttcggt ctcgcagccg tcgacgtcgg tgtaggcggc cgcgttgagg acgaggtcgg
     3481 gctcgagctc cgcgagagcg gcggcgaccg cgtgccggtc gccgacgtcg aggtcggccc
     3541 gcgtcgcggc gacgacctcc ttggcgtcgc cgaacgcggc cacgaggtcg ccgccgacct
     3601 ggccgccggc cccggtgacg aggacccgca cgccgctcag acccccggcc gcgccggacg
     3661 gtcgggcccc tggccgtgcg gcgcggccgg ggggccatgg tcgagggtcc tcgtcgcccc
     3721 ggccggcttc agcgggcgcc accagtcctc gttgtcccgg taccaggcga tcgtctcggc
     3781 cagcccttcg tcgaagtcgt gcgccggcgc ccagccgagc tcccggagcc tggtcgcgtc
     3841 gagcgagtag cgccagtcgt ggcccggccg gtcggtgacg tactcgacca tgtcctcgcc
     3901 ggccccgaac gcggcgagca ggcgccgggt cagctcgagg ttcgtgacct cgctgcccgc
     3961 cgcgacgttg tagacccggc ccgtctcccc gcggcgcagc acgaggtcga cggcggtgca
     4021 gtggtcgagc acgtacagcc agtcccggat gttcccgccg ctgccgtaca gggggaccct
     4081 gtggccgtcg atcaggttcg tcacgaacag ggggacgacc ttctcgggga ac
//
LOCUS       DASPYY010000489         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_224154, whole genome shotgun sequence.
ACCESSION   DASPYY010000489 DASPYY010000000
VERSION     DASPYY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224154"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VEP73_07785"
     CDS             complement(<1..735)
                     /locus_tag="VEP73_07785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT26362.1"
                     /translation="MVSTARVAVVTGAAQGIGRRVAEVLAGEGYALALTDLREPAEAL
                     GAVRARGVEALGVAGDVAAEADVAALAERVGERFGRMDVLVNNAGISLLAPAEDTAPA
                     QWRRVLEVNLTGPFLLCQQFGQMMLRPGGGSIVNVASIAGLQGIADRAAYNASKHGLV
                     GLTRTLAAEWGGRGIRVNAVCPAWVKTEMDVADQATGAYTDGDILGQVPMGRFASPDD
                     VGAAVAFLADPARSGFINGVALPVDGG"
     gene            complement(817..1290)
                     /locus_tag="VEP73_07790"
     CDS             complement(817..1290)
                     /locus_tag="VEP73_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT26363.1"
                     /translation="MASAASTLAAIERAMVRIRRSQTRRTLGRLATRDLGDAVDLAQL
                     DAVLAVDEGPEQPGQEVTVGLVAERLGVDPSRASRLVAAAIRAGHLRRVASQADGRRI
                     HLETTDAGREVAEAAHRARQALYDRLMRDWSQRDRREFARLLGRFTDALTDAGRT"
     gene            1459..1764
                     /locus_tag="VEP73_07795"
     CDS             1459..1764
                     /locus_tag="VEP73_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26364.1"
                     /translation="MNQRVLLVGRSRRVLSKLTSALTEAGFDAQWTEDPATASQRVQP
                     PSVDLVAFGRGVGSQDRISQQVATGMHSIPLGRQAAGQGPKFAVVRVDATEVSIVKL"
     gene            1839..2360
                     /locus_tag="VEP73_07800"
     CDS             1839..2360
                     /locus_tag="VEP73_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYT26365.1"
                     /translation="MAAGATDPGTEVVDLVDAEDRVVGRAPRRVVRRDKLLHRGVGVL
                     CRNSAGEIYVHRRTDTKDVFPGMYDMLAGGVVSAGETYEATARREIAEELGIVGPEPR
                     LLFKHRYTGADNPSWIAIFEVVWDGPIRHQAEEIAWGAFLTPDELLRKLDEWPFVPDS
                     LEGFRLYRAGKLP"
     gene            2644..>3313
                     /locus_tag="VEP73_07805"
     CDS             2644..>3313
                     /locus_tag="VEP73_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26366.1"
                     /translation="MLGPDCGLGAGMGKQVVLISEDQKLGSILMIALVDPGRLVTVGA
                     ASELPEGGEEAIEAIVLDLPLDSRRRAYAEVRERFAGRVLVPVDTGSETSDWPPDPDR
                     HVLVRPFHLADLIAGVQGPDRPQRGEAEGQRRRSLRRLRSGVPWPVPARGAGGVAAAP
                     PAAEPAPGPAAGRRPRRVAGTATAALAVLLAGIGAGMLLKPDPPRPASEAPGPVVRQL
                     VVTRP"
BASE COUNT          460 a         1196 c         1204 g          453 t
ORIGIN      
        1 gccgccgtcg accggcagcg cgaccccgtt gatgaagccg ctgcgtgccg ggtcggccag
       61 gaacgcgacc gcggcgccga cgtcgtcggg ggaggcgaac cggcccatcg gcacctgccc
      121 gaggatgtca ccgtcggtgt aggcgccggt ggcctggtcg gcgacgtcca tctcggtctt
      181 cacccaggcg gggcacaccg cgttcacccg tatgccccgc ccgccccact cggcggccag
      241 cgtgcgggtc agaccgacca ggccgtgctt gctggcgttg taggcggcgc ggtcggcgat
      301 gccctgcagc ccggcgatcg aggcgacgtt gacgatcgac ccgccgccgg gccgcagcat
      361 catctggccg aactgctggc acagcaggaa cgggccggtc aggttcacct cgagcacccg
      421 ccgccactgt gcgggcgcgg tgtcctcggc cggcgcgagc agcgagatgc cggcgttgtt
      481 gaccagcacg tccatgcggc cgaaccgctc ccccacccgt tcggccaatg ccgctacgtc
      541 ggcctctgcc gcgacgtcac cggcgacccc gagcgcctcg acgcctcgcg cgcgcacggc
      601 gcccagcgcc tcggcgggct cgcgcaggtc ggtcagggcc agcgcgtagc cctcacccgc
      661 cagcacctcg gccacccgcc gaccgatccc ctgcgcggcg cccgtgacga cggcgactcg
      721 tgcggtcgaa accatcgggt gctccctaca ggcggctggc ggttggtcga cgagtggaac
      781 ggcgctgggc agaggttggc ggttcgggcg gcagggtcag gtcctgccgg cgtcggtgag
      841 ggcgtcggtg aacctgccga gcaggcgggc gaactcccgg cggtcccgct gggaccagtc
      901 gcgcatgagg cggtcgtaga gcgcctgccg ggcgcggtgc gcggcctcgg cgacctcgcg
      961 ccctgcgtcg gtcgtctcca ggtgaatgcg ccgcccgtcg gcctgggagg ccacgcgccg
     1021 caggtggccg gcccggatgg cggccgccac caggcggctg gcgcgggagg ggtcgacgcc
     1081 gagccgctcg gcgaccaggc cgacggtcac ctcctggccg ggctgctccg ggccctcgtc
     1141 gacggcgagc accgcgtcca gttgcgccag gtcgacggcg tcgccgagat cccgggtggc
     1201 gagcctgccg agggtgcgcc tggtctggct acggcggatg cggaccatcg cccgctcgat
     1261 cgcggccagg gtcgacgcgg ccgacgccat cgtgtcctgc cgcgctgtgg gtccagggcc
     1321 gctcatggcc cgcaggatac cagttgacag gtgtgttgat catctctatg ctcaagacat
     1381 ataaatgtta ttgacacatg catgctgcga gccatgaccc gtcatgcctg tggatcgtca
     1441 agacggagga tgcatcccat gaaccagcga gtgctgctgg tcggccgcag ccggcgcgtc
     1501 ctcagcaagc tcaccagcgc gctgaccgag gcgggcttcg acgcgcagtg gaccgaagac
     1561 cccgccacgg cgtcgcagcg cgtccagcca cccagcgtgg acctggtcgc cttcggccgc
     1621 ggggtcggca gccaggatcg catctcccag caggtggcca cggggatgca ctccatcccc
     1681 ctcggcaggc aggccgccgg ccagggaccc aagttcgccg tggtgcgggt cgacgccacc
     1741 gaggtctcga tcgtcaagct ctgagcctgg aaacccgagg cgatggccgg tcgggtcggc
     1801 ggtagcaggc ccgtgtccgc gggcggcaca atgacgccat ggcggcaggc gcgaccgacc
     1861 cgggcaccga ggtggtcgac ctcgtcgacg ccgaggaccg cgtcgtcggc cgcgcaccgc
     1921 gccgggtcgt ccgcagggac aagctgctgc accgcggcgt cggggtgctg tgccgcaact
     1981 cggccggcga gatctacgtg caccgccgga ccgacaccaa ggacgtcttc cccgggatgt
     2041 acgacatgct cgccggcggg gtggtcagcg cgggcgagac ctacgaggcg accgcgcgcc
     2101 gtgagatcgc cgaggagctc ggcatcgtcg ggcccgagcc gcgcctgctc ttcaagcacc
     2161 gctacaccgg cgcggacaac ccctcatgga tcgcgatctt cgaggtcgtc tgggacggtc
     2221 ccatccgtca ccaggccgag gagatcgcat ggggtgcgtt cctgaccccg gacgagctcc
     2281 tccgcaagct ggacgagtgg ccgttcgtgc ccgacagcct cgagggcttc cgcctctacc
     2341 gcgccgggaa attgccttga ccggccggcc gccgggaccg ggtcccctcc catccagcgt
     2401 tcctggtcgt cggaatcaat acgcgcgaga cgttgaacct catcgcagcg tattgaaaag
     2461 aacatgtgga tttcggcgga gcgctgcaaa agaatacggc aattgaccga ccggcgctac
     2521 ggcgtaattt gttgacacac gaccgctcca gggaatagac cggtttttgc gccggcagct
     2581 ggctcgaccg gtcgtgaccg acaacgaccg cgagccggcc gagacgccaa gtcggacttg
     2641 gcagtgctcg gtcctgactg cggcctgggg gcgggcatgg gcaagcaggt ggtcctcatc
     2701 tcggaggacc agaaactggg ctcgatcctg atgattgcgc tcgtcgaccc cggccggctc
     2761 gtgacggtcg gggcggcgag cgaactgccc gagggtggcg aggaggcaat cgaggcgatc
     2821 gtgctcgacc tgccgttgga ttcccgccgg cgcgcgtatg ccgaggtccg cgagcgcttc
     2881 gccgggcgcg tgctcgtccc ggtcgacacc gggtccgaga cgagcgactg gccgcccgac
     2941 cccgaccggc acgttctcgt gcgcccgttc cacctcgcag acctgatcgc gggcgtccag
     3001 gggccggacc ggccgcagcg gggagaggcg gagggacagc ggcggcggtc cctccgtcgc
     3061 ctccgatccg gtgtcccctg gccggtgccg gccaggggcg ccggcggggt cgcggcagcc
     3121 ccgccggcgg ccgagccggc ccccgggccg gcggcaggtc gccgcccgcg gcgggtcgcc
     3181 gggaccgcca cggccgcgct cgccgtgctc ctcgccggca tcggcgccgg catgctgctc
     3241 aagccggacc cgcctcgccc ggcgagcgaa gcgcccggcc cggtcgtgcg gcagctcgtc
     3301 gtcaccaggc cgg
//
LOCUS       DASPYY010000490         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_224287, whole genome shotgun sequence.
ACCESSION   DASPYY010000490 DASPYY010000000
VERSION     DASPYY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224287"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VEP73_07810"
     CDS             <1..320
                     /locus_tag="VEP73_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYT26367.1"
                     /translation="ELALYAEPQLDIVTFYPAVPERSCAAVDARSARLLRLAMDDPSP
                     VFLSTLRVPAERLRRRDPGLADDAASARVLRSVLMKPEHERYVDTLHAEVTRLAVRSA
                     ADA"
     gene            complement(375..632)
                     /locus_tag="VEP73_07815"
     CDS             complement(375..632)
                     /locus_tag="VEP73_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HYT26368.1"
                     /translation="MIVVTRFHGPSMALNCDLIECAEATPDTVVTLVDGTRYVIRESV
                     DEIIEKVREFRASVVVLAQQLEVEPSGGRALHAVPDADGER"
     gene            complement(810..1502)
                     /locus_tag="VEP73_07820"
     CDS             complement(810..1502)
                     /locus_tag="VEP73_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26369.1"
                     /translation="MDATALVTGGTGGLGAAVTAELLDSGWRVVVPWVEPSKLERVGR
                     RDGLELVEADLFDPGAARAAVEHAARDAHVPLRAVVNLVGGYAGGAKVADTPVEDFEQ
                     QLRLNLRPTYLVTQAALPHLVEAGGGAVVCVSSRAAVRPFPGAAGYVTAKAAVLAFAQ
                     AVAAEYKADGVRCNAILPSVIDTPANRAAQPGADHAKWVPPERVAALIRFLCSDESAA
                     TSSAAIPVYGRA"
BASE COUNT          192 a          624 c          590 g          208 t
ORIGIN      
        1 aggagctggc cctctacgcc gagccgcagc tcgacatcgt gaccttctac ccggccgtcc
       61 cggagcgctc gtgcgccgcg gtcgacgccc gctcggcgcg gctgctccgg ctggccatgg
      121 acgacccgtc cccggtgttc ctgtcgaccc tgcgcgtccc ggccgagcgc ctccgccgcc
      181 gcgaccccgg cctcgccgat gacgcggcga gcgcccgcgt cctgcgcagc gtgctgatga
      241 agccggagca cgagcggtac gtggacacgc tccacgccga ggtgacccgc ctcgccgtcc
      301 ggtcggccgc cgatgcctga ggccggtgcc aggccgcgcc ggggagcggc gctgacctgg
      361 ccgccgccgt ggggctaccg ctcgccgtcg gcgtcaggca cggcgtggag cgcccgcccg
      421 ccggacggct cgacctcgag ctgctgggcc agcacgacga cggacgcgcg gaactcgcgc
      481 accttctcga tgatctcgtc gacgctctcc ctgatcacgt acctcgtccc gtcgaccagg
      541 gtgacgacgg tgtcgggggt cgcttccgcg cactcgatca ggtcgcagtt cagggccatc
      601 gacggcccgt ggaagcgcgt cacgacgatc atgggtggtc cgtcctccgg gtcctggcgg
      661 cgcgcccgcc gccagtccct cagtcggcag cggcccctcc gacctgaggc aaccgacggg
      721 cacccggggc ggcgcgggcc gcggtggagc atgccgctcg ggacgcacgc cggcccggcc
      781 caggtgcctg gggcggcgcc cacgccgtct cacgcccgcc cgtagacggg gatcgcggca
      841 ctgctggtcg cggcggactc gtcgctgcac aggaagcgga tgagggcggc gacccgctcg
      901 ggcggcaccc acttcgcatg gtcggcgccg ggctgcgcgg cacggttggc cggcgtgtcg
      961 atcacgctcg ggaggatggc gttgcagcgc acgccgtcgg ccttgtactc ggcggcgacc
     1021 gcctgggcaa aggcgaggac ggccgccttg gcggtcacgt agccggcggc gccgggaaac
     1081 ggccgcacgg ccgccctcga cgagacgcag acgaccgcgc cgcccccggc ctcgaccaga
     1141 tgggggaggg ccgcctgggt gacgaggtag gtgggccgga ggttcagccg gagctgctgc
     1201 tcgaagtcct cgaccggggt gtcggccacc ttcgcgccac ccgcgtagcc gccgacgagg
     1261 ttgacgaccg cccggagggg gacgtgtgcg tcccgagcgg catgctccac cgcggcccgc
     1321 gccgccccgg ggtcgaacag gtcggcctcg accagctcga gcccgtcgcg ccgtcccacc
     1381 cgctcgagct tgcttggctc gacccacggg acgaccaccc gccacccgct gtccaggagc
     1441 tccgcggtga ccgcggcccc gaggccgccg gtcccgccgg tgaccagtgc ggtcgcatcc
     1501 atcctgccgt cctttccccc tgccgtcccc tccctggtcg tccttcgggt cgagtcggcc
     1561 ggcacggtgg tacggggcta ctcgagccag agcaggggcg agatcacgcg ggag
//
LOCUS       DASPYY010000491         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2752506, whole genome shotgun sequence.
ACCESSION   DASPYY010000491 DASPYY010000000
VERSION     DASPYY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752506"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1064
                     /locus_tag="VEP73_07825"
     CDS             201..1064
                     /locus_tag="VEP73_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYT26370.1"
                     /translation="MKTMWKGAISFGLVTIPVKLYGATEEKSLRFNQLHASDGGRIRY
                     KRVCALDGEEVEYSEIVKGYEYEKDHYVTLTDEELDSLPVPTAKAIEIERFVDIDQID
                     PIFFQKSYYVVPEGTGVKAYQLLREAMADDDKVALAKVSFRDKEHLATLRLRDNVFVL
                     ETMFWPDEIRAPAFDELDQEVEVRPQEVRMARSLIESLSDDFDAGEFRDEYRAALEDL
                     VARKVQGEEITYVEEEEPSKVVDLMEALRASVEAAKAGRAPPAKRAASSKPTAASKTA
                     ASRRRKKAAGE"
     gene            complement(1069..1377)
                     /locus_tag="VEP73_07830"
     CDS             complement(1069..1377)
                     /locus_tag="VEP73_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26371.1"
                     /translation="MPQAEDDHVGDHREDGDRADGQVQAALPRSPGGEGPPPGRRQHG
                     RVERRSEAERGEVGEVPDGEAGHRDREDRQRRKGRAGPGARHPGAPAGIMVGGGRQDG
                     "
     gene            1506..2450
                     /locus_tag="VEP73_07835"
     CDS             1506..2450
                     /locus_tag="VEP73_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYT26372.1"
                     /translation="MAAGQSLVWGGKAEQVGLADAARRRGVRVRLLEPGDDPRALARA
                     AVGAGADAVGVAGGDGSLGPVAAVALERDVPFVCVPTGTRNHFARDLGLDRGAPLAAL
                     DAFGGWERRVDVGMVGDRVFLNNVSLGAYADLVAEPGYRAGKLATAHAVLPARLRGEA
                     PPLAVSFHGPDGRRYSDALVLLVSNNAYELRHPLGLGLRERLDAGLLQVSALWATTGS
                     GLARLAARVRGRGTDATLAQWTATALRVDALAAGLPAGIDGEAAALVPPLEFRLLPRA
                     LRVLVPAGLARAGEADARLLGRGTVRRLWAVARDGPGG"
     gene            2654..>3455
                     /locus_tag="VEP73_07840"
     CDS             2654..>3455
                     /locus_tag="VEP73_07840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYT26373.1"
                     /translation="MKAAISLPSGPSTFDGAVATVLDHLRDRTALRLWMLTRFSGGRA
                     VVLGVRDRCYGIAAGDVLDPDVLPCTALRGGRFPRVAPAPVEAGQGHGRRLRTATHTG
                     VPVLDPEGGVFGCLCGMDPDPRPAGLPEGLPTLELLGRLLGTVLAQELEREELERRVE
                     RAELEALTDPLTGVGNRRAWDHVFDAEEARCRRYGSPASLVSVDLDGLKRVNDGRGHV
                     AGDALLRRAAQVILATKRASDVVARLGGDEFGVLAVECDEAAAAALAER"
BASE COUNT          415 a         1278 c         1308 g          454 t
ORIGIN      
        1 gtgaggcgca gctcgcggcc tgccgcctgg agcctccagg cgtcgccggc cctctcggcc
       61 ggaagggccg acgggaagcc gggccgacgt cgtttgccgc tggccacggg aggcagccct
      121 ccttgggtcg cactgctccc cagcgtacag ttgagcacgc agcacgggac cgggccgccg
      181 ccttcaccag gagcaagcgg atgaagacga tgtggaaggg cgcgatctcg ttcggcctgg
      241 tcacgatccc ggtgaagctc tacggcgcca ccgaggagaa gagcctcagg ttcaaccagc
      301 tccacgcctc cgatggcggc cgcatccgct acaagcgggt ctgcgcgctc gacggcgagg
      361 aggtggagta ctccgagatc gtcaagggct acgagtacga gaaggaccac tacgtcaccc
      421 tgaccgacga ggagctcgac tcgctgccgg tccccaccgc caaggccatc gagatcgagc
      481 gcttcgtcga tatcgaccag atcgacccca tcttcttcca gaagtcgtac tacgtcgtcc
      541 ccgaggggac cggggtcaag gcgtaccagc tgctccgcga ggccatggcc gacgacgaca
      601 aggtcgcgct ggccaaggtc tccttccgcg acaaggagca cctggccacc ctgcgcctgc
      661 gcgacaacgt cttcgtgctc gagacgatgt tctggcccga cgagatccgc gccccggcct
      721 tcgacgagct cgaccaggag gtcgaggtgc gtccccagga ggtccggatg gcccgcagcc
      781 tcatcgagag cctgtcggac gacttcgacg cgggcgagtt ccgggacgag taccgtgccg
      841 ccctcgagga cctcgtggcc aggaaggtcc agggcgagga gatcacctac gtcgaggagg
      901 aggagccgtc caaggtcgtc gacctcatgg aggccctgcg agccagcgtg gaggcggcca
      961 aggcgggcag ggcgcccccg gcgaagcggg cggcctcgag caagcccacc gcggcgtcca
     1021 agaccgcggc gtcccgccgc cgcaagaagg cggcggggga gtaggaggtc agccgtcctg
     1081 gcgccccccg ccgaccatga tgcccgcggg cgcgcccggg tggcgcgcgc ctgggccggc
     1141 tcggcccttg cggcgctggc ggtcctcgcg gtcgcggtgg ccggcttcgc cgtccggtac
     1201 ctcgccgacc tcgccgcgct cggcctcgga gcggcgctca acgcggccgt gctggcggcg
     1261 gccgggtggt gggccttcac caccaggcga gcgtggaagc gccgcctgaa cctggccgtc
     1321 ggcgcggtcg ccgtcctcgc ggtggtcgcc gacgtggtcg tcttcagcct gcggcacgtg
     1381 cgcggcgtgc tcgcgctcct ggccgtcgtc ccggtctggg ccatggccgc ccggcgggcg
     1441 ctcgccgcgg acgagcggtc ggcggcgtcg ggcgggatgc gaccgggccc gctgccggcc
     1501 agcccgtggc tgctggtcaa tccctggtct ggggtggcaa ggctgagcag gtcggcctgg
     1561 ccgacgccgc ccgccggcgc ggcgtgcggg tcaggctgct cgagccgggc gacgacccgc
     1621 gggccctggc ccgggccgcc gtgggcgccg gcgcggacgc ggtcggcgtg gccggcgggg
     1681 acggctcgct cgggccggtg gccgctgtcg cgctggagcg ggacgtgccc ttcgtctgcg
     1741 tgcccaccgg cacgcgcaac cacttcgcgc gcgacctcgg gctcgaccgc ggcgcccccc
     1801 tggccgccct ggacgcgttc ggcggctggg agcgccgcgt cgacgtcggc atggtcggcg
     1861 accgggtgtt cctgaacaac gtctcgctgg gggcgtacgc ggacctggtg gccgagcccg
     1921 gctaccgggc aggcaagctg gcgacggcgc acgccgtcct gcccgcgcgg ctccgtggcg
     1981 aggccccacc gctcgcggtc tccttccacg gcccggacgg ccgccggtac tccgacgcgc
     2041 tcgtgctgct cgtctccaac aacgcgtacg agctgcgtca cccgctgggc ctcggcctcc
     2101 gcgagcgcct ggacgccggc ctgctgcagg tgtcggcgct gtgggccacc acgggaagcg
     2161 ggctggccag gctcgccgcc cgcgtccgcg gccgggggac ggacgcgacc ctggcacagt
     2221 ggaccgccac cgcgctgcgg gtcgacgccc tggccgcggg cctgcccgcc gggatcgacg
     2281 gggaggccgc cgcgctcgtc ccgccgctcg agttccggct gctcccgcgc gcgctccgcg
     2341 tgctggtgcc cgccggcctg gcccgagccg gcgaggcaga cgcccgcctg ctcggacggg
     2401 ggaccgtgcg gcgcctgtgg gcggtcgccc gggacggccc cggcgggtga gggcgtcggg
     2461 cgccgaccgt ccggccgggc ctcctcgcca gccgggccgg ccgccctagt ccacaagcac
     2521 tccgcagttg taccgactag ccagagcggg cggtacgggg tccaatcaac aggtatcgga
     2581 ttgaccgctt cctaacgctt gcactggtca cccgatctgt ctaccctgga ctcgtcgtcg
     2641 gtggtgtggc agtgtgaagg cagcgatcag cctcccctcg ggcccgtcga ccttcgatgg
     2701 tgcggttgcc accgtcctcg accacctgcg ggaccgtacc gcgctgcggc tgtggatgct
     2761 gacgaggttc tcgggcggcc gggcggtcgt gctcggcgtg cgggaccgct gctacggcat
     2821 tgccgcgggg gacgtgctcg acccggacgt cctcccctgc acggcgctgc gaggcggccg
     2881 cttcccgcgc gtggcgcccg ccccggtcga ggccggccag gggcacggac ggcgcctgcg
     2941 gaccgccacc cacacgggcg tgccggtcct cgaccccgag ggcggcgtct tcgggtgcct
     3001 gtgcggcatg gaccccgacc cccggcccgc cgggctcccc gaggggctgc caaccctcga
     3061 gctgctcgga cggctcctcg gcaccgtcct cgcgcaggaa ctcgagcgcg aggagctcga
     3121 gcggcgcgtc gagcgggcgg agctggaggc gctcaccgac ccgctcaccg gggtcggcaa
     3181 ccggcgcgcg tgggatcacg tcttcgacgc ggaggaggcc cgctgccggc gctacgggtc
     3241 gccggcctcg ctcgtctcgg tcgatctcga cgggctgaag cgggtcaacg acgggcgcgg
     3301 gcacgtcgcg ggcgacgcgc tgctgcgccg ggccgcccag gtgatcctcg ccaccaagcg
     3361 ggcgagcgac gtggtcgcgc ggctcggcgg ggacgagttc ggggtgctcg cggtcgagtg
     3421 cgacgaggcg gccgccgcgg ccctggccga acgca
//
LOCUS       DASPYY010000492         1872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_785529, whole genome shotgun sequence.
ACCESSION   DASPYY010000492 DASPYY010000000
VERSION     DASPYY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785529"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VEP73_07845"
     CDS             complement(<1..510)
                     /locus_tag="VEP73_07845"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYT26374.1"
                     /translation="MRFFVTGGAGFIGSNYVRHLLATDPDAEVTNFDKLTYAGNPASL
                     RDIEGDPRHRFVQGDICDAAALAEVLPGHDVVVNFAAETHVDRSIVEPLAAVRTNALG
                     VAALAEAARHAGVGRFLQVGTDEEYGTIAEGSFAETDRLEPSSPYSAGEAGGSLVALA
                     YATTHKLDVV"
     gene            complement(640..1017)
                     /locus_tag="VEP73_07850"
     CDS             complement(640..1017)
                     /locus_tag="VEP73_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26375.1"
                     /translation="MVDGHEGNSFDFDAGRRGRDASHPEAPDELASGQDVAEGEATGR
                     NRGRPARADEDLFAAFMAAREAALEPGRRLSRRGWAWIAVALVLLFVVSLATGYAAYR
                     STHRLAGSGQLTAEPAPSVALPG"
     gene            1407..>1872
                     /locus_tag="VEP73_07855"
     CDS             1407..>1872
                     /locus_tag="VEP73_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HYT26376.1"
                     /translation="MLHLQDSQGSHLHGAIAEVGAALPAGVPDAAPLGPGRLAEVTRR
                     LAAAAGLWRPHVRRDAAGPVRTRLLWTPSVEVWLVEWAPGQATRAHGHDGAATALSVV
                     EGTLVEECLDATIWTTCRRTTFARASTATFGPGHVHVLANPGDGPAASVHASS"
BASE COUNT          221 a          713 c          700 g          238 t
ORIGIN      
        1 caccacgtcg agcttgtgcg tggtggcgta ggcgagggcg acgagcgagc ccccggcctc
       61 gccggcagag tagggcgagg acggctccag acggtcggtc tcggcgaacg agccctcggc
      121 gatcgtgccg tactcctcgt cggtgccgac ctggaggaag cgcccgacgc cggcgtgcct
      181 ggccgcctcg gccagcgcgg cgacgccgag ggcgttggtg cgcaccgcgg cgagcggctc
      241 gacgatggag cggtcgacat gcgtctcggc ggcgaagttg accaccacgt cgtgccctgg
      301 caggacctcg gcgagcgccg cggcgtcgca gatgtcgccc tggacgaacc tgtggcgggg
      361 gtcgccctcg atgtcacgca ggctcgcggg gttgccggcg taggtcagct tgtcgaagtt
      421 cgtcacctcg gcgtcggggt cggtcgcgag caggtggcgg acgtagttgg agccgatgaa
      481 gccggcccca ccggtcacga agaaccgcat caggtctcct ttgagatcgg ccgcggcgcc
      541 ggagaccggc cgcggcgccg gagaccggcg gcggcgcggt ggccgggcca cgggactgcg
      601 gcgaagggta gcccgtttcg cggccaggcg ccggcccgcc tacccaggga gggccaccga
      661 cggggcgggc tccgcggtga gctggccgct gcccgccagc cggtgggtgg agcggtacgc
      721 ggcgtagccg gtcgcgagcg acacgacgaa cagcaggacg agcgcgaccg cgatccaggc
      781 ccacccccgc cgggagagcc ggcggcccgg ttccagcgcc gcctcccgcg cggccatgaa
      841 cgccgcgaac aggtcctcgt cggctcgggc gggccggccg cggttccggc ccgtcgcctc
      901 gccctcggcc acgtcctgcc cggaggcgag ctcgtccggc gcttccgggt gggacgcatc
      961 ccgtcccctc ctccccgcgt cgaagtcgaa ggagttcccc tcgtggccgt cgaccatgct
     1021 cgccccgctt ccaggaccac cctgaccgtc ccgatcccgg accgtcccga tcccgggccg
     1081 atcgtatcgt ggggcacccc ggggctggaa cttgacaggg cgcgtccgtt ttcgcatcct
     1141 tcgcgcgatg gacgcatcga ccgccgctcc cgcgacccgc gacctcgccc ggacccccgg
     1201 gcggacggag tcgtgctcgt gtccgggggc tcggcgtccg gtgcgcgtcg ccagctaggc
     1261 gactcccggc cgtcgggccc gcagctcctg tctccccgcg tctgctcagc gctcccgcgc
     1321 ggcgcacccc cagtgccctc gtagggcgtc gcccaccgcc gacgccgggc gtgccctggc
     1381 cgcgactcga gcaaaggacg actctcttgt tgcacctgca agactcgcaa ggctcgcacc
     1441 tgcacggcgc catcgcggag gtcggcgccg cgctgcccgc gggcgtgccc gacgccgcgc
     1501 cgctcgggcc cgggcggctg gccgaggtga ccaggcgcct ggccgcggcg gccggcctgt
     1561 ggcgcccgca cgtccggcgg gacgccgccg gaccggtgcg gaccaggctc ctgtggaccc
     1621 cgagcgtcga ggtgtggctg gtcgagtggg cgcccgggca ggcgaccagg gcgcacggcc
     1681 acgacggcgc ggccaccgcc ctctccgtcg tggaggggac gctcgtcgag gagtgcctcg
     1741 acgcgaccat ctggaccacc tgccggcgca ccaccttcgc ccgggcgtcc accgcgacgt
     1801 tcggtcctgg ccatgtccat gtgctcgcca acccagggga cgggccggcg gcgagcgtcc
     1861 acgcgtcgtc gc
//
LOCUS       DASPYY010000493         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1769214, whole genome shotgun sequence.
ACCESSION   DASPYY010000493 DASPYY010000000
VERSION     DASPYY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769214"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VEP73_07860"
     CDS             complement(<1..823)
                     /locus_tag="VEP73_07860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HYT26377.1"
                     /translation="MTSAYERCRRLHRRHGRTYYLATLLLPAWKRRHVHALYGFTRYA
                     DEIVDDLGSDLGRAEQADALRAWGARFFAGLRGAPCDDPVLPAVLHTVRAFDLDVGDF
                     EKFLGSMATDLVRDEYATYDDLLGYMEGSAAVIGTMMTPILEASDPRRARGHARQLGL
                     AFQLTNFVRDVAEDLDRGRVYLPMEDLDRFGVRRADLAGGVVTPAVRDLLAFEVERAR
                     AHYRAAGPGIELLVPSSRPCIRTAFALYGGILDEVERAGYQVLRGRVRVPRRRRLA"
     gene            complement(949..>1537)
                     /locus_tag="VEP73_07865"
     CDS             complement(949..>1537)
                     /locus_tag="VEP73_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT26378.1"
                     /translation="LHWAPSCYLVHLGLPRRLPGQAHHTLHLGRDWTGTFEALTRHGE
                     PQPDPSLLVTHPSPEDPDAAPPGHSTLFVLEPTANTSRGHDWAALGPRVRARLYARLA
                     RLGYGDLEADATAELVVDPPAWEAMGLSAGTPFSLDHRLSQTAWLRPANASATVPGLY
                     FAGMGTVPGVGVPMVLISGRLAAERVLARRPGGGR"
BASE COUNT          191 a          551 c          627 g          168 t
ORIGIN      
        1 ccgcgagccg ccggcgcctc ggcacgcgga cgcgcccgcg cagcacctgg tacccggcgc
       61 gctcgacctc gtcgaggatg ccgccgtaga gggcgaaagc ggtccggatg caggggcggg
      121 aggagggcac gagcagctcg atgccgggcc cggcggcccg gtagtgggcc ctggcgcgct
      181 ccacctcgaa cgccagcagg tcgcggacgg cgggggtgac gacgccaccg gcgaggtcgg
      241 cccgccgcac cccgaagcgg tcgaggtcct ccatgggcag gtagacgcgg ccccggtcca
      301 ggtcctccgc cacgtccctg acgaagttgg tgagctggaa cgcgagcccc agctggcgcg
      361 cgtgcccgcg ggcacggcgg gggtcggacg cctcgaggat cggggtcatc atcgtcccga
      421 tgacggccgc cgacccctcc atgtagccga gcaggtcgtc gtaggtggcg tactcgtcgc
      481 ggaccaggtc cgtggccatc gagccgagga acttctcgaa gtcgcccacg tcgaggtcga
      541 acgcgcgcac ggtgtggagc acggcgggca gcacggggtc gtcgcagggc gcgccccgca
      601 gcccggcgaa gaagcgcgcg ccccaggccc gcagggcgtc ggcctgctct gcccggccga
      661 ggtccgagcc gaggtcgtcg acgatctcgt cggcgtagcg ggtgaagccg tacagcgcgt
      721 gcacgtggcg ccgcttccac gccggcagga gcagcgtggc caggtagtag gtcctgccgt
      781 ggcgccggtg cagccgcctg cagcgctcgt aggcggacgt cagggtgcgc ggctcggcgt
      841 cccaggccgg ccccggtgcc cgccgcgggg ccggtgccgg ccccggccgc gggacggtcg
      901 gggccgcccc ggcggccgct cgcgcctggc cgaggtcgcc ggtcgccgtc accggccgcc
      961 gccggggcgg cgggcgagga cgcgctcggc ggccagccgg cccgagatca gcaccatcgg
     1021 cacgccgacg ccgggcacgg tgcccatgcc ggcgaagtac aggccgggca cggtcgccga
     1081 ggcgttggca ggccggagcc aggccgtctg ggagagccgg tggtcgaggg agaacggcgt
     1141 gcccgccgac aggcccatcg cctcccaggc aggcgggtcg acgaccagct cggccgtggc
     1201 gtcggcctcg aggtcgccgt agccgaggcg ggcgagccgc gcgtacagcc gcgccctgac
     1261 ccgtggcccg agcgccgccc agtcgtgccc gcgcgacgtg ttcgcggtcg gctcgagcac
     1321 gaacagcgtc gagtggccgg gcggggcggc gtcggggtcc tcgggcgacg ggtgggtgac
     1381 gagcaggctc gggtcgggct ggggctcgcc gtgcctggtg agcgcctcga acgtgcccgt
     1441 ccagtcgcgg ccgaggtgga gcgtgtggtg cgcctggccc ggaagcctcc gcggcagtcc
     1501 caggtggacc aggtagcagg acggtgccca gtgcagc
//
LOCUS       DASPYY010000494         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1911880, whole genome shotgun sequence.
ACCESSION   DASPYY010000494 DASPYY010000000
VERSION     DASPYY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911880"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VEP73_07870"
     CDS             <1..212
                     /locus_tag="VEP73_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26379.1"
                     /translation="LTESATTAKTPFTITAIVNAQIGLIAPTPQKYRGKNYVFVSWSD
                     GGAQAHTITVPSANATYTATYQRTK"
     gene            complement(219..722)
                     /locus_tag="VEP73_07875"
     CDS             complement(219..722)
                     /locus_tag="VEP73_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT26380.1"
                     /translation="MEQPRASRPYMPGYGIVGPTQGGGLLPWSWAEERLTASRNYWVA
                     SSWPDGRPHAMPVWGVWHDGAFWFSSSRRSRKARNLAADPRCVVTTEHAAEPVVVEGT
                     AELVTDPAALGRMLALENAKYETGYSIELLDPAVNATIRVRPSWAFGLAEGDFTGSPT
                     RWVFEPA"
     gene            811..975
                     /locus_tag="VEP73_07880"
                     /pseudo
     CDS             811..975
                     /locus_tag="VEP73_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676578.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(998..2245)
                     /locus_tag="VEP73_07885"
     CDS             complement(998..2245)
                     /locus_tag="VEP73_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HYT26381.1"
                     /translation="MAEPATPAGRGRSARPRGRPRPREAARKWLREQVAVLRSRRLGR
                     PSWTTYRGRGAPPGWRTAKTTLAAVLAYELARQLLHSPAPPLLAPLTALLVAQLTIFE
                     TVKSGLERVGSVVAGVLVAVLLSRVVDLSWWSLAVVILASLVVGQLLHLGQHQLEVPI
                     SAMLVLAVAGQSQAAGLTRVLETLVGAVTGVVCNAVLGPPVYVQPAGDAVGTLADDMA
                     RLLEAMGEELTEGWSGEQARAWVRRTHELDRPLRSAENAVTRGEESLRLNPRRRRVLE
                     GAASLRAGLAALEHAAVQTRGLCRDLADLAEGIEERGQAEPELLVALGRLLVELGGGV
                     NAFGQLLAPDVAGPPREAVPLHIALEIARTHRDVLAELMLVDARTDPELWHIQGSLLA
                     NVDRLLREIDIERGPDARRVRRR"
BASE COUNT          327 a          915 c          825 g          287 t
ORIGIN      
        1 agctcactga gagcgcgacg acggcgaaga ccccgttcac catcaccgcg atcgtgaacg
       61 cgcagatcgg gttgatcgcc ccgacgccgc agaagtaccg ggggaagaac tacgtgttcg
      121 tgtcgtggtc cgacggcgga gcccaggcgc acaccatcac ggtgccgtcg gcgaacgcca
      181 cgtacaccgc gacctaccag cggacgaaat aggaccgctc aggccggctc gaagacccag
      241 cgggtcggcg agccggtgaa gtccccctcg gccagaccga acgcccagct cggccgcacc
      301 cggatcgtcg cgttgacggc cgggtccagc agctcgatgg agtagcccgt ctcgtacttg
      361 gcattctcca gcgcaagcat cctgccaagc gccgcggggt cggtgaccag ctcggcggtc
      421 ccctcgacga ccaccggctc cgcggcgtgc tcggtggtga ccacgcagcg cgggtcggcg
      481 gcaaggttgc gagccttgcg cgaccgccgg ctgctcgaga accagaacgc cccgtcatgc
      541 cacacgcccc agaccggcat ggcgtgcggc cgcccgtccg gccagctgct cgcgacccag
      601 tagttgcgcg aggcggtcag ccgctcttcc gcccacgacc acggcagcag gccgccgccc
      661 tgggtcgggc cgacgatgcc atagcccggc atgtacggcc ggctcgctcg tggctgctcc
      721 atgaccggcc ctccaggcga cgccgaggac aacgtggtat ggcctcgcga catcgtacgg
      781 cctcggccgc ccggggaact ctcttgctcg aaggtctcca ccgacctgcc cgacgaggtc
      841 cgcaggctca tgcgcctgta cccgcagcca cgcggccggc ggccgtcggt cgagtacatc
      901 cccgtcccct accaggcccc gcggccgcag cgcagcgagc cagcccacct gcccggctcg
      961 cagggccagc gctgagcggg gccggggtgg ggtcgactca gcggcggcgg acgcggcggg
     1021 cgtccgggcc gcgctcgatg tcgatctcgc gcagcagccg gtcgacgttg gcgagcaggc
     1081 tgccctggat gtgccacagc tccgggtcgg tccgggcgtc gaccagcatc agctcggcga
     1141 gcacgtcgcg gtgggtccgg gcgatctcga gcgcgatgtg cagcggcacc gcctcccgcg
     1201 ggggcccggc gacgtcgggc gccaggagct ggccgaaggc gttcacgccg ccgcccagct
     1261 cgaccagcag ccgcccgagc gcgaccagca gctcgggctc ggcctggccg cgctcctcga
     1321 tgccctcggc gaggtcggcc aggtcgcggc agagcccgcg ggtctggacc gccgcgtgct
     1381 ccagggcggc caggcccgcg cgcaggctcg cggcgccctc cagcacccgc cgccggcgcg
     1441 ggttcaaccg caggctctct tcccctcggg tgacggcgtt ctccgccgag cggagcggcc
     1501 ggtccagctc gtgggtgcgg cgcacccagg cgcgggcctg ctcgcccgac cagccctcgg
     1561 tcagctcctc ccccatcgcc tccagcagcc gggccatgtc gtccgcgagg gtgccgaccg
     1621 cgtcgccggc cggctgcacg tagacgggcg gcccgagcac cgcgttgcag acgacgcccg
     1681 tgacggcgcc gaccagcgtc tcgaggaccc tggtgaggcc ggcggcctgg ctctgcccgg
     1741 ccacggccag caccagcatc gcgctgatcg gcacctcgag ctgatgctgg ccgagatgca
     1801 gcagctggcc gacgaccagc gacgcgagga tcacgaccgc gaggctccac caggaaaggt
     1861 ccaccaccct cgacaggagc accgccacga gcacgccggc gacgacgctg ccgacccgct
     1921 cgagcccgct cttgaccgtc tcgaagatgg tgagctgtgc gaccagcagg gcggtcaggg
     1981 gcgcgagcag cggcggggcc gggctgtgca gcagctgcct ggcgagctcg taggcgagca
     2041 ccgcggccag cgtggtcttg gccgtccgcc agcccggcgg agcgccgcgc ccgcgatagg
     2101 tcgtccacga cggtcgaccc agccgccggg agcgcagcac ggccacctgc tcgcgcagcc
     2161 acttcctggc ggcctcgcgc ggccgggggc ggccccgggg ccgcgccgac cgcccccgcc
     2221 ccgcgggggt cgctggttct gccatccctg ttctcgtgct cctgagcatc gcgtcccaca
     2281 acctacccgg cggaggttgc atccccgtta ccggccagtc acaagtgcca tgacccgccc
     2341 gggtccgtgg tccc
//
LOCUS       DASPYY010000495         1951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2050964, whole genome shotgun sequence.
ACCESSION   DASPYY010000495 DASPYY010000000
VERSION     DASPYY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050964"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VEP73_07890"
     CDS             <1..1134
                     /locus_tag="VEP73_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HYT26382.1"
                     /translation="GTGSPQQAGPGAPATQRIGTRPNLRQLVAQVTSVPGAAVASPAA
                     GRLDLFTVGADLALYQKTLDAGVWTPSRTGPWTRLGGIVGDAPAAVVSPAPGRLDVFV
                     AGTTQAIYRKTWDGTAWHPSVTGWQWVGDPMESAPVAVSRGAGLVDLFAAGPDRALYH
                     KAWDGTAWQPSATGWERLGGAVLGTPAAVSWGVDRLDVVALGPDRRVWHRAWDQAGWQ
                     PAWEPIGGTMEGGLAAVSWGPGRFDLFAVGSDRAVYHKAWDAGAWLPSQSGWERLPST
                     VEGAPAAVSWGQDRLDLVVTGTNQNLYHKALTASGGWAPAGTWERLGGSVLATPAVGS
                     WGPGRLDVVAVGTNRALYHKAWDGTAWQPSPLTYTFLDGTVAH"
     gene            1512..>1951
                     /locus_tag="VEP73_07895"
     CDS             1512..>1951
                     /locus_tag="VEP73_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26383.1"
                     /translation="MRTTAARSTVGSSGAPTGPATPGRALFRTVLARELAATFLLLGV
                     ALAAGPARTGATPGSAPWGYQREAVDVAGRGPINAYARATIRATAWLARASIERQRDV
                     GRLNLLQYLAGDPAARAVGAWRPGHRLALEGGTAAALLALVACGV"
BASE COUNT          221 a          782 c          723 g          225 t
ORIGIN      
        1 ggcaccggct cgccgcagca ggccggccca ggcgccccgg cgacccagcg gatcggcacc
       61 cgcccgaacc tgcggcagct cgtcgcccag gtcacctccg tcccgggtgc ggccgtcgcc
      121 tcccccgccg ccggccgcct cgacctgttc acggtcggcg ccgacctggc gctctaccag
      181 aagacgctgg acgccggggt ctggaccccc tcccggacgg ggccgtggac gcggctgggc
      241 ggcatcgtcg gggacgcgcc cgccgcggtc gtctcccccg cccccggccg cctcgacgtc
      301 ttcgtggccg gcacgaccca ggcgatctac cgcaagacct gggacggcac ggcgtggcac
      361 ccgtcggtga ccgggtggca gtgggtcggc gaccccatgg agagcgcgcc cgtcgccgtg
      421 tcgcgggggg ccggcctggt ggacctgttc gccgccggcc ccgaccgggc tctgtaccac
      481 aaggcgtggg acggcaccgc ctggcagccg tcggcgaccg ggtgggagcg gctcggcggc
      541 gcggtcctcg gcacgcccgc cgccgtctcc tggggtgtgg accgcctcga cgtcgtcgcg
      601 ctcggccccg accgccgcgt gtggcaccgg gcctgggacc aggcgggctg gcagccggcg
      661 tgggagccga tcggcggcac catggagggc gggctggccg cggtctcgtg ggggcctggc
      721 cgcttcgacc tgttcgcggt cggcagcgac cgtgccgtgt accacaaggc gtgggatgcg
      781 ggcgcctggc tcccctccca gagcggctgg gagcgcctcc cgagcacggt cgagggcgcc
      841 ccggccgcgg tgtcgtgggg ccaggaccgg ctcgacctgg tcgtgaccgg gacgaaccag
      901 aacctctacc acaaggcgct gaccgcgagc ggcgggtggg cgcccgccgg cacctgggag
      961 cggctcggcg gcagcgtcct cgccaccccg gcggtgggct cgtggggccc cggccgcctc
     1021 gacgtcgtgg cggtcggcac caaccgcgcc ctctaccaca aggcatggga cggcaccgcc
     1081 tggcagccgt cgccgctgac ctacacgttc ctggacggca cggtcgccca ctgagcggcc
     1141 ggcctcctca gcacgggcag gcagacgtgg agtgccaccg cccgaggggg atgtcggtgc
     1201 gctgagcccc ggcgagcggc tccccgggcc ggcctgggct cgcggggtcc cggacgccgg
     1261 ttgacgcgga ggctgacggc gtcgacaatg atggcgaaca tatgttcgcc ggcagcccgg
     1321 cgcccgggca cttcccctgt cggtgccacc gatccatctc ggcacacgtc cacgggcccc
     1381 accgccacgc ggcgccggcc aggtccgaac gacctctcga cccgtggcat ctccggcgcc
     1441 ctggaaaccg catccccgac cagcccgcac cgcaccgtct tgagaatcgc tgcacaaggg
     1501 gaaccggagt catgcgcacg accgccgccc ggagcaccgt cgggtcctcc ggcgccccca
     1561 ccgggccggc cacgccaggc cgcgccctgt tccgcaccgt cctcgctcgc gagctcgccg
     1621 cgaccttcct gctgctcggg gtcgcgctgg ccgccggccc ggcccggacg ggcgccacac
     1681 ccggcagcgc gccatggggc taccagcgcg aggcggtcga cgtcgccggc cgcggcccga
     1741 tcaacgccta cgccagggcc accatccggg ccaccgcctg gctggccagg gcatccatcg
     1801 agcggcagcg cgacgtcggc cggctcaacc tgctccagta cctggccggc gacccggccg
     1861 ccagggcggt cggcgcctgg cggcccgggc accggctggc gctcgagggc ggcacggcgg
     1921 ccgccctgct cgcgctcgtg gcctgcgggg t
//
LOCUS       DASPYY010000496         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1067595, whole genome shotgun sequence.
ACCESSION   DASPYY010000496 DASPYY010000000
VERSION     DASPYY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067595"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VEP73_07900"
     CDS             complement(<1..511)
                     /locus_tag="VEP73_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HYT26384.1"
                     /translation="MTDPDQRAGRRWLTAWSVCRRLPEPVAFLLGWVGGRVYYQVDER
                     RRDALRSNLRHVLGPGVAPAELERVVRRGFASYGRYWIEAFRLEDLSLDEVVRRMDFE
                     GREHLDAAVASGRGVVFATPHLGNWDFGAAWLAASGYRTSAVVERLKPAALYERFVVY
                     RQALGLELLP"
     gene            complement(508..1107)
                     /locus_tag="VEP73_07905"
     CDS             complement(508..1107)
                     /locus_tag="VEP73_07905"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYT26385.1"
                     /translation="MLSSNKARNSFRHVTEPAGHALAKAGVTANSLTVVGLAGSLGAG
                     TLIAMGHDRVGGVVSLLSGLPDMLDGAVAKASGRASRRGAFLDSVVDRLSDAAVLTGI
                     VWLATLRHMPRIAVLAALVMGLSLIVSYVKARAESLGYECNAGIAERPERVIVLGVAL
                     LFGHVLAGLWVLAAGTLITVVQRVLRVWQQPELGPDIGR"
     gene            1434..>2248
                     /locus_tag="VEP73_07910"
     CDS             1434..>2248
                     /locus_tag="VEP73_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT26386.1"
                     /translation="MKSYPTDHVRNVLIVGHGGAGKTSLVEALLHATGAVNRVGRVED
                     GSTTTDFEPEETRKQISVSLALAPVEHDGYKVNLLDAPGYADFIGEVRTALPAVDAVL
                     FVVSAVDGVETQTEVVWTMAEELGLPRAFFINKLDRDRASFARTLDAIQGTFGKGCPP
                     LYLPIGEEHELGGLVGLLSGRAFTCGADGRRVEGDVPGGLADAAEELRSQLIEAVIQE
                     AEDEELMERYLGGETIAPADLIPDLEQAVAAGRLFPVLAGAATRGIGTAELLE"
BASE COUNT          337 a          800 c          843 g          268 t
ORIGIN      
        1 gcggcaggag ctccaggccg agggcctggc ggtagacgac gaagcgctcg tacagcgcgg
       61 ctggtttgag ccgctcgacg accgccgacg tccggtaccc cgaggcggcc agccaggccg
      121 cgccgaagtc ccagttgccg aggtgcgggg tggcgaagac gacgccccgg ccgctggcca
      181 cggcggcgtc caggtgctcg cggccctcga agtccatgcg ccgcacgacc tcgtcgaggc
      241 tcaggtcctc gaggcggaag gcctcgatcc agtagcggcc gtagctcgcg aacccgcggc
      301 ggacgacccg ctcgagctcg gccggggcga cgccgggccc gagcacgtgc cggaggttgg
      361 accgcagcgc gtcgcggcgg cgctcgtcga cctggtagta gacgcggccc ccgacccatc
      421 cgagcaggaa cgcgaccggc tcgggcagcc ggcggcacac gctccacgcg gtcagccagc
      481 ggcgccccgc ccgctggtcg gggtcggtca ccgaccgatg tccggcccga gctccggctg
      541 ctgccagacc cgcaggacgc gctgcaccac ggtgatgagg gtgcctgccg cgaggaccca
      601 gaggccggcg agcacgtggc cgaagagcag ggcgacgccg agcacgatga cccgctcggg
      661 gcgctcggcg atgcccgcgt tgcactcgta cccgagcgac tcggcccgcg ccttcacgta
      721 ggagacgatc agcgagaggc ccatgaccag ggcggcgagc acggcgatgc ggggcatgtg
      781 gcggagcgtc gcgagccaca cgatgccggt cagcacggcg gcgtcggaga gccggtcgac
      841 caccgagtcg aggaacgcgc cgcgccgcga ggcccggccg gacgccttgg ccaccgcgcc
      901 gtcgagcatg tcggggaggc ccgagagcag gctgaccact cccccgaccc ggtcgtggcc
      961 catcgcgatc agggtgccgg cgccgaggct gccggcgagg ccgacgacgg tcaggctgtt
     1021 cgcggtgacg ccggccttgg caagggcgtg gccggcgggt tcggtgacgt ggcggaagct
     1081 gtttctggct ttgttcgagg agagcacgga gatcctgtca acgggaaccg ccgtcccggg
     1141 cgggacggct caggggaccg gacgaccggg cgaggctagc acgccgccgg ccgccgctca
     1201 attatggcgc gatctcgttg cgaccacacc gctggatcgt ccaaatccag tggtagcatc
     1261 gcgctcccgg caggaaatgg cggtgaacgt ctgccggcag ggacgtgaac gcgagagagc
     1321 atcgccgacc acgggagtcc ggggatgagg cagctccggc ccggacccga acgactgttc
     1381 gaggggggct gtccggtccc ctcggatgca gcccgagatg gagggccgga accgtgaaga
     1441 gctacccgac cgaccacgtc cgcaacgtct tgatcgtggg ccacggtggc gccgggaaga
     1501 cctccctggt cgaggccctg ctgcacgcca ccggcgccgt caaccgggtc ggccgggtcg
     1561 aggacggctc caccacgacc gacttcgagc cggaggagac ccgcaagcag atctcggtgt
     1621 cgctggcgct cgccccggtg gagcacgacg ggtacaaggt gaacctgctc gacgcgcccg
     1681 ggtacgccga cttcatcggc gaggtccgca cggcgctgcc cgccgtcgac gccgtcctgt
     1741 tcgtcgtcag cgcggtcgac ggggtcgaga cccagaccga ggtggtctgg acgatggccg
     1801 aggagctcgg cctgccgcgc gccttcttca tcaacaagct cgaccgcgac cgcgcctcgt
     1861 tcgcccgcac cctcgacgcc atccagggga ccttcggcaa ggggtgcccg ccgctctacc
     1921 tgcccatcgg cgaggagcac gagctcggcg ggctggtcgg cctgctgtcc ggccgggcgt
     1981 tcacctgcgg cgccgacggg cgccgcgtcg agggcgacgt ccccggcggg ctcgccgacg
     2041 ccgccgagga gctgcgctcc cagctgatcg aggcggtgat ccaggaggcc gaggacgagg
     2101 agctgatgga gcgctacctc ggcggcgaga ccatcgcccc ggccgacctc atccccgacc
     2161 tcgagcaggc ggtggccgcc gggcggctgt tcccggtcct ggccggggcg gccaccaggg
     2221 gcatcggcac cgccgagctg ctcgagat
//
LOCUS       DASPYY010000497         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_926751, whole genome shotgun sequence.
ACCESSION   DASPYY010000497 DASPYY010000000
VERSION     DASPYY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926751"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VEP73_07915"
     CDS             <1..490
                     /locus_tag="VEP73_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26387.1"
                     /translation="APVEVTPVEAASRTRAGGPGQPGARTAPTAPGTPARPLPGWSPS
                     TPRRRGRRLVAALFLLAAMSVVALSATPGLVLAVDQPRGPHPVPMRESGSGSSPDGPV
                     PDFPRSAHGSGAAQAGYGLGGDCPGEDAGSVAGQDRPGDGEPDDQRDTAPGTAEDQPD
                     HR"
     gene            complement(624..1022)
                     /locus_tag="VEP73_07920"
     CDS             complement(624..1022)
                     /locus_tag="VEP73_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT26388.1"
                     /translation="MGSSLTVIELVVEDMARSLAFYRRLGLDIPAEADGQPHVEVDLP
                     GGLKLAFDTVETIRGFDSSWSPTAGSPGMSLGFRCDSPTDVDKLYGDLVAAGYEGHKE
                     PWDAAWGMRYAVLHDPDGNGVDLFCPIAAQ"
     gene            complement(1113..1796)
                     /locus_tag="VEP73_07925"
     CDS             complement(1113..1796)
                     /locus_tag="VEP73_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26389.1"
                     /translation="MRVVIAEDAALFREGLIRLLEDRGHQVCAAVADGDGLLAAVAAQ
                     HPDVAVVDIRMPPTHTDEGLRAALELRRDHPGTGVLVFSQYIETRYASRLLEGDASGV
                     GYLLKDRVADVAEFADALARVAVGGTALDPEVVGQLLRAGRHAQGLAALTPREREVLS
                     LMAEGRSNAGIASALVVSSGAVEKHVASIFGKLGLPPAEGDNRRVLAVLRFLGPDGGP
                     HQGPAHTPP"
     gene            complement(1810..>2883)
                     /locus_tag="VEP73_07930"
     CDS             complement(1810..>2883)
                     /locus_tag="VEP73_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HYT26390.1"
                     /translation="AWLLGEQVAPPPPLGPGQGPLGRLGAGFRDGHGWRAVAYLLLKL
                     PTAVFESYAVFVWAAGLINLTYPFWWGLFRNHPPDVRLRPVPVSTPFPGRVVHVGTFA
                     GTFLAFAVGAAMVLAAPWVARAVTSADRWLIRGLLGPGRLAQRVRDLEQTRALAVDDT
                     AALLRQVERDLHDGAQIRLATLAMHLGMAKEKLGEDGDAPDVTRARELVDAAHRGAKD
                     ALVELRDLAKGIHPPVLDNGLADALATLAAGSAIPVELTTDLPVRPTPAIETIAYFCA
                     AELLANAAKHSCANKIEMRAAEREGMLLLRVTDDGVGGADPAGGSGLSGLARRVATVD
                     GRLETTSPPGGPTDITAVLPLRA"
BASE COUNT          388 a         1082 c         1055 g          358 t
ORIGIN      
        1 cgcgcccgtc gaggtcacgc ccgtcgaggc cgccagccgc acccgggcgg gggggcccgg
       61 gcagcccggc gcccgcacgg caccgaccgc gcccggtaca ccggccaggc cgctgcctgg
      121 ctggtcgccc tcgacgcccc ggcgccgcgg ccggcgtctc gtcgcagcgc tcttcctcct
      181 tgccgccatg agcgtcgtcg ccctgagcgc gaccccgggg ctggtgctcg cggtcgacca
      241 accgcggggg ccgcacccgg tcccgatgcg cgaatccggg tcgggctcct ccccggacgg
      301 gcctgtgccg gacttcccga gatcagcgca tgggagcggc gccgcccagg ccgggtacgg
      361 cctgggcggc gactgccccg gggaggacgc ggggtcggtc gccgggcagg atcggccggg
      421 cgacggcgag cccgacgacc agcgggatac ggctccgggt accgccgagg accagcccga
      481 ccatcgctag gctggaggga gtcgcagcca ggcccgagcc agggcgcccg caacgccatt
      541 gcgcgcttcc ccaggccctc agcgggtcac ccggagcaca gcgtcacgct gtgctccgga
      601 ggcaccccca gacaagcggt ccgtcactga gctgctatcg gacagaacag gtccacgccg
      661 ttgccgtcgg ggtcgtggag gacggcgtac cgcatccccc aggccgcgtc ccacggctcc
      721 ttgtggccct cgtacccggc cgccacgaga tcgccgtaga gcttgtccac gtccgtgggc
      781 gagtcgcagc gaaaccccag cgacatcccc gggctcccgg cggtcggcga ccacgaggaa
      841 tcgaagccgc ggatcgtctc cacggtgtcg aaggcgagct tcaggccccc gggcaggtcc
      901 acctccacgt gcggctggcc atccgcctcg gccgggatgt cgagcccgag ccgccggtag
      961 aacgcgagcg aacgggccat gtcctctacc acgagctcga tgaccgtgag gcttgatccc
     1021 atgtcccttc ctttccagat cggccttcca cctcggcgca agatgagggc ttggaccgtg
     1081 gccgggcacg tcggcgccga ttccctgcct cgtcacggtg gagtatgggc agggccctgg
     1141 tgcgggccgc catcagggcc caggaagcgc aggaccgcga gcacgcggcg gttgtcgccc
     1201 tccgccggcg gcagtcccag cttgccgaag atgctggcca cgtgcttctc gaccgctccc
     1261 gacgagacga cgagcgcgct ggcgatgccc gcgttggacc ggccctcggc catcagggac
     1321 agcacctcgc gctcgcgcgg tgtgagcgcg gccaggccct gcgcgtgacg gccggcccgc
     1381 aggagctggc cgaccacctc ggggtcgagc gcggtgccgc ccacggcgac ccgggccagc
     1441 gcgtcagcga attcggccac gtcggcgacc cggtccttga gcaggtagcc cacgcccgag
     1501 gcgtctccct ccagcagccg ggaagcgtac ctggtctcga tgtactggga gaacaccagc
     1561 accccggtcc ccggatggtc acggcggagc tccaaggcgg cgcgcagccc ctcgtcggtg
     1621 tgcgtcggcg gcatccggat gtcgaccacc gcgacgtcgg gatgctgcgc tgccacggcg
     1681 gcgagcagcc catcgccgtc ggccacggcg gcgcatacct ggtggccgcg atcctccagc
     1741 agccggatga gcccctcacg gaacagggcc gcgtcctcgg cgatcaccac acgcatgggc
     1801 ggcatgctct catgcgcgta gcggcagcac ggcggtgatg tctgtcggcc cgcccggcgg
     1861 gctggtggtc tccaggcggc cgtccacggt ggcgacccgc cgcgcgagcc cggacaggcc
     1921 gctgccgccg gccgggtccg ccccgccgac cccgtcgtcg gtgacgcgca gcaggagcat
     1981 cccctcccgt tcggccgccc gcatctcgat cttgttcgcg cagctgtgct tggcggcgtt
     2041 ggccagcagc tcggcggcgc agaagtaggc gatcgtctcg atcgccggcg tgggccggac
     2101 cgggaggtcg gtggtcagct ccaccgggat ggcgctgccg gcggcgaggg tggcgagcgc
     2161 gtcggccagg ccgttgtcca gcaccggcgg gtggatgccc ttggccaggt cgcgtagctc
     2221 gaccagggcg tccttggcac cgcggtgggc ggcgtcgacc agttcacggg ctcgcgtgac
     2281 gtcgggcgcg tcgccgtcct cgccgagttt ctccttggcc atgccgaggt gcatcgccag
     2341 cgtggccagc cggatctgcg caccgtcgtg caggtcgcgt tcgacctggc gcagtagcgc
     2401 ggcggtatcg tcgaccgcca gcgcgcgggt ctgctccagg tcgcggaccc gctgcgcgag
     2461 cctgccgggg ccgagcaggc cccggatgag ccagcggtcg gccgaggtga ccgccctggc
     2521 cacccaggga gcggcgagca ccatcgccgc gcccaccgcg aaggccagga acgtgccggc
     2581 aaaggtgccg acatgaacca cgcggccggg gaacggggtg ctgaccggca ccggacgcag
     2641 ccgcacgtcg ggcgggtggt tgcggaacag cccccaccag aacgggtagg tcaggttgat
     2701 cagcccggcg gcccagacga agacggcgta ggactcgaac accgcggtcg gcagcttcag
     2761 cagcaggtac gccacggccc gccagccgtg gccgtcgcgg aaccccgcgc cgaggcggcc
     2821 caacgggccc tggccggggc cgagcggcgg cggcggcgcg acctgctcgc cgagcagcca
     2881 cgc
//
LOCUS       DASPYY010000498         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1348911, whole genome shotgun sequence.
ACCESSION   DASPYY010000498 DASPYY010000000
VERSION     DASPYY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348911"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1252)
                     /locus_tag="VEP73_07935"
     CDS             complement(119..1252)
                     /locus_tag="VEP73_07935"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYT26391.1"
                     /translation="MRVLVDGGSSGSGGYFRYLRGILAPGHVPPGTDVVLLCSPEVAG
                     ELREVDDRVRLLVEPVLSAPARHRRVAWWLWRYPRLVREVAPDAILHTSGFRRGRAGR
                     VPSVAVHHNMAPFSRQTFRSYGASRQTLLFLRWRLRLARSFRRADGVIFHAPYTRGEV
                     CRRVRGVKRTAIVPNAVEPRFRADAPVDRQLGPVVEILCVSSIHLFKHPWNVVEAVAR
                     LRRERSLDLRLSIVGGGEPVARARLAARIEDLRAAPWTTVAEDVPLDRMPALYRRADL
                     FVFPSAEETWPITLLEAMAAGLPIACSDRMAMPDLLRDGGVYFDPEDPAAIAAAIRTL
                     LTEAGLRRAVAAAAHRRAAAFTWDRSAAAVYRFVRAVAERPHG"
     gene            complement(1249..>2734)
                     /locus_tag="VEP73_07940"
     CDS             complement(1249..>2734)
                     /locus_tag="VEP73_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26392.1"
                     /translation="PFDRGALYAGGVALLLVVNAVVSAPDPPLPGEHWRWAAVAAAAG
                     LCLWNVSSALTAYRAGGLLSVTGFFHAWCLVAFSFPAAEMTYRYRTLRAGYWHAPTDQ
                     WLSLEAVLVLGVFELLFFLALGRDVPGAPARLARDLAARGPNFRAGLWCLVALVPLAL
                     AKLSVLSRLGVEGIVGTMITRKGYWLFLKTPESTLSFLLNAAFPVYFVSLACLATRYL
                     VRHPSPAGRRLYGAALLVSGAGVAGSGGRAELAYVLLTVLVFMHVQGYRFPADYRPVV
                     LPAVLVALTLLLVAQARHGAENPLSAASNVRNVGYDYHRGDVTQTLGVGRFDAVLMIL
                     DHAGTQPALWGKSYGYALLGAANATYGPRIALGHFLPVWRVSDQVMAPWIFGRPVASA
                     LPSAPGELLLDFGYAGVVLGALALGLAYRLATRWLYRLRGPVEVLVLLSLWILARTLS
                     DESWLMATFAASNLPLAVLLALALGRLRGNGGVEIPPRTWRRHA"
BASE COUNT          423 a          970 c         1071 g          270 t
ORIGIN      
        1 gagctgctcg atcaggcgct cttcgcggcg caggtgcgcc tgccagcccg cctcccggag
       61 gagccggagc ccgagccgcc ggaacgtggc cttcgcgagc cgcttcgcga gggcgcgctc
      121 agccatgggg ccgctccgcg acggcacgga cgaagcggta gacggccgcc gcggagcggt
      181 cccaggtgaa ggcggcggcg cgccggtggg ccgcggcggc caccgcgcgc cgcagaccgg
      241 cctcggtgag cagggtgcgg atggcagcgg cgatcgccgc ggggtcctcg gggtcgaagt
      301 agacgccgcc gtcgcgcagc aggtcgggca ttgccatgcg gtccgagcag gcgatcggca
      361 gccccgccgc catcgcctcg agcagcgtga tcggccaggt ctcctcggcc gaggggaaca
      421 cgaacaggtc ggcgcgccgg tacagcgctg gcatccggtc gagggggacg tcctcggcaa
      481 cggtcgtcca aggcgccgcc cgcaggtcct cgatgcgcgc ggcgagcctg gccctggcga
      541 ccggctcgcc gccgccgacg atcgagaggc gcagatcgag cgagcgctcc cggcgcaggc
      601 gggcgaccgc ctccacgacg ttccacgggt gcttgaacag gtggatcgac gacacgcaga
      661 ggatctcgac cacgggcccg agctgccggt cgacgggcgc gtcggcccgg aaccgcggct
      721 cgaccgcgtt cggcacgatc gcggtccgct tgacccccct gacccgccgg cacacctcac
      781 ctctcgtgta cggggcgtgg aagatgaccc cgtcggcccg gcggaagctc cgcgcgagcc
      841 gcagccgcca gcgcaggaac agcagggtct gccgcgacgc cccgtaggac cggaacgtct
      901 gccggctgaa cggcgccatg ttgtggtgga cggcgacgct cgggacgcgc ccggcccgcc
      961 cgcgccggaa cccggacgtg tgcaggatcg cgtccggcgc gacctcgcgg acgagccggg
     1021 ggtagcgcca cagccaccag gcgacccgcc ggtgcctggc cggtgccgag aggacgggct
     1081 ccaccagcag ccggacgcgg tcgtccacct cgcggagctc gccggcgacc tccggggagc
     1141 agagcaggac cacgtcggtc cctggcggga cgtggcccgg ggccaggatg ccgcgcaggt
     1201 accggaagta gcccccgctg cccgagctgc caccgtcgac gagcaccctc acgcgtgcct
     1261 cctccaggtg cggggcggga tctccacgcc gccgttcccg cggaggcggc cgagcgccag
     1321 ggccagcagg acggcgagcg gcaggttgct cgcggcgaag gtggccatga gccagctctc
     1381 gtccgagagg gtgcgggcca ggatccagag cgacagcagc accagcacct cgacggggcc
     1441 gcgcaggcgg tagagccagc gggtggcgag gcggtaggcg agcccgagcg cgagcgcgcc
     1501 gagcacgacg cccgcgtacc cgaagtcgag cagcagctcg cccggggcgg acgggagcgc
     1561 gctcgccacc ggccggccga agatccaggg cgccatgacc tggtcgctga cgcgccacac
     1621 cggcaggaag tggccgaggg cgatgcgcgg cccgtacgtc gcgttggccg cgccgagcag
     1681 cgcgtacccg tagctcttcc cccacagcgc cggttgcgtg ccggcgtggt cgaggatcat
     1741 gaggacggcg tcgaagcgcc cgacgccgag cgtctgggtg acgtccccac ggtggtagtc
     1801 gtagccgacg ttgcgcacgt tgctggctgc cgacaggggg ttctcggcgc cgtggcgggc
     1861 ctgggcgacg agcaggaggg tcagggcgac gagcacggcc ggcaggacga ccggtcggta
     1921 gtcggcgggg aaccggtagc cctggacgtg catgaacacg agcacggtga gcagcacgta
     1981 ggccagctcg gcacgcccgc cgctcccggc gacgcccgcc ccgctgacca gcagcgcggc
     2041 cccgtacagg cgacggccgg ccggcgaggg gtggcggacg agatatctgg tcgcgaggca
     2101 ggcgagcgac acgaagtaca ccgggaaggc ggcgttcagc aggaacgaca gcgtgctctc
     2161 cggcgtcttc aggaacagcc agtacccctt gcgggtgatc atcgtgccca cgatcccctc
     2221 gacgccgagc cgggagagga ccgagagctt cgcgagcgcg agcgggacga gggcgacgag
     2281 gcaccagagg ccggcccgga agttcggccc cctcgccgcg agatcgcggg ccagccgggc
     2341 gggcgccccc gggacgtccc ggccgagcgc gaggaagaac agcagctcga acacgccgag
     2401 cacgagcacc gcctcgagcg acagccactg gtcggtgggc gcgtgccagt agccggcgcg
     2461 cagcgtgcgg tagcggtagg tcatctcggc ggccgggaag gagaacgcga ccaggcacca
     2521 ggcgtggaag aacccggtga ccgacagcag gccgccggcc cggtaggcgg tgagggcgct
     2581 cgagacgttc cagaggcaga gcccggcggc ggcggcgacg gccgcccagc gccagtgctc
     2641 gccaggcagc ggcgggtcgg gcgcggagac gaccgcgttg acgacgagca gcagcgcgac
     2701 cccgccggcg tagagcgccc cgcggtcgaa gggg
//
LOCUS       DASPYY010000499         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1348932, whole genome shotgun sequence.
ACCESSION   DASPYY010000499 DASPYY010000000
VERSION     DASPYY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348932"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEP73_07945"
     CDS             <1..480
                     /locus_tag="VEP73_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT26393.1"
                     /translation="DVVLRSAAGGERRLKLAGWMRGPKKSARRPEELVVAAEWNSAGP
                     AQAFLKAGTRNAMVIAVASLALVVDRARRRVGVAMGSVGPTILSAPAAEEFARGLFEE
                     AGWENPHRPTAPAAERFGALCAQAAAPIDDVRGTAAYRRHVVAVMARRALARTAAEV"
     gene            480..938
                     /locus_tag="VEP73_07950"
     CDS             480..938
                     /locus_tag="VEP73_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT26394.1"
                     /translation="MRVSLEVNGRPLAADVWEGESLLYVLRERLGLWGSKNACEQGEC
                     GSCSVLLDGDLVCACLVLAAAADGSRVRTVEGMETDRVGRAVQRAMVEAGGVQCGFCT
                     PGFVVAIADLLKKDPHPDELAVREALAGNICRCTGYVRIFEAVQRAASDL"
     gene            947..2242
                     /locus_tag="VEP73_07955"
     CDS             947..2242
                     /locus_tag="VEP73_07955"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HYT26395.1"
                     /translation="MSGAVDGRWVLAEAGAIVEAGTGPAPAADRTLDASGCLVLPGLV
                     NCHDHMYQWATRGYAANQGLFGWLTELYPVWARIDATTVRAAARAAMARLLLSGCTLT
                     TDHHYVFPRGQAGIFEALVEAARQLGIRFQPCRGSMSLGQSAGGLPPDGVVEDHDAIL
                     VDSERLVAAYHDPAPESMCRVSIAPCSPFSVTPELMRDSAALARRHGLRLHTHLAETV
                     DEEEFCLEKFGKRPVELLEDLGWVGADVWLAHGVHFSAPEIQRLGRSRTGVAHCPSSN
                     LRLGAGACPLTELLAAGARVGLGVDGAASNEDYHLAGEVHQALLLARLRGGAGALDAH
                     SAWRLATAGGAECLGWPEVGALEPGRRADLALFRVDDLRHVGIADPLEALALAPPERA
                     QAVVVEGRVVVEDGRLLTADEDELTRDLRAAAERLRRAA"
BASE COUNT          265 a          869 c          891 g          312 t
ORIGIN      
        1 gacgtggtcc tgcgctcggc tgcgggcggc gagcgccggc tcaagctggc cggctggatg
       61 cggggcccca agaagtcggc gcgaaggccg gaggagctgg tggtggccgc cgagtggaac
      121 tccgcgggac cggctcaggc cttcctgaag gcgggcaccc gcaacgcgat ggtgatcgcg
      181 gtcgccagcc tggccctggt ggtcgaccgc gcccgccgcc gggtgggcgt ggccatgggc
      241 tcggtgggtc cgacgatcct gagcgcgccc gcggccgagg agttcgcccg cggcctgttc
      301 gaagaggcgg ggtgggagaa cccgcatcgc cccaccgcgc ccgcggccga gcgcttcggc
      361 gcgctctgcg cgcaggcggc cgcgcccatc gacgacgtcc gggggacggc ggcctaccgc
      421 cgccacgtgg tggcggtgat ggcgcgccgg gccctggcca ggaccgccgc cgaagtttga
      481 tgcgggtatc cctggaggtc aacggccgcc ccctggcggc ggacgtctgg gagggcgagt
      541 cgctgctcta cgtgctccgg gagcggctcg ggctgtgggg cagcaagaac gcctgcgagc
      601 agggcgagtg cggctcctgc agcgtgctcc tggacgggga cctggtctgc gcgtgcctgg
      661 tccttgccgc cgccgccgac gggtcgcggg tgcggacggt cgagggcatg gagaccgacc
      721 gcgtcggccg cgccgtgcag cgggcgatgg tcgaggccgg tggcgtccag tgcggtttct
      781 gcactccggg gttcgtcgtg gccatcgccg acctgctgaa gaaagacccg catcccgacg
      841 agctggcggt ccgggaggcg ctggccggca acatctgccg ctgcaccggc tacgtgcgca
      901 tcttcgaggc cgtgcagcgc gcggcctccg acctttgaag ctgctggtga gcggcgccgt
      961 cgacggccgc tgggtgctgg ccgaggccgg cgccatcgtc gaggccggca ccggcccggc
     1021 gccggccgcc gaccgcacgc tcgacgcctc cggctgcctg gtgctgcccg ggctggtcaa
     1081 ctgccacgac cacatgtacc aatgggcgac gcgcggctac gccgccaacc agggcctctt
     1141 cggctggctg accgagctct acccggtctg ggcgcgcatc gacgccacca ccgtgcgcgc
     1201 ggcggcccgc gcggccatgg cccgcctcct gctcagcggc tgcaccctga ccaccgacca
     1261 ccactacgtc ttcccgcgcg gccaggccgg gatcttcgag gccctggtgg aggcggctcg
     1321 ccagctcggc atccgcttcc agccctgccg cggctcgatg tcgctcggcc agtcggcggg
     1381 cggactgccg ccggacgggg tggtcgagga ccacgacgcc atcctggtcg acagcgagcg
     1441 cctggtcgcc gcctaccacg acccggcgcc ggagtcgatg tgccgggtct cgatcgcgcc
     1501 ctgctcgccc ttctcggtga cccccgagct gatgcgcgac tcggccgccc tggcccgccg
     1561 ccacggcctg cgcctgcaca cccacctcgc ggagaccgtc gacgaagagg agttctgcct
     1621 ggagaagttc ggcaagcgcc ccgtcgagct gctcgaggac cttggctggg tgggggccga
     1681 cgtctggctg gcccacggcg tccacttctc ggcgcccgag atccagcgcc tgggccgctc
     1741 ccgcaccggg gtcgcgcact gcccgtcctc caacttgcgg ctgggcgccg gcgcctgccc
     1801 gctgaccgag ctgctggccg ccggcgcgcg ggtcggcctg ggggtcgacg gggcggccag
     1861 caacgaggac taccacctgg ccggcgaggt ccaccaggca ctgctgctgg cccggctgcg
     1921 aggcggcgcc ggggcgctcg atgcgcactc cgcgtggcgc ctcgccaccg cgggtggggc
     1981 cgagtgcctg ggctggccgg aggtgggtgc gctggagccc ggccgccgcg ccgacctggc
     2041 gctcttccgg gtcgacgacc tgcgccacgt gggcatcgcc gatccgctgg aggccctggc
     2101 gctggcgccc ccggagcgcg cccaggccgt cgtcgtagaa gggcgggtgg tcgtcgaaga
     2161 cggtcgcctg ctcaccgccg acgaggacga gctcacccgc gacctgcgcg cggccgccga
     2221 gcgcctcagg cgagccgcct gacgtgacgt ctccgggacg atcgacgcat cttcggcgcc
     2281 cagggtgccg attcgcgcgt cgcggggccc ggcccagcgt cgtcacgcag ctcaggc
//
LOCUS       DASPYY010000500         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_786601, whole genome shotgun sequence.
ACCESSION   DASPYY010000500 DASPYY010000000
VERSION     DASPYY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786601"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VEP73_07960"
     CDS             complement(<1..1209)
                     /locus_tag="VEP73_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT26396.1"
                     /translation="MAELSRPVGLGLAARGSAADTVAWAGQARRAGLDSVWIHDSYFE
                     RDAVTYASAIAAQVPGIRVAMGALNPLTRHPVLVAMTVSAIDDMAPGRVILGMGTGLP
                     LRLAQMGIPYTPEAGVEAVSRAIDTMRALWRGERVPAAAPGLPPVQPMFPPVHRVPVY
                     VAAYRSAFLELAGQKADGYLARPAESIPSLARMLEKLRAASVAAGRPRDAVDVAGYLL
                     TLVDRTRREALNRAKREPFVIYMMSVQSDLAMRRAGLDPELKDRIAAAWRAEDYHRAA
                     GLIPDELLDAFMLCGTRDEVASRAADYHEAGMTRPVLQPVVQEEEHVRDVLAAAVEYG
                     VAAAAPPGRGAPTGPSAGAARGPGRPGLDEQRLGIGARIARRVAAWGEIARPFSFTAS
                     VVPVAAAGALA"
     gene            complement(1301..2437)
                     /locus_tag="VEP73_07965"
     CDS             complement(1301..2437)
                     /locus_tag="VEP73_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYT26397.1"
                     /translation="MARDRDGDRLNLLVQHPRARIGRRGFLRVSALLGAAGLAGCTTG
                     RACPAQPAGNRVQEQLLNIFNWSDYIDDRSIPAFQAHSGIRVNYDVYSSNDDLLAKMK
                     AGHNQLDIIVPTNWFIPTYRRLGLIEPLRRDLIPNMRNLDRAFVDTDYDHGNQYTVPW
                     QWGTTGIGYNVTKVPGGKIDSWKSLFDPAPQVSGRVSLLREASELINCALIYLGKAPN
                     SSADADLAEVVSLIRSLKGRISKFSSDTYIDELAKNQTWIAHGWSGDVAQATDRNRNV
                     AYAIPKEGSLRWADVMCVPKGAPHPKNAALFMNYVLQPTVNARISKYVSYGTPVTLAK
                     ALLPDEQLKNPAIYPPASVKLSVITPGGEKLQKWQAAFDRIVKG"
     gene            complement(2514..3125)
                     /locus_tag="VEP73_07970"
     CDS             complement(2514..3125)
                     /locus_tag="VEP73_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26398.1"
                     /translation="MGVLGLVVALLVVGVPLARMIGKAQQTPSRTGSDASVPPASGST
                     APPQGPVPTAVGNLLSNWSFEQDLSGWQGVGPVSVVRELGGRTSGSSAFVQATTNGRT
                     PVGIVAASVVPEARAGVVYEASVWVRSGTPGLEVALSLVVTHGGTKEASRTVAETVSD
                     PRWLRVDVAHRVTAPGTLAVQVTADGLSQGEGFLVDEVTVRQA"
BASE COUNT          504 a         1356 c         1339 g          522 t
ORIGIN      
        1 ggccagggcg ccggccgccg ccaccgggac gaccgaggcg gtgaagctga acggcctggc
       61 gatctcgccc caggccgcga cccgcctggc gatccgcgcc ccgatcccca ggcgctgctc
      121 gtcgaggccg ggacggccgg ggccgcgggc cgcccctgcg gagggaccgg tcggcgcccc
      181 ccggccgggt ggtgcggcgg ccgccacgcc gtattccacc gcggcggcca ggacgtcgcg
      241 cacgtgctcc tcctcctgca cgaccggctg gagtaccggc cgggtcatgc ccgcctcgtg
      301 gtagtcggcg gcccgggagg ccacctcgtc ccgggtgccg cagagcatga acgcgtcgag
      361 cagctcgtcc gggatcagcc cggccgcgcg gtggtagtcc tcggcccgcc aggccgccgc
      421 gatgcggtcc ttcagctcgg ggtcgagccc ggcccggcgc atggccaggt cggactgcac
      481 gctcatcatg tagatcacga acggctcgcg cttggcccgg ttcagcgcct ccctgcgggt
      541 ccggtcgacc agcgtgagca ggtagccggc cacgtcgacg gcgtcccgcg ggcggccggc
      601 ggccaccgag gcggcccgca gcttctcgag catcctcgcg agcgacggaa tcgactcggc
      661 cggccgggcg aggtacccgt cggccttctg cccggccagc tccaggaacg ccgaccggta
      721 ggcggcgaca tagacgggca cccggtgcac cggcgggaac atcggctgca cggggggcag
      781 cccgggggcc gcggccggga cccgctcccc cctccacaac gcccgcatgg tgtcgatcgc
      841 ccggctgacc gcctccaccc ctgcctccgg ggtgtagggg atccccatct gggccaggcg
      901 gagcggcagc ccggtgccca tgccgaggat cacccggccg ggcgccatgt cgtcgatggc
      961 gctcaccgtc atcgcgacca gcaccggatg cctggtgagc gggttgagcg cgcccatcgc
     1021 gacccggatg cccggcacct gggcggcgat cgccgaggcg taggtgaccg cgtcacgctc
     1081 gaagtagctg tcgtggatcc acaccgagtc cagcccggcg cgcctggcct gcccggccca
     1141 ggccacggtg tcggccgcgc tgccgcgggc ggccagtccc agcccgaccg gcctcgagag
     1201 ctcggccatg ttctttgtgc cctccttcac aagcgctgct gcaccagcgt acgacagttg
     1261 accacgagac gcctccagga cgcggcgcgc ctggcggctc tcagcccttg acgatgcggt
     1321 cgaaggccgc ctgccacttc tgcagcttct cgccgcccgg ggtgatgacc gagagcttca
     1381 ccgaggcagg cgggtagatg gccgggttct tgagctgctc gtcggggagc agcgccttgg
     1441 ccagggtcac cggcgtgccg taggagacgt acttcgagat acgggcgttg accgtcggct
     1501 gcagcacgta gttcatgaac agggcggcgt tcttgggatg gggcgcgccc ttcgggacgc
     1561 acatcacgtc cgcccagcgc agcgagccct ccttggggat cgcgtaggcg acgttccggt
     1621 tacggtcggt cgcctgcgcc acgtcgccgc tccacccgtg cgcgatccag gtctggttct
     1681 tggccagctc gtcgatgtag gtgtcgctcg agaacttgct gatcctgccc ttgagcgacc
     1741 tgatgagcga gacgacctcg gccaggtcgg cgtcggcgct cgagttcggg gccttgccga
     1801 ggtagatcag cgcgcagttg atcagctcgc tcgcctcgcg gagcagcgag acccgcccgg
     1861 agacctgggg ggcggggtcg aacagcgact tccaggagtc gatcttcccg ccggggacct
     1921 tggtgacgtt gtacccgatc ccggtcgtcc cccactgcca ggggaccgtg tactggttgc
     1981 cgtggtcgta gtcggtgtcc acgaacgccc ggtcgaggtt gcgcatgttc gggatgaggt
     2041 cgcggcggag cggctcgatc aggccgaggc ggcggtaggt ggggatgaac cagttggtgg
     2101 ggacgatgat gtcgagctgg ttgtgcccgg ccttcatctt ggccagcagg tcgtcgttgg
     2161 aggagtagac gtcgtagttc acccggatgc ccgagtgggc ctggaacgcg gggatgctcc
     2221 ggtcgtcgat gtagtcgctc cagttgaaga tgttcaggag ctgctcctgc acgcgattcc
     2281 cggccggctg ggcggggcac gccctgcccg tcgtacagcc ggcgaggccg gccgccccga
     2341 gcagcgcgct cacccgcagg aagccgcgcc tgccgatgcg cgcccggggg tgctgcacca
     2401 gcaggttgag ccgatctccg tcgcggtccc ttgccactcc agcctcctcg ctccacattg
     2461 gccggctcga cgcgccgcca gcctcacccg cccgtcatct gccggccgga gcgctacgcc
     2521 tgacggacgg tgacctcgtc gaccaggaac ccctcaccct gcgagaggcc atcggccgtc
     2581 acctggacgg cgagggtgcc cggcgcggtg acgcggtggg cgacgtccac ccggagccaa
     2641 cggggatccg acaccgtctc ggcgacggtc cgggatgcct ccttcgtccc cccgtgggtg
     2701 acgacgaggc tcagcgccac ctcgagtccc ggggtgcccg accggaccca gaccgaggcc
     2761 tcgtacacga cgccggcccg cgcctccggg accacggacg cggccacgat gcccaccggg
     2821 gtccgcccgt tcgtggtcgc ctggacgaac gcgctcgacc cggaggtgcg gccgcccagc
     2881 tcgcggacca cgctcaccgg cccgacgccc tgccagccgg acaggtcctg ctcgaagctc
     2941 cagttgctga gcaggttgcc gacggccgtg gggaccggcc cctgcggcgg cgcggtggag
     3001 ccgcttgccg gcgggacgct cgcatccgac cccgttctcg agggggtctg ctgcgccttg
     3061 ccgatcatgc gggcgagcgg caccccgacc accagcagcg cgacgacgag cccgagcacg
     3121 cccatgaact ggcgtacccc ggtgcggggc ctgtccggcc tgggtgcggc gtgccggccg
     3181 ccgcggggcg cgccatcgtc cgtctcccct ccgggatcga ggccaggggc gggctggcgg
     3241 ttccacgagg ccgaacgggg gggttcgggg gtacgccgtg cgccgggggg attgtcgacg
     3301 ttgcccattg aggccacctg gatcacggac cggggaaacc ggacactaat cgagcgctca
     3361 ggctgattgc aagaaaacga gactctcggt cctaccagcg aaatgcgatg aagctacgcc
     3421 cagcctccct gctcgggacg cgagggtggc ttgggtgtgg cgactacgct ggagcggacc
     3481 gcgccgccgg ctcacgcccg gctccgcccg ccgacctgcc agaggccgac ctgccagagg
     3541 aggacgaccg atgccgaggc cagccggaac caccaccgag atcgcccgcg tccccgagcc
     3601 ggtcaacgag cccgtccaca cctatgcgcc cgggtcgccc gagcggggcc ggctcgagca
     3661 gcgcctggcc gccatggcca aggagcggat cgacgcgccc atgctcatcg gcggggccgc
     3721 c
//
LOCUS       DASPYY010000501         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_225377, whole genome shotgun sequence.
ACCESSION   DASPYY010000501 DASPYY010000000
VERSION     DASPYY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225377"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /gene="mreD"
                     /locus_tag="VEP73_07975"
     CDS             complement(<1..315)
                     /gene="mreD"
                     /locus_tag="VEP73_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYT26399.1"
                     /translation="MRRVLALAAVIVVAVLLESTLLGRMRLGGVQPDLLVLTVVAVAM
                     ASGPTSGAVFGFWAGLVADLLFAPPVGVSALVYTAVGYATGLVRVYVVSLSAWVHLAL
                     AGA"
     gene            complement(318..1190)
                     /gene="mreC"
                     /locus_tag="VEP73_07980"
     CDS             complement(318..1190)
                     /gene="mreC"
                     /locus_tag="VEP73_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYT26400.1"
                     /translation="MHDHPRRARIILAVLLLASVTVITVDFRESAGGPVDRLQRVAVG
                     LFGPLQQGVSAVVRPVGGFFGALTQVGGLRGDNRRLKAEVDRLRAQERTYQDLLTEND
                     QLRGTLNMARRCGCRTVGATVVARSGSSFQWSVTIDAGSRQGIAPDMAVVDAQGLVGR
                     VVAVAGGYSTVLLIVDPGSGVAASLARTRTAGLLRGGGSGPLGFEPVQAGTQVKVGDA
                     VVSQGYQGGIFPQGIPIGVVERVGPTAGGLVQTVAVRPYADVATLDVVAVVVGRPPVP
                     PPPAGSARTGAGGR"
     gene            complement(1248..2279)
                     /locus_tag="VEP73_07985"
     CDS             complement(1248..2279)
                     /locus_tag="VEP73_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYT26401.1"
                     /translation="MQNAFRFLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTRN
                     NAILAVGADAKAMIGRTPGHIVAIRPLRDGVIADFDTTEKMLRYFVHKVHRRQFMAKP
                     RVVVCVPSGITGVERRAVFDSTISAGARGAFIIEEPMAAAIGVGLPVHEPTGNMVVDI
                     GGGTTEVAVISLGGIVTSQSIRIGGDELDEAIISYVKKEYSLMLGERTAEEIKITIGS
                     AYPLPEEPRTEIRGRDLVTGLPKTITVGAEEIRKAIEEPVNAVVDAVKNTLDHTPPEL
                     AADVMDKGIVLTGGGALLRGLDERLKHETSMPIHLADDPLQSVAIGSGKCVEEFESLK
                     RVLIASSRR"
     gene            complement(2471..2911)
                     /gene="ndk"
                     /locus_tag="VEP73_07990"
     CDS             complement(2471..2911)
                     /gene="ndk"
                     /locus_tag="VEP73_07990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYT26402.1"
                     /translation="MPNATPNETPNERTLVLCKPDAVARGLVGEVVTRLERKGFRLVA
                     MDLRVLDEATAKQHYAEHEGKPFFEGLVSFITSGPLVAICVEGPEAVVAVRTLMGPTN
                     PVAAPPGSIRGDYALLIGQNLVHGSDSPASAARELALFFPELSG"
BASE COUNT          418 a         1076 c         1091 g          382 t
ORIGIN      
        1 cgccccggcg agcgccaggt gcacccacgc gctcaggctg accacgtaca cccgcacgag
       61 gcccgtggcg tagcccacgg cggtgtagac gagcgcggag accccgaccg gcggcgcgaa
      121 cagcaggtcg gcgacgagcc cggcccagaa gccgaacacg gcgcccgagg tgggccccga
      181 ggccatggcg accgccacca cggtgaggac cagcaggtcg ggctggaccc cgccgagccg
      241 catgcggccc agcagggtcg actcgagcag cacggcgacc acgatgacgg cggcgagggc
      301 gaggacccgg cgcaccgcta ccgccccccg gccccggtcc gcgccgagcc ggcgggcggc
      361 gggggcaccg gcggcctgcc caccaccacg gcgacgacgt cgagcgtggc gacgtcggcg
      421 tacggccgca ccgcgacggt ctgcaccagg ccgcccgcgg tcgggccgac ccgctcgacc
      481 acgccgatcg ggatcccctg cgggaagatc ccgccctggt agccctggct gaccaccgcg
      541 tcgcccacct tgacctgggt gccggcctgg accggctcga acccgagcgg gccgctgccg
      601 ccgccgcgca gcaggccggc ggtcctggtg cgggcgaggc tcgcggcgac cccgctgccg
      661 gggtcgacga tcagcaggac ggtcgagtag ccgccggcca cggcgacgac ccggccgacg
      721 agcccctgcg cgtcgacgac ggccatgtcg ggggcgatcc cctgccgcga gcccgcgtcg
      781 atcgtgaccg accactggaa gctcgagccc gagcgggcca ccacggtggc cccgaccgtc
      841 cggcagccgc accgcctcgc catgttgagg gtgccccgga gctggtcgtt ctcggtgagc
      901 aggtcctggt aggtgcgctc ctgggcgcgg agccggtcga cctcggcctt gaggcgccgg
      961 ttgtcgccgc gcaggccgcc gacctgcgtc agggcgccga agaacccgcc gaccggacgg
     1021 acgaccgcgc tcaccccctg ctgcagcggc ccgaacaggc cgacggcgac ccgctgcagc
     1081 cggtccacgg gaccgccggc cgactcgcgg aagtcgaccg tgatcaccgt gaccgaggcg
     1141 agcagcagca cggcgaggat gatgcgggcg cggcgcgggt gatcgtgcaa cgtcagtcag
     1201 gaaggcggct tgcctcccag cctccagggg acgggccagg ggacgggtca acggcgcgag
     1261 gacgcgatga gcacgcgttt cagggactcg aactcctcca cgcacttgcc cgacccgatg
     1321 gccaccgact gcagcgggtc gtcggccagg tggatcggca tgctggtctc gtgcttgagg
     1381 cgctcgtcga ggccccgcag cagcgcgccc ccgccggtga ggacgatgcc cttgtccatc
     1441 acgtcggcgg ccagctcggg cggggtgtgg tcgagggtgt tcttgacggc gtcgacgacc
     1501 gcgttgaccg gctcctcgat cgccttgcgg atctcctcgg cccccaccgt gatcgtcttg
     1561 ggcaggccgg tgacgaggtc gcggccgcgg atctcggtcc gcggctcctc gggcagcggg
     1621 taggccgagc cgatcgtgat cttgatctcc tcggcggtgc gctcccccag catcagggag
     1681 tactccttct tcacgtagct gatgatcgcc tcgtcgagct cgtcgccgcc gatccggatc
     1741 gactggctgg tgacgatgcc gccgagggag atgacggcca cctcggtggt gcccccgccg
     1801 atgtcgacca ccatgttgcc ggtcggctcg tgcacgggca gccccacgcc gatcgcggcc
     1861 gccatcggct cctcgatgat gaacgccccc cttgcgccgg cgctgatggt cgagtcgaag
     1921 accgcgcgcc gctcgacgcc ggtgatgccg gacgggacgc acacgaccac cctcggcttg
     1981 gccatgaact ggcgccggtg caccttgtgc acgaagtagc ggagcatctt ctcggtggtg
     2041 tcgaagtcgg cgatgacccc gtcgcgcagc ggccggatcg ccacgatgtg gccgggggtg
     2101 cggccgatca tcgccttggc gtcggccccg acggccagga tcgcgttgtt cctggtgttg
     2161 atggcgacca ccgacggctc gttgagcacg atcccccggc cccggacgta gacgagcgtg
     2221 ttggccgtcc ccaggtcgac cgccatgtcg cggccgagga aacggaaggc gttctgcaac
     2281 ggaaccagcc ccttcgagcg cttgcagacc ccgagtgcgg cagggcacgc gggagcaccg
     2341 gacggcgtgg cggtccccgc gacgcggccc ggctcggtcg agagcccgtg catcctacat
     2401 cagcccctcg aactccccga cagcgtcggt cggctcgatg tggctgggcg ggacgcctga
     2461 tgccactggt ttaccccgac agctccggga agaacagcgc cagctcacgg gcggccgacg
     2521 ccggcgagtc cgatccatgc accagattct gcccgatcag gagagcgtag tcgccccgga
     2581 tcgagccggg gggcgcggcg accgggttcg tcgggcccat gagcgtgcgc acggcgacga
     2641 ccgcctccgg cccctcgacg cagatcgcga ccagggggcc gctggtgatg aacgacacga
     2701 gcccctcgaa gaagggcttg ccctcgtgct cggcgtagtg ctgcttggcg gtcgcctcgt
     2761 caaggacgcg caggtccatc gcgacgaggc ggaagccctt gcgctcgagg cgggtcacca
     2821 cctcgccgac caggccgcgg gcgacggcgt cgggcttgca gaggaccagg gtgcgttcgt
     2881 tgggcgtctc gttgggcgtc gcgttgggca tcgtggacct ccggcggagc ggcgggcggg
     2941 cgcggttggc gggcggcgga cggcggg
//
LOCUS       DASPYY010000502         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2612920, whole genome shotgun sequence.
ACCESSION   DASPYY010000502 DASPYY010000000
VERSION     DASPYY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612920"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VEP73_07995"
     CDS             complement(<1..979)
                     /locus_tag="VEP73_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYT26403.1"
                     /translation="MSQPSRLDELVRTARIMICCGSGGVGKTSTAAAVAVRAAGRGRR
                     TCVLTIDPARRLAQALGLDLLSNTPKPVTGRGLPQDGPGSLDAMMLDMRRTFDEVIER
                     YARDADQADRILANRFYQRVSSTLAGTQEYMAMEKLYELHESHRYDLLVVDTPPTRSA
                     LDFLDAPDNLNELLDARAFRLLIAPAQRIGRGYLRGLNLATTAMTKMVRRLTGSDLLA
                     EVGEFFTAFEGMYDGFRDRAQRVYDLMKHPSTAFVVVATPDEAALREAAFFAGRLAED
                     RMPLGALVVNRVHRAGHVPAVPPSARQRLAAGGSDARLLADLLDQHGALE"
     gene            complement(976..1986)
                     /locus_tag="VEP73_08000"
     CDS             complement(976..1986)
                     /locus_tag="VEP73_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYT26404.1"
                     /translation="MSFDTLDDARLLVVTGKGGTGKTTVAAALAVAAAARGRRVLVVE
                     VEGRQGLAGLFGRRSLSYEETRVAARVAALAVDSEESLREYLGRFGAAPLARLLTWAR
                     LTRFITAAAPGLGDVLLVGKVWEAATRVRGGRPVYDLVVLDAPPTGRVVPFLRAPETV
                     AELARVGPIRHQADQVKDLLDDRGRTRVVFTCLPEELPVTETLEGVGLLGGAGLPLGP
                     IVVNQVVADRLGSRARRLAALVKDPGPLAVAAKEAGASIDDDALGVLLGEARDRQRVV
                     AAQRRLIAELRAGAPELPVVELPLLLDGAAGPDAVGDLARVLAAADLATRRAPATAPA
                     RT"
     gene            2085..>2552
                     /locus_tag="VEP73_08005"
     CDS             2085..>2552
                     /locus_tag="VEP73_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26405.1"
                     /translation="MRRIPPLSCLLALAALLAATFAAPASAGTPGTARSAPAGPSVEV
                     FEASGVIDPSLLGALRRDLASADRRGVEVFLIQLDSFGSLDVDPADLQRVLAGARVPV
                     AVWLGPRSARAGGAAAFLLAAADVVAVSRQARVGPALPAELGRGRDPAAEQALF"
BASE COUNT          286 a          963 c         1001 g          302 t
ORIGIN      
        1 cctcgagcgc cccatgctgg tcgagcaggt cggcgagcag ccgggcgtcg gagccgccgg
       61 ccgcgagccg ctgccgcgcg gagggcggga cggccggcac gtgcccggcc cggtggacgc
      121 ggttgacgac gagcgccccg agcggcatgc ggtcctcggc caggcggccg gcgaagaacg
      181 cggcctcgcg cagggccgcc tcgtcggggg tcgccaccac cacgaacgcg gtgctcgggt
      241 gcttcatgag gtcgtagacc cgctgggcgc ggtccctgaa gccgtcgtac atgccctcga
      301 acgcggtgaa gaactcgccg acctcggcga gcaggtccga gccggtgagc cggcgcacca
      361 tcttcgtcat cgcggtcgtc gccaggttca gcccgcgcag gtagccgcgc ccgatgcgct
      421 gtgcgggggc gatgagcagg cggaaggcgc gggcgtcgag cagctcgttg aggttgtcgg
      481 gggcgtcgag gaagtcgagc gccgagcggg tgggcggcgt gtccaccacg aggaggtcgt
      541 agcggtgcga ctcgtgcagc tcgtacagct tctccatcgc catgtactcc tgggtgccgg
      601 ccagggtgga cgagacccgc tggtagaagc ggttggccag gatccggtcg gcctggtcgg
      661 cgtcgcgcgc gtagcgctcg atgacctcgt cgaacgtgcg ccgcatgtcg agcatcatgg
      721 cgtcgaggct gccagggccg tcctgcggca gccccctgcc cgtgaccggc ttgggggtgt
      781 tggacagcag gtcgaggccg agcgcctgcg cgagccggcg ggccgggtcg atcgtcagca
      841 cgcaggtccg ccgtccgcgc ccggcggccc ggacggccac ggcggccgcg gtcgaggtct
      901 tgcccacccc gccggagccg cagcagatca tgatccgggc ggtgcgcacc agctcgtcca
      961 ggcgggaggg ctggctcatg tccgcgccgg cgcggtcgcc ggcgcccgcc gggtggcgag
     1021 gtcggccgcg gcgaggaccc tggccaggtc gccgaccgcg tccgggccgg ccgccccgtc
     1081 caggagcagc gggagctcca cgaccggcag ctcgggcgcg ccggcgcgga gctcggcgat
     1141 caggcggcgc tgcgcggcga ccacgcgctg gcggtcccgc gcctcgccga gcaggacgcc
     1201 gagggcgtcg tcgtcgatgc tcgcgcccgc ctccttcgcc gccaccgcga gcggcccggg
     1261 gtccttgacc agggccgcga gccggcgtgc ccggctgccc agccggtcgg cgaccacctg
     1321 gttgaccacg atcgggccga gcggcaggcc ggccccgccg agcagcccga cgccctccag
     1381 ggtctcggtg accggcagct cctcgggcag gcaggtgaac acgacccgcg tgcgcccccg
     1441 gtcgtcgagc aggtccttga cctggtcggc ctggtgccgg atggggccca cccgggccag
     1501 ctcggccacg gtctcgggcg cgcgcaggaa ggggacgacc cggccggtgg gcggggcgtc
     1561 gagcaccacg aggtcgtaca cggggcggcc gccccggacg cgcgtggccg cctcccacac
     1621 cttgccgacg agcagcacgt cgccgagccc gggggcggcg gcggtgatga accgggtgag
     1681 cctcgcccag gtcagcaggc gggcgagcgg cgcggcgccg aaccggccga ggtactcgcg
     1741 gagggactcc tcggagtcga ccgcgagggc ggcgacccgg gcggcgaccc gcgtctcctc
     1801 gtaggagagc gaccgccggc cgaacagccc ggcgagcccc tgccggccct cgacctcgac
     1861 gaccaggacg cgccgcccgc gcgccgccgc ggccaccgcg agcgcggcgg cgaccgtggt
     1921 cttgcctgtg ccgcccttgc cggtcaccac cagcagccgg gcgtcgtcga gggtgtcgaa
     1981 ggacatgacc gccatcgtag ccgcccccga tgccgccgtc cgagacgcct cggcccggtg
     2041 cggcgtggcg cgccgggcgc ctggcgggga agaatggagg cgccatgcgt cgcatcccgc
     2101 ccctctcctg cctgctcgcc ctggccgcgc tgctcgccgc gaccttcgcc gctcccgcct
     2161 cggccgggac gcccgggacc gctcggtccg cccccgccgg tccgagcgtg gaggtgttcg
     2221 aggcgtcggg cgtgatcgac cccagcctgc tcggtgcgtt gcgccgcgac ctggcgagcg
     2281 cggaccggcg cggcgtcgag gtgttcctga tccagctcga cagcttcggc agcctcgacg
     2341 tcgacccggc cgacctccag cgggtgctgg ccggcgccag ggtcccggtg gcggtgtggc
     2401 tcggcccgcg gtcggcccgg gccggcgggg ccgccgcctt cctgctcgcc gccgcggacg
     2461 tggtcgcggt ctcgcggcag gcgcgcgtcg ggcccgccct ccccgccgag cttggccggg
     2521 gccgtgaccc ggccgccgag caggccctgt tc
//
LOCUS       DASPYY010000503         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_84197, whole genome shotgun sequence.
ACCESSION   DASPYY010000503 DASPYY010000000
VERSION     DASPYY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84197"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..278)
                     /locus_tag="VEP73_08010"
     CDS             complement(63..278)
                     /locus_tag="VEP73_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26406.1"
                     /translation="MPNGKEEPEEAGSYRCERCGVLRSAASGCTCGRDGAERIAPQGW
                     DPELTRSARPRLRIVLRPEQPKADPPA"
     gene            587..1363
                     /gene="uppS"
                     /locus_tag="VEP73_08015"
     CDS             587..1363
                     /gene="uppS"
                     /locus_tag="VEP73_08015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYT26407.1"
                     /translation="MGGSALLYRLYERRLAADLRRGGGTPRHVGVILDGNRRWARELG
                     MGSSEGHRRGAAQIDHLLAWAAELHIPVVTLWLLSTENLLGRHPDELAELLGIIEDKV
                     AQLARDTGPDGYRIVPVGQLDALPAATRARLLAAGEVSRAHTGGVLHVAVGYGGREEI
                     AEAVQRLLAEYAEKGVDLAEAAERVTPDEIARHLYTAGMPDPDLIIRTSGEVRLSGFL
                     LWQSAHAEYYFADTYWPDFRKVDFLRALRSYAQRQRRFGR"
     gene            1367..1843
                     /locus_tag="VEP73_08020"
     CDS             1367..1843
                     /locus_tag="VEP73_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26408.1"
                     /translation="MARKEREAARRRHAIDRARREAGWEPAPKPREPRPASRGQGRSR
                     PSTKPDGGRRPPAPLKKMEVGDIDRRGRVFERAMVLHPRVALRVYLAVFAVAVLSLFR
                     PELRSVSYFAFAVGMLVIADGQPKRRTALLFVAIAAFLLVAGVVLTVAQLTGRASG"
     gene            1836..>2481
                     /locus_tag="VEP73_08025"
     CDS             1836..>2481
                     /locus_tag="VEP73_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26409.1"
                     /translation="MADAGSFPRRYARTQGYTLGRPRGFTVAADGSRVAFLRSAGGDD
                     PVNRLYVLDVASGAERLVADPATLLGAAAESEARGSEARLGVPTGTGQGVPSGTGQGV
                     PTGTQRAVASGAAEDLPADERARRERSRERAGGIVAYAADDDLEMAAFALGGRLFVAD
                     LASGGGPSSGVRELPAAGPVFDPRPDPRGRRVAYVTDGALHVSEARRPEARLGVP"
BASE COUNT          290 a          944 c          924 g          323 t
ORIGIN      
        1 aggcccggca tgcagcaggg cgaggtgggt ggcggggtgc caggggccgc gctcggccgg
       61 cgctaggcgg gcgggtcggc cttcggctgc tcgggccgca gcacgatccg cagccgcggg
      121 cgggccgagc gggtcagctc cgggtcccaa ccctggggcg cgatccgctc ggccccgtcc
      181 cggccgcacg tgcaccccga cgctgccgac ctgagcacgc cgcagcgctc gcagcggtag
      241 gagccagcct cttccggttc ctctttcccg ttcggcacgg ctctcccttt cccgtccagc
      301 acgccgaggc gcgcctccga gccgccctcc acgtccgtcc atggcctccc tcccagggta
      361 gcggtccccg gcccacgagc gccggcaggg tctcgcctgg cggccgggcg gccgtgcacc
      421 atcgcgcgat ccgcgctgta gcatgacgcg cgggtcaccg ccgtgaccac gttacctctc
      481 ccgttcggca cgccgaggcg tgcctccgat gtccctttcc cgttcggcac gccgaggcgt
      541 gcctccgaat atcccagacc gcgcaggact cgacccggag ctgcccgtgg gcggaagcgc
      601 tctcctctac cggctctacg aacgccgctt ggccgccgac ctccgccgcg gggggggaac
      661 gccgcggcac gtcggtgtca ttctggacgg caaccggcgc tgggcccggg agctcggcat
      721 gggcagctcg gagggtcacc gccggggcgc cgcgcagatc gaccacctgc tcgcctgggc
      781 cgccgagctg cacatcccgg tcgtgaccct atggctgctc tcgaccgaga acctgctcgg
      841 gcgccacccc gacgagctcg ccgagctgct cggcatcatc gaggacaagg tcgcccagct
      901 cgcccgcgac accggcccgg acggctaccg catcgtgccg gtcggccagc tcgacgcgct
      961 gccggcggcg acccgggcgc ggctgctcgc ggccggggag gtgtcgcggg cgcacaccgg
     1021 cggcgtcctg cacgtcgccg tcggctatgg cgggcgcgag gagatcgccg aggcggtcca
     1081 gcggctgctc gccgagtacg ccgagaaggg cgtcgacctg gccgaggcgg ccgagcgggt
     1141 gacccccgac gagatcgccc gccacctgta caccgccggg atgcccgacc ccgacctcat
     1201 catccgaacg tccggggagg tgcgcctctc cgggttcctg ctgtggcagt ccgcccacgc
     1261 cgagtactac tttgccgaca cgtactggcc ggacttccgc aaggtggact tcctgcgggc
     1321 gttgcggtcg tacgcccagc ggcagcggcg cttcgggagg tagcacgtgg cacgaaagga
     1381 acgggaggcg gcacgccgcc ggcatgccat cgaccgcgcc cgccgggagg cgggctggga
     1441 gccggcgccc aagccgaggg agccgcggcc ggccagccgt gggcagggcc ggtcccggcc
     1501 ctccacgaag ccggacggcg gccggcgccc tccggcgccg ctgaagaaga tggaggtcgg
     1561 cgacatcgat cgccgcgggc gggtgttcga gcgcgcgatg gtgctccacc cgcgcgtcgc
     1621 cctgcgggtc tacctcgccg tgttcgccgt cgcggtgctc agcctgttcc ggcctgagct
     1681 gcgcagcgtg agctacttcg cgttcgcggt ggggatgctg gtgatcgccg acgggcagcc
     1741 caagcgcagg acggcgctcc tcttcgtcgc catcgccgca ttcctgctgg tggccggggt
     1801 cgtgctcacg gtcgcgcagc tcaccggccg cgccagtggc tgacgccggg tcgttccccc
     1861 gcaggtacgc gcgcacccag ggctacaccc tcgggcggcc gcgcggcttc accgtcgcgg
     1921 ccgacggctc ccgggtcgcc ttcctccgct ccgcgggcgg cgacgacccg gtcaaccgcc
     1981 tgtatgtcct cgacgtcgcc tccggtgcgg agcggctcgt ggccgacccg gctaccctgc
     2041 tgggcgccgc ggccgagtcg gaggcacgcg gttcggaggc acgccttggc gtgccgaccg
     2101 ggaccgggca aggcgtgccg agcgggacag ggcaaggcgt gccgaccggg acacagagag
     2161 ccgtggcgag cggggcagcc gaggacctgc ccgccgacga gcgggcgcgt cgcgagcgca
     2221 gcagggaacg ggccggcggc atcgtcgcct acgcggccga cgacgacctc gagatggccg
     2281 cgttcgccct tggcggccgc ttgttcgtgg ccgacctggc ctcgggcggc ggcccctcgt
     2341 cgggtgtgcg cgagctgccg gcggccgggc cggtgttcga cccgcggccg gacccgcgcg
     2401 ggcgccgcgt cgcctacgtg acggacggcg cactgcacgt gtcggaggca cgccgcccag
     2461 aggcgcgcct tggcgtgccg a
//
LOCUS       DASPYY010000504         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1491898, whole genome shotgun sequence.
ACCESSION   DASPYY010000504 DASPYY010000000
VERSION     DASPYY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491898"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VEP73_08030"
     CDS             complement(<1..710)
                     /locus_tag="VEP73_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYT26410.1"
                     /translation="MKDVGIVGLPLSGKTTLFNALTRAGVPAGGAAGKANLAAVSVPE
                     PRVDVLADLERSRKRVYVQVRFVDVAGLPAGPKGSGLSAQGLGALREVDALALVLAAF
                     EGSGDPVDAAADLELELTVADLSSIAGGAEKAAKKARAGVKEGALEAATLARARDLLD
                     AGTPLRQAPWEPEELKVLRNFSPLTLKPALAVVNLGEDQAATAATRAAEVEEALSVPT
                     VALPAALEAEAADLPPEDA"
     gene            927..2204
                     /locus_tag="VEP73_08035"
     CDS             927..2204
                     /locus_tag="VEP73_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26411.1"
                     /translation="MQPAAQRTSRGPGRVLLVDDHSAMREVLRSLLEEVGDWQVVGEA
                     ATGEAAVDLVAATAPDVVVMDCRMPGGGGVAATRAITDRFPAVAVVAHTAYADEAYVR
                     EMVAAGARGYVLKGDSPAAILEALAAARRGGARLSAEVAGPALDDLRALYQAALTRTE
                     ALHAENAELAAAVARLKAVDRAKDEFLALVGHELRTPVTVVMGMAETLAARPEVASGP
                     QGRELLDRLAAHGRRLAARVEQLLAASAYAAGRSPALVTEAVDVAELVIECADAHRAD
                     TPPLLVALDLPETPRPAAADPTGLRAVLEALLDNAAAAAPPGSQVRVTVEQPPGWTRI
                     AVADRGAGVAPADRDRIFDPFTQLDAGSTRRVGGIGLGLFLVERLVAGMGGRVEVADN
                     PGGGARFTVELPDPGQADDLEAPVPVPATRPAG"
BASE COUNT          272 a          871 c          879 g          266 t
ORIGIN      
        1 gcgtcctcgg gcggcaggtc ggccgcctcg gcctcgagcg ccgccggcag ggccaccgtc
       61 gggacgctga gcgcctcctc gacctcggcg gcccgcgtgg ccgccgttgc tgcctggtcc
      121 tcgccgaggt tcacgaccgc gagcgccggc ttgagcgtga ggggggagaa gttgcgcagg
      181 accttgagct cttccggctc ccagggcgcc tggcggagcg gcgtcccggc gtcgagcagg
      241 tcgcgcgcgc gggccagggt ggccgcctcc agggcgccct ccttcacccc ggcgcgggcc
      301 ttcttcgccg ccttctcggc gccgcccgcg atgctcgaga ggtcggcgac cgtgagctcg
      361 agctcgaggt cggccgctgc gtcgaccggg tcgccgctcc cctcgaacgc ggcgagcacc
      421 aacgcgaggg cgtccacctc gcgcagggcg ccgagcccct gcgccgacag cccggagccc
      481 ttggggccgg ccggcaaccc ggccacgtcc acgaagcgca cctggacgta cacccgcttg
      541 cgggagcgct cgaggtcggc gaggacgtcc acccgcggct cgggcacgga gacggcggcg
      601 aggttggcct tgccggccgc cccgcccgcg gggacgcccg cccgggtcag agcgttgaac
      661 agcgtcgtct tccccgacag gggcaggccg acgatgccaa catccttcat ctggacgagc
      721 tcccgggacg gacgcgccga atggttgaga ccggcgcggg aaagcgcgat tctacagccc
      781 gcacgacggc caccggcgtg agaccggcat ttcccccgac tgttccacgg aataacgccg
      841 gctggccgac agcaggcggg gcggacgggt cccgacatgg ccgccgaggc ggcggacgac
      901 cggcagcatc ggagatcgga gggggcgtgc agccagcagc gcagcgaacc tcgcggggcc
      961 ccggccgggt cctgctcgtc gacgaccact cggcgatgcg ggaggtgctg cgctcgctgc
     1021 tcgaggaggt cggggactgg caggtggtcg gcgaggcggc caccggcgag gccgcggtcg
     1081 acctggtcgc cgccaccgct cccgacgtgg tggtgatgga ctgcaggatg ccgggcggag
     1141 gcggggtggc cgcgacccgc gcgatcaccg accggttccc ggccgtggcg gtggtcgcgc
     1201 acaccgccta tgccgacgag gcgtacgtcc gcgagatggt ggccgccggg gcgcgggggt
     1261 acgtgctgaa gggggacagc ccggcggcga tcctcgaggc cctggccgcg gcccgccggg
     1321 gtggcgcccg cctctcggcc gaggtggccg gtccggccct cgacgacctg cgcgccctct
     1381 accaggccgc gctcacccgc acggaggcgc tccacgccga gaacgccgag ctggccgcgg
     1441 cggtcgcgcg cctgaaggcg gtcgaccggg ccaaggacga gttcctcgcc ctggtcggcc
     1501 acgagctgcg gacgccggtc acggtcgtaa tgggcatggc ggagacgctc gcggcccgtc
     1561 ccgaggtcgc ctccggtccg cagggacggg agctgctcga ccgtctggcc gcccacggcc
     1621 ggcgcctggc cgctcgcgtc gagcagctgc tggcggcgag cgcctacgcc gccggccgca
     1681 gcccagcgct cgtcaccgaa gccgtcgacg tcgccgagct ggtcatcgag tgcgccgacg
     1741 cccaccgcgc cgacacgccg ccgctcctgg tcgccctcga ccttcccgag acgccccggc
     1801 cggccgccgc cgatcccacc ggcctccgcg cggtgctgga ggcgctgctc gacaacgcgg
     1861 ccgcggccgc accgccgggc agccaggtcc gcgtcaccgt ggagcagcct ccggggtgga
     1921 cgcgcatcgc cgttgccgac cggggggccg gggtggcgcc ggccgaccgc gaccgcatct
     1981 tcgatccctt cacgcagctc gacgccggct cgacccggcg ggtgggcggg atcggcctgg
     2041 ggctgttcct ggtcgagcgc ctcgtggccg gtatgggcgg ccgggtcgag gtcgccgaca
     2101 acccgggcgg cggcgcccgc ttcaccgtcg agctgcccga cccaggacag gccgacgatc
     2161 tcgaggcgcc cgtcccggtc ccggccaccc gccccgccgg ctgagagcac gccgggcctg
     2221 gcgcgcttca ggcaggcggg gccagggact cgatgcgccc ggcgaggcgg aggtccttct
     2281 cggtgagg
//
LOCUS       DASPYY010000505         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1492191, whole genome shotgun sequence.
ACCESSION   DASPYY010000505 DASPYY010000000
VERSION     DASPYY010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492191"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VEP73_08040"
     CDS             <1..1048
                     /locus_tag="VEP73_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYT26412.1"
                     /translation="NDLDPALVEDVIMGCVMQVGEQALNVGRNAALAAGFPESTVGTT
                     VDRQCGSSQQAAHFAAQGVIAGAYDVVVAAGVESMTRVPMGASFTAGSQPFGPKMFAR
                     YQERGGLVPQGISAELIAEKWDISREDNDRFSLESHLRAARATDEGRFEQEIIPVPVT
                     GEEGAALFSRDEGIRPDSSLEKLASLKPAFRPDGQVTAGNSSQISDGAAALLIAGRDT
                     AEAMGLRPRARFVSFALAGVDPVTMLTGPIPATEKALAKAGLGLADVDVVEINEAFAS
                     VVLAWGREVDPDWERVNPNGGAIALGHPLGASGARLMTDLLAELERRGGRYGLQVMCE
                     GGGMANATVIERLG"
     gene            1108..1458
                     /locus_tag="VEP73_08045"
     CDS             1108..1458
                     /locus_tag="VEP73_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26413.1"
                     /translation="MTRRKRLLWLLALVVAALLVLTRRSDVLVAILIGALVLASGRAV
                     FRGYSVTKGPVGPPEDVAETGNLLVFACETCGEQLVLLRKGTDAPPRHCAEKMVLRLV
                     PTPDRQANLPDGLS"
     gene            complement(1455..1703)
                     /locus_tag="VEP73_08050"
     CDS             complement(1455..1703)
                     /locus_tag="VEP73_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYT26414.1"
                     /translation="MPVYEYACRACGAAEEHLQAMGSDAPGPCTACGGELRRRFSRVA
                     VKYQGWGFTSTDDLVPDDRPRRDFRELRERAERIADGG"
     gene            complement(1773..>2031)
                     /locus_tag="VEP73_08055"
     CDS             complement(1773..>2031)
                     /locus_tag="VEP73_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HYT26415.1"
                     /translation="RKDLAAMPKSRSKRRRYQPPPRPRPKPSAPWVGPTIVVCFAVGL
                     LYILLYYMDALPGPVRNLNQINLAIGFAPVIAGLLLATRWT"
BASE COUNT          289 a          731 c          729 g          282 t
ORIGIN      
        1 caacgacctc gatcccgccc tcgtcgaaga cgtgatcatg ggctgcgtca tgcaggtcgg
       61 cgagcaggcc ctgaacgtgg gccgcaacgc cgccctcgcc gccgggttcc cggaatcgac
      121 ggtcggcacg accgtcgacc gtcagtgcgg gtcgtcccag caggccgcgc acttcgcggc
      181 ccagggcgtc atcgcggggg cgtatgacgt cgtggtcgcc gccggcgtcg agagcatgac
      241 ccgggtgccg atgggcgcgt cgttcaccgc cggcagccag cccttcggtc ccaagatgtt
      301 cgcccgctac caggagcggg gcggtctcgt cccgcagggg atctcggccg agctcatcgc
      361 cgagaagtgg gacatctcgc gagaggacaa cgatcgcttc tctctcgagt cacacctgcg
      421 cgccgcgcgt gctaccgacg agggacgctt cgagcaggag atcatcccgg tgccggtcac
      481 gggcgaggag ggtgccgccc tcttctcgcg tgacgagggc atcaggcccg actcgtcgct
      541 cgagaagctc gccagcctga agccggcgtt caggccggac gggcaggtca cggcgggcaa
      601 ctcgagccag atctcggacg gcgccgccgc cctgctgatc gccgggcgcg acaccgccga
      661 ggccatgggc ctgcgccccc gggcccggtt cgtcagcttc gcgctcgccg gggtggaccc
      721 ggtcacgatg ctcaccggcc cgatccccgc cacggagaag gccctcgcca aggccggcct
      781 cggcctcgcc gacgtcgacg tggtggagat caacgaggcg ttcgcgagcg tcgtcctggc
      841 ctggggccgc gaggtcgacc ccgactggga gcgggtgaac cccaacggcg gcgcgatcgc
      901 cctcggccac ccgctcggcg cctccggcgc ccgcctcatg accgacctgc tcgccgagct
      961 cgagcggcgc ggcggccggt acgggctcca ggtcatgtgc gagggcggcg ggatggcgaa
     1021 cgccaccgtc atcgagcggc tcggatagcg cgccgggcgg ccgatggccg gctccctacg
     1081 ccgcctggca cgaggtagca tgagcgcatg acccgccgga agcgcctgct gtggctgctc
     1141 gcgctcgtcg tagccgccct gctcgtcctg accaggcgga gcgacgtgct cgtcgcgatc
     1201 ctgatcggcg cgctggtgct ggcgagcggc cgggccgtgt tccgcggcta ctcggtcacc
     1261 aaggggccgg tcggaccccc cgaggacgtc gccgagaccg gcaacctgct cgtgttcgcc
     1321 tgcgagacct gcggcgagca gctcgtcctg ctccgcaagg ggaccgacgc cccgccccgc
     1381 cactgcgccg agaagatggt gctgcgcctg gtcccgacgc ccgaccgcca ggcgaacctg
     1441 ccggacgggc tgagctagcc gccgtccgcg atgcgctcgg cacgctcgcg cagctcgcgg
     1501 aagtccctcc tcggccggtc gtcgggcacg aggtcgtcgg tgctggtgaa cccccacccc
     1561 tggtacttca ccgccacccg ggagaagcgc ctgcgcagct cgccgccgca ggccgtgcag
     1621 ggccccgggg cgtcgctgcc catcgcctgc aggtgctcct cggcggcgcc gcaggcacgg
     1681 caggcgtact cgtagactgg catcgctatc cacaaccttg tccccagctg gggacgaatc
     1741 acagggatgt agttcggcag ctcagcggca tctcaggtcc accgggtcgc cagaaggagg
     1801 ccggcgatca cgggtgcgaa cccgatggcg aggttgatct ggttcaggtt gcgcaccgga
     1861 ccgggaagcg cgtccatgta gtacaacagg atgtagagca gaccgacggc gaagcagacg
     1921 acgatcgtgg ggccgaccca cggagccgac ggcttgggtc tgggacgcgg cggcggctgg
     1981 taccggcgcc gcttggagcg tgacttgggc atcgcggcaa ggtcctttcg g
//
LOCUS       DASPYY010000506         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2474756, whole genome shotgun sequence.
ACCESSION   DASPYY010000506 DASPYY010000000
VERSION     DASPYY010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474756"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VEP73_08060"
     CDS             <1..533
                     /locus_tag="VEP73_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYT26416.1"
                     /translation="LVAFLVLCLLGYFAPNIVLLNKVQRRQAQIRRALPDSIDLLTIS
                     VEAGLGFDAALAHVSRNTTGPLAEEFYRTLQEVQLGRSRGEAMRNLADRSSVPELSTF
                     VLAMVQADVFGISVANVLRIQSREMRKKRRQMAEERAMKVPIKVLFPVLFCIFPALFV
                     VILGPAIMRIAAAFSR"
     gene            2223..>3352
                     /locus_tag="VEP73_08065"
     CDS             2223..>3352
                     /locus_tag="VEP73_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26417.1"
                     /translation="MSGAIPEAATAAVAEQATSGQPLGEPPSEEAEATTPEPTRAAVL
                     DVAATGEGEPPAQAQGAAVADVPDVTATAEPVAGEPVAGEPAAGEPAVEEAAAESQAT
                     AEEAAPTAGREDQAAVAGEPAVPVEEEVAAPHEPVPAPEEPAEAVARTGTAERPTATA
                     DVQETREEAAESAEESSAAADGPVAAGAVEAPEAGGAAEPPVAEAGEAARAGEAEEAP
                     ATEVEGAEPVDGVEARVAGAGAEVEAETETEADAEAESAMEGPEPAAAGPPSEEATEE
                     ATEEAAETEAARAGPGEAPGQTPPGRPAGAGLRGGGPAAPPAGPPMPPKPDPARAANG
                     DQPAADAGPAVAPKPAAETGPPAPAEPLAPVASAPPDAASSR"
BASE COUNT          388 a         1385 c         1271 g          308 t
ORIGIN      
        1 tgctggtcgc attcctcgtg ctctgcctgc ttggctactt cgcgccgaac atcgtcctgc
       61 tcaacaaggt ccagcgccgc caggcccaga tccggcgggc cctgcccgac tcgatcgacc
      121 tgctcaccat cagcgtcgag gccggcctcg gcttcgacgc cgccctcgcg cacgtctccc
      181 gcaacaccac cggcccgctc gcggaggagt tctaccgcac gctgcaggag gtgcagctcg
      241 gccggagccg cggcgaggcc atgcgcaacc tcgccgaccg ttcgagcgtc cccgagctct
      301 cgacgttcgt cctggcgatg gtgcaagctg acgtcttcgg catctcggtg gccaacgtcc
      361 tccgcatcca gtcgcgcgag atgcgcaaga agcggcgcca gatggccgag gaacgggcca
      421 tgaaggtgcc gatcaaggtc ctgttccccg tcctgttctg tatcttccca gccctcttcg
      481 tcgtcatcct cggaccggcc atcatgcgta ttgccgcagc cttctcccgg taggacgagc
      541 ccgtgtcccg ccatgtcgtc ctcctgtcac gcgacgcctc gctcgcggtg gcgctgcgca
      601 ccctgctcga ccaggccgac ctggtgacgg tgctcggctc cccggccgac tggtcgacga
      661 tcccgggcca gcccgtcgac accgtcgtcg tcgacgtgcc ctcggcgcgc cgcgaggccg
      721 tcatcggcga ggtccggtac cggttctccg gccctctcgt cgtgctgctc gcacagcggg
      781 acgacccgaa ggcgctgctg gcggacccga gcctgtcggt gatgaagcgg ccgttcgcga
      841 tgatggagtt ctggggcgtc ctgaccggca gggtggcgtc ccccggcccc gtctccgccc
      901 cgccctcgtc ccgccgacgg tccagagtgt gggccgagcg gccccagccg ccggcccccg
      961 gcccttccgc accgcagccg tcgcagccac ccggaggcgc ccgcgcggcc gagccggaca
     1021 ggggcgccac gcaggcgccg ggtggcgggg ccaccccgcc gccgggcgtg gaggccacgc
     1081 cgccgcccgg cgcgaggccg gcgcccccgc ccggtgcgcg gcccgtgccg cctcccggtg
     1141 cgcggcccgc gccggaggag cccgcgtcgc cccgcgccag cggtcgtccc ggggtgccgg
     1201 tcccgcccgc cccgggtccg cccaccccgg gtccacccac cccgggtcca cccatcccgg
     1261 gtccgcccgc cggtcgcccg acggcaacgc cgacagccgc gccgaccccg gaccggggcc
     1321 ccgagccgcc agggccgtcc gagcgcgggg ccggagccgg ccagggggaa gccgccctcc
     1381 ccggcccggc agctcgcacc cgcggccgag ctccggacgc ggggccggtg ccgtcgggct
     1441 ccggggcgac acgggcgacc ccgggtccca gcggcccgaa gggagacgcc gggccgcccg
     1501 ccccaccccc ggtgccgccc ggcggtggcg gccccgcggc gccgccgaag ggccgccgcc
     1561 tcctgcgcgg gttcggccgc cggctcggtg ggggccgcga gccggccgcg cccgccagcc
     1621 ccgcccctgc gccggagccg gccgccccct cgcgcgaggc gtccttcacc tcctggttcg
     1681 acgcgggcga ccggccccgg ccggggccgc cgccccctgg ccctgggccg gcggtgggcc
     1741 gccctggccg gacgggccgg gagccaggca agccgggtgg gcagcctcct cgccctggaa
     1801 cggcggcgcc cggctccagc gtcggctcgg cggccgcccc ggaggccgcc gagccgacct
     1861 ccccggcacc cgccggctcg gcgcctgaac gaccgggtga acggcccccg acggcccgca
     1921 ccctccggta ccgccccgga gtccctcggc ccgctcctcc ttcaccgcct ccgactccgg
     1981 tcgccacacc gcgcgacccg tcgccgacgc cgcgggcgcc ggaggaagcg ccgggccgga
     2041 ccgagccgat cgccccggcg gaggcccctc ccgagcggga caggggaccg gtgccgcaag
     2101 caccgccctc cggcccaccg gcagcgaccc cgccgtctgg agccgtcggc gcgccggagg
     2161 gcgcggccgg cgcaccggag ggtccccccg gcgaagccgc ccccgacgcc ccgaaggagc
     2221 cggtgtccgg tgcgatcccg gaggcggcca ccgctgccgt ggccgagcag gcgacctccg
     2281 ggcagccgct cggcgagccg ccgtccgagg aggccgaagc caccacaccg gagcccaccc
     2341 gggccgctgt gctggatgtg gccgcgaccg gcgagggcga gccgcccgcg caggcccagg
     2401 gagccgccgt cgcggacgtg cccgacgtga cagccaccgc ggagccggtc gccggggagc
     2461 cggtcgccgg ggagccggcc gccggggagc cggccgtcga ggaggccgcc gcggagtccc
     2521 aagcgaccgc ggaggaagcg gctccaaccg caggacggga agatcaggcc gcggtcgcgg
     2581 gcgagccggc cgtcccggtc gaggaggagg tcgccgctcc gcacgagccg gttccagcgc
     2641 cagaggaacc ggccgaggcg gttgcgcgca cgggcacggc ggagcggccc accgcgaccg
     2701 cagacgtcca ggagacccgc gaggaggccg ccgaatcggc ggaggagtcc agtgccgccg
     2761 ccgacgggcc ggtcgccgcc ggggccgtgg aggcccccga ggccgggggg gccgccgagc
     2821 caccggtcgc cgaggccggg gaggctgcca gggccgggga ggccgaagag gcaccggcca
     2881 ccgaggtgga aggcgctgag ccggtcgacg gcgtggaggc gcgggtcgcc ggggccgggg
     2941 cagaggtcga ggcggagacc gagaccgagg cggacgccga ggcggagtcg gccatggagg
     3001 gtcccgagcc cgccgctgcg ggaccgcctt ccgaggaggc gaccgaggag gcgaccgagg
     3061 aggctgccga gacagaggcc gctcgcgccg ggcccggcga ggcgccggga cagacgccgc
     3121 ccgggagacc ggccggcgcc gggctgcgcg gcggcggtcc ggccgcgcca cccgccgggc
     3181 cacccatgcc cccgaagccg gacccggcga gagcggcgaa cggcgaccag ccggccgcgg
     3241 acgcgggacc ggcggtcgcg ccgaagcccg ccgccgagac gggaccgccg gctccggccg
     3301 agccgctcgc cccggtggcc tccgcgcccc cggacgcggc gtcatcgcgg aa
//
LOCUS       DASPYY010000507         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1914099, whole genome shotgun sequence.
ACCESSION   DASPYY010000507 DASPYY010000000
VERSION     DASPYY010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914099"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEP73_08070"
     CDS             <1..183
                     /locus_tag="VEP73_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26418.1"
                     /translation="LLDLPEPTKFPAVLELVLELRERSRAARDFAAADLVRDRLAAVG
                     IRVEDRAGGPRWHLAR"
     gene            165..1220
                     /gene="rlmB"
                     /locus_tag="VEP73_08075"
     CDS             165..1220
                     /gene="rlmB"
                     /locus_tag="VEP73_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HYT26419.1"
                     /translation="MAPRQVGGPGPGSGKPRRRQRPERPSARGAPGGRNPRGGDGRGG
                     DPRGGDAEVRRPRAPAEKRRAAPAGGGRSAKQGRRREQLQAARAAVQPSPGRELIEGR
                     RPAIEALRSGRAVDRVLVAAGSAASGTLAELVALARDRGVPVETVPRSVIDAEARTGA
                     HQGVLAVVAPLEPIGLDELLAVPLGSAEPPFFLALDGVEDPHNLGALARSAEAAGCHG
                     MIVPKHRSAPLSAVAVKSSAGALGHLPVAEATNLARALDRLRDSGIWCVGLDGGADAS
                     LFDLDLAAEPVCVVVGGEGDGLHRLVREACDMVVRIPMAGHVESLNASVAGALALFEI
                     RRRRTPGPQREPVPGPV"
     gene            1271..1343
                     /locus_tag="VEP73_08080"
     tRNA            1271..1343
                     /locus_tag="VEP73_08080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Thr,seq:tgt)
BASE COUNT          170 a          622 c          588 g          201 t
ORIGIN      
        1 ctgctcgacc tgcccgagcc gacgaagttc cccgccgtgc tcgagctcgt gctcgagctg
       61 cgcgagcggt cccgggcggc gcgcgacttc gccgccgccg acctcgtccg cgaccggctc
      121 gccgccgtcg gcatccgggt cgaggaccgg gccggaggcc cccgttggca cctcgccagg
      181 taggcgggcc ggggccggga tcgggcaagc cgcggcgccg ccagcgtccg gagcgcccgt
      241 cagcccgcgg cgcccctggg ggcaggaacc cccgaggcgg ggacggccgt ggcggggacc
      301 cacgtggcgg ggacgccgag gtccggcgcc cccgggcacc ggccgagaag cggcgcgcgg
      361 cccccgccgg cgggggccgc tccgccaagc agggccgccg ccgcgagcag ctccaggctg
      421 ccagggccgc cgtccagccc agccctggca gggagctgat cgagggccgc cgcccggcga
      481 tcgaggcgct gcgatccggc cgcgccgtcg accgggtcct ggtggcggcc ggcagcgccg
      541 ccagcggcac cctcgccgag ctggtcgcgc tcgcccgcga ccgcggcgtg ccggtcgaga
      601 cggtcccccg ctcggtcatc gacgcggagg cgcggaccgg cgctcaccag ggcgtgctcg
      661 ccgtcgtggc gccgctcgag ccgatcggcc tcgacgagct gctcgcggtg ccgctcggca
      721 gcgcggagcc gccgttcttc ctcgccctcg acggggtcga ggaccctcac aacctgggcg
      781 cgctggcccg cagcgccgaa gcggccggct gccacggcat gatcgtgccg aagcaccgca
      841 gcgcgccgct gtccgccgtc gccgtgaagt cctcggccgg cgcgctcggg cacctgccgg
      901 tcgcggaggc caccaacctc gcccgtgccc tcgaccggct gcgcgactcg ggcatctggt
      961 gcgtcggcct ggacggcggg gcggacgcga gcctgttcga cctcgacctt gccgccgagc
     1021 ccgtctgtgt cgtggtcggc ggcgagggcg acggcctgca ccgcctcgtc cgtgaggcgt
     1081 gcgacatggt cgtgcgcatc ccgatggccg gccacgtcga gagcctgaac gcctccgtcg
     1141 ccggcgcgct cgcgctgttc gagatccgcc gccgccgcac cccgggcccg cagcgggagc
     1201 cggtccccgg gccggtctga cctcgggcct ggcccatccc gggcacaccc gggtacgctt
     1261 ccctcacccg gctggcgtgg cgcaaccggc agcgcagctg tcttgtaaac agcaggttgg
     1321 gggttcgagt ccccccgcca gctcccaaac ccgcaggtca gcgatctgca caccttgctt
     1381 tgcttggttt gcgtcattcc gcggtcattc ttcaaccccc ggtcgcgctc tggcgccgct
     1441 cgtgatcggg gtgtcgccgg tcgtgttcgc gaccgtgctg cgtcgccgcg tccacccggg
     1501 cgggtgacca tgatcgcgat agcgtgcggc cgctcaagac ccggaggggt cgaggggaaa
     1561 cgtcccacgg ctggcaggcg t
//
LOCUS       DASPYY010000508         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1631644, whole genome shotgun sequence.
ACCESSION   DASPYY010000508 DASPYY010000000
VERSION     DASPYY010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631644"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VEP73_08085"
     CDS             <1..706
                     /locus_tag="VEP73_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HYT26420.1"
                     /translation="LAWVVNLGCIDLHPWPVRAFDTDHPDELRVDLDPTPEASFADVR
                     QVALVVREVLDEHGLVGFPKTSGSRGIHVHCRIRPEWEYLETRRAALALARAVERRVP
                     RLATSAWWKEERRGVFVDYNQNARDRTVASVYSVRPRPDARVSCALTWDEVTDVEPEE
                     LRMDTVPERVRTRGDPGAGIDRAAGGLEALLELAARDEREGLGDAPWPPHFPKATGEG
                     MRVAPSRARKRPPPQA"
     gene            781..1854
                     /locus_tag="VEP73_08090"
     CDS             781..1854
                     /locus_tag="VEP73_08090"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYT26421.1"
                     /translation="MDLPVMPPVKPMLAKSVSILPDGMLYEPKWDGFRCIAFRDGDEV
                     ELGSRNERPFTRYFPEVVAAVRAALPPRCVVDGEIVVVQDAGLDFEALQQRIHPAASR
                     VELLAGSTPASLIAFDLLALDDRSFMDRPFAERREALASVLAGVDAPVHLTPATDSSK
                     VAQRWFRVFEGAGLDGVVAKPLDLPYEQDQRVMFKVKHVRTADCVVAGFRWHKSGPVV
                     GSLLLGLYDDGELQHVGVSASFPMERRRELLDELAPYRLKVGDPHPWLGSVTSERIPG
                     GEPSRWNAKKDLSWEPLAPSLVVEVGYDHMEGRRFRHTAQFKRWRPDREPRSCTYAQL
                     ERPVAFDLAGILAQGGTPVLPDP"
     gene            1988..2206
                     /locus_tag="VEP73_08095"
     CDS             1988..2206
                     /locus_tag="VEP73_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26422.1"
                     /translation="MVHPAGAIRGRPEEVDASLRAIADDDGRDGADREVAASFLPFLG
                     ADDPGPGIARRRHGRGFSYTTPAFDLSA"
     gene            2326..>2757
                     /locus_tag="VEP73_08100"
     CDS             2326..>2757
                     /locus_tag="VEP73_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYT26423.1"
                     /translation="MSEYKLAAEPRSDTGKGAARRARAAGRVPAILYGHGMTPEPLTV
                     DSREFGHALRTDAGTNVLIELSVGGNGRLALAKEIQRHAVKGTFVHVDFLLVQRGEKV
                     QVQVPVHLVGDAPGVREGGIADQDLYQVNVEAEVTAVPEQVE"
BASE COUNT          349 a         1059 c          998 g          351 t
ORIGIN      
        1 cctggcctgg gtggtgaacc tcggctgcat cgacctgcac ccctggccgg tgcgggcctt
       61 cgacaccgac caccccgacg agctccgcgt cgacctggac ccgaccccgg aggcgtcctt
      121 cgccgacgtc cgccaggtcg ccctggtcgt gcgcgaggtg ctcgacgagc acggcctggt
      181 cgggttcccg aagacctcgg gctcacgcgg catccacgtg cactgccgca tccgtcccga
      241 gtgggagtat ctcgagacac ggcgggcggc gctcgccctc gcccgggcgg tcgagcgccg
      301 cgtcccgcgc ctggccacct cggcctggtg gaaggaggag cgccgcggcg tcttcgtcga
      361 ctacaaccag aacgcccgcg accggacggt ggcctcggtc tactcggtcc ggccccggcc
      421 agacgcccgg gtctcctgtg cgctgacctg ggacgaggtg accgacgtcg agccggagga
      481 gctccggatg gacaccgtgc ccgagcgggt tcggaccagg ggggacccgg gtgccgggat
      541 cgaccgggcc gcgggcggcc tggaggcgct gctcgagctg gcggcgcgcg acgagcgcga
      601 ggggctcggc gacgcgccct ggccgcccca cttcccgaag gccacggggg aggggatgcg
      661 ggtcgccccc agccgggccc gcaagcgccc gccgccccag gcctgagccc cggatccgcc
      721 gccccaggcc tgagcctcgg ggtccccacc cggcgcgccc cggcagggca gaatagcggc
      781 atggacctgc ccgtcatgcc cccggtcaag ccgatgctgg ccaagtcggt ctcgatcctg
      841 cccgacggga tgctctacga gcccaagtgg gacgggttcc gctgcatcgc gttccgggac
      901 ggcgacgagg tggagctcgg cagccgcaac gagcggccgt tcacccgcta cttccccgag
      961 gtcgtcgcgg cggtgcgcgc ggcgctcccg ccacggtgcg tcgtcgacgg cgagatcgtc
     1021 gtcgtccagg acgccggcct cgacttcgag gcgctgcagc agcgcatcca cccggccgcc
     1081 tcgcgcgtcg agctgctcgc cggcagcacg ccggcgtcgc tgatcgcgtt cgacctgctc
     1141 gccctcgacg accggtcgtt catggaccgt cccttcgccg agcggcggga ggcgctcgcg
     1201 tccgtgctcg ccggggtgga cgcgccggtg cacctgacgc ccgccaccga ctcctccaag
     1261 gtcgcccagc gctggttccg cgtattcgag ggcgcgggcc tcgacggcgt cgtcgccaag
     1321 ccgctcgacc tgccctacga gcaggaccag cgggtcatgt tcaaggtcaa gcacgtgcgc
     1381 acggcggact gcgtggtcgc cggcttccgc tggcacaaga gcggcccggt ggtcggctcg
     1441 ctgctgctcg gcctctacga cgacggcgag ctccagcacg tcggcgtctc ggcgtcgttc
     1501 ccgatggagc ggcggcggga gctgctcgac gagctcgcgc cctaccgcct gaaggtcggc
     1561 gacccccacc cgtggctcgg cagcgtgacc tccgagcgca tcccgggcgg cgagccctcg
     1621 cgctggaacg cgaagaagga cctgtcctgg gagccgctcg cgccgtcgct ggtcgtcgag
     1681 gtcggctacg accacatgga gggcaggcgc ttccggcaca ccgcgcagtt caagcgctgg
     1741 cggccggacc gggaacctcg gtcgtgcacc tacgcgcagc tcgagcggcc ggtcgccttc
     1801 gacctggccg ggatcctggc ccagggcggc acgcccgtcc tccccgaccc ctgagcggcc
     1861 cggggcggtc cggtgggagg gcgaccggcc ggtcgcggtc ggcggccagg agctcggcca
     1921 gcccaccagc atcgcccaga ccaggggcgg cttctgggca ctgcaccagg tcggcgacga
     1981 cgtcgtcgtg gtccacccgg cgggcgcgat ccgcgggcgg cccgaggagg tggacgccag
     2041 cctgcgggcg atcgccgacg acgacgggcg ggacggcgcc gaccgcgagg tggcagcctc
     2101 cttcctgccc ttcctgggcg ccgacgaccc cggccccggg atcgcgcgca ggcgccacgg
     2161 ccggggcttc tcctacacga ccccggcctt cgacctgagc gcctgaagcg tgctaccctc
     2221 gcctccgccc cgccggagcc ggggcttttg ccgcttgccg cgccgcggcg cggccgctcc
     2281 gcaccaggat ctgcgctcgc ccgacctcga ggaggccacc ccccaatgtc cgagtacaag
     2341 cttgccgccg agccccggtc ggacaccggg aagggagccg cccgccgcgc ccgtgccgcc
     2401 ggccgcgtgc ccgcgatcct ctacgggcac gggatgaccc cggagcccct gaccgtcgac
     2461 tccagggagt tcggccacgc cctgcgcacc gacgcgggga ccaacgtgct gatcgagctg
     2521 tcggtgggtg ggaacgggcg cctcgccctg gccaaggaga tccagcgcca cgccgtcaag
     2581 gggaccttcg tccacgtcga cttcctgctc gtccagcggg gcgagaaggt ccaggtccag
     2641 gtgcccgtgc acctcgtcgg cgacgcgccg ggcgtgcgcg agggcgggat cgccgaccag
     2701 gacctctacc aggtcaacgt cgaggccgag gtgaccgccg tgcccgagca ggtcgag
//
LOCUS       DASPYY010000509         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1914245, whole genome shotgun sequence.
ACCESSION   DASPYY010000509 DASPYY010000000
VERSION     DASPYY010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914245"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VEP73_08105"
     CDS             complement(<1..939)
                     /locus_tag="VEP73_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26424.1"
                     /translation="MTDGATPARPSRPPGRDGGPAARRRRLGLLGAVAVAGTGLVLAL
                     ATPAQAARGGPRALGARGPVAAGARGQPGSAAPGRAVAAPPGRAVAAPPGVLRVPVTG
                     VIDPFLAGAVRHAVQRAGREGDGLVVLELDTPGGLDSSMREIVQAILDAPVPVVGYVT
                     PRGAQAASAGTFIIAATSWAAMAPDTEIGAAHPVGISGQVLAEKVTNDAAAYVKGLAQ
                     NHRQSGPAVAAWYEDAVRHSVSLTATEAAAQGVVDDVYVNEAALLDALDGKTLKFGAG
                     RSATVHTLGAPVRTAGLGPAMLLLHRLVDPNIAFLLF"
     gene            complement(932..1513)
                     /locus_tag="VEP73_08110"
     CDS             complement(932..1513)
                     /locus_tag="VEP73_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYT26425.1"
                     /translation="MAPSSAQPPDPPEASSGADRPAVRVERSARRRKTVSAYREGDTV
                     VVLLPARMSRREEAHWVAAMLERLDARERRLQPPGDEELGRRARMLARRYLDLALEPR
                     SVRWAANMRTRYGSCTPEDRTIRLSDRLAAYPAWVRDYVLVHELAHLAVPNHSPAFWR
                     IVSRYPLAERARGFLIAKGLNDDGPLEEGEPDD"
BASE COUNT          309 a          827 c          896 g          294 t
ORIGIN      
        1 gaacagcagg aaggcgatgt tggggtcgac cagccggtgg agcagtagca tggccgggcc
       61 gagaccggcc gtgcgcaccg gggcgccgag cgtgtggacc gtggccgacc ggccggcccc
      121 gaacttgagg gtcttgccgt cgagagcgtc gagcagcgcg gcctcgttga cgtagacgtc
      181 gtcgacgacc ccctgcgccg cggcctccgt ggcggtgagg gagaccgagt ggcgcaccgc
      241 gtcctcgtac caggccgcga cggccggccc cgactgccgg tggttctggg ccaggccctt
      301 gacgtacgcg gcggcgtcgt tggtgacctt ctcggccagc acctggccgg agataccgac
      361 cgggtgcgct gccccgatct ccgtgtcggg cgccatcgcc gcccaggacg tggcggcgat
      421 gatgaaggtg cccgcgctgg cggcctgcgc ccccctcggg gtcacgtagc cgaccacggg
      481 aaccggcgcg tcgaggatgg cctggacgat ctcgcgcatg gagctgtcga gcccgcctgg
      541 cgtgtccagc tcgagcacga ccagcccgtc gccctcgcgc ccggcccgtt ggacggcgtg
      601 ccgcaccgcg cctgccagga aggggtcgat gaccccggtg accggcaccc gcagcacgcc
      661 gggcggggcg gcgacggcgc ggccgggcgg ggcggcgacg gcgcggccgg gcgccgcgct
      721 ccccggctgg ccccgcgcgc cagcggcaac cgggcctcgc gcgccgaggg ccctggggcc
      781 gcctcgggcg gcctgggccg gtgtggcgag cgcgaggacc agcccggtcc cggcgacggc
      841 gaccgcgccg aggagcccca ggcgccgccg tcgggcggca gggcccccgt cgcgaccggg
      901 cggtcgcgac ggtcgtgcag gggtcgcgcc gtcagtcatc gggttcccct tcctcgagtg
      961 gcccgtcgtc gttcagcccc ttggcgatca ggaagccgcg ggcgcgctcg gcgagcgggt
     1021 agcgggaaac gatccgccag aacgccggcg agtggttcgg caccgccagg tgggcgagct
     1081 cgtgaaccag cacgtagtcg cgtacccagg cggggtaggc ggccagccgg tcggacaggc
     1141 ggatggtgcg gtcctccggc gtgcacgacc cgtacctggt gcgcatgttg gctgcccagc
     1201 gcaccgagcg cggctcgagc gccaggtcga gatagcgtcg cgcgagcatc ctggcccggc
     1261 ggccgagctc ctcgtcgcct ggtggctgga gccggcgctc gcgggcgtcg agacgctcga
     1321 gcatggccgc cacccagtgc gcctcctcac ggcggctcat gcgggcgggc agcagcacga
     1381 ccacggtgtc gccctcgcgg taggcgctta ccgtcttgcg gcggcgggcg ctgcgctcga
     1441 cccggacggc cgggcggtcg gcgccggagg acgcctccgg cgggtcgggc ggttgggcgg
     1501 acgaaggagc catgcgcacc tcgggtcgaa ggcaccggga ccggccggcg gcccgcccgg
     1561 cactccgagt atgccactcg cccgtgacgc cgaccgaccc gcatcggtca cccaactttt
     1621 ttcgtccaca gcccggtgga tctgaacggc gcaggtcagg gacgcgatca acgctgctgt
     1681 cccgacgctc gtccccagct tgctctcctg ttgtccacag gctgcgccgc gccgtcaaca
     1741 accgggaggg gttctccaca ctcttgtcca caacgtcggg gacggtcgcg ttgacgcgcc
     1801 ccggcgggca tgccatcgtg cttcccgcac gaggcctccg ggaacggcga aggttctcgc
     1861 tctgtaaggg gaaggcagag cgcggacccg agccaaccgg aggtttcgtg tgcttgagct
     1921 gggaaaacgc tgggccagct cctggcggcg gccccgaaat catcacgcgg gcagcgtgcg
     1981 ggcagcaaac ggaccaaggg cgacttcggc aggcctgatt gcccgactag gtgtgcacct
     2041 ccccgctgac ccgccgcgca tccttcagcg gtgccctcca gaggtgttac ggctccgcac
     2101 ttacaagtga ggaggccgag ctggctcctg gcgacccgga ccaacgggag ggtgcatgga
     2161 ggacggtggg gatgaggcgc ttgcccgggc caaggaggcg gtcgcacacg gcgacgcgtg
     2221 gaccgcctcg gcaagggcgg ccgggtcgtc ggggaccacc gggtagacgc cggcgaccgc
     2281 cccgagctcg gtgagcagcg cggccagggg cgccgagagc gcgtcg
//
LOCUS       DASPYY010000510         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_649218, whole genome shotgun sequence.
ACCESSION   DASPYY010000510 DASPYY010000000
VERSION     DASPYY010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649218"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VEP73_08115"
     CDS             complement(<1..930)
                     /locus_tag="VEP73_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HYT26426.1"
                     /translation="MDDGFVPGLRLAGEFYAGAVRPRLGRAFPGLRYAAALIGAGSEV
                     LGFDTERSTDHDWGPRLLLFLDDEDAGRLGEDVDEALAGRLPDSFGGYPVRFGVTRDP
                     GSGARHRVEVCGLGGWLTWRLGFDPRDGVTASDWLGVPWQRLAEVTGGAVFEDRTGEL
                     TRVRTALSWYPRDVWCYVLGCQWSRIAAEEAFPGRCAEAGDELGSAVVTARLARDLMG
                     LWLLMNRRYPPYSKWLGRAFARAAGSAELGAELGAALAGPDWPSRERHLSRAYMIAAE
                     AHNRLGLTSPVDPATRPYYERPYQVLDAGRFAAA"
     gene            complement(948..1427)
                     /locus_tag="VEP73_08120"
     CDS             complement(948..1427)
                     /locus_tag="VEP73_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYT26427.1"
                     /translation="MTDIDPVEVTVHVPAAPADVFPYFTDPARYVQWMGSEARLEPVP
                     GGVYRVVMPDGFAAAGVFRRVERPRLVVFTWGFADDEAASRTKGGEAASVGAMPAGST
                     RVTVTLEDADGGTRVTLRHENLPSAELRAGHEVAWDTYLPRLAVVAAGGDPGADPHA"
     gene            1489..>1893
                     /locus_tag="VEP73_08125"
     CDS             1489..>1893
                     /locus_tag="VEP73_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26428.1"
                     /translation="MGAVTADLIDLAVAGDEEAFRQLVAPFERELKVHCYRMLGSVQD
                     AEDALQDALLAAWQGLAGFEGRASVRTWLYRVATSRCLNVRRSARRRQGTMPPGLTGA
                     TPPEPTRLGEVTWLEPYPDTLLEGLAAAEPGPE"
BASE COUNT          252 a          759 c          662 g          220 t
ORIGIN      
        1 cgcggcggcg aagcgcccgg cgtccaggac ctggtagggc cgctcgtagt agggccgggt
       61 ggccggatcg accggcgagg tgagccccag ccggttgtgc gcctccgcgg cgatcatgta
      121 cgcccggctc aggtgccgct cccgggacgg ccagtccggg ccggccagcg ccgcacccag
      181 ttcggccccc agttccgcgc tcccggccgc ccgcgcgaac gcgcggccca gccacttgct
      241 gtacggcggg taccggcggt tcatcagcag ccacaggccc atcaggtcgc gcgccagccg
      301 cgccgtgacc acggccgagc cgagctcgtc gccggcctcg gcgcaccggc cggggaacgc
      361 ctcctccgcc gcgatccggc tccactggca gcccagcacg tagcaccaca cgtcccgcgg
      421 gtaccagctc agcgcggtcc ggacgcgggt cagctcaccc gttcggtcct cgaaaaccgc
      481 cccgccggtg acctcggcca gcctctgcca cgggaccccc agccagtccg acgcggtcac
      541 gccgtcccgg gggtcgaagc cgagccgcca ggtcagccag ccacccaggc cgcaaacctc
      601 gacccggtgc cgcgccccgc tgcccgggtc gcgggtgacg ccgaacctca ccggatatcc
      661 gccgaacgag tccggcaacc gccccgccag cgcctcgtcg acgtcctcgc cgagccgccc
      721 ggcatcctcg tcgtcgagga acagcagcag cctcggtccc cagtcgtggt cggtggaccg
      781 ttcggtgtcg aagccgagca cctccgagcc ggccccgatc agcgcggccg cgtaccgcag
      841 cccgggaaac gcccggccga gccgcggacg gaccgccccg gcgtagaact cgcccgccag
      901 ccgcagcccg ggcacgaacc cgtcatccac cccgccatgg tgccccctca ggcatgcggg
      961 tcggcgcccg ggtcgccccc cgcggcgacg acggcgagcc gcggcaggta ggtatcccag
     1021 gcgacctcgt ggcccgcgcg caactcggcg ctgggcaggt tctcgtgccg gagcgtgacc
     1081 cgggtcccgc cgtcggcgtc ctcgagcgtc accgtgaccc gggtgctccc ggccggcatg
     1141 gcgccgacgc tggccgcctc gccccccttg gtccgcgacg ccgcctcgtc gtcggcgaat
     1201 ccccaggtga agaccaccag gcgcggccgc tcgacgcggc ggaacacccc agccgccgcg
     1261 aacccgtccg gcatgacgac ccggtacacg ccgcccggca ccggctccag cctggcctcg
     1321 ctgcccatcc attgcacata acgggctggg tccgtgaagt agggaaagac gtccgcgggc
     1381 gcggccggga cgtggacggt cacctcgacc gggtcaatgt cagtcatgac gggatagaca
     1441 ctaaggcgcc cggtaactgg gcgctggcgg cgtcgcggat atgcgacgat gggcgccgtg
     1501 accgcggacc tgatcgatct cgccgtcgcc ggtgacgagg aggcgttccg ccagctcgtc
     1561 gcccccttcg agcgggagct gaaggtgcac tgctaccgga tgctcggttc ggtgcaggac
     1621 gccgaggacg cgctgcagga cgcgctgctc gcggcctggc agggcctggc cgggttcgag
     1681 gggcgcgcct cggtccggac ctggctgtac cgggtggcca cctcccgctg cctgaacgtg
     1741 cgccggtcgg cgcgccgccg ccaggggacg atgccgccgg ggctcaccgg ggctacgccg
     1801 cccgagccga cccggctcgg cgaggtgacc tggctcgagc cgtacccgga caccctgctc
     1861 gagggcctgg ccgccgccga gcccgggccc gag
//
LOCUS       DASPYY010000511         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1351021, whole genome shotgun sequence.
ACCESSION   DASPYY010000511 DASPYY010000000
VERSION     DASPYY010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351021"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1897
                     /locus_tag="VEP73_08130"
     CDS             <1..>1897
                     /locus_tag="VEP73_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYT26429.1"
                     /translation="DQGLVRQNYLGGIFGGITRRQYEDVKRIPDVEVAAPIANLGYVA
                     PIQKLPVTIDRKVLQLDRDQVQLYRLRYRWLANHGLSRYEGGSVYAYYTRAHELVAVD
                     GFPAEVLPDGTRAQVCNPEHRMPAGSYLACFSERSPKVARLAGLPAGAVGGRADLHAP
                     ILLAAVDPLQEARLVGLDRAVVDGRFLRDSDRVTAAPPGTGGMRVRTIPVVASSRLYV
                     DQVLETSVERLHLPAGVDVARTLASPGAGRFVAGLRGQVVQRRHTSPLPLYERTLERW
                     NGPVTDLDLLVNAYWTVSPVAYRPLPGGRVAPVPTTNPDDVWRLRFSGTVRLPGSDDV
                     QYRAVHLHDGSTDVDQAGVVRAPAVRVVGRFDPERLPGFNPLSQVPLETYYPPEAEGA
                     DAASRKALGGRPLRPTTNIGGYLAQPPLLLTTIQALDAMTDPATYPTADHAAPISVIR
                     IRVAGVTGPDRVSRERIRRVAAAVVARTGLAVDVTAGSSPHSLLVDLPAGRDGQPPLL
                     VSEGWVKKGAAIVILAALDRKSVALFFLVLLTCACFVANGAFAAVRARRTEIGTLRCL
                     GWSAGRVFAVVLGEVAAIGLAAGILGTALAAAGVRVLHLHLAPSQMLLVAPVAVWLAV
                     LAGVPPAW"
BASE COUNT          213 a          749 c          670 g          265 t
ORIGIN      
        1 cgaccagggc ctcgtccgcc agaactacct cggcggcatc ttcgggggga tcacccggcg
       61 gcagtacgag gacgtcaagc ggatcccgga cgtggaggtg gccgccccga tcgccaacct
      121 cggctacgtc gcgcccatcc agaagctgcc ggtgacgatc gaccggaagg tgctgcagct
      181 ggaccgggac caggtccagc tgtaccgcct gcggtaccgc tggctggcca accacgggct
      241 gtcgcgctac gagggcggct cggtctacgc ctactacacg cgcgcgcacg agctggtcgc
      301 cgtggacggc ttcccggcgg aggtgctccc cgacggcacg cgggcccagg tctgcaaccc
      361 cgagcaccgc atgccggcgg gcagctacct cgcctgcttc tccgagcggt ccccgaaggt
      421 ggccaggctc gccgggctcc cggccggcgc ggtcggcggc cgggcggacc tccatgctcc
      481 gatcctgctc gccgcggtcg accctttgca ggaggccagg ctggtgggcc tggaccgggc
      541 cgtggtcgac ggccgcttcc tgcgcgactc cgaccgcgtc accgcggcgc ccccgggcac
      601 gggcgggatg cgggtgcgca cgatcccggt tgtcgcgagc tcccgcctct acgtcgacca
      661 ggtgctcgag acgtcggtcg agcggctgca ccttccggcc ggcgtcgacg tggcacggac
      721 gctcgcgtcc cccggcgcgg gccgcttcgt cgccggcctg cgtggccagg tcgtccagcg
      781 ccgtcacacg tcgccgctcc cgctctacga gcgcaccctc gagcgctgga acgggccggt
      841 caccgacctg gacctgctcg tcaacgcgta ctggaccgtc tcgccggtcg cctaccgccc
      901 gctgcccggc ggccgcgtcg ccccggtccc gaccaccaac cccgacgacg tgtggcggct
      961 ccgcttcagc gggacggtgc ggctgccggg cagcgacgac gtccagtacc gcgcggtgca
     1021 cctgcacgac ggctccaccg acgtggacca ggccggcgtg gtgcgtgcgc ccgccgtccg
     1081 cgtcgtgggg cgcttcgacc cggagcggct tcccggcttc aatccgttgt cccaggtccc
     1141 gctcgagacg tactacccgc ccgaggccga gggggccgac gcggccagcc ggaaggccct
     1201 gggcggccgg ccgttgcgcc ccacgaccaa catcggcggc tacctcgccc agccgccgct
     1261 gctcctcacc accatccagg cgctcgacgc gatgaccgac cccgccacct acccgacggc
     1321 cgaccacgcc gcgccgatca gcgtgatccg catccgggtg gcgggcgtga ccggtcccga
     1381 ccgggtcagc cgggagcgca tccggcgggt cgccgccgcg gtcgtcgcga ggaccggcct
     1441 cgcggtcgac gtgacggccg gctcctcgcc gcactccctg ctggtcgacc tgcccgccgg
     1501 cagggacggg cagccgcccc tgctcgtcag cgagggctgg gtcaagaagg gcgccgcgat
     1561 cgtgatcctg gcggcgctcg accgcaagag cgtggcgctg ttcttcctcg tgctgctgac
     1621 ctgcgcctgc ttcgtcgcca acggcgcgtt cgcggccgtg cgggcgcgga ggaccgagat
     1681 cggcaccctc cgctgcctcg gctggtccgc gggacgggtc ttcgctgtcg tcctcggcga
     1741 ggtcgccgcg atcggcctcg ccgccgggat cctcggcacg gcccttgccg ccgccggtgt
     1801 gcgcgtcctc cacctgcatc tggcgccgag ccagatgctg ctggtcgccc cggtcgccgt
     1861 ctggctcgcg gtgctggccg gcgtgcctcc ggcctgg
//
LOCUS       DASPYY010000512         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2614179, whole genome shotgun sequence.
ACCESSION   DASPYY010000512 DASPYY010000000
VERSION     DASPYY010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614179"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..557)
                     /locus_tag="VEP73_08135"
     CDS             complement(168..557)
                     /locus_tag="VEP73_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT26430.1"
                     /translation="MAPGAESRVIDHVGLLVRDLAASRRFYLAALGPLGFRLLYEQGP
                     SVAFGVEGHDDFAINASDTPGSHAHVAFVARDRAAVDAFHAAALANGGRDNGAPGLRP
                     EYHAGYYAAFVLDPDGNNVEAVHHDRG"
     gene            complement(581..1510)
                     /locus_tag="VEP73_08140"
     CDS             complement(581..1510)
                     /locus_tag="VEP73_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYT26431.1"
                     /translation="MRALVADPTASPALKVADVPEPTPGPGQVLVRMQAASVNRGEIR
                     NAGKQPPGTVIGWDVAGTVETLGEGVREFDVGQRVVGLNPAGGAFAELVVTPAEWTAP
                     LPEACDAVQASTLPVAGITAVGILRLARVHAGDRVLVTGAAGGVGQLAVQLARETKAT
                     VTGQAASEERAAAVREAGVEALVHPGDGSDVHGEFDVVLDGIGGPMVGPLLRATAPQG
                     RVVIYGNSADAPSTFRVEDFYNKGVTISGFRIFASVPPDQAVRDLASLADQVVAGRLD
                     VRVQATAPLADALDLVRDLYDRKVTGKVVVTAS"
     gene            1726..>2749
                     /locus_tag="VEP73_08145"
     CDS             1726..>2749
                     /locus_tag="VEP73_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT26432.1"
                     /translation="MKVTGAQALIRSLERCDVDVVFGLPGGAILPAYDPLRESSVRHI
                     LVRHEQGAGHAAEGYAWATGRVGVCMATSGPGATNLVTALCDAYMDSVPLVAITGQVP
                     SGAIGSDAFQEADITGITLPVTKHNELVTNPARIPSAVAEAFYIASTGRPGPVLVDIP
                     KDVLQATTEFAWPGSVDLPGYRPTVRPHAKRVREAAAMLREARQPVLYVGGGVIKAGA
                     HEALRELAEAAQAPLTTTLMARGAFPDTHPLALGMPGMHGNYAAVAALQEADLIVALG
                     ARFDDRVTGNLATFAPRARIVHADIDPAEIGKNRVVDVPIVGDLKLVLEELVAAYRAA
                     VAEAGPA"
BASE COUNT          356 a         1038 c         1000 g          355 t
ORIGIN      
        1 gctcgagcca tggaacggca tcgtccagcg ggtcgcggtg acggtgccgc tcggcttcat
       61 ggccgcgctc gcgtggcacc tgctccgccg gccgcgggcg tgagccggcc cgggacgagc
      121 cggcccgtcc gcgcgtcccg ccgcccgtgg cggccgggtt ggctcggtca accgcggtcg
      181 tggtggaccg cctcgacgtt gttgccgtcg gggtcgagga cgaaggcggc atagtagccg
      241 gcgtggtact ccgggcggag accgggcgcg ccgttgtcgc gcccgccgtt cgcgagcgcc
      301 gccgcgtgga acgcgtccac cgcggcccgg tcgcgggcga cgaaggcgac gtgcgcatgg
      361 cttcccggcg tgtcgctcgc gttgatggcg aagtcgtcgt gcccctcgac gccgaacgcg
      421 acgctcggcc cctgctcgta gagcagccgg aagcccaggg gcccgagcgc ggccaggtag
      481 aagcgccggc tcgccgcgag gtcgcgtacg agcaggccga cgtggtcgat cacccgggac
      541 tccgcaccag gcgccatgat gctccttccg tgacgcgggc tcaggacgcg gtgacgacca
      601 ccttgccggt caccttgcgg tcgtacaggt cgcgaaccag gtcgagggcg tcggccagcg
      661 gcgcggtggc ctgcacgcgc acgtccagcc ggcccgcgac cacctggtcg gccaggctgg
      721 ccaggtcccg gacggcctgg tcgggcggga cgctcgcgaa gatccggaag ccggagatcg
      781 tcacgccctt gttgtagaag tcctccaccc ggaaggtcga cggcgcgtcg gccgagttcc
      841 cgtagatcac cacccggccc tgcggcgcgg tcgcgcgcag cagcgggccg accatggggc
      901 cgccgatgcc gtcgagcacg acgtcgaact cgccgtgcac gtccgacccg tcgccagggt
      961 ggacgagcgc ctcgaccccc gcctcgcgca ccgcggcggc gcgctcctcg ctcgccgcct
     1021 ggccggtcac ggtcgccttc gtctcgcgcg cgagctggac ggcgagctgc ccgacgccgc
     1081 cggccgcgcc ggtgacgagc acgcggtcgc cggcgtggac ccgcgcgagg cgcaggatgc
     1141 cgacggctgt gatcccggcc accggcaggg tggaggcctg cacggcgtcg cacgcctcgg
     1201 gcagcggcgc ggtccactcg gccggggtca cgaccagctc ggcgaacgcg cccccggccg
     1261 ggttgagccc caccacccgc tggccgacgt cgaactcgcg cacgccctcg ccgagcgtct
     1321 ccaccgtccc ggccacgtcc cagccgatca ccgtgcccgg cggctgcttg ccggcgttgc
     1381 ggatctcgcc ccggttgacc gaggcggcct gcatgcggac gagcacctgc cccgggcccg
     1441 gggtcggctc gggcacgtcg gcgaccttga gagcggggga ggcggtggga tcggcgacca
     1501 gtgcgcgcat cggcggctcc tcgggcgggt tgacaggtcg ggtactatgc aacgcaatgc
     1561 cgccgatcat tgcagaggcc cgaatgccct gtcagctcca cctcgtactt atcacctagc
     1621 gcggacccga gtccggggtc cgcgcgaagc ctcctatgcc cgacggcagg aggttttttt
     1681 cttgccagaa accctgaacg cccagcaccc acggggagtc gagctgtgaa ggtcaccgga
     1741 gcgcaagccc tcatccgcag cctggaacgt tgcgacgtgg acgtcgtctt cgggcttccc
     1801 gggggcgcca tcctccctgc ctacgacccc ctgcgcgagt cctcggtccg ccacatcctg
     1861 gtccgccacg agcagggcgc cggccacgcc gccgagggct acgcctgggc gaccgggcgg
     1921 gtgggggtct gcatggccac gagcggcccg ggggcgacga acctcgtgac cgcgctgtgc
     1981 gacgcctaca tggactcggt gccgctcgtc gcgatcaccg ggcaggtgcc gagcggcgcc
     2041 atcggctcgg acgccttcca ggaggccgac atcaccggca tcacgctgcc ggtgaccaag
     2101 cacaacgagc tggtgaccaa cccggctcgc atcccgtcgg ccgtcgccga ggcgttctac
     2161 atcgcctcga ccgggcggcc cgggccggtc ctggtcgaca tcccgaagga cgtgctgcag
     2221 gcgaccaccg agttcgcctg gcccgggtcc gtcgacctgc ccggctaccg gccgaccgtc
     2281 cgcccgcacg ccaagcgggt gcgcgaggcg gccgccatgc tccgcgaggc gcgccagccc
     2341 gtcctctacg tggggggcgg cgtgatcaag gcgggggccc acgaggccct gcgcgagctc
     2401 gcggaggcgg cccaggcccc gctcacgacg accctgatgg cgcggggcgc gttcccggac
     2461 acccatccgc tggcgctcgg catgcccggc atgcacggca actacgccgc ggtggccgcg
     2521 ctccaggagg ccgacctgat cgtggcgctc ggcgcgcgct tcgacgaccg cgtgaccggc
     2581 aacctggcca ccttcgcgcc cagggcgcgc atcgtccacg ccgacatcga cccggccgag
     2641 atcggcaaga accgcgtggt cgacgtgccg atcgtgggcg acctcaagct cgtcctcgaa
     2701 gagctcgtgg ccgcctaccg ggcggccgtg gcggaggcgg ggccggccg
//
LOCUS       DASPYY010000513         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2193856, whole genome shotgun sequence.
ACCESSION   DASPYY010000513 DASPYY010000000
VERSION     DASPYY010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193856"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VEP73_08150"
     CDS             complement(<1..212)
                     /locus_tag="VEP73_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYT26433.1"
                     /translation="MDEGFRVVAVTAGDLRRLNRRWGEVVVPKVGWVRFRLTRPLGKH
                     GMARVTKDRASRWHVSFSAPQPPVVRT"
     gene            complement(508..564)
                     /locus_tag="VEP73_08155"
                     /pseudo
     CDS             complement(508..564)
                     /locus_tag="VEP73_08155"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1473..2183
                     /locus_tag="VEP73_08160"
     CDS             1473..2183
                     /locus_tag="VEP73_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26434.1"
                     /translation="MPTLAAGALNLGSALLPAGRQRLDLLDRLVPGAFSRGSTVATAA
                     AGIGLLLLAGGRRRRTAWLAVVVLLSASAVLHLLKGLDFEEAAVEAFLAGLLVGQGGR
                     FAARAGPGERRATAATALGAVVASVAYGALGLLANGRDMVEDLSVGRVLREVGRMALG
                     LGTTLTLTGRFGRFFPTSVSAVFWLGVLVVLARALAPALVRHAADPGLRAAVAAADDS
                     LAYFALRDDQATVRGAAR"
     gene            2146..2628
                     /locus_tag="VEP73_08165"
     CDS             2146..2628
                     /locus_tag="VEP73_08165"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HYT26435.1"
                     /translation="MTIRRRSGGPRADLVPAGRHGRARRRGSLGPREARPMGAQAFLA
                     EAAAKGRIAAVLVCGAEAARVWTAAGLTTMYLGDEAVLDLERFSLEGRALRIARQSWN
                     RARRAGFTALACRSGDLDPATVAALREVSRRWRGEATERGFSMALSRFVRPARRPPAT
                     "
     gene            3017..3331
                     /locus_tag="VEP73_08170"
     CDS             3017..3331
                     /locus_tag="VEP73_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26436.1"
                     /translation="MRIPSDLLHQLGGAARWKAPGSRDLLHGDPGGPVDWFRGAEVQD
                     QMDALLRYHLIQPMLVVAPDASGGWLHDSEMLNQVGGAQVETYLTRTVVEPGPRSRVA
                     NS"
BASE COUNT          398 a         1261 c         1340 g          475 t
ORIGIN      
        1 gtgcggacga cgggcggctg gggggcggag aacgagacgt gccagcggct ggcccggtcc
       61 ttggtgaccc gggccatacc gtgcttccca agcgggcggg tgaggcggaa gcggacccag
      121 ccgaccttgg ggaccaccac ctccccccag cggcggttca ggcggcgcag gtccccggcg
      181 gtcacggcga cgacgcggaa cccctcgtcc acccccgcgc ctgcgccacg acggcgtgcc
      241 aggcggcgcc ggccgctccc cgggtgggac gccggcgacc cgcgtgcgcc acgcccgcag
      301 cgcggcgacg tggctcgccc acgcctggtg gaagtcccgc agggcctgct gctggacgat
      361 cacgctcccc tcggccagcc agccgaacgc ggcgcgggcc tcggtgagct gccggcactg
      421 ctcggcgaat ccgggcgccg gccggccggg ctgccaaaac ccccgctgct ggaccgcgag
      481 gttccacacg tagcgggcgt gggcgcagtg gccgagcagc agggcttcct gcgcgggtgt
      541 ggggtccagc cggtagcggc tcatgcgcgg accctagcag ccacctgcga ccacgacgct
      601 gcctgccgtg ccgcatgccg cccccgcctg aaggcgcggg catcccgcgg tccacgaacc
      661 agtaagcatg ccagcctggc gagcgtccgg aacgccggcc ctagcagcgc ggcggaagcg
      721 ccccgaggcg cacgcttgcg gtctcgggtg atccgggtga tcgtctccag ccgctgcagc
      781 gggtcgccgg tcgcgggaag cgcgaccggg gtcacgccaa cctggttgcc gagctccgtg
      841 gcagtggcct gatgcctggc cgagacgggt acggagacga ccaacgtgtc gaccgtctcc
      901 ccgcggtggc agtacggcgt ggagggcgcc tgtcagcgcg gtgagcacga cgtcgttgac
      961 cgtccccccg tgcgcatgcg ccgtctcgcg gatctcggcg aggtccgcgt gccacggcca
     1021 ggctgcggcg tggtccggat ggtcggttca gcgagcacct gggcggccgg gcggtgccgt
     1081 cgggtcggac ctcggcgccg gcaccccgga ggcgtcgcac ccggacggca ggcgagcgag
     1141 gacccggagc cgggatacga gggcgcctgg gccgagcttc gatgccgcag gattgcagca
     1201 cccatcagac cccctccgct cgtcaaggga cgcacccgtc caaccgatcc agagggcagc
     1261 gtgtccccgg ccgggccgca ggaccagggt cgatggtccc gcgttccgcc tgccttcgtg
     1321 ccctgccaga ggtcagatca ggtcgtatgg tggtaatgga aggggtttgg ggaccgagag
     1381 cgagacggtc ctgtcccggg ggctttggga gatgggaggt accacgagcc gcaccgggtc
     1441 acccggtagg ggggcggacc cggtggacgg tggtgcccac gctcgcggcg ggcgcgctga
     1501 acctcgggtc ggcgctgctg cctgccgggc gccagcggct ggacctgctc gaccggctgg
     1561 tgcctggggc gttctcccgg ggctcgacgg tcgcgacggc ggccgccggc attgggttgc
     1621 tgctgcttgc cggcgggcgg cgacggcgga cggcctggct tgcggtcgtc gtcctgctga
     1681 gcgcgagcgc ggtcctgcac ctgctcaagg gcctggattt cgaggaggcg gcggtcgagg
     1741 cgttcctggc ggggctgctc gtcggccagg gcggacggtt cgcggcgcgg gcgggcccgg
     1801 gggagcgccg cgcgacggcg gcgaccgcgc tcggggcggt cgtggccagt gttgcctacg
     1861 gcgcactcgg gctgctcgcc aacgggcggg acatggtcga ggacctcagc gtcggccgcg
     1921 tgctccgcga ggtcggacgc atggcgcttg ggctcggcac cacgctgacc ctcaccgggc
     1981 ggttcggacg attcttcccg acctcggtct cggccgtgtt ctggctgggc gtgctcgtcg
     2041 tgcttgcccg tgcgctcgcg cccgcgctcg tccgccacgc ggccgacccc ggcctgcgcg
     2101 cggcggtggc cgccgccgac gactcgctcg cgtacttcgc gctgcgtgac gatcaggcga
     2161 cggtccgggg ggccgcgcgc tgatctcgta ccggccggtc ggcacggtcg cgctcgccgc
     2221 cggggatccc tgggtccgcg ggaggcgcgg ccgatgggcg ctcaggcgtt cctggccgag
     2281 gcggccgcca aggggcggat cgcggcggtg ctggtctgcg gcgcggaggc cgcccgggtg
     2341 tggaccgcgg ccgggctcac cacgatgtac ctgggtgacg aggccgtgct cgacctcgag
     2401 cgcttctcgc tcgagggacg ggcgctgcgg atcgccaggc agagctggaa ccgggcccgt
     2461 cgcgctggct tcaccgccct ggcctgccgg tctggcgacc tggacccggc caccgtggcc
     2521 gcgctgcggg aggtctcgcg gcgctggcgg ggcgaggcga ccgagcgcgg cttctcgatg
     2581 gcgctcagcc ggtttgttcg acccgcgcga cgccccccag ctacctgaac gacttcctgg
     2641 tcgtcgaggc ggcccgccgc ccgccgggtc tgggcgtgcg gcgactgtcg ctgaacctct
     2701 cattcccgcg cggtgctcgt ggccggcgcc gagccagacg tgccgctcgg gctgcgcgcg
     2761 gcgcgctggc tgctccgccg cctgtccggc ccgttccagg tcgagacgct gtaccggttc
     2821 aacaagaagt tcgcccccgt ctggcagcca cgctacctgg cggtcgaggt cccagaggaa
     2881 ctgccccggg tcgcgctcgc cgtcgtacgc gccgaggggc tgctgccagc ggcaccatgg
     2941 cgcctcgccg ggcccccggc cgcgccaccc ggcgggtcgc accgccccac cggctcggcc
     3001 ccgcgtcgtg cgcctggtgc gcatcccgtc cgacctgctc caccagctcg gcggagcggc
     3061 ccgctggaaa gcccctggct cgcgggacct cctccacggc gacccaggcg gcccggtgga
     3121 ctggttccgc ggcgccgagg tgcaagacca gatggacgcg ctgctgcgct accacctcat
     3181 ccagccgatg ctcgtcgtcg cgcctgacgc cagcggcggc tggctgcacg actccgagat
     3241 gctcaaccag gtcggcggcg cgcaggtcga gacctacctc acccgcacgg tcgtcgaacc
     3301 cggcccccgc tcgcgggtcg ccaactcctg aacgcgggga gatgaccgga tatgcgacca
     3361 gctctgccgg tcgagctgtg gggagtggcg tgaggcggcg ggccaggaac gatggagggt
     3421 tgtccgtgcc ggtgtggtgg tccagcatgc agccggcggc gatgccggtc gcct
//
LOCUS       DASPYY010000514         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1914874, whole genome shotgun sequence.
ACCESSION   DASPYY010000514 DASPYY010000000
VERSION     DASPYY010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914874"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VEP73_08175"
     CDS             <1..1116
                     /locus_tag="VEP73_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT26437.1"
                     /translation="HAGFLVKVASEVAYGFELAPGRTFCWITDMGWIMGPLSIFGAHA
                     CGGTLLLYEGSPDVPGTSRLWDLVERHGVSTLGVSPTLVRTLRAAGARPAGDLSSVRV
                     LGSTGEPWDPESYDWLARDVFGGRVPVINFSGGTEVGGSFLCPYPVEEIRSCSLGGPA
                     LGMDVDVVDDVGRSLRGSVGELVCRQPWPSMTRGVWKDDERYREAYWSTYPGLWRHGD
                     YALADDTGWFILGRSDDVMNVAGKRVAPAEIEAVLATDAAVAESAVVGIPDATKGEAI
                     WAFWVRRAGATDGGGGDGDDGVVSARLTALVAAEVGKPFAPARVVRVGQLPKTRSAKI
                     MRRAIRAAVLDTDPGDLSGAENPEAVQDIRSQVKELG"
     gene            1116..>1536
                     /locus_tag="VEP73_08180"
     CDS             1116..>1536
                     /locus_tag="VEP73_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT26438.1"
                     /translation="MRWQLSEEQEAYRQSFGAWLADVAPREAMRRWLGDGDAAEFAGR
                     LVRDGWAGVGVPEALGGQGGGLVELALTAELLAGASAPSATWLATALAVPALGSGTDV
                     AAFGGEHVALLSPADAIPGAGPRLTIDGEGRLHGTVPR"
BASE COUNT          177 a          533 c          599 g          227 t
ORIGIN      
        1 catgccggct tcctcgtcaa ggtggccagc gaggtcgcct acggcttcga gctcgcgccc
       61 ggccggacgt tctgctggat caccgacatg ggctggatca tggggccgct gtcgatcttc
      121 ggcgcgcacg cctgcggcgg caccctgctg ctctacgaag gctcccccga cgtcccgggc
      181 accagccggc tctgggacct cgtcgagcgg cacggcgtgt cgacgctcgg cgtctcgccg
      241 acgctggtcc ggacgttgcg ggccgccggc gcccgtccgg ccggtgacct gtcctcggtc
      301 cgggtcctgg gatcgaccgg cgagccctgg gaccctgagt cctacgattg gctggcccgg
      361 gacgtcttcg gcgggcgggt ccccgtcatc aacttctccg gcggcaccga ggtgggcggc
      421 tcgttcctgt gcccctaccc ggtggaggag atccgcagct gctccctggg cggtccggcg
      481 ctgggcatgg acgtcgacgt ggtcgacgac gtcggccggt cgctgcgcgg cagcgtcggc
      541 gagctcgtct gccgccagcc ctggccgtcg atgacgcgcg gggtctggaa ggacgacgag
      601 cggtaccgcg aggcctactg gtcgacgtac cccggcctgt ggcggcacgg ggactacgcc
      661 ctggcggacg acaccggctg gttcatcctc ggccgctccg atgacgtgat gaacgtggcc
      721 ggcaaacgcg tcgccccggc ggagatcgag gcggtgctcg cgaccgacgc cgccgtcgcc
      781 gagtccgcgg tcgtcgggat cccggacgcg acgaagggcg aggcgatctg ggccttctgg
      841 gtccgacggg ccggcgcaac ggacggcggc gggggcgacg gggacgacgg ggtggtgtcg
      901 gcgcggctca ctgcgctggt tgccgccgag gtaggcaagc cgttcgcacc tgcgcgcgtg
      961 gtccgcgtcg gacagctgcc gaagaccagg tcggccaaga tcatgcgccg ggcgatccgc
     1021 gcggccgtgc tcgacactga tcccggggat ctgtccgggg ccgagaaccc cgaggccgtc
     1081 caggacatcc ggtcccaggt caaggagttg ggctgatgcg gtggcagctg tccgaggagc
     1141 aggaggccta ccggcagtct ttcggcgcct ggctcgccga cgtggcccca cgcgaggcga
     1201 tgcgccggtg gctcggtgac ggtgacgcgg cggagttcgc cgggcggctg gtccgcgacg
     1261 gatgggccgg ggtcggcgtg ccggaagccc tgggcggtca gggcggcggg ctcgtcgagc
     1321 tggccctcac ggccgagttg ctcgccgggg cgagtgctcc gtccgcgacc tggctggcga
     1381 ccgcgctcgc ggtacccgcg ctcggctccg ggaccgacgt cgccgccttc gggggtgagc
     1441 acgtagcgtt gctgtccccg gccgacgcca tcccgggtgc gggtccccgc ctcacgatcg
     1501 acggcgaagg gcgtctgcac gggacagtcc cgcggg
//
LOCUS       DASPYY010000515         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2193923, whole genome shotgun sequence.
ACCESSION   DASPYY010000515 DASPYY010000000
VERSION     DASPYY010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193923"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VEP73_08185"
     CDS             <1..179
                     /locus_tag="VEP73_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYT26439.1"
                     /translation="LGGVAWWAAVRLGRQARPTPTLVRLYDRLVVPAERALERRFQPR
                     FGQSVLCVARVPEA"
     gene            complement(236..1057)
                     /locus_tag="VEP73_08190"
     CDS             complement(236..1057)
                     /locus_tag="VEP73_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26440.1"
                     /translation="MRRVVGPAQGGDRLVDLTAAEAQGACLAVLSEGEVPLGHWAAEL
                     RRAAIAEPGRVVYGAVAEQRVAPEDWQGRRGHAAVGPVSSPENPRFDLVEHLGHLATF
                     AASGGVAVPRELLAELSPPEDGPGWDAWAVLLRGALRHGVASTGSVIALRRRWDGVGG
                     PAAARRAALLARLDTQGVAVLPGLLQRVQRLAATRDELLARVAAVEREQAEAAAAVTR
                     REDQLRRERDEATRALADARRAVEELRHSTSWRLTEPVRAAGGLARRLGRRDVRR"
     gene            complement(1265..>2038)
                     /locus_tag="VEP73_08195"
     CDS             complement(1265..>2038)
                     /locus_tag="VEP73_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT26441.1"
                     /translation="HMVACWLGDCSLEPCLDPGELLAARRRYASLDRLEHRFFGDNLG
                     HGGGHNRLLVEDAMEDVTLLVNPDTYPSPWLLVELLDGLADPSTAAVEGRQLPFEHPK
                     RYDPGTGLTGWASGACSLVRTTVLGEVGGFDADSFFLHGDDVDLSWRIRLAGHRIRHR
                     PAAVVFHDKAPRDGIPAQGMAERVHSAEAAIMLGWKWSRPDVVAHWSRWFAASGDPAY
                     EEAARRFQTRIEEGRLPAQLDPDGTIGEFVESYYAVHRF"
BASE COUNT          288 a          751 c          741 g          258 t
ORIGIN      
        1 tcctcggcgg cgtcgcctgg tgggccgcgg tccggttggg gcgccaggcg cggcccacac
       61 cgaccctggt ccgcctgtac gaccgcctgg tggtccccgc cgagcgcgcc ctcgagcggc
      121 ggttccagcc ccggttcggc cagtcggtcc tctgcgtcgc ccgggtgccc gaagcctgaa
      181 tcctccgatg agggactgcg ctgctgaggc ggcatgtcgc agggaccgtc gcgaatcatc
      241 gacggacgtc ccgccgcccc aggcggcgcg ccagcccgcc agccgcgcgc accggctcgg
      301 tcagtcgcca gctcgtcgag tggcgcagct cctcgacggc ccgccgtgcg tcggcgagag
      361 cccgcgtggc ctcgtcgcgc tcgcgtcgca gttggtcctc acgtcgagtc accgcggccg
      421 ccgcctctgc ttgctcgcgc tcgacagcgg cgacccgagc gagcaactcg tcacgcgttg
      481 cggccaggcg ctgcacgcgc tggagcaggc ccggtaggac agcaaccccc tgggtgtcga
      541 gcctcgccag cagggccgca cgccgcgcag cagcgggccc gccgaccccg tcccagcggc
      601 ggcgcagtgc gatcaccgag ccggtgcttg cgacgccatg gcgcagcgcg ccgcggagca
      661 gcacagccca ggcgtcccat ccggggccgt cctcaggcgg cgagagctcg gcgagcagct
      721 cgcgggggac ggccacgccg cccgaggccg cgaacgtggc caggtgtcca aggtgctcga
      781 cgaggtcgaa ccgcgggttc tccggggagg acacgggacc cacggcggca tgcccgcgcc
      841 gcccctgcca gtcctcgggc gcaacgcgct gctcggccac tgcaccgtag acgacccggc
      901 cgggctcggc gatggcagcc cgccgcaact cagcggccca gtggccgagc ggcacctcgc
      961 cctcggacag gacggcgagg caggcgcctt gggcttcggc ggcggtgagg tcgaccaggc
     1021 ggtccccgcc ctgcgcgggt ccgacgacgc ggcgcacccg tgcccggaag tccttcgcga
     1081 aggccccgac aagctcctcc ggggctcctc cgctctcgcg gtcagcgtcg gctaggagga
     1141 gcacttcgag gtcatcgtcg gtctgagcgg cgagcgcgag gagcgtgtca tgcagcagcc
     1201 atgcccggtc gccatgggca tgcaccacga cgctcagaac gggcaggttg gcggcaggca
     1261 tggctcagaa ccggtgaacc gcgtagtagg actcgacgaa ctcgccgatc gtgccgtccg
     1321 ggtcgagctg tgcgggtagt cggccctcct cgatacgggt ctggaaccgg cgggccgcct
     1381 cctcgtaggc ggggtcgccg ctcgccgcga accagcgcga ccagtgtgca acgacgtccg
     1441 ggcgcgacca tttccagccg agcatgatcg ccgcctcggc cgagtgcaca cgctcggcca
     1501 tgccctgcgc agggatgccg tcccgtggcg ccttgtcgtg gaacacgacc gcagccggcc
     1561 ggtggcgaat gcggtgaccg gcgagccgga tacgccagga caggtccacg tcgtcgccat
     1621 gcaggaagaa cgagtcggca tcgaacccgc cgacctcgcc cagcacggtg gtgcgtacca
     1681 gcgagcacgc gcccgacgcc cacccggtca ggccagtccc ggggtcgtag cgcttggggt
     1741 gttcgaacgg gagctggcgg ccctcgacgg ccgcggtgga agggtcggcc agaccgtcga
     1801 gcagttcgac cagcagccac gggctggggt aggtatcggg attgaccagc agggtcacgt
     1861 cttccatggc gtcctcgacc aacaggcggt tgtgcccgcc gccgtggccg aggttgtccc
     1921 cgaagaagcg gtgctcgaga cggtccaggc tcgcgtaccg acggcgcgcg gcgagcagct
     1981 cgccgggatc gaggcagggc tccagggagc agtcacccag ccagcacgcc accatgtg
//
LOCUS       DASPYY010000516         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1915119, whole genome shotgun sequence.
ACCESSION   DASPYY010000516 DASPYY010000000
VERSION     DASPYY010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915119"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1352)
                     /locus_tag="VEP73_08200"
     CDS             complement(21..1352)
                     /locus_tag="VEP73_08200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT26442.1"
                     /translation="MNGYDYDVIVLGAGSPGEHCAGELAGGGLKVAVVERELAGGECS
                     YYACIPSKTLLRPGEATHGAIEAAATAQVDVEAALAYRDYMVSNYSDAGQERWLADNG
                     IDLIRGTGRLAGPGVVEVADRRYTARDVVLATGSDPVIPPVPGLRELDGVWTNREVTG
                     MKAVPRRLLILGGGPVGAEMAQAVHRLGGEVALVEGADHVLAREPKPLGDALGEELRR
                     DGIELILGVHATAARRDGEDYVLTLGDGRELRGDRVLVATGRRPRPPEGLDTVGVTAG
                     PHGVPVDAHLRVGERLWAIGDVTGIRLLTHVGKYQGEVVASNLLGEPREAHYEAVPDV
                     VYTDPEAAAVGASEAPFSGTAMLSEVAKMETYTHAYAESKGFMTLMSDGERLTGAYAL
                     GPAAGEWLQQATLAIRARVPLDVLRDVIQPFPTFSEIYVEALKALRGAIAS"
     gene            complement(1349..>1641)
                     /locus_tag="VEP73_08205"
     CDS             complement(1349..>1641)
                     /locus_tag="VEP73_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26443.1"
                     /translation="ACIPSKTLLRPGEATHGAIEAAATAQVDVKAALAYRDFMVSNYS
                     DAWAARWLADALRRAEAAHGKYEQETGKPDPDWPAWYARYMADEAAGEGARK"
BASE COUNT          196 a          626 c          586 g          233 t
ORIGIN      
        1 ctgcccggtc agttgagtgg tcatgatgcg atcgctccgc gcagggcctt cagcgcctcg
       61 acgtagatct cggagaaggt ggggaacggc tggatgacgt cgcgcagcac gtcgagcggg
      121 acgcgggcgc ggatggccag ggtggcctgc tgcagccatt cacccgccgc gggacccagg
      181 gcgtaggcgc cggtcagccg ctcgccgtcg gacatcagcg tcatgaagcc cttagactcg
      241 gcgtaggcgt gggtgtacgt ctccatcttg gctacttcgg acagcatggc ggtcccgctg
      301 aacggggcct cgctggcgcc gacggcggcg gcctccgggt cggtgtaaac cacgtccggg
      361 acggcctcgt agtgcgcctc gcgcggctcg cccaagaggt tcgaggcgac gacctcgccc
      421 tggtacttac ccacgtgggt cagcagccgg atcccggtga cgtccccgat ggcccacagc
      481 cgctccccga cgcgcaggtg cgcgtcgacc ggcaccccgt gcggcccggc ggtcaccccg
      541 accgtgtcca ggccctcggg cggccgcggg cgccggccgg tggccacgag cacgcggtcg
      601 ccgcgcagtt ccctgccgtc gcccagggtc aggacgtagt cctcgccgtc acggcgggcg
      661 gcggtcgcgt gcacgccgag gatcagctcg atgccgtccc ggcgcagttc ctcgccgagc
      721 gcgtcgccca gcggtttggg ctcgcgggcc agcacgtggt ccgcgccctc gaccagggcg
      781 acctcaccgc ccaggcggtg cacggcctgg gccatctcgg cgcccaccgg gccgccgccc
      841 aggatcagca ggcggcgggg gaccgccttc atcccggtca cctcgcggtt ggtccacacc
      901 ccgtccagct cgcgcaggcc cgggaccggc ggtatcaccg gatccgagcc ggtcgccagc
      961 accacgtcgc gggccgtgta gcgccggtcc gccacctcga ccacgcccgg gccggccagc
     1021 cggccggtgc cgcggatcag gtcgatgccg ttgtccgcca gccaccgctc ctgcccggcg
     1081 tcggagtagt tcgagaccat gtagtcccgg taggccagcg cggcctcgac gtccacctgc
     1141 gcggtggcgg ccgcctcgat cgcgccgtgg gtggcctcgc ccgggcgcag cagggtcttg
     1201 gacgggatgc aggcgtagta ggagcactcg cccccggcta gttcccgctc gaccacggcg
     1261 accttgaggc cgccgccggc cagctccccg gcgcagtgct cgccgggcga gccggcccct
     1321 agcacgatca cgtcgtagtc gtagccgttc atttgcgcgc gccctccccg gccgcctcgt
     1381 ccgccatgta ccgggcgtac caggccggcc agtccgggtc gggcttcccg gtctcctgct
     1441 cgtacttgcc gtgcgccgcc tcggcccggc gcagcgcgtc cgccagccac cgcgcagccc
     1501 aggcgtcgga gtagttcgag accatgaagt cccggtaggc cagcgcggcc ttgacgtcca
     1561 cctgcgcggt ggccgccgcc tcgatcgcgc cgtgggtggc ctcgcccggg cgcagcaggg
     1621 tcttggacgg gatgcaggcg t
//
LOCUS       DASPYY010000517         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2614976, whole genome shotgun sequence.
ACCESSION   DASPYY010000517 DASPYY010000000
VERSION     DASPYY010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614976"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..703
                     /locus_tag="VEP73_08210"
     CDS             38..703
                     /locus_tag="VEP73_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26444.1"
                     /translation="MIRVLLADDQVLVRAGFRALLDAQDDIEVVGEAGDGEEAVRLAG
                     RLVPDVVLMDVRMPGLDGLAATRMIAADARLAQVRVVILTTFELDEYVFEALRVGASG
                     FLVKDTEPVDLIRGVRAVARGDALLSPGVTRRLIAEFAARAKEPRTMPELDALTDRER
                     EVMALVAGGLSNEEIAERLVVSPATAKTHVSRAMVKLGARDRAQMVVLAFESGLVKPG
                     WLG"
     gene            937..1656
                     /locus_tag="VEP73_08215"
     CDS             937..1656
                     /locus_tag="VEP73_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26445.1"
                     /translation="MHATKRYGSGDTSVLALDDVTVDLPGGRFTAVMGPSGSGKSTLL
                     HVVAGLDTLTSGQVFLGGVELGSLSDKELTLLRRERVGFVFQAYNLLPTLTALENVTL
                     PLALAGTRPDREWLDGVIDTVGLRARLSHRPAELSGGQQQRVAVARALVSRPQVVFAD
                     EPTGNLDSRSGAEILDFLRRAVRELGQTVVMVTHDPVAAGYADAVLFLADGRIVDRME
                     GPTPQRVLDRMKALGGEWRST"
     gene            1656..>2765
                     /locus_tag="VEP73_08220"
     CDS             1656..>2765
                     /locus_tag="VEP73_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYT26446.1"
                     /translation="MLKLSLKGLWAHKRRLAGTFSAVLLGVAFLAGALVLSDTLRASI
                     DGFFTDSNKGTDVVVRNATLVTDNAGAQRGPVDGSLVDRVRAVDGVAVAEPVVDGFGQ
                     PVGRDGQAVTGNGPRTAGNWVADPELNPYRIAEGRPPRTDDEVVINRGAAKDGDLHVG
                     DRTTVLTPEPVAVTVVGIATFGSADAYGGTSFTAFTLAGAERHVARQPGKVTRIAVRG
                     VPGVPQDELVRRVRAVLPRGVEAIGGARLTEENLAQVDKQFLGLFRTFLTLFAGIALL
                     VATFSIYNTFSIIAAQRARESALLRAVGASRGQVLASVVTEALLVGLAASAAGLAGGL
                     GIAKLLQGAFAGLGFDLPATGLVLHASTVAVSVPTG"
BASE COUNT          338 a         1050 c         1013 g          364 t
ORIGIN      
        1 tccgggtgct cgcccgcctc cccctggacg gcaagcggtg atccgggtcc tgctcgccga
       61 cgaccaggtg ctggtgcggg ccggcttccg cgcgctgctc gacgcccagg acgacatcga
      121 ggtcgtcggc gaggcgggcg acggcgagga ggcggtgcgg ctcgcgggac gcctggtgcc
      181 cgacgtggtc ctcatggacg tccgcatgcc agggctcgac gggctcgcgg cgaccaggat
      241 gatcgccgcc gacgcacgcc tcgcccaggt ccgggtcgtc atcctcacca ccttcgagct
      301 cgacgagtac gtcttcgagg cgctgcgcgt cggcgcgagc ggcttcctgg tcaaggacac
      361 cgagccggtc gacctgatcc gcggggtacg ggcggtcgcc cgcggcgacg ccctgctctc
      421 cccgggcgtc acccgccggc tgatcgcgga gttcgccgcc cgtgccaagg agccccggac
      481 catgcccgag ctggacgccc tgaccgaccg ggagcgcgag gtgatggcgc tggtcgccgg
      541 cgggctgtcc aacgaggaga tcgccgagcg gctcgtggtg agcccggcga cggcgaagac
      601 ccacgtgagc cgggcgatgg tcaagctggg cgcccgcgac cgcgcgcaga tggtcgtgct
      661 cgccttcgag tccggcctgg tgaagcccgg ctggctcggc tgacccgcct acctccggcg
      721 gggtaggtcg cctggcgcgc gtgcgccccg gggcggcccc gcgaagcctc ccccgggacg
      781 acgacgccgg ggcgaccggg ccccaggatc ggcgggaacg caccgccaac cggaaaggcc
      841 ccggccatga ccacatcccc ggcaggctcc atggcgcagc gcccgtcgcc ggccaggtcg
      901 cccgggccgg cgacgggcac cccagtggcg cgtacggtgc acgcgaccaa gcgctacggg
      961 tcgggcgaca cctcggtcct cgccctcgac gacgtgaccg tcgacctccc cggcgggcgg
     1021 ttcaccgccg tcatgggccc gtcgggctcg ggcaagtcga ccctgctgca cgtcgtcgcg
     1081 ggcctcgaca cgctcacgtc cggccaggtg ttcctcggcg gcgtcgagct tggctcgctg
     1141 tccgacaagg agctcaccct gctgcggcgc gagcgcgtcg ggttcgtctt ccaggcgtac
     1201 aacctcctgc ccaccctcac cgcgctcgag aacgtcaccc tgccgctggc cctcgccggc
     1261 accaggcccg accgggagtg gctcgacggc gtcatcgaca ccgtcggcct gcgcgcccgg
     1321 ctctcgcacc ggccggcgga gctgtcaggc ggccagcagc agcgggtcgc ggtggcacgg
     1381 gcgctggtga gccgcccgca ggtcgtgttc gccgacgagc cgaccgggaa cctcgactcc
     1441 cggagcggcg cggagatcct cgacttcctg cgccgagcgg tccgggagct cggccagacg
     1501 gtcgtgatgg tgacccacga cccggtggcg gccggctacg ccgacgccgt gctgttcctg
     1561 gccgacggcc ggatcgtcga ccggatggag gggccgacac cgcagcgggt gctcgaccgc
     1621 atgaaggccc tcggcgggga atggaggtcg acgtgatgct gaagctcagc ctcaagggcc
     1681 tgtgggccca caagcgccgg ctcgccggca ccttctccgc ggtgctgctc ggcgtcgcgt
     1741 tcctggccgg ggcgctcgtg ctgagcgaca ccctccgggc cagcatcgac ggcttcttca
     1801 cggactcgaa caagggcacc gacgtggtcg tccgcaacgc caccctggtc accgacaacg
     1861 ccggtgccca gcgcggcccg gtcgacgggt cgctcgtcga ccgggtccgc gccgtcgacg
     1921 gcgtggccgt cgccgagccg gtcgtggacg gcttcggcca gccggtcggc cgcgacggcc
     1981 aggcggtcac cgggaacggg cccaggacgg cgggcaactg ggtggcggac ccggagctca
     2041 acccctaccg gatcgccgaa ggccggccgc cgcgcaccga cgacgaggtg gtgatcaacc
     2101 gcggcgccgc caaggacggc gacctccatg tcggcgaccg gaccacggtg ctcacccccg
     2161 agccggtcgc ggtgacggtc gtcggcatcg ccaccttcgg gtcggccgac gcctacggcg
     2221 gcacctcgtt caccgccttc accctcgccg gagccgagcg gcacgtcgcc aggcagccgg
     2281 ggaaggtcac ccggatcgcg gtgcggggcg tgccgggcgt cccccaggac gagctggtgc
     2341 gccgtgtccg cgccgtgctg ccccgtggcg tcgaggccat cggcggggcg cggctcaccg
     2401 aggagaacct cgcccaggtc gacaagcagt tcctcgggct gttccggacg ttcctcaccc
     2461 tgttcgccgg gatcgccctg ctggtcgcca cgttcagcat ctacaacacg ttctcgatca
     2521 tcgccgccca gcgcgcgcgg gagtccgcgc tgctgcgggc ggtcggcgcc tcgcgggggc
     2581 aggtcctcgc ctcggtcgtc accgaggcgc tgctcgtcgg cctggccgcg tccgccgccg
     2641 ggctggccgg cgggctcggc atcgccaagc tcctccaggg ggccttcgcc gggctcgggt
     2701 tcgacctgcc ggccaccggc ctggtgctgc acgcctcgac ggtggccgtc tcggtgccca
     2761 ccggg
//
LOCUS       DASPYY010000518         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_86664, whole genome shotgun sequence.
ACCESSION   DASPYY010000518 DASPYY010000000
VERSION     DASPYY010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86664"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VEP73_08225"
     CDS             <1..1112
                     /locus_tag="VEP73_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYT26447.1"
                     /translation="LLLALTAAPHAAAGYYDLQAYDLLTSIFHSRDEGWAASGSPAPG
                     PARASVPGRLTVLLLGGDAGYGRIGRRTDTMIVASVEVASGRATLFGLPRNLIRVPLP
                     VGPARAFDCRCFPHPLNELYAYGEAHPELFPGSRQPGVSAIMGAAEELLGIPIDHYAL
                     VDLRGFVKVVDALGGVTVTVAEPVHVEVDRLGEAAGGPSFHLKPGRHHLDGFTALAYA
                     RARKETSDYDRMRRQRCLLRSLARQADAGTLLRAFPRLVPVLKRNVVTDLPLDRLPDL
                     IELAGQHRVRLAAIGFTPPDYSAGWADGGYPVPDVRRIRAAARAMLEGRPAPVPASPG
                     ATAGAKPATGGGGAAAKPATGRTDQLFPRGPDTCG"
     gene            complement(1117..>1526)
                     /locus_tag="VEP73_08230"
     CDS             complement(1117..>1526)
                     /locus_tag="VEP73_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYT26448.1"
                     /translation="ERFLGELALGAEVDAWDPRADRISLLTLHAAKGLEFPAVFLTGC
                     EDGLLPLRWGGAAAGLDLAEERRLFFVGMTRARSHLFLSHARWRTRHGAAQEAAPSEF
                     LSDIGEALLERVRAEARTPRRRPLPAGDQLQLL"
BASE COUNT          185 a          608 c          559 g          174 t
ORIGIN      
        1 ccctgctcct cgcgctgaca gccgcccccc acgccgccgc cggctactac gacctccagg
       61 cctacgacct gctcacctcg atcttccaca gccgcgacga ggggtgggcg gcgtcgggct
      121 cgccggcgcc cgggccggcg cgggcgtccg tccccggccg cctcaccgtc ctgctcctcg
      181 gcggggacgc cgggtacggc cggatcggcc ggcggaccga cacgatgatc gtcgcgagcg
      241 tcgaggtcgc gtccggccgg gcgacgctgt tcgggctccc gcgcaacctg atccgggtgc
      301 cgctcccggt cggcccggcc agggccttcg actgccgctg cttcccgcac ccgctgaacg
      361 agctctacgc gtacggcgag gcgcaccccg agctgttccc cggcagcagg cagccgggcg
      421 tgagcgcgat catgggcgcg gccgaggagc tgctcggcat cccgatcgac cactacgcgc
      481 tcgtcgacct cagggggttc gtcaaggtgg tggacgcgct cggcggcgtg acggtgaccg
      541 tcgccgagcc ggtgcacgtg gaggtcgacc ggctcggcga ggccgccggg gggccgtcgt
      601 tccacctcaa gccagggcgc caccacctcg acgggttcac cgccctcgcc tacgcccggg
      661 cccgcaagga gaccagcgac tacgaccgca tgcgcaggca gcgctgcctg ctccggtcgc
      721 tcgccaggca ggccgacgcc ggcacgctgc tgcgggcctt cccccggctc gtgcccgtgc
      781 tcaagcgcaa cgtcgtgacc gacctcccgc tcgaccgcct ccccgacctg atcgagctcg
      841 ccggccagca ccgggtgcgc ctggccgcga tcggcttcac gccgcccgac tacagcgccg
      901 gctgggcgga cggcggctac ccggtccccg acgtgcgacg catccgggcc gccgcccgcg
      961 cgatgctcga ggggcgcccg gcccccgtcc cggcatcccc gggcgccacg gccggggcga
     1021 agcccgcgac cggtggcggg ggagcggcgg cgaagcccgc gaccggccgg acggaccagc
     1081 tgttcccccg ggggccggac acctgcgggt gagccgtcac aggagctgga gctggtcacc
     1141 cgctggcagc ggccgccggc gcggcgtcct ggcctcggcg cggacccgct ccagcagggc
     1201 ctccccgatg tcggagagga actccgaggg cgcggcctcc tgggccgcgc cgtgcctcgt
     1261 gcgccaccgg gcgtggctga ggaacaggtg cgacctggcc cgggtcatgc cgacgaagaa
     1321 cagccggcgc tcctcggcga ggtcgaggcc ggccgccgcg ccaccccagc gcagcgggag
     1381 caggccgtcc tcgcagccgg tgaggaacac ggccgggaac tcgaggccct tggcggcgtg
     1441 cagggtgagc agcgagatcc ggtccgccct cgggtcccag gcgtcgacct cggcgccgag
     1501 cgccagctcg ccgaggaagc gctcga
//
LOCUS       DASPYY010000519         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1213719, whole genome shotgun sequence.
ACCESSION   DASPYY010000519 DASPYY010000000
VERSION     DASPYY010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213719"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VEP73_08235"
     CDS             <1..673
                     /locus_tag="VEP73_08235"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HYT26449.1"
                     /translation="GWWQLHLPDPEWLRRGRGQRVDVVYEPGPGRVDGYATYGLRSRW
                     TDNIPDSIAQVMELMAVTPEAAASLWRFCLDLDLTTTVELRNRPLDEPLRWCLADPRR
                     LRTTALSDFLWLRVLDLPAALAARGYAAEGQLVLEVSDPLLPDNDGRFRLDGGPGGGR
                     CARTSFAPDVILDVAALGAAYLGGVRFSTLGRAGRVREGRPGALARADAMFASDPAPW
                     CSTDF"
     gene            784..1140
                     /locus_tag="VEP73_08240"
     CDS             784..1140
                     /locus_tag="VEP73_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26450.1"
                     /translation="MVTGAGRGLGAAYARLLAARGAAVVVHDAGVAEDGSGFDSSVAD
                     AVADEIVEWGGTATACHENLESGAGCRRVVELAAEQFGRVDVLVHNAGLVVFAPVEET
                     APAIAERWPELTAATC"
     gene            complement(1137..2096)
                     /gene="era"
                     /locus_tag="VEP73_08245"
     CDS             complement(1137..2096)
                     /gene="era"
                     /locus_tag="VEP73_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HYT26451.1"
                     /translation="MPVKSGLVALVGRPNVGKSTLLNGLLGQKLAIMSDKPQTTRAVV
                     RGVLHDERGQAVLVDTPGLHKPQTLLGQRLNDLVRGTLAEVDLVCLLVDAAAGVGRGD
                     RFLAEELARVGTPVVCVVNKMDAAPRAGLVRALEAAAALGDWAEVVPVSARTGEQLDV
                     LAGLLFDHLPEGPLLYPEGHVTDEPEQHLIAEVIREKALALVRDELPHSVAVLVDEVG
                     PAEAPEPRPDGLRPGQGRTARPAREPEDRDPLLVVRATLFVERASQKPIVLGRQGSVL
                     REIGTRARTELEALLGTRVYLDLHVKVAKEWQRDPRQLARLGF"
     gene            complement(2087..2515)
                     /locus_tag="VEP73_08250"
     CDS             complement(2087..2515)
                     /locus_tag="VEP73_08250"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HYT26452.1"
                     /translation="MTGLPTGELVAAARGARERAYAPYSGFRVGAALLAEGGDVVTAA
                     NVENASYGLGICAERAAVVAAVAAGYRRFEAIAVAGDGQAPVTPCGACRQVLREFPAA
                     LDLEVVCAGESGDQLLVTTLGALLPESFGPEQLAAPEACR"
     gene            complement(2512..>2847)
                     /locus_tag="VEP73_08255"
     CDS             complement(2512..>2847)
                     /locus_tag="VEP73_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HYT26453.1"
                     /translation="EEIVGEIADEYDREEPNIEPLPDGDYRVNARLGIDEVNELLDVE
                     LPSTEWDSIGGLVFNLIGDVPREGQEVEFMGLRLRAERVQGRRVGRIRIHRDAAEVGE
                     GAPAEARPS"
BASE COUNT          322 a         1079 c         1088 g          358 t
ORIGIN      
        1 ggggtggtgg cagctgcacc tccccgaccc ggagtggctg cggcgcgggc ggggccagcg
       61 cgtcgacgtg gtgtacgagc cggggccggg acgggtcgac ggctacgcca cctatggcct
      121 gcggtcgcgc tggaccgaca acatccccga cagcatcgcg caggtcatgg agctgatggc
      181 ggtgacgccg gaggcggcgg cgtcgctgtg gcgcttctgc ctggacctcg acctgaccac
      241 caccgtcgag ctgcggaacc ggcccctcga cgagccgctg cgctggtgcc tggcggaccc
      301 gcgccggctg cgcaccacgg cgctttccga cttcctgtgg ctgcgcgtcc tcgacctgcc
      361 cgccgccctg gccgcccggg gctacgccgc cgaagggcag ctcgtgctcg aggtcagcga
      421 cccgctcctg cccgacaacg acggccgctt ccggctcgac ggcgggccgg gcggcggccg
      481 gtgcgcgcgg acctccttcg cgccggacgt gatcctcgac gtggcggcgc tcggcgccgc
      541 ctacctcggc ggggtgcggt tctccaccct cggccgggcg ggccgggtcc gcgagggccg
      601 gccgggcgcg ctcgcccggg ccgacgcgat gttcgcgagc gacccggccc cctggtgctc
      661 cacggacttc tgagccggtc ggccacaccg gtccgatcgg ggctgccact cggggcggcg
      721 gcggtcccat ccctgcccgg ccgcccggac cgccacgcga tccggttcga cgggcaggtc
      781 gcagtggtca ccggcgccgg ccgcggcctc ggcgccgcct acgcacggct gctggcggcg
      841 cggggcgcgg cggtggtcgt gcacgacgcc ggcgtcgccg aggacggcag cggcttcgac
      901 tcgtcagtcg ccgacgcggt ggcggacgag atcgtcgagt ggggcgggac ggcgaccgcc
      961 tgccacgaga acctcgagtc cggggctggg tgccgccggg tcgtcgagct cgccgccgag
     1021 cagttcgggc gggtcgacgt gctggtccac aacgccggcc tggtggtgtt cgcgccggtc
     1081 gaggagaccg ccccggcgat cgcggagcgc tggccagagc tcaccgccgc cacgtgctag
     1141 aaccccaggc gggcgagctg cctggggtcg cgctgccact ccttggcgac cttcacgtgg
     1201 aggtcgaggt agacgcgggt gccgagcagg gcctccagct cggtccgggc ccgggtgccg
     1261 atctcgcgca gcaccgagcc ctgcctgccg agcacgatcg gcttctggct ggcccgctcg
     1321 acgaacaggg tggcccggac gacgagcagc gggtcgcggt cctccggctc ccgagccggg
     1381 cgcgccgtcc ggccctggcc gggccggagg ccgtccgggc ggggctcggg cgcctcggcc
     1441 ggcccgacct cgtcgaccag cacggcgacc gagtggggca gctcgtcgcg gaccagggcg
     1501 agcgccttct cgcggatcac ctcggcgatc aggtgctgct cgggctcgtc ggtgacgtgg
     1561 ccctcggggt acagcagcgg cccctcgggc aggtggtcga acagcaggcc ggcgagcacg
     1621 tcgagctgct cgccggtgcg ggccgagacg ggcacgacct cggcccagtc gccgagcgcc
     1681 gcggccgcct ccagcgcccg caccaggccg gcgcggggcg cggcgtccat cttgttcacc
     1741 acgcagacga ccggtgtgcc gaccctggcg agctcctcgg cgaggaagcg gtccccgcgg
     1801 cccaccccgg ccgcggcgtc gaccagcagg cagaccaggt cgacctcggc cagggtgccc
     1861 ctgacgaggt cgttcagccg ctggcccagg agcgtctggg gcttgtgcag gcccggggtg
     1921 tcgaccagca cggcctggcc gcgctcgtcg tggagcacgc cgcggaccac cgcccgcgtc
     1981 gtctgcggct tgtccgacat gatcgcgagc ttctggccga gcaggccgtt gagcagcgtg
     2041 gacttgccca cgttggggcg gccgacgagc gcgacgaggc ccgacttcac cggcaggcct
     2101 ccggggcggc gagctgctcg ggcccgaagc tctcgggcag cagtgctccg agggtggtca
     2161 ccagcagctg gtctcccgac tcgccggcgc agacgacctc gaggtcgagc gccgcgggga
     2221 actcgcgcag gacctgccgg cacgccccgc agggggtgac cggcgcctgc ccgtcgccgg
     2281 ccaccgcgat cgcctcgaag cgccggtagc cggctgccac ggcggcgacc acggcggccc
     2341 gctcggcgca gatgccgagg ccgtaggagg cgttctccac gttggcggcg gtcaccacgt
     2401 ccccgccctc ggcgagcagc gccgccccga cccggaaccc ggagtagggc gcgtaggcgc
     2461 gctcgcgggc gccgcgcgcg gcggcgacca gctcgccggt gggcaggccc gtcatgaggg
     2521 gcgggcctcg gccggggcgc cctcgcccac ctcggcggcg tcgcggtgga tgcggatccg
     2581 cccgacgcgg cggccctgga cccgctcggc ccgcagccgc aggcccatga actcgacctc
     2641 ctggccctcc ctgggcacgt cgccgatcag gttgaacacg agcccgccga tcgagtccca
     2701 ctcggtcgag ggcagctcga cgtcgagcag ctcgttcacc tcgtcgatcc cgagccgggc
     2761 gttgacccgg tagtcgccgt cggggagcgg ctcgatgttg ggctcctcgc ggtcgtactc
     2821 gtcggcgatc tcgccgacga tctcctc
//
LOCUS       DASPYY010000520         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2615701, whole genome shotgun sequence.
ACCESSION   DASPYY010000520 DASPYY010000000
VERSION     DASPYY010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615701"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VEP73_08260"
     CDS             complement(<1..1318)
                     /locus_tag="VEP73_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYT26454.1"
                     /translation="MPELSIETLPLLPLTSGVVLPGMVVTATLETPESQAAASAAADS
                     GGQLLLVPRVDGRYARFGTVAKVENAGELPSGQLAVVVRGLHRARIGTGVAGTGAATW
                     VEVERVPEQPATGRARELAREYRALVENILEARGAAQLAEMLRGITDPGQMADTAAYS
                     PDLPFERKVEVLETVDVEQRLEKVIAWARETLADVSLKDRIRKDVSEGMEKTQREYLL
                     RQQLAAIKKELGEGEGSEDVVEGYRAKVAESGMPEKVRAFVEREVDRLERTSEQSPEH
                     GWIRTWLDTMIELPWGRKSEDALDITDARRILDDDHTGLEDVKDRILEFLAVRKLRRE
                     RGLADTGRRGAGAILALVGPPGVGKTSLGESVARALGRSFVRVALGGIRDEAEIRGHR
                     RTYVGAQPGRIARAMKEAGTMNPVFLLDEVDKVGADWRGDPSSALLE"
     gene            complement(1429..>1785)
                     /locus_tag="VEP73_08265"
     CDS             complement(1429..>1785)
                     /locus_tag="VEP73_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26455.1"
                     /translation="TTERLLAVLGAAARTPDLTRFGLHAQGWGARTLAGHLAAVAEYR
                     DAKGRRVTLLRWRGRLPEAASGSPASGYARKLQAAEWGGTSSVWFRSGDVVWCLIGTT
                     DHQTLDAVADHLGADS"
BASE COUNT          214 a          650 c          662 g          259 t
ORIGIN      
        1 cctcgagcag cgccgaggac gggtccccgc gccagtcggc gccgaccttg tccacctcgt
       61 cgaggaggaa gaccgggttc atcgtgcccg cctccttcat ggcgcgggcg atgcggccgg
      121 gctgggcgcc gacgtaggtg cgccggtgcc cgcggatctc ggcctcgtcg cggatgccgc
      181 ccagggcgac ccgcacgaac gagcggccca gggcccgcgc gaccgactcc ccgagcgacg
      241 tcttgccgac ccccgggggc ccgacgagcg cgaggatcgc gcccgcgccc cgccggccgg
      301 tgtcggcgag gccgcgctcc ctgcggagct tgcgcacggc gaggaactcg aggatgcggt
      361 ccttcacgtc ctcgaggccg gtgtggtcgt cgtcgaggat gcgccgggcg tcggtgatgt
      421 cgagcgcgtc ctcggacttc ctgccccagg gcagctcgat catcgtgtcg agccaggtgc
      481 ggatccagcc gtgctcgggg ctctgctcgc tggtccgctc gagccggtcg acctcgcgct
      541 cgacgaacgc gcggaccttc tcgggcatgc cggactcggc caccttggcg cggtagccct
      601 cgaccacgtc ctccgagccc tcgccctcgc cgagctcctt cttgatggcg gcgagctgct
      661 ggcgcaggag gtactcccgc tgggtcttct ccatgccctc ggacacgtcc ttgcggatgc
      721 ggtccttcag gctcacgtcc gcgagcgtct cgcgggccca ggcgatgacc ttctcgaggc
      781 gctgctcgac gtcgacggtc tcgaggacct cgaccttgcg ctcgaacggg aggtcggggg
      841 agtaggcggc ggtgtcggcc atctgcccgg ggtcggtgat gccgcgcagc atctcggcca
      901 gctgggcggc cccgcgggcc tccaggatgt tctcgaccag ggcgcggtac tcgcgggcca
      961 gctcgcgggc gcgcccggtc gccggctgct cgggcacgcg ctcgacctcg acccaggtgg
     1021 ccgcgccggt gccggccacg ccggtgccga tgcgggcgcg gtgcaggccg cgcacgacca
     1081 cggcgagctg gccactcggc agctcgcccg cgttctccac cttggcgacg gtgccgaagc
     1141 gcgcgtagcg gccgtcgacc ctggggacga gcaggagctg tcccccggag tcggcggcgg
     1201 cgctggcggc cgcctgcgac tccggcgtct ccagggtcgc ggtgacgacc atgccgggca
     1261 gcaccacgcc gctggtcaac gggaggaggg gaagggtctc gatagagagc tcaggcattc
     1321 gttctccagg tcttcggggg tgcgtcgaca ggtgcaacgc cccgccgcac cggctcattc
     1381 ctggctatgg ccgatcattg aggacaagaa ctatctcggg agtcccgctc aggagtccgc
     1441 gccgagatgg tcggccacgg cgtcgagggt ctggtggtcg gtcgtgccga tcaggcacca
     1501 gacgacgtcc cccgaccgga accacacgct cgaggtcccg ccccactcgg cggcctgcag
     1561 cttccttgca tagccggagg ccggggaacc gctggccgcc tcgggcagcc ggccgcgcca
     1621 gcgcaggagg gtgacccgtc tgcccttcgc gtcgcggtac tcggccacgg ctgccaggtg
     1681 gccggcgagc gtgcgggcgc cccagccctg cgcgtgcagg ccgaaccgtg tgaggtcggg
     1741 ggtccgggcg gccgccccga ggaccgcgag caggcgttcg gtggt
//
LOCUS       DASPYY010000521         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_931039, whole genome shotgun sequence.
ACCESSION   DASPYY010000521 DASPYY010000000
VERSION     DASPYY010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931039"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VEP73_08270"
     CDS             complement(<1..955)
                     /locus_tag="VEP73_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HYT26456.1"
                     /translation="MAEPRKILLDEEEIPTAWRNIVPALPTRPGPYLHPRTREPVGPD
                     DLAPLFPMALIAQEVSTEPWIDIPGPVLDTYRLWRPTPLIRALDLERALGTPARIYYK
                     YEGGSPAGSHKPNTAVPQAYYNAEEGIRRLCTETGAGQWGSALAFACARFGLECKVYM
                     VRASYDGKPYRRSLMETWGASVVPSPSEETNAGRAVLAADPDSPGSLGIAISEAVEVA
                     AQSDDTHYALGSVLNHVLLHQTVIGLELQAQLAKAGIEPDVLVACVGGGSNFGGFTFP
                     ILADKLAGRSGVRVVAAEPTAAPTLTKGRYEYDLGDQAGMTP"
     gene            complement(1025..>1701)
                     /locus_tag="VEP73_08275"
     CDS             complement(1025..>1701)
                     /locus_tag="VEP73_08275"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYT26457.1"
                     /translation="VKRRSPSVGPIRPELDPAALAGAYERGGASAVSVLTEPVHFGGS
                     PADLEAVRAAVGLPVLRKDFVTSAYQVWEARAWGADAVLLIVAALDPGRLSGLLAEAA
                     AAGLDALVEVHTREEAGAAGAADAALVGVNARDLATLEVDSGRFARIRDALPSGATVV
                     AESGIATRADVEAAAAAGADAVLVGESLVRAGDPAALLAGLLGRPAPPARDTGPPRQA
                     RAARAR"
BASE COUNT          190 a          615 c          686 g          210 t
ORIGIN      
        1 gcggggtcat gcccgcctgg tcgccgaggt cgtactcgta gcggcccttg gtgagggtgg
       61 gcgccgcggt cggctcggcg gcgaccaccc ggacgccgga cctcccggcc agcttgtcgg
      121 cgaggatcgg gaaggtgaag ccgccgaagt tcgacccgcc gccgacgcag gcgacgagca
      181 cgtcgggctc gatgccggcc ttggcgagct gcgcctgcag ctcgaggccg atgaccgtct
      241 ggtggagcag gacgtggttc agcacgctgc cgagcgcgta gtgggtgtcg tcggactgcg
      301 cggcgacctc gaccgcctcg gagatggcga tgccgagcga gccgggcgag tccgggtcgg
      361 cggccagcac ggcgcggccg gcgttcgtct cctcggacgg cgacgggacc acggaggcgc
      421 cccaggtctc catgagcgag cgccggtagg gcttgccgtc gtagctggcc cggaccatgt
      481 agaccttgca ctcgagcccg aagcgggcgc acgcgaacgc cagcgcgctg ccccactgcc
      541 cggccccggt ctcggtgcag aggcgccgga tgccctcctc ggcgttgtag tacgcctggg
      601 ggacggcggt gttcggcttg tgcgagccgg ccggggagcc gccctcgtac ttgtagtaga
      661 tgcgggccgg ggtgccgagg gcccgctcga ggtcgagcgc gcggatcagc ggggtcggcc
      721 tccagaggcg gtaggtgtcg agcaccggcc cggggatgtc gatccagggc tcggtcgaga
      781 cctcctgcgc gatgagggcc atcgggaaca gcggggcgag gtcgtccggc ccgaccggct
      841 cgcgggtccg cgggtgcagg taggggccgg gcctggtcgg cagcgccggc acgatgttgc
      901 gccaggcggt cgggatctcc tcctcgtcca gcaggatctt cctcggctcg gccatcccgt
      961 ccctccttgc tctcggcgcg ccctggcgcg ccggtctcgg cgcgccctgc ggcgcgcacc
     1021 cgtctcagcg cgccctggcg gcgcgcgcct ggcgtggtgg cccggtgtcg cgggccggcg
     1081 gcgccggccg gccgagcagg ccggcgagca gggccgccgg gtcgccggcg cggaccagcg
     1141 actcccccac cagcaccgcg tcggcgccgg cggcggccgc cgcctccacg tccgcgcggg
     1201 tggcgatgcc gctctcggcg acgacggtgg cgccggacgg cagcgcgtcg cggatgcggg
     1261 cgaagcggcc gctgtcgacc tcgagggtgg cgaggtcgcg cgcgttgacg cccacgagcg
     1321 cggcgtcggc ggcgccggcg gcgcccgcct cctcgcgggt gtgcacctcg acgagtgcgt
     1381 cgaggccggc cgccgccgcc tcggccagaa gaccggacag gcgcccgggg tccagcgcgg
     1441 ccacgatcag gagcacggcg tcggcgcccc aggcgcgcgc ctcccagacc tggtaggcgc
     1501 tggtgacgaa gtccttgcgg agcacgggca ggccgacggc ggcccgcacg gcctccaggt
     1561 cggcggggct gccgccgaag tggaccggtt cggtgaggac gctgaccgcg ctcgccccgc
     1621 cgcgctcgta ggcgccggcc aacgcggccg ggtccagctc aggccggatc gggccgaccg
     1681 agggcgagcg ccgcttgacc t
//
LOCUS       DASPYY010000522         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_790641, whole genome shotgun sequence.
ACCESSION   DASPYY010000522 DASPYY010000000
VERSION     DASPYY010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790641"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..1249)
                     /locus_tag="VEP73_08280"
     CDS             complement(380..1249)
                     /locus_tag="VEP73_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYT26458.1"
                     /translation="MSGTARPAHSARHGRVRAAAMALAAVLAAVLAGCSGSGRPAGGP
                     APRGGIAPTTAPAFRVLVFTRTAGFRHASIPVGVAAIRRLGAEHGFAVDDTADAGRIG
                     GGGLAGYRVVVFLSTTGDLLDGAQRAALQRYVRGGGGWVGVHAAADAEYGWPWYGELV
                     GAFFRRHPAVQRAVVHVAPDATDAAGPLPARWERSDEWYDFRSNPRGRVRVLATVDES
                     TYSGGTMGHDHPIAWCRGLQGRNGRQGAPLGRGRSWYTAMGHTSESFQEPLFLAHLLA
                     GIRYAAGVSGARC"
     gene            1395..1751
                     /locus_tag="VEP73_08285"
     CDS             1395..1751
                     /locus_tag="VEP73_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26459.1"
                     /translation="MCTAVRIVVAAAERPRRLELRRASVAAEWEVVGEAADAEGAYQE
                     AVERRARFLVLDASAAGPRPEVLARRLRSTSPTAFVVGVGDVPGADTAVPADGLDGLR
                     DAMRSLLHGSGDHQHT"
     gene            complement(1799..2512)
                     /locus_tag="VEP73_08290"
     CDS             complement(1799..2512)
                     /locus_tag="VEP73_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26460.1"
                     /translation="MTPSDPRHLAEPRGTRPRAWRRVLLLVVGGLLAPAIAGAVALTV
                     WHDGSRRSLPAAGPPPATAAPSTTTAPGAPAGGEAPSRDRELIANPGFEASTAGWSPA
                     GEAVLERVRTAKAGRWSVKIVAGGPPRLAPGIAYTAVPVPDGEVGQEWEATVWARASR
                     PDTTVQVNLVEYVDGGRFAVDSAGTVLRGTSWHRIEVEHYLHRDRSRLAVEVVLPGLP
                     GGSAVLVDAAGMRPREGGS"
     gene            complement(2523..3020)
                     /locus_tag="VEP73_08295"
     CDS             complement(2523..3020)
                     /locus_tag="VEP73_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26461.1"
                     /translation="MRSLRMLLAAAAATLLLLPTAAAADPQIFLEDMSCSGVGALGEG
                     LPAGADLRLTLVSRDNGTTLASRTVTASGRGEFHDRIAAKLDEVLGVRLVVATPDGRQ
                     VGYADHQMLPGHAMCSLSAGGALPYTGAPRRALAVGVLGTGLVTLGALLVAAFAYRGR
                     RVPGR"
     gene            3283..4365
                     /locus_tag="VEP73_08300"
     CDS             3283..4365
                     /locus_tag="VEP73_08300"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT26462.1"
                     /translation="MAAGDRRDQRGGLPVPVLAGTVLAGGALAVQARVNGALSARIGS
                     AAVAALVAYGVASAALLPLVAGFRLSRRLPRGRRRGPVRWYWWLGGLAGAGVMAATAA
                     GVPRVGVALVSTCLVAGATAGGLVADWAGIGPGRPRPPSLRRLLGAGLAVGAVGVGTI
                     GQRAGHGQPLLVLLVFAAGLASAGQQTATGQLARVTGDARVAALVSSLVAAGGLAAVV
                     GSLAAAGRLPRLSWPASAWMYLGGLAVALYGTVAAAAVRRLGVLLVSLATVSGQLLAA
                     VALDAVVPAAGRLRATAVLGALLTLAAVGVVTTSHGAWSARAGADVRRDGAAGTAVNR
                     GRPGRWLARRSEGRRGPRRPHGRGRR"
     gene            complement(4277..4747)
                     /gene="nusB"
                     /locus_tag="VEP73_08305"
     CDS             complement(4277..4747)
                     /gene="nusB"
                     /locus_tag="VEP73_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYT26463.1"
                     /translation="MTDPLARPGARSAARRRALDILYEADVTQRPIATVLARHLHDPD
                     AERPDPYTVELVAGVDRLRDELDGRIGRAAEHWTVERMPLVDRNLLRLAAYELVARPE
                     VPTAVVLNEAIDLAKLLSTADSGRFVNGVLGRVAGEVRGGPRNGGPATGPAVPG"
BASE COUNT          539 a         1789 c         1879 g          578 t
ORIGIN      
        1 tcgccgacgt caccgccgcg gccgagcgcg gcatccaccg catccggggc accccgcacc
       61 tggcctactc gttcctgcgc cactgcggcc tgtccctctg gtgagtgtga tcatgtcgct
      121 ggaatgcgcg aacttctata atgagccggt caggtagccg gaatgacctg cttgcggcca
      181 ctgcagagga ctcctgaccc gcatcctctc gagcgccgtg atgggcggtg tccagcttga
      241 tctccacgat cgccatgtat tgcccctgga gcggcagcaa ctttgtgacc gtcgaaccaa
      301 actcgatgac ggctgatgcc gcgcatgtta gatgaccgcg acgcggtgca agtcgagcag
      361 gtgcttgcgc ttcccgaggt cagcaacgag cgccgctgac gcccgcggcg tagcggatgc
      421 cggcgagcag atgcgccagg aacagcggtt cctggaagct ctccgaggtg tggcccatgg
      481 ccgtgtacca gctccggccg cgacccagcg gtgcgccctg ccggccattc ctgccttgga
      541 gcccgcggca ccaggcgatc gggtggtcgt ggcccatcgt cccgcccgag taggtcgact
      601 cgtcgacggt ggcgagcacc cggacgcgcc cgcgagggtt ggaccggaag tcgtaccact
      661 cgtcgctgcg ctcccagcgg gccggcaggg ggccggccgc gtcggtggcg tccggcgcga
      721 cgtggacgac ggcgcgctgg acggcggggt ggcggcggaa gaaggcgccg accagctcgc
      781 cgtaccaggg ccagccgtac tcggcgtcgg ccgccgcgtg gacgccgacc cagccaccgc
      841 cgccgcggac gtagcgctgc agggcggccc gctgggcgcc gtcgagcagg tcgccggttg
      901 tggagaggaa cacgacgacc cggtagccgg cgagtccccc gccgccgata cggccggcgt
      961 cggcggtgtc gtcgaccgcg aagccgtgct cggcgccgag gcgccggatg gcggcgacgc
     1021 cgaccgggat cgaggcgtgc cggaagccgg ccgtcctcgt gaagaccagc acccggaagg
     1081 caggcgcggt ggtgggcgcg atcccgcccc ggggcgcggg ccctccggcg ggacgaccgc
     1141 tcccggagca gccggcgagc accgcggcga ggaccgccgc gagcgccatc gcggcggccc
     1201 gcacccggcc gtgacgtgcg gagtgtgcag ggcgtgccgt cccgctcacc tcctcgaccc
     1261 cgtgtcaggc tgcgcccagg gatccggccg ggccgctcgg ccccggcggc taggatagcc
     1321 acccggcggg cggccggccg ccccacgcgg ccacggcccg ccggcgaccg gacccgagag
     1381 ccaggagccc gcccgtgtgc accgccgtcc gcatcgtcgt cgccgccgcc gagcggccgc
     1441 ggcgcctcga gctgcgccgg gcctcggtgg ccgcggagtg ggaggtcgtc ggcgaggcgg
     1501 ccgacgccga gggcgcctac caggaggcgg tcgagcggcg ggcccgcttc ctcgtcctgg
     1561 acgcgagcgc ggccggcccc cggccggagg tgctcgcccg ccggctccgg tcgaccagcc
     1621 cgaccgcgtt cgtcgtcggt gtcggcgacg tccccggcgc cgacacggcg gtcccggccg
     1681 acgggctgga cggcctgcgg gacgccatgc ggtccctgct gcacggctcg ggcgaccacc
     1741 agcacacctg agcgccaccc tgcggccgcg cggccacgat gcggcccggc cggggcggtc
     1801 aggacccgcc ctcgcggggc cgcatgccgg ccgcgtcgac cagcacagcc gacccgccgg
     1861 ggaggccggg gaggaccacc tccacggcga gccgcgagcg gtcccggtgc aggtagtgct
     1921 ccacctcgat ccggtgccaa ctggtgccgc gcaggacggt gccggccgag tcgaccgcga
     1981 agcgcccacc gtcgacatat tcgaccaggt tcacctggac ggtcgtgtcc ggccggctcg
     2041 cgcgggccca caccgtcgcc tcccattcct ggccgacctc gccgtccggg accggcacgg
     2101 ccgtgtacgc gatgccgggg gcgagccgcg gcggcccccc ggcgacgatc ttcaccgacc
     2161 acctgccggc cttcgcggtc cggacgcgct ccaggaccgc ctcaccggcc gggctccagc
     2221 ccgccgtgct ggcctcgaag ccggggttgg cgatcagctc ccggtcgcgg gaaggcgcct
     2281 cgccgccggc cggtgctccg ggggcggtcg tcgtcgacgg cgccgccgtg gccggcggcg
     2341 ggccggccgc cgggagcgac cgccgggagc cgtcgtgcca gaccgtcagg gcgaccgccc
     2401 cggcgatcgc cggggcgagc aggccgccga cgacgagcag gagcacccgg cgccacgccc
     2461 ggggccgggt tccccgtggc tcggccaggt gcctcgggtc ggacggggtc atgagcggtc
     2521 cgctaccggc cggggacgcg gcgccctcgg taggcgaagg ccgcgaccag gagggctccc
     2581 agcgtgacca ggccggtgcc gagcaccccg acggccaggg cgcggcgcgg cgcccccgtg
     2641 tagggcaggg cgccgccggc ggacaggctg cacatggcgt ggccgggcag catctggtgg
     2701 tcggcgtagc cgacctgcct gccgtccggc gtcgcgacca ccagccggac gccgagcacc
     2761 tcgtcgagct tggccgcgat gcggtcgtgg aattcgcccc tgccagaggc agtcaccgtc
     2821 ctgctggcga gcgtcgtgcc gttgtccctg ctcaccagcg tgaggcgcag atccgcccct
     2881 gcgggcaggc cctcgccgag ggcgccgacg ccgctgcagc tcatgtcctc gaggaagatc
     2941 tgcgggtcgg cggcggcggc ggtcgggagc agcagcagcg tggccgccgc cgcggccagc
     3001 agcatcctga gcgaccgcac ggccccgccc tcccctctac ccgacgcccg ggctcccgac
     3061 gcccggggat cacccggacg ttgaggaatc aatcaagcac gggcgcaact attggcgaga
     3121 cgcggccgta gagcaactgt cttgaagaca accagcctgt cgaacgacct ggcctggtca
     3181 cgccgccagc ccgcggcgcc ggccgtgtcg cgtggttgtc gtaccgtcac cgcgggatcc
     3241 gggagcctgg cggcccgggc gagcacggga gggcaggggc acgtggcagc cggcgaccgg
     3301 cgtgaccagc gcggcggcct gcccgtgccc gtgctggccg gcacggtcct ggccggcggg
     3361 gcgctcgccg tgcaggcgcg ggtcaacggt gcgctcagcg cccggatcgg gtcggcggcc
     3421 gtggcggcgc tggtggccta cggcgtcgcc agcgcggcgc tgctgcccct cgtcgcaggc
     3481 ttccggctga gccgtcgcct gccgcgtggg cgccgccggg gaccggtccg ctggtactgg
     3541 tggctcggcg ggctggctgg cgccggggtg atggcggcca cggccgccgg cgtgccgcgc
     3601 gtcggggtcg cgctggtcag cacctgcctg gtcgctggtg cgaccgcagg cggcctggtc
     3661 gcggactggg cggggatcgg cccgggacgg ccgcgcccgc cctcgctccg ccggctgctg
     3721 ggtgcgggcc tcgcggtcgg tgcggtgggc gtcggtacga tcggacaacg cgcggggcac
     3781 ggccagccgt tgctggtcct gctcgtcttc gccgccggac tcgcctccgc cggccagcag
     3841 acggccaccg gccagctggc ccgggtcacc ggcgacgcgc gcgtggcggc gctggtgtcc
     3901 tcgctggtgg ccgccggggg ccttgccgcc gtggtggggt cgctcgcggc ggccggccgg
     3961 ttgccgcggc tgtcctggcc ggccagcgca tggatgtacc tgggcggtct cgccgtcgcg
     4021 ctgtacggga cggtggccgc ggcggcggtc cgccgcctgg gcgtgctgct cgtcagcctg
     4081 gcgaccgtca gcggtcagct gctggcggcc gtcgcgctcg acgcggtggt ccccgcagcg
     4141 ggccggctcc gcgccacggc cgtcctcggg gcgctgctca cgctcgccgc cgtcggggtg
     4201 gtgaccacct cgcatggcgc ctggtcggcg agagcggggg cggacgtcag gcgggacggc
     4261 gcggcgggga cggcggtcaa ccggggacgg ccgggccggt ggctggcccg ccgttccgag
     4321 ggccgccgcg gacctcgccg gccacacggc cgaggacgcc gttgacgaag cgcccggagt
     4381 cggcggtcga cagcagcttc gcgaggtcga tggcctcgtt gagcacgacc gcggtgggga
     4441 cctccggccg ggccacgagc tcgtaggcgg cgagccggag gaggttccgg tcgaccagcg
     4501 gcatccgctc gaccgtccag tgctcggcgg cgcgcccgat gcgcccgtcg agctcgtcgc
     4561 ggagccggtc caccccggcg accagctcga ccgtgtacgg gtcgggacgc tcggcgtcgg
     4621 ggtcgtgaag gtggcgggcg agcacggtgg cgatgggccg ctgggtcacg tccgcctcgt
     4681 agaggatgtc gagggctcgc cggcgggccg ccgaccgggc tcccggccgg gcgagcggat
     4741 cggtcatggc gtcaccgggg tcgcaccggg gcgctcgtcg gacca
//
LOCUS       DASPYY010000523         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1634186, whole genome shotgun sequence.
ACCESSION   DASPYY010000523 DASPYY010000000
VERSION     DASPYY010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634186"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VEP73_08310"
     CDS             <1..458
                     /locus_tag="VEP73_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYT26464.1"
                     /translation="LVTWIAAAPGGGLPRDHARRVAAGVAATLDVPAVRLLAPAPGPD
                     LGRARRAGAAASRPAPRPRRRLAGGRVLVVDDLATTGATLAGAAAALRRAGAGHVEGA
                     VVGAAGTAFGPVPAVGGATLPGMSAALGQITVCAAQCGVLCTCAPVDPV"
     gene            626..1180
                     /gene="raiA"
                     /locus_tag="VEP73_08315"
     CDS             626..1180
                     /gene="raiA"
                     /locus_tag="VEP73_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYT26465.1"
                     /translation="MQVTVKGKNTAVPDELRAQAERKLAKVQRFDDRILAMDVEFSEE
                     RNPRVADAHRVEVTLTTKSRLVRAQATAADPTTAIDRVIDRLKSQIKKLKDRRVDRTR
                     HAAGTKALVQLADDGEAGPEPASPPIARFKRVELKPMAPEEAIERMDLLGHDFFLFTN
                     AETELANVVYRRRSGDYGLIEPET"
     gene            1223..1954
                     /locus_tag="VEP73_08320"
     CDS             1223..1954
                     /locus_tag="VEP73_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26466.1"
                     /translation="MTTQEEARGESVADDRQARPRGAGDAIRVLIVDDHALFRRGLEM
                     VLEQEDDVDVAGEAGDGAEAIEKAQELMPDVVLMDVRMPRRGGIEATQAIKDLIPHVK
                     ILMLTISDEEADLYDAIKAGANGYLLKEISIEEVANAIRSVAQGQSLISPSMASKLLN
                     EFATMVKRADEKQQLPTPRLTDREMEVLKLVAKGHNNRDIAKELYISENTVKNHIRNI
                     LEKLHLHSRMEAVVYAVREKLLEIK"
     gene            1982..2950
                     /locus_tag="VEP73_08325"
     CDS             1982..2950
                     /locus_tag="VEP73_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT26467.1"
                     /translation="MPAALALDVGGTKLAVGVVDARGRVLGRSRMPTPAGDDPEPLYQ
                     ALLACAAAAMRDAGAVAGGLAGVGVGCGGPMRWPDGAVSPLNIPAWRDFPLRRRLARD
                     LGTGAVLVHDDAVAIAAGEHWRGAGTGAANLLGLTVSTGVGGGLVLDGRLYHGTSGNG
                     GHIGHTVVEPDGPPCACGGRGCLEAVASGPRTVARALADGWRPGGDGARGAAADGVAL
                     AAAAAGGDPVAGAAVARAARAVGLAVASCANLLDLEVVAIGGGFSRSGRAFWDPLRDA
                     VVTHARLPFAAACRVVPARLGADAGLLGAAAFILRPDRYGWAAPRD"
BASE COUNT          440 a         1224 c         1088 g          380 t
ORIGIN      
        1 acctcgtgac ctggatcgcc gccgcgccag gcggcggcct ccccagggat cacgcccggc
       61 gcgtcgccgc cggcgtcgcc gccaccctcg acgtgcccgc ggtccggctg ctcgccccgg
      121 ccccaggacc cgacctcggc cgggcccggc gcgccggcgc ggccgcgtcc cggccggcgc
      181 cgcgtccccg tcggcgcctc gcgggcgggc gcgtcctcgt cgtcgacgac ctggccacca
      241 ccggcgccac cctggcaggg gcggccgcag ccctccgccg cgccggcgcc ggccacgtcg
      301 agggtgccgt ggtcggggcc gccgggaccg ccttcggccc ggtcccggcc gtcggtgggg
      361 ccaccctgcc cgggatgtcc gccgcgctcg gccagattac tgtttgcgca gctcaatgcg
      421 gcgtattgtg tacttgtgcc cccgttgacc cggtgtagaa tccggccaat acgacccggt
      481 acctgtcaag caggcccgtt cggatgccga ccgatccagg caggccgacg tcgccacccg
      541 tcgtgcaacg agatcgagca ggaccggtcg agcgtgtgtc gcccgccccc gaggccattg
      601 gccgacgacc aggatgggag ctgccatgca ggtgacggtg aagggcaaga acaccgccgt
      661 gcccgacgag ctgcgggccc aggccgagcg caagctcgcc aaggtccagc ggttcgacga
      721 ccggatcctg gccatggacg tcgagttctc cgaggagcgc aacccccggg tcgccgacgc
      781 ccaccgcgtc gaggtgaccc tcaccaccaa gtcgcggctc gtccgcgccc aggccaccgc
      841 ggccgacccg accaccgcga tcgaccgggt gatcgaccgg ctcaagagcc agatcaagaa
      901 gctcaaggac cgccgggtgg acaggacccg gcacgccgcg gggaccaagg cgctcgtcca
      961 gctcgccgac gacggcgagg ccggcccgga gccggccagc ccgccgatcg cccgtttcaa
     1021 gcgggtcgag ctcaagccga tggcgcccga ggaggcgatc gagcgcatgg acctgctcgg
     1081 tcacgacttc ttcctgttca cgaacgccga gactgaactg gccaacgtcg tgtaccgtcg
     1141 gcgtagcggc gattacgggc tcatcgaacc agagacctag cgagccgtac cggctcgtcc
     1201 gaacgtcgag gcgccggaca gcatgacgac acaagaggaa gcgagagggg agagcgtggc
     1261 ggacgaccgg caggcgcgac cgcggggcgc aggagacgcc atccgcgtgc tgatcgtgga
     1321 tgaccacgcg ttgttccgcc gcgggctcga gatggtcctc gagcaggagg acgacgtcga
     1381 cgtggccggg gaggccggcg acggtgccga ggcgatcgag aaggcccagg agctgatgcc
     1441 cgacgtcgtg ctgatggacg tccgcatgcc ccgccgcggc ggcatcgagg ccacccaggc
     1501 catcaaggac ctcatcccgc acgtgaagat cctgatgctg accatctccg acgaggaggc
     1561 cgacctctac gacgccatca aggcgggcgc caacggctac ctcctcaagg agatctcgat
     1621 cgaggaggtc gccaacgcga tccgctcggt cgcccagggc cagtccctca tctccccgtc
     1681 gatggcgtcc aagctcctca acgagttcgc gaccatggtc aagcgcgccg acgagaagca
     1741 gcagctcccc accccgcggc tcaccgaccg cgagatggag gtcctgaagc tcgtcgccaa
     1801 gggccacaac aaccgcgaca tcgccaagga gctctacatc tccgagaaca ccgtgaagaa
     1861 ccacatccgc aacatcctcg agaagctcca cctgcactcg aggatggagg cggtcgtcta
     1921 cgcggtgcgg gagaagctgc tcgagatcaa gtagcgacgt gcgcgcctcc ccggtgccct
     1981 agtgccggcc gccctcgccc tggacgtggg cggcaccaag ctcgccgtcg gcgtggtgga
     2041 cgcacgtggc cgcgtactcg gccggagccg catgcccacc ccggccggcg acgaccccga
     2101 gccgctctac caggcgctgc tggcctgcgc cgccgctgcc atgcgcgacg ccggcgccgt
     2161 cgcgggcggc cttgccggcg tcggcgtcgg ctgcggcggt ccgatgcgct ggcccgacgg
     2221 cgccgtctcc ccgctcaaca tcccggcctg gcgcgacttc cccctgcggc ggcgcctcgc
     2281 gagggacctc ggcaccggcg cggtcctggt ccacgacgac gccgtcgcga tcgcggccgg
     2341 cgagcactgg aggggcgccg gcaccggcgc cgccaacctg ctcggcctga ccgtctccac
     2401 gggggtcggc ggcggcctcg tcctcgacgg ccgtctctac cacggcacca gcggcaacgg
     2461 cggccacatc ggccacaccg tcgtcgagcc cgacggcccc ccgtgtgcct gcggtggccg
     2521 cggctgcctg gaggcggtcg cgtccgggcc gcgcacggtc gcccgcgccc tcgccgacgg
     2581 atggcgccca ggaggcgacg gcgcgcgcgg cgcggccgcc gacggggttg cgctggccgc
     2641 ggccgccgcc ggcggcgacc cggtcgccgg cgccgccgtg gcccgcgccg cgcgcgccgt
     2701 cggcctcgcc gtcgcctcct gcgccaacct cctcgacctg gaggtcgtcg ccatcggcgg
     2761 cggcttctcc cggtccggcc gggcgttctg ggatcccctt cgggacgccg tcgtgaccca
     2821 tgcacgcctg ccgttcgccg cggcctgccg cgtcgtgccc gcccgcctcg gcgccgacgc
     2881 cggcctgctc ggcgcggccg cgttcatcct ccggcccgac cgctacggct gggccgcgcc
     2941 ccgcgactga cgcccccgcc cggcgaacag gccagccccg ggatccacat gaccggcccg
     3001 cccgagacca cgccgaccgc cggccggtag actggttcga tccgcatgtg atccgcatgt
     3061 gctcaacgcg ccggccggct cgcgcgaggc ccggccgcga ggaggcgtcc aagtcgtggt
     3121 gctgtccaag gt
//
LOCUS       DASPYY010000524         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_229656, whole genome shotgun sequence.
ACCESSION   DASPYY010000524 DASPYY010000000
VERSION     DASPYY010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229656"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1096)
                     /locus_tag="VEP73_08330"
     CDS             complement(83..1096)
                     /locus_tag="VEP73_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYT26468.1"
                     /translation="MAGPAGPAGSTVPHPRDLGLAEARALVRRAVDKAEQLGLRGAVA
                     VVGASGTLLTASRMDAGGPGGMARARSKAWISATQQIPSSEHLHRMTTIAPPVAAGFA
                     QASPEALFPGAGGIPVSVGGVVVAGIAASGATVSPFFPEGVDPRVVSADGRPANPEDL
                     LIAYALGVPYTGQHGDDQKRWEQRFGDLAVDPADSLGMAPAPPAGRQAQLDWARGLCD
                     AAMDAATRRGVRVAVAVVDRGGDPVQQDLMDSAPAGGVAIAQAVAGAAALFDCCSGNL
                     GARFGQPDGGPALAALVVPPVLGIPGGLPVRDGGQVVAGFGVGGGDPDACAEIARIAL
                     DAS"
     gene            complement(1099..1893)
                     /locus_tag="VEP73_08335"
     CDS             complement(1099..1893)
                     /locus_tag="VEP73_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005476108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT26469.1"
                     /translation="MPAAPVPVPRRFEGRSVVVTGAGTGFGAEIAVRAAQEGARAVGV
                     HYRSSVEGAKRTADRVTAEGSTPVLLQADIVDWEQIKAMADAAFERLGGVDVLVNNVG
                     DVAREQMSWRDITESSIDHVLAVDIKGTMLCVHEFGARMLEAGHGAIVNIGSTVIVRG
                     SARAPQYAAAKYGILGLTKSYAHAFAPAVRVNVFAPGFIETEATLGRKDWQSGRGDQL
                     RSLTPMGRIPGPAELAGTALFLATDDASHMTGGYMVADGGYNMVGA"
BASE COUNT          247 a          745 c          724 g          237 t
ORIGIN      
        1 aggtgcgcgg cgtacaggct cccgatcgcg ccgcagccga tgaccgcgac gcggacggct
       61 gggctggtat ggtccgggcc ggtcacgacg cgtcgagcgc tatccgggcg atctccgcgc
      121 aggcgtcggg atcgccgccg ccgacgccga aaccggccac cacctgcccg ccgtcgcgga
      181 ccggcaggcc gccggggatg ccgagcaccg gcggcaccac gagcgccgcc agcgccgggc
      241 ccccgtccgg ctggccgaac cgggcgccga ggttgccgct gcaacaatcg aatagggccg
      301 ccgcgcccgc caccgcctgc gcgatggcga ccccgccggc cggggcgctg tccatcaggt
      361 cctgctggac cgggtcgccg ccgcggtcca ccacggccac cgccacccgg acgccgcggc
      421 gggtcgccgc gtccatggcg gcatcgcaga gtccccgggc ccagtcgagc tgggcctggc
      481 ggcccgccgg gggagcgggg gccatgccga ggctgtcggc ggggtcgacg gccaggtcac
      541 cgaagcgctg ctcccagcgc ttctggtcgt ccccgtgctg tcccgtgtac gggacgccga
      601 gggcgtaggc gatcagcagg tcctcggggt tggccggcct gccgtccgcg ctcaccacgc
      661 gcgggtcgac accctcgggg aagaacgggc tcacggtcgc gcccgaggcc gcgatgccgg
      721 ccacgaccac gccgccgacg ctgaccggta tcccgcccgc accggggaac agcgcctccg
      781 gcgacgcctg ggcgaacccg gcggcgaccg gcggggcgat cgtcgtcatc cggtgcaggt
      841 gctccgagct cgggatctgc tgcgtcgccg agatccaggc cttggacctg gcacgcgcca
      901 taccgcccgg cccgcccgcg tccatccggg acgcggtgag cagggtcccc gacgccccga
      961 ccacggccac cgccccgcgc agcccgagct gctcggcctt gtccacggcg cgccgcacca
     1021 gcgcgcgggc ctcggccagg ccgagatccc gcgggtgcgg aaccgtagac cccgcaggcc
     1081 cggcaggccc ggccatggct aagcgcccac catgttgtag ccgccgtcgg cgaccatgta
     1141 cccgccggtc atatgcgagg cgtcgtcggt ggccaggaac agggcggtgc ccgcgagctc
     1201 ggccggcccg gggatccgcc ccatgggggt gagcgaccgc agctggtcgc cccggccgga
     1261 ctgccagtcc ttgcggccca gggtggcctc ggtctcgatg aacccggggg cgaagacgtt
     1321 gacccggacc gcgggcgcga aggcgtgcgc gtaggacttg gtcaggccca ggatcccgta
     1381 cttcgccgcg gcgtactgcg gcgcccgcgc gctgccgcgc acgatgacgg tcgacccgat
     1441 gttcacgatg gccccgtggc ccgcctccag catccgcgcg ccgaactcgt gcacgcacag
     1501 catcgtgccc ttgatgtcga ccgcgaggac gtggtcgatc gaggactcgg tgatgtcgcg
     1561 ccaggacatc tgctcgcggg cgacgtcgcc gacgttgttc accagcacgt ccacgccgcc
     1621 gaggcgctcg aaggcggcgt cggccatggc cttgatctgc tcccagtcga cgatgtcggc
     1681 ctggagcagc accggggtgc tgccctccgc cgtgacccgg tcggccgtgc gcttggcgcc
     1741 ctctacggag ctgcggtaat ggacgccgac ggcgcgggct ccctcctggg cggcgcggac
     1801 cgcgatctcg gcgccgaagc cggtgccggc gccggtgacg acgacggacc gcccctcgaa
     1861 gcggcgcggt acgggaacgg gtgctgcggg cacggggtgt ccttcctgag taggtggctg
     1921 gggcggcccg tcctgggcga ctcagaccag gac
//
LOCUS       DASPYY010000525         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_791075, whole genome shotgun sequence.
ACCESSION   DASPYY010000525 DASPYY010000000
VERSION     DASPYY010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791075"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VEP73_08340"
     CDS             complement(<1..771)
                     /locus_tag="VEP73_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYT26470.1"
                     /translation="MEPDRGEGRAAPSAEDAARRVDELRARLNDASYRYHVLSDPDIS
                     DAEYDEMLQELIRLEGEFPDLVTADSPTQTVGAPPSAVFAPVRHRVPMLSLDNAFAAE
                     ELDAWGKRTERAVGQVDAYVCELKIDGIAVSLQYDRGRLVRAATRGDGVVGDDITRNA
                     RTIAGVPRRLEVDDPPEFFEVRGEIYFPTAAFERLNEQQAAEGRQVFANPRNAASGAL
                     RQKDPHVTAARPLALLCHSSGIAEGLRFAAHSEFLDWCQ"
     gene            843..3083
                     /locus_tag="VEP73_08345"
     CDS             843..3083
                     /locus_tag="VEP73_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYT26471.1"
                     /translation="MFVALARFVLRRRWLVVAAWAAVLAAGFGYGSQVFGRLASNSGL
                     APDAESVMTSERLQRLARTGPEILVLVDGRRVDDPALRASVASAAREIERVPGVARVT
                     DGYRAPSRGLMARDGGATLLRVELAESAPREERDRAVAAVRERASGIDAPRVLVGGQV
                     TANREFQRQAQRDLEKGELYALPVMLVLLLVIFGGRGAARRLERAGFRSRVAGAFGRV
                     LPVVLSAGMPLLLAAVAVGGALLLLLALSHATDISSYSVNVVTMLGLGLAVDYSLLVI
                     SRFREERGADLELAGALERTMASAGRTVAFSGLTVAVSLSGLLVFAEPFLRSLAWGGI
                     GVVLAAMLAAITLVPALLAVWGGRIHPPRQGRPDQGLFYRQSRLVQRFALVIVPVVAA
                     CLLVAALPFRAAELENSGLEALPRSSESRQLFQTVRARFPGAGTDPVTVVVDASPRAP
                     ELLGYLRRIQGLPDVDSAQLRSLPLPVLTVVDVVPAGSSQGPAARRLVERVRALDRPA
                     AAGVTGSAAFLVDYRDSLARRLPFAFGLIGLTTLVLLFLMTGSVVVPLKAVVMNVLSL
                     GASFGALVWVFQEGHLSGLLGFDAPGAVDISVPVLIFIFAFGLSMDYEVFLISRIKEA
                     WDETGDNDRAVALGLQRTGRIVTSAALLIVVVFLGFALGELLTIKEIGLGMAIAVIVD
                     ATVVRTLLVPATMKLVGRWNWWAPGPLRRLHDRFGVAEHAPAPAPAPAGAPADAGRKE
                     LGAPVR"
BASE COUNT          337 a         1213 c         1214 g          484 t
ORIGIN      
        1 ctggcaccag tccaggaact cggagtgggc ggcgaagcgc agcccctcgg cgatcccgga
       61 cgagtggcac agcagcgcga gcgggcgggc ggcggtgacg tgcgggtcct tctggcgcag
      121 cgcgccagag gcggcgttgc gggggttggc gaacacctgc ctgccctcgg cggcctgctg
      181 ctcgttgagc cgctcgaagg cggcggtcgg gaagtagatc tcgccgcgga cctcgaagaa
      241 ctcaggcggg tcgtcgacct cgagccgccg cgggacgccg gcgatggtcc gcgcgttccg
      301 cgtgatgtcg tcgccgacga cgccgtcgcc cctggtcgcc gcgcggacca gccggccgcg
      361 gtcgtactgg agggagacgg cgatgccgtc gatcttgagc tcgcagacgt aggcgtcgac
      421 ctggccgacg gcgcgctcgg tccgtttgcc ccaggcgtcg agctcctcgg ccgcgaacgc
      481 gttgtcgagg gagagcatgg ggacacggtg cctgaccggg gcgaacacgg ccgacggggg
      541 cgcgccgacc gtctgggtgg gcgagtcggc ggtgacgagg tccgggaact cgccctcgag
      601 ccggatgagc tcctgcagca tctcgtcgta ctcggcgtcc gagatgtcgg ggtcggagag
      661 cacgtggtag cggtaggacg cgtcgttcag cctggcccgc agctcgtcga cccgccgcgc
      721 ggcgtcctcc gccgacggcg ccgcgcggcc ctccccccgg tctggttcca tggcgtaagt
      781 atcccgcacc ctgtggaagg atcccaggaa gcaccacgcc ctacgctccg gaggtcgccc
      841 gcgtgttcgt cgctctcgcc cggttcgtcc tgcgccggcg ctggctcgtc gtcgccgcct
      901 gggcggcggt gctggccgcc ggcttcgggt acgggtcgca ggtgttcggg cggctcgcca
      961 gcaacagcgg cctcgccccg gacgccgagt cggtcatgac gtccgagcgc ctgcagcggc
     1021 tggcccgcac cggcccggag atcctcgtgc tcgtcgacgg ccgccgggtc gacgaccccg
     1081 ccctgcgcgc gagcgtcgcc tcggcggcca gggagatcga gcgcgtgccc ggcgtggcca
     1141 gggtgaccga cgggtaccgg gcgccctcgc gcgggctcat ggccagggac ggaggggcca
     1201 cgctgctccg cgtcgagctc gccgagagcg cgccgcgcga ggagcgcgac cgtgcggtcg
     1261 ccgcggtccg cgagcgggcc tcgggcatcg acgcccccag ggtgctggtc ggcggccagg
     1321 tcaccgcgaa ccgggagttc cagcgccagg cccagcggga cctcgagaag ggcgagctgt
     1381 acgcgctgcc ggtcatgctc gtcctgctgc tggtcatctt cggcggccgc ggcgccgcgc
     1441 gccggctcga gcgggccggg ttccggtccc gggtcgcggg cgcattcggc cgggtgctcc
     1501 cggtcgtgct ctccgccggg atgccgctgc tgctggcggc cgtcgcggtc ggcggggcgc
     1561 tgctcctcct gctcgcgctc agccacgcca ccgacatctc gtcgtactcc gtcaacgtgg
     1621 tcaccatgct gggcctcggc ctggccgtcg actactccct gctcgtcatc agccggttcc
     1681 gggaggagcg gggggccgac ctggagctcg ccggcgccct cgagcgcacc atggccagcg
     1741 cgggccggac ggtcgccttc tccggcctca cggtggcggt ctccctctcc gggctgctcg
     1801 tcttcgccga gcccttcctg cgctcgctcg cctggggcgg catcggggtg gtgctcgccg
     1861 ccatgctcgc cgcgatcacg ctggtgcccg ccctgctggc ggtctggggc ggccgcatcc
     1921 acccacccag gcagggccgg cccgaccagg gcctcttcta ccgccagtcc cggctcgtgc
     1981 agcgcttcgc gctcgtgatc gtgcccgtcg tggcggcctg cctcctcgtc gccgcgctgc
     2041 ccttccgcgc cgcggagctc gagaactcgg gcctcgaggc cctgccccgc tcctcggagt
     2101 cgcgccagct cttccagacg gtgcgggccc ggttccctgg tgccggcacc gacccggtca
     2161 ccgtcgtggt cgacgcgagc ccgcgggcgc ccgagctgct cggctaccta cgccggatcc
     2221 agggactacc cgacgtcgac tcggcccagc tgcggtcgct gccgctcccc gtcctcacgg
     2281 tcgtcgacgt cgtccccgcc gggtcgagcc agggaccggc ggcccgccgg ctggtcgagc
     2341 gcgtccgggc gctcgaccga ccggccgccg ccggggtgac cgggtcggcc gcgttcctgg
     2401 tcgactaccg cgactcgctg gcccggcgcc tgccgttcgc gttcgggctg atcggcctca
     2461 ccaccctcgt cctgctgttc ctgatgaccg ggtcggtcgt cgtgccgctc aaggcggtgg
     2521 tgatgaacgt cctcagcctg ggcgcgagct tcggcgccct cgtgtgggtc ttccaggagg
     2581 ggcacctgtc cggcctgctc ggcttcgacg cccccggcgc ggtcgacatc tcggtccccg
     2641 tgctgatctt catcttcgcc ttcggcctct cgatggacta cgaggtgttc ctcatctccc
     2701 gcatcaagga ggcgtgggac gagaccggcg acaacgaccg cgcggtcgcc ctcgggctgc
     2761 agcgcaccgg ccggatcgtg acctcggccg ccctcctgat cgtggtcgtg ttcctgggct
     2821 tcgcgctggg cgagctcctg accatcaagg agatcgggct cggcatggcg atcgcggtga
     2881 tcgtcgacgc caccgtggtc cgcacgctgc tcgtgcccgc gacgatgaag ctcgtgggcc
     2941 gctggaactg gtgggccccc gggccgctgc gccgcctgca cgaccgcttc ggcgtggccg
     3001 agcacgcgcc ggcgccggcg ccggccccgg ccggcgcgcc ggcggatgcc ggccggaagg
     3061 agctgggcgc cccggtacgg tgaggcgcct ggcgggcgcc gggcgcccct ggcgggggag
     3121 ccgggccggg aacccgagcc tggggggccg acggggagcg ggagcggtac gctggtgcga
     3181 tgccagccag cgcggtcgag ctcgtggtca ccgacctcga cgggacgctg tccgacgcca
     3241 ccgagcgc
//
LOCUS       DASPYY010000526         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1774504, whole genome shotgun sequence.
ACCESSION   DASPYY010000526 DASPYY010000000
VERSION     DASPYY010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774504"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1422)
                     /locus_tag="VEP73_08350"
     CDS             complement(76..1422)
                     /locus_tag="VEP73_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYT26472.1"
                     /translation="MAQRSRRRRLHLLPRLAVVLAMLGTGPVPGGGGGAPSAAATMGA
                     APRPAAWRFDGGGYGHGIGMSQYGAYGMGLRGVRAGAILGFYYRGTRLRAVRLPGVRV
                     GVLGARRDPSTGRRLRQVLVRGLAVPGVRFSTGAFTAVGVSRSGRRTARRLRGGVTWS
                     VRSQAGGTSVFAGSRRALGPTRAGSGVSLRFQAGVGTPALLALPQAGRTLRWGRLDVG
                     LVRDGTAIRPRAIAILPFNAYLRGLAEVPGGWPTETLKAQAVAARSYALATVRATGQH
                     RGRRRWDGCDCGVFADTRDQYWAGWAKERGRGGARWVAAVRSTGSHVLTWGSRVVQAF
                     YSSSSGGYTASTASWGGPSLPYFPARRDPDDRARGRNPNYRWRVTRRAATVSAALGRY
                     GVGLVTAMRVASADASGRVRAVEVRGSRRTVTIPGGTFRSLLGLRSTKFKITALAG"
     gene            complement(1522..>2459)
                     /locus_tag="VEP73_08355"
     CDS             complement(1522..>2459)
                     /locus_tag="VEP73_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26473.1"
                     /translation="RPLGERAARLAADRRAQLAAGLAVVAGAVLLLVIGFLRPKLFGV
                     DYFNYNGRVIRSYDEQALHRLSWFFTLPGFALMVAGLALVALRRWVAAAWALVLPGLL
                     LFPVYAYQAQNSSRLMWWSRRFVPVVVPTVVVLIAVALAVALAWSGRWRWPVRLAGAA
                     ATLALLAVFLGQSLPLRGHHEFAGSFETTQRLARLSGGRQGVYLWQPQPGAFAPSSLF
                     ASPIWLQQGEISALLPARPSPAYVRSFVHGFPGQPVFVVAGGGARPAGYGAVPLRLAD
                     RVGERFPIWEESDTTRPSHERMVPVDFSVWRVEGT"
BASE COUNT          353 a          932 c          946 g          228 t
ORIGIN      
        1 cacggcgatc gggggagcat cggcagcggc gccgtgcggg ttgagcccgg atccgcggcc
       61 ccgagcactc atggcctacc cggcgagggc cgtgatcttg aacttggtcg acctgaggcc
      121 gagcagcgac cggaaggtgc cgcccgggat ggtgaccgtc cgcctgctcc cacggacctc
      181 gactgcgcgg acccggccgg acgcatccgc cgacgccacc cgcatggcgg tcaccaggcc
      241 gacgccgtag cggcccagcg ccgccgagac ggtggcggcg cgcctggtga cgcgccagcg
      301 gtagttgggg ttgcggcccc tcgcacggtc gtcggggtcc cggcgggccg ggaagtacgg
      361 caggctcggg ccgccccagg aggcggtgct ggcggtgtag ccgcccgacg acgacgagta
      421 gaacgcctgt accaccctgg atccccaggt caggacgtgc gagccggtcg agcggaccgc
      481 cgcgacccag cgggcgccgc cgcgcccgcg ctccttcgcc cagccggccc agtactggtc
      541 gcgggtgtcg gcgaacaccc cgcagtcgca tccgtcccac cttcgccggc cccggtgctg
      601 gccggtcgcc cgcacggtgg cgagcgcgta gctgcgcgcg gcgaccgcct gggccttcag
      661 cgtctcggtg ggccagccgc ccggcacctc cgcgagaccg cgcaggtagg cgttgaacgg
      721 caggatcgcg atcgcccggg gccggatggc ggtcccgtcc cggaccagcc cgacgtccag
      781 gcggccccag cgcagcgtcc ggcccgcctg cggcaacgcg agcagcgcgg gcgtgcccac
      841 gccggcctgg aaccgcaggc tgaccccgct gccggcccgg gtcggcccca acgcccgccg
      901 cgagccggcg aacaccgacg tccccccggc ctgggagcgt accgaccagg tgacgccgcc
      961 acgcaggcgc cgggcggtcc gccggccgct ccgggagacg ccgacggcgg tgaacgcccc
     1021 cgtcgagaac cggacgccgg ggacggccag gccccggacg agcacctggc gaaggcgccg
     1081 gccggtgctc gggtcgcgcc gggcccccag cacgcccacc cgcacccccg gcaggcggac
     1141 cgcccggagg cgggtcccgc ggtagtagaa gccgaggatg gcgccggcgc gcacgccgcg
     1201 caggcccatc ccgtaggcgc cgtactggct catcccgatc ccgtgcccgt agcccccgcc
     1261 gtcgaagcgc caggcggccg gtcggggggc cgcgcccatg gtggcggccg cggacggggc
     1321 gcccccgccc ccgccgggga ccgggccggt gccgagcatc gcgagcacga cggcgaggcg
     1381 gggcaggagg tggagtcgac gtcggcggga acgctgggcc atgcaggcga cctcccccac
     1441 agcttggtcg aagacaagct agagggtcgg cgccgctccg tggttacttg aacgctcgga
     1501 gtggttgcga gaggatgcgg gtcaggtgcc ctcgacccgc cagaccgaga agtcgaccgg
     1561 gaccatgcgc tcgtgcgacg gccgggtggt gtcgctctcc tcccagatcg ggaaccgctc
     1621 gccgacccgg tcggcgagcc ggagcgggac ggcgccgtag ccggccggcc gcgctccccc
     1681 gccggccacg acgaacaccg gctggcccgg gaagccgtgc acgaacgagc ggacgtaggc
     1741 cgggctcggc cgcgccggga gcagcgcgct gatctcgccc tgctgcagcc agatgggcga
     1801 ggcgaacagc gagctgggcg cgaacgcccc cggctgcggc tgccacaggt acaccccctg
     1861 gcggccgccg gagaggcggg cgagccgctg cgtggtctcg aacgaccccg cgaactcgtg
     1921 gtggccgcgc agcggcagcg actggccgag gaacacggcg agcagggcca gggtggccgc
     1981 cgctccggca agccggacgg gccagcgcca gcggcccgac cacgcgagcg cgaccgccag
     2041 ggcgaccgcg atgaggacca cgacggtggg caccaccacc ggcacgaagc ggcggctcca
     2101 ccacatgagc cggctcgagt tctgcgcctg gtaggcgtag acggggaaca gcagcaggcc
     2161 gggaaggacg agcgcccagg ccgccgccac ccagcggcgg agcgcgacga gtgcgagccc
     2221 ggccaccatg agcgcgaacc ccggcagggt gaagaaccac gacagccggt gcagcgcctg
     2281 ctcgtcgtag ctgcggatga cacggccgtt gtagttgaag tagtcgaccc cgaagagctt
     2341 cgggcgcagg aagccgatca cgaggagcag taccgccccg gccacgacgg ccagcccggc
     2401 ggcgagctgg gcgcgccggt cggcggccag gcgggccgcc cgctcgccga gcggccggg
//
LOCUS       DASPYY010000527         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_369349, whole genome shotgun sequence.
ACCESSION   DASPYY010000527 DASPYY010000000
VERSION     DASPYY010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369349"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VEP73_08360"
     CDS             complement(<1..1106)
                     /locus_tag="VEP73_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYT26474.1"
                     /translation="MDRHAHTVTPTAPRQHGGIRIAGFRVRIGWGAAATAVLIGWSLA
                     SGVFPAAFPGRAGDAYWVAGLATAGLFLASVLAHELAHALAARRAGLAVEDITLWIFG
                     GVARIHGDAPTPAAALRIAAVGPLASAVLAAGFAIGAVALSVLGVGGLAAGVVTWLAG
                     MNAVLAVFNLLPGAPLDGGRILGAWLWRRHGDRVRAAVTAARAGQAIGVALVAVGVGE
                     LLLGRGAGGIWNALVGWFVYAAAQGERRHAELERGLGRLRVGDVMLPDPPVGPAWFTV
                     EAFLDGYAARHRLRAFPLHEFDGRPAGLVTPPALLRVPPERRSAVRVGAVAVPAAEVV
                     TASPGEPVVELLRRLGAAGQVWALVVDGGRLVGIV"
     gene            complement(1294..1863)
                     /locus_tag="VEP73_08365"
     CDS             complement(1294..1863)
                     /locus_tag="VEP73_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26475.1"
                     /translation="MQETGDLVRSAAAGDQRSWGALVERFTGLLWAVARAHRLRDADA
                     ADVVQTTWLNLAQHLGSIREPDRVGAWLATTARRECLRVLNRDRCQVPTDDEASLQPR
                     EPPAASTPEDEVLLLERHALLRAAIQDLPPRYQQLLRVLVADPPPSYEAVGAALDMPV
                     GSIGPTRARCLELLRRSPRLAGIVPARIP"
BASE COUNT          296 a          826 c          858 g          214 t
ORIGIN      
        1 acgatgccga ccaggcggcc gccgtcgacc accagcgccc acacctggcc ggcggcgccg
       61 agacgccgca gcagctcgac gaccggctcg cccgggctgg ccgtcacgac ctcggccgcg
      121 ggcaccgcga cggcgccgac ccgcaccgcc gaccggcgct cgggcggcac ccgcaggagt
      181 gcgggcggcg tgaccaggcc ggccgggcgg ccgtcgaact cgtgcagggg gaaggcgcgc
      241 aggcggtgcc gcgcggcgta gccgtccagg aacgcctcga ccgtgaacca ggccgggccg
      301 accggcgggt cggggagcat cacgtcgccg acccgcaggc gaccgagccc ccgctccagc
      361 tcggcgtgcc ggcgctctcc ctgcgccgcg gcatagacga accagccgac cagcgcgttc
      421 cagatgccgc cggccccgcg gccgaggagc agctcgccga cgccgacggc gacgagcgcg
      481 acgccgatcg cctggccggc ccgtgccgcg gtgacggccg cccggacgcg gtcgccgtgc
      541 cggcgccaca gccacgcgcc gaggatgcgc ccgccgtcca gcggagcgcc cgggagcagg
      601 ttgaacacgg cgaggacggc gttcatcccg gcgagccagg tcaccacccc ggcggcaagg
      661 cccccgacgc cgaggaccga cagggccacc gccccgatcg cgaacccggc cgcgagcacg
      721 gcgctcgcga gcgggccgac cgcggcgatg cgcagcgccg ccgccggggt cggcgcgtcg
      781 ccgtggatcc gcgcgacccc gccgaagatc cacagggtga tgtcctccac cgcgagcccg
      841 gcccgcctgg cggccagcgc atgcgcgagc tcgtgggcga gcacggaggc gaggaacagc
      901 ccggcggtcg ccaggccggc gacccagtag gcgtcgccgg cgcgccccgg gaaggcggcc
      961 gggaagacgc cggacgcgag cgaccagccg atgagcacgg cggtggcggc tgcgccccag
     1021 ccgatccgga cacggaagcc ggcgatgcgg atcccgccgt gctgccgcgg cgcggtcggt
     1081 gtgacggtgt gagcgtggcg atccatcgaa tacgtccctt cggctcggct gcgcgcttgc
     1141 agcgccgaag gtctctcccg cccggaacgg gccggggcac cgggccgagg ccgtgatgac
     1201 gatgccgccg agaaagctgg ggtactcccc ttcgcacggc catggtagcg cgaggagccg
     1261 tcaaggcggc gtaaagcgcg gcgcgtgtca gggtcagggg atccgcgcgg ggacgatgcc
     1321 ggccaggcgg ggggagcgcc gcagcagctc gaggcagcgg gcgcgggtcg gcccgatgct
     1381 gccgaccggc atgtcgagcg ccgcgccgac cgcttcgtag ctgggcggcg ggtcggcgac
     1441 gaggacccgc aggagctgct ggtaccgcgg cggcaggtcc tggatcgcgg cgcggaggag
     1501 ggcgtggcgc tcgagcagca gcacctcgtc ctctggcgtc gacgccgccg ggggctcgcg
     1561 cggctgcagg ctggcctcgt cgtcggtcgg gacctggcac cggtcgcggt tcaggacccg
     1621 caggcactcc cggcgcgctg tggtcgcgag ccacgcgccg acgcgatcgg gctcccggat
     1681 gctgccgagg tgctgcgcga ggttcagcca ggtggtctgg acgacgtcgg cggcgtcggc
     1741 gtcccggagg cgatgcgccc gcgccaccgc ccagaggagc ccggtgaagc gctcgacgag
     1801 cgcgccccac gaccgttggt cccccgcggc ggcagaccgg acgagatcgc cggtctcctg
     1861 catctcagct gcctcccctc agcagggcat gcccgctgac gcccgagact accggccgag
     1921 gccgccggca tcgcgcggcc acgcaacact cgaagtgtca cgcgcgcgac attccggctg
     1981 gccgcgcgtt cggaggcgtg ccgagcggga cagggagctc ggaggcggcg cgcgctcagc
     2041 gcgcccggcg ggtcagcgcg tcgaggtcga cgatctcgac ccgtgaccgc ccgacggcga
     2101 cggcgccggc cagctccacc gccctgagcg tgtggttcgt ggtcgaccgg gacgtccccg
     2161 cgaggctgac caggacctcc tggttgaggg ggat
//
LOCUS       DASPYY010000528         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2197234, whole genome shotgun sequence.
ACCESSION   DASPYY010000528 DASPYY010000000
VERSION     DASPYY010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197234"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEP73_08370"
     CDS             <1..358
                     /locus_tag="VEP73_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HYT26476.1"
                     /translation="AAVPPLHRIKLISPRKGQRKYIYTYSDSEMHRTLLELERKGRRW
                     EDPPQRYKGLGEMDAAQLAETTMDPRHRTLRRIRIEDAAAATAMFDLLMGSEVAPRRD
                     FIVGSAAELDPARIDA"
     gene            544..1731
                     /locus_tag="VEP73_08375"
     CDS             544..1731
                     /locus_tag="VEP73_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT26477.1"
                     /translation="MTQAELFEGVALADGLSPALRTPVSVLVGGGILAGIYDPEAPAE
                     ARAGARVIDASGATLVPSFVDCHSHLTLQGGAQWVERGADDTAELIDAAERNAAALVA
                     AGVRWVRDVGSPRRADTPDGRTRALALSLRDQWQGKADYPYVRAAGTWLAKGGSLPAG
                     LAVEAEDADELLAISLGQLDDGADLIKLYLDGPDADVAPWTADEVRRVVDAVHARGAT
                     VAAHGTGLANCRLAADAAVDTLEHGFTIDSDVARTLASNGVTVVSTLSVLHSWQGFAT
                     TTTIERFTGQDGIRRIAERREVAEASIREAHAAGVAIAAGSDFGGGSVRAGHLAWEVE
                     GLVRAGLEPWEALGAATWRGGDVLGDPHAGRLTVGGPAHFTLVHGDPLADPAALWRVW
                     RAL"
BASE COUNT          239 a          636 c          657 g          206 t
ORIGIN      
        1 cgccgcggtc ccgccgctgc accgcatcaa gctgatcagc ccccgcaagg gccagcggaa
       61 gtacatttac acgtactccg attccgagat gcaccgcacg ctgctcgagc tggagcgcaa
      121 gggccggcgg tgggaggacc cgccgcagcg gtacaagggc ctcggcgaga tggacgccgc
      181 ccagctggcc gagaccacca tggatccgcg tcatcgcacg ctgcgccgga tcaggatcga
      241 ggacgccgcg gccgcaacgg ccatgttcga cctgctgatg ggcagcgagg ttgcgccgcg
      301 cagggatttc atcgtcggca gcgccgccga gctcgaccca gcgcggattg acgcctgacg
      361 gcgccagcgg cggccggggg gcggggcggc cagccccggg taagccggcc ggctggccgg
      421 gggcctggcc gcacaagccc cggcaggctc gtccggctca ggggttgacc cggccgccgc
      481 cgggcgtcga cccttggagc cgggcaaccc gcccgagaac cagccgaccg gaaaggatcg
      541 ccggtgaccc aggcagagct cttcgaaggc gtcgcgctcg cggacgggct cagcccggcg
      601 ctgcggacgc cggtcagcgt cctcgtcggc ggcggcatcc tggccggcat ctacgaccca
      661 gaggcgcccg ccgaggccag ggcgggcgcc agggtcatcg acgcgagcgg cgcgaccctg
      721 gtcccgtcgt tcgtggactg ccacagtcac ctcacgctgc agggcggcgc gcagtgggtc
      781 gagcgcggcg ccgacgacac cgccgagctg atcgacgccg ccgagcggaa cgccgccgcg
      841 ctggtggcgg ccggcgtccg ctgggtccgt gacgtcggct cgccgcgccg cgccgatacc
      901 cccgacgggc gcacccgcgc gcttgccctc tccctgcgcg accagtggca gggcaaagcc
      961 gactatccct acgtgcgcgc cgcaggcacc tggctggcca aaggcggatc cctgcccgca
     1021 ggcctggcgg tggaagccga ggacgccgat gagctgctcg ccatctcgct cggccagctc
     1081 gacgacggcg ccgacctgat caagctgtac ctggacggcc cggacgccga cgtcgccccg
     1141 tggacggcgg acgaggtgcg ccgggtcgtc gacgccgtgc acgcgcgggg cgcgacggtc
     1201 gccgcgcacg gaaccgggct ggccaactgc cggctcgcgg ccgatgcggc cgtggacacg
     1261 ctggaacacg ggttcacgat cgactcggac gtggcgcgga cgctcgcgtc caacggcgtg
     1321 accgtggtgt cgacgctctc ggtcctgcac tcgtggcagg gcttcgccac gaccacgacc
     1381 atcgagcggt tcaccggcca ggacggcatc cggcgcatcg ccgagcggcg tgaggtggca
     1441 gaggcgagca tccgcgaggc gcatgcggcc ggcgtggcga tcgccgccgg gagcgacttc
     1501 ggcggcggct cggtgcgggc cggtcatctt gcctgggagg tggagggact ggtccgtgcc
     1561 gggctggagc cctgggaagc cctcggcgcg gccacctggc ggggcggtga cgtgctcggc
     1621 gatccgcacg cgggcaggct cacggtcggc ggcccggcgc acttcacgct ggtccacggc
     1681 gatccgctcg ccgacccggc cgccctctgg cgggtctggc gtgcgctcta aagcgaca
//
LOCUS       DASPYY010000529         8007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1354989, whole genome shotgun sequence.
ACCESSION   DASPYY010000529 DASPYY010000000
VERSION     DASPYY010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354989"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1046
                     /locus_tag="VEP73_08380"
     CDS             81..1046
                     /locus_tag="VEP73_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT26478.1"
                     /translation="MSHPMDAAVTLSLTGPFARQGRDAADGLALWAEEAGVRLAVTDD
                     NGSRSTATRAYAAWLDGGVELLIGPYGSGLVRAVAPLVCASGRLLWNHGGSADDLARP
                     GLVSLPAPASTYFDGAVDQALARRVERLVIVQGAGPFARAVAEGAGRRAATLGMDART
                     VHAGTLTAGEAAGAAVLAAGRFEEDVALVRRLGCLPGGAALVAAVAAGLPAFGELAEA
                     AEGVLGPVQWWPASRTPGVGPSGAGFAARYRRRVGREPSYVAAQAAAAGYLAHAAHRL
                     GPAAGAPRQWTTSTLLGDFALDAAWRQVGHRVTTVRWQGGRMVPL"
     gene            1065..1433
                     /locus_tag="VEP73_08385"
     CDS             1065..1433
                     /locus_tag="VEP73_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT26479.1"
                     /translation="MAEVVDEGTFDPEEFRDELVAAAGNLDVGTRLWFESDRVKVWEI
                     RLAPGERGPFHAHTRRYLWTVVEAGTGLQRSPDGTFTVRPYEVGDTRYLEPSAADPLV
                     HDLENVGDTTLRFVTVELLS"
     gene            1445..1966
                     /locus_tag="VEP73_08390"
     CDS             1445..1966
                     /locus_tag="VEP73_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HYT26480.1"
                     /translation="MSFKVAKFLDVSQGIQENQFTVGSREEKESLEDLDPVYRQLLDE
                     PVTAVIAVTGGDGRPWLTPVWFNYDGDKVLVNLAEHRRKTEWIRRDPRVTMLLMNPKN
                     PYHWLSIKATVEREIHEDDPAEGHKATETIDRAWTKYTGNEPPYGLRDPAMDERRVLF
                     ECRVDSVATFGQP"
     gene            2039..3226
                     /locus_tag="VEP73_08395"
     CDS             2039..3226
                     /locus_tag="VEP73_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT26481.1"
                     /translation="MSGWPRVAVAGGSLGGLTAALVLRDLGCQVDVFERSTMELQSRG
                     AGIVVLDATVRYFRERTGLDLDRITTSTGLLRYLGQDGAVVHEEPRRHRYSAWHTIYR
                     ALLGCLGRDSYHLGRAVGGFRQGADHVVVELTDGAAAEYDLLVCADGISSSARARLQP
                     RARPAYAGYVAWRGTVAEPQLGRGVRDLLADSIVYQVIPSSHILLYPIPALDGSVEPG
                     RRLANFVWYRNYAAGEELDDLMTDRDGARREITLPPGAAREAHLRELRTFAAGRLAPQ
                     FAELVHRTELPFVQVIFDIEVTRMAFGRVCLLGDAAFALRPHAAAGTAKAAADAWALA
                     EALAATRGDVAAALPRWERRQLAVGRAVLERARRNGDKSQFEGTWRPGDPELAFGLYE
                     PGR"
     gene            complement(3254..3469)
                     /locus_tag="VEP73_08400"
     CDS             complement(3254..3469)
                     /locus_tag="VEP73_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYT26482.1"
                     /translation="MVDSVYRITEVVGTSSESWEKAASAAVETAAKTLRDLRIAEVVQ
                     LDMAVEDGQVRAYRARVKISFKLQGRE"
     gene            4011..4400
                     /locus_tag="VEP73_08405"
     CDS             4011..4400
                     /locus_tag="VEP73_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT26483.1"
                     /translation="MPLEVTAIMIGVEDLARSKKFYGEGLRCTIDQDHPNFVSFNLGD
                     GSSSLALYQWEAAAQDAGVSSEGSGFRGVSFHFIVPSSEAVDEVMSSAVAAGGGVVKE
                     AAASQWGGYFGYFSDPDGYLWKVASTS"
     gene            complement(4675..4950)
                     /locus_tag="VEP73_08410"
     CDS             complement(4675..4950)
                     /locus_tag="VEP73_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26484.1"
                     /translation="MATDLQGRRVPQIVQPQPLGKRIGAAMLVELGDHLVGGTDARLE
                     PAGDELRLAQRPTQWRGEDQILPGRAAGQVGAQLVGDRARQPDRAAQ"
     gene            complement(4960..5044)
                     /locus_tag="VEP73_08415"
     tRNA            complement(4960..5044)
                     /locus_tag="VEP73_08415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5008..5010),aa:Leu,seq:taa)
     gene            complement(5101..5715)
                     /locus_tag="VEP73_08420"
     CDS             complement(5101..5715)
                     /locus_tag="VEP73_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HYT26485.1"
                     /translation="MVLESACIPIPSEVTMLFGGALANATFVTTVAGGEQHHLNFVVV
                     GLAGALGNLVGSWIAWGVGYAGGRPLIERWGRYVFLRPHELDRAEVWFQRHGPAAVFV
                     SRLLPVVRTFISLPAGIAEMPLPRFSVYTFAGCLPWTFALAAVGYAVGSQWTRVEHLL
                     KPVTYVIAAVVVVWAAVWFLRRAADQRRRDADKAGRHEATSGRR"
     gene            complement(5843..6571)
                     /locus_tag="VEP73_08425"
     CDS             complement(5843..6571)
                     /locus_tag="VEP73_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYT26486.1"
                     /translation="MSTTLSPAIQEYLEALYWLHEAGIECSPTNLSRAMQVSPPSVTE
                     MVRKLEVEGLVTRGPGKRISLTEPGEQVARHVVNRHRLVEAFLVKVMGVPWDEVHEEA
                     ETFEMGVTETLEARMLDMIGEMRTCPHGHPIGDFPREPGVPLTSVPAGSAIRVLRFEN
                     EDHDLLRAFMRAGVEPRSTWTVTDFADGHALLERDGRRTSLELHAARTISVSVERAGA
                     GEPAERDPESLATAYLLGDSHWSR"
     gene            complement(6619..6984)
                     /locus_tag="VEP73_08430"
     CDS             complement(6619..6984)
                     /locus_tag="VEP73_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYT26487.1"
                     /translation="MPRRSRPLAPLLVLAFTILAAACQPNHPDNRAQQATPVTGVREV
                     VARNLAFTPPAIQVPAGTTVTWRFDDGSVPHNVQGDGYSSKTASRGTFTHRFDRPGSY
                     EYRCTLHAGMTGRVVVVPR"
     gene            7152..7340
                     /locus_tag="VEP73_08435"
     CDS             7152..7340
                     /locus_tag="VEP73_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26488.1"
                     /translation="MTTWTIEGPQKLTFDRVRMRKVRTIGGTLSVVGTDDQQLLEGRL
                     GAGDRGRLGAGREPEVAT"
     gene            7337..>8007
                     /locus_tag="VEP73_08440"
     CDS             7337..>8007
                     /locus_tag="VEP73_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYT26489.1"
                     /translation="MSSVFSHGRLRLYLLKLLEESPRHGYEVISLLEDRFMGLYAPSP
                     GTVYPRLQRLEAEGLVTHSQEGGRKVYRITDAGREELDRRRDEVADLEADIRGSVRDL
                     AREIRDEVRGTVRNFKQEVKQAAREMRREQRHEHRNRRDSYGAAWSDRSEGAAGAGAG
                     TANGTNGPATAGGKRLDQRLQEFLEGVAPLMQRAYVTEAQLRECVAILDEAFGRIRET
                     LRKAP"
BASE COUNT         1117 a         2787 c         2993 g         1110 t
ORIGIN      
        1 ctcctgacac gccccggcgc ggacgagccg cccggccggg ctgccccagg aacgcggacg
       61 agccgtccag gaggggggtc atgagccacc ccatggacgc ggcggtcacg ctgagcctca
      121 cggggccgtt cgcgcgccag ggacgggacg cggccgacgg gttggcgctg tgggccgagg
      181 aggccggcgt ccggctggcc gtcaccgacg acaacggctc gaggtcgacg gcgacacggg
      241 cctacgccgc ctggctggac ggtggcgtcg agctgctgat cggtccgtac ggcagcggcc
      301 tggtccgggc ggtggcgccg ctggtgtgcg cttccgggcg gctgctgtgg aaccatggcg
      361 gctcggccga cgacctcgcg cggccggggc tggtgtcgct cccggcgccc gcctcgacct
      421 acttcgacgg tgcggtggac caggcgctgg cccgccgggt cgagcggttg gtgatcgtcc
      481 agggggccgg accgttcgcg cgggccgtcg ccgagggggc cgggaggcgt gcggccacgc
      541 tggggatgga cgcacgcacg gtccacgccg gcacgctgac cgccggggag gcagccggcg
      601 ccgcggtcct ggccgccggc cgcttcgagg aggacgtggc gctggtccgc cgcctcggct
      661 gcctgccggg aggtgcggcg ctggtggctg ccgtggcggc ggggctgccg gccttcggcg
      721 agctggcgga ggcagcggag ggcgtgctcg ggccggtgca gtggtggccg gcctcgcgga
      781 cgccgggggt cgggccgtcg ggggccggct tcgccgctcg ctaccggcgc cgggtcgggc
      841 gcgagccctc gtacgtggcc gcccaggcgg cggccgccgg ctacctcgcg cacgccgccc
      901 accggctggg tccggcggcc ggcgcgccgc ggcagtggac gacctcgacg ctgctcgggg
      961 acttcgccct ggatgccgcc tggcggcagg tcggccaccg cgtgacgacc gtccggtggc
     1021 agggcggccg catggtcccg ctctgattga tctagagttg cggcatggcc gaggtcgtgg
     1081 acgagggtac cttcgatccc gaggagttcc gggacgagct cgtggccgcc gcgggcaacc
     1141 tggacgtggg cacgcgactg tggttcgaga gcgaccgggt caaggtgtgg gagatcaggc
     1201 tcgcgccggg cgagcggggg ccgttccacg cccatacgcg gcggtacctc tggaccgtgg
     1261 tggaggccgg gaccggcctg cagcgctcgc cggacggcac cttcacggtc cggccctacg
     1321 aggtgggcga cacccggtac ctggagccct ccgcggcgga cccgctggtc cacgacctcg
     1381 agaacgtcgg cgataccacg ctccgcttcg tcaccgtgga gctgctgagc tagaggaggg
     1441 tgccgtgtcc ttcaaggtcg ccaagttcct cgacgtctcc caggggatcc aggagaacca
     1501 gttcacggtc gggtcccggg aggagaagga gtccctcgag gacctcgacc cggtgtaccg
     1561 gcagctgctc gacgagccgg tgacggccgt gatcgccgtg acgggcgggg acgggcgccc
     1621 ctggttgact ccggtctggt tcaactacga cggggacaag gtgctcgtca acctcgccga
     1681 gcaccgccgc aagaccgagt ggatccggcg cgatccccgg gtgaccatgc tcctgatgaa
     1741 cccgaagaac ccgtaccact ggctcagcat caaggccacg gtcgagcggg agatccacga
     1801 ggacgacccc gccgaggggc acaaggcaac cgagaccatc gaccgggcgt ggaccaagta
     1861 cacgggcaac gagcccccct acggcctccg cgacccggcc atggacgagc gtcgcgtgct
     1921 gttcgagtgc cgtgtcgaca gtgtcgccac cttcgggcaa ccctagccat ccgtcgcccc
     1981 cccaggcggg gtggcccgtg cgtcgcggga atcgcgaccg ccctggacgg gcttcctggt
     2041 gagcggctgg ccacgggtcg cggtggcggg gggctcgctc ggggggctca ccgccgcgct
     2101 cgtcctgcgt gacctcggct gccaggtcga cgtgttcgag cggtcgacca tggagctaca
     2161 gagccgcggc gcggggatcg tcgtcctgga cgcgaccgtc aggtacttcc gcgagcggac
     2221 cggcctcgac ctggaccgga tcaccacgtc gacggggctc ctccggtacc tcggccagga
     2281 cggcgcggtc gtccacgagg agccgcggcg ccaccggtac tcggcctggc acacgatcta
     2341 ccgcgcgctg ctcggctgcc tggggcggga ctcgtaccac ctcggcaggg cggtgggcgg
     2401 cttccgtcag ggagccgacc acgtcgtggt ggagctcacc gacggcgcgg cggccgagta
     2461 cgacctgctg gtctgcgccg acggcatctc gtccagcgcc cgcgcgcggc tgcagccgcg
     2521 cgcccgaccc gcctacgccg gctacgtggc ctggcggggg acggtcgccg agccacagct
     2581 cggccgtggc gtgcgcgacc tgctcgccga ctcgatcgtc taccaggtca tcccctcgag
     2641 ccacatcctc ctctacccga tccccgccct cgacgggtcg gtggagcccg gccgccgcct
     2701 ggccaacttc gtgtggtacc ggaactacgc cgccggggag gagctcgacg acctgatgac
     2761 cgacagggac ggcgcccgcc gcgagatcac cctgccaccg ggtgcagccc gcgaggcgca
     2821 cctgcgcgag ctgcggacct tcgccgccgg gcgcctcgct ccgcagttcg ccgagctcgt
     2881 gcaccgcacc gagctcccgt tcgtccaggt gatcttcgac atcgaggtca cccggatggc
     2941 cttcgggcgc gtctgcctgc tcggcgacgc cgccttcgcc ctgcgcccac acgccgccgc
     3001 ggggacggcc aaggcggcgg ccgacgcctg ggcgctggcc gaggcgctcg ccgcgacgcg
     3061 gggcgacgtc gcggcggcgc tgccgcggtg ggaacgccgg cagctcgccg tcggacgcgc
     3121 ggtgctcgaa cgggcccgca ggaacggcga caagtcgcag ttcgaaggga cctggcgccc
     3181 cggggacccg gagctggcct tcggcctgta cgaaccaggg cgctgacccg ccgcggtcac
     3241 cggcgaaccg agctcactcc cggccttgga gcttgaacga gatcttcacc ctggcccggt
     3301 aggcccgcac ctgcccgtcc tcgaccgcca tgtcgagctg cacgacctcg gcgatgcgaa
     3361 ggtcgcgcag cgtcttcgcc gccgtctcca cggccgcgct ggccgccttc tcccaggact
     3421 cgctgctggt cccaacgacc tcggtgatcc ggtagacgct gtccaccatt gccatccctc
     3481 cgatcgacga ctggccatcg cgtactctcg ctcggcggcg gcgcgcccgc aaggccacgt
     3541 gccatcgagg gcccccgagc cgggccatcc ctgtggctct ggcaggaggg cacatgggcg
     3601 aacggaagat ccggatgcag taccaccgcg gcgtcagcga gcggctcctc acgggctcgc
     3661 tcgagcaacg aggactatga cctcgcctac ggtgaggagt tcatccgtaa ccggctcgag
     3721 gccgagcagg accttgccgc cgggcggaca cacgcacccg gcgacgtgct ggccgagttg
     3781 gacgaccgac tggcagtgcg caccctcgtc gagctgtccc gccggccacc tgttccccgc
     3841 gcggttccgg tgccgtctgg tcgcggacac ctctagtgat ccttcgctgg aatactatgg
     3901 cgggccaagg tggcgcccag ccacactttg ccgttttgtt tcgtcataga ggttgagttc
     3961 gtcatagagg ttgagatggt caacgcagcg tacgagaagg gagacaaacc gtgcctctgg
     4021 aagtgaccgc gatcatgatc ggtgtcgagg acctggcccg ctcgaagaag ttctatgggg
     4081 aaggcttgcg ctgcacgatc gaccaggacc atcccaactt cgtgtcgttc aacctgggtg
     4141 acggatcgtc gtcgctggcc ctctaccagt gggaggcagc ggcccaagac gccggcgtgt
     4201 cctctgaggg ttccggcttc cggggcgtgt cgttccattt catcgtcccg tcgagcgaag
     4261 ctgtggacga ggtcatgagc agtgccgtgg ctgccggtgg cggcgtcgtg aaggaagcgg
     4321 cggcctccca gtggggcggg tacttcgggt acttcagcga cccggacggc tacctctgga
     4381 aggtcgcgtc cacctcctaa ctctcaccgt ggtcgccagc gcaggcgcag acaggcgtca
     4441 tcagctgggc ggcctgacca gaccggcccg gccgggtagc ggtgggctac gtcgaggacc
     4501 tgcggggcaa gaagcagggc cgcggccggc ctcgctggcg ggcgcgctac cgtgacccgt
     4561 ccggccggga gcgggccaag agcttcgccc gcaaggtgga cggcggtgcg ggagtgggtc
     4621 gccgggggcc tgtcggcctg cgagttcgtc gcgctgaagg tcgggcggct cgacctactg
     4681 cgcggcacgg tccggctgcc gcgctcggtc gccgacgagc tgggcgccta cctggccggc
     4741 cgcacggccc gggaggatct gatcttcacc gcgccactgg gtgggccgct gcgcgagtcg
     4801 gagttcgtcc ccggccggtt caagccgggc atccgtgccg ccaacgagat gatcgccaag
     4861 ctcgacaagc atggccgccc cgatccgctt cccgaggggt tgcggctgca cgatctgcgg
     4921 cacacggcgg ccttgcaggt cagtggccat gctagggggt gccggaggag gggctcgaac
     4981 cctcatgccc ttgcgggccc aacgctttta aggcgcggct gtctgccgat tccagcactc
     5041 cggcagggtg cgagcttcga gcctacggga accgggcacg tccgggcgag ccgcggaagg
     5101 tcagcggcgc cccgacgtgg cctcgtggcg cccggccttg tcggcgtccc tgcggcgctg
     5161 gtccgccgcg cggcgcagga accagacggc ggcccagacc accacgacgg ccgcgatcac
     5221 gtaggtcacc ggcttcagca ggtgctcgac gcgggtccac tggctgccga ccgcgtagcc
     5281 gaccgcggcc agggcgaacg tccagggcag gcagccggcg aacgtgtaca ccgagaaccg
     5341 cggcagcggc atctcggcga tgccggcggg caggctgatg aaggtccgca cgaccggcag
     5401 caggcgggag acgaacacgg cggccggacc gtggcgctgg aaccagacct cggcccggtc
     5461 cagctcgtgc gggcgcagga agacgtagcg gccccagcgc tcgatcagcg ggcggccgcc
     5521 ggcgtagccg acgccccacg cgatccagga cccgaccagg ttgccgagcg caccggccag
     5581 gccgacgacg acgaagttga ggtggtgctg ctcgccgcct gccacggtgg tcacgaaggt
     5641 cgcgttggcc agcgccccgc cgaacagcat cgtcacctcg ctcgggatcg ggatgcaggc
     5701 cgactcgagg accatgagga cgaagacggc gaggtagccg tagtcgagga tcgtcttggt
     5761 gatgaactcg gtcacaactg tgggctcctg gtcgggccgg gacggtcgcc ggtggtgccg
     5821 gcatgctacc tggtcggccc ggtcagcggc tccagtggct gtcaccgagc aggtaggcgg
     5881 tggcgaggct ctccgggtcg cgctcggcgg gctcgccggc gcccgcccgc tccacgctga
     5941 cagagatcgt gcgggccgca tggagctcga gggacgtgcg gcggccgtcc cgctcgagca
     6001 gcgcatggcc gtccgcgaag tcggtcaccg tccaggtcga cctgggctcg acgccggccc
     6061 gcatgaacgc cctgagcagg tcgtggtcct cgttctcgaa gcgcagcacc cggatcgcgc
     6121 tgccagccgg cacgctggtg aggggcacgc ccggctcgcg cgggaagtcg ccgatggggt
     6181 gcccgtgggg gcaggtccgc atctcgccga tcatgtcgag catgcgtgcc tcgagcgtct
     6241 cggtcacgcc catctcgaag gtctcggcct cctcgtggac ctcgtcccag gggacgccca
     6301 tgaccttgac gaggaacgcc tcgaccagcc ggtggcggtt tacgacgtgc ctggcgacct
     6361 gctcgccggg ctcggtcagc gagatgcgct tgcccgggcc acgggtcacg aggccctcga
     6421 cctcgagctt gcgcaccatc tcggtcacgc tgggcgggga gacctgcatc gcgcgggaga
     6481 ggttcgtcgg gctgcactcg atcccggcct cgtggagcca gtagagggcc tccaggtact
     6541 cctggatcgc cgggctcagc gtcgtgctca tggccatatc ctacgcggcg ccgggccgcc
     6601 cccaacaggg cgccgccact acctggggac gaccaccacg cggccggtca tgccggcgtg
     6661 cagggtgcag cggtactcgt agctgcccgg gcggtcgaac cggtgggtga acgtgcccct
     6721 gctcgcggtc ttcgacgagt agccgtcgcc ctggacgttg tgcgggacgc tgccgtcgtc
     6781 gaagcgccag gtgacggtgg tcccggccgg cacctggatc gccggcgggg tgaaggcgag
     6841 gttcctggcg acgacctcgc ggacgccggt gaccggcgtc gcctgctggg cgcggttgtc
     6901 gggatggttg ggctggcacg cggcggccag gatcgtgaac gcgagcacaa gaaggggggc
     6961 aagggggcgt gatctgcgcg gcactgggtc tcctgaggtc cgaggtccac ccgcctgaag
     7021 cgtgcgaggc cgtgccagtc tgccaccggc ggcggcccgc cgccccgcgc agccgcggcg
     7081 cctcttgaca gcggcataac gagatatatc gtgtagggag gatcacgcga tgtatcgtga
     7141 agggggcgac cgtgaccacc tggaccatcg aggggccgca gaagctcacc ttcgatcggg
     7201 tgcgcatgcg taaggtccgc accatcgggg gaacgctgag cgtcgtgggc accgacgatc
     7261 agcagctcct cgagggacgg ctcggcgccg gcgaccgggg gcggctcggt gccggcaggg
     7321 aacccgaggt ggcgacgtga gctccgtctt cagccatggc cggctgcgcc tgtacctgct
     7381 caagctgctg gaggagagcc cgcggcacgg gtacgaggtg atcagcctgc tcgaggaccg
     7441 gttcatgggc ctgtacgcgc cgtcgccggg caccgtgtac ccgcggctcc agcgcctgga
     7501 ggccgagggc ctggtcaccc actcccagga gggcggccgc aaggtgtacc ggatcaccga
     7561 cgccggccgc gaggagctcg accggcggcg ggacgaggtc gccgacctgg aggccgacat
     7621 ccgcggctcc gtgcgcgacc tggccaggga gatccgcgac gaggtccgcg gcaccgtccg
     7681 caacttcaag caggaggtca agcaggccgc cagggagatg cgccgcgagc agcggcacga
     7741 gcaccgcaac cgacgggact cctacggggc ggcgtggtcc gaccggtccg agggggccgc
     7801 cggggcggga gccggcaccg cgaacgggac caacggtccg gcgacggcgg gcgggaaacg
     7861 cctcgaccag cggctgcagg agttcctcga gggggtggcc ccgctgatgc agcgggcata
     7921 cgtgaccgag gcgcagctcc gcgagtgcgt cgccatcctc gacgaggcgt tcggccgcat
     7981 ccgcgagacg ctgcgcaagg cgccctg
//
LOCUS       DASPYY010000530         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_370457, whole genome shotgun sequence.
ACCESSION   DASPYY010000530 DASPYY010000000
VERSION     DASPYY010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370457"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VEP73_08445"
     CDS             <1..1291
                     /locus_tag="VEP73_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HYT26490.1"
                     /translation="RKGDSAESVLDGLDAGVELRLLAHVTLAVAVDAAVVAGHAAEDA
                     PLPLPHWRAPRGGAGPWLGRVGATFRTHLSPSTVWFRNSLRAALALGLAILAARTVRV
                     DHSFWVVLGTLSVLRSNALATGRTALLAILGTVAGFIVAAGLTLAVGTSRAGLWIALP
                     VTVFLAAYVPTAVSFLIGQAAFTLYVIVLFDLLQPQGWRLGLVRMEDVLLGAAVSLVV
                     AVLLWPRGARGQLRSALATLYRADAACLAAAFGYLLGDRTEPEVDASRRAARIEVARA
                     GEAFDEFLTERGSRTLATVTWGRIAAAGDDLLLAVDAMEVMSLLGYRTDGCEDCVGRV
                     RRDVDAVVGVFAGFADELEAARQPPGPRVEAAAATRDAVLQCLRAWGGAPGSPLDRTA
                     IGLAAASFWSVEIARIGGELAEPLRSVAVAASSPWWR"
     gene            1418..2041
                     /locus_tag="VEP73_08450"
     CDS             1418..2041
                     /locus_tag="VEP73_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HYT26491.1"
                     /translation="MADEARLTAEELNAVELTKAMRGYAMHEVDTFLDRAVQELARLQ
                     AKQSGEYAPSTPRLTPEAIQHKVFTKALRGYAMQEVDALLDRLADELARLQREERWMQ
                     ASLTGRPAAMPGITPEEIQRKQFTVALRGYVMAEVDDFLLEAARELARLRAERSRGYR
                     PSPDRLTADNLRHWRFSKAMRGYAVHEVDQFLDRLAGEVGRLLRDPT"
BASE COUNT          261 a          787 c          786 g          297 t
ORIGIN      
        1 tcgcaagggt gactcggccg agtcggtgct cgacgggctc gacgccgggg tggagctgcg
       61 actgctcgcc cacgtcaccc tagccgtcgc ggtcgacgcc gccgtggtgg ccggccacgc
      121 cgcggaggac gcgccgctgc cgctgccgca ctggcgggcg cctcgcggcg gcgccgggcc
      181 gtggcttggc cgcgtgggcg ccacgttccg cacgcacctg tcgccgagca ccgtgtggtt
      241 ccgcaacagc ctccgagccg ccctcgccct cgggctcgcc atcctcgccg ccagaacggt
      301 gcgcgtggac cacagcttct gggtggtgct gggaacgctg tcggtccttc gttcgaacgc
      361 actcgccacg gggcggaccg ccctgctcgc catcctcggg acggtcgctg ggttcatcgt
      421 ggcggccggc ctcacgctgg cagtggggac cagcagggca gggctgtgga tcgcactgcc
      481 cgtcaccgtg tttctcgccg cctacgtgcc caccgcggtg tcgttcctga tcggtcaggc
      541 cgcattcacc ctctacgtga tcgtcctgtt cgacctcctc cagccgcagg gctggcggct
      601 cgggctggtc aggatggagg acgtgctgct cggggccgcg gtgagcctgg tcgtcgccgt
      661 gctgttgtgg ccgcgggggg ccaggggcca gcttcggtcc gccctcgcga cgctgtaccg
      721 ggccgacgcc gcgtgcctcg ccgcggcgtt cggctacctg ctcggcgatc ggaccgagcc
      781 ggaggtcgac gcgagccgtc gggcggctcg gatcgaggtg gctcgggcag gggaggcgtt
      841 cgacgagttc ctcaccgagc gggggtcacg aacgctggcg acggtgacct gggggcggat
      901 cgccgccgcc ggggacgacc tcctgctggc ggttgacgcg atggaggtca tgagcctgct
      961 cgggtaccgg accgatggct gcgaggactg cgtcggccgg gtccgccggg acgtggacgc
     1021 ggtggtcggc gtgttcgccg gattcgccga cgagctcgag gcggcccgcc agccacccgg
     1081 cccgcgggtc gaggcggcgg ccgcgacccg ggacgcggtg ttgcagtgcc tccgagcctg
     1141 gggaggtgca cccggctcac cgctcgatcg aaccgccatc ggcctggccg cggcgtcgtt
     1201 ctggagcgtc gagatcgccc gcatcggggg cgagcttgcc gagccgctcc ggtccgtggc
     1261 ggttgccgcc agctcgccgt ggtggcgatg atcgaccgcg atccgcggca ccgacctcag
     1321 gcccccggcc tggcgagtga cgctcgcgcg gtgcgacgcc gtatgcttgg cggcgcggca
     1381 gggggtatcg ccgcctcccg aggccgaaag ggacagcatg gccgatgaag ctcggctcac
     1441 cgccgaggag ctcaacgctg tcgagctcac caaggcgatg cgcggctacg ccatgcacga
     1501 ggtcgacacg ttcctcgacc gggccgtcca ggagctggcc cgcctgcagg ccaagcagag
     1561 cggcgagtac gcgccgtcga cgccgcggct cacccccgag gccatccagc acaaggtctt
     1621 caccaaggcc ctgcgcggct acgccatgca ggaggtcgac gccctcctcg accggctcgc
     1681 cgacgagctc gcccgcctcc agcgcgagga gcgctggatg caggccagcc tgaccggccg
     1741 cccggccgcg atgcccggca tcaccccgga ggagatccag cgcaagcagt tcacggtcgc
     1801 gctgcgcggc tacgtgatgg ccgaggtcga cgacttcctg ctcgaggccg cgcgcgagct
     1861 ggcccgcctg cgggccgagc gcagccgcgg ctaccgcccc tcccccgacc gcctcaccgc
     1921 cgacaacctg cgccactggc gcttcagcaa ggcgatgcgc ggctacgcgg tccacgaggt
     1981 cgaccagttc ctcgaccgcc tggccggcga ggtcggccgc ctgctccgcg acccgacgta
     2041 gggcgcgaag gggcattccc gcagccctgg acagccggcg cgccggcgcc gtgccgcagg
     2101 cggtagcctc gcgccgtcaa gctcgcaaga c
//
LOCUS       DASPYY010000531         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2617940, whole genome shotgun sequence.
ACCESSION   DASPYY010000531 DASPYY010000000
VERSION     DASPYY010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2617940"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2389
                     /locus_tag="VEP73_08455"
     CDS             101..2389
                     /locus_tag="VEP73_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26492.1"
                     /translation="MPHRGVRNSLPHSAWLFVVVVAAAAALAAAAVLPAAVREVSGNP
                     GHLLRPALFALGLVVAERFVVRVPLRNHRFSVGVAEMVIVLGVVFISPPLLVLATGIG
                     IAVSQWCFERQLVKRLFNVPQYMLSVGAAALVAAALIHLLQGSTVFGIRVGSPASFNP
                     TLTILWVVGMATFFLVNHTLVSVVISLSVGHGFAQSWLRAAPVAAADWAASTAYGLVI
                     AALLVHDQALLPLLVVPIGLTFLGNRAWARSLAQGQRMHSLYSAGRALSNRLGDLGAW
                     QTFVRQVAEVLNCEGAAVFLGRPGDGALDVISTDSGSDRLLVTPSPVAWEQAAKGFAT
                     RSRWPRVTMVQMEAEGRTVGLAAAFGPRVDADFTPDDRETLLTLANQGAAALLNEDLY
                     REAESERAALRDIVGHSTDGIYTVGPDRTVRSWNPAMVALTGYAEEEAIGQKCFNLLR
                     ARDGRGVDMCATDCPILAAAESRHEEVRDASVLNKDGLTRWINYAHAPIVGSDDEMDA
                     DVVVVRDVTRERQTDELKSDFVASVSHELRTPLTPIKGFLLTLMRSDRDFAQDRRREY
                     YKLMLTQAQRLERLIEDLLEVTRLETGAGLVDSTPIDAVDLVRQVAERSTAEDPDRAI
                     HIVAPDHAVYARGDWMRVDQVVGNLLSNALRYSPPHEPVEVRLVPQGREVVFEVRDWG
                     PGIPIDEQSRIFERFHRVGHYMTREPGGAGLGLYLAKRLVEAMGGRIWVSSRLGHGSV
                     FSFALPSEPTLGAVGGRGREIG"
BASE COUNT          334 a          966 c          870 g          369 t
ORIGIN      
        1 cagagcccgg gcggcacggt cgcctcgcgg tgccgcccgg agcacacgac gacatgcggg
       61 aacgcagcag gtagcgatgg cttcgatcag cgagggaccc gtgccgcacc ggggagtgcg
      121 caactccctg ccgcacagtg cctggctgtt cgtcgtcgtg gtcgccgccg cggccgcgct
      181 cgcggccgcg gccgtcctgc cggccgccgt ccgggaggtg tccggcaacc ccgggcacct
      241 cctgcgaccc gccctcttcg ccctcggact ggtcgtcgcc gagcggttcg tcgtccgggt
      301 gccgctgcgg aaccaccgat tctcggtcgg cgtggcggag atggtcatcg tgctcggcgt
      361 ggttttcatc agcccaccgt tgcttgtact agcaaccggt atcgggatcg ccgtgagcca
      421 gtggtgcttc gagcgccagc tcgtcaagcg cctgttcaat gttccgcaat atatgttgtc
      481 ggtcggtgcg gccgcgctcg tcgccgccgc gctgatccac ctgctgcagg gctcgaccgt
      541 cttcgggatc cgggtcggct ccccggcctc gttcaacccg accctgacca tcctgtgggt
      601 cgtcgggatg gcaacgttct tcctcgtcaa ccacaccctg gtgagcgtcg tcatcagcct
      661 gtcggtcggt cacggcttcg cgcagagctg gctgcgggcc gcgcccgtgg ccgccgccga
      721 ctgggcggcg agcaccgcgt acggcctggt catcgccgcg ctactggtcc acgaccaggc
      781 cctgctcccc ctgctcgtcg tccccatcgg gctgaccttc ctcggcaacc gggcctgggc
      841 ccgcagcctc gcgcagggcc agcgcatgca ctcgctgtac tcggccgggc gggcgctgtc
      901 caaccggctc ggcgacctcg gcgcctggca gaccttcgtg cggcaggtcg ccgaggtgct
      961 caactgcgag ggcgccgcgg tgttcctcgg ccggccaggg gacggtgccc tcgacgtgat
     1021 ctccaccgac tcgggctccg accggctcct ggtgaccccg tcgccggtgg cctgggagca
     1081 ggcggccaag ggcttcgcca cccgctcccg gtggccgcgg gtcaccatgg tgcagatgga
     1141 ggcggaggga cggaccgtcg ggctggcggc cgccttcggg ccgcgggtcg acgccgactt
     1201 cacccccgac gaccgcgaga ccctgctcac cctcgccaac cagggcgccg ccgccctgct
     1261 caacgaggac ctgtaccggg aggccgagag cgagcgggcc gccctgcgcg acatcgtcgg
     1321 ccactccacc gacggcatct acaccgtcgg gcccgaccgc accgtgcgct cgtggaaccc
     1381 ggccatggtc gcgctcaccg gctacgccga ggaggaggcg atcggccaga agtgcttcaa
     1441 cctgctgcgc gcccgcgacg gcaggggcgt cgacatgtgc gccaccgact gcccgatcct
     1501 ggccgcggcc gagtcccgcc acgaggaggt ccgcgacgcc tcggtgctca acaaggacgg
     1561 cctgacgcgc tggatcaact acgcccacgc gccgatcgtc ggcagcgacg acgagatgga
     1621 cgccgatgtc gtcgtcgtcc gggacgtcac ccgcgagcgc cagaccgacg agctgaagag
     1681 cgacttcgtc gccagcgtct cccacgagct gcgcaccccg ctcacgccga tcaagggctt
     1741 cctgctcacg ctcatgcgct ccgaccgcga cttcgcccag gaccgcaggc gcgagtacta
     1801 caagctcatg ctgacccagg cgcagcggct cgagcgcctc atcgaggacc tgctcgaggt
     1861 gacccgcctc gagaccggcg ccggcctggt cgactccacc ccgatcgacg ccgtcgacct
     1921 ggtccgccag gtcgccgagc gctccaccgc cgaggacccc gaccgcgcca tccacatcgt
     1981 cgcccccgac cacgccgtct acgcgcgcgg cgactggatg cgcgtcgacc aggtcgtcgg
     2041 caacctgctg tcgaacgcgc tgcgctactc gccgccccac gagccggtcg aggtgcggct
     2101 cgtcccgcag ggccgcgagg tggtgttcga ggtgcgcgac tggggcccgg gcatcccgat
     2161 cgacgagcag tcccgcatct tcgagcggtt ccaccgggtg gggcactaca tgaccaggga
     2221 accaggcggc gccggcctcg gcctgtacct ggccaagcgg ctcgtcgagg cgatgggcgg
     2281 gcggatctgg gtgtcgagcc gcctcggcca cggctcggtg ttctcgttcg cgctcccctc
     2341 cgagccgacc ctcggcgcgg tcggcggccg cggccgcgag atcggctgag gcccggcgcc
     2401 cgagcgggaa cccgaggatc tggctacagt gtcgccatga ccgccgacgc gccagccgac
     2461 gactaccgcg ccttcctcga gcggcagcag gtcgcgcggc tgcgcgactg ggcgctcgag
     2521 gtggcccgcg agcaccggg
//
LOCUS       DASPYY010000532         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_933119, whole genome shotgun sequence.
ACCESSION   DASPYY010000532 DASPYY010000000
VERSION     DASPYY010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933119"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2319
                     /locus_tag="VEP73_08460"
     CDS             <1..2319
                     /locus_tag="VEP73_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYT26493.1"
                     /translation="LSPHKGDIQGMVLLLLAALFALGVWAHDGGRAGRFFELLLRGLF
                     GWGAYAVPLLTAWVGVALFRRSSGRDEAVGARVAIGLLALLVGGLGLLHLAKGAPRAS
                     ADPHLLQTSGGLVGAVTATPLAGLLSVWGARAMFVCLVFLGTLVATRTSVASVGRGAA
                     RVGQVLKSWATPPPLPAAPAEPSGAPAKPVTLDDLPGEPAPAPARERRRGRRQAAPPE
                     AAEAAEAAAPPEAAPADDEPVVAAKAPPPPAPLPAELPGAGEATQLAMTLQAEAGGAY
                     TVPPSALLRKARHRPVDDRRLDETAQVLERTLDQFEVDAQVARYTRGPTVTRYEVELG
                     PAVKVARVVGLSHDIAYALASPDVRIIAPIPGKSAIGIEVPNRDRELVTLGDILDTPS
                     LEDDHPLTVALGKDIGGEPVLVNLADMPHVLIAGSTGSGKSVCLNVLVTSLLMRNPPD
                     RVRLILIDPKRVEMTNYEGVPHLITPVVTHPKRAGEALNWAVREMEMRLETLALAGVR
                     NIAAYNKAAVEGTLPTLPSVTLDDEGRPDADAPRPTLPYVVVVIDELSDLMMVAPRDV
                     EDAICRIAQMARAVGIHLVVATQRPSVDVVTGLIKANIPSRIAFMVASAQDSRVILDA
                     GGADKLVGHGDMLYLPGGTSKPRRIQGAFITEKEVEAVVAYCKAQRQAAYQPGVVAEG
                     RSASDDDGDEDPLLTQAMEIIVRSGLGSTSMLQSKMKVGFSRARRIMDQLEDRGVVGP
                     SEGSKPRDVLMTVEELGELRGREEGYGEIAGE"
BASE COUNT          337 a         1019 c          926 g          343 t
ORIGIN      
        1 ctctcgcccc acaagggcga catccagggc atggtgctgc tgctcctggc ggcgctgttc
       61 gccctcgggg tgtgggccca cgacggcggc cgtgccggcc gcttcttcga gctcctcctc
      121 cgcgggctgt tcggctgggg cgcctacgcg gtgccgctgc tcaccgcctg ggtcggcgtc
      181 gcgctgttcc ggcgctcgtc cggcagggac gaggcggtcg gcgcccgggt cgccatcggg
      241 ctgctcgcac tgctggtggg cggcctcggc ctgctccatc tggccaaggg cgccccgcgg
      301 gcgtcggccg acccccacct gctgcagacc tcgggcggcc tggtcggcgc ggtcaccgcc
      361 acgcccctgg ccggcctgct gtcggtctgg ggcgccaggg ccatgttcgt ctgcctggtg
      421 ttcctcggca cgctcgtcgc caccaggacc tcggtggcgt ccgtcgggcg cggcgccgcc
      481 cgcgtcggcc aggtcctcaa gtcctgggcg acgccacccc cgctcccggc cgccccggcc
      541 gagccgtccg gcgcgccggc gaagcccgtc acgctcgacg acctgcccgg cgagcccgcg
      601 ccggctcccg ccagggaacg gcgccggggc cgccgccagg ccgcgccccc cgaggccgcc
      661 gaggccgccg aggccgcagc gccgcccgag gccgccccgg ccgacgacga gccggtcgtg
      721 gcggccaagg ccccgccccc gcccgcgccg ctccccgccg agctgccagg ggcgggcgag
      781 gcgacccagc tcgccatgac cctgcaggcc gaggcgggcg gggcctacac cgtcccgccc
      841 tcggccctgc tccgcaaggc gcggcaccgc ccggtcgacg accgcaggct ggacgagacg
      901 gcgcaggtgc tcgagcgcac cctcgaccag ttcgaggtgg acgcccaggt cgcccgctac
      961 acccgcgggc ccaccgtcac ccgctacgag gtcgagctcg gcccggccgt caaggtcgcg
     1021 cgggtggtcg ggctgtcgca cgacatcgcc tacgcgctgg catcgcccga cgtgcgcatc
     1081 atcgccccga tcccgggcaa gtcggcgatc ggcatcgagg tgccgaaccg cgaccgcgag
     1141 ctcgtcaccc tgggcgacat cctcgacacg ccgtccctgg aggacgacca cccgctgacg
     1201 gtcgccctcg gcaaggacat cggcggcgag ccggtcctcg tcaacctggc cgacatgccg
     1261 cacgtgctga tcgcggggtc gaccgggtcg ggcaagtcgg tctgcctcaa cgtgctggtg
     1321 acgagcctgc tgatgcgcaa cccccccgac cgggtccgcc tgatcctgat cgaccccaag
     1381 cgcgtcgaga tgaccaacta cgagggcgtg ccccacctga tcacgccggt ggtgacccac
     1441 cccaagcgtg ccggcgaggc gctcaactgg gccgtgcggg agatggagat gcgcctcgag
     1501 acgctcgcgc tcgccggcgt gcgcaacatc gccgcctaca acaaggcggc cgtcgagggg
     1561 acgctgccaa cgctgccctc ggtcaccctg gacgacgagg gccgcccgga cgccgacgcg
     1621 ccccggccga ccctgcccta cgtggtggtc gtgatcgacg agctctccga cctcatgatg
     1681 gtcgcgcccc gcgacgtcga ggacgcgatc tgccgcatcg cgcagatggc ccgggcggtc
     1741 gggatccacc tggtggtggc cacccagcgg ccctcggtcg acgtggtcac cgggctcatc
     1801 aaggccaaca tcccgagccg gatcgcgttc atggtcgcga gcgcccagga ctcccgggtc
     1861 atcctggacg ccggcggcgc cgacaagctc gtcggccacg gcgacatgct ctacctccct
     1921 ggcggcacct ccaagccgcg ccggatccag ggcgcgttca tcaccgagaa ggaggtggag
     1981 gcggtcgtcg cctactgcaa ggcgcagcgc caggccgcct accagcccgg ggtggtcgcc
     2041 gagggccggt cggcgagcga cgacgacggc gacgaggacc cgctcctcac ccaggcgatg
     2101 gagatcatcg tgcggtcagg gctcgggtcg acctcgatgc tccagtccaa gatgaaggtc
     2161 ggcttctccc gcgcgcgccg catcatggac cagctcgagg accgcggggt cgtcggcccg
     2221 tccgagggat ccaagccccg cgacgtgctc atgaccgtgg aggagctcgg ggagctccgg
     2281 ggccgcgagg agggctacgg cgagatcgcc ggcgagtagc cggcggtgaa cggccccccc
     2341 agtccggtgt cggtccggtg acacccgctc gcgaccgttg cccggggggt tggcgcttct
     2401 ctacaatcac tgcctgctcg accgattttc tggcttgccc cgctcgtacc gctggggggc
     2461 cactccaagc tcgcagaggg gtgtcttggc ctgaacacca tcggcgacgc gctgcgctcg
     2521 gcccgggagg cgcaggggaa gtccatccag gaggtctccg ccgccaccca catccggtcg
     2581 agccacctcg aggcgctcga ggaggagcgg ttctccgcgc tcggc
//
LOCUS       DASPYY010000533         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2197832, whole genome shotgun sequence.
ACCESSION   DASPYY010000533 DASPYY010000000
VERSION     DASPYY010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197832"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VEP73_08465"
     CDS             complement(<1..1630)
                     /locus_tag="VEP73_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYT26494.1"
                     /translation="MGDGTTPWLDSYPPGVPEHVEVPATNLARVLDDAARDFPNAPAL
                     HFEGRTISYAQLADRAWRFAGVLAGLGVTKGTRVGLVLPNCPQAVIAFFGALRLGAVV
                     VQNNPLYTERELGHQLTDAGVEVLVCLDLAYERVKAARAQTSIREVVVTSVLDELPAV
                     KRVLAPYTRKGREAVAAVGRDEPVRRWRDLSSGAAKPPEAAVDTASDLALLQYTGGTT
                     GLSKGVMLSHGNLRANVEQVRAWFPDADPGREVMMAVLPFFHVYGLTVCLLLGVRVGA
                     ALVLMPRFDLDAVLAAVDRYRPTLFPGVPTMYVALNNAVAKGGHDLSSIKACLSGAAP
                     LPLEVAERFERYSGGRLVEGYGLSECSPVALANPIYGKRKAGTIGMPLPDTIARVVDP
                     SEPDRALPSGERGELAIRGPQVMLGYRNRPEESAEVLRDGWLLTGDMAVMDEEGYFTI
                     VDRKKDLIIAGGYNIYPREVEEVLYEHPKVLEVCVAGVPDPYHGETVKAFVVLRAGEQ
                     ATQDEIREFAKERLTGYKVPRAVEFRDELPKTLIG"
     gene            complement(1722..2987)
                     /locus_tag="VEP73_08470"
     CDS             complement(1722..2987)
                     /locus_tag="VEP73_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26495.1"
                     /translation="MGGRQPEGGRARQPEGRRGRQAEGRHARQAEGRHDPDDTLPWLA
                     ALDEDPEEDAEWARKLRPRQAPRVDMPGPEPPGLADDEEPERSEFSSRQQWRPAEPSY
                     DALGDDLGDQTGSWSFDDEPATRPPYPDATAAWQPESRHRADPPPPRPAWELPAPPRA
                     EAAASFWEPSAPAWEPEPHAGEEAGPAPWEAGPAPAAEYDFGSLFAEQAPTQSLPRES
                     LPRESLPRESLPRESLPPESSWRWEDEREQADLLSGGEPVVPFGPPSGRALAPATPAG
                     PARPPHPAAPGRALEPPAAGREHDASGRDASDQATQAWARGATGDGEWPRPAGDDSRA
                     RMPGGRRVRAPEDGHVVGRGEAVAPIEGRHMRQLEARPDGRRARQRPARVRRRWPVRL
                     AILAWIVLFAVICWLYVFPWLEGVLPENF"
     gene            complement(3005..3652)
                     /locus_tag="VEP73_08475"
     CDS             complement(3005..3652)
                     /locus_tag="VEP73_08475"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HYT26496.1"
                     /translation="MRRLLRGTGWTFIAMGCFVLYFLTYQLVGTNASANQQQGSLRKQ
                     LESTWTAQAGIPAGRSSVAKPRGLPLGAAVAIIQIPRIKVERVIVQGVGRADLKKGPG
                     HVPSTVMPGQAGTFGVSGHRTTYGAPFFRLDELTRGDDIVIVTKASVFTYHVTGKRVV
                     PPTDTAVLDNVQGAGGRLKATITLTTCNPRFSAKQRLVVFGDLAATAPNNGTVAA"
     gene            complement(3694..4443)
                     /locus_tag="VEP73_08480"
     CDS             complement(3694..4443)
                     /locus_tag="VEP73_08480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYT26497.1"
                     /translation="MPGRPAGPRARERMTCRDRTVPARRPPGRAVAARGALLALCLLA
                     AACTAGARPAPAPPEALGSRQLVDRTADQFQRDLDRLKGRVVVVNFWASWCAPCGAEM
                     PALERASRDLAGKPVSFVGVDASDERSRAAAVLARAGVTYPTVYDRKGIYGGLASRWS
                     VTSLPQTWLVDTDGRRAIRIARQVQPGELRAAVDRLLAGSRGGAPEPASAASPTGGGE
                     RAPADPCATAISRHRQGVRPPGASVLHTRTA"
BASE COUNT          554 a         1693 c         1671 g          598 t
ORIGIN      
        1 tgccgatcaa cgtcttgggc agctcgtcgc ggaactcgac cgccctgggc accttgtagc
       61 cggtgagccg ctccttcgcg aactcgcgga tctcgtcctg ggtggcctgc tcgccggcgc
      121 gcagcacgac gaacgccttc accgtctcac cgtggtaggg atcgggcacg ccggcgacgc
      181 agacctcgag caccttcggg tgctcgtaga gcacctcctc gacctcgcgg gggtagatgt
      241 tgtacccgcc cgcgatgatc aggtccttct tgcggtcgac gatcgtgaag tagccctcct
      301 cgtccatcac tgccatgtcg ccggtgagca gccatccgtc ccggaggacc tcggcggact
      361 cctccggccg gttccggtag ccgagcatca cctgcgggcc gcggatggcc agctcccccc
      421 gctcgccgct cggcagggcc cggtcgggct cggacgggtc aacgacccgc gcgatggtgt
      481 cgggcagggg catgccgatc gtgcccgcct tgcgcttgcc gtagatcggg ttggcgagcg
      541 ccaccgggga gcactccgag aggccgtagc cctcgacgag cctgccgccc gagtagcgct
      601 cgaagcgctc ggccacctcg agcggcaacg gcgccgcgcc ggacaggcac gccttgatgc
      661 tggagaggtc gtgcccgccc ttggccacgg cgttgttgag cgccacgtac atggtgggca
      721 cgccaggaaa cagggtgggc cggtacctgt cgacggcggc gagcacggcg tcgaggtcga
      781 acctgggcat gagaaccagg gcagccccga cccgcacgcc gaggagcagg cagacggtga
      841 gcccgtagac gtggaagaag ggcaggaccg ccatcatcac ctcgcgccca gggtcggcgt
      901 cggggaacca ggcccgcacc tgctcgacgt tggcccgcag gttgccgtgg gagagcatga
      961 cgcccttgga gaggccggtg gtgccgcccg tgtactgcag gagcgcgagg tcggaggccg
     1021 tgtccaccgc tgcctcgggc ggcttggcgg caccggagga gaggtcgcgc cagcggcgca
     1081 ccggctcgtc cctcccgacc gccgcaacgg cctccctgcc cttcctggtg tagggcgcga
     1141 gcacgcgctt gaccgcgggc agctcgtcga gcaccgaggt cacgaccacc tcgcggatgg
     1201 aggtctgcgc gcgcgcggcc ttgacccgct cgtaggcgag gtcgaggcag acgagcacct
     1261 cgaccccggc atcggtgagc tggtggccga gctcgcgctc ggtgtacagc gggttgttct
     1321 gcacgacgac ggcgcccagc cgcagcgcgc cgaagaacgc gatcaccgcc tgcgggcagt
     1381 tcggcaggac gagcccgacc cgggtgccct tggtcacccc gaggccggcc agcacgcccg
     1441 cgaaccgcca cgcccggtcg gcgagctgcg cgtaggagat ggtgcggccc tcgaagtgga
     1501 gcgccggcgc gttcgggaag tcgcgggcgg cgtcgtcgag cacgcgtgcc aggttcgtcg
     1561 cgggcacctc gacgtgctcg ggcacgccgg gcgggtagct gtcgagccag ggtgtcgtgc
     1621 cgtcgcccat cgttggtctt ccctccaagc ccgcccgtcc ccggtcggca gtatccgtcg
     1681 tcctcggccg tcacgacaat ctccggccgc cgctgcctcg gtcagaagtt ctccggcagg
     1741 actccctcga gccagggaaa gacgtacagc cagcagatga ccgcgaacag cacgatccat
     1801 gccaggatgg cgagccgcac cggccatcgg cgccgcaccc gggcgggccg ctggcgtgcc
     1861 cgccggccat cggggcgagc ctcgagctgg cgcatgtgcc ggccctcgat cggcgcgacc
     1921 gcctcccccc ggcccaccac gtggccgtcc tccggcgcac gcacgcgccg ccctcctggc
     1981 atgcgcgccc ggctgtcgtc gccggccggc cggggccact ccccgtcccc ggtcgccccg
     2041 cgcgcccagg cctgcgtggc ctggtcgctc gcgtcccgcc ccgaggcgtc gtgctcccgg
     2101 cccgctgcgg ggggctcgag cgcgcggccc ggcgccgccg ggtgcggcgg gcgcgccggc
     2161 ccggcgggcg tggccggcgc cagcgcacgg ccggacggcg gtccgaacgg gaccaccggc
     2221 tcgccgccgg agagcaggtc ggcctgctcg cgctcgtcct cccaccgcca cgaagactcc
     2281 ggcgggaggc tctcccgggg gaggctctcc cgggggaggc tctcccgcgg gaggctctcc
     2341 cgcgggaggc tctgggtcgg cgcctgctcg gcgaagaggg agccgaagtc gtactcggcg
     2401 gccggcgccg gcccggcctc ccagggcgcc gggccggcct cctcgcccgc gtggggctcg
     2461 ggctcccagg ccggagcgct cggctcccag aagctcgcgg cggcctccgc cctgggcggc
     2521 gctggcagct cccaggccgg cctgggcggc ggcgggtcgg cccggtgccg ggactcgggc
     2581 tgccacgcgg cggtggcgtc ggggtacggc ggccgggtgg ccggctcgtc gtcaaagctc
     2641 cagctcccgg tctggtcccc gaggtcgtcc ccgagcgcgt cgtaggacgg ctcggccgga
     2701 cgccactgct gcctggagga gaactcggag cgctcgggct cctcgtcgtc agccaggccg
     2761 ggcggctccg ggccgggcat gtccaccctc ggggcctgcc tgggacgcag cttgcgcgcc
     2821 cactcggcgt cctcctcggg gtcctcgtcg agagcggcga gccaagggag ggtgtcgtcg
     2881 gggtcgtgcc tgccctcggc ctgccgggcg tgcctgccct cggcctggcg tccgcggcgg
     2941 ccttccggct ggcgggcacg cccgccctcg ggctggcgcc cccccaagtc ctcaggccac
     3001 cggctcatgc cgctactgta ccgttgttgg gcgcggtggc cgcaaggtcc ccgaagacga
     3061 ccaagcgctg tttcgcggag aagcgcgggt tgcaggtggt cagcgtgatg gtcgccttga
     3121 gcctgccgcc ggccccctgg acgttgtcga gcaccgccgt gtccgtcggc ggcacgaccc
     3181 gcttcccggt cacgtggtag gtgaacacgc tcgccttggt cacgatcacg atgtcgtcgc
     3241 ccctggtcag ctcgtcgagc cggaagaagg gcgcgccgta ggtggtgcgg tggcccgaca
     3301 cgccgaacgt gccggcctgg ccgggcatca ccgtgctcgg cacgtggccc gggcccttct
     3361 tcaggtcggc gcggccgacg ccctgcacga tgacccgctc gaccttgatc ctggggatct
     3421 ggatgatggc gacggccgcg cccagcggga ggcccctcgg cttggccacg ctcgatcggc
     3481 cggccgggat gccggcctgg gcggtccagg tcgactcgag ctgcttgcgg aggctcccct
     3541 gctgctggtt cgcggacgcg ttggtgccca cgagctgata cgtcaggaag tagaggacga
     3601 agcaccccat cgcgatgaac gtccagccgg tcccgcgcag caacctccgc atcgtcacct
     3661 ccgagccatc accgccacga tggccatgca atcctacgcg gtcctggtgt gaaggacgga
     3721 ggcccctggt ggccgcacgc cctgccggtg gcgggagatg gcggtggcac acgggtccgc
     3781 cggcgcccgt tccccgccgc ccgtcggcga cgcggcgctc gccggctcgg gcgcaccccc
     3841 gcgcgagccg gcgagcagcc ggtccaccgc ggcccgcagc tctccgggct ggacctgcct
     3901 ggcgatccgg atggcgcgcc gtccgtcggt gtccacgagc cacgtctgcg gcagggaggt
     3961 caccgaccat cgcgaggcca gcccgccgta gatgcccttc cggtcgtaga cggtggggta
     4021 ggtgacaccg gcccgggcca ggaccgcggc cgcccgggac cgctcgtccg aggcgtcgac
     4081 cccgacgaag ctcaccggct tcccggcgag gtcccggctg gcgcgctcga gggccggcat
     4141 ctcggcaccg cacggggcgc accagctcgc ccagaagttg accaccacga cgcggccctt
     4201 gagccggtcg aggtcgcgct ggaactggtc cgcggtccgg tcgacgagct gccgcgagcc
     4261 gagggcctcg ggtggggccg gcgcgggcct ggcaccggcc gtgcaggcgg ccgccagcag
     4321 gcagagggcc agcagggccc cgcgcgcggc gaccgcgcgc cccggcggcc gccgcgccgg
     4381 gacggtacga tcgcggcagg tcatgcgctc gcgcgcccga gggccggcag gccggcccgg
     4441 cacggggcgc atcctccgaa gggaccttgc ccgaaaggac cttgcccgaa aggaccttgc
     4501 ccgaaaggac cttgcc
//
LOCUS       DASPYY010000534         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2479032, whole genome shotgun sequence.
ACCESSION   DASPYY010000534 DASPYY010000000
VERSION     DASPYY010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479032"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..>1567
                     /locus_tag="VEP73_08485"
     CDS             323..>1567
                     /locus_tag="VEP73_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYT26498.1"
                     /translation="MPSSLSSLLADTDRLTAVRQTNLLDTPGEEAFDRLVRLAARALR
                     APIALMSLVAEDRQFFKACIGLPESWASARQTPVSHSLCQHLVATGQPLVIGDVRRDR
                     RAHDNPLVAELGVAAYLGVPLVSSSGHVLGSFCVADRRPRAWTADDKAVLADLAGVAM
                     DEIRLRTDVGARRSDAAGSGTHPAATEQEAASRRELQGRLADVEASLRALLERLPGVV
                     YTISPEAPNPPTYISPQIETLLGEPAAACVGDPGAWDRWIDPDDADWVLAECERTNRT
                     GEPFAAEYRMRTRDGEVRWVRDEAVLVRGGDGRPLFWQGILTDVTATHLTAARLAEAL
                     ERERAAAEHLRAVDEMKTTFLQAVSHELRTPLTTILGAALTLERGAAGLPAHLVADLL
                     HRLSSNARRLDRLLADLLDLDRL"
BASE COUNT          215 a          584 c          568 g          200 t
ORIGIN      
        1 gccgtggtgg tggcgtcggt ggacccgtcg tagcgcaggt agctcgaggg cgtgccgtcg
       61 cgggtgaccc ggacgtcggc accgctgccg agcgccgcgc cgctgaaggc gaccgcaagc
      121 accagggcca ggggaacgag cagccagcga gatctcatga ggacctcctc atccggccgg
      181 cggtccatct ccctcgttgg gcagacgccg gccggatgag gctcgcaaac ccgcaggttc
      241 tggtcaaccc cgcgacgggg ggtgacgtag agtagcggtc cccacttgag gtcggccctg
      301 aaggcgaagg gagtcccggc cggtgccctc cagcctgagc agcctgctgg ccgacacgga
      361 tcggctgaca gccgtgcgcc agacgaacct gctcgacacg cctggcgagg aggccttcga
      421 ccggctggtc cggctggccg cccgtgcgct gcgagcgccg atcgccctga tgtcgctggt
      481 cgccgaggac cgccagttct tcaaagcatg catcggccta cccgagtcct gggcgtcggc
      541 caggcagacg cccgtctctc actcgctgtg ccagcacctg gtcgcgaccg ggcagccact
      601 ggtgatcggc gatgtgcgtc gcgaccggcg tgcccatgac aatccgctgg tcgccgagct
      661 gggcgtggcc gcctacctcg gcgtcccgct ggtcagctcg tcggggcacg tgctggggtc
      721 gttctgcgtc gccgaccggc gcccgcgggc ctggaccgcc gacgacaagg ccgtcctcgc
      781 cgacctggcc ggcgtggcga tggacgagat ccggctgcgg accgacgttg gcgcgcgccg
      841 gtcggacgcc gccgggagcg gcacccaccc ggcggccacc gagcaggagg ccgcctcgcg
      901 ccgcgagctc caaggacggc tggccgacgt cgaggcgagc ctgcgggcgc tgctggaacg
      961 cctgcccggg gtcgtgtaca cgatcagccc cgaggccccc aacccgccga cctacatcag
     1021 cccgcagatc gagacgctgc tcggcgagcc ggcggcagcc tgcgtcggcg accccggcgc
     1081 gtgggaccgc tggatcgacc cggacgacgc cgactgggtg ctggccgagt gcgagcggac
     1141 caaccggacc ggggagccgt tcgcggccga gtaccggatg cgaaccaggg acggcgaggt
     1201 gcgctgggtc cgcgacgagg cggtcctggt ccgtggtggc gatggccggc cgctgttctg
     1261 gcagggcatc ctgaccgacg tcaccgccac ccacctgacc gccgcccggc tcgccgaagc
     1321 gctggagcgc gaacgagccg cggccgagca cctgcgggcc gtggacgaga tgaagacgac
     1381 cttcctgcag gcggtctcgc acgagctgcg cacgccgctc accaccatcc tcggcgccgc
     1441 gctcacgctg gagcgcggcg ccgcgggcct gcccgcccac ctcgtcgccg acctgctgca
     1501 ccggctgtcc agcaatgccc gccggctcga ccggctgctc gccgacctgc tcgacctcga
     1561 ccggctc
//
LOCUS       DASPYY010000535         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1497056, whole genome shotgun sequence.
ACCESSION   DASPYY010000535 DASPYY010000000
VERSION     DASPYY010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497056"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VEP73_08490"
     CDS             <1..221
                     /locus_tag="VEP73_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26499.1"
                     /translation="ELARRNGTEALAGLPSDPDKLRAEWDAASVDRRRAIVRAVLDSV
                     IIGPYPGPAKGRWGNVFDPDRVEIRWKE"
     gene            528..785
                     /locus_tag="VEP73_08495"
     CDS             528..785
                     /locus_tag="VEP73_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26500.1"
                     /translation="MFIATLVLVRALIAAAGRDGQHEFMSIGEVAELTRKSLNALYSM
                     RYTGTGPPSFRLGRRLVYRRTDVLAWIERHAAADKHSGVAR"
     gene            782..1003
                     /locus_tag="VEP73_08500"
     CDS             782..1003
                     /locus_tag="VEP73_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26501.1"
                     /translation="MTREQLAARVARVAADRQRQGFPAHVEDEATLAKVAALLAEPGP
                     ARDDGGPAEPAADPEGATRRPDRSRHGGA"
     gene            990..1532
                     /locus_tag="VEP73_08505"
     CDS             990..1532
                     /locus_tag="VEP73_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26502.1"
                     /translation="MAEHSASTRQPLPGSPGCACCTPDPARRAALGRLRAEHERLRIT
                     AWAHSLMPLDRHYGPRWAAMTGEPPRRSRVRPGLTPGRPRRLVETPAYFGMVRRVLRA
                     AARRAGAGDLDALADLAALRGELDGHLVDAVAVLRHEPWSYSWGEIGAALGVSRQTAQ
                     ERFRKAGGSRRPGGQPGSLR"
BASE COUNT          227 a          658 c          596 g          207 t
ORIGIN      
        1 cggagctggc ccgccggaac ggcaccgagg cgctcgccgg gctgccgagc gacccggaca
       61 agctccgagc cgagtgggac gccgcctcgg tggaccgccg ccgcgcgatc gtgcgcgcgg
      121 tcctggactc ggtgatcatc ggcccgtacc ccgggccggc gaagggccgc tgggggaacg
      181 tgttcgaccc ggaccgggtc gagatccgct ggaaggagta gccttacgct cccccaatta
      241 cgctcccccg ggtactaggc tcgccgcgta attgacctgc gatgatgctt gacgaacagc
      301 cacaaatgag gtacttctaa acgcggcccg ccccggcggg cccactcggg aggatcgaga
      361 cgcgtcctgt gcgagggggg gagggggctc cctgtcgctg cgtttggtcc accggacagg
      421 gggagcccct gatgacgaag ggcacgtgag caccccgtgc ccaccggtgg ccaaagcggc
      481 tcgctagcag gccgaagagt tccgcctgct ccgtgaggag ccgcaccatg ttcatcgcaa
      541 cgctcgtgct cgtgcgcgcg ctgatcgccg ccgccggccg ggacggccag cacgagttca
      601 tgtccatcgg cgaggtcgcc gagctgaccc gcaagtcgct gaacgccctc tacagcatgc
      661 ggtacacggg caccgggccg ccgtcgttcc ggctcggccg ccgcctcgtc tatcgccgca
      721 ctgacgtcct cgcctggatc gagcgccacg ccgccgcgga caagcactcg ggggtcgccc
      781 ggtgacccgc gagcaactgg ccgcccgggt cgcccgggtc gccgccgacc gccagcgcca
      841 aggcttcccc gcccacgtcg aggatgaggc cacgctcgcc aaggtcgccg cgctgctcgc
      901 cgagcccggc cccgcacgcg acgacggcgg gcccgctgag cccgccgccg atcccgaagg
      961 cgccacccga cgtcccgacc ggagtcgcca tggcggagca tagcgcgtcg actcgacagc
     1021 cgttgcccgg gtcgcccggc tgcgcgtgct gcacgcccga cccggcccgc cgcgccgcgc
     1081 tcggccggct ccgcgccgag cacgagcggc tccggatcac cgcgtgggcg cactcgttga
     1141 tgccgctcga ccggcactac gggccaaggt gggcggccat gaccggcgag cctccccgcc
     1201 gttctcgcgt caggcccggc ctgacgccag gacggccccg gcggctggtt gaaacccctg
     1261 cgtacttcgg gatggtgcgg cgcgtcctcc gggccgccgc ccgccgcgcc ggtgctggcg
     1321 acctcgacgc cctcgccgac ctcgccgcgc tccgcggcga gctggacggc cacctggtcg
     1381 acgcggtcgc ggtgctccgc cacgagccgt ggtcgtactc ctggggtgag atcggcgccg
     1441 cgctcggggt gagcaggcag acggcgcagg agcggttccg caaggccggc gggtcccgcc
     1501 ggcccggcgg ccagccgggg agcctccggt gaccggcaag gcgttcgaca tggccgacca
     1561 ggcagcggcg tccgcgctgg aaggggctgc ctctacccca cccaacattg cgggtacacc
     1621 ctcaccgcct cccgcggaac agccgacgtc cgccggccac cccagccgcc caggctgccg
     1681 caggccgt
//
LOCUS       DASPYY010000536         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_89425, whole genome shotgun sequence.
ACCESSION   DASPYY010000536 DASPYY010000000
VERSION     DASPYY010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89425"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VEP73_08510"
     CDS             <1..659
                     /locus_tag="VEP73_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HYT26503.1"
                     /translation="PVVDARYTYLGVLAALGAALARWSLRARGDLLLGAAVVLNPILL
                     AFTRWGTNDVLPMAGLALRGWALSRRRTVLAGLGLGLALACKLLAAPAAALFLVWLAR
                     RVADRELGRRAAVGAGAATVLPALVSAAPFLLWHPAAFLDDAVRYHLGAGRNPFPVAG
                     QGLPALLYALRVAHTRSARCRCGPPRSRPWPPWRSAPAGCGAPAGGTAGRRGVPTPLG
                     "
     gene            838..1104
                     /gene="rpsO"
                     /locus_tag="VEP73_08515"
     CDS             838..1104
                     /gene="rpsO"
                     /locus_tag="VEP73_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYT26504.1"
                     /translation="MLVKDEKTEIITQYATKDGDTGSPEVQVAILTRRINTLTEHLKV
                     HKHDHHSRRGLLQMVGRRRRLLRYLQTNDVARYRSLIERLGLRR"
     gene            1462..3693
                     /locus_tag="VEP73_08520"
     CDS             1462..3693
                     /locus_tag="VEP73_08520"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYT26505.1"
                     /translation="MAVHRVQGSVGGRDMFLETGKLAGQAGGAVVIGLGETELLVTAT
                     AGKQPKEQLDFFPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTARLVDRPLRPSF
                     PKGLRNEVHVVATILSADMENPPDILAINGASAAVLLAGIPFSGPVGAVRLGRTGTDW
                     IVNPTYSELAERTFDMVVAGRQNEAGGVDILMVEAEATEHAVRLMEEDGAPKPTEEAL
                     GLALELSKSHIATACQLQLELVRQAGAQPREFPLFAEYGEDVAGAVRGAVEAATREAV
                     AIPDKTERQNRLDELKAEAQQAVSGQLGDGLTGREKEIAAAVRSLTKELVRQRVVKEG
                     RRIDGRGPRDIRPLQAEVGILPRAHGSGLFQRGETQVLNVTTLGMVRMEQTVDTLDPE
                     DRKRYMHHYNFPPFSTGEVGFMRGPKRREIGHGALAERALLPVIPDKDEFPYALRLVS
                     EVLSSNGSTSMASVCASTLSLMDAGVPIKAPVGGIAMGLIAEGGEYVTLTDILGAEDA
                     FGDMDFKVAGTREFVTALQLDTKLTGIPSEVLIAALTQARDARLVVLDVIQAAIERPR
                     GELNRLAPRVHVEQIPVAKIGELIGPKGKRINEIQAASGADISIEDDGRVFIGATDGE
                     QLDIALRMIREQMNPVMPQAGERYLGTVVKTTTFGAFISLTPGKDGLLHISKLGRKGQ
                     RVRAVEDVVNVGDKLLVEVVEVDRQNRISLRLAEEGEAAPRDGDDAAPVAAGDGTPDD
                     ESQ"
     gene            3700..4974
                     /locus_tag="VEP73_08525"
     CDS             3700..4974
                     /locus_tag="VEP73_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYT26506.1"
                     /translation="MPRSGAFERTELAGGLRILTERMPSVRSVTLGIWAGVGSRDETP
                     RLAGASHYLEHLLFKGTSRRSAQEIAEVMDAVGGEMNAFTFKEYTCFYARTLDRDLPL
                     AVDVLVDMVRDSKLAKADVEAERTVILEEIGAANDVPEDLVHDLFAEVLFGGHPLGRP
                     VLGTAASIGAMPRDAIARYWRRHYTPGNLVVAAAGSCRHDEVVELLAEAFGDLGAGAP
                     AGLRGERKEPRFAGGAYVRRKPTDQAHVVLGCPGVSRSDPRRFALGVLNIAFGGGMSS
                     RLFQEVREKRGLVYSIGSYTGQYSETGSFAVYAGAAPRRIHEVLRVVREETERVVADG
                     LTDEELERSKGHFKGSLVLGLEDTSSRMSWLGKSELTRGEILSVDEVIRRVDAVTMAD
                     VRAVAKEVLGGGPRALALIGPFDPDGFEEYVS"
     gene            5109..5711
                     /locus_tag="VEP73_08530"
     CDS             5109..5711
                     /locus_tag="VEP73_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYT26507.1"
                     /translation="MFCASARGLPEGYHRAARRLGRELARRGHRLVYGGGDAGLMGEV
                     ARAVHEHGGRVFGAIPRSLVERELAYGPADELVVTETLRQRKAEMDAHADAFVALPGG
                     YGTLEELLEVLTLRQLRLHDRPIVLLNTDGYYDPFLAMVRGMVDQGFVPAGDGRLFQV
                     AAAPEEALDLVEAGVGRRAPPGGPPAEALAEDWTAELPAS"
     gene            complement(5737..6765)
                     /locus_tag="VEP73_08535"
     CDS             complement(5737..6765)
                     /locus_tag="VEP73_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYT26508.1"
                     /translation="MAGRRTNLALLGSLVVALATGGLAFALGTPAGRLVVVAHGAAGL
                     ALVLLVPWKSAVARRGLARRRPGRGASVPLAVLVAAAVASGTLHAMGVGAVGPVTAMQ
                     LHVGAALAAVPLLAWHATARRVRPRRTDLSRRNALRAGALACGAGLAWAGLEGVARVA
                     GLPGAGRRFTGSLRVESADPDHIPVTQWLDDRVPAIDPAGWRLEVRAGSRRRTLTYGE
                     LGMLADGEPGRRTDAVEAALDCTGGWYATARWEGVRLDRLLAGMGEGQSILVTSRTGY
                     QRRLPFAAAGSLLLATRVAGRPLSAGHGFPARLVVPGRRGFWWVKWVTSIEVDATPAW
                     RQPPFPLS"
     gene            complement(6768..>7026)
                     /locus_tag="VEP73_08540"
     CDS             complement(6768..>7026)
                     /locus_tag="VEP73_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26509.1"
                     /translation="RGLARAVVEAAARAVLAEGRAVLYNHAEDNYASAKVAEAAGLHE
                     FGRYHAVVPDRRLGAEFGEDDTAAERTAVRLKAASRGTTER"
BASE COUNT          926 a         2611 c         2630 g          859 t
ORIGIN      
        1 tgcccgtggt cgacgcccgc tacacctacc tcggcgtgct ggccgcgctc ggcgccgcgc
       61 tcgcccgctg gagcctgcgg gcccggggcg acctgctgct gggcgccgcg gtcgttctca
      121 acccgatcct cctcgccttc acccgctggg gcaccaacga cgtcctgccc atggccgggc
      181 tcgcgctcag gggctgggcg ctgtcgcggc ggcgcaccgt gctcgccggg ctcgggcttg
      241 ggctcgcgct cgcctgcaag ctgctggcgg cgccggccgc ggcgctgttc ctggtctggc
      301 tcgcccgccg ggtggccgac cgggagctcg ggcgccgggc ggcggtaggg gccggggccg
      361 cgaccgtgct cccggccctc gtcagcgcgg cgccgttcct gctctggcac ccggcggcct
      421 tcctcgacga cgcggtgcgc taccacctcg gcgccgggcg caacccgttc cccgtggccg
      481 ggcagggact gccggcgctg ctgtacgccc tccgggtggc ccacacccgt tcggcccggt
      541 gccgctgtgg gccaccgcgg tcccggccct ggccgccctg gcgctcggca ccggctggct
      601 gtggcgcgcc ggccggcggt accgccggcc ggcgcggggt gccgacgccc ctcggctgac
      661 ggcgacggct gcggcgggtg acggcgcagc tcacggtgct cgtgctaccc ttgcgcaagg
      721 cggccgggcc ctggtcgggc ctggcggccg cccgggacgc gggactgcgc agcgccgttc
      781 ggcctgctgc gaggcccccg ggcagacttc cgcagcgttc acccgaaagg tagcggcatg
      841 ctggtcaagg acgagaagac ggagatcatc acccagtacg cgaccaagga cggcgacacc
      901 gggtcgccgg aggtccaggt cgcgatcctc acccggcgga tcaacacgct caccgagcac
      961 ctgaaggtcc acaagcacga ccaccactcg cgccgtgggc tcctgcagat ggtcggccgg
     1021 cgccgccggc tcctccgcta cctgcagacc aacgacgtcg cccggtaccg ctcgctgatc
     1081 gagcgcctgg gcctgcgccg ctgaccgggc cgcgcccgtg ccagcacgcg acggcgccgc
     1141 agcgcggcgc catcgctccc ggaaggtggt gacgccgctg gtggactagt gccggaccac
     1201 gacggacctc gcggcacggg tggccagttg tcagtggtga gccctggatc gggcgccgac
     1261 cgctccaggc ttctctactg agaactggca ccgcgccagc cgcggccccg ggccccgcgc
     1321 gcccaccgcg cggccgcgag ccgcccgccc ggccgtcacc gccgcccgcc ggggcctgcc
     1381 aaacggcagc gcccggccgg ggcggtcgcc attggcctcc atgcgaggca gaaccgaagc
     1441 agaaccaagg agagatcgtt tatggcagta cacagggtgc agggctccgt cggtggccgg
     1501 gacatgttcc tggagaccgg caagctcgcc ggccaggccg gcggcgccgt cgtcatcggg
     1561 ctgggcgaga ccgagctgct ggtcaccgcg acagcgggca agcagcccaa ggagcagctc
     1621 gacttcttcc ccctcaccgt cgacgtcgag gagcgcatgt acgccgccgg caagatcccc
     1681 ggcggcttct tccgtcgtga ggggcggccg tcggagacgg ccatcctgac cgcgcggctg
     1741 gtcgaccggc cgctgcgccc ctcgttcccc aaggggctgc gcaacgaggt ccacgtcgtc
     1801 gcgaccatct tgtcggccga catggagaac ccgcccgaca tcctcgcgat caacggggcg
     1861 tctgccgccg tgctgctcgc gggcatcccg ttctcgggac cggtcggggc cgtgcgcctc
     1921 ggccgcacgg gcaccgactg gatcgtcaac cccacctact ccgagctcgc cgagcgcacc
     1981 ttcgacatgg tcgtggccgg ccgccagaac gaggccggcg gagtcgacat cctcatggtc
     2041 gaggccgagg ccaccgagca cgccgtcagg ctgatggagg aggacggcgc gcccaagccg
     2101 accgaggagg ccctcggcct cgcgctcgag ctctccaagt cgcacatcgc gaccgcctgc
     2161 cagctgcagc tcgagctggt ccggcaggcc ggcgcacagc cccgcgagtt cccgctgttc
     2221 gccgagtacg gcgaggacgt cgccggcgcc gtgcggggcg cggtcgaggc ggccacccgc
     2281 gaggcggtgg cgatccccga caagaccgag cggcagaacc gcctcgacga gctgaaggcc
     2341 gaggcccagc aggccgtgtc cggccagctc ggcgacgggc tgaccgggcg cgagaaggag
     2401 atcgcggcgg ccgtccgcag cctcaccaag gagctggtcc gccagcgggt cgtgaaggag
     2461 ggcaggcgca tcgacgggcg cggcccgcgc gacatccggc cgctccaggc cgaggtcggg
     2521 atcctgccga gggcgcacgg ctccggcctg ttccagcgcg gcgagaccca ggtgctgaac
     2581 gtcaccaccc tcggcatggt ccgcatggag cagaccgtcg acaccctcga ccccgaggac
     2641 cgcaagcgct acatgcacca ctacaacttc cccccgttct cgaccggcga ggtcggcttc
     2701 atgcgcggcc cgaagcggcg ggagatcggc cacggggcgc tcgccgagcg cgccctcctg
     2761 ccggtgatcc ccgacaagga cgagttcccc tatgccctgc ggctcgtgag cgaggtgctg
     2821 tcgtccaacg gctccacgtc gatggcgtcg gtgtgcgcct cgacgctgtc gctgatggac
     2881 gccggggtgc cgatcaaggc gcccgtcggc gggatcgcca tgggcctcat cgccgagggc
     2941 ggcgagtacg tgacgctgac cgacatcctc ggggccgagg acgccttcgg cgacatggac
     3001 ttcaaggtgg cgggcacccg cgagttcgtg accgcgctgc agctcgacac gaagctgacc
     3061 ggcatcccga gcgaggtgct gatcgccgcg ctcacccagg cccgcgacgc ccgcctggtg
     3121 gtcctcgacg tcatccaggc cgcgatcgag cggccgcgcg gggagctcaa ccggctcgcg
     3181 ccgcgcgtcc acgtcgagca gatcccggtc gccaagatcg gcgagctgat cggccccaag
     3241 ggcaagcgca tcaacgagat ccaggccgcc tcgggcgccg acatctcgat cgaggacgac
     3301 ggccgcgtgt tcatcggggc gaccgacggc gagcagctcg acatcgccct caggatgatc
     3361 cgcgagcaga tgaacccggt catgccgcag gcgggcgagc gctacctcgg cacggtcgtg
     3421 aagacgacca ccttcggggc gttcatctcc ctcacccccg gcaaggacgg cctgctgcac
     3481 atctccaagc tgggccgcaa gggccagcgg gtgcgtgcgg tcgaggacgt cgtcaacgtg
     3541 ggcgacaagc tgctggtgga ggtggtcgag gtcgaccgcc agaaccgcat ctcgctcagg
     3601 ctcgccgagg agggcgaggc ggcaccgcgg gatggggacg acgccgcccc ggtcgccgcc
     3661 ggcgacggca cccccgacga cgagagccag tagggcccga tgccacgttc gggcgccttc
     3721 gagcggaccg agctcgccgg aggcctgcgg atcctcaccg agcgcatgcc gagcgtgcgc
     3781 tcggtgacgc tcgggatctg ggccggcgtg gggtcgcgcg acgagacccc acgcctggcc
     3841 ggtgcctccc actacctcga gcacctgctg ttcaagggca cctcgcggcg ctccgcccag
     3901 gagatcgccg aggtcatgga cgcggtgggc ggcgagatga acgccttcac gttcaaggag
     3961 tacacctgct tctacgcccg cacgctcgac cgcgacctgc cgctcgcggt ggacgtgctg
     4021 gtcgacatgg tgcgcgactc gaagctcgcc aaggccgacg tcgaggcgga gcggacggtc
     4081 atcctcgagg agatcggcgc ggccaacgac gtgcccgagg acctggtgca cgacctgttc
     4141 gccgaggtgc tgttcggcgg ccacccgctc ggccgccccg tgctcggcac ggccgcgtcg
     4201 atcggcgcca tgcctcgcga cgccatcgcg cgctactggc ggcgccacta cacgccgggc
     4261 aacctcgtgg tcgccgcggc cgggagctgc cggcacgacg aggtggtcga gctgctcgcc
     4321 gaggcgttcg gggacctcgg ggccggcgct ccggcgggcc tccgagggga gcggaaggag
     4381 cccaggttcg ccggtggcgc ctacgtgcgg cgcaagccga ccgaccaggc ccacgtcgtg
     4441 ctcggctgcc cgggcgtgtc gcggtcggac ccgcgccggt tcgcgctcgg ggtgctgaac
     4501 atcgccttcg gcggcggcat gagctcccgg ctgttccagg aggtccgcga gaagcgcggc
     4561 ctggtctact cgatcggctc ctacaccggc cagtactccg agaccggctc gttcgcggtc
     4621 tacgccggag cggcccccag gcggatccac gaggtgctgc gcgtcgtccg cgaggagacc
     4681 gagcgggtcg tggccgacgg gctgaccgac gaggagctgg agcggtccaa ggggcacttc
     4741 aagggctcgc tcgtgctcgg gctcgaggac acgtcgagcc ggatgtcctg gctcggcaag
     4801 tcggagctga cccgcggcga gatcctgtcg gtggacgagg tcatccggcg ggtcgacgcg
     4861 gtcaccatgg cggacgtccg cgcggtcgcc aaggaggtcc tgggcggcgg ccctcgggcc
     4921 ctcgcgctga tcggcccgtt cgaccccgac gggttcgagg agtacgtgtc ttgagaggtg
     4981 cgtggagtcc ttcggacagg caatcgcagc agggtaggtc cagaaaggcg cggagtcctt
     5041 cggacgatcg atccgagcag accggcgacg ccggagagag cgccggcgtc gccggtctgg
     5101 cggtctgcgt gttctgcgcg tccgcgcgcg ggctgcccga gggctaccac cgggccgcgc
     5161 gccggctcgg gcgcgagctg gcgcggcgcg ggcaccggct cgtctacggc gggggcgacg
     5221 ccgggctcat gggtgaggtc gcccgcgcgg tccacgagca cggcggcagg gtcttcggcg
     5281 cgatcccgcg ctcgctggtc gagcgcgagc tcgcctacgg cccggccgac gagctggtgg
     5341 tcaccgagac cctgcgccag cgcaaggcgg agatggacgc ccatgccgac gccttcgtgg
     5401 ccctgccggg cggctacggg acgctggagg agctgctcga ggtgctgacc ctgcgccagc
     5461 tccgcctcca cgaccggccg atcgtgctgc tcaacaccga cggctactac gaccccttcc
     5521 tcgccatggt ccgtggcatg gtcgaccagg gcttcgtccc ggccggcgac ggcaggctct
     5581 tccaggtcgc cgcggccccc gaggaggcac tcgacctggt cgaggccggc gtcggccgtc
     5641 gagcgccgcc cggcgggccg ccggccgagg cgctggccga ggactggacc gcggagctgc
     5701 cagcctcata ggcctggtga gcgagcgggg gggcggtcag ctgaggggga acggcggctg
     5761 gcgccacgcg ggcgtcgcgt cgacctcgat gctggtgacc cacttgaccc accagaagcc
     5821 gcgccggccg ggcaccacca ggcgggccgg gaagccgtgg ccggcggaga ggggcctgcc
     5881 ggcgaccctg gtcgcgagca gcagcgagcc ggcggccgcg aagggcaggc ggcgctggta
     5941 gccggtgcgc gacgtgacca ggatgctctg gccctcgccc atgccggcga gcagccggtc
     6001 gagccgcacc ccctcccagc gcgcggtcgc gtaccacccg ccggtgcagt cgagggcggc
     6061 ctccaccgcg tcggtgcggc ggccgggctc gccgtcggcg agcatgccga gctcgccgta
     6121 ggtgagcgtc cggcgccggc tgccggcccg gacctcgagc cgccagccgg ccgggtcgat
     6181 cgcgggcacc cggtcgtcga gccactgggt gaccgggatg tggtccgggt cggcgctctc
     6241 gacgcgcagg gagccggtga agcgccggcc cgcccctggc agccccgcga cccgtgcgac
     6301 gccctcgagg cccgcccagg ccagcccggc cccgcaggcg agcgcgccgg cccggagcgc
     6361 gttccggcgc gagaggtcgg tgcgccgggg ccggacccgg cgggcggtcg cgtgccaggc
     6421 gagcagcggc acggccgcca gggccgcgcc gacgtggagc tgcatggccg tgacgggccc
     6481 gacggcgccg acccccatgg cgtggagcgt gccggacgcg accgcggcgg cgacgagcac
     6541 ggcgagcggg accgaggcgc cccggcccgg acgccgcctg gcaagcccgc ggcgggcgac
     6601 ggccgacttc cacgggacga ggagcacgag cgcgaggccg gccgctccgt gcgccacgac
     6661 cacgaggcgc ccggcgggcg tgccgagggc gaaggcgagc ccaccggtgg cgagcgccac
     6721 caccagcgac ccgagcagcg cgaggttcgt ccgccggccg gccacggtca acgctccgtc
     6781 gtccctcggc tcgccgcctt cagcctgacg gcggtgcgct cggccgcggt gtcgtcctcg
     6841 ccgaactcgg cgccgaggcg gcggtcgggc acgacggcgt ggtagcggcc gaactcgtgg
     6901 aggccggccg cctcggccac cttggccgag gcgtagttgt cctccgcgtg gttgtagagg
     6961 accgcccgcc cctcggcgag gaccgcacgg gcggccgcct cgaccaccgc gcgcgccagc
     7021 ccccgg
//
LOCUS       DASPYY010000537         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2057931, whole genome shotgun sequence.
ACCESSION   DASPYY010000537 DASPYY010000000
VERSION     DASPYY010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057931"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VEP73_08545"
     CDS             <1..685
                     /locus_tag="VEP73_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26510.1"
                     /translation="GRTALPELAANLEHVLLRPGITPAEVDDACARAVELGLPAVSVW
                     PNAVAQAVAATEGSRVAVVAAVAAPYGTSVRDTKLDEARRAITAGARHLAAAVDAGRF
                     RGGDAGVLAGELAALCYLAHAEGVHVRAVLQAGLLDDQELTIAGRLATEAGADLLQTG
                     FGTAAEPPATVAAVQAARRAVPPRRLGIGVVAGGALDAEAARELLAGGAQRVAVAEPA
                     ALLGRAAAA"
     gene            682..1422
                     /gene="recO"
                     /locus_tag="VEP73_08550"
     CDS             682..1422
                     /gene="recO"
                     /locus_tag="VEP73_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYT26511.1"
                     /translation="MSASGLYREQGVVLRTYRLGEADRIVVLLTQGEGKVRAVAKGVR
                     KTKSRFGGRLEPFSHVDLSLYRGRGELDTVTQVEVIGPFRSLREDYDLVVAGTAMLEA
                     VDQVAQEREAAVRIYLLLVRALRALDGGPRDPSVVLDAFLLKLMALEGYRPSLAECAG
                     CGGPGPYLRFSIARGGALCERCRSGTESTLDERTVPLLAELLGGDMERAAAAAPPRTP
                     RREAGTVVKSFVEYHLDRRLRSYPMVAR"
     gene            1422..2261
                     /gene="uppS"
                     /locus_tag="VEP73_08555"
     CDS             1422..2261
                     /gene="uppS"
                     /locus_tag="VEP73_08555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYT26512.1"
                     /translation="MPSRDPRALPPGLDPRAVPDHVAIVMDGNGRWAKARGLPRNKGH
                     EAGEAALFDTVEGGLDAGLSWLTVYAFSTENWRRPPSEVRFLMNFNESLLVRRSHELH
                     ERGVRIRFIGRRDRVPRHVRRRIEESEELTAGDTRMTLAVALDYGGRDELARAVERLV
                     ADLREGRGPRRIDERAIAERLYTSDMPDVDLLVRTSNERRISNFLLWQIAYAELYFTP
                     VLWPDFDREELFRALRDYQGRQRRFGAVETPPDAPPGTPSTHVRSRENQEQHAWPAAS
                     SST"
     gene            2234..>2482
                     /locus_tag="VEP73_08560"
     CDS             2234..>2482
                     /locus_tag="VEP73_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HYT26513.1"
                     /translation="MASRLLQHVNVVYPTGAEAAARAFYAGTLGLDEVEKPPEFSREG
                     MWFQLEGAGQVHLSPGEPAPRGAWHFCVVADDFDALRGR"
BASE COUNT          294 a          953 c          931 g          304 t
ORIGIN      
        1 tggaaggacc gcattgccgg agctggccgc caacctggag cacgtcctgc tgcgcccggg
       61 gatcaccccg gcggaggtgg acgacgcctg cgcccgtgcc gtcgagctcg gcctcccggc
      121 ggtcagcgtc tggccgaacg cggtcgccca ggcggtggcg gcgaccgagg gcagccgggt
      181 ggcggtggtc gccgcggtcg ccgcccccta cggcaccagc gtgcgcgaca ccaagctcga
      241 cgaggccagg cgcgcgatca ccgccggcgc caggcacctc gccgcggccg tcgacgccgg
      301 ccggttccgg ggcggcgacg ccggcgtgct cgccggcgag ctggccgcgc tctgctacct
      361 cgcccacgcc gagggcgtgc acgtgcgggc cgtgctccag gccgggctgc tcgacgacca
      421 ggagctcacc atcgccggcc ggctcgccac cgaggcaggc gccgacctgc tccagaccgg
      481 gttcggcacc gccgccgagc cgcccgccac cgtcgccgcg gtgcaggccg cccgcagggc
      541 ggtgccgccg aggcgcctcg ggatcggggt ggtcgcgggc ggggcgctgg acgccgaggc
      601 agcgcgcgag ctgctcgcgg gcggggcaca gcgggtggcg gtcgccgagc cggcggccct
      661 cctcggccgg gcggccgccg cgtgagcgcc tcgggcctct accgcgagca gggggtggtg
      721 ctgcgcacct accggctggg cgaggccgac cgcatcgtcg tgctgctcac ccagggcgag
      781 ggcaaggtcc gtgccgtggc caagggggta cgcaagacca agtcccggtt cggcggccgc
      841 ctcgagccgt tctcccacgt cgacctgtcg ctctaccgcg gccgcggcga gctcgacacc
      901 gtgacccagg tcgaggtgat cggcccgttc cgctcgctgc gggaggacta cgacctggtg
      961 gtcgccggga ccgccatgct ggaggccgtc gaccaggtcg cccaggagcg ggaggcggcc
     1021 gtgcggatct acctgctgct cgtgcgcgcc ctgcgcgccc tcgacggcgg gccgcgcgac
     1081 ccctcggtgg tgctcgacgc gttcctgctc aagctgatgg cgctggaggg ctaccggccg
     1141 tcgctggcgg agtgcgccgg ctgcgggggg ccagggccgt acctgcgctt ctcgatcgcg
     1201 cgcggcggcg cgctgtgcga gcggtgccgg tcgggcaccg agtcgacgct cgacgagcgg
     1261 accgtcccgc tgctcgccga gctgctcggc ggggacatgg agcgggccgc cgcggccgcg
     1321 ccgcccagga cgccccggcg cgaggccggc accgtggtga agagcttcgt cgagtaccac
     1381 ctcgaccgcc ggctgcgctc ctacccgatg gtggcgcgct gatgccgagc cgcgatcccc
     1441 gcgcgctccc gcccggcctc gacccgcggg ccgtgcccga ccacgtcgcg atcgtcatgg
     1501 acggcaacgg ccgctgggcc aaggcccgcg gcctgccccg caacaagggc cacgaggcgg
     1561 gggaggccgc cctgttcgac accgtcgagg gcggcctcga cgccgggctg tcctggctca
     1621 ccgtctacgc cttctcgacc gagaactggc ggcgcccgcc ctccgaggtc cgcttcctca
     1681 tgaacttcaa cgagtccctg ctcgtccgcc gctcccatga gctccacgag cggggggtgc
     1741 ggatccggtt catcggccgc cgcgaccgcg tgccgcggca cgtgcgcagg cgcatcgagg
     1801 agtccgagga gctgaccgcg ggcgacacgc gcatgacgct cgcggtcgcg ctcgactacg
     1861 gcgggcgcga cgagctcgcg cgggccgtgg agcgcctcgt cgccgatctg cgggagggcc
     1921 gcggcccgcg ccggatcgac gagcgcgcca tcgccgagcg cctctacacg agcgacatgc
     1981 cagacgtcga cctgctcgtc cgcacgtcga acgagcggcg gatctcgaac ttcctgctgt
     2041 ggcagatcgc ctacgccgag ctgtacttca ccccggtcct gtggcccgac ttcgaccgcg
     2101 aggagctgtt ccgggcgctc cgggactacc agggccgcca gcgccgcttc ggtgccgtcg
     2161 agaccccgcc cgacgccccg ccaggaacgc cgtccaccca tgtccggtcc cgcgagaacc
     2221 aggagcagca cgcatggcca gccgcctcct ccagcacgtg aacgtcgtct acccgaccgg
     2281 ggccgaggcc gcggcgcgcg cgttctacgc cggcaccctc gggctcgacg aggtcgagaa
     2341 gccgcccgag ttcagccgcg agggcatgtg gttccagctc gagggggccg gccaggtgca
     2401 cctgtcgccg ggggagccgg ccccgcgcgg ggcgtggcac ttctgcgtgg tcgccgacga
     2461 cttcgacgcg ctgcgcggcc gc
//
LOCUS       DASPYY010000538         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2198269, whole genome shotgun sequence.
ACCESSION   DASPYY010000538 DASPYY010000000
VERSION     DASPYY010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198269"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..955
                     /locus_tag="VEP73_08565"
     CDS             239..955
                     /locus_tag="VEP73_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26514.1"
                     /translation="MPTPVLSLRGLRRTYGDVVALDGLDLDVAPGECVALIGHNGSGK
                     TTAMRLAAGQLEPTAGRVTVAGVDLHQATDAHRARAQLSFVPDNPVLYADLTVGEHLE
                     LVALAHGVGGEAGDRAAALLDALSLTGRRDFLPPQLSRGMRQKAQLACALVRPFEVLL
                     LDEPVVGLDPPSQATLHELLRSLKADGAGVLLTTHQLGFTAGLADRAVVLSDGRVVDA
                     GPYRRVVAGSAARELGLRSR"
     gene            952..>1698
                     /locus_tag="VEP73_08570"
     CDS             952..>1698
                     /locus_tag="VEP73_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26515.1"
                     /translation="MTLAGGAPMDGVPVRPVAVLLRRRRRSHRRPQQRSDRFIAVYTA
                     ALYATIGGAIAFQAVRQHPAGVAAAGLAAALARWGAAALLLGLMAALRYATWQGPVLF
                     TAPDVAWLLGAPLPRAGLVRPRLRRAFAVGAAAGALVGLGGFTVLEAELGVPGWPLLG
                     AAVAGPALLGLLAVALGWLVESSAARARAVLRASPAAVLAAAAIVLAPGGAGLGRAAL
                     WSGPWGWAVGPVVAAAGGSAPGWPVQLVLLA"
BASE COUNT          142 a          674 c          653 g          229 t
ORIGIN      
        1 ccggccgtgg cgtgcggcac gaacacgtgc agcagcccgt ccccgccggc gccggccgcg
       61 aacgcggcca cccggtcggt caggtcgagg acgtgcctgc cccggttctc gacctccagc
      121 tcgatgctct ccatcgctgc ctccgtgcgt gatgttgcgc agcgggccga ggtccaccaa
      181 ggggccgcgg cgcccagggt cgggagccta acgcgcgcgg acgtactagg gttgggtcgt
      241 gcccactccc gtcctctcgc tgcgcgggct gcgcaggacc tacggcgacg tcgtcgccct
      301 cgacgggctc gacctggacg tcgcgccggg cgagtgcgtg gccctgatcg gccacaacgg
      361 ctccggcaag acgaccgcga tgcgcctcgc ggccgggcag ctcgagccga cggccggcag
      421 ggtgaccgtc gccggggtcg acctccacca ggcgaccgac gcgcaccggg cgcgcgccca
      481 gctgtcgttc gtgcccgaca acccggtgct gtacgccgac ctcacggtcg gcgagcacct
      541 cgagctcgtc gcgctcgccc acggggtcgg cggggaggcc ggcgaccggg cggcggccct
      601 gctcgacgcg ctctcgctga ccgggcgacg cgacttcctg ccgccgcagc tctcccgcgg
      661 catgcgccag aaggcccagc tcgcctgcgc gctcgtccgg ccgttcgagg tgctgctgct
      721 cgacgagccg gtggtcgggc tcgacccgcc gtcacaggcg accctccacg agctgctgcg
      781 gtcgctcaag gcggacggcg ccggcgtgct gctcaccacc caccagctcg gcttcacggc
      841 cggtctggcg gaccgggcgg tggtgctgtc cgacggccgg gtggtcgacg cgggccccta
      901 ccggcgcgtc gtcgccggca gcgcggcccg ggagctcggg ctcaggtcaa ggtgaccctg
      961 gccggcggcg ccccgatgga cggcgtcccc gtccgcccgg tggcggtcct cctccggcgg
     1021 cgccgccggt cccaccgccg cccgcagcag cgctccgacc ggttcatcgc cgtctacacc
     1081 gccgcgctgt acgccaccat cggcggcgcg atcgcgttcc aggcggtgcg ccagcacccg
     1141 gcaggggtgg ccgcggccgg tctggccgcg gcgctcgctc gctggggggc ggcggccctc
     1201 ctcctcgggc tcatggccgc cctgcgctac gccacctggc aggggccggt gttgttcacc
     1261 gcgccggacg tcgcctggct gctcggggcg ccgctgccgc gggcggggct cgtccggccg
     1321 aggctgcggc gcgccttcgc ggtcggcgcc gccgcgggcg ccctcgtagg gctcggcggg
     1381 ttcaccgtgc tcgaggccga gctcggcgtg cccggctggc cgctgctcgg tgccgccgtg
     1441 gccggtccgg cgctgctcgg cctgttggcc gtcgccctcg gatggctcgt cgagtcctcg
     1501 gcggcacgcg cgcgtgccgt cctgcgcgcg agccctgccg cggtgctggc ggcggcggcg
     1561 atcgtcctcg ccccgggcgg cgcgggcctc ggccgcgccg ccctctggtc gggcccgtgg
     1621 ggctgggcgg tcgggccggt cgtggccgcg gcgggcgggt cggcgcccgg ctggccggtc
     1681 cagctcgtcc tgctcgcg
//
LOCUS       DASPYY010000539         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1776929, whole genome shotgun sequence.
ACCESSION   DASPYY010000539 DASPYY010000000
VERSION     DASPYY010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776929"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VEP73_08575"
     CDS             <1..773
                     /locus_tag="VEP73_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYT26516.1"
                     /translation="ALREHVLTGRGHRFAVPPFQGGRADLHLSLLEAIPGTPRVAPLV
                     RARVAGERAGSPNGLTLERALDACGRVAATLHASGIGLGDPRTLDGELAAVRRGLGAV
                     ERLCPPLGELLGEHLQRAEAGAAEAEALPACFAHGDYTPAQVMFQGPVTGLIDFDTVC
                     QAEPALDLGQFLAYLRVLVSKAERSRGQVPGGLAAELGEAFLRVYAQAADVSDAGVLR
                     RRVGAYETVSLVRMALRSWQQLKVDRLENVLGVLEGRR"
     gene            770..2242
                     /locus_tag="VEP73_08580"
     CDS             770..2242
                     /locus_tag="VEP73_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYT26517.1"
                     /translation="MSTSTRLALGDELDPLRSPVLEAELPALATALDGDAMRAHLEAV
                     LAPGGGFERCAAGQATYLGDSCVVRYRVELGGDRSPPALVTGRLFTDRERCARYVEER
                     LAPLAASVGGREELGRFAAPVGTVEPLCMAVAVFPVDGELPTLVDVTDPRRVLGVLRA
                     MVPEAVEGRLTVDGCRVELGHYGRQHRCVLRYHVDATAAGARRRLLVYGKVAGDARGA
                     LAGPVTGALRQRMSGRDARSRFAVPRCLGFVPDLHLALFEAIPGVPQVAQLLKARIQG
                     GPPALDGGPSLGPSLEESVEACARIAAALHTSGIALGPPRTLEDEAGGLRRGLSLVQR
                     VSPGLGERLDAWLRQATDRAAGPGPLPLGFAHGDFSYTQLIFDGARSGLVDFDTVCQA
                     EPALDLGQFLAYLGVAARKARAPGGPGPRLAGQLGARFLAAYAEAAGAPVELAERAAV
                     YEVTSLLRLALHSWQKLKRSRLEHVVDVLDERVPQLAARR"
     gene            2251..3654
                     /locus_tag="VEP73_08585"
     CDS             2251..3654
                     /locus_tag="VEP73_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26518.1"
                     /translation="MDDSLERFATLPPWLVAATDGDRVAAALASAGAERDGAPRVRSC
                     EVDRVRLKGSSWTGVYRLTVDAAAGGPPEVVRVRGTLVPPGAPEPAGATGGTAPGADG
                     WRAYLPGLRLLLEADQGDAALPALPRLTDPGQARALLEEGIRSTPAYAGLRIRGCTPR
                     VMRYKPGSRCTILYELEYPDGAAEPGWPRVVVAKTYHGDKGRVAWDGMRGLWSSPMAS
                     SGVAIAEPLAFLPELNVLVQGPVEEERTLKDELGAALAAGDDAMRGLRGHLAATATGL
                     AALHACGVRLGELVTWEDELAEVREVVARLARPVPELAGAAEPMLARLEAAAAAHPAD
                     PAGPAHRSFRPAQVLLSGERLGFIDFDGFCQAEPALDVALFRATVKGIAMAAGEAGVA
                     PPEGLLARGTELADLFLDRYRERAPLSRTRVALWETLDLFTAVLHGWTKAKPGRLRDA
                     MVVLEDHLAALPAGVRP"
     gene            complement(3644..>4927)
                     /locus_tag="VEP73_08590"
     CDS             complement(3644..>4927)
                     /locus_tag="VEP73_08590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HYT26519.1"
                     /translation="FKPGRKLTAYYDVELGGGARRPVAVTWTPLEGRGALPPEPELDG
                     SAMQAEAEHEGLAAPFRRLRSEVPAWGMRLLVSPLDPRYPQLVRVSAPGHVRDMLSAA
                     DGAGGAAGTGARASRYAVTIVRYRPGQRHVLRYDPADAGRRADGGRAVFAKLYRDGDG
                     ARSHRVAVRVADWLSQARAGVTAARPLAYLPDDEVVLYPQVAGAPLSSRLGRPGDASA
                     GRLGQAGACLRVLQQAPATLAEELPAHDFDAEVRSLARASEHVRPLLPEVGARIADLL
                     DRARELHARLPQEAPAFAHGDYKADHLWVSPRGLTLIDFNTCSLADPALDVGKFLADL
                     HWWHAATDGRGIRRAQQRFLEGYGPEAPPAGARMLRARLYEALVLAKITVRRVRIFDP
                     DWAARTEALVDRADHLLRAAEELRRRRGRGAGVRA"
BASE COUNT          529 a         1913 c         1906 g          579 t
ORIGIN      
        1 ccgccctccg cgagcacgtc ctgaccggac gcgggcaccg gttcgccgtc ccgccgttcc
       61 agggcgggcg tgccgacctg cacctgtcgc tgctcgaggc catccccggc acccctcggg
      121 tcgcgccgct ggtccgggcc agggtcgccg gcgagcgggc agggtccccg aacgggctga
      181 ccctcgagcg tgccctggac gcctgcggcc gggtcgccgc caccctgcac gcctcgggca
      241 tcgggctcgg cgacccgcgc acgctcgacg gtgagctcgc cgccgtccgc cgcggcctcg
      301 gcgcggtgga gcggctctgc cctcccctcg gcgagctgct cggggagcac ctgcagcgcg
      361 cggaggcagg cgcggccgag gccgaggccc tgcccgcctg cttcgcccac ggcgactaca
      421 cccccgccca ggtgatgttc caggggccgg tgaccgggct gatcgacttc gacaccgtgt
      481 gccaggccga gccggccctc gacctgggcc agttcctcgc ctacctccgg gtgctcgtga
      541 gcaaggcgga gcggtcgcgc ggccaggtcc ccggcgggct cgccgcggag ctcggggagg
      601 cgttcctgcg ggtctacgcg caggccgccg acgtctccga cgccggggtc ctgcgacggc
      661 gggtcggcgc gtacgagacc gtcagcctcg tgcgcatggc cctgcggagc tggcagcagc
      721 tcaaggtgga ccggctggag aacgtcctcg gcgtcctcga ggggcgtcga tgagcacctc
      781 cacccgcctg gccctcggcg acgagctgga cccgctgcgc agcccggtgc tcgaggccga
      841 gctgcccgcc ctcgcgacgg cgctcgacgg cgacgcgatg cgggcccacc tggaggccgt
      901 cctcgccccc ggcggcgggt tcgagcgctg cgccgcggga caggccacct acctcggcga
      961 ctcgtgcgtc gtccgctacc gggtggagct cggcggcgac cggtccccac cagccctggt
     1021 gaccgggcgc ctgttcaccg accgggagcg ctgcgcccgc tacgtcgagg agcgcctggc
     1081 ccccctcgcc gcgtcggtgg gcgggcgcga ggagctcggc cggttcgccg cgccggtcgg
     1141 gacggtcgag ccgctgtgca tggcggtcgc ggtgttcccg gtcgacggcg agctgccgac
     1201 gctcgtcgac gtcaccgacc cccggcgggt gctcggcgtc ctccgcgcga tggtccccga
     1261 ggccgtcgag ggccgcctga ccgtcgacgg ctgccgcgtc gagctgggcc actacgggcg
     1321 gcagcaccgc tgcgtgctgc gctaccacgt cgacgccacc gcggccggcg cccggcgccg
     1381 gctgctggtc tacggcaaag tggccggcga cgcccgaggg gcgctcgcgg ggccggtcac
     1441 cggcgcgttg cggcagcgca tgagcggccg tgacgcgcgg tcgcgcttcg ccgtcccacg
     1501 ctgcctcggg ttcgtgcccg acctccatct ggccctgttc gaggccatcc ccggcgtgcc
     1561 ccaggtggcg cagctcctca aggcccgcat ccagggcggg ccgccggcgc tggacggcgg
     1621 cccgagcctc ggcccgagtc tcgaggagtc cgtcgaggcc tgcgcccgca tcgccgccgc
     1681 cctccacacc tctggcatcg cgctcgggcc gccgcgcacc ctcgaggacg aggccggcgg
     1741 gctgcgccgg gggctctcgc tcgtgcagcg cgtctcgccc gggctcggcg agcggctgga
     1801 cgcctggctg cggcaggcca ccgaccgcgc cgccgggccc ggcccgctgc cgctcggctt
     1861 cgcccacggg gacttcagct acacccagct catcttcgac ggggcacgga gcgggctggt
     1921 cgacttcgac accgtctgcc aggccgagcc ggccctcgac ctgggccagt tcctcgccta
     1981 cctgggcgtc gccgcccgca aggcgcgtgc ccccggcggc ccgggccccc ggctggccgg
     2041 gcagctcggc gcccgcttcc tcgctgccta tgccgaggcg gccggcgcgc cggtggagct
     2101 cgcggagcgc gcggccgtct acgaggtcac gagcctgctg cggctggcgc tccacagctg
     2161 gcagaagctc aagcggagcc ggctcgagca cgtcgtcgac gtgctcgacg agcgcgtgcc
     2221 ccagctggcc gcgagaagat aggaggtccc gtggacgaca gcctcgaacg gttcgccacg
     2281 ctcccgccct ggctcgtcgc ggccaccgac ggcgaccggg tcgccgccgc gctggcctcg
     2341 gcgggggccg aacgggacgg ggcaccgcgg gtgcggagct gcgaggtcga ccgcgtgcgc
     2401 ctcaaggggt cgtcgtggac cggcgtctac cgcctcaccg tcgacgcggc ggcgggcggc
     2461 ccgccggagg tcgtgcgggt gcgggggacg ctcgtgccac cgggcgcgcc cgagcccgcc
     2521 ggcgcgaccg gcggcacggc cccgggcgcc gacggctggc gcgcctacct gcctggcctc
     2581 cgcctgctgc tggaggcgga ccagggtgac gcggccctgc cggcgctgcc gcggctgacc
     2641 gatcccggcc aggcccgcgc cctgctcgag gagggcatcc ggtccacccc cgcctacgcg
     2701 ggcctgcgca tcagggggtg caccccgagg gtgatgcggt acaagccggg cagccgctgc
     2761 acgatcctct acgagctcga gtaccccgac ggcgcggccg agccgggctg gccgcgcgtg
     2821 gtggtggcca agacctacca cggcgacaag ggcagggtcg cctgggacgg catgcggggg
     2881 ctgtggagct ccccgatggc ctccagcggg gtcgccatcg ccgagccgct cgccttcctg
     2941 cccgagctga acgtcctcgt ccagggcccg gtcgaggagg agcgcacgct caaggacgag
     3001 ctgggggccg cgctcgccgc cggcgacgac gccatgcgcg ggctgcgcgg ccacctcgcc
     3061 gccaccgcga ccgggctcgc cgcgctgcac gcctgcgggg tgcggctcgg ggagctggtc
     3121 acctgggagg acgagctggc ggaggtccgc gaggtggtcg cccggctggc caggccggtg
     3181 cccgagctcg ccggcgcggc cgagccgatg ctcgcccgcc tggaggccgc cgcggccgcg
     3241 cacccggccg acccggccgg gccggcgcac cggagcttcc gccctgccca ggtcctcctg
     3301 agcggggagc ggctcggctt catcgacttc gacggcttct gccaggccga gcccgccctg
     3361 gacgtggcgc tgttccgcgc cacggtcaag ggcatcgcga tggccgccgg cgaggccggc
     3421 gtggcgccgc ccgagggcct cctcgcccgc ggaacggagc tcgccgacct gttcctcgac
     3481 cgctaccggg agcgggcgcc gctctcgcgg acgcgggtcg ccctgtggga gacgctggac
     3541 ctctttacgg cggtcctgca cggctggacg aaggccaagc ccggccgcct gcgggacgcc
     3601 atggtcgtgc tcgaggacca cctggccgcc ctcccggccg gcgtcaggcc ctgacgccgg
     3661 cgccgcgccc gcgccgccgg cggagctcct cggcggcgcg gaggaggtgg tcggcccggt
     3721 cgacgagcgc ctcggtccgg gcggcccagt ccgggtcgaa gatgcgcacc cgccggacgg
     3781 tgatcttggc gagcacgagc gcctcgtaca ggcgggcgcg gagcatgcgg gcgcctgcag
     3841 gaggcgcctc gggaccgtag ccctcgagga agcgctgctg tgccctccgg atcccccggc
     3901 cgtcggtggc ggcgtgccac cagtggaggt cggccaggaa cttcccgacg tcgagggcgg
     3961 gatcggccag ggagcaggtg ttgaagtcga tcagcgtgag cccgcggggc gagacccaga
     4021 ggtggtcggc cttgtagtcg ccgtgcgcga aggccggggc ctcctggggc agccgtgcgt
     4081 gcagctcccg agcccggtcg aggaggtcgg cgatcctcgc gcccacctcg gggagcaggg
     4141 gccggacgtg ctcgctggcc cgggcgaggg agcgcacctc ggcgtcgaag tcgtgggccg
     4201 ggagctcctc ggcgagcgtg gccggagcct gctgcagcac gcgcaggcag gcgccggcct
     4261 ggcccagccg tcccgccgac gcgtccccgg gacgcccgag gcggctcgag agcggggccc
     4321 ccgcgacctg cgggtacagc acgacctcgt cgtcgggcag gtaggcgagg ggacgggcgg
     4381 cggtgacccc ggcccgcgcc tgcgacagcc agtcggcgac ccggaccgcc acccggtgcg
     4441 agcgggcgcc gtcgccgtcc cggtagagct tggcgaagac cgcccggccg ccgtccgccc
     4501 gccgcccggc gtcggcgggg tcgtaccgca ggacgtgccg ctggcccggc cggtagcgga
     4561 cgatcgtgac ggcgtacctc gacgcccggg cgcccgtccc ggcggctccg cccgcgccgt
     4621 cggcggccga gagcatgtcg cgcacgtgcc cgggcgccga caccctgacg agctgcgggt
     4681 accgggggtc gagcggcgag accaggagcc gcatccccca ggcgggcacc tccgaccgga
     4741 gccgccggaa gggcgcggcc aggccctcgt gctccgcctc ggcctgcatg gccgatccgt
     4801 cgagctccgg ctcgggcggc agggcgccgc gaccctcgag cggcgtccag gtgaccgcga
     4861 cgggccggcg agcccccccg ccgagctcga cgtcgtagta ggcggtgagc ttgcggccgg
     4921 gcttgaa
//
LOCUS       DASPYY010000540         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2058612, whole genome shotgun sequence.
ACCESSION   DASPYY010000540 DASPYY010000000
VERSION     DASPYY010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058612"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEP73_08595"
     CDS             <1..555
                     /locus_tag="VEP73_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYT26520.1"
                     /translation="LAAVAATRAAGAPFEAARAALGRYTGAARRFHLRGEAGGVTIVD
                     DYAHHPTEVAATLAAARLGRWTRVVAVFQPHLYSRTRAFAPELGRALAAADVVLVTDV
                     YAAREDPQPGVDGALVVAAALEARPSLDCAYVPDRNQLAKHVASVVQAGDLLLTLGAG
                     DITTLADEVVELLPRGRGEGGGPG"
     gene            552..1619
                     /gene="murB"
                     /locus_tag="VEP73_08600"
     CDS             552..1619
                     /gene="murB"
                     /locus_tag="VEP73_08600"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYT26521.1"
                     /translation="MSHPGRHDGAPAPGTAAPGPVDAAALDEAWARLAAACPGRVRRD
                     VALAPLTTFRLGGPARLYLQAESVADLAALAGTLAAVPLPLLIVGRGSNMLVADAGWP
                     GVALHLGTRFRGVRVDGVELEAGAATPLPSVASRSAAAGLGGLAFAVAIPGSVGGGVR
                     MNAGAHGSELADRLVWAEVVRLDGPLAGEPTRRKPDELGLGYRRSALPAAAVVTAARF
                     ALHPAPEAEVRAEIDGARRWRRGNQPVNEPNCGSVFANPPEMAAGRAVELLGLKGTRH
                     GGARVSEVHANFIVAAEGATSADVLALIRLAQQRARDEFGITLRTEVQLVGEFGTPAG
                     ERPAARRPDGPGAEPRAEVEG"
     gene            1616..2419
                     /locus_tag="VEP73_08605"
     CDS             1616..2419
                     /locus_tag="VEP73_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYT26522.1"
                     /translation="MTSRPAADRRIVERRRQVAADRVRRRRRQLALGVAAVLLAAGVA
                     RLASSPLFSLTSVRVAGTAALTPAEVLAASRVRPGMPYLALDPGVIRRRVETLPRVAR
                     AEVHRVYPSSLRIAVVERPATAAIAAGGRWWQVAADGTVLEAASSRPAGLPFVAHVPV
                     PAGLRPGTKLPANGPLANALSALGGLSPQLARQVTGVEARSVDSLAFRLRDRSTVLYG
                     LAEAQPAKDAAALLLLGKLARQGRKVVLVDVRNPSAPTVSQGKKPATGG"
     gene            2627..3772
                     /gene="ftsZ"
                     /locus_tag="VEP73_08610"
     CDS             2627..3772
                     /gene="ftsZ"
                     /locus_tag="VEP73_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYT26523.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEASLKGVEFIAVNTDA
                     QALLASDADVKLDVGRELTRGLGAGSNPDVGRQAADDHAEELEEVLKGADMVFVTAGE
                     GGGTGTGGAPVVARLSKAQGALTIGVVTRPFAFEGRRRSAQADQGIERLKAEVDTLII
                     IPNDRLLQVAERTTSMLDAFKLADQVLLQGVQGITDLITIPGLINLDFADVRATMREA
                     GTALMGIGQARGEDRAVAAAKQAISSPLLEASVDGARGVLLNISGGSDLGLFEVNEAA
                     EVIASAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDRTEEEPEAAPPVARPARPGGG
                     ETVWPSPASRRAEERRQPEPVGPGREPPRRPALDDDDDLDIPSFLKR"
     gene            3792..4727
                     /gene="pgeF"
                     /locus_tag="VEP73_08615"
     CDS             3792..4727
                     /gene="pgeF"
                     /locus_tag="VEP73_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HYT26524.1"
                     /translation="MRPARPPGPAGPAVASARQGTGLEWRPGPPGAGGATPAVAVAWP
                     PVLVAEDLLEHGVVAAFSSRAGGLGRPPYDQLNLGLHVGDDVRTVLANRRRVATVLGL
                     AGLPWATVRQVHGASVAVAARDRFGQGPPEAKPALAEADALVTAEGGVVLAVLAADCV
                     PVLLADPAGRVVAAAHAGWKGLAAGVVEAAVAAFARAGGDPSAAVALVGPAVGPCCYE
                     VGPEVLAAVGDRYPGAVAATRDGRPSLDLAAAAAEGLRRSGLERVRGAGECTRDHPGR
                     YFSHRRDGTTGRQAGLVALVPGRSGRPGARGGVPR"
     gene            4724..>5142
                     /locus_tag="VEP73_08620"
     CDS             4724..>5142
                     /locus_tag="VEP73_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYT26525.1"
                     /translation="MTAPGLARPHPDPGEAAVADGVRRVRERIAAAAGRAGRDPAEVT
                     LVAVAKLHPVEAIRRALDAGVTDVGESYAQELEAKAAALAGAPVRWHFVGRLQRNKAG
                     TVVAAGALVHSLASVATARALGRRAHAQGTVARALVQV"
BASE COUNT          557 a         2038 c         1971 g          576 t
ORIGIN      
        1 ctcgcggccg tcgcggccac gcgcgccgcc ggggcgccct tcgaggccgc gagggccgcc
       61 ctcggccggt acaccggcgc ggcccggcgc ttccacctcc gcggcgaggc aggcggggtc
      121 acgatcgtcg acgactacgc ccaccacccg accgaggtcg ccgccaccct cgccgcggcc
      181 cggctggggc gctggacgcg ggtggtggcg gtgttccagc cccacctgta ctcgcgcacc
      241 cgggcgttcg cccccgagct cggccgcgcc ctcgccgcgg ccgacgtggt gctggtcacc
      301 gacgtctacg ccgcgcgcga ggacccgcag ccgggcgtgg acggcgccct cgtcgtagcg
      361 gccgcgctcg aggcgcgccc gagcctcgac tgcgcctacg tgcccgaccg caaccagctc
      421 gccaagcacg tcgccagcgt cgtgcaggcg ggcgacctgc tgctcaccct cggcgcgggc
      481 gacatcacca ccctggccga cgaggtggtc gagctgctcc cgcggggccg cggcgaaggc
      541 ggggggccgg gatgagccac cccgggcgcc acgacggtgc gccagcaccc ggcaccgccg
      601 cgcccgggcc ggtggacgcc gccgccctgg acgaggcctg ggcgcgcctg gcggcggcct
      661 gcccggggcg ggtgcggcgc gacgtcgcgc tcgcccccct cacgaccttc cgcctcgggg
      721 gaccggcgcg gctctacctg caggccgagt cggtcgccga cctggccgcc ctggccggga
      781 cgctcgcggc ggtgccgctg cccctcctga tcgtcggcag gggctccaac atgctcgtgg
      841 ccgacgcggg ctggccgggc gtcgcgctcc acctcgggac gcggttccgg ggggtccggg
      901 tcgacggggt cgagctcgag gcgggcgcgg ccacgcccct gcccagcgtg gcgtcacgct
      961 ccgcggcggc cggcctcggc ggcctcgcct tcgccgtcgc catcccgggc agcgtcggcg
     1021 gcggcgtccg gatgaacgcc ggcgcgcacg ggtcggagct ggccgaccgc ctggtgtggg
     1081 ccgaggtggt ccggctggac ggccccttgg cgggggagcc cacgcggcgc aagccggacg
     1141 agctgggcct cggctaccgc cgctcggccc tgcccgccgc cgcggtggtc accgcggcgc
     1201 gcttcgcgct ccacccggcg cccgaggcgg aggtgcgggc cgagatcgac ggggcgcgcc
     1261 gctggcggcg cggcaaccag cccgtgaacg agccgaactg cggcagcgtg ttcgccaacc
     1321 cgcccgagat ggccgccggc cgcgcggtgg agctgctcgg cctgaagggg acgcgccacg
     1381 gcggcgcgcg ggtcagcgag gtccacgcca acttcatcgt ggcggccgag ggcgccacct
     1441 cggccgacgt gctcgcgctc atccggctcg cccagcaacg agcccgcgac gagttcggca
     1501 tcaccctgcg caccgaggtc cagctggtcg gcgagttcgg cacccccgcg ggcgagcggc
     1561 cggcggcgcg ccggccggac gggcccggcg ctgagcctcg agcggaggtc gaaggttgac
     1621 ctcgcggccg gccgccgacc gccgcatcgt cgagcggcga cgccaggtcg cagccgaccg
     1681 cgtccggcgc cgccgcaggc agctcgccct cggcgtcgcc gccgtcctgc tcgcggccgg
     1741 ggtggccagg ctcgcgagct cgccgctgtt ctcgctcacc tcggtgcggg tcgcgggaac
     1801 cgccgcgctc acccccgccg aggtgctcgc cgcctcgcgc gtccgtcccg gcatgcccta
     1861 cctggccctc gacccgggcg tcatccgccg gcgggtggag acgctgcccc gcgtcgcgcg
     1921 ggcggaggtg caccgggtgt acccgtcgag cctgcgcatc gccgtcgtgg agcgcccggc
     1981 cacggcggcc atcgccgccg ggggccgctg gtggcaggtt gccgccgacg ggacggtcct
     2041 cgaggccgcc tcgtcgcggc ccgccggcct gcccttcgtc gcgcacgtgc cggtgcccgc
     2101 cggcctgcgc ccggggacga agctcccggc gaacggcccg ctggccaacg ccctcagcgc
     2161 gctcgggggg ctcagccccc agctcgcgcg ccaggtcacc ggcgtcgagg cgcgctcggt
     2221 ggacagcctc gcgttccgcc tgcgggaccg ctcgacggtc ctgtacgggc tggccgaggc
     2281 acagccggcg aaggacgcgg cagccttgct gctgctcggc aagctcgcac gccaaggaag
     2341 gaaggtggtg ctcgttgacg tgcgcaaccc gtccgcgcca acggtctccc agggcaagaa
     2401 accagccacc ggtggttgac cggccccagg gccgccggta ccatcggccc cggcccgagg
     2461 ttgacataac cgtggacctc aggtcgaggc ttcggcagct tccccccttt ccctggctct
     2521 cggcgctggg cgacccagtg gccgtggctg accggcgcgg tcccgacgag tacggccgtg
     2581 aagggacgag aacgtccgga accgggcgga ggaggacgat cgaatgatgg cggcgccgca
     2641 gaactacctg gccgtgatca aggtggtcgg gatcgggggc ggcggcgtca acgccgtcaa
     2701 ccggatgatc gaggccagcc tcaagggcgt cgagttcatc gccgtcaaca ccgacgcgca
     2761 ggccctgctc gcctccgacg ccgacgtcaa gctcgacgtc ggccgggagc tcacccgcgg
     2821 gctcggcgcc ggctccaacc ccgacgtcgg ccgccaggcc gccgacgacc acgccgagga
     2881 gctcgaggag gtgctgaagg gcgccgacat ggtcttcgtc accgcgggcg agggcggcgg
     2941 cacgggcacc ggcggcgccc cggtggtcgc gcgcctgtcc aaggcccagg gggcgctgac
     3001 gatcggggtg gtcaccaggc ccttcgcgtt cgaggggcgg cgccgctcgg cccaggccga
     3061 ccagggcatc gagcggctca aggccgaggt cgacaccctc atcatcatcc cgaacgaccg
     3121 cctgctccag gtcgccgagc ggaccacctc gatgctcgac gccttcaagc tcgccgacca
     3181 ggtgctcctg cagggcgtgc aggggatcac cgacctcatc accatccccg gcctcatcaa
     3241 cctcgacttc gccgacgtgc gggcgacgat gcgggaggcg gggacggcgc tcatgggcat
     3301 cggccaggcg cgcggcgagg accgcgcggt ggccgccgcg aagcaggcga tctcctcccc
     3361 gctgctcgag gcgtcggtcg acggcgccag gggcgtgctg ctcaacatct cgggcggctc
     3421 cgacctcggg ctgttcgagg tgaacgaggc cgccgaggtg atcgcctcgg cggcccaccc
     3481 ggacgccaac atcatcttcg gcgccgtcat cgacgacgcg ctcggcgacg aggtgcgcgt
     3541 caccgtgatc gcggccgggt tcgaccgcac cgaggaggag ccggaggccg ccccgcccgt
     3601 cgccaggccg gcaaggccgg gcgggggcga gacggtgtgg ccctccccgg cgtcgcgccg
     3661 cgccgaggag cgacgccagc ccgagccggt cggacctggg cgggagccgc cgcgcaggcc
     3721 ggcccttgac gacgatgacg acctcgacat cccctccttc ctcaagcgct gaccgtggcg
     3781 ctccggtccc ggtgcgtccc gcgcgaccgc cggggcccgc gggcccggcg gtcgcgtcgg
     3841 cgcgccaggg gaccggcctc gagtggcggc ccggcccgcc cggtgccggc ggcgcgacac
     3901 cggccgtcgc cgtggcctgg ccgcccgtgc tggtcgccga ggacctcctc gagcacggcg
     3961 tggtcgcggc cttctcctcc cgcgccggcg ggctcgggcg gccgccctac gaccagctga
     4021 acctcggcct gcacgtcggc gacgacgtcc gcaccgtgct ggccaaccgc cgccgggtgg
     4081 cgaccgtcct cggcctggcc ggcctgccgt gggcgaccgt gcgccaggtc cacggtgcct
     4141 ccgtggcggt cgccgcccgc gaccgcttcg gccaggggcc gcccgaggcc aagccggcac
     4201 tggccgaggc cgacgccctc gtgaccgccg agggcggtgt ggtgctcgcc gtgctcgccg
     4261 ccgactgcgt gcccgtgctg ctcgccgacc ccgccggccg ggtcgtggcg gccgcccacg
     4321 ccggctggaa gggcctggcc gccggcgtgg tcgaggcggc cgtggccgcg ttcgcccgcg
     4381 ccggcggcga cccgagcgcc gccgtggcgc tcgtggggcc ggccgtcggc ccctgctgct
     4441 acgaggtcgg gccggaggtg ctggccgccg tcggcgaccg ctacccgggc gccgtggccg
     4501 ccaccaggga cggccggccg tccctcgacc tcgccgccgc ggccgccgag gggctgcggc
     4561 gctcggggct cgagcgcgtg aggggcgccg gagagtgcac ccgcgaccat cccggccgct
     4621 acttctccca ccggcgggac ggcaccacgg gtcgccaggc cggcctcgtc gccctcgtcc
     4681 ccgggcggag cgggaggccg ggcgcccgcg gcggggtgcc gcgatgactg cccccggcct
     4741 cgcgcggccg catcccgatc ccggggaggc cgcggtcgcc gacggcgtca ggcgcgtgcg
     4801 cgagcgcatc gccgccgcgg ccgggcgggc cggccgcgac cccgccgagg tcacgctcgt
     4861 cgccgtggcc aagctgcacc cggtggaggc gatccggcgc gccctcgacg ccggggtgac
     4921 cgacgtcggc gagagctacg cccaggagct cgaggccaag gccgcggcgc tcgccggggc
     4981 accggtgcgc tggcacttcg tcgggcgcct gcagcgcaac aaggcgggga ccgtggtcgc
     5041 cgccggggcg ctcgtccact cgctcgcctc ggtggcgacc gcccgcgcgc tcgggcgccg
     5101 ggcgcacgcc caagggacgg tcgcgcgggc cctcgtgcag gt
//
LOCUS       DASPYY010000541         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_934452, whole genome shotgun sequence.
ACCESSION   DASPYY010000541 DASPYY010000000
VERSION     DASPYY010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934452"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VEP73_08625"
     CDS             <1..1602
                     /locus_tag="VEP73_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYT26526.1"
                     /translation="GHWLEMRALGQASSALDALAALLPDEAELVTDGGTRTVPTTELD
                     VGDVVLVRPGSRVPADGVVVDGRADVDESMITGESRPVAKGPGGRVVAGTVVTDSAIR
                     VRVEAVGEQTALAGIRRLVEQAQASRSRAQALADRAAALLSYFATAAGLVTFAAWLAL
                     GRPDQAVERTVTVLVIACPHALGLAIPLVIAISTAMAARNGILVKDRLALERMRTVDA
                     VLFDKTGTLTKGEPRVGGVAAVDGDEDGLLRLAGAVETDSEHPLARASDFHSRTGRGV
                     TARVGTATVAVGGPALLRELGAAEPEALAEQVAAWKRRGAAVLYVVCDDKVLGALALE
                     DAVRPESREAVDGLHRLGKRVVMVTGDARQVAEAVAAELGVDEVFAEVLPEDKDAAVA
                     DLQRRGLKVAMVGDGVNDAPALARADVGIAIGAGTDVAIESAGIALASNDPRGVLSVI
                     RLSQASYRKMLQNLGWATGYNLLGIPLAAGALAWAGITLAPAVGATLMSASTIVVALN
                     AQLLRRIDLRPTAGLRPGGRRPSGR"
BASE COUNT          202 a          664 c          638 g          208 t
ORIGIN      
        1 ggccactggc tggagatgcg cgccctcggg caggcctcca gcgcgctcga cgccctcgcg
       61 gcgctgctgc cagacgaggc cgagctcgtc acggacggcg gcacccgaac cgtccccacc
      121 accgagctgg acgtcggcga cgtggtgctg gtccgcccgg gcagccgggt gcccgccgac
      181 ggcgtcgtgg tcgacggccg ggccgacgtc gacgagtcga tgatcaccgg cgagtcccgt
      241 cccgtggcca agggccccgg cggccgggtg gtggccggca cggtcgtcac cgactccgcc
      301 atccgggtcc gggtcgaggc ggtcggggag cagaccgcgc tcgccggcat ccgccggctg
      361 gtcgagcagg cccaggcctc gcgctcccgc gcgcaggccc tggccgaccg ggccgccgcc
      421 ctgctgtcct acttcgccac cgccgccggg ctggtcacct tcgccgcctg gctggctctc
      481 ggccgtcccg accaggcggt cgagcggacc gtgaccgtgc tggtgatcgc ctgtccccac
      541 gcgctgggcc tggccatccc gctggtcatc gcgatctcca ccgccatggc ggcacgcaac
      601 ggcatcctgg tcaaggaccg cctggccctg gagcggatgc ggaccgtcga cgcggtgctg
      661 ttcgacaaga ccggcaccct gaccaagggc gagccccgtg tgggcggcgt ggccgcggtc
      721 gacggcgacg aggacgggct gctgcggctg gccggggcgg tcgagaccga ctccgagcac
      781 ccgctggccc gcgcgagcga cttccactcg aggaccggcc gcggcgtcac cgcccgagtg
      841 gggacagcca cggtggcggt gggcgggccg gcgctgctgc gcgagcttgg cgccgccgag
      901 cccgaggcgc tcgccgagca ggtcgccgcc tggaaacggc gtggcgccgc cgtcctctac
      961 gtcgtctgcg acgacaaggt cctcggggcg ctggccctgg aggacgcggt ccggcccgag
     1021 tcccgcgagg cggtcgacgg gctgcaccgg ctcggcaagc gggtggtgat ggtcaccggc
     1081 gacgcccgcc aggtcgccga ggcggtcgcc gccgagctcg gcgtcgacga ggtgttcgcc
     1141 gaggtcctgc ccgaggacaa ggacgccgcc gtggccgacc tgcaacgccg cggcctcaag
     1201 gtggcgatgg tcggcgacgg ggtcaacgac gccccggcgc tcgcccgcgc cgacgtcggc
     1261 atcgcgatcg gcgccggaac cgacgtcgcc atcgagtcgg ccgggatcgc gctggcctcc
     1321 aacgacccgc gcggggtcct cagcgtcatc cgcctgtccc aggcgagcta ccgcaagatg
     1381 ctgcagaacc tcggctgggc gaccggctac aacctgctcg gcatcccgct ggccgccgga
     1441 gcgctggcct gggccggcat cacccttgcc cccgccgtcg gggcgacgct catgagcgcc
     1501 tccaccatcg tggtcgccct caacgcccaa ctgctgcgcc gcatcgacct acggcctaca
     1561 gccggccttc gaccgggagg tcggcgaccc tccggccgct gacccggacg gtgcgccgga
     1621 gcaggtcgcg gactccgacg agctggccgg cggcctgtcc gagcccgtcg ggagggtgcc
     1681 gcccggctcc ccctccggcg gggccgggcc gc
//
LOCUS       DASPYY010000542         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1356674, whole genome shotgun sequence.
ACCESSION   DASPYY010000542 DASPYY010000000
VERSION     DASPYY010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356674"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VEP73_08630"
     CDS             complement(<1..951)
                     /locus_tag="VEP73_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HYT26527.1"
                     /translation="MILEGRTILVTGGTGSLGQVLVRRVLSGELGTPRKVVVLSRDEA
                     KQHAMRLSYLHKSVTTDEVIYRNFSRALEFRIGDVRSYHDVCSALRDADFVVNAAALK
                     QVPTCEYFPDQAVMTNCQGAINLVRAIREHDYPVQAVVAVSTDKACKPVNVMGMTKAL
                     QERIVVAANIGRTATRFMVVRYGNVLASRGSVIPLFHSQIEHGGPVTVTTPEMTRFLV
                     TLDEAVDMVFRALREGHPGETLVPITPSATVMDIAMALVGDRNVEIRVTGIRPGEKLH
                     EVLVSEEECNHTTRQGRYYVIAPMLPELAPDGAGEAADLKG"
     gene            complement(948..1820)
                     /locus_tag="VEP73_08635"
     CDS             complement(948..1820)
                     /locus_tag="VEP73_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT26528.1"
                     /translation="MRLLVVGGDGMLGHRLLVQLAPVHDVRVTLRRPLAAYAPYRLYS
                     ERNAFPGVDVRDEAAVRGVLARFRPEAVVNAAGIVKQRPEATQSIPSIQVNALFPHRL
                     ALACRETGARLVHLSTDCVFSGRRGGATEASEPDAQDLYGRTKLLGEVDDGACVVLRS
                     SIVGLELANRRGLVEWFLARRGGTVRGWRHAVWSGLSTAEMTRVIDRVLGRHPELTGL
                     WHVASEPVSKYDLLRRLAGHLGRDVRVEPDDSLRCDRSLDAGRFRQATGWEPPTLDEM
                     LGELAGEIREREAT"
     gene            complement(1817..>2957)
                     /locus_tag="VEP73_08640"
     CDS             complement(1817..>2957)
                     /locus_tag="VEP73_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT26529.1"
                     /translation="SFPSRAPFAGYGPFRPWSERLGTVAVTRVPLASRGRGQGWRLAL
                     NYLSFASSASLLGPARLRGRFDVIFVYQLSPATVGLPAAVLKRLKGTPVVVWVQDLWP
                     ESLAAAGAVTSERVLGWVGRLVRWLYRQSDCVLVTSRGFLDHVDALGARPPRVAYLPQ
                     WAERGYAPVDVAEDAPERRELPAGFRVVLAGNIGTAQSLGTVLEAAELLRATCDVHWV
                     VIGDGRRRAWVEAEVARRGLAGRVHLLGRRPVEAMPRYLALADALVVTLERREIYALT
                     IPARLQSFLACGKPVLGALDGEGARVIHESGAGIAVPAEDAPALAGAVRAVHGLPAAE
                     RAAMGARGRAYFLEHFERQKLLDELEACLLETASGRGDHRDGGDR"
BASE COUNT          396 a         1090 c         1104 g          367 t
ORIGIN      
        1 gcccttcagg tcggcggcct ccccggcccc gtcgggggcc agctccggca gcatcggcgc
       61 gatcacgtag taccgcccct gcctggtggt gtggttgcac tcctcctcgg agacgagcac
      121 ctcgtgcagc ttctcgcccg ggcggatgcc ggtcaccctg atctccacgt ttcggtcgcc
      181 gacgagcgcc atggcgatgt ccatgacggt cgccgacggg gtgatgggga cgagcgtctc
      241 ccccgggtgc ccctcgcgga gcgcccggaa gaccatgtcc acggcctcgt ccagggtgac
      301 gaggaagcgg gtcatctccg gggtggtcac ggtcaccggc ccgccgtgct cgatctggct
      361 gtggaacagc gggatcaccg acccgcggga ggcgagcacg ttgccgtagc ggacgaccat
      421 gaaccgcgtc gcggtccgcc cgatgttggc cgcgacgacg atccgctcct gcagcgcctt
      481 ggtcatgccc atgacgttca cgggcttgca cgccttgtcg gtggagaccg cgacgacggc
      541 ctggaccggg tagtcgtgct cccggatggc gcgcacgagg ttgatcgcgc cctggcagtt
      601 ggtcatcacc gcctggtcgg ggaagtactc gcaggtcggc acctgcttga gcgccgcggc
      661 gttgaccacg aagtcggcgt cccgcagcgc cgagcagacg tcgtggtagc tgcgcacgtc
      721 gccgatccgg aactcgaggg cgcgggagaa gttgcggtag atcacctcgt cggtggtcac
      781 cgacttgtgc aggtaggaca ggcgcatggc gtgctgcttg gcctcgtcgc gggacagcac
      841 cacgaccttg cgtggcgtgc ccagctcgcc ggagaggacg cgcctgacca ggacctggcc
      901 gagcgacccg gtcccgccgg tgacgaggat cgtcctgccc tcgaggatca cgtggcctcc
      961 cgttcgcgga tctccccggc gagctcgccg agcatctcgt ccaaggtcgg cggctcccag
     1021 ccggtcgcct gccggaagcg cccggcgtcg aggctccggt cgcaccgcag cgagtcgtcc
     1081 ggctccaccc gcacgtcgcg gccgaggtgg ccggcgagcc ggcggagcag gtcgtacttg
     1141 ctgaccggct cggacgcgac gtgccacagg ccggtcagct ccgggtgcct gccgagcacc
     1201 cggtcgatca cgcgggtcat ctccgcggtc gagaggccgc tccagacggc gtggcgccag
     1261 ccgcggacgg tcccgccgcg ccgggccagg aaccactcga ccaggccgcg acggttggcg
     1321 agctcgagcc ccacgatcga ggagcgcagc acgacgcacg cgccgtcgtc gacctcgccg
     1381 aggagcttgg tcctgccgta gaggtcctgg gcgtccggct cgctggcctc ggtcgcgccg
     1441 ccccgccgcc ccgagaagac gcagtcggtg gacaggtgga cgagccgcgc gccggtctcg
     1501 cggcaggcga gcgcgagccg gtgcgggaac agggcgttca cctggatgct cgggatgctc
     1561 tgggtcgcct cgggccgctg cttgacgatc ccggcggcgt tcacgaccgc ctcgggccgg
     1621 aaccgggcca ggacgccgcg caccgccgcc tcgtcgcgca cgtcgacgcc cgggaaggcg
     1681 ttccgctcgg agtacaggcg gtacggcgcg taggcggcga gcggccgccg cagggtgacc
     1741 cgcacgtcgt gcaccggcgc gagctgcacc agcagccggt gcccgagcat gccgtccccc
     1801 ccgaccacga gcagcctcat cggtcccccc cgtctcggtg atcccctctg ccgctggcgg
     1861 tctcgagcag gcacgcctcc agctcgtcga ggagcttctg gcgctcgaag tgctcgagga
     1921 agtaggcgcg gcccctcgcg cccatcgccg cccgctccgc ggcgggcagg ccgtggacgg
     1981 cgcgcaccgc gccggccagg gccggcgcgt cctcggcggg cacggcgatc ccggcgcccg
     2041 actcgtggat cacccgcgct ccctcgccgt ccagcgcgcc gagcaccggc ttgccgcagg
     2101 ccaggaacga ctgcagccgg gcggggatgg tcagggcgta gatctcccgg cgctcgaggg
     2161 tcacgacgag cgcgtcggcc agggccaggt agcggggcat ggcctcgacc gggcggcgcc
     2221 cgaggaggtg cacgcggccg gcgagcccgc gccgggccac ctcggcctcg acccaggccc
     2281 gccgcctgcc gtcgccgatc accacccagt ggacgtcgca cgtggcgcgc agcagctcgg
     2341 cggcctcgag gacggtgccg agggactgcg cggtgccgat gttgccggcc agcacgacgc
     2401 ggaagccggc ggggagctcg cggcgctcgg gcgcgtcctc ggcgacgtcg acgggggcgt
     2461 agccgcgctc cgcccactgg gggaggtagg cgaccctcgg cggccgcgcg ccgagcgcgt
     2521 cgacgtggtc caggaaccct cgcgaggtca cgaggacgca gtcgctctgc cggtagagcc
     2581 agcgcaccag ccggcccacc cagccgagca cccgctcgga ggtgacggcc ccggcggcgg
     2641 cgagcgactc cggccacagg tcctgcaccc agacgacgac gggcgtgccc ttgagccgct
     2701 tgaggacggc cgccggcagc ccgacggtgg ccggggagag ctggtagacg aagatgacgt
     2761 cgaagcgccc gcgcagccgc gccgggccga gcaggctcgc cgacgacgcg aacgacaggt
     2821 agttgagcgc gagccgccac ccctggccgc ggccccgtga ggcgaggggg acgcgcgtga
     2881 ccgcgaccgt gccgagccgc tccgaccacg gccggaaggg gccgtagccg gcgaacgggg
     2941 cgcggctcgg gaagctc
//
LOCUS       DASPYY010000543         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2339223, whole genome shotgun sequence.
ACCESSION   DASPYY010000543 DASPYY010000000
VERSION     DASPYY010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339223"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /gene="glgP"
                     /locus_tag="VEP73_08645"
     CDS             <1..1686
                     /gene="glgP"
                     /locus_tag="VEP73_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYT26530.1"
                     /translation="IRRLIEDERLDAATATEAVRGGTIFTTHTPVSAGIDRFPRSFIE
                     RYFGPGGVATGLSVDQIMDLGAEPDADRSMFNMAVMGLRLAQRSNGVSRLHGEVSRDM
                     FRGLWPGFPPSEVPIGHVTNGVHAGTWVNRDWSELFDRRIGPGWRSADTGWEGLLDAS
                     DEEVWQVRAAARRRLVADVRARLTAAWRARGAIQGQLGWIDRAFDPEVLTVGFARRVP
                     SYKRLTLLLRDPERLTRLLLDRERPIQLLIAGKAHPADEGGKSLIADFARFAADAKVR
                     HRIAFLPDYDMAMARTLVAGADVWLNNPLRPYEACGTSGMKAALNGCLNLSVRDGWWD
                     ELYDGANGWAIPSADDPSIPADRRDELEAAAIYDLLEHEVLPLFYDQRSPAGVPTAWV
                     AKIKHNFTSLGPAVLASRMVRDYTERLYLPAAAGSRQLRADAYGPSRDLADWKRRVAG
                     AWPGVRVRRVEAVDADHTLGAWLPIRASVELGGLDPDEVDVQVAFGRVDDTDELADTD
                     LAPLKHNGHLDGAWNYEGEIPLTIPGAFGYTVRVTPRHQGIANAAELGLVAWA"
     gene            complement(1735..2235)
                     /locus_tag="VEP73_08650"
     CDS             complement(1735..2235)
                     /locus_tag="VEP73_08650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT26531.1"
                     /translation="MARILSLDGVAAAAWATRGAGTPPDAYSGAAVDVSTASLADLDG
                     LVALWTAVGLRFRHDLVAEELAGVLERDPDLVLVVEEGGTIIASVLGTWDGRRGWVTR
                     LATHPDHRGRGLALQLVTELEERLRAKGCRKLNLLIEQHNAAVVPYYERLGYRRDDLI
                     FMEKFL"
     gene            complement(2254..>2627)
                     /locus_tag="VEP73_08655"
     CDS             complement(2254..>2627)
                     /locus_tag="VEP73_08655"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HYT26532.1"
                     /translation="ATAPDPPPAARRPEAPGRHRTPWRLACSLCGEGKAQGRRLVAGP
                     GVALCDRCVPAALEVLVEGTGPDPRLRPVTEDPAGWRACSFCGKELRRVGRLVQGPEG
                     VHVCGECLDLSTEILREEAGS"
BASE COUNT          339 a          998 c          966 g          324 t
ORIGIN      
        1 atccgccggc tgatcgagga cgagcggctg gacgcggcca ccgcgaccga ggcggtccgg
       61 ggcgggacca tcttcacgac ccatacgccg gtgtccgcgg ggatcgaccg gttcccgcgg
      121 tcgttcatcg agcggtactt cgggccgggc ggggtcgcga ccgggttgtc ggtcgaccag
      181 atcatggacc tcggcgccga gcccgacgcc gaccggtcga tgttcaacat ggccgtcatg
      241 ggcctgcggc tggcgcagcg gtccaacggg gtgagccggc tgcacggcga ggtctcccgc
      301 gacatgttca gggggctgtg gccggggttc ccgccgtcgg aggtcccgat cgggcacgtc
      361 accaacggcg tgcacgcggg gacgtgggtg aaccgggact ggtcggagct gttcgaccgc
      421 aggatcgggc cgggatggcg cagcgccgac accggctggg aagggctgct cgacgcgtcc
      481 gacgaggagg tctggcaggt ccgggccgcc gcccgccgcc ggctggtcgc cgacgtccgc
      541 gcccggctga ccgcggcctg gcgggcgcgc ggcgcgatcc aggggcagct cggctggatc
      601 gaccgcgcct tcgaccccga ggtgctgacc gtgggcttcg cccgccgggt gccgtcctac
      661 aagcggctca cgctgctgct gcgcgacccc gagcggctca cgcgcctgct gctcgaccgc
      721 gagcggccga tccagctcct catcgcgggc aaggcccacc cggccgacga gggcgggaag
      781 tcactgatcg ccgacttcgc ccggttcgcg gccgacgcca aggtccgcca ccggatcgcg
      841 ttcctgcccg actacgacat ggccatggcc cgcaccctgg tcgccggcgc cgacgtgtgg
      901 ctcaacaacc cgctccggcc ctacgaggcg tgcgggacca gcgggatgaa ggcggccctg
      961 aacggctgcc tgaacctgtc ggtccgggac ggctggtggg acgagctcta cgacggcgcc
     1021 aacggctggg cgatcccctc cgccgacgac ccgtcgatcc ccgccgaccg ccgcgacgag
     1081 ctggaggcgg cggcgatcta cgacctgctc gagcacgagg tcctgccgct gttctacgac
     1141 cagcgttccc cggccggcgt gcccaccgcc tgggtcgcga agatcaagca caacttcacc
     1201 tccctcggcc cggccgtgct ggcctcccgg atggtccgcg actacaccga acggctctac
     1261 ctgccggccg cggccggctc ccggcagctc cgcgccgacg cctacggccc ctcccgggac
     1321 ctggccgact ggaagcgccg cgtcgccgga gcatggccgg gcgtgcgcgt ccgccgggtg
     1381 gaggcggtcg acgccgacca caccctcggc gcctggctgc ccatccgggc cagcgtcgag
     1441 ctcggcggcc tcgaccccga cgaggtcgac gtccaggtcg cgttcggccg ggtcgacgac
     1501 accgacgagc tcgccgacac cgacctcgcg cccctcaagc acaacggcca cctcgacggc
     1561 gcctggaact acgagggcga gatccccctc accatcccgg gcgccttcgg ctacacggtc
     1621 cgcgtcacgc cccgccacca gggcatcgcc aacgccgccg agctcggcct ggtcgcctgg
     1681 gcgtaggccc cgccgtgccg ggcggggcac cgccaggctg tgcgtttgcc ctggtcagag
     1741 gaacttctcc atgaagatga ggtcgtcgcg gcggtagccc aggcgctcgt agtaggggac
     1801 gacggcggcg ttgtgctgct cgatcaggag gttcagcttg cggcagccct tcgcgcgcag
     1861 ccgctcctcc agctcggtga cgagctggag ggcgaggccg cggccgcggt ggtcggggtg
     1921 cgtcgcgagg cgggtcaccc agccccgcct cccgtcccag gtgccgagga cggaggcgat
     1981 gatcgtgccg ccctcctcga ccacgaggac gaggtcgggg tcacgttcga gcacgcccgc
     2041 cagctcctcg gcgacgaggt cgtgccggaa gcggagcccg acggccgtcc acagggcgac
     2101 caggccgtcg aggtcggcga gcgatgcggt gcttacgtcc actgcggccc cactgtaggc
     2161 gtcaggcggc gtccccgccc cgcgcgtcgc ccacgccgcg gccgccacgc cgtccaggga
     2221 gaggatgcgc gccacctggg ccccgacagc gggtcaggag ccggcctcct cgcgcaggat
     2281 ctcggtgctg aggtcgaggc actcgccaca gacgtggacg ccctccgggc cctgcacgag
     2341 ccggccgacc cggcggagct ccttgccgca gaagctgcac gcccgccacc cggccgggtc
     2401 ctcggtcacg ggccggagcc gagggtccgg gccggtgccc tcgacgagga cctcgagcgc
     2461 ggccggcacg cagcggtcgc agagcgcgac gccggggccg gcgacgagcc ggcgcccctg
     2521 tgccttcccc tcgccacaca gcgagcaggc gagccgccag ggggtccggt gccggccggg
     2581 cgcctccggc cggcgggccg ccggcggcgg gtccggggcg gtcgcag
//
LOCUS       DASPYY010000544         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1217733, whole genome shotgun sequence.
ACCESSION   DASPYY010000544 DASPYY010000000
VERSION     DASPYY010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217733"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEP73_08660"
     CDS             complement(<1..332)
                     /locus_tag="VEP73_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HYT26533.1"
                     /translation="MSQPPADLRAPADHPSLDPRTPVVVGVGQSSERLGEPGYRRRSP
                     VDLAADAAREALDDTGADPAAVAAAIDVVVGIRQFENSHPRARAALGRSDNYPRSVAG
                     RIAATPARA"
     gene            complement(329..1453)
                     /locus_tag="VEP73_08665"
     CDS             complement(329..1453)
                     /locus_tag="VEP73_08665"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYT26534.1"
                     /translation="MRTAFTDLVGVQHPIVGFNRSPGVVAAVTNAGGFGVLGASAYTP
                     AELDAQLTWIDEQTAGKPYGVDLLVPEKFATGDPGNLVASLRAQIPDEHLAFVSDLLD
                     RYGVPEVAGNPEHDEIAASLNPDGAAPLLDVAFRHPIRLIANALGPPPPGLVDRARKQ
                     GVPVAALVGQPRHAVKQIEAGVDVLIAQGTEAGGHTGTIATMVLTPEIVDIAGGRPVL
                     AAGGIASGRQMAAALALGAAGVWCGSVWLSSEEDVANQAIKAKFLAATSSDTERSRTR
                     TGKPARQLRSAWHEEWERPGSPAPLPLPLQPMLVNEAWQRIDAAARAGHEGAVRLESF
                     FVGQVVGSFEQIRPAGEITRQIVADCEQRIRDLAKLEAGS"
     gene            complement(1453..>2207)
                     /locus_tag="VEP73_08670"
     CDS             complement(1453..>2207)
                     /locus_tag="VEP73_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014927125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYT26535.1"
                     /translation="GGLGGVVPPGASTATQAQAPPAPVPVSEPGRRRGKWSEHDARTL
                     LGEAGIPVVPARLAGSAAEAVKAAADFGGPLSVKIVSADILPKSDIGGVVLDVPADEE
                     AVRAAYRAVIAAVPDGARIDGVLVSPMRRGGTELLVGVVRDPQWGPVLAVAIGGLFVE
                     VLRDSALAPLPVTQGQAGRLLDRLRGRAVLDGVRGAAPADRDALTGVIARIGDLALAL
                     GDALESLEVNPLLVDGARIEALDAGLTWREHG"
BASE COUNT          270 a          798 c          857 g          282 t
ORIGIN      
        1 gcgcgggctg gggtcgcggc gatccgtccc gccacggacc gggggtaatt atccgaacgg
       61 cccagcgctg cccgcgcccg cggatgggaa ttctcgaact ggcggatgcc gactaccaca
      121 tcgatcgccg ccgcaaccgc ggcggggtcg gccccggtgt cgtccagtgc ctcgcgggcc
      181 gcgtcggcgg ccaggtcgac cggcgagcgc cgccggtagc cgggctcgcc gaggcgttcg
      241 gaggactgcc cgacgcccac gacgacgggc gtgcgcgggt cgagggaggg gtggtccgcg
      301 ggcgcacgga gatcggcggg cggctggctc atgagcccgc ctcgagcttg gcgagatcgc
      361 ggatccgctg ctcgcagtcg gccacgatct ggcgggtgat ctcgccggcc ggcctgatct
      421 gctcgaagga gcccacgacc tggccgacga agaaggactc gagccggacc gcgccctcgt
      481 ggcccgcccg ggccgccgcg tcgatccgct gccacgcctc gttgaccagc atcggctgca
      541 gcggcagggg cagcggcgcc gggctgccgg ggcgttccca ttcctcgtgc caggcgctgc
      601 gcagctggcg ggcgggcttg cccgtcctgg ttcgcgagcg ctccgtgtcc gagctggtgg
      661 ccgccaggaa cttggccttg atggcctggt tggccacgtc ctcttcgctg gacagccaga
      721 ccgacccgca ccagacgccg gccgcgccga gggccagggc ggcggccatc tggcggccgg
      781 acgcgatgcc gccggcggcc agcaccgggc gcccgcccgc gatgtcgacg atctccgggg
      841 tgagcaccat ggtggcgatg gtgccggtgt ggccgccggc ctcggtgccc tgcgcgatca
      901 gcacgtcgac gccggcctcg atctgcttga ccgcgtgcct gggctgcccg accagggcgg
      961 cgacgggcac gccctgcttc ctggcccggt cgacgaggcc aggcggcggc gggccgaggg
     1021 cgttggcgat aaggcggatg ggatggcgga aggccacgtc gagcaggggg gccgcgccat
     1081 cggggttgag gctggcggcg atctcgtcgt gctcggggtt gcccgcgacc tcggggaccc
     1141 cgtagcggtc gagcagatcg gagacgaagg ccaggtgctc gtccgggatc tgggcgcgca
     1201 ggctcgccac gaggttgccc gggtcgcccg tcgcgaactt ctccgggacg agcaggtcga
     1261 cgccgtaggg cttgcccgcg gtctgctcgt cgatccaggt cagctgggcg tcgagctcgg
     1321 ccggggtgta ggccgacgcg cccagcacgc cgaagccgcc ggcgttggtg accgcggcca
     1381 ccacgccggg cgagcggttg aagccgacga tcgggtgctg gacgccgacc aggtcggtga
     1441 aggcggttct catcagccgt gctccctcca ggtgaggccg gcatcgagcg cctcgatccg
     1501 ggcgccgtcg accagcagcg ggttgacctc gagcgattcg agggcgtcgc ccagggccag
     1561 ggcaaggtcg ccgatccggg cgatcacccc ggtcagggcg tcgcggtcgg cgggcgcggc
     1621 gccgcggacc ccgtccagca ccgcccggcc gcgcagccgg tccagcaggc ggccggcctg
     1681 cccttgtgtc accggcagcg gcgccagcgc cgaatcccgc agcacctcga cgaacagccc
     1741 accgatcgcc acggccagga ccgggcccca ctgcgggtcg cggacaacgc cgacgaggag
     1801 ttcggtgccg ccccggcgca tcgggctgac caggacgccg tcgatcctgg cgccgtccgg
     1861 gacggccgcg atcacggccc ggtaggccgc gcggacggcc tcctcgtcgg ccgggacgtc
     1921 cagtacgacg ccgccgatgt cgctcttggg caggatgtcc gccgagacga tcttgaccga
     1981 cagcgggccg ccgaagtcgg cggcggcctt gaccgcttcg gcggccgaac cggccagccg
     2041 ggccgggacg accgggatgc ccgcctcgcc gagcagcgtg cgggcgtcgt gttcggacca
     2101 cttcccccgg cgccggccag gctcggacac tggtacaggt gcgggcggtg cctgggcttg
     2161 tgtggctgtg ctggctccgg gggggacgac ccccccgagc ccccccg
//
LOCUS       DASPYY010000545         1907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_934882, whole genome shotgun sequence.
ACCESSION   DASPYY010000545 DASPYY010000000
VERSION     DASPYY010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934882"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..661)
                     /locus_tag="VEP73_08675"
     CDS             complement(41..661)
                     /locus_tag="VEP73_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYT26536.1"
                     /translation="MASGREQYLQALASLTEMTRNQAERVAARLARQGELQSGQVSRV
                     AEDLLRRTQKNRETVSRLVQREVKRQLGMLGIATRDEVARLQQRIRALEQELDRAGGA
                     GGRAGPAGRRASTGRPARTATQATAKTTAAKPTAAEGSAAKARATTRTAGASTRSSAA
                     GGRGTTRRSTAAPGDTAAPGDTAAPGGGDAPGGGDAAGGGDAPSQS"
     gene            852..1193
                     /locus_tag="VEP73_08680"
     CDS             852..1193
                     /locus_tag="VEP73_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYT26537.1"
                     /translation="MASKAEVERQLGVLLARLDDNRDGVRSAIPTRKVLRCHVPDLDA
                     SWFSVIEDGHVSPPSERSPDGRSDITLHVGSDDLVDLIEGRLSFLSAFTSGKVKVDAS
                     IMDMLRLRALL"
     gene            1230..>1907
                     /locus_tag="VEP73_08685"
     CDS             1230..>1907
                     /locus_tag="VEP73_08685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYT26538.1"
                     /translation="MISLIPIHDQNPTRRPAVVTYLLIAANVIVFLLEPVTTSVSGDQ
                     GSVVAECRQIAFFRKYAAIPRELTTNKPLGVTAGPPAGPNACRPVRATYHKIPFLSVL
                     TAMFLHGGWLHLLGNMLFLYVFGNNVEDRLGHLRFLLFYIACGYVAAYGFALTNPNSS
                     ATLVGASGAIAGVLGAYLVLYPRARVTSLVPFLLFLPVRLPAWIVLGSWFVLQWLYYQ
                     GAGMGQGS"
BASE COUNT          213 a          734 c          663 g          297 t
ORIGIN      
        1 gctcgtcgga cgggggcagg ccggccacgg ggtgccgccg tcagctctgg ctgggcgcgt
       61 cgcccccgcc ggccgcgtcc ccgcccccgg gggcgtcccc gcccccgggg gcggcggtgt
      121 ccccgggggc ggcggtgtcc ccgggggcgg cggtcgaccg cctggtcgtc ccgcgcccgc
      181 ccgcggccga cgacctggtg ctcgcgccgg cggtccgggt ggtcgccctg gccttggccg
      241 cggagccctc ggccgcggtc ggcttggccg ccgtggtctt ggcggtggcc tgcgtcgcgg
      301 tccgcgccgg cctgcccgtc gaggccctgc ggccggccgg ccccgccctg ccacccgcgc
      361 ccccggcccg gtcgagctcc tgctcgaggg cacggatacg ctgctgcagg cgcgcgacct
      421 cgtcgcgggt ggcgatgccg agcatgccga gctgccgctt cacctcccgc tggaccagcc
      481 ggctcaccgt ctcgcggttc ttctgggtgc gccgcaggag gtcctcggcg acccggctga
      541 cctgcccgga ctggagctcg ccctgcctgg ccagccgcgc ggcgacccgc tcggcctggt
      601 tcctggtcat ctcggtcaag ctcgccaatg cctgcaggta ctgctccctg ccgcttgcca
      661 cctggcacct ccgaactccg gacgcccgag ctccggacgc tgcactcgct gcactccgga
      721 cgctgcgatt ctagcaacgc gcccggccgg cgaggacccg cggcggcttg ctggtggatc
      781 cgtgtggtag aacccgttca ctcccggggg cctcggaagg tccggtaggg gcgagcgcga
      841 ggaggacggc ggtggcgagc aaggccgagg tcgagcggca gctcggggtg ctgctcgccc
      901 gcttggacga caaccgggac ggggtccggt cggcgatccc gacccgcaag gtgctgcggt
      961 gccacgtccc cgacctcgac gcgtcctggt tctcggtgat cgaggacggc cacgtctcgc
     1021 cgcccagcga gcgctctccc gacggccgca gcgacatcac cctgcacgtc ggctccgacg
     1081 acctcgtcga cctcatcgag ggacggctgt cgttcctgtc cgccttcacg tcgggaaagg
     1141 tcaaggtcga tgcgagcatc atggacatgc tgcgcctgcg ggccctgctc taggccgcgg
     1201 gtaggcgaac ggcagggagg gacgcgccga tgatcagcct catcccgatc cacgaccaga
     1261 acccgacccg ccggcccgcg gtcgtcacct acctgctgat cgccgccaac gtcatcgtct
     1321 tcctgctgga gccggtcacc acgtcggtgt cgggcgacca gggcagcgtg gtcgccgagt
     1381 gccgccagat cgccttcttc cgcaagtacg ccgcgatccc gcgcgagctc acgacgaaca
     1441 agccgctcgg cgtcaccgca gggccgccgg ccggaccgaa cgcctgccgg ccggtccggg
     1501 ccacctacca caagatcccg ttcctgtccg tgctcacggc catgttcctg cacggcgggt
     1561 ggctccacct gctcggcaac atgctgttcc tctacgtgtt cggcaacaac gtcgaggacc
     1621 gcctcggcca cctccgcttc ctgctgttct acatcgcctg cggctacgtg gccgcatacg
     1681 ggttcgcgct caccaacccg aacagcagcg cgacgctcgt cggcgcgtcg ggcgcgatcg
     1741 ccggggtgct cggcgcctac ctcgtcctgt acccgagggc gagggtcacc agcctggtgc
     1801 cgttcctgtt gttcctgccg gtccggctgc ccgcctggat cgtgctcggg tcctggttcg
     1861 tgctgcagtg gctgtactac cagggcgccg gcatgggcca gggctcg
//
LOCUS       DASPYY010000546         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_90960, whole genome shotgun sequence.
ACCESSION   DASPYY010000546 DASPYY010000000
VERSION     DASPYY010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90960"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VEP73_08690"
     CDS             complement(<1..198)
                     /locus_tag="VEP73_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT26539.1"
                     /translation="MLYGRREEHARVAALLAGARVGRSGVLVVRGEAGVGKRALLEDA
                     AEQAAGFQLLRGAGVESEMELA"
     gene            complement(486..749)
                     /locus_tag="VEP73_08695"
     CDS             complement(486..749)
                     /locus_tag="VEP73_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HYT26540.1"
                     /translation="MALNRRLRVDPIACDGHGLCAQLLPERISLDDWGYPILDPTPVG
                     PELTAHARRAVNACPVLALALERPKAREASGRRPTFARQAGTG"
     gene            complement(740..2101)
                     /locus_tag="VEP73_08700"
     CDS             complement(740..2101)
                     /locus_tag="VEP73_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYT26541.1"
                     /translation="MSGVAGVRGPETRAGAGPVGAPGSPRLLSAAGEHGLPLARHRLV
                     YPAPPPPGGRARDGLVTTVQRAGLRGRGGASFPTGTKLEAVRAGGRRPVVVVNGSEGE
                     PASGKDKLLLASAPHLVLDGALLAAAAVGADLVTVCVDRTATAALGAVERALGERRQA
                     ADRPGVDAETGVPVRLVAVPPRYVAGEETALVHWLNGGPAAPTSTPPRPFQRGVDGRP
                     TLVDNVETLAHLAQVARWGPDWFRQLGTREEPGTALVTLSGAVDRPGVYEVPVGTPLA
                     GLVRAAGVPGGVGAVLVGGYFGGWLDPGRAGRASLDNQCLRSLGASLGCGAVVVLPAG
                     ACGLSESARVLAWLAGETAGQCGPCVHGLAAVAAAVADVRDGRGGDGGLDRLRRWAGQ
                     IEGRGACRLPDGAVRLLRSTLDVFADDVRRHLHHGGCAGAARPPLLPVPARDPGPGRS
                     PWR"
     gene            complement(2098..2655)
                     /locus_tag="VEP73_08705"
     CDS             complement(2098..2655)
                     /locus_tag="VEP73_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HYT26542.1"
                     /translation="MNGILAATPALWYLTRGSGAVVLVLLTVTVALGVTTSVRWASPR
                     WPRSVTEGLHRNVSLLVVAFLALHVATTVVDGYAPIGWLDAVVPFRSAYRPLWLGLGA
                     VALDLLAAVAVTSLLRARLGRRTWRAVHWLAYACWPVALAHGLGTGSDTGRAWMLALD
                     AVAVAAVATAVGWRLWTGFGQPVSS"
     gene            complement(2652..>3099)
                     /locus_tag="VEP73_08710"
     CDS             complement(2652..>3099)
                     /locus_tag="VEP73_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYT26543.1"
                     /translation="GGVLVSLGGDLATAGTPPPGGWAVRVADWHAAGRGAQGQTVRVR
                     GGGLATSSTTVRRWTRGGEQLHHVVDPATGRPAPVVWRTVSVAAATCVDANVAATAAI
                     VRGDRSPAWLDSLGLPARLVRPDGSLVRVGGWPAPDPDLARAATAP"
BASE COUNT          378 a         1167 c         1262 g          292 t
ORIGIN      
        1 cgccagctcc atctccgact cgaccccggc gccgcgcagg agctggaacc cggcggcctg
       61 ctcggccgcg tcctcgagca gggcgcgctt gccgacgcct gcctcgccgc ggaccacgag
      121 cacgccgctg cgccccaccc gtgcgcccgc gagcagcgca gcgacgcggg catgctcctc
      181 gcgccgtccg tacagcatgt gatccagtcg gggtcaaggg cgacgagtgg tggaggacga
      241 gcggcggaag gccaagcgcc cgagatcgag cgtccgccac ccattgcacc atatgagatc
      301 gagcggtatc agcggctgac ggacgcccca atgtcgccgt gacctgccgt ggcgcaccgc
      361 cgcggagatg gcccggctgg gccgcctcga cccggcccgc cgacctgggc accgccgagg
      421 gagtcgagac ccccgagcgg ttcgagcagc tgcgcgcgct gggctgcgac cacgggcagg
      481 gctgctcagc ccgtgccggc ttggcgggcg aacgtgggcc gccgtccgga ggcctccctg
      541 gccttcggcc gctccagtgc cagggcgagg acagggcagg cgttgaccgc gcggcgggcg
      601 tgggcggtga gctcggggcc gaccggggtc gggtcgagga tcgggtagcc ccagtcgtcc
      661 aggctgatgc gttcggggag cagctgggcg cacaggccgt gcccgtcgca tgcgatcggg
      721 tccacgcgca gccggcggtt cagcgccatg gtgaccttcc tgggccggga tcccgggcgg
      781 ggacggggag cagaggcggg cgggcggcgc ccgcgcagcc gccgtggtgg aggtggcgcc
      841 ggacgtcgtc ggcgaacacg tcgagggtgc tgcgcaggag ccgcacggcg ccgtcgggca
      901 gccggcaggc gccgcggccc tcgatctggc cggcccagcg ccgcaggcgg tcgaggccgc
      961 cgtcgccgcc acggccgtcg cgcacgtcgg cgacggcggc ggcgacggcg gccaggccgt
     1021 ggacgcacgg gccgcactgg ccggccgtct cgccggccag ccaggcgagg acgcgggccg
     1081 actcgctgag cccgcaggcg cccgcgggga gcacgacgac cgcgccgcag ccgaggctcg
     1141 cgcccagcga gcgcaggcac tggttgtcga ggctggcacg gcccgcccgc cccgggtcga
     1201 gccagccgcc gaagtagccg ccgaccagca ccgccccgac gccaccggga acgccggcgg
     1261 cgcgcaccag gccggcgagg ggggtgccga cggggacctc gtagacgcct ggccggtcga
     1321 cggcgccgga gagcgtgacg agcgcggtgc ccggctcctc acgggtgccg agctggcgga
     1381 accagtccgg cccccagcgg gcgacctggg cgaggtgggc cagggtctcg acgttgtcga
     1441 ccagtgtggg gcggccgtcg acaccccgct ggaacgggcg cggcggtgtg ctggtgggcg
     1501 cggccggccc gccgttgagc cagtgcacca gcgcggtctc ctccccggcc acgtaccgcg
     1561 gcgggaccgc gaccagccgc accggtacgc ccgtctccgc gtcgacgcct ggccggtcgg
     1621 cggcctgccg gcgctcgccg agcgcccgct cgacggcgcc gagcgcggcg gtggcggtcc
     1681 ggtcgacgca gacggtcacc aggtcggcgc cgaccgcggc cgcggcgagc agcgccccgt
     1741 cgaggacgag gtgcggggcg ctggcgagca gcagcttgtc cttgccgctg gccggctcgc
     1801 cttcggagcc gttgaccacc acgaccgggc ggcgcccgcc ggcgcgcacg gcctcgagct
     1861 tcgttccggt ggggaagctc gccccgcccc tgccccgcag gccggcgcgc tggacggtgg
     1921 tgaccaggcc gtcgcgggcg cggccgccgg ggggcggtgg cgccgggtag acgagccggt
     1981 gacgggcgag cggcaggcca tgctcgcccg cggcggacag cagccgggga ctccccggcg
     2041 cgcccaccgg gccggcgccg gcgcgcgtct ccggccccct gacgccggcg acgccgctca
     2101 tgacgacacc ggctggccga agccggtcca caggcgccac cccacggcgg tcgcgaccgc
     2161 cgcgaccgcc accgcgtcga gcgcgagcat ccacgcccgg ccggtgtcgc tgcccgtgcc
     2221 gagcccgtgg gccagcgcga ccggccagca ggcgtaggcc agccagtgca cggcccgcca
     2281 ggtccggcgg ccgaggcggg cgcgcagcag gctggtgacg gcgaccgcgg cgagcaggtc
     2341 gagggcgacc gctcccaggc cgagccagag cggccggtac gcggagcgga acggcaccac
     2401 cgcgtccagc cagccgatcg gtgcgtagcc gtcgacgacg gtggtggcga cgtgcagggc
     2461 gaggaaggcg accaccagca gcgagacgtt gcggtgcagc ccttcggtca cggagcgggg
     2521 ccagcgcggc gacgcccagc ggaccgaggt ggtgaccccg agtgcgacgg tgacggtcag
     2581 caggacgagg acgaccgcgc cgctgccgcg ggtcaggtac cacagcgcgg gggtggcggc
     2641 gaggatgccg ttcatggcgc cgtggccgcc cgtgcgagat ccgggtcggg cgcgggccag
     2701 ccgccgaccc ggacgagcga cccgtcgggg cggaccaggc gggccgggag gccgagcgag
     2761 tcgagccatg ccgggctgcg gtcgccgcgg acgatcgccg cggtggccgc gacgttggcg
     2821 tcgacacagg tggccgcggc cacgctcacg gtgcgccaca ccaccggcgc gggccggccg
     2881 gtggcggggt cgacgacgtg gtggagctgc tcgccgccgc gcgtccagcg gcgcacggtc
     2941 gtgctcgacg tggccagccc gccgccgcgc acgcggacgg tctggccctg ggcgcctcgg
     3001 ccggcggcgt gccagtcggc gacccgcacc gcccagccgc cgggaggcgg cgtcccggcg
     3061 gtggcgaggt cgccgccgag gctgaccagc acgccaccg
//
LOCUS       DASPYY010000547         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2480751, whole genome shotgun sequence.
ACCESSION   DASPYY010000547 DASPYY010000000
VERSION     DASPYY010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480751"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1841
                     /locus_tag="VEP73_08715"
     CDS             30..1841
                     /locus_tag="VEP73_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26544.1"
                     /translation="MLRRLRERQEWKFFGVLPKADRTLALAWWALLVLRGALPAVFAV
                     ATGMLVGAVQGGQDLAGALALMAVVFLLLQVLAPLHQATSANLGSRVAAWLYDRLTEA
                     CVRPPGMGHLEDPRLAGDLTVAREFDLGMTGPPMFINIDFIAGSLVEMIGGLASALVL
                     SAYAWWAPIVLAGGWLATHWLLRESSVWRDRNTDEVRQAQRHAEYAYRLAVDPPAAKE
                     LRLFGLAGWAIERFTSRRTLLFELQYQATRLRERPVVWSLLIVTAANVAVFWSLASGA
                     ADGRLDLGRLVVFAQVAVGTSMIAFGGLNWALDGAAAPVAAVLRLEPAMGPAGALVAP
                     TRPPRRADGMPAREVRFRALTFAYPRSERPVLEGFDLAIPAGSSLAIVGQNGAGKTTL
                     AKLLCRLYDPQGGAIEVDGVDLRDLDLEGWRARLAAVFQDFIRFELSLRDNVAPAGAP
                     DETVLAALAEAGAGGLAALDAPLARGYQGGTELSGGQWQRIALARALCAVRLGAGLVL
                     LDEPTAQLDVRGESEIFDRILAATRRATTILISHRFSTVRHADRICVLEQGRVIELGT
                     HDELMAEGGRYRTMFELQAKRFGATEDEEGTAYDLLA"
     gene            1825..>2499
                     /locus_tag="VEP73_08720"
     CDS             1825..>2499
                     /locus_tag="VEP73_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26545.1"
                     /translation="MTSWRDGLPPALPSMWRLLKLGYRHEPWLLVAAFGLSQIAALPD
                     ALLALWLKLLGEGLLQDDRGRVLVAAVGLGLSATATWFLRTASTRVQRRFRDKVTVAL
                     ESHVARLQASLATIAHQERPDYLDRLSVLRNQVFVLDHMYMSLFATCGWILRLGVTVG
                     LLMSIHPALALLAVFALPTVLTSTWRPGVERTAEERVAQAGRLAQHLFMTATTAPPGK
                     EVRVTGI"
BASE COUNT          287 a          944 c          891 g          377 t
ORIGIN      
        1 gcaccgggtt agacgggagg gcttggccgg tgctgcgtcg gctgcgcgag cgacaggagt
       61 ggaagttctt cggcgttctg cccaaggccg accgcaccct cgcccttgcc tggtgggccc
      121 tcctggtgct ccggggcgcg ctccccgcgg tgttcgcggt cgccacggga atgctcgtgg
      181 gcgcggtgca gggcggccag gacctggcgg gggcgctggc tctcatggcc gtggtcttcc
      241 tgctcctgca ggtgctcgcg ccgctgcacc aggcgacgag cgccaacctc gggagccggg
      301 tcgcggcctg gctgtacgac cggctgaccg aggcgtgcgt ccgcccgccg ggcatgggcc
      361 acctcgaaga cccccggctc gccggcgacc tcacggtcgc gcgcgagttc gacctgggca
      421 tgaccgggcc gccgatgttc atcaacatcg acttcatcgc ggggagcctg gtcgagatga
      481 tcggcggcct ggcctcggcg ctcgtgctct ctgcctatgc gtggtgggcg cccatcgtgc
      541 tggccggggg ttggctcgcg acccactggc tcctgcgcga gagctcggtg tggcgcgatc
      601 gcaacaccga cgaggtgcgc caggcgcaac gccacgcgga gtacgcctac cgactcgcgg
      661 tggacccgcc cgccgccaag gagctgcgcc tgttcggcct cgccgggtgg gccatcgagc
      721 gcttcacctc gcgccggacg ctcctgttcg agctccagta ccaggcaacg cgcctgcgcg
      781 agaggccggt cgtctggagc ctgctcatcg tcaccgccgc caacgtcgcc gtcttctggt
      841 cgctggccag cggagccgcg gacggccgcc tcgacctggg ccgcctggtc gtcttcgcgc
      901 aggtggcggt gggcacgagc atgatcgcct tcggcggcct caactgggcg ctcgacggcg
      961 cagccgcccc cgtcgccgcg gtgctgcggc tcgagccggc catgggcccc gcgggcgccc
     1021 tcgtcgctcc gacgaggccc cctcgccggg ccgacggcat gccggcgcgc gaggtccgct
     1081 tccgcgctct caccttcgcc tatccccgga gcgaacggcc ggtgctcgag ggcttcgacc
     1141 tcgcgattcc cgcgggctcg tcactcgcga tcgtcggcca gaacggtgcg gggaagacca
     1201 ccctcgccaa gctgctgtgc cgactctacg atccccaagg cggcgccatc gaggtggacg
     1261 gcgtcgacct gcgcgacctc gacctggaag gctggcgggc gcggctcgcc gccgtgttcc
     1321 aggacttcat ccgcttcgag ctgtcgctgc gcgacaacgt ggctcccgcg ggcgcccccg
     1381 acgagacggt gctcgcggcc ctggcggagg cgggggcggg cgggctggcc gccctcgacg
     1441 cgccgctcgc ccggggctac cagggcggca ccgagctgtc gggcgggcag tggcagcgca
     1501 tcgccctcgc ccgggccctg tgcgcggtgc gcctcggagc ggggctggtc ctgctcgacg
     1561 agcccaccgc ccagctcgac gtgcggggcg aatccgagat cttcgaccgg atcctcgccg
     1621 cgacccgccg ggccaccacg atcctgatct cccaccgctt ctccaccgtg cgccacgccg
     1681 accgcatctg cgtgctcgag cagggccggg tgatcgagct cggcacccac gacgagctga
     1741 tggccgaagg aggccgctac cgcacgatgt tcgagctgca ggccaagcgc ttcggcgcca
     1801 ccgaggacga ggaagggacg gcctatgacc tcctggcgtg acggcctgcc gccggctctc
     1861 ccgtcgatgt ggcgcctgct caagctcggc taccgccacg agccgtggct gctggtggcc
     1921 gcgttcgggt tgtcccagat cgcggccctg cccgacgccc tgctggccct ctggctgaag
     1981 ctgctcgggg agggcctgct ccaggacgac cggggccggg tgctcgtggc cgcggtgggc
     2041 ctcggcctgt ccgccaccgc cacctggttc ctgcgcacgg cgagcacccg cgtgcagcgg
     2101 cgcttccggg acaaggtcac ggtcgccctc gagtcccacg tggcccggct gcaggcgtcg
     2161 ctcgcgacca tcgcccacca ggagcggccc gactacctcg accgcctctc cgtgctgcgc
     2221 aaccaggtgt tcgtgctcga ccacatgtac atgtcgctct tcgccacctg cgggtggatc
     2281 ctgcgcctgg gggtcacggt cgggctgttg atgtcgatcc atcccgccct ggccctgctc
     2341 gccgtgttcg cgctgcccac ggtgctgacc tcgacctggc ggccgggcgt cgagcggacg
     2401 gccgaggagc gggtcgccca ggccggccgg ctggcccagc acctgttcat gaccgccacc
     2461 accgcccccc ccggcaagga ggtgcgcgtg accgggatc
//
LOCUS       DASPYY010000548         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1920347, whole genome shotgun sequence.
ACCESSION   DASPYY010000548 DASPYY010000000
VERSION     DASPYY010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920347"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VEP73_08725"
     CDS             complement(<1..756)
                     /locus_tag="VEP73_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT26546.1"
                     /translation="MSTPTSLELPDGVRRTTIRTARGAFAALEAAPASGVCERDAALL
                     VPGYTGSKEDFLAILDLLADDSRHVVAIDMRGQFETAGADEPGGYAAAALGADILAIM
                     HATGARHLVGHSYGGLIGREAVLAAAGAEFASFTLMSSGPGALTGPRARDLRAMLASL
                     GAGDGGRPGAAAPDKDAPDKDAPDKDALRAGIAELWRSYLEPQAIAAGVPGHIVAFLG
                     RRMRDNDPDGLVLMAAHMLAAPDRTAELARLDQL"
     gene            complement(828..>1984)
                     /locus_tag="VEP73_08730"
     CDS             complement(828..>1984)
                     /locus_tag="VEP73_08730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT26547.1"
                     /translation="LAERRHLDLSAVRALVLDEADKMLDLGFLPDVERIVRLTPEQRQ
                     TMLFSATMPGDVVTLARRHMRRPTNIRAEGHDEPAPAPLTQQHVFRAHQLDKIEVLAR
                     LLQARNRGLTMVFCQTKRAADQVAEGLERRGFAVATVHGDLGQGQRERALRAFRSGKV
                     DVLTATEVAARGLDVDDVTHVVNYECPDDDMTYVHRIGRTGRAGRAGVAVTFVDWRDM
                     HRWKLINDALSLGMAEPEETYSTSEHLYAELDIPREATGTLPRAMRDRAGLEAEAIED
                     IGETGRIRTPRRAPAGRGSGGRGSAGRGGRTRKSTGDETAQAGGTAGRRRRRTRAGRE
                     IGAAECAGQGQAAPEQAPGQASEQAPRMRRRRRTSARSGNQSEETRRKAS"
BASE COUNT          231 a          733 c          739 g          281 t
ORIGIN      
        1 cagctggtct aggcgggcca gctccgccgt ccggtccggc gcagccagca tgtgcgcggc
       61 catcaggacc aggccgtccg ggtcgttgtc ccgcatccgg cggcccagga acgcgacgat
      121 atgaccgggg acgccggccg cgatggcctg tggctccagg tacgaccgcc agagctcggc
      181 gatcccggcc cgcagtgcgt ccttgtccgg ggcgtctttg tccggggcgt ctttgtccgg
      241 ggcggccgcc cccgggcggc cgccgtcccc ggcgccgagc gaggcgagca tcgcgcgcag
      301 gtcccgcgcc cgggggccgg tcagcgcgcc agggccggag ctcatcagcg tgaacgaggc
      361 gaactcggcg ccggccgcgg ccagcacggc ctcgcggccg atcagcccgc cgtaggagtg
      421 cccgaccagg tgtctcgcgc cggtcgcgtg catgatcgcg aggatgtccg cgccgagggc
      481 ggccgccgcg tagccgcccg gctcgtcggc gcccgcggtc tcgaactggc cgcgcatgtc
      541 gatcgcgacc acgtgccggc tgtcgtcggc cagcaggtcc aagatggcga ggaagtcttc
      601 cttgctgccg gtgtagccgg ggacgagcag agcggcgtcc cgctcgcaca cccccgacgc
      661 gggcgccgcc tccaaggccg cgaacgcccc gcgggcggtg cggatggtgg tacgccgaac
      721 cccgtcaggc agctcgaggg aggtgggagt gctcacagtg gcgaacctta cctcgcgacg
      781 ttgaacagaa cctctaagtc agctcagcga aaaaccagaa accctggtca ggaggccttg
      841 cggcgggttt cctcggactg atttccgctc cgggcggacg tccgcctccg gcggcgcatc
      901 cgcggggcct gctctgaagc ctgcccgggt gcctgctccg gagcggcctg cccctgtccg
      961 gcgcattccg ccgcgccgat ctcgcggccc gccctggtcc ggcggcgccg ccgcccggcc
     1021 gtgccgcccg cctgggctgt ctcgtccccg gtgctcttcc gggtccgccc gccgcggcca
     1081 gcggagccgc ggccaccgga gccccggccc gcgggcgcac gccggggcgt gcggatccgg
     1141 ccggtctcac cgatgtcctc gatggcctcg gcctcgagcc cagcccggtc ccgcatcgcg
     1201 cgggggagcg tcccggtcgc ctcgcgcggg atgtccagct cggcgtagag gtgctcggag
     1261 gtggagtagg tctcctcggg ctcggccatg cccaggctca gcgcgtcgtt gatgagcttc
     1321 cagcggtgca tgtcccgcca gtcgacgaac gtgaccgcga cgccggcccg cccggcccgc
     1381 ccggtccggc cgatgcggtg cacgtaggtc atatcgtcgt ccgggcactc gtagttgacc
     1441 acgtgcgtga cgtcgtccac gtcgagcccg cgggccgcga cctcggtggc ggtgagcacg
     1501 tccaccttgc cgctgcggaa cgcgcgcagg gcgcgctccc gctggccctg gcccaggtca
     1561 ccgtggacgg tggccaccgc gaagccgcgg cgctccagcc cctcggcgac ctggtcggcg
     1621 gcccgcttgg tctggcagaa caccatggtc aggccgcggt tcctggcctg caggagccgc
     1681 gccaggacct cgatcttgtc cagctggtgc gcccggaaca cgtgctgctg ggtgaggggt
     1741 gcgggcgcgg gctcgtcgtg gccctcggcc cggatgttgg tcgggcgccg catgtggcgg
     1801 cgggcgagcg tgacgacgtc gcccggcatg gtcgcggaga acagcatggt ctgccgctgc
     1861 tcaggggtga gccggacgat ccgttccacg tcgggcagga agccgaggtc gagcatcttg
     1921 tcggcctcgt cgagcaccag cgcgcggacc gcgctcaggt ccaggtggcg gcgctcggcc
     1981 aggt
//
LOCUS       DASPYY010000549         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_515676, whole genome shotgun sequence.
ACCESSION   DASPYY010000549 DASPYY010000000
VERSION     DASPYY010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515676"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VEP73_08735"
     CDS             complement(<1..375)
                     /locus_tag="VEP73_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT26548.1"
                     /translation="MSVVDHPPATTPAAAGAAAPPPDATVAVRALWKIFGPREHKILG
                     TPDAELSRAELRAKTGCTAAVCDVSFDVAPGEVFVVMGLSGSGKSTLVRCLTRLIEPT
                     SGEVFIEGEDVRKADQDRLRELR"
     gene            complement(372..1970)
                     /locus_tag="VEP73_08740"
     CDS             complement(372..1970)
                     /locus_tag="VEP73_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HYT26549.1"
                     /translation="MAREAVARGVADDVVCDVVVVGGGTAGAVVAARLSEDPARTVCV
                     VEGGPSDVGDERVLKLRNWINLLGGELDYDYPTVEQPRGNSFIRHARARVLGGCSSHN
                     TMISFLPLPGDFADWERAGATGWGYDAMLPYWQRLETHIQPVARSDRNQLSEDFVDAC
                     SAALGVPVVDDFNAKPFVEAAGFFAVGYDPETGVRSSSSVAYLHPHLDRPNLTLLTET
                     WAHKLDVSAGGEVTGVRVRRGGGTAAVVRARQEYVLCAGAIDTPRLLLLSGVGPADDL
                     RALGIDVVADVPGVGERLLDHPESLILWESTRPLPPSSAMDADAGLFVRRDPAADRPD
                     LMFHLYQLPFTLNTARLGYDVPEHGFAMTPNVPRPRSVGRLWLTSADPAAKPALDFRY
                     FTDPDGYDERTVVDGLRIAREVAAAAPLEAWIAREVAPGPEAQSDEELSAYGRAAHHT
                     VYHPAGTCRMGPDADPLAVTDPELRVRGLRGLRVADASVFPMMTTVNPMVAVLMIGER
                     AAELVAGAPSPTAGSTADSRKDPR"
     gene            complement(2003..>3151)
                     /locus_tag="VEP73_08745"
     CDS             complement(2003..>3151)
                     /locus_tag="VEP73_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26550.1"
                     /translation="VFRYYAGLADKDAGRVVATGSPDAVSRVVYEPIGVCALITPWNY
                     PLLQMSWKVAPALAAGNTVVCKPSEVTPMTTIRLVELLEEAGAPPGVVNLLLGAGGAV
                     GGPLVEHPGVDMVSFTGGLGTGRSIMRAASGEVKRVALELGGKNPNVVFADADLETAV
                     DYALTAAFLHSGQVCSAGARLLVEESVYDAFVAEVAARAERIRLGNGLDERTECGPLV
                     SAEHRDKVERYVAIGREEGARLLAGGRRPSEPELQAGFFYRPTVFADCRRDMRIVQEE
                     VFGPVLTAERFRTEEEAVALGNDTSYGLAGALWTGDAGRAQRVANRLRHGTVWINDYH
                     PYLPQAEWGGFKQSGIGRELGPSGLDEYREAKHIYQNIAPRPQRWFAG"
BASE COUNT          412 a         1135 c         1195 g          409 t
ORIGIN      
        1 gcgcagctcg cgcagccggt cctggtccgc cttgcggacg tcctcgccct cgatgaagac
       61 ctcgccgctg gtcggctcga tgagccgggt caggcagcgg acgagcgtcg acttgccgct
      121 gcccgacagc cccatgacga cgaagacctc gccgggggcc acgtcgaagc tgacgtcgca
      181 cacggccgcc gtgcagccgg tcttcgcgcg cagctcggcc cggctgagct ccgcgtcggg
      241 cgtgcccagg atcttgtgct cccgggggcc gaagatcttc cagagcgccc gtaccgcgac
      301 ggtcgcgtcc gggggcgggg cggccgcgcc cgccgccgcg ggcgtcgtcg cggggggatg
      361 gtcgaccaca ctcatcgtgg atccttcctt gagtcagccg tcgatccagc cgtcggcgac
      421 ggggcgccag cgaccagctc cgcggcgcgc tcgccgatca tcagcacggc gaccatcggg
      481 ttgacggtcg tcatcatcgg gaagaccgag gcatcggcca cgcgcaggcc gcgcaggccg
      541 cggacccgca gctcggggtc ggtgacggcc agcgggtcgg cgtccggtcc catcctgcag
      601 gtaccggcgg ggtggtagac ggtgtggtgc gcggcccggc cgtaggccga cagctcctcg
      661 tcgctctgcg cctccgggcc gggggcgacc tcgcgggcga tccacgcctc gagcggcgcg
      721 gccgcggcca cctcgcgggc gatccgcagc ccgtcgacga ccgtgcgctc gtcgtacccg
      781 tcgggatcgg tgaagtagcg gaagtcgagc gcgggcttgg cggccgggtc ggcgctggtg
      841 agccacagcc gcccgacgct cctcggcctg gggacgttcg gcgtcatggc gaagccgtgc
      901 tccgggacgt cgtagccgag ccgcgcggtg ttcagcgtga aggggagctg gtagaggtgg
      961 aacatgaggt cggggcggtc ggcggccggg tcgcgccgca cgaacaggcc ggcgtcggcg
     1021 tccatcgcgg agctcggcgg cagcgggcgg gtcgactccc acaggatcag cgactccggg
     1081 tggtcgagca ggcgctcccc cacccccgga acgtcggcga cgacgtcgat cccgagcgcg
     1141 cgcaggtcgt cggccgggcc gaccccggag agcagcagga gccggggcgt gtcgatcgcg
     1201 ccggcgcaga gcacgtactc ctggcgggcc cggaccacgg cggcggtgcc gccgccgcgc
     1261 cgcacgcgca cgccggtgac ctcgccgccc gcggagacgt cgagcttgtg ggcccacgtc
     1321 tcggtgagca gcgtcagatt cggccggtcc aggtgcgggt gcaggtaggc gactgacgac
     1381 gacgagcgga cgccggtctc cgggtcgtag ccgacggcga agaagccggc cgcttccacg
     1441 aagggcttcg cgttgaagtc gtcgaccacg ggcacgccga gcgcggccga gcaggcgtcg
     1501 acgaagtcct cggagagctg gttgcggtcg gaccgggcga ccggctggat gtgggtctcg
     1561 agccgctgcc agtagggcag catcgcgtcg tagccccagc ccgtggcacc cgcccgctcc
     1621 cagtcggcga agtcgccggg cagcgggagg aagctgatca tcgtgttgtg cgacgagcag
     1681 ccgccgagca cccgcgccct ggcgtgccgg atgaacgagt tcccgcgggg ctgctcgacc
     1741 gtggggtagt cgtagtcgag ctcgccgccg agcaggttga tccagttgcg gagcttcagc
     1801 acgcgctcgt cgccgacgtc gctggggccg ccctccacga cgcagacggt gcgggccggg
     1861 tcctcgctga gccgcgcggc gacgaccgcg ccggcggtgc cgccgccgac caccacgacg
     1921 tcacagacga cgtcgtcggc gacgccacgg gcgacggcct cacgtgccac ggcagctcac
     1981 ccccgctcgt gcgggcccgt cctcagccgg cgaaccagcg ctgcggcctg ggtgcgatgt
     2041 tctggtagat gtgcttggcc tcacggtact cgtcgaggcc gctcggaccg agctctcgcc
     2101 cgatgccgga ctgcttgaag ccgccccact cggcctgggg caggtaggga tggtagtcgt
     2161 tgatccagac ggtcccgtgg cgcagccggt tggcgacgcg ctgggcccgg cccgcgtcgc
     2221 cggtccacag cgccccggcg agcccgtagg aggtgtcgtt gccgagcgcg acggcctcct
     2281 cctcggtccg gaagcgctcg gcggtgagca caggcccgaa gacctcctcc tggacgatgc
     2341 gcatgtcgcg ccggcagtcg gcgaacaccg tgggccggta gaagaagccg gcctggagct
     2401 ccggctcgct cggccggcgg ccgccggcga gcagccgcgc gccctcctcg cggccgatcg
     2461 cgacgtaccg ctcgaccttg tcgcggtgct cggcggacac cagggggccg cactcggtcc
     2521 gctcgtccag cccgttgccg aggcggatcc gctcggcccg ggccgcgacc tcggcgacga
     2581 acgcgtcgta cacgctctcc tcgacgagca gccgggcgcc ggcggagcag acctggcccg
     2641 agtgcaggaa cgcggcggtg agggcgtagt cgacggcggt ctcgaggtcg gcgtcggcga
     2701 agacgacgtt ggggttcttg ccgccgagct ccagcgcgac ccgcttcacc tccccgctgg
     2761 ccgcccgcat gatcgaccgg ccggtgccga gcccgccggt gaacgacacc atgtcgacgc
     2821 ccgggtgctc gacgagcggc ccgccgaccg ccccgccggc gccgaggagc aggttgacga
     2881 cccccggcgg cgcgccggcc tcctcgagca gctcgacgag ccggatggtg gtcatcgggg
     2941 tgacctcgct gggcttgcag accacggtgt tgcccgcggc cagggcgggc gccaccttcc
     3001 aggacatctg cagcagcggg tagttccagg gcgtgatcag cgcgcagacc ccgatcggct
     3061 cgtagacgac gcggctgacc gcgtcgggcg agccggtggc gacgacccgg cccgcgtcct
     3121 tgtcggccag gccggcgtag tagcggaaga c
//
LOCUS       DASPYY010000550         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_935387, whole genome shotgun sequence.
ACCESSION   DASPYY010000550 DASPYY010000000
VERSION     DASPYY010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935387"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="sat"
                     /locus_tag="VEP73_08750"
     CDS             complement(<1..362)
                     /gene="sat"
                     /locus_tag="VEP73_08750"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.4"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HYT26551.1"
                     /translation="MTATLELAGLPGPHGGALVDRRAADPERLATEAARLPPVALDAR
                     GLADLECLAVGAFSPLTGFLGPDDHAAVVEVGRLADGLAWTLPVALAVPGETLRGAPE
                     RLALTTAEGTPLAVLDVES"
     gene            complement(359..943)
                     /gene="cysC"
                     /locus_tag="VEP73_08755"
     CDS             complement(359..943)
                     /gene="cysC"
                     /locus_tag="VEP73_08755"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HYT26552.1"
                     /translation="MSSGVTVWFTGLPSAGKTTVAELVAKELHDREQAFEVLDGDVVR
                     THLSKGLGFSREDRDENIRRIGFVARLLARHGVTVLVSAISPYRAVRDEVRERVGDFV
                     EVWVATDLATCRARDVKGLYARQARGELRGLTGVDDPYEPPLDPEIVLRTEAEPPEAS
                     AARVVAHLERSGRLEPRPLPAGAVAAGWTGEGRR"
     gene            complement(1009..1905)
                     /locus_tag="VEP73_08760"
     CDS             complement(1009..1905)
                     /locus_tag="VEP73_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT26553.1"
                     /translation="MGAADRTAPPAPAARAAGAELSGLDALERGATAVRGRLARATAA
                     LWPKLAAVALALAAWQLVTWTHWREPWQLPGPGPVLGQLVEDLRQGALERATAITLGR
                     AAVGYALAAVIGTAVGVAVARQRLLRAAVASAITGLQTMPSIAWFPLAILLFQLNEGA
                     ILFVVVLGAAPAIANGVISGVDHVPALLLRAGRALGARGPAFYRHVLLPAALPGYVAG
                     LKQGWAFAWRSLMAGELLVIIAERPSIGVRLQFAREFADAKDILAIMLVILAIGIVVD
                     AVGFGAVERAIRRHRGLTDDTL"
     gene            complement(1892..2710)
                     /locus_tag="VEP73_08765"
     CDS             complement(1892..2710)
                     /locus_tag="VEP73_08765"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26554.1"
                     /translation="MALREREASRAPGGVGVTAGAPAGAPAVTLVGVSKRYGHGAAGV
                     LALDGVSLEIRPGELVCLVGASGCGKSTLLNLVAGLDKPTAGTVSTGGLGTALLFQDA
                     ALFPWLTAGRNVELPLRLRGVGRAERREAAAELLARVGLAEFAGRRPHELSGGMRQRV
                     ALARALAQQADVLLMDEPFGALDAITRDLLHDELERLWQGSRLTVVFVTHNVREAVRL
                     GDRVVLLSSRPGRVAAEFPVDLPRPRRIETPEVAAVAAAVTERLRQEVRRHGRG"
     gene            complement(2710..>3117)
                     /locus_tag="VEP73_08770"
     CDS             complement(2710..>3117)
                     /locus_tag="VEP73_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYT26555.1"
                     /translation="KVLVNEASLWPKGRFVTTHLVVRTEFLDKHPDLVEKLLAGQVEA
                     NELVNRDPAEAQALANAQIQKVTGKALKPAVTAAAWKRLEFTDDPIASSLRTSAAHAE
                     ALGLLKPVKLDGIYDLGPLNRVLASRGQPEVAT"
BASE COUNT          398 a         1140 c         1241 g          338 t
ORIGIN      
        1 gactcgacgt cgagcacggc gagcggggtc ccctcggcgg tcgtgagggc gaggcgctca
       61 ggggcgccgc ggagcgtctc gccaggcacg gcgagcgcga ccggcagggt ccacgccagc
      121 ccgtccgcga gccgcccgac ctccacgacg gcggcgtggt cgtcggggcc gaggaagccg
      181 gtgagcgggg agaacgcgcc gacggcgagg cactcgaggt cggcgaggcc gcgcgcgtcg
      241 agggcgaccg gcgggagccg ggccgcctcg gtggcgagcc gctcggggtc ggcggcccgc
      301 cggtcgacca gggccccgcc gtgcgggccg ggcaggccgg cgagctccag ggtggcggtc
      361 accggcgccc ctcccccgtc cacccggccg ccacggcgcc ggccggcagt ggccgcggct
      421 cgagacggcc gctgcgctcg aggtgggcga ccacccgcgc ggccgacgcc tccggcggct
      481 cggcctcggt gcggagcacg atctcggggt cgagcggcgg ctcgtagggg tcgtccacgc
      541 cggtgaggcc gcgcagctcg ccgcgggcct gcctggcgta caggcccttg acgtcgcggg
      601 cgcggcaggt ggcgaggtcg gtggcgaccc agacctcgac gaagtcgccg acgcgctcgc
      661 ggacctcgtc gcggaccgct cggtagggcg agatcgccga gacgagcacg gtgacgccgt
      721 ggcgggcgag cagccgggcg acgaacccga tgcggcggat gttctcgtcg cggtcctcgc
      781 gggagaagcc gaggcccttg gacaggtggg tgcgcaccac gtcgccgtcg agcacctcga
      841 acgcctgctc gcggtcgtgg agctccttgg cgacgagctc cgcgactgtc gtcttgccgg
      901 ccgaggggag gccggtgaac cacacggtga cgccggagga catgcggagg gtcctttcgc
      961 tggggacgaa ggtcaggggc cggggtcggt gtcaggggca gagatgtgtc agagggtgtc
     1021 gtcggtcagg ccgcggtggc gccggatcgc gcgctcgacc gcaccgaacc cgaccgcgtc
     1081 gacgacgatg ccgatcgcga ggatcacgag catgatggcg aggatgtcct tggcgtcggc
     1141 gaactcgcgg gcgaactgga gtcgcacgcc gatcgacggg cgctccgcga tgatgaccag
     1201 cagctccccc gccatgaggc tccgccaggc gaacgcccag ccctgcttga gcccggcgac
     1261 gtagccgggc agggcggccg ggagcagcac gtgccggtag aacgcgggcc cgcgcgcgcc
     1321 cagcgcccgg ccggcgcgca gcagcagggc cgggacgtgg tcgacgccgc tgatgacgcc
     1381 gttggcgatc gcgggcgcgg cgccgagcac gacgacgaac aggatcgcgc cctcgttgag
     1441 ctggaacagc aggatcgcga gcgggaacca ggcgatcgac ggcatcgtct gcaggccggt
     1501 gatggccgag gcgaccgctg cgcgcaggag ccgctggcgc gcgacggcca ccccgaccgc
     1561 ggtcccgatc acggcggcga gcgcgtagcc gaccgcggcg cggcccagcg tgatcgcggt
     1621 cgcccgctcg agcgcgccct ggcggaggtc ctcgacgagc tggccgagga ccgggcccgg
     1681 cccgggcagc tgccagggct cccgccagtg cgtccaggtg acgagctgcc aggccgcgag
     1741 cgcgagcgcg accgcggcga gcttgggcca cagcgccgcg gtcgcgcgcg cgaggcggcc
     1801 gcggaccgcc gtcgcgcccc gctcgagcgc gtcgagcccg gacagctcgg cgccggcggc
     1861 ccgtgcggcg ggcgccggcg gcgcggtccg gtcagccgcg cccatggcgg cgcacctcct
     1921 gccgcagccg ctcggtgacg gcggccgcga ccgcggcgac ctcgggcgtc tcgatgcggc
     1981 ggggccgggg caggtcgacc gggaactcgg ccgccacccg cccggggcgg ctcgacagca
     2041 ggaccacacg gtcgccgagg cggaccgcct cgcggacgtt gtgggtgacg aacacgacgg
     2101 tcaggcggct cccctgccac agccgctcga gctcgtcgtg gagcaggtcg cgggtgatcg
     2161 cgtcgagcgc gccgaagggc tcgtccatga gcagcacgtc ggcctgctgg gcgagggcgc
     2221 gggcgagggc gacccgctgg cgcatgccgc ccgacagctc gtggggtcgc cggccggcga
     2281 actcggccag ccccacccgg gcgagcagct cggccgctgc ctcgcgccgc tcggccctgc
     2341 cgacgccccg caggcgcagc ggcagctcga cgttgcgccc ggcggtcagc cacgggaaca
     2401 gggcggcgtc ctggaacagc agggcggtcc cgagcccgcc ggtgctcacc gtccccgccg
     2461 tcggcttgtc cagcccggcg acgagattca gcagggtgct cttgccgcac ccggacgcac
     2521 cgaccaggca gacgagctcg ccgggccgga tctcgagcga caccccgtcg agggccagca
     2581 ccccggcggc gccgtggccg taccgcttgg acacgcccac gagcgtgacc gcgggggcgc
     2641 cggccggcgc ccccgcggtc acgccgaccc cgcccggagc gcgcgaggcc tcgcgctcac
     2701 gcaacgccat caggtcgcca cctccggctg gccgcgggag gccagcacgc ggttgagcgg
     2761 gccgaggtcg tagatgccgt caagcttgac cggcttgagc aggccgagcg cctccgcgtg
     2821 ggccgccgag gtccgcagcg acgaggcgat cgggtcgtcg gtgaactcga ggcgcttcca
     2881 cgcggcggcg gtcaccgcgg gcttgagggc cttgccggtc accttctgga tctgggcgtt
     2941 ggcgagcgcc tgcgcctccg ccgggtcgcg gttgacgagc tcgttcgcct cgacctggcc
     3001 ggcgaggagc ttctcgacga ggtcggggtg cttgtcgagg aactccgtcc gcaccacgag
     3061 gtgcgtggtg acgaaccgcc ccttcggcca caggctggcc tcgttcacga gcacctt
//
LOCUS       DASPYY010000551         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1920746, whole genome shotgun sequence.
ACCESSION   DASPYY010000551 DASPYY010000000
VERSION     DASPYY010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920746"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1210
                     /locus_tag="VEP73_08775"
     CDS             104..1210
                     /locus_tag="VEP73_08775"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HYT26556.1"
                     /translation="MPENELARVFEASGPFASIYLTTSGDVENAAQRVALRWKNLRGE
                     LARAGVPEATLEAIDPLVEGSHTAGACLAVITAVDGVVWSENLPDPPPREVLVRHGPV
                     PHVLPLLARQQALVPHVAVLTDRTGADMAARVPEQRGQVEHVEGAWQGPHLHRSSPGG
                     WSQQRYQHRAEVMWERNASEVAEALAKLVDHVRPRFVAAAGDVRALQLLREECPKRVR
                     DLMEVVGGEYASIDAVFDKADELAAGVAEGDTAALLERFAAERAGALAADGVAATIGA
                     LPKGQVDTLLVRDDPDDERTAWFGEAPGLVAIDPATLAAIGVATPLEGRLVDVAVRAA
                     LGTGAQVRVLDGGVGGPERPGPAEGLGALFRFAG"
     gene            complement(1262..1489)
                     /locus_tag="VEP73_08780"
     CDS             complement(1262..1489)
                     /locus_tag="VEP73_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT26557.1"
                     /translation="MLAAPPPAEEPEDLLLLLPGLVASLPELERRVVLLRYCQDLSQD
                     EIAARVGYSQMHVSRLLRRALDRLRDQLLIA"
     gene            complement(1496..2326)
                     /locus_tag="VEP73_08785"
     CDS             complement(1496..2326)
                     /locus_tag="VEP73_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT26558.1"
                     /translation="MRRIENAKALDGPGEQLGEAVATATRPRLVKNALSGTWLGHRLH
                     PGLVPLPIGMWSGALLLDFVGTRSARSGADVLVGAGVVAALPSAAAGLSDWSDTFGGA
                     RRVGLVHATANSVALACYTGSLVARLLGRRKPGVALALLGAAAVGVGGYLGGHLSYVQ
                     GVGVERKAFEDGPTEWTPVLGADELAEGAPTAVRAGDTVVMLYREDATVHALAERCTH
                     EGGPLHEGSFRDGCVTCPWHGSTFRLRDGGVVRGPAASSQALYETRTVGGKLEVRRVT
                     "
     gene            2760..>3400
                     /locus_tag="VEP73_08790"
     CDS             2760..>3400
                     /locus_tag="VEP73_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26559.1"
                     /translation="MVALLALAVTAAYLTWRAVATVEIAVWWLSLPLYVLELHAAVGL
                     GLYTLALWDVQAGPRARPVRETRLRVAVLIPTWNESLEVLAPTIAAAVALRPAHETWV
                     LDDSDRPPVAGLAGELGAYYLRRADRSHAKAGNLNHALEVVDADVVAVLDADHVARPA
                     FLTSTLGYFDDERVALVQTPQDFYNLESFEHSRTAARDGFNEQSLFYRAILPG"
BASE COUNT          427 a         1292 c         1259 g          422 t
ORIGIN      
        1 aggacatggc gccagcggcc caggacatgg cgccagcggc ccaggacatg gcgccagcgg
       61 cccaggacat ggcgccagcg gcccaggaca tgaggaggca accgtgcccg agaacgagct
      121 cgcccgggta ttcgaggcca gcgggccctt cgcctcgatc tacctgacga ccagcggaga
      181 cgtggagaac gccgcccagc gcgtcgccct gcggtggaag aacctgcgcg gcgagctcgc
      241 ccgggccggc gtgcccgagg cgaccctcga ggcgatcgac ccgctcgtcg aaggcagcca
      301 caccgccggc gcatgcctcg cggtcatcac ggccgtcgac ggcgtcgtgt ggtcggagaa
      361 cctcccggac ccgcctcccc gcgaggtcct cgtccgtcac gggcccgtgc cgcacgtcct
      421 gccgctgctc gcgcggcagc aggcgctcgt cccccacgtc gcggtcctca ccgaccgcac
      481 cggcgccgac atggcggccc gcgtccccga gcaacgcggc caggtcgagc acgtcgaggg
      541 cgcctggcag ggcccgcacc ttcaccggtc cagcccgggc ggctggtccc agcagcgcta
      601 ccagcaccgc gccgaggtca tgtgggagcg caacgcgagc gaggtcgccg aggcgctcgc
      661 caagctcgtc gaccacgtgc ggccgcgctt cgtcgcggca gccggcgacg tgcgtgccct
      721 ccagctcttg cgcgaggagt gcccgaagcg ggtgcgcgac ctcatggagg tcgtcggcgg
      781 ggagtacgcc tcgatcgacg ccgtgttcga caaggccgac gagctcgccg cgggcgtggc
      841 cgagggcgac acggccgccc tgctcgagcg gttcgcggcg gagcgcgcgg gcgcgctggc
      901 cgccgacggc gtcgccgcga cgatcggcgc cctgcccaag ggccaggtcg acacgctgct
      961 ggtccgcgac gaccccgacg acgagcgcac cgcctggttc ggggaggcgc ccgggctggt
     1021 cgccatcgac cccgcgacgc tcgcggcgat cggcgtcgcc acccccctgg agggccggct
     1081 ggtcgacgtc gccgtccgtg ccgccctcgg gaccggcgcc caggtccggg tgctcgacgg
     1141 cggggtgggc ggccccgagc ggccggggcc cgcggagggg ctcggcgcgc tgttccgctt
     1201 cgccggctga gacgccgccg gcgaagcggg cgaggcgccc tcgaccagcc ggccgtgccg
     1261 gtcaggcgat gaggagctgg tctcggagcc ggtcgagggc gcggcgcagc aggcgggaga
     1321 cgtgcatctg ggagtagccg acccgggcgg cgatctcgtc ctggctcagg tcctggcagt
     1381 agcgcagtag caccacgcgc cgctcgagct cgggcaggga ggcgaccagc ccgggcagca
     1441 gcagcagcag gtcctcgggc tcctcggcgg gcggcggcgc ggccagcagg tcggctcagg
     1501 tgacccggcg cacctcgagc ttgccgccca cggtccgcgt ctcgtacagg gcctgggagg
     1561 aggccgccgg gccgcggacg accccgccgt cgcgcaggcg gaaggtgctg ccgtgccagg
     1621 ggcaggtcac gcagccgtcc cggaacgagc cctcgtggag cgggccgccc tcgtgggtgc
     1681 agcgctcggc cagggcgtgc acggtggcgt cctcccggta gagcatgacg accgtgtcgc
     1741 ccgcccggac ggccgtcggc gcgccctcgg ccagctcgtc ggccccgagc accggggtcc
     1801 actcggtcgg cccatcctcg aacgccttgc gctcgactcc cacgccctgc acgtaggaga
     1861 ggtggccgcc caggtagccg ccgacgccga cggccgcggc cccgaggagc gcgagggcga
     1921 cccctggctt gcgccggccg agcaggcggg ccacgagcga cccggtgtag caggccagcg
     1981 cgaccgagtt ggcggtcgcg tggacgagcc ccacgcgcct ggccccgccg aaggtgtccg
     2041 accagtccga caggccggcc gccgcgctcg gcagcgcggc gacgacgccg gcgccgacca
     2101 gcacgtcggc accggaccgg gccgagcggg tgccgacgaa gtcgagcagc aaggcgccac
     2161 tccacatgcc gatcggcagc ggcaccaggc ccgggtgcag ccggtgcccc agccaggtcc
     2221 cgctcagggc gttcttcacc aggcggggtc gggtggcggt ggccaccgcc tcgccgagct
     2281 gctcgcccgg cccgtcaagc gccttggcgt tctcgatgcg cctcatgagg gactcgattg
     2341 gcgttggcac ggcaacctcc ggggtcggcg ggcgctctgg cactcccggt tgccccgcaa
     2401 gggtgcgcgc aaacgttgtg caagcccggc ctcaacctcg cgtcgcgccc gccgatcttc
     2461 gatctctgga cgctgtctcg agatcagggg tgagcatggt gcgcaagcgg gacaggacgg
     2521 gcaagcgctg gggcgtctct ttcccgttcg gcccgcctct ccctgtcccg atcggcacgc
     2581 ctttccctgt cccgtccggc acgccgaggc gtgcctccga acggcgtgcc tccgtacggc
     2641 gtgcctccgt acggcgtgcc tccgcacggc gtgcctccgc cgccgcccgg ccggagctgc
     2701 cctcgttccc cgacggcagc gccccggcgg gctcgggcgt ccgcgaccgg gtcgcgcgcg
     2761 tggtcgccct gctcgcgctg gccgtcacgg cggcgtacct gacctggcgg gccgttgcca
     2821 cggtcgagat cgcggtgtgg tggctgtcgc tcccgctcta cgtcctggag ctgcacgcgg
     2881 cggtcgggct gggcctgtac accctggcgc tctgggacgt gcaggccggt ccgcgggcac
     2941 ggccggtccg ggagacccgg ctgcgggtgg cggtgctgat cccgacctgg aacgagtcgc
     3001 tcgaggtgct ggcgccgacc atcgcggccg cggtggcgct gcggcccgcc cacgagacct
     3061 gggtcctcga cgactccgac cggccgcccg tcgccggtct ggccggtgag ctgggcgcct
     3121 actacctgcg tcgcgccgat cgcagccatg ccaaggctgg caacctgaac cacgccctcg
     3181 aggtcgtcga tgccgacgtg gtggccgtgc tggacgccga ccacgtcgcc aggcccgcct
     3241 ttctcaccag taccctcggc tacttcgacg acgaacgggt cgcgctcgtg cagaccccgc
     3301 aggacttcta caacctcgag tcgttcgagc acagccgcac ggccgcaagg gacgggttca
     3361 acgagcagtc gctgttctac cgggccatcc tgccaggcaa
//
LOCUS       DASPYY010000552         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1778607, whole genome shotgun sequence.
ACCESSION   DASPYY010000552 DASPYY010000000
VERSION     DASPYY010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778607"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VEP73_08795"
     CDS             complement(<1..315)
                     /locus_tag="VEP73_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26560.1"
                     /translation="MIRQHSQAIVPNIPDPGPEGPADHLVVVAAPTVVGGAAAVVAPV
                     VAADLVLVGAAVAGGLVVVGAAVVVGAAVVFGATVVVGATVVLGGAVVLGGAVVLGGA
                     VVA"
     gene            397..>1633
                     /gene="thrS"
                     /locus_tag="VEP73_08800"
     CDS             397..>1633
                     /gene="thrS"
                     /locus_tag="VEP73_08800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYT26561.1"
                     /translation="MQEIQVKLPDGAVRQVPAGTTPAELLDTTGAVAALVDDEPWDLG
                     RGLDHDAAVAALPVASEQGRAVLRHSAAHVLAQAVTDLYPGARYAIGPPIEDGFYYDF
                     DVPEPFTPDDLERIEGRMRQIVAEGQPFRRVELSREEGLRLFADQPYKREIIEAVDES
                     EGAGQGVSVYRNDGFADLCRGPHVPSTSAIPAFRLMRTAGAYWRGDESRPQLQRIYGT
                     AWESDAALAAYLHRLEEAERRDHRRLGQELDLFHFPPEIGSGLPVFHPKGGLIRKLME
                     DYSRAEHEAAGYSFVWTPHLARSTLFELSGHLQWYAEGMYPPMELEGAQYYPKPMNCP
                     MHILIYKSRGRSYRELPLRLFEFGTVYRFERSGVVHGLTRVRGITQDDAHIFCTREQL
                     APELASLLDFVLRLLRTFGL"
BASE COUNT          237 a          651 c          542 g          203 t
ORIGIN      
        1 ggcgacgacc gcgccgccga ggaccaccgc gccgccgagg accaccgcgc cgccgaggac
       61 caccgtggcg cccaccacga ccgtggcgcc gaagaccacc gcggcgccca cgaccaccgc
      121 cgcgccgacc accacgaggc cgccggcgac ggccgccccg acgagcacga ggtcggcggc
      181 gaccaccggt gcgaccaccg cggcggcgcc gcccacgacc gtcggggcgg cgaccacgac
      241 caggtgatcg gccgggccct cgggtcccgg gtcggggata ttggggacga tcgcctggga
      301 gtgctggcgt atcatccggc cgttgccacg tcgtccagcc gggtcgtgca aacccaggtc
      361 gtgcaaaccc aggtcgtcca tccaggaggc agccccgtgc aagagatcca ggtcaagctc
      421 cccgacggcg ccgtgcggca ggtccctgcc ggcacgaccc cggccgagct gctggacacc
      481 acgggcgccg tggccgcgct ggtcgacgac gagccctggg acctcggccg cggcctcgac
      541 cacgacgccg ccgtcgccgc cctgcccgtg gccagcgagc agggccgggc cgtgctgcgc
      601 cactctgccg cccacgtgct cgcccaggcc gtgaccgacc tgtacccggg cgccaggtac
      661 gcgatcgggc cgccgatcga ggacggcttc tactacgact tcgacgtgcc cgagccgttc
      721 accccagacg acctcgagcg catcgagggc cgcatgcgcc agatcgtcgc agagggccag
      781 ccgttccggc gcgtcgagct ctcccgcgag gagggcctcc ggctcttcgc cgaccagccc
      841 tacaagcgcg agatcatcga ggcggtggac gagtccgagg gcgcaggcca gggggtgtcc
      901 gtctaccgca acgacggctt cgccgacctc tgccgcggcc cgcacgttcc cagcaccagc
      961 gccatcccgg cgttcaggct gatgcgtacc gccggcgcct actggcgcgg cgacgagtcg
     1021 cgcccgcagc tccagcggat ctacggcacc gcctgggagt ccgacgccgc gctggccgcg
     1081 tacctgcacc ggctcgagga ggccgagcgc cgcgaccacc gccgcctcgg ccaggagctc
     1141 gacctgttcc acttccctcc cgagatcggc agcgggctgc ccgtgttcca ccccaagggc
     1201 ggcctcatcc gcaagctcat ggaggactac tcccgggccg agcacgaggc ggccggctac
     1261 tccttcgtgt ggaccccgca cctggccagg tcgaccctgt tcgagctctc cggccacctg
     1321 cagtggtacg cggagggcat gtacccgccc atggagctgg agggcgccca gtactacccg
     1381 aagccgatga actgcccgat gcacatcctg atctacaagt cgcggggccg ctcctaccgc
     1441 gagctgccgc tgcgcctgtt cgagttcggg accgtgtacc gcttcgagcg ttcgggcgtc
     1501 gtccacgggc tcacccgggt gcgcggcatc acccaggacg acgcccacat cttctgcacc
     1561 cgcgagcagc tcgcccccga gctcgccagc ctgctcgact tcgtcctccg gctgctgcgc
     1621 accttcgggc tga
//
LOCUS       DASPYY010000553         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2762029, whole genome shotgun sequence.
ACCESSION   DASPYY010000553 DASPYY010000000
VERSION     DASPYY010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762029"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..699
                     /locus_tag="VEP73_08805"
     CDS             235..699
                     /locus_tag="VEP73_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYT26562.1"
                     /translation="MTRTAPGGCAPWNAGSTPATALLPFYAGLRLSELIALDLPDIKL
                     SARKGLVEERPNWPGADTPALLLSQRSRRLTARAADDILGAIAEEANLSIDNFTPHVL
                     RHTFGTRLVREGHDLVLVAELMGYARLEQVRRYSLPTQADRQRAIDSLLTDA"
     gene            1011..>2421
                     /locus_tag="VEP73_08810"
     CDS             1011..>2421
                     /locus_tag="VEP73_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT26563.1"
                     /translation="MDGHGGALARELEVERAQLARARAYLAAMRRHAGSLDASGGDEI
                     AESIVRARLGLRVAALADDPTVPLFFGRIDYDGSAELAGERFHIGRRHIHDELMDPVV
                     IDWRAAVARPFYRASPQDPWGLVRRRRFGFADGELTSFQDERFEEGADQSVSEAALRA
                     EIERPRVGPMRDIVATIQPEQDDLVRASLGETICIQGGPGTGKTAVGLHRAAFLLYEY
                     RDRLRTGDVLVVGPSPAFMAFISQVLPGLGEVDVTQRTIDELVASVPLRGADAPSAAA
                     VKADARMAAVLERAVSGRVRPPLEPLAIPFATSFVQLSSARLRAMVAALLDRDLPYAA
                     GRELLWEQLGRACQSEAEQAGHTGYESVDDAVKAVRRSRAARRVVDRAWPALQPTRLL
                     FDLLSDEAALRNAASGILDDVEQKELLWPRRPQSRASAPWTAADALLLDELAALIDPP
                     RSYGHVVIDEAQDLSPMQLR"
BASE COUNT          353 a          933 c          825 g          310 t
ORIGIN      
        1 agtacctcgc ctgggtagcc gacgccgacc tcgacggcga cccgctcacc cagccagccg
       61 cccgcgacgg cgccgtgcgc gacgaccgcg cccacctgca gaccgtgctg cgccgcaagc
      121 cgaccacggt caacctggcc ctggccgccc tggtcgactt ctacacccgg ctcggcctcg
      181 gcccgcccga ggcccgtcgc ctcgacctcc ccaagcaggc gccacgcgcc ctcagtgaca
      241 aggacagcac ccggtggctg cgcaccgtgg aacgctggct caacccccgc gaccgcgcta
      301 ctacccttct acgccgggct gcggctgtcc gagctgatcg ccctggacct gcccgacatc
      361 aagctgtcgg cccgcaaagg gctggtggag gaacgcccca actggcccgg cgccgacacc
      421 ccggccctgc tgctcagtca acgcagccgc cgactcaccg cccgcgcggc cgacgacatc
      481 cttggcgcca tcgcagagga ggccaacctc agcatcgaca acttcacccc ccacgtactg
      541 agacacacct tcggaacccg cctcgtccgg gaaggccatg acctcgtgct cgtcgccgaa
      601 ctcatgggct acgcccgcct ggaacaagta cgccgctaca gcctgcccac ccaagccgac
      661 cggcaacgag ccatcgatag ccttctcacc gatgcctgac cagcgtatct aaagatctac
      721 gggcgtattc tcctcatccc cgtgagcgtc cggcaccgcc ccgtctccct ctctaaagac
      781 cgataacaag taagatttca gacaccatcg caaaccccac ccagccaaaa cgagagcccg
      841 accacgcggc caggatcatc aaggtcacgt gagccagctg gtccgataac ggctcgcggt
      901 cgggtcggcc gactcggtag catggccggc tggtgcggga cttcgggccg accgccgcgg
      961 cggggccttg ccggagtctc tcgacgcgac cggctcgggg tggtcggatc atggacgggc
     1021 acggcggcgc gctcgcgcgc gagctggagg tggagcgcgc ccagctcgcg cgggcacggg
     1081 cgtacctggc ggcgatgcgg cggcatgcgg ggtcgctcga cgcgagcggc ggggacgaga
     1141 tcgccgagag catcgtgcgg gcgcggctcg gcctccgcgt tgccgcgctg gccgacgacc
     1201 cgaccgtgcc gctgttcttc ggccggatcg actacgacgg gagcgccgag ctcgccggcg
     1261 agcggttcca catcgggcgc cgccacatcc acgacgagct gatggacccg gtggtgatcg
     1321 actggcgcgc ggcggtcgcc cggccgttct accgcgccag cccgcaggac ccgtgggggc
     1381 tcgtgcgccg ccgccggttc gggttcgcgg acggcgagct cacctccttc caggacgagc
     1441 gcttcgagga gggcgcggac cagtccgtct cggaggcggc gctccgggcc gagatcgagc
     1501 ggccccgcgt cggcccgatg cgcgacatcg tcgcgacgat ccagcccgag caggacgacc
     1561 tcgtgcgcgc gtccctcggg gagacgatct gcatccaggg cggtccgggg acgggcaaga
     1621 ccgcggtcgg gctccaccgg gccgccttcc tcctctacga gtaccgcgac cggctgcgca
     1681 ccggcgacgt cctggtcgtc ggcccgagcc cggcgttcat ggcgttcatc agccaggtgc
     1741 tgcccgggct cggggaggtc gacgtcaccc agcggacgat cgacgagctg gtcgccagcg
     1801 tgccgctgcg cggcgcggac gcgccctcgg ccgccgcggt caaggccgac gcgcggatgg
     1861 ccgcggtcct cgagcgggca gtgtccgggc gcgtccgccc gccgctcgag cccctggcga
     1921 tcccgttcgc cacgagcttc gtccagctct cctcggccag gctccgggcc atggtggcgg
     1981 ccctgctcga ccgggacctt ccctacgcgg caggccgcga gctcctctgg gagcagctcg
     2041 gccgcgcctg ccagagcgaa gcggagcagg ccgggcacac cgggtacgag tcggtcgacg
     2101 acgcggtcaa ggcggtccgc cggtcgaggg cggcgaggcg ggtcgtcgac cgcgcgtggc
     2161 cggcgctcca gccgacgcgg ctcctgttcg acctgctgag cgacgaggcg gcactccgca
     2221 acgccgcaag cgggatcctg gacgacgtcg agcagaagga gctcctctgg ccccggcggc
     2281 cgcagagccg cgcctcggcg ccgtggacgg ccgccgacgc gctgctgctc gacgagctcg
     2341 cggccctgat cgacccgccc cggtcctacg gccacgtggt gatcgacgag gcccaggacc
     2401 tgtcgcccat gcagctccgg g
//
LOCUS       DASPYY010000554         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1638611, whole genome shotgun sequence.
ACCESSION   DASPYY010000554 DASPYY010000000
VERSION     DASPYY010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638611"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /gene="nuoF"
                     /locus_tag="VEP73_08815"
     CDS             <1..1026
                     /gene="nuoF"
                     /locus_tag="VEP73_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYT26564.1"
                     /translation="FKDMELLERDPHALVEGVIVSAYAIGCRTAFIYLRGECGFAGRR
                     LADALADAYDRGLLGRDVRGSGFDLDVVLHRGAGAYICGEETALLSSLEGQRGQPKLR
                     PPFPAVEGLYRAPTTVNNVETIATVPWILRKGVDWFRGMGTEKSPGPKLVSVSGDVRN
                     PGNFEFPMGTPAREIIMEAAGGMLEGKGLKAWTPGGSSTPMLTADHLDVTMDFEAIQA
                     AGSLLGTAAVIVMSEDVCIVRACLNFTEFYAHESCGKCTPCREGTDWMRKILTRLETG
                     QGRQEDLKTLQDVCDSIFGRSFCALGDGATSPIVSGLKYFRDEFTQHVEGHGCPFPGA
                     RVAVGAH"
     gene            1040..>3133
                     /locus_tag="VEP73_08820"
     CDS             1040..>3133
                     /locus_tag="VEP73_08820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HYT26565.1"
                     /translation="MADKPSTVKVTIDGNELEVPPGTLVIRAAEQVGIVVPRFCDHRL
                     LAPLGACRQCLVEVEGQRKPLTACTTTVTDGMVVKTQETSEIARAGQEGTLELLLINH
                     PLDCPMCDKGGECPLQDQTLAHGPGETRFVETKRHYQKPIKISETVLLDRERCVLCAR
                     CTRFSSEVAGDPFIELFERGALEQVAIFDDEPYESYFSGNVVQICPVGALTSPSYRFQ
                     ARPFDLTSADGVCNHCAAGCNLRVDSRRGLVTRQLARDNDEVNEAWNCDRGRYGFAWA
                     QDPRRVLVPNVSEDGNLRTASWSEALRRAADGIRAARGKGVGVLVGGHLTDQDAYALQ
                     KLARVTFGTNNVDARSWPGSEAEAAAVARVAGRSGPAYADLEAAAAVVVVDLDPHEES
                     PILFLRLRKAALRKGVPVVTVGPRSGRLAEFATVVPTLPGGEADALDELARGEGDAGR
                     VVAAATGPRDGEGGPQRARKGPVVVLAGWRAAATGSLGAAVRFADGLGASFAWVPRRA
                     GDRGALDVGARADLLPGGRAVADPAARGALAEIWGAELPEAPGLDGPAMVRAAADGEL
                     GVLLLAGVDLVRDYGPRELAERALANAFVVAVDHSVNDTTRHADVLLPGVVHAETEGT
                     FTDWEGRVQHQSPAVPVGGAAQTVWELADQLSLRLKVDLGLATWGQVAAETKRFRARP
                     AQAAAAAVPDAPQAAP"
BASE COUNT          416 a         1199 c         1124 g          394 t
ORIGIN      
        1 ttcaaggaca tggagctcct cgagcgcgac ccccacgccc tggtcgaggg cgtgatcgtc
       61 tccgcgtacg ccatcggctg ccgcaccgcc ttcatctacc tgcgcgggga atgcggcttc
      121 gccgggcggc ggctcgccga cgccctcgcc gacgcctacg accggggtct cctgggccgg
      181 gacgtccggg ggtccgggtt cgacctcgac gtggtgctgc accggggcgc cggggcgtac
      241 atctgcggcg aggagaccgc cctcttgtcc tcgctcgagg gccagcgggg ccagcccaag
      301 ctgcgcccac cgttcccggc cgtcgagggc ctctaccggg caccgaccac ggtgaacaac
      361 gtcgagacga tcgccaccgt cccctggatc ctccgcaagg gcgttgactg gttccgcggc
      421 atgggcaccg agaagtcgcc cggccccaag ctggtcagcg tctcgggcga cgtccgcaac
      481 ccgggcaact tcgagttccc catgggcacg ccggcgcggg agatcatcat ggaggccgcc
      541 ggcgggatgc tcgagggcaa ggggctcaag gcgtggacgc caggcggctc ctcgaccccg
      601 atgctcaccg ccgaccacct cgacgtcacc atggacttcg aggcgatcca ggcggcgggc
      661 agcctgctcg gcacggccgc ggtgatcgtg atgtccgagg acgtctgcat cgtccgcgcc
      721 tgtctcaact tcaccgagtt ctacgcccac gagtcgtgcg gcaagtgcac gccctgccgc
      781 gagggcaccg actggatgcg gaagatcctg acccggctcg agaccggcca gggccggcag
      841 gaggacctga agacgctgca ggacgtctgc gactcgatct tcggccgctc cttctgcgcc
      901 ctcggcgacg gggccacgag cccgatcgtc tccgggctca agtacttccg ggacgagttc
      961 acccagcacg tcgagggcca cggctgcccg ttcccgggcg cccgggtggc cgtcggcgcg
     1021 cactaggaga caggtggcca tggccgacaa gccgagcacc gtcaaggtca cgatcgacgg
     1081 caacgaactc gaggtgccgc cgggcaccct cgtgatccgc gccgccgagc aggtcgggat
     1141 cgtcgtcccg cgcttctgcg accaccggct gctcgctccc ctcggcgcct gccgccagtg
     1201 cctcgtcgag gtcgagggcc agcgcaagcc gctgaccgcc tgcaccacca cggtgacgga
     1261 cgggatggtg gtcaagaccc aggagacgag cgagatcgcc cgcgccggcc aggagggcac
     1321 cctcgagctg ctgctgatca accacccgct cgactgcccg atgtgcgaca agggcggcga
     1381 gtgcccgctg caggaccaga ccctcgccca cggtcccggc gagacccggt tcgtcgagac
     1441 caagcggcac taccagaagc cgatcaagat ctccgagacc gtgctgctcg accgggagcg
     1501 gtgcgtgctc tgcgcccgct gcacccgctt ctccagcgag gtcgccggcg acccgttcat
     1561 cgagctgttc gagcggggcg cgctcgagca ggtggcgatc ttcgacgacg agccgtacga
     1621 gagctacttc tccggcaacg tcgtccagat ctgccccgtg ggcgcgctca cctcgccgtc
     1681 ctaccgcttc caggcccgcc cgttcgacct gacctcggcc gacggcgtct gcaaccactg
     1741 cgccgccggc tgcaacctgc gcgtcgactc gcgccgcggg ctcgtcaccc ggcagctcgc
     1801 ccgcgacaac gacgaggtca acgaggcgtg gaactgcgac cggggtcgct acggcttcgc
     1861 ctgggcccag gacccgagga gggtgctcgt cccgaacgtc agcgaggacg gcaacctgcg
     1921 caccgcgtcc tggtcggagg ccctgcgccg ggccgccgac ggcatcaggg cggcccgcgg
     1981 caagggcgtc ggggtgctgg tcggcggcca cctgaccgac caggacgcgt acgcgctgca
     2041 gaagctcgcc cgggtgacct tcggcaccaa caacgtcgac gcccgcagct ggccgggcag
     2101 cgaagcggag gccgccgccg tggccagggt cgccggccgg tccggccccg cctacgccga
     2161 cctcgaggcg gccgcggcgg tcgtcgtggt cgacctcgac ccgcacgagg agtcccccat
     2221 cctgttcctc cgcctccgca aggcggccct gcgcaagggc gttccggtcg tgaccgtcgg
     2281 cccccggtcc ggccgcctgg ccgagttcgc caccgtcgtg ccgaccctgc ccggcgggga
     2341 ggccgacgcg ctcgacgagc tcgcgcgtgg cgagggggac gccggccgcg tcgtcgccgc
     2401 cgcgaccggg ccacgcgacg gcgagggggg gccccagcgg gcgcggaagg ggccggtcgt
     2461 cgtgctcgcc ggctggcggg ccgccgcgac cggctcgctc ggcgccgccg tccggttcgc
     2521 ggacgggctc ggcgccagct tcgcctgggt gccgaggcgg gcgggggacc ggggcgcgct
     2581 cgacgtcggc gcccgggccg acctcctgcc cggcggccgc gcggtggccg acccggcggc
     2641 ccgtggcgca ctggccgaga tctggggcgc ggagctgccc gaggcccccg gcctcgacgg
     2701 cccggccatg gtcagggccg ccgccgacgg cgagctcggc gtgctgctgc tcgccggcgt
     2761 cgacctggtg cgcgactacg gcccgcggga gctggccgag cgcgccctcg ccaacgcctt
     2821 cgtggtggcg gtcgaccact cggtcaacga cacgacgcgg cacgccgacg tgctcctgcc
     2881 cggcgtggtc cacgccgaga ccgagggcac gttcaccgac tgggaggggc gggtccagca
     2941 ccagagcccc gccgtccccg tgggcggcgc cgcccagacc gtctgggagc tcgccgacca
     3001 gctgtcgctc aggctcaagg tcgacctggg cctcgcgacc tggggccagg tcgccgccga
     3061 gaccaagcgc ttccgggccc ggccggcaca ggccgccgcg gccgcggtcc ccgacgcgcc
     3121 gcaggcggcc cct
//
LOCUS       DASPYY010000555         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2621169, whole genome shotgun sequence.
ACCESSION   DASPYY010000555 DASPYY010000000
VERSION     DASPYY010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621169"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VEP73_08825"
     CDS             <1..499
                     /locus_tag="VEP73_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYT26566.1"
                     /translation="VVAGTRVLSARLAEADDITAQALRARPGELVVDLVRIRLADGSP
                     FSLEHARFPARRFPGLLELPLGGSVYELLEEHFGAEPDEAVERIEVVLASPDEAAILD
                     VEPGAPLMAITRTTTDPDGEPIEFSRDLFRADRTRIVVRTESGASRGVPGRARVVELH
                     AQEAV"
     gene            496..>1819
                     /locus_tag="VEP73_08830"
     CDS             496..>1819
                     /locus_tag="VEP73_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HYT26567.1"
                     /translation="MTAPEPVGRIRFMDRQRVNLDGFAVENPELGLTALRSPHDPEPS
                     LVVTGGRVTELDGVPEADFDSIDTYIARHGLDLSVADEAMALTDVAFARLLVDPAVPR
                     HEIIRLSGGATPAKLTRVLALLRPPELVIAMTKLRARRTPSNQAHVTNRLDDPLLLAA
                     DSATAAAFGFREVETTVPVLGDAPSNAVACLIGAAFGADGVLIQCSVEEALELELGMR
                     GLTSYAETVSLYGTEQVFTDGDDTPWSKAFLTSAYASRGMKMRVSSGAGAEVLMAGAE
                     GKSMLYLEARCVALARAIGAQGVQNGGIDGASVAAAVPGGLRELMSENVMAMMRNLES
                     CSGNDALMSESDMRRTSRTLPIALAGSDFVFSGFGSIQRYDNMFGPSNFNAEDIDDFL
                     AMQRDWGVDGGLRTVDPARLGELRREAAQACRAVYAHLGLADFTDEHVE"
BASE COUNT          253 a          665 c          645 g          256 t
ORIGIN      
        1 cgtagtggcc gggaccaggg tcctgtccgc gcggctggcc gaggcggacg acatcaccgc
       61 gcaggcgctg cgcgcccggc ccggcgagct cgtcgtcgac ctggtgcgga tcaggctcgc
      121 ggacggcagc ccgttctccc tcgagcacgc gaggttcccg gcccggcgct ttcccgggct
      181 gctcgaactg ccgctcggag gttcggtgta cgagctgctc gaggagcact tcggcgccga
      241 accggacgag gccgtggagc ggatcgaggt cgtgctcgcc tcgccggacg aggccgccat
      301 cctcgacgtc gagccgggtg cgccgctgat ggcgatcacc aggacgacca ccgaccctga
      361 cggcgaaccg atcgagttct cccgcgacct gttccgggcc gaccgtaccc gcatcgtcgt
      421 ccgcaccgag agcggcgcct cccggggcgt ccccggccgg gcccgcgtcg tcgagctgca
      481 cgcgcaggag gccgtatgac tgctcccgaa cctgtcgggc ggatccggtt catggaccgg
      541 cagcgggtca acctggacgg cttcgccgtc gagaaccccg aactcgggct gaccgcgctg
      601 cgctcgccgc acgaccccga gcccagcctg gtcgtgaccg gtggccgggt gaccgagctg
      661 gacggggtgc cggaggcgga tttcgactcg atcgacacct acatcgcccg gcacggcctg
      721 gacctgtccg tcgcggacga ggcgatggcg ctgaccgacg tggccttcgc ccgcctgctg
      781 gtcgacccgg cggtgccgcg gcacgagatc atccgcctga gcggcggcgc caccccggcc
      841 aagctgaccc gggtactggc cctgctccgg ccgcccgagc tggtcatcgc catgaccaag
      901 ctgcgcgccc ggcgcacgcc gagcaaccag gcccatgtca ccaaccggct cgacgacccg
      961 ctgctgctcg cggccgactc ggccaccgcg gcggcgttcg ggttccgcga ggtcgaaacc
     1021 accgtgccgg tgctcggcga cgcgccgagc aacgcggtgg cctgcctgat cggggcggcg
     1081 ttcggcgctg acggggtgct gatccagtgc tcggtcgagg aggcccttga actcgagcta
     1141 ggcatgcgcg ggctgacctc ctacgccgag accgtctcgc tctacggcac cgagcaggtc
     1201 ttcaccgacg gtgacgacac cccgtggtcc aaggccttcc tcacctcggc ctacgcctcc
     1261 cgcgggatga agatgcgggt gtcgtccggg gccggcgccg aggtgctgat ggccggggcc
     1321 gagggcaagt ccatgctgta cctcgaggcg cgctgcgtcg ccctggcccg cgccatcggc
     1381 gcccagggcg tgcagaacgg cgggatcgac ggggccagcg tggccgcggc ggtgcccggc
     1441 gggctgcgcg agctgatgag cgagaacgtc atggccatga tgcgcaacct agagtcctgc
     1501 tcgggcaacg acgcgctgat gagcgagtcg gacatgcgcc gtacctcccg gacgctgccc
     1561 atcgccctgg ccggctcgga cttcgtattc agcgggttcg ggtccatcca gcgctatgac
     1621 aacatgttcg gcccgagcaa cttcaacgcc gaggacatcg acgacttcct ggccatgcag
     1681 cgggactggg gcgtcgacgg cggactgcgg accgtagatc cggcccggct cggcgaactg
     1741 cgccgcgagg cggcccaggc ctgccgggcc gtctacgctc acctcggcct ggccgacttc
     1801 accgacgagc acgtcgagc
//
LOCUS       DASPYY010000556         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_656551, whole genome shotgun sequence.
ACCESSION   DASPYY010000556 DASPYY010000000
VERSION     DASPYY010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656551"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VEP73_08835"
     CDS             <1..1191
                     /locus_tag="VEP73_08835"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYT26568.1"
                     /translation="DFVFRPDLATLRRIPWQEGTALVMCDLEWEDGSPVVASPRQILR
                     RQLAELAGRGWQALVGTELEFIVFRDSYEEAWRRGYRDLEPANAYNVDYSLLGTARIE
                     PLLRRIRNSMAGAGLTVESAKGECNYGQHEIAFRYAEALATADGHAIYKNGAKEIAAQ
                     DGCSLTFMSKYNEREGNSCHIHLSVRADDEPLLAEGSGEPPGGAPVGREGSGEPQGGA
                     PVNRDPHHGLSELGRHFLAGQLACLRELTLFLAPNVNSYKRFAVGSFAPTAVAWGRDN
                     RTCALRLVGHGPSLRVENRVPGGDVNPYLAISAIVAAGLHGIDKGLELEDEYVGNAYE
                     SDKPRVPTSLEEARELFAGSEVARLAFGAEVVDHYRNMARVELDAFGAAVTDWERYRG
                     FERL"
     gene            1188..2576
                     /locus_tag="VEP73_08840"
     CDS             1188..2576
                     /locus_tag="VEP73_08840"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26569.1"
                     /translation="MTTAGAAPRTGAAFDVRNPATEAVIQRVPAATAEDADAAVDRAA
                     AAFGRWRSVAPSDRGRLLRRLAGRLDTERERLAQLEMRNAGKPIADARGEIGMVVETF
                     AYFAGAPERLLGDTIPVAGGIDVTFREPLGVVGLIVPWNFPLTIASWKLAPALAAGNT
                     VVLKPAELTPLTAVEFGRLAEEAGIPPGVVNVLPGPGRVVGRRLVEHPRVAKIAFTGS
                     TEVGREIMRGAAATVKRVTLELGGKSANVVFADADLDAAAAAAPAAVFGNAGQDCCAR
                     SRILVERSVLDDFLALLEPAVASLRVGDPADEATQMGPLISAPQREAVAAYVPPGAPV
                     AVRGTAPEGPGYWYPPTVLAPVAPGDRAVTEEIFGPVAVVIPFADEAEAVRLANDTIY
                     GLSGSIWTRDGARALRVARAVEAGALSVNSNTSVRVTTPFGGFKQSGVGRELGPHALD
                     HYSEVKNVFIAT"
     gene            2581..>3023
                     /locus_tag="VEP73_08845"
     CDS             2581..>3023
                     /locus_tag="VEP73_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26570.1"
                     /translation="MEGKELIGIGRLHGKVAVITGAASGIGRQSALRFAAEGARVCVV
                     DLADGAGEEVAGEIDGLYVHADVTDQADVERAYEETAARFGGIDVLFNNAGISPPDDA
                     SILDTTLEAWQRVQDVNLKAVFLCCKHGIPHLLRRGGGAVVNTAS"
BASE COUNT          403 a         1140 c         1084 g          396 t
ORIGIN      
        1 gatttcgtct tccggcccga cctcgccacc ctgcgccgca tcccctggca ggaggggacc
       61 gcgctcgtca tgtgcgacct cgagtgggag gacggcagcc cggtcgtcgc ctccccccgc
      121 cagatcctgc gccgccagct cgcggagctc gccggtcgcg gctggcaggc cctggtcggc
      181 accgagctcg agttcatcgt gttccgcgac agctacgagg aggcctggcg caggggctac
      241 cgcgacctcg agcccgccaa cgcctacaac gtcgactact ccctgctcgg caccgcgcgc
      301 atcgagccgc tgctgcggcg catccgcaac tccatggccg gcgccggcct caccgtcgag
      361 tccgccaagg gcgagtgcaa ctacggccag cacgagatcg ccttccgcta cgccgaggcg
      421 ctcgcgaccg ccgacgggca cgccatctac aagaacggcg ccaaggagat cgccgcccag
      481 gacggctgca gcctcacgtt catgtccaag tacaacgagc gcgagggcaa ctcctgccac
      541 atccacctca gcgtgcgcgc cgacgacgag ccgctgctgg cggaggggtc cggggaaccc
      601 ccagggggtg ccccggtggg tcgggagggg tccggggaac cccaaggggg tgccccggtc
      661 aatcgggacc cccaccacgg gctctccgag ctgggacggc acttcctcgc cggccagctc
      721 gcctgcctgc gtgagctcac gctgttcctc gcccccaacg tcaactccta caagcgcttc
      781 gccgtgggca gcttcgcgcc gaccgcggtc gcgtggggcc gcgacaaccg cacctgcgcc
      841 ctgcggctgg tcggccacgg cccctcgctg cgggtcgaga accgcgtgcc cggcggggac
      901 gtcaacccct acctcgcgat cagcgccatc gtcgccgccg gcctgcacgg catcgacaag
      961 ggactcgagc tcgaggacga gtacgtcggc aatgcctacg agtccgacaa gcccagggtg
     1021 cccacgagcc tcgaggaggc acgcgagctg ttcgcgggca gcgaggtggc caggctcgcg
     1081 ttcggcgccg aggtggtcga ccactaccgc aacatggccc gggtcgagct ggacgccttc
     1141 ggtgccgcgg tgaccgactg ggagcgctac cggggcttcg agcgcctgtg acgacggccg
     1201 gcgcggcccc ccggacgggc gccgccttcg acgtcaggaa cccggcgacc gaggcggtga
     1261 tccagcgggt gccggccgcg accgccgagg acgccgacgc cgccgtcgac cgggccgccg
     1321 ccgcgttcgg gcgctggcgc tcggtcgcgc cctccgaccg cgggcggctg ctccgccgcc
     1381 tcgccggccg gctcgacacc gagcgggagc ggctcgccca gctcgagatg cggaacgccg
     1441 gcaagccgat cgccgacgcc cggggcgaga tcggcatggt cgtcgagacc ttcgcgtact
     1501 ttgcgggcgc gcccgagcgg ctcctcggcg acaccatccc ggtcgccggc gggatcgacg
     1561 tgacgttccg cgagccgctc ggggtggtcg ggctgatcgt gccctggaac ttcccgctca
     1621 cgatcgcgtc ctggaagctt gccccggccc tggccgccgg caacaccgtg gtgctgaagc
     1681 cggccgagct cacccccctc accgctgtcg agttcggccg ccttgccgag gaagcgggca
     1741 tcccgcccgg cgtcgtcaac gtgctgcccg gcccggggcg ggtcgtcggc cgccgcctgg
     1801 tcgagcaccc gcgggttgcg aagatcgcgt tcaccggctc gaccgaggtc gggcgcgaga
     1861 tcatgcgcgg cgcggcggcc accgtgaagc gggtcacgct cgagctgggc ggcaagtcgg
     1921 ccaacgtcgt gttcgccgac gccgacctcg acgcggccgc cgcggcggcc ccggccgcgg
     1981 tgttcggcaa cgccgggcag gactgctgcg cgcgctcgcg catcctggtc gagcggtcgg
     2041 tcctcgacga cttcctcgcc ctcctggagc cggccgtggc gtccctgcgg gtgggcgacc
     2101 cggccgacga ggcgacccag atgggcccgc tcatctccgc cccgcagcgg gaggcggtgg
     2161 ccgcctacgt ccccccgggc gccccggtgg cggtgcgcgg caccgccccg gaggggcccg
     2221 gctactggta cccgccgacg gtgctcgcgc cggtggcgcc cggcgaccgg gccgtgaccg
     2281 aggagatctt cggccctgtc gcggtggtca tcccgttcgc cgacgaggcc gaggcggtgc
     2341 ggctcgccaa cgacacgatc tacggcctct ccgggtcgat ctggaccagg gacggcgccc
     2401 gtgccctgcg tgtcgcccgg gccgtcgagg cgggcgcgct ctcggtcaac tccaatacct
     2461 cggtccgggt cacgacgccg ttcggcgggt tcaagcagtc gggcgtcggg cgcgagctcg
     2521 gcccgcacgc cctcgaccat tacagcgagg tgaagaacgt cttcatcgcg acctgacgcc
     2581 ttggaaggga aggagctgat cgggatcgga cggctgcacg gcaaggtcgc cgtcatcacc
     2641 ggtgcggcca gcggcatcgg gcgccagagc gcgctccgct tcgcggccga gggcgccagg
     2701 gtctgcgtcg tcgacctcgc cgacggggcg ggcgaggagg tggccggcga gatcgacggg
     2761 ctctacgtcc acgccgacgt gaccgaccag gccgacgtgg agcgcgccta cgaggagacg
     2821 gcggcgcggt tcggcggcat cgacgtcctg ttcaacaacg ccggcatctc cccgcccgac
     2881 gacgcgtcga tcctggacac gaccctcgag gcgtggcagc gggtgcagga cgtgaacctc
     2941 aaggcggtgt tcctctgctg caagcacggc atcccgcacc tgctccggcg cggcggcggc
     3001 gctgtggtca acaccgcgtc gtt
//
LOCUS       DASPYY010000557         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1077307, whole genome shotgun sequence.
ACCESSION   DASPYY010000557 DASPYY010000000
VERSION     DASPYY010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077307"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VEP73_08850"
     CDS             <1..855
                     /locus_tag="VEP73_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26571.1"
                     /translation="PRERPRPGRPDRVSRRRTPGLRAMLAVALLVAAGDGFLLWGRTL
                     ATPVGAAAARARFLPGPAAPGPGPRPPAGLYRYRTTGRETVDRFGVEHAYPAETVRVV
                     TDLGGCRWRETVPLFDQHTETYDFCADSGLRDARDVAYATSLTYFLVPGVQRFSCAVP
                     GRRLMTGAAPGTVVRWRCAEGASVSDNTTTYLGTATHLGTASLGGGTLRDGPGLAGAR
                     HLRVVTALTGQSTGAAVRDLWLDRDGLVLREERLVRLVVRSGFVGRVVYDERATFTLV
                     AREPVPGR"
     gene            991..2103
                     /locus_tag="VEP73_08855"
     CDS             991..2103
                     /locus_tag="VEP73_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT26572.1"
                     /translation="MLAMAIKEFRQLRRDRRTLAMLVVLPLVLLTVFGYAASFDVPSV
                     RTIVVGPRAEAVAARVPAQLDVVAVRPADGRAEAVRELRDGRAGVALVTGGGDAAGGA
                     PVTALVDGADLFSARAAVQALTQRPGAGQAAGGPRVVPEVLFNPELRTPVIMVPGLVG
                     IILVFVGTMATALGVVRERQSGTLEQLAVMPFRPRDVFLGKVLPYFGVATVDLTVILV
                     AAILLFHVPFRGSVAVFALGALLFLFVTLGLGVLISTVSENQGQAIQLAIMTLLPQVL
                     LSGLIFPLHAMAAGVRWIGYVLPLTYFIQIARGVMVRGTPLEALAVPLLALAGLGLAV
                     FTLSVVRFRRDLAPAGRPGEREPRPEREPATVGGAP"
BASE COUNT          217 a          823 c          806 g          316 t
ORIGIN      
        1 ccgcgtgagc gtccccgccc cgggcgcccg gaccgggtga gcaggcggcg cacgcccggg
       61 ctgcgcgcca tgctcgccgt cgccctgctc gtcgcggcgg gcgacggctt cctgctctgg
      121 gggcgcaccc tcgcgacccc ggtcggcgct gccgcggcca gggcccgctt cctgcccggc
      181 ccggccgcgc ccgggcccgg cccccggccg ccggccgggc tctaccggta ccggacgacg
      241 ggccgcgaga ccgtggacag gttcggcgtc gagcacgcct accccgccga gacggtccgg
      301 gtggtcaccg acctcggcgg ctgccgctgg cgggagaccg tccccctgtt cgaccagcac
      361 accgagacct acgacttctg cgccgactcg ggactgcgcg acgcgcgcga cgtcgcgtac
      421 gcgaccagcc tcacgtactt cctcgtcccg ggggtccagc ggttctcgtg cgccgtcccg
      481 ggccgccgcc tcatgaccgg ggccgcaccc gggaccgtgg tgcgctggcg ctgcgccgag
      541 ggcgcgtcag tgtccgacaa caccaccacg tatctcggca cggctacgca tctcggcacg
      601 gcgtccctcg gcggcgggac gctccgggac gggccgggtc tggcaggcgc gcgccacctg
      661 cgggtcgtca ccgcgctgac cggccagtcg accggtgccg ccgtccgcga cctgtggctc
      721 gaccgtgacg ggctggtcct gcgggaggag cgcctggtcc gcctggtcgt ccggtccggc
      781 ttcgtcgggc gggtcgtcta cgacgagcga gccaccttca cgctcgtcgc ccgggagcct
      841 gtcccgggcc ggtgagccaa cctgagcggt gagcccgccc gggggtgcga ggccgaccgg
      901 ggccgccagc ttgccccttg ccaaagatct caacaagcgt agaattcaac gcgcagagag
      961 ttcatcgtct ggagacttct gggaggcgtc atgctggcga tggcgatcaa ggagttccgg
     1021 cagctccgcc gggaccggcg caccctggcg atgctcgtcg tgctgccgct cgtgctgctg
     1081 accgtgttcg gctacgcggc cagcttcgac gtgccctcgg tcaggacgat cgtggtcggg
     1141 ccgcgggccg aggcggtcgc cgcgcgggtc cccgcccagc tcgacgtggt cgcggtgcgg
     1201 cccgcggacg ggcgggccga ggccgtccgc gagctgcgcg acgggcgggc gggggtggcc
     1261 ctggtcaccg gcggcgggga cgccgccggc ggcgcgccgg tgaccgcgct cgtcgacggc
     1321 gccgacctgt tctcggcccg ggcggcggtc caggcgctca cccagcggcc aggggcgggg
     1381 caggcggcgg gcggcccccg ggtggtgccc gaggtcctgt tcaaccccga gctgcgcacg
     1441 ccggtcatca tggtgcccgg cctggtcggc atcatcctcg tgttcgtggg gacgatggcg
     1501 accgcgctcg gcgtcgtccg cgagcgccag agcggcaccc tcgagcagct cgcggtcatg
     1561 ccgttccggc cgagggacgt gttcctcggc aaggtgctgc cctacttcgg cgtcgccacc
     1621 gtcgacctca cggtgatcct cgtggccgcg atcctgctgt tccacgtgcc gttccggggg
     1681 tcggtggccg tcttcgcgct cggcgccctg ctcttcttgt tcgtcaccct cgggctcggc
     1741 gtgctgatct cgaccgtctc ggagaaccag gggcaggcga tccagctcgc gatcatgacc
     1801 ctgctccccc aggtgctcct gtccggcctc atcttcccgc tgcacgcgat ggcggccggc
     1861 gttcgctgga tcgggtacgt cctcccgctc acctacttca tccagatcgc ccggggggtg
     1921 atggtccggg ggacgcccct cgaggcgctc gccgtgcccc tgctcgcgct ggccggtctg
     1981 ggccttgccg tgttcacgct ctcggtcgtc cgcttccggc gcgacctggc cccggcaggg
     2041 cgacccggtg agcgcgagcc caggcccgag cgcgagcccg cgacggtggg aggtgcgccg
     2101 tgaggaggac ggacgcgccc ctggcgggcc gcgggcgggg cacgcccgcc ccggcggcgg
     2161 gc
//
LOCUS       DASPYY010000558         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_517299, whole genome shotgun sequence.
ACCESSION   DASPYY010000558 DASPYY010000000
VERSION     DASPYY010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517299"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VEP73_08860"
     CDS             <1..300
                     /locus_tag="VEP73_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HYT26573.1"
                     /translation="PAKPRPTATTPVPSKGVSIVLRYSSPSWTLVKIDGKVAFQGTPA
                     ASEQRPFNADRAIDVILGNAGGVQLTVNGKLLGAAGAPGAVWRGSFRPGDPGAHG"
     gene            complement(400..633)
                     /locus_tag="VEP73_08865"
     CDS             complement(400..633)
                     /locus_tag="VEP73_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26574.1"
                     /translation="MTAWNEISWDEAMRRAQTALLASEELYDEIDAQEGRSNVDIEKH
                     LRRAEVKASHARSWVAIAQALGTQHDRYGSAGG"
     gene            903..>2176
                     /gene="rimO"
                     /locus_tag="VEP73_08870"
     CDS             903..>2176
                     /gene="rimO"
                     /locus_tag="VEP73_08870"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYT26575.1"
                     /translation="MDPAEPRKVAIVTLGCGRNEVDSDQAAGLLAASGFEMVDDPEQA
                     DAIVVNTCAFIEAAKRESIDTVMDAAGLKAQGRARTVLVMGCLAERYTDTLRAELPEA
                     DAIVPFADYVRLPDVLRSTLGTVAPASPGPSRPALAPPAGRRALPMVFPTPAGTPFPA
                     RGAVRGPVALVKLAEGCDRDCSFCAIPSFRGGFRSRRPTEVLDEVAWLAGQGVSEVVL
                     VAENSTSYGKDLGGRSALVHLLRDLAAVDGLRRIRLQYLQPDELTPGLLEEMAANPVV
                     CSYFDLSLQHASAPVLRRMRRGGSREAFLGLVGRIRALDPDAAFRSNFILGFPGERRQ
                     DVRELEDFLEEARLDWVAFFAYSPEDGTAALGLDRQVPARTARARVERLTELQARVLD
                     EVQAAWVGRRLEVLVDRVDRDGTAEGRSFREAP"
BASE COUNT          260 a          823 c          781 g          312 t
ORIGIN      
        1 cccgccaagc ccaggccgac cgccacgacg cctgtgccct ccaagggcgt cagcatcgtg
       61 ctgcgctact cgtcgccctc ctggaccctg gtgaagatcg acggcaaggt cgccttccag
      121 ggcacgccgg ccgcgtcgga gcagcgcccg ttcaacgccg acagggcgat cgacgtgatc
      181 ctcggcaacg ccggcggggt ccagctcacc gtgaacggca agctgctcgg tgcggccggc
      241 gcgccaggcg cggtctggcg gggcagcttc aggccgggcg acccgggcgc gcacggctag
      301 cgcacctggc cacccggggc gcgccgcgag cgctggtcga cacggtggtc aaggggcacg
      361 agcatcacag cggcctggcc atcggccagc aggtccacgc tacccgccgg cggatccgta
      421 ccggtcgtgt tgggtgccaa gggcctgggc gatcgcgacc caggaccggg catgcgaggc
      481 cttcacctcg gccctgcgca gatgcttctc gatatcgacg ttcgagcgac cctcctgcgc
      541 gtcgatctcg tcgtacaact cctcggacgc gagcagcgcc gtctgggccc gccgcatcgc
      601 ctcgtcccag ctgatctcgt tccacgcggt catcgcgctg ctcctcccgg tcggcgacca
      661 tgccctgctg agggtcgcgg ccctccgtca tccggcacga gtatgcgctg gagcggtgtt
      721 gcgtccccct ggcgagtcgg tggcgcggcg cttgccgccg ttcgagcggg tagtctcttg
      781 tagctcccgg ccgggggccg agccgggatc gtcctgccgc tcccgtacag ggccggagca
      841 ccgggccgcc cgaggtcggt gggtcaccgt gacctccagg atgttgagga gccatgacga
      901 cgttggatcc tgccgagccg cggaaggtcg ccatcgtcac gctcgggtgc gggcgtaacg
      961 aggttgactc cgaccaggcc gccggcctgc tggcggcgtc ggggttcgag atggtcgacg
     1021 accccgagca ggcggacgcg atcgtcgtca acacctgcgc cttcatcgag gcggccaagc
     1081 gggagtcgat cgacaccgtg atggatgcgg ccgggctgaa ggcgcagggc cgggccagga
     1141 ccgtcctggt gatgggctgc ctggccgagc gctacaccga caccctgcgc gccgagctgc
     1201 cggaggcgga cgcgatcgtc ccgttcgccg actacgtgcg cctcccggac gtgctcaggt
     1261 cgacgctcgg gaccgtcgcg ccggcctcac cgggcccgtc ccggccggcg ctcgccccac
     1321 cggccgggcg gcgggcgctg ccgatggtgt tccccacccc ggcaggcacg cccttcccgg
     1381 cccggggcgc cgtccgcggt ccggtggccc tcgtcaagct ggccgagggc tgcgaccggg
     1441 actgctcgtt ctgcgcgatc ccgtcgttcc ggggcggctt ccgctcccgc cggccgaccg
     1501 aggtcctcga cgaggtcgca tggctggccg gccagggcgt gtccgaggtc gtgctggtcg
     1561 ccgagaactc cacctcctac ggcaaggacc tgggcggccg ctcggccctc gtccacctcc
     1621 ttcgcgacct cgccgccgtg gacgggctgc gccggatccg cctgcagtac ctgcagcccg
     1681 acgagctcac ccccggcctg ctcgaggaga tggccgccaa cccggtcgtc tgctcgtact
     1741 tcgacctgtc cctgcagcac gcgagcgcgc ccgtgctgcg ccgcatgcgc cggggcgggt
     1801 ccagggaggc gttcctgggc ctcgtcggcc gcatccgcgc cctcgacccg gacgccgcct
     1861 tccgctccaa cttcatcctg gggttcccgg gcgagcggag gcaggacgtc cgcgagctcg
     1921 aggacttcct cgaggaggcg cgcctggact gggtcgcctt cttcgcctac tcgcccgagg
     1981 acggcaccgc cgcgctgggg ctcgaccgcc aggtgccggc ccgcaccgcc cgggcccggg
     2041 tcgagcgcct caccgagctg caggcgcgcg tgctcgacga ggtccaggcg gcctgggtcg
     2101 gccgccggct cgaggtgctg gtcgaccggg tcgaccgcga cggcacggcg gagggccgca
     2161 gcttccgcga ggcgcc
//
LOCUS       DASPYY010000559         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1077339, whole genome shotgun sequence.
ACCESSION   DASPYY010000559 DASPYY010000000
VERSION     DASPYY010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077339"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..988)
                     /locus_tag="VEP73_08875"
     CDS             complement(77..988)
                     /locus_tag="VEP73_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYT26576.1"
                     /translation="MRRPLAVLVTLALAGAAVAVGVSTLFLRGNAAPAVTVQRAHKSA
                     AGPDFKGRITFLVVGSDSGAPKFGRGGVAERGRADSLHLVVLDRGRRQGIVMGFPRDS
                     YVPIPGHGTNKINAAMSLGGPQLLIRTFEQLTRIRIDYLALTSFDGLTDLVNRVGGVQ
                     VDVDMNIRDHFAGAFLNKGRQRLSGTAALAYSRARKTVPGGDIARSRHQAQILLGGLA
                     TYQAQVLKDPTRVMKWLAIMNDEVKTDLPFPEQLRLALFARAVSPARLNNVVVPNQPG
                     TAGSASIVRLLPGAYSLFARVRAGQLR"
     gene            complement(985..1731)
                     /locus_tag="VEP73_08880"
     CDS             complement(985..1731)
                     /locus_tag="VEP73_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26577.1"
                     /translation="MPVRTRLGTLRRAFRLEIRPEIAEISSPTLEISTSPGTRSQPRS
                     GALSRSTRVAAALAVALLLASCTSGKRSSAPAPGQPSTSGAAAGTTTPAAPLRLDLVA
                     DAQGPERGFDPKVAARRATPAARQFLTRYVGVAFLDPGQRRAGWRDLLALFDGPLQPA
                     ARRDLDSLSLGTAAAQVKRVQPRLAKASVTFLYRGGRPVGATARLAFDGAADTEQGTG
                     PVRLRSIFQLLWTAKGWRIAAYQSQTGASA"
     gene            1915..2259
                     /locus_tag="VEP73_08885"
     CDS             1915..2259
                     /locus_tag="VEP73_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYT26578.1"
                     /translation="MDLDLNVRKEGDHAVLEVGGEVDVYTAPKLREKLIELVSDGEYN
                     VVVDLEKVDFLDSTGLGVLVGGLKRVRNHDGSLQLVCTQEKILKIFRITGLTKVFPIH
                     ASVAEAISSSGG"
     gene            2269..2709
                     /locus_tag="VEP73_08890"
     CDS             2269..2709
                     /locus_tag="VEP73_08890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26579.1"
                     /translation="MDPSEPVPRVVRMAFPPEPALLGTVRLVVGIVARKAGVDEEGVE
                     DLKVAVSETCVVAVGDLAWAGLSTPIELDLIEEPDRFGVEVRDRAPAPGHAPSSNGEV
                     DDRQFGLALVGALVDDLHSAALEGGGHRTRFWMRRGGAGLPPFA"
     gene            3046..>3952
                     /locus_tag="VEP73_08895"
     CDS             3046..>3952
                     /locus_tag="VEP73_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HYT26580.1"
                     /translation="MLVSVLALVVAFVLVRQVLAADQGSREMREIAAAIQEGSRAYLN
                     RQFRTLAVFVALLFFLLLLLDADDNSVRIGRSVAFLVGAAFSAVAGRTGMGLAVRANV
                     RVAAAAERGGLLKAMPIAFRAGGVAGMFVVGLGLLGASAVVLLYKGQAANVLFGFGFG
                     AALLAMFMRVGGGIFTKAADVGADLVGKVEQSIPEDDPRNAATIADNVGDNVGDCAGM
                     AADLFESFEVTLVAAIVLGTAAFPGRPIVGLLFPLMVQAVGIVASIAGIWLVRPRSEG
                     ESGMTVINRGFFAAAVVSAVLVGVVS"
BASE COUNT          502 a         1320 c         1564 g          566 t
ORIGIN      
        1 gtcggagtcg gacaccccgc catggtagcc gcggcggggg acagggccgc ccgccgcgcc
       61 gccggcggcg cgggcgtcag cggagctgtc cggcccgcac gcgggcgaag agggagtagg
      121 cgcccggcag gagccggacg atcgaggcgc tgcctgcggt accgggctgg ttggggacga
      181 cgacgttgtt cagccgggcc ggggagacgg cgcgggcgaa caaggcgagg cggagctgct
      241 ctgggaacgg cagatcggtc ttgacctcgt cgttcatgat cgcgagccac ttcatgaccc
      301 gggtcgggtc cttgaggacc tgggcctggt aggtggcgag ccccccgagc aggatctggg
      361 cctggtggcg ggagcgggcg atgtcgccac caggcacggt cttgcgggcg cgggagtagg
      421 cgagcgcggc cgtgcctgac agccgctggc gtcccttgtt gaggaacgct ccggcgaagt
      481 ggtcgcggat gttcatgtcg acgtcgacct ggaccccgcc gacccggttg acgaggtcgg
      541 tgaggccgtc gaaggaggtg agcgcgaggt agtcgatcct gatgcgggtg agctgctcga
      601 aggtgcggat gaggagctgc gggccgccga gggacatggc cgcgttgatc ttgttcgtgc
      661 cgtgcccggg gatcgggacg tagctgtcgc gcgggaagcc catgacgatg ccctggcgcc
      721 tgccgcggtc gagcacgacg aggtgcaggg agtcggcacg cccccgctcg gcgacgccgc
      781 cgcggccgaa cttgggcgcc ccggagtccg agccgaccac gaggaaggtg atccggccct
      841 tgaagtcggg gcccgccgcg ctcttgtggg cacgctggac ggtcacggcc ggcgcggcgt
      901 tgccgcgcag gaagagggtg gacacgccga ccgcgacggc cgccccggcc agcgccaggg
      961 tcacgaggac ggcaaggggg cgtctcatgc ggaggctccc gtctggctct ggtaggcggc
     1021 gatccgccag cccttggcgg tccacaggag ctggaagatc gagcgcaggc ggaccgggcc
     1081 ggtgccctgc tcggtgtcgg cggcaccgtc gaaggcgagc cgcgcggtgg cgccgaccgg
     1141 ccgcccgccc cggtagagga acgtgaccga ggccttggcg aggcggggct ggacgcgttt
     1201 cacctgggcg gcggcggtcc ccagggagag ggagtcgagg tcgcgcctgg cggccggctg
     1261 caagggaccg tcgaagaggg cgagcaggtc gcgccagccg gcccgccgct gcccggggtc
     1321 gaggaacgcg acgccgacgt agcgggtgag gaactgccgg gcggcggggg tcgcccgccg
     1381 ggcggcgacc ttggggtcga agccgcgctc cggcccttgc gcgtcggcca cgaggtcgag
     1441 ccgcagcggg gcggccggcg tggtggtgcc cgcggccgca cccgaggtgg acggctggcc
     1501 gggcgcgggt gccgagctcc tcttgccgga ggtgcaggag gccagcagca gggcgaccgc
     1561 gagcgcggcg gcgacacggg tggacctgga cagggctccg ctccttggct gcgatctggt
     1621 ccccggggag gttgagatct cgagcgtggg acttgagatc tcagcgatct cgggccggat
     1681 ctcgagccgg aaggcgcggc gtagggtacc gagccgcgtc cgaacgggca acccgcgccg
     1741 ccgccccacg ggcaacgcgg ggcgacgcgc gccgccgccg gtccgcgtcc gcgcttgccg
     1801 ctggtgggcg gcgagcctat ggtgacgctt tcttgccccg tcggggggcc ggcgctagcc
     1861 ttccggctgc gttggcaacg gcgaccggag accgaccgaa gacgaggaga gggcatggac
     1921 ctcgacctga acgtgcggaa ggaaggggac cacgcagtcc tcgaggttgg gggcgaggtc
     1981 gacgtctaca ccgccccgaa gcttcgcgag aagctgatcg agctcgtcag cgacggggag
     2041 tacaacgtcg tcgtcgacct cgagaaggtg gacttcctgg actcgaccgg cctcggcgtg
     2101 ctggtcggcg ggctcaagcg ggtccgcaac cacgacggct cgctgcagct ggtctgcacc
     2161 caggagaaga tcctgaagat cttccgcatc accggcctga ccaaggtgtt cccgatccac
     2221 gcctcggtcg ccgaggcgat ctcgagctcg ggcgggtagc acgggcccgt ggacccgagc
     2281 gagcccgtcc cgagggtcgt ccgcatggcg ttcccgcccg agccggcgtt gctcggcacg
     2341 gtccgcctgg tcgtggggat cgtggcgcgc aaggccggcg tggacgagga gggcgtcgag
     2401 gacctcaagg tggcggtgtc ggagacctgc gtggtggccg tgggcgacct ggcctgggcc
     2461 ggcctgtcca cgccgatcga gctggacctg atcgaggagc ccgaccggtt cggggtcgag
     2521 gtgcgcgacc gcgccccggc ccccgggcat gccccgtcgt cgaacggcga ggtcgacgac
     2581 cgccagttcg gcctcgcgct ggtcggcgcc ctggtcgacg acctgcacag cgccgcgctg
     2641 gaaggcggcg ggcaccgcac ccggttctgg atgcgccgcg gcggtgccgg gctgccgccg
     2701 ttcgcctgag gcgggcggta cggagcagcc gggatggccg accggatggt ccggaggagt
     2761 tgcccggccc gcacgtcgat ggctagtatt cccccattct cgcgggtcct ggacacggcg
     2821 ggcgcgcacc tcggcacggg gtgtggcggt gctgcggtca gcccgcgatt cgcgctttcc
     2881 cgctcccgcc cgccagatgc ctccggctcg tgcggggtcg agacaacgtc ccaacgacgg
     2941 ggcgtctgag gaggtctgat gggccacgta cggctgctcg cgcaggaggg cagcgtccga
     3001 gggatccagt tccgcgggtt cgagaacacg acgctgctgt tggtcatgct ggtctccgtc
     3061 ctcgccctcg tcgtcgcgtt cgtgctggtc aggcaggtcc tcgccgcgga ccagggcagc
     3121 agggagatgc gcgagatcgc cgcggccatc caggagggca gccgcgccta cctgaaccgc
     3181 cagttccgca ccctggcggt gttcgtcgcc ctgctgttct tcctgctgct gctgctcgac
     3241 gccgacgaca actcggtgcg catcggccgc tcggtcgcct tcctggtcgg ggccgccttc
     3301 tccgccgtcg cgggccggac cgggatgggt ctggccgtgc gcgccaacgt ccgggtggcc
     3361 gccgcggccg agcggggcgg cctgctcaag gccatgccga tcgcgttcag ggccggcggg
     3421 gtggcgggca tgttcgtcgt cggcctcggg ctgctcgggg cctcggccgt ggtcctgctc
     3481 tacaagggcc aggccgccaa cgtgctgttc ggcttcggct tcggcgccgc cctgctcgcg
     3541 atgttcatgc gggtcggcgg tgggatcttc accaaggcgg ccgacgtggg cgcggacctc
     3601 gtcggcaagg tggagcagtc gatccccgag gacgaccccc gcaacgctgc caccatcgcc
     3661 gacaacgtcg gcgacaacgt gggcgactgc gccgggatgg ccgccgacct gttcgagtcc
     3721 ttcgaggtca cgctggtcgc cgccatcgtg ctcggcaccg ccgcgttccc cggccggccg
     3781 atcgtcgggc tcctgttccc cctcatggtg caggccgtcg ggatcgtggc ctcgatcgcc
     3841 gggatctggc tggtccgccc ccgctccgag ggcgagagcg ggatgacggt catcaaccgg
     3901 gggttcttcg ccgccgcggt ggtctcggcc gtgctcgtcg gcgtcgtgtc gg
//
LOCUS       DASPYY010000560         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1921854, whole genome shotgun sequence.
ACCESSION   DASPYY010000560 DASPYY010000000
VERSION     DASPYY010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921854"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VEP73_08900"
     CDS             <1..873
                     /locus_tag="VEP73_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYT26581.1"
                     /translation="IGTGGAVGHVMEYRGSTIRALSMEGRMTVCNMSIEGGARAGMVA
                     PDDTTFQYVAGRPYAPKGADWDRAVADWRTLPTDEGATYDRSVTIDAGSLEPMVTYGT
                     NPGMGLPVTARVPDPDHAADPARRQALRKALAYMDLRPGEPLLGHPVDVVFIGSCTNS
                     RISDLRQAAAVLQGRKVDPRVRVLVVPGSQDVKRQAEAEGLARVFRSAGAEWREAGCS
                     MCIAMNGDQLAPGQYAVSTSNRNFEGRQGAGGRTFLASPLTAAASALAGSIADARRLG
                     ETATVTGPRSGEGV"
     gene            876..1496
                     /gene="leuD"
                     /locus_tag="VEP73_08905"
     CDS             876..1496
                     /gene="leuD"
                     /locus_tag="VEP73_08905"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYT26582.1"
                     /translation="MEPLRAFTSAVVPLLADDVDTDQIIPARFLKTTDKTGLGDNLFA
                     DWRYDADGVPRPDFVLNRPGMAGRRVLLAGDNFGAGSSREHAPWALTAFGFRAVLATS
                     FADIFRANALKNGLLPVQLDPVAHARVAALVQADPDAEVTVDLQAGAVTLPDGTAARF
                     EIDPFSRSMLLEGTDELGYLLARADAVAAYEAAHPSRVDTTLPVVG"
     gene            1519..2886
                     /gene="aroA"
                     /locus_tag="VEP73_08910"
     CDS             1519..2886
                     /gene="aroA"
                     /locus_tag="VEP73_08910"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYT26583.1"
                     /translation="MTPVPFGTPGRAPDPPVPSGTPGRAPDRRAAVDAAAVELVPAGP
                     VRARLAAPASKSVTNRALACAALADGDTVLAGPLDSDDAAAMREAAGALGAPVADADG
                     AWLVRGTGGRPRSPAGPLDARLSGTTMRFLTALATLTPTGATVSGRPGLLTRPTGPLT
                     RALAALGARVADSDGHPPVTAAGGGLAGGTVTVAAAGSSQFASAVLLVAPYARAPVRL
                     TADGLGAPGYVELTAEVMRWFGAAAERVGEATWLVRPCRYQGRTLEVEHDASAAAHLY
                     ALAAATGGEVTVTNAAPGSRQPDARLPELLARMGAAVRRAGTALTVAGPDVLAPVDAD
                     LRAMPDQVTTVAALAALAPGTSRLTGVAVVRGHETDRLAALATELAKLGVGVEERPDG
                     LVVHGGRAAGPARIATHGDHRLAMAFAAVAARVPGVAIADPGCVDKTYPGFWRDLAAA
                     GLHPR"
BASE COUNT          328 a         1228 c         1124 g          349 t
ORIGIN      
        1 atcgggacgg gcggcgccgt gggccacgtc atggagtacc ggggcagcac gatccgtgcc
       61 ctgtcgatgg agggtcggat gacggtgtgc aacatgtcga tcgagggcgg cgcccgggcc
      121 ggcatggtcg cccccgacga cacgacgttc cagtacgtcg ccgggcgccc ctacgcgccg
      181 aagggcgccg actgggaccg ggcggtcgcc gactggcgca ccctgccgac cgacgagggc
      241 gccacctacg accggtcggt cacgatcgac gccgggtcgc ttgagccgat ggtcacctac
      301 ggcaccaacc cgggcatggg gctgccggtc accgcccggg tgccagaccc cgaccacgcg
      361 gccgaccccg cccgccgcca ggcgctgcgc aaggcgctcg cctacatgga cctgcggccg
      421 ggcgagccgc tgctcggcca cccggtcgac gtcgtgttca tcggctcgtg caccaactcg
      481 cgcatctccg acctgcgcca ggccgccgcc gtcctccagg gccgcaaggt cgacccgcgg
      541 gtgcgggtcc tcgtcgtgcc cgggtcgcag gacgtcaagc gccaggccga ggccgagggg
      601 ctcgcccggg tgttccggtc cgccggggcg gagtggcggg aggccggctg ctcgatgtgc
      661 atcgcgatga acggcgacca gctcgcgccg gggcagtacg ccgtgtcgac ctccaaccgc
      721 aacttcgagg ggcgccaggg cgccggcggg cggacgttcc tcgcgagccc gctgaccgcc
      781 gccgcgtccg cgctcgccgg ctcgatcgcc gacgctcgcc ggctcggcga gacggcgacc
      841 gtgaccggcc cgcggtccgg ggagggggtg tgacggtgga gccgctgcgc gccttcacga
      901 gcgccgtcgt gccgctgctc gccgacgacg tcgacaccga ccagatcatc ccggcccgct
      961 tcctcaagac gaccgacaag accgggctcg gcgacaacct gttcgccgac tggcgctacg
     1021 acgcggacgg cgtcccccgg ccggacttcg tgctgaaccg gcccgggatg gccggacggc
     1081 gcgtgctgct cgccggcgac aacttcgggg ccggctcctc gcgggagcac gcgccctggg
     1141 cgctcaccgc cttcggcttc cgtgccgtgc tcgccacctc cttcgccgac atcttccggg
     1201 ccaacgcgct gaagaacggg ctgctgcccg tccagctcga cccggtcgcg cacgcccgcg
     1261 tcgccgcgct cgtccaggcg gacccggacg ccgaggtgac cgtcgacctc caggccggag
     1321 cggtgacgct ccccgacggg accgcggccc gcttcgagat cgacccgttc tcgcgctcca
     1381 tgctcctcga gggcaccgac gagctcggct acctgctcgc ccgggccgac gcggtcgccg
     1441 cgtacgaggc cgcccacccg tcccgcgtcg acacgactct cccggtggtg gggtgagccc
     1501 gtcggcaccg ccggggcggt gacccctgtc ccgttcggca ccccggggcg tgcccccgat
     1561 ccccctgtcc cgtccggcac cccggggcgt gcccccgacc ggcgcgccgc cgtcgacgcc
     1621 gcggcggtcg agctggtgcc ggccggaccg gtgcgggcca ggctggccgc gcccgccagc
     1681 aagagcgtga cgaaccgggc gctcgcttgc gccgcgctcg ccgacggcga caccgtgctc
     1741 gcgggcccgc tcgacagcga cgacgccgcg gcgatgcggg aggcggccgg ggcgctgggc
     1801 gcgccggtcg ccgacgcgga cggcgcgtgg ctcgtgcggg gcaccggcgg ccggccgcgc
     1861 tccccggccg gcccgctgga cgcgaggctg tccgggacga ccatgcggtt cctcaccgcg
     1921 ctcgcgaccc tgaccccgac cggagcgacc gtctcgggcc gtcccggcct gctcacccgc
     1981 ccgacgggcc cgctcacgcg cgcgctcgcg gccctcgggg cgcgggtcgc ggactccgac
     2041 gggcacccgc cggtgaccgc cgcgggcggc gggctcgcgg gcgggacggt gacggtcgcc
     2101 gcggcagggt cgagccagtt cgcgagcgcc gtgctcctcg tcgcgcccta cgcccgcgcc
     2161 ccggtccggc tcaccgccga cgggctcgga gcgcccggct acgtggagct gaccgccgag
     2221 gtcatgcgct ggttcggcgc cgcggccgag cgggtcggcg aggcgacctg gctcgtccga
     2281 ccctgccgct accagggtcg cacgctcgag gtcgagcacg acgcgagcgc cgccgcgcac
     2341 ctgtacgccc tggccgccgc gaccggtggc gaggtgaccg tcacgaacgc ggcccctggc
     2401 tcgcgccagc ccgacgcgcg cctcccggag ctgctcgcac gcatgggcgc ggcggtccgc
     2461 cgcgccggga ccgcgctcac cgtcgccggg ccggacgtcc tcgccccggt cgacgcggac
     2521 cttcgggcaa tgcccgacca ggtcaccacc gtcgccgcgc tcgccgcgct cgcgccgggc
     2581 acgtcccgcc tgaccggcgt cgcggtcgtc cgcggccacg agaccgaccg gctcgccgcg
     2641 ctcgccaccg agctggccaa gctcggcgtc ggggtggagg agcggcccga cgggctggtg
     2701 gtgcacgggg ggcgggccgc cgggccggcg cgcatcgcga cccacggtga ccaccgcctg
     2761 gcgatggcct tcgccgcggt cgccgcgcgc gtccccggcg tcgccatcgc cgaccccggc
     2821 tgcgtcgaca agacctaccc gggcttctgg cgcgacctcg ccgccgccgg gctccacccc
     2881 cgctgaggcc cgggcgccgc ctgccaggca ccggccggct cgtgtggggc ggtcgccctg
     2941 cccctcggcg tgaccgaccg cggctcggtg gaggcgttcg ccgacgcggt cgccgaccgc
     3001 ttcgggcgcg cggacgtgct ggtcaacaa
//
LOCUS       DASPYY010000561         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_517477, whole genome shotgun sequence.
ACCESSION   DASPYY010000561 DASPYY010000000
VERSION     DASPYY010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517477"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VEP73_08915"
     CDS             <1..825
                     /locus_tag="VEP73_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT26584.1"
                     /translation="LRDPKAPKGSSVTVALGIDVPAQRGRADSGDLEVDLTELFTEAA
                     SVAADLGVGIALFVDEMQDVPSADVSALCAACHELSQTGGPLIVVGAGLPHLPSVLSA
                     SKSYSERLFRYARIDRLDREAADLALLAPAQREDVTFTSDALDALYAAADGYPYFVQA
                     YGKVTWDVAASSPVTAADVSVAAPEAEGELAVGFFGSRYERATPAEREYMRAMASTGD
                     DPVPTSVVAEELARKPSSLSPARDGLIKKGLIYSSERGLIAFTVPHFGKFLRSQPV"
     gene            complement(914..>1533)
                     /locus_tag="VEP73_08920"
     CDS             complement(914..>1533)
                     /locus_tag="VEP73_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methylamine utilization protein MauD"
                     /protein_id="HYT26585.1"
                     /translation="LPATIVRTGTALFFLVAVGALNEMRQRRPDSGCGCFGELSGTPI
                     GLRTIARPGLLCASAVATLGLPPLRKPSSPAEAELWLAVLAFELTLIAFLSPELGEIM
                     VRLGYSEPCELRRLPVGRTMTMLHASSQWRRYAGQISAAAPSDVWREGCWRFVVYPGS
                     ARGRPVDIVFAVYLQSRRPAIRAAVLDADTDEVLGPTGTRESAVI"
BASE COUNT          224 a          550 c          553 g          206 t
ORIGIN      
        1 ctgcgcgatc ccaaggcgcc caagggatcc tcggtcaccg tcgcgctcgg catcgacgtg
       61 cccgctcagc gcggccgggc ggactccggc gatctcgagg tcgacctgac ggaactgttc
      121 accgaggccg cgtcggtcgc cgccgacctc ggtgtcggca tcgcgctgtt cgtcgacgag
      181 atgcaggacg tgccctcggc cgacgtgtcc gccctctgcg ccgcctgcca tgaactctcg
      241 cagaccggcg gcccgctcat cgtggtcggg gcgggcctgc cgcacctgcc gtcggtgctc
      301 tcggcgagca agagctactc cgagcggctg ttcaggtacg cccggatcga ccgcctggac
      361 cgcgaggccg ccgacctggc gctgctcgcc ccggcccagc gcgaggacgt gaccttcacc
      421 agcgacgcgc tcgacgcgct gtacgccgcc gccgacggct atccctactt cgtgcaggcg
      481 tacggcaagg tgacctggga cgtggccgcg tcgagcccgg tgacggccgc cgacgtcagc
      541 gtggccgccc ccgaggcgga gggcgagctg gccgtcggct ttttcggcag caggtacgag
      601 cgggccacgc cggccgagcg cgagtacatg cgcgcgatgg cctcgaccgg ggacgacccg
      661 gtgccgacct cggtcgtggc cgaggaactc gcgcgcaagc cgtccagctt gtcgccagcc
      721 cgggacgggc tgatcaagaa gggactgatc tacagctccg agcgcggcct gatcgcgttc
      781 accgtcccgc acttcggcaa gttcctgcgc tcccagcccg tctaggggtc tcccccggcc
      841 tgacgctggc gtggcctgac gccggccggg tctacccggc catcgcgtcg tctagggaaa
      901 ttcgtagagt cttctagata acggcagact cgcgggtccc ggtgggcccg aggacctcat
      961 cggtatccgc gtcgaggacc gcggcgcgga tcgcgggcct gcgggactgg agatagacgg
     1021 cgaagacgat gtcgaccggc cgcccgcgcg cggaccccgg gtacaccacg aaccgccagc
     1081 acccttcgcg ccagacgtcg gacggcgcgg ccgccgagat ctggcccgcg taccggcgcc
     1141 actgagagct cgcgtgcagc atggtcatgg tccgcccgac cgggaggcgc cgcagctcgc
     1201 agggctcgga gtacccgagc ctgaccatga tctcgccgag ctcgggcgac aggaaggcga
     1261 tgagggtcag ctcgaaggcc agcacggcca gccagagttc ggcctcggcc ggagaggacg
     1321 gcttgcgcag cggcggcagg ccgagggtgg cgacggcgga cgcgcacagc aggccgggcc
     1381 gggcgatggt gcgcagcccg atcggcgtgc cgctgagctc gccgaaacac ccgcagcccg
     1441 agtcggggcg ccgctgtctc atctcgttca gggcgccgac cgcgacgagg aagaagagcg
     1501 cggttcctgt tcgcacgatg gtcgcgggca agc
//
LOCUS       DASPYY010000562         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1358660, whole genome shotgun sequence.
ACCESSION   DASPYY010000562 DASPYY010000000
VERSION     DASPYY010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358660"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..729
                     /locus_tag="VEP73_08925"
     CDS             250..729
                     /locus_tag="VEP73_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYT26586.1"
                     /translation="MASYAEQVGERLRNIRLQKGLSLHDVEERSSKEFKASVLGAYER
                     GERSISVPRLQRLAMFYGVPVDHLLPRLTAAAVEGAAAEEAVCIDLEALESRREPEAR
                     TLGRYLSLIQQQRGDFNGKVLTIRRDDLRVLAAILDQSPGSLVARLSDLGLRRPAYN"
     gene            777..1616
                     /locus_tag="VEP73_08930"
     CDS             777..1616
                     /locus_tag="VEP73_08930"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYT26587.1"
                     /translation="MARPLSLLGCFAHPDDETWSMGGSFALLVPKGVHGAVWTATRGQ
                     AGEIAPGSGATRETLAEVREAEERAAMALVGVADVELGDFTDGGVGDADPADLVAAVQ
                     RALERVRPDVIVTMEPGGVTSHPDHVAVSVAVQAAFAAYAGAARDRGRPAAPGGREPR
                     LYYWGVPSSSLARFRELAAEQGVEIPGEDQPFGPRGTPDERFSCWVDTSPVAEQVWRT
                     LEAHRTQGGDPSRVLLRTGERWREVFASNAFMRVHPAPWPGEPAETSLVDAFTSGDGN
                     GRA"
     gene            1603..2910
                     /gene="mtaB"
                     /locus_tag="VEP73_08935"
     CDS             1603..2910
                     /gene="mtaB"
                     /locus_tag="VEP73_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HYT26588.1"
                     /translation="MAEHDRRGTAAPAPKVAFKTLGCRLNQAESERALERLGARGFDL
                     AHAGEEPDVVVINTCTVTRESTASSRRLIRKTAEAHPGATVVVTGCYAVSDPDVVRAI
                     PGVDLVVDNEGKDRIDELIVGVPRVRRLPLAVREAPLERLRTRVAIKAQTGCDEWCTF
                     CIIPRTRGPLKSYPEDELVADVRRQVDRGVREVVLTGVHLGKYGEDRGEREGLARLLD
                     RLTAIEGLVRLRLSSVLPVQVTPALVERVRDDPKVCRHLHVPLQAGDDEVLGRMHRPY
                     RVAQFLDRVEHAKAEIPGLGLSTDVIVGFPGETAEEFAATMRVTERVGFSKLHVFRYS
                     QRPDTPAAVMPGQVPDGDKKARARELIALGNELRRRFHEAHVGRPLSVLVETAGDALA
                     EGTSDNYIKVRFAGGADLVDRVVAVRGLRADSEGMEGERMETA"
     gene            2907..3254
                     /locus_tag="VEP73_08940"
     CDS             2907..3254
                     /locus_tag="VEP73_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYT26589.1"
                     /translation="MSEANCLFCRVVRKEVPADVVLDTGDVLAFRDINPQAPTHVLVI
                     PKEHLASLEEVADHHGPLLVDLVGAANQVARDEGVAGGFRVVTNIGASAGQSVDHLHL
                     HVLGGRRFGWPPG"
     gene            3294..>4053
                     /locus_tag="VEP73_08945"
     CDS             3294..>4053
                     /locus_tag="VEP73_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYT26590.1"
                     /translation="MRQVAGDGLVAPYGSWRSPISGDLLAGGGIVLDALQPSGTGGLL
                     WLEGRPLEGGRNVVCRWEPGGDPVDLTPPGFDVRTRVHEYGGGAYVVHGDAVIFSNFA
                     DQRLWRQEGDAPPRPLTPEPERPAALRYADGRVTPDGALLVCVRESHDPEGRVVNELV
                     AMPTDGSAAPWVLAGGRDFYASPRFSPDAGQLAWLEWDHPRMPWDGTELQLVGFDPAG
                     GRLRGGPSPVAGGPEESVAQPEWSPDGVLHFISDS"
BASE COUNT          515 a         1490 c         1535 g          513 t
ORIGIN      
        1 gttccggtac ggccagctcg ggtgagccgc ccgcggcggg gtcgggtgac ccgcccgcgg
       61 cggccgccgc ccagggccgc tcaagcggtt tggcggtgat cgtccggtgt catgcacacg
      121 cttcgtgaaa tttcgcctcg cggggtgatt gtgggcgtcg ttcggccgat gctagagtcc
      181 gccacactcc gggtggtcgc ggcgaccacc gccggtcccg caacggcacg cacgccaggg
      241 ggtgggggga tggccagcta cgcggagcag gtgggggagc ggctccgcaa catccggctg
      301 cagaagggcc tgtcgctgca cgacgtcgag gagcggtcgt ccaaggagtt caaggcgagc
      361 gtgctggggg cctacgagcg tggcgagcgg agcatctcgg tcccgcgcct gcagcgcctg
      421 gccatgttct acggcgtgcc ggtcgaccac ctcctgcccc ggctgaccgc cgccgcggtc
      481 gagggcgccg cggccgagga ggccgtctgc atcgacctcg aggcgctcga gtcccggcgg
      541 gagccggagg cgcgcacgct cggccgctac ctgtcgctca tccagcagca gcgcggggac
      601 ttcaacggca aggtgctgac gatccgccgc gacgatctcc gcgtgcttgc cgctatcctc
      661 gaccagtcac cggggagcct ggtggcgcgg ctgtccgacc tcgggctgcg ccgccccgcg
      721 tacaactgac ccgcgacgtc cgacccgggc ggacgctcgc gcagcaggag gtgacgatgg
      781 cgcgcccgct cagcctgctc ggctgcttcg cccaccccga cgacgagacc tggtccatgg
      841 gcggctcgtt cgcgctcctg gtccccaagg gcgtgcacgg cgcggtctgg accgccacca
      901 ggggccaggc gggcgagatc gccccgggct cgggcgccac ccgcgagacg ctggccgagg
      961 tcagggaggc cgaggagcgc gccgcgatgg cgctggtcgg cgtcgccgac gtggagctcg
     1021 gcgacttcac cgacggcggg gtcggcgacg ccgaccccgc cgacctggtc gccgcggtcc
     1081 agcgcgccct cgagcgggtg cggcccgacg tgatcgtgac gatggagccg ggcggcgtga
     1141 cgtcccatcc cgaccacgtg gccgtgtcgg tcgccgtcca ggccgcgttc gccgcctacg
     1201 cgggagcggc cagggaccgc gggcgcccgg cagcgcccgg ggggcgcgag ccgcgcctgt
     1261 actactgggg cgtgccgtcc tcgtcgctgg cgcggttccg ggagctcgcg gccgagcagg
     1321 gcgtcgagat cccaggggag gaccagccgt tcgggcccag gggcacgccc gacgagcgct
     1381 tcagctgctg ggtggacacc tcgccggtgg ccgagcaggt ctggcggacg ctcgaggccc
     1441 accgcaccca gggcggcgac ccgtcgaggg tgctgctgcg caccggcgag cgctggcggg
     1501 aggtcttcgc ctccaacgcc ttcatgcgcg tccacccggc gccctggccg ggcgagccgg
     1561 ccgagacgtc gctcgtggac gccttcacca gcggagacgg caatggccga gcatgaccgg
     1621 cggggcaccg ccgcgcccgc tcccaaggtc gcgttcaaga ccctcgggtg ccggctgaac
     1681 caggccgagt ccgagcgggc gctcgagcgg ctcggcgccc gcgggttcga cctggcccat
     1741 gccggcgagg agcccgacgt ggtcgtcatc aacacctgca ccgtgacccg cgagtcgacc
     1801 gcgtcgtcgc gccggctcat ccgcaagacg gcggaggccc acccgggcgc gacggtggtg
     1861 gtgaccggct gctacgcggt cagcgacccc gacgtcgtgc gggccatccc aggggtcgac
     1921 ctcgtggtcg acaacgaggg caaggaccgc atcgacgagc tgatcgtggg ggtgcccagg
     1981 gtccgccgcc tgccgctcgc ggtccgcgag gcgccgctcg agcggctgcg gacccgggtg
     2041 gcgatcaagg cccagaccgg ctgcgacgag tggtgcacct tctgcatcat cccgcgcacc
     2101 aggggcccgc tgaagtccta tccggaggac gagctggtgg ccgacgtccg ccgccaggtc
     2161 gaccgcggcg tccgggaggt cgtgctcacc ggggtccacc tcggcaagta cggcgaggac
     2221 cgcggcgagc gggaggggct cgcacggctc ctcgaccgcc tcaccgcgat cgaggggctc
     2281 gtccgcctgc ggctctcgag cgtgctgccc gtgcaggtca ccccggccct ggtcgagcgg
     2341 gtgcgcgacg acccgaaggt ctgccgccac ctgcacgtgc cgctgcaggc aggcgacgac
     2401 gaggtcctcg ggcggatgca ccgtccctac cgggtcgccc agttcctcga ccgggtcgag
     2461 cacgccaagg cggagatccc cgggctcggg ctgtccaccg atgtgatcgt cggcttcccg
     2521 ggcgagaccg ccgaggagtt cgcggccacc atgcgggtca ccgagcgcgt cggcttctcc
     2581 aagctgcacg tgttccgcta ctcccagcgg cccgacacgc cggccgcggt catgcccggc
     2641 caggtgcccg acggcgacaa gaaggcgcgc gcccgcgagc tcatcgcgct cggcaacgag
     2701 ctgcgccgcc gcttccacga ggcccacgtc gggcggccgc tctcggtgct cgtggagacg
     2761 gccggggacg cgctcgccga ggggaccagc gacaactaca tcaaggtccg cttcgctggc
     2821 ggcgccgacc tggtcgaccg ggtcgtcgcc gtccgcggcc tgcgggccga cagcgagggc
     2881 atggaggggg agaggatgga gaccgcatga gcgaggcgaa ctgcctgttc tgccgggtcg
     2941 tccgcaagga ggtcccggcc gacgtcgtgc tcgacaccgg tgacgtcctc gcgttccgcg
     3001 acatcaaccc ccaggcgccg acgcacgtcc tggtgatccc gaaggagcac ctcgcgagcc
     3061 tggaggaggt cgccgaccac cacggcccgc tgctcgtcga cctggtcggc gccgccaacc
     3121 aggtcgccag ggacgagggc gtggccggcg gcttccgcgt cgtgaccaac atcggcgcca
     3181 gcgccggcca gtcggtcgac cacctgcacc tgcacgtgct cgggggccgc cgcttcggct
     3241 ggccgcccgg ctgagccggc ggcgcacgca cccggacagt ggagatgccg aagatgaggc
     3301 aagtggcagg cgacgggctg gtggccccct atggctcgtg gaggtccccg atctccggcg
     3361 acctgctcgc cggcggcggg atcgtgctcg acgcgctgca gccgtccggg acgggcggcc
     3421 tcctgtggct cgagggccgg ccgctcgagg gggggcggaa cgtcgtctgc cgctgggagc
     3481 cgggcggcga cccggtggac ctgaccccgc ccggcttcga cgtgcgcacc cgggtccacg
     3541 agtacggcgg cggcgcctac gtcgttcacg gcgacgcggt catcttctcg aacttcgccg
     3601 accagcgcct ctggcgccag gagggggacg ccccgcccag gccgctcacg ccggagccgg
     3661 agcggccggc ggcgctccgc tacgccgacg gcagggtcac cccggacggc gcgctgctcg
     3721 tgtgcgtgcg ggagtcccac gatcccgaag ggcgggtcgt gaacgagctg gtcgcgatgc
     3781 ccaccgacgg gtcggcggcg ccctgggtgc tcgccggcgg ccgggacttc tacgcctccc
     3841 cgcgcttctc cccggacgcc ggccagctcg cctggctcga gtgggaccac ccgcgcatgc
     3901 cctgggacgg caccgagctg cagctcgtcg gcttcgaccc ggcgggcggg cgcctgcggg
     3961 gcgggcccag cccggtcgcg ggcgggcccg aggagtcggt cgcgcagccg gagtggagcc
     4021 ccgacggcgt gctgcacttc atcagcgact cca
//
LOCUS       DASPYY010000563         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1922218, whole genome shotgun sequence.
ACCESSION   DASPYY010000563 DASPYY010000000
VERSION     DASPYY010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922218"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VEP73_08950"
     CDS             complement(<1..321)
                     /locus_tag="VEP73_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26591.1"
                     /translation="MPAARAALLPHPPLLVPDLAGAAAAELDPLRAACRGALASVLAA
                     RPALVVLGDGPVWGLAGPWAVGSFAPYGADLEVAFPGGPLPLDLRGLPEPARLEELPL
                     SLSVA"
     gene            complement(321..1739)
                     /gene="miaB"
                     /locus_tag="VEP73_08955"
     CDS             complement(321..1739)
                     /gene="miaB"
                     /locus_tag="VEP73_08955"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYT26592.1"
                     /translation="MSDRYLIRTFGCQMNEHDSERIAGLLEADGLEPAGGLDDADLVV
                     LNTCAVRENADNKLYGTLGHLATAKARRGLMIAVGGCLAQKDQDKVLARAPWVDVVFG
                     THNLGSLPVLLCRARERGTAQAEFFDEMQMFPSALPARRDSDWAAWVAISVGCNNTCT
                     FCIVPMLRGKERSRQVGDVVAEVQALAGAGVVEVTLLGQNVNSYGTDLPGHRQLFGDL
                     LRALDGVEGLERLRFTSPNPKDFRDDVVDAMASCRPLADHVHFPLQSGSDRVLRLMRR
                     GYRRRRYLEILDRIRAAMPGVAFSTDIIVGFPGETEADFAATLELVERARFDSAFMFQ
                     YSPRPGTEAATMPDRVPPEVVRDRFQRLADLQERISLECNHALVGAEVELLVELASSK
                     TDPTRSSGRTGSNKLCHFPAAPGVSPGDLVTVRVEQAAPHHLVGGRPLTHRPRRVRAA
                     PCGAAPPPRPLPAGAVPLPLVG"
     gene            complement(1764..3344)
                     /gene="rny"
                     /locus_tag="VEP73_08960"
     CDS             complement(1764..3344)
                     /gene="rny"
                     /locus_tag="VEP73_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYT26593.1"
                     /translation="MEAGIIFAVVGTLLGITLGFVGGTTYQRKQSTSLLGSAEEQARA
                     IVDDARRQAETARREAAVEARDQALELRQAAEKEALKLRQEVEVEARGRMAEVQRRED
                     RLLAREEALEAKSSLLDRREASIHDREEALEAARRDLESSADEHRRRLEQVAAMTAPE
                     AKAMLVKQIEEEAKRDAMGLVRDLEQQAREEADRRARKIVTLAIQRVASEQTSESTVA
                     VLPLPSDDMKGRIIGREGRNIRAYEAVTGVNLIIDDTPEAVLLSCFDPVRREVGRLTL
                     EKLVSDGRIHPARIEETYERAKGEVESEVRRAGEDACLEVGLTDLHPELVRVLGRLKY
                     RTSYGQNVLRHLVETAHLAGIMASELGLDVKLAKRCGFLHDIGKALTHEIEGSHAIIG
                     AELARRLKEAPEVVHAIEAHHGEVEPRTVEAVLTQSADAISGGRPGARRETLESYVKR
                     LERLEEIANSYPGVEKVFAMQAGREVRIMVKPEQVDDVAAQVLARDMAKRVEQELQYP
                     GQIKITVVREIRSIEYAK"
     gene            complement(3684..>4121)
                     /locus_tag="VEP73_08965"
     CDS             complement(3684..>4121)
                     /locus_tag="VEP73_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HYT26594.1"
                     /translation="RARGRAELELRLGQRGLPDEAVASALDRLEEVGLVDDAAFAMAY
                     AESRAGRGVDAGAITRELRGRGVDPQLAAQAAGAAVPPDEQAERCRQVAAAKLARMEG
                     LNAEVQFRRLAGYLDRRGYPGELIEHIVRELVEFGEGGGSGRL"
BASE COUNT          531 a         1463 c         1527 g          600 t
ORIGIN      
        1 ggcgaccgac agcgacaggg gcagctcctc gagacgcgcg ggctcgggca ggccccgcag
       61 gtcgaggggc agcgggcctc ccgggaacgc gacctcgagg tcggcgccgt agggggcgaa
      121 ggagccgacc gcccaggggc cggcgagccc ccagaccggg ccgtccccga ggacgacgag
      181 cgcggggcgg gcggccagga ccgacgcgag cgccccccgg caggccgccc gcaacgggtc
      241 cagctcggcc gctgccgcgc cggccaggtc ggggacgagc agcggcgggt gtgggagcag
      301 cgcggctcgg gccgccggca tcagccgacc agaggaagcg ggacggcacc ggccgggagc
      361 ggccgaggtg gcggggcggc gccgcacggc gccgcacgca cccgtcgcgg gcggtgggtc
      421 aggggccgcc cgccgaccag gtgatgcggg gcggcctgct cgacccggac ggtgacgagg
      481 tcgcccgggg agaccccggg cgccgcgggg aagtggcaca gcttgttgct gccggtccgc
      541 ccggagctcc gggtcgggtc ggtcttggag ctggccagct cgacgagcag ctcgacctcg
      601 gcgccgacga gcgcatggtt gcactcgagg ctgatgcgct cctgcaggtc ggcgagccgc
      661 tggaagcggt cgcggaccac ctcgggcggg acccggtccg gcatcgtggc cgcctcggtc
      721 cccggccggg gcgagtactg gaacatgaac gccgagtcga agcgggcccg ctccaccagc
      781 tcgagggtcg cggcgaagtc ggcctcggtc tccccgggga accccacgat gatgtcggtc
      841 gagaacgcca ccccgggcat ggccgcgcgg atccggtcga ggatctcgag gtagcggcgc
      901 cggcggtagc cgcggcgcat caggcggagg acccggtcgg agcccgactg gagagggaag
      961 tggacgtggt cggcgagcgg gcggcagctc gccatggcgt cgaccacgtc gtcccggaag
     1021 tccttcgggt tggggctggt gaagcggagc cgctcgagcc cctccacgcc gtccagcgcc
     1081 cggaggaggt cgccgaagag ctggcggtgc cctggcaggt cggtcccgta ggagttgacg
     1141 ttctgcccga gcagggtgac ctcgaccacc ccggcgccgg cgagcgcctg cacctcggcc
     1201 acgacgtcgc ccacctggcg gctgcgctcc ttgcccctga gcatcgggac gatgcagaag
     1261 gtgcaggtgt tgttgcagcc gacgctgatc gcgacccacg ccgcccagtc gctgtcccgc
     1321 ctggcgggca gcgccgaggg gaacatctgc atctcgtcga agaactcggc ctgggcggtg
     1381 ccccgctcgc gggcccggca cagcaggacc gggaggctgc caaggttgtg cgtgccgaag
     1441 acgacgtcga cccatggtgc cctggcgagc accttgtcct ggtccttctg cgctaggcag
     1501 ccgcccaccg cgatcatgag gccgcgccgc gccttggccg tggccaggtg cccgagggtg
     1561 ccgtacagct tgttgtccgc gttctccctg acggcgcagg tgttcagcac gacgaggtcg
     1621 gcgtcgtcga ggccgccggc cggctcgagc ccgtcggcct cgagcagccc ggcgatgcgc
     1681 tcggagtcgt gctcgttcat ctggcacccg aaggtgcgga tcaggtatcg gtcgctcatc
     1741 tcggctcgtg ggtcgcggtc ggcctacttg gcgtactcga tgctgcggat ctcacggacg
     1801 acggtgatct tgatctgccc ggggtactgc agttcctgct cgacccgctt ggccatgtca
     1861 cgggcgagga cctgggcggc gacgtcgtcg acctgctccg gcttgaccat gatgcgcacc
     1921 tcgcggccgg cctgcatcgc gaagaccttc tccacgccag ggtagctgtt ggcgatctcc
     1981 tcgagccgct cgagccgctt gacataggac tcgagggtct cgcgccgtgc ccccggccgg
     2041 ccgcccgaga tggcgtcggc ggactgggtg aggacggcct ccaccgtgcg tggctcgacc
     2101 tcgccgtggt gggcctcgat ggcgtggacg acctcgggcg cctccttgag ccggcgggcc
     2161 agttcggccc cgatgatggc gtgcgagccc tcgatctcgt gggtcagcgc cttgccgatg
     2221 tcgtgcagga acccgcaccg cttggcgagc ttcacgtcga gcccgagctc ggacgccatg
     2281 atcccggcga gatgggcggt ctcgaccagg tggcggagga cgttctgccc gtacgaggtg
     2341 cggtacttga gccggccgag gacccgcacg agctccgggt gcaggtcggt gaggccgacc
     2401 tcgaggcagg cgtcctcgcc cgccctgcgc acctcgctct cgacctcgcc cttcgcccgc
     2461 tcgtaggtct cctcgatgcg ggccgggtgg atccgcccgt ccgagacgag cttctcgagc
     2521 gtcaggcggc cgacctcgcg ccgcacgggg tcgaagcagg agagcagcac cgcctcgggc
     2581 gtgtcgtcga tgatcaggtt gacgccggtg accgcctcgt aggcgcggat gttgcggccc
     2641 tcccgaccga tgatgcggcc cttcatgtcg tcggacggca ggggcagcac cgccacggtc
     2701 gactcgctgg tctgctcgga ggcgacccgc tggatggcga gcgtgacgat cttccgggcg
     2761 cggcggtccg cttcctcgcg ggcctgctgc tcgaggtcgc gcacgagtcc catcgcgtcc
     2821 cgcttcgcct cctcctcgat ctgcttcacg agcatggcct tggcctcggg cgcggtcatc
     2881 gcggcgacct gctccaggcg gcggcggtgc tcgtcggcgc tcgactcgag gtcgcgcctg
     2941 gccgcctcca gcgcctcctc gcgatcgtgg atgctcgcct cgcgccggtc gagcagggat
     3001 gacttggctt cgagggcttc ctccctggcg agcaggcggt cctcgcggcg ctggacctcg
     3061 gccatgcggc cccgagcctc gacctcgacc tcctgtcgaa gcttgagggc ctccttctcg
     3121 gccgcctgcc tgagctcgag cgcctggtcc cgggcctcga ccgcggcctc gcggcgggcg
     3181 gtctcggcct ggcggcgggc gtcgtcgacg atcgccctgg cctgctcctc ggcggatccg
     3241 agcaggctgg tcgactgctt cctctggtag gtggtgccgc cgacgaaccc cagggtgata
     3301 ccgagcaggg tcccgacgac tgcgaagatg atgccggcct ccacagcggc ctccttagtg
     3361 ctccatccgg ccgggggtac ccggtcggtt caagccgctt cgcggccggg gagccaggag
     3421 ggctcctgag acggcagcag cgccgagcgg ccgcccggcg ctgcagagag cctgcgattt
     3481 acgaccgtgc ggtcgcgtgt aacggaacga tcgagctcaa ggtagaggga gtcctctacc
     3541 ttccggggac ggcaatccgg tagacggtcg attgagcaaa gttacgtaat cgcaggggta
     3601 gctcggcagg accgggccct ctcagagtag gccggcgcag cctgcccggt caaggaaggg
     3661 gcaggtcaga gggttatgaa gggttagagg cggccggagc cgccaccttc cccgaattcg
     3721 acgagttccc tgacgatgtg ctcgatcagc tcaccggggt agccccgccg gtctaagtag
     3781 ccggcgaggc gccggaactg gacctcggcg ttgagcccct ccatgcgcgc cagcttcgcc
     3841 gccgccacct gccggcagcg ttcggcctgc tcatcgggtg gcacggcggc ccccgcggcc
     3901 tgcgcggcga gttgcgggtc gacgccacgc ccgcgcagct cccgggtgat cgcgcccgcg
     3961 tcgaccccgc ggccagcccg gctctcggcg tacgccatgg cgaacgcggc gtcgtcgacg
     4021 agaccgacct cttccagccg gtccagcgcg ctcgccaccg cctcgtccgg cagcccgcgc
     4081 tggccgagcc ggagctccag ttccgctcgg ccgcgcgccc g
//
LOCUS       DASPYY010000564         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1922224, whole genome shotgun sequence.
ACCESSION   DASPYY010000564 DASPYY010000000
VERSION     DASPYY010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922224"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /gene="hisS"
                     /locus_tag="VEP73_08970"
     CDS             <1..1231
                     /gene="hisS"
                     /locus_tag="VEP73_08970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYT26595.1"
                     /translation="VCELFGYHEIRTPIFEQAALFLRAVGQYTDIASKEMYLIEARDG
                     DEAARLALRPEGTAPAMRALIEHALPAQSPLNKLYYIAQNFRYERPQAGRQRQFHQVG
                     IEAVGSDAPALDADVVLVGHEIFRRLGVEATLLLTSLGDAECRPAYRARLLEFLRGLG
                     GDLPAEARARAEQNPLRVLDMKDPLVQELTADAPLMRDALCPACRAHDEAVRGLLADA
                     GLKWVEAPRLVRGLDYYVRTTFEWQAAELAAAQNALGGGGRYDGLSELVGGPPAPGVG
                     WALGLDRVVLALAQAGRLPADPHRLDALVIPLAGAADAAAFTLVRDLRRAGLVADLPY
                     AGRTLKGHMKAAARAGARAVVIIGDAELAAGEAGVRDMQAREQRTVPLAGAVGAVGAV
                     ARRRPPGADPSGPPDLT"
     gene            1257..3071
                     /gene="aspS"
                     /locus_tag="VEP73_08975"
     CDS             1257..3071
                     /gene="aspS"
                     /locus_tag="VEP73_08975"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYT26596.1"
                     /translation="MSRPLLGAMRSHACASLRAADAGTEVRLAGWVARRRDHGGLVFL
                     DLRDASGVVQVVVHPEQAPAAAAAAHAVRTEDCVRVTGMVFPRPAGNENPDLPTGEVE
                     VLASDLEVLGPSATPPFPLEGRAEVDEVLRLRHRYLDLRRAGPARALAARAKFNSVLR
                     RVLEERGFLEVETPYLTRSTPEGARDFLVPTHLRPGEFYALPQSPQLFKQLLMVAGVE
                     RYYQIVRCFRDEALRAERQPEFTQLDIELSFLDEEDVFALGEELMAAAWREVLGVELA
                     TPFPRMSWAEAMRRFGSDKPDLRYGMELVDLGEVFAGTGAGVLGRALETGGSVLGIVV
                     PGGADLTRRQIDALADWAKGRGAGGLAWAAVEPGGLRSPLDKFLSDAERAGLLRATGA
                     EPGDLVLLVADRTLLAQTVLGELRTRLAAERGLVPEGRWEFLWVTDFPMFEWDDAAGR
                     WDAIHHPFTSPAPKWEETFADAPAEAAARAYDLVLNGVELGGGSIRIHRAEVQRKVFD
                     LLGIGQEEAERKFGFLLRGLSFGAPPHGGIAFGLDRVVAVALGAASIREVIAFPKTQS
                     GSDPLTGAPTEVADAQLAEVLLKPLPKPPPRDPPLPSS"
     gene            3366..>3674
                     /locus_tag="VEP73_08980"
     CDS             3366..>3674
                     /locus_tag="VEP73_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYT26597.1"
                     /translation="MAQRRERVPVRTIVTTIGTVLLTAALLLLGWEVRRVLSWIVVAA
                     LLAVVLGPVVDLAERRLHLRRTLATLLVFLLVLLAFAGVLTAFIRPLVKEGGQLADKA
                     P"
BASE COUNT          437 a         1390 c         1354 g          493 t
ORIGIN      
        1 cgtctgcgag ctgttcgggt atcacgagat ccggacaccc atcttcgagc aggcggcgct
       61 cttcctgcgg gcggttgggc agtatacgga cattgccagc aaagagatgt atctgatcga
      121 ggctcgcgac ggggacgaag ctgcgcgcct ggcgctgcgg cccgagggaa ccgcgccggc
      181 gatgcgggcc ttgatcgagc acgcgctgcc cgcacagtca ccgctgaaca aactgtacta
      241 catcgcgcag aatttccgct acgagcggcc ccaggccggc cgccagcgcc agttccacca
      301 ggtcggcatc gaggcggtcg gcagcgacgc acccgccctg gacgccgacg tcgtgctggt
      361 cggccacgag atcttccgcc gcctcggggt cgaggccacg ctgctgctca cgagcctcgg
      421 ggacgccgag tgccggcccg cctaccgcgc gcgcctgctc gagttcctcc gcggcctcgg
      481 cggggacctg cccgccgagg ccagggccag ggccgagcag aaccccctca gggtgctcga
      541 catgaaggac ccgctggtgc aggagctgac cgccgacgcg ccgctcatgc gggacgcgct
      601 ctgcccggcc tgccgggcgc acgacgaggc ggtgcgcggc ctgctcgccg acgccgggct
      661 caagtgggtg gaggcgcccc gcctggtgcg cgggctcgac tactacgtcc ggacgacgtt
      721 cgagtggcag gcggccgagc tcgcggcggc ccagaacgcg ctcggcggcg gcgggcgcta
      781 cgacgggctg tccgagctgg tcggcggccc gcccgccccc ggggtgggct gggcgctcgg
      841 cctcgaccgg gtcgtgctcg cgctggccca ggccggccgg ctgcccgccg acccccaccg
      901 gctggacgcg ctcgtgatcc cgctcgccgg cgccgccgac gccgccgcgt tcacgctcgt
      961 ccgcgacctg cgccgggccg ggctggtggc cgacctgccc tacgccggcc gcaccctgaa
     1021 ggggcacatg aaggcggcgg cgcgggccgg cgcccgggcc gtcgtcatca tcggcgacgc
     1081 cgagctcgcg gccggcgagg ccggcgtgcg cgacatgcag gcgcgcgagc agcgcacggt
     1141 cccgctcgcc ggcgccgtcg gcgccgtcgg cgccgtggcc cgccggcggc cgcccggcgc
     1201 ggatccgagc ggccctcccg acctgacatg acagaacgtg acgtgaggag tcccacgtga
     1261 gccgtcccct gctcggcgcc atgcggtcgc atgcctgcgc cagcctgcgg gccgccgacg
     1321 ccggcaccga ggtgcgcctg gccggctggg tcgcccgccg ccgcgaccac ggcgggctgg
     1381 tcttcctgga cctgcgcgac gcctcgggcg tcgtccaggt cgtcgtccat ccggagcagg
     1441 cgcccgcggc cgccgcggcc gcccacgcgg tccgcaccga ggactgcgtc cgggtgaccg
     1501 gcatggtctt cccccggccc gcgggcaacg agaaccccga cctgccgacg ggcgaggtcg
     1561 aggtcctcgc cagcgacctc gaggtgctcg ggccctcggc cacgccgccg ttcccgctcg
     1621 agggccgcgc cgaggtcgac gaggtgctgc ggctgcgcca ccgctacctg gacctgcgcc
     1681 gcgccggccc ggcgcgggcg ctcgccgcga gggcgaagtt caactcggtg ctgcgccggg
     1741 tcctcgagga gcggggcttc ctcgaggtcg agacgcccta cctgacccgc tcgacgcccg
     1801 agggggcgcg cgacttcctc gtcccgaccc acctgcggcc gggagagttc tacgccctgc
     1861 cccagtcgcc ccagctcttc aagcagctcc tcatggtcgc cggggtcgag cgctactacc
     1921 agatcgtgcg ctgcttccgg gacgaggcgc tgcgggccga gcgccagccc gagttcaccc
     1981 agctcgacat cgagctgtcc ttcctcgacg aggaggacgt gttcgcgctc ggcgaggagc
     2041 tcatggccgc ggcctggcgg gaggtcctcg gcgtcgagct ggccacgccg ttcccgcgca
     2101 tgtcctgggc cgaggccatg cggcggttcg gcagcgacaa gccggacctg cgctacggca
     2161 tggagctcgt cgacctcggc gaggtgttcg ccggcaccgg ggccggcgtg ctcggccggg
     2221 cgctggagac gggcggctcg gtgctcggga tcgtcgtgcc cggcggggcg gacctgaccc
     2281 gcaggcagat cgacgcgctc gccgactggg ccaagggcag gggcgccggg gggctcgcgt
     2341 gggcggcggt cgagccgggg gggctgcggt cgccgctcga caagttcctc tccgacgccg
     2401 agcgggccgg gctcctccgg gcgaccggcg ccgagccggg cgacctcgtg ctgctcgtcg
     2461 ccgaccggac cctcctcgcc cagacggtcc tcggcgagct gcggacccgg ctcgccgccg
     2521 agcgggggct cgtccccgag ggccgctggg agttcctctg ggtcaccgac ttcccgatgt
     2581 tcgagtggga cgacgcggcg gggcgctggg acgccatcca ccaccccttc acgtcgcccg
     2641 cgccgaagtg ggaggagacg ttcgcggacg ctcccgccga ggcggccgcc cgggcctacg
     2701 acctcgtcct caacggcgtc gagctgggcg gcggcagcat ccgtatccac cgggcggagg
     2761 tgcagcgcaa ggtgttcgac ctcctcggca tcggccagga ggaggccgag cgcaagttcg
     2821 gcttcctgct gcgggggctg tccttcggcg cgccgccgca cgggggcatc gcgttcggcc
     2881 tcgaccgggt ggtggcggtg gcgctcgggg cggcctccat ccgcgaggtg atcgcgttcc
     2941 cgaagaccca gtccggctcg gacccgctga ccggcgcgcc caccgaggtg gccgacgcgc
     3001 agctcgccga ggtcctcctc aagcccctgc cgaagccgcc cccccgggat ccccctctgc
     3061 cttcgagcta gcaggaactg actagacccg agttgccggg tcttcgggac ggtggttcaa
     3121 taccactcgc ggcagcgccc gacgttgtac gtgacctggc atttcgtttg cgccaacact
     3181 tcgacgtcgg ggaccgcgct tgcggtcgcg gcaggcaccc ctgaaccggg taccggaaac
     3241 gaccggggcg gtccccaacc tgttttcttc gagagagcag gtgcaaacgt cgaccaggca
     3301 cggcaccgtg gagcgctgcc gtacgcgtcc gggcgggggc gacgagcagg gggacgggag
     3361 gcgtcatggc gcaacggcgg gagcgggtgc ccgtgcgcac gatcgtcacg accatcggga
     3421 cggtcctcct caccgcggcc ctcctcctgc tcggctggga ggtgcggcgc gtcctcagct
     3481 ggatcgtcgt ggccgccctg ctcgccgtgg tcctcgggcc ggtcgtcgac ctcgccgagc
     3541 gccggctgca cctgcgccgg acgctcgcca ccctgctcgt cttcctgctc gtcctgctcg
     3601 cgttcgccgg cgtgctcacc gccttcatcc gccccctggt caaggaaggc gggcagctcg
     3661 ccgacaaggc gccg
//
LOCUS       DASPYY010000565         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_937442, whole genome shotgun sequence.
ACCESSION   DASPYY010000565 DASPYY010000000
VERSION     DASPYY010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937442"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VEP73_08985"
     CDS             <1..251
                     /locus_tag="VEP73_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT26598.1"
                     /translation="VNAQDPEAVALSGLAAEVYEAAPDAVQVGYLATLMRFRRAGAPA
                     LLPSTLGDLGALTGASELVFDAFLTARGLGAWPEEKTA"
     gene            397..>1713
                     /locus_tag="VEP73_08990"
     CDS             397..>1713
                     /locus_tag="VEP73_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HYT26599.1"
                     /translation="MREDVLSLRAMGGDRVSEEYLKAELYHRAEALKDIPDTPLFFGR
                     LDYAEQSADDAIAGHSFHIGRRHVHAPDGTPAVIDWRAPVSRPFYRASATDPMNLALR
                     RRFGFSGGELTAYEDELFGGAEGSSPGRSAPVTATGPSRILIDEIERPRSGPMRDIVA
                     TIQPDQDDIVRADAAQTVCVQGAPGTGKTAVGLHRVAYLLYAYREQVRRRGVVMVGPN
                     QAFLSYIRNVLPALGELDVSQTTVGELVGSVQVRGSDDEAAAIVKGDARMAQVLRRAL
                     WASVRAPSDAVVLPRGSRRWRVAAWEIEELVHELRHRGVRYGAARELLEHRIAHVILT
                     MMEAAGEACDDRTHEAVRRSRPVRAAVDAIWPKVDAVRLVFGLLSSAEALAAAADGVL
                     TAGEQAAIVWPSPPRGPGSARWSAADAVLIDEARDLIGRTPSLAHVV"
BASE COUNT          222 a          626 c          630 g          235 t
ORIGIN      
        1 tggtcaacgc ccaggatccg gaggccgtcg cgctgtccgg gctcgcggcc gaggtgtacg
       61 aggccgcgcc ggacgccgtc caggtcggct acctggccac cctgatgcgg ttccgccggg
      121 ccggggcgcc cgccctgctg ccgtccaccc tgggtgacct cggcgccttg accggcgcct
      181 ccgagctggt cttcgacgcc ttcctcaccg cgcgcggcct cggcgcctgg cccgaggaga
      241 aaaccgcgtg acttcccggt gactacctgg taagttcccg gccttgtggc tgcgtcacaa
      301 gcacaagaaa atcatcatgc cgaacggtcc ggcggcgacc tcgtcctgac cgaagaacgc
      361 gaccaactgc ggagctcacg cgagttcctg cacctcatgc gcgaggacgt cctgtcgctg
      421 cgggcgatgg gcggcgaccg ggtctccgag gagtacctca aggccgagct gtaccaccgg
      481 gccgaagccc tcaaagacat ccccgacacc cccctcttct tcggccgcct cgactacgcc
      541 gaacagtccg cagacgacgc catcgccggg cacagcttcc acatcggccg caggcatgtg
      601 cacgcgccgg acggcacgcc cgcggttatc gactggcggg cgccggtgtc gcggccgttc
      661 taccgggcca gcgcgaccga cccgatgaat ctggcgctgc ggcggcgctt cgggttctcc
      721 ggcggcgagc tgaccgccta cgaggacgag ctcttcggtg gtgccgaggg cagcagcccg
      781 ggccgttcgg cccctgtcac ggccaccggg ccgagccgga tcctgatcga cgagatcgag
      841 cggccgcggt cgggacccat gcgggacatc gtcgcgacca tccagcccga ccaggacgat
      901 atcgtgcgcg ccgacgcggc gcagaccgtc tgcgtgcagg gcgcgcccgg caccgggaag
      961 accgcggtcg ggctgcaccg ggtcgcgtac ctgctgtacg cgtaccgcga gcaggtccgg
     1021 cggcgcggcg tggtcatggt cgggccgaac caggccttcc tctcctacat ccgcaacgtc
     1081 ctgccggcgc tcggcgagct cgacgtcagc cagaccaccg tcggcgagct ggtcggctcg
     1141 gtccaggtcc gcggctcgga cgacgaggcg gccgcgatcg tcaagggcga cgcgcggatg
     1201 gcgcaggtgc tgcgacgggc actgtgggcc tcggtccgcg cgccgtcgga cgcggtggtg
     1261 ctgccgcgcg gctcgcggcg ctggcgggtg gccgcctggg agatcgagga gctcgtgcac
     1321 gagctgcggc accgcggcgt ccgctacggc gcggcccgcg agctgctcga gcaccggatc
     1381 gcgcacgtga tcctgaccat gatggaggcc gcgggggagg cctgcgacga ccgcacccac
     1441 gaggcggtgc ggcggtcgcg cccggtgcgg gccgcggtgg acgcgatctg gccgaaggtt
     1501 gacgctgtcc ggctggtgtt cggcctgctg tcctcggcgg aggccctggc ggcggcggcc
     1561 gatggggtgc tgaccgccgg cgagcaggcc gcgatcgtgt ggccgtcgcc gccccgcggc
     1621 cccggctcgg cccgctggtc cgcggcggac gccgtgctca tcgacgaggc gcgtgacctg
     1681 atcggccgga cccccagcct ggcgcacgtg gtg
//
LOCUS       DASPYY010000566         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2483080, whole genome shotgun sequence.
ACCESSION   DASPYY010000566 DASPYY010000000
VERSION     DASPYY010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483080"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VEP73_08995"
     CDS             complement(<1..573)
                     /locus_tag="VEP73_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HYT26600.1"
                     /translation="MRKTSIRLADGRELIYYDDEHPDADRSMPDRRDPSRIETRSELR
                     RDPLLDEWVAVASHRQDRTYLPPPDECPLDPSTPGRLTEIPAPDYDVVVFENRFPSLA
                     LDVDRRAGRARGPAPGQAAPDGTGQAAPDGLVPRRPGIGRCEVVCFTSDHDGALGRLP
                     ASRVRTVVDVWADRTVELGAMPGIEQVFPFE"
     gene            728..3058
                     /gene="pcrA"
                     /locus_tag="VEP73_09000"
     CDS             728..3058
                     /gene="pcrA"
                     /locus_tag="VEP73_09000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HYT26601.1"
                     /translation="MDTLPLPDSHAVGPRASGPQASADLLEGLNDAQRRAVLSTEGPT
                     LIVAGAGSGKTRVLTHRVAHLMRDLGVHPANLLAITFTNKAAREMQERLRHLVGPVVK
                     AMWVSTFHSACARILRRSGSRLGYSSTFTIYDEADAERLMTAVCRQLDFDPKRLAPRA
                     VRHAVSAAKDELRDPSGYADIAMTWFDKKVAEAYALYQRRLEESNALDFDDLIMQTVR
                     LFTEHPDVLAEYQARFRYVLVDEFQDTNVAQYELLKLLVAKSRNLAVVGDSDQSIYAF
                     RGATIRNILDFEADYPETNVILLEQNYRSTQTILSAANAVIANNLERKPKSLWTLLDG
                     GYPIVRYQAENEHDEAAWLVGEVDRLHEHGTAYGQVVVFYRTNAQSRVLEEVFVKAGL
                     PYKVVGGVKFYDRREVKDLLAYLKATVNPLDTVSLRRVLNVPRRGIGETSERAIDRFA
                     AEEGIPFAEALRRAGEVPGLGARQGRAVGDFVAVLDELRTGMEDGRTPSQMVEDAWSL
                     SGLMRELEADDTIEGQGRVENVRELRSVAEEFAEREPDGGLPQFLESIALVSDADQVE
                     SGSQAVTLMTLHTAKGLEYPVVFIVGLEENVFPHARSIGEPRELEEERRLAYVGITRA
                     RERLYLTNAWSRSLFGATNYNLPSRFLKEVPDELVTVAAVEPRRRSEPAATWGDTSPA
                     FGAGRRSFGPGGGAPPAGASWNRPATPAAQLADQQRGALDLGLVAGDAVVHRQWGEGR
                     VVTISGQGERAMAEVDFPSQGRKRLLLRYAPLRRPG"
BASE COUNT          461 a         1207 c         1167 g          422 t
ORIGIN      
        1 ctcgaacggg aacacctgct cgatccctgg catggcgccg agctcgaccg tccggtcggc
       61 ccacacgtcc accaccgttc gcacccggct cgccggcagg cggccgagcg ccccgtcgtg
      121 gtcgctggtg aagcagacca cttcgcagcg gccgatgcca ggccggcggg ggaccaggcc
      181 gtccggcgcg gcctgccccg tgccgtccgg cgcggcctgc cccggcgccg gcccgcgagc
      241 ccgcccggcg cgccggtcga cgtcaagggc cagcgagggg aagcggttct cgaacacgac
      301 gacgtcgtag tcgggcgccg ggatctcggt gagccgcccg ggcgtggacg ggtccagcgg
      361 gcactcgtcg ggcgggggca ggtaggtgcg gtcctggcgg tgggaggcga ccgccaccca
      421 ctcgtcgagc agcgggtcgc ggcggagctc ggagcgggtc tcgatcctgg acgggtcgcg
      481 ccggtcgggc atgctccggt cggcgtccgg gtgctcgtcg tcgtagtaga tcagctccct
      541 gccgtcggcc agccggatgc tggtcttgcg cacggtcgac gggaggctca tgcggccctc
      601 gcgggcccga gcgccgccag cagctccggc tggtgggcgc ggagccactc cctttccatg
      661 gcgctcctgt ctaccggcgg aatgccgaga gcgtacctcg ttgggtggta cccatatact
      721 cggggcgatg gacacgctcc cgctcccaga ctcccatgcc gtgggcccgc gcgcctcggg
      781 cccgcaggcg tcggccgacc tcctcgaggg cctgaacgac gcccagcgcc gtgccgtgct
      841 ctcgaccgag ggcccgaccc tgatcgtggc cggcgccggg tcgggcaaga cccgcgtgct
      901 cacccaccgg gtcgcccacc tgatgcggga cctcggcgtc cacccggcca acctgctcgc
      961 gatcaccttc accaacaagg ccgccaggga gatgcaggag cgcctgcgcc acctggtcgg
     1021 gccggtggtc aaggccatgt gggtgtcgac cttccactcc gcgtgcgcgc gcatcctccg
     1081 ccggagcggc tcccgtctcg ggtactcctc caccttcacg atctacgacg aggccgacgc
     1141 cgagcgcctg atgacggccg tctgccggca gctcgacttc gaccccaagc gcctggcccc
     1201 gcgggccgtc cgccacgcgg tcagcgcggc aaaggacgag ctgcgcgacc cctccggcta
     1261 cgccgacatc gcgatgacct ggttcgacaa gaaggtcgcc gaggcgtacg cgctctacca
     1321 gcgccggctc gaggagtcga acgcgctcga cttcgacgac ctcatcatgc agaccgtccg
     1381 cctgttcacc gagcaccccg acgtgctcgc cgagtaccag gcgaggttcc gctacgtgct
     1441 cgtcgacgag ttccaggaca ccaacgtcgc ccagtacgag ctgctgaagc tgctcgtggc
     1501 caagagccgc aacctcgccg tggtggggga ctcggaccag agcatctacg cgttccgggg
     1561 tgccacgatc cgcaacatcc tcgacttcga ggccgactac cccgagacga acgtgatcct
     1621 gctcgagcag aactaccgct ccacccagac gatcctctcg gcggccaacg cggtgatcgc
     1681 caacaacctc gagcgcaagc ccaagagcct gtggaccctg ctcgacgggg ggtatccgat
     1741 cgtccgctac caggccgaga acgagcacga cgaggcggcc tggctcgtcg gcgaggtcga
     1801 ccgcctccac gagcacggga cggcgtacgg gcaggtggtc gtcttctacc ggaccaacgc
     1861 ccagtcccgg gtcctggaag aggtgttcgt caaggcgggc ctgccgtaca aggtggtcgg
     1921 cggggtcaag ttctacgacc ggcgcgaggt caaggacctg ctcgcctacc tcaaggcgac
     1981 ggtgaacccg ctcgacacgg tgagcctccg ccgcgtgctc aacgtgccga ggcgcgggat
     2041 cggcgagacc agcgagcgcg ccatcgaccg cttcgcggcc gaggagggca tcccgttcgc
     2101 cgaggcgctg cggcgggccg gcgaggtgcc cggcctgggc gcgcgccagg gccgggccgt
     2161 cggcgacttc gtggccgtcc tcgacgagct gcgcacgggc atggaggacg gccggacgcc
     2221 cagccagatg gtggaggacg cctggtcgct gtccggcctc atgcgcgagc tggaggccga
     2281 cgacaccatc gagggccagg gccgcgtcga gaacgtgcgc gagctccggt cggtcgccga
     2341 ggagttcgcc gagcgggagc cggacggcgg cctgccccag ttcctcgagt cgatcgcgct
     2401 ggtgagcgac gccgaccagg tcgagagcgg cagccaggcg gtgaccctga tgacgctcca
     2461 cacggccaag gggctcgagt acccggtggt gttcatcgtc gggctcgagg agaacgtgtt
     2521 cccgcacgcc cgctcgatcg gcgagccgcg cgagctcgag gaggagcggc ggctcgccta
     2581 cgtcggcatc acccgcgccc gcgagcgcct ctacctcacc aacgcctgga gccggtcgct
     2641 gttcggcgcc acgaactaca acctgccctc gcggttcctc aaggaggtgc cggacgagct
     2701 ggtcacggtc gccgcggtcg agccgaggcg ccgctcggag ccggcggcca cctggggcga
     2761 cacgagcccc gcgttcgggg ccggccgccg gtcgttcggc cccggcggcg gcgcgccgcc
     2821 cgccggggcc tcgtggaacc gcccggcgac ccccgccgcc cagctcgccg accagcaacg
     2881 gggcgcactc gacctcggcc tcgtggccgg cgacgcggtc gtccaccgcc agtggggcga
     2941 aggccgggtc gtgaccatct ctggccaggg ggagcgtgcc atggccgagg tcgacttccc
     3001 gagccagggc cgcaagcgcc tgctgctccg ctacgccccg ctgcgccggc ccggctgagc
     3061 tcccgacata caggggccgg aacctcccga cacaggcgcg gcgcccgcca cccggcgggc
     3121 gccgcgctgt caggcggtgt gtgcggtcgg gcgtcagtcg tggcgtcccg ccgtgcgccg
     3181 ggcggagcgc agcgccagga gcccgccgac gacgagcacc aggccggcga cgaggatcgg
     3241 cacgctgttg gagccgg
//
LOCUS       DASPYY010000567         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1640221, whole genome shotgun sequence.
ACCESSION   DASPYY010000567 DASPYY010000000
VERSION     DASPYY010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640221"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1013
                     /locus_tag="VEP73_09005"
     CDS             57..1013
                     /locus_tag="VEP73_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYT26602.1"
                     /translation="MATVLVLVEHEEGTPKKVTFEMLAKARELGEPAAVFVGRDLGAA
                     AGALGAHGAATVYAAAGEGWTNALSGPVVDALEAAAERAEPAAVLVAATPTGKDAAAR
                     LGARRGWGVLADVVDLDADLVATQVVFGGATVVRSRVTTGTPVLLVKPNSFTVGEVGG
                     PPPEVVDLGTERRSAATDGRVTDRVVSERGGRPDVAEAEIVVSGGRGLQDPSNFALVE
                     ALADVLGAAVGASRAAVDAGWYPHQHQVGQTGKTVSPQLYVAVGISGAIQHRAGMQTS
                     KTIVAVNKDGDAPIFAIADFGVVGDLFKVVPRLTDEVASRKG"
     gene            1022..4333
                     /locus_tag="VEP73_09010"
     CDS             1022..4333
                     /locus_tag="VEP73_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYT26603.1"
                     /translation="MPPEEGPPRRSDAHPTASGAARSCLRAGGRWARLLAGRPGRRAR
                     LRAARTALGAEVGEHLRDGPAPMFLAMPLAIHRAAAAELAAGGAAAPAAAGAVRRRLA
                     ARRMRAADAQLGAWASGLADPPAPELAAAHRAAGRAGRAVDAAEAELAASRRAALASA
                     RLTAALAVQAVADVVVWTAAGLASGVGMIRAWLVSLWVRSARRLVLARRATVSRLVAA
                     RDRRRGRRAATRRDLPSAGRHGPPGRHERDALGWRDAMSEARRSEARPGVPNGTEQGV
                     PNGTGRSVPSGKENPDGSGGEAGSGAPPGGAQYWWTPGEPEDEPPGRAGREAAAAHAT
                     TVRLPALPDAAWNGHAAATRPRDPVLAAVSGFAAAVARPVRRFGRRTARWLAPLVVAV
                     AAPPNRARRPWAALIRPVVAACALSLVLGMFTALPTGVVMAGSVKAAGKGLPDLADLR
                     PLDQPERTEVFDVNGRLIEVLKYDQDRIVVPLEAVPATVQSAVVAAEDARFYEHRGVD
                     QRGIMRALFADLSRGEDAQGGSTITQQLVRNAYPHLKERSLVRKVREAALAAELERKQ
                     SKPKILEQYLNRVYFGGGYYGIEAASLGYFYRHVGQLSLAQAALLAGVIRSPETANPR
                     ANPTEALRLRDSVLDRMVELGMEPADLAARARREKLGVKKRKELPNGRYRWFLDGVKQ
                     QLLEDPRLGRTWDEREKKLYQGGLRIYTTLNPKLQADAEQAVRRRGPTVAGPDVALVA
                     MIPSTGAVRAVVGGRDLEHDRFNLALQGHRQAGSSFKPFVLASALENGISPDSVWGTS
                     GWAPRKVCGVPWGVSNYEGQAAGQLSVRVATWHSVNGVYGRLMEQLCPAKVVDMARRL
                     GVRVAQRNEQAPSIALGSAEVYPIDMAAAYATFADMGVYHKPNFVTRVVRHGQLVLEN
                     NEAGERRIPATLAYEVNDVLKGVISQGTGTAAQIGRPEAGKTGTTQDYHDAWFVGYTP
                     QLATAVWMGYPDRQRAMRNIGGVRTVVGGTFPAGIWHDFMQEALADTQPLDWQRPEGQ
                     LPYTVLPPPPPATTTTIVPGQPQHGPKKEPGRRGPGSTLPPQTIPPIFGTQTTVVQGA
                     PVEPRRPAGAYHRRK"
     gene            4342..>4702
                     /locus_tag="VEP73_09015"
     CDS             4342..>4702
                     /locus_tag="VEP73_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYT26604.1"
                     /translation="MPYLDYAASAPLRAEALAAMRPFLERPAANPSSQHGPGRAARAA
                     VETAREQVAALVGAAPTEVVFTSGGTEADNLAVKGTVLAREPLARHLVASAVEHHAVL
                     EAAAWAVADAGADLALAP"
BASE COUNT          597 a         1822 c         1752 g          531 t
ORIGIN      
        1 gctggccggc ttcctcgccg cgaacaagtt catctgaccc gggaggcgtg aggagcatgg
       61 caacggttct cgtcctcgtg gagcacgagg agggcacgcc gaagaaggtc accttcgaga
      121 tgctcgccaa ggctcgtgag ctgggcgagc cggccgcggt gttcgtcggc cgggacctcg
      181 gcgccgccgc cggcgccctc ggcgcccacg gcgcggcgac cgtctacgcg gcggccggcg
      241 agggctggac gaacgcactc agcgggcccg tggtggacgc cctggaggcg gccgccgagc
      301 gggccgagcc ggccgccgtc ctcgtggccg cgacccctac cggcaaggac gccgccgccc
      361 ggctcggcgc ccgcaggggc tggggggtgc tcgccgacgt ggtcgacctc gacgccgacc
      421 tggtcgccac ccaggtcgtg ttcggcgggg cgaccgtggt gcggtcccgg gtcacgaccg
      481 gcaccccggt cctcctggtg aagcccaact ccttcacggt cggggaggtg ggcgggccgc
      541 ctcccgaggt cgtcgacctc gggaccgagc gccgctccgc cgcgaccgac ggccgcgtca
      601 ccgaccgcgt cgtctccgag cggggcggcc gcccggacgt cgccgaggcc gagatcgtgg
      661 tctcgggcgg ccgcggcctg caggacccgt ccaacttcgc gctggtggag gcgctggcgg
      721 acgtgctcgg ggccgcggtc ggggccagca gggccgccgt cgacgccggc tggtacccgc
      781 accagcacca ggtcgggcag accggcaaga ccgtctcccc gcagctctac gtcgcggtcg
      841 gtatctccgg ggcgatccag caccgggccg ggatgcagac ctccaagacc atcgtcgccg
      901 tcaacaagga cggcgacgcg ccgatcttcg ccatcgccga cttcggcgtg gtgggcgacc
      961 tcttcaaggt cgtcccgcgc ctcaccgacg aggtcgcctc gcggaagggg tagccgggcc
     1021 gatgccaccc gaggaaggac cgccacggcg cagcgacgcc catcccaccg cctcaggtgc
     1081 ggccaggtcc tgcctgcgtg ccggcggccg ctgggcccgc ctgctcgccg gccggccggg
     1141 gcgccgggcg cggctgcggg ccgcccggac cgcgctcggc gcggaggtcg gcgagcacct
     1201 gcgcgacggg ccggcaccga tgttcctggc catgccgctg gcgatccacc gggcggcagc
     1261 ggccgagctg gccgcgggcg gagcggccgc ccccgcggcc gccggggcgg tgcgccggcg
     1321 cctggccgcg aggcgcatgc gtgcggccga cgcccagctc ggcgcctggg cgtcagggct
     1381 cgccgacccg cccgcgcccg agctggccgc ggcccaccgg gcggccgggc gcgccgggcg
     1441 ggccgtcgac gccgccgagg cggagctggc cgcctcgcgg cgtgcggcgc tggcctccgc
     1501 gaggctgaca gccgccctcg ccgtccaggc ggtcgccgac gtcgtggtct ggacggccgc
     1561 ggggctcgcg agcggtgtcg gcatgatccg cgcatggctg gtctcgctgt gggtgcgctc
     1621 tgcccggcgc ctcgtcctcg cccgccgggc gaccgtgtcc cggctggtcg ccgcccgcga
     1681 ccgtcggcga ggccgccggg cggcgacgcg ccgggacctg ccgtcggcgg gccggcacgg
     1741 gccgccgggc aggcacgagc gcgatgcgtt gggatggagg gacgccatgt cggaggcacg
     1801 ccgctcggag gcacgccccg gcgtgccgaa cgggacagag caaggcgtgc cgaacgggac
     1861 aggcagaagc gtgccgagcg ggaaagagaa cccggacggg tccggcggcg aggcgggctc
     1921 cggcgcgccg ccgggcggcg cccagtactg gtggacgcct ggcgagcccg aggacgagcc
     1981 tcccgggcgg gccggccggg aggcggccgc tgcgcatgcc acgaccgtcc gcctgccggc
     2041 gctgccggac gcggcgtgga acggccacgc cgcggccacg cggccacgcg acccggtgct
     2101 ggccgccgtg agcggcttcg ccgccgccgt ggcgcgcccg gtccgccgct tcggccggcg
     2161 gaccgcccgg tggctcgcgc ccctcgtcgt cgccgtggcc gcaccgccca accgcgctcg
     2221 ccggccctgg gcggcgctca tccggccggt ggtcgccgcc tgcgcgctct ccctcgtcct
     2281 cggcatgttc accgcgctgc ccaccggcgt ggtgatggcc ggctcggtca aggcggccgg
     2341 caagggtctg cccgacctgg ccgacctccg cccgctcgac cagcccgagc ggaccgaggt
     2401 cttcgacgtc aacggcaggc tcatcgaggt gctcaagtac gaccaggacc gtatcgtggt
     2461 gccgctcgag gcggtccccg cgacggtcca gagcgcggtg gtcgccgccg aggacgcccg
     2521 cttctacgag caccggggcg tcgaccagcg cggcatcatg cgcgcgctct tcgccgacct
     2581 gagcaggggg gaggacgcgc agggcggcag caccatcacc cagcagctcg tccgcaacgc
     2641 ctacccgcac ctcaaggagc gctcgctggt ccgcaaggtc agggaggccg ccctcgccgc
     2701 cgagctggag cgcaagcaga gcaagccgaa gatcctcgag cagtacctga accgggtcta
     2761 cttcggcggc gggtactacg ggatcgaggc ggccagcctc gggtacttct acaggcacgt
     2821 cggccagctc tccctggccc aggcggccct gctcgccggc gtcatccgct cgccggagac
     2881 cgccaacccg cgcgccaacc ccaccgaggc gctgcgcctg cgcgacagcg tgctcgaccg
     2941 catggtcgag ctcggcatgg agcccgccga cctggccgcg cgggcccgcc gggagaagct
     3001 cggcgtgaag aagcgcaagg agctgccgaa cggcaggtac cgctggttcc tggacggcgt
     3061 caagcagcag ctcctcgagg acccgcggct cggccgcacc tgggacgagc gcgagaagaa
     3121 gctctaccag ggtggcctgc gcatctacac gaccctcaac ccgaagctcc aggccgatgc
     3181 cgagcaggcg gtgcggcgcc gcggcccgac ggtggccgga ccggacgtcg ccctcgtcgc
     3241 gatgatcccc tcgaccggcg cggtgcgggc cgtcgtgggc ggccgcgacc tcgagcacga
     3301 ccgcttcaac ctcgccctcc agggccaccg ccaggccggc tcgtcgttca agccgttcgt
     3361 gctcgcctcc gcgctcgaga acggcatctc ccccgactcg gtctggggca cgagcggctg
     3421 ggcgccccgc aaggtctgcg gcgtgccgtg gggggtcagc aactacgagg gtcaggccgc
     3481 cgggcagctg tcggtgcgcg tcgcgacctg gcactcggtc aacggcgtct acggccgcct
     3541 gatggagcag ctctgcccgg ccaaggtggt ggacatggcg cgccggctcg gggtgcgggt
     3601 ggcccagcgg aacgagcagg cgccgtcgat cgcgctcggc tcggccgagg tctaccccat
     3661 cgacatggcg gccgcctacg cgaccttcgc cgacatgggc gtctaccaca agccgaactt
     3721 cgtcaccagg gtcgtgcgcc acggccagct ggtgctcgag aacaacgagg cgggcgagcg
     3781 tcgcatcccc gccaccctcg cctacgaggt caacgacgtg ctcaagggcg tcatcagcca
     3841 ggggaccggc actgccgccc agatcggccg gccggaggcc ggcaagaccg gcacgaccca
     3901 ggactaccac gacgcctggt tcgtcggcta cacgccccag ctcgccaccg ccgtctggat
     3961 gggctatccc gacaggcagc gggccatgcg gaacatcggc ggggtccgca cggtcgtcgg
     4021 cggcacgttc ccggccggga tctggcacga tttcatgcag gaggcgctcg ccgacaccca
     4081 gccgctcgac tggcagcgcc cggaaggcca gctcccctac accgtcctgc cgccgccacc
     4141 gccggccacc accacgacca tcgtccccgg ccagccccag cacggcccga agaaggagcc
     4201 gggccggagg gggccgggct cgaccctgcc gccgcagacc atcccgccga tcttcgggac
     4261 gcagaccacc gtcgtgcagg gtgccccggt cgagccgagg cgcccggccg gcgcctacca
     4321 ccggaggaag tagcaggtcc cgtgccctat ctcgactacg cggccagcgc cccgctccgt
     4381 gccgaggcgc tcgccgccat gcgcccgttc ctcgagcggc cggccgcgaa cccgtcgagc
     4441 cagcacggcc cgggccgggc agcccgggcc gcggtcgaga ccgcccggga gcaggtggcc
     4501 gccctggtcg gggcggcccc gaccgaggtc gtgttcacct cgggcggcac cgaggccgac
     4561 aacctggccg tcaagggcac cgtgctcgcc cgcgagccgc tggcccgcca cctggtcgcc
     4621 tcagcggtcg agcaccacgc cgtgctcgag gcggccgcct gggcggtggc cgacgccggc
     4681 gccgacctcg cgctcgcccc gg
//
LOCUS       DASPYY010000568         1793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1359362, whole genome shotgun sequence.
ACCESSION   DASPYY010000568 DASPYY010000000
VERSION     DASPYY010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359362"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..877
                     /locus_tag="VEP73_09020"
     CDS             71..877
                     /locus_tag="VEP73_09020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYT26605.1"
                     /translation="MGDIGGDRGAGALAALAAALGAGEVGVVDLTQPLSEATPLLRLP
                     EPFANTPPFRLRELSRYDERGPAWYWNAFEGGEHTGTHFDAPVHWVTARGGDDVAQVP
                     PGRLVGPAVVIDKAAEAAKDPDYLLTIDQVEAFEAEHGPLPAGGWLLYRTGWDARAHD
                     EAAFLNADERGAHTPGVAADCARWLAEDRPILGLGVETVGTDAGAAPGFDPPFPCHSF
                     LLGAGKYGLTQLANLARLPATGAVVIAAPLKIVGGSGSPCRVLALVPASG"
     gene            complement(912..>1793)
                     /locus_tag="VEP73_09025"
     CDS             complement(912..>1793)
                     /locus_tag="VEP73_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYT26606.1"
                     /translation="GIALAARWLARHPERYRAWVERQLERPLIARVRVRRRRELEFLV
                     RRFKPSGALGLSLTLGVVALVAASAAFALILANVRGGKELALVDTPTYQFFLDHREPW
                     VTTALGAVTHLGDPLVVTGLAVAVGLAWRWRGGTWRPLVLLLGARLGAVACWWTLKAF
                     VPRPRPPASLALTGVEGTAFPSAHAVDAAAVFGMLAALAAASAPLWTRKVTAWALALA
                     AVAVIGLSRLYLGASWLTDVLGGGALGAGWMLAVLTTAQTLGGLLSARQQHPDADARE
                     PSLVPGDPFPSRRPPPG"
BASE COUNT          227 a          674 c          719 g          173 t
ORIGIN      
        1 cgcgcgggtt cgtgtcgagg ctccgcgggg cgcgcccgct cctcgcgccg ttcgagagag
       61 ggagggtcgt atgggcgaca tcggtggcga ccggggagcc ggcgcgctgg ccgcgctggc
      121 ggcggcgctc ggggcggggg aggtcggcgt cgtcgacctg acccagccgc tgtcggaggc
      181 gacgccgctg ctgcgcctgc ccgagccgtt cgcgaacacg ccgccgttcc ggctgcggga
      241 gctcagccgc tacgacgagc gcggcccggc ctggtactgg aacgcgttcg agggcgggga
      301 gcacaccggc acgcacttcg acgcgccggt ccactgggtc accgcccgcg gcggcgacga
      361 cgtcgcgcag gtcccgcccg gccggctggt cggcccggcg gtggtgatcg acaaggccgc
      421 cgaggcggcc aaggaccccg actacctgct cacgatcgac caggtggagg cgttcgaggc
      481 cgagcacggc ccgctgcccg ccggcggctg gctgctctac cgcaccgggt gggacgcccg
      541 cgcccacgac gaggcggcgt tcctcaacgc cgacgagcgg ggggcgcaca ccccgggcgt
      601 cgcggccgac tgcgcccggt ggctcgccga ggatcggccg atcctcgggc tcggcgtcga
      661 gacggtcggc accgacgccg gcgcggcgcc cggcttcgac ccgccgttcc cgtgccacag
      721 cttcctgctc ggggccggca agtacggcct gacgcagctc gcgaacctgg cccgcctccc
      781 ggcgacgggc gcggtggtga tcgccgcgcc gctgaagatc gtcggtggct cgggcagccc
      841 gtgccgggtg ctcgccctcg tgcccgcgtc cggctgaggc cggcccggcc cgcatggcga
      901 agccggcgcg ctcatccggg cggagggcga cgcgacggga acgggtcgcc gggcacgagg
      961 gacggctccc gcgcgtccgc gtcggggtgc tgctgccggg ccgacagcag cccgccgagg
     1021 gtctgggccg tggtcagcac ggccagcatc cagccggcgc cgagcgcgcc gccgccgagc
     1081 acgtcggtga gccagctcgc cccgaggtag aggcgggaga gcccgatgac cgcgacggcc
     1141 gccagcgcga gcgcccacgc ggtcaccttg cgggtccaca gcggcgccga ggcggccgcc
     1201 agcgcggcga gcatgccgaa cacggcggcg gcgtcgaccg cgtgcgcgga cgggaacgcg
     1261 gtcccctcca cgccggtgag cgcgagggac gccggcggcc ggggccgggg gacgaacgcc
     1321 ttcagcgtcc accagcacgc caccgcgccg agccgggcgc cgagcagcag gaccagcggc
     1381 cgccacgtgc cgccccgcca gcgccacgcc aggccgaccg cgaccgccag gccggtcacc
     1441 accagcgggt cgcccagatg ggtgaccgcg ccgagcgcgg tggtgaccca cggctcccgg
     1501 tggtcgagga agaactggta ggtgggcgtg tcgacgagcg ccagctcctt gccgccacgc
     1561 acgttggcga ggatcagcgc gaaggcggcc gaggcggcga cgagggcgac caccccgagg
     1621 gtgagcgaca gtccgagcgc cccggacggc ttgaaccggc ggaccaggaa ctcgagctcg
     1681 cggcgacggc ggacccggac gcgggcgatg aggggccgct cgagctggcg ctcgacccac
     1741 gcccggtacc gctcggggtg gcgggcgagc cagcgcgcgg ccagggcgat gcc
//
LOCUS       DASPYY010000569         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_518889, whole genome shotgun sequence.
ACCESSION   DASPYY010000569 DASPYY010000000
VERSION     DASPYY010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..237)
                     /locus_tag="VEP73_09030"
     CDS             complement(10..237)
                     /locus_tag="VEP73_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26607.1"
                     /translation="MPAGWSQGVLTVAESFAKARFLTVQEVAKLMRVSSMTVYRLIKA
                     GDLPAVRVGRSFRVRDVDVDAYLESRYTQAG"
     gene            complement(328..930)
                     /locus_tag="VEP73_09035"
     CDS             complement(328..930)
                     /locus_tag="VEP73_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYT26608.1"
                     /translation="MTLARRFVSFLSDYGHSDEFVGVCKSVMLSLAPDLQIIDITHDL
                     PVHDVRAGALTLVRAAQYLPDGGIVLAVVDPGVGTDRRLVAVEVEHGILLGPDNGLLA
                     PAVAILGGAQRVVSLTSTEHQLPAPGPTFAGRDILAPAAALLVDGTEGPEALRAAVTS
                     PGGTTAAGLARLEAHGVRAAFLEAVEAATRRSEEIGRSDR"
     gene            complement(947..1141)
                     /locus_tag="VEP73_09040"
     CDS             complement(947..1141)
                     /locus_tag="VEP73_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26609.1"
                     /translation="MAESFAKARFLTVQEVAKLMRVSSMTVYRLIKAGDLPAVRVGRS
                     FRVRDVDVDAYLESRYTQAG"
     gene            complement(1264..>1714)
                     /locus_tag="VEP73_09045"
     CDS             complement(1264..>1714)
                     /locus_tag="VEP73_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HYT26610.1"
                     /translation="AALVRKGAAAIAGGRHATEAHLDEAERILGSVGVVVRVPEELLD
                     AVTGLSGSGPAYVFLVAEAMVEAGVLVGLPRETAATLVHQTLAGAAALLVDGTEGPEA
                     LRAAVTSPGGTTAAGLARLEAHGVRAAFLEAVEAATRRSEEIGRPDR"
BASE COUNT          240 a          618 c          634 g          222 t
ORIGIN      
        1 cagtcgcgcc tatccggcct gcgtgtaccg cgactccaga taggcgtcga cgtcgacgtc
       61 gcgcacccgg aacgagcgcc cgacgcgcac cgccggcagg tctcccgcct tgatcaggcg
      121 gtagaccgtc atggaggaca ccctcatcag cttggcgact tcctgcacag tcaggaagcg
      181 cgctttggcg aacgactccg ccacggtcaa caccccttgg ctccagcccg ctgggattct
      241 gagcgtaggt gggcggtgcc cctgttgtct agcgtgagcc gagcgcgccg gtgtcgaacc
      301 ctgtgggcac agggggccgg gaggccgtca gcggtcggag cgtccgatct cctcggagcg
      361 gcgggtcgcg gcctctaccg cttcgaggaa cgcggcccgg acgccgtggg cctcgaggcg
      421 ggccagcccg gcggcggtcg tcccgcccgg cgaggtcacc gcggcgcgga gcgcctccgg
      481 cccctcggtg ccgtcgacga gcagggcggc ggcgggggcg aggatgtcgc gtcccgcgaa
      541 cgtcgggccc ggcgcgggca gctggtgctc cgtgctggtg agcgagacca cgcgctgcgc
      601 gccgccgagg attgcgacgg ccggagcgag caggccgttg tcgggcccga gcaggatgcc
      661 gtgctcgacc tcgaccgcca cgagcctgcg gtcggtgccg acaccggggt cgaccaccgc
      721 gaggacgatg cccccatccg gcaggtactg cgccgcccgc accagggtga gggcgccggc
      781 gcgcacgtcg tggaccggca ggtcgtgggt gatgtcgatg atctggagat ccggggcgag
      841 gctcagcatg accgacttgc acaccccgac gaactcgtcg gagtggccgt agtcggacag
      901 gaacgagacg aaccgccggg cgagggtcac cctgtcccag tcgcgcctat ccggcctgcg
      961 tgtaccgcga ctccagatag gcgtcgacgt cgacgtcgcg cacccggaac gagcgcccga
     1021 cgcgaaccgc cggcaggtcc cccgccttga tcaggcggta gaccgtcatg gaggacaccc
     1081 tcatcagctt ggcgacttcc tgcactgtca ggaagcgcgc tttggcgaac gactccgcca
     1141 cggtccacac cccttggctc cagcccgctg ggaatctgag cgtaggtggg cggtgcccct
     1201 gttgtctagc gtgagccgag cgcgccggtg tccaagtctg tggacaacgg gacgcccggg
     1261 ccctcagcgg tcggggcgac cgatctcctc ggagcggcgg gtcgcggcct ccaccgcctc
     1321 gaggaaggcg gcccggacgc cgtgggcttc gaggcgagcc agcccggcgg ccgtcgtccc
     1381 ccccggcgag gtcaccgcgg cgcggagcgc ctcgggcccc tcggtgccgt cgacgagcag
     1441 ggcggcggca ccggccaggg tctggtgcac gagcgtggcc gcggtctcgc ggggcaaccc
     1501 gacgaggacg cccgcctcga ccatcgcctc cgcgaccagg aacacgtacg ccggacccga
     1561 gcccgagagg ccggtgacgg cgtcgagcag ctcctccggg acgcgcacga cgacgccgac
     1621 cgagccgagg atgcgctccg cttcgtcgag gtgcgcctcg gtggcgtggc gacctccggc
     1681 gatcgcggcg gcgccctttc ggacgagcgc ggcc
//
LOCUS       DASPYY010000570         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1360343, whole genome shotgun sequence.
ACCESSION   DASPYY010000570 DASPYY010000000
VERSION     DASPYY010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360343"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VEP73_09050"
     CDS             <1..498
                     /locus_tag="VEP73_09050"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HYT26611.1"
                     /translation="DVAATDVRARGVTASRVEVHLHGVSVARDVLLGRPGRVTIGRAD
                     GQVTLTEAEVDRLLASRLRGATIELSSEGVRLRVTADVLGQQVDGTVDGTLEARSAGI
                     AFVPSRISAPAGLDPSVQRELLSLFAVQVPLPPLPAGVQVERVVTEPHALVVSGRAGT
                     VQVAT"
     gene            946..1926
                     /locus_tag="VEP73_09055"
     CDS             946..1926
                     /locus_tag="VEP73_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HYT26612.1"
                     /translation="MDQPRLADALAAARAGVVAVERTDLATLALSGADVLRYLHTVST
                     QHTAGLAPGDAAEALLLSPKGKIEFAFRLAVLDPERLPGRERRGVLLDTQAEAAAALA
                     ERLARFVFRYDVAVGTPGAGALSLLGPEADAALAAAGLPVPPAAPGRAAVGGPALVAH
                     RTPAGVDVVGGAAPAALHALADAGVPAAPLAAWEALRVEHGLPRWGQELTDDVIAEEA
                     GLLGSHVHLDKGCYPGQETVARVHNLGQVQRRLAGLVFEGDELPPPRADLVTDDGRRA
                     GQVRTAVRHPDLGPVALAYVRRVVPAGRLVLAGPLVARVVDLPFTLARIA"
     gene            2069..2617
                     /locus_tag="VEP73_09060"
     CDS             2069..2617
                     /locus_tag="VEP73_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HYT26613.1"
                     /translation="MSVEVIPGGQRRIDRVLAPGFAAEAAALDLVELRARRDEAEAEE
                     ADISYLRRLVQGRLDILRAELARRSQGSDTDVAGLLAGLPAILSDQEPGTFNAVPRVR
                     TPSRADQHRRRVERLVSDETLARLPELAAGDLAAAVEVLTREEAVVSASRRAVQQVVD
                     ALRAELARRYRDGTAQVSQLLE"
     gene            2709..>3203
                     /locus_tag="VEP73_09065"
     CDS             2709..>3203
                     /locus_tag="VEP73_09065"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYT26614.1"
                     /translation="MQVLVEVERSGFVESRHFGTAVVADADGTVLAAVGDPELVVYPR
                     SCSKPFQALAVRGLGVQDELGLDEVALAGACGSHQGEPPHVASVRKVLAAAGLDETAL
                     RCPPVLPTDRAARATAPGPAAVYHNCSGKHAYMLAGTVVRGWEPERYTSPEHPLQAVV
                     TDTLV"
BASE COUNT          360 a         1237 c         1195 g          411 t
ORIGIN      
        1 gacgtggccg cgaccgacgt ccgcgccagg ggcgtcaccg cgtcccgcgt ggaggtccac
       61 ctccacggcg tgtccgtcgc ccgtgacgtg ctgctcggcc ggccagggcg ggtgaccatc
      121 gggcgggccg acggccaggt cacgctcacc gaggccgagg tcgaccggct gctcgcgagc
      181 cgcctgcgcg gcgccacgat cgagctctcg agcgagggcg tccggctccg ggtcacggcc
      241 gacgtgctcg gccagcaggt cgacggcacg gtggacggga cgctcgaggc gcgcagcgcc
      301 ggcatcgcct tcgtgccgag ccgcatctcc gcgccggccg gcctcgaccc gtcggtgcag
      361 cgggagctgc tttcgctgtt cgccgtccag gtgcccctgc ccccgctgcc ggccggcgtg
      421 caggtcgagc gggtcgttac cgagccgcac gcgctggtcg tttccggccg cgcgggcacg
      481 gttcaggtgg ccacctgata cgctcgcggc accgggcccg cgagagacac ggggcacggt
      541 tgcccaccac gacaggaacc gctcgcccat gaccctcgag ttctggtaca cggcgctgct
      601 gatcctcatg ctggccgcgg ccggggtgtt cgcggtctac gtcgtctacc gcctgctgaa
      661 ggactgaccg ccccgagccc gggcacgaaa agacccaggg ggtcggacgc ccgccttctg
      721 ggcggctccg cctcgactgg accaggtcga ggccccctgg gctcggtgat ctgtttggat
      781 tccgcggggc agatccgttc ccgcgattcg ggagcgtacc gccctaacgg gcagtcacct
      841 cacgggtccg taccggtcta tgcagtggga ccacctcctt tctcgcgtgg tgccagttag
      901 ccacagccgc cgcacgtgcg caacggagat cgctacgatg gggccatgga ccagccgcgc
      961 ctcgccgacg ccctggccgc cgcgcgcgcc ggcgtggtgg cggtcgagcg caccgacctc
     1021 gccaccctcg ccctcagcgg cgcggacgtg ctccgctacc tgcacacggt gtcgacccag
     1081 cacacggccg ggctggctcc cggcgacgcg gccgaggcgc tgctcctgtc cccgaagggc
     1141 aagatcgagt tcgccttccg gctcgccgtg ctcgacccgg agcgcctccc aggccgcgag
     1201 cggcgcggcg tcctgctcga cacgcaggcg gaggcggccg ccgcgctcgc cgagcggctg
     1261 gcgcggttcg tgttccgcta cgacgtcgcg gtggggacgc ccggcgccgg ggcgctcagc
     1321 ctgctcggac cggaggcgga cgccgccctg gccgcggccg ggctgccggt gccgccggca
     1381 gcgccgggcc gggcggccgt cggcggcccg gcgctggtcg cccaccgcac cccggccggt
     1441 gtcgacgtgg tcggcggggc ggcgccggcc gcgctgcacg cgctcgccga cgccggggtc
     1501 cccgccgcgc cgctcgcggc ctgggaggcg ctccgggtcg agcacggcct gccccgctgg
     1561 ggccaggagc tcaccgacga cgtgatcgcc gaggaggccg gcctgctcgg ctcgcacgtg
     1621 cacctggaca agggctgcta ccccggccag gagacggtcg ccagggtgca caacctgggc
     1681 caggtccagc gccggctggc cgggctcgtc ttcgagggcg acgagctgcc gccgccgcgg
     1741 gccgacctgg tcaccgacga cggccggcgg gccggccagg tgcgcaccgc cgtccgccat
     1801 cccgacctcg gcccggtcgc cctggcctac gtgcgccggg tggtcccggc cggacggctg
     1861 gtcctcgcgg ggccgctggt ggcgcgggtg gtcgacctcc cgttcacgct ggcccgcatc
     1921 gcctgaccgg acgggtcgcc ggtacggccg ggaaacgcac gtgcgccgcc accggtccgg
     1981 ctacaatgca cgttcgtgct gggagctggt tcccaggctg gacggacgcg gggtcggacg
     2041 ctcacggccc cggtccaagg gggggtccat gagcgtggaa gtcatccctg gcggtcagcg
     2101 ccgcatcgac cgcgtgctcg cacccgggtt cgccgcggag gccgccgccc tcgacctcgt
     2161 cgagctgcgc gcgcgccgcg acgaggccga ggccgaggaa gccgacatct cctacctgcg
     2221 ccggctcgtc cagggtcgcc tggacatcct gcgcgccgag ctggcgcgcc ggtcgcaggg
     2281 cagcgacacc gacgtcgccg gcctgctggc cggcctgccg gccatcctga gcgaccagga
     2341 gccgggcacc ttcaacgcgg tgccgcgcgt gcggaccccg tcccgcgccg accagcaccg
     2401 ccgacgggtc gagcgcctcg tctccgacga gacgctcgcc cgcctgcccg agcttgccgc
     2461 gggcgacctc gccgccgccg tcgaggtcct caccagggag gaggcggtcg tctcggccag
     2521 ccgccgggcg gtccagcagg tggtggacgc cctgcgggcc gagctcgccc gccgctaccg
     2581 ggacgggacc gcccaggtgt cgcagctgct cgagtgacca gggtgcccgg caggccgctg
     2641 gcggtcgcgc cgccgccgga ttagcctcgg aggctcctgg ctggccgtcg tccaagggag
     2701 ccgcgcgcat gcaggtcctc gtcgaggtgg agcgatcggg gttcgtcgag agccgccact
     2761 tcggcacggc cgtggtcgcc gacgccgacg gcaccgtgct cgccgcggtc ggcgacccgg
     2821 agctggtggt ctacccgagg tcgtgctcca agccgttcca ggcgctcgcc gtgcgcggcc
     2881 tcggcgtcca ggacgagctg ggcctcgacg aggtggcgct cgccggggcg tgcggctccc
     2941 accagggcga gccgccgcac gtcgcctccg tcaggaaggt gctggccgcc gccggcctgg
     3001 acgagaccgc cctgcggtgc cctcctgtgc tgccgaccga ccgtgcggcg agggcgaccg
     3061 ccccgggccc ggccgccgtg taccacaact gctctggcaa gcacgcctac atgctggcgg
     3121 gtaccgtcgt ccggggatgg gagccggagc gctacacctc gcccgagcac ccgctgcagg
     3181 ccgtcgtgac cgacacgctg gtc
//
LOCUS       DASPYY010000571         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1221878, whole genome shotgun sequence.
ACCESSION   DASPYY010000571 DASPYY010000000
VERSION     DASPYY010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221878"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /gene="cysS"
                     /locus_tag="VEP73_09070"
     CDS             complement(<1..292)
                     /gene="cysS"
                     /locus_tag="VEP73_09070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYT26615.1"
                     /translation="MLRLTNTLTRTREPVRPRTPGLLRMYSCGPTVYRYMHVGNLRSF
                     LVPDLVRRVLESEGVEVDHVQNVTDVGHMTDEELDRGEDKMLVSARLEDRTPA"
     gene            complement(352..579)
                     /locus_tag="VEP73_09075"
     CDS             complement(352..579)
                     /locus_tag="VEP73_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26616.1"
                     /translation="MRYRYEVRIGAADVGRRVVVRWRRPAAGGGDEVADVIGLLEAAD
                     GDAFSIRDRRGRLVVVPVARALAGKVVPPAR"
     gene            684..2270
                     /locus_tag="VEP73_09080"
     CDS             684..2270
                     /locus_tag="VEP73_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HYT26617.1"
                     /translation="MMPWSPHELVDRLTGRLAPLERELQEAYWAAATDARPETTAARA
                     RAEAAWLAALADRELFEEVEDGLARLSGAEPLLARALAEARRELLANQVPDDLRPELV
                     RLQAKVEAAFSTVRGQVGGHAVSNNEIDDVLARSGDPAERRAHWEAGKQVGAAVADDL
                     LALVELRNRIATGLGYRDWFAFSLATGDLDEAWLDATLGEVELATRAPFLAAKGALDQ
                     RLAARFGVAPDELRPWHYGDLFFQRADGEGEADLSPLLAGRDNVELAVAAYDGMGLET
                     RPVLARSDLHPRPGKDQHAFCLDVDRARDVRVLANVAPGEQWLDTMLHELGHAVYEDH
                     LASSLPWLLRRPPHALSTEAVALMLGRLRRDPEFLVGVAGADAGAARALADASREVLR
                     TGMLVFARWCLVMVRFEQAMYADPGRDLAAAWWDLVEELQGVHRPDGRTAPDWASKIH
                     LAVSPVYYQNYLLGELLASQLDAAVRERCGGFVGRPEAGAFLVDRVFSPGASFTWTGL
                     VESATGRRLTPEAFVAGLGR"
     gene            2442..2759
                     /gene="fdxA"
                     /locus_tag="VEP73_09085"
     CDS             2442..2759
                     /gene="fdxA"
                     /locus_tag="VEP73_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HYT26618.1"
                     /translation="MTYVISEPCIDVKDKSCIEECPVDCIYEGDRMLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDVPAQWKEFTTTNAAFFEELGSPGGAAKVGPQARDTDFVANYQ
                     PAE"
     gene            complement(2834..3976)
                     /locus_tag="VEP73_09090"
     CDS             complement(2834..3976)
                     /locus_tag="VEP73_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HYT26619.1"
                     /translation="MASADKTQWRELYHEVVETNLCTGCAACVMACPRDVLDYDHSET
                     YHPFNVELSTAVDDCVHGRRGCDICTRACPRFRAWELESDEALFGRAREAREIVGVQR
                     GVWLVRAAEPAVLEAGQDGGLVSALLIWGLASGRIEGALTSRRSTARVWDCEPFLATT
                     ESEVLEAAGSRYTYAANPLAMKEAERRGLKRLALVGMSCQASINGTLAARRLNKYARR
                     IELTIGLLCSKSFEYEGMKRIITEDYGIPLSDVAKVNIKGKFQVWRRSTGEEVDIPLK
                     KMQEATREGCRLCPDFAAEHADISAGGLGQTDGWTLTIVRTERGEEWLESARQAGVVT
                     VRPGSEDPAALALTVKLAARSRARWPVELAGTGAGAPGMLPIVQTT"
BASE COUNT          555 a         1650 c         1528 g          590 t
ORIGIN      
        1 ccgcgggcgt ccggtcctcg aggcgcgccg acacgagcat cttgtcctcg ccccggtcga
       61 gctcctcgtc ggtcatgtgc ccgacgtcgg tgacgttctg cacgtggtcg acctcgacgc
      121 cctccgactc gagcacgcgc cgcacgaggt cgggcacgag gaagctgcgg aggttcccga
      181 cgtgcatgta ccggtagacc gtcggcccgc acgagtacat ccgcaggagc ccgggggtcc
      241 gcggccgaac gggctccctg gtcctggtca aggtgttcgt caggcggagc atgcctggag
      301 gatgccggcc cggcgggccg acggcaaccg cgccgggcgg cccgggcggg gtcagcgcgc
      361 cggcgggacg accttgccgg ccagggcgcg cgccaccggg acgacgacga gcctgccgcg
      421 ccggtcgcgg atcgagaacg cgtcgccgtc ggccgcctcg agcaggccga tcacgtcggc
      481 cacctcgtcg ccgccgccgg cggcagggcg ccgccagcgc accaccaccc gccggccgac
      541 gtcagcggcg ccgatgcgga cctcgtaccg gtagcgcatg tgcgttccgc ggtcaggttg
      601 ccggtgcccc gtcggagtca ggttgccggt gccccgtcgg gcgcctctac agtagcgcca
      661 gcacacgtcc cccaggagac ctcatgatgc cgtggagccc gcacgaactc gtcgaccggc
      721 tcaccgggcg tctcgcgccg ctggagcggg agctgcagga ggcgtactgg gccgcggcca
      781 ccgacgcgcg acccgagacc accgccgccc gcgcccgcgc cgaggcggcc tggctcgccg
      841 cgctcgccga ccgcgagctg ttcgaggagg tcgaggacgg gctcgcgcgc ctgtccgggg
      901 ccgagccgct gctcgcccgc gccctcgccg aggcccggcg agagctgctc gccaaccagg
      961 tccccgacga cctgcggccc gagctggtcc gcctgcaggc caaggtggag gccgccttct
     1021 cgaccgtccg gggccaggtt ggcggccatg ccgtgtccaa caacgagatc gacgacgtgc
     1081 tcgcccggtc cggcgacccc gccgagcggc gggcccactg ggaggccggc aagcaggtcg
     1141 gggcggctgt cgccgacgac ctgctcgccc tcgtcgagct gcggaaccgc atcgcgacgg
     1201 gcctcgggta ccgcgactgg ttcgcgttct cgctggcaac cggcgacctg gacgaggcgt
     1261 ggctcgacgc gacgctcggc gaggtcgagc tggccacccg ggcaccgttc ctggccgcca
     1321 agggcgccct cgaccagcgc ctcgccgccc gcttcggggt ggcgccggac gagctccgtc
     1381 cctggcacta cggcgacctg ttcttccagc gtgccgacgg ggagggcgag gccgacctgt
     1441 ccccgctgct ggccggccgc gacaacgtcg agctggcggt cgccgcctac gacggcatgg
     1501 ggctcgagac gcgccccgtg ctcgcccggt ccgacctcca tccccgccca ggcaaggacc
     1561 agcacgcctt ctgcctcgac gtggaccgtg ccagggacgt gcgcgtgctc gccaacgtgg
     1621 cccccggcga gcagtggctc gacacgatgc tccacgagct cggccacgcg gtctacgagg
     1681 accacctcgc cagctcgctg ccgtggctcc tgcggcgccc gccccacgcc ctgtccaccg
     1741 aggcggtggc gctcatgctc ggccgcctgc gccgcgaccc ggagttcctc gtcggggtgg
     1801 ccggggccga cgcgggggcg gcccgcgccc tggccgacgc ctccagggag gtgctgcgca
     1861 ccggcatgct ggtgttcgcg cgctggtgcc tggtgatggt ccgcttcgag caggccatgt
     1921 acgccgaccc cggcagggac ctcgcggcgg cctggtggga ccttgtcgag gagctgcagg
     1981 gcgtgcaccg cccggacggc cgcacggcgc ccgactgggc cagcaagatc cacctcgccg
     2041 tgagccccgt gtactaccag aactacctcc tcggggagct gctcgcctcc cagctcgacg
     2101 cggccgtgcg cgagcgctgc ggcgggttcg tcggccggcc cgaggcgggc gcgttcctgg
     2161 tggacagggt cttctcgccg ggcgcctcgt tcacctggac gggcctcgtc gagtcggcca
     2221 ccgggcggcg gctcacgccc gaggcgttcg tcgccgggct cgggcggtga cccgtcccgc
     2281 ggccgggtct ccgggatggc cacaggggct gacctgccgc cacgaccccg acgcgcgtac
     2341 gatctgaacg gcgaccacgg gcggccccga ggtttgacgg tgccacgagc ccggtgatat
     2401 ccagcaggag cgtacgacgc tgacaagggg gttcacccag gatgacctac gtcatctccg
     2461 agccgtgcat cgatgtgaag gacaagtcct gcatcgagga gtgcccggtc gactgcatct
     2521 acgagggcga ccggatgctc tacatccacc ccgacgagtg cgtggactgc ggcgcatgcg
     2581 agccggtctg cccggtcgag gccatcttct acgaggacga cgtgccggcc cagtggaagg
     2641 agttcaccac caccaacgcc gcgttcttcg aggagctcgg ctctcccggc ggcgcggcca
     2701 aggtcggccc ccaggctcgc gacaccgact tcgtcgccaa ctaccagccc gccgagtagg
     2761 gcgcgggccg cgaggccccg cggccccggc ggccggccgg ggccgccgcc tccggagcgg
     2821 gtcctcgtgc gcgtcaggtc gtctggacga tcggcagcat cccgggagcg cccgcgccgg
     2881 tcccggccag ctccaccggc cagcgggccc tcgacctcgc ggccagcttg accgtcaggg
     2941 cgagcgccgc cgggtcctcg ctgcccgggc ggaccgtgac gacaccagcc tggcgggcgc
     3001 tctccagcca ctcctcgccc cgctcggtcc gcacgatggt gagcgtccag ccgtcggtct
     3061 ggccgaggcc gccagccgag atgtcggcgt gctcggcggc gaagtcgggg cagagcctgc
     3121 agccctccct ggtcgcctcc tgcatcttct tgagcgggat gtcgacctcc tcgccggtcg
     3181 agcgccgcca cacctggaac ttccccttga tgttgacctt cgcgacgtcg gagagcggga
     3241 tgccgtagtc ctcggtgatg atccgcttca tcccctcgta ctcgaacgac ttggagcaga
     3301 gcaggccgat cgtgagctcg atgcgcctgg cgtacttgtt gagccggcgg gccgccagcg
     3361 tgccgttgat cgacgcctgg cagctcatgc cgaccagcgc gaggcgcttg agcccccgcc
     3421 gctcggcctc cttcatcgcg agcgggttgg ccgcgtaggt gtagcgggag ccggccgcct
     3481 ccagcacctc gctctcggtg gtggccagga acggctcgca gtcccacacc cgggccgtcg
     3541 agcgtcggga ggtgagcgcc ccctcgatcc ggccgctggc gagcccccag atcagcagcg
     3601 ccgagacgag gccgccgtcc tggcccgcct cgagcaccgc gggctcggcg gccctgacga
     3661 gccagacccc gcgctgcacg ccgacgatct cgcgggcctc cctggccctc ccgaacagcg
     3721 cctcgtcgga ctcgagctcc cacgcccgga accggggaca cgcccgggtg cagatgtcgc
     3781 agccgcgccg cccgtgcacg cagtcgtcca ccgcggtcga cagctcgacg ttgaacgggt
     3841 ggtaggtctc gctgtggtcg tagtcgagca cgtcgcgcgg acaggccatc acgcaggctg
     3901 cgcagccggt gcacaggttc gtctccacca cctcgtggta gagctcacgc cactgggtct
     3961 tgtccgccga ggccatcgtc cctccggtcc cgcccccgca tggagcttcg ccgacaggct
     4021 agccaatgct gcggaaaccc gccggttgct tcaccatcgg aggggaagaa ggcacaggca
     4081 cccccgagcc cacaggccgc cacgtccctg gagccagatg aacccgttcc gaccgagtgc
     4141 cggcgaccgg ccgatggccg cccggacgac cctgatcgcg atcggggtgg cccttgccgt
     4201 ctcggcgctc ggcagcgtcg ccttcaccat ggccctgcac gacacgctgc tgtcggggct
     4261 ggtgacgttc gccatgttcg gcggcctggc cgcgctcacc gccggcgtgc tcgcctggga
     4321 ggt
//
LOCUS       DASPYY010000572         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2064131, whole genome shotgun sequence.
ACCESSION   DASPYY010000572 DASPYY010000000
VERSION     DASPYY010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064131"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VEP73_09095"
     CDS             <1..1587
                     /locus_tag="VEP73_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYT26620.1"
                     /translation="VAVVAASGGVNHALAYLLTEAGVGVSLAVGVGNAVDVTAADILS
                     WLAADEETRAVALHVESVPNGPALIAAVRELAARKPVVALVVGRNDVADFARSHTGAL
                     ATSWRTTRAALRHAGAVLVDDERQLTDAVTALSALRLPPTADPGVGVVTAQAGPGLLH
                     ADGLRGAGVAVPALAPATVSNLGGLLPPLTYQANPVDTGRPGETFAAVLRSVAADPAI
                     TLVSAYTLSEPDVLDLPAAVAAAEPGPLVVGLGGPSEEIRPQRDALRRLGVPVMTSPA
                     ALTTAVRALAEDARAQLMPRPGRRPASIPLVMKASPVDEDQAKTLLAALGVPTPPRRA
                     CRDRDEAHRALAELPGPVAVKILDAEVSHKSDIGGVHLGIRGRAGLDAALDALDRIGA
                     RRYLIETMAPPGVDLIVGASRDAVFGPVVLAGLGGTVAEALADVAVAPAPLAPAQAAS
                     LTSELAGRALLDGFRGGPVADRAAIGEVLAALGDLLAANPALEAVEINPLRVTGQGLL
                     ALDAVVTLAEPAASKETDHA"
     gene            1580..>1817
                     /locus_tag="VEP73_09100"
     CDS             1580..>1817
                     /locus_tag="VEP73_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxybenzoate-AMP ligase"
                     /protein_id="HYT26621.1"
                     /translation="MPDAPAVTPVAQGTAPWPEETVREYVARGWWRGRALGTELWSAA
                     DVRPGAPAVIDGAVRLTYRSVTARADALATKLTDD"
BASE COUNT          218 a          704 c          662 g          233 t
ORIGIN      
        1 gtcgccgtgg tcgcggccag cggcggggta aaccatgcgc tcgcctatct gctgaccgag
       61 gccggcgtcg gcgtgtccct cgcggtcggg gtcggcaacg cggtggacgt gacggcggcc
      121 gacatcctgt cctggctggc cgccgatgag gagacccgcg cggtggccct gcacgtcgaa
      181 tcggtaccga acggcccggc gctgattgcc gcggtcaggg aacttgccgc ccgcaagcct
      241 gtcgtcgcgc tcgtcgtcgg ccggaacgac gtcgccgact tcgcccggtc acacaccggc
      301 gcgctggcca cgtcgtggcg cacgacccgg gcggcgctcc ggcacgcggg cgccgtcctg
      361 gtcgacgacg agcggcagct caccgacgcc gtgaccgcgc tatcggcgct gcgcctgccg
      421 cccactgcgg accctggcgt cggcgtggtc accgcacagg ccgggccggg gctgctgcac
      481 gccgacggcc tgcgcggcgc aggggtcgcc gtgccggcgc tggcaccggc cacggtatct
      541 aacctcggcg ggctcctccc gccgctgacc taccaggcca acccggtcga tacaggccgg
      601 cctggcgaga ccttcgcggc ggtactacgc agcgtggccg ccgacccggc gatcacgctg
      661 gtgagcgcct acacgctcag cgagccggac gtcctcgacc ttccggccgc ggtggcggcc
      721 gcggaacccg gcccgcttgt cgtcggactc ggcggcccgt cggaggagat caggccgcag
      781 cgcgacgcgc tgcgccggct cggcgtaccc gtcatgacca gcccggccgc cctcaccacc
      841 gcggtccgcg cgctggccga ggatgcgcga gcccagctca tgccgcggcc tggccgcagg
      901 cccgcgagca tccccctggt catgaaggcc agcccggtgg atgaggacca ggccaagacg
      961 ctgctcgccg cgctgggtgt gcccaccccg ccccggcgtg cctgccgcga ccgcgacgag
     1021 gcgcaccgtg ccctggccga actgcccggt cccgtcgcgg tcaagatcct cgatgccgag
     1081 gtcagccaca agtccgacat cggcggggtc catctcggca tccgcggccg cgcggggctc
     1141 gacgcggccc tggacgcgct agaccgcatc ggggctcgcc ggtacctgat cgagacgatg
     1201 gcgccgcccg gcgtcgacct gatcgtcggc gcgtcgcggg acgcggtctt cggcccggtc
     1261 gtgctagccg gactgggcgg gacggtggcc gaagcgctgg ccgacgtcgc cgtcgcgccc
     1321 gccccgcttg cgcctgccca ggccgcgagc ctgaccagcg agctcgcagg ccgcgccctg
     1381 cttgacgggt tccgcggcgg tccggtcgcc gaccgcgccg cgatcggcga ggtcctggcc
     1441 gccctcggtg acctgctggc agctaacccg gctctggagg cggtcgagat caaccccctg
     1501 cgcgtcaccg gccagggcct gctcgccctc gacgctgtcg tgaccctcgc cgagccagcc
     1561 gcgtcgaagg agacggacca tgcctgatgc acctgctgtc accccggtgg cccagggaac
     1621 ggcgccctgg ccggaggaga ctgtccggga gtatgtggcc cgcggctggt ggcggggccg
     1681 tgcgctcggc accgaactgt ggagcgcggc tgacgtccgg cccggcgcgc ccgcggtaat
     1741 cgacggcgcg gtccggctga cgtaccggtc ggtgaccgcc cgggccgacg ctctcgccac
     1801 gaagctcacc gacgacc
//
LOCUS       DASPYY010000573         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2766173, whole genome shotgun sequence.
ACCESSION   DASPYY010000573 DASPYY010000000
VERSION     DASPYY010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766173"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..827)
                     /locus_tag="VEP73_09105"
     CDS             complement(93..827)
                     /locus_tag="VEP73_09105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYT26622.1"
                     /translation="MLYQVDNTHAEQARKAGQLGVAPGLARRTLDVLAASAGLLLLSP
                     VLLLLALAVRLSSPGPVLFRQTRVGQGFTRFTILKFRTMRAGSGGPEFTPAGDARITG
                     VGRLLRHSGLDELPQLLNVLWGDMTLVGPRPETPSLARRYPAECHAVFTYRPGLTGPS
                     QVRLRDKESLPANAVEDLERHYLGELVRKRAVLDLEYLSDPTVGRTLAVLVETFRYIV
                     RPVAAKLAGLRADARAGGAASRGARA"
     gene            complement(843..2312)
                     /locus_tag="VEP73_09110"
     CDS             complement(843..2312)
                     /locus_tag="VEP73_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HYT26623.1"
                     /translation="MTQTTSELVEDSTRRPADGLRCLVIGAGEAGRTIARDLARTPSF
                     GLVPVGFLDDDPGKHGATFAGLPVMGGTDEFVHAAEELAVDVVVVAIPSLPSREIRRL
                     GAEAAAVGASVRYLPSFVAAIERDARISDLRRLRVDRLLGRDEMRVVRTASRAVVAGK
                     RVLVTGAGGSIGSELCRQIRAFGPSALYLLDHDESNLHRLQLQISGEALLDSDELIIA
                     DIRDRSRILQLFRELQPEVVFHAAAHKHLPLLERHPCEGVKSNVLGTMHLAEAAVEAG
                     TERFILISTDKAADPTSVLGATKRLAELVVGSQAGGPTKFASVRFGNVLGSRGSFLTV
                     LAEQVENGEPITVTHPDVTRFFMTVEEAVGLVIEAGSMAASGEIFVLDMGEPVRIVDL
                     VHNYATQLNLDESDLTIRYTGLRPGEKLNEALFSKGEQRTPTAHPKVWSTVMRPPVGP
                     GFLAALDPLVAATEAGDPAEVRRRLSKLVPEYTPSPTRS"
     gene            complement(2309..3523)
                     /locus_tag="VEP73_09115"
     CDS             complement(2309..3523)
                     /locus_tag="VEP73_09115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYT26624.1"
                     /translation="MTALPDLSVETHRIPFARTDMGPDAVAAAARVLASGWVTTGPEV
                     VDFEREFAAHLGLRAGQAQAVSSCTAAIELALRALRLPAGAKVLTPTITFCGAVHAIV
                     NAGLAPVLVDCDAVTLAPDAATTAAAAARAGGVDAMVVLHYAGYPAPVEQLAAAAGLP
                     LGRVVEDAAHGLGTWVGDRPVGTISAATCFSFYATKNLPIGEGGMVTTTDPAIADFVR
                     RARLHGMSRDAWKRYLPGNGWRYAVETEGLKANMTDVQAAIGRVQLRRFDGWQARREE
                     LARRYEANLAGVAGLRPAARPAAGRHAWHLYVVQVEPEFGTPRDAVIEGLAARGVDCS
                     VHFIPLHHQPYFRELFGSDQAGRFPAADGAFERIVSLPFYPSLRDDQVDRVCEAIADL
                     RPAHTPRARTAL"
     gene            complement(3818..>4784)
                     /locus_tag="VEP73_09120"
     CDS             complement(3818..>4784)
                     /locus_tag="VEP73_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HYT26625.1"
                     /translation="EFDLFVDPSSTAGFTSDSNLFWNSTSVKPIKFNGTTYDTLAAYK
                     ATGHDAHSLSADPKFVDAPGGDYQLASGSPAVDSGTSKPAGYPTVDLNGAPRRDNAPT
                     PNTGQGPVTYYDMGAYERQADAVVAVPGALSMSTPRNPVTAGQPLVVTGTLANATTGA
                     KAAGLTVELWGHPYAKPFTRLRVGTTDATGRVTFSLLPPVNYRLYLRHPGSATLLPTS
                     SASAVGWVRAAVGARLSTSRMALGQTAVLSGTVRPNHAGQLVYLQRYLSGAWRSVASA
                     RLSTTSGYVFRIRPGVRSTLVLRVYKPADTSNLNGQSATLKLAVV"
BASE COUNT          626 a         1626 c         1900 g          632 t
ORIGIN      
        1 cggcgcccgc gcctgatccg cgcccgaccg tagcaggcta cgggcgaggc ggcatgccgc
       61 ctcgcccgta gcctgctacg gtcgggcgcg gatcaggcgc gggcgccgcg gctcgccgcg
      121 ccgccggccc gagcgtcggc acgcagcccg gcgagcttgg cggcgaccgg gcgcacgatg
      181 taccggaagg tctccaccag tacggcgagg gtacggccga cggtcgggtc cgacaggtac
      241 tcgaggtcga gcacggcccg cttgcggacc agctcgccga ggtagtggcg ctcgaggtcc
      301 tcgacggcgt tggcgggcag ggactccttg tcgcgcaggc ggacctgcga cggcccggtc
      361 agccctgggc ggtaggtgaa gaccgcgtgg cactccgcgg ggtagcggcg cgccagcgag
      421 ggcgtctcgg gcctcggccc gaccagggtc atgtcgcccc acagcacgtt gaggagctgg
      481 ggcagctcgt ccagcccgga gtgcctgagg agccggccga cgccggtgat gcgggcgtcg
      541 cccgccgggg tgaactccgg accgcccgag ccggcccgca tcgtgcggaa cttgaggatc
      601 gtgaagcgcg tgaagccctg gccgacccgg gtctgcctga acagcaccgg gcccgggctg
      661 gacagccgta ccgcgagcgc gagcagcagc agcaccgggc tgagcagcag gagcccggcc
      721 gacgcggcga gcacgtccag cgtgcgcctg gccagccctg gggcgacgcc cagctggcct
      781 gccttccgtg cctgctcggc atgggtgttg tcgacctggt agagcaaggg tacctccacg
      841 cgttaggacc gggtgggcga tggggtgtac tcggggacca gcttcgagag gcgacggcgc
      901 acctcggcgg ggtcgcccgc ctcggtggcc gcgacgaggg ggtcgagcgc ggcgaggaac
      961 ccgggtccga cgggcgggcg catcaccgtc gaccagacct tggggtgggc ggtcggggtg
     1021 cgctgctcgc ccttgctgaa cagcgcctcg ttgagcttct cgccagggcg cagcccggtg
     1081 tagcggatgg tgaggtcgga ctcgtcgagg ttgagctggg tcgcgtagtt gtggacgagg
     1141 tcgacgatgc ggaccggctc gcccatgtcg agcacgaaga tctccccgga ggcggccatg
     1201 ctccccgcct cgatgacgag gccgacggcc tcctcgacgg tcatgaagaa gcgggtgacg
     1261 tcggggtggg tgaccgtgat cggctcaccg ttctcgacct gctcggcgag cacggtgagg
     1321 aacgacccgc ggctgccgag cacgttgccg aagcgcacgg acgcgaactt cgtggggccg
     1381 cccgcctgcg acccgacgac cagctcggcg aggcgcttgg tggccccgag caccgaggtc
     1441 gggtcggccg ccttgtcggt cgagatgagg atgaaccgct cggtcccggc ctcgacggcc
     1501 gcctcggcca ggtgcatggt gccgagcacg ttggacttca cgccctcgca ggggtgccgc
     1561 tcgagcagcg gcaggtgctt gtgggctgcc gcgtggaaga cgacctcggg ctggagctcg
     1621 cggaacagct gcaggatccg gctccggtcg cggatgtcgg cgatgatcag ctcgtcggag
     1681 tcgagcagcg cctcgccgga gatctggagc tggagccggt gcaggttcga ctcgtcgtgg
     1741 tcgagcaggt acagggccga cggcccgaag gcgcggatct gacggcacag ctcgctgccg
     1801 atggagccgc cggcgccggt gacgagcacc cgcttgccgg ccacgaccgc gcgcgacgcg
     1861 gtgcggacga cccgcatctc gtcccggccg aggagccggt cgacgcgcag ccggcgcagg
     1921 tcggagatgc gcgcgtcccg ctcgatggcg gcgacgaacg acggcaggta gcggacgctc
     1981 gcgccgacgg cggcggcctc ggcgccgagg cgccggatct cgcgggacgg cagggagggg
     2041 atggccacga cgaccacgtc gacggccagc tcctcggcgg cgtggacgaa ctcgtcggtc
     2101 ccgcccatca ccggcaggcc ggcgaaggtg gcgccgtgct tgcccgggtc gtcgtcgagg
     2161 aacccgacag gcaccaggcc gaaagagggg gtgcgggcga ggtcgcgggc gatcgtccgc
     2221 ccggcctcgc cggcgccgat gacgaggcag cgaaggccgt cggccgggcg ccgggtcgag
     2281 tcctccacca gctcgctggt cgtctgggtc acagggccgt cctcgctcgt ggggtgtggg
     2341 cggggcggag gtcggcgatc gcctcgcaca ctcggtcgac ctggtcgtca cgcaggctgg
     2401 ggtagaaggg cagggacacg atccgctcga aggccccgtc cgcggcgggg aagcggccgg
     2461 cctggtcgct gccgaacagc tcgcggaagt agggctggtg gtgcagcggg atgaagtgga
     2521 ccgagcagtc gacgccgcgc gcggcgagcc cctcgatgac ggcgtcgcgg ggcgtcccga
     2581 actccggctc gacctggacg acgtacaggt gccaggcgtg cctgccggcc gcgggccggg
     2641 ccgccgggcg gaggccggcg acgccggcga ggttcgcctc gtagcggcgg gccagctcct
     2701 cccggcgtgc ctgccagccg tcgaaccggc gcagctgcac ccgcccgatg gcggcctgca
     2761 cgtcggtcat gttggccttg aggccctcgg tctcgacggc gtagcgccag ccgttgcccg
     2821 gcaggtagcg cttccaggcg tcgcggctca tgccgtgcag gcgcgcgcgc ctgacgaagt
     2881 cggcgatcgc ggggtcggtg gtcgtgacca tgccgccctc gccgatcggc aggttcttgg
     2941 tggcgtagaa gctgaagcag gtcgcggccg agatggtgcc gaccggccgg tcccccaccc
     3001 aggtcccgag cccgtgcgcg gcgtcctcga ccacccggcc gagcggcagc cccgccgcgg
     3061 cggcgagctg ctcgacgggg gccgggtagc cggcgtagtg gaggacgacc atcgcgtcga
     3121 cgccgcccgc gcgtgccgcg gcggcggccg tggtggccgc gtccggcgcg agcgtcacgg
     3181 cgtcgcagtc gaccaggacg ggcgcgaggc ccgcgttgac gatcgcgtgg accgccccgc
     3241 agaacgtgat ggtgggggtg agcaccttcg cgccggcggg caggcgaagg gcgcgcaggg
     3301 cgagctcgat cgcggccgtg cacgaggaga cggcctgcgc ctggccggcg cggaggccga
     3361 ggtgagcggc gaactcgcgc tcgaagtcga ccacctcggg cccggtggtg acccagccgg
     3421 aggcgaggac gcgcgccgcg gccgcgaccg cgtcgggccc catgtcggtc ctggcgaacg
     3481 ggatccggtg ggtctcgacc gagaggtcgg gcagggccgt catcggacgg cctcccgccc
     3541 ggcgcgccgg ccccgcgcgg aagcgtgcct tcgcgaccaa ctgcggtagt gcttactgcg
     3601 gtagtgcttc attcagcccc cctggcccgg atgtttgctt cggcttcacg tctcgttcat
     3661 gagcgacacc gcatagcgtg gccgacccag cacgtaaggg gatcgggcgg cggggggata
     3721 tcgactacag caaccacatg acccccgcga ccaacggcat ggccccgcgc ggagcgggcc
     3781 ccggccaccc aggccggggc ccgcgctcct gcctgcgcta gacgacggcg agcttgagcg
     3841 ttgcgctctg gccgttcagg ttgctcgtgt cggccggctt gtacacccgc aggaccaggg
     3901 tgctgcggac acccggcctg atgcggaaca cgtagccgct ggtcgtcgag agccgggccg
     3961 acgccacgct ccgccaggcg ccgctcagat agcgctggag gtagacgagc tggccggcgt
     4021 ggttcggccg gaccgtgccg ctgaggacgg cggtctgccc gagcgccatg cggctcgtcg
     4081 acagcctggc gccgaccgcc gcgcggaccc acccgacggc gctcgcgctg ctcgtgggca
     4141 ggagcgtggc gctccccggg tggcgcaggt agagccggta gttgacgggc gggagcaggc
     4201 tgaacgtgac ccgcccggtc gcgtcggtcg tgccgacgcg cagccgggtg aagggcttgg
     4261 cgtacgggtg gccccacagc tcgacggtca gcccggccgc cttcgcgccg gtcgtcgcgt
     4321 tggcgagcgt cccggtcacc accagcggct gcccggcggt gaccgggttg cgcggcgtgc
     4381 tcatcgagag cgcgcccggc acggcgacga ccgcgtcggc ctgccgctcg taggcgccca
     4441 tgtcgtagta cgtgaccggg ccctggccgg tgttgggcgt cggcgcgttg tcgcggcgcg
     4501 gggcgccgtt caggtcgacg gtcgggtagc cggcgggctt gctcgtgccg gagtcgaccg
     4561 cgggcgagcc gctcgcgagc tggtagtcgc cgcccggcgc gtcgacgaac ttcgggtcgg
     4621 ccgagagcga gtgggcgtcg tgcccggtcg ccttgtacgc ggcgagggtg tcgtaggtgg
     4681 tgccgttgaa cttgatcggc ttcacgctgg tcgagttcca gaagaggttg gagtcggagg
     4741 tgaagccggc ggtggagctg gggtcgacga acaggtcgaa ctcg
//
LOCUS       DASPYY010000574         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2344762, whole genome shotgun sequence.
ACCESSION   DASPYY010000574 DASPYY010000000
VERSION     DASPYY010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344762"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VEP73_09125"
     CDS             <1..618
                     /locus_tag="VEP73_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYT26626.1"
                     /translation="SFYNESAGRRIHPTPVVGVLGVLDDAAGVVRSGFPRPGLEVHLL
                     GETRVELGGSAWQRLATGGRLEGRPPALDLAAEARLHRLLVDLAAGRLLASAHDLSDG
                     GLAVALVEAVLGEPSVGATIALPAPPAPLAALVSESASRVLVSVDPAAAPAVTAAASA
                     AGVPIRRLGRTGGTALVVPGVLDLPIDRLREAYEGAIPRALGEGS"
     gene            complement(794..1462)
                     /locus_tag="VEP73_09130"
     CDS             complement(794..1462)
                     /locus_tag="VEP73_09130"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HYT26627.1"
                     /translation="MARHLPHARLHARRVARLGAVLALSAAVVVSVAALPAAAGTIHV
                     VQRGESLASIAARGKLDSWRPIWDANAGLTDPNLIYPGQRLAVPAKGEKVEHRPLPAG
                     FGVVAQPAAVARPSTARTEPARVRPARVRSSVAGRVRSSVAGGTVWDRLARCESGGNW
                     STSTGNGFYGGLQFSASTWRAAGGTGSASQASRSEQIRVAQRVLARQGWGAWPACSSQ
                     LGLR"
     gene            1791..>2231
                     /locus_tag="VEP73_09135"
     CDS             1791..>2231
                     /locus_tag="VEP73_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26628.1"
                     /translation="MDRKTGLARFRLRGLARFREVGGMDRVTAGWRLATAILAVAGTL
                     APAPATAPPAPRASNTALVGHLDPGPGPYGDVWVHAGTAYLGSFRSRACATAPGGLTA
                     VDVRDPARPRAVGRFAQFRGSDDEDVWVGHVRTSSFVGDLAATGV"
BASE COUNT          263 a          857 c          826 g          285 t
ORIGIN      
        1 agcttctaca acgagtcggc cggccgccgc atccacccga ccccggtcgt cggcgtgctg
       61 ggcgtgctcg acgacgcggc cggcgtggtg cggtcggggt tcccgcgccc gggcctcgag
      121 gtccacctgc ttggcgagac ccgtgtcgag ctgggcggct cggcctggca gcggctggcc
      181 accggcggcc gcctcgaagg ccgcccgccc gcgctcgacc tggccgcgga ggcccggctg
      241 caccggctgc tcgtggacct ggccgccggc cggctgctcg cgagcgcgca cgacctgagc
      301 gacggggggc tcgccgtcgc gctggtggag gcggtgctcg gagagccgag cgtcggcgcg
      361 accatcgcgc tgcccgcgcc gccggcaccg ctcgccgccc tggtcagcga gtccgcctcc
      421 cgggtgctgg tcagcgtcga ccccgccgcc gcgccggccg tgaccgccgc cgcctccgcg
      481 gccggcgtcc cgatccgccg cctcggccgg accgggggca ccgccctggt cgtccccggg
      541 gtcctcgacc tgccgatcga ccggctgcgc gaggcgtacg agggcgccat cccccgcgcc
      601 ctcggcgagg ggtcctgatc ccgcccctgc cacgtcccgg gaacgacgac acggcgcgcg
      661 gtccgaagac cgcgcgccgt gtccgttacc ccacgagggt gcctgcatgc gggcactcga
      721 gcgcgcacgc aggcgtcagg tcgcggtgtc gttcaacagg tcgtcgttca gtgtgtcgtc
      781 cggacggcgg gagctaccgc aggccgagct ggctgctgca ggcaggccag gcaccccagc
      841 cctgccgggc gagcacgcgc tgtgccaccc ggatctgctc gctccggctc gcctggctgg
      901 ccgagccggt gcctccggcc gcccgccagg tcgacgccga gaactgcaga ccaccgtaga
      961 agccgttgcc ggtgctggtg ctccagttcc cgccggactc gcacctggcg agccggtccc
     1021 agaccgtgcc gccggccacc gaggaccgca cgcgaccggc caccgaggac cggacgcggg
     1081 ccgggcgaac acgcgccggc tcggtacggg ccgtgctcgg ccttgcgacc gcggccggct
     1141 gggcgaccac accaaacccc gctgggagcg ggcggtgctc caccttctcg cccttcgcgg
     1201 ggacggccag ccgctggccg ggatagatga ggttcgggtc ggtcaggccg gcgttggcgt
     1261 cccagatggg ccgccaggag tcgagcttgc cccgcgccgc gatgctggcg aggctctcgc
     1321 cacgctggac gacgtggatg gttccagccg ctgccggcag cgctgccacc gacaccacga
     1381 ctgccgcgct gagcgcgagg accgcgccca ggcgggccac ccggcgtgcg tgcagccggg
     1441 catgcggaag atgacgtgcc aaaggaaacc tccaggcttc acatgttgtg tgcgtaggcc
     1501 gcagcaggcc ctggggtggg accggctcgc gtgtgcgcca tcacgcggcg cacctcgaca
     1561 cgccgccacg cggagcgtcg acgcccctca gagggggcgg ccgccgtcag ggggcggctg
     1621 ccggaggagc gccgtggggt ccgcgactgc ctccagccat acgagccgag gacggtagac
     1681 gcgcatcgag caaagatcaa aggatgtcca ctacggctcg agacgttcgt ggtgttccgt
     1741 ccggagtgcc tggtcgcttt cttagctgcg cctcaggctt ggttgtcacc atggaccgca
     1801 agacgggcct cgcccgcttc aggcttcggg gcctcgcccg cttcagggag gtgggtggca
     1861 tggaccgcgt gacggccgga tggcggttgg cgacggccat cctggcggtc gcggggaccc
     1921 tcgcaccggc gcccgccacc gccccgccgg ccccccgcgc ctccaacacc gcgctggtcg
     1981 gccacctcga cccgggcccg ggcccgtacg gcgacgtctg ggtccatgcc ggcaccgcct
     2041 acctcggcag cttccggagc agggcgtgcg ccaccgcgcc tggcgggctg acagccgtcg
     2101 acgtgcggga cccggcccgg ccgcgcgccg tcggccggtt cgcccagttc cggggcagcg
     2161 acgacgagga cgtgtgggtc ggccacgtgc gcaccagctc gttcgtcggc gacctggccg
     2221 ccaccggggt g
//
LOCUS       DASPYY010000575         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2485827, whole genome shotgun sequence.
ACCESSION   DASPYY010000575 DASPYY010000000
VERSION     DASPYY010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485827"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VEP73_09140"
     CDS             <1..969
                     /locus_tag="VEP73_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT26629.1"
                     /translation="SICAPNMLHREIGVAAARAGKPFWIEKPVGRDAGETAEVEAAAR
                     AAGVTTSIGYNYRHVPAVERVRELIAAGRLGRVTNVRAVFFNSYAAEPNGALSWRFLR
                     EKAGTGALGDLLSHVVDLMQYVVAPITEVSSLLSTVYAQRPILPMGSGTHFAVIENGE
                     MGDVENDDYVAALVRFAASSHAAGAVGTLEASRVSVGPQCSLGFEIYGTDGSAVWNFE
                     QMNELRLCLGRGGADHGYTTVLGNAALGDYGRFQPGPGTSMSYDDLKVIEARKFLVAA
                     GGGEQCNSTIAEAHADAEVIAATAASARDGAWHKVAPVPGATFGHA"
     gene            969..>1592
                     /locus_tag="VEP73_09145"
     CDS             969..>1592
                     /locus_tag="VEP73_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26630.1"
                     /translation="MPSHVRAGDLPVVVALGSVDPDLVSGVLHGHCRFVATPGDAELA
                     VAEGAIARADARVDRALLGRAPALRVIARTGVGTDLIDVPAATARGVAVVITPGVGAA
                     AVAEGAIGMALHLVKRFGPLTEMVRDGRWAERAGVSVGDLDGATLGVVGYGRIGRRTA
                     ALASAFGMRVLAYDPVSEPPANLPSVEYCASLGDLAARSDVITLHLPL"
BASE COUNT          207 a          578 c          593 g          214 t
ORIGIN      
        1 tccatctgcg cgccgaacat gctgcaccgc gaaatcggcg tcgcggccgc ccgggcgggc
       61 aagccgttct ggatcgagaa gccggtcggc cgggacgccg gcgagaccgc cgaggtcgag
      121 gccgcggccc gggcggccgg cgtcaccacc tcgatcggct acaactaccg gcacgtcccc
      181 gcggtggaac gggtccgcga gctgatcgcg gccggccggc tcggccgcgt caccaacgtg
      241 cgcgcggtgt tcttcaacag ctacgcggcc gagcccaatg gcgccctgtc ctggaggttc
      301 cttcgcgaga aggccggaac cggcgcgctc ggcgatctgc tcagccacgt cgtcgacctg
      361 atgcagtacg tggtggcgcc gatcaccgag gtcagctcgc tgctgtcgac ggtgtacgcg
      421 cagcggccca tcctgccgat gggatccggc acgcatttcg ccgtgatcga gaacggcgag
      481 atgggcgacg tcgagaacga cgactacgtc gcggcgctgg tccggttcgc ggccagttcc
      541 cacgcggccg gcgccgtcgg caccctggag gcgtcccggg tcagcgtcgg tccgcagtgc
      601 tcgctcggct tcgagatcta cggcacggac ggctcggccg tctggaactt tgagcagatg
      661 aacgagctgc ggctctgcct gggccgaggc ggagccgacc atggttacac caccgtgctc
      721 ggcaacgccg ctctgggcga ctacggccgc ttccagccgg gcccaggcac cagcatgagc
      781 tacgacgacc tgaaggtcat cgaggccagg aagttcctgg tcgcggcggg cggcggcgag
      841 cagtgcaact cgaccatcgc cgaagcgcac gccgacgccg aggtcatcgc cgcgacagcg
      901 gcctcggccc gggacggcgc ctggcacaag gtggcgccgg tccccggagc cacgttcggg
      961 cacgcctgat gcccagccat gtgcgggccg gcgacctgcc ggtcgtggtg gcgctgggct
     1021 ccgtcgaccc ggacctggtg tccggcgtgc tccacgggca ctgccggttc gtcgcgacgc
     1081 ccggcgatgc cgaactcgcc gtcgccgagg gcgcgatcgc ccgggccgat gcccgggtcg
     1141 accgggcgct gctgggccgg gcgcccgcgc tccgggtcat cgccaggacc ggggtcggca
     1201 ccgacctgat cgacgtgccg gccgcgacgg cccggggggt cgccgtggtg atcaccccgg
     1261 gggtcggcgc cgccgcggtc gccgagggcg cgatcggcat ggcgctgcac ctggtcaagc
     1321 ggttcggccc gctgaccgag atggtccgcg acgggcgctg ggccgagcgc gccggcgtca
     1381 gcgtcgggga cctcgacggc gccacgctgg gcgtggtcgg ctacggccgg atcggccgcc
     1441 ggaccgcggc gctggcctcg gccttcggga tgcgggtcct ggcctacgat ccggtgagcg
     1501 agccgccggc caacctgccc agcgtggagt actgcgccag cctcggcgac ctcgcggcgc
     1561 gcagcgacgt catcaccctg caccttccgc tg
//
LOCUS       DASPYY010000576         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1362052, whole genome shotgun sequence.
ACCESSION   DASPYY010000576 DASPYY010000000
VERSION     DASPYY010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362052"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VEP73_09150"
     CDS             <1..195
                     /locus_tag="VEP73_09150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HYT26631.1"
                     /translation="HRSVEPGASAALAELELDPLVDLGMRLGEGTGALLAVPIVRAAA
                     AALARMATFAEVGIGTGASS"
     gene            282..1640
                     /locus_tag="VEP73_09155"
     CDS             282..1640
                     /locus_tag="VEP73_09155"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HYT26632.1"
                     /translation="MATTSPDPDAPAVRFPPGFLLGAATAAYQIEGAVRADGRGLSIW
                     DTFSHTPGRIADGSTGDVACDHYHRWREDVALLADLGLNAYRFAVAWPRVLPEGTGRV
                     NQAGLDFYRRLVDALLEAGVTPALTLYHWDLPQALQDRGGWAERSTADAFAAYAEVVA
                     GALGDRVPLWITHNEPSVVTLAGHVEGKHAPGVSDLRTAVQVAHHLLLSHGLAAQALR
                     AAGAGEVGITLNLYAVEPAGDAEADVAAAGRAHAVVNRWFLEPVAHGRYPAELLAWFE
                     EEGLMPAARDGDLDTIRQPLDFLGVNYYTRLVAAAGPGAVLGYRDVGQAGERTEMGWE
                     VYPEGLHQVLSGLAREGVAPALYVTENGAAFADQADPDGRVRDPRRVAYLDAHFRAAA
                     RAIAEGVPLRGYFVWSLLDNFEWAFGYTKRFGLAYVDYPTQRRIVKDSGRYVREVARG
                     EP"
     gene            complement(1643..>2014)
                     /locus_tag="VEP73_09160"
     CDS             complement(1643..>2014)
                     /locus_tag="VEP73_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26633.1"
                     /translation="NVATQRRDPDSLLSLYRRLLWYRRGSGALRWGGYHPLPAAPDGL
                     FAFLRETAAERLLVALNFTDRPACLRSAEAPTGRLELSTGTAGAGREVGLQPLQVDAN
                     EGLVVRLSPPGEVGRFAHAVP"
BASE COUNT          242 a          793 c          729 g          250 t
ORIGIN      
        1 caccgctcgg tcgagccagg cgcgtccgcg gcgctcgccg agctcgagct ggacccgctc
       61 gtcgacctcg gcatgcgcct cggcgagggg accggcgcgc tgctcgcggt cccgatcgtc
      121 cgtgcggccg ccgccgccct cgcccggatg gcgacgttcg cggaggtcgg catcggcacc
      181 ggcgcgtcga gctgagcggg acgccgaggt gcctggaagc gtcccctccc gcgtgaccag
      241 gggacctacg gctgccgccg ccgatccggt aggctcgggc catggcgacc acctcacccg
      301 acccggacgc gccggccgtc cggttcccgc ccgggttcct gctcggcgcc gccaccgcgg
      361 cctaccagat cgagggggcg gtccgtgccg acggccgtgg cctgtcgatc tgggacacgt
      421 tcagccacac gccgggacgg atcgccgacg gctcgaccgg cgacgtcgcc tgcgaccact
      481 accaccgctg gcgcgaggac gtcgccctgc tcgccgacct cggcctgaac gcctaccggt
      541 tcgccgtcgc ctggccccgg gtcctgcccg aggggaccgg ccgggtcaac caggccggcc
      601 tggacttcta ccgcaggctc gtcgacgccc tgctcgaggc gggcgtcacc ccggccctca
      661 ccctctacca ctgggacctt ccccaggccc tgcaggaccg gggcggctgg gccgagcggt
      721 cgacggccga cgcgttcgcc gcctacgccg aggtggtcgc gggcgcgctc ggcgaccggg
      781 tgcccctgtg gatcacccac aacgagccgt ccgtcgtcac cctcgccggg cacgtcgagg
      841 gcaagcacgc gcccggggtc agcgatctcc ggaccgccgt ccaggtcgcc caccacctgc
      901 tgctctccca cggcctggcg gcgcaggcgc tgcgggcggc cggcgccggc gaggtcggca
      961 tcacgctgaa cctgtacgcg gtcgagccgg ccggcgacgc cgaggccgac gtcgccgccg
     1021 ccggccgcgc ccacgcggtg gtcaaccgct ggttcctcga gccggtcgcg catggccgct
     1081 accccgccga gctgctggcg tggttcgagg aggagggcct catgccggcg gcgcgggacg
     1141 gcgacctcga caccatccgg cagcccctcg acttcctcgg cgtcaactac tacacgcgcc
     1201 tggtcgccgc ggccggcccg ggcgccgtgc tcggctaccg tgacgtcggg caggcggggg
     1261 agcgcaccga gatgggctgg gaggtctacc cggagggcct ccaccaggtc ctgtccgggc
     1321 tggcccgcga gggcgtggca cccgccctgt acgtcaccga gaacggtgcc gccttcgctg
     1381 accaggccga cccggacggg cgggtgcgcg acccgcgccg cgtcgcctac ctggacgcgc
     1441 acttccgggc tgcggcgcgg gcgatcgccg agggcgtgcc gctgcgcggc tacttcgtgt
     1501 ggagcctgct ggacaacttc gagtgggcct tcggctacac caagcggttc ggcctcgcct
     1561 acgtcgacta cccgacccag cggcgcatcg tgaaggactc cggccgctac gtccgcgagg
     1621 tcgcccgcgg cgagccgtag cgtcacggca ccgcgtgggc gaaccggccc acctcgcccg
     1681 gcggggagag ccgcacgacc agcccctcgt tcgcgtcgac ctggagcggt tgcaggccca
     1741 cctcccggcc ggcaccggcg gtgccggtcg acagctcgag ccgccccgtc ggcgcttcgg
     1801 cgcttcgcag gcacgccggg cggtcggtga agttcagcgc caccagcagc cgctcggcgg
     1861 cggtctcccg gaggaacgcg aacagcccgt cgggagccgc cggcagcgga tggtagccgc
     1921 cccagcgcag cgcgcccgaa cctcgccggt accacagcag cctacggtac aggctgagca
     1981 gggagtcggg atcgcggcgc tgggtggcca cgtt
//
LOCUS       DASPYY010000577         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_799961, whole genome shotgun sequence.
ACCESSION   DASPYY010000577 DASPYY010000000
VERSION     DASPYY010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799961"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="rph"
                     /locus_tag="VEP73_09165"
     CDS             complement(<1..621)
                     /gene="rph"
                     /locus_tag="VEP73_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYT26634.1"
                     /translation="MTRRDGRAPGALRPVAITLGWQATAYASALVEVGRTKVVCAVSH
                     EDRTPPFLRGTGRGWVTAEYSMLPGATSERSPREVAKGRAGGRTHEIQRLVGRSLRAV
                     TDTSAFPESTLWVDCDVLQADGGTRCAAITGAWVALFAACHRLVQDKRARTFALRDHV
                     AAVSAGIVEGVPCLDLPYEEDARAETDMNVVMTGLGGLVEVQGTAER"
     gene            complement(618..1406)
                     /locus_tag="VEP73_09170"
     CDS             complement(618..1406)
                     /locus_tag="VEP73_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT26635.1"
                     /translation="MIRLTVLGSCGTYPAPGRACSGYLLEAAPDRDGGRSVRVWVDTG
                     GGTLSNLLRYVPPTELDAIWVSHLHVDHCSDLPLAYYALRYGDYEPRAARLPVYGPAG
                     WAGHLGEFMSRGTGSTLEDVFEVCELADGERLAVGPLELTAVATVHSLETYGVRAAVE
                     GRTLAYSADSGPCEGLGRLADGADLFLCEAAWTERPPGADPHHLTPAEAGRYAASACA
                     RRLVLTHLRPHEDPEAALGLAGEAYGGDVDVAVEGNVYQLGGTP"
     gene            complement(1403..>1864)
                     /locus_tag="VEP73_09175"
     CDS             complement(1403..>1864)
                     /locus_tag="VEP73_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYT26636.1"
                     /translation="EATHENVTVTSQACPGLVEFVERGETTGQPVLDAAGAYLRPLTE
                     ADVDTLILGCTHYPLLTGVIGYLMGPDVFLVNSAEWTARNVFAELTRADLHAPYGAAA
                     RHRFLASGDPGEFRRLGARFLGPEVNRVERRAAAPVPGWVAAAQPAEAWAP"
BASE COUNT          234 a          666 c          746 g          218 t
ORIGIN      
        1 ccgctcggcg gtgccctgga cctccacgag accgccgagg ccggtcatga cgacgttcat
       61 gtcggtctcg gcacgcgcgt cctcctcgta cggcaggtcg aggcagggca cgccctcgac
      121 gatgcccgcg ctgaccgcgg ccacgtggtc gcgcagcgcg aaggtcctcg cccgcttgtc
      181 ctgcacgagg cggtggcagg cggcgaacag cgccacccag gcgcccgtga tggcggcgca
      241 gcgggtcccg ccgtcggcct gcagcacgtc gcagtcgacc caaagggtcg actccgggaa
      301 cgccgaggtg tcggtgacgg cccgcaggga gcggccgacg aggcgctgga tctcgtgggt
      361 gcggccgccc gcgcggccct tggccacctc ccggggggaa cgttcgctgg tcgcccctgg
      421 cagcatcgag tactcggcgg tgacccagcc cctgccggtc ccgcgcagga acggcggggt
      481 acggtcctcg tggctgaccg cgcagacgac cttggtccgg ccgacctcga ccagggccga
      541 ggcataggcg gtcgcctgcc agccgagcgt aatggcgacc ggccgcagcg cgccgggcgc
      601 gcggccgtct cttcgggtca cggcgttcct ccgagctggt agacgttccc ttcgacggcg
      661 acgtccacgt cgccgccgta cgcctcgccg gcgaggccga gggcggcctc ggggtcctcg
      721 tgcgggcgca ggtgggtgag cacgagccgt cgggcgcacg ccgaggcggc gtaccggccg
      781 gcctcggccg gggtcaggtg gtgcgggtcg gcgccgggcg gccgctcggt ccaggccgcc
      841 tcgcagagga acaggtcggc accgtcggcc agccgcccga gaccctcgca cggccccgag
      901 tcggccgagt acgcgagcgt gcggccctcc acggccgcgc ggaccccgta ggtctcgagg
      961 ctgtggaccg tggcgaccgc ggtgagctcg agcgggccga ccgccaggcg ctcgccgtcg
     1021 gcgagctcgc acacctcgaa cacgtcctcg agcgtggacc cggtgcccct cgacatgaac
     1081 tcgccgaggt ggccggccca gccggccggg ccgtagacgg ggagccgggc cgcgcgcggc
     1141 tcgtagtcgc cgtagcggag ggcgtagtag gcgaggggca ggtcgctgca gtggtcgacg
     1201 tggagatggc tgacccagat cgcgtcgagc tcggtgggcg ggacgtagcg gagcaggttc
     1261 gacagcgtgc ccccgccggt gtcgacccac acccgcacgg accggccgcc gtcgcggtcg
     1321 ggcgccgcct cgagcaggta gccggagcag gcccgcccgg gggccgggta ggtgccgcag
     1381 gagcccagga cggtgagccg gatcacggcg cccacgcctc ggccggctgc gcggcagcga
     1441 cccagccggg cacgggggcc gcggcgcgcc gctcgacccg gttgacctcc ggcccgagga
     1501 agcgggcgcc gaggcggcgg aactcgcccg ggtccccgga ggcgaggaag cggtggcggg
     1561 ccgccgcccc gtagggggcg tggaggtcgg cgcgggtgag ctccgcgaag acgttgcggg
     1621 cggtccactc cgccgagttg acgaggaaga cgtccggccc catgaggtag ccgatcaccc
     1681 cggtcagcag ggggtagtgg gtgcagccga ggatcagcgt gtcgacgtcg gcctcggtca
     1741 gggggcggag gtaggccccg gcggcgtcga ggaccggctg gccggtggtc tccccgcgct
     1801 ccacgaactc gacgagcccg gggcaggcct gggaggtgac ggtcacgttc tcgtgggtcg
     1861 cctc
//
LOCUS       DASPYY010000578         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1925290, whole genome shotgun sequence.
ACCESSION   DASPYY010000578 DASPYY010000000
VERSION     DASPYY010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925290"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..2042)
                     /locus_tag="VEP73_09180"
     CDS             complement(141..2042)
                     /locus_tag="VEP73_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26637.1"
                     /translation="MMGYRYMRAADIEPGKRLSRGVVRRVWGFAGAYRLQIVLYLSLI
                     GLSALVAVVPPLLVRTLINDAIPRRSFRLVDLIGLAAVGIALATAGLALAQRFLSARI
                     GEGLIYDLRTALFDHVQRQPLAFFTHTQTGALVSRMNNDVVGAQQALTSTLGSVTSNV
                     IGLVATLSAMFLLNWRITLVALVVLPLFLLPAKRVGKRLQAITRESMNLNASMNSVMN
                     ERFNVAGALLVMLFGRRGRELAEFSGKAARVRDIGVRSAMFGQTFFVALGLVGAVGTA
                     AVYWLGARLVLDGRLRAGDVVALAAYVTQVYGPLTSLTNARVAVMTAFVSFERVFEVL
                     DHESPIADRPGARDLADPVRGEIEFDHVSFRYPSAEDSSIASLTGGAAAVGASPGDLV
                     LRDVSFSARPGELVALVGPSGAGKTTTAMLVPRVYDVDAGVVRVDGVDVRDVTLESLR
                     AAIGLVAQDPHLFHDTILANLRYAKPDATMEEVAAACRAARIADLIEGLPEGYDTLVG
                     DRGYRLSGGEKQRLAIARLLLADPAIVILDEATAHLDSESEVHIQEALAEALSGRTSL
                     VIAHRLSTIVRADRILVVDGGQVVEQGTHAELLAGEGLYAGLYATQFGRAPAVSPGAA
                     LPSSVPASA"
     gene            2288..>2847
                     /locus_tag="VEP73_09185"
     CDS             2288..>2847
                     /locus_tag="VEP73_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26638.1"
                     /translation="MRRRLLAHFGLALGLALGLALVPGLAWAHHPDVSGSEQCDGTLT
                     WTVSAWRDGSPAARSNPEVQVKVDGTVVQVGAFTRDNGFQFGGTVALGAPRTVVVGVK
                     TLAKWGDGYWAGQERQTTVHAPGDCQTPPSSTAPPMTTSPPSSTSPSSSTAPSSSGTS
                     TTVAPTTSSAGPTATLPAPPTTSVVPT"
BASE COUNT          405 a         1026 c         1039 g          377 t
ORIGIN      
        1 cgggacggtt gggggtcgac gtcctggagc tccgggccgc gccgccgccc tcgaggccgg
       61 tcttctccgg cccggaggac gccggcggcg ggtgacccgc cgcgcgccgt gcggcctggg
      121 cccccgcgga gcttctccgg ctaggcgctc gccgggaccg agctcggcag ggcggcgccg
      181 ggagagacgg cgggtgcccg gccgaactgc gtggcgtaga ggccggcgta gagaccctcg
      241 ccggcgagca gctccgcgtg cgtgccctgc tccacgacct ggccgccgtc caccaccagg
      301 atgcggtcgg cccgcacgat cgtggagagc cggtgggcga tcaccaggct ggtgcgaccg
      361 gagagcgcct cggccagcgc ctcctggatg tggacctcgg actcggagtc gaggtgggcg
      421 gtcgcctcgt ccaggatcac gatcgcgggg tcggcgagca gcagccgggc gatcgccagg
      481 cgctgcttct ccccgcccga cagccggtag ccgcggtcgc cgaccagcgt gtcgtacccc
      541 tccgggaggc cctcgatcag gtcggcgatg cgggcggccc ggcacgccgc cgcgacctcc
      601 tccatcgtcg cgtcgggctt cgcgtagcgg aggttcgcga ggatggtgtc gtggaagagg
      661 tgggggtcct gggcgaccag gccgatggct gcgcgcaggc tctcgagggt gacgtcgcgg
      721 acgtcgaccc cgtccacccg gaccacgccg gcatccacgt cgtagacccg cgggaccagc
      781 atggcggtgg tcgtcttgcc ggcgcccgac ggcccgacca gtgcgaccag ctcgcccggg
      841 cgggcgctga acgacacgtc acgcagcacg aggtcgcccg gggacgcacc gaccgcggcg
      901 gccccgccgg tgaggctcgc gatcgaggag tcctcggcgc tcgggtacct gaagctgacg
      961 tggtcgaact cgatctcgcc ccgcaccggg tcggcgaggt cgcgggcccc aggccggtcg
     1021 gcgatcgggc tctcgtggtc gagcacctcg aacacccgct cgaaggacac gaaggcggtc
     1081 atcacggcga cacgggcgtt ggtgagggac gtgagcgggc cgtagacctg ggtgacgtag
     1141 gcggccaggg cgaccacgtc cccggcccgg aggcggccgt cgagcacgag ccgggccccg
     1201 agccagtaca cggccgcggt gccgaccgcg ccgaccaggc cgagggccac gaagaaggtc
     1261 tggccgaaca tggccgaccg caccccgatg tcgcgcaccc gggccgcctt gcccgagaac
     1321 tcggccagct cccgccccct gcgcccgaac agcatcacga gcagcgcgcc cgccacgttg
     1381 aagcgctcgt tcatcaccga gttcatcgag gcgttgaggt tcatcgactc gcgcgtgatg
     1441 gcctggagcc gcttgccgac gcgcttcgcg gggaggagga acagaggcag gacgacgagc
     1501 gcgaccagcg tgatccgcca gttcagcaag aacatggcgg acagcgtcgc gaccaggccg
     1561 atcacgttcg aggtgaccga gccgagcgtg ctggtcagcg cctgctgcgc gcccacgacg
     1621 tcgttgttca tccggctcac gagcgcgccg gtctgggtgt gggtgaagaa cgccagcggc
     1681 tggcgctgga cgtggtcgaa cagggcggtg cgcaggtcgt agatgagccc ctcgccgatc
     1741 cgggcggaga ggaagcgctg ggccagggcg agcccggcgg tggccagggc gatccccacc
     1801 gccgccaggc cgatgaggtc gacgagccgg aagctcctgc gcgggatggc gtcgttgatg
     1861 agcgtccgga ccagcagcgg cgggaccacg gcgacgagcg ccgacagccc gatcaggctg
     1921 aggtacagga cgatctggag gcggtaggcg ccggcgaagc cccagacgcg tcgcacgacg
     1981 ccgcgcgaga gccgcttgcc tggctcgatg tcggcggccc tcatgtagcg gtagcccatc
     2041 atctcgctcc gagcaaccgc gcccggcggc cgttcattcc acggcggccc ggcccgccgc
     2101 ccggccactc acccgtcgtc ttggggatac ttgcgtgctt catctggttc gaggctgttt
     2161 atcattccga tattcacctg tgtcgcgagg ttcacctggt acgccgggag cgtccggttc
     2221 atgagctaga gtccgggctc gtcctttgaa ggtctcgcct cggtccggtc cgtgaggagt
     2281 tcgttcgatg cggagaagac tgctggcaca ctttggcctg gccctcggcc tggccctcgg
     2341 cctggcgctc gttcccggcc tcgcatgggc ccaccatccc gacgtctccg gcagcgagca
     2401 gtgcgacggc accctcacct ggacggtgag cgcgtggcgg gacggcagcc ccgccgcgag
     2461 gtccaatccc gaggtccagg tgaaggtcga cgggaccgtc gtccaggtcg gggcattcac
     2521 gcgggacaac gggttccagt tcggcggcac cgtggcgctg ggcgccccca ggaccgtcgt
     2581 cgtcggcgtc aagaccctcg ccaagtgggg cgacggctac tgggccggtc aggagcgcca
     2641 gacgaccgtc cacgcgccag gcgactgcca gacgccgccg tccagcaccg cgccgccgat
     2701 gaccacctcg ccgccctcca gcacgtcccc gtcgagctcc acggcaccga gctcgtccgg
     2761 cacctcgacc accgtggcgc ccaccacctc gtcggccggc ccgacggcca cgctgccggc
     2821 gccgccgacc acctcggtgg tcccgac
//
LOCUS       DASPYY010000579         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2766899, whole genome shotgun sequence.
ACCESSION   DASPYY010000579 DASPYY010000000
VERSION     DASPYY010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766899"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEP73_09190"
     CDS             complement(<1..222)
                     /locus_tag="VEP73_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYT26639.1"
                     /translation="MQLPASVSIREVGPRDGLQTERPVPVAGRVRLVDALSATGVRRI
                     EVGSFVSPRSVPAMAGTGQVFAAIERRPGV"
     gene            complement(302..952)
                     /locus_tag="VEP73_09195"
     CDS             complement(302..952)
                     /locus_tag="VEP73_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYT26640.1"
                     /translation="MPFPDRLLGENEQVVYDLRPHWWALVGPALLAVLIVVVTSLAWG
                     LMPGGSLQGGARVAVQVAGLAALAVWVLPRVVGWATTHFVLTTDRLIFRRGFVSRFAR
                     EIPLERVNDVTFSQSLFERLIGAGDLLIESAGEHGQSAFENIRDPEGVQLEIYRRMEE
                     NGRRMLAQQVTPPHPRAFGGPSSPLDDLERLAALHERGLLSDEEFARKKRDLLDRM"
     gene            1244..>1760
                     /locus_tag="VEP73_09200"
     CDS             1244..>1760
                     /locus_tag="VEP73_09200"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HYT26641.1"
                     /translation="MRRLIPSLAGLAVVALAAAGCTGQSRPAPTRGAASQLSGSASGA
                     ATSPSLTATPAGRTPMTVYYPRDTGDAWYLVAERHQVPSDVSRVVAAVTELLTVGPRY
                     AGSAPPFPAGSRLLGADVRGTTATVNLSREVMRVPRGEGAEYAVQALVWTVTRAERHV
                     RRVLVEVEGRTS"
BASE COUNT          235 a          639 c          677 g          209 t
ORIGIN      
        1 gaccccgggc cggcgctcga tggccgcgaa gacctggccg gtgcctgcca tcgcgggcac
       61 cgacctcggg gagacgaacg acccgacctc gatccggcgg acgcccgtgg ccgacagggc
      121 gtcgaccagc cggacccgcc cggccacggg caccggccgc tcggtctgga gcccgtcgcg
      181 ggggcccacc tcgcggatgc tgaccgacgc tgggagctgc aaccgccacc tcggcaggac
      241 gtggttgacg cgacgatcgt acccgcgacc ctgggccggc ggcgcggagc cgggcggcgg
      301 gctacatgcg gtcgaggagg tcgcgcttct tgcgcgcgaa ctcctcgtcg gacagcaggc
      361 cgcgctcgtg cagggcggcg agccgctcga ggtcgtcgag ggggctcgac ggcccgccga
      421 acgcccgcgg gtgcggcggc gtgacctgct gggccagcat ccgccggccg ttctcctcca
      481 tgcggcggta gatctcgagc tgcacgccct ccgggtcgcg gatgttctcg aaggccgact
      541 ggccgtgctc gcccgccgac tcgatgagca ggtcgccggc gccgatcagc cgctcgaaga
      601 gcgactgcga gaacgtgacg tcgttgacgc gctcgagcgg gatctcgcgg gcgaacctgg
      661 acacgaagcc gcggcggaag atcagccggt cggtcgtcag cacgaagtgg gtggtcgccc
      721 agcccacgac ccgcgggagg acccagaccg cgagggcggc caggcccgcg acctgcacgg
      781 cgacgcgggc gcccccctgg aggctcccgc caggcatgag gccccaggcg aggctggtga
      841 cgacgacgat gaggacggcc aggagcgcgg gaccgacgag cgcccaccag tgcgggcgga
      901 ggtcgtagac gacctgctcg ttctcaccaa gcagccggtc ggggaacggc acgcttcgcg
      961 acctcctcgg aaccggcgcc ctcgggaccg gcgcccggac ggcacggagc gtagcaacgg
     1021 cctcgtctcg tctcaaggaa cctcggggaa ccgttcgatc tcgtgcaatc ggttacagtg
     1081 cgggcgcgcg cccaggatca gcgggcgggc aggcgcggtg cggttaccgt tcggggccag
     1141 atcggggccg tcccgttgcc gattcgcaac catgtgggct gaccgtgggg agcccatgcc
     1201 gggccgtcat ggctcgcgcg gcacgcagga ggccagggac ggcatgcgac ggctgattcc
     1261 gagtctggcg ggtctcgcgg tggtggcgct cgccgccgcc gggtgcaccg ggcagagccg
     1321 gccggcgccg acgcgcggcg cggcctcgca gctctccggt tcggcgtccg gggcggcgac
     1381 cagcccgagc ctcacggcca cgccggccgg caggacgccg atgaccgtct actacccgcg
     1441 ggacaccggc gacgcctggt acctcgtcgc ggaacggcac caggtcccgt cggacgtgtc
     1501 gcgggtcgtg gccgcggtca ccgagctgct caccgtcggc ccgcgctacg cgggctcggc
     1561 cccgccgttc ccggccggct cgcggctgct cggggccgac gtccgcggga cgaccgccac
     1621 ggtcaacctc tcccgcgagg tcatgcgcgt gccccgcggc gagggcgccg agtacgcggt
     1681 gcaggccctt gtctggaccg tgacccgggc cgagcggcac gtgcgacggg tcctggtcga
     1741 ggtcgagggg cggacctcgg
//
LOCUS       DASPYY010000580         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_96494, whole genome shotgun sequence.
ACCESSION   DASPYY010000580 DASPYY010000000
VERSION     DASPYY010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96494"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VEP73_09205"
     CDS             complement(<1..739)
                     /locus_tag="VEP73_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT26642.1"
                     /translation="MQPNARSRDVIGLVSRPNPKRPLLNRRLEGLGTTIFAEMSARAV
                     ATGSINLGQGFPDRDGPAEGARAAADAVLEGRGNQYPPGPGIPELRQAIAAHDERFYG
                     LRYDPDGEILVTAGATEAIAAAMLALVEPGDEVIAFEPYYDSYAANIAMAGGVRVPVT
                     LRPPGFRPDLHELAKAVTDKTRLILLNSPHNPTGSVFTRDELAAIAALAVERDLLVVT
                     DEVYEHLVFDGEHIPLATLPGMAERTLT"
     gene            743..1567
                     /locus_tag="VEP73_09210"
     CDS             743..1567
                     /locus_tag="VEP73_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYT26643.1"
                     /translation="MRVALCQIPVSSRPEVNSGRVRDALAEAAGLGADLAVFPEATQA
                     RFGSDLRAAAEPLNGPFCSGLATAAKQAGVALVAGVFEPAPEGRVYNTAVAYDGTGEL
                     VASYRKLHLFDAFGHRESDEVAPGSVPVLCTLGGVRVGLAICYDVRFGELSRALAVAG
                     AELLALPAAWAAGLFKEEHWVTLVRARAIENTMWVAAVDQVPDPDERPTRAATGIGRS
                     MLVDPLGVVRADLGPAPGVAVAEVDTGLIATVRATLPSLANRRDDVFGPPAPVREA"
BASE COUNT          257 a          686 c          778 g          303 t
ORIGIN      
        1 tcgtcagcgt ccgctcggcc atgccgggga gcgtcgccag cgggatgtgc tcgccgtcga
       61 agacgaggtg ctcgtagacc tcgtcggtga ccacgagcag gtcgcgctcg acggccagcg
      121 ccgcgatggc ggcgagctcg tcgcgggtga agaccgagcc ggtcgggttg tgcgggctgt
      181 tgagcaggat gagcctggtc ttgtcggtga ccgccttggc cagctcgtgc aggtcggggc
      241 ggaagcccgg cgggcgcagc gtgaccggca cgcggacgcc gcccgccatc gcgatgttgg
      301 ccgcgtagga gtcgtagtag ggctcgaacg cgatcacctc gtcgcccggc tcgaccaggg
      361 cgagcatggc ggccgcgatc gcctcggtgg cgcccgcggt gaccaggatc tcgccgtccg
      421 ggtcatagcg caggccgtag aaccgctcgt cgtgcgcggc gatggcctgg cgcagctcgg
      481 gtatgcccgg gccgggcggg tactggttgc cacggccctc gaggacggcg tcggccgccg
      541 cccgggcgcc ctcggccggc ccgtcccggt cggggaagcc ctggcccagg ttgatggacc
      601 cggtcgcgac cgcgcgggcg gacatctcgg cgaatatcgt cgtcccgagg ccctcgaggc
      661 gccggttcag cagtggtcgt ttcggattgg gtcggctcac gagcccaatg acatcacgtg
      721 atcgcgcgtt aggctgcatg gtatgcgtgt cgcgttgtgc cagatcccgg tcagctcacg
      781 acccgaggtc aactccgggc gggtcaggga cgccctggcc gaggccgccg gcctgggcgc
      841 cgacctggcg gtgttccccg aggcgaccca ggccaggttc ggctcggacc tgcgcgccgc
      901 ggccgagccc ctgaacggcc cgttctgctc cgggctggcc accgcggcca agcaggctgg
      961 ggtcgcgctg gtggcaggcg tcttcgagcc cgcgccggag ggccgggtct acaacaccgc
     1021 cgtggcctac gacgggacgg gcgagctggt cgcctcctac cgcaagctgc acctgttcga
     1081 cgcgttcggg caccgcgagt ccgacgaggt cgcgcctggg tccgtcccgg tcctctgcac
     1141 gctcggcggg gtgcgcgtgg gcctggcgat ctgctacgac gtgcggttcg gcgagctgtc
     1201 ccgggcgctg gccgtggccg gggccgagct gctggcgctg cccgcggcgt gggccgccgg
     1261 gctgtttaag gaggagcact gggtcacgct ggtgcgggcc cgcgccatcg agaacaccat
     1321 gtgggtcgcc gcggtggacc aggtgcccga cccggacgag cggcccaccc gggccgcgac
     1381 cggcatcggg cgcagcatgc tggtggaccc gctcggcgtg gtccgggccg acctcgggcc
     1441 agcgcccggc gtggccgtcg ccgaggtcga caccggcctg atcgccacgg ttcgagccac
     1501 cctgcccagc ctggccaacc gccgcgacga cgtcttcggc ccgcccgccc ccgtccgcga
     1561 ggcgtagcag caggctggct cctgcccgcg aggcatgacc gcaggctcgc tcctgcccgc
     1621 gagccctgag cccgcaggcc gacgggtgag gtggcggctg tgcggttgcc agcggcaggg
     1681 cgccagggag attatcggcg acagtggccg tgacgtcggc gtacacatgt gactcacgat
     1741 gcctgattgc cccgtgttgc gctgcacctg caaaagatcg tggaagaaat ggcttatgcg
     1801 gaggatccgg tacctcctgg gggtccattt cctccactta gcccgaatcc tccgcgttaa
     1861 tttccgacct atcgaataaa tatacagaat ttgtgacagg tggggtcacc gtggccagtg
     1921 aggtggctag ctcgggcggt cgggacggca cggggtgccg gggcgggaag cgccggggca
     1981 tggggccgcc ggcacggggg cgcgggggtg ccggggcacg ggcg
//
LOCUS       DASPYY010000581         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_940875, whole genome shotgun sequence.
ACCESSION   DASPYY010000581 DASPYY010000000
VERSION     DASPYY010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940875"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VEP73_09215"
     CDS             complement(<1..669)
                     /locus_tag="VEP73_09215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HYT26644.1"
                     /translation="MVSQFFAAPADLTDEVAVLRGGEARHAAQVLRLKVGERLRVADG
                     AGRVAEATALAVDRHEVRAQVSAVRVAPPRRPAVTVVAGLVKGGRLDLVVQKLTELGV
                     ERIAPAVCRRSVVDWDARKRTAARDRWAAIALAAAKQSHRDRVPVVAPVDHLEAHVAA
                     APGPRMVCWEGGGRPLSAALPADAPDALTLVVGPEGGLEAAEVAACTGAGGVPVTLGP
                     IVLRA"
     gene            complement(698..1831)
                     /gene="dnaJ"
                     /locus_tag="VEP73_09220"
     CDS             complement(698..1831)
                     /gene="dnaJ"
                     /locus_tag="VEP73_09220"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYT26645.1"
                     /translation="MTDYYAVLGVGRDATSDEIKRAYRKLARQYHPDVNGGDTAAEER
                     IKEVNRAYSVLSDPEKRQRYDTFGEAGIDGQGQGPSVADPFGFGFGSFDDVVREFFGG
                     GDPFVGRGRRRSRSRRGEDIEERLSIDFVEAVFGTSREIQYVAAAVCPRCHSSGAEPG
                     TEPTVCRSCRGSGQQRTVRQSLLGQLVTTTTCSVCGGSGQEVLTPCAKCKGHGRVAEK
                     VKLPVEIPAGVEGGMQIRYHGRGHAGEFGGPPGDLYVRLTVAPHKVFERDGDNLLCRM
                     PVPMTVAAIGGSVTLVTLDGEEDIEVEPGTQSGTVRRFRKRGVPHTDGRGRGDLLVEL
                     VVQTPDDLSEEERRLLRQLAELRGEAVNPQPAGFFARIKGSGR"
     gene            complement(1886..2911)
                     /gene="hrcA"
                     /locus_tag="VEP73_09225"
     CDS             complement(1886..2911)
                     /gene="hrcA"
                     /locus_tag="VEP73_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYT26646.1"
                     /translation="MNLDDRKARLLRAVVHDFIQTAEPVGSKTLAERYALGVSAATIR
                     NELASLEEQGYLSHPHTSAGRVPTDRGYRFYVDSLSGVGKLARAQEEAIVRYFDGTAD
                     LEETLRRTSTLLAGLTSYTAMVAPPSLDRSRLRHVEVVGIGRHVILLVLIMDTGRVEK
                     RLVEAAGEVGQGDLEDLRRQLNEALGGERLDRAELILSAMAEDVPPDRRVLFATLANA
                     IGQVVGDQSSGRVWVDGQAHIAGPGGFGGLEAVHQVYQALEQQVLVLQLLQAALGQDR
                     VSVIIGAENPVQGMEACSFVTSSYLAGDATGSIGVLGPTRMDYLRAMAAVQAVARYLG
                     DVLDDVG"
     gene            complement(3001..4143)
                     /gene="hemW"
                     /locus_tag="VEP73_09230"
     CDS             complement(3001..4143)
                     /gene="hemW"
                     /locus_tag="VEP73_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HYT26647.1"
                     /translation="MPFCLTRCHYCDFVTYTGMEGLRRPYAAALLREAEVAAAGLGPE
                     PPTVTSVFVGGGTPTLLPPGDLALVLDRLRGLLPFAPGAEVTVEANPETVDPAVAEGL
                     AAAGATRVSMGAQSFDDRVLGALGRPHGAAQTGRAVAALRAAGIEAVNLDLIFGCPHE
                     DAASWAATLAAAVGLAPEHVSAYALTIEPATKFGRLVAGGRMAAPDEDLLADRYEATC
                     AALAATGYRHYEVSNWARDDRAPRPGAGIARLPARASRHNLTYWRRGAYLGLGAGAHE
                     FTGGVRRWNVPAVPEYLAAVTAGRRPTGGEERLSAAEAAFELLALRLRTADGLDPAEA
                     ARLGVDPEGPAVAELRAAGLLAAGGRVALTEKGMFLHGEVVSRLSG"
     gene            complement(4178..>4769)
                     /locus_tag="VEP73_09235"
     CDS             complement(4178..>4769)
                     /locus_tag="VEP73_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216920.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HYT26648.1"
                     /translation="VVRATVISPAEYIGTIMELCQSRRGELQGMDYLSAERVELRYTL
                     PLAEIIFDFFDALKSRTRGYASLDYEPSGYREASLVKVDLLLQGEPVDAFSAIVHKDK
                     AYAYGRAMATRLKQLIPRQLYEVVVQAAIGSRVIARETISARRKDVLAKCYGGDITRK
                     RKLLEKQKEGKRKMKQIGRVEVPQEAFVAALRTDAD"
BASE COUNT          597 a         1804 c         1750 g          618 t
ORIGIN      
        1 cgcgcgcagg acgatcggcc cgagggtgac cggcaccccg ccggccccgg tgcacgcggc
       61 cacctcggcc gcctcgaggc cgccctccgg ccccacgacg agcgtcagcg cgtcgggggc
      121 gtcggcgggc agggccgccg acagcggccg cccgcctccc tcccagcaga ccatgcgggg
      181 accgggcgcc gcggccacgt gcgcctcgag gtggtccacg ggcgcgacca ccggcacccg
      241 gtcgcggtgg ctctgcttgg cggcggcgag cgcgatcgcc gcccagcggt cgcgggcggc
      301 cgtgcgcttg cgcgcgtccc agtcgaccac cgagcggcgg cagaccgccg gggcgatgcg
      361 ctccaccccg agctcggtca gcttctgcac caccaggtcg agcctgccgc ccttgacgag
      421 cccggcgacg acggtgacgg ccgggcgcct cggcggcgcc acgcgcaccg cggagacctg
      481 ggcgcgcacc tcgtggcggt ccacggccag ggccgtggcc tcggccaccc gcccggcgcc
      541 gtcggccacc cgcagccgct cgccgacctt gaggcggagc acctgcgcgg cgtggcgcgc
      601 ctcgccgccc cgcaggaccg cgacctcgtc ggtcaggtcc gccggggcgg cgaagaactg
      661 gctcaccatc ggcgcgggcg tggtggggcg gccgggttca gcggccggag cccttgatgc
      721 gggcgaagaa cccggccggc tgggggttga ccgcctcgcc gcgcagctcg gcgagctggc
      781 gcaggaggcg ccgctcctcc tcgctgaggt cgtccggcgt ctgcacgacc agctcgacca
      841 gcaggtcgcc acggccccgg ccgtcggtgt gggggacgcc gcgcttgcgg aagcgcctga
      901 ccgtgcccga ctgggtgccc ggctcgacct cgatgtcctc ctccccgtcc agggtgacga
      961 gggtcaccga gccgccgatg gcggcgacgg tcatggggac gggcatgcgg cacagcaggt
     1021 tgtcgccgtc gcgctcgaac accttgtggg gcgcgaccgt gagccggacg tagaggtccc
     1081 cgggcggccc gccgaactcc ccggcgtgcc cgcggccgtg gtagcggatc tgcatcccgc
     1141 cctcgacccc ggcggggatc tcgaccggca gcttcacctt ctcggctacc cgcccgtgcc
     1201 ccttgcactt ggcgcacggg gtcagcacct cctgcccgct gcccccgcag accgagcagg
     1261 tggtggtggt gacgagctgg cccagcaggg actggcgcac ggtccgctgc tggccgctgc
     1321 cgcggcagga gcggcagacg gtcggctccg tgcccggctc ggcgccgctc gagtggcagc
     1381 gggggcagac ggccgccgcg acgtactgga tctcccggga cgtgccgaag acggcctcga
     1441 cgaagtcgat cgacagccgc tcctcgatgt cctcgccccg gcgggagcgg gagcggcgcc
     1501 ggccgcggcc gacgaacggg tcgccgccgc cgaagaactc cctgaccacg tcgtcgaagc
     1561 tcccgaaccc gaacccgaac gggtcggcca cggacggccc ctggccctgg ccgtcgatgc
     1621 cggcctcgcc gaaggtgtcg tagcgctgcc gcttctcggg gtcgctgagc acgctgtagg
     1681 cccggttgac ctccttgatg cgctcctcgg cggccgtgtc gccgccgttc acgtccgggt
     1741 ggtactggcg cgcgagcttc cggtaggcac gcttgatctc gtcggacgtg gcatcgcggc
     1801 cgacgccgag cacggcgtag tagtcggtca cccgtctccc ctcgacctcg caagcctcat
     1861 ccggaacgat cgcacggctc gtgcctcacc cgacgtcgtc gagcacgtcg ccaaggtacc
     1921 tggcgacggc ctggaccgcc gccatggcac gcagatagtc cattctcgtc ggtccgagca
     1981 cgccgatgct gcccgtcgcg tccccggcca ggtagctcga ggtgacgaac gagcaggcct
     2041 ccatgccctg caccgggttc tccgcgccga tgatgaccga cacacggtcc tggccgagcg
     2101 cggcctggag caactgcagg acgagcacct gctgctcgag cgcctggtag acctggtgca
     2161 cggcctcgag gccgccgaac ccgcccggcc cggcgatgtg ggcctggccg tcgacccaga
     2221 cccgcccgct gctctggtcg ccgaccacct ggccgatcgc gttggccagg gtggcgaaca
     2281 ggacccggcg gtccgggggg acgtcctcgg ccatggccga caggatcagc tcggcccggt
     2341 ccagccgctc gccgccgagc gcctcgttga gctggcggcg caggtcctcg aggtcaccct
     2401 ggccgacctc gccggcggcc tcgaccaggc gcttctccac gcggcccgtg tccatgatga
     2461 gcacgagcag gatgacgtgg cgtccgatgc cgaccacctc gacgtgccgc aggcgggagc
     2521 ggtcgagcga cggcggcgcc accatggccg tgtagctggt caggccggcg agcagcgtcg
     2581 aggtccggcg cagggtctcc tccaggtcgg cggtgccgtc gaagtagcgg acgatcgcct
     2641 cctcctgggc acgcgccagc ttgccgaccc ccgacagcga gtcgacgtag aagcggtagc
     2701 cgcggtcggt gggcacccgg cccgcgctgg tgtgggggtg gctcaggtag ccctgctcct
     2761 cgaggctggc gagctcgttg cggatggtcg cagcggacac ccccagcgca tagcgctcgg
     2821 ccagggtctt ggaccccacc ggctcggcgg tctggatgaa atcgtgcacg acggcgcgca
     2881 acaggcgcgc ctttcggtca tcgaggttca tggccgtcct tcctgcacca aggcagcgta
     2941 gcactctgca tagtcgagtg ctgaacgccc ccgcccccgc gacggggtgg gggccccgcc
     3001 ctaccccgag aggcggctca cgacctcgcc atgcaggaac atccccttct cggtgagggc
     3061 cacccgcccg ccggcggcga gcagccccgc cgcccggagc tcggcgacgg ccgggccctc
     3121 cgggtcgacg cccagccgcg ccgcctcggc cgggtcgagg ccgtcggccg tgcgcagccg
     3181 cagggcgagc agctcgaacg ccgcctccgc ggccgacagg cgctcctcgc cgccggtggg
     3241 gcggcggccg gcggtcaccg cggccaggta ctccggcacg gcgggtacgt tccagcgccg
     3301 gacgccgccg gtgaactcgt gcgcgccggc gccgagcccg aggtaggcgc cgcgccgcca
     3361 gtaggtgagg ttgtgccggc tggcccgggc gggcaggcgc gcaatgcccg ccccggggcg
     3421 cggggcgcgg tcgtcgcgcg cccagttcga cacctcgtag tggcggtagc cggtggcggc
     3481 cagggcggcg caggtggcct cgtagcggtc ggccagcagg tcctcgtcgg gggcggccat
     3541 gcggccgccg gcgaccagcc gcccgaactt ggtggccggc tcgatggtga gcgcgtacgc
     3601 ggacacgtgc tcgggggcga gcccgaccgc tgcggcgagg gtggcggccc aggacgccgc
     3661 gtcctcgtgg gggcagccga agatcaggtc gaggttgacc gcctcgatcc cggcggcgcg
     3721 cagcgccgcc accgcccggc cggtctgggc cgcgccgtgc gggcggccga gggcgccgag
     3781 cacccggtcg tcgaagctct gggcgcccat cgaaacccgg gtcgcccccg cggcggcgag
     3841 tccctcggcc accgccgggt cgaccgtctc cgggttggcc tcgacggtca cctcggcgcc
     3901 gggcgcgaac gggaggagcc cgcgcagccg gtcgagcacc agggcgaggt cgcccggggg
     3961 cagcagggtc ggcgtcccgc cgccgacgaa cacgctcgtc accgtcggcg gctcgggccc
     4021 gaggcccgcc gccgcgacct ccgcctcgcg cagcagggcg gccgcgtacg ggcgccgcag
     4081 cccctccatg ccggtgtagg tgacgaagtc gcagtagtgg caccgggtca ggcagaacgg
     4141 cacgtgcacg tacaccccga agaggggggg tgccgtgcta gtccgcatcc gtccgcaggg
     4201 cggccacgaa tgcctcctgg ggcacctcga cccgcccgat ctgcttcatc ttgcgcttgc
     4261 cctccttctg cttctcgagc agcttgcgct tgcgggtgat gtcgccgccg tagcacttgg
     4321 ccagcacgtc cttgcgcctg gcggagatcg tctcccgcgc gatcacccgc gagccgatcg
     4381 cggcctggac gaccacctcg tacagctggc gcgggatcag ctgcttcagc ctcgtcgcca
     4441 tggcccgccc gtaggcgtac gccttgtcct tgtggacgat ggccgagaag gcgtcgaccg
     4501 gctcgccctg caggagcagg tcgaccttga cgaggctggc ctcgcggtag ccgctcggct
     4561 cgtagtcgag cgaggcgtaa ccgcgcgtgc gcgacttcag cgcgtcgaag aagtcgaaga
     4621 tgatttccgc cagcggcaag gtgtagcgca gctcgacccg ctcggccgac aggtagtcca
     4681 tgccttgcag ttcaccgcgc cggctctggc acagctccat gatcgtgccg atgtactcgg
     4741 ccggggagat caccgtcgcg cgcaccacc
//
LOCUS       DASPYY010000582         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_800616, whole genome shotgun sequence.
ACCESSION   DASPYY010000582 DASPYY010000000
VERSION     DASPYY010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800616"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VEP73_09240"
     CDS             complement(<1..607)
                     /locus_tag="VEP73_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYT26649.1"
                     /translation="MDRLSRFLLRHRVAVGLCWLAVLVVGIATAGTVPGRLSTDFSLP
                     GAKSYQANQAILRVYGNGAQARPLVPVVTLPAGTTVDSPGVRQALERTFGAAARDPRL
                     RVVSWASTGDRDFVAADGRTTFGLVYPPPTGPNAPKLGPAVTAAMRGALPPSATLRVT
                     GFDELSAGDGGGGTGVLTETLLGGLGALAVLAFVFGSLLALV"
     gene            complement(751..1416)
                     /locus_tag="VEP73_09245"
     CDS             complement(751..1416)
                     /locus_tag="VEP73_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26650.1"
                     /translation="MVRVLLADDQNLVRAGFRVLLERSDDIEVVGEAADGAEAVDAAR
                     VVRPDVVLMDIRMPVLDGLEATRRIVADELLAGVRVVVLTTFELDEYVFEALRAGASG
                     FLLKDIDPDDLRQAVRVVAGGNALLSPSVTRRLITEFATRPARVPSDSARLEVLTVRE
                     REVLALVGGGLSNEEIGRRLFMSPATAKTHVSRAMAKLGAHDRAQLVVAAYETGLVAP
                     GQS"
     gene            complement(1423..>2428)
                     /locus_tag="VEP73_09250"
     CDS             complement(1423..>2428)
                     /locus_tag="VEP73_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYT26651.1"
                     /translation="ARRRWPLGVLLASTAILFVYYSLDYPGVSPAFPLAVALYTAAVA
                     GYFRWGLLITAFYMSAGILVVGVREGTPTEEVFASFLPQTSLLVTLLLLGEAVRSRRA
                     WLAEVRERLARAAAERKREAASRVEQERLRIARELHDVMAHTISVIAVQAGVASDVLV
                     DAPDTARQALGTIRAASREAMAELKATVGVLRDDDGQAAPLAPTPGLGQLDSLLETAR
                     RGGLRVESVVAGGRRPLPAAVDLTAYRIVQESLTNVARHAQATTATVAVRYEPDGVVI
                     EVCDDGRGAAGAPAAGQRGHGLAGMAERAAAVGGRLEAGPRPGGGFRVRSSLPAGRGV
                     "
BASE COUNT          339 a          935 c          877 g          277 t
ORIGIN      
        1 gaacgagggc gagcagcgag ccgaacacga aggccagcac ggccagggcg ccgagcccgc
       61 caagcagggt ctcggtgagc acgccggtgc cgccaccgcc gtcgccggcg gacagctcgt
      121 cgaagccggt cacccgcagg gtcgccgacg gcggcagggc gccgcgcatc gccgcggtca
      181 ccgccgggcc gagcttgggg gcgttggggc cggtcggcgg cgggtacacc agcccgaagg
      241 tcgtccgccc gtcggcggcg acgaagtcgc ggtcgccggt cgacgcccag gagacgaccc
      301 gcaggcgcgg gtcccgggcg gccgcaccga acgtccgctc cagcgcctgc cggacaccgg
      361 ggctgtccac ggtggtgcct gccggcagcg tgacgaccgg caccagcggg cgggcctggg
      421 cgccgttgcc gtagacgcgg aggatggcct ggttggcttg gtagctcttg gccccgggca
      481 gcgagaagtc ggtggagagc cggccgggca ccgtgccggc ggtcgcgatg ccgacgacca
      541 ggacggcgag ccagcacagc ccgacggcca cccggtggcg gagcaggaac ctcgacagtc
      601 gatccatggc ggcgcctttc gctgatcact cggtcttgga cacggagcga cggtacgggc
      661 cggcggcgca gacgtcgtcc gaccagcggc gcgaggttgc tgcaccgttc ggggtaggcg
      721 ccggggcgtc cgctgcaccc cgcggggtag tcaggactgg ccgggggcga ccaggccggt
      781 ctcgtaggcg gccacgacca gctgggcgcg gtcgtgggct ccgagcttgg ccatggcccg
      841 gctcacgtgg gtcttggcgg tcgccgggct catgaacagt cgccggccga tctcctcgtt
      901 ggacagccca ccgcccacca gggccagcac ctcacgctcc cgaacggtca gcacctccag
      961 ccgcgcggag tcgctcggca cccgcgccgg ccgggtcgcg aactcggtga tcagtcggcg
     1021 ggtgacgctc ggcgacaata gcgcgttgcc gccggctacc acccgcaccg cctggcgcag
     1081 gtcgtcgggg tcgatgtcct tgagcaagaa gccgctcgct ccggcgcgca gcgcctcgaa
     1141 gacatactcg tcgagctcga aggtggtcag cacgaccacg cgcacaccag cgagcagctc
     1201 gtcggcgacg atccgcctgg tcgcctcgag accgtccagc accggcatgc gaatgtccat
     1261 gagcaccacg tcgggccgca ccaccctggc cgcgtcgacc gcctcggcgc cgtcggcggc
     1321 ctccccgacc acctcgatgt cgtcgctgcg ctccagcagc acccggaacc cggcccggac
     1381 caggttctga tcgtcggcga gcagcacccg gaccaccgtg gctcacaccc cccgcccggc
     1441 cgggagcgac gatctgaccc ggaatccgcc gcctggccgc gggccggcct ccagccgtcc
     1501 gccgaccgcc gccgcccgct ccgccatccc cgccaggccg tggccgcgct gcccggcggc
     1561 gggtgcgccg gccgcgccgc gcccgtcatc gcagacctcg atgacgacgc cgtcgggctc
     1621 gtagcggacc gccacggtcg cggtggtcgc ctgggcatgg cgggccacgt tggtgagcga
     1681 ctcctgcacg atgcggtagg cggtcaggtc gaccgccgct ggcagcggcc gccggccgcc
     1741 ggccaccacg ctctcgacgc gcagcccccc gcgacgggcg gtctcgagca ggctgtcaag
     1801 ctggccgagg ccgggcgtcg gcgccagcgg ggcggcctgg ccgtcgtcgt cgcgcagcac
     1861 gccgacggtg gccttcagct cggccatcgc ctcccggctg gccgcccgga tcgtgccgag
     1921 cgcctgccgg gccgtatcgg gggcgtcgac cagcacgtcg gaggccaccc cggcctggac
     1981 ggcgatgacc gagatcgtgt gcgccatgac atcgtgcagc tcacgggcga tccgcagccg
     2041 ctcctgttcg acccggctcg ccgcttcccg cttgcgctcc gccgccgcgc gagccagccg
     2101 ctcgcgcacc tcagccagcc acgcccggcg gctgcgcacc gcctcgccga gcagcagcag
     2161 ggtcaccagc agcgacgtct gcggcaggaa gctggcgaac acctcctccg tcggcgtccc
     2221 ctcccggacg ccgacgacga gaatacccgc ggacatgtag aacgcggtga tcagcagccc
     2281 ccagcggaaa tacccggcca ccgcggccgt gtacagcgcg accgccagcg ggaacgccgg
     2341 gctgacgccc gggtagtcga gcgagtagta gacgaagagg atcgcggtcg acgccagcag
     2401 cacacccagc ggccaccgtc gcctggcc
//
LOCUS       DASPYY010000583         6516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2486678, whole genome shotgun sequence.
ACCESSION   DASPYY010000583 DASPYY010000000
VERSION     DASPYY010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486678"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2005
                     /gene="glgX"
                     /locus_tag="VEP73_09255"
     CDS             <1..2005
                     /gene="glgX"
                     /locus_tag="VEP73_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYT26652.1"
                     /translation="AGGERRLELPEVTAHVWHGYVPEVGPGQRYGFRVHGPYDPDRGH
                     RFNPAKLLLDPYARAVEGRVDWDDSVFGYRVGADDLVPDDRDSAPHVPRSVVVHDGFP
                     WGDDRRPDVPWADTVIYEAHLRGFTMRHPDVPVHQRGTYAAMGSPAVVDHLRRLGVTT
                     LELLPVHHYVSEHGVLERSLTNYWGYNSMGFFAPEARYSSAGTGGEQVREFKAMVRNL
                     HAAGIEVILDVVYNHTAEGNQLGPTLSFRGLDNRSYYRTVDLNPRYYMDYTGCGNTLN
                     VRHPQVLTLIMDSLRYWVEEMHVDGFRFDLASALARSFHEVDRLSAFFDLIHQDPVVS
                     RVKLIAEPWDVGEGGYQVGNFPVLWTEWNGRYRDTIRDFWRVARTGVADIAYRLTGSS
                     DLYKDDGRRPYASINFVTAHDGFTLHDLVSYDHKHNEANGEGNRDGSDDNRSWNCGVE
                     GPTDDPEVVALRERQKRNFLCSVLLSAGVPMLVAGDELSRTQQGNNNAYCQDNEISWV
                     DWELDDRAESLAEFTRSIIELRAGHPVFRRRKFYQGQAIHGSGVKDIGWFTPDGAEMD
                     QADWAAPDVQTLGVFLNGEEIPDRGRRGERIADDSFLLLFNGGPEPALFALPGRPWGK
                     EYELVVDTGLGFVLPAGAYAPTYLAGEELSMASRSVAVLRKRA"
     gene            2068..2418
                     /locus_tag="VEP73_09260"
     CDS             2068..2418
                     /locus_tag="VEP73_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HYT26653.1"
                     /translation="MGSLLEGVAVLAWVYLAVAILAEVSGTVALRFVSGLSRPVPLAI
                     VVAGYGLAFYLLMRTLKLLSLGLTYAVWAGAGTALVAIVGMLALDEPVNAPKLLGIVL
                     VIAGIVTLNLGGAH"
     gene            2415..3101
                     /locus_tag="VEP73_09265"
     CDS             2415..3101
                     /locus_tag="VEP73_09265"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYT26654.1"
                     /translation="MTAAGAPGPDRTDGRRERGLRRRRAILEATLSIIGEQGTAAVTH
                     RAVAAAAGVPLAATTYYFASKDDLLDQALRYAAEEQLAALEGAPPPATGVRTVADAVE
                     RVWTVRRSYLARGRERALAHYEIYLEAARRPALRAVAHEWNEACVRQLAPGLASLGIA
                     EPEAAARLVLAALDGFLISDLATHRPGFADDVLRPGVGRLLAALGGGQASPPAGTVGG
                     EAADHQEEPS"
     gene            3098..5380
                     /gene="treY"
                     /locus_tag="VEP73_09270"
     CDS             3098..5380
                     /gene="treY"
                     /locus_tag="VEP73_09270"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HYT26655.1"
                     /translation="MTVHLEPSSTYRLQLSARFPFEAARARVGYLADLGASHLYASPV
                     LRARSGSPHGYDVVDPTEVAPELGGEAGLAALVGTLRAHGMGLVVDLVPNHMAASVEN
                     PWWVDTLRHGRASPYARVFDVDWEAAGGRLRLPVLGGPLEQVAGEIEVGGGWVRYHEQ
                     RFPLAPGTSRLGEQHYELVDWRRAAVDGNYRRFFDVNELVGVRQEDPEVFELTHATAL
                     RLVADGLVDGFRIDHVDGLADPAGYLERLAGRGVPWVVVEKILESSERLPAWPVAGST
                     GYEFLALVDGLFVDPEAAGAFTDRYVRLTGRDPNSPALAVRCKREVLGGSFGGEVGGV
                     ARHLPGDLAANRAAVVELAAQYPVYRTYVTDRVSAFDRAVIADARGACEDRLWTGVPP
                     DEAPGGGALDRLVAALHLDDPAPQAVRRFQQLTGPAMAKGVEDTALYRDARMVGRNEV
                     GGDLGRFGRSVEELHAANAEREERWPSSMLATSTHDTKRGEDVRARLAVLTWHPERWW
                     ELAERWTRRLAGGEGGRGTAVEAADALLLWQTMVGAWPLERERCRAYMEKAIREAQVH
                     TEWANPDPGYEGAVRDLVDRAYADPALRAELAALVAEVAPAGRVNAAGLALLRLTSPG
                     VPDTYQGTEVEQLVLVDPDNRRPVAFTPDGSLKFRVTRAALRLRRERPELFTGYRPLA
                     APDRLVAFARGGDRLAVVVRRLGGPPEGPAGGGAPEGGQVELPEGPWRHLLSGATLRG
                     GPTAAEELAAEVPHALLVRD"
     gene            complement(5437..>6516)
                     /locus_tag="VEP73_09275"
     CDS             complement(5437..>6516)
                     /locus_tag="VEP73_09275"
                     /inference="COORDINATES: protein motif:HMM:NF023284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HYT26656.1"
                     /translation="RLGSGYRSYVRLKLQRVMDDVAEIVAGTWAYPPESGQASFVRTA
                     MLALLERRFPAAETGEVVTQPVMAFLKSFDLAFTERRLRFVIQGVNAGYGDAAAGVGG
                     GTPPIGPLGDAERKQLDTVKRELYGFIEELWAAVTPATIRRQLGEEPFGLFSEDQLAE
                     PLRREVEPQAFADQHRQQLDALLDRLKDHLDATLGTFPERLWTRFVEVTAGWDQLRQL
                     LLVRFLGFPIWDTLLLPIVELSGVRQFSQIEVVRISPRDGDPRLLPAKRLMGAATHHF
                     GAFFSRKAREHDYLWGRLDGVEQLLDVVAPDIEQSWYRRAFEAVLDEEGSSLPLAAAE
                     IQGVRGKVSTLPAVAEPVSKTPAAR"
BASE COUNT          831 a         2436 c         2383 g          866 t
ORIGIN      
        1 ggccggcggc gagcgccgcc tcgagctgcc cgaggtcacc gcgcacgtct ggcacgggta
       61 cgtgcccgag gtgggtcccg gccagcgcta cggcttccgg gtccacgggc cctacgaccc
      121 cgacaggggg caccgcttca acccggccaa gctgctgctg gacccgtacg cgagggcggt
      181 ggagggccgc gtcgactggg acgactcggt gttcgggtac cgggtcggcg ccgacgacct
      241 ggtccccgac gaccgcgact cggcgccgca cgtgcccagg tcggtcgttg tccacgacgg
      301 cttcccctgg ggcgacgacc gccgcccgga cgtgccctgg gccgacacgg tcatctacga
      361 ggcgcacctg cgcggcttca ccatgcgcca ccccgacgtc ccggtccacc agcgcggcac
      421 ctacgccgcg atgggctccc cggcggtcgt cgaccacctc cgccggctcg gggtcacgac
      481 cctcgagctc ctgccggtcc accactacgt ctccgagcac ggcgtgctcg agcggagcct
      541 caccaactac tggggctaca actccatggg gttcttcgcg cccgaggcgc gctactcgtc
      601 agccgggaca gggggcgagc aggtgcgcga gttcaaggcg atggtccgca acctgcacgc
      661 cgccggcatc gaggtgatcc tcgacgtggt ctacaaccac accgccgagg gcaaccagct
      721 gggcccgacc ctgtccttcc gcgggctcga caaccggtcc tactaccgca ccgtcgacct
      781 gaacccgcgc tactacatgg actacaccgg ctgcggcaac accctgaacg tgcgccaccc
      841 ccaggtgctc accctcatca tggacagcct gcgctactgg gtggaggaga tgcacgtcga
      901 cgggttccgc ttcgacctgg cgagcgcgct ggcgcgcagc ttccacgagg tcgaccgcct
      961 gtcggccttc ttcgacctga tccaccagga cccggtggtc agccgggtga agctgatcgc
     1021 cgagccctgg gacgtgggcg agggcggcta ccaggtgggc aacttcccgg tgctgtggac
     1081 cgagtggaac gggcgctacc gcgacaccat ccgggacttc tggcgggtcg cccggaccgg
     1141 cgtggccgac atcgcctacc ggctcaccgg ctcgtccgac ctgtacaagg acgacggccg
     1201 ccgcccctac gcgagcatca acttcgtcac cgcgcacgac gggttcacgc tgcacgacct
     1261 cgtctcctac gaccacaagc acaacgaggc caacggcgag ggcaaccgcg acggcagcga
     1321 cgacaaccgc tcctggaact gcggggtgga ggggcccacc gacgatccgg aggtcgtggc
     1381 gctgcgggag cgccagaagc ggaacttcct gtgcagcgtg ctgttgtcgg ccggggtgcc
     1441 gatgctcgtc gccggcgacg agctctcccg cacccagcag ggcaacaaca acgcctactg
     1501 ccaggacaac gagatctcct gggtggactg ggagctcgac gaccgggccg agtcgctcgc
     1561 cgagttcacc cgctcgatca tcgagctccg cgcgggccac ccggtcttcc gccggcgcaa
     1621 gttctaccag ggccaggcga tccacggctc gggcgtcaag gacatcggct ggttcacccc
     1681 ggacggcgcg gagatggacc aggccgactg ggcggcgccc gacgtccaga cgctcggcgt
     1741 gttcctgaac ggcgaggaga tcccggacag ggggcggcgc ggcgagcgca tcgccgacga
     1801 cagcttcctg ctgctcttca acggcggccc cgaaccggcc ctgttcgccc tgcccggccg
     1861 cccatggggc aaggagtacg agctggtggt ggacaccggg ctcgggttcg tgctcccggc
     1921 cggggcctac gccccgacct acctggccgg cgaggagctg tcgatggcct cccgctcggt
     1981 cgccgtcctg cgcaagcgcg cctgacgcgc tccccggtcc gccgaggccc cgcgctcgct
     2041 ggaagtggta cgatcgtacc agttatcttg gggtcgcttc tcgagggggt cgccgtgctc
     2101 gcgtgggtct acctggcagt cgccatcctg gccgaggtga gcgggacggt cgcgctgcgg
     2161 ttcgtcagcg gcctgtcgcg cccggtgccc ctcgcgatcg tcgtcgccgg ctacgggctc
     2221 gccttctacc tgctcatgcg cacgctgaag ctgctctcgc tcggcctcac ctacgccgtc
     2281 tgggccgggg ccggcaccgc gctcgtcgcg atcgtcggca tgctcgccct cgacgagccg
     2341 gtgaacgcgc ccaagctgct cggcatcgtc ctcgtgatcg ccggcatcgt gacgctgaac
     2401 ctcggcggag cgcattgacc gcggcgggcg ctcccggccc ggaccgcacc gacgggcggc
     2461 gggagcgggg gctccgccgc cgccgggcga tcctcgaggc gacgctctcg atcatcggcg
     2521 agcagggcac cgcggccgtc acccaccgtg ccgtggcggc cgcggccggc gtgccgctcg
     2581 ccgccacgac gtactacttc gcctcaaagg acgacctgct cgaccaggcg ctgcggtacg
     2641 ccgccgagga gcagctggcc gccctggagg gcgcgccgcc gcccgcgacg ggcgtccgca
     2701 cggtcgccga tgcggtcgag cgggtctgga ccgtccggcg gagctatctc gcgcgcggcc
     2761 gggagcgggc gctcgcgcac tacgagatct acctggaggc cgcgcgccgc ccggccctgc
     2821 gggcggtggc gcacgagtgg aacgaggcgt gcgtccgcca gctcgcgccc ggcctcgcga
     2881 gcctcggcat cgccgagccc gaggccgccg cccgcctggt cctcgcggcg ctggacgggt
     2941 tcctgatctc cgacctcgcc acccaccggc ccggcttcgc cgacgacgtc ctgcgcccgg
     3001 gcgtcggccg cctgctcgcc gcgctgggcg gcgggcaggc ctccccgccg gccggtaccg
     3061 tcggggggga ggcagccgac caccaggagg agccgtcctg accgtccacc tcgagccgag
     3121 ctccacctac cgcctgcagc tctccgcccg gttcccgttc gaggccgccc gtgcccgggt
     3181 cggctacctc gccgacctcg gcgcctccca cctgtacgcg tcgccggtcc tccgtgcccg
     3241 ctccggctcg ccccacggct acgacgtcgt cgaccccacc gaggtcgctc ccgagctcgg
     3301 cggcgaggcc ggcctcgccg cgctggtcgg gacgctgcgc gcccacggca tgggcctggt
     3361 ggtcgacctc gtcccgaacc acatggcggc ctcggtcgag aacccctggt gggtcgacac
     3421 cctgcgccac gggcgggcct cgccctacgc cagggtcttc gacgtcgact gggaggcggc
     3481 cggcggccgg ctgcggctgc cggtcctcgg cggcccgctc gagcaggtgg ccggcgagat
     3541 cgaggtcggc ggcgggtggg tccgctacca cgagcagcgc ttcccgctcg ccccggggac
     3601 gagccgcctc ggcgagcagc actacgagct ggtcgactgg cgccgcgccg cggtggacgg
     3661 caactaccgc cggttcttcg acgtcaacga gctggtcggg gtgcgccagg aggacccgga
     3721 ggtcttcgag ctgacccacg cgacggccct ccggctcgtg gccgacgggc tggtcgacgg
     3781 gttccgcatc gaccacgtcg acggcctggc ggacccggca ggctacctgg agcgcctggc
     3841 cggccggggc gtcccctggg tcgtggtgga gaagatcctc gagtcgagcg agcgcctgcc
     3901 cgcctggccg gtcgcgggca gcaccggcta cgagttcctg gccctggtcg acgggctctt
     3961 cgtcgacccg gaggcggccg gcgcgttcac cgaccggtac gtgcggctga ccggccggga
     4021 ccccaactcg cccgcgctcg cggtccgctg caagcgcgag gtgctcggcg gctcgttcgg
     4081 gggcgaggtc ggcggcgtcg cgcgccacct gccgggggac ctggccgcca accgggcggc
     4141 cgtcgtcgag ctggccgccc agtacccggt ctaccggacg tacgtgaccg accgggtgtc
     4201 cgccttcgac cgggcggtga tcgccgacgc ccgcggcgcc tgcgaggacc gcctgtggac
     4261 gggcgtgccg ccggacgagg cgccgggcgg cggggcactc gaccggctcg tcgccgccct
     4321 gcacctcgac gacccggcgc cccaggccgt ccggcggttc cagcagctca ccgggccggc
     4381 catggcaaag ggggtcgagg acaccgccct gtaccgggac gccaggatgg tggggcgcaa
     4441 cgaggtgggc ggcgacctcg gccggttcgg ccgctcggtg gaggagctcc acgcggccaa
     4501 cgccgagcgc gaggagcggt ggccgtccag catgctcgcc acctcgacgc acgacaccaa
     4561 gcgcggggag gacgtgcggg ccaggctcgc ggtgctcacc tggcacccgg agcgctggtg
     4621 ggagctggcc gagcgctgga cgcgccggct ggccggtggc gagggcggcc gcgggacggc
     4681 ggtggaggcc gccgacgccc tgctgctgtg gcagacgatg gtcggcgcct ggccgctcga
     4741 gcgggagcgc tgccgcgcct acatggagaa ggccatccgg gaggcgcagg tgcacaccga
     4801 gtgggcgaac cccgaccccg ggtacgaggg ggccgtgcgc gacctcgtcg accgcgccta
     4861 cgccgatccg gccctccgtg ccgagctggc cgccctggtc gccgaggtcg cgccggcggg
     4921 cagggtcaac gccgccggcc tcgcgctgct caggctcacg agcccggggg tgccggacac
     4981 ctaccagggc accgaggtgg agcagctcgt gctggtggac cccgacaacc gccggccggt
     5041 cgcgttcacc cccgacggct cgctcaagtt ccgggtcacc cgggccgcgc tgcgcctgcg
     5101 ccgcgagcgg ccggagctgt tcaccggcta ccgtccgctg gccgcccccg accggctggt
     5161 cgcgttcgcc cgcggcggcg accgcctcgc ggtcgtggtg cgccgcctcg gcgggccgcc
     5221 ggagggccca gcgggcggcg gggcgccgga gggcggccag gtcgagctgc ccgagggccc
     5281 gtggcgccac ctgctctccg gcgccaccct gcgcggcggc ccgaccgcgg cggaggagct
     5341 ggccgccgag gtcccccacg cgctgctggt gcgcgactga gcacctctgg agcgccatga
     5401 ggcggaggtt ccttccacgt ccacggtcct ggtgcgctac cgggcggccg gggtcttcga
     5461 gacgggctcc gcgaccgctg gcagggtcga gaccttgccg cgcacgccct ggatctccgc
     5521 cgcggccaag ggcagggagg agccctcttc gtcgagcacg gcctcgaagg cgcgccggta
     5581 ccaggactgc tcgatgtcgg gcgcgacgac gtccaggagc tgctcgacgc cgtccaggcg
     5641 gccccagagg tagtcgtgct cgcgggcctt gcggctgaag aaggcgccga agtggtgtgt
     5701 ggccgcgccc atcaggcgtt tggctggcag caggcggggg tcgccgtcgc gcgggctgat
     5761 gcggacgacc tcgatctggc tgaactggcg cacgccgctc agctcgacga tcggcaggag
     5821 cagcgtgtcc cagatcggga agcccaggaa gcggacgagc aggagctgcc gcagctggtc
     5881 ccagccggcc gttacctcca caaaccgcgt ccacagccgc tcggggaagg tccccagggt
     5941 ggcgtccagg tggtccttga ggcggtcgag cagggcgtcg agctgttggc ggtgctggtc
     6001 ggcgaacgcc tggggctcca cctcgcggcg cagcggctcg gcgagctggt cctcgctgaa
     6061 cagcccgaag ggttcctcgc cgagctgccg tcggatggtg gccggcgtga ccgcggccca
     6121 cagctcctcg atgaagccgt acagctcccg cttgacggtg tcgagttgct tgcgctcggc
     6181 gtcgccgagc ggcccgatcg gcggggtgcc gccaccgacg cccgccgctg cgtccccgta
     6241 gccggcgttg accccctgga tgacgaagcg gaggcggcgc tcggtgaacg ccaggtcgaa
     6301 gctcttgagg aaggccatga ccggctgggt gacgacctcc ccggtttcgg cggcggggaa
     6361 cctgcgctcc agcagggcca gcatcgccgt gcggacgaag ctcgcctgtc cggactcggg
     6421 gggataggcc caggtcccgg cgacgatctc ggcgacgtcg tccatgaccc gttgcagctt
     6481 cagccgcaca tagctgcggt agccgctgcc caggcg
//
LOCUS       DASPYY010000584         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2486800, whole genome shotgun sequence.
ACCESSION   DASPYY010000584 DASPYY010000000
VERSION     DASPYY010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486800"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VEP73_09280"
     CDS             complement(<1..673)
                     /locus_tag="VEP73_09280"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYT26657.1"
                     /translation="MDHDAKLELGRELTRRLIARHGEGAIVAVGVHGPVARGDDTDGS
                     DLRLAVITAGPDVEVGDRLLRHRGTVVDLGAIWADAYLEEAGHIGPTWPLAADQYLHH
                     LALHDPTGFFHKLRHVHETAVSSAAEEAFAAAAGYDLVQLISWEGTARTAELHGDVPG
                     ALLAVKEAAVLCALVVGLATRTAYRDAAHAVRSVALTEAAPPGFADPYRRLLSPATDP
                     AGAVIA"
     gene            complement(790..>1613)
                     /locus_tag="VEP73_09285"
     CDS             complement(790..>1613)
                     /locus_tag="VEP73_09285"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26658.1"
                     /translation="TGSTEVGRRVQELSAGTVKKVLLELGGKSANVVLEDADLDLAVD
                     GAVYAIFFHQGQVCTAGSRLLVARPVYGEVVARVVETAERIKVGPAADKASDMGPLVS
                     QEQLERVERYVRIGQEEGAKLATGGERVAVPGHEGGYYFAPTVFTDVDPGSRIAQEEI
                     FGPVLCVIPFDGDDQAVAIANDSAYGLAGAVWSRDPARAVKVAERLRTGTVWVNDYHL
                     LNPRYPFGGYKQSGLGRELGEQGYLEYTQTKHVHVDISQQRSRHRWFDVTVPRRR"
BASE COUNT          207 a          592 c          597 g          217 t
ORIGIN      
        1 gggcgatgac cgcgccggcc gggtcggtgg cgggggagag caggcgccgg taggggtcgg
       61 cgaagccggg cggcgccgcc tcggtcagcg ccaccgaccg cacggcgtgc gccgcgtcgc
      121 ggtacgccgt cctggtcgcg agcccgacga cgagcgcgca cagcacggcc gcctccttca
      181 ccgccagcag cgcgcctggc acgtcgccgt gcagctcggc ggttcgcgcc gtgccctccc
      241 aggagatgag ctgcaccagg tcgtagccgg cggcggccgc gaacgcctcc tccgcggcgg
      301 acgagacggc ggtctcgtgc acgtgccgca gcttgtggaa gaaccctgtc gggtcgtgga
      361 gggccaggtg gtggaggtac tggtcggcgg cgaggggcca ggtggggccg atgtggcccg
      421 cctcctcgag gtaggcatcg gcccagatgg cgccgaggtc gaccacggtg cctcggtggc
      481 ggaggagccg gtcgccgacc tcgacgtccg ggccggcggt gatgacggcg aggcgcaggt
      541 cggacccgtc ggtgtcgtca ccgcgcgcca ccgggccgtg gacgccgacg gccacgatcg
      601 cgccctcgcc gtggcgggcg atgaggcggc gggtgagctc ccggccgagc tcgagcttcg
      661 cgtcgtggtc caagggcggc ctccggtcgg cggatgccgg caaggctacc cgagccggtg
      721 cggcgggtgg ctcgcgcgcc atgggtacgc cgcgacacga ggcggtccga actacgtggg
      781 acgcatgaac taccgtcggc gggggacggt cacgtcgaac cagcggtggc gcgagcgctg
      841 ctgcgagatg tcgacgtgga cgtgcttggt ctgcgtgtac tcgaggtagc cctgctcgcc
      901 cagctcgcgc ccgagacccg actgcttgta ccccccgaac gggtacctcg ggttcagcag
      961 gtggtagtcg ttcacccaca ccgtcccggt gcgcagccgc tcggcgacct tgaccgcgcg
     1021 ggccgggtcg cggctccaca ccgccccggc gagcccgtac gccgagtcgt tggcgatcgc
     1081 gaccgcctgg tcgtcgccgt cgaacgggat gacgcacagc accggcccga agatctcctc
     1141 ctgggcgatc cgcgaccccg ggtccacgtc ggtgaacacc gtcggggcga agtagtagcc
     1201 gccctcgtga ccgggcacgg cgacccgctc cccgccggtc gcgagcttgg cgccctcctc
     1261 ctgcccgatc cgcacgtacc gttccacccg ctcgagctgt tcctgcgaga ccagcgggcc
     1321 catgtcggac gccttgtcgg ccgccggtcc caccttgatc cgctcggcgg tctccaccac
     1381 gcgtgcgacc acctcgccgt acacggggcg ggcgacgagc aggcggctcc ccgcggtgca
     1441 gacctgcccc tggtggaaga agatcgcgta gaccgccccg tcgaccgcga ggtcgaggtc
     1501 ggcgtcctcc agcacgacgt tcgccgactt gccgccgagc tcgagcagca ccttcttgac
     1561 cgtcccggcc gacagctcct gcacccggcg gcccacctcg gtcgagccgg tga
//
LOCUS       DASPYY010000585         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_661850, whole genome shotgun sequence.
ACCESSION   DASPYY010000585 DASPYY010000000
VERSION     DASPYY010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661850"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..750
                     /locus_tag="VEP73_09290"
     CDS             469..750
                     /locus_tag="VEP73_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HYT26659.1"
                     /translation="MAEATPPPLAERDRAVLAFERQWWKYAGAKEEAIRRQFGLSPTG
                     YYQLLTRLIDDPAALEADPMLVKRLQRQRAARRRRRASRTLGAQGPGGG"
     gene            818..1339
                     /locus_tag="VEP73_09295"
     CDS             818..1339
                     /locus_tag="VEP73_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HYT26660.1"
                     /translation="MLVVFLGLFWGIDRVGGDAGKAADARRPAAAVATASSAAATTTG
                     VPATTGRPATTAAPTGTAPPTTREPAATGSTLRPAAGVTVQVLNGTLRTGEARRAADM
                     LSKAGYDVIATQLAFGGYPVSRVYYNEGHLEDAIALRQRFPVFQLLLPASESRTGLSR
                     KVDLSAVVGRNFR"
     gene            1336..2142
                     /gene="otsB"
                     /locus_tag="VEP73_09300"
     CDS             1336..2142
                     /gene="otsB"
                     /locus_tag="VEP73_09300"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYT26661.1"
                     /translation="MTAPDPWDLVATIAREAERAALCLDYDGTLAPIVPDPEAAVPLP
                     GVPALLARLARRFAAVALISGRPAAYLVERVGAPGARYLGLYGAEEVVDGRVNVDPEV
                     EAVRPEVRAALAELRAHPAVVESGAWVEDKGLAVAVHLRRVADPGRRAPVVEEAAREV
                     AGRHGLEVLPGRLVWELRPPTGRDKGSAVRQVVAESGAGFLVVAGDDVGDLAAFRAAV
                     ALAADGVRALRVAVRSPESPPELLEEADLVVDGPEGVRDLLESLAGAAGP"
     gene            2173..3438
                     /locus_tag="VEP73_09305"
     CDS             2173..3438
                     /locus_tag="VEP73_09305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26662.1"
                     /translation="MRTRPRLLSDFTPLRESRQYRLLYAGELVALLGSQLTVVAVPLQ
                     VYLLTRSSLAVGLIGLAQLGPLLACSLIGGAVVDAVDRRVLLVWVQLARGALSAGLAV
                     NALRPHPAIWPLYVLTAAGAGLQALDAPARTAAVPALVRRELVPAAAALHQILMQVSM
                     VAGPAVAGLVVARASLAAAYGIDVATFTLASLLAVALRPLPPGEGGTRAGVASVREGL
                     RFLRGRRVLQGAFLVDLNAMVFGMPRALFPALGTGLFHGGPGTVGLLYAAPGAGALVG
                     ALTAGWVGGVRRQGRAVLVAVAAWGAAVTLFGVVAWLPLALGLLALAGAADVVSAVFR
                     STILQLTVPDGLRGRLAAINIAVVTGGPRLGDAEAGAVAALAGPQFSVVSGGLACLAG
                     VALLAWRLPELGRTAIEPDAPLPDGQPAP"
BASE COUNT          365 a         1356 c         1369 g          477 t
ORIGIN      
        1 ccggtgcgac ggagagcgtc atcaagggaa ggaggacggc cgcgagcagc aggacgcgcc
       61 gtcctcgaag cggctcgctc cggctggggg accgtcgagc gcatgacggc aagcctggct
      121 cctttcctca ggcctgcggg acgaccggcg agcgtgtgga ggaaggggga tgctcgcgtt
      181 tcccgcatcc cgcgcggtcg cgggactggc gcggtaccga ccgttacgaa acgtggctgc
      241 caagtaacca attcgtgatc aggcgcgcgc aggttacccc tcggcgcatc ccctgccaaa
      301 tcctgtggtc acgaccgcgg ccagcgaccc cgtggtttgc ctccccccag atgtggggtt
      361 atggtgatga cccgccccaa gatgttgtga ccgtccgggg cggccctgct ggtcagccaa
      421 ccggcggctg cggagaacta ccgacgctga ctagagagga gccgagccat ggccgaggcg
      481 acgccgccac cgctcgcgga gcgggaccgg gccgttctcg ccttcgagcg tcagtggtgg
      541 aagtatgccg gggccaagga agaggcgatc cggcgccagt tcgggctcag ccccaccggg
      601 tactaccagc tcctcaccag gctcatcgac gacccggcgg cgctcgaggc cgaccccatg
      661 ctggtgaagc ggctgcagcg ccagcgcgcg gcccggcgcc gtcggcgcgc gtcccgtacc
      721 ctcggggcgc aaggtcccgg cggcggctag ccgcgccaga actcgcagcg acagggaggt
      781 caggttgcga cccggtccga ccgctcgagc ggcggcggtg ctcgtcgtat tcctcgggct
      841 gttctggggg atcgaccgcg tcggcggcga cgccgggaag gccgccgacg cgagacggcc
      901 ggccgccgcg gtcgccacgg cgtccagcgc cgccgcgacc acgaccgggg tgcccgcgac
      961 gaccggcagg ccggcgacca ccgccgcccc caccggcacg gctccgccga ccacccggga
     1021 gccggccgcc accggctcga cgctgcggcc cgccgcgggc gtgaccgtgc aggtcctcaa
     1081 cgggaccttg aggaccggcg aggcccgccg ggcggccgac atgctctcga aggccggcta
     1141 cgacgtgatc gcgacccagc tcgccttcgg cgggtacccg gtctcccgcg tgtactacaa
     1201 cgagggccac ctcgaggacg cgatcgcgct ccggcagcgc ttcccggtct tccagctcct
     1261 cctccccgcc tcggagtcga ggaccgggct gtcgcgcaag gtggacctct ccgccgtggt
     1321 ggggcggaac ttccgatgac cgcgccagac ccgtgggacc tcgttgccac gatcgcccgg
     1381 gaggcggagc gggccgcgct ctgcctcgac tacgacggca ccctcgcgcc gatcgtgccc
     1441 gaccccgagg cggccgtgcc gctgccgggc gtcccggcgc tgctggcccg cctcgccagg
     1501 cggttcgcgg ccgtcgccct gatctccggc cggcccgccg cctacctggt cgagcgcgtg
     1561 ggcgcccccg gcgcccgcta cctcgggctg tacggggccg aggaggtcgt cgacggccgg
     1621 gtcaacgtgg atccggaggt ggaggcggtc cgcccggagg tgcgggcggc cctggccgag
     1681 ctccgtgcgc acccggcggt cgtcgagtcc ggcgcctggg tcgaggacaa ggggctcgcc
     1741 gtggccgtcc acctccgccg ggtcgccgac ccgggccgcc gggcgcccgt ggtcgaggag
     1801 gcggcccgcg aggtcgccgg gcggcacggg ctcgaggtgc tgcccggccg gctggtgtgg
     1861 gagctgcgcc cgccgaccgg ccgggacaag ggcagcgccg tccgccaggt ggtggccgag
     1921 tcgggcgccg gcttcctggt cgtggccggt gacgacgtcg gcgacctggc cgccttccgg
     1981 gccgccgtcg cgctggccgc cgacggcgtg cgtgccctgc gggtcgcggt gcggtcgccg
     2041 gagtcgcccc ccgagctgct cgaggaggcc gacctcgtgg tcgacggccc ggaaggcgtg
     2101 cgcgacctgc tcgagtccct ggcgggcgcc gccgggccct gagccggttg ctgtcgttgc
     2161 taccgtggtc gcatgcgcac ccggccgcgc ctcctcagcg atttcacccc gctcagggag
     2221 tcccgccagt accggctgct gtacgcgggc gagctggtcg ccctgctcgg cagccagctc
     2281 accgtggtcg cggtgccgct ccaggtctac ctgctgaccc gctcgtcgct ggcggtcggg
     2341 ctgatcgggc tggcgcagct cggaccgctg ctggcctgct cgctgatcgg cggcgcggtg
     2401 gtcgacgcgg tcgaccggcg cgtgctgctc gtgtgggtgc agctcgcccg cggcgcgctc
     2461 agcgccgggc tggcagtgaa cgcgctgcgg ccccacccgg cgatctggcc gctgtacgtg
     2521 ctcaccgcgg ccggcgcggg cctgcaggcg ctggacgcgc cggcacggac ggcggcggtg
     2581 cccgccctgg tccgcaggga gctcgtgccc gccgcggcgg cgctgcacca gatcctcatg
     2641 caggtcagca tggtcgcggg gccggcggtg gccgggctgg tcgtcgccag ggcgagcctg
     2701 gccgcggcgt acggcatcga cgtggccacc ttcaccctgg ccagcctgct cgccgtggcg
     2761 ctgcggccgc tgccgccggg cgagggcggg acccgggcgg gcgtcgcctc ggtccgcgag
     2821 ggcttgcgct tcctgcgcgg ccggcgcgtc ctgcagggcg cgttcctcgt cgacctgaac
     2881 gcgatggtgt tcggcatgcc gcgggcgctg ttccccgcgc tcggcaccgg gctgttccac
     2941 ggcgggccgg gcacggtcgg gctgctgtac gccgcgccgg gcgccggcgc gctcgtcggc
     3001 gcgctcacgg ccggctgggt cggcggcgtc cgcaggcagg ggcgcgccgt gctcgtcgcg
     3061 gtggccgcgt ggggcgcggc ggtcacgctg ttcggggtcg tcgcctggct gccgctggcg
     3121 ctcggcctgc tcgcgctcgc cggtgccgcc gacgtcgtct cggccgtgtt ccgcagcacg
     3181 atcctgcagc tcaccgtgcc cgacgggctg cgaggcaggc tcgcggcgat caacatcgcc
     3241 gtggtcaccg gcggcccccg gctgggcgac gccgaggccg gcgcggtcgc cgcgctcgcc
     3301 ggcccgcagt tctcggtggt ctccggcggg ctcgcgtgcc tcgcgggggt cgccctgctc
     3361 gcctggcgcc tgcccgagct cggccgcacc gcgatcgagc cggacgcgcc cctccccgac
     3421 ggccagcccg cgccctagcc ggtgccgtcg ccgcgcaggt gggccaggtc ctcgcgctgg
     3481 ctcgcgaacc agtcgtgcgg cggcagggcg ctcgccgcct cacgcagccg cttcgcccgc
     3541 tcggcccgct cgtcggccgg catggcc
//
LOCUS       DASPYY010000586         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1504860, whole genome shotgun sequence.
ACCESSION   DASPYY010000586 DASPYY010000000
VERSION     DASPYY010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504860"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="VEP73_09310"
     CDS             <1..2074
                     /locus_tag="VEP73_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26663.1"
                     /translation="AVYAAPIVLSGQATFAGYIRLDDTATWMALTDRVMEHGRSLDGL
                     APSTYEATLAFNLGSGYPVGVFLPLGIARELVGQDVAWVIQPYMAFLAGLLAAGLWSV
                     AGSLVRSPWLRALVAFLAAQSALLFGYYLWGGIKEVAAAALVVTVAGLAALVIRERLA
                     WRTLAPLAVASAALIGILSGGGAVWLGPILLATLVAAWRMLGVGAALGRAAALAAALA
                     ALSIPVIAPGGLLPPTSSPLTSETALGNLFRPLSAFRLFGVWPSGDFRADPTDPATAH
                     LLIAVVALAALVGIGLAVRARAWSLLLYVGAAVVAGLAIFVIGSPWVGGKALATASPA
                     LPLAAVAAGAALYAEGRRVEGGILMAVLAAGILWSNALAYRDVNLAPRDQLAELQEIG
                     ELIAGQGPTLMTEYQPYGVRHFLRNADPEAASELRRRRVPLLSGRTLRKGAYADTDRF
                     KLDGLEVYRTLVVRRSPAQSRPPLPYRLIWRGHYYEAWQRPAGSEGVVRDHLGLGNEV
                     DPGGVPKCADVLHLADEAGPNGRLAAVGRAPVVIVSLKQTSHPRAWNSPDYASALLPT
                     TPGAIKAQIRVSKPGGYEIWLGGSVRPQVDVAVDGRPVGSVREQLENSGQYVSLGTAQ
                     LDPGEHTVTIRFHGADLHPGSGGAPDPIGPLVLSQQDMADTRVVYFPPSEATRLCGKR
                     WDWIEALG"
     gene            2238..>2474
                     /locus_tag="VEP73_09315"
     CDS             2238..>2474
                     /locus_tag="VEP73_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26664.1"
                     /translation="MIRARLPLAVLPASLVALVAVAGCGGGSSSTSTSPSTGGATGGG
                     AATIDVAKNAQLGQILTDSKGNTVYVFAKDTNGKS"
BASE COUNT          314 a          914 c          884 g          362 t
ORIGIN      
        1 cgccgtctac gccgccccga tcgtcctctc cggccaggcc accttcgccg gttacatccg
       61 gctcgatgac acggccacct ggatggcgct caccgaccgg gtgatggagc acgggcgaag
      121 cctcgacggc ctcgcgccgt cgacgtatga ggcgacgctg gcgttcaacc tgggctcggg
      181 ctatcccgtc ggcgtcttcc tgccgctcgg gatcgcccgc gagctggtgg gccaggacgt
      241 cgcctgggtc atccagccct acatggcctt cctcgccggg ctgctggccg cggggctgtg
      301 gagcgtggcc ggctcgctcg tccgctcacc gtggctgcgc gccctggtcg ccttcctcgc
      361 ggcccagtcg gcgctgttgt tcggctacta cctgtggggc gggatcaagg aggtggccgc
      421 ggcggccctc gtcgtcacgg ttgccgggct cgccgcgctc gtgatccgcg agcgcctcgc
      481 atggcggacg ctggcgccgc tggcggtggc gagcgcggcg ctgatcggca tcctcagcgg
      541 tggcggcgcg gtgtggctcg gcccgatcct gctggcgacc ctggtcgctg cgtggcggat
      601 gctcggcgtc ggcgccgcgc tgggcagggc cgccgccctc gccgccgccc tcgccgccct
      661 cagcatcccc gtgatcgcgc ccggggggct gctgccgcca acctcgtcgc cgctgaccag
      721 cgaaaccgcc ctcggcaacc tgttccgccc cctcagcgcc ttccggctgt tcggcgtgtg
      781 gccctcgggc gacttccggg ctgaccccac ggacccggcc acggcgcacc tgttgatcgc
      841 cgtggtcgcc ctcgccgccc tggtcggcat cggcctggcc gtgcgcgcgc gggcgtggtc
      901 gctcctcctc tacgtcgggg cggcggtggt cgccggcctg gcgatcttcg tgatcggctc
      961 gccgtgggtg ggcggcaagg cgctggccac ggcctcgccg gccttgcccc tggccgccgt
     1021 ggcggcgggc gccgcactgt acgcggaagg gcggcgggtc gaggggggca tcctgatggc
     1081 ggtgctggcg gccggaatcc tgtggtcgaa cgccctcgcc taccgcgacg tcaacctggc
     1141 gccccgcgat cagctcgccg agctccagga gatcggcgag ctgatcgcgg gccagggccc
     1201 gacgctgatg acggagtacc agccgtacgg cgtcaggcac ttcttgcgca acgccgatcc
     1261 cgaggcagcc tcggagctgc gccgccggcg cgtaccgctg ctcagcggca ggaccctgcg
     1321 caagggggcc tacgccgaca ccgaccggtt caagctcgac ggcctcgagg tctaccggac
     1381 gctcgtcgtg cgccgctccc cggcgcagag ccgcccgccg ttgccgtacc ggctgatctg
     1441 gcgtggccac tactacgagg cctggcagcg ccccgccggc tcggagggcg tcgtccgcga
     1501 ccacctcggt ctcggcaacg aggtcgaccc tggcggggtt cccaagtgcg ccgacgtgct
     1561 ccatctcgcc gacgaggcgg gcccgaacgg cagactggcc gccgtcggcc gggcccccgt
     1621 ggtcatcgtc tcgctgaagc agaccagcca cccgcgcgcc tggaactcgc ctgactatgc
     1681 gtcggcgttg ctgccgacca cccccggggc gatcaaggcg cagatccgcg tcagcaagcc
     1741 cggcggctac gagatctggc tcggcggctc ggtgcggccg caggtcgacg tcgccgtgga
     1801 cggacgcccg gtgggctcgg ttcgggagca gctcgagaac tccggccagt acgtctcgct
     1861 ggggacggcc cagctcgacc ccggcgagca caccgtgacg atccgctttc atggcgccga
     1921 cctccatccg gggagcggcg gcgcgcccga tccgatcggc cccctggtgc tgagccagca
     1981 ggacatggcc gacacccgcg tcgtctactt cccgcccagc gaggcgaccc ggctgtgcgg
     2041 caagcgctgg gactggatcg aggcgctcgg ctaggaccca atcgtgcggg caccctagcg
     2101 agcggcgatt agcgccggtc taagtgacgg gcggcgggcg acgaatgagg ggaagttcga
     2161 ccgtcgagcg gccagcttga actcggcgcg ccccggagga gtattacggg caccaatcca
     2221 accaaggaga ccagaacatg atcagagcac ggctcccgct cgcagtcctt cccgccagcc
     2281 tcgtggccct cgtcgcggtg gctggttgcg ggggaggcag cagcagcacc agcacgagcc
     2341 cgagcaccgg cggggcgacg ggtggcggcg cggcgacgat cgacgtcgcc aagaatgcgc
     2401 aacttggcca gatcctcacc gacagcaagg gcaacaccgt ctacgtgttc gctaaggaca
     2461 ccaatgggaa gtcc
//
LOCUS       DASPYY010000587         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_239678, whole genome shotgun sequence.
ACCESSION   DASPYY010000587 DASPYY010000000
VERSION     DASPYY010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239678"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..>1507)
                     /locus_tag="VEP73_09320"
     CDS             complement(314..>1507)
                     /locus_tag="VEP73_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYT26665.1"
                     /translation="GVRVLDLTRLLPGGVLGALLADLGADVVKVEEPGSGDYMRWAEP
                     RIGPESAASWVVGRNKRSLALDLKRPEGVELFLRLAAQAQVLVEGFRPGVVERLGIGY
                     DTVAALNPEIVYASLTGYGSDGPMRDVAGHDINYIAYAGVLGMTGPPGGPVCPPGVQV
                     GDLGGASLLGVGLLAALYAAERFGRGSHVEVAMYDAALAWTSIHAGETWATGRAPQPG
                     RALLNGGMPCYNVYACADGRYLSVGALEPKFWTVFCTAVDRPDLVGRAFDPDARVEVA
                     AVVAGRTRDEWVGLLEPLEACVAPVLDMREALAHPLAAARSMVLDAPLDPGGNATAPT
                     LGTPIRFDGRPRGPGAAPPALGADSVALAAQAGLSGREIAALLESGVLQQAASGLVAP
                     DAGTG"
BASE COUNT          217 a          586 c          525 g          179 t
ORIGIN      
        1 tgcggtcgac ccgcacggtc ggcccggtga cgtcggggcc tgccgaggcc gcagacccgg
       61 agacgagcag cgccgcgaca accgtgaccg ctgcgcagac ggccacggac tgtgctgcgg
      121 acgcccgccg acccgtacga atgcgtgcca ggaccatgcc gcgtccttcc tctcgcgtgt
      181 tgctgcgcgg tgctgcgcgc gtcgccggcc cgatgctggt ccagcccgca ccaggatgca
      241 agcacctgga tgcacgcacg ccagcaccgg aatgccagcg cgtacgaacg ccggaggtca
      301 tccgacggtt cggtcagccc gtcccggcgt cgggcgccac caagccggaa gccgcctgtt
      361 gcaggacgcc ggactcgagc agtgccgcga tctcccggcc gctgaggccg gcctgtgccg
      421 cgagcgccac cgagtccgcc cccagcgccg gcggcgcggc cccgggccca cgcgggcggc
      481 cgtcgaagcg gatcggcgtg ccgagcgtcg gcgccgtcgc gtttccgccc gggtcgagcg
      541 gtgcgtccag gaccatcgac cgggcggcag ccagcgggtg cgcgagcgcc tcgcgcatgt
      601 cgagcaccgg agccacacac gcctcgagcg gctccagcaa cccgacccac tcgtctcggg
      661 tgcggccggc gaccacggct gctacctcga cgcgggcgtc ggggtcgaaa gcccgcccga
      721 ccaggtccgg gcggtcgacc gcggtgcaga agaccgtcca gaacttgggt tcgagggcgc
      781 ccaccgacag gtaccgcccg tccgcgcagg cgtacacgtt gtagcagggc atgcccccgt
      841 tcagcagcgc ccgtccgggc tgtggcgcac ggcccgtggc ccaggtctcg ccggcgtgga
      901 ttgacgtcca cgccagcgcc gcgtcgtaca tcgcgacctc gacgtgcgaa ccccggccga
      961 agcgctcggc cgcgtagagc gcggccagca gcccgacgcc cagcagcgac gcgccgccga
     1021 ggtcgccgac ctgcactccc ggcgggcaga ccggtccccc tggtgggccc gtcatgccga
     1081 gcacgcctgc gtacgcgatg tagttgatgt cgtgcccggc gacgtcgcgc atcggaccgt
     1141 ccgagccgta gccggtcagc gaggcgtaca cgatctcggg attgagcgcg gcgaccgtgt
     1201 cgtagccgat ccccagccgc tcgaccacgc ctggccggaa accctcgacg aggacctgcg
     1261 cctgcgccgc cagccgcagg aacagctcga cgccctcggg ccgcttcagg tcgagcgcga
     1321 gcgagcgctt gttccgtccg accacccagg aggctgcgga ctccggcccg atccgtggct
     1381 cggcccagcg catgtagtcg ccggaacctg gctcttcgac cttcacgacg tcagcaccga
     1441 ggtcggcgag cagcgcgccg agcacgccgc ccggcaacag cctggtcagg tcgagcaccc
     1501 gcacccc
//
LOCUS       DASPYY010000588         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1505068, whole genome shotgun sequence.
ACCESSION   DASPYY010000588 DASPYY010000000
VERSION     DASPYY010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505068"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VEP73_09325"
     CDS             complement(<1..1064)
                     /locus_tag="VEP73_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYT26666.1"
                     /translation="MRSEPVEPGGPGGPGPGGPGWPRRLHPLSPVFLSVREGRRLWPL
                     GLFLLAQRRLWLIGVAVAVVVAVAVLSWLRRTYVLEVGALRVEQGVLARRRRVVPFDR
                     IQQVDLVRKPLHRLLGVATLRVETAGGPRASEVDLDVIALAEAERLRAALLRARAVTA
                     GLPGAVDGPVPGGAGTPSGPEASPRPERTLVRLTLGQVALAGVTGSRAAAVLALLSPV
                     FQVVDQVPRWLVDRLGPEVLAPSSPLAVAALVPVAALVWLGLAAGSSIVTDAGFVLAR
                     SGDNLVVRRGLLERREAVVPLGRIQVVRIEESLLRRSLGYAAVRIQSAGSAGRSDHQA
                     SRLAVPLLPAVRVNQLLDELL"
     gene            complement(1054..1503)
                     /locus_tag="VEP73_09330"
     CDS             complement(1054..1503)
                     /locus_tag="VEP73_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYT26667.1"
                     /translation="MLLVWSLEEAVGSAVLVALVVAADVGARVAGAEPPWPPGAAAAP
                     FVLLGALAAWRIPLARFRCWRYELAEDALELRHGIVERIHSAIPYYRVQYVDIVQGPV
                     ERMVGLATLKVHTAAASSDAAIPGVPAAEAESLRRLILARTGTGDAV"
     gene            1660..>2495
                     /gene="ispH"
                     /locus_tag="VEP73_09335"
     CDS             1660..>2495
                     /gene="ispH"
                     /locus_tag="VEP73_09335"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYT26668.1"
                     /translation="MASDAMQSDAIPDFQAPATVLLAAPRGYCAGVDRAVQIVQVALR
                     TYGPPVYVRKQIVHNLHVVRGLEGEGAVFVGELHEVPEGATVVFSAHGVAPEVHAEAR
                     ARGLRVVDATCPLVTKVHQEARKFAASGYDIVLVGHEGHEEVVGTMGHAPLAVHLVGD
                     ADEARAVEVPDPGKVAYLTQTTLSVDETTGIVGALRDRFPALAGPRSDDICYATQNRQ
                     TAVKALAAECDLILVIGAGNSSNSKRLVEVARDHGCASHLIPDASAVDPAWFEGVGTV
                     GVT"
BASE COUNT          357 a          981 c          912 g          245 t
ORIGIN      
        1 aggagctcgt cgaggagctg gttcacccgc accgcgggca ggagcgggac cgcgagccgg
       61 ctcgcctggt gatccgacct gccggcgctc cccgcactct ggatgcggac cgcggcgtag
      121 ccgagggacc ggcgcagcag cgactcctcg atgcggacga cctggatccg cccgagcggg
      181 accaccgcct cgcggcgctc gagaaggccg cgccggacga ccaggttgtc gccgctcctc
      241 gccagcacga agccggcgtc ggtgacgatg ctcgacccgg cggccaggcc gagccacacc
      301 agcgcagcca cgggcacgag cgcggccacc gccagggggc tcgacggcgc cagcacctcc
      361 ggccccagcc ggtcgacgag ccaccggggc acctggtcga cgacctggaa caccggggag
      421 aggagcgcga gcaccgcagc ggcgcgggag ccggtcaccc cggccagcgc cacctgcccg
      481 agcgtcaggc ggacgagcgt ccgctctggc cgcggggacg cctcgggacc gctcggcgtc
      541 cccgccccgc cgggaacggg gccgtccacg gcgcccggga ggcccgcggt cacggcgcgc
      601 gcccgcagca gggcggcgcg caggcgctcg gcctcggcca gcgcgatcac gtcgaggtcc
      661 acctcggagg ctctcggacc ccccgccgtc tcgacccgca gggtggccac cccgaggagg
      721 cggtggagcg gcttgcggac caggtcgacc tgctggatgc ggtcgaacgg gaccacccgt
      781 cgccggcggg cgagcacccc ctgctccacg cggagcgcgc ccacctccag gacgtaggtc
      841 cggcgcagcc acgagagcac ggccaccgcg accacgaccg ccacggccac cccgatcagc
      901 cagagccgcc gctgggcgag gaggaagagc ccgagcggcc agagccgccg cccctcccgg
      961 accgagagga acaccgggct gagcgggtgg agccttctcg gccaccccgg cccgcccggc
     1021 cccggcccgc ccgggccgcc gggctccacc ggctcagacc gcatcgccgg tgccggtccg
     1081 ggccaggatc agccgccgca gcgactccgc ctcggcggcg ggcacgccgg ggatggccgc
     1141 gtcgctcgag gccgccgcgg tgtgcacctt gagggtggcc agccccacca tccgctcgac
     1201 cggcccttgg acgatgtcga cgtactggac ccggtagtag gggatcgccg agtggatccg
     1261 ctcgacgatc ccgtgccgca gctcgagcgc gtcctcggcc agctcgtagc gccagcaccg
     1321 gaacctggcg agcgggatcc gccaggcggc gagcgccccg aggaggacga acggggcggc
     1381 ggcggcgccc ggcggccagg gcggctcggc acccgcgacg cgggcgccga cgtccgccgc
     1441 gacgacgagg gccacgagca cggcggaccc gaccgcctcc tccaggctcc agaccagcag
     1501 cacgcggcgg tcgagctgcc gcgcccccga caggccaccg gggacgccgg acgctccggc
     1561 cagacggcac ctcccctcgc ccggcgcgac ccgctcgcgc cgccatggaa aggctagccg
     1621 agcgccggcc ggtagaatgg gcccatgtcg aacgacgcga tggcaagtga cgcgatgcag
     1681 agtgacgcga tccccgactt ccaggccccg gccacggtgc tcctggccgc gccgcgtgga
     1741 tactgcgccg gcgtcgaccg cgccgtccag atcgtccagg tcgcgctgcg cacctacggt
     1801 ccgccggtct acgtccgcaa gcagatcgtc cacaacctcc acgtagtccg cgggctcgag
     1861 ggcgagggcg ccgtgttcgt cggcgagctg cacgaggtgc ccgagggcgc gacggtggtg
     1921 ttctccgccc acggcgtcgc ccccgaggtg cacgccgagg ccagggcgag gggcctgcgg
     1981 gtggtcgacg cgacctgccc gctggtgacc aaggtccacc aggaggcgcg caagttcgcg
     2041 gccagcggct acgacatcgt gctcgtcggg cacgagggcc acgaggaggt cgtcggcacc
     2101 atgggccacg cgccgctggc cgtccacctc gtcggcgacg ccgacgaggc ccgcgcggtc
     2161 gaggtgcccg acccgggaaa ggtggcctac ctcacccaga ccaccctgtc ggtcgacgag
     2221 acgaccggga tcgtcggcgc cctccgcgac cgcttccccg cgctcgccgg gccccggtcg
     2281 gacgacatct gctacgccac gcagaaccgg cagaccgcgg tcaaggcgct cgccgccgag
     2341 tgcgacctga tcctcgtgat cggcgccggg aactcctcca actccaaacg gctggtcgag
     2401 gtggcccgcg accacggctg cgcgagccac ctgatcccgg atgcctcggc cgtcgacccg
     2461 gcgtggttcg agggcgtcgg gacggtcggg gtcac
//
LOCUS       DASPYY010000589         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_662351, whole genome shotgun sequence.
ACCESSION   DASPYY010000589 DASPYY010000000
VERSION     DASPYY010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662351"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VEP73_09340"
     CDS             complement(<1..1215)
                     /locus_tag="VEP73_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HYT26669.1"
                     /translation="MTPRPAPPSPSRARSPGAGACRLLAEPLASRLGPAEVALLFAAD
                     PRPFALVGAWAGGGALVGSEPLRVDQGRDPFAAVDRLPSVAVTPAPPPGAVGGGWFGY
                     LGYQLGARVERLPAPPARPVPLPPAVLGFYDHLLRYDQTTGQWWFESLWTPGRSGALA
                     EKLARCRARLAGPPPPVPAWRAGRFTSTPDRARHLSAVAACRAHIAAGDVFQANVCLR
                     LEAAFEGAPAGMLAHALGRLVPPRAAWLEGPWGAVVSLSPELYLRRQGRQVVTAPIKG
                     TCPRDPDPAAAARQRDGLVASAKDRAENVMIVDLMRNDLARVCRPGTVDVPALVRAEP
                     HPGIWHLVSRVHGILRDGVRDSDLLRATFPPGSVTGAPKPRAMELIHELESTAREVYT
                     GAIGYASPLAGLE"
     gene            complement(1285..2307)
                     /locus_tag="VEP73_09345"
     CDS             complement(1285..2307)
                     /locus_tag="VEP73_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT26670.1"
                     /translation="MEYRTLGSTGTVVSTLCLGTMTFGSESDEKVAHEQLDRFVERGG
                     NFLDTADVYAGGESEHVIGRWLAARPGMRDRVVVATKGRFDRGGGQNDVGLSRVSLTR
                     ALDASLRRLGVDVIDLYQAHAWDPLTPIEETLRFFDDTVRAGKVQYVGVSNFIGWQLQ
                     KAALLARHLGLAPIVTLQPQYNLLQRGVELELSQVCVNERIGILPWSPLGGGWLSGKY
                     QRDTAPTGATRLGENPERGMEAYARRNREERTWRVVDALRSAAEARGLSMAQVALAWL
                     VDRPAVSSVILGARTVEQLDENLGAAGLHLSAEETARLDEVSTPVVDDYPYGAAGVSQ
                     RDRMLA"
     gene            2474..3115
                     /locus_tag="VEP73_09350"
     CDS             2474..3115
                     /locus_tag="VEP73_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT26671.1"
                     /translation="MRLTKFGHSCLLVEEGRARMLFDPGVFSDGFEGLEGLTAVLYTH
                     QHADHIDPERLRGLLDRNPAARVVSDEGTARLLGEAGAEVEVVGEGHEFEIGGVGVRV
                     AGREHAVIHPDIPVVPNVGYLVGERLFHPGDAFTLPGRPVEVLAVPAGAPWLKVSEAI
                     DYLRAVGPKVAVPVHEKVLSTVGISLHYRQLEQLGARKGTALRVMDDGKPVEL"
     gene            complement(3168..3875)
                     /locus_tag="VEP73_09355"
     CDS             complement(3168..3875)
                     /locus_tag="VEP73_09355"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HYT26672.1"
                     /translation="MSEKTPGALRQLHGGLVVSCQAPPGDPLHGPTFMAAMARAAVLG
                     GAVGIRANGLSDIEAVRRAVDVPVIGLWKDGTQEVYITPTVRHAHAVVEAGAQIVAID
                     ATTRPRPDGSTVPEVIEELRGTNCLVMADVSTLAEGVLAARSGADLVATTLSGYTPYS
                     PQRSTPDLELVTALAARVDVPVVAEGRIGTPRQAGDALDAGAWAVVVGGAITRPQHIA
                     ERFVAALRTRADEAAAP"
     gene            complement(3872..>4059)
                     /locus_tag="VEP73_09360"
     CDS             complement(3872..>4059)
                     /locus_tag="VEP73_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT26673.1"
                     /translation="VVGGGVAQPAGRYWSAAERHYREHLLPAAREVPLLPALLGKDAV
                     LVGAAELARRGISERTP"
BASE COUNT          530 a         1476 c         1545 g          508 t
ORIGIN      
        1 ctccagcccg gccagcgggc tggcgtaccc gatggcgccg gtgtagacct cccgcgcggt
       61 cgactcgagc tcgtggatca gctccatcgc gcggggcttg ggcgcgccgg tcacgctgcc
      121 gggcgggaac gtggcacgca gcaggtcgct gtcacggacg ccgtcgcgca ggatcccgtg
      181 gacccggctg accaggtgcc agatgcccgg gtgcggctcg gcgcgcacca gggcgggcac
      241 gtcgaccgtg ccgggccggc agacacgggc gaggtcgttg cgcatcaggt cgacgatcat
      301 cacgttctcg gcccggtcct tggccgacgc gaccagcccg tcgcgctgcc tggctgcggc
      361 ggcggggtcg gggtcgcgcg ggcaggtccc cttgatcggc gcggtgacca cctgccggcc
      421 ctggcggcgc aggtacagct cgggcgacag gctcaccacc gcgccccagg ggccctcgag
      481 ccacgccgcg cggggtggga cgagccgccc gagcgcgtgg gccagcatgc cagccggcgc
      541 gccctcgaac gcggcttcga ggcgcaggca cacgttggcc tggaagacgt ccccggcggc
      601 gatgtgcgcc cggcaggccg ccaccgcgga caggtgccta gcgcggtcgg gtgtgctggt
      661 gaacctgcct gcccgccagg ccggcacggg cgggggcggg ccggccaggc gtgcgcggca
      721 gcgcgccagc ttctcggcca gggcgcccga gcgccccggg gtccacagcg actcgaacca
      781 ccactgcccc gtggtctggt cgtagcgcag caggtggtcg tagaagccaa gcaccgccgg
      841 gggcagcggc accggcctgg ctggcggcgc gggcagccgc tccacgcgcg cgccgagctg
      901 gtagccgagg tacccgaacc agccgccgcc gaccgctcca gggggtggcg cgggcgtcac
      961 ggccacgctg gggagccggt cgacggcggc gaacgggtcg cggccctggt cgacccgcag
     1021 cggctcggag ccgaccagcg cgccgccgcc cgcccacgcg ccgaccagcg cgaacgggcg
     1081 cgggtcggcg gcgaacagca gggcgacctc ggcggggccg agcctcgacg ccagcggctc
     1141 ggccagcagc cggcacgcac cggcgcccgg cgaccgggcc cgggacgggg acggaggcgc
     1201 aggccgaggc gtcatgggtt caccccgctc gtcggtctcg cctccctggc ccggggcaga
     1261 gcgtgagccc aaaccccggt gctgtcacgc aagcatgcgg tcgcgctggc tcaccccggc
     1321 ggcgccgtac gggtagtcgt ccacgaccgg cgtgctgacc tcgtccagcc tggccgtctc
     1381 ctcggccgac aggtgcaggc cggccgcgcc caggttctcg tcgagctgct cgaccgtgcg
     1441 cgcaccgagg atgaccgagg acaccgccgg acggtccacc agccaggcca gggccacctg
     1501 cgccatcgag agaccgcgcg cctcggcggc cgaccgcagg gcgtcgacga cccgccaggt
     1561 gcgctcctcc cggttgcgtc gcgcgtaggc ctccatgccg cgctcgggat tctcgcccag
     1621 acgggtcgcg ccggtcggtg ccgtgtcgcg ctggtacttg ccggagagcc agccgccccc
     1681 gagcggcgac cacggcagga tgccgatgcg ctcgttgaca cagacctggc tgagctcgag
     1741 ctcgacgccg cgctgcagca ggttgtactg cggctggagc gtcacgatcg gtgcgaggcc
     1801 gaggtgccgg gccagcagcg ccgccttctg cagctgccac ccgatgaagt tgctgacccc
     1861 gacgtactgg accttgcccg cacgcaccgt gtcgtcgaag aaccgcagcg tctcctcgat
     1921 cggggtcaac gggtcccagg cgtgcgcctg gtacaggtcg atgacgtcga cgccgagccg
     1981 ccggaggctc gcgtccaacg cccgggtcag gctgacccgg gagagcccga cgtcgttctg
     2041 cccgccgccc ctgtcgaagc ggcccttggt ggcgaccacc acccggtcgc gcatccccgg
     2101 ccgggccgcg agccaccgcc cgatgacgtg ctccgactcc ccgcccgcgt agacgtcggc
     2161 ggtgtcgagg aagttgccac cccgctccac gaaccggtcg agctgctcat gggcgacctt
     2221 ctcgtcgctc tcgctcccga acgtcatcgt gcccaggcag agggtggaca cgaccgtgcc
     2281 ggtgctcccg agcgttcggt actccatcgg cgcatgcctc cgtcgagtcg accgccgcgg
     2341 ctccagcggc ggggtgatgc cggacagcgt gccacgatct tggcgcccag aggccaacgc
     2401 cgaccgccgg gggctggcgc cccggccggc gtggtaggac atgggggacg agcggcgagc
     2461 tcaaggaggc gtcatgcggc tgaccaagtt cgggcactcg tgcctgctgg tggaggaagg
     2521 gcgggccagg atgctgttcg acccgggcgt gttctccgac gggttcgagg ggctggaggg
     2581 gctgacggcg gtgctgtaca cccaccagca cgccgaccat atcgacccgg agcggctccg
     2641 cgggctgctg gaccgcaacc cggcggcccg ggtcgtcagc gacgagggaa ccgcccggct
     2701 ccttggcgag gccggggcgg aggtcgaggt cgtgggcgag gggcacgagt tcgagatcgg
     2761 cggggtgggc gtgcgcgtcg ccgggcgcga gcacgcggtc atccatccgg acatcccggt
     2821 ggtccccaac gtcgggtacc tggtcggcga gcggctgttc cacccgggcg acgcgttcac
     2881 cctgccaggg cggccggtcg aggtgctcgc ggtgccggcc ggggcaccct ggctgaaggt
     2941 gtcggaggcg atcgactacc tgcgcgccgt gggcccgaag gtggccgtcc cggtgcacga
     3001 gaaggtcctc tcgacggtcg ggatatcgct tcactaccgg cagctcgagc agttgggagc
     3061 gaggaagggc acggcgctcc gggtgatgga cgacgggaag ccggtggagc tctagcgtcg
     3121 ccgccgcctg cccgagctcg ccgacccagg ggcacgcgag cgtgccttca cggggcggcc
     3181 gcctcgtccg cgcgggtccg cagagcggcg acgaaccgct cggcgatgtg ctgcggtcgt
     3241 gtgatcgcgc cgccgacgac gacggcccat gctccggcgt cgagggcgtc gccggcctgc
     3301 cgcggcgtgc cgatccgccc ctcggcaacc accgggacgt cgacgcgtgc agccagcgcc
     3361 gtgaccagct ccagatcggg ggtggaccgc tgcgggctgt agggggtgta gccggacagg
     3421 gtggtcgcga ccaggtcggc gcctgacctc gcggcgagga ccccctcggc gagggtggac
     3481 acgtcggcca tgaccaggca gttggtcccc cggagctctt cgatgacctc cgggacggtg
     3541 gatccgtcgg gccgtggacg ggtcgtcgcg tcgatggcga cgatctgcgc ccccgcctcg
     3601 acgacggcat gcgcgtgccg gacggtgggc gtgatgtaca cctcttgggt gccgtccttc
     3661 cacagtccga tgaccggcac gtcgacggcg cgccgcacgg cttcgatgtc ggacagcccg
     3721 ttggcgcgga tcccgaccgc accaccgagg acggctgcgc gagccatcgc cgccatgaag
     3781 gtcgggccgt ggagcggatc acccggcggc gcctggcagg acacgaccag acctccatgg
     3841 agctgccgca gggcgcccgg ggtcttctcg ctcatggtgt ccgctccgag atgccgcggc
     3901 gcgccagctc ggcggcgccg acgagaaccg cgtccttgcc gagcagtgcc ggcagcaggg
     3961 ggacctcgcg tgcggccggc agcagatgct cgcggtagtg ccgctcggcc gcggaccagt
     4021 accggccggc cggctgcgcg acgccgccgc cgacgacga
//
LOCUS       DASPYY010000590         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_662476, whole genome shotgun sequence.
ACCESSION   DASPYY010000590 DASPYY010000000
VERSION     DASPYY010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662476"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VEP73_09365"
     CDS             <1..176
                     /locus_tag="VEP73_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT26674.1"
                     /translation="FFLDRYTDAYRRELDHFITAVEEGTKPEPGFADGRAALVLADAA
                     AQSLRTNSTVRLA"
     gene            208..1236
                     /locus_tag="VEP73_09370"
     CDS             208..1236
                     /locus_tag="VEP73_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT26675.1"
                     /translation="MSKVRWGLLSTASIGRVVTAATRQAQHAEFVAVASRDAARARQF
                     ADELGLAASFGSYEELLASDAVDAVYVALPVSMHTEWTIKSLEAGKHVLCEKPFAIGP
                     EDAARCFDAASSAGRLSAEGLMYRHHPQTALARKLVADGTIGPLAHVRAALSVSVAKG
                     DIRRSVELGGGALLDLGCYCVSAIRLFADEPERVYAEQVRDGTDGVDLRFAATARMRH
                     DVQAQFDTGLDLPRRDELELIGTDGRLTIPDPWLCRSGFIELERDGATERLPVDPAGA
                     FGLTQDERDAYRIEFDAVSAAITGGPQSSFGREDAIDQATVLQALRRSSEQRSPIELP
                     APRQLPAT"
     gene            1409..>1758
                     /locus_tag="VEP73_09375"
     CDS             1409..>1758
                     /locus_tag="VEP73_09375"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HYT26676.1"
                     /translation="MDDPPQALLDRMAAAADDWLGSLGPGQLAKARYDFPAPEERTRW
                     FYVPVEQGGLPLAEMGPVQQRLAHRLLASGLSRPGYVAAATVMGLENVLDAIEEWQLP
                     YPGRAVPNRGRDPLL"
BASE COUNT          242 a          642 c          639 g          235 t
ORIGIN      
        1 acttcttcct cgaccgctac accgacgcct accgccgaga gctcgaccac ttcatcaccg
       61 cggtcgagga ggggaccaag ccggagcccg ggttcgccga cgggcgcgca gcgctcgtgc
      121 tcgccgacgc cgccgcccag agcctgcgga ccaactcgac cgtccggctc gcttgaggcc
      181 ggcctcgacg cgcagaggag cggcaagatg agcaaggtcc gttgggggct gctctccacg
      241 gccagcatcg gccgggtcgt caccgcggcg acccgccagg cacagcacgc cgagttcgtc
      301 gccgtcgcga gccgggatgc cgcacgggcc cggcagttcg ccgacgagct cgggctcgcg
      361 gcgagcttcg gctcctacga ggagctcctc gcctccgacg cggtcgacgc ggtctacgtg
      421 gcgctcccgg tctcgatgca caccgagtgg accatcaagt cgctcgaagc aggcaagcac
      481 gtcctgtgcg agaagccgtt cgccatcggc cctgaggatg ccgcccgttg cttcgacgcg
      541 gcgtcgtcgg cgggcaggct gtccgccgag gggttgatgt accgccatca cccgcagacc
      601 gcgctggccc ggaagctggt cgccgacggg acgatcggac cgctggcgca cgtgcgtgcc
      661 gccttgagcg tcagcgtggc caagggcgat atccggcgtt cggtggagct cggcggcggg
      721 gcgctgctcg atctgggctg ctattgcgtg agcgccatcc ggctgttcgc cgacgagccg
      781 gagcgggtct acgccgagca ggtgcgcgac gggaccgatg gggtcgacct ccgcttcgcg
      841 gcgaccgcga ggatgcgcca cgacgtgcag gcccagttcg acacgggcct cgaccttccg
      901 cggcgggacg agctcgagct gatcggcacc gacggcaggc tcacgatccc tgacccgtgg
      961 ttgtgccggt ctgggttcat cgagctggag cgcgacggcg cgaccgagcg cctgccggtc
     1021 gatccggccg gcgcgttcgg cctcacccag gatgagcgcg acgcctaccg catcgagttc
     1081 gacgcggtct cggccgcgat caccggcgga ccgcaatcgt cgttcggacg cgaggacgcg
     1141 atcgaccagg cgacggtgct ccaagccctc cgtcgatcca gcgagcaacg atccccgatc
     1201 gagctgccgg ccccgcggca gctcccggcg acctgacggg agggagcttc gggtgggtgc
     1261 tccgctccga ggtggtgggc tcgccgaccc gacgccagac cggctcgagc tcggccgtgc
     1321 gccgccgcgc tccccaagga gcgggtgcac ctcgacgccc aggcgctgct cgcccgggac
     1381 gacatcgacg agcttcggga ggactgccat ggacgaccca ccccaggccc tgctggaccg
     1441 gatggcggcg gcggcggacg actggctggg gtcgctcggg ccggggcagc tggccaaggc
     1501 ccgctacgac ttcccggccc cggaggagcg gacccgctgg ttctacgtgc cggtcgagca
     1561 gggtggcctg ccgctggccg agatgggccc ggtccagcag cgcctcgccc accggctgct
     1621 ggccagcggc ctgagccggc ccggctacgt ggccgccgcg accgtcatgg gcctggagaa
     1681 cgtgctcgac gcgatcgagg agtggcagct gccgtacccg ggccgggccg tccccaaccg
     1741 cgggcgcgac cccctgct
//
LOCUS       DASPYY010000591         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2346829, whole genome shotgun sequence.
ACCESSION   DASPYY010000591 DASPYY010000000
VERSION     DASPYY010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346829"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..851
                     /locus_tag="VEP73_09380"
     CDS             126..851
                     /locus_tag="VEP73_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYT26677.1"
                     /translation="MSARLLGIDSGLTVTKAVVFDPGGRILGTGEVPSEQRHPRQGWV
                     EKDPDDQWKGACRAVAKALGRAGVEGPDVGAVGVTGHGDGLYVLDGERRPARPAILSL
                     DGRAGAVVERFRRDGVSDRALELTGEAPFAVQPPVLLAWVKQHEPEVYRRIRHVLFCK
                     DWLKLQLTGRVTTDPTEASAGFTDARTQRYAPEALPLYGLEELGDALPEVVGCAEAAG
                     EVTAEAAEATGCFAATGSSPGSG"
     gene            848..1492
                     /locus_tag="VEP73_09385"
     CDS             848..1492
                     /locus_tag="VEP73_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HYT26678.1"
                     /translation="MNMANSAASATNLEWFVRRLAPREADEARARGGSPFAFAGEEVA
                     SVLDDDSRVLYLPFLYGSLDNPDATGVFFGLRAWHTRGHLLRALFEGVAFNHRTHVDA
                     LRDHFEVTEARLTGGGARSQAWGQVFADVLGLPVGVPDAAEAGALGVALCAAVAAGVY
                     SSVAEGADATVRVARTFEPDPRRQEHLAGVYQRYLALVEALGPSWARAAGQEAT"
     gene            complement(1504..>2031)
                     /locus_tag="VEP73_09390"
     CDS             complement(1504..>2031)
                     /locus_tag="VEP73_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYT26679.1"
                     /translation="PADFAAGPDRPGVAYADLRAGVPAWLPEVVRHAGAGADVVLVTP
                     HWGPNMTAAPVGHVRRAAAALVAAGAALVAGHSAHVPHGVAGRVLYDLGDFLDDYRVD
                     ARLRNDLGLLFLVTLDGRGPVRLEALPLKLDFCRTGLASGKDAAWLRRRFRAACAALG
                     TEVDEAAGRLLVHWR"
BASE COUNT          244 a          794 c          770 g          223 t
ORIGIN      
        1 ccccacgtcg ccgggtccac cgaggaggca tggaggaagt cggtcgacct ggtcgccgag
       61 gcgctccggg agctccatgc gaccggccgg gccgcgaaca ccgtcaaccc gggcgcgggc
      121 gagcggtgag cgctcgcctg ctcggcatcg actccggcct gaccgtgacc aaggccgtgg
      181 tcttcgaccc tggcggccgc atcctcggca ccggcgaggt cccgagcgag cagcgccacc
      241 cccgccaggg ctgggtcgag aaggaccccg acgaccagtg gaagggggcc tgccgcgccg
      301 tcgccaaggc cctcggccgg gccggggtcg aggggccgga cgtcggcgcg gtcggcgtga
      361 cgggccacgg cgacggcctc tacgtcctcg acggggagcg ccgcccggcc cgtccggcga
      421 tcctctccct ggacggccgg gccggggcgg tggtcgagcg gttccggcgc gacggggtga
      481 gcgaccgcgc cctcgagctg accggcgagg cgccgttcgc ggtgcagcca ccggtgctgc
      541 tggcatgggt gaagcagcac gagcccgagg tctaccggcg catccgccac gtgctcttct
      601 gcaaggactg gctcaagctc cagctgaccg gccgggtcac caccgacccg accgaggcca
      661 gcgccggctt caccgacgcg cgcacgcagc gctacgcgcc cgaggcgctg cccctgtacg
      721 ggctcgagga gctcggcgac gccctgcccg aggtcgtcgg ctgcgccgag gcggccgggg
      781 aggtgacggc cgaggcggcc gaggccaccg ggtgctttgc cgcaactggg tcgagcccgg
      841 gcagtggatg aacatggcca actcggccgc ctcggccacc aacctcgagt ggttcgtgcg
      901 ccgcctggcc ccccgcgagg cggacgaggc gcgggcccgc ggcgggtcgc cgttcgcgtt
      961 cgccggcgag gaggtcgcgt ccgtcctcga cgacgacagc cgcgtcctct acctgccctt
     1021 cctgtacggc tcgctggaca acccggacgc caccggcgtc ttcttcgggc tcagggcctg
     1081 gcacacccgc ggccacctgc tccgcgccct gttcgagggg gtggccttca accaccgcac
     1141 gcacgtcgac gccctgcggg accacttcga ggtgaccgag gcccgcctga ccggcggcgg
     1201 cgcccgcagc caggcgtggg ggcaggtctt cgccgacgtc ctcggcctgc cggtgggcgt
     1261 gcccgacgcc gcggaggcgg gcgcgctcgg cgtggcgctc tgcgccgccg tggccgccgg
     1321 cgtctactcc tcggtggccg agggggccga cgccacagtg cgggtcgccc gcaccttcga
     1381 gcccgacccg cgccgccagg agcacctggc cggcgtctac cagcgctacc tggcgctggt
     1441 cgaggcgctc ggcccctcct gggcccgcgc cgccggtcag gaggcgacct gaccggccac
     1501 ggctcagcgc cagtggacca gcagccggcc ggccgcctcg tccacctcgg tgccgagcgc
     1561 cgcgcaggcc gcccggaagc ggcgccgcag ccaggccgcg tccttccccg acgcgaggcc
     1621 ggtgcggcag aagtcgagct tgagcggcag cgcctccagc cgcaccgggc cgcgcccgtc
     1681 gagggtgacc aggaacagca gccccaggtc gttgcgcagc cgcgcgtcga cccggtagtc
     1741 gtcgaggaag tcgccgaggt cgtacaggac gcggccggcc accccgtggg gcacgtgggc
     1801 ggagtggccg gcgaccaggg ccgcgcccgc cgccaccagc gccgccgcgg cgcggcgcac
     1861 gtgcccgacc ggcgcggccg tcatgttcgg cccccagtgg ggcgtcacca gcaccacgtc
     1921 cgcgcccgcg cccgcatggc gcacgacctc gggcagccag gcgggcaccc ccgctcgcag
     1981 gtcggcgtag gcgaccccgg gccggtcggg gccggcggcg aagtcggccg g
//
LOCUS       DASPYY010000592         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1364000, whole genome shotgun sequence.
ACCESSION   DASPYY010000592 DASPYY010000000
VERSION     DASPYY010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364000"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VEP73_09395"
     CDS             <1..1394
                     /locus_tag="VEP73_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYT26680.1"
                     /translation="ESPPTTETTALARLLSARGLDELRADLELARQRIEGSPDVRARD
                     RAIKRFSALQERFELEGGYRAEAEAKRIAAGLGVPADGLGRPLAGLSGGERRRVELAR
                     VLLGQAGTLLLDEPTNHLDADAKRWLAEFLADFAGGILVVSHDLPLLDRDITAVLALD
                     PATASVEAYKGTYTRFLEVRKVRAASEARHRKVVERDIKRLSAFVEKYRHSNETMARR
                     ALVTERRVERMRSALAPVKRAGHKVAVRFPDPPPCARFPLEAARLRRSYGGPPVFKEL
                     SFDLERGERLLVLGLNGAGKTTLLRVLAGIDRPDEGEVVLGHGCDIGYYAQEHEGLDP
                     RATSMEVMQAAAKAPDDVLRGVLGHFLLGGDQANQRTASLSGGERTKLALARLVVGRH
                     NVLLLDEPTNNLDVQAVEAVRTALTTFKGSIVLVSHDTPFVQSFEPDRVLVMPEGALD
                     VWSEDYLDLVPLD"
     gene            1474..>2151
                     /locus_tag="VEP73_09400"
     CDS             1474..>2151
                     /locus_tag="VEP73_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HYT26681.1"
                     /translation="MTAPTELFRPVTTRNASKAVVEQVRVAVDLALLVAGDRLPPERE
                     LAALLDVSRPTVREALRMLAESGYVGIRRGASGGAFVLPRHWAGGTERARELLAERRQ
                     ELVALLEWRRAIEAEAAALAAVRVSGDELRSLRARIVESGGWRPRSREAWRAGDSRFH
                     IAVAEASGNPWFVDAVRRARAELAEALDAIVAHQAAEPAAAEHESILAALQARDPGAA
                     RAAALRHG"
BASE COUNT          275 a          788 c          824 g          264 t
ORIGIN      
        1 aggagtcccc gccgaccacc gagacgaccg ccctggcccg gctgctctcc gcgcgcggcc
       61 tcgacgagct gcgggccgac ctggagctgg ccaggcagcg catcgagggg agcccggacg
      121 tgcgggccag ggaccgggcc atcaagcggt tctcggcgct gcaggagcgc ttcgagctgg
      181 agggcggcta ccgggccgag gccgaggcca agcggatcgc ggccggcctc ggcgtcccgg
      241 ccgacggcct ggggcgtcct ctggccggcc tctcgggcgg cgagcggcgc cgcgtcgagc
      301 tcgcccgggt gctgctcggc caggccggga cgctgctgct cgacgagccg accaaccacc
      361 tggacgccga cgccaagcgg tggctggcgg agttcctggc cgacttcgcc ggcggcatcc
      421 tggtcgtctc ccacgacctg ccgctgctgg accgcgacat cacggcggtg ctcgcgctcg
      481 acccggcgac cgcctcggtc gaggcctaca agggcaccta cacgcggttc ctggaggtgc
      541 ggaaggtcag ggccgcctcg gaggcgcggc accgcaaggt cgtcgagcgc gacatcaagc
      601 ggctctcggc cttcgtcgag aagtaccgcc actcgaacga gaccatggca cgccgggcgc
      661 tggtgaccga gcgccgggtc gagcgcatga ggagcgccct cgcgccggtg aagcgggccg
      721 gccacaaggt ggccgtgcgg ttccccgacc cccctccctg cgcccgcttc ccgctcgagg
      781 cggcccgcct gcgccgttcc tacggcggcc cgccggtgtt caaggagctc tcgttcgacc
      841 tcgagcgggg ggagcgcctg ctcgtgctcg ggctcaacgg ggcgggcaag acgacgctgc
      901 tgcgcgtcct ggcaggcatc gaccggcccg acgaggggga ggtcgtcctc gggcacggct
      961 gcgacatcgg ctactacgcc caggagcacg aggggctcga cccgcgggcg acctccatgg
     1021 aggtcatgca ggcggcggcc aaggcccccg acgacgtgct ccgcggcgtc ctcggccact
     1081 tcctgctcgg cggggaccag gccaaccagc ggaccgccag cctctccggc ggcgagagga
     1141 cgaagctcgc gctcgcccgc ctggtcgtcg gccgccacaa cgtcctgctc ctcgacgagc
     1201 cgaccaacaa cctcgacgtg caggcggtcg aggcggtgcg gacagccctc acgacgttca
     1261 aggggagcat cgtgctggtc agccacgaca cgcccttcgt gcagagcttc gagcccgacc
     1321 gggtcctggt gatgcccgag ggcgcgctcg acgtgtggag cgaggactac ctggacctgg
     1381 tcccgctgga ctgaccggcg acccggaggt gacgcggcgg gcaccggaaa ggtaaccttg
     1441 taagaccttt cgatgacgtg ttggagcgtg cccgtgacgg cgccgaccga gctgttccgg
     1501 ccggtcacca cccgcaacgc ctcgaaggcc gtggtcgagc aggtcagggt ggcggtcgac
     1561 ctcgccctcc tggtcgccgg cgaccgcctg ccgcccgagc gggagctcgc ggccctgctc
     1621 gacgtgagcc gcccgacggt ccgggaggcg ctccgcatgc tcgcggagtc cggctacgtc
     1681 gggatccgcc ggggcgcgag cgggggggcg ttcgtcctgc cgaggcactg ggccggcggc
     1741 accgagcggg cacgggagct gctcgccgag cggcgccagg agctggtggc gctgctcgag
     1801 tggcggcgcg cgatcgaggc cgaggcggcg gcgctcgccg cggtccgcgt ctccggcgac
     1861 gagctgcgct cgttgcgggc gcggatcgtc gagtcgggcg gatggcggcc gcgcagcagg
     1921 gaggcgtggc gggcgggtga cagccgcttc cacatcgcgg tcgccgaggc gtcgggcaac
     1981 ccctggttcg tggacgccgt ccggcgggcc cgggccgagc tcgccgaggc gctcgacgcg
     2041 atcgtcgccc atcaggcggc ggagccggcg gcggccgagc acgagtcgat cctcgccgcc
     2101 ctgcaggcgc gcgaccccgg cgccgcccgg gccgcggccc tgcgccacgg t
//
LOCUS       DASPYY010000593         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1644875, whole genome shotgun sequence.
ACCESSION   DASPYY010000593 DASPYY010000000
VERSION     DASPYY010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644875"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1812
                     /locus_tag="VEP73_09405"
     CDS             88..1812
                     /locus_tag="VEP73_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT26682.1"
                     /translation="MPTRAGSGVPTGTGTAEPGGPIGTGGHDRRLAGDEDPDVSFGVA
                     EFLAAEEMGRHRGYWWSPDGERLAVARVDEGPVQRWWLADPASPGVAPARLAYPAAGT
                     ANADVRFFVVDLAGERVEVAWDRAAFPYLVEVVWGADGPLTLLVQARDQTRARVLAAD
                     PETGATSCVREDTDPAWLEIVPGVPAWLPGGRLVWTVDDRASDTRRLVLDQEAVTPPG
                     LQVREVLGTRGDTVLVVASEEPTEQHVWEVPAGGTPKRLTTTPGVHTAVTSRTGQGEG
                     PVTVLLSTSLDADGTQATVHAAGRPAVTVGSLAEVPASRPRVTLLRAGARSLRTALLL
                     PGGTEPDGPLPVLLDPYGGPHAQRVVAAAGAYLTSQWFADQGFAVVVTDGRGTPGRGP
                     AWERAVRGDLAGPVLDDQVDALHAVAALDPRLDLGRVAIRGWSFGGWLAALAVLRRPD
                     VFHAAVAGAPATDWLLYDTHYTERYLGHPAAEPDAYRRHSLLGSAAGLERPLLLIHGL
                     ADDNVVAAHTLRLSAALLVAGRPHTVLPLTGVTHMASQEVVAENLLHFQLSFLRAALG
                     LPHGGNGG"
BASE COUNT          213 a          744 c          736 g          231 t
ORIGIN      
        1 gaggcgcgcc ttggcgtgcc gagcgggaca gggcaagccg tgccgaacgg gacaggcgaa
       61 ggcgtgccga ccgggaaaga gcaaggcgtg ccgaccaggg ccggcagcgg cgtgccgacc
      121 gggacaggga cagccgagcc cggcgggccg atcgggaccg gcggccacga ccgccgcctc
      181 gcgggcgacg aggacccgga cgtctccttc ggcgtggccg agttcctcgc cgccgaggag
      241 atgggccgcc accgcgggta ctggtggtcg cctgacgggg agcgcctggc ggtggcgcgg
      301 gtggacgagg ggccggtgca gcgctggtgg ctcgccgacc cggcgagccc gggggtcgcg
      361 cccgcccggc tggcctaccc ggccgccggg accgccaacg ccgacgtgcg gttcttcgtc
      421 gtcgaccttg ccggcgagcg ggtggaggtg gcgtgggacc gggccgcgtt cccctacctc
      481 gtcgaggtcg tctggggcgc cgacgggccg ctcaccctcc tcgtccaggc gcgcgaccag
      541 accagggcga gggtcctcgc cgccgacccg gagaccggcg cgacgagctg cgtgcgggag
      601 gacaccgacc cggcctggct cgagatcgtc cccggcgtgc cggcctggct gccaggcggc
      661 cggctcgtgt ggacggtcga cgaccgggcc tccgacaccc gccggctcgt tctcgaccag
      721 gaggcggtga ccccgcccgg gttgcaggtc cgcgaggtcc tcggcacgcg gggcgacacc
      781 gtgctcgtcg tcgcgtccga ggagccgacc gagcagcacg tctgggaggt cccggccggc
      841 ggcaccccga agcggctcac caccaccccc ggggtgcaca cggcggtcac cagccgaacg
      901 ggacagggag aggggccggt gaccgtgctc ttgtcgacca gcctggacgc cgacgggacg
      961 caggcgaccg tgcatgccgc cggccggccg gccgtgacgg tcggctcgct cgctgaggtc
     1021 ccggcgtcga ggccccgggt gaccctcctg cgggccggcg cgcggtcgct gaggacggcg
     1081 ctgctcctgc ctggcggcac cgagcccgac gggcccctgc cggtcctgct cgacccgtac
     1141 ggcggccccc acgcccagcg ggtggtggcg gccgccggcg cctacctcac ctcgcagtgg
     1201 tttgcggacc agggcttcgc cgtggtggtg acggacggcc gcggcacgcc ggggcggggc
     1261 ccggcgtggg agcgggccgt ccgcggcgac ctcgccggcc cggtgctcga cgaccaggtg
     1321 gacgccttgc acgcggtggc cgcgctggac ccgcggctcg acctcggccg ggtcgcgatc
     1381 cgcggctggt cgttcggcgg ctggctcgcc gctctggccg tgctgcgccg gccggacgtg
     1441 ttccacgcgg ccgtcgccgg cgcgccggcc accgactggc tgctgtacga cacccactac
     1501 accgagcgct acctcggcca cccggccgcc gagccggacg cgtaccggcg ccattccctg
     1561 ctcggctccg ccgccggcct cgagcggccg ctactgctca tccacggcct cgccgacgac
     1621 aacgtggtcg ccgcgcacac gctgcgcctg tcggccgccc tgctcgtcgc gggccggccg
     1681 cacaccgtcc tgccgctgac cggggtcacc cacatggcgt cacaggaggt cgtggccgag
     1741 aacctgctgc acttccagct ctcgttcctg cgggccgcgc tcgggctgcc gcacggcggc
     1801 aacggtggct gacggaccgc ccgtcgaggg caccttctct atcccggtcg gcacggctct
     1861 cccggtcccg gtcggcacgg cttgccctgt cccgctcggc acgccaaggc gcgcctccga
     1921 accg
//
LOCUS       DASPYY010000594         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_240623, whole genome shotgun sequence.
ACCESSION   DASPYY010000594 DASPYY010000000
VERSION     DASPYY010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240623"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEP73_09410"
     CDS             complement(<1..191)
                     /locus_tag="VEP73_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26683.1"
                     /translation="MSSGGEGPARARPGPGTPPRFGVPVAYPPGGPRRDGRRPGGGRR
                     HVEGRALAGWGRRAAATLL"
     gene            complement(342..1226)
                     /locus_tag="VEP73_09415"
     CDS             complement(342..1226)
                     /locus_tag="VEP73_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYT26684.1"
                     /translation="MAAGLLRDGLTVGVGVASGMLSAVAGVGGAVVSTPGIRALGASA
                     FVAVGTTLPSILPGAASGTLRYRREGLVDWRLVGWAAPSGAAATVAGSLLSHAVPGQG
                     HLLMLATAALLALTAVRTVRHRGGPGPGPAPVPASGVPGSPVPASGVPASGAGRDGQP
                     GPGRRASFRRAAWPAAVAVGAGAGLLSGLLGVGGGIVLVPGFGELLGLPIKAAIATSL
                     ACVGILAIPGTVTHALLGDIDWRFAALLALGVVPGARLGAAVAVRAAERPLRLGLGAL
                     LGLIAVWYMASELRALVR"
     gene            complement(1236..2639)
                     /locus_tag="VEP73_09420"
     CDS             complement(1236..2639)
                     /locus_tag="VEP73_09420"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYT26685.1"
                     /translation="MADIDAFVKAEAESLVAELSRWCAIPSISTDPSHADDVRRSAEH
                     LAGLMEAAGLHAEVIPVEGGQPAVYAEWLGAGPEAPTITVYGHHDVQPVDPLEQWTSP
                     PFEPAVRDGCLHARGASDDKGQVHFQVSAIRHLLAADGSLPVNVKFFVEGEEEAGSPN
                     VEAFLADHAERLACDVVLVSDTGMHSPEVPSMVTSMRGLMYFQVDLRTATADLHSGTF
                     GGTVPNAVEALVRLLSGLKDGAGRIAIPGWYDDVLDLTDEERRQFAALPFDPEELKRD
                     AAGVRVLPGEEGWSPLERIGGRPAADVNGIWGGYTGVGSKTIIPAEAHAKVSFRLVAD
                     QEPAKLEPLFEQWVRDGAPEGGEVVIQSFGKVRPCLTPLDHPGNRAAARAVARAFGAE
                     PLFTREGGSGPERALSDALGVSCVYVGVMLPSDRTHAPDERLHLSHYYRGVRAAAYAY
                     EEFARPGVVDALRALRG"
     gene            2860..>3044
                     /locus_tag="VEP73_09425"
     CDS             2860..>3044
                     /locus_tag="VEP73_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010278263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYT26686.1"
                     /translation="MAGSMTSIRSNGGSVPAYVSLPPSGSGPGVVVIQEWWGLVDHIK
                     DVADRFAAEGFVALAPDL"
BASE COUNT          396 a         1147 c         1186 g          315 t
ORIGIN      
        1 tcgagcaggg tcgcggcggc gcggcggccc cagccggcca gggctcggcc ctcgacgtgg
       61 cggcggccgc cacccggtcg ccggccgtcg cggcgggggc cgcccggcgg gtaggcgacc
      121 gggacgccga accgtggcgg cgtgcccggc ccggggcgcg cccgagccgg gccttctcct
      181 ccagacgaca tccggccccc tcacgtgccc gctcccagcc gctcgcgtgg cgccggggga
      241 gcctccccga tgcgtcgagg tcgccgcaat cgggatctct acgcagcgca cgagggcgac
      301 ccgggcgccc gccgccctgg ctcgctggac gccgccgcgg ctcagcggac aagggcgcgc
      361 agctcgcttg ccatgtacca gacggcgatc aggccgagca gggcgccgag ccccagccgc
      421 aggggccgct cggcggccct gacggcgacc gcggcgccaa ggcgggcgcc cgggacgacg
      481 ccgagggcga gcagggcggc gaacctccag tcgatgtcgc cgagcagcgc gtgggtgacg
      541 gtgccgggga tggcgaggat gccgacgcag gccagcgagg tggcgatcgc cgccttgatg
      601 gggaggccga gcagctcgcc gaacccgggg acgaggacga tcccgccgcc taccccgagc
      661 agtcccgaga gcaggcccgc ccccgccccg acggccaccg cggccggcca ggccgcgcgc
      721 cggaatgagg cccggcgccc ggggcccggc tggccgtccc gcccggcccc ggacgccggg
      781 accccggacg ccgggaccgg ggaccccggg accccggacg ccgggaccgg ggcgggaccg
      841 gggccggggc cgccccggtg gcggacggtc cggaccgcgg tcagggccag cagcgccgcg
      901 gtcgccagca tgagcaggtg gccctggccg gggaccgcgt gcgagagcag ggagccggcg
      961 accgtggcgg ccgcgccgga gggtgccgcc cacccgacca ggcgccagtc gacgagcccc
     1021 tcgcgccggt agcgcagggt gccgctggcc gcccccggca ggatggaggg cagggtcgtg
     1081 ccgaccgcga cgaacgcgga agcgccgagc gcccggatgc caggggtcga gaccaccgcc
     1141 ccgccgaccc cggccacggc gctgagcatc ccgctggcca cgccgacgcc gaccgtcagc
     1201 ccgtcccgca gcaacccggc ggccatgccc ggccgtcagc cgcgcagtgc ccgcagggcg
     1261 tcgaccacgc caggccgggc gaactcctcg taggcgtacg cggcggcccg caccccgcgg
     1321 tagtagtgcg agaggtggag ccgctcgtcg ggcgcgtggg ttcggtcgga gggcagcatg
     1381 accccgacgt agacgcagga gacgccgagc gcgtcggaga gggcacgctc agggcccgag
     1441 ccaccctccc gggtgaacag cggctcggcg ccgaaggcgc gggcgaccgc ccgggccgcg
     1501 gcccggttgc ccgggtggtc gagcggggtg aggcagggcc tcaccttccc gaacgactgg
     1561 atgacgacct cgccgccctc gggcgcgccg tcgcgcaccc actgctcgaa caacggctcc
     1621 agcttggctg gctcctggtc ggcgaccagc cggaacgaca ccttcgcgtg cgcctcggcc
     1681 gggatgatgg tcttcgaccc gaccccggtg tacccgcccc agatgccgtt gacgtcggcc
     1741 gccgggcggc cgccgatgcg ctcgagcggc gaccagccct cctcgccggg caggacccgc
     1801 acgccggccg cgtcgcgctt cagctcctcg gggtcgaagg gcagggccgc gaactgccgg
     1861 cgctcctcgt cggtcaggtc gaggacgtcg tcgtaccagc cggggatggc gatccggccg
     1921 gcgccgtcct tgagccccga gaggagccgg acgagcgcct ccaccgcgtt cggcaccgtg
     1981 cccccgaacg tgcccgagtg caggtcggcc gttgccgtgc ggaggtccac ctggaagtac
     2041 atgagcccgc gcatcgaggt gaccatcgaa ggcacctcgg gcgagtgcat gccggtgtcg
     2101 gacacgagca cgacgtcgca ggccagccgc tcggcgtggt cggcgaggaa cgcctcgacg
     2161 ttcggcgagc ccgcctcctc ctcgccctcg acgaagaact tgacgttgac gggcaggctc
     2221 ccgtcggccg cgagcaggtg gcggatggcc gacacctgga agtggacctg ccccttgtcg
     2281 tcggaggcgc cccgggcgtg caggcagccg tcgcgcacgg ccggttcgaa cggcggcgag
     2341 gtccactgct cgagcgggtc gacgggctgg acgtcgtggt ggccgtagac ggtgatggtc
     2401 ggggcctcgg gacccgcgcc gagccactcg gcgtagacgg cgggctggcc tccctcgacc
     2461 gggatgacct cggcgtggag gccggccgcc tccatcaggc cggccaggtg ctcggcggag
     2521 cggcggacgt cgtccgcgtg ggaggggtcg gtcgagatcg acgggatggc gcaccatctg
     2581 gacagctcgg cgacgagcga ctccgcctcg gccttcacga aagcgtcgat gtcagccacg
     2641 ggctccctcg cggaacgggg tgaaacggca tccgcaggct accagagggg gccggcgagc
     2701 tcgcccggtg gccccgggcg gccctggacc gacggttccc ggcggcccgg gccgatggtt
     2761 gcccggcccc gggcgtggaa ggatgcgccg gggcgcgggc ctcccgcgcc gggcgcccgg
     2821 cggcgccgac ggaccgactc ggcacacagg aggaaccaca tggccggatc catgaccagc
     2881 atcaggtcga acggcgggtc ggtccccgcc tacgtctccc tgcccccgtc gggcagcggg
     2941 cccggggtcg tcgtcatcca ggagtggtgg gggctcgtcg accacatcaa ggatgtcgcc
     3001 gaccgcttcg ccgctgaggg cttcgtcgcg cttgcgccgg acct
//
LOCUS       DASPYY010000595         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_663446, whole genome shotgun sequence.
ACCESSION   DASPYY010000595 DASPYY010000000
VERSION     DASPYY010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663446"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VEP73_09430"
     CDS             complement(<1..1476)
                     /locus_tag="VEP73_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26687.1"
                     /translation="MVQQEGMELPEVVGVLVSNEMMEILLATEAPAAPEPFVLSYSRR
                     RWGLPVEVPLERLREAAGDAVSPVPALVTLGRTSNGLLLVNLESPGLTALGGDEDAAR
                     AVLTSFAVELATCRWADYIELVLVGFGGELQRLERARTVASVREMLPWLEHKADDVAD
                     LLGETGARSVLAGRIAGTTPDGWVPAVVLCAERPTAEEVTRLVALTAVPSRSPVAAVI
                     AAGDVSGARWTLDLPEAASEPLHLPQFGVDVVPQRLDPDDYDGIARLLETTTRDDVPI
                     PDPMREPARRPFRRPRPAPVPIDDDYDDKLDEDGIETETDEGSDVTSTEPAEHDEVAV
                     PTAAAASSEPAESEPAESADGAPATGEPEPEPGAEAEPRKTPHLHELRLSSRVIPDPE
                     MNDEPEVEVRVLGKVEIGGIERVERGKSEELIVFLALHPDGVDGDQLSEALWPGRPPA
                     KGTLNTTTAVARAYLGMTPDGEPRLPHARNGIYRLDPSVGLD"
BASE COUNT          178 a          560 c          563 g          213 t
ORIGIN      
        1 gtccagcccc accgacgggt ccagccggta gatgccgttg cgggcgtggg gcaggcgcgg
       61 ctcgccgtcg ggggtcatgc cgaggtaggc gcgggccacc gcggtggtgg tgttgagggt
      121 gcccttggcc ggcgggcggc cgggccacag cgcctcggag agctggtcgc cgtccacgcc
      181 atccgggtgg agcgccagga acacgatcag ctcctcggac ttgccgcgct cgacccgctc
      241 gatgccgccg atctccacct tgccgagcac gcgcacctcg acctcgggct cgtcgttcat
      301 ctccgggtcg ggaatcaccc ggctgctgag gcgcagctcg tgcaggtgcg gcgtcttgcg
      361 cggctcggcc tcggctccgg gctccggctc cggctcgccg gtcgcgggcg cgccgtctgc
      421 gctctctgcg ggctcgctct ctgcgggctc gctgctcgcg gcggccgccg tgggcacggc
      481 aacctcgtcg tgctctgcgg gctctgtgct ggtcacatca gacccttcat cggtctcggt
      541 ctcgatgccg tcctcatcga gcttgtcgtc gtagtcgtcg tcgatgggaa ctggcgctgg
      601 ccgtggccgg cggaagggcc ggcgggccgg ctcccgcatg ggatccggga tggggacgtc
      661 gtcccgcgtg gtggtctcga gcaggcgcgc gatgccgtcg tagtcgtccg ggtcgagccg
      721 ctgcggcacc acgtcgacgc cgaactgggg caggtggagc ggctcgctgg cagcctcggg
      781 aaggtccagg gtccagcggg ctccggagac gtccccggcg gcgatcaccg cggcgaccgg
      841 tgagcgggag ggcacggcgg tgagcgcgac cagccgggtg acctcctcgg cggtgggccg
      901 ttccgcgcat agcaccaccg ccgggaccca gccgtccggg gtggtgccgg cgatgcggcc
      961 ggccagcacc gagcgggcgc cggtctcgcc gagcaggtcg gcgacgtcgt cggccttgtg
     1021 ctccagccac ggcagcatct cccggacgct ggcgacggtg cgggcccgct ccagccgctg
     1081 cagctcgccg ccgaagccga ccaggaccag ctcgatgtag tcggcccagc ggcaggtggc
     1141 cagctccacc gcgaacgagg tgagcaccgc cctggccgcg tcctcgtcgc cgccgagggc
     1201 ggtcaggccg ggagactcca ggttgaccag cagcagcccg ttgctggtcc gcccgagcgt
     1261 caccagcgcc ggcaccggcg agacagcgtc gccagccgcc tcccggagcc gctccagcgg
     1321 cacctcgacc ggcaggcccc agcggcggcg ggagtacgac agcacgaacg gctcgggcgc
     1381 ggccggcgcc tcggtggcca gcaggatctc catcatctcg ttgctgacca gcacgccgac
     1441 gacctcgggc agctccatgc cctcctgctg caccaccgcc gccatcgtcc gcaggcccag
     1501 gtcgaccagc tcgg
//
LOCUS       DASPYY010000596         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2628240, whole genome shotgun sequence.
ACCESSION   DASPYY010000596 DASPYY010000000
VERSION     DASPYY010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628240"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..928)
                     /locus_tag="VEP73_09435"
     CDS             complement(89..928)
                     /locus_tag="VEP73_09435"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HYT26688.1"
                     /translation="MRRTRQRWHPETLVCAFRGHETPAAGVAVLRPRDGGLGLDLPDG
                     RRFARCIRCDAWVALQPPERPHGEHLPPHGDIELPRRGKALRDLIVLRLIAVDRGLHA
                     IVFALVAVGLVVLRYKLPLVQAEAQDVLRGLGRAVVSPTGDAPGRDLLVRGLERVLRL
                     QAKSLKTLLLIAVAYAVVEGVEAVGLWWQRRWAEYLTVIATAGLIPFEVEEIVRRVTV
                     VRVGALVVNLAIVVYLVWAKRLFGLRGGAHADEEALEAARLLAPPPERDHGGRAPTPT
                     RPA"
     gene            complement(959..1243)
                     /locus_tag="VEP73_09440"
     CDS             complement(959..1243)
                     /locus_tag="VEP73_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HYT26689.1"
                     /translation="MTAAGTGQPRTVRHLVHCSLPPELVHQPILHGLVQATRAVVNVR
                     RANVDVEAGIAWFILELSGPVDEVEAAERWLVAAGVAVEPIEGGDPERAD"
     gene            complement(1240..2058)
                     /gene="larE"
                     /locus_tag="VEP73_09445"
     CDS             complement(1240..2058)
                     /gene="larE"
                     /locus_tag="VEP73_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HYT26690.1"
                     /translation="MDELDTKRAQLRKELQASGGVMVAFSGGADSAFLLAAAVEALGG
                     RAVAATGVSPSLPEHELADARALADELGARHVVVATQELDRPGYVANGADRCYFCKSE
                     LFDLLVPLAQIEGLALAVGTNTDDLGDWRPGQLAAAEHGVLAPLVDAGFAKADVREAS
                     RRMGLRTAEKPAAACLASRLAYGIEVTPVRLSRVERAEAVLRSYGLDRLRVRDHGDLA
                     RVEVPLEALPMLVEPDRRAHLVARLRDLGFAFVTLDLEGFRSGSMNASLGIEAR"
     gene            complement(2168..3787)
                     /gene="groL"
                     /locus_tag="VEP73_09450"
     CDS             complement(2168..3787)
                     /gene="groL"
                     /locus_tag="VEP73_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYT26691.1"
                     /translation="MPKIIAFEEDARRSLERGMNQLADAVRVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDIAGDGTTTATVLAQALVREG
                     LRNVAAGANPMSLKRGIEKAVERAVEAVKHQAKEVETREEIAHTATISAGDPEIGEVI
                     AKAMDVVGKEGVVTVEESNTFGIELETVEGMRFDKGYVSAYFVTDPERMEAVFDDPYV
                     LIANQKISAVKDLLPVLEKVMQTGKPLVIIAEDVDGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLGDIAVLTGGEVISEEVGLKLENVTLDLLGRARKVVVTKDETTIIEGQ
                     GDQEQIKARISQIKQEIERTDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKEKKH
                     RIEDAVSNARAAVEEGIVPGGGVALLRAQEALTKVDLTGDDATGAAIVRRALEEPLKQ
                     IAVNAGLEGGVVVEKVRGLADAHGLDAATGEYVDMFKAGIIDPAKVTRSALQNAASIA
                     ALFLTTEAVVADKPEKEKAPAMPGGGGDMDF"
     gene            complement(3900..>4199)
                     /locus_tag="VEP73_09455"
     CDS             complement(3900..>4199)
                     /locus_tag="VEP73_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26692.1"
                     /translation="RRARALHELLAGLGRSERAGRLDELRASLAELVADLESVGAPAA
                     ELRPLRELLAALEGPASGGVEAPGAGPSPAGEELDRLWDRALAVLDGFARPGRGR"
BASE COUNT          522 a         1534 c         1536 g          607 t
ORIGIN      
        1 cctcgacctg tcggccgccg acgcggtcgc cgacgacctg gccggcgcgg tcggctggat
       61 cctggcctga cgggcggcag gccgccgcct acgcgggccg ggtgggcgtg ggggcgcggc
      121 cgccgtggtc ccgctcgggc gggggggcga gcagccgggc cgcctcgagc gcctcctcgt
      181 cggcgtgcgc gccgccccgc agcccgaaga gccgcttcgc ccagaccagg tagacgacga
      241 tcgccaggtt gacgaccagg gcaccgaccc gcacgacggt cacccgcctg acgatctcct
      301 cgacctcgaa ggggatcagc ccggcggtgg cgatcaccgt gaggtactcg gcccagcggc
      361 gctgccacca gaggccgacg gcctcgaccc cctcgacgac cgcataggcg acggcgatga
      421 ggagcagcgt cttcaggctc ttggcctgca gccgcaggac gcgctcgagg ccgcggacca
      481 ggaggtcgcg ccccggggcg tcccccgtcg gggagaccac ggcccggccg agccctcgca
      541 ggacgtcctg ggcctcggcc tggaccagcg gaagcttgta gcggagcacg acgagaccga
      601 cggcgacgag ggcgaacacg atggcgtgga ggccccggtc gaccgcgatg agccgcagca
      661 cgatcaggtc gcgcagggcc ttgccgcggc gcgggagctc gatgtcaccg tgcgggggca
      721 ggtgctcgcc gtggggacgc tcgggtggct gcagggccac ccacgcgtcg cagcggatgc
      781 agcgcgcgaa gcgccgcccg tcgggcaggt cgagcccgag cccgccgtcc cgcgggcgga
      841 gcacggccac gccggcggcc ggcgtctcgt gaccgcggaa cgcgcagacc agcgtctccg
      901 ggtgccagcg ctggcgggtg cgcctcatca cggtgcccgc ggagcacggc gggaccagct
      961 agtcggcacg ttcggggtcg cccccctcga tcggctcgac ggcgaccccg gcggccacca
     1021 gccagcgctc ggccgcctcg acctcgtcca cgggcccgga gagctcgagg atgaaccagg
     1081 cgatcccggc ttcgacgtcc acgttggccc ggcgcacgtt gaccaccgcg cgggtggcct
     1141 ggacgaggcc gtgcaggatg ggctggtgca ccagctcggg cggcagcgag cagtggacga
     1201 ggtggcggac ggtccgcggc tgtcccgtcc ccgccgcggt catcgcgcct cgatcccaag
     1261 gctcgcgttc atgctgccgg agcggaaccc ctcgaggtcg agggtgacga acgcgaagcc
     1321 gaggtcacgc agccgggcga cgaggtgcgc ccgacggtcg ggctcgacga gcatgggcag
     1381 cgcctcgagg ggcacctcga ccctggcgag gtcgccgtgg tcgcggaccc gcagccggtc
     1441 gaggccgtag gagcgcagga cggcctcggc ccgctcgacc cgggacagcc ggacgggggt
     1501 cacctcgatc ccgtaggcca gccgggaggc gaggcaggcg gcggccggct tctcggcggt
     1561 ccgtaggccc atccggcggg acgcctcgcg gacgtcggcc tttgcgaacc cggcgtccac
     1621 gagcggcgcg agcaccccgt gctcggcggc cgcgagctgg cccggacgcc agtcgccgag
     1681 gtcgtcggtg ttggtgccga cggcgagggc gagcccttcg atctgggcga gaggcacgag
     1741 aaggtcgaac agctcggact tgcagaagta gcagcggtcg gcgccgttgg cgacgtagcc
     1801 gggccggtcc agctcctggg tggccacgac gacgtgcctg gccccgagct cgtccgcgag
     1861 cgcgcgggca tcggccagct cgtgctctgg caggctcggg gagacgccgg tcgcggccac
     1921 ggcgcgcccg ccgagcgcct cgacggccgc cgcgagcagg aaggccgagt cggctccgcc
     1981 ggagaacgcg accatcacgc cgccgctcgc ctgaagctcc ttgcgcagct gcgcccgctt
     2041 ggtgtccagc tcgtccattg ccgttcccgt cgccgccagg tcgtccgggc gccattctac
     2101 gggcgggacg gcgacgggcg ggcccgtggg gcccgcccgt cgtgaagtcg cgatctggag
     2161 accggactca gaagtccatg tcgccgccgc cgcccggcat ggccggggcc ttctccttct
     2221 ccggcttgtc ggcaaccacc gcctcggtgg tgaggaacag ggccgcgatc gacgcggcgt
     2281 tctgcagcgc cgagcgggtc accttggccg ggtcgatgat gccggccttg aacatgtcga
     2341 cgtactcgcc ggtggccgcg tcgagaccgt gggcgtccgc gaggccgcgc accttctcga
     2401 ccacgacgcc gccctccagg ccggcgttga cggcgatctg cttcagcggc tcctcgagcg
     2461 cgcggcgcac gatcgcggcg ccggtcgcgt cgtcaccggt gaggtcgacc ttggtcagcg
     2521 cctcctgcgc cctgagcagg gccacgccgc cgccggggac gatgccctcc tcgaccgccg
     2581 cgcgggcgtt cgagacggcg tcctcgatgc ggtgcttctt ctccttgagc tcgacctcgg
     2641 tggccgcgcc gaccttgatg acggcgaccc cgccggccag cttcgccagc cgctcctgca
     2701 gcttctcgcg gtcgtagtcg gagtcggtcc gctcgatctc ctgcttgatc tggctgatgc
     2761 gggccttgat ctgctcctgg tcgccctggc cctcgatgat cgtggtctcg tccttggtga
     2821 cgaccacctt gcgggcccgg ccgagcaggt cgagggtgac gttctcgagc ttgaggccga
     2881 cctcctcgct gatgacctcg ccgccggtga ggaccgcgat gtcgcccagc atggccttgc
     2941 ggcggtcgcc gaacccgggg gccttgaccg cgaccgactt gaacgtcccg cggatcttgt
     3001 tgaccaccag ggtggccagc gcctcgccgt cgacgtcctc ggcgatgatc accagcggct
     3061 tgccggtctg catgaccttc tcgaggaccg ggagcaggtc cttgacggcc gagatcttct
     3121 ggttggcgat gaggacgtag gggtcgtcga agaccgcctc catgcgctcg gggtcggtca
     3181 cgaagtaggc cgagacgtag cccttgtcga accgcatgcc ctcgaccgtc tcgagctcga
     3241 tgccgaaggt gttcgactcc tcgacggtga cgacgccctc cttgccgacc acgtccatcg
     3301 ccttcgcgat gacctcgccg atctcggggt cgccggccga gatggtcgcc gtgtgggcga
     3361 tctcctcgcg ggtctcgacc tccttggcct ggtgcttgac ggcctcgacc gcccgctcga
     3421 cggccttctc gatgccgcgc ttcagcgaca tggggttggc gccggcggcg acgttgcgca
     3481 gcccctcgcg gaccagcgcc tgcgcgagca cggtggcggt ggtggtgccg tcaccggcga
     3541 tgtcgtcggt cttcttggcg acctccttga cgagctcggc cccgatcttc tcgtaggggt
     3601 cctcgagctc gatctccttg gcgatggaga cgccgtcgtt ggtgatcgtg ggcgcgcccc
     3661 acttcttctc gagaacgacg ttccggcccc gcggcccgag cgtcacccgt acggcgtccg
     3721 cgagctggtt catgccccgc tcgagtgacc gcctggcgtc ctcctcgaag gcgatgatct
     3781 tgggcatgcg ctggctacct ccctctaggt ggcttgcctt gctaggtggc ttgccttggc
     3841 atccgggcct gacggccgga tgtgctggcg cggatgttag cactcatgta ctgcgagtgc
     3901 taacggccgc ggcccggccg cgcgaacccg tccagcaccg cgagcgcccg gtcccagagc
     3961 cggtccagct cctcgccggc gggcgacggt cctgcgccgg gcgcctcgac cccgccggag
     4021 gcgggtccct cgagcgcggc gagcagctcg cgcaggggcc gcagctcggc cgctggcgcg
     4081 ccgaccgact cgaggtcggc gaccagctcg gccaggctgg cacggagctc gtccagccgc
     4141 cctgcccgct cggacctccc caggccggcc agcagctcgt gcagggcccg ggcgcggcg
//
LOCUS       DASPYY010000597         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1225983, whole genome shotgun sequence.
ACCESSION   DASPYY010000597 DASPYY010000000
VERSION     DASPYY010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225983"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VEP73_09460"
     CDS             complement(<1..1005)
                     /locus_tag="VEP73_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYT26693.1"
                     /translation="MSPGRRVSRDLVCEELFGDLDPRAAARSVSKALSMARAALAGLG
                     EPGEPAPALLEADLTHIWANPAVEVDAAEHEQALRSALAMGPGHGRDGLLATALADDG
                     ELLADEPYADWALRPRERLEALRQEARLALARDRARGAGDTRPEAQLAAWAACAEHDP
                     ASEEAAAALVRGYFAAGHRELAVRAYERCRAALGELGLGLSPWLEEVYATAAFEAGPP
                     RTQAPAREELRTVSVLAAEVAAAGLADPEELRDVTGRGLAAADRVPQALGIQVADRDH
                     LGGQHAHGPQLLAGGGGRAGSPGPGRHGLEGGGGVHLLEPRGYPDPELAEGGPAALVG
                     A"
     gene            1230..1430
                     /locus_tag="VEP73_09465"
     CDS             1230..1430
                     /locus_tag="VEP73_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26694.1"
                     /translation="MYTGYQIYQAERPKSAREQYEIDKSNARLVRAVGRFGRLLARPV
                     RPRRQTWRPAPGRPAPCPETSQ"
BASE COUNT          182 a          590 c          588 g          182 t
ORIGIN      
        1 cgcgcctacg agcgctgccg ggccgccctc ggcgagctcg gggtcggggt atcctcgtgg
       61 ctcgaggagg tgtacgccac cgccgccttc gaggccgtgc cgtcccggtc cggggctccc
      121 ggcccggccg ccgccccccg cgaggagctg cggaccgtga gcgtgctgtc cgccgaggtg
      181 gtcccggtcg gcgacctgga tcccgagagc ctgcgggacg cggtcggccg cggccaggcc
      241 gcggccggtc acgtcgcgca gctcctcagg gtcggcgagg cccgccgcgg ccacctcggc
      301 ggccagcacg ctgacggtcc tgagctcctc ccgcgccggg gcctgcgtcc ggggcggccc
      361 ggcctcgaag gcggccgtgg cgtacacctc ctccagccac ggggacaggc cgagcccgag
      421 ctcgccgagg gcggcccggc agcgctcgta ggcgcgtacc gcgagctccc ggtgtcccgc
      481 ggcgaagtag ccccggacca gggccgcggc cgcctcctcg gaggcagggt cgtgctcggc
      541 gcaggccgcc caggcggcga gctgggcttc gggccttgtg tcgcctgccc ccctggcccg
      601 gtccctggcc agggccagcc gcgcctcctg gcgcagcgcc tcgagccgct cccgcgggcg
      661 cagcgcccag tcggcgtacg gctcgtcggc gagcagctcg ccgtcgtcgg ccagggcggt
      721 cgcgagcagg ccgtcgcgcc cgtgccccgg tcccatcgcc agcgcggagc gcagagcctg
      781 ctcgtgttcg gccgcgtcga cctcgacggc cgggttcgcc cagatgtggg tcaggtcggc
      841 ctcgagcagg gcgggcgcgg gctcgcctgg ttcgccgagg ccggccagcg cggccctggc
      901 catcgacagc gccttggaga ccgaacgggc ggccgcacgc gggtctagat ccccgaagag
      961 ctcctcgcag acgaggtccc ggctgacccg ccggcccggg ctcaccagga caagctggca
     1021 gagtcgccgg gcgctcggcc gcggccacgg gcccgcggcg cggcccccgg agacgacgga
     1081 gaacggcccg agcagcctga cccgtacggc cacatcccca ttgtcccgca cgtgtcacgc
     1141 gcttttcccg cgccgcgaac cggcgggaac accacgggaa cagtgccttc ctactgttcg
     1201 ggcagaacct tccgaaaggg gccttcgaga tgtacacggg gtatcagatt taccaggccg
     1261 agcggcccaa gtccgctcgc gagcagtacg agatcgacaa gagcaacgcc aggctggtca
     1321 gggcggtcgg acggttcggc cgcctgctgg ccaggccggt gcgtccccgg cgccagacct
     1381 ggcggccggc gccgggacgc ccggccccct gccccgaaac cagccagtag gacgtggccc
     1441 gggggggcca tgggacgcca ggggtgtggc gccgcccggg gtgcggcgct gcccgggccc
     1501 ggggcgaatt tctccgggaa tgccgcgggg cgtgtgggat ta
//
LOCUS       DASPYY010000598         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1365425, whole genome shotgun sequence.
ACCESSION   DASPYY010000598 DASPYY010000000
VERSION     DASPYY010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365425"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1136)
                     /gene="ccsB"
                     /locus_tag="VEP73_09470"
     CDS             complement(138..1136)
                     /gene="ccsB"
                     /locus_tag="VEP73_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HYT26695.1"
                     /translation="MSTTRLAELSDTLAWLAVLSYAVGAVLFGFEFAYRVRWAGLAGL
                     GVAVAGLGANVGAAVTRGLAAHRVPWGNMYEYSVMVGIVTVTTFLVWVARRREVRPLG
                     VFVLLPAVLAIAIGGVALRVPAGPLVPALNSGWIKIHVAAAIVGSSVLCLAAIFSVLY
                     LVKDHFERRAAAMRPPLVAGGARTADRVEVREAIASYVEEEEADGTGTAEARLGGEPA
                     PARTVWDRMPSARRLDDLAYRTTAFGFPIWTFAIIAGAIWAQSAWGRYWGWDPKETWS
                     FITWVIFAAYLHARATAGWRGRGAAWIGVVGLVALLVNFYVVNIWVVGLHSYAGVK"
     gene            complement(1162..>2303)
                     /locus_tag="VEP73_09475"
     CDS             complement(1162..>2303)
                     /locus_tag="VEP73_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HYT26696.1"
                     /translation="EELAAEKGYLREAGSLVFHVSFLLLLVGLAYGKGWGYRGQVTLI
                     EGDSLVNTRVNYDAFTPGRWYGPERLPGFTLHLDDFRNSFWPSGQPREFASYLTAVDA
                     EGRTLRQKVAVNRPMTVDGVRVFQSDYGYAPVVKITVKGRDGRRLTPQDGPLLTLRDP
                     PTEVSNGAVKLPMLDPQVGLDVTMFTGFRLDRGPGGQPEIGNDPRLVNPLLVVFPWRG
                     DLHADRAQSVFTLDRTDLRPLADRPLLIPLGGTRKLPNGLGTVSFTGVRQYTVLTLAR
                     DPGVPVVLAAAVLVLLGLLPSLYVTRRRVWVRVTPMPAGRRVAGCRVEVAGLAMQGKA
                     AFADEFSELAAELGRQLDRSPRSGGGDTLDRVAGEDEAPVRSPSG"
BASE COUNT          350 a          839 c          849 g          265 t
ORIGIN      
        1 ccaggcccgc caggactccg agcgccgcgc cgcacgccgc gccgcacgcg ccgagcggtc
       61 caccggccac cgcgcccgcc cggcccggtg agggttggcc gcccgggaag accccggggt
      121 cgacgccgac cccggggtta cttgacgccg gcgtaggagt gcagcccgac cacccagatg
      181 ttgacgacgt agaagttcac caggagggcg acgaggccca ccaccccgat ccacgccgcg
      241 ccgcgcccgc gccagccggc cgtggccctg gcgtgcaggt aggcggcgaa gatcacccag
      301 gtgatgaacg accaggtctc cttggggtcc cagccccagt accggcccca ggccgactgc
      361 gcccagatcg ccccggcgat gatcgcgaag gtccagatgg ggaagccgaa ggcggtcgtc
      421 cggtaggcga ggtcgtccag ccgcctggcc gacggcatgc ggtcccacac cgtccgggcg
      481 ggggccggct cgccgccgag gcgtgcctcc gctgtccctg tcccgtccgc ttcctcctcc
      541 tcgacgtagc tcgcgatggc ctcccggacc tcgacccggt cggcggtgcg ggcgcccccg
      601 gccacgagcg gcgggcgcat cgcggccgcg cggcgctcga agtggtcctt gacgaggtag
      661 aggacgctga agatggccgc aaggcagagc accgacgacc cgacgatggc cgccgccacg
      721 tggatcttga tccagcccga gttcagggcg ggcacgagcg ggccggcggg cacccggagg
      781 gcgacaccgc cgatggcgat cgcgagcacg gcgggcagga gcacgaagac cccgaggggc
      841 cgtacctcgc gccggcgggc gacccacacg aggaacgtcg tcacggtgac gatcccgacc
      901 atgaccgagt actcgtacat gttgccccat ggcacacggt gggcggcgag cccccgggtg
      961 acggcggcgc ccacgttggc cccgagcccc gccacggcga cccccaggcc ggccaggccg
     1021 gcccagcgca cccggtaggc gaactcgaag ccgaacagca ccgcgccgac cgcgtagctg
     1081 agcacggcca gccatgccag ggtgtcggac agttccgcca gccgcgtggt ggacatcgct
     1141 tctcctctgg gcgtcgggac gctaccccga aggggacctg acgggtgcct cgtcctctcc
     1201 ggcgacccgg tcaagggtat ccccaccgcc ggatcgcggc gaccggtcga gctggcggcc
     1261 gagctcggcc gccagctcgg agaactcgtc ggcgaacgcg gccttgccct gcatggcgag
     1321 cccggccacc tcgacccggc aaccggcgac ccggcgaccg gccggcatgg gggtgacgcg
     1381 cacccagacc cggcggcggg tgacgtagag ggacggcagc aggccgagca ggacgagcac
     1441 ggccgccgcg agcacgaccg gcaccccggg gtcgcgggcc agcgtgagca cggtgtactg
     1501 gcgcacgccg gtgaacgaga ccgtgccgag cccgttcggc agcttcctgg tgccgccaag
     1561 ggggatgagg agcggccggt cggcgagcgg gcgcaggtcg gtgcggtcga gcgtgaagac
     1621 gctctgggca cggtcggcgt gcaggtcgcc ccgccacggg aagaccacga gcagcgggtt
     1681 caccaggcgc gggtcgttgc cgatctccgg ctgccctccc ggcccccggt cgagccggaa
     1741 gccggtgaac atggtcacgt cgagacccac ctgagggtcg agcatgggca gcttgacagc
     1801 cccgttcgac acctcggtgg gcgggtcgcg cagggtgagc aaggggccgt cctgcggggt
     1861 gagccgcctg ccgtcgcgcc ccttgacggt gatcttgacg accggggcgt agccgtagtc
     1921 ggactggaag acccgcacgc cgtccacggt catgggccgg ttgaccgcga ccttctgccg
     1981 cagggtgcgc ccctcggcgt cgacggcggt caggtaggag gcgaactcgc gcggctgccc
     2041 ggacggccag aacgagttcc tgaagtcgtc caggtgaagg gtgaaccccg ggagccgctc
     2101 cggcccgtac cagcggccgg gggtgaaggc gtcgtagttc accctggtgt tcaccaggct
     2161 gtccccttcg atgagggtga cctggccccg gtagccccag cccttgccat aggcgagccc
     2221 gaccagcagg agcaggaagg agacgtggaa gacgaggctg cccgcctccc gcaggtagcc
     2281 cttctcggcg gccagctcct cgg
//
LOCUS       DASPYY010000599         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1928483, whole genome shotgun sequence.
ACCESSION   DASPYY010000599 DASPYY010000000
VERSION     DASPYY010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928483"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2007
                     /locus_tag="VEP73_09480"
     CDS             124..2007
                     /locus_tag="VEP73_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT26697.1"
                     /translation="MTTTAGAPARPAVASTVRRVAALFSPYRAQVAVVGLAILVTSGL
                     GVVNPFLIRAVFDRALFVPGGPRLGLLWALVAAMIAIPVVSGAIAIGQTFLANRMGQR
                     VMEDLRATLYTHLQRMSLRFFTATRTGEIQSRLANDVGGVQSVVTDTAASILANVVIV
                     LSTLVAMVLMSWQLTLLSLCLFPVFVLFTYRVGRRRREVAASTQESKAEMSAITQETL
                     SVSGILLAKVFDRQADEIGRFQRENRRLADLQVRQEMIGRVFFSLVQSFFSITPALVY
                     LVAGLVGPGGRITAGTLVAFTTLQTRLFFPIGQLLQVSVEVQSSLALFQRVFEYLDLP
                     RDIVDTPTARPLGQHDVRGEIRLRHVSFRYEAGAAADGDGARPGRRWALEDLSLEIEP
                     GRLAAIVGPSGAGKTTISYLVPRLYDVTEGSVEIDGTDVREIQLSSLSEIVGMVTQES
                     YLFHASVRGNLLYGRPDATEEEIEAAARAALIHDRVLELADGYDTVVGERGYRLSGGE
                     KQRLAIARVILKDPRILILDEATSALDTTSERLVQAALQPLMAGRTTIAIAHRLSTIL
                     AADVIFVLDRGRLVEQGTHAELVERGGLYARLYAEQYQSGLVEARCQDGLVLSSGQVL
                     TTG"
     gene            2023..2265
                     /locus_tag="VEP73_09485"
     CDS             2023..2265
                     /locus_tag="VEP73_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HYT26698.1"
                     /translation="MDDRDVLGRINELVEEEHRLERQAQGTGLDEAARERLGALEVQL
                     DQCWDLMRQRRARREFGENPDEAHVRASDTVERYLQ"
     gene            complement(2364..>3076)
                     /locus_tag="VEP73_09490"
     CDS             complement(2364..>3076)
                     /locus_tag="VEP73_09490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYT26699.1"
                     /translation="IGKLLRKAATKMAAGEAGTPIVVDAGDVGEYLGRQRFFFEAAER
                     TSVPGVATGLAVTGTGGDVLFVEASAMEGEPGLTLTGQLGDVMKESAAIALSYVRSHA
                     RELGIGPGVEKRRYHVHVPAGAVPKDGPSAGVTMTTALVSLLTDRPVRSEVGMTGEVT
                     LQGRVLPIGGVKQKVLAAHRAGLKEVVLPERNGGDLDDVPESVRAEMTFHLAGDIGQV
                     LEHALGEAADHDTVTEAA"
BASE COUNT          411 a         1118 c         1111 g          436 t
ORIGIN      
        1 cctgcgccgg gtgatcgagg gggcccgcgc cgtgcccggc gacggctgag accgctcgga
       61 gaccggacaa agggatacct tggaggccga gccgggcgac ggaagaggaa gaaggggctg
      121 gtgatgacca cgacggctgg agcgcccgcc cgtcccgccg tggcgtcgac ggtgcggcgg
      181 gtggcggccc tgttcagccc ctaccgggcg caggtggcgg tggtcgggct cgcgatcctc
      241 gtgacctcgg gcctcggggt ggtgaacccg ttcctcatcc gggccgtctt cgaccgggcc
      301 ctgttcgtgc ccggcggtcc ccgcctcggc ctgctctggg cgctcgtcgc ggcgatgatc
      361 gcgatcccgg tcgtgtccgg tgcgatcgcc atcggccaga cgttcctcgc caaccgcatg
      421 ggccagcggg tgatggagga cctgcgcgcc accctctaca cccacctgca gcggatgtcc
      481 ctgcggttct tcaccgccac ccgcacgggc gagatccagt cgcggctcgc caacgacgtc
      541 ggcggcgtgc agtcggtcgt gaccgacacc gcggcgagca tcctcgccaa cgtggtcatc
      601 gtcctctcca cgctggtcgc gatggtgctc atgtcctggc agctcaccct gctgtcgctg
      661 tgcctgttcc cggtgttcgt gctgttcacc taccgggtcg gccggcgccg ccgcgaggtc
      721 gccgcctcca cccaggagtc gaaggccgag atgagcgcga tcacccagga gaccctgtcg
      781 gtctcgggca tcctgctcgc caaggtgttc gaccgccagg ccgacgagat cgggcggttc
      841 cagcgcgaga accggcgcct cgccgacctg caggtccgcc aggagatgat cgggcgggtg
      901 ttcttcagcc tggtccagag cttcttctcg atcacgccgg cgctggtcta cctcgtcgcc
      961 gggctggtcg ggccgggggg ccgcatcacc gcgggcacgc tcgtcgcgtt cacgaccctg
     1021 cagacgcggc tgttcttccc gatcggccag ctcctgcagg tctcggtcga ggtgcagtcg
     1081 tccctcgccc tgttccagcg cgtgttcgag tacctcgacc tgccccgcga catcgtcgac
     1141 acgcccacgg cccgcccgct cggccagcac gacgtgcggg gcgagatccg cctgcgccac
     1201 gtgtcgttcc gctatgaagc cggcgccgcg gcggacggcg acggtgcccg gccgggccgg
     1261 cgctgggccc tcgaggacct gagcctcgag atcgagcccg ggaggctcgc ggccatcgtc
     1321 ggacccagcg gggccggcaa gacgacgatc agctacctcg tgccccgcct ctacgacgtc
     1381 accgaggggt cggtcgagat cgacgggacg gacgtgcgcg agatccagct ctcgtcgctg
     1441 tcggagatcg tcggcatggt cacccaggag agctacctgt tccacgccag cgtgcgcggc
     1501 aacctgctct acggccggcc cgacgccacc gaggaggaga tcgaggcggc ggcgcgggcc
     1561 gcgctgatcc acgaccgcgt gctcgagctg gccgacggct acgacacggt ggtcggcgag
     1621 cgcggctacc gcctgtccgg cggcgagaag cagcgcctcg cgatcgccag ggtgatcctc
     1681 aaggacccgc gcatcctgat cctggacgag gcgacctcgg ccctggacac cacgagcgag
     1741 cggctcgtgc aggccgccct gcagccgctc atggccgggc ggaccacgat cgcgatcgcc
     1801 caccgcctgt cgacgatcct ggccgccgac gtgatcttcg tgctcgaccg cgggcggctc
     1861 gtggagcagg ggacgcacgc cgagctcgtc gagcggggcg gcctgtatgc gaggctgtac
     1921 gccgagcagt accagagcgg cctggtcgag gcccgctgcc aggacggcct ggtgctctcg
     1981 agcggccagg tgctcaccac cggatgaacg agggagaccg acatggacga ccgtgacgtg
     2041 ctcggacgga tcaacgagct ggtcgaggag gagcaccggc tcgagcgcca ggcgcagggc
     2101 accggcctcg acgaggcggc gagggagcgc ctgggcgcgc tcgaggtcca gctcgaccag
     2161 tgctgggacc tgatgcgcca gcgccgggcg cgccgggagt tcggcgagaa cccggatgag
     2221 gcgcacgtcc gcgcctccga caccgtcgag cgctacctgc agtagccacc gcgcggaggg
     2281 agcgcacacg cgaagacggc gggcggggac gcgatctctc gcgctcccgc ccgccgtccc
     2341 gtcaaccgcg gggggctgcc cgcctacgcg gcctcggtca ccgtgtcgtg gtcggcggcc
     2401 tcgccgaggg cgtgctcgag gacctggccg atgtcgccgg cgaggtggaa ggtcatctcg
     2461 gcccgcaccg actccggcac gtcgtcgagg tcgccgccgt tgcgctcggg cagcacgacc
     2521 tccttcaggc cggcccggtg ggcggcgagg accttctgct tcaccccgcc gatcggcagg
     2581 accctgccct gcagggtgac ctcgccggtc atccccacct cggaccgcac cgggcggtcg
     2641 gtcagcaggc tgaccagggc ggtggtcatc gtcacgcccg cgctcgggcc gtccttgggc
     2701 accgcgccgg ccgggacgtg cacgtggtag cggcgcttct cgacgccagg gccgatgccc
     2761 agctcgcggg cgtgggaccg gacgtaggac agcgcgatcg cggccgactc cttcatcacg
     2821 tcgccgagct ggccggtgag ggtcaggccg ggctcgccct ccatcgcgct cgcctcgacg
     2881 aagagcacgt cgccaccggt gccggtgacc gcgaggccgg tggccacgcc gggcaccgag
     2941 gtgcgctcgg ccgcctcgaa gaagaagcgc tgccggccga ggtactcgcc gacgtcgccc
     3001 gcgtccacga cgatcggggt gcccgcctcg ccggcggcca tcttcgtggc agccttgcgg
     3061 aggagcttgc cgatct
//
LOCUS       DASPYY010000600         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2629204, whole genome shotgun sequence.
ACCESSION   DASPYY010000600 DASPYY010000000
VERSION     DASPYY010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629204"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1103)
                     /locus_tag="VEP73_09495"
     CDS             complement(30..1103)
                     /locus_tag="VEP73_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HYT26700.1"
                     /translation="MNRDALKLTVYFGERDLVGGDGFLADALLDLYGRHGFQTSVLLR
                     GVEGFGLKYRLHTQRLLTLSEVLPLVAVAVDTRERIQQALPEVAALVGEGLITLERAR
                     LLVGRVRAVRFPEALTEETKLTIYLGRQERAGGAPAFLAVVDLLHRHGVSGATVLLGV
                     DGTMVGFRQRARFFGRNANVPLMVIAVGPGEVIARVLPELGALLERPLVTLERVRVCK
                     RDGVRLAEPRGLPDADPSGLGIWQKLMVYTEGAARHGDRSLYVELVRRLREAGAAGAT
                     VLRGMWGYAGDHAPHGDRLLALRRRVPAVTVIVDTPTRIRCWFEIVDELTDETGLVTS
                     ELIPAMRATGPGTVEGGLGLSSL"
     gene            complement(1100..1465)
                     /gene="crcB"
                     /locus_tag="VEP73_09500"
     CDS             complement(1100..1465)
                     /gene="crcB"
                     /locus_tag="VEP73_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HYT26701.1"
                     /translation="MSVLVWLGVGLLGGLGALGRFALDAVVSSRAGRRFPVGTLVVNL
                     TGALALGLLAGLPVTGRALLLAGTATVGSYTTFSTWMLETHRLGEDGELGPAAVNLVL
                     SLMAGAAAAALGRAVGAAL"
     gene            complement(1462..>1660)
                     /locus_tag="VEP73_09505"
     CDS             complement(1462..>1660)
                     /locus_tag="VEP73_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HYT26702.1"
                     /translation="YRRPLLGTGLCGALTTFSTMQLELLRMLDGGRVALAVGYGAASV
                     AAGFVAVAAATALVRRVRALR"
BASE COUNT          252 a          628 c          607 g          173 t
ORIGIN      
        1 ggccggccac ccagcggtcc aacgccgtat cacaagcttg acaggcccag gccgccctcg
       61 accgtcccgg gaccggtcgc gcgcatggct gggatcagct cgctggtgac gagaccggtc
      121 tcgtcggtca gctcgtcgac gatctcgaac cagcagcgga tccgcgtcgg ggtgtcgacg
      181 atgacggtga ccgccgggac gcggcggcgc agggcgagca gccggtcgcc gtggggggcg
      241 tggtcgccgg cgtagcccca catgccacgc agcacggtgg caccggccgc gcccgcctcg
      301 cgcaggcgcc ggaccagctc gacgtacagc gagcggtcgc cgtgccgggc ggctccctcg
      361 gtgtagacca tgagcttctg ccagatccca agtccgctcg ggtcggcgtc gggcaggccg
      421 cgcggctcgg ccagccgcac tccatcgcgc ttgcacaccc gcacccgctc gagcgtcacc
      481 agcgggcgtt ccagcagcgc gccgagctcg ggcaggaccc gggcgatcac ctcgccgggg
      541 ccgaccgcga tcaccatgag cggcacgttg gcgttgcggc cgaagaagcg tgcccgttgc
      601 cggaagccca ccatggtccc gtcgacaccg agcaggacgg tggcgcccga gacgccatgc
      661 cggtggagca ggtcgacgac ggcgaggaac gccggcgccc cgcctgcccg ctcctgacgc
      721 ccgaggtaga tggtgagctt ggtctcttcg gtgagggcct cggggaaccg cacggcgcgg
      781 acccgcccga ccagcagccg ggcccgctcc agggtgataa gcccctcgcc gacgagggcg
      841 gcgacctctg gcagcgcctg ctggatgcgc tcgcgggtgt cgaccgcgac cgccacgagc
      901 ggcaggacct ccgacagggt cagcagccgc tgggtgtgca gccggtactt gaggccgaac
      961 ccctcgacgc cgcgcagcag cacgctggtc tggaagccgt gccgcccgta caggtcgagc
     1021 agcgcgtcgg cgaggaagcc gtcaccgccg accaggtcgc gctcgccgaa gtagacggtg
     1081 agcttcaggg cgtcgcggtt cacagcgccg ctccgaccgc ccgcccgagc gcggcggcgg
     1141 cggcgcccgc catcaggctg agcacgaggt tgaccgcggc cgggccgagc tcgccgtcct
     1201 cgccgagccg gtgggtctcc aacatccagg tcgagaaggt ggtgtaggac ccgacggtgg
     1261 ccgtgcccgc cagcagcagc gcacggccgg tgaccggcag gccggcgagc agcccgagcg
     1321 caagcgcgcc ggtgaggttg accaccaggg tgccgaccgg gaaccgccgg ccggcgcggc
     1381 tggacacgac cgcgtcgagc gcgaagcggc caagcgcacc caggccgccg agcagcccca
     1441 cgccaagcca gacgagcacg ctcatcgcag cgcccggacc cgccggacca gcgcggtcgc
     1501 ggccgccacg gccacgaagc cggcggccac gctcgcggcc ccatagccga ccgccaaggc
     1561 gacgcgcccg ccgtcgagca tccgcagcag ctccagctgc atcgtggaga acgtggtcag
     1621 cgcgccgcac aggcccgtgc cgagcagcgg ccgccggtag
//
LOCUS       DASPYY010000601         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1507217, whole genome shotgun sequence.
ACCESSION   DASPYY010000601 DASPYY010000000
VERSION     DASPYY010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507217"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1052)
                     /locus_tag="VEP73_09510"
     CDS             complement(165..1052)
                     /locus_tag="VEP73_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT26703.1"
                     /translation="MAERGTVTARAGAPSRPARGRRPSPGPLLLRQIRHQNLLFWRNP
                     FSAFFTMLFPLVFLLLFNTLQGSERLASRGGIRFAQFLTPGILAFAVVSACYTNLATS
                     VPINRDEGILKRVRGTPLPPWVYVAARIGSAVWMALIASVLMIAVGVALFHFRVYWHL
                     LPAAAVTLVLGAACFCACGLAVASVIPNGEAAPAVANFTVLPVIFVSDIFYPLDKAPT
                     WLKVAGSLFPVKHFALAIEQDFSPFTAGLGFRWDHLAVVAAWLVVATLVAVRKFRWEP
                     SADTSGGGRTRGRGRRAAG"
     gene            complement(1045..>1763)
                     /locus_tag="VEP73_09515"
     CDS             complement(1045..>1763)
                     /locus_tag="VEP73_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT26704.1"
                     /translation="QSSGIHRDLTVEEVVSLFGGYYPHPRPTGEVIELVGLEEKRSVR
                     TKALSGGQRRRLDLALGIVGDPDLIFLDEPTTGFDPSARRRSWELVDSLRSLGKTILL
                     TTHYMDEAQNLADRVAVLAGGRIVAAGSPESLGGRDVAEAVVTFRLPAGTGAADLPPG
                     LPETLRVEGSHVAVRTVAPTRTLNLLTGWALARGEELEALAVTRPSLEDVYLQLTGVD
                     EGKPGEARPDEALAGEARHG"
BASE COUNT          262 a          629 c          680 g          192 t
ORIGIN      
        1 ggacgccaag ctcgccggga tccgcgcgca cgccagccag gtgggcgacc cggccgagct
       61 cgagcgccgg gtgcgcacct ggaacgccgc gatcggggcc gagttcgggc tcggcgcggc
      121 cgaggcgttc cacaccctgg acaccagggg gcgttgacgg ccggtcagcc ggctgcccgg
      181 cgcccgcggc cccgcgtgcg gccgccgccc gaggtgtccg cgctcggctc ccagcggaac
      241 ttccgcaccg cgacgagcgt cgccacgacg agccacgcgg cgaccacggc gaggtggtcc
      301 caccggaagc ccaggccggc ggtgaagggg ctgaagtcct gctcgatggc gagcgcgaag
      361 tgcttgaccg ggaagaggga gccggcgacc ttcagccagg tgggcgcctt gtccagggga
      421 tagaagatgt cggagacgaa gatcacgggc agcacggtga agttggcgac cgcgggggcc
      481 gcctcgccgt tggggatcac cgaggccacc gccagcccgc aggcgcagaa gcaggccgcg
      541 ccgagcacca gggtgacggc cgcggccggc aggaggtgcc agtacaccct gaagtggaag
      601 agcgccacgc cgacggcgat catgagcacg gaggcgatca gggccatcca cacggccgac
      661 ccgatccggg cggcgacgta gacccagggg ggcagcgggg tgccccgcac ccgcttgagg
      721 atgccctcgt cccggttgat cggcaccgac gtggccaggt tcgtgtagca cgccgacacc
      781 accgcgaagg ccaggatgcc cggggtgagg aactgcgcga agcggatgcc gccgcggctg
      841 gcgagccgct cgctgccctg cagcgtgttg aacagcagca ggaacacgag cgggaacagc
      901 atcgtgaaga aggccgagaa cgggttgcgc cagaagagca ggttctggtg gcggatctgg
      961 cggaggagca gcgggccggg cgacggccgg cggcccctgg ccggccgtga cggcgccccg
     1021 gcgcgcgccg tcacggtgcc gcgctcagcc atgccgggcc tcccccgcca gggcctcgtc
     1081 cggccgggcc tcccccggct tgccctcgtc gacgccggtg agctgcaggt agacgtcctc
     1141 gagagagggc cgcgtgaccg cgagggcctc cagctcctcg ccgcgggcga gggcccagcc
     1201 ggtgagcagg ttcagggtgc gggtcggcgc cacggtccgg accgcgacgt ggctgccctc
     1261 gacccggagc gtctcgggca gcccgggcgg caggtcggcc gctcccgtgc cggccggcag
     1321 ccggaaggtg acgaccgcct cggccacgtc gcggccgccg agagactccg ggctgcccgc
     1381 ggccacgatc cggccgccgg ccagcacggc gacccggtcg gccaggttct gggcctcgtc
     1441 catgtagtgg gtcgtcagca ggatcgtctt gccgagcgag cgcaggctgt cgaccagctc
     1501 ccaggagcgg cgccgcgcgg acgggtcgaa gccggtggtc ggctcgtcga ggaagatgag
     1561 gtccgggtcc ccgacgatcc ccagggcgag gtcgaggcgg cgccgctggc cgcccgacag
     1621 cgccttggtc cgcaccgagc gcttctcctc gagccccacc agctcgatca cctcgccggt
     1681 gggccgcggg tgcgggtagt acccgccgaa gagcgagacg acctcctcca ccgtcaggtc
     1741 ccggtggatg cccgacgact gca
//
LOCUS       DASPYY010000602         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1084791, whole genome shotgun sequence.
ACCESSION   DASPYY010000602 DASPYY010000000
VERSION     DASPYY010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084791"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1306)
                     /gene="mftD"
                     /locus_tag="VEP73_09520"
     CDS             complement(74..1306)
                     /gene="mftD"
                     /locus_tag="VEP73_09520"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499235.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HYT26705.1"
                     /translation="MKLANPWKQNPWFESVAVAQRRARKLLPAPVYASLLAGSERGQT
                     VSDNQAAFAELGLAPHVAGHQPKRAQATTLLGQELSLPVVISPTGVQAVHPGGEVAVA
                     RAAAARGTLMGLSNFASKPVEEVTATGAPTLFQMYWTGDRDVMAQRMRRAHAAGVTGL
                     IATLDWSFSVGRDWGSPEIPEKVDLKTAVRMAPQVITKPRWLYQFGRAGHLPDLTVPN
                     LAPPGGAAPTFFGAYYEWMTTQPPSWDDVAWMAQEWHQLSGTPFLLKGVCRVDDARRA
                     VDAGVDAISVSNHGGNNLDGTPATIRLLHPIAEAVGDQVEVVMDGGIRRGSDVVKALA
                     LGARAVLIGRAYLWGLAANGQAGVENVLDILRGGIDSALLGLGLSSIHELTPDHLLIP
                     EGFHRVAGAGHGPRRASP"
     gene            complement(1303..>1524)
                     /locus_tag="VEP73_09525"
     CDS             complement(1303..>1524)
                     /locus_tag="VEP73_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HYT26706.1"
                     /translation="RGYGEAALAAPRTIPGASQDHSRTTQARNQPVLLQLRTRRPAGP
                     DRPAAGLAPPVSACAQSPLAGFVPEGSAR"
BASE COUNT          197 a          531 c          593 g          203 t
ORIGIN      
        1 aggcggcgca cggtggcggc gccgatgccc cgtgccgccc cggtgacgag ggcgacccgg
       61 ggctgatcgc tggtcacggc gatgctcgcc gcggcccgtg gccggcgccg gcgacgcggt
      121 ggaagccttc gggaatgagc aggtgatcgg gcgtgagctc gtggatcgag gagagcccta
      181 gcccgagcag cgccgagtcg atgccgccgc gcaggatgtc gaggacgttc tcgacgccgg
      241 cctggccgtt cgcggccagt ccccacaggt aggcgcgccc gatgaggacc gcgcgggcgc
      301 cgagggcgag cgccttgacg acgtcggagc cccggcggat gccgccgtcc atgacgacct
      361 caacctggtc gccgaccgcc tcggcgatcg gatgcaggag cctgatggtg gcgggagtgc
      421 cgtcgaggtt gttgccgccg tggttggaga ccgagatcgc gtcgaccccg gcgtcgacag
      481 cgcggcgggc gtcgtcgacc cggcacacgc ccttgagcag gaacggcgtg ccgctgagct
      541 ggtgccactc ctgcgccatc caggcgacgt catcccagga tggcggctgc gtggtcatcc
      601 actcgtagta agcgccgaag aacgtcggcg cagcgcctcc gggcggggcg aggttcggga
      661 cggtcaggtc gggcagatgg ccggcccggc cgaactggta gagccagcgc ggcttggtga
      721 tgacctgcgg cgccatccgc accgcggtct tcaggtcgac cttctccggg atctccgggg
      781 agccccagtc ccgcccgacc gagaacgacc agtcgagggt ggcgatcaga ccggtcacgc
      841 cggccgcgtg cgcgcgccgc atgcgctggg ccatcacgtc cctgtccccc gtccagtaca
      901 tctggaacag cgtgggcgcc ccggtggcgg tgacctcctc gacgggcttg gacgcgaagt
      961 tcgacagtcc catcagcgtg ccgcgcgccg cggccgcgcg ggcgacggct acctcgccgc
     1021 cggggtgcac agcctggacc ccggtcggcg agatgaccac gggcagcgag agttcctgcc
     1081 cgagcagcgt ggtggcctgt gctctcttcg gctggtggcc agcgacgtgc ggcgcgaggc
     1141 ccagctcggc gaacgcggcc tggttgtcgg agactgtctg gccgcgctcg gacccggcga
     1201 gcagggacgc gtagacgggc gccggcagca gcttgcgggc gcgccgctgc gcgaccgcga
     1261 ccgactcgaa ccaggggttc tgcttccagg ggttcgccag cttcaccgcg cgctcccctc
     1321 cggcacgaac ccggccagcg ggctctgcgc gcacgcgctc accggtggtg cgaggccagc
     1381 agccggccgg tcgggcccgg ccgggcggcg ggtgcgcagc tgcagcagca cgggctgatt
     1441 gcgggcctgg gtggtcctgg agtggtcctg gctcgcaccg gggatggtgc gcggggcggc
     1501 gagcgcggcc tcgccgtagc cacg
//
LOCUS       DASPYY010000603         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1929077, whole genome shotgun sequence.
ACCESSION   DASPYY010000603 DASPYY010000000
VERSION     DASPYY010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929077"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VEP73_09530"
     CDS             <1..538
                     /locus_tag="VEP73_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HYT26707.1"
                     /translation="LEGRRPLVTEVQALVASTSAPMPRRTAQGLDPGRLAILVAVLER
                     RSRIALSGQDVFAATVGGVRLTEPAGDLAVCLALASAARDWEVPSELVAFGEVGLGGE
                     VRPVPQLPRRLAEAARLGFRRALVPAGADARADALAVVEVDSVTHALSILLASGRGTA
                     RPGRPPAGAASPSPGSHR"
     gene            535..1611
                     /gene="disA"
                     /locus_tag="VEP73_09535"
     CDS             535..1611
                     /gene="disA"
                     /locus_tag="VEP73_09535"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HYT26708.1"
                     /translation="MTCSQFDDRLIQVLQQIAPGTHLREGLERVLRAEKGALVVLGAP
                     PQVQALFTGGFRIDVRFTAQRLSELAKMDGALVLDEAARNILWANVHLMPDPAVPTSE
                     TGTRHRTAERVARQTGVPVVTVSESMHILTLYLDELRHVFENVANVLSRGNQAAQTLE
                     HYRARFDEVAGHLSNLELEGTATQRDVLAVLARAELLHRIAAELERYVAELGVDGRLL
                     RLQLDELSDGVDEAKVLVVRDHLAARPGYGVPDVLAELAALTPEELSDTITLAKVVNL
                     VEDPEALDAVLTARGYRILAKVPELSPSLIDRVAQRFGSLKVLLAASLEDLEEVEGVG
                     RERARQVREGLGRLAEGSAAEYYP"
     gene            1740..>2127
                     /locus_tag="VEP73_09540"
     CDS             1740..>2127
                     /locus_tag="VEP73_09540"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYT26709.1"
                     /translation="MFKVGDSVIYPQHGAATIQGLQDREVGGETREYLVLRLTYGDLT
                     LMVPRDNCQEVGIREVCTSDDVEGVFEILRNGESSTQGNWSRRFKGNIERLQSGDIFQ
                     VAEVVRDLTVRDRDKGLSAGEKRMLQR"
BASE COUNT          287 a          770 c          763 g          307 t
ORIGIN      
        1 cctcgagggc cgccgcccgc tcgtcaccga ggtgcaggcg ctggtggcgt cgacctcagc
       61 gcccatgccg aggcggaccg cacagggcct cgaccccggc cggctcgcca tcctggtcgc
      121 cgtgctggag cggcggtccc gcatcgccct cagcggccag gacgtcttcg cggcgaccgt
      181 cggcggcgtg cgcctcaccg agccggccgg cgacctcgcc gtctgcctgg ccctcgcgtc
      241 ggccgcccgg gactgggagg tcccctccga gctggtcgcg ttcggggagg tggggctcgg
      301 cggcgaggtg cgcccggtgc cccagcttcc ccgccggctg gccgaggcgg cgcgcctcgg
      361 cttccggcgc gcgctcgtcc cggccggtgc cgacgcccgc gccgacgccc ttgcggtcgt
      421 ggaggtcgac tcggtgaccc acgccctctc gatcctgctc gcgtccggcc gcgggacggc
      481 ccggcccgga cggccgcccg ccggggctgc ctcgccgtcc cccgggagcc atcgatgacc
      541 tgctcgcagt tcgacgaccg gctgatccag gtgctccagc agatcgcgcc gggcacccat
      601 ctccgcgagg ggctcgagcg ggtcctgcgc gccgagaagg gcgcgctcgt ggtgctcggc
      661 gccccgcccc aggtgcaggc cctgttcacg ggcgggttcc gcatcgacgt gcggttcacc
      721 gcccagcgcc tctcggagct ggccaagatg gacggcgccc tcgtcctcga cgaggccgcc
      781 aggaacatcc tgtgggcgaa cgtgcacctg atgcccgacc cggcggtccc cacctcggag
      841 acgggcacgc ggcaccggac cgctgagcgg gtggccaggc agaccggcgt gccggtggtg
      901 acggtctccg agtccatgca catcctcacc ctgtacctgg acgagctgcg ccacgtgttc
      961 gagaacgtgg ccaacgtgct ctcgcggggc aaccaggcgg cgcagaccct cgagcactac
     1021 cgggcccggt tcgacgaggt cgcgggccac ctctcgaacc tcgagctcga gggcaccgcc
     1081 acccagcgcg acgtgctcgc ggtcctcgcc agggccgagc tgctgcatcg catcgccgcc
     1141 gagctcgagc gctacgtcgc cgagctcggc gtcgacgggc gcctgcttcg cctgcagctc
     1201 gacgagctgt cggacggcgt cgacgaggcc aaggtcctcg tggtccgcga ccacctcgcg
     1261 gccaggcccg ggtacggcgt gcccgacgtc ctcgccgagc tggccgcgct gacgccggag
     1321 gagctgtccg acaccatcac gctggcaaag gtcgtgaacc tcgtcgagga cccggaggcg
     1381 ctggacgcgg tgctgacggc ccgtggctac cggatcctcg cgaaggtgcc tgagctgtcg
     1441 ccgtcgctga tcgaccgggt ggcccagcgc ttcggctcgc tcaaggtcct gctggccgcc
     1501 agcctcgagg acctggagga ggtcgagggg gtcggccgcg agcgggccag gcaggtccgg
     1561 gaagggctcg ggcgtctcgc cgaggggtcg gcagccgagt actacccgtg agcgcgctgc
     1621 gcggccgacc ctgtccgtca agtcccgatc aggccctcta cctgcggatt catttgaaac
     1681 cggcgagacg aaagtgctaa gatggcatgc ttcatctcga cgggaagggg tattcgcgta
     1741 tgttcaaggt cggggacagc gtcatctacc cgcagcacgg ggctgcaacg atccaggggt
     1801 tgcaggaccg ggaggtcggt ggcgagaccc gagagtatct cgtgctcagg ctgacgtacg
     1861 gcgacctcac cctgatggtg ccgcgcgaca actgccagga ggtcgggatc cgcgaggtct
     1921 gcacctccga cgatgtcgag ggcgtgttcg agatccttcg caacggcgag tcctcgacgc
     1981 agggcaactg gagtcgccgg ttcaagggca acatcgagcg gctccagtcg ggcgatatct
     2041 tccaggtggc cgaggtcgtt cgcgatctca ccgtccggga ccgcgacaag ggcctctccg
     2101 ccggagagaa gcgcatgctc cagcgtt
//
LOCUS       DASPYY010000604         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_382052, whole genome shotgun sequence.
ACCESSION   DASPYY010000604 DASPYY010000000
VERSION     DASPYY010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382052"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1583
                     /locus_tag="VEP73_09545"
     CDS             <1..>1583
                     /locus_tag="VEP73_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HYT26710.1"
                     /translation="PALGTQTSDHARLLEALEREWGLTGLTADIHVVQGLQQALRKGE
                     WAVTVAVHDGSAITAVWPGFRDVALGIAFDVGSTTVAGHLCDLATGEVLASAGAMNPQ
                     IRFGEDLMSRVSYVMLNPGSTAELTHAVRGCLNDLVVALVAEAGREPGDVLEVTLVGN
                     PVMHHLALGIDPTELGGAPFALATGQALRLRAATELELGVHPGARAYVLPCIAGHVGA
                     DAAGVLLAEAPHQRDDLSLLVDVGTNAEIVLGNRDRLLAASSPTGPAFEGAQISAGQR
                     AAPGAIERVRIDRDTLEPRLRVIGNPAWSDQPGFDPAAVSGICGSGIIEAVAELYLAG
                     VVTAAGVIDGAAAARSSRVVPDGRTYAYVLHDRALQRILVTQNDIRQIQLAKAALHAG
                     CRLLMDRLGVDQVDRVRLAGAFGAHIDPVHALVLGMVPDCDPAAVSSAGNAAGTGARI
                     ALLSGRSRREVEEAVRRVEKVETAVEPRFQEHFVDAMAIPHRRDPYARLAKVVTLPER
                     DLDANGRMHPRTRTRRRTR"
BASE COUNT          205 a          630 c          544 g          204 t
ORIGIN      
        1 cccgccctcg gcacccagac cagcgaccac gcccgcctcc tcgaagcgct ggagcgcgag
       61 tggggcctca ctggtctgac cgccgacata cacgtcgtcc agggcctcca gcaggcgctg
      121 cggaaggggg agtgggccgt caccgtcgcc gtccacgacg gcagcgcgat cacggcggtc
      181 tggccgggct tccgcgacgt cgccctcggg atcgccttcg acgtcggctc gaccaccgtc
      241 gccgggcacc tctgcgacct cgccaccggc gaggtgctcg ccagcgcggg cgcgatgaac
      301 ccacagatcc gcttcggcga ggacctgatg agccgcgtca gctacgtgat gctcaacccg
      361 ggctcgaccg ctgagctgac ccacgcggtc cgcggctgcc tcaacgacct ggtcgtcgcc
      421 ctggtcgccg aggcgggccg cgagcccggc gacgtgctgg aggtgaccct ggtcggcaac
      481 ccggtgatgc accacctggc gctcggcatc gacccgacgg agctgggcgg tgcgccgttc
      541 gcgctggcca ccggccaggc gctgcgcctg cgcgccgcca ccgagctgga gctgggcgtg
      601 catccgggcg cccgcgccta cgtcctgccg tgtatcgccg ggcacgtcgg cgccgacgcc
      661 gccggggtgc tgctggccga ggcgccccac cagcgcgacg acctgtcgct gctggtggac
      721 gtcggcacca acgccgagat cgtgctcggc aaccgcgacc ggctgctggc cgcctccagc
      781 cccactggcc ccgcgttcga gggcgcgcag atctccgccg ggcagcgcgc cgcacccggc
      841 gcgatcgagc gcgtccgcat cgaccgcgac accctggagc cgcgcctccg cgtgatcggc
      901 aacccggcct ggtccgacca gccgggcttc gatcctgctg ctgtcagcgg catctgcggc
      961 tccgggatca tcgaggcggt cgccgagctg tacctcgccg gggtcgtcac cgccgccggc
     1021 gtgatcgacg gcgccgccgc cgcgcgctcc tcccgggtcg tccccgacgg gcgcacgtat
     1081 gcctacgtgc tgcacgaccg cgccctccag cggatcctgg tcacccagaa cgacatccgc
     1141 cagatccagc tcgccaaggc cgccctgcac gccggctgcc ggctgctgat ggaccgcctc
     1201 ggtgtggacc aggtcgaccg ggtccgcctg gcgggcgcgt ttggcgccca tatcgacccg
     1261 gtccacgccc tcgtgctcgg catggtcccc gactgcgacc ccgcggcggt cagcagcgcc
     1321 ggcaacgccg ccgggaccgg cgcccgcatc gcgctgctct ctggccgctc ccgccgcgag
     1381 gtcgaggaag ccgtgcggcg cgtcgagaag gtcgagaccg ccgtcgagcc gcgcttccag
     1441 gaacacttcg tcgacgcgat ggcgattccg caccgccgtg acccctacgc ccgcctcgcc
     1501 aaggtggtca cgctccccga gcgcgatctg gacgcgaacg ggcggatgca ccccaggacc
     1561 aggaccagaa ggaggacccg gtg
//
LOCUS       DASPYY010000605         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1788040, whole genome shotgun sequence.
ACCESSION   DASPYY010000605 DASPYY010000000
VERSION     DASPYY010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788040"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VEP73_09550"
     CDS             <1..274
                     /locus_tag="VEP73_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26711.1"
                     /translation="APGRQLAAAALPGVEPAVLRDRGAAAARPNDRRDPRGRPGPAPA
                     GVLAAVVLAGAMLLPPAAVRPAPRRPRRPGRHAPAAPRAPPLLQPA"
     gene            323..>1531
                     /gene="secD"
                     /locus_tag="VEP73_09555"
     CDS             323..>1531
                     /gene="secD"
                     /locus_tag="VEP73_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYT26712.1"
                     /translation="MSRHLGWRLLALLVLLGAAGALVDTRPPRLGLDLRGGTQIVLEA
                     RAAGRQKAGADTLDRTLEVLRRRVDQLGVAEPTLQRSGDRRVIVELPGVYDPRQAVDV
                     IGRTAQLTFHPVLGVAEAGTGTGTPATPGRDGVLVLADEDGTRLRLGPARLTGRAVGD
                     ARAALDAQTSTAWQVQVDFRDAGGRRWAELTGEAACEPAGDPRRRVAIVLDQRVVSSP
                     QVDPSVGCGQGIAGGQTVITGGFSDSDAKDLALLIRAGALPVPVQVVEQRTIGPTLGA
                     AAIDASVKAAAIGAALTILFMIAYYRLLGGLAAVALASYGLLSFAVLLALGSTLTLPG
                     IAGFVLAIGMAVDANVLVFERMKEEHRAGASLRVATERGFGRAFSAIADSNATTLLAA
                     GLLFFLASGAV"
BASE COUNT          151 a          627 c          569 g          184 t
ORIGIN      
        1 cgcgcccgga cgccagctcg ccgcggccgc gctgcccggc gtcgagccgg cggtgctgcg
       61 cgaccggggc gccgcggccg cccgtcccaa cgaccggcgg gacccacgcg gccggcccgg
      121 gccggcgccg gccggcgtcc tcgccgcggt cgtgctggcc ggtgccatgc tgctcccgcc
      181 ggcggccgtc cggccggcgc cccggcgccc gcgccggccc ggccggcacg cccccgcggc
      241 cccgcgggcg ccgccgctcc tccagcccgc ctgaccacgc ccgccgtgcc cccaggcgcg
      301 gtccacgctg gaggtccacc tcatgtcacg ccatctcgga tggcggctcc tcgccctcct
      361 cgtcctcctc ggcgccgccg gtgccctggt ggacacccgc ccgccccgcc tcggcctcga
      421 cctgcgcggc ggcacccaga tcgtcctgga ggcgcgcgcc gccggccgcc agaaggccgg
      481 tgccgacacc ctggaccgca ccctggaggt cctgcgccgg cgggtcgacc agctcggcgt
      541 ggccgagccg accctgcagc gctcggggga ccgccgcgtc atcgtggagc tgcccggcgt
      601 ctacgacccc aggcaggcgg ttgacgtcat cggcaggacg gcccagctca ccttccatcc
      661 ggtgctcggc gtggccgagg ccgggaccgg gacggggacg ccggccacgc cagggcggga
      721 cggcgtgctg gtcctcgccg acgaggacgg cacccgcctg cggctcggcc cggcccggct
      781 caccggccgc gcggtcggcg acgcccgcgc cgccctcgac gcccagacca gcaccgcctg
      841 gcaggtgcag gtcgacttcc gcgacgcggg cgggcggcgg tgggccgagc tgaccggcga
      901 ggcggcgtgc gagccggccg gggacccaag gcggcgggtc gccatcgtgc tcgaccagcg
      961 ggtcgtctcg agcccgcagg tcgacccgtc cgtcggctgc gggcagggga tcgcgggcgg
     1021 ccagacggtg atcaccggcg gcttctccga ctccgatgcc aaggacctgg cgctgctcat
     1081 ccgtgccggc gccctgcccg tcccggtgca ggtggtcgag cagcgcacca tcggcccgac
     1141 cctgggcgcg gccgcgatcg acgcgagcgt caaggcggcc gcgatcggcg ccgcgctgac
     1201 gatcctgttc atgatcgcct actaccggct gctcggcggg ctcgccgcgg tggccctggc
     1261 aagctacggc ctgctgtcct tcgcggtgct gctcgcgctg ggctccaccc tcaccctgcc
     1321 cggcatcgcc gggttcgtgc tcgcgatcgg catggcggtc gacgccaacg tgctggtgtt
     1381 cgagcgcatg aaggaggagc accgggccgg cgcgagcctg cgggtcgcca ccgaacgggg
     1441 cttcggcagg gcgttcagcg cgatcgccga ctcgaacgcg accacgctgc tcgccgccgg
     1501 gctgctgttc ttcctcgcgt ccggggcggt t
//
LOCUS       DASPYY010000606         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2490984, whole genome shotgun sequence.
ACCESSION   DASPYY010000606 DASPYY010000000
VERSION     DASPYY010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490984"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VEP73_09560"
     CDS             complement(<1..825)
                     /locus_tag="VEP73_09560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HYT26713.1"
                     /translation="MAPPIRRIGILTSGGDAPGLNAVIRAVVKTAEVEYGWTVVGIEH
                     GFEGLIGKPKVRPLDAVAVSGLLPRGGTILGSTNKGHFALMDLGGELQRDPAPYKELA
                     GNARRLGIDALVVIGGEGSQGIASELARYGVRAMGVPKTIDNDLWGCEQTFGFDTALT
                     VATEAIDRLHTTAASHDRVMVLEVMGRDTGWIALHSGIGGGADIILIPEIPFTVEGIV
                     NKVTTREFQGSSFSIVVCSEGAEELGSGQMYQEATGTLGGVGAHVATRVAKETGKDA"
     gene            complement(779..>2051)
                     /gene="lnt"
                     /locus_tag="VEP73_09565"
     CDS             complement(779..>2051)
                     /gene="lnt"
                     /locus_tag="VEP73_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYT26714.1"
                     /translation="LGASQWNDRPVLALASVGGVWAVSFLVAAVNVAVAVALAPRVAA
                     AGPWRPGRRRAARLAAVAALAALTAGTLGYGWLRPAPAVTRTVRAAVVQPGIIHQTEP
                     RFRASETATLALAPARPDLVVWGESSVGRDPATHPGDVARLVAAARAVGAPILANVDA
                     QRSSRVPGEGATAGPGIFKTSLLVGPDGPEGSYDKMRLVTFGEYIPLRPLLGWVGRFT
                     AAATEDRKRGTHLAVLDAGPVTVGPLICFESAFPDLGRSLAARGADLVVVQSATTTFQ
                     GTWGPDQHASLAAVRAVESGRPVVQAAISGVSAAFDARGRRLAWYPGTWRGAAVVAVP
                     LSRERTPYDRWGDRLPAACWVVLALAALVAAVRSRRDPHLGGPPRLGGRQSSTVPRRG
                     VTLVASTHPHKGTRTANGTSDPPNRHPHQRG"
BASE COUNT          254 a          762 c          789 g          246 t
ORIGIN      
        1 ggcgtccttg ccggtctcct tggcgacccg cgtcgcgacg tgggcgccga ccccgccgag
       61 cgtgcccgtg gcctcctggt acatctggcc gctgcccagc tcctcggcac cctccgagca
      121 gacgacgatc gagaagctcg acccctggaa ctcgcgcgtc gtcaccttgt tcacgatccc
      181 ctcgaccgtg aacgggatct ccgggatgag gatgatgtcg gcgccgccgc cgatgccgga
      241 gtgcagcgcg atccagcccg tgtcccggcc catgacctcg aggaccatga cccggtcgtg
      301 gctggcggcg gtcgtgtgga gccggtcgat cgcctcggtg gccaccgtga gcgcggtgtc
      361 gaacccgaac gtctgctcgc agccccagag gtcgttgtcg atcgtcttcg ggacccccat
      421 ggcccgcacc ccgtacctgg cgagctcgct ggcgatgccc tggctgccct cgccgccgat
      481 cacgaccagg gcgtcgatgc cgagccggcg ggcgttaccg gccagctcct tgtagggggc
      541 ggggtcgcgc tggagctccc cgccgaggtc catgagcgcg aagtggccct tgttggtcga
      601 gcccaggatc gtcccccctc gggggagcag gccgctgacc gccacggcgt cgagcggccg
      661 gaccttgggc ttgccgatca ggccctcgaa gccgtgctcg atgccgacga ccgtccagcc
      721 gtactcgacc tcggcggtct tgaccacggc gcggatcacg gcgttcaggc cgggggcgtc
      781 acccccgctg gtgaggatgc cgattcggcg gatcggaggt gccattggcc gtccttgtgc
      841 ccttgtgcgg gtgggtcgac gctacgagag taacgcctcg ccggggcacg gtcgaggact
      901 gtcgcccgcc gagccgcggc ggcccgccca ggtgcgggtc gcggcggctg cgcacggccg
      961 cgacgagggc ggcgagcgcg agcacgaccc agcacgcggc cgggagccgg tcgccccagc
     1021 ggtcgtacgg cgtccgctcg cgggagagcg ggaccgcgac cacggcggcg ccgcgccagg
     1081 tgcccgggta ccaggcgagg cggcggccgc gggcgtcgaa ggcggccgac acgccgctga
     1141 tggcggcctg cacgaccggg cggccggact cgaccgcacg caccgcggcc aggctcgcgt
     1201 gctggtcagg accccaggtg ccctggaagg tggtggtcgc gctctgcacc acgaccaggt
     1261 cggcgccgcg ggcggcgagc gaccggccca ggtcggggaa cgccgactcg aagcagatga
     1321 gcgggccgac cgtcaccggc ccggcgtcga ggacggcgag gtgggtgccg cgcttgcggt
     1381 cctccgtcgc ggcggcggtg aagcgtccca cccagccaag gaggggccgc agcgggatgt
     1441 actcgccgaa cgtcaccagg cgcatcttgt cgtagctgcc ctcggggccg tccggcccga
     1501 cgagcagcga ggtcttgaag atgccgggac cggcggtggc cccctcgccc ggcacgcggg
     1561 aggaccgctg ggcgtccacg ttcgcgagga tgggcgcgcc gacggcgcgc gcggcggcga
     1621 cgagccgggc gacgtccccc gggtgggtgg ccgggtcgcg cccgacgctg ctctcgcccc
     1681 agacgaccag gtcgggccgg gcgggcgcca gcgcgagggt ggcggtctcg ctcgcgcgga
     1741 agcgaggctc cgtctggtgg atgatgcccg gctgcacgac cgccgcccgc acggtgcggg
     1801 tcacggcggg cgcgggccgc agccacccgt acccgagcgt cccggccgtg agcgcggcca
     1861 gggcggcgac cgcggccagc cgcgccgccc ggcgccgccc ggggcgccac ggcccggccg
     1921 cggccaccct gggggcgagc gccacggcaa cggcgacgtt cacggcggcg accaggaagc
     1981 tcaccgccca cacgccgccg accgaggcca gcgccagcac cgggcggtcg ttccactggc
     2041 tggcgccgag c
//
LOCUS       DASPYY010000607         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1788497, whole genome shotgun sequence.
ACCESSION   DASPYY010000607 DASPYY010000000
VERSION     DASPYY010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788497"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1349)
                     /locus_tag="VEP73_09570"
     CDS             complement(9..1349)
                     /locus_tag="VEP73_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HYT26715.1"
                     /translation="MTVRDLIDPAAAATAGEDMWKLMAELYPVCRSITGAGFRATLDV
                     LARHVDLDVHEVPSGTRAFDWTVPREWNIADAYVENAAGERVIDFRRSNLHVMSYSVP
                     VDARMPLSELRAHLHTLPEQPDWIPYRTSYYADNWGFCLSQRQLDALPDGEYHVVVDS
                     VLGEGSLAWGECLVPGATTDGATTDGATTDEVLVSCHACHPSLCNDNLSGVVLATFLA
                     KALAAASPRLSYRFLFIPGTIWSIVWLSRNEATLDRIKAGLVLACVGDPGHLTYKRSR
                     RGDAEIDRAVASVLATRGAPYDVADFSPYGYDERQFCSPGFDLPVGCLNRTPHGQFPE
                     YHTSADDLDLVRPEHLADSYGACLEVMRVLEENATFVNQNPKCEPQLGKRGLYGAVGG
                     RSDTVQVQLAMLWVLNLSDGGHSLLDVAERSGLPFGVLADAAQALATAGLLVER"
     gene            complement(1346..1894)
                     /gene="rfbC"
                     /locus_tag="VEP73_09575"
     CDS             complement(1346..1894)
                     /gene="rfbC"
                     /locus_tag="VEP73_09575"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HYT26716.1"
                     /translation="MIFTETGLRGAFVVDLEPIADDRGFFARSFDQKEFAGHGLAPSF
                     VQCNVSQNRRRGTLRGMHWQAAPYEEGKLVRCVRGAIHDVIVDLRPESPTLGEHVAVT
                     LTAANRTMLYVPEGFAHGFLTLADDTEVFYQISAFHAPGHARGARWDDPQLGIDWPAE
                     VAVVSERDRAWPDLRLGRGGGA"
     gene            complement(1923..>2137)
                     /locus_tag="VEP73_09580"
     CDS             complement(1923..>2137)
                     /locus_tag="VEP73_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26717.1"
                     /translation="ATCERKVELLLEGFPSQRDRRWFTPDTFWALLRLRGVEAGSPSG
                     FAEGFHCHKAVLGPIMPGGPRAVAGP"
BASE COUNT          298 a          713 c          836 g          290 t
ORIGIN      
        1 gcccgcggtc agcgctcgac caggagcccg gcggtggcga gcgcctgggc cgcgtcggcg
       61 agcaccccga aggggaggcc cgagcgctcg gcgacgtcca gcaggctgtg cccgccgtcg
      121 gagaggttga gcacccagag catggcgagc tggacctgca cggtgtcgct gcggccgccg
      181 acggcgccgt agagcccgcg cttgccgagc tgcggctcgc acttcgggtt ctggttgacg
      241 aaggtggcgt tctcctcgag cacccgcatg acctccaggc aggcgccgta cgagtcggcc
      301 aggtgctcgg ggcggacgag gtcgaggtcg tcggctgacg tgtggtactc ggggaactgg
      361 ccgtgcgggg tccggttcag gcacccgacc ggcaggtcga agcctggcga gcagaactgc
      421 cgctcgtcgt agccgtacgg ggagaagtcg gcgacgtcgt agggcgcgcc gcgggtggcg
      481 aggacgctgg cgacggcccg gtcgatctcg gcgtcgccac gccggctgcg cttgtaggtg
      541 aggtggccgg ggtcgcccac gcaggcgagc acgaggcccg ccttgatccg gtccagcgtg
      601 gcctcgttgc gggagagcca cacgatcgac cagatcgtcc cggggatgaa caggaaccgg
      661 tagctgaggc gcggcgacgc ggcggccagc gccttggcca ggaaggtggc gagcacgacg
      721 ccggagaggt tgtcgttgca gagcgacggg tggcaggcgt ggcaggagac gagcacctca
      781 tcggtggtgg cgccgtcggt ggtggcgccg tcggtggtgg cgccgggcac caggcactcg
      841 ccccaggcga ggcttccctc acccaggacc gagtcgacca cgacgtggta ctccccgtcc
      901 gggagggcgt cgagctggcg ctgcgagagg cagaagcccc agttgtcggc gtagtaggag
      961 gtccggtacg ggatccagtc gggctgctct ggcagcgtgt ggaggtgcgc cctgagctcc
     1021 gacagcggca tgcgggcgtc gaccgggacg ctgtagctca tcacgtggag gttggagcgg
     1081 cggaagtcga tcacgcgctc cccggccgcg ttctcgacgt aggcgtcggc gatgttccat
     1141 tcccggggga cggtccagtc gaacgcccgg gtgccgctcg gcacctcgtg cacgtcgagg
     1201 tcgacgtggc gggccaggac gtcgagcgtc gcgcggaacc ccgctccggt gatgctccgg
     1261 cacaccgggt agagctcggc catgagcttc cacatgtcct cgccggcggt cgcggccgcg
     1321 gccgggtcga tcaggtcgcg gacggtcatg ccccacctcc ccgcccgagc cgcaggtcgg
     1381 gccaggcgcg gtcgcgctcg gacacgacgg cgacctcggc cggccagtcg atgccgagct
     1441 gggggtcgtc ccagcgggca ccgcgggcgt ggccgggcgc gtggaacgcc gagatctggt
     1501 agaagacctc ggtgtcgtcg gccagggtca ggaagccgtg cgcgaacccc tccggaacgt
     1561 agagcatggt gcggttcgcg gccgtcaggg tcacggcgac gtgctcgccg agggtgggcg
     1621 actccgggcg gaggtcgacg atcacgtcgt ggatggcccc gcggacgcag cggacgagct
     1681 tcccctcctc gtagggcgcc gcctgccagt gcatgccgcg cagggtgccc cgccggcggt
     1741 tctgggagac gttgcactgc acgaagctcg gcgcgaggcc gtgtccggcg aactccttct
     1801 ggtcgaagga ccgggcgaag aagccgcggt cgtcggcgat cggctcgagg tcgacgacga
     1861 aggcgcctcg caggccggtc tcggtgaaga tcatcgctgg cgctccccct ggtcggacgc
     1921 tgtcacgggc cggcgacggc ccgcggcccg cccggcatga tgggcccgag gaccgccttg
     1981 tggcaatgga acccctcggc gaacccggac ggcgagccgg cctcgacgcc ccgcagccgc
     2041 agcagggccc agaacgtgtc cggcgtgaac cagcgcctgt cgcgctggct cgggaacccc
     2101 tcgaggagca gctccacctt gcgctcgcac gtcgcgg
//
LOCUS       DASPYY010000608         1749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_382674, whole genome shotgun sequence.
ACCESSION   DASPYY010000608 DASPYY010000000
VERSION     DASPYY010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382674"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1749
                     /locus_tag="VEP73_09585"
     CDS             <1..>1749
                     /locus_tag="VEP73_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYT26718.1"
                     /translation="AALAAAYSGEGAATRVQVGGQLMFNHDVGRQVQRDLAKAEGISV
                     PITLLLLVVVFASAVAGALPLAVGGFAIVGTLLVLRVLAGVTDVSIYSLNLTTALGLS
                     LAIDYSLFIVSRYREELRPGRAPGDALVATMRTAGKTVLFSAATVAVSLTALLVFPLY
                     FLRSFCYAGIAVVALAATGAVVVLPALLALIGPRVNRLRLPIGRRRPAAAPGAPGEVG
                     GNGAWHRIAVTVMRRPLPVALTVIALLVLLGAPFLHVRFGLPDERVQPTSAPSRQVSD
                     AIRRGFASDDAAALSVVTTGAEGPAGHAADIDRYAGDLSALAGVARVDALTGTYTGGR
                     RVTGPGPASGRFAAPTATWLSVVPSVDANSPEGEAVVHRVRDLPAPFAVKVTGPSAQL
                     VDTKAALFGRLPAAAAIIAAVTFVVLFLFTGSVVIPAKALVLNLLSLTAVYGAMVWIF
                     QEGHLAGFLGFTATGTIDTSMPVLLFCIAFGLSMDYEVFLLSRIKEEHDRTGDTVTAV
                     AVGLERAGRIVTAAAALLTIVFLAFATSRISFIQLFGIGTALAIVVDATLIRALLVPA
                     FMRLAGRANWWAPGPLR"
BASE COUNT          178 a          717 c          576 g          278 t
ORIGIN      
        1 gccgccctcg ccgccgcata cagcggcgag ggcgccgcga ccagggtgca ggtcggcggg
       61 cagctcatgt tcaaccacga cgtcggccgg caggtccagc gggacctggc caaggcggaa
      121 ggcatctccg tcccgatcac cctgctgctg ctcgtggtgg tgttcgcgag cgcggtggcc
      181 ggcgctctgc cgctcgccgt cggcggcttc gcgatcgtcg gcaccctgct ggtgctccgc
      241 gtcctggccg gcgtcaccga cgtgtcgatc tactccctca acctcaccac cgcgctgggg
      301 ctcagcctcg ccatcgacta cagcctgttc atcgtctccc gctaccggga ggagctgcgc
      361 cccggccgcg cgcccggcga cgccctggtc gcgaccatgc gcaccgccgg caagacggtg
      421 ctgttcagcg cggccacggt ggccgtctcc ctgactgccc tgctcgtctt cccgctctac
      481 ttcctgcgct cgttctgcta cgccgggatc gcggtcgtcg cgctggccgc gacgggcgcc
      541 gtcgtcgtcc tcccggccct gctcgccctc atcggccccc gggtgaaccg cctgcggctg
      601 cccatcggcc ggcgccgccc ggccgccgct cccggcgccc cgggcgaggt gggcgggaac
      661 ggcgcctggc accggatcgc ggtcacggtg atgcggcggc cgctgccggt cgcgctcacc
      721 gtcatcgccc tcctcgtcct gctcggcgcc ccgttcctgc acgtgcgctt cggcctgccc
      781 gacgaacggg tgcagcccac ctccgccccg agccggcagg tctccgacgc catccgccgc
      841 ggcttcgcct ccgacgacgc cgccgcgctg tcggtggtga ccaccggcgc cgaggggccg
      901 gccggccacg ccgccgacat cgaccgctac gccggcgacc tgtccgccct cgccggggtg
      961 gccagggtcg acgccctgac cgggacgtac accggcggcc ggcgggtcac cggcccgggg
     1021 cccgcctccg gccgcttcgc cgccccgacc gcaacctggc tctcggtcgt cccctcggtc
     1081 gacgccaact ccccggaggg cgaggccgtc gtccaccggg tgcgcgacct gcccgcaccg
     1141 ttcgcggtca aggtcaccgg cccgtcggcc cagctcgtcg acaccaaggc cgccctgttc
     1201 ggccgcctgc cggccgccgc cgcgatcatc gccgcggtga cgttcgtggt gctgttcctc
     1261 ttcaccggca gcgtcgtcat cccggccaag gccctggtgc tgaacctgct ctcgctcacc
     1321 gccgtctacg gcgccatggt ctggatcttc caggagggcc acctggcggg cttcctcggg
     1381 ttcaccgcca ccggtaccat cgacacctcc atgccggtgc tgctgttctg catcgccttc
     1441 ggcctgtcga tggactacga ggtgttcctg ttgtcgagga tcaaggagga gcacgaccgc
     1501 accggcgaca ccgtgaccgc ggtcgccgtc gggctcgagc gggccggccg catcgtgacc
     1561 gccgccgccg cgctgctcac catcgtgttc ctggcgttcg ccacctcgag gatcagcttc
     1621 atccagctct tcgggatcgg caccgcgctc gcgatcgtcg tggacgccac cctcatccgg
     1681 gcgctgctcg tgcccgcctt catgcgcctg gccggccggg cgaactggtg ggcgcccggc
     1741 ccgctgcgt
//
LOCUS       DASPYY010000609         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2210081, whole genome shotgun sequence.
ACCESSION   DASPYY010000609 DASPYY010000000
VERSION     DASPYY010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210081"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1442)
                     /locus_tag="VEP73_09590"
     CDS             complement(273..1442)
                     /locus_tag="VEP73_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYT26719.1"
                     /translation="MSRSLRGAASVAGIGATPYYKRGTSPHSALRLTLEAILAACADA
                     GLDAHDVDGFVSYANDVSEGLAVGAALGVREIRWSTMVWGGGGGGVAAAVNAAAAAVA
                     SGQAEHVIVYRGLTEADDGRQSYGKGHFPPLLSAHGVLTPAQVCALRTQRMLEVDGVP
                     GSALEALVQASYHHAQQNPDAVAYGRPLDHDTYAGARWISEPLRLFDCSRENDGAAAV
                     LVTSAERARDLRPVPAYVLSGVQGAGPDWSESAENNAAYTSAGFHPALVARLWTEAGV
                     GPEETDVVQVYENFSGPAVASLVDLGLCPPGADAGSFMTLPNLIAPTGTLPVNTAGGN
                     LAEGFVHGVGLVLEAVRQIRGTSANQVPGADISLLLGGPVAPLVSATVFGSAASL"
BASE COUNT          210 a          552 c          549 g          190 t
ORIGIN      
        1 gcctactgag cgtcggatac gccaggctcc gcaggctttc gccgaagcca tcatcgacgc
       61 cgacagctac tgaccgggct gcgcgaatcg ccaaccgtta gggaacagcc ggacctgttc
      121 gccgccgaaa tgcgggatgc gttccggaca ctgcgctagc cgggcagagg cggacggcgc
      181 tcggcctggg cgccgtccgc ccggggccgg tcaggatcgg cagggaagag ctcacccgcg
      241 cgaacaccag cctgcggtta acggtcggct ggtcacaggc tcgccgccga gccgaagacg
      301 gtcgcgctga cgagcggcgc gaccgggccg ccgagcagca gcgagatgtc cgcgccaggg
      361 acctggttgg ccgaggtgcc gcggatctgc cggaccgcct cgaggaccag cccgaccccg
      421 tggacgaagc cctcggcgag gttcccgccc gcggtgttga ccgggagcgt gcccgtcggc
      481 gcgatgaggt tgggcagggt catgaacgat cccgcgtccg cgcccggtgg gcacagcccg
      541 agatcgacca gggacgccac ggccgggccg ctgaagttct cgtacacctg gacgacatcg
      601 gtctcctcag gcccgacgcc cgcctcggtc cagagccggg cgaccagggc cgggtggaag
      661 cccgcgctgg tgtaggcggc gttgttctcg gccgactcgc tccagtccgg cccggcgccc
      721 tgcacgccgg acagcacgta ggccggtacc ggacgcaggt ccctggcccg ttcggccgac
      781 gtgaccagga ccgcggcggc tccgtcgttc tcccgggagc agtcgaacag gcgcagcggc
      841 tcggagatcc accgggcccc ggcgtaggtg tcgtggtcga gggggcggcc gtaggccacg
      901 gcgtccgggt tctgctgcgc gtggtggtag gacgcctgga ccagcgcctc cagcgcggac
      961 ccgggtaccc cgtcgacctc gagcatccgc tgggtccgca gcgcgcagac ctgagccggc
     1021 gtgagcaccc cgtgggcgga cagcagtggc gggaagtgtc ccttcccgta actctgccgc
     1081 ccgtcgtcgg cctcggtgag cccgcggtac acgatgacgt gctcggcctg gccgctggcc
     1141 accgccgcgg ccgccgcgtt gaccgccgcg gcgacgccgc cgcctccgcc gccccacacc
     1201 atggtcgacc agcggatctc gcggacgcct agggccgcgc cgaccgcgag gccctcgctc
     1261 acgtcgttgg cgtaggagac gaagccgtcg acgtcgtggg cgtcgagccc ggcgtcggcg
     1321 cacgcggcca ggatcgcttc cagcgtcagc cgcagcgcgg agtgcgggct cgttccgcgc
     1381 ttgtagtacg gggtggcgcc gatccccgcc accgaggccg ccccgcgaag cgaccggctc
     1441 acaccagccg ccagcgcagt acgggccagc cgtcaggatc gggagccgga tcgatctcgc
     1501 c
//
LOCUS       DASPYY010000610         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_805569, whole genome shotgun sequence.
ACCESSION   DASPYY010000610 DASPYY010000000
VERSION     DASPYY010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805569"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..893
                     /locus_tag="VEP73_09595"
     CDS             18..893
                     /locus_tag="VEP73_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26720.1"
                     /translation="MLLTELALVADTNQVTCPQLNQVAAALQKQAIRDFRPLWRVTAT
                     VDGFERLEDVPVGYWPIIVRDDIDMPGAAGVHLDQNGQPFALVQYSDSWSLTASHEML
                     EMLVDPWGNRLVAGQSQKPDQGRVEFLVEIADPSEDAQFGYTVNGLLVSDFLTPHFYD
                     PVAVAGVRYSFGGHIPGPRQIVQGGYISWHDPVSDHWWQQTWFGGPAPTFRDLGKLTA
                     RAGSLRSAIDARTDTARRIAQSGPRSERFAEVTAFRPRVKESAAARAVGWRDQIEALK
                     SGQLTEASWEGGEEG"
     gene            925..1152
                     /locus_tag="VEP73_09600"
     CDS             925..1152
                     /locus_tag="VEP73_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26721.1"
                     /translation="MDEDEDKNEDDEDENGERKAREARAARLREEIERLKRGGPATAD
                     PEAPSGPESPREFIERRMRELDTPDPREPGD"
     gene            complement(1213..1971)
                     /locus_tag="VEP73_09605"
     CDS             complement(1213..1971)
                     /locus_tag="VEP73_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HYT26722.1"
                     /translation="MSTSGPLQVPPLPVPDGELQVTDPEEIADTLHDLARLDPVEVGL
                     ETVLDRIVRAAVACCGVTGGVGLLLLDSADELRHVVAAGRPLRSLGGMQVRLREGPSM
                     DAFQRDEPVDSGDLTAEPRWPAFGPVAVAAGVRAWLAVPVHRRYGPIGTLDFARTEPQ
                     PWRPQDLAAGNAYARLIVSTLELAMAHGEEGLASELRFALDHDARIEQARGVVMEREQ
                     VDEPSAFELLRQQAYIEGQPMRAIAERILARARR"
     gene            complement(2041..3231)
                     /gene="ugpC"
                     /locus_tag="VEP73_09610"
     CDS             complement(2041..3231)
                     /gene="ugpC"
                     /locus_tag="VEP73_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYT26723.1"
                     /translation="MGSIVLEHVSKEFPGGVQAVRDVSLEIEDGEFMVLVGPSGCGKT
                     TILRMIAGLDEVTGGEISIGDRVVTDEKPKDRDIAMVFQNYALYPHMTVEQNIGFGMR
                     LRRTPKTEMKQRVGGAAQVLALLDYLARKPADLSGGQRQRVAIGRAMVREPKAFLMDE
                     PLSNLDAKLRVQMRTELARLHRQLRTTIVYVTHDQIEAMTLGTRVAVVRDGVLQQVDT
                     PQMLFHNPVNLFVAGFIGSPAMNLVEATVAGDELAFGSHRLPVPPEAALDGYEGRSVI
                     LGIRPSDFQDADAWRDERLPVVEVTADVVEDLGSETHVLFTVEAPPVQVEGVAEDPEA
                     GTPIPLVAPEGTSVFTAAVDARTSARPGGRVRLSVDPARFHFFDRETGRVIRSRPLEV
                     AAST"
     gene            complement(3243..>4192)
                     /locus_tag="VEP73_09615"
     CDS             complement(3243..>4192)
                     /locus_tag="VEP73_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HYT26724.1"
                     /translation="VLGAARGPGASHQHLGVVGAMAALEALVVAACRDAGLDPGVRPL
                     AEVGVWCLAGLDLPADDEALGPAIAGCGWARENLLHNDVFAVLRAGSERTWGVGVVAG
                     AGMNCAGVAPDGTEARFPALGELSGDWGGGRSIGVAAVGAALRGEDGRGPRTALERLV
                     PGHFGLPSALAVVEAVYLGRIAEDRVLELPPLVFAAAAAGDPVAAELVGRQVAEAATM
                     AGAAIRRLGLTGRDVDVVLGGGLFHRDDPAFIERIRAGIAKVAPAARLRQVTAPPVVG
                     AALLGLDRLGAAPGAAGRLRASLTHERLQALQPVAASTH"
BASE COUNT          572 a         1564 c         1558 g          498 t
ORIGIN      
        1 gcgacgaagg aggccccgtg ctgctgaccg agctcgctct cgtagccgac accaaccagg
       61 tcacgtgccc ccagctgaac caggtggccg ccgcgctgca gaagcaggcc atccgggact
      121 tccgccccct gtggagggtc acggcgaccg tggacggctt cgagcggctc gaggacgtgc
      181 ccgtcgggta ctggccgatc atcgtccggg acgacatcga catgcccggg gccgcgggcg
      241 tccacctcga ccagaacggc cagccgttcg cgctcgtgca gtacagcgac agctggtccc
      301 tcacggccag ccacgagatg ctcgagatgc tggtcgaccc gtggggcaac cggctggtgg
      361 ccggccagtc gcaaaagccc gaccagggcc gggtggagtt cctggtcgag atcgccgacc
      421 cctcggagga cgcgcagttc ggctacacgg tgaacggcct gctcgtctcg gacttcctca
      481 ccccccactt ctacgacccg gtggcggtcg ccggggtgcg ctacagcttc ggcgggcaca
      541 tccccggtcc caggcagatc gtccagggcg gctacatcag ctggcacgac ccggtgagcg
      601 accactggtg gcagcagacc tggttcggcg gccccgcgcc cacgttccgc gacctcggca
      661 agctgaccgc gcgggccggc agcctccggt cggccatcga cgcccgtacc gacaccgcgc
      721 ggcgcatcgc gcagagcggt ccgcggtcgg agcggttcgc cgaggtgacg gcgttccggc
      781 cgcgcgtcaa ggagtcggcc gccgcgcgcg ccgttggctg gcgcgaccag atcgaggcac
      841 tcaagagcgg tcagctgacc gaggcgtcgt gggagggcgg cgaggagggg tgatccccac
      901 cggagcggag ccggaggcga cggggtggac gaagacgagg acaagaacga ggacgacgag
      961 gacgagaacg gggagcggaa ggcgcgggag gcccgcgcgg cccggctccg ggaggagatc
     1021 gagcggctga agcgaggcgg accggccaca gcggacccgg aagcgccctc cggaccggag
     1081 agcccgcgcg agttcatcga gcgccggatg cgggagctcg acacgccgga cccccgcgag
     1141 ccgggcgact gagcgctgcc cgcggccccg ccgcccgagg acgacctcga ggacgacccg
     1201 gtgcgccctt ccctaccgtc tggcgcgggc gaggatgcgc tcggcgatgg cccgcatcgg
     1261 ctggccctcg atgtaggcct gctggcgcag cagctcgaac gcggacggct cgtcgacctg
     1321 ctcgcgctcc atgacgacgc cgcgcgcctg ctcgatccgg gcgtcgtggt ccagcgcgaa
     1381 ccgcagctcg gaggcgagcc cctcctcgcc gtgggccatg gccagctcca gggtcgagac
     1441 gatcagccgg gcgtaggcgt tgccggcggc gaggtcctgg ggccgccacg gctggggctc
     1501 ggtgcgggcg aagtcgagcg tcccgatggg gccgtagcgg cggtgcaccg gcacggccag
     1561 ccacgccctg acgcccgccg cgaccgccac cggcccgaac gccggccagc gtggctcggc
     1621 ggtgaggtcg ccgctgtcga ccggctcgtc ccgctggaag gcgtccatgg acgggccctc
     1681 gcgcagccgg acctgcatgc cgccgaggga ccgcagcggc ctgcccgcgg cgacgacgtg
     1741 gcgcagctcg tcggccgagt cgagcaggag caggcccacg cccccggtga ccccgcagca
     1801 ggccaccgcg gcgcggacga tccggtcgag gacggtctcg aggccgacct cgaccgggtc
     1861 caggcgcgcg aggtcgtgga gcgtgtcggc gatctcctca gggtcggtga cctggagctc
     1921 gccgtcggga accggcagcg gggggacttg caacggtcct gatgtgctca tggtcgagag
     1981 tccttcccca ttcaccgcga tcctgaacat gggaccccgg atcggcgtcg ccgcgcgggc
     2041 tcaggtggag gccgccacct cgagcgggcg gctgcggatc acccggccgg tctcgcggtc
     2101 gaagaagtgg aagcgggcgg ggtccaccga gaggcggacg cgcccgcccg ggcgggcgct
     2161 ggtgcgggcg tcgaccgcgg ccgtgaacac gctggtcccc tccggcgcga cgagcgggat
     2221 cggcgtgccg gcctccgggt cctccgcgac gccctcgacc tgcaccggcg gcgcctcgac
     2281 cgtgaacagc acgtgcgtct cggagccgag gtcctcgacc acgtccgcgg tcacctcgac
     2341 gaccgggagc cgctcgtcgc gccaggcgtc ggcgtcctgg aagtccgacg ggcggatccc
     2401 gaggatcacc gaccgcccct cgtacccgtc cagcgccgcc tcgggcggga cgggcaggcg
     2461 gtggctgccg aaggcgagct cgtccccggc gacggtggcc tcgaccaggt tcatcgccgg
     2521 cgagccgatg aacccggcga cgaacaggtt gaccgggttg tggaagagca tctgcggcgt
     2581 gtccacctgc tgcagcacgc cgtcgcggac caccgcgacc cgggtcccga gcgtcatcgc
     2641 ctcgatctgg tcgtgggtga cgtacacgat cgtggtgcgg agctgcctgt ggagccgcgc
     2701 cagctcggtg cgcatctgca cccgcagctt ggcgtcgagg ttggagagcg gctcgtccat
     2761 gaggaacgcc ttcggctcgc gcaccatcgc ccgcccgatc gccacccgct ggcgctggcc
     2821 gccggacagg tcggccggct tgcgcgccag gtagtcgagc agcgcgagga cctgcgccgc
     2881 cccgcccacg cgctgcttca tctcggtctt cggcgtgcgg cggaggcgca tgccgaagcc
     2941 gatgttctgc tcgacggtca tgtgcgggta cagggcgtag ttctggaaga ccatcgcgat
     3001 gtcgcggtcc ttgggcttct cgtcggtgac cacccggtcg ccgatgctga tctcgccgcc
     3061 ggtgacctcg tcgaggccgg cgatcatgcg caggatcgtg gtcttgccgc agccggacgg
     3121 gcccaccagc accatgaact cgccgtcctc gatctccagc gacacgtcgc gcacggcctg
     3181 cacgccgccg gggaactcct tgctcacgtg ctcaagaacg atgctgccca tcgatgccct
     3241 cctcagtgtg tcgaggccgc gaccggttgg agtgcttgca gacgctcgtg ggtgaggctc
     3301 gcgcggaggc gcccggcggc gccaggggcc gcgccgagcc ggtcgaggcc gagcagggcg
     3361 gccccgacca ccggcggcgc ggtgacctgc ctgagcctgg ccgccggcgc gaccttcgcg
     3421 atcccggcgc ggatccgctc gatgaaggcg gggtcgtcgc ggtggaacag gccgccgccg
     3481 aggaccacgt cgacgtcccg gccggtgagg ccgagccgcc ggatcgccgc cccggccatg
     3541 gtcgcggcct cggcgacctg ccgcccgacg agctccgcgg cgaccgggtc gccggccgcc
     3601 gccgccgcga acaccagcgg gggcagctcc agcacccggt cctcggcgat ccgcccgagg
     3661 tagaccgcct ccaccaccgc gagcgccgag ggcagcccga agtgcccggg gaccagccgc
     3721 tcgagcgccg tccgcggccc gcggccgtcc tcgccgcgca gggcggcgcc caccgccgcg
     3781 acgccgatgc tgcggccgcc gccccagtcg ccggagagct cgccgagcgc ggggaagcgc
     3841 gcctcggtgc cgtcgggcgc gacgcccgcg cagttcatgc cggccccggc caccacgccg
     3901 accccccagg tccgctcgct gcccgcgcgc agcaccgcga agacgtcgtt gtggaggagg
     3961 ttctcgcgcg cccagccgca cccggcgatc gcgggcccga gcgcctcgtc gtcggccggc
     4021 aggtccaggc ccgcgaggca ccagacgccg acctcggcca gcgggcgcac cccggggtcg
     4081 aggccggcgt cccggcaggc agccacgacc agcgcctcga gcgcggccat cgcgcccacg
     4141 acgccgaggt gctggtgcga cgcgcccggc ccgcgggccg ccccgagcac gg
//
LOCUS       DASPYY010000611         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1648897, whole genome shotgun sequence.
ACCESSION   DASPYY010000611 DASPYY010000000
VERSION     DASPYY010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648897"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VEP73_09620"
     CDS             <1..927
                     /locus_tag="VEP73_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26725.1"
                     /translation="ARMAYLLALGEDRGAWREGWRRALLDELLRVVLAAMVEPGLTDH
                     VAERLVHRRRAEVELAIDGAGPGAAALLAAAPRRYLLGQPAEAIARHLAMAEPAPARR
                     ELRLAISPEPAGGRGGAWRVDAVTRDRPGLLARLVGALSACGLDITRAQASTWPDDLV
                     VDVFHVAGPAPEDEPGLPDRVERMLRRAMEGDLDPVRLLAERPDGNRAGPVVVRVDAG
                     ASPWHSTVWVEAPDRPGLLYEILLELARHHVDVQAARVGDGAGMAADLFLVTDAAGNP
                     LEREAADRLGTALAGRLTDPAGRQRAGGQVRR"
     gene            complement(912..>1858)
                     /locus_tag="VEP73_09625"
     CDS             complement(912..>1858)
                     /locus_tag="VEP73_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYT26726.1"
                     /translation="KTGRVRAMRDGRIDPTPVLDLSSEVSGGAEQGLLGLAFSLDRRF
                     LYVYYTDLGGDIHVVEFAMRGGRADPGSRRELLRIEHSRFGNHDGGQLVMARDGTLWA
                     GVGDGGGAGDPFGSGQNLDTLLGKILRISPRPSGGRPYGIAAGNPFVRRSGARPEIWA
                     YGLRNPWRFSFDPANGDLWIGDVGQGMWEEVDHEPAGSGGGRNYGWNRREGLHQFSGR
                     RPAGAVDPVLEYGHDGGACSVTGGYVYRGSEVPELRGSYVYGDYCAGWIRAVQLRDGK
                     VAARRDLGLNLPGLSSFGTDQAGELYALSLNGGVYRLT"
BASE COUNT          216 a          723 c          701 g          218 t
ORIGIN      
        1 gcgcgcatgg cctacctgct cgccctcggg gaggaccggg gcgcctggcg ggagggctgg
       61 cggcgggccc tgctcgacga gctgctccgg gtcgtcctcg cagccatggt ggagcccggc
      121 ctcaccgacc acgtggccga gcgcctcgtc caccggcgcc gcgccgaggt cgagctggcc
      181 atcgacggcg ccggtcccgg tgcggcggcc ctgctggccg ccgcaccccg ccgctacctg
      241 ctcggccagc ccgccgaggc gatcgccagg cacctcgcca tggccgagcc ggcccccgcc
      301 cggcgcgagc tgcgcctcgc gatctcgccc gagccggccg gcggccgtgg cggcgcctgg
      361 cgcgtcgacg ccgtcacgcg cgaccgtcca gggctgctgg cgaggctggt cggggcgctg
      421 tccgcctgcg gcctggacat cacccgggcg caggcgtcga cctggccgga cgacctcgtc
      481 gtggacgtgt tccacgtcgc cggcccggcg cccgaggacg agccgggcct cccggaccgg
      541 gtcgagcgga tgctccgccg ggcgatggag ggcgacctgg acccggtcag gctgctcgcc
      601 gagcggcccg acgggaaccg cgccggcccg gtcgtcgtcc gggtcgacgc cggcgcgtcg
      661 ccgtggcact cgaccgtgtg ggtggaggcg cccgaccggc ccggcctcct ctacgagatc
      721 ctgctcgagc tggcccgcca ccacgtcgac gtccaggcgg cacgcgtcgg ggacggggcg
      781 ggcatggccg ccgacctgtt cctcgtcacc gacgcggccg gtaaccccct cgagcgggag
      841 gccgccgacc gtctcggcac cgccctcgcc ggccgcctga ccgacccggc gggccgccag
      901 cgggctggcg gtcaggtgag gcggtagacg ccgccgttca gggagagggc atagagctca
      961 ccggcctggt cggtcccgaa cgaggagagc ccggggaggt tgaggccgag gtcccgccgc
     1021 gcggcgacct tgccgtcccg cagctgcacg gctctgatcc acccggcgca gtagtcaccg
     1081 tagacgtagg agccgcgcag ctcggggacc tccgacccgc ggtagacgta gccgccggtc
     1141 accgagcagg cgccgccgtc gtggccgtac tcgaggaccg ggtcgaccgc cccggccggc
     1201 cgccggccgg agaactggtg gagcccctcg cgccggttcc agccgtagtt gcggccgccg
     1261 cccgacccgg ccggctcgtg gtccacctcc tcccacatcc cctgccccac gtcgccgatc
     1321 cagaggtcgc cgttggcggg gtcgaacgag aagcgccagg ggttgcgcag cccgtacgcc
     1381 cagatctcgg gcctggcgcc cgagcgccgg acgaaggggt tgcccgccgc gatgccgtac
     1441 gggcggccgc ccgacgggcg gggggagatc cgtagaatct tgccgagcag cgtgtcgagg
     1501 ttctggccgc tgccgaacgg gtcgccggcc ccgccgccgt cgccgacccc ggcccagagg
     1561 gtcccgtcgc gcgccatcac cagctggccg ccgtcgtggt tgccgaaccg gctgtgctcg
     1621 atccggagca gctcgcggcg ggacccgggg tcggcccggc cgccgcgcat cgcgaactcg
     1681 accacgtgga tgtcgccgcc caggtcggtg tagtagacgt agaggaaccg ccggtcgagc
     1741 gagaacgcca ggccgagcag cccctgctcg gcgccgcccg agacctccga ggacaggtcg
     1801 agcacggggg tcgggtcgat gcggccgtcc cgcatggcgc gcacccggcc ggtcttct
//
LOCUS       DASPYY010000612         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1087202, whole genome shotgun sequence.
ACCESSION   DASPYY010000612 DASPYY010000000
VERSION     DASPYY010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087202"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VEP73_09630"
     CDS             complement(<1..1045)
                     /locus_tag="VEP73_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT26727.1"
                     /translation="MRKLQNFVGGEYRDAADGRTTTLVNPSTGEGFAEAPLSGSGDVE
                     AACQAAVRGFEEWRDATPAERSRALQRIADAMEDRGEELVRLECENTGKPFQLTMEEE
                     LPPMVDQIRFFAGAARVLEGRSAGEYMAGHTSMVRREPVGVCAQVTPWNYPAMMAVWK
                     WAPAIAAGNAVVLKPSDTTPVTTVWMAGLMSEFLPPGVVNVICGDRGTGAALVSHKVP
                     AMVSITGSVRAGREVARAAADDLKRVHLELGGKAPVIVFDDADVAAAAEAIAAAGYFN
                     AGQDCTAATRVLAGPGVHDDFLAALVEQARQTKTGAPDEDVLYGPLNNASQLARVEGF
                     VDRAPDHAEVVAGG"
     gene            complement(1090..>1775)
                     /locus_tag="VEP73_09635"
     CDS             complement(1090..>1775)
                     /locus_tag="VEP73_09635"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT26728.1"
                     /translation="DTKSKLNLRWQGFTFFWYRNVFSIDALSAALRNTLLIAVLSTAI
                     STVLGTMIGLALGRYRFRGRGGVDLVIFASISAPELVLGAALLSLFITLQVPRGLLTI
                     LIAHVMFSVSFVAVTVRARMAGFDVTLEEAAQDLGADPWTTFRKVTLPLIFPGVMAGA
                     LLAFALSIDDYIITSFVNGQTETFPIWVYGATRQGVPPQVNVMGTFLFLTGVGLAVAN
                     VVALRRRRL"
BASE COUNT          250 a          627 c          651 g          247 t
ORIGIN      
        1 ggccgccggc caccacctcg gcgtggtccg gggcgcggtc cacgaagccc tcgacgcggg
       61 cgagctggct cgcgttgttg agcgggccgt acagcacgtc ctcgtcgggc gcgccggtct
      121 tcgtctgcct ggcctgctcg acgagggcgg cgaggaagtc gtcgtgcacc ccgggcccgg
      181 cgagcaccct ggtcgcggcg gtgcagtcct ggccggcgtt gaagtacccg gcggccgcga
      241 tcgcctcggc ggcggcggcg acgtcggcgt cgtcgaacac gatcaccggc gccttgccgc
      301 cgagctccag gtggacccgc ttcaggtcgt cggcggccgc gcgcgcgacc tccctgccgg
      361 cccggacgct gccggtgatg ctcaccatcg ccgggacctt gtggctgacc agcgcggcgc
      421 cggtcccgcg gtcgccgcag atcacgttga cgacgccggg cggcaggaac tcgctcatca
      481 gcccggccat ccacaccgtc gtcacggggg tggtgtcgga cggcttgagg acgaccgcgt
      541 tgccggccgc gatggccggg gcccacttcc acacggccat catcgccggg tagttccagg
      601 gggtgacctg ggcgcagacg ccgaccggct cccgccggac catggacgtg tggccggcca
      661 tgtactcccc tgcagagcgc ccctccagca cgcgggccgc gccggcgaag aacctgatct
      721 ggtcgaccat gggggggagc tcctcctcca tggtgagctg gaacggcttg ccggtgttct
      781 cgcactcgag ccggaccagc tcctcgccgc ggtcctccat ggcgtcggcg atgcgctgca
      841 gggcgcggct gcgctcggcc ggggtcgcgt cgcgccactc ctcgaacccg cgcacggccg
      901 cctggcaggc ggcctcgacg tcgcccgagc cggacagcgg cgcctcggcg aagccctccc
      961 cggtcgacgg gttgaccagc gtcgtggtgc ggccgtcggc cgcgtcgcgg tactcgccgc
     1021 cgacgaagtt ctgcagcttg cgcatgttcc gtgctcctcc tggccgaggt cgggccgtgc
     1081 cggtgcggtt cagagcctgc ggcgccgcag ggcgacgacg ttggccaccg ccagcccgac
     1141 gccggtcagg aacaggaagg tccccatcac gttgacctgg ggaggcacgc cctggcgggt
     1201 ggcaccgtag acccagatcg ggaacgtctc cgtctggccg ttgacgaagc tcgtgatgat
     1261 gtagtcgtcg atggacaggg cgaacgcgag cagcgccccg gccatgacgc ccgggaagat
     1321 gagcggcagc gtgaccttgc ggaacgtcgt ccaggggtcg gcgccgaggt cctgggcggc
     1381 ctcctcgagc gtcacgtcga acccggccat cctggcacgg acggtgaccg ccacgaacga
     1441 cacgctgaac atcacgtggg cgatcaggat ggtgaggagc ccgcgcggca cctgcagggt
     1501 gatgaacagg ctgagcaggg ccgcgccgag caccagctcg ggcgccgaga tggacgcgaa
     1561 gatgacgagg tcgaccccgc cccgcccccg gaaccggtag cggccgagcg cgaggccgat
     1621 catcgtgccg agcacggtcg agatggccgt cgacagcacg gcgatcagca gcgtgttcct
     1681 gagcgccgcg gagagcgcgt cgatcgagaa gacgttgcgg taccagaaga acgtgaagcc
     1741 ctgccagcgc aggttcagct tgctcttggt gtcgt
//
LOCUS       DASPYY010000613         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_244368, whole genome shotgun sequence.
ACCESSION   DASPYY010000613 DASPYY010000000
VERSION     DASPYY010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244368"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VEP73_09640"
     CDS             complement(<1..208)
                     /locus_tag="VEP73_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26729.1"
                     /translation="MTGLDELRATPQDEQVWQDVVVVVAVYAVLAGWTVAGVPQRTVE
                     NASFVGAVLGGALYAALRVQARESG"
     gene            complement(378..>2006)
                     /locus_tag="VEP73_09645"
     CDS             complement(378..>2006)
                     /locus_tag="VEP73_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYT26730.1"
                     /translation="PAGRSRSSRRRYHPASATLETTWDTGTGRLVLTEGMVAEVTGRL
                     LPTTLLVRRLAAVSGPVDAVVELDPRLGERHRPPEARRRGAALVCQWGSIAVAVQAAP
                     WLPLEPGRPLRVRVAPGQPLTVVLAVASREPLVHVDPDAAWMALLDDERRWRAWSGDV
                     DEDLPERDAVVRSLLTLRLLTYAPSGAPVAAPTTSLPEHPGGARNWDYRFAWPRDASI
                     GIGAFLGVGKTVEARQFMAWLLHATRLERPRLPVLLTLHGKHPPAERELQGWPGYAGS
                     RPVRFGNGAAEQHQLDGYGWVLDAAWLLTRAGQRLYIETWRAMRGFADRVAQRWPEPD
                     AGIWEIRGDAAHHVHSKLMAWLALDRALRIAAELGVSHRRRGWWQAQRDAIAADVTRR
                     GFNTGKAAYTRTYGSEDLDAAVLVLPLLGIEPLDSPRVRGTLDAIGRELGASGPLLYR
                     YPPGEDGLPGTEGAFLPCSFWLVQALAKTGRHAEAAERFAEMLALASPLGLYAEQMDP
                     TSRQHLGNYPQALTHAALVQAALALRDAERPRGL"
BASE COUNT          270 a          721 c          773 g          242 t
ORIGIN      
        1 atcccgattc cctcgcctga acgcgaaggg cggcgtagag cgctcctccg aggaccgcac
       61 cgacgaagct cgcgttctcc acggtcctct ggggcacacc ggccaccgtc cagccggcca
      121 gcaccgcata caccgccaca acgacgacga catcctgcca gacctgctcg tcctgagggg
      181 tcgcgcgtaa ctcgtccagc ccggtcaacc gagcgacgag gacgtttccg aacctgaagg
      241 cggcgagccc tgtggtgata gggcggcgct gagccatacg tgccgccaca cccagattgt
      301 cttgtgcggg gacgccatcg cggatgcagc ctccggtccg ctcctcgcga acgaggatag
      361 ccgggaccgg cggctgccta cagcccccgg ggccgctcgg catccctgag cgcgagcgcg
      421 gcctggacca gcgcggcgtg ggtgagggcc tgcgggtagt tgccgaggtg ctgccgactg
      481 gtcgggtcca tctgctcggc gtacaggccg agggggctgg cgagggccag catctcggcg
      541 aaccgctcgg cggcctcggc gtgccggccg gtcttcgcca gcgcctggac gagccagaac
      601 gagcacggaa ggaaggctcc ctcggtgccg ggaagcccgt cttcgccggg cgggtagcgg
      661 tacagcaagg gcccggaggc gcccagctcg cggccgatgg cgtcgagggt gccgcggacc
      721 cggggcgagt cgagcggctc gatgccgagc agcggcagca ccagcacggc ggcgtcgagg
      781 tcctcggagc cgtaggtgcg ggtatatgcg gccttgccgg tgttgaagcc tcgcctggtg
      841 acgtcggcgg cgatggcgtc ccgctgggcc tgccaccagc cgcgccggcg gtgggacacc
      901 cccagctcgg ccgcgatgcg gagcgcccgg tccagggcga gccaggccat gagcttggag
      961 tggacgtggt gggccgcgtc gccgcggatc tcccagatgc cggcgtcggg ctcgggccag
     1021 cgctgggcga cgcggtcggc gaagccgcgc atggcccgcc acgtctcgat gtagaggcgc
     1081 tgcccggccc gggtgagcag ccaggcggcg tcgagcaccc agccgtagcc gtccagctga
     1141 tgctgctcgg ccgccccgtt gccgaaccgc accgggcggc tgccggcata gcccggccag
     1201 ccctggagct cgcgctcggc cggcgggtgc ttgccatgca gggtcagcag gacgggcagc
     1261 cggggccgct cgagccgggt ggcgtgaagc agccaggcca tgaactggcg ggcctcgacg
     1321 gtcttgccga cgccgaggaa ggcaccgatg ccgatgctgg cgtcccgggg ccatgcgaag
     1381 cggtagtccc agttgcgggc accgccgggg tgctcgggca gcgacgtggt cggggcggcg
     1441 accggcgccc cggagggcgc gtaggtgagc agccgcagcg tcagcaggct gcgcaccacc
     1501 gcgtcgcgct cgggcaggtc ctcgtcgacg tcgccgctcc atgcccgcca gcggcgctcg
     1561 tcgtcgagca gggccatcca cgccgcatcg ggatcgacgt ggaccagcgg ctcacggctg
     1621 gcgacggcga gcacgacggt gagcggctgg cccggcgcga ccctgacccg cagagggcgt
     1681 cccggctcga gcggcagcca gggggcggcc tggacggcga ccgcgatcga tccccactgg
     1741 cagaccagcg cggcgccgcg ccgtcgggcc tcggggggcc ggtgccgttc cccgaggcgc
     1801 gggtcgagct cgacgacggc atcgaccggg ccgctcaccg ccgcgaggcg ccggaccagc
     1861 agcgtggtcg gcagcagcct gccggtgacc tcggccacca tgccctcggt cagcaccagg
     1921 cgcccggtcc cggtgtccca ggtcgtctcc agggtcgccg acgccgggtg gtagcgccgg
     1981 cggctcgacc gggaccgccc ggcggg
//
LOCUS       DASPYY010000614         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1368306, whole genome shotgun sequence.
ACCESSION   DASPYY010000614 DASPYY010000000
VERSION     DASPYY010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368306"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..637)
                     /locus_tag="VEP73_09650"
     CDS             complement(38..637)
                     /locus_tag="VEP73_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HYT26731.1"
                     /translation="MSTPAVTLFLALLTVGAQLALAVALVLAVTGRWWPAAARLRAEL
                     AAAIRPQALQLAFLVALVAMLGSLYLSEVAHFTPCKLCWYQRAAMYPLALVLGVAAWR
                     RDLAVRPYGLALALVGAPISAYHYLLERVPTLESGTCDPDNPCTLLWVWRFHYISIPL
                     MALSAFALVAVLLLAARPGPRPDRPGARDLGTLASADRA"
     gene            1092..1358
                     /locus_tag="VEP73_09655"
     CDS             1092..1358
                     /locus_tag="VEP73_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26732.1"
                     /translation="MWGRTDPAEIVRQRLRHGVDRLRRAHHGHHGEYDPSVSAQLPYG
                     EGLSPSALVELVEGSGWARPASSGSVTSSGPACSPAHRSSGSSA"
     gene            complement(1366..2760)
                     /locus_tag="VEP73_09660"
     CDS             complement(1366..2760)
                     /locus_tag="VEP73_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HYT26733.1"
                     /translation="MSRTITGVEVFDVRFPTSRSLDGSDAMNPDPDYSAAYCILHTSA
                     GDGLEGHGFTFTIGRGNEVVAAAIRALAPLVVGEDADRFFADMGAFWRRVTSDSQLRW
                     IGPEKGAIHLATAAVVNATWDLYAKVEGKPLWRLLAELSPEDLVGCVDFRYITDALTP
                     GEALEILRAAEPARGRRAEAMLERGYPAYTTSAGWLGYPDDKLRRLCREAVGEGWTHV
                     KIKVGRDTDDDVRRAGIVRAEIGPDRKLMMDANQIWDVGEAIAAMGRLAEFDPWWIEE
                     PTSPDDVLGHAAIARAIAPIGVATGEHCQNRVVFKQLLQARAIRFCQVDACRLGGVNE
                     VLAVLLMAAKFGVPVCPHAGGVGLCELVQHLSIFDYVAVSADLADRVVEFVDHLHEHF
                     VDPVVVRDGRYLPPSRPGYSAEMHRGSLLRYRFPDGPAWSAAAPGSGAPGSVAPPATP
                     RPRIPEEGQPAATT"
     gene            complement(2806..>2994)
                     /locus_tag="VEP73_09665"
     CDS             complement(2806..>2994)
                     /locus_tag="VEP73_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT26734.1"
                     /translation="GVGHLIWYLSQFMVLEPGDLINTGTPAGVGLGMDPPGYLCAGDV
                     VELEVTGLGRQRQTLRDA"
BASE COUNT          399 a         1071 c         1163 g          361 t
ORIGIN      
        1 gtcgtttcct ggcggtcgtc ggtcgttcgc ggccggctca ggcgcggtcc gcgcttgcga
       61 gggtgcccag gtcgcgcgcg cccgggcggt cgggccgggg gcccgggcgc gcggccagca
      121 gcagcacggc gaccagggcg aaggcggaga gcgccatcag cgggatggag atgtagtgga
      181 acctccagac ccacaggagg gtacacgggt tgtccgggtc gcaggttccc gactcgagcg
      241 tcggaacgcg ctcgagaagg tagtggtagg cggagatcgg ggcgccgacc agggccagcg
      301 cgaggccgta ggggcggacc gcgaggtcgc gacgccaggc ggccacgccg aggacgagcg
      361 ccagggggta catcgccgcc cgctggtacc agcacagctt gcagggcgtg aagtgggcca
      421 cctcggacag gtagaggctg ccgagcatgg ccaccagcgc caccaggaag gcgagctgga
      481 gcgcctgcgg gcggatcgcg gcggcgagct cggcccgcag gcgggccgct gccggccacc
      541 acctgccggt cacggcgagg acgagcgcga cggcgagcgc gagctgggcg ccgaccgtca
      601 gcagggcgag gaacagcgtc accgccggcg tgctcacgcg aagggctcct ggtccgttgg
      661 gtgtcgccgg gggcgaccgc gggcccgccg ccgcccgtgc cgggtccgcg cccggcctgg
      721 tgctcccatc gtacgcggca ccgggccctg ctcgcggacg cggcgccggg ccttcccggt
      781 gccgggccgc cagttgcgat atagttgcaa ctacgcacag acgcgagtga cgtccggcgg
      841 acggagaggc ggcgacatgg acacgctgga agcgctcgag cgggggtggg acgaggacgc
      901 ggccacctac gacgccacca ccaaccacgg catggcgacg cccgcggagc gggccgcctg
      961 gtcggccgcg ctcgcccgcc agctcccacc cggcgcgtcc gtgctcgacg tcggcgcggg
     1021 caccggcttc ctgtcgctgg ccgcctggcg cgaggccgcc cccccggggc cgcctcgcgc
     1081 tcttcgagag catgtggggc cgcacggacc cggccgagat cgtccgccag cgcctgcggc
     1141 acggcgtcga ccggctgcgc cgggcgcacc acgggcacca cggcgagtac gacccgagcg
     1201 tgagcgccca gcttccgtac ggcgaggggc tcagcccgag cgcgctcgtc gagctggtgg
     1261 agggcagcgg ctgggccagg cccgcctcga gcggctccgt gacgtcgagt ggacccgcct
     1321 gctcgcccgc ccaccgctcg agcgggtcct cggcgtgacg ccgctctacg tcgtcgccgc
     1381 cggctgaccc tcctccggga tccggggccg cggggtcgcg ggcggcgcga ccgaccctgg
     1441 ggccccggat cccggagcgg cagccgacca ggccggcccg tcggggaagc ggtaccgcag
     1501 gagcgagccg cggtgcatct cggcggagta cccgggccgg gacggcggca ggtagcggcc
     1561 gtcacggacg acgaccgggt cgacgaagtg ctcgtgcagg tggtcgacga actcgacgac
     1621 ccggtcggcg aggtcggcgc tgaccgcgac gtagtcgaag atcgacaggt gctggaccag
     1681 ctcgcagagc ccgaccccgc cggcgtgggg gcagacgggc accccgaact tggcggccat
     1741 cagcaggacg gcgagcacct cgttgacccc gccgagccgg caggcgtcga cctggcagaa
     1801 gcggatcgcc cgcgcctgca ggagctgctt gaagaccacc cggttctggc agtgctcgcc
     1861 ggtggcgacc ccgatcgggg cgatcgccct ggcgatcgcg gcgtggccga gcacgtcgtc
     1921 ggggctcgtc ggctcctcga tccaccaggg gtcgaactcg gcgagccgcc ccatcgccgc
     1981 gatcgcctcc ccgacgtccc agatctggtt ggcgtccatc atcagcttgc ggtcgggccc
     2041 gatctcggcg cggacgatcc ccgcgcggcg cacgtcgtcg tcggtgtccc ggcccacctt
     2101 gatcttcacg tgggtccagc cctccccgac cgcctcccgg cagaggcgcc ggagcttgtc
     2161 gtcggggtac ccgagccagc cggccgaggt cgtgtaggcc gggtagcccc gctcgagcat
     2221 cgcctcggcg cgacggcccc gggccggctc ggccgcgcgc aggatctcga gcgcctcgcc
     2281 cggggtcagc gcgtcggtga tgtagcggaa gtcgacgcag ccgaccaggt cctcggggga
     2341 cagctcggcg agcaggcgcc acagcggctt gccctcgacc ttggcgtaca ggtcccacgt
     2401 ggcgttcacg acggcggcgg tcgcgaggtg gatcgcgccc ttctcagggc cgatccagcg
     2461 gagctggctg tcggaggtga cccggcgcca gaacgcgccc atgtcggcga agaagcggtc
     2521 ggcgtcctcg ccgaccacga gcggcgcgag cgcgcggatg gcggccgcca cgacctcgtt
     2581 gccccggccg atcgtgaacg tgaacccgtg gccctcgagg ccgtcgccgg cgctggtgtg
     2641 gaggatgcag tacgcggcgg agtagtcggg gtccgggttc atcgcgtccg agccgtcgag
     2701 cgaccgcgag gtcgggaagc ggacgtcgaa gacctccacg ccggtgatcg tccttgacat
     2761 ggcctgtgct cctgttcggg ttccaggcgc cgggcgcggt cgcgctcagg cgtcgcgcag
     2821 ggtctgccgc tggcggccga ggccggtgac ctcgagctcc acgacgtcgc cggcgcacag
     2881 gtagccgggg gggtccatgc ccaggccgac gccggccggg gtgccggtgt tgatcaggtc
     2941 gccgggctcg aggaccatga actggctcag gtaccagatg aggtggccca cgcc
//
LOCUS       DASPYY010000615         5485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2210742, whole genome shotgun sequence.
ACCESSION   DASPYY010000615 DASPYY010000000
VERSION     DASPYY010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210742"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VEP73_09670"
     CDS             <1..529
                     /locus_tag="VEP73_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYT26735.1"
                     /translation="IFAYGEVPRSLELAGRITRLATGVVASLAVNRERMREVLLSGFC
                     QATDLAEFVMQACGVDYRTAYLVVGRAVRQASRQGLRGIDLTGELLDAAAREQLGRPL
                     GLAGRDLAEVLDPRAIVLTRLAPGGAAPEVVRGMARSCTEAADRLLAVAAGHAERFRL
                     AEEALLNQAREVARG"
     gene            complement(498..905)
                     /locus_tag="VEP73_09675"
     CDS             complement(498..905)
                     /locus_tag="VEP73_09675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYT26736.1"
                     /translation="MTARWEGAYRCRVSVRHFELRVDEPPPRGGDTGFQPTELFLSSL
                     ASCFTLAVAHAAGKRGVELPDLSVRAVGDYDGLRFGKLRVEVSSSHPRAELTRLVERA
                     MPFCYVSNTLRGQPELEFVVAGPPSHEPPPAPG"
     gene            967..2349
                     /locus_tag="VEP73_09680"
     CDS             967..2349
                     /locus_tag="VEP73_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HYT26737.1"
                     /translation="MRVVNLGLPVFGDAVRAQGAAAVDVDWRIPAGGHPELVAALERL
                     CGPLSARVEAGNREVVRRLDEGAPALTGIAPAAEVVPGMGARTILHCGPPLDWEDFCD
                     PLRRSVRASVLAEGWASSPEEAERLVARGRVRLEPANHHAAVVPMATTLGPSAPVFVV
                     ENHQGGNRAYSGLNQGPGAAAWFGMDAPAAVERLVWLRDVAAPVLGATIAGAGPVELF
                     PLAAQGLQMGDDVHMRTQATTNLLLRHLLPQLVAVDSPLLVEVARFLSGNHLFFLNLA
                     MAAAKAVADWAGQVADSSVVVGMARNGTTFGIRLAGTGDAWFVAPAPPVGRALYHPGF
                     GPEASAPDIGDSAVLELVGLGGPAAAASPAVAAFLGGTMAAAAAATEAADHVCAGRSS
                     RFKLPPLDFRGAPIGVDVRKAVELEITPSINTGILHATAGTGQVGAGIAEAPIECFRR
                     ALLALDERLR"
     gene            complement(2404..2892)
                     /locus_tag="VEP73_09685"
     CDS             complement(2404..2892)
                     /locus_tag="VEP73_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HYT26738.1"
                     /translation="MPKTVDTPRAEEGAYLYNTEEKVFEDIQANPGEQAFVFLHTVPY
                     EGSVGLVNLLTATRLVRKGFDVHVVLYGPGVLMASATRGYPSVGKEGFPGNLAINNQI
                     KTLLKEGAHVYACRFAMGALYGFREDDLIEGVKAFNPLDVLDSALAAWRAGAFQLNTW
                     TV"
     gene            complement(2931..3218)
                     /locus_tag="VEP73_09690"
     CDS             complement(2931..3218)
                     /locus_tag="VEP73_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26739.1"
                     /translation="MSFSQWLRANAERYLLERAQADLARRYLDRPGPVQEAGLGPFFW
                     RRVFVPVYRRLPWGLRRSVIQVLPGSHRRRWQASRASGGVARRPVRRQPTA"
     gene            complement(3222..4556)
                     /locus_tag="VEP73_09695"
     CDS             complement(3222..4556)
                     /locus_tag="VEP73_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT26740.1"
                     /translation="MAPQIVIVGGGAAGIGAAGSARQVDPDARVTVYTEYEDAAYSPC
                     GIPYVHGREIADFESLFLQGKEAYERAGIDIHYETRVTALDLGRRRVTVEGEGEVPFD
                     RLVLATGFDYADPGVPGSDLDGLYYVKNIRRAMEWDRVLDGVRVAVVVEASPLGLEMV
                     TALAHRGIETHLVDPHPWLLAEAADPDIMRPVEESWAELGVKMHFNTRVAAFVGDRRV
                     RAVGTSEGELPAEVVVVCTHKQPNNTLARRAGVRIGSTGGIVVDERMATPVEGVFAAG
                     DCVELPHGVTNIPIQGLSGSHAYAQGKVAGSAAAGGTRTYQPVYVPWGMVAGKWMIGG
                     ASFGETTATALGIPFVMGVAEGISRARYYPGVKKLRVKLLASPGSLRLIGAQLVGQEG
                     IKERADFLAMAIKTGVTLEDLAWMENVYSPPIGALNEPIALAAQNGLASIRR"
     gene            4692..5357
                     /locus_tag="VEP73_09700"
     CDS             4692..5357
                     /locus_tag="VEP73_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT26741.1"
                     /translation="MSGDEAAAPPKPGRARGPARAAAKARTSASAGQADQLGQIIESI
                     GPKVRHLRRLKGLSLQQLGERSDISAAAIHKIERNGMVPTITTLMKLAAALNRSVAYF
                     VDEEQAEPAVLIRCDERKPVFTSKERLDLAGISGPYGHFFMAGAVATVEPGADSGPKH
                     MEHPGEELVFMLDGTMSFEVEGVEYMLRRGDALHFRTDRPHRWGNPAARPARAIWMAL
                     RST"
BASE COUNT          730 a         2014 c         1995 g          746 t
ORIGIN      
        1 gatcttcgcc tacggggagg tgccgcgctc cctggagctg gccggcagga tcacccgcct
       61 ggcgaccggc gtggtggcga gcctcgccgt gaaccgggag cggatgcggg aggtgctcct
      121 gtccggcttc tgccaggcca ccgacctggc cgagttcgtc atgcaggcgt gcggggtgga
      181 ctaccgcacg gcctacctgg tggtcggcag ggcggtccgc caggccagcc ggcaggggct
      241 gcgcgggatc gacctgaccg gcgagctgct cgacgcggcg gcgcgcgagc agctcggccg
      301 gccgctcggg ctcgccggcc gggacctcgc cgaggtcctc gacccgcggg cgatcgtgct
      361 gacccgactg gcccccggcg gcgccgcacc ggaggtcgtg cggggcatgg cccgctcctg
      421 caccgaggcc gctgaccggc tcctcgccgt ggccgccggg cacgccgagc gcttccgcct
      481 ggccgaggag gccctgctca accaggcgcg ggaggtggct cgtggctagg cgggcccgcg
      541 acgacgaact cgagctccgg ctgcccgcgc agggtgttgg agacgtagca gaacggcatg
      601 gcccgctcca ccagccgggt cagctccgcc cggggatggc tggagctgac ctcgacgcgg
      661 agcttgccga aacgcagccc gtcgtagtcg ccgacggccc gcaccgacag gtcgggcagc
      721 tcgaccccgc gcttgccggc ggcgtgggcg acggccaggg tgaagcacga cgccagcgac
      781 gacaggaaca gctcggtcgg ctggaagccg gtgtcgccgc cgcggggcgg cggctcgtcc
      841 acccgcagct cgaagtggcg caccgacacc cggcaccggt acgccccctc ccatcgggcc
      901 gtcacctcca tcaccgtgca cgctaacccg ccgccccggc gcgatccagt cccgctcccc
      961 ggcgaggtgc gggtcgtcaa cctcggcctg ccggtgttcg gcgacgcggt ccgcgcccag
     1021 ggggcggccg cggtcgacgt cgactggcgc atccccgccg gtggccaccc ggagctggtc
     1081 gccgcgctgg agcggctctg cgggcccctc tcggcccggg tggaggcagg caaccgcgag
     1141 gtggtccgcc ggctcgacga gggggcgccg gccctgaccg ggatcgcccc ggccgccgag
     1201 gtggtcccgg ggatgggcgc ccggaccatc ctgcactgcg gccctcccct cgactgggag
     1261 gacttctgcg atccgctgcg ccgctcggtc cgcgcctcgg tgctggccga gggatgggcg
     1321 tcctcgccgg aggaggccga gcggctggtg gccaggggac gcgtgcggct cgagccggcc
     1381 aaccatcacg ccgcggtcgt gccgatggcg accaccctcg gcccctcggc ccccgtgttc
     1441 gtggtcgaga accaccaggg cgggaaccgc gcctactccg ggctcaacca ggggccgggc
     1501 gcggcggcct ggttcgggat ggacgctccg gcggcggtcg agcggctggt ctggctgcgc
     1561 gacgtcgccg caccggtgct gggcgccacg atcgccgggg ccgggccggt cgagctgttc
     1621 ccgctggccg cccagggcct gcagatgggc gacgacgtcc acatgcggac gcaggcgacc
     1681 acgaacctgc tcctgcgcca cctgctcccg cagctcgtgg cggtcgactc cccgctcctc
     1741 gtcgaggtgg cccggttcct gtcaggcaac cacctgttct tcctgaacct ggccatggcc
     1801 gcggccaagg cggtcgccga ctgggccggc caggtcgcgg actcgagcgt ggtggtgggg
     1861 atggcccgca acggcaccac gttcggcatc cggctggctg gcacgggcga cgcctggttc
     1921 gtcgcccccg ccccgccggt cggccgggcg ctctaccacc cggggttcgg cccggaggcg
     1981 agcgcgcccg acatcggcga cagcgcggtc ctcgagctgg tcgggctggg cgggccggcg
     2041 gcggccgcgt ccccggcggt ggccgccttc ctgggcggga cgatggccgc cgcggcggcg
     2101 gccaccgagg ccgcggacca tgtctgcgcg ggccggagca gccgcttcaa gctcccgccg
     2161 ctcgacttcc gtggcgcccc gatcggcgtg gacgtgcgca aggcggtgga gctcgagatc
     2221 accccgtcga tcaacacggg catcctccac gccaccgcgg ggaccggcca ggtgggcgcc
     2281 gggatcgcgg aggcgccgat cgagtgcttc cgccgggcgc tgctcgccct cgacgagcgt
     2341 ctccgatgag gtgggcggcg cggaagtcgg cgcctcgtcg ggcgccgact tccgccgccc
     2401 cgctcagacc gtccaggtgt tgagctggaa ggctccggcc cgccaggcgg ccagcgccga
     2461 gtccagcacg tcgagcgggt tgaacgcctt caccccttcg atcaggtcgt cctcccggaa
     2521 cccgtacagg gctcccatgg cgaagcggca ggcgtagacg tgggcgccct ccttgaggag
     2581 ggtcttgatc tggttgttga tggccaggtt gccggggaag ccttccttgc ccaccgacgg
     2641 gtagccgcgg gtggccgacg ccatcaggac acccggtccg tagaggacga cgtggacgtc
     2701 gaaccccttg cggaccaggc gggtggcggt gaggaggttc accaggccga ccgagccctc
     2761 gtagggaacg gtgtgcagga acacgaaggc ctgctcgccc gggttcgcct ggatgtcctc
     2821 gaacaccttc tcctcggtgt tgtagaggta ggccccctcc tcggcccgtg gcgtgtcgac
     2881 ggtctttggc atggtgtccg ctcctcctgc tcggggtcgg gatgctctgg ctaggcggtg
     2941 ggctggcggc ggaccggccg ccgggccacc ccgcctgaag cgcgcgaggc ctgccagcgg
     3001 cgacgatggc tgccggggag tacctggatg acggaccgcc gcagccccca tgggaggcgc
     3061 cggtagacgg gtacgaacac gcgccgccag aagaaggggc ccagacccgc ttcctgcacc
     3121 ggccccgggc ggtccaggta ccgccgggcc aggtcggcct gcgcgcgctc gagcaggtag
     3181 cgctcggcgt tggcgcgcag ccactgcgag aagctcaccc atcacctcct gattgacgcc
     3241 aggccgttct gggcggccag ggcgatcggc tcgttcagcg ccccgatggg cggcgagtag
     3301 acgttctcca tccaggccag gtcctcgagg gtcaccccgg tcttgatggc catggccagg
     3361 aagtcggccc gctccttgat cccctcctgg ccgaccagct gggcaccgat cagccgcagg
     3421 ctgcccggcg aggccagcag cttcaccctg agcttcttca cccccgggta gtagcgggcc
     3481 cgggagatcc cctcggccac gcccatgacg aacgggatgc cgagcgcggt cgcggtcgtc
     3541 tccccgaacg aggcgccgcc gatcatccac ttgccggcca ccatccccca gggcacgtag
     3601 acgggctggt aggtccgggt cccgccggcg gcggcggacc cggccacctt gccctgggcg
     3661 tacgcgtggc tgcctgacag cccctggatc gggatgttgg tgaccccgtg gggcagctcc
     3721 acgcagtcgc cggccgcgaa gacgccctcc acgggcgtgg ccatgcgctc gtcgaccacg
     3781 atcccgccgg tggagccgat ccggacgccg gcccggcggg cgagggtgtt gttgggctgc
     3841 ttgtgggtgc acaccaccac gacctcggcc ggcagctcgc cctcggaggt ccccaccgcg
     3901 cgcaccctcc ggtcgcccac gaacgccgcc acgcgggtgt tgaagtgcat cttcacgccg
     3961 agctcggccc acgactcctc gaccggccgc atgatgtcgg ggtcggccgc ctcggccagc
     4021 agccacggat gggggtcgac caggtgcgtc tcgatgccac ggtgggccag ggcggtgacc
     4081 atctcgaggc cgagcggcga cgcctcgacc acgaccgcga cccgcacgcc gtcgaggacc
     4141 cggtcccatt ccatggcccg gcggatgttc ttgacgtagt acaggccatc gaggtcggac
     4201 cctgggacgc ccgggtcggc gtagtcgaag ccggtggcga gcaccaggcg gtcgaagggc
     4261 acctcgccct cgccctccac ggtgacccgt cgccgcccga ggtcgagcgc ggtcacccgg
     4321 gtctcgtagt ggatgtcgat gccggcccgc tcgtaggcct ccttgccctg caggaacagg
     4381 gactcgaagt ccgcgatctc ccggccgtgg acgtagggga tgccacaggg gctgtaggcg
     4441 gcgtcctcgt actcggtgta gacggtcacc cgcgcatccg gatcgacctg cctggcgctg
     4501 ccggcggccc cgatgcccgc ggcgccaccc cccacgatga cgatctgcgg cgccatcctc
     4561 gcccccagga tcaccatttc ggaagctggt gcgacagcga attcaccatg ataaatgcag
     4621 catcgcctat actactcggt gcgtccagac cgtcaaggag agacgccttg ggtggagaag
     4681 aaggcgtgcg ggtgagcggc gacgaagccg ccgccccgcc gaagccgggc cgggcccgtg
     4741 gacccgcgcg ggccgccgcc aaggcgcgca cgtcggcgtc ggctgggcag gccgaccagc
     4801 tcggccagat catcgagtcg atcgggccga aggtccgcca cctccggcgg ctcaagggcc
     4861 tgtcgctcca gcagctcggc gagcggtcgg acatctccgc cgcggccatc cacaagatcg
     4921 agcgcaacgg gatggtcccg acgatcacga ccctgatgaa gctcgccgcc gccctcaacc
     4981 ggtccgtcgc ctacttcgtc gacgaggaac aggccgagcc ggccgtcctc atccgctgcg
     5041 acgagcgcaa gccggtcttc acctccaagg agcgcctcga cctggccggc atcagcggcc
     5101 cgtacgggca cttcttcatg gccggagccg tcgccaccgt cgagccgggg gcggactccg
     5161 ggccgaagca catggagcat ccgggtgagg agctggtctt catgctcgac ggcacgatga
     5221 gcttcgaggt cgagggtgtt gagtacatgc tgcgccgcgg cgacgccctg cacttccgca
     5281 ccgaccgacc tcaccgctgg gggaacccgg cggcccggcc cgcccgcgcc atctggatgg
     5341 cgctgcggtc gacctgagaa gaaagcaagc accggcgttc atggctcgtc ctcgcatcgc
     5401 cgtggtggcc gtcggcggca atgcgctcac gcgcgagggt gaggcgggga cctacaccca
     5461 gcagtggcgc aatgccaccc gggtg
//
LOCUS       DASPYY010000616         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2772916, whole genome shotgun sequence.
ACCESSION   DASPYY010000616 DASPYY010000000
VERSION     DASPYY010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772916"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VEP73_09705"
     CDS             complement(<1..434)
                     /locus_tag="VEP73_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYT26742.1"
                     /translation="MSKPVDLDPVDQRIVAALQQDGRRPFTRIAKELGISEAAVRQRV
                     ARLQALGAMQVVAVADPMRLGYKTMAMVAISVDGRSLARVAEAVRRLPEVSYLVLTAG
                     SHDMLAEVVCEDNDHLLRLLSEDLAGIEGIRETETFMYLRLL"
     gene            530..2059
                     /locus_tag="VEP73_09710"
     CDS             530..2059
                     /locus_tag="VEP73_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYT26743.1"
                     /translation="MQGERPCAAPRSVITMGQRPAQERIEALTTARTQLVHERTPTSR
                     AWRERSLRSLPLGVPSSFQDSPPYPIFFERGQGSHVWDVDGNEYADYHNGFGVMCVGH
                     AHPKIVGAISERAATGTHFAQPVAETTLLAEEICQRFQLDQMRFTNSGTEATMDAIRL
                     ARGATGRDKIAKVEGSYHGHHESVLVSIRPSRDDMGPRERPASVAFGKGTPAGVTDLT
                     LVVPYNDAEALDRLLAAHAGEVAALIMEPVMMNVGIIDPLPGYLEAVRDVCTRHGVLL
                     IWDEVKTGATIAHGGAEEVFGVAPDLKCFAKAVGGGVPIGAFGGRGELMEEIAKDGVP
                     QLGTFNGNPLSARVGLVALTEVLTHDAYKELNRLNDAQLAGCQRVIDGYGLPVYTTGM
                     GAKGAVMYAPSPLKDYREYAGFDAPGVDADLSYLSWLYEVTEGVFMTPGIDEQWTLSV
                     QHTDADVARYVRAFETFASEVTGRVEVDLPEAPAGAGQGLPDGPARPGEGGARLSGLP
                     R"
     gene            2083..>2468
                     /locus_tag="VEP73_09715"
     CDS             2083..>2468
                     /locus_tag="VEP73_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYT26744.1"
                     /translation="MRHPGQDHSARLDALLAPGAVAVVGAAPGNAAAVTVVRNLVDLG
                     YPGAVYPVHPAAAPVCGLPAYPSLADLPGPVEAAALLVGARRVPAAADAAVAAGAAAL
                     WVFAGGRADGEAGRAMEAHLAGLAART"
BASE COUNT          342 a          935 c          872 g          319 t
ORIGIN      
        1 ttgagcaggc gcaggtacat gaacgtctcg gtctcgcgga tgccctcgat cccggccagg
       61 tcctccgaca agaggcgcag gaggtggtcg ttgtcctcgc agaccacctc ggcgagcatg
      121 tcgtggctgc ccgcggtgag caccaggtag gagacctctg gcagccgccg cacggcctcg
      181 gcgacccggg cgagcgagcg gccgtcgacc gagatcgcca ccatcgccat cgtcttgtag
      241 ccgagccgca tcgggtcggc gacggcgacg acctgcatcg ccccgagcgc ctgcaggcgg
      301 gcgacccgct ggcgtacggc cgcctccgag atgccgagct ccttcgcgat cctggtgaac
      361 ggccggcgcc cgtcctgctg cagcgccgcc acgatccgct ggtccaccgg gtcgagatcg
      421 accggcttgc tcattcgaac caccctccac aaccgggttg actgcttcgt agtgtgcacc
      481 atttcgttga ccagagcacg aatctcgaag tatggtgacg gccgaaccgg tgcagggcga
      541 acgcccctgc gccgctccta ggagcgtgat caccatgggc cagcggcccg cccaggagcg
      601 catcgaggcg ctcaccaccg cccgcaccca gctcgtgcac gagcgcacgc ccacctcgcg
      661 tgcatggcgc gagcggtcgc tcaggtcgct gccgctcggg gtgccgtcca gcttccagga
      721 ctccccgccc tacccgatct tcttcgagcg cggccagggg agccacgtct gggacgtcga
      781 cggcaacgag tacgccgact accacaacgg cttcggggtg atgtgcgtcg gccacgccca
      841 cccgaagatc gtcggggcga tctccgagcg ggccgcgacc ggcacccact tcgcccagcc
      901 ggtcgccgag acgaccctcc tcgccgagga gatctgccag cgcttccagc tcgaccagat
      961 gcgcttcacg aactcgggta ccgaggcgac catggacgcc atccggctcg cgcgcggcgc
     1021 gaccggccgc gacaagatcg cgaaggtcga ggggtcctac cacggccacc acgagtcggt
     1081 cctggtctcg atccgcccca gccgggacga catggggccg cgggagcgcc cggccagcgt
     1141 cgcgttcggc aagggcaccc cggccggcgt caccgacctc accctggtcg tgccctacaa
     1201 cgacgccgag gcgctcgacc ggctgctcgc cgcgcacgct ggcgaggtgg ccgcgctcat
     1261 catggagccg gtcatgatga acgtcgggat catcgacccg ctgcccggct acctcgaggc
     1321 cgtccgcgac gtctgcaccc gccatggcgt cctgctcatc tgggacgagg tgaagacggg
     1381 cgcgacgatc gcccacggcg gcgccgagga ggtcttcggc gtcgccccgg acctcaagtg
     1441 cttcgccaag gcggtcggcg gcggcgtgcc gatcggggcc ttcggcggcc ggggcgagct
     1501 gatggaggag atcgcgaagg acggcgtgcc gcagctcggc accttcaacg gcaacccgct
     1561 gtcggccagg gtcggcctgg tcgccctcac cgaggtcctc acccacgacg cctacaagga
     1621 gctgaaccgg ctgaacgacg cccagctcgc gggctgccag cgggtcatcg acggctacgg
     1681 cctgccggtg tacacgaccg gcatgggtgc caagggcgcg gtcatgtacg cgccgagccc
     1741 gctcaaggac taccgggagt acgccggctt cgacgcgcca ggggtggacg ccgacctgtc
     1801 gtacctgtcg tggctgtacg aggtgaccga gggcgtgttc atgaccccgg gcatcgacga
     1861 gcagtggacc ctctcggtgc agcacaccga cgccgacgtc gcacgctacg tccgcgcctt
     1921 cgagacgttc gcgagcgagg tgaccggccg cgtcgaggtc gacctgcccg aggcgcccgc
     1981 aggggccggc cagggccttc ccgacggccc cgcgcggccc ggcgagggcg gggcgcgcct
     2041 cagcggcctg ccgcggtagg ccggcagcgg cccgcgcgcg gcgtgcggca cccggggcag
     2101 gaccactcgg cgcggctcga cgcgctcctc gcgccgggag cggtcgccgt ggtcggcgcg
     2161 gctcccggca acgccgccgc cgtgaccgtc gtccgcaacc tggtcgacct cggctacccg
     2221 ggcgccgtgt acccggtgca cccggccgcg gcgcccgtct gcggcctgcc tgcctacccg
     2281 agcctcgccg acctgcccgg gccggtcgag gcggccgcgc tgctcgtcgg ggccaggcgc
     2341 gtgcccgccg cggccgacgc cgccgtcgcg gccggcgcgg ccgccctgtg ggtgttcgcc
     2401 ggcgggcggg ccgacggcga ggccggccgg gcgatggagg cccacctcgc cggcctcgcc
     2461 gcacggac
//
LOCUS       DASPYY010000617         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1649392, whole genome shotgun sequence.
ACCESSION   DASPYY010000617 DASPYY010000000
VERSION     DASPYY010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649392"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VEP73_09720"
     CDS             complement(<1..473)
                     /locus_tag="VEP73_09720"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYT26745.1"
                     /translation="MLRVLRALALGAHPDDVELQAGGTIAVWARGGVHVEIGCFTAGG
                     KGTADPAADPAEVAAVRRRETEVAAGVLGVAAVHFLGAVDGELEATMALRLAVARLVR
                     AVRPDVVLGHDPWRRWLLHPDHRAAGLLTVDGVVAARDPLYAPGLAPAHRPHTVLL"
     gene            complement(562..1929)
                     /gene="mshA"
                     /locus_tag="VEP73_09725"
     CDS             complement(562..1929)
                     /gene="mshA"
                     /locus_tag="VEP73_09725"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018221555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HYT26746.1"
                     /translation="MDSSSAPGASSRVRGAKERARGFRVPQTNGQASASGGSEVRRVA
                     VLSVHSSPLAPPGVGDSGGMNVYVRALARELAARGVESDLYTRAASPDDPPVVEVEPG
                     VRLLHLEAGPLHPVPKQTLPRHLCAFLCSLLRAGERYGPYDVLHSHYWLSGWVARRAR
                     ERWGAPVVHSFHTLGRVKNATLVDGDQPEPPVRVAGEERVVATADRLVAPTPAEARQL
                     VELYAASPAAVRIVPPGVDHARFRPGDRAAARAALGTRARFVIAFVGRIQPLKGADVA
                     VRALAALAARQDALDVELLVVGGVSGNGGGEPARLERLAREAGVGGRVRFLPPQPHER
                     LAEVFRAADVVLVPSYSESFGLVALEAQACGTPVVAAQVGGLVHAIGDGTTGLLVPGH
                     DPAAFASAVAGLLASPRRLAAMGAAAARFAGAHGWDRTAARMLDIYGELTVPAAAEPV
                     LEQTS"
     gene            complement(1994..2413)
                     /locus_tag="VEP73_09730"
     CDS             complement(1994..2413)
                     /locus_tag="VEP73_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26747.1"
                     /translation="MRKGIEQLDPAGAVLGVGRYIREQRRQAELSLRNLAKQAGVSNP
                     YLSQIERGLRQPSAKILKDIAKALRISAETLYVKAGILDEDAEDADLVVAILRDPHLT
                     ERQKQVLVDVYTSFRAGGQAPAAAPKPEPAGEPPATA"
     gene            complement(2503..>2938)
                     /locus_tag="VEP73_09735"
     CDS             complement(2503..>2938)
                     /locus_tag="VEP73_09735"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYT26748.1"
                     /translation="VHAVPLRGLATAYARLGTRAATGQEGPAALVAAVRGHPFMVSGT
                     GGLDTVVLEATGGRVFSKVGAEAAYGAVRLDTGEGLALKVADGSARARGAALLALLRA
                     LGWLDEAELEAVMPAATSEVRGGGEPVGMVRPATVELEALDG"
BASE COUNT          353 a         1120 c         1159 g          306 t
ORIGIN      
        1 agcaggaccg tgtggggccg gtgcgcgggc gccagcccgg gcgcgtagag ggggtcgcgc
       61 gcggccacca cgccgtccac ggtgagcagc ccggccgccc ggtggtcggg gtggaggagc
      121 cagcgccgcc aggggtcgtg cccgaggacg acgtcgggcc ggaccgcgcg caccagccgg
      181 gccacggcca ggcgcagcgc catggtcgcc tccagctcgc cgtcgaccgc gccgaggaag
      241 tgcaccgcgg ccacgccgag cacgcccgcg gccacctcgg tctcccggcg ccgcaccgca
      301 gccacctccg ccgggtcggc cgccgggtcg gcggtgccct tcccgcccgc ggtgaagcag
      361 ccgatctcga cgtggacgcc gcccctggcc cacaccgcga tcgtgccgcc ggcctggagc
      421 tcgacgtcgt cggggtgcgc gccgagcgcg agcgcccgca gcaccctcag cacgccgccg
      481 tcctcccctc ccagtcgtcc gctgccccgc ccccggtcgt ccaccgctcc cggcgcgagg
      541 caccgcccgc cggaccggtg gtcagctcgt ctgctcgagg accggctcgg cagccgccgg
      601 gacggtgagc tcgccgtaga tgtcgagcat gcgggccgcg gtccggtccc agccgtgggc
      661 gcccgcgaac cgggcggccg cggcgcccat ggcggcgagc cggcgcgggc tggcgagcag
      721 cccggccacc gccgaggcga acgcggccgg gtcgtggccc gggacgagca ggccggtcgt
      781 cccgtccccg atggcgtgga cgaggccgcc gacctgggcg gcgaccaccg gcgtgccgca
      841 cgcctgggct tcgagggcga ccagcccgaa cgactcggag taggacggga cgagcaccac
      901 gtcggcggcc cggaacacct cggccaggcg ctcgtggggc tgcgggggca ggaagcgcac
      961 gcgcccgccg accccggcct cgcgggccag gcgctcgaga cgggccggct ccccgccgcc
     1021 gttgccggag acgccgccga ccacgagcag ctcgacgtcg agcgcgtcct gccgcgcggc
     1081 cagggcggca agggcgcgca cggcgacgtc ggcgcccttg aggggctgga tgcggccgac
     1141 gaaggcgatc acgaagcggg cgcgggtgcc gagcgcggcg cgggccgcgg cccggtcgcc
     1201 gggccggaag cgggcatggt cgaccccggg aggcacgatg cgcacggcgg ccggcgaggc
     1261 cgcgtacagc tcgacgagct gcctggcctc ggccggggtg ggggcgacga ggcggtcggc
     1321 ggtcgcgacc acgcgctcct cgccggcgac ccggacgggc ggctcgggct ggtccccgtc
     1381 caccagggtg gcgttcttca cccggccgag ggtgtggaac gagtggacga ccggcgcccc
     1441 ccagcgctcg cgggcgcgcc gggccaccca gccggacagc cagtagtggc tgtggaggac
     1501 gtcgtagggg ccgtagcgct cgccggcccg gagcagcgag cagaggaagg cgcacaggtg
     1561 gcggggcagc gtctgcttgg gcaccgggtg cagcgggccc gcctcgaggt ggagcagccg
     1621 gacacccggc tcgacctcga ccacgggcgg gtcgtccggg gaggcggcgc gggtgtagag
     1681 gtccgactcc acgccacggg cggcgagctc gcgggcgagc gcgcggacgt agacgttcat
     1741 gccgcccgag tcccccaccc cgggaggggc gagcggcgag gagtgcaccg agagcacggc
     1801 gacccggcgc acctccgagc cgcccgacgc tgacgcctgg ccgttcgtct gcggcacccg
     1861 gaaccccctg gctcgctcct tcgccccgcg gacccgtgac gaggcgcccg gggcggatga
     1921 gctatccacc gggatcgcca tgctatcggc ccaggcgcgg acgggccacg cgcccgccgg
     1981 cacgggcggg gcgtcaggcc gtggcggggg gctccccggc cggctcgggc ttcggcgcgg
     2041 cggccggggc ctggccgccg gcccggaacg aggtgtagac gtcgacgagc acctgcttct
     2101 ggcgctcggt caggtggggg tcgcgcagga tggcgacgac caggtcggca tcttcggcgt
     2161 cctcgtcgag gatgcccgcc ttgacgtaga gcgtctcggc gctgatccgc agcgccttgg
     2221 cgatgtcctt gaggatcttg gccgacggct ggcgcagccc gcgctcgatc tgggacaggt
     2281 acgggttcga caccccggcc tgcttggcca ggttgcgcag ggacagctca gcctggcgcc
     2341 gctgctcgcg gatgtagcgg ccgacgccga ggacggcacc ggccgggtcg agctgctcga
     2401 tgcccttgcg caccgtgggg accactccct tccgtcgggc cgccacggta cgtccggtga
     2461 ttaacttttg caagcggagg agcggcctgg ccggccggca cctcagccgt ccagggcctc
     2521 gagctcgacc gttgccgggc ggaccatgcc gaccggctcg ccgccaccgc ggacctcgct
     2581 ggtcgccgcg ggcatgacgg cctcgagctc ggcctcgtcg agccagccga gcgcccgcag
     2641 cagcgcgagc agcgcggcgc ccctggcccg cgcggacccg tcggccacct tgagcgcgag
     2701 cccctccccg gtgtcgagac ggaccgcacc gtaggccgcc tcggcgccga ccttgctgaa
     2761 gacgcggccc ccggtcgcct cgagcaccac ggtgtcgagc ccgccggtgc cagagaccat
     2821 gaacgggtgc ccgcggacgg ccgcgacgag cgcggccggg ccctcctggc cggtcgccgc
     2881 cctggtgccg agccgcgcgt aggcggtggc caggccgcgc agcgggacgg cgtggacg
//
LOCUS       DASPYY010000618         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_384048, whole genome shotgun sequence.
ACCESSION   DASPYY010000618 DASPYY010000000
VERSION     DASPYY010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384048"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VEP73_09740"
     CDS             <1..590
                     /locus_tag="VEP73_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYT26749.1"
                     /translation="YKADRVPVGEDQRQHLELTREVAQTFNTRFGPTFPLPEPYIPKV
                     GGRVMDLQQPDAKMSKSAASPAGRVHLLDPPDTIRKKVRSAVTDSGREVAARPDKPAI
                     TNLLELYSIVSGRSVGELEQQYAGRGYGDFKGDLAEALVAWLAPVRERYEALRADPGH
                     LAGVLEQGADKARAIARETLAEVRDRMGFLPGRTG"
     gene            596..1561
                     /locus_tag="VEP73_09745"
     CDS             596..1561
                     /locus_tag="VEP73_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HYT26750.1"
                     /translation="MPKHRLAVAALLPEPVASHVQAWRRALREPTRDVVPPHVTIVPP
                     QSVRAEELEAAVALVERAAAEAVPAVVTLDGAGTFLPESPVVFLAVGEGAPALAAIEA
                     SLRRPPLDRRTHRFQPHVTIAQELPRPDLEQAVRDLAGFRASFPLREIALMEEDRGGV
                     WRPLRRMTAGASPLVREVPFTEAASAAVFLLDPPRVLLGLRTPDEGHRYPGAWDAIGG
                     KPDPGEPLLSALARETLEEAGVEPLDVTALGCFDDGERADAFYTATAWRGEPRNEAPS
                     EHTRLEWVPIHEAFGRSMPPTVRRALARLVEVVGASGTVPGSGPS"
     gene            1704..2177
                     /locus_tag="VEP73_09750"
     CDS             1704..2177
                     /locus_tag="VEP73_09750"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYT26751.1"
                     /translation="MAIQDTPSPDAGPARPGSGSAPPDEPSTAQLLKGLADDATTLVR
                     QEILLARQEMTEGLVNTAKASSFLVVAGVLAFYGLGFMLYTIAVAIGGPDWLGFLLVT
                     IVLFLVVGILGLLGRRRLSASKLAPEKARAELRETAAELREEIKWARRQPQQPGK"
     gene            2141..2377
                     /locus_tag="VEP73_09755"
     CDS             2141..2377
                     /locus_tag="VEP73_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26752.1"
                     /translation="MGETSAATAREVEETRRQMEEKVAKLSERAPEEARKLAKRIAFA
                     VLTAIAVFAARKLVDMLWERATGELPPTKVERDD"
BASE COUNT          323 a          902 c          855 g          302 t
ORIGIN      
        1 tctacaaggc cgaccgcgtg ccggtgggtg aggaccagcg ccagcatctc gagctcaccc
       61 gggaggtcgc ccagaccttc aacacccgct tcgggccgac cttcccgctc ccggagccct
      121 acatccccaa ggtcggcggc cgggtgatgg acctgcagca gcccgacgcg aagatgtcga
      181 agtcggccgc ctccccggcc ggccgcgtcc acctgctcga cccgcccgac acgatccgca
      241 agaaggtgcg ctcggccgtc accgactccg gccgcgaggt ggcggcccgc cccgacaagc
      301 cagcgatcac gaacctgctc gagctgtact cgatcgtgtc cggccgctcg gtcggcgagc
      361 tcgagcagca gtacgccggc cgcggctacg gggacttcaa gggcgacctg gccgaggcac
      421 tggtcgcctg gctcgcgccg gtccgcgagc gctacgaggc gctgcgcgcc gacccggggc
      481 acctcgccgg cgtcctcgag cagggcgccg acaaggcccg cgccatcgcc cgcgagacgc
      541 tcgcggaggt cagggaccgc atggggttcc ttcccggccg gaccggctga cgcccgtgcc
      601 gaagcaccgg ctcgccgtcg ccgccctgct gcccgagccc gtggcgagcc acgtccaggc
      661 ctggcggcgg gcgctgcgcg agccgaccag ggacgtcgtc ccgccgcacg tcaccatcgt
      721 cccgccccag tcggtgcgcg ccgaggagct cgaggcggcg gtcgcgctgg tggagcgggc
      781 ggccgccgag gccgtgcccg cggtggtgac gctggacggc gcgggcacgt tcctgcccga
      841 gagccccgtc gtcttcctcg ccgtcggcga gggcgcaccc gcgctcgcgg ccatcgaggc
      901 cagcctgcgc cggccgccgc tcgaccggcg gacgcaccgg ttccagcccc acgtcaccat
      961 cgcccaggag ctgccccggc ccgacctgga gcaggccgtc cgcgacctgg cggggttccg
     1021 cgcctcgttc ccgctgcgcg agatcgccct catggaagag gaccgcggtg gcgtctggcg
     1081 ccccttgcgc cgcatgaccg ccggcgcctc cccgctcgtc cgcgaggtcc cgttcaccga
     1141 ggcggccagc gccgcggtgt tcctcctcga cccgccgcgc gtgctgctcg ggctccgcac
     1201 cccggacgag ggccaccgct acccgggggc ctgggacgcc atcggcggca agccggaccc
     1261 cggcgagccg ctgctctcgg cgctcgcccg cgagacgctc gaggaggccg gggtggagcc
     1321 gctggacgtc accgccctcg gctgcttcga cgacggcgag cgggcggacg cgttctacac
     1381 cgccaccgcc tggcgcgggg agccgcgcaa cgaggcgccc tcggagcaca cgaggctcga
     1441 gtgggtgccg atccacgagg cgttcgggcg cagcatgccc ccgaccgtcc ggcgggcgct
     1501 ggccaggctc gtcgaggtcg tgggcgccag cgggacggtc cccgggtccg ggccgtcctg
     1561 accgcagcgc gcccgatctg ctccgggcac gggtaacaag gcaggtcacg gggtagttga
     1621 tggcaagatg tggcggacac tcgtctgccc ggtcccgcac gtcgatcccg cacgtcgacc
     1681 cgctagccgc tggagggagt acgttggcca tccaggacac gccgtcgcct gacgccggcc
     1741 cggcccggcc cggctccggc tccgctccac ccgacgagcc gtccaccgcc cagcttctca
     1801 agggcctcgc cgacgacgcg acgaccctcg tccgccagga gatcctcctg gccaggcagg
     1861 agatgaccga ggggctcgtg aacaccgcca aggcgtccag cttcctggtc gtcgccggtg
     1921 tgctcgcgtt ctacgggctc ggcttcatgc tctacacgat cgcggtggcg atcggcggcc
     1981 ccgactggct tggtttcctt cttgtcacta tcgtgctgtt cctggtggtc gggatcctcg
     2041 gcctcctggg gaggaggcgc ctctcggcct ccaagctggc gcccgagaag gcgagggcgg
     2101 agctgaggga gacggccgcg gagctgaggg aggagatcaa gtgggcgaga cgtcagccgc
     2161 aacagccagg gaagtagaag agacacgcag gcagatggag gagaaggtgg ccaagctgtc
     2221 ggagcgcgca cccgaggagg cacggaagct ggcgaagcgc atcgccttcg cggtgctgac
     2281 cgccatcgcc gtcttcgccg cgcgcaagct ggtcgacatg ctctgggagc gcgccaccgg
     2341 cgagctgccc ccgaccaagg tcgagcggga cgactgagcg cg
//
LOCUS       DASPYY010000619         1874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2211357, whole genome shotgun sequence.
ACCESSION   DASPYY010000619 DASPYY010000000
VERSION     DASPYY010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211357"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEP73_09760"
     CDS             <1..244
                     /locus_tag="VEP73_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26753.1"
                     /translation="RGGRAGRLAPGSACDLVVLDGRSYLDLAYHLGVNLARMVVKEGT
                     VIARGGVDTGIFTAPGGAGDVPDLEPDVEGNPPHGH"
     gene            234..1190
                     /gene="ftcD"
                     /locus_tag="VEP73_09765"
     CDS             234..1190
                     /gene="ftcD"
                     /locus_tag="VEP73_09765"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HYT26754.1"
                     /translation="MDTDRLVESVPNFSEGRRPEVVGEIVDAFAGAHPDVLVLDRSSD
                     ADHNRSVLTLAGPGEALVEAAVAGAVACARLIDLTVHTGAHPRIGALDVLPFVPLGET
                     TTADCVALAEQAGARLAGEVGVPVYYYGAAARRPERASLPKVRGKGFEELLATAATDP
                     ARAPDVGGPAPHPTAGATAVGVRPFLIAYNVDLATDDLDLARRIATAVRERDGGLPAV
                     RAMGFALPERGMVQVSMNLVDFRSTPPAAAFAAVAELAGQAGVRIADSEIVGLVPEAA
                     LQGTDPAALQLLAFAPDQFVEERVRRALAGREARRGAVGGAR"
     gene            1187..1804
                     /locus_tag="VEP73_09770"
     CDS             1187..1804
                     /locus_tag="VEP73_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HYT26755.1"
                     /translation="MSGPRLGEHPWLEPYAAPTAVPGGGSAAAVAGALGCALLAMAAR
                     VGARKADGERAETLGRLAASADALRAELLVREAADADAYERMVATARAARATTGDERA
                     ASRATRAVVEAALVPLGTAELAARGLGLAAELAACGLARTASDQLTAVHLLLAALEGG
                     LATVAVNLPGLPDAERDRLSQRAAELAEARRAGREAAALLGPLLA"
BASE COUNT          194 a          734 c          739 g          207 t
ORIGIN      
        1 gcggggcggc cgggcgggac ggctcgcgcc cgggtcggcc tgcgacctcg tcgtgctcga
       61 cgggcgctcc tacctggacc tcgcctacca cctgggcgtg aacctggccc ggatggtggt
      121 caaggagggg acggtgatcg cccgcggagg tgtcgacacc ggcatcttca cggcgccagg
      181 gggcgccggg gacgtaccag acctggaacc ggacgtggag gggaacccgc cgcatggaca
      241 ctgaccggct cgtcgagagc gtcccgaact tctccgaggg tcgccgcccc gaagtggtcg
      301 gcgagatcgt cgacgcgttc gccggggccc acccggacgt gctcgtgctc gaccgctcgt
      361 cggacgccga ccacaaccgc tcggtgctga ccctggccgg gccgggggag gccctggtcg
      421 aggcggcggt ggccggcgcg gtcgcgtgcg cccggctgat cgacctgacc gtccacaccg
      481 gcgcccaccc gcgcataggc gccctcgacg tgctgccgtt cgtgcccctg ggggagacca
      541 cgaccgccga ctgcgtggcc ctggccgagc aggcgggcgc tcggctggcc ggcgaggtgg
      601 gcgtgccggt ctactactac ggcgccgcgg cccgccggcc cgagcgggcg agcctcccga
      661 aggtccgcgg caagggcttc gaggagctgc tcgcgaccgc ggcgaccgac ccggcgcggg
      721 cacccgacgt cggcggcccg gcgccccacc cgacggccgg cgcgaccgcg gtgggggtgc
      781 ggcccttcct gatcgcctac aacgtcgacc tcgccaccga cgacctcgac ctggccaggc
      841 gcatcgccac cgccgtccgg gagcgcgacg gcggcctgcc cgccgtgcgg gcgatggggt
      901 tcgccctccc cgagcgcggc atggtgcagg tgtcgatgaa cctggtcgac ttccgctcga
      961 ccccgccggc ggcggcgttc gcggccgtcg ccgagctcgc cgggcaggcc ggtgtgcgga
     1021 tcgccgactc ggagatcgtg ggcctggtcc ccgaggccgc gctccagggc accgaccccg
     1081 cagccctcca gctgctcgcc ttcgcgccgg accagttcgt cgaggagcgg gtcaggcggg
     1141 cgctggccgg gcgggaggcg cggcgcggcg cggtcggggg tgcgcggtga gcgggccccg
     1201 cctcggcgag cacccgtggc tcgagcccta cgccgccccg accgcggtcc ccggcggcgg
     1261 cagcgccgcc gcggtcgcgg gggcgctcgg ctgcgccctg ctcgcgatgg cggcccgggt
     1321 cggggcccgc aaggcggacg gggagcgggc cgagaccctc ggccgcctcg ccgcctcggc
     1381 cgacgccctg cgcgccgagc tcctggtcag ggaggcggcg gacgcggacg cctacgagcg
     1441 gatggtcgcc accgcccgcg cggcccgggc gaccaccggc gacgagcgcg cggccagcag
     1501 ggccacgcgg gccgtcgtgg aggccgccct ggtcccgctc ggcacggccg agctggccgc
     1561 ccggggcctc ggcctggccg ccgagctcgc cgcgtgcggt ttggccagga ccgcgagcga
     1621 ccagctcacc gccgtccacc tcctgctcgc ggcgctcgag ggcggcctcg cgaccgtggc
     1681 ggtcaacctt cccgggctgc ccgacgccga gcgcgaccgg ctgtcccagc gggcggcgga
     1741 gctcgccgag gcccgccgcg ccggccgcga ggctgccgcg ctcctcgggc cgctgctcgc
     1801 atgagccggc ccgcagggga gccggtggtc ccgactccga gggggcgccg ctgatttccc
     1861 gtccgggccc cgga
//
LOCUS       DASPYY010000620         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_384601, whole genome shotgun sequence.
ACCESSION   DASPYY010000620 DASPYY010000000
VERSION     DASPYY010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384601"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEP73_09775"
     CDS             complement(<1..459)
                     /locus_tag="VEP73_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYT26756.1"
                     /translation="MGGFGVESEIGLLRKVLLHKPDLELQRLTPSNVDELLFDEIMWV
                     DRAQAEHEAFAGVLRGRGVEVHELADLLADCMALEPARKELLDRVVTVDMFGKGLCAE
                     LREWFDSLPPAELVRYLIGGVIPEDLPFAPEGLVGASFHPTTFVLPPLPNQ"
     gene            596..1036
                     /locus_tag="VEP73_09780"
     CDS             596..1036
                     /locus_tag="VEP73_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26757.1"
                     /translation="MARHLIVAHQTAASPELAERVRAIADGDPDAEFVLLVPATPTSH
                     LLHTWEEGEARQLAARRGREAAALLSAAGVKVTATRVGCHSPLEAVGDELQARPGYAG
                     IVLSTFPPGVSRWLKGDLVAQLRRRYRLPVVHVVAAPTRAASTG"
     gene            complement(1047..1496)
                     /locus_tag="VEP73_09785"
     CDS             complement(1047..1496)
                     /locus_tag="VEP73_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26758.1"
                     /translation="MTDTAPPGQADRQAPAPRRPKREILGPTGLVVMVAAILAFTAVS
                     IVIELVANSGRGSFHPTVDVLGPVGSSRNEVRVVFHVRNDSRRAGHPDKCEATLYDAK
                     RERVGTASVSVQEDIQPGETLDLLSVGTVASSQVVNGAARCRSLEPD"
BASE COUNT          220 a          610 c          648 g          213 t
ORIGIN      
        1 ctggttcggc agcgggggga gcacgaacgt ggtcgggtgg aagctcgccc cgacgagccc
       61 ctcgggtgcg aacggcaggt cctcggggat gaccccgccg atgaggtagc ggaccagctc
      121 ggccggcggc agcgagtcga accactcgcg cagctcggcg cacaggccct tgccgaacat
      181 gtcgacggtc accacccggt cgaggagctc cttgcgggcc ggctcgagcg ccatgcagtc
      241 ggcgagcagg tcggccagct cgtgcacctc gacgccgcgg ccgcggagca cgccggcgaa
      301 cgcctcgtgc tcggcctggg cgcggtcgac ccacatgatc tcgtcgaaca ggagctcgtc
      361 gacgttggac ggggtcaggc gctgcagctc gaggtctggc ttgtggagca gcaccttgcg
      421 cagcaggccg atctcggact cgacgccgaa cccgcccatg ctcgcccgct ccccctgccg
      481 gtgccgaccg tcgctggcca tcgccccagg ctaccaggcc aagggcgggg ccttgacacc
      541 cgagccgggc aagcgcacaa cacaagggca cgagcccccg aggagggagg acgccatggc
      601 caggcacctc atcgtggccc accagacggc cgccagcccg gagctggccg agcgggtgag
      661 ggcgatcgcc gacggggacc cggacgccga gttcgtcctg ctggtgccgg ccacgccaac
      721 gagccacctg ctgcacacct gggaggaggg cgaggcgcgc cagctcgccg cgcgccgcgg
      781 ccgggaggcg gcggcactgc tctccgccgc cggcgtgaag gtgaccgcga cgagggtcgg
      841 gtgccattcg ccgctcgagg cggtcggcga cgagctccag gccaggcccg ggtatgcggg
      901 catcgtgctc tcgaccttcc cgcccggggt gtcccgctgg ctcaaggggg acctggtcgc
      961 ccagctgcgc cggcggtacc ggctgccggt cgtgcacgtc gtcgccgcgc ccacccgcgc
     1021 ggcgagcacg ggctgaccgc gagcgctcag tccggctcga ggctgcggca gcgtgcggcg
     1081 ccgttcacca cctggctgga ggcgacggtg cccaccgaca gcaggtcgag cgtctcgccc
     1141 ggctggatgt cctcctggac gctgacgctc gcggtgccga cccgctcgcg cttggcgtcg
     1201 tagagggtcg cctcgcactt gtcggggtgg ccggccctcc tgctgtcgtt gcggacgtgg
     1261 aacaccaccc gcacctcgtt ccggctgctg ccgaccggcc cgagcacgtc gacggtcggg
     1321 tggaagctgc cccgcccgct gttggccacg agctcgatca cgatgctcac ggcggtgaag
     1381 gcgaggatcg cggccaccat gacgacgagg ccggtggggc cgaggatctc gcgcttgggg
     1441 cggcggggtg cgggggcctg gcggtcggcc tggccgggcg gagcggtgtc ggtcacgatg
     1501 gcgtcccggt ggcgtggcgg ttgtcgctgg ctgcggtggc gcccgcgccg gacgggcggc
     1561 gggcgccgcc acactcaatc tagcagggcc tcgccgccgc cgcgccggct accgcagtcg
     1621 cgctggcggc ccggctaccg cagaccgggc agggacacgg tgaccgtcac cccgggcagg
     1681 gagatgggcg g
//
LOCUS       DASPYY010000621         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_807076, whole genome shotgun sequence.
ACCESSION   DASPYY010000621 DASPYY010000000
VERSION     DASPYY010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807076"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="VEP73_09790"
     CDS             complement(<1..1986)
                     /locus_tag="VEP73_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYT26759.1"
                     /translation="MPPGDVRSVEIVGRAASPACVLDPNGRIVAANRTLEELLGSGTR
                     GLRGRSLTSLAEPRDVELVRGALAAVFVEGLDRHLVEQRCVRVDNRQLVWGAWDFSAV
                     RDTRGRPELAIAMVRDRTEAMRSENERRFFEFLLKIIGEAEDSDAMLAAAVQTVCHFT
                     RCAMGQTWVPLDDSLVCSPSWHVNGYGFDQLRSVSEGIRYERGAGLPGHVWRTGQPCL
                     LREIHDPARFDRAVAAGRAGAGEVMAVPIQTQSGLVAVLELFVTYERAGEPGRLERIS
                     RAVAELGPVIERKRAEEALRASEERFRGVTGAAMDAIVSADGEGRLRSWNRGAERMFG
                     WQADEVIGKPLTVIVPERFRALHAEGIARVGRTGRSKLAGSVVELTGLHRDGREFPIE
                     LSIGAWTSSEGLAFSGVMRDISERKRAEEALAAAHRELERTNAELETLVYSASHDLKS
                     PMVSLLGYLEYLKLDYGDVLGEDGNRYVERMSHCTLYMQRLIHDLIDLSRVGRLADVA
                     ADVDLAELVRTIADEASSTHPAVRFHVGPLPVVTADPVGFRQLYTNLVENAISHGGRP
                     DLAVTVTATSRVDGSLELSVADDGKGIPPEHRERVFGVFERLDGPSSSSGTGMGLAIC
                     RKIVELLGGSIGIHGTEGTEVRIVLPGGVVARWPEAER"
     gene            complement(2191..2418)
                     /locus_tag="VEP73_09795"
     CDS             complement(2191..2418)
                     /locus_tag="VEP73_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYT26760.1"
                     /translation="MPTRLSDDDAKLLEEANYGHFATLMPDGSPQVTPVWADTRFLAR
                     KYLGVESYPNRVPGERRVIVRIQPEHVARMR"
     gene            2613..3494
                     /locus_tag="VEP73_09800"
     CDS             2613..3494
                     /locus_tag="VEP73_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26761.1"
                     /translation="MALHVMMISRDPELVTGLATSLEEPDRLTVVDSPDAVTDHGTEV
                     DAVVVDLPADGRQAACERVRERYQGRLVVPVDDGGEVAGWSPDPARLVLVRPFPVADL
                     LACLEPGGVSSDEQAVAARRQVRRRPQGLIRPAGAEALDPAFGAPPGPLGGVAAGPAD
                     GVASPDGAAAAPPAGPAPRRLSPDEPIWDPPAVFAPRGALARVGTPGTVVERRQDPAG
                     PPAGAGRRSGRRIALMVVAAIGLLLTGALAGAGLAQAARAPGGRSASRAATAAATAPP
                     ATAAPAEAAHARTDDDA"
BASE COUNT          454 a         1350 c         1273 g          465 t
ORIGIN      
        1 ccgctcggcc tcgggccagc gggcgacgac gccgcctggc aggacgatcc gcacctccgt
       61 gccctcggtg ccgtggatgc cgatgctccc gccgagcagc tccacgatct tgcggcagat
      121 cgccaggccc atgccggtcc cgctgctgga cgagggcccg tcgaggcgct cgaacacgcc
      181 gaacacgcgc tcccggtgct cgggcgggat ccccttgccg tcgtcggcga ccgacagctc
      241 gagcgagccg tccacgcggc tcgtggccgt gaccgtcacc gcgaggtcag gcctgccgcc
      301 gtggctgatc gcgttctcca cgaggttggt gtagagctgc ctgaagccca ccgggtcggc
      361 ggtgacgacc ggcagcgggc cgacgtggaa gcggaccgcc ggatgggtcg aggacgcctc
      421 gtcggcgatc gtgcggacga gctcggcgag gtcgacgtcg gcggcgacgt cggcgaggcg
      481 gccgacgcgc gacaggtcga tcaggtcgtg gatcagccgc tgcatgtaca gggtgcagtg
      541 ggacatgcgc tcgacgtagc ggttgccgtc ctcaccgagc acgtcgccgt agtcgagctt
      601 caggtactcg aggtagccga gcagggagac catcgggctc ttcaggtcgt gcgaggcgct
      661 gtacaccagg gtctccagct cggcgttggt ccgctcgagc tcccggtggg cggctgcgag
      721 cgcctcctcc gcccgcttcc gctcggagat gtcgcgcatc acgcccgaga acgccagccc
      781 ctcgctcgac gtccacgccc cgatcgacag ctcgatgggg aactcccggc cgtcgcggtg
      841 cagcccggtc agctccacca cgctgccggc cagcttcgag cggcccgtcc tgccgacccg
      901 cgcgatcccc tcggcgtgca gcgcccggaa ccgctcgggg acgatcaccg tcagcggctt
      961 gccgatcacc tcgtcggcct gccagccgaa catccgctcg gcgccccggt tccaggagcg
     1021 cagccgcccc tcgccgtcgg ccgacacgat cgcgtccatg gccgcgccgg tcaccccccg
     1081 gaaccgctcc tccgacgccc gcagcgcctc ctccgcccgc ttccgctcga tgacggggcc
     1141 gagctcggcg acggcccggg agatgcgctc gaggcgtccc ggctccccgg cccgctcgta
     1201 ggtcacgaag agctccaaga ccgccacgag tccggactgg gtctggatgg ggaccgccat
     1261 gacctcgccc gcgccggccc ggcccgcggc gacggcccgg tcgaaccggg ccgggtcgtg
     1321 gatctcccgc agcaggcagg gctggccggt cctccagacg tggccgggga gcccggcgcc
     1381 gcgctcgtag cggatcccct cgctcacgga gcggagctgg tcgaagccgt acccgttgac
     1441 gtgccaggag ggactgcaca ccagcgagtc gtcgagcggg acccaggtct gccccatggc
     1501 gcacctggtg aagtggcaga cggtctggac ggccgccgcc agcatggcgt cggagtcctc
     1561 cgcctcgccg atgatcttga gcaggaactc gaagaaccgg cgctcgttct cggagcgcat
     1621 ggcctcggta cggtcgcgca ccatggcgat ggcgagctca ggccgccccc gggtgtcgcg
     1681 gaccgccgag aagtcccagg cgccccacac cagctgccgg ttgtcgaccc ggacgcagcg
     1741 ctgctcgacg aggtggcggt cgaggccctc gacgaagacc gccgccagcg cgccgcggac
     1801 gagctccacg tcgcgcggct cggccagcga cgtgagcgac cggccgcgga gccctctcgt
     1861 cccggacccg agcagctcct cgagggtccg gttggcggcg acgatgcggc cattcgggtc
     1921 gagcacgcac gccggcgagg ccgcccgacc gacgatctcg accgaccgga cgtccccagg
     1981 cggcatccgc cacctggagc gccgccttct ccccgcgcgc ccgctgaccc gcatcgcgac
     2041 accccccaca cccgtaccgg ccgcccgtag cggaccgtac gtctgaatac cgctatttgg
     2101 gtgcctgtat atcatcgcgt cgatcgcatg gcgaaaccga gaaagcgccc ttgcacgccg
     2161 gaccgggacg cgcccgcacg gcagcgccgg ctacctcatg cgggcgacgt gctccggctg
     2221 gatcctgacg atcacgcgcc gctcgccggg cacgcggttg gggtagctct cgacgccgag
     2281 gtacttcctg gcgaggaagc gggtgtcggc ccagaccggc gtgacctgcg gcgagccgtc
     2341 cggcatcagc gtggcgaaat gcccgtagtt ggcctcctcg agcagcttgg cgtcgtcgtc
     2401 gctcagcctc gtcggcaaga tgcctgcctc ctcgtcgtcg ggctgtcagc catccggaat
     2461 gaccacttgg aatggcaacc gaggccgccg gccaaacgtc ccggaacggt gcgaggttta
     2521 cggcctgacg gcgcggagac ctacaattgg tggcgcggcc ggcacggagc ccgtgagggc
     2581 cggacctgtg tctgggaggg gggaggcggc ccatggccct ccatgtgatg atgatctcca
     2641 gggatcccga gctcgtgacg ggcctggcca cgtccctcga ggaacccgac cggctcacgg
     2701 tggtcgactc gccggacgcg gtgaccgacc acgggaccga ggtcgacgcc gtcgtcgtcg
     2761 acctgccggc cgacgggagg caggcggcgt gcgagcgggt cagggagcgc taccagggcc
     2821 ggctcgtcgt gccagtagat gacggcggcg aggtggcggg ctggtcgccc gacccggccc
     2881 ggctcgtcct cgtccgcccg ttcccggtcg ccgacctcct ggcctgcctc gagccgggcg
     2941 gcgtgtcctc cgacgagcag gcggtcgccg cccgccgcca ggtccgccgg cggccgcagg
     3001 gtctgatccg gccggccggc gccgaggcgc tcgacccggc cttcggcgcc ccgcccgggc
     3061 cgctcggcgg ggtggcggcc gggccggccg acggcgtggc atcgccggac ggggcggcgg
     3121 ccgccccgcc ggctggaccg gcgcccagac ggctctcgcc cgacgagccg atctgggacc
     3181 cgccggccgt gttcgccccg cgcggcgctc tggccagggt gggcaccccc ggcacggtcg
     3241 tggagcggcg ccaggacccc gcgggaccgc cggccggcgc cgggcggcga tccggccggc
     3301 ggatcgcgct catggtcgtc gcggcgatcg ggctgctcct caccggcgcg ctcgccggtg
     3361 ccggcctcgc ccaggctgcc agggcccctg gcgggcggtc ggcgtcccgc gcggcgaccg
     3421 ccgcggccac cgccccgccg gccaccgccg ccccggccga agccgcccac gcccggactg
     3481 atgatgatgc ctagcgcatc gagttcctca cgcagcgctt gccggtcgtg cttcttggtg
     3541 cc
//
LOCUS       DASPYY010000622         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1932396, whole genome shotgun sequence.
ACCESSION   DASPYY010000622 DASPYY010000000
VERSION     DASPYY010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932396"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VEP73_09805"
     CDS             <1..273
                     /locus_tag="VEP73_09805"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HYT26762.1"
                     /translation="AVPVGPLARTGSGRWQFFTVPSGQPSRILWGPSSRHAVRWHSRG
                     GFAVAPPSRHPGGAVAQWVRPLATPLPDQRPLVHELLAARNAHGAA"
     gene            530..1027
                     /locus_tag="VEP73_09810"
     CDS             530..1027
                     /locus_tag="VEP73_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26763.1"
                     /translation="MPAFFLLLAIVGGIAVGDLVLENTSRGEVTVLNQAITGYTEGQL
                     LAAAAALGFVVALLLAASVSAARARRVRRTRLHTATRNSRGRMPEPEGGREHLSPRDE
                     HARPADLERDRWVDEPQDRRAATAPQQVKRSPEPLYEESRRAARLHHDPDLWSPRHTD
                     DRRTP"
     gene            complement(1104..>1897)
                     /locus_tag="VEP73_09815"
     CDS             complement(1104..>1897)
                     /locus_tag="VEP73_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HYT26764.1"
                     /translation="GAVVGNAPTAPASTITATLTLVFPCYNEAGRLPRTLGAYLDRLP
                     RTSGQVEVLVVDDGSTDGTSAVASTIAAGDDRVRVIRSEFNRGKGFAVRTGMLAAGGE
                     LVVFTDADGSYRPDDVERIAAALADAPVAIGSRDLRAQASSRTRQLASRLFNHAMRTL
                     LDLPFRDTQCGLKGFRRQAALEVFGRARLDGFAFDAEVLYLARRLGLEVAEVVVRAEE
                     RDGSKVRVAVDALAMLREALAVRRAVAAGAYDRDIRGGLYADPTP"
BASE COUNT          255 a          723 c          681 g          238 t
ORIGIN      
        1 gccgtcccgg tgggacccct ggcgcggacg ggcagcggcc gctggcagtt cttcaccgtc
       61 cccagcgggc agcccagcag gatcctgtgg gggccgagct cgcggcacgc ggtccgctgg
      121 cacagccgcg gcggcttcgc cgtcgccccg ccgagccggc accccggcgg cgcggtggcg
      181 caatgggtgc gcccgctcgc caccccgctc cccgaccagc ggccgctcgt gcacgagctc
      241 ctcgccgcgc gcaacgcgca tggtgcggcc tgaccggccg ggcgtcgtga cgtagcgccc
      301 acccgtggta gtctcccccc aagatacctt ggccggtgcc gccctccccc ctccgcgggc
      361 agtaccggcc acagaccgca agcccctcac cgcaggctgg tgacgtctga cgtttcccac
      421 tatccgaccg tccggaccga gcctggtgct gttctgccct ggcacgccag cggcgaccac
      481 agcacgtgcg gacgtcaggc caccccgact ctgactctgg aggttgccag tgcccgcgtt
      541 cttcctcctt ctggcgatcg tcggcggcat cgccgtgggc gacctcgtgc tggagaacac
      601 cagccgcggc gaggtcacgg tgctcaatca ggcgatcacc ggctacaccg agggccagct
      661 gctggcggcg gccgcggcgc tcggcttcgt cgttgccctg cttctggccg cctcggtgag
      721 tgcggcgaga gcccgccgcg tccgccgtac gcggctgcat acggccacgc gcaactccag
      781 gggccggatg cccgagcccg agggcggacg cgagcacctg agcccgcggg acgagcacgc
      841 ccggccggcg gacctggagc gcgaccgctg ggtggatgag ccacaggacc gccgcgccgc
      901 gaccgcgccg cagcaggtca agcgctctcc tgagccgctc tacgaagaga gcaggcgggc
      961 cgcccggctg caccacgacc cggacctctg gtcaccgcgg cacaccgacg acaggcgtac
     1021 cccgtagcat cgagggtctg gcacccggtg caggaaacgg tgaggggcgc ccgcgggcgc
     1081 ccctcaccgt ttcctgcacc gggtcacggc gtcgggtcgg cgtacaaacc gccccggatg
     1141 tcgcggtcgt aggcgccggc cgcgaccgct cgccgcaccg cgagcgcctc ccgcagcatc
     1201 gcgagcgcgt cgaccgcgac ccgcaccttg ctgccgtccc gctcctcggc ccggacgacg
     1261 acctcggcca cctcgagacc caggcggcgg gcgaggtaca gcacctcggc gtcgaacgcg
     1321 aagccgtcga gcctggcgcg gccgaacacc tccagcgcgg cctgacggcg gaaccccttg
     1381 aggccgcact gggtgtcgcg gaacggcagg tcgagcagtg tccgcatggc gtggttgaac
     1441 agcctgctgg cgagctggcg cgtgcgtgag ctggcctggg cgcggaggtc ccgggagccg
     1501 atcgcgaccg gcgcgtcggc cagcgcggcc gcgatccgct ccacgtcgtc aggccggtag
     1561 gagccgtcgg cgtcggtgaa cacgacgagt tccccgccgg ccgcgagcat gcccgtccgc
     1621 acggcgaatc ccttgccgcg gttgaactcg gaacggatca cccggacacg atcgtcgccg
     1681 gccgcgatgg tgctcgccac ggcggaggtg ccgtcggtcg agccgtcgtc cacgacgagc
     1741 acctcgacct gaccggacgt ccgtggcagg cggtcgagat aggcgccgag cgtgcgcggc
     1801 aggcggccgg cctcgttgta gcaggggaag accagcgtga gggttgccgt gatggtggac
     1861 gcgggcgccg tcggagcgtt gcccacgacg gcgcccg
//
LOCUS       DASPYY010000623         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1088229, whole genome shotgun sequence.
ACCESSION   DASPYY010000623 DASPYY010000000
VERSION     DASPYY010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088229"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VEP73_09820"
     CDS             <1..537
                     /locus_tag="VEP73_09820"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYT26765.1"
                     /translation="FILADARGKGLEASSLASVLKGAFRSLAGEGASPARVLGRLDRV
                     VAREGGDEDFVTALAGRAHPDGRIVLATAGHPFPLGAAPRPGEVGAPLGLGTRAAEAQ
                     ATLCAGQRLVCYTDGLVEARNQAGEFIDRAAFEQVVHVEPLDAALDHLVAMVNDHSVD
                     YRADDLALLGLEYAPAGP"
     gene            610..1758
                     /locus_tag="VEP73_09825"
     CDS             610..1758
                     /locus_tag="VEP73_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT26766.1"
                     /translation="MDFELAPEQQEFAKIVREFAEDVVAPRAEAMDRAEELDEAVLDQ
                     MAAMGLFGLPFPEAYGGMGADYLTLCLAIEELARVDSSVAITLEAAVSLGAMPIYRSG
                     TEEQKQRWLRPLATGERIAAFGLTEPGGGSDAGSLRTRAVRDGDEWVLDGTKAFITNA
                     GLPRSGLVTVAAATGGRPDGRPEITNVIVPTGTPGYTVGRRYSKVGWHASDTRELSFE
                     GCRVPVANTLGERGTGLANFLRALEEGRIAVAALATGLCQGCVDECVRYARERHAFGR
                     PISSFQAISFKIADMELRAHTSRLAYQHAAARLVAGQPFGRQAAVAKLHASEAAVACA
                     REAVQVHGGYGYMNEFPVARFYRDAKILEIGEGTSEVMRILIARSLGL"
     gene            complement(1721..1912)
                     /locus_tag="VEP73_09830"
     CDS             complement(1721..1912)
                     /locus_tag="VEP73_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26767.1"
                     /translation="MVAWFPLATDEGVRNQVAGITVALLWLLVVEGLLVGLLPSVGKW
                     LFSGAGRLRARASGRSGCA"
     gene            2010..>2359
                     /locus_tag="VEP73_09835"
     CDS             2010..>2359
                     /locus_tag="VEP73_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26768.1"
                     /translation="MVRARPPQEPPGSVALFARCVRGTEWVAAAELAGRLGARAVATG
                     HREVRFRLPGALAPALDLRTVDDVFLHVASLDGIDHTRASLGVLAGAAGTLDARDAVE
                     RVRALRRTAGPLAM"
BASE COUNT          301 a          907 c          864 g          287 t
ORIGIN      
        1 ttcatcctgg ccgacgcccg cggcaagggc ctcgaggcga gctcgctcgc gtcggtgctc
       61 aagggcgcct tccgcagcct ggccggggag ggcgccagcc cggcgcgcgt gctcggccgg
      121 ctcgaccggg tggtcgcccg cgaagggggg gacgaggact tcgtgaccgc gctggccggc
      181 cgggcccacc cggacggccg gatcgtcctc gccaccgccg gccacccgtt cccgctcggc
      241 gccgcgccgc gtcccggcga ggtcggggcg ccgctcggcc tcggcacccg ggccgccgag
      301 gcgcaggcca ccctgtgcgc gggccagcgc ctcgtctgct acaccgatgg cctggtcgag
      361 gcgcgcaacc aggcgggcga gttcatcgac cgggccgcgt tcgagcaggt cgtgcatgtc
      421 gagccgctcg acgccgccct cgaccacctg gtcgcgatgg tcaacgacca cagcgtcgac
      481 taccgggccg acgacctggc gctcctcggt ctcgagtacg cccccgccgg cccctgaccc
      541 cggggggcac tagcatggcg ggagcgccac cagcgcaccc gcacatgacg ccccacgagg
      601 gagccgacga tggacttcga gctcgcaccc gaacagcagg agttcgcgaa gatcgtccgc
      661 gagttcgccg aggacgtggt cgcgccgagg gccgaggcga tggaccgcgc cgaggagctg
      721 gacgaggccg tgctcgacca gatggccgcg atgggcctgt tcggcctgcc gttcccggag
      781 gcatacggcg gcatgggagc cgactacctg accctgtgcc tcgcgatcga ggagctggcc
      841 agggtcgact cgtcggtcgc gatcaccctg gaggccgccg tcagcctcgg cgccatgccg
      901 atctaccggt ccgggaccga ggagcagaag cagcgctggc tgcggcccct cgccaccggc
      961 gagcggatcg cggcgttcgg gctcaccgag ccggggggcg gctcggacgc ggggtcgctc
     1021 aggaccaggg ccgtgcggga cggcgacgag tgggtcctcg acggcaccaa ggcgttcatc
     1081 accaacgccg ggctgccgcg ctcgggcctc gtcaccgtcg ccgccgcgac cggcgggcgg
     1141 cccgacggcc gcccggagat caccaacgtc atcgtgccga ccggcacgcc cgggtacacc
     1201 gtgggcaggc ggtactccaa ggtcggctgg cacgcgtcgg acacccggga gctctcgttc
     1261 gagggctgcc gggtcccggt cgccaacacg ctcggggagc gggggaccgg cctcgcgaac
     1321 ttcctccgcg cgctggagga gggccgcatc gcggtcgcgg cactcgccac cgggctgtgc
     1381 cagggctgcg tcgacgagtg cgtccgctac gctcgcgagc gccacgcgtt cggccgcccg
     1441 atctcgtcgt tccaggccat ctcgttcaag atcgccgaca tggagctgcg cgcgcacacc
     1501 tcccgcctcg cctaccagca cgccgcggcc aggctggtcg ccgggcagcc gttcggcagg
     1561 caggcggcgg tcgccaagct ccacgcgtcc gaggcggccg tcgcctgcgc ccgcgaggcg
     1621 gtccaggtcc acggcggcta cggctacatg aacgagttcc cggtcgcccg cttctaccgc
     1681 gacgcgaaga tcctcgagat cggggagggg accagcgagg tcatgcgcat cctgatcgcc
     1741 cgctcgctcg ggctctgagc ctcccggcgc cgctgaagag ccacttgccg accgacggca
     1801 ggagcccgac gagcaggccc tcgaccacga ggagccagag cagcgcgacg gtgatgcccg
     1861 cgacctggtt gcgcacgccc tcgtcggtcg cgagggggaa ccaggcgacc atgccgagcc
     1921 gtccgtcccc ggggcggggg agtggccggg cgccccggcg gcatactgtc gccgcaacgc
     1981 cctgatcttg accacccctc gggaggaccg tggtccgcgc ccgcccaccg caggagcccc
     2041 ccggctccgt cgcgctcttc gcccgctgcg tgcgcggcac ggagtgggtc gccgccgccg
     2101 agctggccgg ccgcctcggc gctcgtgccg tcgcgaccgg ccaccgcgag gtgcgcttcc
     2161 gcctgccggg cgcgctcgcg ccagcgctcg acctgcgcac ggtcgacgac gtgttcctcc
     2221 acgtcgcgtc ccttgacggg atcgaccaca cccgggccag cctgggcgtg ctcgccggcg
     2281 cggccggcac gctagatgcg cgggacgccg tggagcgggt ccgcgccctg cggcgcaccg
     2341 ccgggccgct cgccatgga
//
LOCUS       DASPYY010000624         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_384670, whole genome shotgun sequence.
ACCESSION   DASPYY010000624 DASPYY010000000
VERSION     DASPYY010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384670"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VEP73_09840"
     CDS             <1..1283
                     /locus_tag="VEP73_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYT26769.1"
                     /translation="GRIPLFREFARLLAGGTGPVNWELARQVAVATAAGADALGTTPE
                     LPTPSQAPPASEQREWDDRLRLAELWVEPATTLAGHGLTLTARPVSRPDWAETVLPGF
                     APLVEPGARRVSEALGSGTVAGMPAEMGDVMGRLGSLLTGLQVGTLVGQLARLVLAQY
                     DLALPAADAGRVLVLAENVREFEQASGLPADQFRLWLACHEVVRQRLLCGVPWMADHL
                     RGVVDEIARQTEPDASGLMDRLQSLDLGRLESLQEVLEGDQDVLGPASPGLQAAIGRL
                     EILLALTEGYADVICARALAGRLPALAAIEAALGRRLADLEGAHAQFAQLLRADPGRS
                     AAANGQRFCREVLAATDIDGLDRLWAHPDFLPTPEELAVPGRWLERSGLVGGDEVDLD
                     EGLRALLEGEPGPGGDDARGTGDGADGAEGSPGR"
     gene            complement(1352..>1995)
                     /locus_tag="VEP73_09845"
     CDS             complement(1352..>1995)
                     /locus_tag="VEP73_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYT26770.1"
                     /translation="FLLGVAGIVYEVVHPGIGVGGVGGALALIVALLEFQALPVELGG
                     LVLIALGIALFVLDLKLAGHAFLSVFGVISLVLGGLLLYEPGSSARVHPVVLVLVVAC
                     VAALFLVVARKVLAARRERPVTGVDALLGVPGTVVAELAPEGRVRAAGIEWRARAAPG
                     GTLPAGTQVVVTGLDGLTLEVERAPAPELGSPFPAAPTAPATPATRGRRRRPR"
BASE COUNT          241 a          763 c          774 g          217 t
ORIGIN      
        1 tcggccgcat cccgctgttc cgcgagttcg cgcggctcct ggccggtggt accgggccgg
       61 tcaactggga gctggcccgg caggtcgcgg ttgccaccgc ggccggcgcc gacgcgctcg
      121 gcaccacccc cgagctcccc acgccctccc aggctccgcc cgcgagcgag cagcgggagt
      181 gggacgaccg cctgcgcctg gctgagctgt gggtcgagcc ggccacgacc ctggccggtc
      241 acggcctcac cctcaccgcg aggccggtga gccgccccga ctgggccgag acggtcctgc
      301 ccggcttcgc gccactggtc gagccgggcg cccgccgggt cagcgaggcg ctgggctcgg
      361 gcacggtggc cggcatgccg gccgagatgg gcgacgtcat gggccgcctg gggagcctcc
      421 tgaccggcct gcaggtcggg acgctggtcg gccagctcgc ccggctggtc ctcgcccagt
      481 acgacctcgc gctgcccgcc gccgacgccg gtcgcgtgct cgtcctggca gagaacgtgc
      541 gcgagttcga gcaggcgtcc ggcctgccgg ccgaccagtt ccggctgtgg ctcgcctgcc
      601 acgaggtggt ccgccagcgg ctgctctgcg gggtgccctg gatggccgac cacctgcgcg
      661 gggtcgtcga cgagatcgcc aggcagaccg agccggacgc ctccggcctg atggaccggc
      721 tccagtcgct cgacctcggc cggctcgaga gcctccagga ggtgctggag ggcgaccagg
      781 acgtgctcgg cccggcgagc cccggcctgc aggccgccat cgggcggctg gagatcctgc
      841 tcgccctcac cgagggctac gcggacgtga tctgcgcgcg ggcccttgcc ggtcgcctgc
      901 ccgccctggc ggcgatcgag gcggcgctcg gccggcggct cgccgacctg gagggcgccc
      961 acgcgcagtt cgcgcagctg ctgcgggccg acccgggccg gtcggccgct gccaacgggc
     1021 agcgcttctg ccgggaggtg ctggccgcga ccgacatcga cgggctcgac cggctctggg
     1081 cccatcccga cttcctcccc accccggagg agctggccgt ccccggccgc tggctcgagc
     1141 ggagcggtct cgtcggcggc gacgaggtcg acttggacga gggcctgcgc gcgctgctcg
     1201 aaggcgagcc gggtcccggg ggcgacgacg cgcgggggac gggcgacggc gcggacggcg
     1261 ccgagggctc ccccgggcgc tgaccgcgct cccgtcgcgg tcgacgcggc ggcatccgac
     1321 cggcggacct cggccccgcc gcggagggat ctcagcgcgg gcggcgccgg cggccgcgcg
     1381 ttgctggggt tgctggcgct gtgggcgcgg ccgggaacgg cgatcccagc tcgggcgctg
     1441 gcgcccgctc gacctccagc gtgaggccgt ccaggccggt cacgaccacc tgggtccccg
     1501 cgggcagggt gccgccgggg gctgcccgcg cccgccactc gatcccggcc gcgcggaccc
     1561 ggccctcggg ggccagctcg gcgaccaccg tgcctggcac cccgaggagc gcgtcgaccc
     1621 cggtgacggg ccgctcccgt ctcgcggcga ggaccttgcg ggccacgacc aggaacaggg
     1681 cggccacgca ggcgaccacg aggacgagca cgaccgggtg cacccgggcc gaggacccgg
     1741 gctcgtagag caggaggccg ccgagcacga gggagatcac gccgaagacg gacaggaagg
     1801 cgtgcccggc gagcttgagg tcgagcacga agagggcgat gccgagcgcg atgaggacga
     1861 gcccgccgag ctccaccggg agcgcctgga actcgagcag ggccacgatc agcgcgagcg
     1921 cgccgcccac cccgcccacg ccgatccccg gatggacgac ctcgtagacg atcccggcga
     1981 cgccgaggag gaaca
//
LOCUS       DASPYY010000625         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_103467, whole genome shotgun sequence.
ACCESSION   DASPYY010000625 DASPYY010000000
VERSION     DASPYY010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103467"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1230
                     /locus_tag="VEP73_09850"
     CDS             40..1230
                     /locus_tag="VEP73_09850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYT26771.1"
                     /translation="MGERSVVIASARRTPMGRFMGAFADVPAVELGVVAARAALEAAG
                     LDPGEVQETVAGQGRQAGNGPNPGRQVSVRAGVPETVPAYTVNKACGSSTKALALAAG
                     SILLDEIDVALVVGMENMTRTPYLLRGMRGGYRMGDATIEDGMSSDGFMDPLAGIGMG
                     ETAENLAERYGVGRQEQDAFALRSQRRAGAARAAGRLEVEVTAVETTDARSRTVTVDR
                     DEQPRPDTTMDALARLRPAFRTPGTVTAGNSSGICDGAAALVVMTGDAARVRGVEPLA
                     RVVGWASAGVDPAVMGWGVVPATQKLLARIGWTLPEIDVVEVNEAFAAQVLACDRELK
                     FDWERTNVNGGAIALGHPIGMTGARIVGAVAHELRRRDARRGLATLCISGGQGMAVAL
                     ERAG"
     gene            1316..1615
                     /locus_tag="VEP73_09855"
     CDS             1316..1615
                     /locus_tag="VEP73_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYT26772.1"
                     /translation="MVTAIVLVTCEVDRTPETAQALADLDGVSEVYSVAGDWDLVAVV
                     RVGSHDDLASVVTEHIRKVGGVTTTQTMIAFRTYSRHDLDELFSVGFAADAGQPD"
     gene            1619..2206
                     /locus_tag="VEP73_09860"
     CDS             1619..2206
                     /locus_tag="VEP73_09860"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYT26773.1"
                     /translation="MGFQAPDEHRAGDLLLRSYRPGDGPELNRATVSSYRHLRRFMPW
                     ARADTTDEHGEETARRFRAAWLLGEREWVVGVWRGAKLAAGTGFHLHGRPLDEGVADV
                     GMWVRADEAGRGLGTRVLEAMVDWADGAWPFYRLTWGCDARNVASARVAEKCGFVLEG
                     RLREDAPDHLDPAHRRDTLLFGLLPADPRPWRHRG"
     gene            complement(2261..>2860)
                     /gene="trpD"
                     /locus_tag="VEP73_09865"
     CDS             complement(2261..>2860)
                     /gene="trpD"
                     /locus_tag="VEP73_09865"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYT26774.1"
                     /translation="ETGIGFCFAPLFHPALRHAAAPRRELGVPTVFNLLGPLANPARA
                     RHQALGVADPLMVPVMAEVLRRTGTRHALVFHGHDGLDELTTTTTSMLLDVSQDGVRE
                     MEVDPVALGLAPATRESLVGGDVERNVAIAKQVLAGEGGPPRDLVLLNTAAALVAADA
                     ALSLVDGLEAAARSIDEGRAAAVLEAWVEASRRAAAGRA"
BASE COUNT          367 a         1093 c         1071 g          329 t
ORIGIN      
        1 cctacaatga gctcgtcgag ccagggtgga gggcagcgaa tgggagagcg atccgtcgtc
       61 atcgcgtcgg ccaggcgcac cccgatgggc aggttcatgg gcgccttcgc cgacgtgcca
      121 gcggtcgagc tcggcgtcgt cgccgcccgg gcggcgctgg aggcggccgg cctcgaccct
      181 ggggaggtcc aggagacggt cgccggccag ggcaggcagg ccggcaacgg gccgaacccc
      241 ggccggcagg tgtcggtccg ggccggcgtg cccgagacgg tccccgccta cacggtcaac
      301 aaggcgtgcg ggtcgagcac caaggcgctc gccctcgccg cgggctcgat cctcctggac
      361 gagatcgatg tcgccctggt cgtcggcatg gagaacatga cccgcacgcc gtacctgctg
      421 cgcggcatgc gcggcggcta ccgcatgggc gacgcgacga tcgaggacgg catgtcgtcg
      481 gacggcttca tggatccgct ggccggcatc ggcatgggcg agacggccga gaacctcgcc
      541 gagcgctacg gcgtcggccg ccaggagcag gacgccttcg cgctccggtc gcagcggcgg
      601 gcgggcgccg cccgggccgc gggccggctc gaggtcgagg tcaccgccgt cgagacgacg
      661 gacgccaggt cccgcaccgt gaccgtcgac cgcgacgagc agccccgccc cgacacgacc
      721 atggacgccc tcgcccggct gcggccggcc ttccggacgc cgggcaccgt gaccgccggc
      781 aactcctcgg gcatctgcga cggcgccgcc gccctcgtcg tcatgaccgg ggacgccgcc
      841 cgggtgcggg gcgtcgagcc gctcgcgcgg gtcgtcggct gggccagcgc cggggtggac
      901 ccggccgtga tgggctgggg ggtcgtcccc gccacccaga agctgctcgc caggatcggc
      961 tggacgctcc cggagatcga cgtcgtggag gtcaacgagg cgttcgccgc gcaggtgctc
     1021 gcctgcgacc gcgagctcaa gttcgactgg gagcgcacca acgtcaacgg tggcgccatc
     1081 gcactcggcc acccgatcgg gatgaccggg gcccgcatcg tcggggcggt cgcccacgag
     1141 ctacggcgcc gggacgcgcg gcgcgggctg gccacgctct gcatctccgg cggccagggc
     1201 atggccgtcg ccctcgagcg ggccggctga cggcccgggc ggccgcgcgg cgcaggcacg
     1261 ccggcacgac caagcgccgt cgaccgtaga cggcaccgac cgaagggagc gccagatggt
     1321 caccgcgatc gtgctcgtca cctgcgaggt cgaccgcacc ccagagacgg cccaggccct
     1381 cgccgacctg gacggcgtca gcgaggtgta ctccgtcgcc ggggactggg acctcgttgc
     1441 cgtcgtccgc gtcggcagcc acgacgacct cgcgtcggtc gtcaccgagc acatccgcaa
     1501 ggtgggcggg gtgaccacga cccagacgat gatcgcgttc cggacctact cccgccacga
     1561 cctcgacgag ctgttctcgg tcggcttcgc ggccgacgcc gggcagccgg actgagggat
     1621 gggcttccag gcgcccgacg agcatcgcgc cggcgacctc ctgctcaggt cctaccggcc
     1681 cggggacggg cccgagctga accgcgccac ggtctcctcc taccggcacc tgcgccggtt
     1741 catgccgtgg gcccgcgcgg acaccaccga cgagcacggc gaggagacgg cgcgccgctt
     1801 ccgcgcggcc tggctgctcg gcgagcggga gtgggtcgtc ggcgtctggc gcggggcgaa
     1861 gctcgccgcc ggcaccggct tccacctcca cggccggccc ctcgacgagg gcgtcgccga
     1921 cgtcggcatg tgggtccgcg ccgacgaggc cggccgcggg ctcggcacca gggtgctcga
     1981 ggcgatggtc gactgggccg acggcgcgtg gccgttctac cggctcacct ggggctgcga
     2041 cgcgaggaac gtcgcctcgg ccagggtcgc ggagaagtgc gggttcgtgc tggagggccg
     2101 cctcagggag gacgcccccg accacctcga cccggcccac cggcgcgaca cgctgctgtt
     2161 cgggctcctg ccggccgacc cccggccctg gcgccaccgg ggctgacgca ccaccggagc
     2221 cggctggccg acgcacccgg ccggagccgg gcggcgccgc tcaggcccgg ccggcggccg
     2281 cgcggcgcga cgcctccacc cacgcctcga gcaccgccgc ggcccgcccc tcgtcgatgg
     2341 agcgggcggc cgcctcgagg ccgtccacca ggctgagcgc cgcgtcggcg gcgaccagcg
     2401 ccgccgccgt gttgagcagc accaggtcgc ggggcgggcc gccctccccc gcgagcacct
     2461 gcttcgcgat cgcgacgttg cgctcgacgt ccccgccgac caggctctcc cgggtcgccg
     2521 gggccaggcc gagcgccacc gggtccacct ccatctcccg cacgccgtcc tggctgacgt
     2581 cgagcagcat ggacgtggtg gtcgtggtca gctcgtccag cccgtcgtgc ccgtggaaga
     2641 ccagcgcgtg cctggtgccg gtgcgccgca ggacctccgc catgaccggc accatcagcg
     2701 ggtcggcgac cccgagcgcc tgatgcctgg cgcgcgccgg gttggcgagc gggccgagga
     2761 ggttgaacac cgtcggcacc ccgagctccc gccgcggcgc cgccgcgtgc cgcagggccg
     2821 gatggaacag cggcgcgaag cagaagccga tccctgtctc
//
LOCUS       DASPYY010000626         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_245738, whole genome shotgun sequence.
ACCESSION   DASPYY010000626 DASPYY010000000
VERSION     DASPYY010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245738"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VEP73_09870"
     CDS             <1..700
                     /locus_tag="VEP73_09870"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HYT26775.1"
                     /translation="EAGGGIVEELIRAPGVVSPSVQLRIAPGGEVELVSTHSQLLGGP
                     GGQVYLGCRFPADNAYRLDIQRWALRVGELLAARGVIGWLGVDFIVVPSPSGVETYLS
                     EINLRIGGTTHPLWMALLASEGRYEPASGRLLAAGRAKSYVASDNLKSRALVGTAPEQ
                     LVARVRDAGLAFDAGTRTGTTLHLLGALRSFGKVGVTCVADSPPAADELYREVAHLLT
                     GRGPGPRGQGLARR"
     gene            758..1363
                     /gene="lexA"
                     /locus_tag="VEP73_09875"
     CDS             758..1363
                     /gene="lexA"
                     /locus_tag="VEP73_09875"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYT26776.1"
                     /translation="MYETLAEEHQRILAVVREARAEGHVPSYREIGAAVGIASTATVH
                     RHLKALETMGFLRLGEGQRRAIELARESPPVSVPLVGKIAAGTPITAEQQIDERYCLP
                     RELLGAGRLFMLQVAGHSMTGAGVLDGDYVVVREQPTAENGEMVAAQLDNAEPEATVK
                     YWSVEGERRLLLPASAGYEPIDGSRARILGKVVAVVRGRVR"
     gene            1465..>2186
                     /locus_tag="VEP73_09880"
     CDS             1465..>2186
                     /locus_tag="VEP73_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYT26777.1"
                     /translation="MGGHPVARLDGPGGTQVPAGVIAAVARHYETANANAHGLFAASV
                     ETDAVIAGAREAMAALLGAAGPSTISFGQNMTTLNFSLAHAVARTVRPGDEVVVTDLD
                     HDANVAPWGVLAERGAVLRHVPVWPDATLDMEAFRALVGERTRLVAVGYASNAVGTVN
                     DLAAVRAWTREVGALLVVDAVHWVPHGVVDVAELDPDFLLCSAYKFFGPHVGVLYSRP
                     GALDALATDKVRPQLDRAPERV"
BASE COUNT          264 a          819 c          816 g          287 t
ORIGIN      
        1 cgaggcgggc ggggggatcg tcgaggagct gatccgggcc cccggcgtgg tctcgccgag
       61 cgtccagctc cggatcgccc cgggcggcga ggtcgagctc gtctcgaccc acagccagct
      121 cctcggcggc cccggcggcc aggtctacct cggctgccgc ttcccggccg acaacgccta
      181 ccggctcgac atccagcgct gggcgctgcg ggtcggggag ctgctggcgg ccaggggcgt
      241 catcggctgg ctcggcgtcg acttcatcgt cgtgccgtcg ccgtccggcg tcgagaccta
      301 cctgagcgag atcaacctgc gcatcggcgg taccacccat ccgctctgga tggcgctcct
      361 ggcctcggag gggcgctacg agccggccag cggccggctc ctggcggccg gcagggccaa
      421 gtcctacgtc gcgagcgaca acctgaagtc gcgcgccctg gtggggacgg cgccggagca
      481 gctggtggcc agggtgcgcg acgccggcct cgccttcgac gcggggaccc ggaccggtac
      541 gaccctgcac ctgctcggcg cgctgcgctc cttcggcaag gtgggcgtca cctgcgtcgc
      601 cgactcgccg ccggccgccg acgagctgta ccgcgaggtg gcccacctgc tcaccggccg
      661 aggtcctggg ccgcgcgggc aaggtctggc caggcgctga ccggcatgcg atcctcacct
      721 cgtccgggat cgggctcgag gcaccaggcg gtcgacgatg tacgagacgc tggccgagga
      781 gcaccagcgc atcctcgcgg tggtccgcga ggcccgcgcc gagggccacg tgccctccta
      841 ccgggagatc ggcgccgcgg tggggatcgc gtccacggcc accgtgcacc ggcacctcaa
      901 ggcgctcgag acgatggggt tcctccggct cggggagggc cagcgccgtg ccatcgagct
      961 ggccagggag tcgcccccgg tgtcggtccc gctcgtcggc aagatcgcgg cgggcacgcc
     1021 gatcaccgcc gagcagcaga tcgacgagcg ctactgcctg ccccgcgagc tcctcggcgc
     1081 cggccggctc ttcatgctcc aggtggccgg gcactccatg accggcgccg gcgtgctcga
     1141 cggtgactac gtcgtggtgc gcgagcagcc cacggccgag aacggcgaga tggtggcagc
     1201 ccagctcgac aacgccgagc ccgaggcgac cgtcaagtac tggtcggtcg agggggagcg
     1261 gcggctcctc ctgcccgcca gcgccgggta cgagcccatc gacggctccc gggcgaggat
     1321 cctcggcaag gtcgtggccg tcgtgcgcgg ccgggtccgg tgagccgccg ggagtacgac
     1381 gaggaccgcg cccgctgccg gctgagctgg cccgtgcccg acctcagccg ttgccgcggc
     1441 cagttcccat cgctcggtcg ggtggtgggc ggccatccgg tcgcccgcct cgacggcccg
     1501 ggcggcacgc aggtgccggc cggcgtgatc gcggccgtcg cccgtcacta cgagaccgcc
     1561 aacgccaacg cccacgggct gttcgccgcc agcgtcgaga ccgacgcggt gatcgcgggc
     1621 gcccgggagg cgatggcggc gctcctcggc gcggccgggc cgtcgacgat ctcgttcggg
     1681 cagaacatga cgacgctgaa cttctccctc gcccacgcgg tcgcccgcac ggtgcggccg
     1741 ggcgacgagg tggtggtcac cgacctcgac cacgacgcca acgtcgcgcc ctggggggtg
     1801 ctcgccgagc gcggggcggt gctgcggcac gtcccggtgt ggcccgacgc caccctcgac
     1861 atggaggcgt tccgcgcgct cgtcggcgag cggacccggc tcgtcgcggt cgggtacgcc
     1921 tcgaacgcgg tcggcaccgt gaacgacctc gccgccgtcc gggcctggac ccgcgaggtg
     1981 ggcgccctgc tggtcgtcga cgcggtccac tgggtgcccc acggcgtggt cgacgtcgcc
     2041 gagctcgacc cggacttcct gctctgctcc gcctacaagt tcttcgggcc ccacgtgggc
     2101 gtgctgtact cccggccggg cgcgctcgac gccctggcca ccgacaaggt gcggccgcag
     2161 ctcgaccggg cgccggagcg ggtgga
//
LOCUS       DASPYY010000627         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2633548, whole genome shotgun sequence.
ACCESSION   DASPYY010000627 DASPYY010000000
VERSION     DASPYY010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633548"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..2718
                     /gene="pepN"
                     /locus_tag="VEP73_09885"
     CDS             148..2718
                     /gene="pepN"
                     /locus_tag="VEP73_09885"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HYT26778.1"
                     /translation="MGHGGSLTRAEAGARARLVSEVGYDVELDLAGGDPEAYRSTTTV
                     RFRCAEPGASTFLDLAARSVEGVRLNGREVPAGALDGDRIRLDGLDARNEVRVVATPS
                     YGRTGVGMHRFEDPVDGRVYVYNHFEPYAHRIFPCFDQPDLKAVFTLSVLAPPAWQVV
                     SNSRLDEEPAAAPGGAVRWSFAPTPPIPTYVTAVVAGPWHVVRDRSGAVELGLWCRQS
                     LARHLDPEEFLAVTRQGFRFFERAFARPYPFGAKYDQVLVPEFGGGAMEQAACVTFDE
                     EFVFRSKVTDAAREVRANVVLHELAHMWFGNLVTMRWWDDLWLNESFASFISVLAQVA
                     ATRFTDGWVTFAQTEKTWAYEQDQLPSTHPIATEVPDTDTVFTNFDGITYAKGAAVLR
                     QLVAWVGEEAFLRGVRAYFQRHEFGSTGLGDFLDALGDASARDLKSWSRAWLETAGVN
                     TLATVLEVTGSAEVTGPAPDRRIGSFAIIQTAPAGHPVLRPHRVTVGLYDRDGGTLAL
                     RKRLELDVTGPVTPVPELAGEPAPDLALLNDADLAYAKVRLDPGSLSVLTGAVGALRD
                     PLARAVGWGICWDMVRDAELATSDHLRMVLANAEAEDHVGVLADVLDRLGHAVDVFGD
                     PAARPAARASLAGLAQRVLGRAEPGSGRQLAWAKALVATAETEEQLRLLRALLDGAAS
                     VPGLAVDTDLRWAIVRSLAAGGAAGDELISGELRRDPTDDGRRHAAGARAARPLPEAK
                     AEAWAVVTGPAGPARPLQSAVELGFQQAGQRELLEPYAARFFAALDPLAETRDLESLL
                     AFARAFYPRLVAGDEVVAMTDAWLASHPDAGPVRRRLVEGSDRMRRALRARARDRATS
                     "
     gene            complement(2782..3201)
                     /gene="msrB"
                     /locus_tag="VEP73_09890"
     CDS             complement(2782..3201)
                     /gene="msrB"
                     /locus_tag="VEP73_09890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYT26779.1"
                     /translation="MSDTSGTGITRTEQEWREQLTPEQYEVLRRKGTEPPFTGRYTYS
                     KADGTYRCAACGAPLFSSATKFDSGTGWPSFTEPATADAVELRPDSSLFMRRTEVVCR
                     RCGSHLGHVFDDGPAPTGQRYCINSISLDLDEATPGP"
     gene            complement(3255..>3649)
                     /locus_tag="VEP73_09895"
     CDS             complement(3255..>3649)
                     /locus_tag="VEP73_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYT26780.1"
                     /translation="AAALRTGAGYVGMVGSRRRAAAVLEALRGRGVGDADLARVHSPA
                     GLDLGPSTQQEIAVAILAEVVRERHRRAAAPPAPAEQATDPVCGMAVAVPGARFTARH
                     AGRTWYFCGEHCLHAFRRDPGRFTAPRA"
BASE COUNT          421 a         1403 c         1350 g          475 t
ORIGIN      
        1 gatccggctg gccagaaccg actagtcacc cggtcggccg gcccccggag cgccgtgtct
       61 caccccggtg aggggatctc ctgggcgggg acatctccgg gctgcacgga ggcagccctg
      121 gctggtgcga cacgacggga gcggcggatg gggcacggcg gcagcctgac gcgcgccgag
      181 gcgggggcca gggcccggct cgtctccgag gtcggctacg acgtcgagct cgacctcgcg
      241 ggcggcgacc cggaggccta ccgcagcacg accacggtcc gcttccgctg cgccgagccg
      301 ggcgcctcga cgttcctcga cctcgccgcc cggtcggtcg agggcgtgcg gctgaacggc
      361 cgggaggtcc cggccggcgc gctcgacggc gaccggatcc gcctcgacgg cctggacgcc
      421 aggaacgagg tccgcgtcgt cgccaccccc tcctacggcc gcacgggtgt cggcatgcac
      481 cgcttcgagg atccggtcga cggccgcgtc tacgtctaca accatttcga gccgtacgcg
      541 caccgcatct tcccctgctt cgaccagccc gacctgaagg ccgtcttcac cctgtcggtg
      601 ctcgccccgc ccgcctggca ggtcgtctcc aacagccggc tcgacgagga gcccgccgcg
      661 gcccccggcg gggccgtccg ctggtccttc gccccgaccc cgccgatccc cacgtacgtg
      721 acggcggtcg tcgccgggcc gtggcacgtc gtccgcgacc ggagcggggc ggtcgagctc
      781 gggctgtggt gcaggcagtc gctcgcccgc catctcgacc ccgaggagtt cctcgcggtc
      841 accaggcagg gcttccgctt cttcgagcgg gccttcgcgc gaccctatcc gttcggcgcc
      901 aagtacgacc aggtgctggt gccggagttc ggcggcggcg ccatggagca ggccgcctgc
      961 gtcaccttcg acgaggagtt cgtcttccgt tccaaggtga ccgatgcggc cagggaggtc
     1021 cgggccaacg tcgtgctgca cgagctcgct cacatgtggt tcggcaacct cgtgaccatg
     1081 cggtggtggg acgacctgtg gctgaacgag agcttcgcct ccttcatctc ggtcctcgcc
     1141 caggtggccg ccacccgctt cacggacggc tgggtcacct tcgcccagac cgagaagacc
     1201 tgggcgtacg agcaggacca gcttccctcg acgcacccga tcgcgaccga ggtccccgac
     1261 accgacaccg tcttcacgaa cttcgacggg atcacctacg cgaagggcgc cgctgtcctg
     1321 cgccagctcg tcgcgtgggt cggggaggag gcgttcctca ggggcgtgcg cgcctacttc
     1381 cagaggcacg agttcggcag caccggcctc ggcgacttcc tcgacgcgct gggggacgcc
     1441 tcggcccgcg acctcaagtc ctggtcgcgg gcctggctgg agactgccgg ggtgaacacc
     1501 ctcgccacgg tgctcgaggt gaccgggtcg gcagaggtga ccggtccggc gccggaccgg
     1561 aggatcggct cgttcgcgat catccagacc gcgccggccg ggcaccccgt cctccggccg
     1621 caccgggtca ccgtcgggct ctacgaccgc gacggcggca cgctcgcgct gcgcaagcgg
     1681 ctcgagctgg acgtcaccgg accggtcacg ccagtgcccg agctcgccgg cgagcccgcc
     1741 cccgacctcg cgctgctgaa cgacgccgac ctcgcgtacg cgaaggtccg cctcgacccc
     1801 gggtcgctgt cggtgctgac gggggccgtc ggcgccttgc gcgacccgct ggccagggcg
     1861 gtcggctggg ggatctgctg ggacatggtg cgcgacgcgg agctggcgac cagcgaccac
     1921 ctccggatgg tgctggccaa tgccgaggcc gaggaccacg tcggcgtgct cgccgacgtc
     1981 ctcgaccggc tcggccacgc cgtcgacgtc ttcggcgacc cggcggcgcg gccggccgcc
     2041 cgggcgagcc tggccggtct ggcgcagcgc gtgctcgggc gggccgagcc gggcagcggc
     2101 cgccagctgg cctgggccaa ggcgctggtc gccacggccg agaccgagga gcagctccgg
     2161 ctgctccgcg ccctgctcga cggggccgcg agcgtccccg gcctggccgt ggacaccgac
     2221 ctgcgctggg cgatcgtccg gtcgctcgcg gccggcggcg cggcgggcga cgagctgatc
     2281 tccggcgagc tgcgccgcga cccgaccgac gacggcaggc ggcacgccgc gggggcccgc
     2341 gccgcccgcc ccctgcccga ggccaaggcc gaggcctggg cggtggtcac cggccccgcc
     2401 ggcccggccc ggcccctcca gagcgcggtc gagctcggct tccagcaggc cgggcagcgg
     2461 gagctgctcg agccctacgc ggcccggttc ttcgccgcgc tcgacccgct cgccgagacg
     2521 cgcgacctgg agtccctgct ggccttcgcc cgcgccttct acccccgtct ggtggccggg
     2581 gacgaggtcg tcgcgatgac cgacgcctgg ctcgcctcgc acccggacgc cggcccggtc
     2641 cgccgccgcc tcgtcgaggg ctccgaccgc atgcggcgcg ccctccgcgc ccgcgcgagg
     2701 gaccgggcga cctcctgaac ccccgccgcc gccggtcccg cgcccgcggt gcggccggcc
     2761 ccgccccggg cggcccgtcg gctacggccc gggcgtggcc tcgtcgaggt cgagcgagat
     2821 cgagttgatg cagtagcgct ggccggtcgg ggcggggccg tcgtcgaaga cgtgcccgag
     2881 gtgcgacccg cagcggcggc agacgacctc ggtgcggcgc atgaagaggc tcgagtccgg
     2941 gcggagctcg acggcgtcgg cggtcgccgg ctcggtgaag ctcggccagc cggtgccaga
     3001 gtcgaacttc gtggcggagc tgaacagcgg cgcgccgcag gcggcgcacc ggtaggtccc
     3061 gtccgccttg gagtaggtgt acctgccggt gaacggcggc tcggtgccct tgcgccgcag
     3121 cacctcgtac tgctcgggtg tgagctgctc gcgccactcc tgctcggtcc tggtgatccc
     3181 ggtcccgctg gtgtcgctca tcggtacctc cggagtcctc gtggtcggtg accagggtac
     3241 ggctcccgcg ggcctcaggc ccgcggagcg gtgaagcggc cggggtcgcg ccggaacgcg
     3301 tgcaggcagt gctcgccgca gaagtaccag gtgcggccgg cgtgccgggc cgtgaagcgc
     3361 gcgcccggga cggccacggc catgccgcag acggggtcgg tggcctgctc ggccggcgcg
     3421 ggcggcgccg ccgcccgccg gtgccgctcc cgcaccacct cggcgaggat cgccaccgcg
     3481 atctcctgct gggtcgacgg gccgaggtcg agcccggccg ggctgtgcac ccgggccagg
     3541 tcggcgtcgc ccacgccgcg cccgcggagc gcctccagca cggccgccgc ccgcctgcgg
     3601 ctgccgacca tgccgacgta gccggctccg gtgcggagcg cggcggcca
//
LOCUS       DASPYY010000628         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_807728, whole genome shotgun sequence.
ACCESSION   DASPYY010000628 DASPYY010000000
VERSION     DASPYY010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807728"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VEP73_09900"
     CDS             <1..621
                     /locus_tag="VEP73_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26781.1"
                     /translation="RPARRGPRRLAALLSLVVVVLLGLLGWWAVATGRLLGPGPAAPA
                     PAGTAPPAGQGWTPYAHEGGAFTVRHPPGWSVDPDAAERWVRFLDPAGAGRFFKVQVD
                     EDRKDPLRAWEDTEKDFRADLRNHPGYRRVALERTAFELPGAEPARAALWEFTYDRNG
                     RPTHAWDLSVVTASRRYSVLFQTDARVWESSRGLLDAFLQGFQPAA"
     gene            710..2320
                     /locus_tag="VEP73_09905"
     CDS             710..2320
                     /locus_tag="VEP73_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYT26782.1"
                     /translation="MAHDEARRPDERRLTGSGIPVEVLYGPGDLDGFDPAAALGEPGR
                     DPFTRGIHPTMYRGRPWTMRQYAGFGSAAETNARFRYLLRHGQTGLSVAFDLPTQMGY
                     DSDHPMAAGEVGKVGVAIDSLADMRRLFEDIPLDQVSTSMTINAPAAVLLLLYQLVGE
                     EQGVDPAALTGTVQNDVLKEYIARGTYIYPPAPSLRITTDLFGYCAANLPRFNTISIS
                     GYHMAEAGATAVQEVAFTLANALAYVQAALDAGLSVDDFAPRLAFFFVARTALFEEVA
                     KFRAARRIWADLMASRLGAENPKSRMLRFHTQTAGVELTAQQPLNNVVRVTLQGLAAV
                     LGGTQSLHTNSFDEALALPSETAARLALRTQQLLANETGVTDTVDPLAGSYFVERLTD
                     EVEARIRDLLARIDELGGAVPAIERGFQKEEIERSAYAHALAVESGEKVVVGVNRYTE
                     GEEEPVQLLRLDPAIAEGQAKSLAAVRAGRDNDATRAALAALRDAAEGTGNLLVPMRE
                     ALRGMATVGEVCDALREVFGTYRPPETF"
     gene            2451..2627
                     /locus_tag="VEP73_09910"
     CDS             2451..2627
                     /locus_tag="VEP73_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26783.1"
                     /translation="MSNSVQALFYFLALVCFVGAAFAGVSRSSFVSRVNLIALGLAFW
                     VFVPFWNAVSKITS"
     gene            2726..>3057
                     /locus_tag="VEP73_09915"
     CDS             2726..>3057
                     /locus_tag="VEP73_09915"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYT26784.1"
                     /translation="MVASTHWIASAAGMAMLERGGNAFDAAVAAGLVLQVVEPHQNGP
                     GGEAPILLWDARRRRPLVVAGQGPAPAAATIERFRDLGLDLVPGTGLLAACVPGAFDA
                     WMRLVRDYG"
BASE COUNT          384 a         1142 c         1108 g          423 t
ORIGIN      
        1 cggccggccc ggcgcggccc gcgccgcctc gcggccctcc tcagcctcgt cgtcgtggtc
       61 ctgctcgggc tgctcggctg gtgggcggtc gccaccgggc ggctcctcgg gccgggcccg
      121 gccgcaccgg caccggcggg cacggcccct ccggcgggcc agggctggac accgtatgcc
      181 cacgagggcg gcgcgttcac ggtgcgccac ccgcctggct ggtccgtcga cccggacgcc
      241 gcggagcggt gggtgcggtt cttggacccg gccggtgcag gccggttctt caaggtccag
      301 gtcgacgagg accgcaagga cccgctgcgc gcctgggagg acaccgagaa ggacttccgg
      361 gctgacctga ggaaccaccc cggctaccgg cgggtcgccc tcgagcggac agcgttcgag
      421 ctgcctggcg ccgagcccgc ccgcgccgcg ctgtgggagt tcacctacga ccggaacggg
      481 cgccccaccc acgcgtggga cctgtcggtg gtgaccgcga gccgccgcta ctcggtgctg
      541 ttccagaccg acgcgcgcgt ctgggagagc tcgcgcggcc tgctcgacgc gttcctccag
      601 gggttccagc cggccgcgta ggcgtcgcgg gcggccgggg cggggcatag gctggtcccc
      661 gccgcctcgg gagcggaccg gggcggcgcg ccgaggtctg ggaggcagca tggcgcacga
      721 cgaagcgcgg cgacccgacg agcggcgact gaccgggtcc ggcatcccgg tcgaggtcct
      781 ctacggacca ggcgacctgg acggcttcga cccggccgcc gcgctcggcg agccgggacg
      841 ggaccccttc acccgcggca tccacccgac gatgtaccgg ggccgtccgt ggacgatgcg
      901 gcagtacgcg ggcttcgggt cggccgcaga gaccaacgcg cgcttccgct acctgctgcg
      961 ccacggccag accggcctgt cggtcgcctt cgacctgccg acccagatgg ggtacgactc
     1021 cgaccacccg atggccgccg gcgaggtggg caaggtcggg gtggcgatcg actccctggc
     1081 ggacatgcgc cgcctgttcg aggacatccc gctcgaccag gtgtcgacgt cgatgacgat
     1141 caacgcgccg gcggccgtcc tgctgctcct gtaccagctc gtgggggagg agcagggggt
     1201 cgacccggcg gcgctcacgg gcacggtcca gaacgacgtg ctcaaggagt acatcgcccg
     1261 ggggacctac atctacccgc cggccccgag cctgcggatc acgacggacc tgttcggcta
     1321 ctgcgccgcc aacctgcccc gcttcaacac gatctcgatc tccggctacc acatggccga
     1381 ggcgggggcc accgcggtcc aggaggtcgc cttcacgctc gccaacgccc tcgcctacgt
     1441 gcaggccgcc ctggacgccg gcctgtccgt ggacgacttc gcgccgcggc tcgcgttctt
     1501 cttcgtcgcc cggacggcgt tgttcgagga ggtcgccaag ttccgcgcgg ccaggcgcat
     1561 ctgggccgac ctgatggcga gccgcctcgg ggccgagaac cccaagtccc gcatgctgcg
     1621 gttccacacc cagaccgccg gggtcgagct caccgcccag cagcccctga acaacgtggt
     1681 cagggtcacg ctgcaggggc tcgcggcggt gctcgggggt acccagtcgc tgcacacgaa
     1741 ctccttcgac gaggcgctcg ccctgccgag cgagaccgcc gcccgcctgg ccctgcgcac
     1801 ccagcagctg ctggccaacg agaccggggt gaccgacacg gtcgacccgc tggccggctc
     1861 gtacttcgtg gagcgcctga ccgacgaggt cgaggcgcgg atccgcgacc tcctcgccag
     1921 gatcgacgag ctcggcggcg cggtccccgc catcgagcgg ggcttccaga aggaagagat
     1981 cgagcgctcg gcctacgccc acgccctggc ggtggagtcg ggcgagaagg tggtcgtcgg
     2041 cgtgaaccgg tacaccgagg gcgaggagga gccggtccag ctgctgcgcc tcgacccggc
     2101 catcgccgag gggcaggcca agtcgctcgc ggcggtgcgg gccggccgcg acaacgacgc
     2161 cacccgcgct gccctcgccg ccctcaggga cgccgccgag gggaccggca acctgctcgt
     2221 gcccatgcgc gaggcgctgc gcggcatggc gaccgtcggc gaggtctgcg acgccctgcg
     2281 cgaggtgttc ggcacctatc ggccgcccga gaccttctga cgtctggagc tctctggctg
     2341 ggtctgatcc gttcaattgc ggacggatcg cacgccgaat tggtgatttg ctcctggggt
     2401 ccggcgcgac cgggacgccg gagtcccctt ccacacggag gaatctcgcg atgtcgaaca
     2461 gcgtgcaggc gctcttctac ttcctcgccc tcgtctgctt cgtcggggcc gcgttcgccg
     2521 gcgtcagccg gtcctcgttc gtgagccggg tcaacctgat cgccctcggg ctcgcgttct
     2581 gggtcttcgt gcccttctgg aacgcggtgt cgaagatcac ctcctgagcg ccgcctcctc
     2641 gcacgggcgc cggccctggc caggccggac gggtagactg gcgggcgtgt tcacgacccg
     2701 cccggagctc tccggcaccc acggcatggt ggcgtccacc cactggatcg cgtccgcggc
     2761 cggcatggcc atgctcgagc ggggcggcaa cgccttcgac gccgcggtcg cggccggcct
     2821 cgtgctgcag gtggtcgagc cccaccagaa cggcccgggc ggggaggccc cgatcctgct
     2881 ctgggacgcc cgccggcgcc ggccgctggt cgtcgccggg cagggcccgg cgcccgcggc
     2941 cgcgacgatc gagcgcttcc gcgacctcgg cctggacctg gtgcccggca ccggcctgct
     3001 ggcggcgtgc gtgcccggcg cgttcgacgc ctggatgcgg ctcgtgcgcg actatgg
//
LOCUS       DASPYY010000629         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_103952, whole genome shotgun sequence.
ACCESSION   DASPYY010000629 DASPYY010000000
VERSION     DASPYY010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103952"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VEP73_09920"
     CDS             <1..1288
                     /locus_tag="VEP73_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYT26785.1"
                     /translation="AERLDPVYRVVEESMRAQPSLSRQTLSRRTLLLPMVLALVLLGG
                     PLAAPAKAGDPAAVDVSFALLATATNPVAITFSPDATDRVFVVEQAGQVRVLDRWRHL
                     LPTPYLDIRSRVLSGGERGLLSVAFHPRFSQNGRFFAAYTDAQGDLRVSRFQADPASD
                     TAPASSERVLLDVAHRDFANHNGGQVAFGPDGYLYVSTGDGGGGGDPDGNAMNRTRLL
                     GKVLRIDVDHAEGGRPYAIPPTNPYARNHSLRPEIWHYGLRNAWRFSFDRFGTMWIAD
                     VGQAAREEVDVVLPRVGNRNFGWDCREGMLDTAAAYGGAYCESRAPTFTWPRLEYDHG
                     GGRCGIHGGGRCGIIGGYRYRGRAYRRLLGDAYLYGDLCSGQVWALAHLATGEWRNAQ
                     VGRFPGTLSSFGESRSGEVYAADLGGGVYRVMARST"
     gene            complement(1683..2309)
                     /locus_tag="VEP73_09925"
     CDS             complement(1683..2309)
                     /locus_tag="VEP73_09925"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HYT26786.1"
                     /translation="MGGPPGIRTRCAARSGDTASYWTPALYRNGVKVDPRGTGTRQQV
                     YYDRSNLLPGTRVETIPADLRVKAGVGTARSLAENPGVGREIYWGCSDNSTSGKSTVP
                     PAHCPTGIITLHLGFPNCWDGGLTHVDDSAHLAYPKRGRCVAPFAHVLPRVILRWEYP
                     VGPTTGLITLSSGSLATVHGDFWNTWQQPALTRLVHGCINAGRDCGKR"
BASE COUNT          336 a          886 c          906 g          334 t
ORIGIN      
        1 agccgaacgt cttgatccgg tctatcgagt cgtggaggag agcatgcgcg cacagccgtc
       61 cctcagccga cagactctca gccgacgaac cctcctgctc cccatggtgc tggcgctcgt
      121 cctcctcggc gggccgctgg ccgccccggc caaggccggc gacccggcgg cggtcgatgt
      181 gtccttcgcg ctgctcgcga ccgcgacgaa ccccgtcgcg atcacattct cacccgacgc
      241 gaccgaccgg gtgttcgtgg tcgagcaggc cgggcaggtc cgggtcctcg accgctggcg
      301 ccacctcctc cccaccccgt acctcgacat ccgctcacgg gtgctgagcg gcggcgagcg
      361 cggcctcctc tcggtcgcct tccacccccg cttcagccag aacggccggt tcttcgccgc
      421 ctacaccgac gcccagggcg acctgcgcgt ctcccgcttc caagccgacc ccgcgagcga
      481 caccgcgccc gcctcgtccg agcgcgtgct gctcgacgtc gcgcaccgcg acttcgccaa
      541 ccacaacggg ggacaggtcg cgttcgggcc cgacggctac ctctacgtgt cgaccgggga
      601 cggcgggggt ggcggcgacc cggacggcaa cgccatgaac cgcacccggc tgctgggcaa
      661 ggtcctgcgc atcgacgtcg accatgccga gggcggccgg ccgtacgcca tcccgcccac
      721 caacccgtac gcgcggaacc acagcctgcg ccccgagatc tggcactacg gcctgcggaa
      781 cgcctggcgg ttcagcttcg accgcttcgg caccatgtgg atcgccgacg tcggccaggc
      841 cgcccgcgag gaggtcgacg tcgtcctccc gcgggtcggc aaccggaact tcgggtggga
      901 ctgccgggag gggatgctcg acacggcggc ggcctacggc ggggcgtact gcgagagccg
      961 cgccccgacg ttcacctggc cgcgcctcga gtacgatcac ggcgggggcc ggtgcggcat
     1021 ccacggcggg ggccggtgcg gcatcatcgg cgggtaccgg taccgcggcc gggcctaccg
     1081 ccgcctcctc ggcgacgcct acctgtacgg cgacctgtgc agcggccagg tctgggcgct
     1141 cgcccacctc gccaccggcg agtggaggaa cgcccaggtc gggaggttcc cgggcacgct
     1201 cagctccttc ggcgagagcc gctcgggcga ggtgtacgcg gccgacctgg gcggcggggt
     1261 ctaccgcgtg atggcccgct ccacctgagc ggcccgctcc agcccggtca caggcgcaca
     1321 aggtcacagg aaggtctcga tcaggtcgca gacggcaccg acccgtatct gtccgcaccc
     1381 ctgctgcgcc tatgtacgtg aaccgccgag cacggaagca cggagggatg aacatgaaca
     1441 ggctgcttcg gtccgtcagc ccccgttccc tgcgctaccg cggtcggcac ctgcggtacc
     1501 gcggccggca cgtcaggccg cgctgagcca gcaccaccgc cgacggttcc gggccggtcc
     1561 cccgggccgt cggcgctcac cacggtcgcg ccgggggcgt tgaggagggg ccgccccggg
     1621 cgcgaccgtg gtcgttcgtg aatgggggac ggccggcggc cgtcccccat tcacgtgcgg
     1681 tgtcagcgct tcccgcagtc cctgcccgcg ttgatgcagc cgtgcaccag gcgagtgagg
     1741 gcgggctgct gccaggtgtt ccagaagtcg ccgtggaccg tggcgaggga tccggacgag
     1801 agggtgatga gcccggtggt cgggccgacc gggtactccc agcgcaggat gaccctgggg
     1861 agcacgtgcg cgaacggggc gacacaccgc ccccgcttcg ggtaggcgag gtgggcggag
     1921 tcgtcgacgt gggtcaggcc cccgtcccag cagttgggga agccgaggtg cagggtgatg
     1981 atgccggtcg ggcagtgcgc gggcgggacg gtcgacttgc cgctggtcga gttgtccgag
     2041 cagccccagt agatctcgcg gccgaccccg gggttctcgg ccaggctgcg ggccgtgccg
     2101 acccctgcct tgactcgcag gtcggcgggg atggtttcga ctctcgtgcc gggcaggagg
     2161 ttggagcggt cgtagtagac ctgctggcgg gtgccggttc ctctcgggtc gaccttgacc
     2221 ccgttgcggt acagggcggg cgtccagtag ctcgccgtgt cgcccgacct cgccgcgcag
     2281 cgggtccgga tcccgggagg gccgcccacc gagtcggagg cacgccggtc ggaggcacgg
     2341 cttggcgtgc cgagcgggac agagcgaggc gtgccgagcg ggacagagcg aggcgtgccg
     2401 agcgggacag agcgaggcgt gccgagcggg acagagcgag gcgtgccgga cgggagaggg
     2461 ag
//
LOCUS       DASPYY010000630         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1231202, whole genome shotgun sequence.
ACCESSION   DASPYY010000630 DASPYY010000000
VERSION     DASPYY010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231202"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="cpaB"
                     /locus_tag="VEP73_09930"
     CDS             <1..444
                     /gene="cpaB"
                     /locus_tag="VEP73_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HYT26787.1"
                     /translation="ILANEQLVDSRWVSQEEVEGQNLLTIKPGFQAVSIQVEASKQVS
                     GFVTVNNHVNVYVTIDKAGGPVTQLLLADVKVLAVGATAQGTTAAQPPGRNGNLSTLT
                     LEVRDQQVDKLVFAADNGRLYLTLVPPTGGPTPQGAPTGIGNLFN"
     gene            479..>1546
                     /locus_tag="VEP73_09935"
     CDS             479..>1546
                     /locus_tag="VEP73_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HYT26788.1"
                     /translation="MAVIIAESEDGYATWLASTLAGLTDRVIRTVSPDEAMAALAAEG
                     RDVLGMIIGPDLDDREALGLAGMTQQGAPDVSVLLLRYHDSSDLFRAALRFGVKDVIT
                     TPGDEDTVRAAAARALEIARALRGRLAGGVPAGTEGAEPPGKVVTVFSSKGGCGKTFL
                     ATNLAFALAGTGAEVALVDLDLHFGDVAIMLQLFPAHTIQDAAETQGLDTMALKSLLT
                     HHRQGIWALLAPTEPTVADTITPQAVAAIVKLLRENFDYVVIDTPAAFSDQVLAAFDE
                     SDAIAMLATLDVPSIKNLKLALQTMELLHFPRNRLRVVVNRADSKVGLRLADVEKILG
                     APVDATIPSSRSVPLSVNKGHP"
BASE COUNT          240 a          592 c          509 g          205 t
ORIGIN      
        1 atcctcgcca acgagcagct cgtcgactcg aggtgggtga gccaggagga ggtcgagggg
       61 cagaacctgc tcaccatcaa gccgggcttc caggccgtct cgatccaggt cgaggccagc
      121 aagcaggtct ccggcttcgt caccgtcaac aaccacgtga acgtctacgt gaccatcgac
      181 aaggccggtg gcccggtgac ccagctcctg ctcgcggacg tcaaggtgct cgcggtcggc
      241 gcgaccgccc aggggaccac ggcggcccag ccgcctggcc gcaacggcaa cctctcgacc
      301 ctcaccctcg aggtgcgcga ccagcaggtc gacaagctcg tcttcgcggc cgacaacggc
      361 cggctctacc tgacgctggt cccgccgacc ggcggcccca ccccccaggg cgcgccgacg
      421 ggcatcggca acctgttcaa ctagtcgagt ggacgcaccg agcaccggga gcaccgccat
      481 ggccgtgatc atcgccgaat cagaggacgg ctacgcgacc tggctggcga gcaccctcgc
      541 cgggctgacc gaccgcgtca tccgcaccgt cagcccggac gaggccatgg cggcgctcgc
      601 cgccgagggc cgcgacgtgc tcggcatgat catcgggccg gacctcgacg accgggaggc
      661 cctcggcctc gccggcatga cgcagcaggg cgcgcccgac gtctccgtcc tgctgctgcg
      721 ctaccacgac tcgtccgacc tgttccgcgc cgccctgcgg ttcggggtca aggacgtgat
      781 caccaccccg ggcgacgagg acacggtgcg cgcggccgcg gccagggccc tcgagatcgc
      841 gcgggcgctg cgcggccgcc tggccggcgg ggtgcccgcc ggcaccgagg gcgccgagcc
      901 gccgggcaag gtcgtcacgg tgttcagctc caagggcggc tgcggcaaga cgttcctcgc
      961 gacgaacctc gccttcgccc tggcgggcac cggcgccgag gtggccctcg tcgacctcga
     1021 cctgcacttc ggcgacgtcg ccatcatgct gcagctcttc ccggcccaca ccatccagga
     1081 cgcggccgag acccagggcc tggacacgat ggcgctgaag tcgctgctga cccatcaccg
     1141 gcaggggatc tgggcgctgc tcgccccgac cgagccgacg gtggccgaca cgatcacccc
     1201 ccaggcggtc gccgccatcg tcaagctgct gcgggagaat ttcgactacg tcgtcatcga
     1261 caccccggcg gccttctccg accaggtgct cgcggccttc gacgagtcgg acgcgatcgc
     1321 gatgctcgcg accctggacg tgccgagcat caagaacctc aagctcgcct tgcagaccat
     1381 ggagctgctc cacttccccc gcaaccggct ccgggtggtg gtcaaccggg ccgactcgaa
     1441 ggtcggcctg cgcctggcgg acgtggagaa gatcctcggc gcgccggtcg acgcgaccat
     1501 cccctcctcg cgctcggtgc cgctgtcggt caacaagggc cacccc
//
LOCUS       DASPYY010000631         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1370221, whole genome shotgun sequence.
ACCESSION   DASPYY010000631 DASPYY010000000
VERSION     DASPYY010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370221"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VEP73_09940"
     CDS             <1..829
                     /locus_tag="VEP73_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYT26789.1"
                     /translation="AGPANVAQLAMDTGVGATLSSTEHRPHLDKLPSLALGASEVSTL
                     QLASAYGTWANRGVHVQPYVIEDITDLAGEPIRIADVHGQPAERHQVRGRRVVDENVA
                     DTVNMALSGVIKDGTGRNADIARPAAGKTGTTNDFTDARFVGYTPNLVASVWMGYDNP
                     HRKLENVHGQRAVSGGSFPALIWHDFMTLSLRGTQPLAFHPPTFDGQALNSTTTVAPA
                     TVTLPPTTTEPSSSVPPSSRSHGPKTTWPASSLPPSTTLFPETTVDRQRPPSSTNGG"
     gene            834..>2218
                     /locus_tag="VEP73_09945"
     CDS             834..>2218
                     /locus_tag="VEP73_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26790.1"
                     /translation="MRAATAERPAAGVRWPRLAGERAALLVVGLWVVTRAVLLVVSLN
                     PRLYSAALFGDVRLYGAKVERMFQGEVPYRDVAIEYPPGSVPFTILPGLAVGTGADYR
                     LAFAVEMLAVDAVGLWAASRLARLADAGRRRIPLAYVLAMGAIGPLLVLRFDLVPTVC
                     VLLAAALAADGRAGPAAAALGYGAAAKLFPGVLLPLLVLGLVPAEGWWRSLRRTVPPF
                     LAGFALTVVPALALSVKGTLGAVLYHVRRGVQIESLWATLIGLAHVAGGLPAHTVSGF
                     GAYDLDSSVSGAAKALSSVATLAALLAAAWLVWRRSSRSGGLGPADWAGALAIGVFAF
                     VLPTRVLSPQYLVWLAAPMAALADRREGRRALWVLVAAIVVSQVIFPFRYTQLRHLEV
                     FDVGLLAVRNLLLVVACALTVRAFGRGPGAAPVPAGTAGEPAATTVGNGLPSRAAYGP
                     APPSRRLRRRTP"
BASE COUNT          237 a          916 c          755 g          310 t
ORIGIN      
        1 ggccgggccc gcgaacgtcg cccagctcgc gatggacacc ggggtcggcg ccaccctgtc
       61 ctcgaccgag caccggccgc acctggacaa gctgccatcg ctcgccctcg gggccagcga
      121 ggtcagcacg ctgcagctcg cctccgccta cgggacctgg gccaaccggg gcgtccacgt
      181 ccagccctac gtgatcgagg acatcaccga cctcgccggc gagcccatcc ggatcgccga
      241 cgtccacggc cagccggccg agcggcacca ggtccggggg cgccgggtgg tggacgagaa
      301 cgtggccgac accgtcaaca tggccctctc cggcgtcatc aaggacggca ccggcaggaa
      361 cgccgacatc gcccggccgg cggccggcaa gacggggacg accaacgact tcaccgacgc
      421 ccgcttcgtc ggctacacgc cgaacctcgt cgcgtcggtc tggatgggct acgacaaccc
      481 ccaccgcaag ctcgagaacg tccacggcca gcgcgccgtg tccggcgggt ccttcccggc
      541 gctgatctgg cacgacttca tgacgctctc gctcaggggc acccagcccc tggcgttcca
      601 cccgccgacg ttcgacggcc aggcgctgaa ctcgaccacc accgtggcgc ccgcgaccgt
      661 cacgctgccg cccaccacga ccgagccctc gtcgagcgta ccgccgagca gccggtcgca
      721 cgggcccaag acgacctggc ccgcctccag cttgccgccg tcgaccacgc tgttccccga
      781 gaccacggtc gaccggcagc gaccgccctc gtccacgaac ggcggctagg gccgtgcgcg
      841 ccgccaccgc cgagcggccc gccgccgggg tgcgctggcc gcgcctggcg ggcgagcgcg
      901 ccgcgctgct cgtcgtcggg ctctgggtcg tcacgcgcgc cgtgctgctg gtggtctcgc
      961 tcaacccgag gctgtactcg gcggcgctgt tcggcgacgt ccgcctctac ggcgccaagg
     1021 tcgagcggat gttccagggt gaggtcccct accgggacgt cgccatcgag tacccgcctg
     1081 gcagcgtgcc gttcacgatc ctccccggcc tcgccgtcgg gaccggcgcc gactaccggc
     1141 tcgccttcgc cgtcgagatg ctcgccgtcg acgcggtcgg cctgtgggcg gccagccggc
     1201 tggcccggct cgctgacgcc ggtcgccgcc gcatccccct cgcctacgtc ctggccatgg
     1261 gcgccatcgg accgctgctc gtgctccgct tcgacctcgt gcccacggtc tgcgtcctgc
     1321 tcgccgccgc gctggccgcc gacggccggg ccgggccggc cgccgcggcg ctcggctacg
     1381 gcgccgccgc caagctcttc cccggggtgc tgctgcccct gctcgtcctc ggcctggtgc
     1441 cggcggaggg ctggtggcgg tccctccgcc gcacagtgcc gcccttcctt gccgggttcg
     1501 cgctcaccgt ggtgcccgcc ctggccctgt cggtcaaggg caccctcggg gcggtgctgt
     1561 accacgtgcg gcgcggggtg cagattgaga gcctctgggc caccttgatc ggcctggccc
     1621 acgtcgccgg cggcctcccc gcccacacgg tgtccggctt cggcgcctac gacctcgact
     1681 cgagcgtctc cggcgcggcc aaggccctgt cgagcgtcgc caccctggcc gccctcctcg
     1741 cggcggcctg gctggtgtgg cggcggtcga gccggtcggg cggcctcggc ccggccgact
     1801 gggcgggcgc gctcgccatc ggcgtcttcg cgttcgtgct gccgaccagg gtgctgtccc
     1861 cccagtacct ggtctggctc gccgccccca tggccgctct cgccgaccgc cgggagggcc
     1921 ggcgcgccct ctgggtgctc gtggcggcga tcgtggtcag ccaggtgatc ttcccgttcc
     1981 gctacaccca gctccggcac ctcgaggtgt tcgacgtcgg cctgctggcc gtgcgcaacc
     2041 tcctcctcgt ggtcgcctgc gccctcaccg tccgggcctt cggccgcggc cccggcgcgg
     2101 ctccggtccc cgccgggacc gccggggagc cagccgccac cacggtcggg aacgggctcc
     2161 ccagccgggc ggcctacggg cctgcgcccc cgtccaggcg cttgcgcagg cggacccc
//
LOCUS       DASPYY010000632         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1650730, whole genome shotgun sequence.
ACCESSION   DASPYY010000632 DASPYY010000000
VERSION     DASPYY010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650730"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VEP73_09950"
     CDS             <1..522
                     /locus_tag="VEP73_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYT26791.1"
                     /translation="HGDARSIKYNHGSLYMVVQQLAKAGFVVAQETTRDGGRPERTVY
                     ALTDAGRHEQRDWLRELVEEPQHEYPQFGAALSLIAALPPGEAVELLRRRLDRLAEQR
                     AEIRGLIDRTLATGVHPLFLVEEDYRLVLLDAESVFVERFVEQVNRPETGWGRPWGEF
                     HGEPANTDERQPS"
     gene            519..1733
                     /locus_tag="VEP73_09955"
     CDS             519..1733
                     /locus_tag="VEP73_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT26792.1"
                     /translation="MTHTRTALVIGGGIAGPVAAMALRRAGIQATVYEAHPSGAESVG
                     LFLTLASNGIDALRTIDADRPALAAGFPTPGITLRSTTGKHLGAARTGLSLRDGTTSH
                     TLKRADLYKAVHDEAASRGIVVEHGKRLIDVEQTGDGVRALFADGSEATGEVLIGADG
                     IHSVVRGLIDPNAPAPAYVGLVNLGGYARGVHVDAEPGSYTMIFGKRAFFGYALAPGG
                     EVWWFANVPRGDEPDRGEVEAITAAEWQRQLAELYAEDAGPAVRLVQASDTADVMKAS
                     PTHSIPHLPVWHTGRMIVIGDAAHAPTPSSGQGASLSVEDAVALAKCLRDLPHQEAFA
                     AYERMRRARVERIAKVAARVNSSKAAGPVARVFRDATLPVILKMTANSKQLNEQYRYH
                     IDWNTPISSVTA"
     gene            complement(1755..>2534)
                     /locus_tag="VEP73_09960"
     CDS             complement(1755..>2534)
                     /locus_tag="VEP73_09960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT26793.1"
                     /translation="VTTLHGTDVRLASSRPAARAACARVLRASAVVTAVSTWLADAAA
                     GFAPDLAGRIRVAPMPVDDTLFAPGEGPRTELLFVGRLDEQKALDTALRAVARMRGPA
                     AALPLRVVGSGPAEDRLRRLAADLGVAGRIEWEAHLPQQELAGRYRRAAALLVPGRDE
                     GLGLVAVEAQLAGAPVVAAASGGLLDVVRDGDTGRTFPPGDGAALARVVEDVLADPPA
                     AAGLAARGRRAAAARFGRRAAARAYAAVYAEARAAACGAAR"
BASE COUNT          366 a          966 c          923 g          279 t
ORIGIN      
        1 cacggcgacg cgcgcagcat caagtacaac cacggctcgc tgtacatggt ggtccagcag
       61 ctggccaagg ccggcttcgt cgtcgcgcag gaaaccaccc gcgacggggg gcgcccggaa
      121 cgcaccgtgt acgcgctcac cgacgccggc cggcacgaac agcgcgactg gctgcgcgag
      181 ctggtcgagg agccgcagca cgagtacccg cagttcgggg ccgcgctgtc gctgatcgcg
      241 gcgctgccac caggcgaggc cgtcgagctg ctgcgcaggc ggcttgaccg gctcgccgag
      301 cagcgcgccg agatccgcgg cctgatcgac cgcacgctgg ccaccggcgt gcacccgctg
      361 ttcctggtcg aggaggacta ccggctcgtg ctgctcgacg ccgagtcggt gttcgtcgag
      421 cggttcgtcg aacaggtcaa ccgcccggag accggctggg gccgaccctg gggcgagttc
      481 cacggcgagc ccgccaacac cgacgaaagg cagccgtcat gacccacacc cgcaccgccc
      541 tggtcatcgg cggcggcatc gccggcccgg tcgccgcgat ggccctgcgc cgagccggga
      601 tccaggccac cgtgtacgag gcgcatccca gcggcgccga gagcgtcggg ttgttcctca
      661 cgctggcgtc caacgggatc gacgcactgc ggacgatcga cgcagacagg cccgcgctgg
      721 cggccgggtt cccgacaccg gggatcacgc tgcgcagcac gaccggcaag cacctgggcg
      781 cggcgcgcac cggcctgtcg ctccgggacg gcacgaccag ccacacgctc aagcgcgccg
      841 acctctacaa ggcggtccac gacgaggccg cctcccgcgg catcgtggtc gagcacggca
      901 aacgcctgat cgacgtcgag cagaccggcg atggcgtgcg ggcgctgttc gcggacggct
      961 ccgaggccac cggtgaggtg ttgatcggtg ccgacggcat ccactcggtg gtccgcgggc
     1021 tcatcgaccc gaacgcgccc gcacccgcct acgtcggcct ggtcaacctc ggcgggtacg
     1081 cccgcggggt gcacgtggat gccgagcctg gcagctacac catgatcttc ggcaaacggg
     1141 cgttcttcgg ctacgccctg gcaccgggcg gtgaggtgtg gtggttcgcc aacgtgcccc
     1201 gcggcgacga gcccgaccgc ggcgaggtcg aagcgatcac cgccgcggaa tggcaacgcc
     1261 agcttgccga actgtacgcc gaggacgccg ggcccgcggt ccggctcgtc caggccagcg
     1321 acaccgccga cgtcatgaag gccagcccca cccactccat cccgcacctg ccggtctggc
     1381 acaccggccg catgatcgtc atcggggacg cagcccacgc ccccacaccg tcgtccggcc
     1441 agggcgcctc gctgtcggtc gaagacgccg tggcgctcgc caaatgcctg cgcgacctgc
     1501 cccaccagga ggcgttcgcg gcgtacgaga ggatgcgccg agcccgcgtc gagcggatcg
     1561 ccaaggtggc cgcccgggtc aacagcagca aggccgccgg gcccgtcgcc cgggtgttcc
     1621 gggacgcgac cctcccggtc atcttgaaga tgaccgccaa cagcaaacag ctcaacgagc
     1681 aataccgcta ccacatcgac tggaacacgc cgatcagctc ggtcaccgcc tagcagaccc
     1741 tgcccagccg aggctcaccg tgctgctccg catgcggcgg cccgcgcctc ggcgtagacg
     1801 gccgcgtagg cgcgggccgc ggcccggcgg ccgaagcggg cggcggccgc ccgccgcccc
     1861 cgggcggcta ggccggccgc cgccggcggg tcggcgagca cgtcctccac cacgcgggcg
     1921 agggcggccc cgtccccggg cgggaaggtc cttccggtgt cgccgtccct gacgacgtcg
     1981 agcaggccgc ccgaggcggc cgcgaccacc ggcgcgcccg cgagctgcgc ctcgaccgcc
     2041 acgagcccga ggccctcgtc gcgcccgggc acgagcaggg cggccgcgcg gcggtagcgg
     2101 ccggccagct cctgctgggg caggtgagcc tcccactcga tcctgccggc cacgccgagg
     2161 tcggcggcga ggcggcggag ccggtcctcc gccggcccgc tgcccaccac ccggagcggg
     2221 agggccgccg ccgggccccg catgcgggcg acggcgcgca gcgcggtgtc gagggccttc
     2281 tgctcgtcca ggcggccgac gaacagcagc tcggtgcgcg ggccctcccc tggagcgaag
     2341 agcgtgtcgt cgaccggcat gggcgcgacg cggatgcggc cggcgaggtc gggggcgaag
     2401 ccggcggcgg cgtcggccag ccaggtcgac acggcggtca cgacggccga ggcgcgcagg
     2461 accctggcgc acgccgccct ggccgccggg cgggagctgg cgaggcggac gtcggtcccg
     2521 tgcagggtgg tgac
//
LOCUS       DASPYY010000633         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_385635, whole genome shotgun sequence.
ACCESSION   DASPYY010000633 DASPYY010000000
VERSION     DASPYY010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385635"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VEP73_09965"
     CDS             <1..347
                     /locus_tag="VEP73_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HYT26794.1"
                     /translation="IVAEVATLGEDPGQDAERCAGQAARLFGRYVSVLLELAGEEPGD
                     VEVPADPVAASYVVAAGLQVDLADKQRLLLAPTAAERLRAASALLRRELAIMDRLQAA
                     GPAPMAGPFSLN"
     gene            543..1442
                     /locus_tag="VEP73_09970"
     CDS             543..1442
                     /locus_tag="VEP73_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26795.1"
                     /translation="MTARERQRLTGPLDVAASLAGFRRNGDDLIDRWDGRVWLRAVPV
                     GGRMVAAACRPAGTVAAPALDVEVEAGGDPAAVGRAVAGVFVRADGPLAELARRDPVV
                     ARLDRRFPGVRPVLQPDLLTAVVRSISAQQVNLAWAATTRRRLAERFGRRHLLASGEV
                     YSLDAAALAAAPPAELRALQFTTRKAVYITSFARAVASGAVTVAELAALPDEQVVDRL
                     VAFPGIGRWTAEWFLARTLGRPRVVAGDLGVRRAVGAAYLDGRMPAEEEVRAATAHWG
                     AAAGVAQQLLLHWRGAARPGGRP"
BASE COUNT          158 a          588 c          647 g          201 t
ORIGIN      
        1 acatcgtcgc ggaggtggcg acgctcggcg aggacccggg ccaggacgcc gagcggtgcg
       61 ccggccaggc cgcccggctg ttcggcaggt acgtctcggt cctgctcgag ctcgccgggg
      121 aggagccggg cgacgtcgag gtcccggccg acccggtggc cgcctcgtac gtcgtcgcgg
      181 ccgggctgca ggtcgacctg gccgacaagc agcggctgct gctcgccccg acggccgccg
      241 agcggctcag ggccgcctcg gccctgctgc gccgcgagct ggctatcatg gaccgcctgc
      301 aggcggccgg tccggccccg atggccggcc cgttctcgct gaactgatcc gctccgcgag
      361 cctgcgccgc gcccgggcgc gggccggtcg cgccggatcc gggcgttcac cgacctggag
      421 gaggacgtgc gccagtccgt tgcgagggtc gaggcgagcc cgttcctgtc cctcatcgcc
      481 gaggtccgcg gcttcgtgta cgagtcgcac tcgggtcgcc tgcacgaggt cccgggagga
      541 cggtgaccgc cagggagcgg cagcggctga ccgggccgct cgacgtcgcg gcctcgctgg
      601 ccgggttccg ccgcaacggc gacgacctga tcgaccgctg ggacgggcgg gtgtggctgc
      661 gcgccgtgcc ggtcggcggg cggatggtgg ccgccgcctg ccggccggcc gggacggtgg
      721 cagcgccggc gctcgacgtg gaggtggagg cgggcgggga cccggccgcg gtcgggcggg
      781 ccgtggccgg ggtgttcgtc cgggccgatg gacccctcgc cgagctcgcg aggcgagacc
      841 cggtggtcgc ccggctcgac cggcggttcc ccggggtccg tccggtcctc cagcccgacc
      901 tgctcaccgc ggtcgtccgc tccatcagcg cccagcaggt gaacctcgcg tgggcggcca
      961 ccacgcggcg ccggctcgcc gagcggttcg gccgccgcca cctgcttgcg agcggggagg
     1021 tctacagcct ggacgcggcg gcgctggccg cggcgccacc ggccgagctg cgcgccctgc
     1081 agttcaccac ccgcaaggcc gtctacatca cctcgttcgc ccgggcggtg gcctccggag
     1141 cggtcacggt ggccgagctc gccgcgctgc ccgacgagca ggtggtcgac cggctggtcg
     1201 ccttccccgg gatcgggcgc tggacggccg agtggttcct cgcccgcacc ctcgggcggc
     1261 cgcgggtggt ggccggggac ctcggggtgc gcagggcggt cggcgccgcc tacctcgacg
     1321 ggcggatgcc ggccgaggag gaggtgcggg cggcgaccgc ccactggggc gcggcggccg
     1381 gggtcgccca gcagctcctg ctccactggc gaggtgccgc ccgcccgggc gggcggccgt
     1441 aggcccgacc cggccggcca cccgttccgc ctcgcctggg agtagccggc gggatacgtt
     1501 gccgcgcggc gccgcggtca cggaggtacg cgatcacgga ggtagaggtg gccggcgacg
     1561 atgctggtcg acgatgctgg tcgacgatgc tggt
//
LOCUS       DASPYY010000634         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2774900, whole genome shotgun sequence.
ACCESSION   DASPYY010000634 DASPYY010000000
VERSION     DASPYY010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774900"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1115
                     /locus_tag="VEP73_09975"
     CDS             150..1115
                     /locus_tag="VEP73_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT26796.1"
                     /translation="MWRYILRRLMWVIVVLLVITLITFVIFFVMPSVDPAVAFAGRQP
                     TPEIVAEVKRQFGLDHPVWEQYLLFVRHLFLGDRYGWPGLGFSFASRSAIKPELFSRF
                     LITAQLAVGAALVWLSIGIPIGVASAVRRRSLLDRAAMAFALFFVSMPVFLLGLLALW
                     LLWFKWHLTPGTGYISVGTNFGSWLGHMILPWFVLAMLFAAFYARMTRGNLIETMAQD
                     YIRTARVKGLPEWKVILKHGLRASLTPVVTMFGMDLGSLFGTAVVTESVFNLQGMGQW
                     AIISVFAGDIPTVLAVVSVTAVAIALCNLIVDVLYAYLDPRVRYS"
     gene            complement(1336..>2339)
                     /locus_tag="VEP73_09980"
     CDS             complement(1336..>2339)
                     /locus_tag="VEP73_09980"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYT26797.1"
                     /translation="YHAYLQWLLDEQLAAVPRMRVGLLNDLAVGVDPGGFDAWSFQRA
                     IASGITVGAPPDELGPEGQDWGFAPFVPAHLAACGYAPFTQTIRAGLRHAQGLRVDHV
                     MGLFRLFWIPQGRPPTKGTYVRYPADDLLGILALESHRAAWLVVGEDLGTVEPGVRER
                     LADEDVLSYRLAWFERGPDGAGRRRAADYPRLALAAATTHDLPTVAGFLSGADLAHLR
                     EIGAVPPGQADEAAERRREDAASLLGLLEEEGLLDPGGGRSVGTVVAALHAFLARTPS
                     MLVAATLEDAVQAVERPNVPGTVDERPNWSLPLPVLLDDLPGDPRARRLAEALAAVG"
BASE COUNT          300 a          806 c          888 g          345 t
ORIGIN      
        1 gtgacccact acgagttcga ccagttctcg gcggagccgt cactggtcaa catcgcggtg
       61 aacaacaagg agaagctcgg ctcgtagcac ggacggggcg gggccggcgc tgacggcggc
      121 cccgccccct cagcaacggg ggcagggaca tgtggcgcta catcctccgg cggctcatgt
      181 gggtcatcgt cgtgctgctg gtcatcaccc tgatcacctt cgtgatcttc ttcgtgatgc
      241 cgtcggtgga cccggcggtc gccttcgcgg gcaggcagcc gaccccggag atcgtcgccg
      301 aggtcaagcg ccagttcggc ctggaccatc cggtgtggga gcagtacctg ctgttcgtgc
      361 ggcacctgtt cctcggcgac cgctacggct ggcccggcct cggcttctcg ttcgccagcc
      421 gctcggcgat caagccggag ctgttcagcc gcttcctcat caccgcgcag ctcgccgtcg
      481 gcgcggcgct cgtgtggctg tccatcggca tccccatcgg ggtcgcctcg gcggtcaggc
      541 gccgctcgct gctggaccgc gcggccatgg cgttcgcgct gttcttcgtg tcgatgccgg
      601 tgttcctgct cggcctgctg gcgctgtggc tgctgtggtt caagtggcac ctgacgccgg
      661 ggaccggcta catctccgtc gggaccaact tcggctcgtg gctcggccac atgatcctgc
      721 cgtggttcgt gctcgccatg ctgttcgccg cgttctacgc gcgcatgacg cgcggcaacc
      781 tgatcgagac gatggcgcag gactacatcc gcaccgcccg ggtcaagggg ctgccggaat
      841 ggaaggtgat cctcaagcac gggctgcggg ccagcctcac gccggtggtc acgatgttcg
      901 gcatggacct cggctcgctg ttcggcaccg cggtggtcac cgagtcggtc ttcaacctgc
      961 agggcatggg ccagtgggcg atcatctcgg tgttcgccgg ggacatcccc acggtgctgg
     1021 cggtggtcag cgtgaccgcg gtggcgatcg cgctctgcaa cctcatcgtc gacgtgctct
     1081 acgcctacct cgacccgcgc gtgcgctaca gctgaccggc acagggtctg atgcagcggg
     1141 gcgcttcagg gggtgcggcg ctggcgccgg aagccggcgt aggcgaggag cccaacgagc
     1201 acgaggccga cgatgggggc gctcacccgt gggctccagg cggcgtgccg cgctggccga
     1261 ggctgcgcgc ctcggccgcg ttggcggtgg ccgccacgat caccttctca agagggggga
     1321 tggcatacct ctggatcagc cgacggcggc gagcgcctcg gcgaggcgcc gcgcgcgggg
     1381 gtcgccgggc aggtcgtcga gcagcacggg gagaggcagc gaccagttcg gccgctcgtc
     1441 cacggtgccc ggcacgttcg gccgctcgac cgcctgcacg gcgtcctcca gcgtggccgc
     1501 gaccagcatg gacggggtgc gggcgaggaa ggcgtgcagc gcggcgacga cggtgcccac
     1561 gctccggccg ccgcccgggt ccagcagccc ctcctcctcg aggaggccga gcaggctcgc
     1621 ggcgtcctca cgccggcgct cggccgcctc gtcggcctgt ccgggcggga cggcgccgat
     1681 ctcgcgcagg tgggcgaggt cggcgccgga gaggaagccg gccacggtcg gcaggtcgtg
     1741 ggtggtggcc gcggcgagcg cgaggcgggg gtagtcggcc gcccggcgcc gcccggcgcc
     1801 gtccgggccc cgctcgaacc aggcgagccg gtaggagagc acgtcctcgt cggccagccg
     1861 ctcgcggact ccgggctcga cggtgccgag gtcctcgccg acgaccagcc aggcggcccg
     1921 gtggctctcg agcgcgagga tgccgagcag gtcgtcggcg gggtagcgga cgtaggtgcc
     1981 cttggtcggc ggccggccct gcgggatcca gaagaggcgg aacaggccca tgacgtggtc
     2041 gacgcgcagc ccctgggcgt ggcgcaggcc ggcccggatc gtctgggtga acggcgcgta
     2101 gccgcaggcg gccaggtggg cgggcacgaa cggggcgaag ccccagtcct gcccctccgg
     2161 gccgagctcg tcggggggcg cgcccacggt gatgccggac gcgatcgcgc gctggaacga
     2221 ccaggcgtcg aagccgccag ggtcgacgcc gacggcgagg tcgttgagca gcccgacgcg
     2281 catccggggg accgcggcca gctgctcgtc gaggagccac tgcaggtagg cgtggtaac
//
LOCUS       DASPYY010000635         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_386103, whole genome shotgun sequence.
ACCESSION   DASPYY010000635 DASPYY010000000
VERSION     DASPYY010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386103"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1131)
                     /locus_tag="VEP73_09985"
     CDS             complement(112..1131)
                     /locus_tag="VEP73_09985"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYT26798.1"
                     /translation="MNKHLLSAGDLSAADITRVLDTAAHLAHVTDRAIKKLPTLRGRT
                     VCNLFFEDSTRTRISFELAAKRLSADVINFSARGSSVAKGESLKDTAITLEAMGVDAI
                     VIRHGASGAPWRLAEWVDAVVVNAGDGTHEHPTQALLDLYTMRQRFPSFDGLRVAIVG
                     DVLHSRVARSNVLALTTMGAEVTLVAPPTLLPARVDGWGATVSYDLDAVLPKQDVVML
                     LRMQLERQRAGFFPSLREYARLYGLDARRLDAMAPHAIVMHPGPMNRGVEIAADVADS
                     IERSVIASQVTNGISVRMGLLYLLLGGPAVAGPGGAAGSGATGQDAGSAAVAAAGARA
                     RGGPW"
     gene            complement(1128..>1521)
                     /gene="pyrR"
                     /locus_tag="VEP73_09990"
     CDS             complement(1128..>1521)
                     /gene="pyrR"
                     /locus_tag="VEP73_09990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HYT26799.1"
                     /translation="RDDLSLIAQQPLVKGTDLPFNLNESTIVLVDDVLYTGRTIRAAL
                     DALVDFGRPHAIQLAVLVDRGHRELPLRADYVGKNVPTATDEAVRVRLAETDGVDGVV
                     IRRGPAGRERPVRAPSARPPAARGEARA"
BASE COUNT          198 a          544 c          579 g          200 t
ORIGIN      
        1 ggcgctcccc cacctccgcg acgacgccgt cgcggaccag gacgtcgagc cggtcgtcgc
       61 gcctggaggc cgggtcgatc agccggccgc cgcgcagcag caccgcccgg ctcaccacgg
      121 cccccctcgc gccctcgcac cggccgccgc gaccgccgcg gacccggcgt cctggccggt
      181 ggcgccgctg cccgccgcac cgccgggccc cgcgacggcg gggccgccga gcagcaggta
      241 gagcaggccc atgcggacgc tgatgccatt ggtgacctga ctcgcgatca ccgagcgctc
      301 gatcgagtcg gcgacgtcgg cggcgatctc cactccccgg ttcatcgggc cggggtgcat
      361 cacgatggcg tgcggcgcca tcgcgtccag gcggcgcgcg tccaggccgt acaggcgtgc
      421 gtactcgcgc aggctgggga agaagccggc ccgctggcgc tcgagctgca tccgcaggag
      481 catgacgacg tcctgcttgg gcaggacggc gtccaggtcg taggagacgg tcgcccccca
      541 cccgtcgacc ctggccggca gcagcgtcgg cggcgccacc agggtgacct ccgcgcccat
      601 cgtcgtgagg gcgagcacgt tggaccgggc cacccgggag tggagcacgt cgccgacgat
      661 cgcgacccgg agcccgtcga agctcgggaa gcgctgccgc atcgtgtaga ggtcgagcag
      721 cgcctgggtg ggatgctcgt gggtgccgtc gccggcgttg acgaccacgg cgtcgaccca
      781 ctcggcgagg cgccatggcg cccccgaggc accgtggcgg atcacgatcg cgtccacgcc
      841 catcgcctcg agggtgatcg cggtgtcctt caggctctcg cccttggcga cgctcgagcc
      901 cctggccgag aagttgatga cgtcggccga gagccgcttc gcagccagct cgaacgagat
      961 gcgcgtgcgg gtcgagtcct cgaagaacag gttgcagacg gtccgcccgc gcagcgtggg
     1021 cagcttcttg atggcccggt cggtgacgtg cgcgaggtgg gcggcggtgt cgagcacgcg
     1081 ggtgatgtcc gcggcagaca ggtcgccggc cgagagcagg tgcttgttca tgcgcgtgcc
     1141 tccccgcggg cggcgggcgg ccgggccgag ggggcccgga cgggtcgctc ccggccggcc
     1201 gggccgcgcc ggatcacgac gccgtcgacg ccgtcggtct cggccaggcg gacgcggacc
     1261 gcctcgtccg tcgcggtggg cacgttcttg cccacgtagt cggcccggag cggcagctcc
     1321 cggtggcccc ggtcgacgag gacggcgagc tggatggcgt gcggcctgcc gaagtcgacg
     1381 agcgcgtcga gcgcggcccg gatcgtgcgg ccggtgtaca ggacgtcatc cacgaggacg
     1441 atcgtggact cgttcaggtt gaacggcagg tccgtgccct tcacgagggg ctgttgggcg
     1501 atcagcgaca ggtcgtctcg a
//
LOCUS       DASPYY010000636         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2634560, whole genome shotgun sequence.
ACCESSION   DASPYY010000636 DASPYY010000000
VERSION     DASPYY010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634560"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VEP73_09995"
     CDS             complement(<1..1806)
                     /locus_tag="VEP73_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT26800.1"
                     /translation="MSSTWRSFVPDYVVRMLLDDPDDDPDEVPAARAEWMEAVVLFAD
                     VAGFTAMSEAFARFGRYGTEQLTQILNGYFGATIDLVSRYGGSVAKFAGDAITAVFPY
                     DRPSRRAARRRALQCAVDMQATMVHYETLVTPAGRFGLAMKAGLAAGRVLSTIVGDPA
                     IRLEYVLAGRPLDRATTAEHHAAKGEVVVDAGLLGDGTGVDTVERADGLYLVARLTRR
                     VPARPLPRVEDVPPARLVPFLHPDIAERLRAGRQGLVNEHRKVTAAFAGFAELVSDDP
                     QAVDRLQRFMAAAVGRIQRYGGYLRQVDTGDKGSLLVVLFGAPISHEDDEERAVHCCL
                     ELLGLPDGATAAGITTGHAYCGEVGSDTRREYAAIGDSVNLAARLLQAARPGQLLIDT
                     PTHERVTETAIQDRLEPVTVKGKTGPTAIWAVRAVHDRAGFPRRVPGARQPLVGRTDE
                     VATARALVERAVAGNGQVLGLAGDAGIGKSRLAEEVVRIAEALGCTAYGGACRSHGTT
                     TSYLVWRSIWRGLLELDVSLPIAEQQAQLTAQIARRDGGSGQRAPLLAPVVNLPMPDS
                     ELTAQLDPQTRGELLRSLLLDCLRGRAGAGPLLLML"
     gene            2001..2612
                     /locus_tag="VEP73_10000"
     CDS             2001..2612
                     /locus_tag="VEP73_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26801.1"
                     /translation="MPQCTSRLAIYVLQACLDGIEFDLALTPEFPPYSEEYPQTSSDE
                     QLRNPTITAATPVPRRKGGGNWKNAILRLDPFNVIRWKDEDVPPLNSPAKDHIVLQQR
                     LKLTPAWGALQTAGTQRWHPLSCPSGPYPQPRSGYGEKVSDYRRFRLNWNRKLKAGDE
                     VAISFEGFKAGRASVEYIVFIHDGLLDAAPPWQDASTPDAAGE"
     gene            2710..>2975
                     /locus_tag="VEP73_10005"
     CDS             2710..>2975
                     /locus_tag="VEP73_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HYT26802.1"
                     /translation="MDASEYWNPKTETLGREELEALQLAKLRRLAEWAAARSPFYARS
                     FRRAGFRPEQLETLADLRRLPYLTRDEWMASQAERPPFGTLPVAG"
BASE COUNT          430 a         1064 c         1070 g          411 t
ORIGIN      
        1 gagcatcagc aggagcggcc cggccccggc ccgaccgcgc aggcagtcga gcaggagcga
       61 ccggagcagc tcgccccgcg tctgcgggtc gagctgggcg gtcagctcgc tgtcgggcat
      121 gggcaggttg accaccggcg cgagcagggg cgcccgctgg ccggagccgc cgtcgcgccg
      181 ggcgatctgg gcggtgagct gtgcctgctg ctccgcgatg ggcagcgaga cgtcgagctc
      241 gaggagaccg cgccagatcg accgccagac gaggtagctg gtggtggtgc cgtgggagcg
      301 gcaggcgccg ccgtaggcgg tgcacccgag cgcctcggcg atgcggacca cctcctcggc
      361 gagccgggac ttgccgatcc cggcatcgcc ggcgaggccg agaacctggc cgttgcccgc
      421 cacggcccgc tccacgagcg cccgggccgt ggcgacctcg tccgtcctgc ccaccagcgg
      481 ttgcctggct cccggcaccc gtcgggggaa tccggcccgg tcgtgcaccg cgcggaccgc
      541 ccagatggcg gtgggcccgg tcttcccctt gaccgtgacc ggctcgaggc ggtcctggat
      601 ggcggtctcg gtcacccgct cgtgggtcgg cgtgtcgatg aggagctggc ccggcctggc
      661 agcctgcagg agccgggcgg cgaggttcac ggagtcgccg atcgcggcgt actcccgccg
      721 ggtgtccgag ccaacctcgc cgcagtatgc gtggccggtc gtgatccccg ccgcggtcgc
      781 cccgtccggc agcccgagca gctcgaggca gcagtgcacc gcgcgctcct cgtcgtcctc
      841 gtggctgatc ggggctccga acaggaccac cagcaggctg cccttgtcgc cggtgtcgac
      901 ctggcggagg tagccgccgt agcgctggat cctgccgacg gcggcggcca tgaagcgctg
      961 gagccggtcg accgcctgcg ggtcgtcgct gacgagctcg gcgaatcccg cgaaggcggc
     1021 cgtgaccttg cggtgctcgt tcaccaggcc ctggcgcccc gcccgcagcc gctcggcgat
     1081 gtcgggatgc aggaagggga ccagccgtgc cggcgggacg tcctcgaccc gcgggagcgg
     1141 gcgtgccggc acccggcggg tcaggcgggc gaccaggtag agcccgtcgg cccgttcgac
     1201 cgtgtcgacg cccgtgccgt cgccgaggag ccccgcgtcg acgacgacct cgcccttggc
     1261 ggcatgatgc tcggccgtgg tggccctgtc cagcggccgg cccgcgagga cgtactccag
     1321 ccggatggcg gggtcgccca cgatcgtcga gagcacccgg ccagcggcga gcccggcctt
     1381 catggcgagg ccgaacctcc ccgcgggggt gacgagggtc tcgtagtgga ccatggtcgc
     1441 ctgcatgtcg accgcgcact ggagcgctcg ccggcgggcc gcccggcggc tgggtcggtc
     1501 gtagggaaac acggcggtga tcgcgtcgcc ggcgaacttc gccacgctcc cgccgtacct
     1561 cgagacgagg tcgatcgtgg cgccgaaata gccattgagg atctgggtca gctgctcggt
     1621 gccgtacctg ccaaatcgag caaatgcttc gctcatcgcg gtgaacccgg cgacgtcggc
     1681 gaagagcacg accgcctcca tccactcggc gcgagcggcg ggcacctcgt ccggatcgtc
     1741 gtccggatcg tcgagcaaca tccggacgac gtagtcgggg acgaacgagc gccaggtcga
     1801 gctcaacgct cctcctcgtg acgaccagag cgccgacgtc gtcgcgggtt cctccggacg
     1861 gtcagccctc atgttctcca gacccggcac ggttacgcag ttctgcctag ttgtggcaag
     1921 ggtggcattg gtgcgtagta gggcggacag tcgacgcgaa ggtctctaga ccccgactag
     1981 cctcagaaag gtgggcaacc gtgccgcaat gcaccagtcg tctggcaatc tacgtcctgc
     2041 aggcgtgcct ggatggtatc gagttcgacc tggcgctgac accagaattc ccaccgtact
     2101 cggaggaata cccgcagacg tcgtccgacg agcagctgcg gaacccgacg atcacggcgg
     2161 cgacaccggt cccgcgccgg aagggcggcg gcaactggaa gaacgcgatc ctgcggctcg
     2221 atccgttcaa cgtcatccgc tggaaggacg aggacgtccc gccactcaac agtccggcca
     2281 aggaccacat cgtgctccag cagcggctca agctcactcc tgcgtggggt gcgctccaga
     2341 ccgcgggcac ccagcggtgg catcccctgt cctgtccctc ggggccctac ccgcaaccga
     2401 ggtccggcta cggcgagaag gtcagtgact accgccggtt caggctcaac tggaacagga
     2461 agctcaaggc cggcgacgag gtcgccatca gcttcgaggg gttcaaggcc gggcgggcca
     2521 gcgtcgagta catcgtcttc atccacgacg gcctcctcga cgcggctccg ccgtggcagg
     2581 acgcctcgac gccggacgcg gccggggaat gacctcctcg ggcgcgcccg ccgcttcaac
     2641 ccggcgcggg ccggccgtat gcttgcggcc agggaccgtt cgcaccggac gacgaggtcg
     2701 ggatgcccga tggacgcctc cgagtactgg aacccgaaga ccgagacgct cggccgtgag
     2761 gagctcgagg cgctccagct cgccaagctg cggcggctgg ccgagtgggc ggccgcgcgc
     2821 agccccttct acgcccgctc gttccggcgc gccggcttcc gccccgagca gctcgagacc
     2881 ctcgccgacc tgcgccggct gccgtacctg acccgcgacg agtggatggc gtcccaggcg
     2941 gagcggccgc cgttcgggac cctgccggtc gccgg
//
LOCUS       DASPYY010000637         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_669848, whole genome shotgun sequence.
ACCESSION   DASPYY010000637 DASPYY010000000
VERSION     DASPYY010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669848"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEP73_10010"
     CDS             complement(<1..465)
                     /locus_tag="VEP73_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYT26803.1"
                     /translation="MATGGGVPAAAAGGGTQALLDAWFATAPAGIAVLDTELRIVRAN
                     EALAEAAGRPVRALVGRTLPAAVPGLPTEVPAVLHRVLATGEPVVDHELRVDAGGGRG
                     RHWLASFHPVRGDDGPVLGIGVIVQEITERKLAEERLAFQATHDALTGLPNRS"
     regulatory      547..675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            816..>1873
                     /gene="ribD"
                     /locus_tag="VEP73_10015"
     CDS             816..>1873
                     /gene="ribD"
                     /locus_tag="VEP73_10015"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYT26804.1"
                     /translation="MARALALAEGGQGTTSPNPMVGAVLVRGGRVVGEGFHAAPGRPH
                     AEAEALAAAGPAAVGATCYVTLEPCVHHGRTPPCADALVDAGVARVVAAVTDPDPHVR
                     GAGFARLRAAGIEVAAGVLAAAAEEQNAAFLTHRRLGRPRCTLKAAASLDGKVAAADG
                     TSQWITGPEARGDAHLLRARADAIAVGAGTALADDPRLTVRLAPRPGRPPLRVLVDAA
                     GRVGATGRLFDGEAPTLVATTAAAPPAARAAWEAAGAEVLVCPPSGPGTDGRSGAGAP
                     GVDLGWLAAALGGRGVLELLVEGGPRLHASLWAAGLADRLVWYLAPLAIGGDGAPGLL
                     GDGGAATLAAARRLRIASV"
BASE COUNT          179 a          765 c          737 g          192 t
ORIGIN      
        1 cgagcggttg ggcagcccgg tgagcgcgtc gtgggtggcc tggaacgcga gccgctcctc
       61 ggccagcttc cgctcggtga tctcctgcac gatcacgccg atcccgagca ccggcccgtc
      121 gtcgccccgc accgggtgga agctcgcgag ccagtgccgg ccgcggccgc cgccggcgtc
      181 gacccgcagc tcgtggtcga cgaccggctc gccggtggcc aggacccggt gcaggacggc
      241 ggggacctcg gtgggcagcc cggggacggc ggcgggcagg gtacgcccga cgagggcgcg
      301 caccggccgg ccggccgcct cggcgagcgc ctcgttggcg cggacgatgc gcagctcggt
      361 gtccaggacg gcgatgccgg ccggcgcggt cgcgaaccag gcgtcgagca gcgcctgcgt
      421 cccgcccccg gccgcggccg ccgggacgcc gcctccggtc gccaccgccc accccttccc
      481 cttccccagg tccggccgcc ctcgcgggtc ccgccggccc ggtgctacgc tgcttgcaca
      541 gagtacgtac gtctccgggg cggggtggaa ctccccaccg gcggtgagag cccgcgaccc
      601 ccgagccgcc agctcggggt ggaccctgtg cgatccaggg gccgacggtg acagtccgga
      661 taggaggagg cgggcggacc agcggccgtg tgccgcgggc cgggcgtgac gcccgagccc
      721 gcccggcgac gccaggctgg ccggctaccg cgccccggga ccgaccgggg agctggcagg
      781 tggcgcgagg agaccggcgc gaggacgagg cctggatggc ccgcgcgctc gcccttgccg
      841 agggcggcca gggcaccacc agtcccaacc cgatggtggg cgccgtcctg gtgcgcgggg
      901 gccgggtcgt cggggagggg ttccacgccg cccctggcag gccgcacgcc gaggccgagg
      961 cgctcgccgc cgccggcccg gccgccgtcg gcgccacctg ctacgtgacg ctcgagccgt
     1021 gcgtgcacca cggccgcacc ccgccctgcg ccgacgccct ggtcgacgcc ggggtggcca
     1081 gggtcgtcgc cgccgtcacc gaccctgacc cgcacgtccg gggcgccggg ttcgcgcgcc
     1141 tgcgcgccgc cgggatcgag gtcgcggccg gcgtgctcgc cgcggcggcc gaggagcaga
     1201 acgccgcctt cctcacccac cggcggctcg gacggccccg ctgcaccctc aaggccgcgg
     1261 ccagcctcga cggcaaggtc gcggccgccg acggcacgtc gcagtggatc accggccccg
     1321 aggcgcgcgg cgacgcccac ctcctccgcg cccgggcgga cgcgatcgcg gttggagcgg
     1381 gcacggcgct cgccgacgac ccgcgcctga ccgtccgcct cgccccgcga cccggccggc
     1441 cgcccctgcg ggtcctggtc gacgcggccg ggcgcgtggg cgccacgggc cgcctgttcg
     1501 acggtgaggc gcccacgctg gtcgcgacga cggccgccgc gccgcccgcc gcccgcgcgg
     1561 cctgggaggc ggccggtgcc gaggtgctcg tctgcccgcc gtccgggccg ggcactgacg
     1621 gcaggtccgg ggcgggcgcc cccggcgtgg acctcggctg gctcgcggcc gcgctcgggg
     1681 ggcgcggcgt gctcgagctg ctcgtcgagg gaggcccccg gctgcacgcg agcttgtggg
     1741 cggccggcct cgccgatcgc ctcgtctggt acctggcgcc gctcgccatc ggcggcgacg
     1801 gcgcgcccgg cctgctcggc gacggcggcg ccgccaccct cgccgccgcc cgccgcctgc
     1861 gcatcgcgtc ggt
//
LOCUS       DASPYY010000638         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_105389, whole genome shotgun sequence.
ACCESSION   DASPYY010000638 DASPYY010000000
VERSION     DASPYY010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105389"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VEP73_10020"
     CDS             <1..762
                     /locus_tag="VEP73_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYT26805.1"
                     /translation="SARLAAPLSGKHLTAPAPPPPIFSKMPAPLGAPRPEPGTPASLL
                     DHVAAVVRRLLDADCSKIYELAATNGLLLLRAGAGWAPRYIGRTTVPAGTGSPAGLAI
                     ATRKPVIVADCLTERRCAIPPLLRDHGVRSTANVPITAGAHVYGALSVGAKRLRTFTA
                     GEVRVLEAFADLIGATATVPRPDPRIHRLGEREREVLSLVARGYTSRAIAARLAVSAK
                     TVETYRVRLARKLDVRSRPELVRLALHAGLLNGEG"
     gene            926..1315
                     /locus_tag="VEP73_10025"
     CDS             926..1315
                     /locus_tag="VEP73_10025"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HYT26806.1"
                     /translation="MESAMLLVAVRLGLWIAPFASLRRWTARLARPRDTSPGAVPLPA
                     ERIAWAVSAVGRRVPGGRNCLARALTAQTLLARYGHPARLRIGVAHTTERGFHAHAWV
                     ETRDGVVLRESALERFTALPALDGEQL"
BASE COUNT          206 a          657 c          486 g          208 t
ORIGIN      
        1 tccgcgagac tagcggcgcc cctgtcagga aaacacctga cggcaccagc cccaccgccg
       61 cctatattct cgaaaatgcc tgcccccctc ggcgcgcccc gccccgagcc cggcacgccc
      121 gcatccctcc tcgaccacgt ggccgccgtc gtccggcggc tgctcgacgc cgactgctcc
      181 aagatctacg agctcgccgc cacgaacggc ctcctcctgc tccgcgccgg cgccggctgg
      241 gccccccgct acatcggccg gacgaccgtg cccgccggga ccggctcccc cgccggcctc
      301 gccatcgcca cgcgcaaacc cgtcatcgtc gccgactgcc tcaccgaacg ccgctgcgcc
      361 atcccccccc tgctgcgcga ccacggcgtg cgcagcacgg cgaacgtccc gattaccgcc
      421 ggtgcgcacg tctacggcgc gctcagcgtc ggcgccaaac ggctccgcac cttcaccgcc
      481 ggtgaggtcc gggtgctcga ggccttcgcc gacctcatcg gcgccaccgc caccgtcccc
      541 cggcccgacc cgcgcatcca ccgcctcggc gagcgcgagc gggaggtcct gagcctcgtc
      601 gcccggggct acaccagccg cgccatcgcc gcccggctcg cggtcagcgc caagaccgtc
      661 gaaacgtacc gcgtccggct cgcccgcaag ctcgacgtcc gctcccggcc cgagctggtc
      721 cgcctcgccc tgcacgccgg actgctgaac ggcgaagggt gaccggctcg cccctgtagg
      781 ggaattcacc gatagccgac ccccgccgac cagccacatt ctgggcccat gccggcgctc
      841 gaccacgtgg agcgcttgat cgaggtgcgc cacgactgcg ttcattaagt tccttcgcct
      901 gccgccgcgg gagcggcgcg tcctcatcga gtccgcgatg ttgctcgtcg ccgtgcggct
      961 cggcctgtgg atcgcgccgt tcgcctccct gcggcgctgg acggcccgcc tcgcgcgccc
     1021 gcgcgacacc tcgcccggtg cggtgccgct gcccgccgag cgcatcgcct gggccgtgag
     1081 cgccgttggc cgccgcgtgc cgggaggccg caactgcctc gcaagagccc tgacggccca
     1141 aactctcctc gcccgctacg gtcacccggc ccggctccgt atcggagtcg cccacacgac
     1201 ggagcgggga ttccacgcgc acgcgtgggt cgagacccgg gacggtgtcg tcctccgcga
     1261 atcggccctc gagcgcttca cggccttgcc ggccctcgac ggagagcaac tgtgaacgcc
     1321 ccggcccggc cgttcgcctg cgagctgcac ggactacgca tccgttcgga gataccgctg
     1381 ggcggcagcg cgtgcgctga ggagtcgcct gacctcaccg tatcctgggg cgccgggccg
     1441 acgccaccgc tcacggaacc ggtcggacgc gttatggcga gcttcgcctg gaacggcggg
     1501 cccggctaca cccacacgca aacggacacg gggtacaccc tgcggttcca cggcctg
//
LOCUS       DASPYY010000639         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_530595, whole genome shotgun sequence.
ACCESSION   DASPYY010000639 DASPYY010000000
VERSION     DASPYY010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530595"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1416)
                     /locus_tag="VEP73_10030"
     CDS             complement(250..1416)
                     /locus_tag="VEP73_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYT26807.1"
                     /translation="MAGTRDTSAPPAGTAAGDSPDRHQVRGQAAYRWGVFACLGVLTV
                     LAAVYAVYVARAVLVRALVALFFAVSLDPAVRWLTRRGMGRGTAVTIIFVLVLAVVVA
                     FLVSIIPPLVSQFQTLATDLPRYLDQLSRRSREFRVLNRQLDVSRQLQSLVSTLPSRL
                     GTGLLGFTSRVFGALFSGLTALVLTIYFMADLPRLQAGLVRLMPRARRARSAEVVGLV
                     VGKVGDYMIGNILISVVAGIASYLAFLVIHVPFAIPLAILVAFTDLIPMVGATLGAVA
                     CVAVAAFAASIWPTAVLAAAFFVAYQQLENYLIAPRILRSTVDISAAAVLLAGLVGAT
                     VLGLVGALMAIPVAAAMKVLYAQRVAAEAAGPATPPADTGPEEGARAVERPAPP"
     gene            1575..2012
                     /locus_tag="VEP73_10035"
     CDS             1575..2012
                     /locus_tag="VEP73_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYT26808.1"
                     /translation="MTEINASNYDELAAAVDRHSNEELVAAIQGQEGGVDGTLERVFQ
                     GMCESFSPQKAGGQQATVQYQIGAPDGQHPWTMRIADGRCEAEKGTAESPRVTLTIGL
                     GDFLRLVTNRLNGMQAFMTGKLKVSGDLFFAQTFASWFERPSS"
     gene            complement(1945..2781)
                     /locus_tag="VEP73_10040"
     CDS             complement(1945..2781)
                     /locus_tag="VEP73_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYT26809.1"
                     /translation="MPGVTKLRLATWNIFSGRTREGDRVDLDLTLATLRALDADVVAL
                     QEVDRDQDRSGRADQARVLADGLGMGWRFAPALSGTPGGAAEPPPPGAPDPGGAAYGV
                     ALLSRLPLGREETVRLPRAGRDEPRVALVAAAGRPTPLTVAATHLTFVPGSNAGQLRA
                     LQRHLAAWPPPRLLLGDLNLWLPLVRAVSLPGWRPLARGRTWPNRPPATVRPEVQLDH
                     VLASGPGVRLRRSRVQAGPASDHRAVVVEVELDLPDPAATTTGARTRTRRSARRTGPR
                     TP"
     gene            complement(2783..3049)
                     /locus_tag="VEP73_10045"
     CDS             complement(2783..3049)
                     /locus_tag="VEP73_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26810.1"
                     /translation="MRSGWPGRLPGTGPTCSPLRNPLTARLRLASRLAMARPVAWAWR
                     AIARLAGVPAPSVRWRVVRGPRFENQVATLELDGRAARVRIERG"
     gene            3556..4047
                     /locus_tag="VEP73_10050"
     CDS             3556..4047
                     /locus_tag="VEP73_10050"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HYT26811.1"
                     /translation="MATTLFLVRHAKAADRAGWSGADRDRPLTGRGRAQAERLALALA
                     PERPVLVAASPWLRCVQTAVPLAEALGAEPRADGRLGYDQPDMAAWVRDAAMHPGRAA
                     VAVGHGDLIPMYLLRAGVLDGLPDFRTGSLFRVRVEDGRPVEAVYVDRRDLHGRGAVP
                     ERP"
     gene            4219..>4521
                     /locus_tag="VEP73_10055"
     CDS             4219..>4521
                     /locus_tag="VEP73_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HYT26812.1"
                     /translation="MGDLQRVGVMGIRGSFSEEAALEYARDNKLGDVKIVELLSTDRV
                     LRAVAVGEVDLGIFALENSNGGVVYESVYAMAAHRFKVLDLFEIDVNHALLGHPSLP"
BASE COUNT          603 a         1658 c         1756 g          504 t
ORIGIN      
        1 caccagcgct gggcctacgc ccaggtccgc cagggcgtgg acgcgccctg gctgctcagc
       61 gagggccacg ccccggtcgg cctctgcggc gactggtgcg agggcgcgaa ggtggagggc
      121 gcgtggcgct ccggccacgg cttgggccac gccctgctcg agcggctcgc ctgacccggc
      181 gggccgcggc gcccgggcat ccctgggcac ggggcctcac ccggtgggga cggtcacggt
      241 gggggccggt cacggtgggg ccgggcgctc gaccgcccgg gcgccctcct ccgggccggt
      301 gtcggccggg ggcgtcgccg ggccggcggc ctccgcggcc acccgctggg cgtagaggac
      361 cttcatcgcg gccgcgaccg ggatcgccat cagcgcgccg accaggccga ggacggtcgc
      421 gccgaccagg cccgcgagca gcacggcggc ggcggagatg tcgaccgtcg accggaggat
      481 gcgcggggcg atgaggtagt tctcgagctg ctggtaggcc acgaagaagg cggccgcgag
      541 gacggcggtc ggccagatgg aggcggcgaa ggcggccacc gccacgcagg cgaccgcccc
      601 cagggtggcg ccgaccatcg ggatcaggtc ggtgaaggcg accaggatcg cgagcgggat
      661 cgcgaacggt acgtggatca ccaggaacgc gaggtaggag gcgatcccgg ccacgaccga
      721 gatgaggatg ttgccgatca tgtagtcgcc gaccttgccg accacgaggc cgacgacctc
      781 ggccgagcgt gcccgccggg cgcggggcat gaggcggacg agcccggcct gcagccgcgg
      841 caggtcggcc atgaagtaga tggtcagcac gagcgcggtg aggccgctga acagcgcgcc
      901 gaacacgcgg ctggtgaatc cgagcaggcc cgtcccgagc cgggagggca aggtgctgac
      961 cagcgactgc agctgcctgg agacgtcgag ctgccggttc agcacccgga actcgcggga
     1021 gcgccggctc agctggtcca ggtagcgggg caggtcggtg gcgagcgtct ggaactgcga
     1081 gacgagcggc gggatgatcg agacgaggaa cgcgaccacc accgccagga cgagcacgaa
     1141 gatgatcgtc acggcggtgc cccggcccat gccgcggcgg gtcagccagc gcaccgccgg
     1201 gtccaggctg accgcgaaga acaacgccac cagggcccgg acgaggaccg ccctggccac
     1261 gtagacggcg tagaccgcgg cgagcacggt cagcacgccg aggcaggcga atacccccca
     1321 ccggtacgcg gcctggccgc ggacctggtg ccggtccggg gagtccccgg cggcggtccc
     1381 cgccggaggg gcgctggtgt cacgcgtccc tgccatctcg catcatcctc tcccgggagt
     1441 ggctcccgtg cggcagcagc gcaagttaag ggcctctgcc ctcccgcctc aagtgcggga
     1501 ccgccgccgc ggctcggggc ggcgtcgcga taggctgagc accgttggcc gccgcccccc
     1561 ggagggacac gggaatgacc gagatcaacg cctcgaacta cgacgagctc gccgccgccg
     1621 tcgaccggca ctccaacgag gagctggtcg cggccatcca ggggcaggag ggcggcgtcg
     1681 acggcacgct cgagcgcgtc ttccagggca tgtgcgagtc gttcagcccg cagaaggcgg
     1741 gcgggcagca ggccaccgtg cagtaccaga tcggcgctcc ggacggccag catccctgga
     1801 cgatgcggat cgccgacggc cgctgcgagg ccgagaaggg cacggccgag agcccgcggg
     1861 tcaccctgac gatcggcctc ggcgacttcc tgcgcctcgt caccaacagg ctgaacggca
     1921 tgcaggcgtt catgaccggc aagctcaagg tgtccgggga cctgttcttc gcgcagacct
     1981 tcgcgtcctg gttcgagcgc ccgtcgtcgt agcggccgga tcgggcaggt cgagttccac
     2041 ctcgaccacg accgcccgat ggtcggaggc cgggccggcc tggacgcggc tgcggcgcag
     2101 gcgcacgccg gggccgctcg cgagcacgtg gtcgagctgc acctcgggcc ggaccgtggc
     2161 gggcgggcgg ttgggccagg tcctgccccg cgccagcggt ctccagccgg gcagggagac
     2221 ggcgcggacg agcggcagcc acaggttcag gtcgccgagc agcaggcggg gcggcggcca
     2281 ggcggccagg tgccgctgga gggcgcgcag ctggccggcg ttcgacccgg gcacgaaggt
     2341 caggtgcgtc gcggccacgg tgaggggagt ggggcgcccg gccgcggcga cgagcgcgac
     2401 ccgcggctcg tcccggccgg cccgcggcag cctgacggtc tcctcccggc cgagcggcag
     2461 gcgcgacagc agcgcgaccc cgtaggcggc gccgccgggg tccggcgccc ctggcggcgg
     2521 tggctcggcc gcgccgcccg gcgtgccgga gagcgccggt gcgaagcgcc accccatccc
     2581 gagcccgtcg gcgagcacgc gcgcctggtc ggccctgccg ctgcggtcct ggtcgcggtc
     2641 gacctcctgg agggcgacca cgtcggcgtc cagggcgcgg agggtggcga gggtcaggtc
     2701 gaggtcgacc cggtcgccct ccctcgtcct gccgctgaag atgttccacg tcgccaggcg
     2761 cagcttcgtc acgccgggca tgctacccgc gctcgatccg gacgcgggcg gcgcggccgt
     2821 cgagctcgag cgtcgcgacc tggttctcga accgcggccc gcgcacgacg cgccacctga
     2881 ccgagggcgc cggcacgccg gccaggcggg cgatggcgcg ccaggcccat gcgaccggcc
     2941 gcgccatcgc gagccgcgag gccaggcgca gccgcgcggt gagcgggttg cgcagcggcg
     3001 agcaggtggg gccggtcccc ggcaggcgac cgggccagcc ggagcgcaag ggctcgcagc
     3061 gcgtcgacgc cgaggcgctg cccgtccggg aaccgcctcc accggggctc ggtgggtgcc
     3121 gccacccggc acgagccgaa cgcgagccgc agcggccgcc ccccgccgag cgtgcgcagg
     3181 acgctcggcg ggaaccggga gcccggctcg ggccaggccg ggtcgccgtc cagccgcacc
     3241 tcgtacccgt aggcgcggcc cggctcgagc ccgtccagca ccacgagcgc gtagtggtgc
     3301 ccctggacgg cgaacgtgcg ggcgcgccgg ctgagcacct ccgcctcgca cgccgcgctc
     3361 gtctccagcc agacggtggc ctcggtcgga ccgacgtacc gcagcatggg gccgaggacc
     3421 atggccggcg ctgtcgctgc atcgctcatg gcgccggatg gtagcaacgg gaccgcccgc
     3481 gtccctcggt cgacgccggg ctcggggagc cgggccgcgg ttgcctcctg gccgggagcc
     3541 ggccacactg ccggcatggc gaccacgctc ttcctcgtca ggcacgcgaa ggcggccgac
     3601 cgcgcgggct ggagcggcgc cgaccgggac cgcccgctga ccggcagggg ccgggcacag
     3661 gccgagcggc tggccctggc gctcgcgccc gagcggcccg tgctggttgc ggccagcccc
     3721 tggctccgct gcgtgcagac ggccgtgccg ctggccgaag cgctcggcgc cgagccgcgg
     3781 gccgacggcc ggctcggcta cgaccagccg gacatggccg cctgggtgcg ggacgcggcc
     3841 atgcacccgg gccgggccgc cgtcgcggtc gggcatggcg acctcatccc catgtacctg
     3901 ctgcgggccg gggtgctgga cggcctgccc gacttccgga ccgggtcgct gttccgggtc
     3961 cgggtcgagg acggccggcc ggtcgaggcc gtctacgtcg accggcgcga cctccacggt
     4021 cggggggccg tcccggagcg cccctgaggc gccggggccg ctcctcggaa ccggttcgcg
     4081 ccaccggccg ggtccccgta ggatgccccg accgctccca cttgacctgg ttcgaggaca
     4141 ccatgcacga gattctcctg cggcgccccg acgcgctggc cgccgagctc gcgagcttcg
     4201 cctgggaggt ggccgcccgt gggtgacctc cagcgcgtcg gcgtgatggg catcaggggg
     4261 tcgttctccg aggaggcggc cctcgagtac gcgcgcgaca acaagctcgg cgacgtcaag
     4321 atcgtcgagc tgctctccac cgaccgggtg ctccgggcgg tcgcggtcgg cgaggtcgac
     4381 ctcggcatct tcgccctgga gaactccaac ggcggggtcg tctacgagag cgtctacgcg
     4441 atggccgccc accgcttcaa ggtcctcgac ctgttcgaga tcgacgtcaa ccatgccctg
     4501 ctcggccacc cgagcctgcc t
//
LOCUS       DASPYY010000640         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_809358, whole genome shotgun sequence.
ACCESSION   DASPYY010000640 DASPYY010000000
VERSION     DASPYY010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809358"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VEP73_10060"
     CDS             <1..703
                     /locus_tag="VEP73_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT26813.1"
                     /translation="VGARILARLGYGVDVAVDGREAVAAVARREYGAVLMDCRMPEMD
                     GYLATAEIRRREPASRRLPVIAMTASACEEVRERCLAAGMDDCVAKPIMVADLEAVLA
                     RWVPDRATAPPAAGPAEAPEPAPAPGPAEASESALRTSLAGALLAGAPADLADLRAAV
                     GRGDARAVRFGAHRLKGATATLGVDGMAALCDELETLARAGELTAAGDLLGRLEQELD
                     HARTALDANAPKTPR"
     gene            760..1182
                     /locus_tag="VEP73_10065"
     CDS             760..1182
                     /locus_tag="VEP73_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYT26814.1"
                     /translation="MEDRGARGDPGHARWLVDGMNLIGSRPDRWWNDPDRAVRRLIEE
                     LGRYAAATGEDVTVVFDRQPPDVLPGAHGAAQVAFASRRGRNAADDEIVRMVAEDRSP
                     GSLRVVTSDRRLLERARELGAGVTSSASFRRRVDEALP"
     gene            complement(1184..2074)
                     /locus_tag="VEP73_10070"
     CDS             complement(1184..2074)
                     /locus_tag="VEP73_10070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT26815.1"
                     /translation="MDYGRPVEFGYFPEPAVAGVPRLFDEVALAERVGLDLVGIQDHP
                     YQWRYLDTFTLLANLAARTTRVRLFPDVANLPLRPPAMLAKAAASIDVLSGGRFEVGL
                     GAGGFWDAIEAMGGPRRAPGEAVEAFEDAIAIMRLMWSGERSVRYEGRHYRVRGVHPG
                     PPPPHPIGIWVGASGPRMLGLIGRLADGWLPSSPYVPPGELPGRQARIDEAAVAAGRE
                     PAAIRRAYNVSGRITARGGGFLDGPPEQWVEELAELVLERGMDTFILWAADPVAEQLE
                     RFAGEVVPAVRDAVAAARGR"
     gene            complement(2130..2909)
                     /locus_tag="VEP73_10075"
     CDS             complement(2130..2909)
                     /locus_tag="VEP73_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT26816.1"
                     /translation="MTLADHFEQVASVYESLRTTDEAPVREIRQLLPDRPVVGVDVGC
                     GTGRYSKLLRALLPEGSLLVGSDVSPAMLAELRAGNQGRALVAPVRSTAEELPLRTGS
                     LDLVTSFNSVHHFDLGRFLAGTVRVLRPDGLLFVYSRTPEQNARTIWGRYFPGFAERE
                     QRLHGERALRAAVRQTRGLVVVGTRTFRHARTSTRERLRAQAEGRHYSTFSLYAPDEL
                     PAAIEEFLARLPGGEVSWVDEHLLLVARRGRGTGRGGSSPR"
     gene            3127..3702
                     /locus_tag="VEP73_10080"
     CDS             3127..3702
                     /locus_tag="VEP73_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26817.1"
                     /translation="MNGVRIRRSAVVLALVAVVGGCSGGAVTLPSTGQPGPAGPVPPR
                     YLPRRDLTPGGTNPKVTQANIRQTICVPGWSARQRPSRTRSATLKRRVMRLYGYQGQP
                     PSRYEGDHLVPISLGGDPETNGRTENYWPEPWAGPYGARTKDDYEHWAYEQVCAGRMP
                     LVEAQHAFATNWYAGWVRAGRPGGGSGGGSS"
     gene            3839..4204
                     /locus_tag="VEP73_10085"
     CDS             3839..4204
                     /locus_tag="VEP73_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT26818.1"
                     /translation="MGGNGEHERAAQPEDLGRFFVERANAGDVDGLVALYEPDAVLAF
                     PAGQVTTGSQAVRQVYERLVAGRPRFAAGGQRPALRNGDLALTSTRLAGGGATVEVAR
                     RQPDGTWLWVIDQPNVLGE"
     gene            4504..4815
                     /locus_tag="VEP73_10090"
     CDS             4504..4815
                     /locus_tag="VEP73_10090"
                     /inference="COORDINATES: protein motif:HMM:NF039700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6247 family protein"
                     /protein_id="HYT26819.1"
                     /translation="MGCTYAQAISSPRDLDLSHEQFKVLLDALNEEGRENFTSDLVKA
                     IEAAQARNNLRPVKEVLEAWYRTLVVRQHPDYQRNLKWARESEDREVQTVDDALEGLE
                     S"
BASE COUNT          680 a         1688 c         1887 g          587 t
ORIGIN      
        1 cgtgggggcc cgcatcctgg ccaggctcgg gtacggcgtc gacgtggccg tcgacggccg
       61 ggaggcggtg gcggcggtgg cgcggcggga gtacggggcc gtgctgatgg actgccgcat
      121 gccggagatg gacggctacc tggccacggc cgagatccgc cgccgcgagc cggcgtcccg
      181 gcgcctcccg gtcatcgcca tgaccgcctc ggcgtgcgag gaggtgaggg agcggtgcct
      241 ggcggccggg atggacgact gcgtcgccaa gccgatcatg gtggccgacc tcgaggcggt
      301 gctcgctcgc tgggtgccag accgggcgac ggccccgccc gccgcgggac cggcggaggc
      361 gccggagccg gctccggccc cggggccggc ggaggcgtcg gaatcggccc tgcgcaccag
      421 cctggccggt gccctactgg cgggcgcgcc cgccgacctc gccgaccttc gcgccgccgt
      481 cgggcgcggg gacgcgcggg cggtgcggtt cggtgcgcac cgcctcaagg gggccacggc
      541 gacgctcggg gtcgacggca tggccgccct ctgcgacgag ctcgagacgc tcgcccgcgc
      601 cggcgagctc acggccgccg gcgacctcct cggccgcctg gagcaggagc tcgaccacgc
      661 caggaccgcc ctcgacgcga acgcgcccaa gacgccgagg tgacggcgcc cgccacggcg
      721 tctgccgcga tgccgggacg gcccgggtat gatcgccgga tggaggaccg tggtgcgcgc
      781 ggtgaccccg ggcacgctcg ctggctggtc gacggcatga acctgatcgg ctcccgaccg
      841 gaccggtggt ggaacgaccc ggaccgagcc gttcggcggc tgatcgagga gctgggccgc
      901 tacgccgccg ccacgggcga ggacgtgacg gtcgtcttcg accggcagcc tccggacgtc
      961 cttcccgggg cgcacggggc ggcccaggtg gcgttcgcct cgaggcgtgg gaggaacgcc
     1021 gccgacgacg agatcgtgcg gatggtggcg gaggaccggt ccccggggtc cttgagggtc
     1081 gtgacctcag acaggcgact gctggagcgg gccagggagc tgggcgccgg ggtgacgtcc
     1141 tcggcctcgt tccggcgccg ggtcgacgag gcgctcccgt agctcacctg ccgcgggccg
     1201 cggcgacggc gtccctgacg gcggggacga cctcgccggc gaagcgctcg agctgctcgg
     1261 cgaccgggtc cgccgcccac aggatgaacg tgtccatgcc gcgctcgagc acgagctcgg
     1321 cgagctcctc gacccactgc tccggcgggc cgtcgaggaa gccgccgccc cgcgcggtga
     1381 tgcggccgga gacgttgtag gcccgccgga tcgcggcggg ctcgcggccc gcggcgacgg
     1441 ccgcctcgtc gatcctcgcc tgacggcccg gcagctcccc gggcgggaca tacggcgacg
     1501 agggcagcca gccgtccgcc aggcggccga tgaggccgag catgcggggc ccggaggcac
     1561 cgacccagat gccgatcggg tgcgggggcg gcgggcccgg gtgcacgccg cggacgcggt
     1621 agtggcggcc ctcgtagcgc accgagcgct cgccgctcca catcaggcgc atgatcgcga
     1681 tcgcgtcctc gaaggcttcg acggcctcgc ccggagcgcg ccgaggcccg cccatcgctt
     1741 cgatcgcgtc ccagaagccg ccggcgccga ggcccacctc gaaccggccg ccgctcagca
     1801 cgtcgatcga ggcggcggcc ttggccagca tcgccggcgg ccgcaggggg aggttcgcga
     1861 cgtcggggaa gagccggacc cgggtcgtgc gcgccgccag gttcgcgagc agcgtgaagg
     1921 tgtcgaggta gcgccactgg taggggtggt cctggatgcc gaccaggtcg agccccaccc
     1981 gctcggcgag ggcgacctcg tcgaacaggc gggggacgcc cgccacggcc ggctcgggga
     2041 agtagccgaa ctccaccggc ctgccgtagt ccacgctcct cacctcccgt cagccgggga
     2101 tcctgccatg atccgcggct cccgcacgct cagcgaggag acgaccctcc gcgaccggtg
     2161 ccgcggccgc ggcgggcgac caggaggagg tgctcatcca cccaggagac ctcgccgcca
     2221 ggcaggcggg cgaggaactc ctcgatcgcc gccggcagct cgtcgggcgc gtagagggag
     2281 aacgtcgagt agtggcgccc ctcggcctgg gcccggagcc gttcgcgggt gctcgtgcgg
     2341 gcgtggcgga aggtgcgcgt gccgacgacc acgagtcccc tggtctggcg gacggcggcc
     2401 cggagcgccc gctcgccgtg caggcgctgc tcgcgctcgg cgaagccggg gaagtagcgg
     2461 ccccagatcg tgcgcgcgtt ctgctccggg gtgcggctgt agacgaagag caggccgtcc
     2521 ggccgcagca cgcggacggt gccggccagg aagcggccga ggtcgaagtg gtgcaccgag
     2581 ttgaacgagg tgaccaggtc gaggctgccg gtgcgcagcg gcagctcctc ggcggtcgag
     2641 cgcaccggcg ccacaagggc ccggccctgg ttgccggcgc ggagctcggc cagcatggcc
     2701 ggcgagacgt cgctcccgac gagcagcgac ccttcgggca ggagggcgcg caggagcttg
     2761 gagtacctcc cggtcccgca gccgacgtcg acgccgacga ccggacggtc gggcaggagc
     2821 tggcggatct cccgcaccgg cgcctcgtcg gtcgtgcgca acgactcgta gacggaggcg
     2881 acctgctcaa agtggtcggc cagggtcacg ttctacctcc tgggtgctcg ggggcatggc
     2941 ggcgcggctc cgggtcgttg cgcatatcat gcgctcgcct tgttgcgttc acacatcgct
     3001 cgccttgcgg ccatctcggc caggtgctcg ccaagcagca ccggccgagg cgtgcgcgcc
     3061 gcccttggag ggtagatccc acgtcgacca gtcgcgagcg ggtcggtctg cgacaccgag
     3121 gaggacgtga acggcgtgcg catccggagg tccgcggtgg tgctggcgct cgtggccgtg
     3181 gtgggcggtt gcagcggcgg cgcggtcacg ctgccgtcga ccgggcagcc gggcccggcg
     3241 ggcccggtcc cgccgcgcta cctgccccgc cgcgacctga cccccggcgg caccaacccg
     3301 aaggtcaccc aggccaacat acggcagacg atctgcgtgc ccggctggtc ggcgaggcag
     3361 cgcccgtcgc ggaccaggag cgcgaccctg aagcggcggg tgatgcgcct gtacggctac
     3421 caggggcagc cgccctcgcg gtacgagggc gaccacctgg tgccgatctc gctcggcggc
     3481 gaccccgaga ccaacgggcg gaccgagaac tactggcccg aaccgtgggc ggggccgtac
     3541 ggggcgcgca ccaaggacga ctacgagcac tgggcgtacg agcaggtgtg cgccgggcgc
     3601 atgcccctgg tcgaggcgca gcacgcgttc gcgaccaact ggtacgccgg gtgggtcagg
     3661 gcgggccgcc ccggcggcgg ttcgggtggc ggcagcagct gacccgcggg ctgcgcccgg
     3721 cggcggtcgg tccgctggac gcgccgaagg gaacgagttg tccgtcgttg tcgcactccc
     3781 gggagacgat cgcctgtacg ccgggggcga ctcctggcct tgacaggagg tgcgaacgat
     3841 gggcggcaac ggagagcacg agcgagccgc acagcccgag gaccttggcc ggttcttcgt
     3901 ggagagagcc aacgcgggcg atgtcgacgg gctggtggcg ctctatgaac ccgacgctgt
     3961 gctggccttc ccggccggcc aggtcaccac cggcagccag gcggtccgtc aggtgtacga
     4021 gcgactggtc gccggcaggc ccaggttcgc ggctgggggg cagcggcctg cgctccgcaa
     4081 tggcgacctg gcgctcacct cgacgcggtt ggccggtggc ggggccacgg tcgaggtggc
     4141 tcgccgccag ccggatggca cctggctctg ggtgatcgac cagccgaacg tcctcggaga
     4201 gtgagtcgcc cggccgggct ctggcagcgg atcccgggca ggtgtgcgta cggcctctca
     4261 cggaggcgaa gctccgcgcg aagtgggacg acccggcgac gtcggtcgac tggcgccggc
     4321 aggtcgtcat gatggccttc gagcgcgtcg aggtggcgag cgcgaagcgg gtcggccggc
     4381 gctcgccgac cgggtggacc aagtggaagg cgtacccacg agaagtcccg attgacagca
     4441 cgcacgtgca tacatgggta gatacttcag caggcaagcc atccccggga ggaggcggca
     4501 gcggtgggtt gcacgtacgc ccaggcgatc agtagcccga gggacctcga cctctcgcac
     4561 gagcaattca aggtgctgct cgacgcctta aacgaggagg gccgggagaa cttcacgagt
     4621 gacctcgtca aggcgatcga ggcggcgcag gcgagaaaca acctgcggcc cgtcaaggag
     4681 gtcctcgaag cgtggtaccg gacactcgtc gtccgccagc accccgacta ccagcgcaac
     4741 ctcaaatggg cgcgcgagag cgaggaccgc gaggtccaga cggtcgacga tgcactcgaa
     4801 gggctcgagt cataggtggc ctatcacgtc cagtggggac ca
//
LOCUS       DASPYY010000641         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1512958, whole genome shotgun sequence.
ACCESSION   DASPYY010000641 DASPYY010000000
VERSION     DASPYY010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512958"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="panC"
                     /locus_tag="VEP73_10095"
     CDS             <1..927
                     /gene="panC"
                     /locus_tag="VEP73_10095"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYT26820.1"
                     /translation="MTGTRSRASGSSCCAGWPGVRVVATSAELAAATAAARAAGASVG
                     FQPTMGALHAGHRANFAAARRENDVVVGSIFVNPLQFGPTEDLARYPRDLAGDLAACE
                     AEGVDVVFTPDEREVWPVRPAVRVCVGAMADRLEGALRPGHLDGVATVVAKLLHLVGP
                     ARAYFGEKDAQQLAVVRRMVADLGFPNQVVACPTVREPDGLATSSRNAYLSAAERRAA
                     PVLYRALQTGRSAWLAGAREPAAVERAAGELLAQAPEVEVQYVALVDPDSFEPVAEAA
                     AGQVLAVAARLGATRLIDNLTLRPASSPGGVD"
     gene            965..1807
                     /locus_tag="VEP73_10100"
     CDS             965..1807
                     /locus_tag="VEP73_10100"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYT26821.1"
                     /translation="MLLAIDVGNTETTIGVLDGEELVQHWRAATRAERTADEHALMLA
                     GFLAHQGLTFPGSLSGAAISSVVPRLTQALREMVGRYCPFDPLLVEPGVRTGLSIMTD
                     NPREVGADRVVNAVAAFALYGGPTVVVDFGTATTVDAISARGELLGNAIAPGIQISMN
                     ALAESAAQLRRVELVVPRSVVGKNTVESLQAGIVFGFAGQVDGLVRRMVAELGGAATV
                     VATGGPAGAVLDACETIQHYDSWLTLHGLRRIFDRNAAPKSATTVEEAGRDVRQGRAG
                     DGRG"
     gene            1800..>2258
                     /locus_tag="VEP73_10105"
     CDS             1800..>2258
                     /locus_tag="VEP73_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26822.1"
                     /translation="MARPVRPDRIEGRPLSPRAFRPFGIEAVVLGPPTGQEGLVRREG
                     VAVWRRLAEHAPVFDRAAAEAGARVGEGGVLPVRVVVAATPSRDRAMRVPALGERAAR
                     ASELAGGPPPATGWLLLVDEGPGETWLYLADEDDPAGALDGFLGRRGRTDF"
BASE COUNT          255 a          824 c          901 g          278 t
ORIGIN      
        1 atgacgggaa cgaggagtcg cgcgagcggc tcgagctgct gcgcagggtg gcccggcgtg
       61 cgcgtggtcg cgaccagcgc tgagctggcc gcggcaacgg cggcggccag ggcggcgggc
      121 gcgagcgtcg gcttccagcc caccatgggc gccctccacg ccggccaccg ggcgaacttc
      181 gccgccgcca ggcgggagaa cgacgtcgtg gtcggctcga tcttcgtgaa cccgctgcag
      241 ttcggcccga ccgaggacct ggcccgatac ccgcgggacc tcgccggcga cctggccgcg
      301 tgcgaggccg agggcgtcga cgtggtgttc acgccggacg agcgggaggt gtggccggtg
      361 cggccggcgg tcagggtctg cgtgggcgcc atggccgacc ggctggaggg cgccctgcgc
      421 cctggccacc tggacggggt ggccaccgtg gtcgcgaagc tcctgcacct ggtcgggccg
      481 gcccgggcct acttcggcga gaaggacgca cagcagctcg cggtggtccg ccggatggtg
      541 gccgacctcg gcttcccgaa ccaggtcgtc gcgtgcccga ccgtgcgcga gccggacggc
      601 ctggcgacgt cctcgcgcaa cgcctacctg tcggccgcgg agcgccgtgc cgcgcccgtg
      661 ctgtaccggg cgctccagac gggccggtca gcctggctcg ccggcgcgcg ggagcccgcc
      721 gcggtggaga gggcagccgg cgagctgctc gcccaggccc cggaggtcga ggtccagtac
      781 gtggccctgg tcgaccctga cagcttcgag ccggtcgcgg aggcggcggc cgggcaggtg
      841 ctcgcggtcg cggcccggct cggcgccacc cgcctgatcg acaacctgac tctccgtccg
      901 gcgagctcgc cgggcggtgt tgactgaaca tgacccgcgc ggcgcgggga cgggagggac
      961 gagcgtgctg ctcgcgatcg acgtcggcaa caccgagacc accatcgggg tgctcgacgg
     1021 ggaggagctg gtgcagcact ggcgcgcggc caccagggcc gagcggacgg cggacgagca
     1081 cgcgctcatg ctggccggct tcctcgccca ccaggggctc acgttcccgg ggtcgctgtc
     1141 cggggcggcc atctcctcgg tggtgccgag gctgacccag gccctccgcg agatggtggg
     1201 gcgctactgc ccgttcgacc cgctgctggt cgagccgggc gtgcgcaccg ggctgtcgat
     1261 catgaccgac aacccgcgcg aggtgggcgc cgaccgggtg gtcaacgcgg tcgcggcctt
     1321 cgccctctac ggcggcccca ccgtcgtggt cgacttcggc accgccacca ccgtcgacgc
     1381 gatctcggcg cggggggagc tgctcggcaa cgcgatcgcc cctggcatcc agatctcgat
     1441 gaacgccctt gccgagagcg cggcgcagct ccgccgggtc gagctcgtcg tgccgaggtc
     1501 ggtggtcggc aagaacaccg tggagagcct gcaggcgggc atcgtgttcg gcttcgccgg
     1561 ccaggtggac gggctggtcc gccggatggt ggccgagctc ggcggcgcgg ccaccgtggt
     1621 cgccaccggc gggccggccg gcgcggtgct cgacgcctgc gagacgatcc agcactacga
     1681 ctcctggctg acgctccacg ggctgcgccg catcttcgac cgcaacgcgg cgccgaagtc
     1741 ggccacgacg gtcgaggagg ccggccgcga cgtgcgccag ggccgggccg gggacggccg
     1801 tggctaggcc ggtccggccc gaccgcatcg aggggcggcc gctctcgccg cgcgcgttcc
     1861 gcccgttcgg catcgaggcg gtcgtcctcg ggccgcccac agggcaggag gggctggtgc
     1921 gtcgggaggg ggtcgcggtc tggcgccggc tggcggagca cgccccggtg ttcgaccggg
     1981 ccgccgccga ggccggcgcg cgggtcggcg agggcggcgt gctgccggtg cgggtggtcg
     2041 tggccgccac gcccagccgg gaccgggcca tgcgcgtccc cgccctcggc gagcgtgccg
     2101 cccgcgcctc cgagctggcc ggggggccac cgccggccac cggctggctg ctcctcgtcg
     2161 acgaggggcc gggggagacc tggctctacc tggccgacga ggacgacccg gcgggcgccc
     2221 tcgacggctt cctcggccgc cgcggccgga ccgacttc
//
LOCUS       DASPYY010000642         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_809535, whole genome shotgun sequence.
ACCESSION   DASPYY010000642 DASPYY010000000
VERSION     DASPYY010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809535"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VEP73_10110"
     CDS             <1..1782
                     /locus_tag="VEP73_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYT26823.1"
                     /translation="ALGQATTAKSELNASRDLLARAGGFSAGSLPERLSLVDRAAIHA
                     ENARLELAGGPVRLLGLLPFLGRDVRVAQAVAATAGETAQATRQVVTALEPVEHAPPS
                     AASLDRASTALLGLHEVLDRGLHDVGRTRPLVASRGARAEFLAAAGSADRTAFQAGEG
                     LRLAARLYGPAGTARWFLAFQNPAELRGTGGLIGEYGILESSPGGPRMTHVAPYGELD
                     ARVKDGVDLPAGVAQRYDRFPVGSAFWAVNIPPDLPTVGGTITRLYQQVTGTRVDGVI
                     AIDPLAVAEILKVGGPITVAGNRLDGANVARETLVRAYVRFAQDNLARQRYLQEIARQ
                     TLAVFQRAVRAHPVELVRGLATAARGRHLQLYSSDPDGQRALLDLGIAGSAAAPTDNS
                     DYLMPVGVNTGGNKMDAFLRRRIGYRVKLLPDGGARAVASVTLGNAGPPPGLPRYVVG
                     PFDARFRPGENSQFQTLYVAGSYGFAAATVNGRPVRADAEAELGGLALSQSLDVPARS
                     SATVTYSLVRSHAVEVVDRTHLRYRLLLRPQATAWPDQVAVSVTPPPGWRFGALPAGF
                     HPNGQAAAWSGPLDREQALAFDLVPVR"
     gene            1998..3011
                     /locus_tag="VEP73_10115"
     CDS             1998..3011
                     /locus_tag="VEP73_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26824.1"
                     /translation="MRKVLSAVAIAVALTTVSAPAFADPSLNVTPSKVRPGDDISVSG
                     RCPSHRGGTVEITLAGKHVVKVRADGKGRFSGEGTVPDVKPGKREVRAVRGRETCGDK
                     DITVLGRAPVKFSVVPRRVERGGVVTISGGGCKPGSKVTFYLDGRKIGAKTSSRRDGS
                     FHDTATIPTGTSLGKHTITAVCNGKVVGSAKIRVVEEYPGTGGRSSTLAVSRSTVAAG
                     TTVSLVTTPCPTGTADAALNDTKLVLASPARSGGLFSTTIPKGTPAGTYTLAARCDGR
                     VAGVATVRVTPAAEALPAQATGLPSTRANIVPGLAAGVALIAAGALTLLRIRRRRGAR
                     MVW"
     gene            3313..>3651
                     /locus_tag="VEP73_10120"
     CDS             3313..>3651
                     /locus_tag="VEP73_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26825.1"
                     /translation="MRKALPALGVAVALTLFGGPADAAPSLKVSPGVVRVGGKVKVAG
                     RCPRNSSRNVTIEVAGRVIIQTRANRNRGFSSKGRVPNAPGAREVRAECGGKLAGTGK
                     ITILRKASFSV"
BASE COUNT          483 a         1417 c         1297 g          454 t
ORIGIN      
        1 gcgctcggcc aggcgaccac ggcgaagtcg gagctgaacg cgtcgcggga cctgctcgcg
       61 cgcgcgggcg gcttcagcgc cgggtcgctg cccgagcgcc tctccctcgt cgaccgggcg
      121 gccatccacg ccgagaacgc ccgcctcgag ctggcgggcg ggccggtccg cctcctcggc
      181 ctgctgccgt tcctcggccg cgacgtgcgc gtcgcccagg cggtggccgc caccgccggc
      241 gagaccgcgc aggcgaccag gcaggtggtc accgcgctcg agccggtcga gcacgcgcca
      301 ccgagcgcgg cgagcctgga ccgggcgtcg accgccctgc tcggcctcca cgaggtgctc
      361 gaccgcgggc tccacgacgt cgggcggacc aggccgctcg ttgccagccg cggcgcccgc
      421 gccgagttcc tggccgccgc cgggtccgcc gaccgcaccg cgttccaggc cggcgagggg
      481 ctgaggctgg ccgcgcgcct ctacggcccg gccgggacgg cccgctggtt cctcgccttc
      541 cagaacccgg ccgagctgcg cggcaccggc ggcctgatcg gcgagtacgg catcctcgag
      601 tccagccccg gcgggccccg catgacccac gtcgcgccct acggggagct cgacgcgcgg
      661 gtgaaggacg gcgtggacct gcccgccggg gtcgcccagc gctacgaccg cttcccggtc
      721 ggctcggcct tctgggcggt caacatcccc cccgacctgc ccaccgtggg cgggaccatc
      781 acgaggctct accagcaggt caccggcacg cgggtcgacg gcgtgatcgc catcgacccc
      841 ctggccgtcg ccgagatcct caaggtcggc ggcccgatca cggtcgccgg caaccggctg
      901 gacggcgcca acgtcgcccg cgagaccctg gtgcgggcct acgtccgctt cgcgcaggac
      961 aacctggcca ggcagcgcta cctgcaggag atcgccaggc agaccctggc cgtcttccag
     1021 cgggccgtgc gcgcccaccc ggtcgagctg gtcagggggc tggccaccgc cgcgcgcggc
     1081 cggcacctgc agctgtactc gagcgacccg gacggccagc gggccctgct cgacctcggc
     1141 atcgccggca gcgcggcggc gccgaccgac aacagcgact acctcatgcc ggtcggcgtc
     1201 aacaccggcg gcaacaagat ggacgccttc ctgcgccgca ggatcggcta ccgggtgaag
     1261 ctgctgcccg acggcggtgc cagggcggtg gcatcggtca ccctgggcaa cgccgggccg
     1321 ccgccgggcc tgccccgcta cgtcgtcggc ccgttcgacg cgcgcttccg gcccggcgag
     1381 aacagccaat tccagaccct ctacgtggcc ggctcgtacg gcttcgccgc ggcgaccgtc
     1441 aacggccggc cggtgcgcgc cgacgcagag gcggagctgg gcggcctcgc cctgtcgcag
     1501 tccctcgacg tgcccgcccg cagctcggcc acggtcacct acagcctggt ccgcagccac
     1561 gcggtcgagg tcgtcgaccg cacccatctg cgctaccggc tcctgctccg gccgcaggcg
     1621 accgcctggc ccgaccaggt cgcggtctcg gtgaccccgc cgcccggctg gcgcttcggc
     1681 gccctgcccg cagggttcca tccgaacggt caggcggccg cctggtccgg cccgctcgac
     1741 cgggagcagg ccctggcctt cgacctcgtg ccagtgcggt agccgccgtg tcggctaccc
     1801 ctcgccgcct gtcccccgct cggacaggcc gcatggccct atcggatcgt tcgagagttt
     1861 cgtccttgtc tggatcgacc tgcgcccgta gcttcacgac ccgccgggga aggtgatccc
     1921 ggcgatcgac aggcacgtcc ggagccttcg gctgcgggcg tgccgggggg gcagcaccaa
     1981 ccacaaggag gaaccacatg cggaaggtcc tttccgcagt cgccatcgca gtcgctctga
     2041 cgacggtcag cgcgcccgcc ttcgcggacc cgtcgctgaa cgtcacgccc agcaaggtcc
     2101 gcccgggcga cgacatctcc gtctccgggc gctgcccgtc gcacaggggc ggcaccgtcg
     2161 agatcacgct ggccggcaag cacgtcgtca aggtgcgggc ggacgggaag ggccggttct
     2221 ccggcgaggg caccgtcccc gacgtgaagc ccggcaagcg cgaggtccgc gccgtacgcg
     2281 gcagggagac ctgcggcgac aaggacatca ccgtcctcgg ccgcgcgccg gtcaagttct
     2341 ccgtcgttcc ccgccgggtc gagcggggcg gcgtggtgac gatctcgggc ggcggctgca
     2401 agccggggtc gaaggtcacc ttctacctgg acggcaggaa gatcggggcg aagacgtcgt
     2461 cgaggaggga cgggagcttc cacgacaccg ccacgatccc gaccgggacc tccctcggca
     2521 agcacaccat caccgccgtc tgcaacggca aggtcgtcgg ctcggccaag atccgcgtgg
     2581 tggaggagta cccggggacg ggcgggcgct cgagcacgct cgcggtctcg cggtcgaccg
     2641 tcgccgccgg cacgacggtg tccctggtga ccacgccgtg cccgaccggc acggcggacg
     2701 ccgcgctgaa cgacaccaag ctcgtgctcg cgtccccggc ccgctccggc ggcctgttca
     2761 gcacgacgat cccgaagggc acgcccgccg gcacctacac gctggccgcg cgctgcgacg
     2821 gccgggtcgc cggggtcgcg accgtgcgcg tcaccccggc cgccgaggcg ctccccgccc
     2881 aggcgaccgg ccttccgtcg acacgcgcga acatcgtgcc cggcctggcg gccggcgtcg
     2941 cgctcatcgc cgccggtgcg ctgacgctcc tgcgcatcag gcggcgccgc ggggcccgga
     3001 tggtgtggta gcgcgccccg caggccccgc cgtaccgcgc ctggccggcg gcctccgggt
     3061 ccgccggcca ggcggtgccg gtcgggcctg ctctcccgcg cggcgccgcg acaaccctcc
     3121 cacaagcaca gcaaccgccc tggctggcat cttgatccga tcgagctgtt aaggaagttc
     3181 gtcttgttgc gacggggcgc cctcggttag gttgccgagc cgctggcaca gcaccgcagc
     3241 gatcggaagg cgcacggacg cgacctgcgt ccggcgcgct ccggggcaag ccccgatcaa
     3301 aggaggaagt gcatgcgcaa ggcgctccca gcgcttggcg tcgcagtcgc gttgacgctc
     3361 ttcggcggcc ccgcggacgc ggccccgtcg ctcaaggtct caccaggcgt ggtccgcgtc
     3421 ggcggcaagg tcaaggtcgc cggcaggtgc cccaggaaca gctcccgcaa cgtcacgatc
     3481 gaggtggccg gcagggtcat catccagacg agggccaacc ggaaccgcgg gttctcgtcg
     3541 aagggcaggg tgccgaacgc cccgggagcg cgcgaggtgc gcgccgagtg cggcggcaag
     3601 ctggccggca ccgggaagat caccatcctc aggaaggcgt ccttctcggt g
//
LOCUS       DASPYY010000643         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1793182, whole genome shotgun sequence.
ACCESSION   DASPYY010000643 DASPYY010000000
VERSION     DASPYY010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793182"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEP73_10125"
     CDS             complement(<1..353)
                     /locus_tag="VEP73_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYT26826.1"
                     /translation="MLRAIVLGIVQGLTEFLPISSSAHLAIVPRLLGYRPPELSFEIL
                     LHFGTLAAVVAYFARDLWGFVLGVVAPSRLGAAEAAVKRRLLLLLVVASVPAAALGLI
                     LSNWADEQTKQPLRAS"
     gene            complement(435..1061)
                     /locus_tag="VEP73_10130"
     CDS             complement(435..1061)
                     /locus_tag="VEP73_10130"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HYT26827.1"
                     /translation="MPPRWPAAPGGRGRGRRPAGRQVAAGLGAVVLAVAAGVLAAARP
                     RPAPVGAPSGTAVLVAARDLPAGSVPAAVDLHLARLPADAVPADALAAGVPPGAPLVV
                     ALRRGDLVTRRDVGGGPVAPAAGLRGYALEVGDGVEAGPLVPGDRVDVLAANGGAVAT
                     VLATVPVLRVVPGDQGRAAMIVLGVTAKGAETLAAARAAGSVTLIQAP"
     gene            complement(1063..1872)
                     /locus_tag="VEP73_10135"
     CDS             complement(1063..1872)
                     /locus_tag="VEP73_10135"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HYT26828.1"
                     /translation="MALPTAELGVFGGSGFYSFLDGVTEHAVSTPYGDPSDKVAVGEV
                     DGRTVAFLPRHGRRHQFPPHRINYRANLWAMKELGVTRVVGPCAAGSLQAEAAPGHFV
                     ICDQVVDRTWGRADTFYDGPETTHISFADPYCPELRELAVKKAEELGITVHDSGTMVV
                     IQGPRFSTRAESQWFQRAGWRVINMTGYPEAPLARELEICYVNVSLITDYDVGLEGLP
                     GVAPVSHAEVVRVFQENNARLRDLLHSLLPAVPGERSCPCATALTGARLEA"
     gene            complement(2029..2421)
                     /locus_tag="VEP73_10140"
     CDS             complement(2029..2421)
                     /locus_tag="VEP73_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYT26829.1"
                     /translation="MPTYEYACTECGNHVDVVQSMTDPPMTTCPVCGGPLRKVFAPIG
                     IVFKGSGFYRTDSRAKAGASDGAGKEKAGKEKAGKAGEAGGGDAKSSSTPGGRSSSSS
                     GSSSGSSSGSSSGSPSSGSSSSKPAKSA"
     gene            2608..>3149
                     /locus_tag="VEP73_10145"
     CDS             2608..>3149
                     /locus_tag="VEP73_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26830.1"
                     /translation="MATEGLGGLGLSLRSRLTLFFVVIVVVPVAATTFWQWRRSAAVA
                     VRYEREALAVDGHAAGLVLRAEAGQASGAARSLAGSKEVQAALAAPDRDALQAVLDRG
                     GSGDLLVAVTGAHGELLAHRGRTAPTLLAGVAVPRLETLLREAPEGTGGGLLQRQSVP
                     VHRRDCHGGHCLLGTVVAGV"
BASE COUNT          415 a         1118 c         1253 g          363 t
ORIGIN      
        1 gaggcccgca gcggctgctt cgtctgctcg tcggcccagt tcgagaggat caggccgagt
       61 gccgcggccg ggaccgacgc gaccacgagc aggaggagca gccggcgctt gaccgccgcc
      121 tcggccgctc ccagccggct cggcgccacc acgccgagca cgaagcccca caggtcgcgg
      181 gcgaagtagg cgaccactgc ggcgagcgtc ccgaagtgca gcaggatctc gaaggagagc
      241 tccggcgggc ggtagccgag cagccggggg acgatcgcga ggtgggcgga cgacgagatg
      301 ggcaggaact cggtgagtcc ctggacgatg ccgagcacga tcgcgcggag cacgtgacgc
      361 ctttccagtc gacgcggatg agcggatgac cggggaagta tggcagcccc gggcgcggca
      421 gggctcccgg cgggtcaggg ggcctggatg agggtgaccg acccggccgc gcgggcggcg
      481 gccagcgtct cggcgccctt ggcggtcacc ccgagcacga tcatggccgc ccggccctgg
      541 tcgccgggca ccacccgcag gacgggcacg gtggcgagga cggtcgccac cgcgccgccg
      601 ttcgcggcga gcacgtcgac ccggtcgcct ggcaccagag gtccggcctc gaccccgtcg
      661 ccgacctcca gggcgtaccc ccgcaggccg gcggccggag ccaccggccc gccgccgacg
      721 tcgcgccggg tgaccaggtc gccccggcgc agggcgacga cgagcggggc gccgggcggg
      781 acgcccgccg cgagggcgtc ggcggggacc gcgtcggccg gcagccgggc aaggtggagg
      841 tcgacggcgg ccgggaccga cccggcgggc aggtcgcggg cggcgacgag cacggccgtg
      901 ccgctcgggg cgccgacggg agccgggcgt ggccgcgcgg cggcgagcac gcccgcggcg
      961 acggcgagga cgacggcacc gagcccggcc gccacctgcc ggcccgccgg gcgccgcccg
     1021 cggccccggc ccccgggggc cgccggccat cgcggcggca cgtcaggcct cgaggcgcgc
     1081 tccggtgagc gcggtcgcgc acgggcagga gcgctcgccc ggcaccgcgg ggagcaggga
     1141 gtggagcagg tcccgcagcc tcgcgttgtt ctcctggaac acgcggacga cctcggcgtg
     1201 ggagaccggg gcgacgccgg gcagcccctc gaggccgacg tcgtagtcgg tgatcagcga
     1261 gacgttcacg tagcagatct cgagctcgcg ggcgagcggc gcctccgggt agccggtcat
     1321 gttgatgacc ctccagccgg cgcgctggaa ccactgggac tccgctctgg tggagaagcg
     1381 gggaccctgg atcacgacca tcgtgccgct gtcgtgcacg gtgatgccga gctcctcggc
     1441 cttcttcacc gcgagctcgc gcagctccgg gcagtagggg tcggcgaagg agatgtgcgt
     1501 cgtctcgggg ccgtcgtaga acgtgtcggc ccggccccag gtccggtcga ccacctggtc
     1561 gcagatgacg aagtggcccg gggcggcctc ggcctgcagg gagccggccg cgcacggccc
     1621 gacgacccgg gtcacgccga gctccttcat ggcccacagg ttggcgcggt agttgatccg
     1681 gtgcggcggg aactggtggc ggcggccgtg gcgggggagg aaggcgaccg tgcgcccgtc
     1741 cacctcgccg accgcgacct tgtcggacgg gtcgccgtag ggggtcgaga ccgcgtgctc
     1801 ggtcacgccg tcgaggaacg agtagaagcc ggagccgccg aagaccccga gctcagccgt
     1861 tggaagggcc atctcgtgca cacctcatcc tgtcggcggg gagccccgga gccggggagc
     1921 ccgcgtcgcc ccgccggcgc gggtccggcc gggggccgcc aggtcggcgg cagcgcgcat
     1981 ggtatcgaag gcccgcgcgc gggccgaagc cgtgtccaca ggggtgggct acgcgctctt
     2041 cgccggcttc gacgacgagg agccggacga cggggagcct gaagaggagc ctgaggagga
     2101 gccggaggag gagccggagg aggacgacga cctgccgccg ggcgtggagg acgacttggc
     2161 gtccccgccg cccgcctccc cggccttgcc ggccttctcc ttgccggcct tctccttgcc
     2221 ggcaccgtcc gatgcgcccg ccttggcgcg ggagtcggtg cggtagaacc ccgacccctt
     2281 gaacacgatg ccgatggggg cgaagacctt gcgcagcggg cccccgcaga cggggcaggt
     2341 cgtcatgggg gggtcggtca tggactgcac cacgtcgacg tggttgccgc actccgtgca
     2401 ggcgtactcg taggtcggca tcgaaactcc tggaaggaag acgagctggc caaccgaggc
     2461 caccgctcga tgcgcacagt gtaccaaccc gggctcgagc cctccggacg gcgcctccca
     2521 cgctcgggcg gccgtcgggg tagccgataa gcatttcgag agttattgac gcatcgaccg
     2581 attcaggatc aggatcgccg ggccgggatg gccaccgagg ggctgggggg cttgggcttg
     2641 tcgctgcgat ctcgcctgac gctgttcttc gtcgtcatcg tggtcgtgcc cgtggcggcg
     2701 acgacgttct ggcagtggcg gcgctcggcc gcggtcgccg tccgctacga gcgcgaggcg
     2761 ctcgcggtcg acgggcacgc cgcggggctg gtcctgcggg ccgaggccgg ccaggcgtcg
     2821 ggggccgcac ggtcgcttgc cggcagcaag gaggtccagg cggccctggc ggcgccggac
     2881 cgcgacgcgc tgcaggccgt cctcgaccgc ggcgggtccg gcgacctgct ggtcgccgtg
     2941 acgggggcgc acggcgagct gctcgcccac cggggccgga cggcgcccac cctgctggcc
     3001 ggcgtcgccg tgccgcggct cgagaccctg ctccgcgagg cgcccgaggg cacaggcggg
     3061 gggctcctgc agcgccagag cgtcccggtg caccgccgcg actgccacgg cggccactgc
     3121 ctgctcggca ccgtcgtggc cggcgtctg
//
LOCUS       DASPYY010000644         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1935061, whole genome shotgun sequence.
ACCESSION   DASPYY010000644 DASPYY010000000
VERSION     DASPYY010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935061"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VEP73_10150"
     CDS             complement(<1..213)
                     /locus_tag="VEP73_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYT26831.1"
                     /translation="MEEAPGRANGLVPGEAARRRLRLVVAYDGAPFSGFARQRGRRTV
                     QGELEAALSRLAREPVETVGAGRTDAG"
     gene            complement(218..934)
                     /gene="rplQ"
                     /locus_tag="VEP73_10155"
     CDS             complement(218..934)
                     /gene="rplQ"
                     /locus_tag="VEP73_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYT26832.1"
                     /translation="MPTPTKGPRLGGGPSHERMIISNLARSLFAEGRIRTTEAKAKRL
                     RPQAERLITFAKRGDVASRRQVLTVVRDKQVVHKLFAEIAPRFADRQGGYTRILKLGP
                     RPGDSAPMALIELVEQGAGGDDRAAAGAAAPRRRRFGRGRAAAQAGSPGAAAGANRAE
                     RRAAARRRSQEGDTALAGEQVDAEDAEERPAVPTDAEIEAGAPVAGDDGEAASEDEDE
                     VRAEEAPSAGDDEGEDTRRA"
     gene            complement(994..1926)
                     /locus_tag="VEP73_10160"
                     /pseudo
     CDS             complement(994..1926)
                     /locus_tag="VEP73_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            complement(2002..2628)
                     /gene="rpsD"
                     /locus_tag="VEP73_10165"
     CDS             complement(2002..2628)
                     /gene="rpsD"
                     /locus_tag="VEP73_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYT26833.1"
                     /translation="MARYTGPDCRLCRREKTKLFLKGSKCESPKCPVERRPYPPGEHG
                     RGRTKESEYLLQLREKQKARRIYGVLETQFHNYYQEASRRKGITGEVLLQLLETRLDN
                     VAYRGGLARSRDQARQLVRHSHVTVNGRKVNIPSYQVKAGDVVQVKEKMRGNLNVHES
                     IQLQTGRPQPGWLELNAGEAKLTVNSLPTRDQVEVPLQEQLIVELYSK"
BASE COUNT          304 a          977 c          995 g          461 t
ORIGIN      
        1 cccggcgtcg gtgcggccgg ccccgacggt ctcgaccggc tccctggcga gccgggagag
       61 cgcggcctcg agctcaccct gcacggtgcg ccgtccacgc tggcgcgcga acccggagaa
      121 cggggcgccg tcgtaggcga cgaccaggcg gagccggcgc ctggccgcct cgccgggaac
      181 gagcccgttg gcgcgccccg gggcctcctc catccggtca ggccctccgg gtgtcctcgc
      241 cctcgtcgtc gccggccgag ggcgcctcct cggcgcggac ctcgtcctcg tcctcgctcg
      301 cggcctcgcc gtcgtcaccg gccacaggcg cgccggcctc gatctcggcg tcggtgggca
      361 cggccggccg ctcctcggcg tcctcggcgt cgacctgctc gccggcgagg gcggtatcgc
      421 cctcctggga ccgccggcgc gcggcggcgc ggcgctcggc ccggttcgcg cccgcggcgg
      481 ctcccggcga gccggcctgg gcggctgccc ggccgcggcc gaagcgtcgg cggcgcggcg
      541 cggcggcgcc ggcggcggcc cggtcgtccc cgccggcgcc ctgctcgacc agctcgatca
      601 gggccatcgg ggccgagtcg cccggcctcg ggccgagctt gaggatgcgg gtgtagccgc
      661 cctgccggtc ggcgaaccgc ggcgcgatct cggcgaacag cttgtggacg acctgcttgt
      721 cccgtacgac ggtgaggacc tggcgccggc tggcgacgtc gccccgcttc gcgaaggtga
      781 tgaggcgctc ggcctggggc cgcaggcgct tggccttggc ctcggtggtg cggatgcgcc
      841 cctcggcgaa cagcgaccgg gccaggttcg agatgatcat gcgctcgtgg ctcggccccc
      901 cgcccaggcg aggccccttg gtcggggtcg gcatcggttc tcttcctctc ttctctcggg
      961 tggctcgggc gggctactgg ctgctggcta ctcttcgcgg agcgacaggc cgagctcgtg
     1021 cagcttctcg cggacctcgt cgatcgactt ggcgccgaag ttgcggatgt cgagcaggtc
     1081 ggcctctgac ttggtgacga gctcgcccac ggtgacgatg ccctcgcgct tcaggcagtt
     1141 gtacgaccgc accgacaggt cgagctcctc gatcggggtg tcgaggttgg cgccgagcgg
     1201 cccggtctgc tcggccggcc cgagcgacag gccctcgccc ccgccggtca ggtccgcgaa
     1261 caggctgacc agctcgcgca gggtgccgcc ggccgaggcc agggcgtcct ccgggctcag
     1321 cgacccgtcg gtctccacgt cgaggagcag ccggtcgtag ttggtcatct cacccgagcg
     1381 ggtggcctcg acggtgtagc tgacccggcg gaccggggag aagatcgagt ccaccgggat
     1441 gcgaccgatc tcgtccgagg cggacttgtt gcgctcggcc ggccggtagc cgcgaccctt
     1501 ctccacccgc agctccatct cgagcttggc cttggagttc agggtggcga gccgcagctc
     1561 ggggttgatg acgtcgatgt cggcgggcgg cacgatgtcg gccgcggtca cctcggctgg
     1621 gccggtcgcc cgcaggaaca tcgtcgccgg ctcgttggtc tcgctgcgca ggcgcagctc
     1681 cttgacattc aggatgatgt cggtcacgtc ctccttcacg ccctggatgg acgagaactc
     1741 gtgcaggacg ccgtcgatct tgatcgaggt caccgcggcg cccgggatgg acgacaggag
     1801 cgtccggcgc agcgagttgc cgagcgtgta gccgaagccg ggctcgagcg gctcgatgac
     1861 gaagcgcgaa cggttgtcgg tcccgtccag cgggtgcgac tcgatgcggg gacgcgagat
     1921 ctccatgatg ctctgttcac ctccggctcc ccggcccgcc atatgactcg gggacgccct
     1981 ctgtgcctgg ttgcttctcg gttacttcga gtacagctcg acgatgagct gctcctgcag
     2041 cggcacctcg acctggtccc tggtcggcag cgagttgacc gtgagcttcg cctcgccggc
     2101 gttcagctcg agccagcccg gctggggccg gccggtctgg agctggatcg actcgtggac
     2161 gttgaggttg ccgcgcatct tctccttgac ctggaccacg tcccccgcct tcacctggta
     2221 cgaggggatg ttcaccttcc tgccgttgac cgtcacgtgg gagtggcgga cgagctgcct
     2281 cgcctggtcg cgcgagcggg ccaggccgcc ccggtaggcg acgttgtcca gccgggtctc
     2341 gaggagctgc agcaggacct cgccggtgat gcccttccgg cgggaggcct cctggtagta
     2401 gttgtggaac tgcgtctcga ggacgccgta gatgcggcgc gccttctgct tctcacggag
     2461 ctggagcagg tactcggact ccttggtccg gccccgcccg tgctcgcccg gtgggtacgg
     2521 ccgccgctcg accgggcact tcgggctctc gcacttggag cccttcagga acagcttggt
     2581 cttctcgcgg cggcacagcc tgcagtcggg acctgtgtat ctggccaatt tcgggctact
     2641 ccttctcggc tcccacggtc gggcgctcct cgccggcgcc caccgggcct gtcggccggg
     2701 ggtcgatgct ggtcgggtcg ggctgacggg tcagacg
//
LOCUS       DASPYY010000645         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1513229, whole genome shotgun sequence.
ACCESSION   DASPYY010000645 DASPYY010000000
VERSION     DASPYY010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513229"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1587
                     /locus_tag="VEP73_10170"
     CDS             94..1587
                     /locus_tag="VEP73_10170"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYT26834.1"
                     /translation="MLRVSLGKSDAAGAKADLLAVGVFENRVLGPGAEEAVAALETPL
                     EPLLDARGFTGKAGEVVVLPTLGRLPAATLLLVGLGERDKVDADVLRRAAAATVRHAG
                     GAKKAVTTLAQALPGDPALAARAVAEGAVLADYRFDKYKAKANGSDPQRRLSSLVVAP
                     GDGKKGDLAAAVAAGQARAAATNVARDLSNEPANNLHPADLADAARKLAGKGVTVKVK
                     DEKALAREGFGGIMGVGQGAESPPRLVEMTYRPEGARASVVLVGKGITFDSGGLSLKP
                     PDSMTAMKTDMSGAAAIVGVMSVLDDLGVKVAVTGYLASAENMPSGSATRPGDVLTMK
                     NGKTVEVLNTDAEGRLVMADALAVGAAARPDAIVDVATLTGACVIALGTRYTGLMSND
                     DALAAELLEAARDAGEPTWQLPLPEEYHKEVVSDVADLKNVGQRWGGALTAGLFLKEF
                     VDDRPWVHLDIAGPSRSDSEDGYIPKGSTGAAVRTLLSWLERRGSSS"
     gene            1669..2763
                     /locus_tag="VEP73_10175"
     CDS             1669..2763
                     /locus_tag="VEP73_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT26835.1"
                     /translation="MPSGPPPSRVGQKDWRQLRYFATAVEGLGPILAYEVGTLAGTEV
                     EPRRQFDGRNDVVSFSAKRPDGLAGLRTSEDVFVEVSAARRVGTLRALVGALRDDRAL
                     EQALSVYSAQVRPLRARMTFRVVARVLSEREFPRTALRDELSRVIQQARPRWRATDPA
                     ALELWALESTPGRIRLGVRLSGSAMRHRGGRNLERPGALRPTVAAAMVLLAGTPTVRA
                     SATLLDPCCGSGTIPAEAAAAGWIPLGADLDPGAVAVARANLGGTARLLLADVRRLPL
                     ADASVAALASNLPFARQYTVQGSPGAWFAAALAELDRVTQAPAAVVLLVPTTNAFQRA
                     LAGRPSLALERRLDLRLLGMRTALWQLRRR"
     gene            complement(2838..3362)
                     /locus_tag="VEP73_10180"
     CDS             complement(2838..3362)
                     /locus_tag="VEP73_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26836.1"
                     /translation="MDTDPIAGEVADWLAARLPPGWFTGPAEVAVDGEEILVVGRLTP
                     PALPPGASEAAVAAACLERAKEFREATREQRMRVAAEAEHRFARRVSWGADCGDRRTL
                     FTTLSVPVMTRLRLRERAVLDTLIGAGVARSRSEALAWCVRLVGTHQADWISDLRDAL
                     LAVKKVRAEGPTSR"
     gene            3454..>3933
                     /locus_tag="VEP73_10185"
     CDS             3454..>3933
                     /locus_tag="VEP73_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HYT26837.1"
                     /translation="MRIDFASSDRSSLGVEVELEIVDVSSRELRSGASELLPLLGEGH
                     PGGEHPKAKHELLESTVEIITGICGTVAEARQDLLATLAEVRAVAEPRGMALMCSGTH
                     PFSDWAKQDVSPSPRYAQLVEDMQWLARRLQIFGIHVHVGVRSAEKAIAIANALTTYI
                     "
BASE COUNT          518 a         1483 c         1435 g          497 t
ORIGIN      
        1 ctcgtctcga cccggcggcg cccctcacgg cgccggtggc cagactagac tgtcgcctcc
       61 agaagcacac gcgctcggcg ggctggaggg tcgatgttga gggtttcgct tgggaagtcg
      121 gacgcggcgg gcgccaaggc cgacctgctc gccgtcggtg tcttcgagaa tcgggtcctc
      181 gggccgggcg ccgaggaggc cgtggccgcg ctggagacgc cgctcgagcc gctcctggac
      241 gcgagggggt tcaccggcaa ggcgggggag gtcgtcgtgc tccccaccct cggccgcctg
      301 cccgccgcca cgctcctgct cgtcggcctc ggcgagcggg acaaggtcga cgccgacgtg
      361 ctccggaggg ccgccgccgc caccgtccgc cacgccggcg gcgccaagaa ggcggtgacc
      421 acgctcgccc aggccctgcc cggcgacccg gccctcgccg cccgggccgt cgccgagggc
      481 gccgtcctcg ccgactaccg gttcgacaag tacaaggcca aggccaacgg ctccgacccc
      541 cagaggcggc tgtcgagcct cgtcgtggcg cccggcgacg ggaagaaggg cgacctcgcc
      601 gcggccgtcg ccgccgggca ggcgcgggcc gccgccacca acgtcgcccg cgacctgtcc
      661 aacgagcccg ccaacaacct gcacccggcc gacctcgccg acgccgcccg caagctcgcg
      721 ggcaagggcg tcaccgtcaa ggtcaaggac gagaaggcgc tcgccaggga ggggttcggc
      781 ggcatcatgg gcgtcggcca gggcgccgag tccccgcccc ggctcgtcga gatgacctac
      841 cggcccgagg gcgcgcgggc cagcgtggtc ctggtcggca agggcatcac cttcgactcg
      901 ggcggcctgt cgctcaagcc gcccgactcc atgaccgcca tgaagaccga catgtcgggg
      961 gcggcggcga tcgtcggcgt catgagcgtc ctcgacgacc tcggcgtcaa ggtggccgtc
     1021 accggctacc tcgcgtcggc cgagaacatg ccgtcgggca gcgcgacccg gcccggcgac
     1081 gtgctcacca tgaagaacgg caagaccgtc gaggtcctga acaccgacgc ggaggggcgc
     1141 ctcgtgatgg ccgacgccct cgccgtcggc gccgccgccc gtcccgacgc catcgtcgac
     1201 gtcgccaccc tcaccggcgc ctgcgtgatc gccctcggca cgcgctacac cgggctgatg
     1261 agcaacgacg acgccctcgc cgccgagctg ctcgaggcgg ccagggacgc cggcgagccc
     1321 acctggcagc tcccgctgcc cgaggagtac cacaaggagg tcgtcagcga cgtcgccgac
     1381 ctgaagaacg tcggccagcg ctggggcggc gccctcaccg ccggcctgtt cctcaaggag
     1441 ttcgtcgacg accggccctg ggtccacctc gacattgccg gcccctcccg gtcggacagc
     1501 gaggacggct acatccccaa gggctcgacc ggcgccgccg tgcgcaccct gctctcctgg
     1561 ctcgagcggc gcggcagctc gtcgtgaggt tcggcgccac cgggcgcccg gcagcgtcgt
     1621 ggtggcgaag gaagtggacg cctctggtgc cgcggtggat gatcggccgt gccaagcgga
     1681 ccaccaccct cgcgggtggg ccagaaggac tggcgccaac tgagatactt cgcgaccgcc
     1741 gttgagggtc tcggcccgat cctcgcctac gaggttggca cgcttgcggg caccgaggtc
     1801 gagccgcggc ggcagttcga cggccgcaac gacgtggtgt cgttctcggc caagcgtccg
     1861 gacgggttgg cggggttgcg gaccagcgag gacgtcttcg tggaggtctc ggccgccaga
     1921 cgggtgggca ccctccgtgc cctcgtcggg gcactgcgcg acgaccgcgc gctggagcag
     1981 gccctctcgg tctacagcgc gcaggtgcgg cccttgcgcg cccgcatgac gttccgcgtg
     2041 gtggcccgtg tgctgtcgga gcgcgagttc ccgcgcaccg cgctgcgaga cgagctctcg
     2101 cgggtgatcc agcaggcccg gccccgatgg cgggccaccg atccggcggc gctcgagctg
     2161 tgggcgctcg agagcacccc aggccggatc cgcctgggcg tgcggctcag cggcagcgcc
     2221 atgcgccacc gcggcggccg caacctggag cggcccggcg cgctccgccc caccgtggcg
     2281 gccgcgatgg tgctgctggc gggcacgccg acggtgcgcg cgagcgcgac gctgctggat
     2341 ccgtgctgcg ggtcggggac gatcccggcc gaggcggccg cggccggctg gatcccgctc
     2401 ggcgccgacc tcgaccctgg ggcggtcgcg gtggcgcggg ccaaccttgg cggcaccgcg
     2461 cggttgctgc tcgccgacgt ccgccggctg ccgttggccg acgcgagcgt ggccgcgctc
     2521 gccagcaacc tgccgttcgc caggcagtac accgtgcagg ggtcgccggg cgcctggttc
     2581 gcggcggcgc tcgccgagct cgaccgggtg acccaggccc ccgccgccgt cgtgctcctg
     2641 gtgcccacga ccaacgcctt ccagcgcgcg ctcgccgggc gaccgtcact cgccctcgag
     2701 cgccgcctcg acctgcggct gctcgggatg cgcaccgcct tgtggcagct gcggcgccga
     2761 tagccaggac ggaccgtggg ccgcgggcgt tcgcggagca cgccggcgaa cgcctggtgc
     2821 tcgacctggc ccgacggtca cctcgacgtg ggaccctcgg cgcgcacctt cttgacggcg
     2881 aggagggcgt cccgcaggtc gctgatccag tccgcctggt gggtgccgac caggcggacg
     2941 caccaggcca gggcctcgct gcggctgcgg gccacccccg ccccgatgag ggtgtcgagg
     3001 accgcccgct cgcgcagccg cagccgggtc atgaccggga cgctcagggt cgtgaacagc
     3061 gtccgccggt ccccgcagtc ggcgccccac gagaccctgc gggcgaaccg gtgctcggcc
     3121 tcggccgcga cccgcatccg ctgctcgcgg gtggcctccc tgaactcctt ggcccgctcg
     3181 aggcaggcgg ccgcgacggc ggcctccgag gcgcccggcg ggagcgcggg cggggtcagg
     3241 cggcccacca cgaggatctc ctcgccgtcg accgccacct ccgcgggacc ggtgaaccag
     3301 cctgggggga ggcgggcggc gagccagtcg gcgacctcgc cggcgatcgg gtcggtgtcc
     3361 atgcgattac agtattacga aggccgccag aatcgaagcg gctaccctgt cccggtatga
     3421 tcggcgcacg acaaggacag ggagcgctat ctggtgcgga tcgacttcgc ctcctccgac
     3481 cgttcgagcc tgggcgtcga ggtcgagctg gagatcgtcg acgtgagctc ccgggagctt
     3541 cgcagcgggg cgtcggagct cctccccctg ctcggcgagg gccacccggg cggcgagcac
     3601 cccaaggcaa agcacgagct gctcgagtcc accgtcgaga tcatcaccgg catctgcggc
     3661 acggtcgccg aggcccgcca ggacctgctc gcgacgctcg cggaggtgcg cgccgtcgcc
     3721 gagccgcgcg ggatggcgct catgtgctcg gggacgcacc cgttctcgga ctgggcgaag
     3781 caggacgtca gcccgagccc ccggtacgcg cagctcgtcg aggacatgca gtggctggcc
     3841 aggcgtctgc agatcttcgg gatccacgtg cacgtcggcg tgcgctcggc cgagaaggcc
     3901 atcgccatcg ccaacgccct taccacctac atc
//
LOCUS       DASPYY010000646         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2214402, whole genome shotgun sequence.
ACCESSION   DASPYY010000646 DASPYY010000000
VERSION     DASPYY010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214402"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1964
                     /locus_tag="VEP73_10190"
     CDS             <1..>1964
                     /locus_tag="VEP73_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26838.1"
                     /translation="LTVPECAAAGDTLTVAAEATDTAGNTATASRGVRVVSDGVVTGQ
                     ALSDATGLPLADVTVQALSGTGTAFTTDERGRYSMPTGDLALVLRMEKAGLTSVEREV
                     AIESGVGTVPVDARLTPLAEPKTVGAGESLTSGALTLSGPPGTYRLTPLSAQGLPGLL
                     PMGWSPLAAFDLGEAPAASFTATATGLPAASLHLVTYSPTSHAWSLLASLGLPTDGTV
                     SFDVPALGAYALVTPDEADMAIPAPGEILAGVDAQTLPATAASQGTVTPPTMPPTGGT
                     AKGSLTVQSPTPLPSGTVVQAEVTETFKLASGEVASEETRREDIVLYRHPAPEGAALA
                     AELPITPSRTFQTVELTEGEVTLDILSGREGVRGETGGLEALQLDAAEARLVVPAHAL
                     ADDTAVALESHELSSFLPAAPGLTALSEVVVDFATQTLGLSAELSVAAPGVAPDATFL
                     IAKVERDFTLGIPRLVVVALGELVGDRVVNRPTPGLAGITQGGRYVVYRSAGPVGFVG
                     GLVTTSAGPARAVVSAVGDPAQVLPFVALSDFDGRYLLPAIPGPRTTFAALIPGTSFQ
                     ASAPAAVVTGETTPLNLALAGTVTTATVTPADGSLAVPTSVQVQIESAVPLKASSITS
                     GSVRLVRSSDHSPVLLRTVLSASGKLLTVI"
BASE COUNT          230 a          723 c          738 g          273 t
ORIGIN      
        1 cctgaccgtt cccgagtgcg cggcggccgg ggacacgctc acggtggcgg ccgaggccac
       61 cgacaccgcg ggcaacaccg ccaccgccag ccggggcgtg cgcgtcgtct cggacggcgt
      121 cgtcaccggc caggcgcttt cggacgcgac cggcctgccg ctggcggacg tcaccgtgca
      181 ggcgctctcc ggcacgggca ccgccttcac caccgacgag cgcggacgct actcgatgcc
      241 caccggggac ctggccctgg ttctccggat ggagaaggcc gggctcacct ccgtcgagcg
      301 cgaggtcgcg atcgagtcgg gtgtgggcac ggtcccggtg gacgcgaggc tcacgccgct
      361 ggccgagccg aagacggtgg gggcggggga gtcgctcacc tcgggcgccc tcacgctctc
      421 cggcccgccg gggacgtatc ggctcacgcc cttgtccgcc cagggtcttc cggggctgct
      481 gccgatgggc tggtcgcccc tggccgcctt cgacctcggc gaggctcccg ccgcgtcctt
      541 cacggcgacg gccaccgggt tgccggccgc ctcgctgcac ctggtcacgt acagcccgac
      601 gtcccacgcc tggtcgcttc tcgcctcgct gggcttgccg accgacggga ccgtttcctt
      661 cgatgtgccg gcgctcggcg cttacgccct cgtcacgccg gacgaggcgg acatggcgat
      721 ccccgctccc ggcgagatcc tcgccggcgt ggacgcgcag accctgccgg cgacggcggc
      781 cagccagggg acggtgacgc cgcccacgat gccgccgacg ggagggacgg ccaagggatc
      841 gctcaccgtc cagtcgccca cgccgttgcc atcgggcacc gtcgtccagg ccgaggtcac
      901 cgagacgttc aagctcgctt cgggcgaggt cgcctcggag gagacgcggc gggaggacat
      961 cgtcctctac cggcacccgg cgccggaggg ggcggcgctg gcggccgagc tgcccatcac
     1021 gccgtcgcgg accttccaga cggtcgagct gaccgaaggc gaggtcacgc tcgacatcct
     1081 ctccggccgc gaaggggtgc ggggcgagac gggcgggttg gaagcgctgc agctcgacgc
     1141 ggcggaggcg cggctggtgg tgccggccca cgcgctggcc gacgacacgg cggtggcgct
     1201 cgagagccac gagctgtcct cgttcctgcc cgcggcgccc gggctcaccg cgctgtccga
     1261 agtggtggtg gacttcgcga cgcagacgct gggtctgagc gccgagctgt cggtggcggc
     1321 gccgggggtg gcgccggacg cgacgttcct gatcgcgaag gtggagcgcg acttcacgct
     1381 gggcatcccc cggctggtgg tggtggcgct gggcgagctg gtgggcgacc gcgtggtcaa
     1441 ccggccgact cccggcctgg ccggtatcac gcagggcgga cgctacgtcg tgtaccgctc
     1501 ggcggggccg gtgggcttcg tgggcgggct cgtgacgacg agcgcgggtc cggcccgcgc
     1561 ggtggtgagc gcggtcggtg atccggctca ggtcctgccg ttcgtggccc tgtcggattt
     1621 cgacggccgc tacctgctgc cggcgatccc ggggccgcgc acgaccttcg ccgccctcat
     1681 ccccggcacc tcgttccagg ccagcgcccc ggcggcggtg gtgacgggag agacgacgcc
     1741 gctgaacctc gccctggcgg gcacggtcac caccgccacc gtgaccccgg ccgacggctc
     1801 gctggccgtg cccacgagcg tgcaggtcca gatcgagtcg gcggtgcccc tgaaggcttc
     1861 gagcatcacg tccgggagcg tgcggctcgt ccgctccagc gaccactcgc ccgtcctgct
     1921 gcggacggtg ctctcggcga gcgggaagct gctgacggtc atcc
//
LOCUS       DASPYY010000647         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2776582, whole genome shotgun sequence.
ACCESSION   DASPYY010000647 DASPYY010000000
VERSION     DASPYY010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776582"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..3300)
                     /gene="cobN"
                     /locus_tag="VEP73_10195"
                     /pseudo
     CDS             complement(40..3300)
                     /gene="cobN"
                     /locus_tag="VEP73_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
     gene            complement(3297..>3937)
                     /locus_tag="VEP73_10200"
     CDS             complement(3297..>3937)
                     /locus_tag="VEP73_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HYT26839.1"
                     /translation="ATVEDLAWLRSRGLDRALAERARRGGPTFGVCGGYQLLGRRILD
                     PVESGAGEVEGLGLLPVETRFEAGKVLATPSGTAPAFGGAAAGGYEIHHGRTRRLGGE
                     PLLATGGGEDGCRLGTVVGTSWHGLLERDGFRRAFLAWVAGQRGLDWVPGERPFAAVR
                     EERLDALGDLVADHLDAAALDRLIERGAPAGLPVVPPAGPAAALAAGRGATA"
BASE COUNT          471 a         1513 c         1532 g          421 t
ORIGIN      
        1 caccccgtcc agcccgcggc cgtacgcgta gccgcctcag acctcgtaca cctcggacag
       61 gtcggcgtcg gtgcgccagt tccgggcgtc gatcagcggc aggaggccgg cgccgtacgc
      121 gcccggcttg gacccgaaca cccgggtcgt cgcctgccgc tcgccgtgcc cggcggcccg
      181 ctcggcccgg gcgtgcctgg cgacgtagtt ggcgtcgtcg ggctcgtcga ggccggcgac
      241 gagccggacc gcgtcgtcga cgagggtgac gaggttcggg aaggcgtccc ggaagaaccc
      301 gctgatgcgc acggtgacgt cgatgcgggg gcggccgagc tcgcccgggg gcaccgcctc
      361 gagcccggtg acccggcgcg actcctcgtt ccagaccggc cggacgccga gcagggccag
      421 gatctcgccg acgtcgtcgc cctgggtgcg catggccgag gtgccccaga cgacgatccc
      481 gaccgtctcc gggtaggcgc cctcgtcagc caggtacctg gcgagcaggt cgtcggccag
      541 ccgctgcccg gtctcgaacg cgagcgccga cggcagcgcc ttggggtcga cggagtagaa
      601 gttccgcccg gtgggcagca cgttcacgag gccgcgggtc ggcgacccgg agggcccgga
      661 cggcacgaac cgcccgtcga gcccgcgcag caggttgccg agctcgtcgg cggtccgcgc
      721 gaggcgcggg aggacctcgc gggcggcgaa ctcgagcacg gcgacgaccc cggtgtcggc
      781 gctaccgagc acgtcggccg tcaccgcgcg gacgctgccc gcctcccagc ccttcgcgtc
      841 catcgccgcg agcagcgcgc tggccgcctc ctcgagccgg tcgaccaggt ccgcgccggt
      901 caccgccggc cccgggaacc gctcgagcag cgccgccggg ccagcgaccc gccgccccgg
      961 ctccgggctg gtggcggtgg cctccggtga gtcgagggcg acccgccgcc ccggcaccgc
     1021 cagcagcgct ggctcgtcga ggccgtgggc ggcggcgacc gcccggcgca ggcccggcac
     1081 gtcgccggcg ccgaggcgca ggatggcggc gaccaggccg aggcgctgtt ccccgagcgg
     1141 ctgctcgccg aggacgtgga ggccgtcgcg gatgcgggcg tccttgacct cgcacaggta
     1201 gccgtcgagg tggagcacga actcgtcgaa cgcctccggc ggcgcgtcga cgccgaggtc
     1261 gcggtgcagc tcggcggcgc gcaccagctc ccagacccgc gcccggatct cggggagctt
     1321 gaccgggtcg agcgcctgga cctgcgcgta ctcgtccagg agctgctcga gccgggccag
     1381 gtcgtcgtag gtctccgccc gcatcatcgg cgggaccagg tggtcgacga tcacggcgtg
     1441 cgcgcgccgc ttggcctggg tgccttcgcc cgggtcgttg acgatgaacg ggtacagcag
     1501 cggcaggtcg ccgaggacgg cgtcgggggc gcatccggcc gagaggccga ggctcttgcc
     1561 gggcagccac tcgagcgtgc cgtgcttgcc gaggtggacg accgcgtccg cgccgaacac
     1621 ccggtccagc caccagtacc cggccaggta gtggtgggac gggggcaggt cggggtcgtg
     1681 gtagatcgcg accgggttct cgccgaagcc ccggggcggc tgcacggcca ggaagacgtt
     1741 gccgaggcgc aggccggcga ggaccgggtc gccgccgtcg acgtacagct cgccgggcgg
     1801 ctccccccag tgccgcacga ccgcctcccg cagcgggacc ggcaggccag cgaaccatcc
     1861 ggcgtaggcg cggccgtcga cgcgcccgga ggcccgcgcg agctgctcct cggtgaggaa
     1921 ctcggcgtcc tgcccgcccg ccgcgatcac ctcgtgcacc aggcggtcgg ggtcccagtc
     1981 ccccgggtgg ccggcctcgg gccgcgcttc ggggccctcg acccggtagc cggcctcgcg
     2041 catggcggcc agcagccgca ccgcgctcgc gggcgtgtcg agcccgaccg cgttgccgac
     2101 cctggcgtgc ctggtcgggt agttcgacag cacgatcgcg acccgcttgt cggcgttggg
     2161 gacgtgccgc aggcgggcca gccgcgtcgc gatcccggcg agccgggccg cccgctcggg
     2221 gtcggcccgg tagaccacca cctgggcgcc ggctccaggg ccttggccgg gcccgtcggt
     2281 gccgggcgcc gcggaggccg gcagctcgac cggctccttg aacgagaacg ggacgccgac
     2341 gatccggccg tcgaactccg ggatggcgac ctgcatggcc gcgtccagtg ggctgaggcc
     2401 cccgtcgctc gccgcccagg cggcgcggcc gacggtcgcg cagatgccct ggacgaccgg
     2461 cacgtcgagc gcctcgaggg cgggcgcctc ccacgcgagc cacgcctccg gccggtcccg
     2521 gccagcaccg gccagggcgt cggccgcgcc cgagccgccc gacgcgagca ccgtgacgac
     2581 cagcgcgtcc accccgtggc cggcgagcag gtcgcaggct gggacccgcc cgcgggcgtc
     2641 ggggcgcagc gagtagcaga acaccggaag cgggttcgcc ccggccgcct ccagcgcgtc
     2701 gaccagggtg tcgacgaagg cggtgttccc gctcatccag tgggtgcggt agaagacgat
     2761 cccgacggtg ggccgggccg ggtcgcggcg ctcgagcagc tccggccggt agaccccgtg
     2821 gtcgggcagg gccgccggcg gctcgaagcc gaacccggtg tagagcagtg tgtcggcgag
     2881 gaagcggaac aggttggcgg tgttggccac gccgccgtgg cggaggtact cgaacgcctc
     2941 ggcgaccacc ccggccggcg cggtcgacgc ggcggcgagc tcggcgtccg gctcggcctc
     3001 gcccccgagc gcgagcagcg gcacgccgcc cctggcgcac cgccaccgga gcaggtcgaa
     3061 ccctgccggc caggcccggc ggccgccgag cagccggatc accacggcgt ccggggtccg
     3121 gggggtgggg ggatccgggg tcccagggtc ggccgggaag tgccggtcga ggaacgcggc
     3181 cggctccgcg atctgggacg ggttggcggc cagcaccggc ccgagcccgg cgggcagctc
     3241 ggccgcggcc ctcgccgcgg ccaggacctc cgtgtcggcg gtggtgagga agaggatcac
     3301 gccgtcgccc ccctcccggc cgcgagcgcg gccgccgggc ctgccggggg gaccaccggc
     3361 aggccggccg gcgcgccccg ctcgatcagg cggtcgaggg ccgcggcgtc gaggtggtcg
     3421 gcgacgaggt ccccgagggc gtcgaggcgc tcctcgcgca cggccgcgaa gggccgctcc
     3481 cccggcaccc agtcgagccc ccgctggccg gccacccagg ccaggaaggc gcggcggaag
     3541 ccgtcgcgct cgagcagccc gtgccaggag gtgcccacga ccgtcccgag ccggcacccg
     3601 tcctcgccgc cgccggtggc gagcagcggc tcgccaccga gccggcgggt ccgcccgtgg
     3661 tggatctcgt agccgccggc ggcggcgccg ccgaaggccg gcgccgtccc gctcggggtg
     3721 gccagcacct tcccggcctc gaaccgggtc tcgaccggca gcaggccgag cccctcgacc
     3781 tcgccggcgc cgctctcgac cgggtcgagg atgcgcctgc cgaggagctg gtagcccccg
     3841 cacaccccga aggtcggccc cccgcgcctg gcccgctcgg cgagcgcacg gtccaggccg
     3901 cgggagcgca gccacgccag gtcctcgacg gtcgcct
//
LOCUS       DASPYY010000648         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2776605, whole genome shotgun sequence.
ACCESSION   DASPYY010000648 DASPYY010000000
VERSION     DASPYY010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776605"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1851)
                     /locus_tag="VEP73_10205"
     CDS             complement(<1..>1851)
                     /locus_tag="VEP73_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HYT26840.1"
                     /translation="ADEAARRLLDEALEAAAPVTIMGVGYARFAGYPDAAPATWLLER
                     GLLLPVSYNQFVVPREAALGLRGGRVFPDWPEPPAPPHLDPLPDPAGRAAAAAMRAVT
                     AAEGIVTRLDRQPLALIQSGTIAVRDLRRLATELDLAEDEAGLLVDLLTEADVIMVGG
                     PWEQRSLGLRPEADAWLASTRARRWADLAVAWRDRDLALEDHLLPRYGLAPSGADKVR
                     PLNGRRESGAVTRRRGLLEVLARAPDGHGMAFEALGGRLRWRMPTLWSGRSVDLARVV
                     LDAAALLGLVAVIGGRAGPGPATVAWCADAGPDELAAALGELLPDGSGRFLVAGDLTV
                     VAPGGLAPDVESRLAMLADRESGGGAATWRIGEASLRRAFDEGLSGEGVLAFLRERSD
                     TPLPQALEYLVEDAARRHGRLRVGGASTYLRGEPALVTQTVRSAAGRRLGLRELGPGV
                     AVTHRSQRELLTALRKAGEAPLAEEPDGTARLEARKTVRHAPRAAQLDYRRAGQPAEP
                     AAAPAEALARLRASTGAGGGPGQAVEEREAGLVTAPAEIAALCQRAAGVQGAVEIAYR
                     ARDGEETVRTVEPYGVRAGLLTAWCRLRQAERTFDLGQILWARPVAEPRRP"
BASE COUNT          194 a          704 c          779 g          174 t
ORIGIN      
        1 ggccgccggg gctcggcgac cgggcgggcc cacaggatct ggccgaggtc gaacgtccgc
       61 tcggcctgcc tcagccggca ccaggcggtg aggaggccgg cgcggacgcc gtacggctcg
      121 accgtgcgca cggtctcctc cccgtcgcgg gcccggtagg cgatctcgac ggcgccttgg
      181 acgcccgcgg cgcgctggca gagggcggcg atctcggccg gcgcggtgac gaggccggcc
      241 tcgcgctcct ccacggcctg gcccgggccg ccgccggcgc cggtcgacgc gcgcagccgg
      301 gccagcgcct cggccggggc ggcggccggc tcggccggct ggccggcgcg gcggtagtcg
      361 agctgcgcgg cccgcggcgc gtggcggacg gtcttgcggg cctccaggcg cgcggtgccg
      421 tcgggctcct cggccagcgg cgcctccccc gccttgcgca gggcggtgag cagctcgcgc
      481 tgggagcggt gggtgaccgc cacgccgggg ccgagctccc gcaggccgag ccggcggccg
      541 gcggccgagc ggaccgtctg ggtgacgagc gcgggctcgc cgcgcaggta cgtgctggcc
      601 ccgccgaccc gcaggcggcc gtgccggcgg gcggcgtcct cgaccaggta ctcgagggcc
      661 tgggggagcg gggtgtcgga gcgttcgcgc aggaacgcca gcacgccctc cccggacagc
      721 ccctcgtcga aggcgcgacg caggctcgcc tccccgatcc gccaggtggc cgcgccgccg
      781 cccgactcgc ggtcggccag catcgcgagg cggctctcca cgtccggggc caggccgccg
      841 ggcgccacca cggtcaggtc gccggccacg aggaaccggc ccgagccgtc cggcagcagc
      901 tccccgaggg cggcggccag ctcgtcgggg ccggcgtccg cgcaccaggc gacggtcgcc
      961 ggtcccgggc cggcccgccc gccgatgacc gcgaccaggc cgaggagggc ggccgcgtcg
     1021 aggacgaccc gggcgaggtc gacgctccgg ccgctccaga gcgtgggcat gcgccagcgc
     1081 agccgcccgc cgagcgcctc gaaggccatg ccgtggccgt cgggcgcccg cgcgaggacc
     1141 tcgagcaggc cgcggcgtcg cgtgacggcg cccgactcgc ggcggccgtt gagggggcgg
     1201 accttgtcgg cgccggaggg ggcgaggccg tagcggggca gcaggtggtc ctcgagggcg
     1261 aggtcccggt cgcgccaggc cacggccagg tcggcccagc gccgggcgcg cgtcgaggcg
     1321 agccacgcgt cggcctcggg ccgcagcccg agcgagcgct gctcccaggg gccgccgacc
     1381 atgatgacgt cggcctcggt gagcaggtcg acgagcagcc cggcctcgtc ctcggccagg
     1441 tccagctcgg tggcgaggcg gcgcaggtcg cggacggcga tcgtgcccga ctggatgagg
     1501 gcgagcggct gccggtcgag gcgcgtcacg atgccctcgg cggccgtgac cgcccgcatg
     1561 gccgcggccg cggcccggcc cgccgggtcc ggcagcgggt cgaggtgggg cggggcgggc
     1621 ggctcgggcc agtcggggaa cacgcggccg ccgcgcaggc cgagggctgc ctcgcgcggg
     1681 accacgaact ggttgtacga cacgggcagg agcagcccgc gctcgagcag ccaggtggcg
     1741 ggggcggcgt ccgggtagcc ggcgaagcgg gcgtagccga cgcccatgat ggtgacgggg
     1801 gcagccgcct ccagcgcctc gtcgagcagg cgcctggccg cctcgtcggc c
//
LOCUS       DASPYY010000649         7523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_248419, whole genome shotgun sequence.
ACCESSION   DASPYY010000649 DASPYY010000000
VERSION     DASPYY010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248419"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VEP73_10210"
     CDS             complement(<1..541)
                     /locus_tag="VEP73_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26841.1"
                     /translation="MRPLDTSATAASRQHGAGPALPGAGTCLVVDAGRTGCRAVLYAG
                     DGDAGDGVVAGPGLPGVADGEGSAEPFVAVLAPLLRQLSLTSRPPSSRSSPGRPLDAV
                     CVGMTGVLRPGPAAAAVADAVHALLPVRRVLVTSDVVTGYCGAVGLGPGVVAAAGTGV
                     IAIALGERGVARVDGWGYIL"
     gene            711..1517
                     /locus_tag="VEP73_10215"
     CDS             711..1517
                     /locus_tag="VEP73_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYT26842.1"
                     /translation="MLPAGLSSQRPKGEQLEEILEGFVAALAPGMALPSERLLAERYG
                     LARATVSQVIEGLAVKGLLYRVHGSGTFVAEPKFRQPETLTSFSEDMRARGMTPGSVV
                     LGREVVAASEIIARQLEVEPGAPVLHVERVRTADGEPMALERTYLPAERFPGLGEVDL
                     TDVSLYEVLERRWGVLVAVADQWFSVMRLTEGEAKLLGVGGGQPALLFQRVTRDPAGR
                     VVEYVRSLYRGDRYEVHSRLRRPGLSPEAPGADRQPADTPVTRPVTRPSG"
     gene            complement(1498..2526)
                     /locus_tag="VEP73_10220"
     CDS             complement(1498..2526)
                     /locus_tag="VEP73_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYT26843.1"
                     /translation="MAAEIAEQPEAVARTLDALRPLRGGLAGLAAGARHVLFLARGSS
                     DNAATYGRYLCEVQAGRAASLGAPSLATHYRARTDLSGVLAVALSQSGETGEIVDALR
                     WAAERGARTVAVTNAEGSTLARTAELALVTRAGTERAVPATKTYTTQLAALAVLAAAL
                     GPDDPAFDAALARAPEAIAGLLDTREAAAALAGELAGIRGMVVSGRGFAYSTALELAL
                     KLKETCYLTAIGLSHADLEHGPIAVVGPGTPALLVAAGDGPVLPGMTALARSVAERGG
                     RAYGIGGDAAFRGACAATLPGPALPEPLAPLALVVPGQLLVEALARRLGFDPDRPRGL
                     TKVTQTGG"
     gene            complement(2538..3719)
                     /gene="nagA"
                     /locus_tag="VEP73_10225"
     CDS             complement(2538..3719)
                     /gene="nagA"
                     /locus_tag="VEP73_10225"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HYT26844.1"
                     /translation="MQVLSAPRVVAGHRVLSPGAVVVEGDRIAAVLPGRPAAHRDHLR
                     LEHGILAPGLVDLQVNGYGGVDLARAGPEGWARVAEACAATGVTSFLPTFITAPVEAL
                     AARLEQARAARAGQTRATATRVRPGARILGVHMEGPFLSPARAGAHDPGAMLDPAPEL
                     VDRLLEAGEGILALMTLAPERDGALDAVKRLAGAGVVVSVGHSDATAAEVAAAADAGA
                     RKVTHLFNAQRGLGHREPGVAGQGLADPRLTLGLIADLHHVHPAVCQVVLAAAGGRVA
                     LVTDATAAAGMPPGRYDLGGVPVELPPEGPPRRADGTIAGSALRLDEAVANAAGLGLG
                     LADAVGAATRVPADLLGRADLGRLAPGALADLVWLGDDLRTLATWVGGARAPAPTSEE
                     A"
     gene            complement(3845..4093)
                     /locus_tag="VEP73_10230"
     CDS             complement(3845..4093)
                     /locus_tag="VEP73_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYT26845.1"
                     /translation="MDDYFRREGLVRPRHDRVLGGVCAGLGRRFGLGPWQARILFVLI
                     LMLIPGSQIIIYPILWVLMPSEPRAAELTPGGPAGIAS"
     gene            4345..4782
                     /locus_tag="VEP73_10235"
     CDS             4345..4782
                     /locus_tag="VEP73_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HYT26846.1"
                     /translation="MTGFDRVAWFPLCAGLTALGLVASWLAARRRGGAAGLRGAAWSL
                     LPLAAYLTGLVTLLWQIGTALTRWVAGFVLSPTVWAGVAVTGLAAICFLVSGFLRRRA
                     LPGTPSRGSIEPPGRAAPPARPGARGDGEFADVEEILRRRGIR"
     gene            4991..6025
                     /locus_tag="VEP73_10240"
     CDS             4991..6025
                     /locus_tag="VEP73_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYT26847.1"
                     /translation="MTDYALLILPATNRVYADASVRLTQAELEVFNRSVLDGRLREVS
                     EAQIGGVPYVAFSADRLGGRDLAFLSNLSSIYALFEVEGGLLRPLELHPLVRFDSDLI
                     TIPKYQGKTNEYFTKLLLNVTVLSTRFPHEMLERSFRVLDPLCGRGTTLNQALMYGYD
                     AAGMDTSGKDFDAYAAFIRTWLQRKRVKHQAEVESVRRNRRVVARRLHVTLGATRELY
                     KAGSTQELTVVNADTGRGLEFFRPGSFDVLVADAPYGVQHGSRTPGGAASRSPVGLLV
                     SAVPVWAELLRSGGALGIAWNTFVARREEAAGVLAASGLEVLRSGAYLGFQHRVDQAV
                     SRDVLVARKP"
     gene            complement(6194..7081)
                     /locus_tag="VEP73_10245"
     CDS             complement(6194..7081)
                     /locus_tag="VEP73_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26848.1"
                     /translation="MRTQRAGMIAAGVAVVVLALTAAVLLFTNRSKPTRAAATATTAP
                     AADAAATTGAAATAGDATATTAATGTTAAGPTSTTTGADALARVSPAGALLTALREPF
                     NVDGAADLPCTAFLDNGHVLGPSHCGRVHLAPTGRLAIWVTGRTPDTKAWTVRIFAYD
                     GNGSWSERLQAVEEAPGLTWTGVDVVPARLSAGATTLIAMATNQGTGVYLGYDLVRWP
                     RSTGQPTVVAHRGDLAHGFFRVRQTPGGTVLDDYGANYDDGAPSCCPNRYDRSRIAFR
                     AGVFRYLVREQVPASAIGR"
BASE COUNT          905 a         2766 c         2906 g          946 t
ORIGIN      
        1 cgaggatgta gccccagccg tccacgcggg cgacgccccg ctcgccgagg gcgatcgcga
       61 tcacgcccgt gcccgcggcg gcgaccaccc cgggcccgag gccgacggcc ccgcagtagc
      121 cggtcaccac gtcgctcgtg accagcaccc ggcggaccgg cagcagcgcg tggacggcgt
      181 cggcgaccgc ggcggcggcc gggccggggc gcagcacgcc ggtcatgccg acgcagacgg
      241 cgtccagcgg ccggccgggc gacgagcgtg aggaaggcgg gcgcgaggta agcgagagct
      301 ggcgcagcag gggggcgagc acggccacga acggctcggc ggagccctcg ccgtcggcga
      361 cgccgggcag gccgggccct gccacgacgc cgtccccggc gtcgccgtcg cccgcgtaga
      421 gcacggcccg gcaccccgtc ctgccggcgt ccacgaccag gcaggtcccg gcccctggca
      481 gcgcgggacc ggctccatgc tggcgggacg ccgcggtggc ggaggtatca agtggtcgca
      541 tgctcgccgt ctcgtcgtcg tgacatcggg tcagattgag cgcaggggca agcctgattg
      601 gcctagtcca ctttgtcaag gcacgcccac ggtgggccgc tgccggccgc gctcaccggg
      661 cggctacact tgccgtcatg aggacgaggc gccagggaga gctgatcgcg atgctgccgg
      721 cggggctctc ctcgcagcgg cccaagggcg agcagctcga ggagatcctc gagggcttcg
      781 tcgccgcgct cgcgcccggg atggcgctgc cgtccgagcg gctgctggcc gagcgctacg
      841 ggctcgcgcg ggcgactgtc agccaggtca tcgagggcct cgccgtcaag gggctcctct
      901 accgcgtcca tggcagcggg accttcgtcg ccgagcccaa gttccggcag ccggagaccc
      961 tcacgtcctt ctcggaggac atgcgcgcgc ggggcatgac gccaggctcg gtcgtgctcg
     1021 gccgcgaggt cgtagcggcc agcgagatca tcgcccgcca gctcgaggtc gagcccggcg
     1081 cgcccgtcct gcacgtcgag cgcgtgcgca ccgccgacgg cgagccgatg gcactcgagc
     1141 gcacctacct gccggccgag cgcttcccgg ggctcggcga ggtcgacctc accgacgtct
     1201 cgctctacga ggtgctcgag cggcgctggg gggtgctggt cgcggtggcc gaccagtggt
     1261 tcagcgtcat gcgcctgacc gagggcgagg cgaagctgct cggggtcggc ggcggccagc
     1321 ccgccctcct gttccagcgg gtgacccggg accccgccgg ccgagtggtc gagtacgtgc
     1381 ggtcgctcta ccggggcgac cgctacgagg tccacagccg cctccggcgt cccggcctca
     1441 gccccgaggc gccgggcgcc gaccgccagc cggcggacac gcccgtcacc cgccccgtca
     1501 cccgcccgtc tgggtgacct tggtgaggcc gcggggccgg tcgggatcga agcccagccg
     1561 gcgcgccagc gcctcgacca ggagctggcc aggcacgacg agggccaggg gcgcgagcgg
     1621 ctcgggcagg gcgggcccgg gcagggtcgc ggcgcacgcc ccgcggaagg ccgcgtcccc
     1681 gccgatcccg taggcccggc cgccccgctc ggccaccgag cgggccagcg cggtcatgcc
     1741 gggcagcacc ggcccgtcgc ccgccgcgac cagcagggcc ggggtgcccg ggccgaccac
     1801 ggcgatcggg ccgtgctcca ggtcggcgtg cgacagcccg atcgccgtca ggtagcaggt
     1861 ctccttcagc ttcagcgcga gctcgagcgc ggtggagtag gcgaagccgc ggccggacac
     1921 gaccatgccc cgtatcccgg cgagctcgcc cgcgagcgcc gcggcggcct cacgggtgtc
     1981 gagcaggccg gcgatcgcct cgggagcccg tgccagggcc gcgtcgaagg cggggtcgtc
     2041 cgggccgagc gcggcggcga gcacggccag cgccgcgagc tgggtggtgt aggtcttggt
     2101 cgcgggcacc gcgcgctcgg tcccggcccg ggtgacgagc gccagctcgg ccgtgcgggc
     2161 gagcgtcgag ccctccgcgt tggtcaccgc gacggtccgg gcgccccgct cggccgccca
     2221 gcgcagcgcg tcgacgatct ctccggtctc gcccgactgg gagagggcga cggcgagcac
     2281 tcccgagagg tcggtgcgcg cccggtagtg ggtggccagc gagggcgcgc cgaggctggc
     2341 ggcgcgcccg gcctggacct cgcacaggta gcggccgtag gtggcggcgt tgtccgacga
     2401 gcccctcgcg aggaacagga cgtgcctggc gccggcggcc aggccggcca gcccaccccg
     2461 gagaggccgg agggcgtcca gcgtgcgggc caccgcctcg ggctgctcgg cgatctcggc
     2521 cgccatcgcc gtcatcttca cgcctcctcg ctggtcggcg cgggcgcccg cgccccgccg
     2581 acccaggtgg ccagggtgcg gaggtcgtcg ccgagccaga ccaggtcggc aagggccccg
     2641 ggggcgagcc ggccgaggtc ggcccgcccg agcaggtcgg cggggacccg tgtggccgcg
     2701 ccgaccgcgt cggccaggcc gagcccgagc ccggcggcgt tggccaccgc ctcgtccagg
     2761 cgcagcgccg agccggcgat ggtgccgtcg gcccgccgcg ggggtccctc cggaggcagc
     2821 tcgaccggca cgccaccgag gtcgtagcgg ccgggcggca tgcccgcggc ggccgtggcg
     2881 tcggtgacga gggcgacccg cccgcccgcg gcggccagca cgacctggca gacggccggg
     2941 tggacgtggt ggaggtcggc gatcagcccg agggtgaggc gcgggtcggc cagcccctgc
     3001 ccggcgacgc ccggctcccg gtggccgagc ccgcgctggg cgttgaacag gtgcgtcacc
     3061 ttccgcgcgc cggcgtcggc ggccgcggcc acctcggccg cggtcgcgtc gctgtgcccg
     3121 accgagacca cgaccccggc cccggccagc cgcttgaccg catcgagggc cccgtcgcgc
     3181 tcgggcgcca gagtcatcag ggccaggatg ccctcgccgg cctcgagcag ccggtcgacc
     3241 agctcgggag ccgggtcgag catcgcgccc gggtcgtggg ccccggcccg ggcgggggag
     3301 aggaacggac cttccatgtg gacgccgagg atccgggctc ccggccgcac cctcgtcgca
     3361 gtggcgcgcg tctggcccgc gcgggccgcg cgggcctgct ccaggcgggc cgcgagcgcc
     3421 tcgaccggcg cggtgatgaa ggtgggcagg aacgaggtga cccccgtggc cgcgcacgcc
     3481 tcggcgaccc tcgcccaccc ttccgggccg gcccgggcga ggtccacccc gccgtagccg
     3541 ttcacctgca ggtcgaccag gcccggggcg aggatgccgt gctcgaggcg gaggtggtcg
     3601 cggtgcgcgg cgggccggcc cgggagcacg gccgcgatgc ggtcgccctc caccaccacc
     3661 gcgccggggc tgagcacgcg atggccggcg acgacgcggg gcgcggagag gacctgcacc
     3721 ggggcgcctc cgttccgggg cggtcgggga cggcggcctg ccgccgaccg ggcggtgggc
     3781 cgccgtccag tctggcctgg accggcgacc gcgtccagca gccccggcgc cggggcctcc
     3841 ccggtcagga ggcgatgccg gccggaccgc cgggggtgag ctcggccgcc cggggctccg
     3901 agggcatcag cacccacagg atcgggtaga tgatgatctg gctgccgggg atcagcatga
     3961 ggatgagcac gaagaggatc cgtgcctgcc aggggccgag gccgaagcgc cgcccgaggc
     4021 cggcgcagac gccgccgagc acgcggtcgt gacggggccg gaccaggccc tcccggcgga
     4081 agtagtcgtc catgttcgtt gcgtccttct cgtggtgcca tgtggcgagt tgccaccgac
     4141 atcgtgcccc gggccgggcg ccgccgccat cggggatccc cctggaagat cccggacccg
     4201 gccggcgcgc gctcagggtc cctcacctcg gcccggtcaa gagcacctgt cgctcccagt
     4261 tcgcgcacgt cgtcggccag gcagcgcccg gccctccggc gtggcacgat agcaggggtg
     4321 tccgcgcgac ggaggttcgg tgacatgacg ggattcgacc gggtggcgtg gttccccctg
     4381 tgcgccgggc tgaccgcgct cggcctggtc gcgagctggc tcgcggcacg gcggcggggc
     4441 ggcgccgctg gcctgcgcgg cgccgcgtgg tcgctcctgc cgctcgcggc ctacctgacc
     4501 ggtctcgtca cgctgctgtg gcagatcggg accgcgttga cccgctgggt ggcggggttc
     4561 gtcctctcgc cgacggtgtg ggcgggggtc gccgtcaccg ggctcgccgc catctgcttc
     4621 ctcgtctccg gcttcctgcg gcgccgcgcg ctgccgggca cgccgtcaag ggggtcgatc
     4681 gagccgcctg ggcgcgcggc gccgccggcc cgcccgggcg cgcgcggcga cggcgagttc
     4741 gccgacgtcg aggagatcct ccgccgccgc ggcatccgct gaggcacccg gccgctgcca
     4801 gggcggccgt ggcgcggccg actgggcgcc tggtgttcgg cgccacaccc gttcgggctc
     4861 ctgcttcggc agcgccgtcg tcgactgcga cgccgacgcc aagtgacgcc gcctgccgtg
     4921 cccgggccta ctatctcggc gtgacctctc ctgacgccgg cttcccgccg ccgcgcccgg
     4981 gcgggcgcga gtgaccgact acgccctgct gatcctcccg gcgacgaacc gggtctacgc
     5041 cgacgcctcg gtacgcctga cccaggccga gctggaggtg ttcaaccggt cggtcctcga
     5101 cggtcgcctg cgcgaggtgt cggaggccca gatcggcggg gtgccgtacg tcgccttctc
     5161 ggcggaccgg ctgggcggcc gggacctggc cttcctgtcg aacctctcct cgatctacgc
     5221 gttgttcgag gtcgaaggcg ggctgctgcg accgctggag ctccacccgc tggtgcggtt
     5281 cgacagcgac ctgatcacca tcccgaagta ccaggggaag accaacgagt acttcaccaa
     5341 gctcctcctg aacgtgacgg tcctgtcgac caggttcccg cacgagatgc tcgagcggag
     5401 cttccgcgtc ctggacccgc tctgcggccg gggcacgacg ctgaaccagg cgctgatgta
     5461 cggctacgac gccgccggga tggacaccag cgggaaggac ttcgacgcct acgccgcctt
     5521 catccggacc tggctccagc gcaagcgggt caagcaccag gccgaggtgg aatccgtccg
     5581 ccggaaccgc cgcgtcgtgg cccgccgcct ccacgtcacc ctcggggcga cccgggagct
     5641 gtacaaggcg gggagcacgc aggagctcac ggtggtcaac gcggacaccg gcaggggact
     5701 cgagttcttc cggccgggct cgttcgacgt cctcgtcgcc gacgcgccgt acggcgtcca
     5761 gcatggcagc cgcaccccgg gcggcgccgc gtcacggagc cctgtcggcc tgctcgtgtc
     5821 ggcggtgccg gtctgggcgg agctgctgcg gtcgggaggc gcactcggca tcgcatggaa
     5881 cacgttcgtg gcgcggcggg aggaggcggc cggtgtcctg gccgccagcg gcctcgaggt
     5941 gctgcggtca ggcgcctatc tggggttcca gcaccgcgtg gaccaggcgg tcagcaggga
     6001 cgtcctcgtc gcccgcaagc cctgagggcc gggtcgcgaa gtcgcgtcgt cggcatgggg
     6061 acgaagccgc tcgtttcccg gagccttccg cttcgaggtc ccggtcgatc atgccgcacc
     6121 gcggcggcga gtcccaggag atccccaggg ggactgacca gtacgtccgt taccggaacc
     6181 tgagagagcc gtttcagcgg ccgatggcgc tcgccggaac ctgctcgcgt accaggtagc
     6241 ggaacacgcc ggcccggaac gcgatgcgcg agcggtcata gcggttgggg cagcagctcg
     6301 gggcgccgtc gtcgtagttc gccccgtagt cgtccagcac ggtgccgccc ggagtctggc
     6361 ggacccggaa gaagccgtgg gccaggtcgc cccggtgggc gaccacggtg ggctggcccg
     6421 tgctgcgcgg ccagcggacc aggtcgtagc cgaggtacac gccggtgccc tggttggtcg
     6481 ccatagcaat gagcgtcgtg gccccggcgg acagcctcgc cgggacgacg tccacgccgg
     6541 tccaggtcag gcccggcgcc tcctcgacgg cctggagtcg ctccgaccac gagccgttgc
     6601 cgtcgtaggc gaagatgcgc acggtccacg ccttggtgtc gggggtgcgg ccggtgaccc
     6661 agatggcgag cctgccggtc ggggccagat gcacccggcc gcagtggctg ggccccagca
     6721 cgtggccgtt gtccaggaat gcggtgcagg gcaggtcggc cgcgccgtcg acgttgaagg
     6781 gctcccgcag cgcggtcagg agcgcccctg cgggtgagac cctggccagc gcgtccgcgc
     6841 cggtggtggt cgaggtgggg cccgccgcgg tcgtcccggt ggccgcggtg gtggcggtgg
     6901 cgtcacctgc cgtggcggct gcgccggtcg ttgcggcggc gtcggcggca ggcgcggtcg
     6961 tggcggtggc cgcggcccgc gtcggcttcg agcggttggt gaagagcagg acggcggcgg
     7021 tgagcgcgag gacgaccacg gctactccgg cggcgatcat cccggcccgc tgggtacgca
     7081 tgacgacccc ttctgtctgg catggcggta cgtatgccag cgtcccgcga ccaggtcaac
     7141 ggtcgtgcct tgtgcgattg cgatcggcta acggctccca ccaggcgtga caagcgcgta
     7201 acctgcacgt tcccaccgcc gagatgcctc tggcacggcc tcactccaag caacggtcag
     7261 gcgtccaaac ctcgcccgac gccgaggagt gtcgagcgga cggagtaccg ggaggcggcc
     7321 gacgcgccac tcgcgggtgc ggctgggctt cggtcgcggg ggcctgcggc gctgccatca
     7381 ggggtcgtcg ggtttacacc tcgtcgagcc ggtgcagcac ccgttggttg gcgtcgtcgc
     7441 tgagggcgaa cgggtcgtct ggccagtcgt ctgggtcttg gccgagcagc tcggtcgcct
     7501 ccaggaccca cgtcgtcgca ccc
//
LOCUS       DASPYY010000650         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1372816, whole genome shotgun sequence.
ACCESSION   DASPYY010000650 DASPYY010000000
VERSION     DASPYY010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372816"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /locus_tag="VEP73_10250"
     CDS             <1..2296
                     /locus_tag="VEP73_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HYT26849.1"
                     /translation="AGRDRGAAGRRDRDRIVRAGFERMRAARPAALAADSVAARITLG
                     NFEDDFGRVAHADWVIEAIVEQLEPKQELAARIEKTASAGALVSSNTSGIPLASIAEG
                     RSGEFRRRFLGTHFFNPPRYLKLVELIPTADTDPEVLEAMRAFVEGVLGKGAVVAKDT
                     PNFIANRLGSFAGMHGLRYAFEHGYSIEEVDALTGPLVGRPRTASFRLADQVGIDVMV
                     GVADNLHRLARDDESREELLAPEPLRRLVAAGRLGIKTGGGFYRRDRRDGRTVFDVVD
                     TATLGHGDAGTLGYRPAEEPDLPVVEAAREHRDLGERLRFLLDKAEEGDRGAVYLRDT
                     LLPALAYASRRIPEIADSPAEVDHAMEWGFGHQAGPFRTWDLLGVRGTTERMRRLGLE
                     VAPWVDDFLGAGNESFYRDGAAYQPSSGTYEPVRTDPDAIDLAALKAEGREVLGNDSA
                     SLVDLGDGVLCLELHAPASAVDARVIQLGHQALAELEGGDWKGLVIGGRAANFCVGAN
                     LAEVGMAAYQRAYDQVKEASRSLQELLMGFRFAAKPVVAAPHGQTLGAGCEICLHADR
                     VVAALETYMGQVETAVGLIPAGGGCKELVRRIVSPAMRVQDAPPLPFVQRVFETIALA
                     KVGTSAVESRELGFLADDDRIVLNADHLLTAAKREVLELAPGYRPPPREASVFAAGSP
                     TLAALELGVRTQQWGGYASGYDGVVATHLASVLCGGELSAPQWVTEDYLLELEREAFV
                     ELLRNEKTMERIQAMLTTRKPLRN"
     gene            2320..>2977
                     /locus_tag="VEP73_10255"
     CDS             2320..>2977
                     /locus_tag="VEP73_10255"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYT26850.1"
                     /translation="MKEAVLVSGARTAVGRAPKGALRAARPDDMAAAAITEAIKRADG
                     LDPAEVEDVVLGCAIPEGTQGMNVARVAALRAGLPESVPGQTVNRFCSSGLQTIASAA
                     ERIMAGFATTIVAGGTEHMSSTPVTSANFAPNPEVVRSFPEVYMSMGLTAERVAERWK
                     VSREDQDEFALRSHRRAAEAVDSGRFDPEIVPLPVTLDWVDGDERRHSETTFARDEGP
                     R"
BASE COUNT          409 a         1104 c         1095 g          369 t
ORIGIN      
        1 ggcagggcgc gaccgggggg ccgccgggcg gagggaccgc gaccgcatcg tgcgggccgg
       61 cttcgagcgc atgcgggcgg cccggcccgc cgccctcgca gccgacagcg tggccgccag
      121 gattacactt ggcaatttcg aggacgattt cggccgggtc gcgcacgccg actgggtgat
      181 cgaggccatc gtcgagcagc tcgagcccaa gcaggagctg gccgcccgca tcgagaagac
      241 cgcgagcgcc ggggccctgg tctcgtccaa cacctccggc atcccgctcg cgagcatcgc
      301 cgagggccgc tccggggaat tccggcgccg gttcctcggc acccacttct tcaacccccc
      361 gcgctacctc aagctggtcg agctgatccc gaccgccgac accgaccccg aggtgctcga
      421 ggccatgcgc gcgttcgtcg agggggtgct cggcaagggc gcggtcgtcg ccaaggacac
      481 gccgaacttc atcgccaacc gcctcggcag cttcgccggg atgcacggcc tgcggtacgc
      541 cttcgagcac ggctactcga tcgaggaggt cgacgcgctc accggcccgc tcgtcggccg
      601 gcccaggacg gccagcttcc ggctcgccga ccaggtcggc atcgacgtca tggtgggcgt
      661 ggccgacaac ctccaccggc tcgcccgcga cgacgagagc cgggaggagc tgctcgcgcc
      721 cgagccgctg cggcgcctgg tcgcggccgg ccggctgggc atcaagaccg gcggcggctt
      781 ctaccggcgg gaccggcgcg acggccggac cgtcttcgac gtcgtcgaca ccgccacgct
      841 cgggcacggg gacgccggca cgctcgggta ccggccggcc gaggagcccg acctcccggt
      901 cgtcgaggcg gcccgcgagc accgcgacct cggcgagcgg ctgcggttcc tgctcgacaa
      961 ggccgaggag ggcgaccggg gcgcggtgta cctgcgcgac accctgctgc ccgccctcgc
     1021 ctacgcgtcg aggcggatcc ccgagatcgc cgactcgccc gccgaggtcg accacgcgat
     1081 ggagtggggc ttcggccacc aggccggccc gttccggacc tgggacctgc tcggcgtgcg
     1141 cgggacgacc gagcggatgc gccgcctcgg cctggaggtc gcgccctggg tggacgactt
     1201 cctcggcgcc ggcaacgagt cgttctaccg cgacggcgcc gcctaccagc cgtcgtccgg
     1261 gacctacgag ccggtccgca ccgaccccga cgcgatcgac cttgccgcgc tcaaggccga
     1321 gggccgcgag gtgctcggca acgactcggc cagcctcgtc gacctcggcg acggggtgct
     1381 ctgcctcgag ctgcacgcgc ccgcgagcgc ggtcgacgcc cgcgtgatcc agctcggcca
     1441 ccaggcgctc gccgagctcg aggggggcga ctggaagggc ctcgtgatcg gcggccgggc
     1501 ggcgaacttc tgcgtcggcg ccaacctggc cgaggtcggc atggccgcct accagcgcgc
     1561 ctacgaccag gtgaaggagg cgtccaggtc gctgcaggag ctgctcatgg ggttccgctt
     1621 cgcggccaag ccggtggtgg ccgcgcccca cgggcagacg ctcggcgccg ggtgcgagat
     1681 ctgcctccac gccgaccggg tcgtggccgc cctcgagacc tacatgggcc aggtcgagac
     1741 cgcggtcggc ctgatcccgg ccggcggcgg gtgcaaggag ctcgtccgcc gcatcgtctc
     1801 cccggcgatg cgggtccagg acgcgccgcc gctgccgttc gtgcagcggg tgttcgagac
     1861 gatcgcgctc gccaaggtcg ggacgagcgc ggtcgagtcc cgcgagctgg ggttcctcgc
     1921 cgacgacgac cggatcgtgc tgaacgccga ccacctgctg accgcggcca agcgggaggt
     1981 gctggagctg gcccccgggt accggccgcc tccccgcgag gcctccgtct tcgccgccgg
     2041 ctcgccaacc ctggccgcgc tcgagctcgg cgtccgcacc cagcagtggg gcggctacgc
     2101 gagcgggtac gacggggtgg tcgccaccca cctcgccagc gtgctctgcg gcggcgagct
     2161 gtcggcgccc cagtgggtga ccgaggacta cctgctcgag ctggaacgag aggcgttcgt
     2221 cgagctgttg cggaacgaga agaccatgga gcggatccag gccatgctca ccacccgcaa
     2281 gcccctccgg aactgagccg gaacggaaga ggagcaccca tgaaggaagc ggttttggta
     2341 tctggggcgc ggacggcggt cgggcgggcg cccaaggggg cgctcagggc ggcgcggccg
     2401 gacgacatgg ccgccgccgc gatcaccgag gcgatcaagc gcgccgacgg cctcgacccg
     2461 gccgaggtgg aggacgtcgt cctgggctgc gcgatccccg agggcaccca gggcatgaac
     2521 gtggcccggg tcgccgccct gcgggccggg ctgccggagt cggtgccagg gcagaccgtg
     2581 aaccggttct gctcctccgg gctgcagacg atcgccagcg cggccgagcg catcatggcc
     2641 gggttcgcga ccacgatcgt ggccggcggc accgagcaca tgtcctcgac cccggtcacg
     2701 tcggccaact tcgcgcccaa ccccgaggtc gtccgctcgt tccccgaggt ctacatgtcg
     2761 atgggcctca ccgcggagcg ggtcgccgag cgctggaagg tgtcaaggga ggaccaggac
     2821 gagttcgcgc tccgctcgca ccggcgcgcg gccgaggcgg tggactcggg ccgcttcgac
     2881 ccggagatcg tccccctgcc cgtcaccctc gactgggtcg acggggacga gcggcggcac
     2941 agcgagacca ccttcgcccg cgacgagggc ccgcgcc
//
LOCUS       DASPYY010000651         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_387955, whole genome shotgun sequence.
ACCESSION   DASPYY010000651 DASPYY010000000
VERSION     DASPYY010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387955"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1595
                     /locus_tag="VEP73_10260"
     CDS             192..1595
                     /locus_tag="VEP73_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HYT26851.1"
                     /translation="MSSLHERLARARTDGALPPVLPEEPETRRPHRARPDPLADLRQK
                     VHRSLVETLGPKLYDSQLSEEQLRTRVHDILQRTLEEEPTPLTRDERQRLVAEIADDV
                     LGFGPIEPFLRDPTVTEIMVNAPDVIYVEREGRLYPTEGRFVDETHLRRVIDKIVGQV
                     GRRIDESSPYVDARLPDGSRLNAVIPPLCVGGGPYLTVRKFSPDPYQADDLVTFGTMS
                     AKLSRLLDAAVHGRLNVLVSGGTGAGKTTTLNVLSSFIPPGDRIITIEDAAELQLRQP
                     HWLRLEYRPPNIEGRGEVTIRDLVRNALRMRPDRIVVGEVRGGETLDMLQAMNTGHDG
                     SLTTVHANSPRDSLSRLETMVLMAGVDMPMRAIREQISSAINLIVHQARLKDGSRRVT
                     HVTEIVGMEGDVITLQDLFLFDYRAGIDENGRFRGVIQPTGLRPTFVDRLGDQGIRLD
                     PDLFLHEPGTIPIAMRR"
     gene            1642..3585
                     /locus_tag="VEP73_10265"
     CDS             1642..3585
                     /locus_tag="VEP73_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYT26852.1"
                     /translation="MSRRVLVAAALLALASAGSRPAAAESGRLDVRVTQLEATGPASA
                     RATVAVSGPAVEGRQLTAGAFTATVGGGPAPVSGVTPPSAARTPVSVLLVIDTSGSMG
                     PSGDIDRAKAAADRFVDQLRPGGRIGVVRFSNQPATVRPLTGDASAAKASIATLRANG
                     DTALNDAVVAAVDQLADAPGQRVVVLLSDGADNRSTHTVEDAITAAKRAKAVVYTVGL
                     DNGLTPAPVLTRLAAETRGKFVKAQGARLADLYASLGRELYSQYVVDFRVPPGTPGET
                     QVVVTVAAGGARGSSEGRVLLLPASPSQARGLDQQPVTGLTRLETRQGLYLVAALMFV
                     AVLLLGYALMSTPPGSGRSYRELRQRLSQYSLTPTIDDEARAGGFGSGDLVGKATELA
                     ESLVRRGNLEEAFLSRIELAGLKLRVAEFVLISLASAFGFPLLLLLLTRNLLVTVAGV
                     AVGVLAPFVFLVIRAARRQAKFEEQLPDTLQLLGGALQAGHSLLQAVDTVVKEADEPI
                     SVEFQRVLTEARLGMPLEEALDGVAKRMNSQDFEWTVMAVSLQRQIGGNLAELLNTVA
                     STIRERYALKRQIRSLSAEGRLSSIILSILPFLMFFILLTFNPTFLAPLYTTPLGLAM
                     LSASAILMIVGILWMKKITDIEV"
     gene            3590..>3857
                     /locus_tag="VEP73_10270"
     CDS             3590..>3857
                     /locus_tag="VEP73_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26853.1"
                     /translation="MSAMLLVAGATALAGAVFAALFSVQSLVVERRQSYRTIRALRAI
                     DLKPTDIRNRELAEPARNRVWAPLYRAALAVARRLTPLGAKQNIQ"
BASE COUNT          556 a         1460 c         1319 g          522 t
ORIGIN      
        1 ggaggatccc aagggccacg tgagcgaggc catccaacgg gtcgcggccc agttcgcgga
       61 cggccagcgg gcctcgcgcg ccggacgggc caagccgcgc cgctccctct tctcgcgacc
      121 ctgagcgcca ccgaccgagc gccaccgacc ggccgcccgc ggccgaccga ccgccaagga
      181 gcacacgacc gatgtcctct ctgcacgagc ggctggcccg ggccaggacg gacggggcgc
      241 tgccgcccgt cctgcccgag gagccggaga cccgccggcc ccaccgggca aggcccgacc
      301 cgctggccga cctccgccag aaggtgcacc ggtccctggt cgagaccctc ggcccgaagc
      361 tctacgactc gcagctcagc gaggagcagc tccggaccag ggtccacgac atcctccagc
      421 ggacgctcga ggaggagccg acccccctca cccgggacga gcggcagcgg ctcgtcgccg
      481 agatcgccga cgacgtcctc ggcttcggcc cgatcgagcc gttcctgcgc gaccccaccg
      541 tcaccgagat catggtcaac gcgcccgacg tcatctacgt cgagcgcgag ggcaggctgt
      601 acccgaccga gggccgcttc gtggacgaga cccacctgcg ccgggtgatc gacaagatcg
      661 tcggccaggt cgggcgccgg atcgacgaga gctcgcccta cgtcgacgcc cgcctgcctg
      721 acggctcccg gctgaacgcg gtgatcccgc cgctgtgcgt cggcggcggc ccctacctga
      781 ccgtccgcaa gttctcgcca gacccctacc aggccgacga cctggtcacc ttcggcacga
      841 tgtcggccaa gctgtcgcgg ctgctcgacg ccgccgtcca tggacggctg aacgtgctgg
      901 tgtccggggg gacgggtgcg ggcaagacga ccaccctcaa cgtgctgtcg tcgttcatcc
      961 cgccgggcga ccgcatcatc accatcgagg acgccgccga gctgcagctc cgccagcccc
     1021 actggctccg gctcgagtac cggccgccca acatcgaggg ccggggcgag gtcaccatcc
     1081 gggacctggt gcgcaacgcg ctgcgcatgc ggcccgaccg gatcgtcgtc ggcgaggtcc
     1141 gcggcggcga gaccctggac atgctgcagg ccatgaacac cggccacgac ggctcgctga
     1201 cgaccgtgca cgccaactcg ccgagggact cgctgtcccg gctcgagacc atggtgctca
     1261 tggccggggt cgacatgccc atgcgggcca tccgcgagca gatctcctcc gcgatcaacc
     1321 tcatcgtgca ccaggcccgc ctcaaggacg gcagccgccg ggtgacccac gtgaccgaga
     1381 tcgtcgggat ggaaggcgac gtcatcaccc tgcaggacct cttcctgttc gactaccggg
     1441 ccggcatcga cgagaacggc cgcttccggg gcgtcatcca gccgaccggg ctgcgtccga
     1501 ccttcgtcga ccggctgggc gaccagggca tccgtctcga cccggacctg ttcctccacg
     1561 agccaggcac gatcccgatc gccatgcgcc gatgagcacg gcgggcgcac ccggagccct
     1621 tcgtggcagg agggagcgcc gatgagcagg cgcgtgctcg tggcggcggc gctgctcgca
     1681 ctcgcctcgg ccggctcgag gccggcggcg gccgagtcgg ggcggctcga cgtccgggtg
     1741 acgcagctcg aggccacggg cccggcctcg gcgcgggcca ccgtcgcggt ctccgggccc
     1801 gcggtcgagg gcaggcagct caccgccggc gcgttcaccg cgaccgtcgg cggcgggccc
     1861 gcgccggtca gcggggtcac gccgcccagc gccgcgagga cgccggtgtc ggtcctcctc
     1921 gtcatcgaca cgagcggcag catgggcccg agcggagaca tcgaccgcgc caaggcggcg
     1981 gccgaccggt tcgtggacca gctccggccc ggaggccgga tcggggtggt gcggttctcc
     2041 aaccagcccg cgaccgtccg ccccctcacc ggggacgcga gcgccgcgaa ggcgagcatc
     2101 gcgacgctgc gggccaacgg cgacaccgcg ctcaacgacg cggtcgtcgc ggccgtcgac
     2161 cagctcgcgg acgcgcccgg ccagcgcgtc gtcgtgctgc tgtccgacgg cgcggacaac
     2221 cggtccaccc acaccgtcga ggacgcgatc accgccgcca agcgcgcgaa ggccgtcgtc
     2281 tacacggtcg ggctggacaa cgggctgacc cccgcgccgg tcctgacgag gctcgccgcg
     2341 gagaccaggg ggaagttcgt caaggcccag ggcgcgcggc tggccgacct ctacgcgagc
     2401 ctcggcaggg agctctactc acagtacgtg gtcgacttcc gcgtgccgcc tggtaccccg
     2461 ggcgagaccc aggtcgtcgt cacggtcgcc gcgggcggcg cccgggggtc gtcagagggg
     2521 cgcgtcctgc tgctgccggc gtcccccagc caggcacgcg gcctggacca gcagccggtc
     2581 accgggctga ccaggctcga gaccaggcag gggctctacc tggtcgcggc cctcatgttc
     2641 gtcgccgtcc tcctgctcgg ctacgccctc atgtcgacgc cgcctggcag cggcaggagc
     2701 taccgggagc tgcggcagcg gctgtcgcag tactcgctca cgcccaccat cgacgacgag
     2761 gcccgggcgg gcgggttcgg ctcgggcgac ctcgtcggca aggcgaccga gctggccgag
     2821 tcgctggtca ggcggggcaa cctcgaggag gcgttcctca gccggatcga gctggccggc
     2881 ctgaagctcc gcgtggccga gttcgtcctg atctccctgg cgtcggcctt cggcttcccc
     2941 ctcctgctcc tgctgctgac gaggaacctc ctggtcaccg tcgccggcgt ggcggtcggc
     3001 gtgctcgcgc cgttcgtgtt cctcgtgatc agggccgcgc gccgccaggc gaagttcgag
     3061 gagcagctgc ccgacacctt gcagctgctc ggcggcgcgc tccaggccgg ccacagcctc
     3121 cttcaggccg tcgacacggt ggtcaaggag gccgacgagc cgatttcggt agaattccag
     3181 cgcgtgctga ccgaagcgcg gctcgggatg cccctcgagg aggctctcga tggtgtggcc
     3241 aagcgcatga acagccagga cttcgagtgg acggtcatgg cggtgagcct gcagcggcag
     3301 atcgggggca acctggccga gctgctgaac accgtcgcct cgacgatccg ggagcgctac
     3361 gcgctcaagc gccagatccg ttccctctcg gctgaaggcc ggctgtccag catcatcctg
     3421 tcgatcctac cgttcttgat gttcttcatc ctgctgacgt tcaatccgac cttcctggcc
     3481 ccgctctaca cgacgcccct cggactcgcc atgctgtccg cgtccgcgat cctgatgatc
     3541 gtcggcatcc tctggatgaa gaagatcacc gacatcgagg tgtagcgcca tgtcagccat
     3601 gctgctcgtc gccggtgcca ccgccctcgc cggggcggtg ttcgcggcgc tgttctccgt
     3661 ccagtccctg gtcgtcgagc ggcgccagtc ctaccggacg atcagggcgc tgcgggcgat
     3721 cgacctgaag ccgaccgaca tccgcaaccg cgagctggcg gagcccgcca ggaaccgcgt
     3781 ctgggcgccg ctctaccgcg ccgccctcgc cgtcgcgcgg cgcctcacgc cgctcggcgc
     3841 gaagcagaac atccagc
//
LOCUS       DASPYY010000652         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2496683, whole genome shotgun sequence.
ACCESSION   DASPYY010000652 DASPYY010000000
VERSION     DASPYY010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496683"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VEP73_10275"
     CDS             <1..745
                     /locus_tag="VEP73_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010596024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26854.1"
                     /translation="LGLGLSTFFVRETRGHAHHEATNTAAGVHGADLPTRRVAALVSY
                     REPALASAAQAGMVNNLNDGLAWGLFPLLFATAGLTLGQIGVLAALYPAVWGLGQLAT
                     GPLSDRHGRKPFIVVGMLLQAVALGWVAAAGSFTAWAAAAVLLGAGTAMVYPTLLATI
                     GDVAHPAWRARAVGVYRLWRDGGFAVGALLAGLLADAYGIRTATAVVAALTAASGVLA
                     WIRMYETHPRPRREPAKANSTTPRTEPTA"
     gene            complement(822..1703)
                     /locus_tag="VEP73_10280"
     CDS             complement(822..1703)
                     /locus_tag="VEP73_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYT26855.1"
                     /translation="MDGVSFEQLVAQVDQTTASDDPLDRVDAGVTVAAALAGHADSLV
                     DHVVAQARTAGRSWTEIGARLGVSKQAARKRFTDPTAPTPTPVLPPEVTLRPRLRSCL
                     TRANQLAQAAGAAQVGAEHLLAGLLTDGVAAAILDKLAVTADAITTSTLRLFGPANRP
                     TDTVPPLSADAVCAIEAAAQHAQVRADNPDHVTVGTEHLLAVLALDHGSRAHRILVDL
                     GVDIAAVKKELACYLTLNPPRPRRPRRNRRTPTVACSFCGTPESPNRPLAHGPGVTIC
                     TVCAQRAAQSLAVRAAS"
BASE COUNT          212 a          632 c          669 g          256 t
ORIGIN      
        1 cctcggcctc ggcctgtcca ccttcttcgt ccgggaaacc cgcggccacg cccaccacga
       61 agccaccaac acggcggccg gcgtccacgg cgccgacctg cccacccgcc gcgtcgccgc
      121 gctggtcagc taccgcgaac ccgccctcgc ctcggcggcg caggccggca tggtgaacaa
      181 cctcaatgac gggctggcct ggggtctgtt ccccctgctg ttcgccaccg ccgggctcac
      241 cctcggccag atcggcgtac tcgccgcgct ctaccccgcc gtgtggggcc tcggtcaact
      301 cgccaccgga ccactgtccg accggcacgg ccgcaagccg ttcatcgtcg tcgggatgct
      361 gctgcaggcg gtcgccctcg ggtgggtcgc cgccgccggc tcgttcaccg cctgggcggc
      421 cgccgcggtg ctgctcggcg ccggcaccgc catggtctac ccgaccctgc tcgccaccat
      481 cggcgacgtc gcccaccccg cctggcgcgc ccgcgccgtc ggcgtctacc ggctgtggcg
      541 cgacggcggc ttcgccgtcg gcgcgctgct cgccggcctc ctcgccgacg cctacggcat
      601 ccgcaccgcc accgccgtcg tcgccgccct caccgccgcc tccggggtcc tcgcctggat
      661 ccgcatgtac gagacacatc cgcgcccgcg gcgcgagccg gcaaaggcga acagcacgac
      721 cccaagaacc gaaccgaccg cctagtcaca ggtgttccgg ctctcatacc gggccgggag
      781 gcggcacgcc cccgccgcgc cactgcggcc gacccgatcg gtcaggacgc ggcgcgtacg
      841 gcgaggctct gggcggcgcg ttgggcgcag acggtgcaga tcgtgacgcc agggccgtgt
      901 gccagtggtc ggttgggcga ttcgggggtg ccgcagaacg agcaggcgac ggtcggtgtc
      961 cgtcggttgc ggcgcgggcg ccgggggcgt ggcgggttga gggtgaggta gcaggcgagt
     1021 tccttcttga ccgcggcgat gtcgacgccg aggtcgacca ggatgcggtg ggcgcgggag
     1081 ccgtggtcga gggcgaggac ggcgaggagg tgttcggtgc cgaccgtgac gtggtcgggg
     1141 ttgtcggcgc ggacctgggc gtgttgggcg gcggcttcga tcgcgcagac cgcgtcggcg
     1201 gacagcggcg gcacagtgtc ggttggtcgg ttggcgggac cgaacagccg cagggtggat
     1261 gtggtgatcg cgtcggcggt gacggcgagt ttgtcgagga tcgccgcggc gaccccgtcg
     1321 gtgagcagcc cggcgagcag gtgctcggcg ccgacctgtg ccgcgccggc cgcttgggcg
     1381 agttggttgg cgcgggtcag gcagcttcgc agccggggcc gcagggtgac ctccggcggc
     1441 aggaccgggg tgggtgtggg cgcggtgggg tcagtgaagc gtttgcgtgc ggcctgtttg
     1501 ctgaccccga gtcgggcgcc gatctcggtc caggaccggc ccgccgtacg ggcctgggcg
     1561 acgacgtggt cgacgagcga gtcggcgtgg ccggccaggg ccgccgcgac ggtcactccg
     1621 gcatcgaccc ggtccagcgg gtcgtcgctg gcggtggtct ggtcgacctg ggcgacgagc
     1681 tgctcgaacg acaccccatc catgcgacaa ccataaattg tcgacaccgg ggagacaacc
     1741 acgggttgtc accccgtcct ggtgtggtc
//
LOCUS       DASPYY010000653         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1514394, whole genome shotgun sequence.
ACCESSION   DASPYY010000653 DASPYY010000000
VERSION     DASPYY010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514394"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..837)
                     /locus_tag="VEP73_10285"
     CDS             complement(151..837)
                     /locus_tag="VEP73_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT26856.1"
                     /translation="MRILVVEDEEKMARAVRRGLEQEGYAVDVAADGEEGLFQGTEHA
                     YDAIVLDVMLPGLNGLEVCERLRAAGRWAPVLMLTARDAVPDRIGGLDAGADDYLVKP
                     FAFGELLARLRALVRRCPRERPVVLTVGDLQLDPAAHTVTRAGQPVDLSAREFALLEY
                     LMRNAGEVVTRTRILDHVWDYDYDGFSNVIDVYIGYLRRKLDGDGRPQRIRTVRGVGY
                     LLVPQEPASP"
     gene            953..>1646
                     /locus_tag="VEP73_10290"
     CDS             953..>1646
                     /locus_tag="VEP73_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26857.1"
                     /translation="MSVDVEPQPVLGRPGFPGRPSGSARAASAIALLAGLLNLASAAL
                     PAERSRLALLDAMVPGAVSSGAAVWTAAAGVGLILLAGGLRRHRRTAWVATLGLLAGS
                     AVSHLVKGLDVEEALVEAFLAGLLTAKGDRFTAHPGPGERAPVVRPAIAVAVTTLTFG
                     VAGLLVNRGDVAEDLPLRVLVVQAARMAAGFGTSLTVTGRFWRAFPGSVAAVFYLGAA
                     LVLAQALAPARCR"
BASE COUNT          175 a          621 c          634 g          216 t
ORIGIN      
        1 cgtgcccggc gcgcccttcc gccgcggccg gcgccgtcca ggggcccacc gcgagcgccg
       61 cgacgaccag ggcggcaccc agggcggccg tggtgcgcgg gcgcggggca tgcccctggc
      121 cggcgcctgg gacgggcagg cagccggcgg tcacgggctg gccggctcct ggggcaccag
      181 gaggtagccg acgccccgca cggtgcggat gcgctgcggg cggccgtccc cgtcgagctt
      241 gcgccgcagg tagccgatgt agacgtcgat gacgttggag aacccgtcgt agtcgtagtc
      301 ccacacgtgg tcgaggatgc gggtgcgggt cacgacctcg ccggcgttgc gcatgaggta
      361 ctcgagcagg gcgaactcgc gggccgacag gtcgaccggc tgcccggccc tggtcaccgt
      421 gtgggcggcc gggtccagct ggaggtcgcc gacggtgagc accaccgggc gctcgcgcgg
      481 gcagcgcctg accagcgccc gcagccgcgc gagcagctcg ccgaacgcga agggcttgac
      541 gaggtagtcg tcggcgcccg cgtccaggcc gccgatccgg tcgggcaccg cgtccctggc
      601 cgtcagcatg agcaccggcg cccagcggcc ggccgcgcgc agccgctcgc agacctcgag
      661 cccgttgagg ccgggcagca tgacgtcgag gacgatcgcg tcgtaggcgt gctccgtccc
      721 ctggaacagc ccctcctcgc cgtcggcggc gacgtcgacg gcgtacccct cctgctcgag
      781 cccgcgccgg accgcgcggg ccatcttctc ctcgtcctcg accaccagga tgcgcatgcc
      841 gatccctcca tgccgccagt gaaccgcccg ggcgatgaga ccctcatgag agcccgccgg
      901 ggcggcgtga ctctcatgag ggtctcatcc cggggggagt acgacgtcca ccgtgagcgt
      961 ggacgtcgag ccgcagccag tgctcgggcg gccgggattt cccggccgcc cgagcgggtc
     1021 tgcccgggcc gcctcggcga tcgcgctgct cgccgggctg ctgaacctgg cgtcggccgc
     1081 gctgccggcc gagcggagcc gcctcgccct gctggacgcg atggtccccg gggcggtgtc
     1141 gagcggggcg gcggtgtgga ccgccgccgc cggcgtcggc ctgatcctgc tggccggcgg
     1201 gctccgccgg caccgccgca cggcttgggt ggcgaccctc gggctgctcg cgggcagcgc
     1261 cgtgtcgcac ctcgtcaagg gcctcgacgt ggaggaggcc ctcgtcgagg cgttcctggc
     1321 cgggttgctg accgccaaag gggaccggtt caccgcccat cccggccccg gcgagcgcgc
     1381 cccggtcgtc cggccggcca tcgcggtggc cgtgaccacg ctgacgttcg gtgtggcggg
     1441 gctgctcgtc aaccgcggcg acgtcgccga ggacctgccg ctgcgggtgc tcgtcgtcca
     1501 ggcggcccgg atggcggccg gcttcggcac ctcgctcacg gtcacgggcc gcttctggcg
     1561 cgccttcccc gggtcggtgg cggccgtgtt ctacctcggg gcggcgctcg tgctcgcgca
     1621 ggcactggcg cccgcgcggt gccggc
//
LOCUS       DASPYY010000654         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_249630, whole genome shotgun sequence.
ACCESSION   DASPYY010000654 DASPYY010000000
VERSION     DASPYY010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249630"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VEP73_10295"
     CDS             complement(<1..1057)
                     /locus_tag="VEP73_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HYT26858.1"
                     /translation="MADSRPLAGRYRAVVCDLDGVVYLRDAVVPAAPAALRGVRALGV
                     AVAFLTNNSLRPPEDVAAKLVRMGVGAAPGEVLTSSEAVVHLLGGKAALAGARVLVVG
                     GPGLRQALLGAGADLAGPSSWREAEIVVVGIDPEVTYEKLATACLAIRAGARFVGSNP
                     DTTLPVPEGSVPGNGAFLALLETATGVRPQVAGKPEPALFETAAAAVGPGPYLMVGDR
                     ADTDLEGAARLGWDTALVLTGVVRPDGLLDLPRAPGHLLADVGGLLAPPGPSILEAAG
                     HARGAGGDGEAEAAAALLRDVGLPGVPEAGAGGRRQAPGLDRSPLLVAVDGDRVVGAV
                     AWERHGDVALLRGVAVAG"
     gene            complement(1050..1472)
                     /locus_tag="VEP73_10300"
     CDS             complement(1050..1472)
                     /locus_tag="VEP73_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT26859.1"
                     /translation="MIAVEADAYELFRKGIELLAGHHPHQAATVLERARALAPRQGSV
                     SEALGRAYYEAGRPADAQVQFARVLELDPTNDYAHFGLALCLSRAGERVLALGHLRLA
                     LAMRPGVAAYEEALARVERAVPGAGALPPQPGDDPGRG"
     gene            1721..>2809
                     /locus_tag="VEP73_10305"
     CDS             1721..>2809
                     /locus_tag="VEP73_10305"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HYT26860.1"
                     /translation="MVAAAPFRGLRFDPAAVGDPGAVTAPPYDAIPPEARDAYEARSP
                     YNVVRLILPRGVPGDPARYGHVAGLLDAWRDDGALTLEPVPALYLYEEAYTVGGEPRV
                     QRGVLATVTLDDSGTTILPHERTMAGPVQDRLRLLEVSRANLSPLFGVYAGGGRAAAV
                     LDDLTGTPPATACVDEAGVRHRLWTVTDPDRIEAWCGRLADHRVLIADGHHRYQASLL
                     YRDAMRDAGPGRRVRDAGPDPRPAPWDEVLMYLVDADQQGPSIRPVHRLLTDLTAPAV
                     AAALADEFDARAVPRVDDLEAALAALPAASVAFGLYGDGRSRVLVARDPGLLAAEAGS
                     DHPPLDVEVLHGPVLAKRLGVTDFEGRVL"
BASE COUNT          345 a         1140 c         1035 g          289 t
ORIGIN      
        1 cgcccgcgac cgcgacaccc cgcagcagcg cgacgtcgcc gtggcgctcc caggccaccg
       61 cgccgaccac ccggtcgccg tcgaccgcga cgagcagcgg ggagcgatcc aggcccggag
      121 cctgccgccg cccgcccgcg ccggcctcgg ggacgccggg caggccaacg tcgcgcagca
      181 gggccgcggc cgcctccgcc tcgccgtcgc cgccggcccc tcgggcgtgc ccggccgcct
      241 ccaggatcga aggtccgggg ggtgcgagca ggccgccgac gtcggccagc agatggccgg
      301 gcgccctggg caggtcgagc aggccgtccg gccgcacgac ccccgtgagc accagcgcgg
      361 tgtcccagcc gagccgggcc gcgccctcga ggtcggtgtc ggcccggtcc cccaccatga
      421 ggtacggccc ggggccgacc gcggccgcgg cggtctcgaa cagggcgggc tccggcttgc
      481 ccgccacctg gggccggaca ccggtggcgg tctcgagcag ggcgaggaac gcgccgttgc
      541 ctggcaccga cccctcgggg acgggcaggg tcgtgtccgg gttgctgccg acgaaccggg
      601 caccggctcg gatggcgagg caggcggttg ccagcttctc gtaggtcacc tcggggtcga
      661 tgccgaccac gacgatctcc gcctcccgcc aggacgacgg accggccagg tcggcaccgg
      721 cccccaggag ggcctggcgc agccccgggc cgccgacgac gagcaccctg gcaccggcca
      781 gcgccgcctt cccaccgagc aggtggacga ccgcctcgga cgaggtcagc acctcgccgg
      841 gcgcggcccc gacgcccatc ctcacgagct tcgcggccac gtcctccggc ggccgcaggg
      901 agttgttggt caagaaggcg accgccaccc cgagggcgcg gacgccccgc agcgccgcgg
      961 gcgcggccgg gaccaccgcg tcccgcaggt acacgacgcc gtccaggtcg cagacgaccg
     1021 cgcggtaccg cccggccagg gggcgggagt cagccacggc cggggtcgtc acccggttgc
     1081 ggcgggagcg cgcctgcgcc gggcacggcc cgctcgacgc gggcgagcgc ctcctcgtag
     1141 gcggccaccc ctggtcgcat cgccagcgcg agccgcaggt gcccgagggc gaggacccgc
     1201 tcgccggccc gggacaggca cagggcgagg ccgaaatggg cgtagtcgtt ggtcgggtcg
     1261 agctcgagca ccctggcgaa ctggacctgg gcgtccgccg ggcggccggc ctcgtagtac
     1321 gcgcgcccca gcgcctcgct caccgatccc tgccgcgggg caagggcgcg cgcccgctcc
     1381 agcacggtcg ccgcctgatg ggggtggtgg ccggcgagca gctcgatgcc cttgcggaac
     1441 agctcgtagg cgtcggcttc cacggcgatc acctccaagc accagggggg cggcatccgt
     1501 ccgcgcggcc ggccacaccg ggtccggacg aggagcgctc ggatggcggt acccgacaac
     1561 ggtacaccgg ccgaccagcc ggccggcgct gcaccggtcg gcagccggcg ggcaggacag
     1621 cggccgtccc cggcccggct ggcgggacgg gggcacccct tgctacaacg aacggcatga
     1681 ccgacgacgc accgacccgc gacgcaccgc cggccgcgcg gtggtagccg cggccccctt
     1741 ccggggcctg cggttcgacc cggccgcggt cggcgacccg ggagccgtga ccgcccctcc
     1801 ctacgacgcc atcccgcccg aggccaggga cgcctatgag gcgcggagcc cctacaacgt
     1861 cgtgcgcctg atcctcccaa ggggcgtccc cggcgacccg gcccggtacg ggcacgtcgc
     1921 cgggctgctc gacgcctggc gggacgacgg cgccctgacg ctcgagccgg tcccggcgct
     1981 ctacctgtac gaggaggcct ataccgtcgg gggcgagcca cgggtgcagc gcggcgtgct
     2041 cgccaccgtc accctggacg actcgggcac gaccatcctg ccccacgagc gcaccatggc
     2101 gggtccggtc caggaccggc tccgcctcct cgaggtgtcg agggccaacc tgtcgcccct
     2161 gttcggggtc tacgcgggcg gaggccgggc cgccgcggtg ctcgacgacc tcaccggcac
     2221 gcccccggcg accgcctgcg tcgacgaggc gggggtgcgg caccgcctgt ggacggtcac
     2281 cgacccggac cggatcgagg cctggtgcgg ccggctcgcg gaccaccggg tgctgatcgc
     2341 cgacggccac caccgctacc aggcctcact cctctaccgg gacgccatgc gggacgccgg
     2401 gccgggccgc cgggtgcggg acgccgggcc ggatccacgg cccgccccct gggacgaggt
     2461 gctcatgtac ctcgtcgacg ccgaccagca ggggccgtcc atcaggcccg tgcaccgcct
     2521 gctcaccgac ctcaccgccc cggccgtcgc cgccgccctg gccgacgagt tcgacgcccg
     2581 ggccgttccc agggtcgacg acctcgaggc ggccctcgcg gcgctcccgg ccgcctccgt
     2641 cgccttcggc ctctacggcg acggccggag ccgcgtcctg gtcgcccgcg accccggcct
     2701 cctcgccgcc gaggccgggt ccgaccatcc cccgctcgac gtcgaggtgc tgcacgggcc
     2761 ggtcctggcc aagcgcctcg gcgtgaccga cttcgagggc cgcgtcctg
//
LOCUS       DASPYY010000655         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2497535, whole genome shotgun sequence.
ACCESSION   DASPYY010000655 DASPYY010000000
VERSION     DASPYY010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497535"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VEP73_10310"
     CDS             <1..761
                     /locus_tag="VEP73_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYT26861.1"
                     /translation="AVSDHMGDQLGSIAYFDSYPADLAVLGELSDNEIYLGHRGRCYF
                     DITVLGKSAHTCHKPLAVNANTLAAHAVLELDASHLTPRLDQWVTDLFGAETYMVPGR
                     IYGGLAPGGPSMIPDECVIRVDCRPQPGVTTDQVRAEIDRCLAAAARSDPRFRAEVEL
                     ADVKDGYLARRDDEVVTLMVDAVRRVRGREPVLQAASWLGDTASFGAKVPTVIFGPGG
                     EPVYCPDEHLSVADIVEATQVYAAFGALALTPRA"
     gene            764..1822
                     /locus_tag="VEP73_10315"
     CDS             764..1822
                     /locus_tag="VEP73_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT26862.1"
                     /translation="MPPALGSRPATAPSFEALLQRPGLAWMGQNTTHLEPPPEVVAAL
                     LDSTRRHEFQFYAPALGFDELRELIVADLGLADSAQIAMITDGAVGGLHHICTALAPG
                     LSRLITTDPGWPWPGRFVRLQGVPVTAVEVYSDQAGYRLTAAALAEVIEPRSLLYLID
                     PLNPLGSSYRRDELAALVDLARANDAIVVHDCTYRHFADGHTLAADLYPEGTLTTYSF
                     SKWLGLAGLRLGANISAQRAAIAGLKVKDSWLRELRRVNRRNQQIVTEAVEHGPFGRV
                     LVQPSQGNFLAVDVGGHGWTAGQVGAAMLDRDVFIRSGTYQSSAFGDRFVKVSTSVPT
                     AWAERFAAAWARLGDRPS"
     gene            1819..>2932
                     /locus_tag="VEP73_10320"
     CDS             1819..>2932
                     /locus_tag="VEP73_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYT26863.1"
                     /translation="MTGLARFAGLGVEEYQASTALELGRRVADGSLSPVQLTECALHL
                     AAEAEPVINAYAALLPERARREAAVREAQARAGLIRGPLHGVPVAVKDNFYYQGEPTG
                     KGSLTSSDEPARESAPMVARLTSAGAIVIGKTTTPEFGWKGTGISPRTGVTRNPWNPA
                     RNSGGSSAGSGATVASGAVPVATGTDAGGSIRIPASFCGVVGFKPTLGAIPVWPGTVN
                     ETLSHAGPLTRSVADARAVFELTRGPDARDPQSAFPAPLADPPGRRLRVGVVREPFGV
                     APDPSVAAVFGRTVALIRSTAGTELTEVAALAGPPPRDVFEAMWVTGRGLGFADLIRR
                     HGDVMDPGLTRLLPLAERYSLADYYRALERRRAFNAR"
BASE COUNT          372 a         1108 c         1054 g          398 t
ORIGIN      
        1 cggccgtctc cgaccacatg ggcgaccagc ttggctcgat cgcgtacttc gactcttacc
       61 cggccgacct cgccgtgctc ggcgagctgt ccgacaacga gatctacctt ggccaccggg
      121 ggcgctgcta cttcgacatc acggtgctcg gcaagtcggc gcacacctgc cacaagccgc
      181 tggccgtcaa cgccaacacc ctcgccgcgc acgccgtcct cgagctcgac gcctcgcacc
      241 tcaccccgcg gctcgaccag tgggtgaccg acctgttcgg ggcggagacc tacatggtgc
      301 ccggccggat ctacggcggc ctggcgccgg gcgggccctc gatgatcccg gacgagtgcg
      361 tgatccgggt cgactgccgg ccccagccgg gcgtgaccac cgatcaggtc cgggccgaga
      421 tcgaccggtg cctggccgcc gccgcccgga gcgacccgcg cttccgggcc gaggtcgagc
      481 tggccgacgt caaggacggc tacctggccc gccgcgacga cgaggtcgtg acgctcatgg
      541 tggacgcggt ccgccgggtc cgcggccgcg agccggtcct gcaggcggcc agctggctcg
      601 gcgacacggc cagcttcggc gcaaaggtgc ccacggtcat cttcggcccg ggaggcgagc
      661 cggtgtactg cccggacgag cacctcagcg tcgccgacat cgtcgaggcc acccaggttt
      721 acgcggcctt cggcgcgctg gccctgaccc cccgggcgtg acgatgccgc ccgcgctcgg
      781 gagccggccc gccacggcgc cgtcgttcga ggccctcctg cagcgcccgg gcctggcctg
      841 gatgggccag aacaccacgc acctcgagcc gcctcccgag gtggtcgcgg cgctgctgga
      901 cagcacccgg cggcacgagt tccagttcta cgcgcccgcc ctcggcttcg acgagctccg
      961 cgagctgatc gtggccgacc tgggcctggc cgacagcgcc cagatcgcga tgatcaccga
     1021 cggcgcggtc ggcgggctgc accatatctg caccgcgctc gcgcccgggc tgtcccggct
     1081 catcaccacc gaccccggct ggccgtggcc gggccgcttc gtgcggctgc agggcgtccc
     1141 ggtgaccgcg gtcgaggtgt actccgacca agccgggtac aggctcaccg cggccgcgct
     1201 ggccgaggtc atcgagccgc gatcgctgct ctacctgatc gatccgctca acccgctggg
     1261 cagctcgtac cgccgggacg agctcgcggc cctggtcgac ctggcccggg ccaacgacgc
     1321 gatcgtggtg cacgactgca cctaccggca cttcgccgac gggcacacgc tggcggccga
     1381 cctgtatccc gagggcacgc tgaccacgta cagcttctcg aagtggctcg gcctggcggg
     1441 tctgcggctc ggcgcgaaca tctcggccca gcgggccgct atcgccgggc tgaaggtaaa
     1501 agactcctgg ctgcgtgagc tgcggcgggt caaccgaagg aaccagcaga tcgttacgga
     1561 agccgtcgag cacggcccgt tcggccgtgt gcttgtccag ccgtcgcagg gcaacttcct
     1621 cgccgtcgac gtcggcgggc acggctggac cgccggccag gtgggcgcgg ccatgctgga
     1681 tcgcgacgtc ttcatccgtt cgggcacgta ccagtcgtcg gcgttcgggg acaggttcgt
     1741 caaggtcagc acgtcggtgc cgaccgcctg ggcggagcgg ttcgccgccg catgggccag
     1801 gctcggggac cggccgtcgt gaccgggctg gccaggttcg ccgggctcgg cgtcgaggag
     1861 taccaggcct cgacggcgct cgagctcggc cggcgggtgg ccgacggctc gctctccccg
     1921 gtgcagctga ccgagtgcgc gctgcacctc gcggccgagg ccgagccggt catcaacgcc
     1981 tacgccgcgc tgctgcccga acgcgcgcgg cgcgaggcgg ccgtgcgcga ggcgcaggcc
     2041 cgcgccgggc tcatccgcgg tccgctgcac ggggtcccgg tcgcggtgaa ggacaacttc
     2101 tactaccagg gcgagccgac cgggaagggc tcgctcacca gctccgatga gccggcccgc
     2161 gaatccgcgc cgatggtggc ccggctgacc tcggccgggg cgatcgtgat cgggaagacc
     2221 accacgccgg agttcggctg gaagggcacg ggtatctcgc cgcgcaccgg cgtgacccgc
     2281 aacccgtgga acccggccag gaactccggc ggctcgagcg cgggctccgg cgccacggtc
     2341 gcgtccggtg cggtgccggt cgcgacgggg accgacgccg gcggctcgat ccgcatcccg
     2401 gcgtcgttct gcggcgtggt cgggttcaag ccgacgctcg gcgcgattcc ggtctggccc
     2461 ggcacggtca acgagacgct gtcccacgcc gggccgctga cccgttcggt cgccgacgcc
     2521 cgggccgtct tcgagctgac ccgcggcccg gacgcccgcg acccgcagtc cgcgttcccg
     2581 gccccgctgg ccgacccgcc cgggcggcgg ctgcgggtcg gggtggtgcg cgagccgttc
     2641 ggggtggcac cggacccgtc cgtagccgcc gttttcggcc gaacggtcgc gctgatccgc
     2701 tccaccgccg gcaccgagct caccgaggtg gccgcgctgg ccgggccgcc gccccgggac
     2761 gtcttcgagg cgatgtgggt gaccggccgc gggctggggt tcgccgacct catccgccgc
     2821 cacggcgacg tcatggaccc gggcctgacc cgcctgctgc cgctggccga acggtacagc
     2881 ctggccgact actaccgggc gctggagcgg cggcgggcgt tcaacgcacg ga
//
LOCUS       DASPYY010000656         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_249720, whole genome shotgun sequence.
ACCESSION   DASPYY010000656 DASPYY010000000
VERSION     DASPYY010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249720"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEP73_10325"
     CDS             complement(<1..374)
                     /locus_tag="VEP73_10325"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYT26864.1"
                     /translation="MRLYLLGRGIQHSQSPGLWNGVFGQLGLDWHYGLLDTGEDGLPR
                     ALERLRDPEVLGYNVTMPYKGWAYERSVIRNLDVQRARGCNWLRMQDGQLAADNTDVE
                     GARALLGLVPPVDRALLFGAGGT"
     gene            complement(385..1161)
                     /gene="pcaD"
                     /locus_tag="VEP73_10330"
     CDS             complement(385..1161)
                     /gene="pcaD"
                     /locus_tag="VEP73_10330"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HYT26865.1"
                     /translation="MSTCDVHYRFDGRAGAPVLLLSNSLGSTLDMWAPQVEALAERFR
                     VLRYDHRGHGRSPVPPGPWRLADLGHDVLALLDRLSIQRASFCGVSLGGAVGMWVAAH
                     APARVGRLALLCTAARFAPASAWAERARTVRDHGAGAVAGAVVARWFTPGFAAREPEL
                     VARMRAMIASTPARGYAACCEVLEHLDLRDDLAAIGAPTLVVAGRDDPATPPAQGRHL
                     AERIAGARLVVVADAAHLANVEQAATVTALLLDHLGRKRR"
     gene            1345..1896
                     /locus_tag="VEP73_10335"
     CDS             1345..1896
                     /locus_tag="VEP73_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26866.1"
                     /translation="MPAGAGQPGELAARPGPAASVDEGEFDLDIRLVPAGGPSSGRRR
                     PARAAWYDTDACGDTEACPPATEQGCDTAIQCDTVLNCDTDLDCETVAECDTVVDCAD
                     TDLDCADTQLDCADTRRDCAGDTMVGCPGDTEVGCPGDTIVGCPGDTYVDCPVPTDLA
                     TNCCEETDNTCASCYTDCRMCVP"
     gene            complement(1956..3149)
                     /locus_tag="VEP73_10340"
     CDS             complement(1956..3149)
                     /locus_tag="VEP73_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HYT26867.1"
                     /translation="MSDELATLDETLDADELLVCARTLVRAASENPPGDERAAAAAVR
                     PWLDDLGCQVRAVEPAPGRVSLVGLFDSLRPGPTLAVNGHLDVVPAGPADQWEHPPYG
                     AVVADGVLHGRGSLDMKGPIAAALCAARMLLDSGLPWTGRLVFQLVADEETVGTHGTR
                     ALLAAGLLEADGALVAEPTGMRLATAERGASWYVLRTLGRAAHGARPAEGVSAIAAMA
                     RLIPAVLGMTWDRTHPLLGGPTVNVGTIRGGDKVNMVPAWCEAEVDRRTIPGETAEQV
                     LDEFQAILDREAAADPSFRAELGGQGFSPASEVDPDAAVVRAAVAALADAGLPAEPVG
                     FGGATDARILNLEAGVPAIVLGPGDLALAHSTAERVPVADLLTAARVYARLYATFLCP
                     ELRPG"
     gene            3307..3777
                     /locus_tag="VEP73_10345"
     CDS             3307..3777
                     /locus_tag="VEP73_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYT26868.1"
                     /translation="MAGDGSDDRLVPVRHAEACLRNPRELLRTARVIAVVGLSPNPDR
                     PSNQVAWYLHHQGYRLYGVRPAAAGSDIFGVPVVASLDEVPEPIDVVDVFRRAPDTPD
                     VARAAVAVGARALWLQLGIANAETRAIAEAGGLVYVEDRCLKVEHLRLLGARTG"
     gene            complement(3793..>4324)
                     /locus_tag="VEP73_10350"
     CDS             complement(3793..>4324)
                     /locus_tag="VEP73_10350"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYT26869.1"
                     /translation="ADVINDPSGLHDPGVADAVAEAGATLVVMHAGGPPRTRPHRPAY
                     ADVVAEVAAFLADRAAVARHRGVPPDLLLVDPGHDFHKNTFHSLELTRRLGELRRLGY
                     PLMVALSNKDFIGETLGLPLEERLEGSLAAAVASIMGGANVVRVHDVRATVRAVRMTE
                     AILGWHRPAVTRRGLA"
BASE COUNT          548 a         1618 c         1666 g          492 t
ORIGIN      
        1 gtcccgcccg cgccgaacag cagcgcgcgg tcgaccgggg ggaccagccc gagcagcgcc
       61 cgggcgccct cgacgtcggt gttgtccgcg gcgagctggc cgtcctgcat gcgcagccag
      121 ttgcagcctc gtgcccgctg gacgtcgagg ttgcggatca ccgagcgctc gtaggcccag
      181 cccttgtagg gcatggtcac gttgtacccg aggacctccg ggtccctgag gcgctcgagc
      241 gcccgcggaa ggccgtcctc gccggtgtcg agcagcccgt agtgccagtc cagcccgagc
      301 tgcccgaaca ccccgttcca cagccccggc gactgggagt gctggattcc tcggccgagc
      361 aggtacagac gcacgcaccg aactctaccg ccgcttgcgc ccgaggtggt ccaggaggag
      421 cgcggtgacg gtggccgcct gctccacgtt ggccaggtgg gccgcgtcgg cgacgaccac
      481 caggcgggcg ccggcgatcc gctcggcgag gtggcggccc tgggccggtg gcgtcgccgg
      541 gtcgtcgcgg cccgccacca ccagcgtcgg cgcgccgatg gcggccaggt cgtcccgcag
      601 gtccaggtgc tcgagcacct cgcagcaggc tgcgtaccct cgcgcggggg tcgacgcgat
      661 catggcccgc atgcgcgcca ccagctccgg ctcgcgggcg gcgaacccgg gcgtgaacca
      721 gcgcgcgacc acggcgccgg cgaccgcgcc ggcgccgtgg tcgcgcaccg tccgggcccg
      781 ctcggcccag gcgctggccg gagcgaaccg cgccgcggtg cacaagagcg ccagccggcc
      841 gacgcgcgcg ggcgcatggg cggcgaccca catgccgacc gcgccgccca gcgacacccc
      901 gcagaacgac gcccgctgga tcgacaggcg gtcgagcagg gcgagcacgt cgtggccgag
      961 gtcggcgagg cgccacgggc cgggcgggac gggggagcgg ccgtggccgc ggtggtcgta
     1021 gcgcagcacg cggaaccgct ccgccagcgc ctcgacctgg ggtgcccaca tgtccagggt
     1081 gctgccgagc gagttcgaca agagcagcac gggcgcgccc gcccgcccgt cgaaccggta
     1141 gtggacgtcg caggtcgaca cggccgccct cctcctccta cgaaggcgcg attctagccg
     1201 cggtcacacg ccccgggccg cacgggccct gtggccggac gaggccgacc cgggctcgcc
     1261 gccgggtccg ggcgttgaca tcctggtggg tgggcgccca ggattcccgc gacgtcagct
     1321 gctccgcggg agggccgcgc catcatgcca gcgggagccg gccagcccgg cgagctcgcg
     1381 gcccgacccg ggccggcggc ctccgtggac gagggcgagt tcgacctcga catccggctc
     1441 gtccctgctg gcgggccgtc ctccgggcgc cggcgcccgg ccagggccgc ctggtacgac
     1501 acggacgcgt gcggcgacac cgaggcgtgc ccgccggcga ccgagcaggg ctgcgacacc
     1561 gcgatccagt gcgacacggt cctgaactgc gacaccgacc tggactgcga gacggtggcc
     1621 gagtgcgaca ccgtcgtcga ctgcgccgac accgacctgg actgcgcgga cacgcagctg
     1681 gactgcgccg acacccgacg cgactgcgcc ggcgacacga tggtcgggtg cccgggcgac
     1741 accgaggtgg gatgcccggg cgacaccatc gtcgggtgcc cgggcgacac ctacgtcgac
     1801 tgcccggtcc cgaccgatct cgccaccaac tgctgcgagg agaccgacaa cacgtgcgcg
     1861 tcctgctaca ccgactgccg gatgtgcgtg ccctgagcag ggcgcatccg cctgaagcgc
     1921 gcgaggcctc gatgcctgac gcgcgcgagg ccgcctcagc ccgggcgcag ctcggggcag
     1981 aggaacgtcg cgtacaggcg cgcgtacacg cgggcggcgg tgagcaggtc ggcgactggc
     2041 acgcgctcgg cggtggagtg cgcgagggcg aggtcgcccg gcccgaggac gatcgcgggg
     2101 acgcccgcct ccaggttgag gatgcgggcg tcggtcgcgc cgccgaagcc gaccggctcg
     2161 gctgggagcc cggcgtcggc gagcgcggcg accgccgccc tgaccaccgc cgcatcgggg
     2221 tccacctcgc tggcggggct gaagccctgg ccgccgagct cggcccggaa gctggggtcc
     2281 gcggccgcct cgcggtcgag gatggcctgg aactcgtcga gcacctgctc ggccgtttcc
     2341 ccggggatgg tgcgccggtc gacctcggcc tcgcaccatg ccggcaccat gttgaccttg
     2401 tcgcccccgc ggatcgtgcc gacgttcacc gtcgggccgc ccaggagcgg atgggtgcga
     2461 tcccaggtca tgccgaggac cgcggggatc agcctggcca tggcggcgat cgcgctcacc
     2521 ccctcggccg ggcgcgcgcc gtgggcggcc cggccgagag tgcgcagcac gtaccaggac
     2581 gcgccgcgtt ccgctgtggc cagccgcatg ccggtcggct cggcgaccag ggcgccgtcg
     2641 gcctcgagca ggccggcggc gagcagcgcg cgggtgccgt gggtgccgac cgtctcctcg
     2701 tcggcgacga gctggaacac cagccggccg gtccaaggca ggccgctgtc gagcagcatc
     2761 ctcgcggcgc agagggcggc cgcgatcggc cccttcatgt cgagcgaccc gcggccgtgg
     2821 agcacgccgt cggcgaccac cgccccgtag ggcgggtgct cccactggtc ggccgggccg
     2881 gccggcacga cgtcgaggtg gccgttgacc gccagggtcg gccctggccg cagggagtcg
     2941 aacaggccca ccaggctgac gcggcccggc gccggctcca ccgcccggac ctggcagccg
     3001 aggtcgtcga gccaggggcg gacggccgcc gccgcggcac gctcgtcgcc cggcgggttc
     3061 tcgctcgccg ccctcaccag ggtgcgggcg cagaccagca gctcgtcggc gtccagcgtc
     3121 tcgtcgagcg tggccagctc gtcagacatc gccccccctt cgctctccgg gaccgcagtg
     3181 tacggggcgg aggaccggca aactaccgag gaacgcggtc gtgccggcac ccccgccagc
     3241 cggccggggt tggccgggcc cgcgcgggta gcatggcggg aggggctcgg gaccgaggag
     3301 gtggccatgg cgggcgacgg gtcggacgac cgcctggtcc ccgttcggca cgccgaggcg
     3361 tgcctccgaa acccccggga gctgctgcgc accgccaggg tgatcgccgt ggtcgggctg
     3421 tcgcccaacc cggaccgccc ctcgaaccag gtcgcctggt acctccacca ccaggggtac
     3481 cggctgtacg gggtccgtcc cgccgcagcc gggagcgaca tcttcggcgt gccggtggtg
     3541 gcctccctgg acgaggtgcc cgagccgatc gacgtcgtgg acgtgttccg ccgggcgccg
     3601 gacaccccgg acgtggcccg ggccgcggtg gcggtcggcg cccgggccct gtggctgcag
     3661 ctcggcatcg cgaacgccga gaccagggcc atcgccgagg cgggcggcct cgtctacgtc
     3721 gaggaccgct gcctcaaggt cgagcatctg cgcctgctcg gcgcccgcac cgggtagccg
     3781 cccgccggcc ggtcaggcga gaccgcggcg ggtgacggcc ggccggtgcc agccgaggat
     3841 cgcctcggtc atgcggacgg cgcggacggt cgcgcggacg tcgtggacgc ggacgacgtt
     3901 ggccccgccc atgatgctcg cgacggccgc cgcgaggctc ccctcgagcc gctcctcgag
     3961 cggcaggccg agggtctcac cgatgaagtc cttgttcgag agggcgacca tcagcgggta
     4021 gccgaggcgg cggagctcgc cgaggcggcg ggtcagctcg agcgagtgga aggtgttctt
     4081 gtggaagtcg tggcccgggt cgacgagcag gaggtcgggc ggcacgcccc ggtggcgggc
     4141 gaccgcggcc cggtcggcga ggaacgcggc cacctcggcg accacgtcgg cgtaggccgg
     4201 ccggtggggc cgggtgcgcg gcgggccgcc cgcgtgcatc acgaccagcg tcgcgccggc
     4261 ctcggccacg gcgtcggcca cccccgggtc gtgcagcccg gaggggtcgt tgatcacgtc
     4321 cgcg
//
LOCUS       DASPYY010000657         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2778211, whole genome shotgun sequence.
ACCESSION   DASPYY010000657 DASPYY010000000
VERSION     DASPYY010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2778211"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VEP73_10355"
     CDS             <1..220
                     /locus_tag="VEP73_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26870.1"
                     /translation="LKEFDGFFTLLVGTRTQFAVVRDAFACKPAVIAEGDGYVAMASE
                     YHALAGLPGIENADVFEPMPEEIHLWSR"
     gene            208..885
                     /locus_tag="VEP73_10360"
     CDS             208..885
                     /locus_tag="VEP73_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HYT26871.1"
                     /translation="MVTLSCAELSVTEINRALRDLPAGTAVRITEPRGSHNLAVGLRH
                     HLDITIDGNAGYYVAGLCDGPDVTVNGFVGWGVGENLMRGTVRVEGNASECAGASAHG
                     GTVVIVGDASSRAGISLKGGTVVIAGDVGHMSGFMAQAGTVLVGGDAGQALGDSLYEA
                     VIYVGGRVRSLGADARVEDLADEDLLKVKKLVELSGFDHVDPQNVTRIASAKQLYSFD
                     ALKSQRY"
     gene            887..2263
                     /locus_tag="VEP73_10365"
     CDS             887..2263
                     /locus_tag="VEP73_10365"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HYT26872.1"
                     /translation="MTDHTFDSDGRVPAGARPSHTFPPETIAQIQRMADEGRYEIRGW
                     GAKRRVPSFDDLVFLTASASRYPLEGYRERCDTRTVLGSRYASRPLELDIPITIAGMS
                     FGALSASAKEALGRAATAVGTSTTTGDGGMTPEERKASRLLVYQCLPSRYGFNPADLH
                     AADAIEVVVGQGAKPGGGGMLLGQKVSERVASMRTLPPGVDQRSASRHPDWTGPDDLR
                     IKIEELREATGWEKPVYVKLGATRVANDVKLAVAAGADVVVVDGMQGGTGATQDVFIE
                     HAGIPTLPAVRLAAEALREVGKQDEVQLVVSGGIRSGADVAKALALGADAVSIGVGAM
                     IALGCNRRGWFRDGVEHDAAADYAALGTAAGFCHHCHTGRCPVGVTTQDPDLERRLDP
                     ETGARWMTNYLKALTMELTTLARACGKSDVHNLEPEDLVALTVEAAAMARVPLAGTDW
                     IPGHDGAR"
     gene            2498..3547
                     /locus_tag="VEP73_10370"
     CDS             2498..3547
                     /locus_tag="VEP73_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HYT26873.1"
                     /translation="MSVFEQNATKTPQAEQTRPQFHRVVRGYDPLQVDRYVMQLITLV
                     EQQHRRAEEAEQSTRQLQSELETIRGEQPAPSFVHLGAEAARILEQAGTSAERLLGEA
                     KERAREIVQAAEEDGAERIKDAEERCGELELDARHTLEEAEAQRDEMLAEASDHADQT
                     RARVDGEARAVLAEARDASDLVWQELQQDRAVVEAETDRLRSFRENMLGHFEEIHVAL
                     RDLLEDARDEEELAAQAEARAAAARAAAADAVMARAAAEQARAAAEAEDDDADAEAPA
                     AVDAGADAEAEAPAGAGVDDGSEAATQQLEITQPVPGQAARPAGPATDRAGAAAQPAA
                     TTQQSTTEQGDCRKQ"
BASE COUNT          521 a         1305 c         1315 g          412 t
ORIGIN      
        1 cctcaaggag ttcgacggct tcttcaccct gctggtgggc acccgcacgc agttcgcggt
       61 cgtgcgggac gccttcgcct gcaagccggc cgtgatcgcg gagggcgacg gctacgtggc
      121 gatggcctcg gagtaccatg ccctggccgg gctccccggg atcgagaacg ccgacgtgtt
      181 cgagccgatg ccggaggaga tccacctgtg gtcacgctga gctgcgccga gctgtcggtg
      241 accgagatca accgcgccct gcgcgacctg ccggccggca cggccgtgcg catcaccgag
      301 ccgcggggca gccacaacct ggccgtgggc ctgcgccacc acctcgacat caccatcgac
      361 ggcaacgccg ggtactacgt cgcgggcctc tgcgacggcc ccgacgtcac cgtcaacggc
      421 ttcgtgggct ggggcgtcgg cgagaacctc atgcgcggca ccgtgcgcgt cgagggcaac
      481 gcgtccgagt gcgccggcgc gtccgcgcac ggcggcaccg tcgtgatcgt cggcgacgcc
      541 tcgtcccggg ccgggatctc gctcaagggc ggcaccgtcg tgatcgccgg ggacgtcggc
      601 cacatgagcg ggttcatggc ccaggccggc acagtcctcg tcggcgggga cgccggccag
      661 gcgctcggcg actccctcta cgaggcggtg atctacgtgg gtgggcgggt ccgctcgctc
      721 ggcgccgacg cgcgggtgga ggacctggcc gacgaggacc tgctgaaggt gaagaagctg
      781 gtcgagctgt ccggcttcga ccacgtcgac ccgcagaacg tcaccaggat cgcctcggcc
      841 aagcagctct acagcttcga cgccctcaag agccagcggt actgacatga ccgaccacac
      901 cttcgacagc gacggccggg tcccggccgg ggcccggccg agccacacct tcccacccga
      961 gaccatcgcg cagatccagc gcatggccga cgagggccgc tacgagatcc ggggctgggg
     1021 cgccaagcgg cgggtgccca gcttcgacga cctcgtcttc ctcaccgcct cagcgtcccg
     1081 ctacccgctc gagggctacc gggagcggtg cgacacgcgg accgtgctcg gctcccgcta
     1141 cgcgtcgagg ccgctcgagc tggacatccc gatcaccatc gccgggatga gcttcggggc
     1201 cctgtcggcc agcgccaagg aggcgctcgg ccgggcggcg accgccgtcg gcacgtcgac
     1261 caccaccggc gacggcggca tgacgcccga ggagcgcaag gcctcccggc tgctcgtcta
     1321 ccagtgcctg ccgtcccgct acggcttcaa cccagccgac ctgcacgccg ccgacgcgat
     1381 cgaggtcgtc gtcgggcagg gcgcgaagcc cgggggcggc gggatgctcc tcggccagaa
     1441 ggtgagcgag cgggtggcct cgatgcgcac cctgccgcca ggggtcgacc agcgctccgc
     1501 ctcgcggcac ccggactgga ccggcccgga cgacctgcgg atcaagatcg aggagctgcg
     1561 cgaggcgacc ggctgggaga agccggtcta cgtcaagctc ggcgcgacgc gggtcgcgaa
     1621 cgacgtcaag ctcgcggtcg ccgccggcgc cgacgtcgtc gtggtcgacg gcatgcaggg
     1681 cggcaccggt gccacccagg acgtgttcat cgagcacgcg ggcatcccga ccctgcccgc
     1741 cgtccgcctg gccgccgagg cgctgcgcga ggtcggcaag caggacgagg tgcagctggt
     1801 cgtgtcgggc gggatcaggt cgggcgcgga cgtcgcgaag gccctcgccc tcggcgccga
     1861 cgcggtctcg atcggggtcg gcgcgatgat cgcgctcggc tgcaaccgcc gcggctggtt
     1921 ccgcgacggg gtcgagcacg acgcggccgc cgactacgcc gcgctcggca cggcggccgg
     1981 gttctgccac cattgccaca ccggccgctg cccggtcggg gtgaccaccc aggacccgga
     2041 cctcgagcgg cggctcgacc cggagacggg cgcccgctgg atgaccaact acctgaaggc
     2101 gctgacgatg gagctgacca cgctggcgcg cgcctgcggg aagtccgacg tccacaacct
     2161 cgagcccgag gacctggtcg ccctgaccgt cgaggccgcc gccatggcca gggtcccgct
     2221 ggcgggcacc gactggatcc ccggccacga cggggcgcgg tagcgcgccc acggcgctcg
     2281 tgccgcccgg cgccaccgtg atcgtgtcgc gttcgtctca gttgatggat gcttgcgcag
     2341 aggacgagaa ttcccgccag cgtgctgacc gacgagcgtg gaacccgcgg gtgccgtgcg
     2401 agcccggcga cggttgggcc cgcccttgat ctctcgtctc tcctggatct ccagtctccg
     2461 gcccgccatc ccactaccag tgcagggagg ttccccattg agcgtcttcg agcagaatgc
     2521 caccaagacc ccgcaggccg agcagactcg gccgcagttc caccgggtcg tgcgcgggta
     2581 cgatccgctg caggtcgacc gctacgtgat gcagctcatc acgctcgtcg agcagcagca
     2641 ccggcgggcc gaggaggccg agcagtcgac acgccagctc cagagcgagc tcgagacgat
     2701 ccggggcgag cagcccgcgc cctccttcgt ccatctcggc gccgaggccg cgcggatcct
     2761 cgagcaggcc gggacgtcgg ccgagcgcct gctcggtgag gccaaggagc gcgcgcggga
     2821 gatcgtccag gcggccgagg aggacggcgc cgagcggatc aaggacgccg aggagcgctg
     2881 cggcgagctc gagctcgacg cgcgccacac cctcgaggag gccgaggcgc agcgggacga
     2941 gatgctggcg gaggcctcgg accacgccga ccagacccgc gcccgcgtgg acggcgaggc
     3001 ccgcgccgtg ctggccgagg cccgcgacgc gagcgacctc gtctggcagg agctgcagca
     3061 ggaccgcgcg gtcgtcgagg cggagaccga ccgcctgcgg tcgttccgcg agaacatgct
     3121 cgggcacttc gaggagatcc acgtcgccct tcgcgatctg ctcgaggacg cccgggacga
     3181 ggaggagctg gcggcgcagg cagaggcccg ggcggccgcg gcaagggccg ccgccgccga
     3241 cgccgtcatg gccagggccg ccgcggagca ggcgcgggcg gccgcggagg cggaggacga
     3301 cgacgcggac gcggaggccc cggccgccgt cgacgcgggc gccgacgcgg aggcggaggc
     3361 cccggccggt gccggggtcg acgacggctc ggaggccgcg acccagcagc tcgagatcac
     3421 ccagcccgtg cccgggcagg cggcccggcc cgccggcccg gcgacggacc gggcgggtgc
     3481 cgccgcccag ccggcggcca cgacgcagca gtcgacgacc gagcagggcg actgccgcaa
     3541 gcagtagcgg ccg
//
LOCUS       DASPYY010000658         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_107819, whole genome shotgun sequence.
ACCESSION   DASPYY010000658 DASPYY010000000
VERSION     DASPYY010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107819"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VEP73_10375"
     CDS             complement(<1..475)
                     /locus_tag="VEP73_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYT26874.1"
                     /translation="MGLLQGVALYTGAVLGTGVLALPSIAARQAGPASLLAWAGLVLL
                     SVPVASTFAALAGLYPDGGGVATFAARAFGPRWAAVTGWWFYCSVPVGVPVLGLFGGA
                     YVAAATGGGRATALAVGATLVAVAVAANWAGLRLSGRLQLALAGCLAALLVVAIAT"
     gene            550..1014
                     /locus_tag="VEP73_10380"
     CDS             550..1014
                     /locus_tag="VEP73_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYT26875.1"
                     /translation="MVLHDLDSIDRQLVEALVADGRATVTSLADLVGLSGPSVHERLR
                     KLEAAGAIRGYAAVVDPDAVGAGVAAFVALNLGPGLADKDRIDVALAREPSVLEVHEV
                     AGEDCYLLKLRVGSPRELSAAVARLRMLSPTVTTRTTVVLRTVLERPLLGSP"
     gene            1048..2373
                     /locus_tag="VEP73_10385"
     CDS             1048..2373
                     /locus_tag="VEP73_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HYT26876.1"
                     /translation="MPMWDQRAETMSADERAAVQSQRLGALVARLAGASTFYRRRLAD
                     AGVERGAALALDELTALPFTEKRDLWEHYPWGMLTIPRERVLRVHGSSGTGGRPTLVA
                     YSRTDLAIWAQVCARSLGCAGAGPGTVCHNAYGYGLFTGGLGMHAGAELMGCTLIPVS
                     GGQTQRQVTLMRDLEPEVLCCTPSYAARLGEALAEAGVPSGEISLRAGIFGAEPWSEA
                     MRAQLEQLLPLKALDIYGLSEIIGPGVACECIEAQAGLHVNEDHFLVEVVDPDTGAPL
                     PPRTRGELVFTTLTKEAMPLLRYRTGDVAALDPAPCVCGRTLVRMSKVLGRVDDMLVI
                     RGVNVYPSEVEAVLLAEPAVGPQYLLVVDRTETIPSLVVACELADPAGGGDQRDRMGE
                     SVAAALQRRLGLRTEVRVLPVGTLPRIEVGKAQRVVERTPGHDPLRGWG"
     gene            2457..>3084
                     /locus_tag="VEP73_10390"
     CDS             2457..>3084
                     /locus_tag="VEP73_10390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT26877.1"
                     /translation="MHPKFLTHDLNPAQREAVEATAGPVCILAGAGTGKTRVISRRVA
                     YAVATDAVHPGHVLVVTFTEKAAGEMRERLSGLGFPGVRAATFHAAALAQLRYFWPRV
                     SGTALPEVLDSKAPLLAPLQRSLPGGYRFTALKDLADEIEWAKARRLDPERYQAAAER
                     LGRTPPVPADLFAGLFRRYERAKERAGRIDFEDMLALTVQGLATDDGVA"
BASE COUNT          414 a         1152 c         1124 g          394 t
ORIGIN      
        1 aggtggcgat cgcgacgacg agcagggcgg cgaggcagcc ggccagggca agctgcaggc
       61 ggccggagag ccgcagcccg gcccagttgg ccgcgaccgc gaccgcgacg agcgttgcgc
      121 cgaccgccag ggcggtcgcc cgtccgccgc cggttgcggc ggccacgtac gccccgccga
      181 acaggccgag caccgggacg ccgaccggca ccgagcagta gaaccaccag ccggtcaccg
      241 ccgcccagcg cgggccgaac gcacgtgcgg caaaggtcgc gacgccgccg ccgtcagggt
      301 agaggccggc gagcgccgcg aacgtcgacg ccaccgggac cgagagcagc accagccctg
      361 cccaggccag cagcgaggcg ggtcccgcct gccgcgcggc gatcgagggg agcgccagca
      421 cgccggtccc gagcaccgcc ccggtgtaca gggcgacgcc ctgcagcaac cccatccgct
      481 gtccccgcgc cacggcagtt cctataatcc ttaggaaagc aatgcggttc cgcctagaaa
      541 gatgagacca tggtactaca tgaccttgat agcatcgatc gccagctggt cgaggcactg
      601 gtcgcggacg ggcgcgccac cgtcacctcg ctggcggacc tggtcgggct gtccgggcct
      661 tcggtccacg agcggctgcg caagctggag gcggccggcg cgatccgcgg ctatgccgcg
      721 gtggtcgacc cggacgcggt gggcgcaggc gtcgccgcgt tcgtcgccct gaacctcggc
      781 ccgggcttgg ccgacaagga ccggatcgac gtggcgcttg cgcgcgagcc gagcgtgctc
      841 gaggtgcacg aggtagccgg cgaggactgc tacctcctga agctgcgggt cgggtcgccg
      901 cgggagctgt ccgcggcggt cgcccgcctc cgcatgctca gtccgaccgt gacgacccgc
      961 actaccgtgg tgctccgcac cgtccttgag cgccccctcc tcggctcccc gtagcatctg
     1021 catgggcgac gaactcggga ggcgaggatg ccgatgtggg accagcgtgc ggagacgatg
     1081 tcggccgacg agcgagctgc cgtccagtcg cagcgactcg gcgcgctcgt cgcgcggctc
     1141 gcaggagcga gcacgttcta ccggcggcgc ctcgcggacg ccggcgtcga acgcggcgcg
     1201 gccctcgccc tcgacgagct gaccgcgctg ccgttcaccg agaagcgcga cctgtgggag
     1261 cactacccct ggggcatgct caccatcccg cgcgagcggg tgctccgcgt ccacggctcc
     1321 tccggcaccg gcgggcggcc caccctggtc gcctactccc gcacggacct cgcgatctgg
     1381 gcgcaggtgt gcgcccgctc gctcgggtgc gccggagccg ggcccggcac ggtctgccac
     1441 aacgcctacg ggtacggcct cttcaccggt gggctcggca tgcatgccgg ggcggagctc
     1501 atgggctgca cgctgatccc ggtctccggc ggccagaccc agcgccaggt caccctgatg
     1561 cgcgacctcg agcccgaggt gctgtgctgc acgccctcct atgcggcgcg gctgggtgag
     1621 gccctcgccg aggccggcgt accgtccggc gagatctcgc tccgggccgg catcttcggg
     1681 gccgagccct ggagcgaggc catgcgggct cagctcgagc agctgctgcc gctgaaggcg
     1741 ctcgacatct acgggctcag cgagatcatc gggcccgggg tcgcctgcga gtgcatcgag
     1801 gcccaggccg ggctccacgt caacgaggac catttcctcg tcgaggtcgt cgaccccgac
     1861 accggcgcgc cgctgccgcc gcggacccgt ggcgagctgg tcttcacgac cctcacgaag
     1921 gaggcgatgc cgctgctgcg ctaccggacc ggtgacgtcg ccgcgctcga ccccgcgccg
     1981 tgcgtgtgcg gccggacgct ggtgcgcatg tccaaggtgc tcgggcgcgt cgacgacatg
     2041 ctggtgatcc ggggcgtgaa cgtctacccg agcgaggtcg aggcggtcct cctggccgag
     2101 ccggcggtcg gcccccagta cctgctcgtg gtggaccgca ccgagaccat cccgagcctc
     2161 gtggtcgcct gcgagctggc cgacccggcg ggcggcggtg accagcggga tcggatgggc
     2221 gagtcggtcg ccgcggcgtt gcagcgccgc ctcgggctgc gcacggaggt ccgtgtcctg
     2281 cccgtcggca cgctgccccg gatcgaggtc ggcaaggcgc agcgcgtggt ggagcggaca
     2341 cccgggcatg atccgctccg agggtggggg tagacgatca ccagcgccgc gatcggcctc
     2401 gatccggggt ggacggccgt cggccgccgt cgggcgcctg tggcagactc gcccgcatgc
     2461 acccgaagtt cctcacgcac gacctgaacc ccgcccagcg cgaggcggtc gaggcgaccg
     2521 ccgggccggt ctgcatcctt gccggcgccg gcaccggcaa gaccagggtc atctcgcgcc
     2581 gggtggcgta cgcggtcgcg accgacgccg tgcacccggg ccacgtcctc gtcgtcacct
     2641 tcaccgagaa ggctgccggc gagatgcggg aacgactgtc cgggctcggc ttccccgggg
     2701 tccgggcggc tacgttccac gccgccgcgc tcgcgcagct ccggtacttc tggccacggg
     2761 tgtcgggcac ggcgctgccc gaggtgctcg actccaaggc cccgctgctc gcccccttgc
     2821 agcggtcgct gcccggcggc taccggttca cggccctcaa ggacctggcc gacgagatcg
     2881 agtgggccaa ggcgaggcgg ctcgacccgg agcgctacca ggccgccgcg gagcgcctcg
     2941 gccggacccc gcccgtgccc gccgacctct tcgccggcct gttccgccgc tacgagcgcg
     3001 ccaaggagcg cgccggccgg atcgacttcg aggacatgct ggcgctcacg gtccaggggc
     3061 tcgcgaccga cgacggggtc gccg
//
LOCUS       DASPYY010000659         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1235132, whole genome shotgun sequence.
ACCESSION   DASPYY010000659 DASPYY010000000
VERSION     DASPYY010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235132"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..500
                     /locus_tag="VEP73_10395"
     CDS             168..500
                     /locus_tag="VEP73_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HYT26878.1"
                     /translation="MRLVESAVASLPKEFRDRLANVEFVVEETFRGDEVPGGGLLLGL
                     YQGVPLPERGAGYSGVLPDRIVIFRRPIEARSRSDADLVELVREVVIHEVAHYFGIDD
                     DRLDELGW"
     gene            complement(593..>2160)
                     /locus_tag="VEP73_10400"
     CDS             complement(593..>2160)
                     /locus_tag="VEP73_10400"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HYT26879.1"
                     /translation="ERLADQPPPAGRPHPEDQPRPADSAIMPGRAARYAPPPARAGSG
                     GAARASRQGRPRPRLPAGSFKVTRAGLALAGVLVAAAGALVGITRVLPAAKAPAAPAA
                     EAPYAGRWVCPTLPRLPGAVTVTNVGAAPATLRTQVTPAPRPAAGAGRPQRAAGAGAG
                     TLAPGATRKITVNPSTAAGFVQVEAFGAPIAVASAQQPSCAPGPGDRWWLPGLTASST
                     TRSDLVLANPDSVDATVAITPHLTEGSIHPTPLQNIFIPAGTSVVKTIDVPDVQTLPF
                     TAEVVATSGRVVTGALVTTKTGRQTTQVLVPGQPTARSSWAFTGGLGQGVDSVDVLLA
                     NPNQGPLTVVAHATTDQGTFKAPGFDLPIADGAASVIRVPLNPGRTGAFSLLLESRDG
                     VKFVAALRLQGGGSTASFFETGGTSDDARWLLPEAPGSGRLVLANVSDEPVTAELSSL
                     GSPGRTGSSVTVPPGKVRLQAVPAGSRSVLVTASRPGMVAAPLGGGQVVPGSQVGGLP
                     VDGPVVPGPAAAP"
BASE COUNT          243 a          800 c          876 g          241 t
ORIGIN      
        1 ttggcgcgtc cccgcttgcc tgaagcggga ggcccgccgc ccagtcgccc cgcccgccgc
       61 acgaccagga ggccgccctg ccccgccagc gccacgcccg ccgcgccccg cggccgcggc
      121 tcgggctgcc ccaggtgcag ccgccgggca cgagcaccga cgccttcatg cggctcgtcg
      181 agtcggccgt cgcgtcgctg cccaaggagt tccgcgaccg gctcgccaac gtcgagttcg
      241 tcgtcgagga gaccttccgg ggggacgagg tcccaggagg cggcctgctg ctcggcctct
      301 accagggggt gccgctgccg gagcgcggcg ccgggtactc gggcgtcctg cccgaccgga
      361 tcgtgatctt ccggcgcccg atcgaggcgc gctcgcgctc ggacgccgac ctcgtcgagc
      421 tggtccgcga ggtcgtcatc cacgaggtgg cgcactactt cggcatcgac gacgaccggc
      481 tggacgagct cggctggtga cgggccccca cggccggggt gcgccgcgcc gggcacctgg
      541 ccgctacgcc ccaggggcac ccggctgcca gggcccggac gcctggctgc tgctacggcg
      601 ccgcggccgg gccggggacg accggcccgt cgaccgggag gccgccgacc tgggagccgg
      661 gcacgacctg gccgccgccg agcggggcgg cgaccatgcc gggccgggag gcggtgacga
      721 ggacgctccg ggagccggcg gggaccgcct gcaggcggac cttgcccggg ggcacggtga
      781 ccgagctgcc cgtccggccc ggcgacccca ggctggagag ctcggcggtg acgggctcgt
      841 cggacacgtt ggcgaggacc agacggccgc tgccgggcgc ctctggcagc agccacctcg
      901 cgtcgtcgct ggtcccgccg gtctcgaaga agctcgcggt ggacccgccc ccctggagcc
      961 ggagggcggc gacgaacttg accccgtccc gcgactcgag gagcagggag aacgcgcctg
     1021 tccggcccgg gttgagcggg acgcggatca cggaggcggc cccgtcggcg atcggcaggt
     1081 cgaaccctgg cgccttgaag gtgccctggt cggtggtggc gtgggcgacc accgtcagcg
     1141 ggccctggtt cgggttggcg agcagcacgt cgacgctgtc gacaccctgg ccgagaccgc
     1201 cggtgaacgc ccacgacgac cgggccgtcg gctggccggg cacgaggacc tgcgtggtct
     1261 gcctgccggt cttcgtggtg acgagcgcgc cggtcacgac gcggccggag gtggcgacca
     1321 cctcggcggt gaacggcagg gtctgcacgt cgggcacgtc gatcgtcttg accaccgagg
     1381 tgcccgccgg aatgaagatg ttctgcagcg gcgtcgggtg gatcgagccc tcggtcaggt
     1441 gaggggtgat ggcgacggtc gcgtcgaccg agtcggggtt ggccagcacg aggtcgctcc
     1501 tggtcgtgct gctcgcggtc aggccgggca gccaccagcg gtcgcccggc cccggcgcgc
     1561 acgacggctg ctgggcggag gcgaccgcga tgggcgcccc gaacgcctcg acctggacga
     1621 agccagcagc ggtgctcggg ttcaccgtga tcttcctggt cgcgcccggg gcgagcgtgc
     1681 ccgccccggc gcccgcggcg cgctgcggcc tccccgcccc ggcggccgga cgcggcgccg
     1741 gagtcacctg ggtacgcagg gtcgccgggg ccgcgccgac gttggtgacg gtgacggccc
     1801 cgggaaggcg cggcagcgtc gggcagaccc agcggcccgc gtagggcgcc tcggcggccg
     1861 gggcggccgg ggccttggcg gccgggagga cccgcgtgat gccgacgagc gcgccggccg
     1921 cggcgacgag gaccccggcg agcgccaggc cggcccgggt gaccttgaag gagccggcag
     1981 gcagccgcgg cctggggcgg ccctgccggc tcgcgcgggc cgcgccaccg gacccggcgc
     2041 gggccggcgg gggcgcgtag cgcgccgccc ggcccggcat gatcgcggag tccgccgggc
     2101 ggggctggtc ctcgggatgg ggccggcccg ccggaggagg ctggtccgcc aggcgctcct
//
LOCUS       DASPYY010000660         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2778544, whole genome shotgun sequence.
ACCESSION   DASPYY010000660 DASPYY010000000
VERSION     DASPYY010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2778544"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VEP73_10405"
     CDS             complement(<1..430)
                     /locus_tag="VEP73_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26880.1"
                     /translation="MRSAQRMPGPASTARLGSNGDQSGPPAPGPAPRRRRGRVRRIAG
                     LAAAGWVLLFAGILIGTQVKGDGAATPARSERSAVDGARCAQAIARANESLAMAVRVE
                     RSLADRTRLRQLETGGELTPARARELDSVAAQRGAMASSRF"
     gene            complement(526..1377)
                     /gene="trpA"
                     /locus_tag="VEP73_10410"
     CDS             complement(526..1377)
                     /gene="trpA"
                     /locus_tag="VEP73_10410"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYT26881.1"
                     /translation="MPDAPPAAPPAERLEAHLEALRAGGRAALVAYGVAGYPDLPGSL
                     AAFRAMAEAGADVLEVGPPYSDPLIDGPVIQRAVQAALERGTRLDDVFAMVSELTASV
                     AVPVVLLVYYNLVAHRGHDRFARELAEAGACGAVVPDLPPEEAGGWVAAAARAGVAPV
                     FLAAPTSSDDRLRAVAAAGRGFVYAQASLGVTGLRVSLAAGIDDLVARVRTHTGLPVC
                     AGIGVSDAGQAAAVARFADGVIVGTALVRRLGEQGAGGLGALTGELAAAVHGARGAAP
                     PPGPGQP"
     gene            complement(1370..>1658)
                     /locus_tag="VEP73_10415"
     CDS             complement(1370..>1658)
                     /locus_tag="VEP73_10415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699561.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYT26882.1"
                     /translation="GLDYPGVGPEHAWWRDTGRVAYRQAGDALALDGFRLLARTEGLL
                     PALEPAHAVGWLAEAARGGEVAPGTVVVLNLSGRGDKDVDTVAGLLGGVDA"
BASE COUNT          176 a          638 c          684 g          160 t
ORIGIN      
        1 cgaacctgga ggaggccatc gcgccccgct gggccgcgac cgagtcgagc tcgcgggcgc
       61 gggccggggt gagctccccg ccggtctcga gctgccgcag gcgcgtgcgg tcggcgaggg
      121 agcgctcgac cctgacggcc atggccaggc tctcgttggc ccgggcgatc gcctgggcgc
      181 agcgcgcgcc gtcgaccgcg ctacgctccg accgcgccgg ggtcgccgca ccgtcgccct
      241 tgacctgcgt gccgatgagt atgccggcga acagcaggac ccagcccgcg gcggcgaggc
      301 cggcgatgcg ccggacccgg cccctccgcc ggcgcggcgc gggcccggga gctggcgggc
      361 cgctctggtc gccgttgctc cccagcctgg cggtggacgc gggaccgggc atgcgctggg
      421 cacttcgcat ggcgtccgtc tccttgtggt tcgggcgtgg ttcgggcgtg gttcgggcat
      481 ggggcgggcc ggcggctgcg cgacggccgg gcccgggcgg ggaggctacg gctgccccgg
      541 accgggcggc ggcgcggcgc cgcgggcgcc gtgcacggcg gcggcgagct cgccggtgag
      601 cgcgcccagc ccgccggcgc cctgctcgcc gagccggcgc accagcgcgg tccccacgat
      661 cacgccgtcg gcgaagcggg ccacggccgc ggcctgcccg gcgtcggaga cgccgatgcc
      721 ggcgcagacg ggcagcccgg tgtgggtgcg gacgcgggcg acgaggtcgt cgatgcctgc
      781 cgccagcgac acgcgcaggc cggtcacgcc gagcgacgcc tgcgcgtaga cgaacccgcg
      841 gccggcggcg gcgacggccc ggagccggtc gtcgctcgag gtcggcgcgg cgaggaagac
      901 gggtgccacg ccggcccgcg ccgccgccgc gacccacccc cccgcctcct cgggcgggag
      961 gtcgggcacg accgcgccgc acgcccccgc ctcggccagt tcgcgggcga agcggtcgtg
     1021 gccgcggtgc gcgaccaggt tgtagtagac gagcaggacg accgggacgg cgaccgaggc
     1081 ggtcagctcg ctcaccatcg cgaacacgtc gtcgaggcgg gtgccgcgct cgagggcggc
     1141 ctgcaccgcc cgctggatga cgggcccgtc gatcagcggg tcggagtagg gcggcccgac
     1201 ctcgagcacg tcggcgcccg cctcggccat ggcccggaag gcggcgagcg acccgggcag
     1261 gtcggggtag cctgcgacgc cgtaggccac gagggccgcc cgcccgccgg cgcgcagggc
     1321 ctcgaggtgc gcctccagcc gctcggccgg cggggcggcc ggcggggcgt caggcatcga
     1381 cgcccccgag caggccggcg accgtgtcga cgtccttgtc gccccggccg gagaggttga
     1441 gcaccacgac cgtgcccggc gccacctcgc cgccgcgggc cgcctcggcg agccagccga
     1501 cggcgtgcgc gggctcgagg gcgggcagca gcccctcggt ccgggcgagc agccggaacc
     1561 cgtcgagggc gagcgcgtcg cccgcctgcc ggtaggcgac ccggccggtg tcgcgccacc
     1621 aggcgtgctc cgggccgacg cccgggtagt cgaggccg
//
LOCUS       DASPYY010000661         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_250205, whole genome shotgun sequence.
ACCESSION   DASPYY010000661 DASPYY010000000
VERSION     DASPYY010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250205"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VEP73_10420"
     CDS             <1..138
                     /locus_tag="VEP73_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT26883.1"
                     /translation="ASSVGQLEDNVAALQGLQFGDDELAEIDRYASEGGINLWADSSA
                     V"
     gene            complement(157..996)
                     /locus_tag="VEP73_10425"
     CDS             complement(157..996)
                     /locus_tag="VEP73_10425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT26884.1"
                     /translation="MSSVLIYGGAALSHVDQDVADRSVQLALDAGINHFDVAASYGDA
                     ELRLGPWMPRIRDRIFLATKTGRRDSASAWEELNRSLERLRTEHVDLIQLHAVGDLAE
                     LDLVTGRGGSLEAAVRARQEGLAGAIGITGHGHEAPATHREALRRFPFDTVLTPLNYV
                     LSQDPAYLSDYEALVADVRAQDAGLMVIKAVARRNWPATAGQQYATWYEPFDDQERVT
                     AAVVWSLSHPEVTGIATPGDVRLLPLVVGAEKRLGDTSPVEAEEVLARAAGYSSPFID
                     MPF"
     gene            1268..1969
                     /locus_tag="VEP73_10430"
     CDS             1268..1969
                     /locus_tag="VEP73_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HYT26885.1"
                     /translation="MTTTEGPGREEEAPLRRPPQLAMLVEGGDRKGLLHDMTAAILRH
                     GGDILSVEIVERGARSAVYWELDAVEDPGSLERSFLAIDVVASVRRLPSMFQVYGKRI
                     IVVGAGAQVGQVALGAIGEADRHNIRGEKISVDTIPVVGEEYLANAARAVARLPRAVA
                     LVLAGSLMGGEVTEAVDEVRARGILVVSLNMAGSVPEHADLVVTDPVQAGVMAVMAVA
                     STARFDIARVRGRRY"
     gene            2123..2404
                     /locus_tag="VEP73_10435"
     CDS             2123..2404
                     /locus_tag="VEP73_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HYT26886.1"
                     /translation="MSAYAPQPNLDDPHLAMVRPVVPRTPEGCEECLQIGSPWVHLRL
                     CLTCGHVGCCDSSPLRHARGHADAVRHPIVQSFQPGEDWRWCYLDETYV"
     gene            complement(2549..3052)
                     /locus_tag="VEP73_10440"
     CDS             complement(2549..3052)
                     /locus_tag="VEP73_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HYT26887.1"
                     /translation="MRPSGADQEHRDRSWIAALLGYGEFVLDAAVAVALSVGGVILLS
                     VVVYDFARGLGHGRVIDQVLGLLSGLLLVFIFTELIHTIRVVIARRAVEVEPFLIVGI
                     VAAVRRLIVISAEAENLLGTARFRDLMLEIGVLAGTVVLLGTTVLLLRASHRRHGEPP
                     TGPAGDH"
     gene            3191..>3788
                     /locus_tag="VEP73_10445"
     CDS             3191..>3788
                     /locus_tag="VEP73_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26888.1"
                     /translation="MTRRREDSARRASTYKLLLGLPGVGLQALCGMLAQLTQGAAAVG
                     MILVVHESTGSLAIAGGVTAGFSAGVCVARPAQGRLIDRRGAPPVLTACAVLHVGALI
                     GLVAAAGSAAPAWALIQLGCLAGLGLPPVSTSMRVAWGRRMPGEGRTAAFSLVYLTQT
                     MAILVGPLLLGALVALASASLALAAVAGVAGGGTLVFAR"
BASE COUNT          510 a         1377 c         1357 g          544 t
ORIGIN      
        1 gcgagcagcg tcggccagct cgaggacaac gtcgccgccc tgcaaggtct ccagttcggc
       61 gacgacgagc tcgccgagat cgaccggtac gcgagcgagg gcggcatcaa cctgtgggcc
      121 gactccagcg ccgtctgacg ctcgaggcga ggagggtcag aacggcatgt cgatgaacgg
      181 cgagctatag cccgcggccc gggcgaggac ctcctccgcc tcgacggggc tggtgtcgcc
      241 gaggcgcttc tccgccccga cgacgagggg cagcagccgt acgtcgcccg gcgtcgcgat
      301 gccggtgacc tccgggtgtg acagcgacca gacgaccgcg gcggtgaccc gctcctggtc
      361 gtcgaagggc tcgtaccagg tggcgtactg ctggccggcc gtggccggcc agttgcggcg
      421 cgcgaccgcc ttgatgacca tgagccccgc gtcctgcgcc cgcacgtcgg cgacgagcgc
      481 ctcgtagtcg gacaggtacg ccggatcctg gctcagcacg tagttgagcg gcgtgagcac
      541 cgtgtcgaac gggaaccgcc gcagcgcctc gcggtgggtc gccggcgcct cgtgcccgtg
      601 cccggtgatc ccgatcgcgc cggccaggcc ctcctgcctg gcccggaccg ccgcctcgag
      661 cgagcctcca cgcccggtca ccaggtcgag ctccgcgagg tcgccgaccg cgtgcagctg
      721 gatgaggtcg acgtgctcgg tccgcagccg ctccaggctc cggttgagct cctcccaggc
      781 gctggcgctg tcccgccggc ccgtcttggt cgccaggaag atccggtcgc ggatgcgggg
      841 catccatggt ccgagccgca gctcggcgtc gccgtagctg gccgcgacgt cgaagtggtt
      901 gatccccgcg tcgagcgcca gctggaccga gcggtcggcg acgtcctggt cgacgtggct
      961 gagcgcggcc ccgccataga tcaggacaga gctcatgtga ccgaggcgac ccagtcttcg
     1021 ttgctccacg acacccgctc ctcttctcgc cgtcccgccg tctcgacctc cgcctgaggc
     1081 gcgcgaggcg cgccgcaccc acggtagcgc ccgcggcctg ctgctactac agcgggcgct
     1141 cgagcaggtc ctggaagctg gccgggacgg ccgctgcctt ctcgtccacg atcgacctcc
     1201 ggtgtcgccg gttccatcgc cccattctga ccctcacgac gccgagcgac cctggtaggt
     1261 tctggccgtg acgacgacgg agggaccggg ccgggaggag gaggcgccgc tccggcgccc
     1321 gccccagctc gccatgctgg tggagggcgg cgaccgcaag gggctgctcc acgacatgac
     1381 cgcggcgatc ctccggcacg gcggcgacat cctctcggtc gagatcgtcg agcggggcgc
     1441 ccgcagcgcg gtgtactggg agctggacgc cgtggaggat cccgggagcc tggagcggtc
     1501 gttcctggcc atcgacgtcg tcgcgtcggt ccgccggctg ccgagcatgt tccaggtcta
     1561 cggcaagcgg atcatcgtgg tcggcgcggg cgcccaggtc ggccaggtcg ccctcggcgc
     1621 gatcggcgag gccgaccggc acaacatccg tggcgagaag atcagcgtcg acacgatccc
     1681 ggtcgtgggc gaggagtacc tggcgaacgc ggcgcgggcg gtggccaggc tccccagggc
     1741 ggtggccctg gtgctggccg ggtcgctgat gggcggggaa gtgaccgagg ccgtggacga
     1801 ggtgcgggcc cgcggcatcc tggtcgtctc gctgaacatg gccggctcgg tccccgagca
     1861 cgccgacctc gtggtcaccg accccgtgca ggccggggtc atggcggtga tggccgtggc
     1921 gagcaccgcc cgcttcgaca tcgcgcgggt ccgcggccgc cgctactgac ctgtgacctg
     1981 accggcctct gttccagcag tcggcgccgc agtgcttacc gacccgcaag ggtgcaacct
     2041 agagggagcg agaactcgag ccgccaccaa ggtctgtcgt caaggtctgt cgtccacgag
     2101 caaggccgcg aggtgaagcc agatgagcgc ctacgccccc caacccaacc tcgacgatcc
     2161 ccacctggcc atggttcgcc cggtcgtgcc gcggaccccg gagggctgcg aggagtgcct
     2221 gcagatcggc tctccgtggg tgcatctccg gctgtgcctg acctgcgggc acgtcggctg
     2281 ctgcgactcc tccccgctcc gtcacgcccg cgggcacgcc gatgccgtgc ggcacccgat
     2341 cgtccagtcg ttccagcctg gcgaggactg gcgctggtgc tacctcgacg agacctacgt
     2401 ctgaggccta cgcggtcttc accccgacca gctcctccgg cggggccctg acgggggctc
     2461 gcgcccgtca gttcagtccg tcgtgcagga tggccacgat cacccgcccg gcgaccccgc
     2521 cgatttccga gccgacgccg gccccgcctc agtggtcacc cgcggggccg gtgggcggct
     2581 cgccgtgtct tctgtgactc gcgcggagca ggagcacggt ggtgccgagg agcaccaccg
     2641 tgccggcgag gacgccgatc tcgagcatca ggtcccggaa ccgggcggtc ccgagcaggt
     2701 tctcggcctc ggcgctgatc acgatcaggc gtcggaccgc cgccacgatc ccgacgatca
     2761 ggaagggttc gacctcgacc gcgcggcggg cgatgacgac gcggatcgtg tggatcagct
     2821 cggtgaagat gaacacgagc agcaggccgc tcagcagccc gagcacctgg tcgatgaccc
     2881 gcccgtgacc aaggccgcgc gcgaagtcgt agacgacgac actcaacagg atgaccccgc
     2941 cgacgctgag cgccaccgcc accgccgcgt cgaggacgaa ctcgccgtag ccgagcaggg
     3001 ccgcgatcca ggaccggtcg cggtgctcct ggtcagcacc tgagggcctc acgacccgga
     3061 tcgttcacga acgaggtgag cttgtccacc ggcacggagg gccgtcgtgg ctcgtcccga
     3121 tcacgagacc ccggtacggg tcgacgagca gaatggaggc catgagcgtc cctgcttcca
     3181 gtccggcccc gtgacgagac ggcgcgagga cagcgccaga cgggcgagca cctacaagct
     3241 gctcctcggc ctgccagggg tgggcttgca ggcgctctgc gggatgctcg cgcagctcac
     3301 gcagggtgcc gcggcagtcg gcatgatcct ggtggttcac gagagcacgg gttcgctggc
     3361 gatcgccggc ggggtgacgg cgggtttctc tgccggcgtc tgtgtcgctc gtccggccca
     3421 gggccggctg atcgaccgcc gcggggctcc ccccgtgctc acggcctgcg cggtcctgca
     3481 cgtcggcgcg ctcatcggac tcgtcgcggc ggccggctcc gccgccccgg cgtgggcgct
     3541 gatccagcta gggtgcctcg ccggccttgg actgccaccg gtgtcgacga gcatgcgcgt
     3601 cgcctggggg cggcgcatgc cgggcgaggg ccgcaccgcc gccttcagcc tggtctacct
     3661 cacccagacg atggccatcc tggtcgggcc gctgctcctc ggcgcgctcg tcgcgctggc
     3721 ctcggcgtcc ctggccctcg ccgcggtcgc gggggtcgcg ggcggcggca cgctcgtctt
     3781 cgcccgcc
//
LOCUS       DASPYY010000662         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1093242, whole genome shotgun sequence.
ACCESSION   DASPYY010000662 DASPYY010000000
VERSION     DASPYY010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093242"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VEP73_10450"
     CDS             <1..1576
                     /locus_tag="VEP73_10450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYT26889.1"
                     /translation="VAGPLPTAARGAVRVLVQKLAARDLGPERTVARLGGRVTRELPI
                     VGGFAATLPAAAVERLARASGVRAVSRDGRMRVQGSLAASSPSTPRSVYPQVVRADAA
                     WKAGATGSGVTVAIVDTGVSDVPDLHGRLVQVTDDLTGQVSSCVNLSGEPSCDDSYGH
                     GTFVAGIVAGNGASSGGKWKGAAPGARILSLKIAGRDGAADVSNVLAAIQWVVSFKDR
                     YGIGVLNLSLGTDSTQSASVDPLNYAVERAWAAGIAVVVSASNRGPDPRTISKPGDDP
                     YVITVGAIDDNGTAPLGDDRLPDFSSRGPTAADGLAKPDLVAPGAHVVSLRAPGSAID
                     AAFTNYVDGSYRKGSGTSMAAGVVSGVAALMLEANPGMTPDRLKYALAATARATVSTD
                     PMAVGAGLVDAYGASFGAPAGVANQGLARSSGLGSLDLSRGTVRTQTSDPLLGVVLGV
                     VLGGLLTAQLALWDPLGWTTGSWTPPTWYSTGWYTSRWYRITWAGNDWGGYNWRGASG
                     WYGSAWYGSAWYGGWE"
     gene            1581..>2969
                     /locus_tag="VEP73_10455"
     CDS             1581..>2969
                     /locus_tag="VEP73_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYT26890.1"
                     /translation="MADDDGHGSGGKAQRDAGAFPPSIPVCTMALALAGAVAGGAATP
                     PGPPRGVSWVAVAAFLATLTLAGSLLVEYRYRGGRVRYGEEGNSFDLFEAVLLPLVFA
                     LPGLGAVVVAGAAKALSERLLRVPLVVACYNVAQWMCATAVGSLVFVDLRPARSSSVR
                     DVPALLAAMLAVMLTNYLAFVVALSLSRRQPVHRVLAATAPGVVPFAVASTVNVAFGL
                     LFLATYAWEPAAAPLFVVPLAVLHWAGRAYAAVRADRARLQGMQRAMHALGVPVDPRE
                     AIPRFLLEVRECFEAEAAELIVVAGRARTVHRSGAGTPAYVSYQEPMGAGTLATALLE
                     QEEAVRVAPGSGDTGLLDLLLADGWRNCLAAPVRTGGQTLGALVVYDRTGMEGFEDGE
                     LAVLETLAGEVAAALQKGDLLEAIMEERTKLAEIFSNTSDGIATIDPDGTVASWNPGF
                     ERITGYRAEDVVG"
BASE COUNT          321 a         1150 c         1091 g          407 t
ORIGIN      
        1 agtcgccggc ccgctgccca ccgcggcgcg cggcgccgtc cgcgtcctcg tccagaagct
       61 ggccgcccgc gacctcggcc cagagcgcac ggtggcccgg ctcggcgggc gcgtcacccg
      121 cgagctgccg atcgtcggcg ggttcgcggc caccctgccc gccgccgccg tggagcgcct
      181 cgcccgtgcg agcggcgtcc gggccgtctc gcgcgacggc cgcatgcggg tccagggcag
      241 cctcgcggcc tcgtccccgt ccaccccgcg gtcggtctac ccgcaggtgg tgcgggccga
      301 cgccgcgtgg aaggctggcg cgaccggctc gggcgtgacc gtcgcgatcg tcgacacggg
      361 cgtctccgac gtgcccgacc tgcacggccg gctggtgcag gtgaccgacg acctcaccgg
      421 ccaggtgtcc tcctgcgtga acctgtccgg cgagccgagc tgcgacgact cctacgggca
      481 cggcaccttc gtcgccggca tcgtcgccgg gaacggcgcc tcgtccggcg ggaagtggaa
      541 gggcgccgcc cctggcgcgc ggatcctgtc gctgaagatc gccggccgcg acggcgcggc
      601 cgacgtctcc aacgtgctcg ccgccatcca gtgggtggtg tcgttcaagg accggtacgg
      661 catcggggtc ctcaacctgt ccctggggac cgactcgacc cagtcggcat cggtcgaccc
      721 gctgaactac gccgtcgagc gcgcctgggc ggccgggatc gccgtggtcg tgtcggcgag
      781 caaccgcggt cccgacccgc gcaccatctc caagccgggc gacgacccct acgtgatcac
      841 cgtcggcgcg atcgacgaca acggcaccgc gccactcggc gacgaccggc tgcccgactt
      901 ctcctcccgc ggccccacgg cggccgacgg gctggccaag ccggacctcg tcgcccccgg
      961 cgcgcacgtc gtgtccctgc gcgccccggg cagcgcgatc gacgccgcgt tcaccaacta
     1021 cgtggacggc tcctaccgca agggcagcgg cacctcgatg gccgcgggcg tggtgtccgg
     1081 cgtcgccgcc ctcatgctcg aggccaatcc gggcatgacc ccggaccggc tcaagtacgc
     1141 gctcgcggcg accgcgcggg cgaccgtgtc gaccgacccg atggccgtcg gcgccggcct
     1201 ggtggacgcg tacggcgcct ccttcggtgc gccggccggc gtcgcgaacc aggggctcgc
     1261 gcgctcgagc gggctcggca gcctcgacct ctcccgcggc acggtgcgga cgcagacaag
     1321 cgacccgctc ctcggcgtcg tgctcggcgt cgtgctcggc ggcctgctca ccgcgcagct
     1381 tgcgctgtgg gacccgctcg gctggacgac cggctcgtgg acgcctccga cctggtactc
     1441 gaccggctgg tacacctcgc gctggtaccg gatcacctgg gcgggcaacg actggggcgg
     1501 ctacaactgg cgcggcgcgt ccggctggta cgggtccgcg tggtacggct cggcgtggta
     1561 cggcggctgg gagtaggtgg atggccgacg acgacggtca cggatccggg gggaaggcgc
     1621 agcgggacgc cggcgccttc cccccctcga tccccgtgtg caccatggcg ctggcgctcg
     1681 ccggcgccgt ggcgggcggc gcggcgaccc ccccggggcc gccgcgcggc gtctcgtggg
     1741 tcgccgtcgc ggccttcctc gccacgctga cgctggccgg gtcgctgctg gtggagtacc
     1801 gctaccgcgg cgggcgggtc cgctacggcg aggagggcaa ctcgttcgac ctcttcgagg
     1861 ccgtcctgct gccgctcgtc ttcgccctgc ccgggctcgg cgccgtggtg gtcgcgggcg
     1921 ccgccaaggc gctctcggag cggctgctcc gggtcccgct cgtcgtcgcc tgctacaacg
     1981 tcgcccagtg gatgtgcgcg accgcggtcg gcagcctcgt gttcgtcgac ctgcgccccg
     2041 cccgctcctc gtccgtgcgt gacgtgccgg cgctgctggc cgccatgctc gccgtcatgc
     2101 tcaccaacta cctggcgttc gtcgtcgcgc tctcgctcag ccgccggcag cccgtccacc
     2161 gggtgctcgc cgcgaccgcg cccggcgtcg tccccttcgc ggtcgcctcg acggtcaacg
     2221 tcgccttcgg cctcctgttc ctcgcgacct acgcgtggga gccggcggcc gccccgctgt
     2281 tcgtggtccc gctcgcggtc ctgcactggg cgggccgggc gtacgcggcc gtccgggcgg
     2341 accgcgcccg cctgcagggg atgcagcgcg ccatgcacgc cctcggggtc cccgtggacc
     2401 cgcgggaggc gatcccgcgg ttcctgctcg aggtgcgcga gtgcttcgag gcggaggccg
     2461 ccgagctgat cgtcgtcgcc gggcgggcgc gcacggtcca ccggtcgggc gccggcaccc
     2521 ccgcctacgt gagctaccag gagccgatgg gcgccgggac gctcgccacc gcgctgctcg
     2581 agcaggagga ggcggtgcgg gtcgcgcccg gctcgggcga cacgggcctg ctcgacctgc
     2641 tgctcgccga tggctggcgc aactgcctgg ccgccccggt gcgcaccggc gggcagaccc
     2701 tcggcgcgct ggtggtctac gaccgcaccg gcatggaggg cttcgaggac ggcgagctgg
     2761 ccgtgctgga gacgctcgcc ggcgaggtcg cggccgcgct gcaaaagggc gacctgctgg
     2821 aggcgatcat ggaggagcgg accaagctcg ccgagatctt ctccaacacc tccgacggga
     2881 tcgcgaccat cgaccccgac ggcacggtcg cgagctggaa ccccgggttc gagcggatca
     2941 ccgggtaccg cgccgaggac gtcgtcggc
//
LOCUS       DASPYY010000663         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_250310, whole genome shotgun sequence.
ACCESSION   DASPYY010000663 DASPYY010000000
VERSION     DASPYY010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250310"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEP73_10460"
     CDS             <1..213
                     /locus_tag="VEP73_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYT26891.1"
                     /translation="AVASGAEPDDTSVQDIMTGDVVTVGPDWDLVEAAAEMARRRIRH
                     LVVYEGGQLLGILSVRDVLPALLPGR"
     gene            complement(236..853)
                     /locus_tag="VEP73_10465"
     CDS             complement(236..853)
                     /locus_tag="VEP73_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26892.1"
                     /translation="MRASSHLSRSRSHGGNAATGLLFVGVVMVVLFAAGIGAVALTGN
                     RQPAPAIGPSTAQVNAKVAADRVGGAAAARRAAAARRLTEIVSLRDAALARRDAALLD
                     RIYTPTSANLRMDRASIALLRQRHVHWVGLATSVQVVDAHPAGARQWTVLATFACAPA
                     RLVTESGRQVEAARARSALLRFTLVLPPGGGKLLLSRIAPARSGD"
     gene            complement(974..>2385)
                     /locus_tag="VEP73_10470"
     CDS             complement(974..>2385)
                     /locus_tag="VEP73_10470"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYT26893.1"
                     /translation="ARAARLYQRVFDEYGDDSVAQLGGAHVACEQASNLLTKALEWGR
                     LAAYLEQSTRYVSYADRPGGRWRYHLDPEVAADAELGPEYRRVLDGLFETYAELVPVL
                     TEHLARVVPHEPGTSEPAWRRAVRARALDALRGLLPAATTSNLGIYASGQAYEALLVR
                     LAAHPLPEARSYGELLLAELRMVIPAFLTRVDRDDRGVAWSRYLARTAEETAEVVGAV
                     LGPGTGAEEGPSVRLSEFDPAGEDKVLTAIAYPHLREGEASVAAQVARLGAADRAGLF
                     AAYVGERQNRRHKPGRAFERSVYTFDVVADYGAFRDLQRHRLCTIVWQPLTPELGYEV
                     PAEVEAAGRGDRFRAAAAASAGLYHALEPRFPAQASYAVSLAHRVRFAITLNARELLH
                     LAELRSAPQGHPAYRVVAQAMHRLVAERAGHRALAEAMRFVVHDEADLGRLDAERRLE
                     ARRPAERVAIPSRIARRTE"
BASE COUNT          303 a          849 c          948 g          285 t
ORIGIN      
        1 gccgtcgcca gcggcgcgga gccggacgac acttcggtgc aggacatcat gaccggcgac
       61 gtggtgaccg tggggccgga ctgggacctg gtcgaggcgg ccgccgagat ggcgcgcagg
      121 cgcatccggc atctggtcgt ctacgagggc ggccagctcc tcggcatcct gtccgtccgc
      181 gacgtgctgc cagccctgct gcccggccgc tgagccgccg ccccgcccgg cgcggtcagt
      241 ccccggaccg cgccggcgcg atgcgggaga gcagcagctt gccgccgccc ggcggcagga
      301 ccagcgtgaa gcgcaggagc gccgaccggg cccgggcggc ctcgacctgc cggccggact
      361 cggtcacgag cctcgccggt gcgcaggcga aggtggccag gacggtccac tgccgcgccc
      421 cggccgggtg agcgtcgacg acctggaccg aggtggcgag cccgacccag tggacgtgcc
      481 gctgccgcag cagggcgatg gaggcccggt ccatccgcag gttggcgctc gtcggcgtgt
      541 agatccggtc gagcagcgcc gcgtcgcggc gggccagcgc cgcgtcccgc agcgagacga
      601 tctcggtcag ccggcgcgcg gccgccgccc ggcgggcggc ggccgcgccg ccgacccggt
      661 cggcggcgac cttggcgttg acctgggcgg tgctcggccc gatggcgggc gccggctgac
      721 ggttgccggt gagggcgacc gcgccgatgc cggcggcgaa gaggaccacc atgacgacgc
      781 cgacgaacag caggccggtg gcggcgttgc cgccgtggga ccgggaccgg gagaggtggg
      841 agctggctcg catagacgat ggcaaccctc ggggataggc cgccgagatg ctagggcgct
      901 gggaaatgcg ttacaaatcg tcgatcagga tgatcctgtc atctgtgcgc ctggtctgtc
      961 ggcttcgaca ggatcattct gttcgtcggg cgattcgtga gggaatcgca acgcgctccg
     1021 ccggtcgcct cgcctcgagg cgccgctcgg cgtccaggcg cccgaggtcg gcttcgtcgt
     1081 gcacgacgaa gcgcatcgcc tcggccagcg cgcggtggcc ggcccgctcg gccacgagcc
     1141 ggtgcatcgc ctgcgccacc acccggtagg cggggtggcc ctggggtgcc gagcgcagct
     1201 cggccaggtg gagcagctcg cgggcgttca gggtgatcgc gaagcggacc cggtgggcga
     1261 ggctgaccgc gtaggaggcc tgggccggga agcgcggctc gagcgcgtga tagagcccgg
     1321 ccgaggctgc cgccgcggcc cggaaccggt ccccccgccc cgccgcctcg acctcggccg
     1381 ggacctcgta gccgagctcg ggggtgagcg gctgccagac gatcgtgcag agccggtggc
     1441 gctgcaggtc gcggaacgcc ccgtagtcgg ccacgacgtc gaacgtgtag acggaccgct
     1501 cgaacgcccg gcccggcttg tgccgccggt tctggcgctc ccccacgtag gcggcgaaga
     1561 ggccggcccg gtcggcggca ccgagccggg cgacctgggc ggccacgctg gcctccccct
     1621 cgcggaggtg cgggtaggcg atcgcggtca gcaccttgtc ctcgccggcc gggtcgaact
     1681 cggagaggcg gaccgacggg ccctcctcgg cgccggtgcc gggacccagc accgcgccga
     1741 ccacctcggc ggtctcctcg gcggtacggg cgagatagcg cgaccaggcc acgccgcggt
     1801 cgtcgcggtc gacccgggtg aggaacgccg ggatcaccat gcggagctcg gcgagcagca
     1861 gctcgccgta ggagcgcgcc tcgggcagcg ggtgggccgc gagacggacc agcagcgcct
     1921 cgtacgcctg cccgctcgcg tagatcccga ggttcgaggt ggtggcggcc gggagcaggc
     1981 cgcgcagcgc gtccagggcg cgcgcccgca ccgcccgccg ccaggccggt tcgctggtgc
     2041 ccggctcgtg gggcacgacc cgggcgaggt gctcggtgag cacggggacc agctcggcgt
     2101 aggtctcgaa cagcccgtcg agcacccggc ggtactcggg gccgagctcg gcgtcggcgg
     2161 ccacctcagg gtcgaggtgg taccgccagc ggccgccggg gcggtcggcg taggagacgt
     2221 accgggtcga ctgctccagg taggcggcga gcctgcccca ctcgagcgcc ttggtgagca
     2281 ggttggacgc ctgctcgcag gcgacgtggg cgccgccgag ctgggccacg gagtcgtccc
     2341 cgtactcgtc gaacacgcgc tggtagaggc gggccgccct ggccg
//
LOCUS       DASPYY010000664         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2498849, whole genome shotgun sequence.
ACCESSION   DASPYY010000664 DASPYY010000000
VERSION     DASPYY010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498849"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VEP73_10475"
     CDS             <1..431
                     /locus_tag="VEP73_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HYT26894.1"
                     /translation="SLARLRARALPPFRQAADAGLPGVMVGHLLVTAVDPDRPASLSP
                     VVIGRLLRQEVGFRGLVVTDALEMGAIVRSRGLPEAAELAVAAGSDQVLIGAGYGSVT
                     AVIDRLERAVAAGRLPRARVREAFLRVERFKGQHRWDGCG"
     gene            complement(448..1491)
                     /gene="purM"
                     /locus_tag="VEP73_10480"
     CDS             complement(448..1491)
                     /gene="purM"
                     /locus_tag="VEP73_10480"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HYT26895.1"
                     /translation="MRAGGPEPVPGRGSLTYAASGVDVDAGYRAVAMMRAAVEATHGP
                     RVLAGIGGFAGLWSFDRARWRDPVLASSADGVGTKLLVAQALDRHDTVGVDLVAMVVD
                     DLVVVGAEPLFLLDYLAVGRLDPARAAAVVAGVAEGCRLAGCALLGGETAEHPDAMEP
                     GAYDLAGFAVGVAERDRLLGPDRVRPGDVLLAMASTGLHSNGFSLVRHALAQAGIGYD
                     RVLPELGAPIGEVLLTPTRVYARDVLDLAASGVELHALCHVTGGGLPGNLPRSLPPGL
                     VARVDRASWEPPPIFRVFKDLGGVADAEMARSTNLGVGMVGVLPAAGAARAVAFLAGR
                     GLDAWVMGEVAPA"
BASE COUNT          208 a          636 c          638 g          172 t
ORIGIN      
        1 cgtccctcgc gcggctgcgc gcacgcgccc tgccgccctt ccgccaggcg gccgacgccg
       61 gcctgcccgg ggtgatggtc ggccacctgc tcgtcaccgc ggtcgacccc gaccggcccg
      121 cgagcctgtc gccggtcgtg atcggccggc tgctccgcca ggaggtcggg ttccgcgggc
      181 tggtggtgac cgacgccctc gagatgggcg cgatcgtgcg ctcccgtggc ctgcccgagg
      241 cggccgagct cgccgtggcg gcaggcagcg accaggtgct gatcggcgcc ggctacgggt
      301 cggtcaccgc ggtcatcgac cggctggagc gggcggtggc ggccgggcgc ctcccgcgcg
      361 cgcgcgtcag ggaggcgttc ctccgcgtgg agcgcttcaa ggggcagcac cgctgggacg
      421 gctgcgggta gcggcagccg tcccgggtca ggcgggcgcc acctcgccca tgacccaggc
      481 gtcgaggccc cggccggcca ggaacgcgac ggcgcgcgcg gccccggcgg ctgggagcac
      541 gccgaccatc ccgacgccca gattcgtgga ccgtgccatc tccgcgtcgg ccacgccccc
      601 caggtccttg aagacccgga agatcggggg cggctcccag gaggcccggt cgacccgggc
      661 gaccaggcct ggcggcaggc tgcggggcag attgcctggc aggcccccgc cggtgacgtg
      721 gcagagcgcg tgcagctcca cgccggaggc cgcgaggtcg agcacgtcgc gggcgtagac
      781 gcgggtcggc gtgagcagga cctctccgat cggggcgccc agctcgggca gcacccggtc
      841 gtagccgatc ccggcctggg cgagggcgtg gcggacgagc gagaagccgt tcgagtggag
      901 cccggtcgag gccatcgcga ggagcacgtc tccgggccgc acgcggtcgg ggccgagcag
      961 gcggtcccgc tcggccacgc ccacggcgaa gccggccagg tcgtaggctc ccggctccat
     1021 cgcgtcgggg tgctcggcgg tctcgccgcc gagcagggcg cagccggcca gccggcatcc
     1081 ctcggccacc ccggccacga cggcggccgc ccgcgcgggg tcgagacggc ccacggcgag
     1141 gtagtcgagc aggaacagcg gctcggcccc gaccaccacc aggtcgtcga cgaccatcgc
     1201 gacgaggtcg acgccgaccg tgtcgtgccg gtcgagcgcc tgggcgacga ggagcttcgt
     1261 ccccaccccg tcggccgagc tggccaggac cgggtcccgc caccgcgctc ggtcgaagga
     1321 ccagaggccg gcgaacccac cgatcccagc gagcacgcgg ggcccgtggg ttgcctccac
     1381 cgcagcgcgc atcatggcaa ccgcccggta gccggcgtcg acgtcaacgc ccgacgccgc
     1441 atacgtcagg ctcccacgcc cggggacggg ctccggcccg ccggccctca cgggaccgcg
     1501 ggagccgccc cggcgcgctc gagcaccgac ttgcccggga agctcgagtc gaccgggatc
     1561 gggtagtcgc cggtgaagca cgccgtgcag agcccgcgcc cacccggggc ggccgcctcg
     1621 gccagcgcct cgggcgagag gtagccgagc gagt
//
LOCUS       DASPYY010000665         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2498995, whole genome shotgun sequence.
ACCESSION   DASPYY010000665 DASPYY010000000
VERSION     DASPYY010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498995"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..649)
                     /locus_tag="VEP73_10485"
     CDS             complement(62..649)
                     /locus_tag="VEP73_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT26896.1"
                     /translation="MSDQAPSDALHDPTTEDGSGRQDYPTPGAGPGVRDALENYTLSV
                     NGREREVTEAWLGESLLYVLRERLGLPGAKNACEQGECGSCSVLLDGRLVASCCVMAA
                     DAVGSEIATVEGLSGEGALSDVQRAFVEEGAVQCGFCTTGLVVAIHDVLDRNPRATEL
                     ELREAISGNLCRCTGYGRIFAAVRRVQQERAHART"
     gene            complement(646..1485)
                     /locus_tag="VEP73_10490"
     CDS             complement(646..1485)
                     /locus_tag="VEP73_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYT26897.1"
                     /translation="MQISRPRTLEEAFDALDEMPAAHLLAGGTDFMVEVNFGHRRPPA
                     VVGLRRVEELAGFELTADEVVLRAGLSWTEVETELDEELPALAAAARTVGSPQMRNAG
                     TVGGNVGTASPAGDSLPVLAAMDATVVLVSRGGERRLPLSAFITGVKRTAIGPGEIIR
                     EVRVPRIRGPQHFLKVGTRNAMVISIACAAVVLDTEGRTVRCGLGAVAPTPVRAPAAE
                     SFIAGAIDWDEVRASPEAVARFGELAAAAASPITDHRSTAEYRRHALKVITTRALRRS
                     LAA"
BASE COUNT          193 a          567 c          554 g          213 t
ORIGIN      
        1 gcgcaggacg ctctcgccga cccccggagc gctgcgcttg cgggtgaagg tggtgggcat
       61 gctaggtgcg ggcatgggca cgctcctgct ggactcgacg gaccgccgca aagatccggc
      121 cgtagccggt gcaccggcag aggtttcccg agatcgcctc acggagctcg agctcggtgg
      181 cccgggggtt gcggtcgaga acgtcgtgga tggccacgac gagccccgtc gtgcagaagc
      241 cgcactgcac ggcgccctcc tcgacgaagg cgcgctgcac gtccgacagc gcgccttcgc
      301 ccgacagccc ctcgacggtg gcgatctcgc tgcccaccgc atcggctgcc atgacgcagc
      361 acgaggcgac cagccggccg tccagcagca ccgagcagga gccgcactcg ccctgctcgc
      421 aggcgttctt ggcgccgggg agcccgagcc gctcgcgcag cacgtagagc aggctctcgc
      481 cgagccatgc ctcggtcacc tcgcgctccc gcccgttcac gctcagcgtg tagttctcca
      541 gcgcgtcgcg gacgcccggt cccgcgccgg gcgtcgggta gtcctgacgc ccgctgccgt
      601 cctccgtcgt cgggtcgtgc agcgcgtcac tcggtgcctg gtcgctcatg ctgcgaggct
      661 cctccgcagt gcccgggtgg tgatgacctt cagcgcgtgg cggcggtact cggccgtgct
      721 gcggtggtcg gtgatcgggg aggcggccgc ggccgcgagc tcgccgaagc gggcgacggc
      781 ctcgggcgac gctcgtacct cgtcccagtc gatcgctccg gcgatgaacg actcggccgc
      841 cggtgcgcgg accggggtgg gcgcgaccgc gccgaggccg caccggacgg tccgcccctc
      901 ggtgtccagc acgaccgccg cgcaggcgat cgagatgacc atcgcgttgc gggtgccgac
      961 cttcaggaag tgctgcgggc cccggatccg ggggacgcgg acctcacgga tgatctcgcc
     1021 cggcccgatg gcggtgcgct tcaccccggt gatgaacgcc gacaggggca gccgccgctc
     1081 gccgccgcgg ctgacgagga cgacggtggc gtccatggcg gccagcaccg gcagcgagtc
     1141 cccggccggg cttgcggtgc cgacgttgcc ccccacggtg cccgcgttgc gcatctgcgg
     1201 cgagccgacg gtacgggcgg cggccgcgag cgcgggcagc tcctcgtcca gctcggtctc
     1261 gacctcggtc cagctgaggc cggcacgcag gacgacctcg tcagcggtca gctcgaagcc
     1321 cgccagctcc tcgacgcggc gcagcccgac gaccgcgggc gggcggcgat ggccgaagtt
     1381 cacctcgacc atgaagtccg tgccgccggc cagcaggtgg gcggccggca tctcgtcgag
     1441 cgcgtcgaag gcctcctcca gcgtcctcgg gcggctgatc tgcatggcac ctcgcttccg
     1501 acacctaatc cgtcctggtc gtggtgc
//
LOCUS       DASPYY010000666         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1655856, whole genome shotgun sequence.
ACCESSION   DASPYY010000666 DASPYY010000000
VERSION     DASPYY010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655856"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VEP73_10495"
     CDS             <1..1397
                     /locus_tag="VEP73_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYT26898.1"
                     /translation="GAARPPAAAGGEGPPVPVAPGLVAAAALVRDGAATSTALVGQAL
                     ERIGALDGRLGAFVAVLADRALDEATALDAERAGGRLRGPLHGVPVAVKDLVDVAGAV
                     TAAGSPKLAANLAAADAEVVARLRRAGAVVVGKTRTHEFAYGVRTPPTANPWDPERIP
                     GGSSGGSAAAVAARLVPFAVGSDTAGSIRIPAACCGVVGLKPTHGAVPAAGVWPLSWS
                     CDHVGPIARDVADAALAFSVMAGRPVPPAASRGGVRGLRLGWLVGEEVEALDPGVAGA
                     MGGLRAGLVAAGAAIEEVRLPLGAARAAVGAIVLPELAAAHARLLASTGEDGYGPAVL
                     AAIRLGQAQLASEYLAGLRYRGRFAARVEALLSRHDALVLPTIPVPAPRRDDRTVPVG
                     GVPTTIQAALTALPGAFNCSGSPVVSLPAGLAGGLPVGVSLVGRAGGDGELLRVAASV
                     EVAMPALGPPPACA"
     gene            1472..2251
                     /locus_tag="VEP73_10500"
     CDS             1472..2251
                     /locus_tag="VEP73_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT26899.1"
                     /translation="MTNDGHANQAEPAEDGVEVAGSKPPAGRGAAGRALGRNQASRVG
                     WARTPPARVVRGLVQRGLLVPTLRFISPFTVIGKRNLAQLDGPAVFVANHQSHFDAPV
                     CLAALGGRVRRRLVIAAAADYFYSSALKGAAASLALGTVPFVRTGGGSRESLSMLKDL
                     VHKGWSVLIFPSGTRGTGASGFKKGFAYIAVDAQVPVVPLYLHGLDRIMPKGSFVPLP
                     GGVAVGIGPPLPPGKDYAELVRRAEAGVAEVQAVVKRWEGA"
     gene            2260..3186
                     /locus_tag="VEP73_10505"
     CDS             2260..3186
                     /locus_tag="VEP73_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HYT26900.1"
                     /translation="MPHNPFGNPLFVPVFLRVVLLIVIGLAGVLLAQRRSLTVLRRST
                     FFTRTRTWFWIGPLFLVCAFTGGFVAFLLATFVVIQGLSEFVRIAGIDRRYALLLMLW
                     SEIGLLVASLARDYFLLLPFGFFVLLTLLPVLSGEVRDAHRQLGDTLFGYVYIGLPLA
                     FIVLASDTPRWGLEFLVIVTVSVAMSDIAAYAVGAALKGPKMIPSINPTKTWSGALGN
                     LLGAAVGVALLSIAIPGEWTVAGVVALVVMVAVGSAWGDVTDSFVRRTFHVEASSTVL
                     FGFGGILDRVDSLLMALPLCYYALLLADRLFG"
     gene            complement(3294..>4566)
                     /locus_tag="VEP73_10510"
     CDS             complement(3294..>4566)
                     /locus_tag="VEP73_10510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HYT26901.1"
                     /translation="VIHTSTPPLTAAFHDTFFPSLPGPDFDQVFPVGAPDFKNGCHGN
                     GTGMSGPCAAANWSGEATLDIEWAYAIAPKAHIVLIGVPPAETEGVQGFPNLFKALRQ
                     AIDRYPAGTVVSQSFGVTEQTFGGAAATQTARFDAVYQAAAAKGDTVLASSGDNGTTG
                     VAKQHADSTTYPFPTAGWPASSPWVTAVGGTQLQYGWTWSPTSNVPFLPNGDYNPAYF
                     AFANGGASEPVWNESWLPAATGGGPSAIYPRPAYQTAQAAVIGRDARGVPDLSWNAAV
                     NGGVLVWITAFPGYQRAGWHVYGGTSASSPQVAGLVALANEQQAKAGKAPLGFLDPLL
                     YKVGDQAGSATAAFGGGGAFRDIVPHTYGTASSGVLDDNRLWQYNPDGSVSPGPVPGW
                     PTLPGWDMTTGFGSPRAAAFVAAVRAAKNTP"
BASE COUNT          476 a         1690 c         1735 g          665 t
ORIGIN      
        1 ggggcgcggc gcggccgccg gccgcggccg gcggggaggg cccgccggtc cccgtggcgc
       61 ccgggctcgt cgcggcggcc gcgctcgtgc gcgacggggc ggcgaccagc acggccctgg
      121 tcggtcaggc cctggagcgg atcggggcgc tcgacggtcg gcttggcgcg ttcgtcgcgg
      181 tgctcgccga ccgggcgctc gacgaggcga ccgccttgga cgctgagcgt gccggcggcc
      241 ggctccgagg cccgctgcac ggcgtcccgg tcgccgtcaa ggacctcgtc gacgtggccg
      301 gtgccgtcac cgcggccggg tcgccgaagc tggccgcgaa cctcgccgcc gcggacgccg
      361 aggtcgtcgc ccgcctccgg cgggcgggcg cggtggtggt gggcaagacc cgcacccacg
      421 agttcgccta cggggtccgc accccaccga ccgcgaaccc ctgggacccg gagcggatcc
      481 cgggcgggtc cagcggcggc tcggccgccg ccgtcgccgc ccggctggtc ccgttcgccg
      541 tcggcagcga cacggccggc tcgatccgca tcccggcggc ctgctgcggc gtggtcggtc
      601 tcaagccgac ccacggcgcg gtcccggccg ccggcgtctg gccgctgtcg tggtcctgcg
      661 accacgtggg gccgatcgct agggacgtcg ccgacgccgc gctcgcgttc tcggtcatgg
      721 ccggccggcc cgtcccgccg gcggcctccc ggggcggcgt acgcggcctc cgcctcggct
      781 ggctcgtggg cgaggaggtc gaggcgctcg accccggggt cgccggggcg atgggcgggc
      841 tgcgcgctgg tctcgtcgcc gcgggcgcgg ccatcgagga ggtccggctg cccctcggcg
      901 ccgcgcgcgc ggcggtgggc gcgatcgtgc tccccgagct ggccgccgcc cacgcccgcc
      961 tgctcgccag caccggcgag gacggctacg gcccggcggt actcgccgcg atccggctcg
     1021 gccaggccca gctcgccagc gagtaccttg ccggcctgcg ctaccgcggc cgcttcgcgg
     1081 cccgggtcga ggcgctgctc tcgcggcacg acgccctggt gctgccgacc atccccgtcc
     1141 cggcgccgcg acgcgacgac cgcacggtgc ccgtcggggg cgtgcccacc acgatccagg
     1201 ccgccctcac ggcgctgccc ggcgcgttca actgctcggg ctccccggtc gtgtcgctcc
     1261 ccgccggcct cgcgggggga ctgcccgttg gcgtgtcgct ggtcgggcgg gccggcgggg
     1321 acggcgagct gctgcgggtg gccgcctcgg tcgaggtggc catgcccgcg ctcggccccc
     1381 cgcccgcttg tgcgtaactg cccagcacgt tgtcggtctg gttcaatgcg atccaccgat
     1441 ccaccgaggg cgggcggcgc gggagggtga cgtgacgaac gacgggcacg cgaaccaggc
     1501 cgagccggcc gaggacgggg tggaggtcgc cgggagcaag ccgccggcgg gccgcggggc
     1561 ggccggccgg gccctcggcc gcaaccaggc gtccagggtg ggctgggcgc ggacgccgcc
     1621 ggccagggtg gtccgggggc tcgtccagcg ggggctcctc gtcccgacgc tccgcttcat
     1681 ctcgccgttc acggtcatcg gcaagcgcaa cctcgcccag ctcgacgggc ccgcggtgtt
     1741 cgtcgccaac caccagagcc acttcgacgc cccggtctgc ctggccgccc tgggcggccg
     1801 cgtccggcgc cgcctggtga tcgcggccgc ggccgactac ttctacagct cggccctgaa
     1861 gggggcggcc gcgtcgctgg cgctcggcac ggtgccgttc gtgcgcacgg gcgggggcag
     1921 ccgcgagtcc ctgtcgatgc tgaaggacct cgtccacaag ggctggtcgg tgctcatctt
     1981 cccgtcgggg acccgcggga cgggcgcgag cggcttcaag aagggcttcg cctacatcgc
     2041 ggtcgacgcg caggtgcccg tcgtccccct ctacctgcac ggactggacc ggatcatgcc
     2101 gaagggctcg ttcgtccccc tgccaggcgg ggtcgccgtg ggcatcggcc ctcccctccc
     2161 cccgggcaag gactacgcgg agctggtccg cagggccgag gccggggtcg ccgaggtcca
     2221 ggcggtcgtc aagcgctggg aaggggcgta acgccctcga tgccccacaa ccccttcggc
     2281 aacccgctgt tcgtccccgt cttcctccgc gtcgtcctgc tgatcgtcat cggcctggcg
     2341 ggcgtcctgc tggcccagcg gcggtcgctc accgtgctgc gccggtccac cttcttcacc
     2401 cgcacccgca cgtggttctg gatcgggccg ctgttcctcg tctgcgcgtt caccggcggc
     2461 ttcgtcgcct tcctgctggc gaccttcgtg gtcatccagg ggctgtccga gttcgtgcgg
     2521 atcgccggca tcgaccgccg gtacgcgctc ctgctgatgc tctggtcaga gatcggcctg
     2581 ctggtggcct cgctcgcccg cgactatttc ctcctgctgc cgttcgggtt cttcgtcctg
     2641 ctcacgctcc tgcccgtcct gtccggcgag gtgcgtgacg cccacaggca gctcggcgac
     2701 accctgttcg gctacgtcta catcgggctg ccgctggcgt tcatcgtcct ggcgagcgac
     2761 accccgcgct gggggctcga gttcctcgtg atcgtgacgg tgtcggtggc catgtccgac
     2821 atcgcggcct acgcggtcgg cgccgccctc aagggcccca agatgatccc gagcatcaac
     2881 ccgaccaaga cctggtccgg ggcgctcggg aacctgctcg gggcggccgt cggggtcgcg
     2941 ctgctgtcca tcgcgatccc cggcgagtgg accgtggccg gcgtggtcgc gctcgtcgtc
     3001 atggtcgcgg tcggcagcgc ctggggcgac gtgaccgata gcttcgtcag gcgcacgttc
     3061 cacgtggagg cctcgagcac ggtgctgttc ggcttcggag ggatcctgga ccgggtcgac
     3121 tcgctgctca tggccctgcc gctgtgctac tacgcgctgc tcctggcgga caggctgttc
     3181 ggctgacgag acagggcagg cgcgtgggcc gcgcatcgtc gggcacgtca ccgcggcgag
     3241 gccccgccct gtgcgcaggg cggggcctcg tgccagccgg tgtgtcgtgc gggtcagggc
     3301 gtgttcttgg cggcgcggac ggcggcgacg aaggcggccg cgcggggcga gccgaacccg
     3361 gtggtcatgt cccagccggg cagggtcggc cagccgggca ccggcccggg cgagacgctg
     3421 ccgtccgggt tgtactgcca cagccggttg tcgtcgagca cgccgctgct cgccgtgccg
     3481 taggtgtgcg gcacgatgtc gcggaacgcc ccaccgccgc cgaacgcggc cgtggccgag
     3541 cccgcctggt caccgacctt gtacagcagc gggtcgagga agccgagcgg cgccttgccc
     3601 gccttggcct gctgctcgtt ggccagcgcg acaagacccg cgacctgcgg cgaggacgcg
     3661 ctggtcccgc cgtagacgtg ccacccggcc cgctggtagc ccgggaaggc ggtgatccac
     3721 accagcacgc cgccgttgac cgcggcgttc cacgacaggt caggcacacc gcgcgcgtcg
     3781 cgcccgatga ccgcggcctg cgccgtctgg tacgcgggcc gcgggtagat ggcgctcggg
     3841 ccgccgccgg tggctgccgg cagccaggac tcgttccaca ccggctcgga cgcgcccccg
     3901 ttggcgaagg cgaagtacgc cgggttgtag tcgccgttgg gcaggaacgg cacgttgctg
     3961 gtcgggctcc aggtccagcc gtactgcagc tgggtgccgc cgaccgcggt gacccagggg
     4021 ctggacgccg gccagccggc cgtcgggaac gggtaggtgg tggagtcggc gtgctgcttg
     4081 gccaccccgg tggtgccgtt gtcgcccgag gaggcgagca cggtgtcccc cttggccgcg
     4141 gccgcctggt agaccgcgtc gaaccgggcg gtctgggtcg cggcggcgcc gccgaaggtc
     4201 tgctcggtca ccccgaagct ctgcgagacg acggtgccgg ccgggtagcg gtcgatggcc
     4261 tgccgcaggg ccttgaagag gttggggaag ccctgcacgc cctcggtctc ggccgggggc
     4321 acgccgatga gcacgatgtg tgccttgggc gcgatcgcgt aggcccactc gatgtccagg
     4381 gtcgcctcgc ccgaccagtt cgccgccgcg cacggcccgg acatgccggt gccgttgccg
     4441 tggcagccgt tcttgaagtc gggggcgccg accgggaaga cctggtcgaa gtcggggccg
     4501 ggcaggctcg ggaagaaggt gtcgtggaac gccgcggtca acggcggcgt gctggtgtgg
     4561 atcacc
//
LOCUS       DASPYY010000667         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_813222, whole genome shotgun sequence.
ACCESSION   DASPYY010000667 DASPYY010000000
VERSION     DASPYY010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813222"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..475)
                     /locus_tag="VEP73_10515"
     CDS             complement(92..475)
                     /locus_tag="VEP73_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HYT26902.1"
                     /translation="MSTGPANAARRDEASAPWFDGLADGVLLIRRCPRCGLHSRPDTA
                     ACPACHAEGLEWVPAAGRGTVVCVIVEHREQDEVALGLVELDEGPWLAARITDRARAT
                     PGARVELVVVTPDEGEPFPAFAPVG"
     gene            complement(472..>1608)
                     /locus_tag="VEP73_10520"
     CDS             complement(472..>1608)
                     /locus_tag="VEP73_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYT26903.1"
                     /translation="VGAGLTEVGKVYGRSARSLAAEAVRLAVADAGLTLGQIDGLLIN
                     AGKTSGLDLSLARHLGLRDLRMLSEVNALGASAGAMVAQAAMAVLSGAASAVACVFAD
                     TPRQPGAAAGAAYQQTSLQATGFRSAETAAGFRSVNHRYALAASRYMHTFGVTQDQLG
                     AVAVGQRAWAVHNPAAQMRTPLTLEEYHRSRWIVEPLHLLDCCLVSNGGAAVVVTTTE
                     RAGALRQPPVQVLGWGQAHPGTLWQRGSDFGLVTGAALSGPAALKMAGLAPADIDLRE
                     IYDCYTYTVLVTLEDYGFCGKGEAGELAASGALAPGGALPTNTGGGQLSAYYLWGMTP
                     LMEAVTQVRGQAGARQTARHDTAIVSGNGGLLDHHATLVMGAIR"
BASE COUNT          213 a          561 c          615 g          219 t
ORIGIN      
        1 agcctccttg ggagttaagg taaatgaccg tacgtacggt cagtataaag ctcagttgcg
       61 ggcgggacaa gggtgaggtt ctccgcgcct gtcagccgac gggcgcgaag gcggggaagg
      121 gctcgccctc gtccggggtc accacgacga gctcgacccg ggcgcccggg gtcgcacgcg
      181 cgcgatcggt gatccgggcg gcgagccacg gcccttcgtc cagctcgacg aggccgagcg
      241 ccacctcgtc ctgttcgcgg tgctcgacga tcacgcacac gacggtgccg cggccagcgg
      301 cgggaaccca ttcgagccct tcagcgtggc aggccgggca cgcggcggtg tcggggcggc
      361 tgtgcaggcc gcaccggggg cagcggcgga tcagcaggac gccgtcggcg agcccgtcga
      421 accagggcgc ggaggcctcg tcgcgacggg ccgcgttggc cggtccagtg ctcatcggat
      481 cgctcccatc acgagagtgg cgtggtggtc gagcaggccg ccgttgccac tgacgatggc
      541 ggtgtcgtgg cgtgccgtct ggcgggcgcc cgcttgaccg cggacctggg tgacggcctc
      601 catgagcggg gtcatgcccc acaggtagta ggcggagagc tgcccgccgc cggtgttggt
      661 cggcagcgcg ccgccgggcg cgagtgcgcc gctcgccgcg agctcgcccg cctcgccctt
      721 gccgcagaag ccgtagtcct cgagcgtgac caggacggtg taggtgtagc agtcgtagat
      781 ctcgcgcagg tcgatgtcgg ccggggcgag gccggccatc ttcagggcgg ccgggccgga
      841 cagtgcggcg ccggtgacga ggccgaagtc gctgccgcgc tgccacagcg tgcctggatg
      901 ggcctgaccc caacccagga cctgcacggg tggctgccgc agggcgcccg cgcgttccgt
      961 cgtggtgacc acgaccgcgg cgcccccgtt ggagaccagg cagcagtcga gcaggtgcag
     1021 cggctcgacg atccagcggg agcggtggta ctcctcgagc gtgagcggcg tgcgcatctg
     1081 cgctgccggg ttgtggaccg cccatgcccg ctgtccgacc gccaccgcac ccagctggtc
     1141 ctgggtgacg ccgaaggtat gcatgtagcg gctcgcggcg agcgcgtacc ggtggttgac
     1201 gctgcggaac ccggccgcgg tctccgcgct gcggaatccg gtggcctgca gcgacgtctg
     1261 ctggtaggcc gcgccggccg ccgcacctgg ttgacgcggg gtgtcagcga acacgcaggc
     1321 cacggccgac gcggcgccgg agaggaccgc catggccgcc tgcgccacca tggctcccgc
     1381 ggaggcgccg agcgcgttga cctcgctgag catcctcagg tcgcgcaggc cgaggtggcg
     1441 ggcgagcgac aggtcgaggc cgctggtctt gcccgcgttg atcagcagcc cgtcgatctg
     1501 ccccagggtc agccccgcgt cggcgacggc cagccggacc gcttcggcgg cgagcgaccg
     1561 ggcgctgcgc ccgtacacct tgccgacctc ggtcaggccc gcccccac
//
LOCUS       DASPYY010000668         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2077984, whole genome shotgun sequence.
ACCESSION   DASPYY010000668 DASPYY010000000
VERSION     DASPYY010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077984"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1105
                     /locus_tag="VEP73_10525"
     CDS             212..1105
                     /locus_tag="VEP73_10525"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYT26904.1"
                     /translation="MRRGLRRVGVVVHTGRPPALALGKELARWSADHGVELRTLDEER
                     GCLGEGVPCASPDEFTEGLDLVVVLGGDGTLLRAIRLALDDQVPVLGVNLGRLGFLTE
                     VEAVDMPAALDAVWEGRYQVERRMTLAARTEVDDRAVAEDVAVNDVVLEKAARERLAG
                     VAAYVDGRLFARYAADGVMVASPTGSTAYSFSAGGPIVSPRVHALLLTAIAPHMVFNR
                     TLVLHPDEVVRLEVLKGGPVVESLDGRAMRELPPGASVEVRRGDHDALLVRIGEPDFY
                     GLVRSKFRLPDAGEARGGLDD"
     gene            1098..>1968
                     /locus_tag="VEP73_10530"
     CDS             1098..>1968
                     /locus_tag="VEP73_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HYT26905.1"
                     /translation="MTRVGSVPSGAGGREGREPGGPGPPAHQGLTAAEVADRVARGLA
                     NDVPRAPTRTVGQILRANVLTRFNLLLGSLLAVILVVGPLQDALFGLVIVANTLVGVV
                     QELRAKRTLDRLAVVAAPKAQVVRDGRPAELAVGEVVLGDLLLAGPGDQVVVDGEVVE
                     ATALEVDESLLTGESEPVGKGPGEEVLSGSFVAAGSGAYRAARVGREAYAAGLSEEAR
                     RFSLARSELRAGIDRIVSLIAWVLVPTAALLLASQLRAHSSVREGLRGAVAGTVAMVP
                     EGLVLLTSVAFAVS"
BASE COUNT          198 a          723 c          799 g          248 t
ORIGIN      
        1 cgtcgtcccg gccgggccca gccggcaacg tcgagctctt cctgcacctg cgcgcgggcg
       61 cggggccgct cgccgagcgg gcgctggccg acgccgtggc cgagggcgcc agcctgccgg
      121 gcgcggcccg atgaccgcgg cgggcgccgc ggcgggggcg gccgccggcg ccgcggccga
      181 tcggtcggcg gggggcttgc acgacgggcg cgtgcgccgc gggctgcggc gcgtcggggt
      241 ggtcgtccac accggccggc cgcccgcgct ggcgctcggc aaggagctcg cccgctggtc
      301 ggccgaccac ggcgtcgagc tccggaccct cgacgaggag cggggctgcc tcggcgaggg
      361 ggtgccgtgc gcgagccccg acgagttcac cgaggggctg gacctggtgg tggtgctcgg
      421 cggcgacggc accctgctgc gcgccatccg gctcgccctg gacgaccagg tgcccgtgct
      481 gggggtgaac ctcgggcggc tcggcttcct caccgaggtg gaggccgtcg acatgcccgc
      541 cgccctcgac gcggtgtggg agggccgcta ccaggtcgag cggcgcatga ccctggccgc
      601 ccggaccgag gtcgacgacc gcgccgtcgc cgaggacgtc gcggtcaacg acgtcgtgct
      661 cgagaaggcg gcgcgggagc ggctggccgg cgtcgccgcg tacgtggacg ggcggctctt
      721 cgcccgctac gcggcggacg gggtcatggt cgcgtcgcca acgggctcga ccgcctacag
      781 cttctcggcc ggcgggccga tcgtctcccc ccgggtgcac gcgctgctgc tgaccgcgat
      841 cgcgccgcac atggtgttca accgcacgct ggtcctgcac cccgacgagg tggtcaggct
      901 ggaggtgctc aaaggcggcc cggtggtgga gagcctcgac ggccgggcca tgcgggagct
      961 gccgcccggt gccagcgtcg aggtccgccg gggcgaccac gacgcgctcc tcgtccggat
     1021 cggcgagccg gacttctacg gcctggtgcg ctcgaagttc cgcctgcccg acgccggcga
     1081 agcgcgcggc gggctcgatg actagggtcg ggagcgtccc gtccggtgca ggcggccggg
     1141 aaggccgcga gcccggcggc cccgggcccc ccgcccacca gggcctgacc gcggcggagg
     1201 tcgccgaccg ggtcgcgcgc ggcctcgcca acgacgtgcc gcgggcgccg acccggacgg
     1261 tcgggcagat cctgcgggcg aacgtgctga cccgcttcaa cctgctgctc ggctcgctgc
     1321 tcgccgtcat cctggtcgtc gggccgctcc aggacgcgct gttcgggctg gtcatcgtcg
     1381 ccaacacgct ggtgggcgtc gtccaggagc tgcgcgccaa gcgcaccctc gaccgcctcg
     1441 cggtggtggc cgccccgaag gcgcaggtgg tgcgcgacgg ccggccggcg gagctcgcgg
     1501 tcggcgaggt cgtgctcggc gacctgctcc tggccggtcc gggcgaccag gtcgtggtcg
     1561 acggcgaggt ggtcgaggcg accgccctgg aggtggacga gtcgctgctc accggcgagt
     1621 ccgagccggt cggcaagggc ccgggcgagg aggtgctctc cggcagcttc gtcgcggccg
     1681 ggtcgggcgc ctaccgggcc gcgcgggtcg gccgcgaggc gtacgcggcg gggctctccg
     1741 aggaggcgcg gcggttctcc ctggcccggt cggagctgcg cgccgggatc gaccgcatcg
     1801 tcagcctcat cgcgtgggtc ctggtcccga ccgcggcgct gctgctcgcg agccagctcc
     1861 gcgcccactc gagcgtccgc gagggcctgc ggggcgcggt ggcgggcacg gtcgcgatgg
     1921 tcccagaggg gctcgtcctg ctcaccagcg tcgccttcgc ggtgagcg
//
LOCUS       DASPYY010000669         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1516904, whole genome shotgun sequence.
ACCESSION   DASPYY010000669 DASPYY010000000
VERSION     DASPYY010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516904"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="nrfD"
                     /locus_tag="VEP73_10535"
     CDS             <1..767
                     /gene="nrfD"
                     /locus_tag="VEP73_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HYT26906.1"
                     /translation="GNHRLARAALTTGAASLAASPVLLTADLGRPERFHHMLRVFRPT
                     SPMNLGSWLLAAISPALLGAAVADRLGITPRLARTAEGVAAVLGPGLATYTAVLLADT
                     AVPVWHEAGRELPFVFAGSAAAAAGGAAAILTPPAEAGPARRLAVAGALVELGAAEVM
                     QRRLGELAEPYGKEPARRYARLARACGGAGAAVLALAGRRRPAAVLGGALLLAGSACQ
                     RLAVHKAGIESARDPKYVVKQQRERLEANGGPPPRG"
     gene            complement(809..>2736)
                     /locus_tag="VEP73_10540"
     CDS             complement(809..>2736)
                     /locus_tag="VEP73_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT26907.1"
                     /translation="CYEEEAGLAYGPFIDVLRALAAGGWRDGESRGWDEGLAGHWLAE
                     AARLVPELAEGRAGLPPVPAPDSPGAQGRFLEGLSQVLLAALRGPVPGVLLLDDLHWV
                     DEASMDVLTYLVRRLRGRPLAIVATWRGEQVARTHRLRRLVAEAERSGAATVLRLARL
                     TEPEVAELVRAVAPASSSASGDLWRESEGLPFFLVEYLAAMAAGDGARGPAGPLPGGV
                     RELVRSRLASVSGTAWQVLTTAAVIGRSFDLETVREASGRGDEETVAALEELTVRGLV
                     GEVATAGAAGAGIPRAADGAGAVVYDFRHDKVRGLVYEEASLARRRLLHRRVAEALAG
                     RSRGQRGAAAALVGHHLQLAGEEREAALAFEVAGAHARTLYANREAVGHYRAALALGH
                     PDAAGIHEAIGDLETLLGDYDAARAGYETAAAHREPGEAWAVEQKLGGLHHRRGDWDA
                     AEGHFEAALAALGEAGSPGRRAGVLADWSGTALRRGRPDRALELASRALELAGADGDP
                     RALAQAHNSLGVVRSRLGDDDAARRHLEESLALAAALPDPGARVAAGNNLALALGRAG
                     RVDEAIRLEEAALDLCAAQGDRHREAALHNNLADLLHAAGRTEEAMAHVKLGVAIFAE
                     VGDADPFQPEIWKLVEW"
BASE COUNT          280 a         1113 c         1048 g          295 t
ORIGIN      
        1 ctggcaacca ccggctcgcc cgcgccgcgc tcaccacggg cgcggcctcg ctcgctgcca
       61 gcccagtgct gctcaccgcc gacctcggcc ggccggagcg cttccatcac atgttacgag
      121 tctttcggcc gacctcaccg atgaacctgg ggagctggct gctcgccgcg atcagcccgg
      181 cgctgctcgg cgcggccgtc gccgaccggc tcggcatcac gcccaggctg gcccgcaccg
      241 ccgagggcgt cgccgccgtt cttggcccgg gcctcgccac ctacaccgcc gtactgctcg
      301 cggacaccgc cgtgccggtc tggcacgagg cgggccgcga gctgccgttc gtgttcgccg
      361 gcagcgcggc cgcggccgcg ggcggggcgg ccgccatcct gaccccgccg gccgaggcgg
      421 gaccggcccg gcgcctcgcc gtcgccggcg cactcgtcga gctcggcgcc gccgaggtca
      481 tgcagcgccg gctgggggag ctggccgagc cgtacgggaa ggagccggcg cggcggtacg
      541 cgaggctcgc ccgggcgtgc ggcggggctg gcgcggccgt gctggcgctc gccggccgcc
      601 gccggccggc ggccgtgctc ggcggcgcgc tgctgctcgc cggctcggcc tgccagcgcc
      661 tggccgtcca caaggcgggc atcgagtccg cccgcgaccc caagtacgtc gtgaagcagc
      721 agcgcgagcg cctcgaggcg aacggcgggc cgcccccgcg cggctgacct ggcgcccgcc
      781 ctcgcgccgc tgacccgggt cgccgcggtc accattcgac cagcttccag atctccggct
      841 ggaacgggtc ggcgtcgccg acctcggcga agatggccac gcccagcttc acgtgggcca
      901 tggcctcctc ggtgcggccg gcggcgtgga gcaggtcggc caggttgttg tgcagtgccg
      961 cctcgcggtg ccggtcgccc tgggcggcgc acaggtcgag ggccgcctcc tcgagccgga
     1021 tggcctcgtc gacccggccg gcccgcccca gggccagcgc gaggttgttc ccggcggcga
     1081 cgcgggcgcc cgggtcgggc agggccgcgg ccagggccag gctctcctcc aggtgccgcc
     1141 gggcggcatc gtcgtcaccg aggcgcgacc ggaccacccc gagcgagttg tgggcctgcg
     1201 cgagcgcccg cgggtcgccg tccgcgcccg cgagctcgag ggcccggctg gcgagctcga
     1261 gggcgcggtc cggccggccc cggcgcagcg ccgtcccgct ccagtcggcg agcacgccgg
     1321 cgcgcctgcc cgggctcccc gcctcgccga gggcggccag ggccgcctcg aagtgcccct
     1381 cggccgcgtc ccagtcgccg cggcggtgat ggaggccgcc gagcttctgc tccaccgccc
     1441 aggcctcgcc cggctcgcgg tgcgcggcgg cggtctcgta gcccgcccgg gccgcgtcgt
     1501 agtcgccgag cagcgtctcg aggtccccga tcgcctcgtg gatgcctgcc gcgtcgggat
     1561 ggccgagggc cagcgccgcc cggtagtgcc cgaccgcctc ccggttggcg tacagggtgc
     1621 gggcgtgggc gcccgccacc tcgaacgcga gcgccgcctc gcgctcctcg cccgcgagct
     1681 ggaggtggtg gccgaccagc gcggccgcgg cgccgcgctg gccgcgggag cggccggcga
     1741 gcgcctcggc gacccgccgg tgcagcaggc gccggcgcgc gaggctcgcc tcctcgtaga
     1801 cgaggccgcg gaccttgtcg tggcggaagt cgtagacgac cgccccggca ccgtccgcgg
     1861 cgcgcgggat cccggcgccg gccgcgcccg ctgtcgcgac ctcgccgacc aggccgcgca
     1921 cggtcagctc ctcgagcgcg gcgaccgtct cctcgtcgcc ccggccgctc gcctcccgca
     1981 cggtctccag gtcgaacgag cgcccgatca cggcggcggt ggtcaggacc tgccaggcgg
     2041 tcccgctcac ggaggcgagc cgggagcgca cgagctcgcg caccccgcct ggcagcgggc
     2101 cggccgggcc gcgggcgccg tcgcccgcgg ccatggcggc gaggtactcc accaggaaga
     2161 acgggaggcc ctcgctctcc cgccagaggt cgccggacgc gctcgagctg gccggggcga
     2221 ccgcgcggac cagctcggcg acctccggct cagtgaggcg ggcgagccgg aggacggtcg
     2281 cggcgcccga ccgctcggcc tcggcgacga gccgccgcag gcggtgggtc ctcgccacct
     2341 gctcgccgcg ccaggtggcg acgatcgcga gcggccggcc ccgcagccgg cggaccaggt
     2401 aggtgagcac gtccatggac gcctcgtcga cccagtgcag gtcgtcgagg agcagcacgc
     2461 cgggcacggg cccgcgcagg gcggcgagca gcacctggct caggccctcg aggaagcggc
     2521 cctgcgcgcc agggctgtcc ggcgccggga ccggcggcag gccggcgcgt ccctcggcca
     2581 gctcgggcac gagccgggcg gcctcggcca gccagtgccc ggccagaccc tcgtcccagc
     2641 cccgggactc gccgtcgcgc cagccccccg cggccagcgc gcgcagcacg tcgatgaacg
     2701 ggccgtatgc caggccggcc tcctcctcgt agcagc
//
LOCUS       DASPYY010000670         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2078489, whole genome shotgun sequence.
ACCESSION   DASPYY010000670 DASPYY010000000
VERSION     DASPYY010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078489"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="obgE"
                     /locus_tag="VEP73_10545"
     CDS             complement(<1..1231)
                     /gene="obgE"
                     /locus_tag="VEP73_10545"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYT26908.1"
                     /translation="MFVDEATVELAAGRGGDGVTSFHREKYRPRGGPDGGDGGCGGDV
                     VLVADPGVATLVEYHFHPHQRADRGRHGQGSNRKGAAGEDRVLPVPVGTVVRDEHGKL
                     LADLAAPGTRFVAAQGGRGGRGNAILSGPGRRVAHFHERGEPGRQRRLALELRMLADA
                     GLVGFPNAGKSSIVGWASAARPKVADYPFTTLEPVLGVVRVGEHDFVMADVPGLIPGA
                     AAGKGLGHRFLRHVTRSAVLVHVLDLVPLDAGRDPEADLAALEAELAAYDPDLAGRPA
                     LVVANKVDLPEGRARLPEAAAAAARRELAFYPVSAATGEGMQAFLYALGAAVAEARAD
                     RPVPERQEVVTVEPEVPVEVVRDLGGRFRVLGDRPERWVAMTDLGNPEAVGYLQRRLR
                     RAGVEDLLLAAGARPGDD"
     gene            complement(1345..1605)
                     /gene="rpmA"
                     /locus_tag="VEP73_10550"
     CDS             complement(1345..1605)
                     /gene="rpmA"
                     /locus_tag="VEP73_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYT26909.1"
                     /translation="MAHKKGASSSRNGRDSAAKRLGVKRYAGQVVTAGTILVRQRGTH
                     IHPGANVGRGGDDTLFAKAGGRVVYHQARGRRLASIDISDDA"
     gene            complement(1607..1969)
                     /gene="rplU"
                     /locus_tag="VEP73_10555"
     CDS             complement(1607..1969)
                     /gene="rplU"
                     /locus_tag="VEP73_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYT26910.1"
                     /translation="MYAVIRTGGKQYKVKVGDVIDVERLAGDDGASIELAPLLVVDDE
                     GTVTSQAGDLTGALVTATVVDQHRGRKIRVYTYKNKTGQQRHQGHRQSLTRLKVEKID
                     LPGKQGKAAAGHSTEQEN"
BASE COUNT          236 a          781 c          787 g          281 t
ORIGIN      
        1 cgtcgtcccc tgggcgtgcc cccgccgcga gcagcaggtc ctcgaccccg gcacggcgca
       61 ggcggcgctg gaggtacccg accgcctccg ggttgcccag gtcggtcatt gcgacccacc
      121 gctcggggcg gtcgccgagc acccggaagc ggccgcccag gtcgcgcacg acctcgaccg
      181 gcacctccgg ctcgaccgtg accacctcct gccgctcggg taccggccgg tccgcccgcg
      241 cctcggcgac ggcggcgccg agcgcgtaga ggaacgcctg catgccctcg ccggtcgcgg
      301 ccgagacggg gtagaaggcc aactcgcgtc gggcggcggc cgcggccgcc tcgggcagcc
      361 gcgcccggcc ctccggcagg tcgaccttgt tcgcgaccac gagggcgggc cggccggcga
      421 ggtcagggtc gtaggcggcc agctccgcct cgagcgcggc caggtcggcc tcgggatccc
      481 tgccggcgtc cagcgggacg aggtcgagga cgtggacgag gaccgccgag cgggtcacgt
      541 ggcgcaggaa ccggtgcccg aggcccttgc cggcggcggc ccccgggatc agcccgggga
      601 cgtcggccat gacgaagtcg tgctcgccga cgcggaccac gccgaggacc ggctcgaggg
      661 tggtgaaggg gtagtcggcc accttgggcc gggccgccga tgcccacccg acgatgctcg
      721 acttcccggc gttggggaac ccgaccagcc cggcgtcggc gagcatgcgc agctcgagcg
      781 cgagccggcg ctggcggccc ggctcccccc gctcgtggaa gtgggcgacc cgccggcccg
      841 ggccggacag gatggcgttg ccccgtccgc cccggccgcc ctgcgcggcg acgaagcgcg
      901 tccccggggc ggccaggtcg gcgaggagct tgccgtgctc gtcgcggacc accgtcccca
      961 ccgggacggg gaggacccgg tcctcgccgg cggcaccctt gcggttgctg ccctgcccgt
     1021 gcctgccccg gtccgcccgc tggtgggggt ggaagtggta ctcgaccagg gtggccacgc
     1081 cagggtcggc gaccagcacg acgtcgccgc cgcagccgcc gtcgccgccg tccgggccgc
     1141 cccgggggcg gtacttctcc cggtggaagg aggtgacgcc gtcgccgccg cggccggcgg
     1201 cgagctcgac cgtggcctcg tcaacgaaca ccgctcacgt cctcatggct cccggacacg
     1261 gcggcgaagg ccaggaggcc acccggcccc cctgcctgca cgacggcggc cggcagccct
     1321 cggggcagcc ggcgccccgg cccgctacgc gtcgtcgctg atgtcgatgc tggccagcct
     1381 gcggccgcgt gcctggtggt agacgacccg cccgccggcc ttggcgaaca gggtgtcgtc
     1441 gccgccccgg ccgacgttcg cgcccgggtg gatgtgggtg ccgcgctggc ggacgaggat
     1501 cgtgcccgcg gtcaccacct ggccggcgta ccgcttgacc ccgagccgct tggcggccga
     1561 gtcgcggccg ttgcggctgg aggaggcacc cttcttgtgg gccatgtcag ttctcctgct
     1621 ccgtgctgtg accggccgcg gccttcccct gcttgcccgg caggtcgatc ttctcgacct
     1681 tgagcctggt cagcgactgg cggtggccct ggtggcgctg ctggccggtc ttgttcttgt
     1741 aggtgtagac gcggatcttc ctgccgcggt gctggtccac gaccgtggcg gtgacgagtg
     1801 cgccggtcag gtcgccggcc tgcgaggtca ccgtgccctc gtcgtcgacc accagcagcg
     1861 gtgcgagctc gatgctggcg ccatcgtcgc cggcgagccg ctcgacgtcg atgacgtcgc
     1921 cgaccttgac cttgtactgc ttcccgccgg tgcggatgac ggcgtacacg tggcgacagt
     1981 cctttcatgc aagtcatgtc atgcagaggg gatgcgggca cctcgcggtg agcgcgggcg
     2041 cgccagccgc caagggtagt ggggggacgg cgagcgcgtc aacct
//
LOCUS       DASPYY010000671         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2500200, whole genome shotgun sequence.
ACCESSION   DASPYY010000671 DASPYY010000000
VERSION     DASPYY010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500200"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VEP73_10560"
     CDS             <1..196
                     /locus_tag="VEP73_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26911.1"
                     /translation="DVDAELAAVWRGYDHGVVTEEEVHQRLDELERLLWRQVFQRLRR
                     VQPPEPAATAGGAPERVPAT"
     gene            complement(306..1484)
                     /locus_tag="VEP73_10565"
     CDS             complement(306..1484)
                     /locus_tag="VEP73_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT26912.1"
                     /translation="MPRSLHPPLVAIALPRAAAAVAVVRAWEAGEAVLPLDPAAPDAE
                     TRRTLELASPTHLVDAAGRARLPGGRPVAPGVAAVVVTSGTAGAPKAVELTAAGIRAS
                     SLAVSASVGAGPGDRWLACVPLHGVAGLAIVARSWHTGVPAEVHDRFDPLLVDRAAAG
                     GATLVSVVPTMLGRLLDAGRVLGRFRRVLLGGSAAPAGLLAAAAGAGAAVVRTYGLTE
                     TFGGVAHDGRALPGVELSVAPDGEIGVRGEMVMRGYRGDARATAATLRGGVLRTGDLG
                     RLEPDGRLVVLGRRDDLIVTGGVNVHPVEVERVLSDHPAVADAGVRGLPDPEWGERVV
                     AFVVPADPARPPTLDELRGFARDRLAAAKAPRQLVLVERLPRTPSGKVRRRLLGQQDW
                     "
BASE COUNT          182 a          626 c          605 g          135 t
ORIGIN      
        1 cgatgtcgat gcggagctcg ccgccgtctg gcggggctac gaccatggcg tcgtcaccga
       61 ggaagaggtg caccagcgcc tcgacgagct cgaacggctg ctctggcgcc aggtgttcca
      121 gcggctgcgc cgcgtccagc cgcccgagcc cgcggcgacg gccggaggcg ctcccgagcg
      181 cgtcccggcg acctgatccg gcagtccccc acggcgaggg ggcggcccgc gcgcaccgcg
      241 ggccgccccc tcgccacgtc gtgtccccgt cccggcgcct cgcgccgcgg ccggggcggt
      301 cgctgctacc agtcctgctg gccgagcagg cggcggcgca ccttgcccga gggcgtgcgg
      361 ggcagccgct cgaccaggac gagctggcgg ggcgccttgg ccgcggccag acggtcgcgg
      421 gcgaaccctc gcagctcgtc gagggtcggc ggccgcgccg ggtcggcagg gacgacgaag
      481 gcgacgaccc gctcgcccca ctcggggtcg gggagccccc gcacgcccgc gtcggcgacg
      541 gccgggtggt ccgacaggac ccgctcgacc tcgaccgggt gcacgttcac cccgccggtg
      601 acgatgaggt cgtcccgccg cccgaggacc acgaggcggc cgtccggctc caggcggccg
      661 aggtcgccgg tgcggagcac gccgccgcgc agggtggcgg cggtcgcccg ggcgtcgccc
      721 cggtagccgc gcatcaccat ctcgccgcgc acgccgatct cgccgtcggg ggcgaccgac
      781 agctcgacgc cgggcagcgc gcggccgtcg tgggcgacac cgccgaacgt ctcggtgagc
      841 ccgtaggtgc gcacgaccgc cgcgccggca ccggcggcgg ccgcgagcag cccggccggt
      901 gcggccgacc cgccgagcag gaccctgcgg aagcggccga ggacgcggcc cgcgtccagg
      961 agccgcccga gcatcgtcgg gacgaccgag accagcgtgg cgccgcccgc tgcggcccgg
     1021 tcgaccagca gcgggtcgaa gcggtcgtgg acctcggccg gcacgccggt gtgccacgac
     1081 ctcgcgacga tcgcgagccc ggcgacgccg tgcagcggca cgcaggcgag ccagcggtcc
     1141 ccggggccgg cgccgaccga cgccgagacg gcgagcgacg acgcccggat gccggcggcg
     1201 gtcagctcga ccgccttggg cgccccggcc gtccccgacg tgaccacgac cgccgcgacg
     1261 cccggcgcga ccggccgccc accgggcagg cgggcccggc cggccgcgtc gaccaggtgg
     1321 gtggggctgg ccagctcgag cgtgcgcctg gtctcggcgt ccggcgcggc ggggtcgagc
     1381 ggcaggacgg cctcccccgc ctcccaggcg cgcacgacgg ccacagcggc ggccgcccgg
     1441 ggcagggcga tcgccaccag cggcgggtgg agggacctcg gcacggtggg taccctagca
     1501 gccgcacccc gaccccgtag ccccgacccc gtagcccgac cccgtacc
//
LOCUS       DASPYY010000672         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_110056, whole genome shotgun sequence.
ACCESSION   DASPYY010000672 DASPYY010000000
VERSION     DASPYY010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110056"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VEP73_10570"
     CDS             complement(<1..341)
                     /locus_tag="VEP73_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT26913.1"
                     /translation="MMDCPLTLRSLYERARTLFPDKQLVSRRASGEVVRTTYADWAER
                     VARLAAALRSLGLEAGDRVGTFCWNHDRHLEVYFAAALSGGSYHTLNIRLGPDQIAYI
                     LNHAGDKVLVV"
     gene            complement(458..979)
                     /locus_tag="VEP73_10575"
     CDS             complement(458..979)
                     /locus_tag="VEP73_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26914.1"
                     /translation="MEYGEGAYLSYVCRSRRCGKVRISMPRLDEAVTDLVLTKLERES
                     VRPIAEDDQDETRAEIETVKNRLIRVAEEFADDEDVDPEQVRAMSRRLRNRLKELYAR
                     QAERAWSDVLDVIEPSDLRRLWDSDELTLARKRDIIAKVTKDSLVVYPATRLGRTFDP
                     SRIDVPAWREGGL"
     gene            complement(1171..1971)
                     /locus_tag="VEP73_10580"
     CDS             complement(1171..1971)
                     /locus_tag="VEP73_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT26915.1"
                     /translation="MSEQPPPTSDPRKDLNGAGALVTGGASGLGAATARALAARGALV
                     AVVDRDGARAAGLVGELGGARWFAADVTDEAQLGAAVDGAAAGFGGLRVAVLCAGIGW
                     AERVVGRDGPAALAPFNTVIQVNLVGTYNALRLAAAAMAANEPDGDGQRGVVVMTASV
                     AAFDGQIGQTAYAASKGGVVGLTLPAARDLARVGVRVCTIAPGIFDTPLLGQLPEQVR
                     QSLGAGVPFPPRLGQPEEYALLACQIAENPYLNGEVIRLDGALRMPPK"
     gene            2152..2895
                     /locus_tag="VEP73_10585"
     CDS             2152..2895
                     /locus_tag="VEP73_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26916.1"
                     /translation="MTRYDYAPDPPAGTASPMGGGTDADQLKDQVADQVREKAQVAKD
                     RARAATWQARGRLREQVDQRSTQAGERVSGTAGDVRGVAEELRRQGKDATARLADQAA
                     DRADHVGRYLKAADADRLLADAEDFARKRPWAVAAGGLALGFAASRLLKASSSQRYRS
                     YQDGDRDRSWFDDDRSVATGPLAADVPPVVGTYDPDPTAAGPDPTAATHAPVDAYADL
                     RPRPEVGEGPDPEPPVDTRRRPADPEPLT"
BASE COUNT          449 a         1094 c         1115 g          392 t
ORIGIN      
        1 tcgaccacca gcaccttgtc gccagcgtgg ttcaggatgt aggcgatctg atcagggccg
       61 agccggatgt tcagcgtgtg atagctgcct ccagagagcg cggcggcgaa gtacacctcc
      121 aggtggcggt cgtggttcca gcagaaggtg ccaacccggt cgcctgcctc taggccaaga
      181 cttcgcagcg cggcggccag gcgcgcgacc cgctcggccc agtccgcgta ggttgtccgc
      241 acgacctcgc cagaggcgcg cctgctgacc aactgcttgt ccgggaacag ggtccgggcg
      301 cgctcgtaca ggctacgaag ggtgagcgga cagtccatca tcgcacggac ctccttgatg
      361 cggctcggtt cggctcgatc gggcgcgctg gttctcctgc cgagatccta cgagtgcagc
      421 taaagcaggt cgagggggag gacctccccg ataccggcta cagcccaccc tcccgccagg
      481 ccggcacgtc gattcggctc ggatcgaagg ttcggccgag acgagtcgcc gggtacacga
      541 cgagggaatc cttggttacc ttggctatga tgtcgcgctt gcgagcaagc gtcagctcat
      601 ccgagtccca gagtcggcgc aggtccgaag gctcgatgac atccagcaca tcagaccagg
      661 cacgttccgc ctgacgcgcg tacaactcct tcaggcggtt ccggagacgc cgtgacatgg
      721 cccgcacctg ttcggggtct acgtcttcgt cgtcagcgaa ctcctcggca acccggatga
      781 gccggttctt gaccgtctcg atctcggcgc gcgtctcgtc ttggtcgtcc tcggcgatgg
      841 ggcggacgga ctcgcgctcg agcttggtga gaacgaggtc cgtgaccgcc tcatcgaggc
      901 ggggcatcga gatgcggacc ttgccgcacc tccgactccg gcaaacgtag ctgagatatg
      961 ccccttcgcc gtattccatg gccgtcccgt caccgtggca acccctcgct tgtcgaggtc
     1021 gtggcatggt tccagcgacc ccggtgacgc gcttctcgtg gcttgagccc gcgctcatgg
     1081 gcacccggcg gtggccgagg cggcggtgat cgtgcacgag cgcgctacgt cggcggcctg
     1141 gtcaccgacg acgccgagga gcgctgacgc ctacttcggc ggcatccgca gggcgccgtc
     1201 cagccggatc acctcgccgt tgaggtacgg gttctcggcg atctggcagg cgagcagggc
     1261 atactcctcg ggctgtccga gccggggcgg gaacggcacc ccggcgccga ggctctggcg
     1321 cacctgctcg gggagctggc cgagcagcgg ggtgtcgaag atgcccggcg cgatggtgca
     1381 gacgcggacc ccgacccggg cgaggtcgcg cgcggcgggg agggtgagcc cgacgacgcc
     1441 gcccttggac gcggcgtagg cggtctggcc gatctggccg tcgaaggcgg ccaccgacgc
     1501 ggtcatcacc acgacgcccc gctggccgtc gccgtcgggc tcattcgcgg ccatcgcggc
     1561 cgccgccagc cggagcgcgt tgtaggtgcc gaccaggttg acctggatga ccgtgttgaa
     1621 cggcgccagg gcggccgggc cgtcgcggcc gacgacgcgc tcggcccagc cgatgccggc
     1681 gcacaggacg gcgacgcgca gcccgccgaa gccggccgcg gcaccgtcga ccgccgcgcc
     1741 gagctgggcc tcgtcggtga cgtcggcagc gaaccaccgg gcaccgccga gctcgccgac
     1801 gagcccggcg gcccgggcgc cgtcccggtc gacgacggcc accagggcac cccgggcggc
     1861 gagcgcgcgg gcggtggccg cgccgagccc ggaggctcca ccggtgacga gcgcgccggc
     1921 gccgttcagg tccttcctcg ggtcgctcgt gggtggcggc tgctcggaca tgcggctcct
     1981 tcgacgactc ggccgacgac tcggccgacg actcggcggg cgccggtcgc gcccatctcg
     2041 tcgcggagcc taccgcgcgc caggcctctt cgaggttttc aaggcggtcc gcgagatacg
     2101 caggggacat gcaggagggg cacgaccagt gaacggagaa gggagatccg catgacccgg
     2161 tacgactacg ccccggaccc gcccgcgggg acggcgagcc ccatgggcgg tgggacggac
     2221 gccgaccagc tcaaggatca ggtcgccgac caggtcaggg agaaggcgca ggtggccaag
     2281 gacagggcga gggcggccac ctggcaggcc aggggacgcc tccgcgagca ggtggaccag
     2341 cgctccaccc aggccggcga gcgcgtcagc gggacggccg gggacgtccg cggcgtggcc
     2401 gaggagctgc gccggcaggg caaggacgcg acggcccgcc tggccgacca ggccgccgac
     2461 cgggccgacc acgtcggcag gtacctgaag gccgccgacg ccgaccgcct gctggccgac
     2521 gccgaggact tcgcccgcaa gcggccgtgg gccgtggcgg ccgggggcct cgcgctcggg
     2581 ttcgccgcgt cgcggctcct gaaggcgtcc agcagccagc ggtaccggtc ataccaggac
     2641 ggcgaccgcg accgctcgtg gttcgacgac gaccggtccg tcgccaccgg accgctcgcg
     2701 gcggacgtgc cgccggtcgt cggcacctac gacccggacc ccaccgcggc cggcccggac
     2761 ccgaccgcgg ccacgcacgc gccggtggac gcctacgccg acctccggcc gcggccggag
     2821 gtcggcgagg ggccggaccc ggagccgccg gtggacacgc ggcgccgccc ggccgacccc
     2881 gagccgctga cgtgacggct ggccgggccc gcaccgggag gtactgatgg ccactcgcga
     2941 agagccgttc ccaccgggcg acctgcacga gcgcccgctg cccgagctgc tgaagcagct
     3001 ctccgaggag accacccagc tcctgcgcta ggagatcgag ctcgcaaagg
//
LOCUS       DASPYY010000673         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1376578, whole genome shotgun sequence.
ACCESSION   DASPYY010000673 DASPYY010000000
VERSION     DASPYY010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376578"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="folK"
                     /locus_tag="VEP73_10590"
     CDS             <1..430
                     /gene="folK"
                     /locus_tag="VEP73_10590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYT26917.1"
                     /translation="VRALDADRHTHVMAVSRVYETVPVGGPEQGPYLNAVAVLETDRG
                     PGDLLDLLLATEAGLGRVRDERWGPRTLDLDLLLYDGPPIDSPELTVPHPRARERAFV
                     LVPLVDADPFARFPDGETALDALARLGPVAGVVPVGDLEV"
     gene            445..1233
                     /locus_tag="VEP73_10595"
     CDS             445..1233
                     /locus_tag="VEP73_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT26918.1"
                     /translation="MTGRGRVRFELLMRQVRAALPPPPARVVDAGGGTGQLAVPLAGL
                     GYQVTVVDTSAAMLATCAQRAAAEGRGASARLATVQGDAADVAGLLGGASQDAVICHD
                     VLAGVDDPAELLGALAGVLREGGTLSLAFPNRDWLALRAGRRGDYAGALRLLDDPLEA
                     AEVSAAWRGARSESPGPGARRAYTAAELRPWLDKAGFELAGVLGVGVFAEGRDEELDR
                     AASAALLQLEQQVADREPYRSGAQTVHIVGRRRVLRHALAGYTT"
     gene            1260..>2149
                     /locus_tag="VEP73_10600"
     CDS             1260..>2149
                     /locus_tag="VEP73_10600"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HYT26919.1"
                     /translation="MPDALSAQLSPAAVIGAGRVGLALATFLRRIGAEVTIGVRSDRG
                     RERAEGLGLATAQAHEAAKPASLVLLTVQDDELPALITSLAAEGTFATGQLVVHTAGV
                     DGPALLAPAAAAGARIAACHPVQVFNDDLEATVGRLPGTVWGVTGDPAGRQLVLALGG
                     RPMDVPESGRVRYHAALVLAANGAAALAASASDILRAGGVIDPAAVLSSLVHASVDSA
                     LATGQAGMSGPWVRGDGRTVAMHTEALARKQKVVNAYVAVARLVADRAAAAGRLDPEA
                     HHRVDAALRGQSPDDHDGNE"
BASE COUNT          232 a          831 c          832 g          254 t
ORIGIN      
        1 ggtgcgggcg ctggacgccg accgccacac ccacgtgatg gcggtctccc gggtgtacga
       61 gacggtgccg gtgggcggac cggagcaggg tccctatctc aacgcggtgg ccgtgctgga
      121 gaccgaccgg gggcctgggg acctgctcga cctcctgctc gccaccgagg cgggactcgg
      181 ccgggtgcgg gacgagcgct gggggccgcg cacgctcgac ctcgacctgc tcctgtacga
      241 cgggccgccg atcgactcgc ccgagctgac ggtgccccac ccgcgggccc gcgagcgcgc
      301 gttcgtgctc gtgccgctgg tcgacgccga cccgttcgcc cgcttccccg acggcgagac
      361 ggcgctcgac gccctggccc gcctcggccc ggtcgcgggc gtcgtgcccg tcggcgacct
      421 ggaggtctag atgaggaggt ctggatgacc ggacgtggcc gggtacggtt cgagctgctg
      481 atgcgccagg tccgggccgc cctcccgccg ccgccggccc gggtcgtcga cgccgggggc
      541 ggtaccgggc agctcgcggt gccgctcgcg ggcctcggct accaggtcac cgtggtcgac
      601 accagcgcgg ccatgctggc cacctgcgcg cagcgggcgg cggccgaggg ccggggcgcg
      661 agcgccaggc tggccaccgt ccagggggac gcggccgacg tggcgggcct cctcggcggg
      721 gccagccagg acgccgtgat ctgccacgac gtgctcgccg gcgtcgacga cccggccgag
      781 ctgctcggcg ccctcgccgg cgtgctccgc gagggcggga cgctgtccct cgccttcccc
      841 aaccgggact ggctggccct gcgggcgggg cggcgtggcg actacgccgg cgcgctccgc
      901 ctgctcgacg acccgctcga ggcggccgag gtcagcgccg cctggcgggg ggcgaggagc
      961 gagtcgcccg ggccgggcgc gcggcgcgcc tacaccgcgg cggagctgcg cccctggctc
     1021 gacaaggccg gcttcgagct ggccggggtg ctcggcgtgg gtgtcttcgc cgaggggcgt
     1081 gacgaggagc tggaccgcgc cgcctccgcc gcgctgctcc agctcgagca gcaggtcgcc
     1141 gaccgggagc cgtaccggtc gggtgcgcag acggtgcaca tcgtcgggcg ccgccgggta
     1201 cttcgtcatg cattggctgg atatactacg tagtccctca cgctcggatc ggggaacccg
     1261 tgcctgacgc actgtccgca cagctctcgc ccgccgccgt catcggcgcc gggcgggtgg
     1321 gcctcgcgct cgccaccttc ctgcgccgga tcggcgccga ggtcaccatc ggggtccgct
     1381 ccgaccgggg ccgggagcgg gccgagggcc tcgggctggc caccgcccag gcccacgagg
     1441 ccgccaagcc ggcgagcctg gtgctgctca ccgtgcagga cgacgagctg cccgccctga
     1501 tcacctcgct cgccgccgag ggcaccttcg ccaccggcca gctggtggtg cacaccgccg
     1561 gcgtcgacgg gcccgcgctg ctcgcgccgg ccgccgccgc cggggcccgg atcgccgcct
     1621 gccacccggt ccaggtcttc aacgacgacc tcgaggcgac cgtcggccgg ctgcccggca
     1681 cggtctgggg cgtcaccgga gaccccgcgg gccgccagct ggtgctcgcc ctcggcggcc
     1741 ggccgatgga cgtgcccgag tcgggccgcg tccgctacca cgccgccctg gtcctcgccg
     1801 ccaacggcgc ggcggcgctc gcggccagcg cgagcgacat cctgcgggcg ggcggggtga
     1861 tcgacccggc ggccgtgctc tccagcctgg tccacgcgag cgtcgactcg gcgctcgcca
     1921 ccgggcaggc ggggatgtcg ggaccgtggg tgcgcggcga cggccggacc gtcgccatgc
     1981 acaccgaggc gctggcccgc aagcagaagg tcgtcaacgc ctacgtggcc gtcgcccggc
     2041 tggtcgccga ccgcgcggcc gcggccgggc gcctggaccc ggaggcccac caccgggtcg
     2101 acgccgccct gcggggccag agcccggacg accatgacgg gaacgagga
//
LOCUS       DASPYY010000674         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_814198, whole genome shotgun sequence.
ACCESSION   DASPYY010000674 DASPYY010000000
VERSION     DASPYY010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814198"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VEP73_10605"
     CDS             complement(<1..401)
                     /locus_tag="VEP73_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT26920.1"
                     /translation="MTRPTRAVFVAHAFPRSREDLPGHFLLGLATALGDEGVEVEAVA
                     PSAPGLAARERLDGVAVRRYRYAPRRAETLAYAGTMHVKAASPSGALALAGLLGAGAL
                     AVRRAAGRADLVHAHWWFPGGVQALASRRRPL"
     gene            complement(604..>2177)
                     /locus_tag="VEP73_10610"
     CDS             complement(604..>2177)
                     /locus_tag="VEP73_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26921.1"
                     /translation="LDAGGARQAVQSARARLGFPGTAFVLALAGGTGAGKSSLLNALA
                     GYEVSRPGPVRPVTDEPVAWVPADAAGELGPLLRWVGVERVVSHQDPSFAELCLLDLP
                     DYDSVERAHRARVDDVLPRVDAVCWVLDPEKYNDRVLHEDYLRPLAHHAERAVFVVNR
                     RDVIGGPAAVAELVADVRRTLAADGIHGRPVFAVSAAPPDGAGTGELDALRTWLSERM
                     QAKSVVADRVAADCEAAGAEIGRRAGLDGAGASRPLVDARVREAARERAVAAARAAVD
                     VAGVRRACERRTRAEARAAGAGPVGRALAGIARLRGGDRAGAASARSIDPLAYARGWR
                     RRSSLSRTVNPVHELLRRAATAAPPALRADVMALARPDRLEERLGDAVDEAVARAGVE
                     HARPPRSALWPLVGALQTLATVAFLAGALWYLTLYLAGRARADLPGLPTFHGVPAPLL
                     LVVGGLAAGWLFARVLHASARRQGRRWADRLTGDLDRAVAREVDQAMSAPLADLETAR
                     AELLVRLADLRQAAA"
BASE COUNT          246 a          885 c          841 g          205 t
ORIGIN      
        1 agcggccgcc gtcgcgaggc cagcgcctgg acgccgccgg gaaaccacca gtgggcgtgg
       61 accaggtcag cccgccccgc ggcccggcgc acggccaggg cgcccgcgcc gagcagcccg
      121 gcgagcgcca gggccccgga cgggctcgcc gccttgacgt gcatcgtccc cgcgtaggcg
      181 agcgtctcgg ctcgccgggg cgcgtagcgg tagcggcgca cggcaacccc gtccaggcgc
      241 tcgcgcgcgg ccaggccggg cgccgacggg gcgaccgcct ccacctcgac cccctcgtcg
      301 ccgagcgccg tggcgagccc gagcaggaag tgcccgggca ggtcctcgcg ggagcggggg
      361 aaggcgtggg ccacgaacac ggcccgggtc ggccgggtca tggcgcatgt tcgtacgccg
      421 ccgaggccgg cggcaagcgg ctccgttgcg gccggccggg atgcgcgggc gggtgatgtc
      481 catctacggc atcgtcttcc tcggctcgac gccgctcggc agcctcgtcg tcggcgggat
      541 ggccgagcgc tgcggaacgc tcccaccgag ccgcctgacc tggcgtccca tctcggcgca
      601 ggctcaggcg gcggcctggc gcaggtcggc gaggcggacg agcagctcgg ccctggccgt
      661 ctccaggtcg gcgagcgggg cgctcatcgc ctggtccacc tcgcgggcga cggcccggtc
      721 gaggtcaccg gtgagccggt cggcccaccg ccggccctgc cggcgggcgc tcgcgtgcag
      781 cacgcgggcg aagagccagc cggcggcgag gccgccgacg accaggagga gcggcgccgg
      841 gacgccgtgg aacgtcggca ggccgggcag gtcggcgcgt gcccgcccgg ccaggtacag
      901 ggtcaggtac cacagggcgc cggcgaggaa cgccacggtc gcgagcgtct gcagcgcgcc
      961 gacgagcggc cacagcgccg agcgcggcgg ccgggcgtgc tccaccccgg cccgggccac
     1021 cgcctcgtcg accgcgtcgc cgaggcgctc ctccaggcgg tcgggcctgg ccagcgccat
     1081 cacgtcggcc cgcagcgcgg ggggcgccgc cgtcgccgcc cggcgcagca gctcgtggac
     1141 cgggttgacg gtccggctga gcgaggagcg cctccgccag ccgcgggcgt aggccagcgg
     1201 gtcgatggac cgggccgacg ccgcgcccgc ccggtcgccg ccccgcagcc gcgcgatgcc
     1261 cgccagcgcc cgccccaccg ggccggcgcc ggcggcgcgc gcctcggccc tggtgcggcg
     1321 ctcgcaggcg cggcgcaccc ccgcgacgtc gaccgcggcc cgggccgccg ccaccgcccg
     1381 ctcgcgcgcc gcctcgcgca ctcgagcgtc gaccagcggc cggctcgcgc ccgcgccgtc
     1441 caggcccgcg cgccggccga tctccgcgcc ggccgcctcg cagtcggccg ccacccggtc
     1501 ggcaacgacc gacttggcct gcatgcgctc ggacagccag gtccgcaggg cgtccagctc
     1561 accggtgccg gcaccgtcgg gtggcgcggc cgagaccgcg aacaccggcc gcccgtggat
     1621 gccgtcggcg gccagggtgc gccggacgtc cgcgaccagc tcggcgacgg cggcgggccc
     1681 cccgatcacg tctcgccggt tgaccacgaa gacggcccgc tcggcgtggt gcgcgagcgg
     1741 gcgcaggtag tcctcgtgca gcacgcggtc gttgtacttc tcggggtcga ggacccagca
     1801 gaccgcgtcc acccggggga gcacgtcgtc gacccgtgcc cggtgggcgc gctcgaccga
     1861 gtcgtagtcg ggcaggtcga gcaggcagag ctcggcgaac gacgggtcct ggtggctcac
     1921 cacccgctcg acgcccaccc agcgcagcag cggcccgagc tcgcccgcgg cgtcggccgg
     1981 cacccacgcg accggctcgt cggtcacggg gcggaccggg cccggcctgc tcacctcgta
     2041 ccccgcgagc gcgttgagca ggctcgactt gcccgccccc gtccccccgg ccagagcgag
     2101 cacgaaggcg gtgcccggga accccagccg ggcgcgtgcc gactgcaccg cctgccgcgc
     2161 gccccccgcg tcgagcc
//
LOCUS       DASPYY010000675         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1657124, whole genome shotgun sequence.
ACCESSION   DASPYY010000675 DASPYY010000000
VERSION     DASPYY010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657124"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..837)
                     /locus_tag="VEP73_10615"
     CDS             complement(166..837)
                     /locus_tag="VEP73_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HYT26922.1"
                     /translation="MSRVTERRPAVPLAPTRPAATPPGGGYLEGTLGRFLDQVASGEP
                     APGGGAAAAVTVALAAGLVAMAGRFSAGRMDARDDAEPGEVVDLVARAEELRARVAPL
                     AQADADAYGRVLAAMRLPREPDPAARRLAVRSALSAAADVPLAVAEVAAETADLAAVL
                     AEAGNPNLRGDAVTAGLLAEAGAWSAACLVEINVGAGDERAVRAGNLAHAAAGAARRA
                     MDARR"
     gene            complement(843..>2534)
                     /locus_tag="VEP73_10620"
     CDS             complement(843..>2534)
                     /locus_tag="VEP73_10620"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HYT26923.1"
                     /translation="MPSALEISRQATMKPIAGIAEEIGIPPWLIRPYGEHVAKIDLAA
                     IDALADRPRAKYVVVTAVTPTPLGEGKTTTTVGLGQAFGHIGRQATVAIRQPSMGPTF
                     GIKGGAAGGGYSQVVPFETLNLHLTGDMHAVTAAHNMLSAILDNHLHQGNKLGLDVNE
                     ITWRRVLDVNDRALRNLVVGLGSKQDGVPRQSGFDITAASEVMAVLALSTSLQDMRRR
                     LGRVVVGYNRHGEPVTAEQLKAAGAMTVMMREAIKPNLMQTMENTPVLVHAGPFGNIA
                     HGNSSVVADLIGIHGGDFLVTEAGFGADMGAERFFNIKCRTSGLRPDAAVVVATIRAL
                     KAHSGRHRVVAGKPLPPALLEENPDEVHLGGVNLVKQIENIRLHGVPPVVAVNAFPGD
                     HPSEHAAVREIAESMGARAAVCTHFAKGGAGAVELAEAVAEAAEEPNDFRFLYPDSAS
                     LREKIEVVATKVYGASEVEYDLVAARQLETYERNGYGHLPVCIAKTHLSISSDPTLKG
                     APTGWVLPVREVRASVGAGFIYPICGDMRTMPGLALHPAAERIDIDEHGEIVGLS"
BASE COUNT          308 a          915 c          956 g          355 t
ORIGIN      
        1 cgcgccgttc gtacgcgtgc gaggcgtagt cgtcgccgac cagcatggtg gcgagcccgg
       61 ccttgacccc ctgctcggcg agccccttca tctcgacctg gaggcgcccc ttgagctcgt
      121 cggccagcgc gttgccgtcg atcacggtcg cagccatccg cgcccctacc gccgggcgtc
      181 catggcccgg cgggccgcac ccgcggccgc gtgggccagg ttcccggccc gcacggcgcg
      241 ctcgtccccg gcgcccacgt tgatctccac caggcaggcc gccgaccagg cgcccgcctc
      301 ggcgagcagc ccggcggtga ccgcgtcgcc gcggaggttg gggttgccgg cctcggccag
      361 cacggcggcc aggtcggcgg tctcggcggc gacctcggcg acggccagcg gcacgtcggc
      421 ggccgccgac agcgccgacc ggaccgcgag gcggcgggcc gccgggtccg gctcgcgggg
      481 caggcgcatc gcggcgagga cccgcccgta ggcgtcggcg tcggcctgtg ccaggggcgc
      541 cacgcgggcg cgcagctcct ccgccctggc caccaggtcg acgacctcac cgggctcggc
      601 gtcgtcgcgg gcgtccatgc ggccggcgga gaaccggccc gccatcgcca ccaggccggc
      661 ggccagcgcc acggtcaccg cggcggccgc gccgccgcct ggcgccggct cgcccgacgc
      721 cacctggtcg aggaagcggc cgagcgtgcc ctcgaggtag ccgccgcccg gcggcgtcgc
      781 ggccgggcgt gtcggcgcga gtggcacggc gggccgccgc tcggtcaccc ggctcaccgg
      841 ggtcacgaca gccccacgat ctcgccgtgc tcgtcgatgt cgatgcgctc ggcggccggg
      901 tgcagcgcga ggccgggcat cgtccgcatg tcgccgcaga tcgggtagat gaagccggcg
      961 ccgaccgacg cccggacctc gcggaccggc agcacccagc cggtcggggc gcccttgagc
     1021 gtcgggtcgg acgagatcga caggtgcgtc ttggcgatgc agaccggcag gtgcccgtag
     1081 ccgttgcgct cgtaggtctc gagctggcgg gcggcgacca ggtcgtactc gacctcgctg
     1141 gcgccgtaca ccttcgtcgc gaccacctcg atcttctcgc ggagcgacgc ggagtcgggg
     1201 tagaggaagc ggaagtcgtt cggctcctcg gccgcctcgg cgaccgcctc ggcgagctcg
     1261 accgccccgg ccccgccctt ggcgaagtgg gtgcagaccg cggcccgggc ccccatcgac
     1321 tcggcgatct cgcgcacggc ggcgtgctcg gacgggtggt cgccggggaa ggcgttgacc
     1381 gcgacgaccg gcgggacgcc gtggagccgg atgttctcga tctgcttgac gaggttgacg
     1441 ccgccgaggt ggacctcgtc ggggttctcc tcgagcagcg ccggcggcag cggcttgccg
     1501 gccacgaccc ggtggcgccc ggagtgcgcc ttgagcgcac ggatggtggc gacgaccacc
     1561 gcggcgtccg ggcgcaggcc cgacgtgcgg cacttgatgt tgaagaaccg ctcggcaccc
     1621 atgtcggcgc cgaacccggc ctcggtgacc aggaagtccc cgccgtggat gccgatcagg
     1681 tcggcgacga ccgacgagtt gccgtgggcg atgttgccga acgggcccgc gtgcacgagc
     1741 accggggtgt tctccatggt ctgcatgagg ttcggcttga tcgcctcgcg catcatgacg
     1801 gtcatcgcgc ccgccgcctt gagctgctcg gcggtgaccg gctcgccgtg gcggttgtag
     1861 ccgacgacga cccggccgag ccggcggcgc atgtcctgca gcgacgtgga cagggcgagg
     1921 acggccatga cctcggaggc ggccgtgatg tcgaagccgc tctggcgggg gacgccgtcc
     1981 tgcttggagc cgaggccgac cacgaggttg cgcagcgccc ggtcgttgac gtcgagcacc
     2041 cggcgccagg tgatctcgtt gacatcgagg ccgagcttgt tgccctggtg cagatggttg
     2101 tcgagtatcg cggagagcat gttgtgcgcg gcggtgaccg cgtgcatgtc gccggtcagg
     2161 tgcaggttca gcgtctcgaa cggcacgacc tggctgtagc cgccaccggc cgcgccaccc
     2221 ttgatgccga aggtcgggcc catcgacggc tgcctgatgg cgaccgtggc ctgtcttccg
     2281 atgtggccga acgcctggcc gaggccgacg gtcgtggtgg tcttgccctc gccgagcggg
     2341 gtcggcgtga cagccgtcac gaccacgtac ttggcgcgag gccggtcggc gagcgcgtcg
     2401 atggcggcga gatcgatctt cgccacatgc tcgccgtagg gccggatcag ccatggcgga
     2461 atgccgatct cctccgcgat gccggcgatc ggcttcatgg tcgcctgccg ggagatctcc
     2521 agtgcgctag gcat
//
LOCUS       DASPYY010000676         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_954757, whole genome shotgun sequence.
ACCESSION   DASPYY010000676 DASPYY010000000
VERSION     DASPYY010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954757"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VEP73_10625"
     CDS             <1..660
                     /locus_tag="VEP73_10625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT26924.1"
                     /translation="NLAKRTVAPDLELVYEAGVFGARPARLPLSIGDPTIVTGATAIT
                     SMYELFSFYLQGGLIDVGFLGAAQLDRYGNINTTVIGAYDRPKVRLPGSGGACEIAIN
                     AREVFVLMRQSARSFVERIDFRTSPGNLGGAALRAAQGWQGRGPSLVATDLGTYRFDA
                     GSGEMVLVSVHPGVTVDDVRAATGWDVRVADDLGTTPAPTGEELRLIREELDPGRIYV
                     R"
     gene            650..1969
                     /locus_tag="VEP73_10630"
     CDS             650..1969
                     /locus_tag="VEP73_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT26925.1"
                     /translation="MSGSHVFSRAPGAGLPRVARAQGARVWDADGRCYLDGAGGAIVV
                     GVGHGDQRVVEAIRAQALRVAYAHGTMFTSDALEAYASELAAVVPVDGARVYPVSGGS
                     EAIETALKMARAYHLARGEDRHLVIGRHGSYHGNTRGALDAGGREPLRRPYLPWLGQA
                     RHTLAAYEYRCPFPATHPDGCGRRYADALEAAIVAAGPGTVAAFVAEPVAGAALGAAV
                     PPDDYWPAVADVCRRHGVLLVADEVMTGFGRTGRWFGCDHWDVRPDILAAAKGAASGY
                     WPLGLAVASGPVHDTIAGAGFTHGLTFSHHVVGAAAGREVLRILRSDRLVEASAAQGK
                     RLLAELEGRLAGHPAAGDVRGLGLMVAVELVADRAGRTPFPRSARVAERVVAAARDRG
                     LLLYGSTGCADGTRGDLVMLGPPLVVTDAEVDEMAATPAAAIAAATG"
     gene            complement(2016..>2375)
                     /locus_tag="VEP73_10635"
     CDS             complement(2016..>2375)
                     /locus_tag="VEP73_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYT26926.1"
                     /translation="ALTVALAAALCLLTGLARLAWRVWRSWRAPSILGAEALVGRRAT
                     VRRATGRAGQVLLDGVLWRARSSGGPLRAHQTVEVLGVEGLELVVAPEERHGREDWEE
                     WDPGAGGGVTALHARWG"
BASE COUNT          263 a          943 c          896 g          273 t
ORIGIN      
        1 aacctcgcga aacggaccgt cgcgcccgat ctggagctcg tctacgaggc cggcgtcttc
       61 ggcgcccggc cggctcgcct cccactctcg atcggcgacc cgacgatcgt gaccggcgcc
      121 acggcgatca cgagcatgta cgagctcttc agcttctatc tccagggcgg cctgatcgac
      181 gtcggcttcc tcggcgcggc ccagctggac cggtacggca acatcaacac caccgtgatc
      241 ggcgcctacg accgcccgaa ggtgcgcctg cccggctcgg gaggcgcgtg cgagatcgcg
      301 atcaacgcgc gcgaggtgtt cgtgctgatg cgccagtcgg cccgctcgtt cgtcgagcgg
      361 atcgacttcc gcacgagccc gggcaacctc ggcggcgccg ccctgcgggc cgcccagggc
      421 tggcagggcc ggggaccgtc gctggtcgcc accgacctcg gcacctaccg gttcgacgcc
      481 ggcagcggcg agatggtgct cgtttccgtc cacccggggg tcacggtcga cgacgtgcgg
      541 gcggcgaccg gctgggacgt gcgggtcgcc gacgacctcg ggaccacgcc ggccccgacc
      601 ggcgaggagc tgcgcctcat ccgggaggag ctcgacccgg ggcggatcta tgtcaggtag
      661 ccacgtcttc tcccgcgcgc cgggcgccgg gctgccgcgg gtcgcccggg cccagggcgc
      721 gcgggtgtgg gacgccgacg gccgctgcta cctcgacggg gccggcggcg cgatcgtcgt
      781 cggcgtcggc cacggcgacc agcgggtcgt ggaggcgatc cgcgcccagg cgctgcgggt
      841 cgcctacgcg cacggcacga tgttcacctc cgacgcgctc gaggcctacg cgagcgagct
      901 ggccgcggtg gtcccggtcg acggcgcccg cgtctacccg gtgtcgggcg gcagcgaggc
      961 gatcgagacc gcgctcaaga tggcgcgcgc gtaccacctc gcccgcgggg aggaccgcca
     1021 cctggtgatc ggcaggcacg ggtcctacca cggcaacacc cgcggcgcgc tcgacgccgg
     1081 cggccgcgag ccgctgcgcc ggccctacct gccgtggctc gggcaggcgc gccacacgct
     1141 cgccgcctac gagtaccgct gcccgttccc ggcgacgcac ccggacggtt gcgggcggcg
     1201 ctacgcggac gcgctcgagg cggccatcgt cgcggccggg ccggggacgg tcgccgcgtt
     1261 cgtcgccgag ccggtcgccg gggcggcgct cggcgcggcc gtgccgcccg acgactactg
     1321 gccggcggtc gcggacgtgt gccggcgcca cggcgtgctg ctcgtcgcgg acgaggtgat
     1381 gaccggcttc ggccgcaccg ggcgctggtt cggctgcgac cactgggacg tccggcccga
     1441 catcctcgcc gcggccaagg gtgccgcctc cggctactgg ccgctcgggc tcgcggtggc
     1501 gagcggaccg gtccacgaca cgatcgcggg ggccgggttc acccacgggc tcaccttctc
     1561 ccaccacgtc gtgggggccg ccgccggccg cgaggtgctg cgcatcctcc gctccgaccg
     1621 gctcgtcgag gcgtccgcgg cgcagggcaa gcggctgctc gccgagctcg agggccggct
     1681 cgccgggcac ccggccgccg gggacgtgcg cggcctcggc ctgatggtcg cggtcgagct
     1741 ggtcgccgac cgggccggcc ggacgccgtt cccgcgctcg gcgcgggtcg ccgagcgggt
     1801 cgtcgccgcc gcccgcgacc gcggcctgct cctgtacggc tcgaccggct gcgccgacgg
     1861 cacgcggggc gacctcgtca tgctcggccc gccgctggtc gtcaccgacg cggaggtcga
     1921 cgagatggcg gcgacgcccg ccgccgcgat cgcggcggcc acggggtgac cgcccagggc
     1981 gggccggacc gggcccccgg tccggcccgc ccgggctacc cccagcgggc gtgcagggcg
     2041 gtcaccccgc cgccggcgcc cggatcccac tcctcccagt cctcccgccc gtgccgttcc
     2101 tccggcgcga cgacgagctc gagcccctcc acgccgagga cctcgacggt ctggtgcgcc
     2161 cgcagcggcc cgccgctcga gcgcgcccgc cacaggacgc cgtccagcag cacctggcca
     2221 gcccggccgg tggcgcgccg cacggtggcg cgccggccga ccagcgcctc ggcgccgagg
     2281 atgctcgggg cgcgccacga ccgccaaacc cgccaggcga gccgcgcgag ccccgtgagc
     2341 aggcagagcg cggcggccag cgcgaccgtg agcgc
//
LOCUS       DASPYY010000677         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1939928, whole genome shotgun sequence.
ACCESSION   DASPYY010000677 DASPYY010000000
VERSION     DASPYY010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939928"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1542
                     /locus_tag="VEP73_10640"
     CDS             <1..>1542
                     /locus_tag="VEP73_10640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYT26927.1"
                     /translation="ISFAVAVAVLGGAGLSASFPLQRPATAGTVRDVPLSFASGSVAG
                     RLATRAPRALRGAPGRPGAWTSDAVRAGAARLVGLSWARTSDGPWTRRGGAETWLRTE
                     AASGRWSAWQPVQPADDGPDPGSPEEHPDHVFSDGVWLPAGTRAVQIKVDLPGSAPAA
                     VDGLTAHLVAPDMTPTPGTEAGRGGAIASTLQPPIVTRARWGADERIRRAQPRYATAV
                     RAAFVHHTAQANGYSRAESAALVRADYVYHVKTRGWNDIGYNFLVDRYGQVFEGRYGG
                     VDRAVVGACTAGFNTATTCVALLGTFTTASPPAPMVASLTNLLAWKLDLDHVDPLGAT
                     VLTSSGGATARYGNGIRVRLRTVSGHRDTSYTACPGNAAYALLPSLRAAVARTGLPKI
                     YGGWPSAGTVDPAARSTVSLAPRFTQAMSWSAWATDSGGRVVRRWSGTGTSARILWNG
                     RDAAGGVAPAGRAVLTVTARAGSVPARPLTVGVVVKLPRLPTSPLPDGTLVGGSPVRR
                     VRGGAL"
BASE COUNT          169 a          641 c          550 g          182 t
ORIGIN      
        1 catctccttc gccgtcgccg tcgccgtcct cggcggcgcc ggcctctccg cctcgtttcc
       61 cctccagcgc ccggccaccg ccgggacggt cagggacgtg cccttgtcgt tcgcgtccgg
      121 ctccgtggcg ggacggctgg ccacccgcgc cccgcgggcg ctgcgaggcg cccccgggcg
      181 gcccggggcc tggacgtcgg acgcggtccg cgccggagcg gcccgcctgg tcgggctgtc
      241 gtgggcccgg acctcagacg gcccctggac gcggcgcggc ggcgccgaga cctggctgcg
      301 gaccgaggcc gcctctggcc gctggtccgc ctggcagccg gtgcagcccg ccgacgacgg
      361 gcccgacccc ggcagtcccg aggagcatcc ggaccacgtc ttcagcgacg gggtctggct
      421 ccccgccggc acccgtgcgg tgcagatcaa ggtcgacctg ccgggcagcg cccccgcggc
      481 cgtggacggg ctcaccgccc acctcgtcgc ccccgacatg accccgaccc ccgggaccga
      541 ggccgggcgg ggtggggcga tcgcctcgac gttgcagccg ccgatcgtca cgcgcgcccg
      601 ctggggcgcc gacgagcgca tccgccgtgc ccagccacgc tacgcgaccg cggtgagggc
      661 cgccttcgtg caccacaccg cccaggccaa cgggtactcg cgagcggaga gcgccgccct
      721 cgtccgcgcc gactacgtct accacgtcaa gacgcggggc tggaacgaca tcggctacaa
      781 cttcctcgtc gaccggtacg gccaggtgtt cgagggccgc tacggcggcg tcgaccgggc
      841 cgtcgtcgga gcctgcacgg ccggcttcaa caccgcgacg acctgcgtcg cgctgctcgg
      901 caccttcacg accgcgagcc ccccggcgcc catggtcgcc tcgctcacga acctgctggc
      961 ctggaagctc gacctcgatc acgtcgaccc gctcggcgcc accgtgctga ccagctccgg
     1021 cggcgccacc gcgaggtacg gcaacggcat ccgggtccgg ctgcgcacgg tcagcggcca
     1081 ccgcgacacg tcgtacacgg cctgcccggg caacgccgcc tacgcgctgc tgccgagcct
     1141 ccgcgccgcc gtcgcgcgga ccggccttcc caagatctat ggcggctggc cgagcgcggg
     1201 cacggtggac ccggcggcca ggtccaccgt cagcctggcg cccaggttca cccaggcgat
     1261 gagctggagc gcctgggcca cggactccgg cggccgcgtc gtgcggcgct ggtccgggac
     1321 gggcacctcc gcgcgcatcc tctggaacgg ccgcgacgcc gccggcggcg tcgcgccggc
     1381 cggccgggcg gtgctcaccg tgaccgcgag ggcgggcagc gtcccggccc ggcccctcac
     1441 cgtcggcgtg gtcgtcaagc tcccacgcct gcccacctcg ccgctgcccg acgggaccct
     1501 ggtcggcggc tccccggtgc ggcgggtccg cgggggcgcc ct
//
LOCUS       DASPYY010000678         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_391520, whole genome shotgun sequence.
ACCESSION   DASPYY010000678 DASPYY010000000
VERSION     DASPYY010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391520"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VEP73_10645"
     CDS             complement(<1..201)
                     /locus_tag="VEP73_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYT26928.1"
                     /translation="MKVPLYEYRCTDCDHRFEALVPAGRADLTGCPSCGAEQARRLLS
                     VFSAPRAASGVGAAAAPSGGCCG"
     gene            complement(373..1818)
                     /locus_tag="VEP73_10650"
     CDS             complement(373..1818)
                     /locus_tag="VEP73_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYT26929.1"
                     /translation="MDLDDPCFLPAYELLARYRARELSPVEVTRAALERIERLDPLVN
                     AFVTVTPELALDQARRAEAAWLAGEAAAPLAGVPASIKDLFPTKGIRTTRGSVRWRDW
                     VPDFDAPSVGRMRAAGVVLLGKTNTPEAGWKGSTSNRVAGITRNPWDLGRTPGGSSGG
                     AAVAVATGMGSLGLGSDGGGSVRIPAAFTGVYGLKPSHALVPGSGTGPFEHLGHVGPI
                     ARTVRDAALLLTVLAGPDERDRLSYDTPRDYLSALRAGVAGLRVAWCPSLVAGLDAEP
                     AVRDTSAEGVRELERLGAVVEEIPPPLGTEIVEAHRTLLSTGLAALYPDLEEVEAEIE
                     PDLAATVRSGRGYSGLEVVVAQVRQLQVYDAIRVLFERCDLLATPAVSVTAFQADRDF
                     PEQVNGRPGVRVSWTPYSFPFNHSGHPAASVPCGTAPDGLPVGLQLVGGWRRDDLVLR
                     ASAALEQARPWTGPLLRVTRGLLASPGATSR"
     gene            complement(1868..3064)
                     /locus_tag="VEP73_10655"
     CDS             complement(1868..3064)
                     /locus_tag="VEP73_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT26930.1"
                     /translation="MAGGRKAAREPARRATPPAKAAGGPRPRASLTARGVGMVLGGLV
                     LGIGAGIAAERALVGHDRKRDDPEAGEPFGELRGAPATVTSHDGTELHVEDFGSGPCL
                     VFSHGFSLTQDAWHYQRRDLPAEFRCVFFDQRGHGRSGRPANHDFSLDAFAGDLRAVL
                     DWTGEDRVVVVAHSMGGIAALKLSERWPEEVGTRVAGLVLVDSTYADALKGMSAAVTA
                     RGALTVQRAAIKTAFKFFGADPVRANQLRRRGSDLGYLGTRLFGFGSNPSASQVAFVD
                     RILAGTDVEVWAKVFPSLIDFDMSEALERLDVPTLVAVGDKDRLTPVASARHMAEKIG
                     GARLLILEDAGHNAFLEEHEVLDAEIAAFATEVLGPARRRRRRSARIPKLTGDGRISV
                     RRVRGS"
     gene            complement(3064..>3890)
                     /gene="alr"
                     /locus_tag="VEP73_10660"
     CDS             complement(3064..>3890)
                     /gene="alr"
                     /locus_tag="VEP73_10660"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYT26931.1"
                     /translation="RPVAAHLKVDTGMHRQGCAPADLPGLAAFALAEPGLDVEGLWSH
                     FAVADEPERRATTDAQLAAYRDALAAAAAAGLTPRWRHLANSAGATLRDDARFDLVRL
                     GIELYGLAPSPDLPATTARLRPALALRAAVSALRTVEAGERVSYGHRWAAPARTRVAT
                     LPVGYADGVRRGLSGRLRVRIGGRDHAQVGRVTMDQLMVDVGAVPVEVGDVATLLGDP
                     ARGEPGVRDWSVALDTIDYEVTCGLSCRLPRVHHHGDRGDRPARSGQVPAREGARV"
BASE COUNT          507 a         1478 c         1454 g          451 t
ORIGIN      
        1 gccgcagcag ccccctgacg gggcggcggc cgcgccgacg cccgaggccg cccgcggtgc
       61 cgagaagacg ctgagcagcc ggcgggcctg ctcggcgccg cacgacgggc agccggtcag
      121 gtcggcccgg ccggcgggca cgagggcctc gaagcggtgg tcgcagtccg tgcaacgata
      181 ctcgtacagc ggcaccttca cgtcctcccg agctggtccg acgatgccgg atggcgaggg
      241 gccatgacct cctcagcaac gaaggctacc aggtcggccc cacggtcacg tccccgcggc
      301 cgtctacctg gggcgtgacc accatccgcc cccccgagac agccaggtcc tctgtgcgac
      361 cgcccggtgc ggtcatcggg acgtcgcgcc gggagaagcg agcagccccc gcgtcacgcg
      421 cagcagcggc ccggtccacg gccgggcctg ctccagtgcg gcgctggctc gcagcacgag
      481 gtcatcccga cgccacccgc cgaccagctg gaggccgacc ggcaggccgt ccggtgcggt
      541 gccgcacggc acgctcgcgg ccgggtggcc gctgtggttg aaggggaagc tgtacggagt
      601 ccaggagacc cgcaccccgg ggcggccgtt gacctgctca gggaagtccc ggtcggcctg
      661 gaaggccgtg acggagacgg cgggcgtggc cagcaggtca cagcgctcga acaggacccg
      721 tatcgcgtcg tagacctgaa gctgccggac ctgggcgacg accacctcga ggccgctgta
      781 gccccggccc gacctgaccg tcgcggccag gtcgggctcg atctccgcct ccacctcctc
      841 gaggtccggg tagagcgcgg ccagccccgt gctgagcagc gtcctgtgcg cctccacgat
      901 ctcggtcccc agcggtggcg ggatctcctc gacgaccgcg ccgagtcgct ccagctcccg
      961 tacgccctcg gcggaggtgt cgcgcaccgc gggctcggca tcgagcccag ccaccaggga
     1021 aggacaccag gcgacccgca ggcccgcgac gccggcccgc agggcggaca ggtagtccct
     1081 tggcgtgtcg taggagagcc ggtcgcgctc gtccgggccg gccaggaccg tgagcagcaa
     1141 cgcggcgtcc cggaccgtcc gagcgatcgg gccgacatgg cccagatgct cgaacgggcc
     1201 tgtccctgag cccggcacca gcgcatggga cggcttgagc ccgtacacgc ccgtgaaggc
     1261 ggccgggatc cggaccgagc cgccgccgtc acttcccagc ccgagagagc ccatgccggt
     1321 ggcgacggcg accgcggcgc cgccgctcga cccgccgggc gtccgcccca gatcccacgg
     1381 gttccgcgtg atgccggcga cccggttgct ggtcgacccc ttccagcccg cctctggcgt
     1441 gttggtcttg ccgagcagca cgacgcccgc ggcgcgcatg cgccccaccg agggcgcgtc
     1501 gaagtccggc acccagtcac gccaccgcac cgagccccga gtggtccgga tccccttcgt
     1561 cgggaacagg tccttgatgc tcgcgggcac gcccgcgagc ggtgcggcgg cctcgcccgc
     1621 gagccacgcc gcctccgcgc gccgcgcctg gtccagcgcc agctcgggcg tgacggtcac
     1681 gaaggcgttc acgagcgggt ccagccgctc gatccgctcg agcgcggcac gggtcacctc
     1741 gaccggggag agctccctgg cgcggtaacg ggcgagcagc tcgtacgcgg gcaggaagca
     1801 cgggtcgtcc aggtccatca ggcctcctcg ccgcgccgcc ccggtcgtcg cgccgcccgg
     1861 gagcccgtta cgagccgcgc acgcggcgga cggagatccg gccgtcgccg gtgagcttgg
     1921 ggatccgggc ggagcggcgg cggcggcgcc tcgccgggcc gagcacctcg gtcgcgaagg
     1981 cggcgatctc ggcgtccagg acctcgtgtt cctcgagaaa ggcattgtgg ccggcgtcct
     2041 cgaggatcag caggcgggca ccgccgatct tctcggccat gtggcgggcg gaggccaccg
     2101 gggtgagccg gtccttgtcg cccacggcga cgagggtggg cacgtcgagg cgctcgaggg
     2161 cctcggacat gtcgaagtcg atcaggctgg ggaagacctt ggcccagacc tcgacgtcgg
     2221 tgcctgccag gatccggtcg acgaacgcga cctggctcgc cgaggggttg gaaccgaacc
     2281 cgaacaggcg cgtaccgagg tagccgaggt cgctgccgcg gcgccggagc tggttcgccc
     2341 ggaccgggtc ggccccgaag aacttgaacg ccgtcttgat ggcggcccgc tgcaccgtga
     2401 gcgccccgcg cgcggtgacg gccgcgctca tccccttgag ggcgtcggcg taggtcgagt
     2461 cgaccaggac caggccggcc accctggtcc ccacctcctc gggccaccgc tcgctgagct
     2521 tcagggcggc tatgccgccc atcgagtggg cgaccaccac gacccggtcc tcgccggtcc
     2581 agtcgagcac cgcccggagg tcgccggcga aggcgtccag ggagaagtcg tggttcgccg
     2641 gccggcccga gcgcccgtgg ccgcgctggt cgaagaacac gcagcggaac tcggcgggca
     2701 ggtcgcgccg ctggtagtgc caggcgtcct gggtcagcga gaagccgtgc gagaacacca
     2761 ggcacggtcc cgagccgaag tcctccacgt gcagctcggt cccgtcgtgg ctcgtgacgg
     2821 tggccggcgc gccgcgcagc tcgccgaacg gctcgccggc ctctgggtcg tcgcgcttgc
     2881 ggtcgtggcc gaccagggcg cgctcggccg cgatgccggc gccgatcccg aggaccaggc
     2941 caccgagcac catcccgacg ccgcgagcgg tcagggacgc cctcggccgc gggccgccgg
     3001 ccgccttggc gggcggggtg gcccggcggg cgggctcgcg ggcggccttc ctgccaccgg
     3061 ccactacacg cgcgctccct cgcgagccgg gacctggccg gaccgcgccg gccggtcgcc
     3121 tcggtcgccg tgatggtgca cgcggggcag ccggcaggac agcccgcacg tcacctcgta
     3181 gtcgatcgtg tccagggcca cggaccagtc ccgcacacca ggctcgccgc gcgcggggtc
     3241 gccgaggagg gtggccacgt cgccgacctc cacgggcacc gcgcccacgt cgaccatcag
     3301 ctgatccatc gtcaccctgc ccacctgggc gtggtcccgc ccgccgatcc gtacccggag
     3361 gcggccggac agcccgcgcc gcacgccgtc ggcgtacccc accgggaggg tggccacgcg
     3421 cgtccgtgcc ggcgcggccc agcggtgccc gtaggagacc cgctcgccgg cctccaccgt
     3481 gcgcagggcc gagaccgccg cccgcagcgc gagggcgggc cgcagccgcg ccgtcgtggc
     3541 gggcaggtcg gggctcgggg cgagcccgta gagctcgatc ccgagccgca ccaggtcgaa
     3601 gcgggcgtcg tcgcggaggg tggcaccggc gctgttggcc aggtggcgcc agcggggggt
     3661 gagcccggcc gccgcggcgg ccgccagcgc gtcgcggtag gcggcgagct gggcgtcggt
     3721 ggtcgcccgc cgctctggct cgtcggccac agcgaaatgg ctccagagcc cctccacgtc
     3781 gagccctggc tcggccaggg cgaaggcggc caggccgggc aggtcggccg gcgcgcagcc
     3841 ctgccggtgc atgcccgtgt cgaccttgag gtgggcggcg accgggcggc
//
LOCUS       DASPYY010000679         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_954925, whole genome shotgun sequence.
ACCESSION   DASPYY010000679 DASPYY010000000
VERSION     DASPYY010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954925"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VEP73_10665"
     CDS             <1..511
                     /locus_tag="VEP73_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HYT26932.1"
                     /translation="LAGEVVHVRRHGNQVVIAHAGPEGPAEVARHLITSPGSPRLDEA
                     HYPPAPPGALNREPKPRTAAEAEFLAIGGGAGLWLAEAAAAGASRVRSKMARAVQLAA
                     LHGTEPVDRALGQAAAAGRFADSDLASILAHQASAAPGEPSRAGESRTLAQGTGGWAA
                     RDGRQGEGR"
     gene            508..759
                     /locus_tag="VEP73_10670"
     CDS             508..759
                     /locus_tag="VEP73_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26933.1"
                     /translation="MTAAQPGQPVVLSAQDAAFVRQALVAASMVFAQAKAAGGRGYQA
                     LAEAALKAPDGGRPLARVHYDACLAVDYIDFPMPVRNER"
     gene            819..1535
                     /gene="istB"
                     /locus_tag="VEP73_10675"
     CDS             819..1535
                     /gene="istB"
                     /locus_tag="VEP73_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HYT26934.1"
                     /translation="MRLPHMRRIAPEVIATAKAQRWDPAEVLRALLAEEVAGRDRSSL
                     ATRRTRAAFPTGKTFDAWDERLSSVPAPTQAALRTLEWVGRHENLVVCGPSGTGKTFL
                     LEALGQAAVETGKNVAWFTLEQLGALLRRHRADDSVTKAIARILRADLVVVDDIGLLP
                     VGPDAAEGLYRLVDAAYERRSVAVSSNLHPSGFDELMPKTLATATVDRLMHHAHVCQT
                     SGDSIRLTQATAGQGVIPLN"
BASE COUNT          266 a          731 c          601 g          220 t
ORIGIN      
        1 gctcgcgggc gaggtcgtcc acgtccggcg gcacgggaac caggtcgtca tcgcccacgc
       61 cggcccggaa gggccggccg aggtggcccg gcacctgatc acgtcgccgg gcagcccgcg
      121 cctggacgag gcgcactacc cgccggcccc gcccggggcg ctgaaccgcg agcctaagcc
      181 ccgcaccgca gccgaggcgg aattcctcgc catcggcggc ggcgccgggc tgtggctggc
      241 cgaggccgcc gcggccggag cctcccgggt ccggtccaag atggccaggg cagtccagct
      301 cgccgcgctg cacgggaccg agccggtcga ccgggcgctc gggcaggccg ccgcggccgg
      361 ccgattcgcc gacagcgacc tggcctcgat cctcgcccac caggcatccg cggctcccgg
      421 cgagccgtcc cgggccgggg agtcccgcac cctcgcccag ggcaccggcg gctgggcggc
      481 ccgcgacggc aggcagggag aaggccggtg accgccgcgc agcccgggca gcctgtggtc
      541 ctcagcgcgc aggacgcggc gttcgtccgg caggccctcg tggccgcctc catggtcttc
      601 gcccaggcca aggccgccgg cggccgcgga taccaggccc tggccgaagc agccctcaag
      661 gccccggacg gcgggcgccc cctagcccgg gtgcactacg acgcgtgcct cgcagtcgat
      721 tacatcgact tccccatgcc cgtcaggaac gagcggtgac cgccgccgtc ccggccccgc
      781 cgccgctgga cgacggcctg gagcagctgc tgcgccgcat gcggctgccc cacatgcgcc
      841 gcatcgcgcc cgaggtcatc gccacggcca aggcccagcg gtgggacccc gccgaggtcc
      901 tgcgggccct gctcgccgag gaagtcgccg gacgcgaccg ctcgtcgctg gccacccgcc
      961 gcacgagggc cgcgttcccg accgggaaga cgttcgacgc ctgggacgag cggctgtcct
     1021 cagtcccggc ccccacccag gccgcgctgc ggacactgga atgggttggc cggcacgaga
     1081 acctcgtcgt ctgcggcccg tccggcaccg gcaagacgtt cctgctcgag gccctcggcc
     1141 aggccgccgt cgaaaccggg aagaacgtcg cctggttcac cctcgagcag ctcggcgccc
     1201 tgctccgccg gcaccgcgcc gacgactccg tcaccaaggc catcgcccgc atcctgcggg
     1261 ccgacctcgt cgtcgtcgac gacatcgggc ttctcccggt cggacccgac gccgccgagg
     1321 gcctctaccg ccttgtcgac gccgcctacg agcgccgcag cgtcgcggtc agctccaacc
     1381 tccacccgtc gggcttcgac gagctcatgc ctaagaccct ggccaccgcc actgtcgacc
     1441 ggctcatgca ccacgcccac gtctgccaga ccagcggcga cagcatccgc ctcacccagg
     1501 ccaccgcagg acaaggagtg atccccctga actaaccagc cgggcacgtc tggcggccat
     1561 caacgggcag atctgatggc cgccagcggg cacaactcgt gaccgccacc gggcaactcc
     1621 caatgtccct tgacaggttc cgacaccgat tccgtctcga gcggtcagcg cgacttcacg
     1681 ctgtatctgg aacccacgtt ttgctgccag cccgagcgcc ccacgcaact aggtgcctca
     1741 gaaactttcc cgaagagcga cgtgacgggg tcgttatccg cgatgcgaag ggtattccgg
     1801 cagtgccgat cccatgag
//
LOCUS       DASPYY010000680         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1657429, whole genome shotgun sequence.
ACCESSION   DASPYY010000680 DASPYY010000000
VERSION     DASPYY010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657429"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VEP73_10680"
     CDS             complement(<1..947)
                     /locus_tag="VEP73_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT26935.1"
                     /translation="MTTDLARLRNFCIIAHIDHGKSTLADRLLDLTHAVSERQMRAQY
                     LDKMDLERERGITIKAQAVRLAYTAADGLDYVLNLIDTPGHVDFTYEVSRALAACEGA
                     VLLVDAVQGIEAQTLANLYLALEADLTIIPVINKIDLPAAQPERVAREIEQVVGVPAA
                     DMLRVSAKTGEGVPGLLEAVVRRVPPPRGDPEAPARGLVFDSLYDPYRGVITYVRMVD
                     GRLARNDRVRMMATRGVSEAEEVGVFAPEAVVTADLAAGEVGYLIPGVKNVRQAKVGD
                     TVTTASRPATEPLPGYREPRPMVYSGLYPIEGDDFPALR"
     gene            1193..1459
                     /gene="rpsT"
                     /locus_tag="VEP73_10685"
     CDS             1193..1459
                     /gene="rpsT"
                     /locus_tag="VEP73_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYT26936.1"
                     /translation="MANIKSQIKRNRQNETARLRNKSVRSALKTYARRVRQSLAAGDR
                     DAAEAAYRRAARAYDKAASKGVIHRNNAANHKSRLARALSDEGE"
     gene            complement(1587..>2107)
                     /locus_tag="VEP73_10690"
     CDS             complement(1587..>2107)
                     /locus_tag="VEP73_10690"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26937.1"
                     /translation="LADTVGQDLRELAGAVAKLHVAVPPPTVLTAAHVAEFLSQTADR
                     GVFELTDAVFAGDAATALERLDALLAQGEDALGLLGMLARQLRLLLRVADHPGAPASQ
                     VAQLIGPPTRDWQVERARRQARKFRPEDLRRGLDVIAEADAAVRNGSLPPRLLLELVV
                     TRLAGAGAGARR"
BASE COUNT          288 a          730 c          814 g          275 t
ORIGIN      
        1 tcgcgcaggg cggggaagtc gtcgccctcg atcgggtaga gcccggagta gaccatcggc
       61 cggggctcgc ggtagccggg cagcggctcg gtcgctggcc ggctggccgt ggtgacggtg
      121 tcccccacct tggcctggcg cacgttcttg accccgggga tcaggtagcc gacctcgccg
      181 gcggcgaggt cggcggtgac caccgcctcg ggggcgaaca ccccgacctc ctcggcctcc
      241 gacaccccgc gggtggccat catgcggacc cggtcgttgc gggccagccg gccgtcgacc
      301 atgcggacgt aggtgatgac cccgcggtag gggtcgtaga gcgagtcgaa gacgagcccg
      361 cgggccggcg cctccgggtc gccccggggc ggcgggacgc ggcggacgac cgcctcgagc
      421 aggccgggca ccccctcgcc ggtcttggcc gagacgcgca gcatgtcggc tgctggcacg
      481 ccgaccacct gctcgatctc ccgggcgacc cgctcgggct gcgcggccgg gaggtcgatc
      541 ttgttgatca ccgggatgat cgtcaggtcg gcctcgagcg ccaggtacag gttggcgagc
      601 gtctgcgcct cgatgccctg gacggcgtcg accagcagca cggcaccctc gcacgcggcc
      661 agggcgcgcg acacctcgta ggtgaagtcg acgtgcccgg gggtgtcgat caggttcagg
      721 acgtagtcga gcccgtcggc ggcggtgtag gcgaggcgca cggcctgggc cttgatggtg
      781 atccctcgct cgcgctcgag atccatcttg tccaggtact gcgcgcgcat ctgccgctcg
      841 ctgaccgcgt gggtgaggtc gagcagccgg tcggccagcg tcgacttgcc gtggtcgata
      901 tgggcgatga tgcagaagtt ccgcaggcgc gcgagatctg tggtcatgga cggtggggtc
      961 gtacggtgga ccagggcggc gtcgctccgg ctcgtccggg agcccggtcg ggtggcgagc
     1021 ataccatcgc cgcaggcagg agccgccgcg tgattgagcg gctcggagct ggtgtgctac
     1081 cgtctcccgg ttgtcctcga cggcgcgcgg cggtcccggg atcccgcagg agacggcccg
     1141 cgcccgcgag caccctggat acgtattgcc gcccgcacgg gagaggtact cgttggcgaa
     1201 catcaagtcc cagatcaaac ggaaccggca gaacgagacc gcccggctcc ggaacaagtc
     1261 cgtccgctcc gcgctcaaga cttacgcccg gcgcgtgcgc cagagccttg ccgccggcga
     1321 ccgcgacgcg gccgaggccg cgtaccggcg cgccgcccgc gcctacgaca aggcggccag
     1381 caagggcgtc atccaccgca acaacgccgc caaccacaag tcgcgccttg cgagggcact
     1441 cagcgacgag ggcgagtagg cgacggcggc cgcctgaagt cccgctcgag cggtcgttca
     1501 gcgccggtag agcgcctgca gctcggctgg ggacgggcag aagcaggagc tgaggtcctc
     1561 gcggccgcag ccggcgggca gacctcctat cggcgggcgc cggcgcccgc gccggccagg
     1621 cgggtgacca ccagctcgag gagcaggcgc ggcgggaggc tgccgttacg caccgcggcg
     1681 tcggcctcgg cgatcacgtc gaggccccgg cgcaggtcct cggggcggaa cttcctcgcc
     1741 tggcggcggg cgcgctccac ctgccagtca cgggtggggg ggccgatgag ctgggcgacc
     1801 tggctcgcgg gcgcacccgg atggtcggcg accctgagca gcaggcggag ctggcgggcg
     1861 agcatcccga ggagcccgag ggcgtcctcg ccctgtgcga ggagggcgtc gaggcgctcg
     1921 agcgcggtcg cggcgtcccc ggcgaagacc gcgtcggtga gctcgaacac gccgcggtcg
     1981 gcggtctggc tgaggaactc cgcgacgtgg gcggccgtca gcaccgttgg cgggggcacg
     2041 gcgacgtgca gcttggccac tgcgccggcc agctcgcgaa ggtcctggcc gaccgtgtcg
     2101 gcgagcg
//
LOCUS       DASPYY010000681         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1657442, whole genome shotgun sequence.
ACCESSION   DASPYY010000681 DASPYY010000000
VERSION     DASPYY010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657442"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..800
                     /locus_tag="VEP73_10695"
     CDS             141..800
                     /locus_tag="VEP73_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT26938.1"
                     /translation="MTVPAGAAIAVTGPSGSGKSTLLNLIAGLDRPTGGEVTVLGQAL
                     SALREHELTAFRARHVGFVFQDPHLLPGLTALENVVVARLPWRSRRELEREARELLDA
                     VGLAGRVDHPPARLSGGERQRVGMARALLGGPALLLADEPTGNLDAATTEALLELLGR
                     LRAELTLTLVIATHDPVVAAVADRVVRLVDGQVQSDHATATDAGIRMVTAPDLPTHIL
                     E"
     gene            805..>2605
                     /locus_tag="VEP73_10700"
     CDS             805..>2605
                     /locus_tag="VEP73_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26939.1"
                     /translation="MLWLAVRQLASRRTATALAALGLLTATLGFITLVGTSQTTRAVL
                     RGDIARTWNTPYDLLVRPPGSVTPLEARQGLVRPNYVSGLAKGGITRGQLEAIRHIPG
                     VAIAAPVAIAGAVNWTTGGFGVLLDQEPAPRLLAVYKLVIESTTDAGLSRATVETHYI
                     VVAGEGTVRYVAGTRDATLDLRGRTIDCRYPVSCFAPVECDHGQCGPPADPPNHGVEV
                     LQPIVIAGVDPAAEAALLGLDRCVTRGRALLPGDHLADAGDRDPPGTRIPVLVSARSF
                     VDETFTGHLTRAANAAALLRGAQPQALASWQPVREVKSSADDLYRAYLPRVGEEVDEW
                     PIWSIGDVSYRERPGGLHLEAATRPPDPSIYERRNTSSAIPLSVTTPPAVRDRWFRTV
                     TQHGYDQAEGNRYWTVVGTYNPDCLPGFDPLAGGGLETYATPTVRLADGRELRPSRSL
                     AGYINPPPLLLTTLDGAAWLSDPKRFDGRPGAAFISVVRVKLAGLGEPGPLAEARIGR
                     AAADIRDATGLQVDVVKGASPREVQVDLPAGRFGRPALTVREPWSVKGVAFRFTEAVS
                     AQNLALFTLALVGATILVGETAYTAVRRRRAEFG"
BASE COUNT          342 a         1024 c          908 g          331 t
ORIGIN      
        1 cggagacgcg ggcagccgca ccacgcggcc ggcaggggca aggggccggc gatgacgcgg
       61 ctcccgcctt gcacctcgag gctgtctcgc ggagctatgc gaccggcgcg gccgaggtgc
      121 gcgcagtccg ggaggtgacc atgacggtgc cggccggggc cgccatcgcg gtcaccggcc
      181 cctccgggtc cggcaagagc accctgctca acctgatcgc cggcctggac cggccgacag
      241 gcggggaggt caccgtgctc gggcaggcac tgtcggcgct ccgcgagcac gagctgaccg
      301 ccttccgcgc ccgccacgtc ggcttcgtct tccaggaccc gcacctgctg cccggcctga
      361 ccgcgctgga gaacgtggtg gtggcacggt tgccatggcg gtcccgccgc gagctggaac
      421 gcgaagcccg cgagctgctc gacgccgtcg gcctggccgg gcgggtcgac cacccgccag
      481 cgcgcctgtc gggcggggaa cgccagaggg tcgggatggc ccgagcgctg ctcggcggcc
      541 ccgccctgct gctggccgac gagccgaccg gcaacctgga tgccgccacc accgaggcgc
      601 tgctcgagct gctcggccgg ctccgcgccg agctgacgct caccctggtc atcgccaccc
      661 acgacccagt ggtcgcggcg gtcgccgacc gcgtcgtgcg gctggtggac ggccaggtgc
      721 aatccgacca cgccaccgcc accgacgctg gcattcgcat ggtcaccgcg ccggacctgc
      781 ccacccacat cttggagtga cgccatgctc tggctcgccg tccgccagct cgccagccgg
      841 cgcaccgcca cggcgctcgc cgcgctcggg ctgctgaccg ccacgctcgg cttcattacc
      901 ctggtgggca cctcgcagac gaccagggcg gtgctgcgcg gtgacatcgc ccgcacctgg
      961 aacaccccct acgacctgct cgtccggccg cccggctcgg tcacgccgct ggaggcccgg
     1021 cagggcctgg tacggccgaa ctacgtcagc gggcttgcca aaggcggcat cacccggggc
     1081 cagctcgagg cgatccggca tatccccggc gtggccatcg ccgcgccggt ggcgatcgcc
     1141 ggcgccgtga actggacgac cggcggcttc ggcgtgctcc tcgaccagga acccgccccc
     1201 aggctgctgg cggtgtacaa gctggtgatc gagtcgacca ccgatgctgg gctgtcacgg
     1261 gcgacggtcg aaacccacta catcgtggtg gccggagagg gcacggtgcg ctacgtggcg
     1321 ggaacccgcg acgccacgct ggacctgcgc ggccgcacga tcgactgccg ctacccggtg
     1381 agctgtttcg cgccggtcga gtgcgaccac gggcagtgcg gcccgccggc cgacccgccc
     1441 aaccacggcg tggaggtcct gcagccgatc gtcatcgccg gcgtcgaccc ggccgccgag
     1501 gccgccctgc tcggcctcga ccgctgcgtg acgcgcggcc gcgccctgct ccctggcgac
     1561 cacctcgccg acgccggcga ccgcgacccg ccggggacgc gcatcccggt actggtgagc
     1621 gcacgctcgt tcgtggacga gaccttcacc ggccacctca cccgcgcggc caacgcggcg
     1681 gcgctgctgc gcggcgcgca accgcaggcg cttgcgtcct ggcagccggt ccgggaggtc
     1741 aagagcagcg ccgacgacct gtaccgcgcc tacctgccca gggtcggcga ggaggtcgac
     1801 gagtggccga tctggtcgat cggcgacgtc agctaccggg agcggccggg cggcctgcac
     1861 ctcgaggccg ccacccggcc gcccgacccg tccatctacg agcggcgcaa caccagctca
     1921 gccatcccgc tcagcgtgac caccccgccc gccgtgcgcg accgctggtt ccgcacggtc
     1981 acccagcacg gctacgacca ggccgagggc aaccgctact ggacggtcgt cggcacctac
     2041 aaccccgact gcctgcccgg gttcgatccg ctcgcgggcg gcggtcttga gacctacgcc
     2101 accccaacgg tgcggctcgc cgacggtcgc gagctgcggc caagccgctc gctcgccggc
     2161 tacatcaacc cgccgccgct gctcttgact accctggacg gcgccgcctg gctctcggac
     2221 ccgaaacggt tcgacgggcg gccaggcgcc gcgttcatca gcgtggtccg ggtgaagctg
     2281 gctggcctcg gcgagcccgg cccgctcgcc gaggccagga tcgggcgggc cgccgccgac
     2341 atccgcgacg ccaccggcct gcaggtcgac gtcgtcaaag gcgcgtctcc ccgtgaggtc
     2401 caggtcgatc tgccggctgg gcggttcggc cggcccgcgc tgaccgtgcg cgagccatgg
     2461 tcggtcaaag gggtcgcctt ccgcttcacc gaggcggtca gcgcccagaa cctcgccctg
     2521 ttcaccctcg ccctggtcgg ggcgaccatc ctggtcggcg agaccgccta cacggcagtg
     2581 cgccgtcgcc gtgccgagtt cggcg
//
LOCUS       DASPYY010000682         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2781369, whole genome shotgun sequence.
ACCESSION   DASPYY010000682 DASPYY010000000
VERSION     DASPYY010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781369"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VEP73_10705"
     CDS             complement(<1..580)
                     /locus_tag="VEP73_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26940.1"
                     /translation="MALLVALACLEVGVPRLVGPVAEQVRVVGRLGDPTLEPAGAFLV
                     LLAAATELVAAYLAVVVCLRMLAWLPGTLGRLAAGGIRMLTVPAVRQAVDGLLGGVLV
                     VQLGLSPVVVHADPEGTRAGPRPVAAATAPTTPVPPWLSWPMRPSGGAGPGATAGGGA
                     IPPSTASPAPVAPPSLPPAATRALPPVAVPGQR"
     gene            812..1399
                     /locus_tag="VEP73_10710"
     CDS             812..1399
                     /locus_tag="VEP73_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26941.1"
                     /translation="MRGSEDPPPEPMRRVFDQLEAEFEAGLRREAEQETVAALRAQVG
                     ATAMWEQLARRTGADGVVRAGRRTVRGAVLASYPDFYVLQGTGGAQHLVRFEATSIAL
                     PLEPGPPQPAPDAAVRRYTFGLALRELARRREPVRLELTDGTAINGTVEVVGSDYLEM
                     AEHDIGEARRETAVRARRFAAFTAIAFVTLPPDRR"
     gene            complement(1374..1619)
                     /locus_tag="VEP73_10715"
     CDS             complement(1374..1619)
                     /locus_tag="VEP73_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT26942.1"
                     /translation="MSTPGVEPRFYTLEDVATILNVRVAQVYALVRSGDLPAVKLGGR
                     GVWRVDRNQLEEYIERMHARTREWAKAHPLIGGPEAE"
BASE COUNT          267 a          734 c          684 g          270 t
ORIGIN      
        1 cccgctgccc ggggacggcg acgggcggga gcgcccgggt cgccgccggc gggagcgagg
       61 gaggagcgac cggcgccggc gaggcggtgg acggtgggat ggcgccacct ccggccgtcg
      121 ctcctggccc cgcgccgccg ctcggccgca tgggccatga gagccacggc gggaccggcg
      181 tcgtgggcgc cgtcgcggca gcgacgggac gcggcccggc ccgggtcccc tcggggtccg
      241 cgtgcaccac caccggggac agcccgagct gcacgaccag cacgccgccc agcagtccat
      301 ccacggcctg gcggaccgcg ggcacggtga gcatccgtat gccgccggcc gccaggcggc
      361 cgagggtgcc tggcagccac gcgagcatcc gcaggcagac gacgaccgcc aggtaggcgg
      421 cgaccagctc ggtggccgcc gcgagcagga cgaggaaggc gccggccggc tcgagggtgg
      481 ggtcgccgag ccggccgacg acccggacct gctcggcgac cggtccgacc agacgcggga
      541 cgccgacctc cagacaggcg agcgcgacga gcagggccac cccgcgcacc tgcctgcgcc
      601 ctccccgcgc ctgcctggac cctcggctcc tcgtggagcc tgacgacccg atcccttgct
      661 taccgggtcc ttgcttgtcg agctcatgct catcgatcgc tcgcattcgt tacctccagt
      721 tcgtggccga aataggtacc actcgtcaag gaggccgtca atgctccgcg ttcccattgt
      781 tgacaggtcc gggccccggt aggctcccgt catgcgtggc agcgaggatc cgccgcccga
      841 gccgatgcgc cgggtgttcg accagctcga ggcggagttc gaggccggcc tgcgccggga
      901 agccgagcag gagacggtgg cggccctgcg tgcccaggtc ggggccaccg ccatgtggga
      961 gcagctggcc cgccggaccg gtgccgacgg cgtcgtgcgt gccggcaggc ggaccgtgcg
     1021 cggcgccgtg ctcgccagct acccggactt ctacgtgctc cagggcactg gcggcgccca
     1081 gcacctggtc cgcttcgagg cgacctcgat cgccctgcct ctggagccgg gcccgcccca
     1141 gccggcaccg gacgcggccg tccgccgtta caccttcggc ctcgccttgc gcgagctcgc
     1201 ccggcgtcgg gagccggtgc ggctcgagct caccgacggc accgcgatca acgggacggt
     1261 cgaagtagtc ggcagtgact acctggagat ggccgagcac gacatcggcg aggcccgccg
     1321 cgagacggcg gtgcgagcgc ggcgcttcgc cgccttcacc gccatcgcgt tcgtcactct
     1381 gcctccggac cgccgatgag cgggtgcgcc ttggcccact cccgcgtccg ggcgtgcatc
     1441 cgctcgatgt actcctcgag ctggttgcgg tcgacccgcc ataccccacg gccgcccagc
     1501 ttgacggcgg gaaggtctcc actgcggacc agcgcgtaga cctgggcgac cctgacgttg
     1561 aggatggtgg ccacgtcctc gagcgtgtag aaccgcggct cgacgccggg agtggacatg
     1621 ggagacctcc tgcgctgacc ggtggctgcg ggcacccgac cgaatggctt gtccacagta
     1681 gcagcagccc tgtttgcttt caatagccct gcgcatcatc ctcttgacac cgtttactca
     1741 gcggaggccg atgcgcatat gtccggaact gccagcttgc tgtttcaggc tgggacgcgc
     1801 atcttcatcc cattgcactg gttggagagg tgatcccccc tgtcaactcc caatccgccc
     1861 cctccgcgtt cccagaccgc caccgccgcg acggccgcac gccttggcag gcccaccagg
     1921 cgcaacggca ggctcgcgct cgggctcctc ctcgt
//
LOCUS       DASPYY010000683         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2219474, whole genome shotgun sequence.
ACCESSION   DASPYY010000683 DASPYY010000000
VERSION     DASPYY010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219474"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1046)
                     /locus_tag="VEP73_10720"
     CDS             complement(96..1046)
                     /locus_tag="VEP73_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYT26943.1"
                     /translation="MSQPLADALRGYLQYLTVERGLAASTVESYRRDLRRYAAVLAAR
                     GKTALGDVTPADVAGFLASLREGDAEHGALAVSSAARAVIAVRGLHAFAVAQGLAGTD
                     PAREVQPPAPPRRLPKAITIQDVERLLDAAGPGPDDLSAEPRLLRDRALLEFLYGTGA
                     RISEATGLDVDELQLGEDPVVRLLGKGGKHRVVPVGSYAVRALEAYLVRARPGLAAAS
                     RRASASPAVFLNARGGRLTRQGAWGVLHAAAGRAGLPDVSPHTLRHSFATHLLDGGAD
                     IRVVQELLGHASVTTTQVYTLITVDRLREVYATSHPRALG"
     gene            complement(1043..>1810)
                     /locus_tag="VEP73_10725"
     CDS             complement(1043..>1810)
                     /locus_tag="VEP73_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYT26944.1"
                     /translation="YETVQLPDGSLPLLAPMSEVAGRMAPQVGAHHLQRDGGGRGVLM
                     GGVSGVYAAKVVVLGAGVSGMNAAAIALGMQAEVLLVDRNIARLRQADAIYQGHCQTV
                     ASNAYEIERAVVDADLVIGAVLVPGAKAPRLVTDELVSRMKPGAVLVDIAIDQGGCFE
                     GSRPTTHADPTYRVHDTLFYCVANMPGAVPHTSTYALTNVTLPYAVEMANRGWRDALR
                     ADPALGLGLSTYAGQLTCEPVAEAHALPYTPASEVLN"
BASE COUNT          237 a          659 c          693 g          221 t
ORIGIN      
        1 agaccggcct gacccagtgg atggccgacc acaaagacac cctcggccgc gtttacgcca
       61 acgagctcac ccgtcacttc cgctgaggct ggcggtcagc ccagggcgcg ggggtgggag
      121 gtggcgtaga cctcgcggag cctgtccacc gtgatcagcg tatagacctg ggtggtggtg
      181 accgaggcgt ggccgagcag ttcctggacg acccggatgt cggcgccgcc gtcaagcagg
      241 tgggtggcga acgaatggcg cagcgtgtgc ggcgacacgt cgggcagtcc cgcgcggccg
      301 gcggcggcgt gcagcacgcc ccaggcgccc tgcctggtga ggcggccgcc gcgggcgttg
      361 aggaacacgg cggggctcgc cgacgcgcgg cgggacgccg cggcgaggcc gggccgggcc
      421 cggaccaggt acgcctcgag cgcccgcacc gcgtagctgc ccaccggcac cacccggtgc
      481 ttgccgccct tgccgagcag ccgcaccacg gggtcctcgc cgagctgaag ctcgtcgacg
      541 tcgaggccgg tcgcctcgga gatgcgcgcc ccggtgccgt agagaaactc gagcagcgcg
      601 cggtcccgca gcaaccgggg ctccgcggac aggtcatcgg ggccgggacc ggccgcgtcg
      661 agcaggcgct ccacgtcctg gatcgtgatc gccttgggca gccgccgggg cggcgcgggc
      721 ggctggacct cgcgggccgg gtcggtaccg gccaggccct gggccaccgc gaacgcgtgc
      781 agcccgcgga ccgcgatcac ggcccgcgcg gccgagctga ccgccagcgc cccgtgctcg
      841 gcgtcgcctt cccgcagcga ggcgaggaac ccggccacgt cggccggggt gacgtcgccc
      901 agtgcggtct tgccgcgggc ggccagcacc gcggcgtacc ggcgcaggtc ccggcggtag
      961 gactccaccg tgctggccgc cagcccgcgc tccacggtca ggtactgcag gtagccgcgg
     1021 agcgcgtcag cgagcggctg gctcagttga ggacctcgct ggccggggtg tagggcagcg
     1081 cgtgcgcctc ggcgaccggc tcgcaggtca gctggccggc gtaggtggac aggcccagtc
     1141 ccagggcggg gtcggcgcgg agcgcgtcac gccagccccg gttggccatc tcgaccgcgt
     1201 agggcagcgt cacgttggtc agcgcgtagg tggacgtgtg cggcacggcg ccgggcatgt
     1261 tcgccacgca gtagaacagc gtgtcgtgca cccggtaggt ggggtccgcg tgcgtggtgg
     1321 gccgcgagcc ctcgaagcag ccgccctggt cgatggcgat gtcgacgagg accgcgcccg
     1381 gcttcatccg ggacaccagc tcgtcggtga ccagcctggg cgccttggcc cccggcacca
     1441 gcaccgcgcc gatgaccagg tcggcgtcga cgaccgcgcg ctcgatctcg taggcgttgg
     1501 aggcgaccgt ctggcagtgc ccctggtaga tggcgtcggc ctggcggagc cgggcgatgt
     1561 tcctgtcgac cagcagcact tcggcctgca tgccgagggc gatcgcggcc gcgttcatgc
     1621 cggacacgcc cgcgccgagc acgaccacct tggccgcgta cacgcccgac accccgccca
     1681 tcagcacccc gcgcccgccg ccgtcgcgct gcaggtggtg cgcgccgacc tgcggcgcca
     1741 tccggcccgc gacctcggac atcggggcga gcagcggcag cgacccgtcg ggcagctgga
     1801 cggtctcgta
//
LOCUS       DASPYY010000684         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1940283, whole genome shotgun sequence.
ACCESSION   DASPYY010000684 DASPYY010000000
VERSION     DASPYY010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940283"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VEP73_10730"
     CDS             complement(<1..142)
                     /locus_tag="VEP73_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYT26945.1"
                     /translation="MAYDIRRVAVLGAGTMGAAIAAHAANAGLLVDLLDMAPAGPGPR
                     SGE"
     gene            309..1355
                     /locus_tag="VEP73_10735"
     CDS             309..1355
                     /locus_tag="VEP73_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT26946.1"
                     /translation="MQFDKPVLPWWQSEARGRRDGSIQGYGCPGLSPVAVGLVGAELA
                     RGDGSICTFFGVHSGLAMSSVAMLGSEEQKRRWLPAMARMEKIGAFALTEPRHGSDAV
                     ALETRARRDGDGYVLHGAKRWIGNASFADLVIVWARDDDGKVGGFVVEKGAPGFAASV
                     MTGKIAKRAVWQAELTLDGVRVPAASRLAGSRSFADTGRVLTATRYGVAWEAVGHAAA
                     AYEAALAYAKERTQFGTPLAGFQLVQSKLAHMLAELTSMQLLCLRLSQLAADGRMTGG
                     MASLAKMNNAAKARRIVADARDILGGNGILLENHVARHQADMEAVFTYEGTDSVQSLI
                     LGREITGESAFTKR"
     gene            complement(1422..>2798)
                     /locus_tag="VEP73_10740"
     CDS             complement(1422..>2798)
                     /locus_tag="VEP73_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT26947.1"
                     /translation="MLIASRVLQGLMGAIMFPQVLSVIQVSFPPRERATALGIFGATI
                     GVATIAGPLVGGLLIQANLLGLDWRPIFLVNVPIGIASLTAAAFLLRESRSADARRLD
                     LGGVALVTAGLLLLVYPLVQGRELGWPRWTFLSMAASVPVLALFALYERRKKRRDGSP
                     LVDLDLFRQRPFVSGLLLSLIFFMGISAFFLTFTIYLQIGLGFTALHAGLTTVPFAIF
                     SAIASGASVRLAPRLGTRVLHLGTLLLTSGMGAVILTIHRYGTSLRSWQLIPALALCG
                     LGLGSVVAPLVNLVLAGIRREDAGAASGLLTTVQQVGGSIGVAIIGVIFFGLLASHAD
                     TASAEVAPQLRAGLQAAGVPAGAREGVVLSFRRCFHDRSASHDPSATPPSCDQSRPQG
                     GGQAGQRAGGVAQAAAVEARKRDFSEAIQRTLLFEVAVFAASFLLVFTLPKVRGEALR
                     QQGATVAA"
BASE COUNT          439 a          971 c         1072 g          316 t
ORIGIN      
        1 cctccccgga gcgggggccc ggcccggccg gggccatgtc gagcaggtcg acgagcaggc
       61 cggcgttggc ggcgtgggcc gcgatggcgg cccccatggt ccctgcgccg agcacggcga
      121 ccctgcggat gtcgtatgcc attcgaaacc tccagcgcgg cgggcggtca ccccaacgat
      181 accggttccc ggggccgcct ggccacggcc ggcgacgacg ccgacgtcgg cgtcgagcga
      241 aaagcagttc cggctccgac gtcggcgtcg ggtaaatgag aagccccgac gctgacgtcg
      301 gcgtcgaaat gcaattcgac aaaccggtgc taccctggtg gcaaagcgaa gcgagaggac
      361 ggcgtgacgg cagcatccag gggtacggat gtcccgggct gtccccggtc gccgtcggcc
      421 tcgtcggcgc ggagctggcg cgcggcgacg gcagcatctg cacgttcttc ggcgtccact
      481 cgggcctcgc gatgtcgtcg gtcgcgatgc tcggctccga ggagcagaag cggcgctggc
      541 tgccggccat ggcccgcatg gagaagatcg gcgcgttcgc gctcaccgag ccgcggcacg
      601 gctcggacgc ggtggcgctg gagacccgcg ccaggcgcga cggcgacggc tacgtgctgc
      661 acggcgccaa gcgctggatc ggcaacgcct ccttcgccga cctcgtgatc gtgtgggcgc
      721 gcgacgacga cggcaaggtg ggcggcttcg tggtcgagaa gggcgcgccg ggcttcgcgg
      781 ccagcgtgat gaccggcaag atcgccaagc gcgcggtgtg gcaggccgag ctcaccctcg
      841 acggcgtccg cgtcccggcc gccagccggc tcgccgggtc gcgctcgttc gccgacaccg
      901 gcagggtgct gaccgcgacc cgctacggcg tcgcctggga ggcggtcggg cacgcggcag
      961 ccgcctacga ggccgcgctg gcgtacgcca aggagcgcac ccagttcggc accccgctcg
     1021 ccgggttcca gctcgtccag tcgaagctcg cccacatgct ggccgagctc acctcgatgc
     1081 agctcctctg cctgcggctc tcccagctcg ccgcggacgg ccggatgacc ggggggatgg
     1141 cctcgctggc gaagatgaac aacgcggcga aggcgcgcag gatcgtggcg gacgcccgcg
     1201 acatcctggg cggcaacggc atcctgctgg agaaccacgt cgcccgccac caggccgaca
     1261 tggaggcggt gttcacctac gaggggaccg acagcgtcca gtcgctgatc ctcggccggg
     1321 agatcaccgg cgagagcgcc ttcaccaagc gctgacccgc gcctcggggg tcgatctcag
     1381 gcggcgcgta cggcatcccg cccgcgcccg gctcgccggg gtcaggcggc gaccgtcgcg
     1441 ccctgctgcc ggagcgcttc gccgcgcacc ttcgggaggg tgaacacgag caggaagctg
     1501 gccgcgaaca ccgccacctc gaacagcagg gtgcgctgga tcgcctcgga gaagtcccgc
     1561 ttgcgggcct cgacggcggc cgcctgcgcg accccgccgg cgcgctggcc ggcctgcccg
     1621 ccgccctgcg ggcgcgactg gtcgcagctt ggcggggtcg ccgagggatc gtgcgacgcc
     1681 gagcggtcgt ggaagcagcg gcggaaggac aagaccacgc cctcgcgcgc gccggcgggc
     1741 accccggccg cctggaggcc ggcgcggagc tgcggggcga cctcggccga cgccgtgtcg
     1801 gcgtggcttg cgagcaggcc gaagaagatc accccgatga tcgcgacgcc gatcgagccg
     1861 ccgacctgct gcacggtggt gagcagcccg gaggcggccc cggcgtcctc ccggcggatg
     1921 ccggcgagga cgaggttgac gagcggggcg accaccgagc cgagccccag cccgcacagc
     1981 gcgagcgccg ggatgagttg ccaggagcgc aggctggtgc cgtagcggtg gatggtgagg
     2041 atcaccgcgc ccatccccga ggtgaggagc agggtgccga ggtggagcac ccgcgtgccg
     2101 agccgtggcg ccagccgcac ggacgcgccc gacgcgatgg ccgagaagat ggcgaacggc
     2161 acggtcgtga gcccggcgtg cagcgcggtg aagcccagcc cgatctgcag gtagatcgtg
     2221 aacgtcagga agaacgccga gatgcccatg aagaagatga ggctgagcag caggccgctg
     2281 acgaacggac gctggcggaa caggtcgagg tcgaccaggg gagagccgtc gcgccgcttc
     2341 ttccgccgct cgtagagggc gaagagcgcg aggacgggca ccgaggcggc catcgagagg
     2401 aaggtccacc tgggccagcc gagctcgcgc ccctggacca gagggtagac gagcaggagc
     2461 aggccggcgg tgacgagcgc cacgccgccg aggtcgagcc ggcgcgcgtc cgccgacctc
     2521 gactcgcgca gcaggaaggc cgccgcggtg agcgaggcga tcccgatcgg cacgttgacg
     2581 aggaagatcg gccgccagtc gagccccagg aggttcgcct ggatgagcag gccgccgacc
     2641 agggggccgg cgatggtggc caccccgatg gtggcgccga agatgccgag ggcggtcgcc
     2701 cgctccctgg gcgggaacga gacctggatg accgacagga cctgggggaa catgatcgcg
     2761 cccatgagcc cctgcaggac gcgggacgcg atcagcat
//
LOCUS       DASPYY010000685         6770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1657601, whole genome shotgun sequence.
ACCESSION   DASPYY010000685 DASPYY010000000
VERSION     DASPYY010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657601"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VEP73_10745"
     CDS             <1..152
                     /locus_tag="VEP73_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase"
                     /protein_id="HYT26948.1"
                     /translation="LQKLEALDKKTAFAHLERPFDPDAPLFIDYAEHLRAYEGTDRAP
                     APRDR"
     gene            161..478
                     /locus_tag="VEP73_10750"
     CDS             161..478
                     /locus_tag="VEP73_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26949.1"
                     /translation="MQPLKLELALPPEPAELRARTWALAGQDPAGVTDLLGRGDWVAD
                     PLWRCWGPALEPAGMGRDQLRRIAVDYRRELWFWVMGERTWAQCVAGLLGRVRRRLPE
                     SSP"
     gene            560..1357
                     /locus_tag="VEP73_10755"
     CDS             560..1357
                     /locus_tag="VEP73_10755"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HYT26950.1"
                     /translation="MISVLAHSVAEPLLPGMSEARDGVVLGGGDGALYARFEDMVVAV
                     TARGIGRMPNGVSVGVAPDTLARVRAGDAARVWPGGLAAGPMEVLWDPDAPPSWSPRL
                     ADVPPDDGPAIRQRLRHPSLQQDVPAEELRALGGAVASRDPSLAGMLGETFVGAGSGL
                     TPRGDDLLAGTALALRALGRASGWSHREVDAWVAKLLPPELPARTTALSASLLRLAVA
                     GDALEPARRLLDPAEPDPRAAMLSLLGIGRSTGRSYAAAMRVVASTA"
     gene            complement(1396..3093)
                     /locus_tag="VEP73_10760"
     CDS             complement(1396..3093)
                     /locus_tag="VEP73_10760"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYT26951.1"
                     /translation="MPNRRRVVLVLLLSVTAALVLPTLARAQTPEAKVSVAGPTTPYL
                     PNAVNEPALAIDANATSVLAAGANDLVDSAPCKGSSCDLSPDIGISGIYFSLDEGATW
                     VQPTYTGLTARSGTTQVGPIHTLPGYFENGMSSHGDPALAFGPRPGPGGFSWANGSRL
                     YYANLAFPLTGQAPFKGGSAVAVSRTDNVPAAAAGGADGKAAWMPPVVVSKQSAATFS
                     DKEDIWADNAASSSFFGNVYTCNVAFRSVGGAPEPVVVSASRDGGSTWRQRQLSAATN
                     NAQTGGRQGCAVRSDSTGVVYVVWEGADIQTRQSVFYLARSFDGGNNFEKPRAVANVV
                     DVGLPDPVSGRIVFDGYAGTRTDSFPSLGIANGAPTGAGATNTLVLAWPDARAGLNHE
                     QALVQTSTDQGVTWSAPSNAAEAGDRPDNPAVAISPTGSDLYLVYNGYLQPFQTTTAN
                     PRRMQGVVRHADVGAGGALGTWSTLHRAAVGDARGTTRTLNREAVYDYNYAAATRTYG
                     AAAWMDVRDAADCPAVDAYRQSLIDGTPLPAPAPLTDCPPQFGNNDIYGGTYLDPTP"
     gene            3281..3886
                     /locus_tag="VEP73_10765"
     CDS             3281..3886
                     /locus_tag="VEP73_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26952.1"
                     /translation="MFIQVIQGRASNPDGVRAALDRWKAELAPGAEGWLGSTGGVTDD
                     GRCIVLARFESEELARRNSDRPEQGAWWAETSEQVSEPAFHDSARVHAYKGGGSDRAG
                     FVQVIQGHTDDMERLAELGRAQEQRLAEQAPHILGMTVAEHADRPGDFTQTVYFTSEE
                     DARRFEQQRPARSDPASRELFSLMTGLEYFDLRDPWLDSRG"
     gene            3995..4432
                     /locus_tag="VEP73_10770"
     CDS             3995..4432
                     /locus_tag="VEP73_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26953.1"
                     /translation="MSRPRAGFLLLLVLAGCTGPVRSYPVYESKAARTAKVEASAVET
                     AGLAVQTAVERKVFGRTLAQTLAEAARDAGETQGVFDAIQPPDGRADRLRASLDDLLG
                     QTVATLDGLRTASRRGDVAELPKLAAPLADLSRKLDDFAQAHQ"
     gene            4429..5658
                     /locus_tag="VEP73_10775"
     CDS             4429..5658
                     /locus_tag="VEP73_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYT26954.1"
                     /translation="MRKLLEIALGILTAIGGFVDIGDLVASTETGARFGLGLAWVIVL
                     GVIGICVYAEMAGRVAAVSGRAVFDLVRDRLGAGVALVNLFASFFITLLTLAAEIGGV
                     ALALELATSVNYLLWVPVVAFLVWLVIWRVHFEVMENLFGLMGLALVVVAVAVWRAHA
                     DWGGLLHGATRPVVPKGEGHPTYFYFAIALLGSAMTPYEVFFFSSGAVEQRWTRADLM
                     VNRANVYIGFPLGGLLALALMIGAALYLEPRGIDPGHLSQAALPTTLVLGKLGLAVLL
                     LGFFACTFGAALETGLSCGYTIAQYFGWQWGKFVKPAEAATFHLVLLLSVVLGAGLLL
                     TTWDPVKITEYSIVLSAAALPLTYFPVLVVANDRTYMGDKVNSRLSNAIASVFLVLLV
                     VVAVATIPLMVLTKAGS"
     gene            5663..6058
                     /locus_tag="VEP73_10780"
     CDS             5663..6058
                     /locus_tag="VEP73_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26955.1"
                     /translation="MAGRTIDAGLRLLDRQLVDKDGRLAGKVDDLEFTTADGDGAPPY
                     LTAILAGPGALAGRLGGRVGGWLAGLSERLRGSDRDRPASIWFGAVKEIGSAVELSVA
                     RQEVETNVVESWVREHVIGKLPGADHAAE"
     gene            6045..6386
                     /locus_tag="VEP73_10785"
     CDS             6045..6386
                     /locus_tag="VEP73_10785"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HYT26956.1"
                     /translation="MRLSELLGSEVVDEQGGSAGKVHDLRLEQDGPVIGGFGARFRLA
                     GLIVGRRALGARLGYDRGDMKGPWLVKAVAGSLHRGGRYVEWGRVRSVEPGRILISGR
                     AGDLPHPGPLD"
BASE COUNT          846 a         2408 c         2522 g          994 t
ORIGIN      
        1 ccctccagaa gctggaagcg ctcgacaaga agaccgcctt cgcgcatctg gagcggccct
       61 tcgacccgga cgcgccactg ttcatcgact acgccgagca cctccgggcc tacgagggga
      121 ccgaccgggc tccggcgccg cgcgaccggt gagggcggcg atgcaacctc tgaagctgga
      181 gctggcgttg ccgccggagc ccgccgagct gcgcgcccgc acgtgggcgc tcgccgggca
      241 ggaccccgcc ggcgtcaccg acctgctcgg ccggggagac tgggtcgccg acccgctctg
      301 gcgctgctgg ggaccggcgc tggaaccggc ggggatgggc agggaccagc tgcgccggat
      361 cgccgtcgac taccggcgag agctgtggtt ctgggtgatg ggggagcgga cctgggccca
      421 gtgcgtcgcc gggctgctcg gccgggtccg tcgccgcctg ccggagagtt ccccgtagcg
      481 gcacggcaga ccgtggctgc gggaatcgcg ctatcatccg ctccaccacg tgtatgacag
      541 gtagcgaggc gatgggtcgt tgatctccgt cctcgcccac tcggttgccg agccgctcct
      601 ccccgggatg agcgaagcgc gcgacggcgt ggtcctcggc ggcggggacg gcgcgctgta
      661 cgcgaggttc gaggacatgg tcgtggcggt gaccgcccgc gggatcggtc gcatgccgaa
      721 cggggtctcc gtcggggtcg cgccggacac gctcgcccgg gtccgcgcgg gggacgccgc
      781 gcgggtctgg ccgggcgggc tcgccgccgg cccgatggag gttctctggg acccggacgc
      841 gccgccgtcc tggagccccc ggctcgcgga cgtcccgccg gacgacggcc cggcgatccg
      901 gcagcggctg cgccacccct ctctccaaca agacgtgccc gccgaggagc tgcgtgcgct
      961 cggcggcgcc gtggcgtcgc gcgacccgag cctcgccggg atgctcgggg agacgttcgt
     1021 cggggccggc agcggcctga ccccccgcgg cgacgacctg ctcgccggga cggccctggc
     1081 ccttcgtgcg ttgggtcgtg cgagcggctg gagccaccgg gaggtcgacg cctgggtggc
     1141 gaagctgctg ccgcccgagc tccccgcccg gacgacggcg ctgtcggcgt ccctgctgcg
     1201 gctggccgtg gcgggcgacg cgctcgaacc ggcgcggcgg ctgctcgatc ccgccgaacc
     1261 cgacccccga gcggcgatgc tgagcctgct cgggatcggg cgctcgacgg gacgctccta
     1321 cgccgcggcc atgcgcgtcg tggcttcgac ggcgtgaggg gcgcccggag gcgcccctca
     1381 ccagcagccg cgccgtcagg gcgtggggtc gaggtaggtg ccgccgtaga tgtcgttgtt
     1441 gccgaactgc ggcgggcagt cggtcagcgg ggccggtgcg ggcagcgggg tgccgtcgat
     1501 cagcgactgc cggtaggcgt cgaccgctgg gcagtcggcc gcgtcgcgca cgtccatcca
     1561 cgccgccgcg ccgtaggtgc gggtggcggc ggcgtagttg tagtcgtaga ccgcctcccg
     1621 gttcagcgtc ctggtggtgc cgcgggcgtc cccgacggcc gcgcggtgca aggtcgacca
     1681 ggtgccgagc gccccgccgg cgcccacgtc ggcgtggcgg accacgccct gcatgcgccg
     1741 agggttggcc gtggtcgtct ggaacggttg caggtagccg ttgtagacca ggtagaggtc
     1801 gctgccggtg ggcgagatcg ccaccgccgg gttgtcgggc cggtcacctg cttcggcggc
     1861 gttgctcggg gccgaccagg tcaccccctg gtcagtggag gtctgcacca gggcctgctc
     1921 gtgattgagc ccggcgcggg cgtctggcca ggccagcacc agggtgttgg tggcgccagc
     1981 acctgtgggg gcgccgttgg cgatccccag gctcgggaag ctgtcggtgc gggtccccgc
     2041 gtacccgtcg aacacgatcc gccccgacac cgggtcgggt agcccgacgt cgaccacgtt
     2101 ggcgaccgct cggggcttct cgaagttgtt accaccgtcg aaggagcggg ccaggtagaa
     2161 gacgctctgg cgggtttgga tgtcggcgcc ctcccacacc acatagacga cgccggtgct
     2221 atcggagcgg accgcgcagc cctgccggcc gccggtctgg gcgttgttgg tggccgcgct
     2281 gagttgccgc tgccgccagg tgctgccgcc gtcgcgggag gccgagacca ccaccggctc
     2341 gggtgctcca cccacgctgc ggaaggcgac gttgcatgtg tagacgttgc cgaagaagct
     2401 gctggaggcg gcgttgtcgg cccagatgtc ttccttgtcg ctgaaggtcg ccgccgactg
     2461 cttggacacc accaccggcg gcatccacgc cgccttgccg tccgcgccgc cggccgcggc
     2521 ggccggcacg ttgtcggtgc gcgacaccgc aaccgccgaa ccgcccttga acggtgcctg
     2581 accggtgagt gggaaggcca ggttggcgta gtacagccgc gagccgttag cccaggagaa
     2641 cccgcccggg ccgggccgcg ggccgaacgc cagcgccggg tcgccgtggg aggacatccc
     2701 gttctcgaag tacccgggca gggtgtggat cgggcccacc tgggtggtgc cgctgcgcgc
     2761 ggtcagcccg gtgtaggtcg gctgcaccca cgtcgcgccc tcgtcgaggg agaagtagat
     2821 ccctgagatg ccgatgtcgg ggctgaggtc gcagctgctg cccttgcacg gggcgctgtc
     2881 gacgaggtcg ttggcgccgg cggccagcac cgaggtggcg ttggcgtcga tcgccagggc
     2941 gggctcgttg acggcgttgg gcaggtaggg ggtggtcggt cccgccaccg acaccttggc
     3001 ctcaggggtc tgcgcgcggg ccagcgtggg gaggaccagg gccgccgtta ccgacaacag
     3061 cagcaccagc accactcggc gacgattggg catcgcggct ccttccgtcg ttggacgccg
     3121 gatgggagca tatcgggctg tcaaccccca gcctaccttt gcccgaccga gcgcctcgac
     3181 cgtcacgatc ctgccggtct ttcgggttgc tcctcttgac cgggccgagc acgaagctac
     3241 agttgcccgg ggcgacatcc ggctacctcg gggaggcagc gtgttcatcc aggtcatcca
     3301 gggccgagcc agcaacccgg acggcgtccg cgccgcactg gaccgctgga aggccgagct
     3361 cgcaccgggc gccgaaggct ggctcggctc gaccggcggg gtcaccgacg acgggcgctg
     3421 catcgtcctg gcccgcttcg agtccgagga gctggcccgc cgcaacagcg accgtcccga
     3481 gcagggcgcc tggtgggccg agacgtccga gcaggtgagc gagcccgcct tccacgactc
     3541 ggcccgcgtg cacgcctaca aggggggcgg gtcggaccgg gccggcttcg tccaggtcat
     3601 ccagggccac accgacgaca tggagcgcct ggccgagctc ggccgcgctc aggagcagcg
     3661 gctcgccgag caggccccgc atatcctggg gatgaccgtg gccgagcacg ccgaccggcc
     3721 cggggacttc acccagacgg tgtacttcac ctcggaggag gacgcccggc ggttcgagca
     3781 gcagcgcccg gcccgctcgg accctgccag ccgcgagctg ttcagcctga tgaccggcct
     3841 cgagtacttc gacctgcgcg acccctggct ggacagccgc ggctagccgc accgggaacg
     3901 gcgggcgagg cgtccggcgc cgccatgggc ggcggccgcg atggctgggt cggttggttt
     3961 cctcgctgga gggtgcgggt agtgcgacgg cgccatgtca cgaccgcgcg cagggttcct
     4021 gctgctcctg gtgctcgccg gctgcacggg gccggtgcgc tcctatcccg tgtacgagtc
     4081 gaaggcggcc agaacggcaa aggtggaggc gtcggcggtg gagaccgccg ggctggccgt
     4141 gcagacggcg gtcgagcgca aggtcttcgg gcgcaccctg gcgcagacgc tggccgaggc
     4201 cgccagggac gcgggcgaga cccagggcgt cttcgatgcc atccagccgc ccgacggccg
     4261 cgccgaccgg ctccgtgcca gcctggacga cctgctcggc cagacggtcg cgacgctgga
     4321 cggcctccgg accgcgagcc gccgggggga cgtggccgag ctcccgaagc tggccgcgcc
     4381 gctggctgat ctctcccgga agctcgacga cttcgcccag gcgcaccagt gaggaagctc
     4441 ctggagatcg ccctcgggat cctgaccgcc atcggcggct tcgtcgacat cggcgacctg
     4501 gtcgcctcca ccgagaccgg ggcccgcttc ggcctcggcc tggcctgggt catcgtcctg
     4561 ggcgtgatcg ggatctgcgt ctacgccgag atggccgggc gggtggcggc ggtctccggc
     4621 cgggccgtgt tcgacctggt ccgtgaccgc ctcggcgccg gagtcgccct ggtgaacctc
     4681 ttcgcctcct tcttcatcac cctgctcacc ctggcagccg agatcggcgg cgtcgcgctc
     4741 gccctggagc ttgcgaccag cgtcaactac ctgctgtggg tcccggtcgt ggccttcctg
     4801 gtgtggctgg tcatctggcg ggtccacttc gaggtcatgg agaacctgtt cgggctcatg
     4861 ggcctggcgc tggtcgtcgt cgcggttgcc gtgtggcgcg cccacgccga ctggggcggc
     4921 ctcctccacg gcgccacccg gcccgtcgtg cccaagggcg agggccaccc gacctacttc
     4981 tacttcgcga tcgccctgct cggcagcgcc atgaccccct acgaggtgtt cttcttctcc
     5041 tccggggcgg tcgagcagcg ctggacccgc gccgacctga tggtgaaccg ggccaacgtc
     5101 tacatcggct tcccgctcgg cgggctgctc gcactggcgt tgatgatcgg ggcggcgctg
     5161 tacctggagc cacgcggcat cgacccgggc cacctctccc aggccgccct gccgaccacc
     5221 ctggtcctcg gcaagctcgg cctcgccgtc ctgctgctcg gcttcttcgc ctgcaccttc
     5281 ggcgccgccc tcgagaccgg cctgtcgtgc gggtacacga tcgcgcagta cttcggctgg
     5341 cagtggggca agttcgtgaa gcccgccgag gccgccacgt tccacctcgt cctgctgctg
     5401 tcggtcgtcc tcggtgccgg gctgctcctg accacctggg acccggtcaa gatcaccgag
     5461 tactcgatcg tgctgtcggc cgcggcgctg ccgctcacct acttcccggt cctggtggtc
     5521 gccaacgacc gcacctacat gggcgacaag gtcaactcca ggctgagcaa cgcgatcgcc
     5581 tcggtcttcc tggtcctgct cgtcgtggtc gcggtcgcca ccatcccgct catggtcctc
     5641 accaaggcag ggagctgatc tcatggccgg acgcacgatc gacgccgggc tgcggctgct
     5701 cgaccggcag ctcgtcgaca aggacggcag gctcgccggc aaggtcgacg atctcgagtt
     5761 caccaccgcc gacggggatg gtgcaccgcc gtacctgacc gcgatcctcg ccggccccgg
     5821 ggcgctggcc gggcgtctgg gcggccgtgt cggagggtgg ctggcaggcc tcagcgagcg
     5881 actgcgaggc agcgaccggg accggcccgc cagcatctgg ttcggggccg tgaaggagat
     5941 cggcagcgcc gtcgagctca gcgtcgccag gcaggaggtc gagacgaacg tggtggagtc
     6001 gtgggtgcgc gagcacgtga tcggcaagct cccgggagcc gaccatgcgg ctgagtgagc
     6061 tcctcggcag cgaggtcgtg gacgagcagg gcgggtcggc cgggaaggtc cacgacctgc
     6121 gcctcgagca ggacggcccg gtgatcggcg gcttcggcgc gcggttccgg ctcgccgggc
     6181 tgatcgtcgg caggcgcgcg ctcggcgccc gcctcggcta cgaccgtggc gacatgaagg
     6241 ggccgtggct ggtcaaggcg gtcgccgggt cgctccaccg cggcggccgc tacgtggagt
     6301 gggggcgcgt ccggtcggtc gagcccggcc ggatcctgat ctccggccgg gccggcgacc
     6361 ttccccaccc cggcccgttg gactgacccg tcgttcgccg cactccggcg ctgcgcccca
     6421 cctgcccaca aggactcctc gtgccggccg ttgaccctcg gcgggccgag gtgccagggt
     6481 tgtcatggtg caagccaagc acccatgagg gggaagacct gatgacaacg cagccgccct
     6541 ggggacagga ccagccagag cagccgccgg gagcggagtc cgaagcgccg acccaggagc
     6601 tcccggtgtc ggggccccct ggcacgggac tccatcaggc gaccaggcgc atgcgacggc
     6661 cggccggggg cgtgccgccg ggtgaccggt cgagggccgt gctcgctgct ggggttgccg
     6721 ccgccgccgc gctgctgctt gccggcgccg tggcgggatc cctgatcacc
//
LOCUS       DASPYY010000686         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_536503, whole genome shotgun sequence.
ACCESSION   DASPYY010000686 DASPYY010000000
VERSION     DASPYY010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536503"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /gene="glmU"
                     /locus_tag="VEP73_10790"
     CDS             complement(<1..1326)
                     /gene="glmU"
                     /locus_tag="VEP73_10790"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HYT26957.1"
                     /translation="MVAVGGVLMGLSVVVLAAGEGKRFRSSTPKPLHAAAGRALLWHV
                     LAAAAGLRADSTVVVVGRGADEVRTAVAGFSLGPVAFAVQHEQKGTGDAMAAAVPLLP
                     PEGDVLVLYGDTPLITQATLEQLLATHRTLRAGVTLLTCEAQDPTGYGRVLRGPDGQV
                     TGVVEQRDATQAQRAIREVNAGFYVFSRPVLDGLAQLRADNDQGEYYLPDLVPFVLAR
                     GGVVAACRGSEAETPGVNDRAELADAGTVLRRRLLDGLMASGVTVVDPSTTYVDVDVE
                     VGPDTTLEPLTFLEAGTRIGARCRIGPNVRLTGCTVEEEAVVTQTVGVGAHVGPGAQV
                     GPFAYLRPDAVLAEGAKAGTFVELKNARVGPGSKVPHLSYRGDAVIGRHVNVGAGSIT
                     CNYDGYEKHRTTIGDESFIGSDTMLIAPVRLGKRTWTGAGSVIGKDVPAG"
     gene            complement(1340..1412)
                     /locus_tag="VEP73_10795"
     tRNA            complement(1340..1412)
                     /locus_tag="VEP73_10795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1377..1379),aa:Gln,seq:ttg)
     gene            complement(1511..>1887)
                     /locus_tag="VEP73_10800"
     CDS             complement(1511..>1887)
                     /locus_tag="VEP73_10800"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYT26958.1"
                     /translation="IDATGLATQAVYRHYDELGLADPLGDRWPGRLLDALAGGDLARL
                     GASLANDLEPAAFDLLPGLRAGKERLLEAGVLGAVMSGSGPTLLGLCRDEEHAGRVAR
                     AVRSGFARVEVARGPVPGVTFG"
BASE COUNT          244 a          737 c          678 g          228 t
ORIGIN      
        1 ccccgcgggg acgtccttgc cgatcaccga ccccgccccg gtccacgttc gcttgcccag
       61 ccgaacgggt gcgatcagca tcgtatccga gccgatgaag gattcgtcgc cgatcgtcgt
      121 gcggtgcttc tcgtacccgt catagttgca ggtgatcgac cccgccccca cgttcacgtg
      181 ccggccgatc acggcgtctc ccctgtacga gaggtgcgga accttgctcc ccggccccac
      241 ccgcgcgttc ttcagctcca cgaacgtccc ggccttggcg ccctcggcca ggacggcgtc
      301 cgggcgcagg taggcgaacg ggccgacctg ggcgccgggg ccgacgtggg ccccgacgcc
      361 gaccgtctgg gtgacgaccg cctcctcctc gaccgtgcag ccggtgaggc ggacgttcgg
      421 cccgatgcgg cagcgggccc cgatgcgcgt gccggcctcg aggaacgtca gcggttcgag
      481 cgtggtgtcc ggtccgacct cgacgtccac gtcgacgtag gtggtggacg ggtcgaccac
      541 ggtcaccccg gacgccatca gcccgtcgag cagccgccgg cggagcaccg tcccggcgtc
      601 cgccagctcg gcccggtcgt tgaccccggg ggtctccgcc tcggacccgc ggcaggcggc
      661 gaccacgccc ccgcgggcca gcacgaacgg cacgaggtcg ggcaggtagt actcgccctg
      721 gtcgttgtcg gcccggagct gggcgagccc gtcgaggacc gggcggctga agacgtagaa
      781 gccggcgttg acctcccgga tggcgcgctg cgcctgggtc gcgtcccgct gctcgaccac
      841 cccggtgacc tgcccgtccg ggcctcgcag gaccctgccg tagcccgtcg ggtcctgcgc
      901 ctcgcaggtg agcagggtga cgccggcccg aagcgtgcgg tgggtggcga ggagctgctc
      961 cagcgtggcc tgggtgatga gcggggtgtc gccgtagagc acgagcacgt cgccttcggg
     1021 ggggagcagg ggcacagcgg cggccatcgc gtcgccggtg cccttctgct cgtgctggac
     1081 ggcgaacgcc accgggccga ggctgaatcc ggcgaccgcc gtgcgcacct cgtcggcgcc
     1141 ccgcccgacc accaccacgg tcgagtccgc ccgcagcccg gccgccgccg ccagcacgtg
     1201 ccacagcagc gcccgcccgg ccgcggcgtg cagcggcttg ggcgtcgacg agcggaagcg
     1261 cttcccctcc cccgccgcga gcacgaccac gctcaggccc atcagcaccc ctccgacggc
     1321 gaccacgaga accccactgc tggcgggcag ggattcgaac cccgacctga cggctccaaa
     1381 ggccgccgtg ctgccgatta caccacccgc caacgctgca ccggcatcgt accgaaccgc
     1441 cccatcgcct gaggcgcgcg aggcccgctc gcctgaggcg cgccgggccc ggctgcctga
     1501 agcgcgacgc ctagccgaag gtcacgccgg ggaccgggcc gcgggcgacc tcgacgcggg
     1561 cgaagccgga ccgcacggcc cgcgccaccc tgccggcgtg ctcctcgtcg cggcacagac
     1621 cgagcagcgt cgggccgctc ccgctcatga cggcgccgag gacgcccgcc tcgagcagcc
     1681 gctccttgcc ggcccgcagg ccgggcagga ggtcgaacgc cgccggctcg aggtcgttcg
     1741 cgagggaggc gccgagccgg gcgaggtcgc cgccggcgag cgcgtcgagc agccggcccg
     1801 gccaccggtc gccgaggggg tcggcgagcc ccagctcgtc gtagtggcgg tacaccgcct
     1861 gcgtggcgag gccggtcgcg tcgatgc
//
LOCUS       DASPYY010000687         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2079248, whole genome shotgun sequence.
ACCESSION   DASPYY010000687 DASPYY010000000
VERSION     DASPYY010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079248"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VEP73_10805"
     CDS             complement(<1..235)
                     /locus_tag="VEP73_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26959.1"
                     /translation="MTRARPAPAGPGHVAERARPGAPGVGARLGVVAAGVAFLLLRPA
                     LWELPGAAAVLAAGYLALAAAAAMGAGGDGAWPE"
     gene            complement(232..675)
                     /locus_tag="VEP73_10810"
     CDS             complement(232..675)
                     /locus_tag="VEP73_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26960.1"
                     /translation="MRSGRPGATVAVVLALAAACLAGCGKSEAKPPATSPPATLGARP
                     SSPAKLAILSPRNGQKVRGGAITLRLRLDNARIVNVTSTRVRPDQGHVHVLVDGRLVA
                     MNYRLTGRLPALPPGQHVVRVDFVGSDHLPFDPQVSTRSAFQVER"
     gene            complement(684..947)
                     /locus_tag="VEP73_10815"
     CDS             complement(684..947)
                     /locus_tag="VEP73_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26961.1"
                     /translation="MTPGKPGTNQIHVTFFDARGNEAPQADVKVTATPPSGRAPALRV
                     LRFSPGHVAAQGELGPGRWTFDIAATARDGTASTARFEQAIGK"
     gene            complement(944..2704)
                     /locus_tag="VEP73_10820"
     CDS             complement(944..2704)
                     /locus_tag="VEP73_10820"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HYT26962.1"
                     /translation="MRRPAVVALLACLWLVATGPAAGAHALLRSSDPASGASLDRAPQ
                     AVTVAFTEVPDPRLSIVHVLDTSGRSVEAGKAAAVPGRPLELRVPLGDLPRGTYTVSW
                     RTLSKSDGHVTAGAFAFGVGVQPGTVPAVQPGAARSTPPPNPLGVAGRWALYWGLALL
                     VGGAATGLAVFGGRLPERPRPLLAAGVGLAVSGLAAMTVAELADVGVPFRELLASGAG
                     HGLAREAAALAVTVAAVALLAGRPRSTVLLAVAAAAAAATMVVHVTTGHAAGASSLRP
                     LNLLVQSVHLVAAGVWVGGLAWLLAGLRGGDRPGRTAAVRRFSAMALTAVGVVAVTGL
                     LRAWQETGSWGRLLSTGFGRVLDVKVLLFAGLLAFGAVNRRRIVPALAAGAAGPAPTG
                     PPAQLGALRRNVRAEIGLATLVLLAAGLLTGLPPARSVATAARPAPPRVVEVSGNDYG
                     TTVLVDLVVTPGTAGPNAFRAKVVDYDSRAPFPASRVQLQFALPARPDLGASTLELAR
                     AGDGLWTGQGSPLSIDGRWRVTTLVQGAGTAVTVPLDLVTRQPDVAPPTTGQVQVQRV
                     PGQPTVYTITIAGGRTSRLM"
     gene            complement(2707..>2967)
                     /locus_tag="VEP73_10825"
     CDS             complement(2707..>2967)
                     /locus_tag="VEP73_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26963.1"
                     /translation="PVKDPTTGQLAQRLDREVPRLDSALDASRAAEKQARDDASSART
                     LAVIGIALGVLGLAVGGIAIGVARRSSSAASQPQDQVPAGRP"
BASE COUNT          363 a         1119 c         1195 g          290 t
ORIGIN      
        1 cctcgggcca ggcgccgtcc ccgcccgcgc ccatggcggc ggccgccgcg agcgcgaggt
       61 acccggcggc gagcacggcg gccgccccgg gcagctccca gagggccggc cgcaggagca
      121 ggaaggcgac cccggccgcg accacgccga gccgcgcgcc gacgcccggc gcccccgggc
      181 gggcgcgctc cgcgacgtgc cccgggccgg ccggggctgg acgtgcccga gtcaccgctc
      241 gacctggaac gccgaccggg tggacacctg ggggtcgaag ggcaggtggt cggacccgac
      301 gaagtcgacc cggaccacgt gctggccggg cggcaacgcc gggagccggc cggtcaggcg
      361 gtagttcatg gcgaccagcc tcccgtccac gaggacgtgc acgtggccct ggtcgggccg
      421 cacgcgcgtc gaggtgacgt tgacgatcct ggcgttgtcg agcctgagcc ggagcgtgat
      481 cgcgcccccg cgcaccttct gcccgttgcg gggcgagagg atggcgagct tggccgggct
      541 gctcgggcgc gcccccaggg tcgccggcgg cgaggtggcc gggggcttcg cctcgctctt
      601 gccgcagccc gccaggcagg cggctgccag ggccaggacg acggcgacgg tggcgccggg
      661 ccgtccgctc cgcatgaggc tcctcacttt ccgatcgcct gctcgaagcg cgcggtcgag
      721 gcggtcccgt cccgagccgt cgcggcgatg tcgaaggtcc agcgcccggg gccgagctcg
      781 ccctgggcgg cgacgtgccc ggggctgaac cgcaggaccc tgagggccgg ggcgcggccc
      841 gacggcgggg tcgcggtcac cttgacgtcc gcctgcggcg cttcgttgcc ccgcgcatcg
      901 aagaacgtga cgtggatctg gttggttccc ggcttccctg gtgtcacata agcctggagg
      961 tacgacctcc cgcaatcgtg atcgtgtaga cggtgggctg gccggggacc cgctgcacct
     1021 gcacctgccc ggtcgtcggg ggcgcgacgt cgggctggcg cgtcaccagg tcgagcggga
     1081 cggtgacggc ggtcccggcg ccctggacga gcgtggtgac gcgccagcgc ccgtcgatcg
     1141 acagggggct cccctgcccg gtccagagcc cgtcgccggc ccgggccagc tcgagcgttg
     1201 acgcgccgag gtcgggacgg gccggcaggg cgaactggag ctgcacccgg ctggcgggga
     1261 agggcgcccg cgagtcgtag tcgaccacct tcgcgcggaa ggcgttcggg ccggcggtcc
     1321 ccggggtgac gaccaggtcc accaggacgg tcgtcccgta gtcgttgccg ctgacctcca
     1381 cgacccgggg cggggccggc cgggccgcgg tcgccacgga gcgggccggc ggcaggccgg
     1441 tcagcaggcc ggccgcgagc agcaccaggg tggccaggcc gatctcggcc cggacgttcc
     1501 gccgcagcgc accgagctgc gcgggcggtc cggtgggagc gggcccggcg gccccggccg
     1561 cgagggcggg gacgatgcgg cggcggttca ccgcgccgaa cgccagcagg cccgcgaaca
     1621 gcagcacctt gacgtcgagg acgcgcccga agccggtcga gagcaggcgg ccccagctgc
     1681 ccgtctcctg ccaggcccgc agcaggccgg tcaccgccac cacgccgacg gcggtcaggg
     1741 ccatggcgga gaagcgccgc accgccgcgg tccgcccggg ccggtcgccg ccgcggaggc
     1801 cggcgagcag ccaggcgagg ccgccgaccc acaccccggc cgcgacgagg tggaccgact
     1861 ggacgagcag gttgagcggg cgcagcgacg aggcccccgc ggcgtggccg gtggtcacgt
     1921 gcacgaccat ggtggcggcg gcggccgccg ccgccacggc gaggagcacg gtcgagcgcg
     1981 gccggccggc gagcagcgcc accgcggcga ccgtgaccgc cagggcggcg gcctcgcggg
     2041 cgaggccatg gcctgcgccg gaggccagca gctcccggaa cggcacgccg acgtcggcca
     2101 gctcggcgac cgtcatggcg gcgagcccgg acaccgccag gccgaccccg gcggcgagca
     2161 gcgggcgggg gcgctccggg aggcggccgc cgaagacggc caggccggtc gccgcccccc
     2221 cgacgagcag ggcgagcccc cagtagagcg cccagcgccc ggccacgccg agcgggttcg
     2281 gcggcggcgt gctccgggcg gcacccggct gcaccgccgg gacggtgccc ggctggacgc
     2341 cgaccccgaa cgcgaacgcg cccgcggtga cgtgcccgtc ggacttcgac agcgtccgcc
     2401 agctcaccgt gtaggtgcct ctcggcaggt cgccgagggg caccctgagc tcgagcggcc
     2461 tgccagggac cgcggcggcc ttgccggcct ccaccgaccg gccggacgtg tcgagcacgt
     2521 ggacgatcga caggcgaggg tcgggcacct cggtgaacgc cacggtgacg gcctgcggcg
     2581 cccggtcgag cgaggccccg ctcgccgggt cggagctgcg caggagcgcg tgcgcgccgg
     2641 ccgccggccc ggtggcgacg agccacaggc aggcgagcag ggcgacgacg gccgggcgcc
     2701 gcaaggtcag ggcctccccg cagggacctg gtcctgcggc tggctcgccg cggacgacga
     2761 cctcctcgcg acgccgatgg cgatcccgcc gacggcgagg ccgagcacgc cgagcgcgat
     2821 cccgatcacc gcgagggtcc gcgccgagct ggcgtcgtca cgggcctgct tctcggcggc
     2881 ccggctcgcg tcgagagcgc tgtccaggcg cggcacctcg cggtcgaggc gctgggcgag
     2941 ctgcccggtg gtcgggtcct tgaccgg
//
LOCUS       DASPYY010000688         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1377360, whole genome shotgun sequence.
ACCESSION   DASPYY010000688 DASPYY010000000
VERSION     DASPYY010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377360"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VEP73_10830"
     CDS             complement(<1..342)
                     /locus_tag="VEP73_10830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT26964.1"
                     /translation="MQDFVTIGSEVAGYRVESFIGRGGMAVVYRAEDLRLGRRVALKL
                     LAPELSQNERFRQRFMRESQLAASIDHPNIIPIYEAGEADGLLYIVMRYVEGHDLKAL
                     VERDGPLDPART"
     gene            complement(343..1872)
                     /locus_tag="VEP73_10835"
     CDS             complement(343..1872)
                     /locus_tag="VEP73_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYT26965.1"
                     /translation="MLRARVLHLVVLLTATVALPFAVGAAPAAAHKHPGAPVVAEPAI
                     VYVQTSVLTKVSVVERSAGSSVTTPDQQTFSLGGGSGFVVNPDGTVVTVGRAIGNGGA
                     DVDTAVGDFEKQYGALKVPPDFVRTGFQDALGPTPHLRHGCFVTDASHSACVDFASPV
                     VHVFPYVSNPSRDGLKATVLEPGPGGGDVAVLKVESRDGMPTVNLASSSATAVPFTVL
                     GFTRPVTGQDRPATVVGHFKTGPGSRTIDPKQTKVGDLLARLGDGLAGGPLVNDEGRV
                     IAIASRAGDQVDFKFVEAIRDRLGAAGVQPAQGPVDTTFAEAKGYFDAQHYTPAVTRL
                     QEVLRLYPQHALAKSLLTVAVAKAGGPEDLHLREMQTMAAGGGRSPRFWLAVAAGALL
                     LVGLVLAVVFRNRLRRRKPPAAPGRAAGRAPAAASKSVGAFPASKSAPQPQAGRAGPP
                     ADGVVTGTPVPAGSRSQGQMQVRMARDLSGAKPGFCTQCGKPLAAGHRFCGFCGTRVM
                     A"
BASE COUNT          264 a          727 c          738 g          281 t
ORIGIN      
        1 cgtgcgggcc gggtcgagcg gcccgtcccg ttccacgagc gccttgaggt cgtggccttc
       61 gacgtacctc atgacgatgt agagcagccc gtcggcctcc ccggcctcgt agatggggat
      121 gatgttgggg tggtcgatgg acgcggcgag ctgcgactcg cgcatgaacc gctgccggaa
      181 acgctcgttc tgcgacagct cgggggccag cagcttcagg gcgaccctgc ggcccaggcg
      241 gaggtcctcg gcgcggtaga ccaccgccat gccgccgcgt ccgatgaacg actcgacgcg
      301 gtagccggcg acctcggaac cgatcgtgac gaagtcctgc atctacgcca tcacccgggt
      361 accgcagaac ccgcagaacc ggtggccggc ggccaggggc ttgccgcact gcgtgcagaa
      421 gcccggcttg gctccgctca ggtcacgggc catcctgacc tgcatctggc cctggctccg
      481 gctcccggcg gggacggggg tcccggtgac cacaccgtcg gccggcggcc cggcccgccc
      541 tgcctgcggc tgcggcgcgc tcttcgaggc cgggaaggcg ccgacgctct tggaggccgc
      601 ggcgggcgcc cggcccgccg cccggccggg ggccgccggc ggcttccgcc gccggagccg
      661 gttgcggaac accacggcca gcaccaggcc gacgagcagc agcgcgcctg ccgccacggc
      721 cagccagaag cgcggcgacc tcccgccgcc cgcggccatg gtctgcatct cccgcaggtg
      781 gagatcctcg gggccgccgg ccttggcgac cgcgacggtg aggaggctct tcgccagggc
      841 gtgctgcggg tagaggcgca ggacctcctg caggcgggtc acggccgggg tgtagtgctg
      901 ggcgtcgaag tagcccttgg cctccgcgaa cgtcgtgtcc accggcccct gcgcgggctg
      961 cacgcccgcg gcgccgagcc ggtccctgat cgcctccacg aacttgaagt cgacctggtc
     1021 gccggccctg gaggcgatgg cgatcacgcg gccctcgtcg ttgacgagcg ggccgccggc
     1081 caggccgtcg ccgagcctgg cgagcaggtc gccgaccttc gtctgcttgg ggtcgatggt
     1141 ccggctcccc ggtccggtct tgaagtggcc cacgacggtc gcgggccggt cctggcccgt
     1201 gaccggccgc gtgaacccga ggacggtgaa cggcaccgcg gtggcggagg agctggccag
     1261 gttgacggtg ggcatgccgt ccctggactc caccttcagg acggccacgt cgccaccgcc
     1321 cgggccgggc tcgaggaccg tcgccttgag gccatccctg ctcgggttcg agacgtacgg
     1381 gaagacgtgg accaccgggc tcgcgaagtc gacgcaggcg gagtggctcg cgtccgtcac
     1441 gaagcagccg tgcctgaggt gcggagtcgg cccgagcgcg tcctggaacc ccgtcctgac
     1501 gaagtccggc gggaccttca gggcgccgta ctgcttctcg aagtccccca cggccgtgtc
     1561 gacgtcggcc ccgccgttcc cgatggccct gccgacggtg acgaccgtcc cgtcggggtt
     1621 gaccacgaac ccgctcccgc ccccgagcga gaacgtctgc tggtcgggcg ttgtgacgct
     1681 gctcccggcg ctgcgctcga ccacgctgac cttggtgagg acggaggtct gcacgtacac
     1741 gatcgcgggc tcggcgacga cgggcgcgcc ggggtgcttg tgggcggcgg ccggggccgc
     1801 gcccacggcg aagggcaggg cgaccgtcgc ggtcaggagg acgacgaggt ggagaacgcg
     1861 cgcacggagc atcgatacct cccctgtctc cacctgaccg tcaggatgga gacgacggcg
     1921 accggccgcg tgtaccgaca gccggaccca tcgatccgcg tgacccgcct ggtccgatga
     1981 cctgcctggt ccgctttggt taatcttccg
//
LOCUS       DASPYY010000689         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2079312, whole genome shotgun sequence.
ACCESSION   DASPYY010000689 DASPYY010000000
VERSION     DASPYY010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079312"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VEP73_10840"
     CDS             <1..1224
                     /locus_tag="VEP73_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26966.1"
                     /translation="EDMQGGYVQLTRGKERTDMYLTVGPEPLADEAHPHPRGEPVTPE
                     ALLGRVLTRDGAKTLATDTPVLLDVRRLSTRQLREERDRLAELRAECPADRARELHLA
                     RQRAAELDAARRTVVSEREAAAAEVAQVGGSVWHRREAAAARERLALAEHAVRATGRQ
                     AAQAAERAGRLRRAEAERTGWLEQHADLPAQERATARELAWRKRVDARAVALEQPGWL
                     LAELGPMPPAEQLAGRAAWLAAAVELDTWRRTHGLDDPRPAEQGRGQRARPGRAVAPA
                     AARQGRRPTPERPAPAGEGRGGDQVDQTGAPRESRRGGPRWRHPGRERPAHPDAPTGE
                     GRSATAEALLGAEPGRSEPGRRRDWQQARAALDRLAAHRERTRDPDHHRAGRDDRHRD
                     HRRPERAAPHERGER"
     gene            1247..2272
                     /locus_tag="VEP73_10845"
     CDS             1247..2272
                     /locus_tag="VEP73_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYT26967.1"
                     /translation="MHQPRPYRPDDFDDLEPDDFDDGEGFGTVVYEGTGLTVRALTAE
                     QVDRFLDAHTGEPARLLGGGWDQRPPLPPPPVGDAPDSSGAAGAGWPAPVLADPTRTP
                     AGGYGTPGHSALAMYRRERARELAAWSCSLPWRAALVLAAAAGAAILAGAAGLHGAAL
                     LLAGLVAGGVVGWRLRFRVSKDTRAWRDGARGERATARLLRRLARHGAAVFHDVAIPG
                     IPANADHVVIGPGGVVLVDSKRYTGRVWQDPGGRVWHNRYPMDRPLRALRMETAAIAH
                     LLGVAVRPVVCVHGAHVDFAGLTAADIDIVPAGRLASVLTASGQRLSRADVRALVSHA
                     HTMLRPA"
     gene            2697..3197
                     /locus_tag="VEP73_10850"
     CDS             2697..3197
                     /locus_tag="VEP73_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HYT26968.1"
                     /translation="MGCQEGTAVVETTQDGTLRGVHWWPPPGAFDLDPAVPETIRGAY
                     DEGMHCLGAQCPRAAAVMFRRVLEALVNDKGSAAVQAAAAKNLAQGLKVMAAEHVLQP
                     TLAEWATEIRVVGNVGAHLDPIKDVALDEARDLARLTRQLLEYVYELPARVARSRQAR
                     QAVPTP"
BASE COUNT          408 a         1281 c         1252 g          363 t
ORIGIN      
        1 gaggacatgc agggcggcta cgtgcagctg acccgcggca aggagcgcac cgacatgtac
       61 ctgaccgtcg gccccgaacc cctcgccgac gaggcccatc cccacccgcg tggtgagccg
      121 gtcacaccgg aggcgctgct cggtcgggtg ttgacccgcg acggcgccaa gaccctggcc
      181 acagacacgc cggttctgct ggatgtgcgc cggctgtcga ccaggcagct gcgcgaggag
      241 cgcgaccggc tggccgagct gcgcgccgag tgccccgcgg accgcgcccg ggagctgcac
      301 ctggcccggc agcgcgccgc cgagctggac gccgcccgcc gtaccgtcgt aagcgagcgg
      361 gaggccgcgg cggccgaggt ggcccaggtc ggggggagcg tgtggcaccg ccgggaggcc
      421 gctgcggcca gggagcggct ggcgctggcg gagcatgcgg tgcgggccac cggccggcag
      481 gccgcgcagg ccgccgagcg ggccgggcgg ctccgccgcg ccgaggccga gcgcaccggc
      541 tggctggagc agcacgccga cctgcccgcc caggagcggg ctactgctcg ggagctggcg
      601 tggcgcaagc gggtggacgc ccgcgcggtc gcgctcgagc agcccggctg gctgctcgcc
      661 gagctcggcc ccatgccccc ggccgagcag ctcgccgggc gggcggcgtg gctggccgcc
      721 gcggtggagc tggacacctg gcggcgcacc cacggcctcg acgacccaag gccagctgag
      781 caggggcggg ggcagcgtgc ccggccgggg cgggccgttg cgccggccgc cgcacgccaa
      841 ggcaggaggc cgaccccgga gcggcccgcc ccggccggcg agggacgcgg cggcgaccag
      901 gtcgaccaga ccggcgcgcc cagggagtcg cggcggggcg ggccgcgctg gcgccacccc
      961 ggccgggagc ggcccgccca ccccgacgcc cccaccggcg aggggcgctc ggcgaccgcc
     1021 gaggcgctgc tcggcgccga gcccggccgc agcgaacccg gccggcgccg cgactggcag
     1081 caggcccgcg ccgcgctcga ccggctcgcc gcccaccggg aacgcacccg cgaccccgac
     1141 caccaccgcg ccggtcgcga cgaccggcac cgcgaccacc gccgccccga gcgcgctgcc
     1201 ccgcacgagc gcggcgagcg gtagcccgca ccatagggag cccgccatgc accagcccag
     1261 gccctaccgg cccgacgact ttgacgacct cgagcccgac gacttcgacg acggtgaggg
     1321 gtttggcacg gtggtctatg aggggaccgg gctgaccgtc cgggcgctca ccgccgagca
     1381 ggtcgaccgg ttcctcgacg cccacaccgg cgagcccgcc cggctgctcg gcggcggctg
     1441 ggaccagcgc ccacccctgc ccccgccgcc cgtgggggat gcaccggact cctccggcgc
     1501 ggctggggct ggctggccgg cgcctgtgct ggccgacccg acccgtaccc ccgcgggcgg
     1561 ctacggcacc cccggccact cggcccttgc gatgtatcgg cgcgagcggg cccgcgagct
     1621 ggccgcctgg tcctgctcgc tgccctggcg cgccgccctg gtgctggccg ccgccgcggg
     1681 cgcagcgatc ctggccgggg cggccggcct gcacggcgct gccctgctgc ttgccgggct
     1741 ggtcgctggg ggcgtggtcg gctggcggct gcgcttccgg gtctcgaagg acacccgggc
     1801 gtggcgcgac ggcgcccgcg gggagcgggc caccgcccgc ctgctccgcc ggctcgcccg
     1861 ccacggcgcc gcggtcttcc atgacgtggc catccccggc atccccgcta acgccgacca
     1921 cgtcgtgatc ggccccggcg gggtggtgct ggtggattcc aagcgctaca ccggccgcgt
     1981 ctggcaggac ccgggcgggc gggtctggca caaccggtac cccatggacc gccccctgcg
     2041 cgccctgcgc atggagaccg ccgcgatcgc ccacctgctc ggggtggcgg tgcgccctgt
     2101 ggtgtgcgtc cacggcgccc acgtcgactt cgccggcctc accgccgcag acatcgacat
     2161 cgtgcccgcg ggccggctcg ccagcgtgct caccgcctcg gggcagcggc tcagcagggc
     2221 cgacgtgcgt gcgctggtga gccacgcgca caccatgctc cgccctgcct gatggctcgc
     2281 ccgcggccac gggggcggcg ccgggcggcc gggtagcctg gtacctcacc ctgcccacgg
     2341 ggaggaccac ggccgccggg cgagcttggc ccaacggcca tggggagcat ctcgccccgg
     2401 ggcgggtcgg gtcgctgacc gtgagcaccg gtatacttac accgacgtaa ctccgaccgg
     2461 tccgagcgtt tcccccagtc gcccggcagt cgggccccag gccacctcag aggcgagaca
     2521 agcgcgatgg cgaaccccga cacgctgcca gactcctccg acccgacccg accgggccct
     2581 gcccgcgctg cggccggacc tccaactttc agctcggagg cgccttcacg accaagaccg
     2641 gtgatgttcc tggggccggc ctggccgtga ttgagcgcgt cgtggtgatg acctgcatgg
     2701 gttgtcagga aggcacggcc gtggtcgaga cgacccagga cgggacgttg cggggtgtgc
     2761 actggtggcc gccgccgggc gcgttcgatc tggacccggc cgttcccgag accatccgcg
     2821 gtgcctacga cgaggggatg cactgtctcg gcgcgcagtg cccgagggca gcggcggtga
     2881 tgtttcgtcg cgtgctcgag gcgctcgtca acgacaaggg cagcgccgcc gtgcaggccg
     2941 cagccgccaa gaacctcgcc caagggctca aggtcatggc cgccgagcat gtcttgcagc
     3001 ctaccctcgc cgagtgggca accgagatcc gcgtggtcgg caacgtcggt gcgcatctgg
     3061 acccgatcaa ggacgtggcg ctcgacgagg cgcgggacct ggcccggttg actcgccagt
     3121 tgctggagta cgtgtacgag ctgcccgccc gggtcgctcg cagccggcaa gctcgtcagg
     3181 cggtcccgac cccgtagcca ggacgtcgtg cgggtggccg cgagacccgt cccggcggga
     3241 cggcgccgca gccgggtcgc ctggtgccgc agggatccag cagcatccag agggggaggc
     3301 gccg
//
LOCUS       DASPYY010000690         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2219743, whole genome shotgun sequence.
ACCESSION   DASPYY010000690 DASPYY010000000
VERSION     DASPYY010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219743"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..>1899)
                     /locus_tag="VEP73_10855"
     CDS             complement(484..>1899)
                     /locus_tag="VEP73_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26969.1"
                     /translation="QTVVILTRLRSEADQFNTACQQLREQAGQLGTERVQVRDRSFAV
                     GDLVVCGRNALQPLGVANATRGRITALDAEQRSITLALHDGTQVTLPRAYLDERPGWW
                     TRGNPDRRTLDLAYATTGHKAQGLTVERALVRVTGAEDGNWLTVQTSRAKRETMLYAV
                     VGPEPRTPELDEPPPPARTVAEQLTAGLRRDGAERLATDTAVQLDLRRMSTAELRAER
                     TRLAHLLRDAPPDRARLLAHTTRLREQAEQGWGQANHQAEAARQRAADLEHGPGRLLH
                     RGELAQAREHAALTETAAQLARGQAERAADRQRDARRGQQVHLAWREDHAEDLVAERA
                     TTRELAWQTRVAATAAELDRPAWIAELGEPPTSLRGRRAYRDATRLLSDYRDRYQVTD
                     PDRALGPEPRGGDLEQRRAHRSCREAIERVQGKQRAERDRTDRADPGERDPDQRAQQA
                     RRERSHQRDRAGREGRERDAG"
BASE COUNT          180 a          676 c          763 g          280 t
ORIGIN      
        1 tgaccggccg gttcggttgt gcctggtcat cggcgcggcg ggctcgctcc ggcttcgtca
       61 tcaccgggag tgtcgccgca tcaccaaata gcgtgtcgcg taacagtagg gggagcgtgg
      121 cgctcgtggg cgctcaccat cagcggacgt cggctaccat gcagacaccg acggccggac
      181 gcggcggtgt ccactgcgga cgctccgcct gtccggcgtg gacaccggtc ggcggctgcg
      241 gcgtgggagc ggacgctgcg ggcatccacg gtgtccatct ggccgtggcg gctcggacgg
      301 tccggagact gcggccagga ctggacggac agccggtgcg gacaccgcgt aggcagtgga
      361 cacggcggca cagtccggct ggatcgggcg agacacgccg attgacggct aagtgtcaat
      421 gccagcccgg gtgatcacgg ccccgctcat gctcgcgtcg cgccggtgcg cgctcacgag
      481 cgctcagccg gcgtcacgct cgcgcccctc gcgcccggcg cggtcgcgct ggtgcgagcg
      541 ctcccggcgg gcctgctgcg cccgctggtc ggggtcgcgc tcgccggggt ccgcgcggtc
      601 ggtccggtcg cgctcggcgc gctgcttgcc ctgcacccgc tcaatcgcct cccggcagct
      661 ccggtgggcg cgccgttgct ccaggtcgcc ccctcgcggc tcggggccga gcgcccggtc
      721 ggggtcggtg acctggtagc ggtcgcggta gtcgctgagc aggcgggtgg cgtctcggta
      781 ggcccgccgg ccgcgcaggc tggtgggcgg ctcgccaagc tcggcaatcc aggccgggcg
      841 gtccagctcg gcggccgtgg cggccacccg cgtctgccag gcaagctccc gggtggtcgc
      901 ccgctcggcc acaaggtcct cggcgtggtc ctcccgccac gccaggtgga cctgctggcc
      961 gcggcgggcg tcgcgctgcc ggtcggcggc acgctcggcc tgcccgcgtg cgagctgcgc
     1021 ggcggtctcg gtgagggcgg cgtgctcgcg ggcctgggcg agctcgccgc ggtgcagcag
     1081 ccgccccggg ccgtgctcga ggtcggcggc gcgctggcgg gccgcctcgg cctggtggtt
     1141 ggcctgcccc cagccctgct ctgcctgctc gcggagccgg gtggtgtggg ccagcagccg
     1201 cgcccggtcc ggcggcgcgt cccggagcag gtgggcgagg cgggtgcgct cggcgcgcag
     1261 ctcggccgtg ctcatgcggc gcaggtccag ctggacggcc gtgtcggtcg ccagccgctc
     1321 ggcaccgtcg cggcgcaatc cagccgtgag ctgctcggcc acggtccgtg ccgggggtgg
     1381 gggttcgtcc agctcggggg tgcgcggctc ggggccgacg actgcgtaca gcatcgtctc
     1441 gcgcttggcg cgggaggtct gcacggtgag ccagttgccg tcctcggcgc cggtcacccg
     1501 caccagggcg cgctccacgg tgagcccctg ggccttgtgg ccggtggtgg cgtaggccag
     1561 gtcgagggtg cgccggtcgg ggttgcccct ggtccaccag ccgggccgct cgtcgaggta
     1621 ggcgcggggg agggtgacct gcgtgccgtc gtgcagcgcc agcgtgatcg accgctgctc
     1681 ggcgtcgagg gcggtgatgc gcccgcgggt ggcgttggcg accccgaggg gttgcagggc
     1741 gttcctgccg cacaccacca ggtcccccac ggcgaacgat cggtcgcgga cctgcacgcg
     1801 ttcggtgccg agctgcccgg cctgctcgcg gagctgctgg caggcggtgt tgaactggtc
     1861 ggcctcactg cgcagccggg tcaggatcac caccgtctg
//
LOCUS       DASPYY010000691         5738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2781744, whole genome shotgun sequence.
ACCESSION   DASPYY010000691 DASPYY010000000
VERSION     DASPYY010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781744"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VEP73_10860"
     CDS             <1..549
                     /locus_tag="VEP73_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26970.1"
                     /translation="AFASALTGVAAAVVLAGMALGATAVLLLAAGVCLAILPLATGAA
                     ILRYRLYDLDRIISRTLAYGLLTVLLGGGYAGIVLGLGQLLGRRSSLAVAVATLALAG
                     AFQPARRRIQGLVDRRFNRRRYDAAQTIQAFSARLRQQVDLDTLTAELLGVVEQTMQP
                     ASVSLWLRPSVSAAPSPSAPNP"
     gene            629..1399
                     /locus_tag="VEP73_10865"
     CDS             629..1399
                     /locus_tag="VEP73_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HYT26971.1"
                     /translation="MRRSKDVNELIDRMQALFLPLEERGDPGRYFLATYQRTTRAVQA
                     ELAAGGFLDPAWVERWDLAFADFYLEALETVEAGRRPSEPWAVAFGAAARDGFPPLRH
                     VLLGMNAHINYDLAQSLLAVITDEELDDPELVARRAADHRHIDEVLAARVGSEDAELE
                     ALSGARSRTDRLLAPLNRLGTRRFLKESREKVWANTRLLSLARRAGPAPYAERLAELE
                     RLAAARVADLVAPGQVVLKLAVKGFGVRLPDTPGPPSG"
     gene            complement(1401..2720)
                     /locus_tag="VEP73_10870"
     CDS             complement(1401..2720)
                     /locus_tag="VEP73_10870"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HYT26972.1"
                     /translation="MAQLWKGETEKAIRNFPISGERVPAQVVHMLARIKAEAARVNAE
                     LGGLDADRAERIAAAAESVAAGEHDDQFPIDVFQTGSGTSTNMNVNEVVASLAGDGVH
                     PNDHVNMGQSSNDAFPTAVHLAALHAASADLVPALRSLERSLEAKAAEFADVVKPGRT
                     HLMDAVPVTLGQELAGYAAQVREGADRVEGTLARVGKLPLGGTAVGTGLNAHPEFAAR
                     VRERLAAATGLALSPPAHPFEAQGARDALVELSGALKVVAVSCTKIANDIRWMGSGPR
                     AGLGEIFLPELQKGSSIMPGKVNPVLAEVLAQVSAQVIGNDTAITVGGLSGAFELNVY
                     VPLLARNLLQSITYLAAACREFAARCVDGIEANRARAQELVESTLVTVTALNPLVGYD
                     EAAAIAKEAAASGRSLREVALERGVDADALDKALDLRAMAHGNGFAG"
     gene            complement(3304..>5738)
                     /locus_tag="VEP73_10875"
     CDS             complement(3304..>5738)
                     /locus_tag="VEP73_10875"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HYT26973.1"
                     /translation="EERQRVRTLRERGQGAGPVSESFQAAVGEVRASEGEGGLAALLE
                     RLEVTPVLTAHPTEARRRAVVDALLRIGAQVDRLDDPRLSASERADVQRRLLEEITVL
                     WRTEQLRRERPTPLDEVRTVMSVFDETVFRLVPTVYRQLERAIAGDAAGTGPPPLRPF
                     LRWGSWVGGDRDGNPNVTAEVTRAAMGIQADHVLRGLEAGTRRVGRSLTVSAGSTPPS
                     PELAASLERDEAAFPLLAAAARARSPDEPHRRKLVLVAERLAVTRLALAVPAERARGG
                     YADAAQLLADLRLVQGSLAAAGAGRLAYGELQHLVWQAETFGFHLASLEVRQHASVHA
                     EVLAELAPGAVGDPAALDRLARDGWPAGVADRSETAAEVLATLQTMAELQACYGADAC
                     RRYVVSFTRSAADLAAVRALGRLAVPDGSPALDVVPLFESRADLEAAPEVLDAWVALP
                     GTTAWLEARGRRLEVMLGYSDSAKDAGFLAANLALYRAQGALAAWAARNAVDLTLFHG
                     RGGALGRGGGPASRAVRGQAPGSVAGRFKVTEQGEVIFARYGNPAIGRRHLEQVTNAV
                     LVASTQRAQAALAESQERFAAAAALMARASEAAYRDLVGREGFAEFFAKVSPLEELGD
                     LRIGSRPARRDARRGLESLRAIPWAFAWSQNRCNLPGWYGLGAGLAAVAGEPGGRETL
                     AAMYAGWPFFTSLIENAEMSLAKADPLIAELYLEFGERPDLSAAIHEELARTRALVGA
                     VTGSDRLLDRRPVLRRAVDLRNPYVDALSFLQVRFLAELRAGPDARDRDRVANLVLLT
                     VNGVAAGLQNTG"
BASE COUNT          736 a         2124 c         2138 g          740 t
ORIGIN      
        1 gcattcgcct ccgccctgac cggggtggcg gcggcggtcg tcctggccgg catggcgctg
       61 ggcgccacgg cggtgctcct gctggcggcc ggcgtgtgcc tggcgatcct gccgctggcg
      121 acaggcgcgg cgatcctgcg ctaccggctg tacgacctgg accgcatcat cagccgcacg
      181 ctcgcctacg gcctgctcac ggtactgctg ggcggcggct acgccgggat cgtcctggga
      241 ctcgggcagc tgcttggccg acgctcgagc ctggcggtgg cggtggcgac cctggcgctg
      301 gccggggcgt tccagccggc ccgccgccgc atccaagggc tggtggaccg gcgcttcaac
      361 cggcgccgct acgacgccgc ccagacgatc caggcgttca gcgcgcggct gcgccagcag
      421 gtcgacctgg acaccctgac cgccgagctg cttggcgtag tcgagcagac catgcagccg
      481 gccagcgtgt cgctgtggct tcgaccctcg gtcagcgcgg caccttcccc gtccgctccc
      541 aatccgtgaa cacgctcatc gcatccccct cggacgcccg gccccagagg ctggacgccg
      601 gcctgggttg ctgccaggct tccgctccat gcgacgatcc aaggatgtca acgagctgat
      661 cgaccggatg caggcgctgt tcctcccgct ggaggaacgc ggcgacccgg gcaggtactt
      721 cctggccacc taccagcgga ccaccagggc ggtccaggcc gagctggcgg cgggcgggtt
      781 cctcgacccc gcctgggtcg agcgctggga cctggcgttc gccgacttct acctcgaggc
      841 gctcgagacc gtcgaggcgg ggcggcgccc gtccgagccc tgggcggtcg cgttcggggc
      901 ggccgcccgg gacggcttcc cgccactgcg ccacgtgctg ctcggcatga acgcccacat
      961 caactacgac ctcgcccagt cgctgctcgc ggtcatcacc gacgaggagc tcgacgaccc
     1021 cgagctggtc gccaggcggg cggccgacca ccggcacatc gacgaggtgc tcgccgcccg
     1081 ggtcgggtcc gaggacgccg agctcgaggc gctcagcggt gcccgcagcc gcaccgaccg
     1141 gctgctcgcg ccgctcaacc ggctcgggac caggcgcttc ctcaaggagt cgcgtgagaa
     1201 ggtctgggcc aacacccgcc tgctcagcct ggcaaggcgc gcagggcccg cgccgtacgc
     1261 cgagcggctg gccgagctgg agcgcctggc ggcggccagg gtcgccgacc tcgtcgcccc
     1321 gggccaggtc gtgctgaagc tggccgtcaa gggcttcggg gtgcggctcc cagacacccc
     1381 tggcccgccc tccggctgag ctagcccgcg aagccgttgc cgtgcgccat ggcgcgcagg
     1441 tcgagggcct tgtcgagcgc gtcggcgtcg acgccccgct cgagggcgac ctcgcgaagc
     1501 gaccggcccg atgcggccgc ctccttggcg atcgcggccg cctcgtcgta gccgacgagc
     1561 gggttgagcg cggtgacggt caccagcgtg gactcgacca gctcctgggc acgcgcgcgg
     1621 ttggcctcga tgccgtcgac gcacctggcg gcgaactccc ggcacgcggc ggccaggtag
     1681 gtgatcgact ggagcaggtt ccgggccagc agcgggacgt agacgttcag ctcgaacgct
     1741 ccggacaggc cgccgaccgt gatcgccgtg tcgttgccga tcacctgggc cgacacctgg
     1801 gccaggacct cggcgaggac cgggttcacc ttcccgggca tgatcgagga gcccttctgc
     1861 agctcgggca ggaagatctc gcccagcccg gctcttgggc ccgaccccat ccagcggatg
     1921 tcgttggcga tcttcgtgca gctcaccgcc acgaccttga gcgccccgct gagctcgacg
     1981 agtgcgtcgc gggcgccctg tgcctcgaac gggtgggccg gcggcgagag cgcgagcccg
     2041 gtggcggcgg cgagccgttc ccggacccgg gcggcgaact cggggtgggc gttcaagccg
     2101 gtgccgaccg cggtgccccc gaggggcagc ttgccgaccc tggccaaggt cccctcgacg
     2161 cggtcggcgc cctcgcgcac ctgggcggcg tagccggcga gctcctgccc gagggtgacc
     2221 gggaccgcgt ccatcaggtg ggtgcgcccc ggcttgacca cgtcggcgaa ctcggcggcc
     2281 ttggcctcga gggaccgctc gaggctgcgg agcgccggga cgaggtcggc gctcgcggcg
     2341 tgcagcgcgg cgaggtgcac ggcggtcggg aaggcgtcgt tggacgactg gcccatgttg
     2401 acgtggtcgt tggggtggac gccgtcgccc gcgaggcttg ccaccacctc gttcacgttc
     2461 atgttggtcg aggtgcccga gccggtctgg aacacgtcga tcgggaactg gtcgtcgtgc
     2521 tcaccggcgg ccaccgactc ggcggcggcg gcgatccgct cggcccggtc ggcgtccagg
     2581 ccgccgagct cggcgttgac gcgggcggcc tctgccttga tgcgcgcgag catgtggacg
     2641 acctgcgccg gcacgcgctc gccggagatg gggaagttcc ggatcgcctt ctcggtctcg
     2701 cccttccaga gctgcgccat cgtcggcctc ctcgtcccgc tcgatcaccc gcatgctact
     2761 ggcggggtcg ggcctccgca cccgccgggc ggccggacga ggaagaggcg gcacgtacca
     2821 cgtgccgcct cttcaaggga agtcaaggtc accggcctaa ggccgccggc cgggaccgcc
     2881 tgtgcccggc ccggctacga ccacgtcgtc gagttcgacg gcgaggccgg cgtcgatcga
     2941 tctgccatgc tcctcgattc ctcggctgcg ttgctggaga gcgccatttc ggcccgccgc
     3001 cgggggggag cggcggaccg aattatggcg tgcggcgccg ggtgactccg cactcggagg
     3061 acacggtgca gctcatgcgc ctcccttacg tctccaggcg atccggaggg gtccgccagg
     3121 ggggatgaga cgtccagtcg gcgtaggcca agctttgccc aaatcgatat gaaaatgcaa
     3181 atcgcaagga caacggggtg ttgtgcgaat gtgcaccaag gccaggacaa aaccagcgac
     3241 cgacgccctc ccgttccctt acgtatattt gacgagctcc cctctggaaa atgccgaagc
     3301 cggtcagccg gtgttctgca ggcccgcggc gacgccgttg accgtgagca ggacgagatt
     3361 cgcgacccgg tcccggtccc gagcgtccgg gcccgcccgc agctcggcga ggaagcggac
     3421 ctggaggaac gacagcgcgt cgacgtaggg gttgcggagg tcgacggcgc ggcgcagcac
     3481 cggccggcgg tcgagcaggc ggtcgctgcc ggtcaccgcg ccgacgagcg cgcgggtgcg
     3541 cgcgagctcc tcgtggatcg cggcggacag gtcggggcgc tcgccgaact cgaggtacag
     3601 ctccgcgatc agcgggtcgg ccttggccag gctcatctcc gcgttctcga tgagcgaggt
     3661 gaagaagggc caacccgcgt acatcgctgc gagcgtctcg cggccaccgg gctcgccggc
     3721 caccgcggcc aggccggcgc cgaggccgta ccatccgggc aggttgcagc ggttctggct
     3781 ccaggcgaac gcccagggga tggcccgcag ggactcgagg ccgcgccgtg cgtccctgcg
     3841 ggccggccgc gagccgatcc gcaggtcgcc gagctcctcc agcgggctca ccttggcgaa
     3901 gaactccgcg aagccctccc gcccgacgag gtcgcggtac gcggcctcgg acgctctggc
     3961 catcagcgcc gcggcggcgg cgaagcgctc ctggctctcg gcgagcgccg cctgcgcccg
     4021 ctgggtcgag gcgaccagga cggcgttcgt cacctgctcg agatggcggc ggccgatggc
     4081 ggggttgccg tagcgggcga agatcacctc gccctgctcg gtgaccttga accggccggc
     4141 gaccgagccg ggcgcctgcc cccgcacggc ccggctggcc gggccgcccc cgcgccccag
     4201 cgcgccgccc cggccgtgga acagcgtgag gtcgacggcg ttccgcgcgg cccaggccgc
     4261 gagcgcgccc tgggcacggt agagggcgag gttggcggcg aggaagccgg cgtccttggc
     4321 cgagtcggag tagccgagca tgacctcgag gcgccggccc cgcgcctcga gccaggcggt
     4381 cgtccccggc agcgcgaccc aggcgtcgag cacctcgggg gccgcctcca ggtcggcccg
     4441 ggactcgaac agcgggacga cgtcgagcgc cggcgacccg tcgggcacgg ccagccggcc
     4501 cagcgcccgg accgccgcga ggtcggcggc cgaccgggtg aagctgacca cgtagcgacg
     4561 gcaggcgtcc gcgccgtagc aggcctgcag ctcggccatc gtctgcaggg tcgcgagcac
     4621 ctcggccgcg gtctccgagc ggtcggcgac gccggccggc cagccgtcgc gcgcgagccg
     4681 gtcgagggcg gccgggtcgc cgaccgctcc gggcgcgagc tcggcgagca cctcggcgtg
     4741 cacggaggcg tgctggcgca cctcgagcga ggcgaggtgg aacccgaacg tctccgcctg
     4801 ccagacgagg tgctggagct ccccgtaggc gagccggccc gccccggccg cggccagcga
     4861 gccctggacg aggcggaggt cggcgaggag ctgggccgcg tccgcgtacc cgccgcgcgc
     4921 ccgctcggcg ggcaccgcga gcgcgagcct ggtcacggcc aggcgctctg ccacgaggac
     4981 gagcttgcgc cggtgcggct cgtcgggcga ccgggcgcgg gcggcggcgg cgagcagcgg
     5041 gaacgcggcc tcgtcgcgct cgagcgacgc ggccagctcc ggcgagggcg gggtggatcc
     5101 cgccgagacg gtcagcgagc ggccgacccg gcgcgtcccc gcctccaggc cgcggagcac
     5161 gtggtcggcc tggatcccca ttgccgcccg ggtgacctcg gcggtgacgt tggggttgcc
     5221 gtcccggtcg ccgcccaccc agctccccca ccgcaggaag gggcgcagcg gcggcgggcc
     5281 ggtgcccgcc gcgtccccgg cgatggcacg ctcgagctgc cggtacacgg tgggtaccag
     5341 gcggaacacc gtctcgtcga acacggacat gaccgtgcgc acctcgtcga gcggggtcgg
     5401 ccgctcacgc cggagctgct cggtgcgcca caggacggtg atctcctcga gcagccggcg
     5461 ctgcacgtcc gcccgctccg aggccgacag ccgggggtcg tccagccggt cgacctgggc
     5521 cccgatccgg agcagggcgt cgaccaccgc ccgccgcctg gcctcggtcg gatgggcggt
     5581 cagcaccggg gtcacctcga gccgttcgag cagcgcggcc agcccgccct cgccctccga
     5641 ggcgcgcacc tccccgaccg cggcctggaa cgactccgac accgggcccg ccccctggcc
     5701 gcgctcgcgc agggtgcgga cacgctgccg ctcctcgg
//
LOCUS       DASPYY010000692         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_955817, whole genome shotgun sequence.
ACCESSION   DASPYY010000692 DASPYY010000000
VERSION     DASPYY010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955817"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VEP73_10880"
     CDS             complement(<1..608)
                     /locus_tag="VEP73_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HYT26974.1"
                     /translation="MTATPADAEVAPTALVLPGVELGAGSRVGEFCIVGEPPGGAATA
                     TSKREALDGHPWSGPVTVIGEGALLRSHTVVYAGSRIGARFQTGHHVLVREAGEIGDD
                     VSVGTGSVVEHHVRIGHRVRLHTGTFVPEYTVLEDDCWIGPRVVLTNAPFPRCPDVAA
                     CMRGVRVERGAKLGANVTVLPGVVVGAGALVGAGAVVTRDVPP"
     gene            complement(589..>1651)
                     /locus_tag="VEP73_10885"
     CDS             complement(589..>1651)
                     /locus_tag="VEP73_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT26975.1"
                     /translation="RCQIDDIRVALLGFGYWGPNLARNLHLRLGRAWVACVDPDPARR
                     GEVAHRYPWVRVLADPAGVLADPGVDAVVIASPARTHAELAHRALAAGKHVMVEKPLA
                     LTTDGAIALAEEAGASGRVLMVGHTFEYNPAVTKMRALLEAGELGDLYYLHSQRVNLG
                     RIQQDVNALWSIGPHDVSIANYLVGTAPRWVSARGARYLGTDVEDVVFATLGYDGGVL
                     AHVHVSWLDPSKLRRTTMVGSRRMVVFDDLDSEAKLRVYDKGADPVGVGEFGEYQFRL
                     RSGDIHVPRTDLTEPLALELDHFLECVRTGATPRTDAWNGVRVVATLEAAQQSLDKGG
                     LQVGVAGTADEDRRDRHAR"
BASE COUNT          212 a          580 c          659 g          200 t
ORIGIN      
        1 gggggcacgt cgcgggtcac caccgccccg gcgccgacca gcgcgcccgc gccgacgacg
       61 acgccgggca ggacggtgac gttggcgccg agcttggcgc cgcgctcgac ccgcacgccg
      121 cgcatgcagg cggcgacgtc ggggcagcgg gggaaggggg cgttggtcag caccacccgc
      181 ggcccgatcc agcagtcgtc ctcgagcacg gtgtactcgg ggacgaacgt gccggtgtgg
      241 agccggaccc ggtggccgat gcggacgtgg tgctcgacga ccgagccggt gccgacggag
      301 acgtcgtcgc cgatctcgcc cgcctcccgg acgagcacgt ggtggccggt ctggaagcgg
      361 gcgccgatgc ggctgcccgc gtagacgacg gtgtgggagc ggagcagggc gccctcgccg
      421 atcacggtca ccggcccgga ccaggggtgg ccgtcgagcg cctcgcgctt ggacgtcgcg
      481 gtggccgcgc cgcccggcgg ctccccgacg atgcagaact cgccgacccg gctgcccgcg
      541 ccgagctcga cccccgggag gacgagggcg gtcggggcga cctcggcgtc agcgggcgtg
      601 gcggtcacgg cggtcctcgt cggcggtccc ggccacgccg acctgcagcc cgcccttgtc
      661 gagcgactgc tgggcggctt ccagggtcgc caccacccgg acgccgttcc aggcgtcggt
      721 gcggggggtg gcgccggtcc ggacgcactc gaggaagtgg tcgagctcga gggcgagcgg
      781 ctcggtcagg tcggtgcgcg gcacgtggat gtcgcccgag cggagccgga actggtactc
      841 accgaactcg ccgacgccga ccgggtcggc gcccttgtcg tagacccgga gcttcgcctc
      901 ggagtcgagg tcgtcgaaca cgaccatgcg ccgcgagccg accatcgtgg tgcggcgcag
      961 cttggacggg tcgagccagg acacgtggac gtgggcgagg acgccgccgt cgtagccgag
     1021 ggtggcgaac accacgtcct cgacgtcggt gccgaggtag cgggcgcccc gcgccgagac
     1081 ccagcggggc gccgtgccga ccaggtagtt cgcgatcgac acgtcgtggg gcccgatcga
     1141 ccagagcgcg ttgacgtcct gctggatgcg gcccaggttc acccgctggg agtgcaggta
     1201 gtacaggtcg ccgagctcgc cggcctcgag cagcgcgcgc atcttggtca cggccgggtt
     1261 gtactcgaag gtgtggccca ccatgaggac ccggccgctg gcgccggcct cctcggcgag
     1321 tgcgatcgcg ccgtcggtgg tgagcgccag cggcttctcg accatgacgt gcttgccggc
     1381 ggccagcgcc cggtgcgcga gctcggcgtg ggtgcgggcg ggcgaggcga tgacgaccgc
     1441 gtccacgccc gggtcggcga gcacgccggc cgggtcggcc aggacccgga cccaggggta
     1501 gcggtgggcg acctcgcccc ggcgggcggg gtcggggtcg acgcaggcga cccaggcccg
     1561 ccccaggcgg aggtggaggt tgcgcgccag gttcggcccc cagtacccga agccgagcag
     1621 ggcgaccctg atgtcgtcga tctggcacct c
//
LOCUS       DASPYY010000693         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2782150, whole genome shotgun sequence.
ACCESSION   DASPYY010000693 DASPYY010000000
VERSION     DASPYY010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782150"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VEP73_10890"
     CDS             complement(<1..104)
                     /locus_tag="VEP73_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT26976.1"
                     /translation="MPEAAADLVVVGGGPAGVAAALAGRRAGAEVVLL"
     gene            complement(97..1062)
                     /locus_tag="VEP73_10895"
     CDS             complement(97..1062)
                     /locus_tag="VEP73_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYT26977.1"
                     /translation="MPTAAAVGLIANPAAGRDIRRLTAHASVVPNHEKASIVRRVLHG
                     LAATGVEVVVYLADNAGIIAAAVDGARPPVAMEALGLRAHGWASDSTEAARLLAAAGV
                     GAIVTLGGDGTNRAVAAACGDVPLVAVSTGTNNVFPTMVDGTVAGLAAGLVATGAVAA
                     EQVVRRTKRVEVEAPGVSDFALIDAAACADAFVGSRAIWDVARVRALVLTRAEPWSVG
                     LSAIGGRLRPLAPDEPSGLYVKLGEGPVVTAAVAPGVVASVAVAGWRLLELGEPVTLG
                     GGGTVALDGERELALDLDGSAIAAVTLTGPRVVDIRATLAAAGRA"
     gene            complement(1073..2308)
                     /locus_tag="VEP73_10900"
     CDS             complement(1073..2308)
                     /locus_tag="VEP73_10900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYT26978.1"
                     /translation="MLTEVRMPKLGMAMKKGAVTKWLKAEGETVQEGAELFELATEKI
                     TAVVPAPASGLLGRVVAQLGAELPVGALLALIGEPGEVFPPAHELAASAAEPQPGAPP
                     SPAGAPPAPPGSAAQRGGGGGRGGDGAPVSPAARRRARELGVDVALVPPSAPGKRIMV
                     EDVEAFAATAEAAAEAAAGEVLAPAGRVVPFTGIRKVVAERLSESLRTTAQVTISREV
                     EAGGLVARRAALAAGFEAATGLRLTFTDLLVEAVARLLADHPLLNATLTDQGIVVPDA
                     VHVGIAVALEDGLIVPVIRDAGTRPLAELARDRADLAAKAQAGTLTLDEVEGGTFTIS
                     NLGSFGADAFTPIVNPPQCAILGVGRIVDKPVAVEGRVEVRPLLWLSLTFDHRIVDGA
                     PAARFLRELGDRLATPAPG"
     gene            complement(2329..3348)
                     /locus_tag="VEP73_10905"
     CDS             complement(2329..3348)
                     /locus_tag="VEP73_10905"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYT26979.1"
                     /translation="MAVATRTITFREAVNEALRQEMARDPDVVLMGEDVAGGATVPGF
                     EQEDAWGGVLGVTKGLVQDFGRDRVLDTPISESGFIGAAVGAAATGLRPVAELMFVDF
                     FGVCMDQIFNQGAKLRYMFGGKARVPLVIRTMIGAGFRAAGQHSGCHYSVFTHMPGLK
                     TVVPSTPADAKGLLAAAIRDDDPVLFFEHKLLYDTTGEVPEGEHLVELGRAAVRREGD
                     GATVVAIGRMVNLALEAAEALAAAGVEVEVIDPRTLSPLDDETILASVRKTGRVVVVD
                     EDNPRCSMATDIAALVASRAFDHLDAPPQLVTAPHTPVPFAPVLEDFYVPTPERIAQA
                     VQATL"
     gene            complement(3360..4328)
                     /locus_tag="VEP73_10910"
     CDS             complement(3360..4328)
                     /locus_tag="VEP73_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYT26980.1"
                     /translation="MELTQDKALWIYDRMVTIRAFEERVAKLFADGHIPGFVHLYAGE
                     EAVAVGVCAHLQDTDFITSTHRGHGHCIAKGVPLGAMMAELFGRVDGACKGKGGSMHI
                     ADVSRGMLGANGIVGGGPPLACGAGLTAKLKGTGAVCVCFFGDGAAEQGTTHEAMNLA
                     AIWKLPVVFVAENNGFAESTPVEYHCAASNIADRAASYNIPGISVDGYDAVAMYETAG
                     EAVARARRGDGPSLIEAKTWRYFGHFEGDQVTYRTAEQSAAYREHDPLTWFAKQALER
                     GLLDPAQVEERDQAAEAAVDEAIAFAERSPHPAPEDALTDVYVSYR"
     gene            4521..>4733
                     /locus_tag="VEP73_10915"
     CDS             4521..>4733
                     /locus_tag="VEP73_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HYT26981.1"
                     /translation="MAVLRRPPRTRTLVVAASLLVLVWLLFALAGLYTDLLWFQEVGK
                     AQVFWRILGARFALALVAGAGTGLFLV"
BASE COUNT          634 a         1733 c         1736 g          630 t
ORIGIN      
        1 tcgaggagca ccacctcggc gcctgcccgg cggccggcca gggcggcggc gaccccggcc
       61 ggcccgccgc ccaccaccac caggtcagcc gctgcctcag gcacggccgg ccgccgcgag
      121 ggtggcgcgg atgtcgacca cgcgtgggcc ggtcagggtc actgccgcga tggcgctgcc
      181 gtcgaggtcg agggccagct cccgttcgcc gtccagtgcg accgtgccgc caccgccgag
      241 cgtgaccggc tcgccgagct cgagcagccg ccagcccgcg accgcgacgc tggcgaccac
      301 tccaggcgcg actgcggcgg tcaccaccgg cccctcgccg agcttgacgt acagcccgct
      361 gggttcgtcc ggggccagcg ggcgcaggcg gccgccgatc gctgacaggc cgaccgacca
      421 gggctcggcc cgggtcagca ccagcgcgcg aacccgcgcc acgtcccaga tcgcgcggct
      481 gccgacgaag gcgtcggcgc aggcggcagc atcgatcagg gcgaagtcgg agactccggg
      541 cgcctcgacc tcgacccgct tggtccgtcg caccacctgc tccgccgcca ccgccccggt
      601 cgcaaccaga ccggcggcga gtccagccac ggtgccgtcg accatcgtgg ggaagacgtt
      661 gttggtgccg gtggagaccg ccaccagcgg cacgtcgcca caggcggcgg ccaccgcccg
      721 gttggtcccg tcgccgccga gggtcacgat cgcgccaacc cctgccgcgg ccagcagccg
      781 ggcggcctcg gtcgagtccg acgcccagcc gtgggcgcgc aggccgagcg cctccatggc
      841 gaccggcggg cgggcgccgt cgaccgcggc ggcgatgatc ccagcgttgt cggccaggta
      901 gaccacgacc tccacgccgg tcgcggccaa gccgtgcagc acccggcgga cgatcgaggc
      961 cttctcatgg ttcgggacca ccgaggcgtg ggcggtcagg cggcggatat cccggcccgc
     1021 cgccgggttc gcgatcagcc cgaccgctgc cgctgtgggc acgcggtgcc gcctaccccg
     1081 gcgccggcgt ggcgaggcgg tcgccgagct cccggaggaa gcgggccgcg ggcgcgccgt
     1141 ccacgatccg gtgatcgaag gtgagcgaca gccacagcag cggccggacc tccacccggc
     1201 cctcgaccgc caccggcttg tcgacgatgc ggccgacgcc gaggatcgcg cactgcggcg
     1261 ggttcacgat cggggtgaag gcatcggcgc cgaagctgcc caggttcgag atcgtgaacg
     1321 tgccgccctc gacctcgtcc aaagtcaggg tcccggcctg ggccttggcg gccaggtcgg
     1381 cccggtcccg ggcgagctcg gccagcgggc gggtgcccgc gtcgcggatg accggcacga
     1441 tcaggccgtc ctcaagcgcg acggcaatgc ccacgtgcac ggcgtcgggg acgacgatgc
     1501 cctggtcggt cagggtcgcg ttcagcagcg gatggtcggc gagcaggcgg gccacggcct
     1561 cgaccagcaa gtcggtgaag gtgagacgga gcccggtggc cgcctcgaag ccggcggcga
     1621 gcgcggcccg ccgcgccacc aacccacccg cctccacctc acgcgagatc gtgacctgcg
     1681 cggtggtgcg caggctctcg ctgagccgct cggcgaccac cttgcggatc ccggtgaacg
     1741 gcaccacccg cccggccggg gccagcacct ccccggccgc ggcctcggcg gcggcttcgg
     1801 cggtggccgc gaacgcctcc acgtcctcca ccatgattcg cttgccgggc gcgctcggcg
     1861 gcaccagggc gacgtccacg ccaagctccc gggcgcggcg ccgcgccgcc ggggacaccg
     1921 gtgccccgtc gccgccgcgg cctccaccgc cgccgcgctg ggccgctgac ccgggtggag
     1981 cgggcggcgc tccggctggg gacgggggcg ctcccggctg cggctccgca gcgctggcgg
     2041 ccagctcgtg tgcgggcggg aagacctcgc ccggctcgcc gatgagggcg agcagcgcgc
     2101 cgaccggcag ctcggcgccg agctgggcca ccacccggcc gagcagcccg gaggcgggcg
     2161 ccgggacgac ggcggtgatc ttctcggtcg ccagctcgaa cagctcggcg ccctcctgga
     2221 cggtctcgcc ctcggctttg agccacttgg tgaccgcgcc cttcttcatt gccatgccga
     2281 gtttcggcat gcggacctcg gtaagcatca gcggacctcg cgagagggct agagggtggc
     2341 ctggaccgcc tgggcgatgc gctccggcgt gggaacgtag aagtcctcga gcaccggcgc
     2401 gaacggcacc ggcgtgtgcg gcgcggtgac cagctgcggc ggggcgtcca ggtggtcgaa
     2461 ggcccgggag gcgaccaggg cggcgatgtc ggtggccatc gagcaacgcg ggttgtcctc
     2521 gtcgaccacg accacccggc cggtcttgcg caccgacgcg aggatcgtct cgtcgtcaag
     2581 cggcgacagg gtacgcgggt cgatcacctc cacctcgacg ccagccgcgg ccagcgcctc
     2641 ggccgcctcc aacgcgaggt tgaccatgcg gccgatggcc accacggtgg cgccgtcgcc
     2701 ctcgcgccgc acggcggcac gaccaagctc gacgagatgc tcgccctccg gaacttcgcc
     2761 cgtggtgtcg tagagcagct tgtgctcgaa gaacagcacc gggtcgtcgt cgcggatggc
     2821 agcggcgagc aggcccttgg cgtcggccgg cgtggatggc actaccgtct tgagcccggg
     2881 catatgggtg aagaccgagt agtggcagcc agagtgctgc ccggctgcac ggaagccggc
     2941 cccgatcatg gtgcggatca ccagcgggac gcgggccttg ccgccgaaca tgtagcggag
     3001 cttggcgccc tggttgaaga tctggtccat acagacgccg aagaagtcga cgaacatcag
     3061 ctcggcgacc gggcggagcc ccgtggccgc agcgccgacg gccgcgccga tgaagcccga
     3121 ctcggagatc ggcgtgtcga gcacgcggtc gcgaccgaag tcctgcacca gccccttggt
     3181 gacgccgagg accccgcccc aggcgtcctc ctgctcgaac ccggggacgg tggcgccgcc
     3241 ggcgacgtcc tcgcccatca gcaccacgtc ggggtcgcgg gccatctcct ggcgcagcgc
     3301 ctcgttgacg gcctcgcgga aggtgatcgt gcgggtggcg acggccatgg tgcaccctcc
     3361 taccggtagc tgacgtagac gtcggtgagc gcgtcctcgg gcgccggatg ggggctccgc
     3421 tcggcgaagg caatcgcctc gtcgaccgcc gcctcggcgg cctggtcgcg ctcctcgacc
     3481 tgggcggggt ctagcaggcc gcgctccagc gcctgcttgg cgaaccaggt gagcgggtcg
     3541 tgctcgcggt aggcggccga ctgctcagcg gtccggtagg tgacctggtc gccttcgaag
     3601 tgcccgaagt agcgccaggt cttagcctcg atcaggctgg gcccgtcgcc gcggcgggcg
     3661 cgggccaccg cctcgccggc cgtctcgtac atggcgacgg cgtcgtagcc gtccaccgag
     3721 atgccgggga tgttgtagga ggcggctcgg tcggcgatgt tcgaggccgc gcagtggtac
     3781 tcgactgggg tggactcggc gaagccgttg ttctcggcca cgaacaccac cgggagcttc
     3841 cagatcgcgg ccaggttcat cgcctcatgg gtcgttccct gctccgccgc gccgtcgccg
     3901 aagaagcaaa cgcagaccgc gccggtgccc ttgagctttg cggtgagccc ggcaccgcag
     3961 gccagcggcg ggccgccgcc gacgatcccg ttggcgccca gcatgccgcg ggagacgtcg
     4021 gcgatatgca tggagccgcc cttgcccttg caggcgccgt ccacccgccc gaacagctcc
     4081 gccatcatcg cgccgagcgg gacgcccttg gcgatgcagt ggccgtggcc ccggtgggtc
     4141 gaggtgatga agtccgtgtc ctgcaagtgc gcgcagacac cgaccgcgac cgcctcctcg
     4201 ccggcataga ggtggacgaa gccggggatg tggccgtcgg cgaacagctt tgccacccgc
     4261 tcctcaaagg cccggatggt caccatccgg tcatagatcc agagggcctt gtcctgggtc
     4321 agctccatcg cgtccctccc ggctcctggg ctgcatctgg acaatcctgg cacccaggca
     4381 agagcctacg ccgccgacac agccgcgtca cgaggtcgcg tcgcgaggtc gcatggtcgc
     4441 ggctgggctg ggtggtgtta gggtagagag cgcagtcgtc ccatcggagc cgcccggtgg
     4501 tcgcatcgaa ggggcttggg gtggcggtgc tccgccgccc accgcgcacg cggacccttg
     4561 tggtcgcggc gagcctgctc gtcctcgtct ggctgctgtt cgccctggcc gggctgtaca
     4621 ccgacctgct gtggttccag gaggtcggca aggcccaggt gttttggagg attctcggcg
     4681 cgaggttcgc gctcgcgctg gttgccgggg ctgggaccgg gctgttcctg gtg
//
LOCUS       DASPYY010000694         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1799434, whole genome shotgun sequence.
ACCESSION   DASPYY010000694 DASPYY010000000
VERSION     DASPYY010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799434"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1178)
                     /locus_tag="VEP73_10920"
     CDS             complement(3..1178)
                     /locus_tag="VEP73_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26982.1"
                     /translation="MLHCPRRLAALVLTVLAVAGAMAGPAIAAPRAPDVAARTAVLVD
                     GSTGAVLWQRNEHVPVLVASTTKILTALVAEQVYPPGKVFTVPLAAEQVDGTRFGYRA
                     GMRIRRHELLATLLMVSANDAAETLAVAYPRGGRAGFLLAMQSVAGSLGCTDSTWRSP
                     SGLDVPGHRVSAADLAILARQLLTMPELAALVRARTARYPWPDGHVQVITNHNRFVAA
                     GRDPGAIGVKTGYTVAARQTIVAAERRADRTLIAVVLGAPDIRREEAGVRSMFRYGFV
                     TRPFPGAELIGTAAAQLGRASAQSTTIHDLPGKGGPAPVPVPGGRGLAYRLYAGVLAA
                     PMVLAVAGGAALLMVGLVPVMLFRPRRAVAVPRSSAPGDGRVTAGGARASRYEDRAR"
     gene            1804..>2400
                     /locus_tag="VEP73_10925"
     CDS             1804..>2400
                     /locus_tag="VEP73_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYT26983.1"
                     /translation="MSGRHQRDDPARNPRPYVAITALVLVAALAGTAVAARGSLASLV
                     GGCRGTVRLEIAAGAETAPLLEELAHAYFKDRHKLGNRCVEPSVATTEPAAMVAGFSR
                     GTQAARARGLSPDVWVPPSSAWANLLESLRGGLLPAERPKVATSPLVLAVPRPMAEAL
                     GWPKVPAGWSDLLKALRNPAGWAAFGHRDWGPILFGKTD"
BASE COUNT          328 a          895 c          875 g          302 t
ORIGIN      
        1 gctcagcgcg ctcggtcttc gtaccgggac gcccgcgcgc cgccggccgt gacccggccg
       61 tccccgggtg cggagctcct cggcacggcc accgcacgcc tcggccggaa gagcatgacc
      121 ggcacgagcc cgaccatgag cagggccgcc ccgcccgcca ccgcgagcac catgggcgcg
      181 gccagcacgc cggcgtagag ccggtaggcg aggccgcgac cgccggggac ggggaccggc
      241 gccggtccgc ccttgcctgg caggtcgtgg atggtcgtgg actgcgccga cgcccggccg
      301 agctgcgccg cggccgtgcc gatgagctcc gcacccggga acggccgggt cacgaacccg
      361 tagcggaaca tcgaccggac gccggcctcc tcgcgccgga tgtccggcgc gccgagcacc
      421 accgcgatca gggtacggtc ggcgcgccgc tcggcggcga cgatcgtctg cctggccgcc
      481 accgtgtacc cggtcttcac gccgatggcg cctgggtcgc ggccggcggc cacgaagcgg
      541 ttgtggttcg tgatcacctg cacgtgcccg tcgggccagg ggtacctggc ggtccgggcc
      601 cggaccagcg cggcgagctc cggcatggtg agcagctgcc tggcgaggat cgcgaggtcg
      661 gcggcgctca cccggtggcc ggggacgtcg agcccggacg ggctgcgcca ggtcgagtcg
      721 gtgcagccga gcgacccggc cacggactgc atagccagca ggaacccggc ccggccgccg
      781 cgcgggtagg cgaccgcgag cgtctcggcc gcgtcgttgg ccgagaccat cagcagcgtc
      841 gccagcagct cgtgacggcg gatccgcatc ccggcccggt agccgaaccg ggtgccgtcc
      901 acctgctcgg cggcgagggg gacggtgaac accttcccgg gcgggtagac ctgctcggcc
      961 accagcgcgg tgaggatctt ggtggtcgat gccacgagca ccgggacgtg ctcgttccgc
     1021 tgccacagga cggcgcccgt gctcccgtcg acgagcacgg cggtccgggc ggcgacgtcg
     1081 ggcgcccggg gagcggcgat ggcgggcccc gccatcgccc cggccaccgc gaggacggtg
     1141 agcacgagcg cggcgagccg gcgcggacag tggagcacac cggtagcctc accgacttgc
     1201 cgtcgtggtg caacgccctc gaaccagacg aaccagatcg caaccgtcgg aagcaggcgc
     1261 tggttcggtg cgctcatccg ccctccgctc tgcggccggg ggcccgccaa tcctgccccg
     1321 cgacgacgcc aggcgccgcg cggggaggtc gcctgggacc ccgcgccggc ccttcgacca
     1381 gcgcagcggt cccgttcagc gagagctgcc tccggccctt cgatcggagg gcgggctccc
     1441 gctggcgata tcgtcagggt gccatgccgg cttgaccttc gcccagccgt tgcgctcccg
     1501 attctgccta cgcaaacacc tacgcaggca cggcggcctg ctccggacgc cgaattcggg
     1561 tacggattgg caatccgaat gagcagctcg cagaagacga acctgtcatc caactcgatg
     1621 cccgtgatct tatctaggca atacggtcga accgtcggta tttttgccaa ttcccgccca
     1681 ccgccctatc gttgcccgcg ccgaacgcgc tccgggtgcg ccctcttttc cgcgcccgcc
     1741 gccgatggga cgggcaagga cagacggcga ggaggcaggc ggcggtagag gtgaaaggaa
     1801 gacgtgagcg gacgccatca gcgtgatgac cctgcgcgca acccccgccc gtacgtcgcc
     1861 atcacggcgc tcgtcctggt agccgccctc gcgggcacgg cagtggccgc ccgcgggagc
     1921 ctcgcctcgc tcgtcggagg gtgccgaggc acggtcaggc tggagatcgc cgccggggcc
     1981 gagaccgctc cgctgctcga ggagctggcc cacgcctatt tcaaggaccg tcacaagctg
     2041 ggcaaccgct gcgtggagcc gagcgtcgcg acgacggagc ccgcagcgat ggtggccggc
     2101 ttctcccggg gcacgcaggc cgcgcgggcc agggggctgt cgcccgacgt ctgggtgcct
     2161 ccctcgtcgg cctgggccaa cctcctcgag agcctgcgcg gcgggctgct cccggccgag
     2221 cggcccaagg tggccacgtc gccgctggtg ctcgccgtgc cccgcccgat ggccgaggcg
     2281 ctcggctggc cgaaggtccc cgccggctgg agcgacctgc tgaaggccct ccgcaacccg
     2341 gccggctggg ccgcgttcgg gcaccgcgac tgggggccaa ttctgttcgg caagaccgac
//
LOCUS       DASPYY010000695         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2641657, whole genome shotgun sequence.
ACCESSION   DASPYY010000695 DASPYY010000000
VERSION     DASPYY010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641657"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VEP73_10930"
     CDS             <1..829
                     /locus_tag="VEP73_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HYT26984.1"
                     /translation="GGAAGAAALANRRLRQRPVARALLLAGLTWTALGGRSLARTGRQ
                     LGEAVRAGDLERARQLAPALVGRDPRGLDGAELCRAAVESLAENTADAVVGPLLWGAL
                     AGPAGVAAYRAANTLDAMVGHRNPRYLRFGWAAARLDDLLNWPVARLGAALTCLLAAT
                     GATTRAERWRAWRVLRRDGAAHPSPNAGRMEAAFAGALGVRLGGRNRYGDQVEERPTL
                     GDGPAPGPDDVDRAVRLSLRVGAATVAVSVALSALTTMFWQRRPRSATRGAHRAGAR"
     gene            826..2361
                     /locus_tag="VEP73_10935"
     CDS             826..2361
                     /locus_tag="VEP73_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HYT26985.1"
                     /translation="MTAVRGRSGVLLVAGTASDAGKSVVVAGICRHLARQGLKVAPFK
                     AQNMALNSAVTADGAEIGRAQVAQAAAAGAAPEAAMNPILLKPAGERVSQVVVMGQPF
                     ATVDARSYQDLKPQLRGVVLEALADLRRRYDVVICEGAGSPAEINLRANDLVNLGLAR
                     AAGLPVVVVGDIDRGGVFASLFGTLALLDADDQALVAGFLINKFRGDHTVLAPGLEQL
                     RALTGRPVLGVLPWRRGLWLDVEDSLALEAPRDGMLPPLGADSLVVAVARLRWLSNFT
                     DFDALAAEPGVVVRFTQSPAELLSADLAVLPGSKATAEDLAWLRARGLDKAITTRARR
                     GLPTLGICGGYQMLGGRITDRDGVESSASDLAGLGMLPVETEFAASKTLTNPTGTAPA
                     FGGVPAAGYEIHHGRSRRLGGEPVLASSSGDEGCRQGAVVGLAWHGIFEGDAFRRSFL
                     TWVAAERGLDWMPGERPFAAVRAERLDALGDLIAHHTDTAALDHLIHHGAPDGLPFIG
                     AVR"
BASE COUNT          293 a          961 c          898 g          319 t
ORIGIN      
        1 cggcggtgcc gccggcgctg cggcgctggc caacaggcgc ctgcggcagc ggccggtggc
       61 gcgggcgctg ctgcttgccg ggctcacctg gacggcactc ggcggccgtt ccctggcgcg
      121 gacgggccgc cagctcggcg aggcggtgcg tgccggcgat ctggagcgcg cccgccagct
      181 cgcccccgcg ctggtggggc gcgacccgcg cgggctcgac ggcgccgagt tgtgccgcgc
      241 cgccgtcgag tcgctggccg agaacaccgc cgatgcggtg gtcgggccgc tgctgtgggg
      301 cgccctcgcc ggtccggccg gcgtcgccgc ctaccgggcc gccaacaccc tggacgctat
      361 ggttggccac cggaatcccc ggtacctccg cttcggctgg gcggccgcgc gcctggacga
      421 cctgctcaac tggccggtcg cgcggctcgg cgcggcgctc acctgcctcc tcgccgctac
      481 cggcgctacc acccgcgccg agcgctggcg ggcctggcgg gtgctgcgac gcgacggcgc
      541 ggcgcatccg agccccaacg ccggccgcat ggaggccgcc ttcgcgggcg cgctcggtgt
      601 ccgcctcggt ggccgcaacc gctacggcga ccaagtcgag gagcggccca ccctcggcga
      661 cggcccagcg cccggccccg acgacgtgga tcgcgcggtg cgcctgtccc tccgggtggg
      721 cgcggcgacg gtcgcggtct ccgtggcgct gtccgccctc acgacgatgt tctggcagcg
      781 gaggccccgg tcggcaacac ggggagcgca ccgggcaggt gcacgatgac ggcggtgcgt
      841 gggcgaagcg gcgtgctgct ggtcgctggg accgcctcgg acgcgggcaa gagcgtcgtg
      901 gtcgccggga tctgccgcca tctggcacgg cagggcctca aggtggcgcc gttcaaggcg
      961 cagaacatgg cgctgaactc cgccgtcacc gctgacggcg ccgagatcgg ccgcgcgcag
     1021 gtagcacagg ccgccgccgc cggtgccgcg cccgaggcgg cgatgaaccc gatcctgctc
     1081 aagcccgccg gcgagcgcgt cagccaggtg gtggtgatgg ggcagccgtt cgccaccgtg
     1141 gacgcccgct cctaccagga cctcaaaccg caactccggg gcgtggtgct ggaggcgctg
     1201 gcggacctgc ggcgacgcta cgacgtggtg atctgcgagg gcgccggcag ccccgctgag
     1261 atcaacctcc gagcgaatga cctggtcaac ctgggcctgg cgcgggcggc cggcctgccg
     1321 gtggtggtgg tcggcgacat cgaccgcggc ggggtgttcg ccagcctgtt cggcaccctc
     1381 gccctgctcg acgcggacga ccaggcgctg gtcgccgggt tcctgatcaa caagttccgc
     1441 ggcgaccaca cggtgctcgc acccggcctg gagcagctcc gcgccctcac cggccggccg
     1501 gtgctgggcg tgctgccgtg gcggcgcggg ctgtggctgg acgtggagga ctccctcgcg
     1561 ctggaggcgc cccgcgacgg catgctgcca ccactcggcg cggactcgct ggtggtggcg
     1621 gtggcaaggc tgcgctggct gtcgaacttc accgacttcg acgccctcgc cgccgagccg
     1681 ggggtggtgg tgcgcttcac ccagtcgccg gccgagctgc tcagcgccga tctggccgtg
     1741 cttcccggca gcaaggcgac cgccgaggac ctcgcctggc tgcgcgcccg cggcctcgac
     1801 aaggccatca ccacccgggc gcggcgcggc ctgccgaccc tcgggatctg cggcggctac
     1861 cagatgctcg gcgggcgcat caccgaccgg gacggcgtcg agagcagcgc cagcgacctt
     1921 gccggcctcg gcatgctccc agtcgagacc gagtttgccg ccagcaagac gctcaccaac
     1981 ccgaccggca ccgcccccgc gttcggcggc gtcccggcag ccgggtacga gatccaccac
     2041 ggccgcagcc gccgcctcgg cggcgagccg gtcctcgcca gcagcagcgg cgacgagggc
     2101 tgccgccaag gcgcggtggt cggacttgcc tggcacggca tcttcgaggg cgacgccttc
     2161 cgccgcagct tcctcacctg ggtcgcggcc gagcgcggcc tcgactggat gcccggcgag
     2221 cgccccttcg ccgccgtccg cgccgaacgc ctcgacgccc tcggcgacct gatcgcccac
     2281 cacaccgaca ccgccgccct cgaccatctc atccaccacg gcgctccaga cggcctcccg
     2341 ttcatcggag cggtccggtg atcctgttcc tcaccaccgc cgataccgag atcctggcga
     2401 cacaccgcgc cctttccaca ttgcctggcg ggtttccgac ggtgcgcgcc gccaacccga
     2461 ccgccaactc c
//
LOCUS       DASPYY010000696         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1941297, whole genome shotgun sequence.
ACCESSION   DASPYY010000696 DASPYY010000000
VERSION     DASPYY010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941297"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..829)
                     /locus_tag="VEP73_10940"
     CDS             complement(35..829)
                     /locus_tag="VEP73_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HYT26986.1"
                     /translation="MQEEELTYGRYLRLDELLALQRPRSDPPHPEELHFIIVHQALEL
                     WMKLALHDLERVIGFLDDDAWPRALALLARVNGNLATGLEQMRSLQTLPPADLQRFRS
                     YLGTASGSQSVQFRELELLSGLREPAYLKALEIEHRGRLPEVLARRLAQRSLAEAHAG
                     AAGRLGIADWAAFYTDPGERTDFYLVCEALVDYDTRWARWRQEHVALVERTLGTHARG
                     TGGTAITYLQRTVGYRFFPVLWALRDELVVRGGGELVGRPPRPVAG"
     gene            1031..1822
                     /locus_tag="VEP73_10945"
     CDS             1031..1822
                     /locus_tag="VEP73_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT26987.1"
                     /translation="MRRTLVLLGGVGLLALLLLPATGSASPGSAAAAPAAARVRAQTA
                     QGTLDAGRLLYLQSCASCHGVDPAGPSNYPTVPSLKGVGGAAAVDWVLRTGRMPWRST
                     VGPAITRGKPKFNQSDARAIALYVGTAVGDTDIPTVDPGQGDEIRGRGLYAAACAACH
                     GMNGAGAALGGSNIAPSLQDVAPLDVAEAMKIGPGVMPVFQGGVYNGDGVNSIAAYVQ
                     GLKTGKDDVGGAPIGGKGPVPEGFVAWVVGLGALILASRWIGGKS"
     gene            1893..2795
                     /locus_tag="VEP73_10950"
     CDS             1893..2795
                     /locus_tag="VEP73_10950"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT26988.1"
                     /translation="MEDGQRRDRPGDELPSDQRRAERRIAIAFGITAACGVALFFVYF
                     SGGQTQLEGLLFGLAFTSLGWGVAAWAAKLLDAREVSEDRHPLTSGPAGRKAFEQALL
                     EEAGPLLRHPRRSFLVKMLVGAGGALGLALLLPALSLGPAPGRSLEETGWRRGKRVVD
                     EQGKPVAFDELPADGYLTVFPEGEAGRADSQALLIKVPAGTLQLSAAQLAGVPGGTHV
                     AYSKICTHAGCPVGLYRAETRSLLCPCHQSQFDVLRGCKPYFGPAARPLPQLPLTVDS
                     GGTLVALGDFDAPVGPAFWHRSKS"
     gene            2792..>3769
                     /locus_tag="VEP73_10955"
     CDS             2792..>3769
                     /locus_tag="VEP73_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HYT26989.1"
                     /translation="MTMLETTIKYVDERLGAASLARKTLRKVFPDHWTFLLGEIALFS
                     LVVLVFTGLFLTLFYTPDARLLTYQGAYTPLHGQQVSAAYNSVLRLSFEVRAGLIMRQ
                     THHWAALVFVAAIVVHLCRIFFTGAFRRPRELNWLIGFVLLLLAMGEGFTGYSLPDDL
                     LSGIGLRIAYSVVLAIPLIGTWVAFLLFGGEFPAPDIISRLFVFHVMLLPALLFALVG
                     LHLAILVLQKHTQFPGPGRTERNVVGKRLWPEQTFKSVSLLLFTTAILAALGGLAQIN
                     PIWQYGPYEPFNVTSPAQPDWYIGWLDGALRLAGPWSFKIFGYTISEVIW"
BASE COUNT          467 a         1408 c         1337 g          557 t
ORIGIN      
        1 cgccgcgccc ctctcgtcga cggttccggt cgccctagcc tgccacggga cgcggcggtc
       61 tgccgacgag ctcgccgccg ccccggacga cgagctcgtc gcgcagcgcc cacagcaccg
      121 ggaagaaccg gtagcccacc gtgcgctgca ggtaggtgat ggccgtgccg cccgtgcctc
      181 tggcgtgggt gccgagcgtc cgctcgacca gcgcgacgtg ctcctggcgc cagcgcgccc
      241 agcgggtgtc gtagtccacc agcgcctcgc agacgaggta gaagtcggtc cgctcgcccg
      301 ggtcggtgta gaacgcggcc cagtcggcga tccccagccg cccggccgcg ccggcgtggg
      361 cctcggccag ggagcgctgc gccaggcggc gggccaggac ctcgggcagc cgcccgcggt
      421 gctcgatctc gagcgccttc aggtaggccg gctcgcgcag gccgctgagc agctcgagct
      481 cgcggaactg caccgactgg gagccgctgg cggtgccgag atagctgcgg aaccgctgca
      541 ggtcggcggg cgggagcgtc tgcaggctgc gcatctgctc gagcccggtc gcgaggttcc
      601 cgttgacccg cgcgagcagc gcgagcgcgc gcggccacgc gtcgtcgtcg aggaacccga
      661 tgacccgctc gaggtcgtgg agggcgagct tcatccacag ctcgagcgcc tggtggacga
      721 tgatgaagtg cagctcctcg gggtggggag ggtccgagcg tggccgctgc agcgccagca
      781 gctcgtcgag ccgcaggtac cggccgtagg tcagctcctc ctcctgcatc gtccccacca
      841 gtccttcccg ttctgtttcc cgttcggcac gcctgtccgt gtcccggacg gcctcgggcg
      901 agcgtgcttg gacgcgcccc cggacgggta ccgactgccc gccggagcag gtagtgattg
      961 ccgaccggat gtgccagccc tagcatgcac ccaatcggag gcacgcctcg gcgcaggaga
     1021 aggagcgacc gtgcgaagga ccctcgtgct gctcggcggt gtcggcctcc tcgccctgct
     1081 gctcctgccg gccaccggca gcgcgtcgcc gggctcggcc gcggcggcgc ccgcggccgc
     1141 ccgggtgcgc gcccagaccg cccagggcac cctcgacgcc ggccgcctcc tctacctgca
     1201 gagctgcgcg tcgtgccatg gcgtcgaccc ggccggcccg agcaactacc ccacggtgcc
     1261 gtccctgaag ggcgtcggcg gcgcggccgc cgtcgactgg gtgctgcgca ccggccgcat
     1321 gccgtggcgg tcgaccgtgg gcccggcgat cacgcgcggc aagcccaagt tcaaccagtc
     1381 cgacgcccgc gccatcgcgc tctacgtcgg caccgccgtg ggcgacaccg acatccccac
     1441 cgtcgacccc ggccaggggg acgagatccg gggccgcggg ctgtacgcgg cggcgtgcgc
     1501 cgcctgccac ggcatgaacg gcgcgggggc cgccctcggc gggtcgaaca tcgccccgag
     1561 cctgcaggac gtggcccccc tggacgtcgc cgaggcgatg aagatcggcc ccggggtcat
     1621 gccggtgttc cagggcggcg tctacaacgg cgacggcgtg aactcgatcg ccgcctacgt
     1681 gcagggcctc aagacgggca aggacgacgt gggcggcgcg ccgatcggcg gcaagggccc
     1741 cgtcccggag gggttcgtgg cctgggtggt cggcctcggc gcgctcatcc tggcgtccag
     1801 gtggatcggt gggaagagct gaggggaagc gctgagaccg ggcgcgggcg gccgcggggc
     1861 aactggtgga gcacgaggac gggagtcggg acgtggagga cggacagcgc agggaccggc
     1921 ccggcgacga gctgccgagc gaccagcgcc gcgccgagcg caggatcgcg atcgcgttcg
     1981 gcatcaccgc ggcctgcggc gtcgcgctgt tcttcgtgta cttctcgggc gggcagaccc
     2041 agctcgaggg gctcctgttc gggctcgcct tcacgagcct cggctggggg gtcgcggcct
     2101 gggccgcaaa gctgctcgac gcgcgggagg tgagcgagga ccgccacccg ctcacctccg
     2161 gcccggccgg caggaaggcg ttcgagcagg cgctcctcga ggaggccggc cccctgctcc
     2221 gccacccgcg ccgctcgttc ctggtcaaga tgctcgtcgg cgccggtggc gcgctcggcc
     2281 tcgcgctgct gctgccggcc ttgtcgctgg ggcccgcgcc cggccgctcg ctcgaggaga
     2341 ccggctggcg caggggcaag cgggtggtcg acgagcaggg caagccggtg gcgttcgacg
     2401 agctgcccgc ggacggctac ctgaccgtgt tcccggaggg cgaggccggc cgggccgact
     2461 cccaggccct gctcatcaag gtgccggccg gcacgctcca gctctccgcc gcgcagctcg
     2521 cgggcgtccc gggcggcacc cacgtcgcct actcgaagat ctgcacccac gcgggctgcc
     2581 cggtcggcct gtaccgggcg gagacccgct cgctcctctg cccctgccac cagtcgcagt
     2641 tcgacgtgct gcgaggctgc aagccgtact tcggcccggc tgcccggccc ctgccccagc
     2701 tgcccctgac ggtcgacagc ggaggtaccc tggtggcgct gggcgacttc gacgcacccg
     2761 tcgggcccgc gttctggcac cggagcaagt catgacgatg ctcgagacca cgatcaagta
     2821 cgtcgacgag cggctcgggg cggccagtct cgcgcgcaag acgctgcgca aggtgttccc
     2881 cgaccactgg acgttcctgc tcggcgagat cgccctgttc agcctcgtgg tgctggtctt
     2941 caccgggctg ttcctgaccc tgttctacac ccccgacgcc cgtctcctga cctaccaggg
     3001 cgcctacacg ccgctgcacg gccagcaggt ctcggccgcc tacaactccg tgctgcggct
     3061 gtcgttcgag gtccgggccg ggctcatcat gcgccagacc caccactggg ccgcgctggt
     3121 gttcgtcgcc gcgatcgtgg tccacctgtg ccgcatcttc ttcaccggcg cgttccgccg
     3181 cccccgcgag ttgaactggc tgatcgggtt cgtcctgctg ctgctcgcga tgggcgaggg
     3241 cttcaccggc tactcgctgc ccgacgacct gctctccggc atcgggctgc ggatcgccta
     3301 ctcggtggtg ctcgccatcc ccctgatcgg cacctgggtc gcgttcctgc tgttcggcgg
     3361 ggagttcccc gcccccgaca tcatctcgcg gctgttcgtc ttccacgtca tgctgctgcc
     3421 cgcgctgctg ttcgccctcg tgggcctgca cctcgcgatc ctcgtgctgc agaagcacac
     3481 ccagttcccc ggcccggggc ggaccgagcg caacgtcgtc ggcaagcgcc tgtggcccga
     3541 gcagacgttc aagtcggtgt cgctgctgct gttcaccacc gcgatcctcg ccgccctcgg
     3601 cggtctcgcc cagatcaacc cgatctggca gtatggcccc tacgagccgt tcaacgtcac
     3661 gtcgccggcc cagcccgact ggtacatcgg ctggctcgac ggcgcgctgc gcctcgccgg
     3721 gccgtggagc ttcaagatct tcggctacac gatctcggag gtgatctgg
//
LOCUS       DASPYY010000697         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_392794, whole genome shotgun sequence.
ACCESSION   DASPYY010000697 DASPYY010000000
VERSION     DASPYY010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392794"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2656
                     /locus_tag="VEP73_10960"
     CDS             <1..>2656
                     /locus_tag="VEP73_10960"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HYT26990.1"
                     /translation="PRLPDGHDPRRLLLGDVDGDGVADLVYLDRGRVLLWGNQTGNAW
                     TPQPVTVTGTPDVVDSDAVQLADLYGTGMAGVLWSRAADGSGASPLRFLDLTGGVKPY
                     LLDAMDNHLGALTTVGYRPSTSYFLADRADPATRWRTTLPFPVHVVARVEVTDQLSSG
                     RLVTEYRYHHGYWDGVEREFRGFAMVEQLDSETFHGLAAHYSPPTLTRSWFHVGPVAA
                     AEAGDWAELDLGHEYWPGDAPQLRRPPEMVDALARLPRPARRDALRALRGQVLRTELY
                     ALDGSDRQDRPYTVTESLPGVRAESERVWFPFSLAQRTTQWERGSDPMTQFAFTGGHD
                     QYGFATRQLTVAVPRGRDPRAADPAASRPYLATYATTEYARRDDPERYIVDRVARTTS
                     YEVQNDGRSSVPALRDAVLVGPSLSDPVALRVIGHSRTFYDGQAYEGLPLGELDEHGL
                     PTRSESLAFTDSFLDELFDPADPLAVGPRPAYLDPAGTTWPEEYPQEFRDLLPQRAGY
                     LHYRDGELPGSPGGYYVAGARHRYDVHDPARTPRGLLLGSLDPLAAESRIAYDEHDLL
                     PTRTTDAAGLTTEARYDPRVLHPRDVTDANGNGRAVTYSPIGLVAAQFVRGKDGQGDA
                     TAPSVRMDHDLLAFAERGQPVSVRSARRVHHDTEPDVPAAERDEVIVSVQYSDGFGRL
                     LQTRAQAEDTLLGDSAFGGGVIPAEQGAPVPATAGRTRQPGEPDNVIVSGWQTYDNKG
                     RVVEKYEPFFATGYDYAPPLDAQLGQKVTVFYDPRGHAVRTVNPDGSEQLVVLGVPVD
                     LAHPDVFAPTAWESFTYDANDNAGRTHGAAAEAYRGHRDTPASIEVDALGRTVVATAR
                     NGPDPDADWYVTRSTYDVEGNLVAVTDAL"
BASE COUNT          365 a         1070 c          910 g          311 t
ORIGIN      
        1 cgccccggct gcccgacggc cacgatcccc gccggctgct gctgggcgac gtggacggcg
       61 acggggtggc cgacctggtc tacctcgatc gcgggcgggt gctgctgtgg ggcaaccaga
      121 ccggcaacgc ctggacaccc cagccggtga cggtcaccgg caccccggac gtggtggaca
      181 gcgacgccgt gcagctcgcc gacctgtacg gcaccgggat ggccggggtg ctgtggagcc
      241 gggccgccga cgggtcgggc gcgtcgccgc tgcggttcct cgatctcacc ggcggggtca
      301 agccgtacct gctcgacgcg atggacaacc acctcggcgc gctgacgacg gtgggctacc
      361 gcccgtcgac gtcgtacttc ctcgccgacc gggccgaccc ggcgacccgc tggcgcacca
      421 cgctgccctt cccggtgcac gtggtggccc gggtcgaggt gaccgaccag ctctcctccg
      481 ggcgcctggt caccgagtac cgctaccacc acggctactg ggacggcgtc gagcgtgagt
      541 tccgcggctt cgcgatggtc gagcagctcg actccgagac cttccacggg ttggccgcgc
      601 actactcgcc gccgacgctc accaggagct ggttccacgt cgggccggtc gccgccgcgg
      661 aggcgggcga ctgggccgag ctcgacctgg gtcacgagta ctggccgggc gacgccccgc
      721 agctccgccg gccgcccgag atggtcgacg ccctcgcccg gctgccgcgc ccggcccgcc
      781 gggacgcgct gcgggccctg cgcgggcagg tgctgcgcac cgagctgtac gcgctggacg
      841 gcagcgaccg ccaggaccgg ccgtacacgg tgaccgagtc gctccccggg gtccgggcgg
      901 agtcggagcg cgtctggttc ccgttctcgc tcgcccagcg gaccacccag tgggagcgcg
      961 gcagcgaccc gatgacccag ttcgccttca ccggcgggca cgaccagtac ggcttcgcca
     1021 cccggcagct cacggtcgcg gtgccgcgcg gccgcgaccc gcgggcggcc gacccggcgg
     1081 cgtcgcggcc gtacctggcc acctacgcca ccaccgagta cgcccgccgc gacgaccccg
     1141 agcgctacat cgtggaccgg gtcgcgcgca ccaccagcta cgaggtgcaa aacgacgggc
     1201 ggtcgagcgt gcccgccctc cgcgacgccg tcctcgtcgg cccgtcgctg agcgacccgg
     1261 tggcgctgcg cgtcatcggc cacagccgga ccttctacga cgggcaggcg tacgagggac
     1321 tgccgctggg cgagctcgac gagcacgggc tgcccacccg gagcgagtca ctggcgttca
     1381 ccgacagctt cctggacgag ctgttcgacc cggccgaccc gctcgccgtc ggcccgcgcc
     1441 cggcctacct cgacccggcc ggcacgacct ggcccgagga gtatccgcag gagttccgcg
     1501 acctgctccc ccagcgggcc gggtacctgc actaccggga cggcgagctg cccggctcgc
     1561 ccggcggcta ctacgtcgcc ggcgcccgcc accgctacga cgtccacgac ccggcacgga
     1621 cgccccgcgg gctgctgctc ggctcactcg acccgctcgc cgcggagagc cgcatcgcct
     1681 acgacgagca cgacctgctg ccgacgcgca cgaccgacgc cgccggactc accaccgagg
     1741 cccgctacga cccgcgcgtg ctccaccccc gcgacgtcac cgacgcgaac ggcaacggcc
     1801 gcgccgtcac ctactccccc atcgggctgg tcgcggccca gttcgtccgc ggcaaggacg
     1861 ggcagggcga cgccaccgcc ccgagcgtcc ggatggacca cgacctgctc gcgttcgccg
     1921 aacgcggcca gccggtctcg gttcggtcgg cccgccgggt gcaccacgac accgagccgg
     1981 acgtcccagc cgccgagcgc gacgaggtca tcgtgtcggt ccagtactcc gacgggttcg
     2041 gccggctgct gcaaacccgg gcgcaggccg aggacacgct gctcggcgac tcggcgttcg
     2101 gcggcggagt gattcccgcc gagcaaggcg ccccggtgcc cgcgaccgcc ggccggaccc
     2161 ggcaaccggg cgagcctgac aacgtgatcg tcagcggctg gcagacctac gacaacaagg
     2221 gccgcgtcgt cgagaagtac gagccgttct tcgccaccgg ctacgactac gccccgccgc
     2281 tcgacgccca gctcggccag aaggtcaccg tcttctacga ccctcgcggc cacgcggtcc
     2341 gcaccgtcaa cccggacggc agcgagcagc tggtcgtgct cggcgtgccg gtcgacctcg
     2401 cccacccgga cgtgttcgcc ccgaccgcat gggagtcgtt cacctacgac gccaacgaca
     2461 acgccggccg gacccacggc gccgccgccg aggcctaccg cggccaccgg gacaccccgg
     2521 cgagcatcga ggtcgacgcg ctcgggcgca cggtcgtggc caccgcccgc aacgggccgg
     2581 accccgacgc cgactggtac gtcacccgct ccacctacga cgtcgagggc aacctggtcg
     2641 cggtcaccga cgcgct
//
LOCUS       DASPYY010000698         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2221002, whole genome shotgun sequence.
ACCESSION   DASPYY010000698 DASPYY010000000
VERSION     DASPYY010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221002"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEP73_10965"
     CDS             complement(<1..587)
                     /locus_tag="VEP73_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYT26991.1"
                     /translation="MPMGRGDGRPRARVRPDLILPRHAGDAVRLVAAVAVLLLSAELV
                     HRNRVARLEVDAFRIVNDLPGTLYPFVWPVMQLGNILAVPVVAAAAGLTRRFRLAANL
                     AMAGAGCWLLAKLVKRLVERPRPSFFLHDVHIRGTADTGLGYVSGHAAVSVALASVAS
                     PYLPRRARRVVWALAAVVCLSRMYVGAHLPLDVVGG"
     gene            1053..1277
                     /locus_tag="VEP73_10970"
     CDS             1053..1277
                     /locus_tag="VEP73_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26992.1"
                     /translation="MTPDSSTSRRRGVGGLLLACLLAFGLIAGWLHFFAPRSAPPGVT
                     LTDVHGVADLQARFNADRGMPRLVVIFSPT"
     gene            1305..1643
                     /locus_tag="VEP73_10975"
     CDS             1305..1643
                     /locus_tag="VEP73_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT26993.1"
                     /translation="MQSRILAANPHARLRVYALWTDKLFGDARNRWDAAGLTDPRVTH
                     LWDGPDLAGDWLAGNTPGYQGSDWDTYLLFGPEATWTTRPTPLRGWGGPVDEHVDDLD
                     RALRPLLATR"
     gene            complement(1687..>2689)
                     /gene="mtnA"
                     /locus_tag="VEP73_10980"
     CDS             complement(1687..>2689)
                     /gene="mtnA"
                     /locus_tag="VEP73_10980"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HYT26994.1"
                     /translation="EVVDQTALPERLSVLRLASVAEVVDALRRLVVRGAPAIGVCAAF
                     GVVVGLDERGGRDVAEARAGLGRVAAELEAARPTAVNLRWAVRRVAAAAGAGTDPAAM
                     RRLALEEATRILEEDRAACRRIGEHGRAELASRHRVLTHCNTGRLATAGLGTALGVVY
                     AKAAARHPVQVWATETRPLLQGSRLTAWELVDAGIPVTLLPDAATGAAMAQGLVDSVL
                     VGCDRVARNGDTANKIGTYNLAVLARASGIPFYVAGPLSSFDPDAEDGSAIVVEERAG
                     AEVRFLGGARLAPDGAEVWNPAFDVTPADLVTAFVTDAGVLRPPFADSIAGALRGRP"
BASE COUNT          339 a         1044 c          991 g          315 t
ORIGIN      
        1 ccgccgacga cgtcgagcgg caggtgcgcg ccgacgtaca tgcgggacag gcagacgacg
       61 gcggccagcg cccagaccac cctccgcgcc cgcctgggga ggtacgggct cgccaccgag
      121 gcgagcgcga ccgacacggc ggcgtggccg gagacgtagc cgagccccgt gtcggccgta
      181 ccgcggatgt ggacgtcgtg gaggaagaag ctgggccgcg gccgctcgac cagtcgcttg
      241 accagcttgg cgagcagcca gcagcccgcg ccggccatgg cgaggttcgc ggccaggcgg
      301 aaccgccggg tgaggccggc ggcggccgcc accaccggca cggcgaggat gttgccgagc
      361 tgcatgaccg gccacacgaa cgggtagagc gtgcctggca ggtcgttcac gatccggaag
      421 gcgtcgacct cgagccgcgc cacgcggttg cggtgcacca gctccgcgct caggagcagg
      481 accgcgaccg ccgcgacgag gcggaccgcg tcgccggcgt gccgcggcag gatcaggtcc
      541 gggcggaccc gggcccgtgg ccgcccatcc ccccgcccca tcggcatctc cgctcctcga
      601 cctgtcctcg acctgcgccg gcggcgccca tcctggcgac ctcacgctcc cgcgatggta
      661 ccgtgcgccg cccaccatcg cggtcgcggg catcgtgctc gcgctggcct tcctgcccag
      721 gcgcgccacc gcgaccgcgg caacgggggc tgggccggct gcacgggagg atgaggctgt
      781 tgtccaacca tgatcgaccg agcgatggcc gaccggcggt cggaggtctc gcccagcacg
      841 ttccttcgct acttccccac caaggcagac gtcgtgctgt acgacgtcct cgacccgccc
      901 cttctcgagg cgttgcgcgc acagccgacc gagctgagcc cgatccaggc cctgcgcgga
      961 gcgatgcgcg cggtgttacg ctgcgctgat ggacgccggc ttggcgcacc tggaggccgg
     1021 cctgccgctg tgagagtccg gaggggagac acttgacgcc cgactcctcc acctcccgcc
     1081 gccgtggcgt gggtggcctc ctgctcgcct gcctgctggc gttcggcctg atcgccggct
     1141 ggctgcactt cttcgcgccc cgctcggcgc cgcccggggt gaccctgacc gacgtgcacg
     1201 gcgtcgccga cctgcaggcg cggttcaacg ccgaccgtgg catgccgcgg ctggtcgtga
     1261 tcttctcccc cacctgaacg gagtgccgcc acggcgccgg tctggtgcag tccaggatcc
     1321 tcgcggcgaa cccgcacgcc cgcctgcgcg tgtacgcgct gtggaccgac aagctgttcg
     1381 gggacgctcg caaccgctgg gacgccgccg ggctgaccga cccacgcgtg acgcacctgt
     1441 gggacggccc cgacctggcg ggcgactggc tggccggcaa cacccccggc taccagggca
     1501 gcgactggga cacctacctg ctgttcggcc cggaggcgac ctggaccacc cggcccaccc
     1561 cgctgcgcgg ctggggcggg cccgtcgacg agcacgtcga cgacctcgac cgggcgctgc
     1621 gtcccctgct cgccacgagg tgaccgcggg cgccgctggc ggcgcccggc gcccggcgcc
     1681 cgccggtcag ggccgcccac gcagcgcgcc cgcgatcgag tcggcgaacg gggggcggag
     1741 cacgccggcg tcggtgacga acgcggtgac gaggtcggcc ggggtcacgt cgaacgcggg
     1801 gttccagacc tcggcgccgt ccggggcgag gcgcgcgccg ccgaggaagc ggacctccgc
     1861 gccggcgcgc tcctccacga cgatggcgct gccgtcctcc gcgtcggggt cgaacgacga
     1921 cagcgggccg gcgacgtaga acgggatgcc gctggcccgg gcgagcaccg cgaggttgta
     1981 ggtgccgatc ttgttggcgg tgtcgccgtt gcgggcgacc cggtcgcagc cgacgagaac
     2041 gctgtcgacg aggccctggg ccatggctgc gccggtggcg gcgtcgggca ggagcgtgac
     2101 cgggatcccg gcgtcgacca gctcccaggc ggtcagccgc gagccctgca gcagcggcct
     2161 cgtctccgtc gcccagacct ggacagggtg gcgcgccgcg gccttggcgt agacgacgcc
     2221 gagcgcggtc cccaaccccg cggtcgcgag ccgtccggtg ttgcagtggg tgaggacgcg
     2281 gtggcgggac gcgagctcgg cgcgaccatg ctcgccgatg cggcggcacg ccgcgcggtc
     2341 ctcctcgagg atgcgggtcg cctcctcgag ggccagccgc cgcatggccg cggggtcggt
     2401 gccggctcct gccgcggcgg cgacccggcg gaccgcccag cgcaggttca ccgccgtcgg
     2461 ccgggccgcc tccagctcgg cggcgacccg cccgagcccc gccctggcct cggcgacgtc
     2521 gcgcccgccg cgctcgtcga gaccgaccac gaccccgaac gccgcgcaga ccccgatcgc
     2581 cggcgccccc cgcaccacca gccggcgcag cgcgtccacc acctcggcca ccgaggcgag
     2641 ccggaggacc gacaggcgct cgggcagcgc cgtctggtcg accacctcc
//
LOCUS       DASPYY010000699         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1240390, whole genome shotgun sequence.
ACCESSION   DASPYY010000699 DASPYY010000000
VERSION     DASPYY010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240390"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VEP73_10985"
     CDS             complement(<1..1153)
                     /locus_tag="VEP73_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYT26995.1"
                     /translation="MPPRTTVAAQEPRASPPRPGDRLARGAAAAAVLALSAAAAFAVS
                     HRPLWALAAGLLAVVVAAAAARLPFAVAILVASFYFDSYLAVGVGVVTVGKLLGLLAA
                     AAWFLRWSVARRPVAGDPLLWPLAGLAAWIPLSVSTAYDQPAGLTAALRYLTFFTLAF
                     LVVQAVDGDRRTATRMVDVAVAAGAASALVGLYNFFSVSGDGRAHGPLSDPNDFAFVL
                     AVTVPLALHRARSAARPLHRALAALALAAMLAAILASFSRSALVGLAVAGAWAAATRR
                     LPLRSALLAVAGLLAVALVSFLLQPERVESALLEKRHVAQSNIDTRLVAWRVALEEFG
                     SSPVLGVGPGNFEVRFDEFELPPDAQDGPLATHNAYLSVLAELGAPGLAL"
     gene            1275..>2394
                     /locus_tag="VEP73_10990"
     CDS             1275..>2394
                     /locus_tag="VEP73_10990"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HYT26996.1"
                     /translation="MDRAEFLRVIARRKLVAIVTAVLVLAGGLLFLALEPRVYESSAS
                     VALLPVARQPDTLGAYDAIVTRLLPLYASKVDSQTFLDRVATRLPERLDGKDLKGRVF
                     AKPDPGAAVLKLVARANDPQQAARRARATLQEFLFELRGTRIIGFEVIDQPRVPDAPV
                     SPRPLLVLASSLLLAAFLAAAVAVAWERLFGRIRDLEELEAASGVPVLGAVPNERRLR
                     SFPRALFVGDPSMGVVEEGLRDIRAVLLGPGRGAPTFQKITLTSLSPEEGKSTVAANL
                     AVVIAEVGLHVLVVDADTHRPRQHEIFGVPNEQGLSTAVDVETVLQPTRFPKVSILTA
                     GPPLQRRSEIVEQYVHVVPKLNEFADVVLIDSAPLGAVA"
BASE COUNT          342 a          863 c          911 g          278 t
ORIGIN      
        1 acagggcgag gccgggcgcg cccagctcgg cgaggacgct caggtaggcg ttgtgggtgg
       61 cgagcgggcc atcctgggcg tcgggaggca gctcgaactc gtcgaacctg acctcgaagt
      121 tgcccggtcc gacgccgagc accggggagc tcccgaactc ctcgagcgca actcgccagg
      181 cgaccaggcg cgtgtcgatg ttgctctgtg ccacgtgccg cttctcgagc agggcgctct
      241 ccacgcgctc cggctgcagg aggaaggaga ccagcgcgac cgcgagcagg ccggccacgg
      301 ccaggagggc cgaccgcagc ggcaggcgcc gggtggccgc cgcccacgcg cccgccaccg
      361 ccagcccgac cagcgccgac ctcgagaagg aggcgaggat ggccgcgagc atggcggcga
      421 gcgcgagcgc ggcgagcgcc cggtggagcg gcctggcggc cgagcgggcg cggtgcaggg
      481 ccagcggcac ggtgaccgcg agcacgaagg cgaagtcgtt ggggtcgctg agcgggccgt
      541 gggcgcgccc gtcgcctgag acggagaaga agttgtacag ccccacgagc gcggacgccg
      601 cgccggcggc caccgccacg tcgaccatgc gggtcgcggt gcgccggtcg ccgtcgaccg
      661 cctggacgac caggaaggcg agcgtgaaga aggtgaggta ccgcagtgcc gcggtgagcc
      721 cggcgggctg gtcgtaggcg gtggacaccg acagcgggat ccaggcggcc agcccggcaa
      781 ggggccacag cagcgggtcg ccggccaccg ggcggcgggc gacgctccac cggaggaacc
      841 aggccgcggc cgcgaggagg ccgagcagct tgcccacggt gacgacgccc acgccgacgg
      901 cgaggtagga gtcgaagtag aacgacgcca ccaggatcgc gacggcgaac gggagcctcg
      961 ccgcggcggc cgccacgacc accgccagga gcccggcggc gagcgcccag agcggccggt
     1021 ggctcacggc gaacgccgcg gcggccgaga gcgcgagcac ggccgcggcg gcggcgccac
     1081 gggccagcct gtcccctggc cggggcgggc tcgcccgcgg ctcctgggcg gctactgtcg
     1141 ttcggggcgg catcctccgg catcccccgc atgcagccgg gtcgggacca cggcattcta
     1201 gtcgggacag cctcccaccc cggcacgacc tggacatatc ggcagggcgg gcggctccga
     1261 cctatgatgg caccgtggac agggcagagt tcctccgcgt gatcgcgcgc cggaagctgg
     1321 tcgcgatcgt caccgccgtg ctcgtcctcg ccggaggcct gctcttcctg gccctcgagc
     1381 cccgtgtcta cgagtcgagc gcctcggtcg cgctgctgcc ggtggccagg cagcccgaca
     1441 cgctcggcgc gtacgacgcg atcgtgacca ggctgctgcc gctgtacgcc agcaaggtgg
     1501 acagccagac cttcctcgac cgggtcgcca cgcggctgcc cgagcggctc gacggcaagg
     1561 acctcaaggg cagggtgttc gccaagccgg atcccggcgc cgccgtgctc aagctcgtgg
     1621 cccgcgccaa cgacccgcag caggccgccc ggcgggcgcg ggcgaccctc caggagttcc
     1681 tgttcgagct gcggggcacg cgcatcatcg gcttcgaggt catcgaccag cccagggtgc
     1741 ccgacgcccc ggtcagcccc cggcccttgc tggtcctggc gtcgtcgctg ctgctggccg
     1801 ccttcctcgc cgcggcggtg gcggtggcct gggagcgcct gttcggtcgg atccgcgatc
     1861 tggaggagct ggaggcggcc tccggggtgc ccgtcctcgg cgccgttccc aacgaacggc
     1921 ggctcaggag cttcccgaga gcgctgttcg tcggcgaccc ctccatgggg gtcgtcgagg
     1981 agggcctgcg cgacatccgg gcggtgctgc tcggcccggg ccggggcgcg ccgacgttcc
     2041 agaagatcac gctgacgagc ttgagcccgg aggaggggaa gtcgacggtc gcggccaacc
     2101 tggccgtggt catcgccgag gtcgggctgc acgtgctcgt cgtggacgcc gacacccacc
     2161 gtccgcgcca gcacgagatc ttcggcgtgc ccaacgagca gggcctcagc acggccgtcg
     2221 acgtcgagac ggtcttgcag cccacccgct tccccaaggt cagcatcctc acggccggcc
     2281 cgccgctgca gcggcggagc gagatcgtgg agcagtacgt gcacgtggtg cccaagctga
     2341 acgagttcgc ggacgtcgtc ctcatcgaca gcgcgccgct gggggccgtc gccg
//
LOCUS       DASPYY010000700         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_393642, whole genome shotgun sequence.
ACCESSION   DASPYY010000700 DASPYY010000000
VERSION     DASPYY010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393642"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1038
                     /gene="coaA"
                     /locus_tag="VEP73_10995"
     CDS             82..1038
                     /gene="coaA"
                     /locus_tag="VEP73_10995"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HYT26997.1"
                     /translation="MARPAPTDERDPLAPYLEFDRARWAALRAATPLTLTEDDLAALR
                     GVNERVSLAEVVDVHLPLSRLLDLRIRAHRALGDATARFLGDGGPPAPYVVAIGGSVA
                     VGKSTVARIVQALLARWPARPRVDLVTTDGFLYPNRVLEERGLLARKGFPESYDLPRL
                     VRFLAEVKAGRPSVAAPVYSHLAYDVLPGEAQLVERPDVLVVEGLNVLQAPRPGMSRL
                     AVSDFFDFSIYVDADEADIARWYVERFLTLRATAFRAPGSYFHRYAVLDDAEARATAA
                     GVWRTINAVNLAENIRPTRARASLVLEKGPDHLVRRILLRRR"
     gene            complement(1060..>1766)
                     /locus_tag="VEP73_11000"
     CDS             complement(1060..>1766)
                     /locus_tag="VEP73_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYT26998.1"
                     /translation="LGLGGVTGAPDPTTAYGPFYVEAFKNLVGSGFGGVALVLLIASL
                     ILSMNSATADGGRALYGIARDGMTIRQLYHLNRFHVPARAMTVDMVVNVGLILFVANN
                     LAILYMSNIGYVFSHVLALSAFLLLRKDRPGWPRPIKVGPVWVALAGVFFVLNLMFVI
                     FGVTQQKLAALLGGYKFGGYPFYLGVGVLVLSVLLYFYRRAVQDRMPITFRDKDAPTM
                     PNAEQMALLQAEEART"
BASE COUNT          248 a          678 c          601 g          239 t
ORIGIN      
        1 cccgcccggg accggctccc gtacgcttcc cacccgggcg gcgcccgccg cccggccacg
       61 tcgcacacgg gaggatggga catggcccgg ccggccccca cggacgagcg ggaccccctc
      121 gcgccgtacc tcgagttcga ccgcgccagg tgggcggcgc tgcgggccgc gaccccgctg
      181 accctcaccg aggacgacct ggccgccctg cggggggtga acgagcgggt gtcgctcgcc
      241 gaggtcgtgg acgtccacct accgctctcg cgcctgctcg acctgcgcat ccgcgcccac
      301 cgggcgctgg gggacgcgac cgcgcgcttc ctcggcgacg gcggtccgcc cgcgccgtac
      361 gtcgtcgcga tcggcggcag cgtcgcggtc ggcaagagca cggtcgcgcg catcgtgcag
      421 gcgctgctcg cccgctggcc cgcccgcccg cgcgtggacc tggtgacgac cgacggcttc
      481 ctgtacccga accgggtgct cgaggagcgc ggcctcctgg ccaggaaggg cttcccggag
      541 tcctacgacc tgccccggct ggtgcgcttc ctcgccgagg tgaaggccgg ccggccgagc
      601 gtggccgcgc ccgtctactc gcacctcgcc tacgacgtgc tcccgggcga ggcgcagctc
      661 gtcgagcggc cggacgtgct cgtcgtcgag ggcctcaacg tcctgcaggc gcctcggccc
      721 ggcatgtccc ggctcgcggt gtcggacttc ttcgacttct ccatctacgt cgacgccgac
      781 gaggccgaca tcgcccgctg gtacgtcgag cggttcctca ccctgcgggc gaccgccttc
      841 cgggcccccg ggtcgtactt ccaccgctac gccgtgctcg acgacgccga ggcccgcgcg
      901 acggcggcgg gcgtctggcg gaccatcaac gcggtcaacc tggccgagaa catccggccg
      961 acccgtgcgc gcgcctccct cgtcctcgag aaggggcccg accacctcgt ccggcggatc
     1021 ctgctccgcc gccgctgacc ccggggacgc gcccgccgct caggttctgg cctcctcggc
     1081 ctggagaagc gccatctgct cggcgttcgg catcgtgggc gcgtccttgt cgcggaacgt
     1141 gatcggcatc cggtcctgca ccgccctccg gtagaagtac agcagcaccg acagcaccag
     1201 gacgccgacg cccaggtaga aggggtaccc gccgaacttg tagccgccga gcagcgccgc
     1261 cagcttctgc tgggtcacgc cgaagatcac gaacatgagg ttcaggacga agaagacgcc
     1321 ggccagcgcc acccacaccg gcccgacctt gatggggcgc ggccatcccg gccggtcctt
     1381 ccgcagcagc aggaaggccg acagcgccag cacgtgcgag aacacgtagc cgatgttcga
     1441 catgtacagg atcgccaggt tgttggcgac gaacaggatc aggccgacgt tgaccaccat
     1501 gtccacggtc atggcccggg ccggcacgtg gaacctgttc aggtggtaga gctgcctgat
     1561 cgtcatcccg tccctggcga tcccgtacag cgcccggccg ccatccgccg tggccgagtt
     1621 catcgacagg atgaggctgg cgatcaggag cacgagcgcg acgccgccga agccggatcc
     1681 gacgaggttc ttgaacgcct cgacgtagaa ggggccgtag gcggtcgtgg gatccggcgc
     1741 gccggtcacc ccccccagcc cgagcg
//
LOCUS       DASPYY010000701         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1379696, whole genome shotgun sequence.
ACCESSION   DASPYY010000701 DASPYY010000000
VERSION     DASPYY010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379696"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VEP73_11005"
     CDS             complement(<1..350)
                     /locus_tag="VEP73_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYT26999.1"
                     /translation="MRAIQQSFHDLGAPLIDVEFVVLDLETTGGSHVHDRITEVGAVK
                     VRGGEVTGTFQTLVDPEIPIPPTITALTGISDRMVAAAEPIEVVLPCLLEFLGDAVFV
                     AHNAVFDWRFVQAAL"
     gene            complement(458..1228)
                     /locus_tag="VEP73_11010"
     CDS             complement(458..1228)
                     /locus_tag="VEP73_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27000.1"
                     /translation="MSAGDLVEAGRNHVATGEIVEAMICFNRAADDPELGAEALSLLA
                     AAYLLPGALHPGLALAVAHRAVRHPAVGPECLTRCAAVALSAGDPAFAEELAGMAVAV
                     TVHGEGALPILATARLRRGDRAGVRRLLSGIDRATGPAVFWQRLVAEASTKGWVREAM
                     GARWAMRRAGLPAPGRDYILIRAVPPVLHVACLAGTMMLALAGANQTLAELSLAMLLG
                     LSGVAVRADVTDQRFGSAAAGVAWMAAASAASLLLWSA"
     gene            complement(1301..2227)
                     /gene="fabD"
                     /locus_tag="VEP73_11015"
     CDS             complement(1301..2227)
                     /gene="fabD"
                     /locus_tag="VEP73_11015"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYT27001.1"
                     /translation="MEHKTAFTFPGQGRVAKGSGRPWAEDPAGWPFRRASERLGWDVL
                     EAIDAGDKALADTRTAQPAIYTHSAACYLLARAEGFRPDLVAGHSLGEYAALLAAGGL
                     TFEQGLDLVVRRAELAHEAGGAGAMRVVLGLPDKAVRGLCRSVRRQAGKVFLANSNAP
                     GQVVISGEPSAVEEAGRLALLAGAKRVLPLPIGGGFHTPLMNAARRPFREAIDRIQLP
                     RLEVPLVSNAKAKVVRSGRRAGRLLARQLTSPVDWTGCVERLRAMGTTRYVELGPGKV
                     LEGLIRRVDPRAVVMIASTPEEVRALAVGARS"
     gene            2384..2659
                     /locus_tag="VEP73_11020"
     CDS             2384..2659
                     /locus_tag="VEP73_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27002.1"
                     /translation="MPEHPDERFALPAAGERLRCAACGNLTRFDVVASRRTAAFWHYS
                     VAGELTVEEERDLDAVAETVRCRWCGSATQVEVVPALGHAGQERADP"
     gene            2656..>4084
                     /locus_tag="VEP73_11025"
     CDS             2656..>4084
                     /locus_tag="VEP73_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYT27003.1"
                     /translation="MSERATGSRELGDGLAPPAAPAEAGTPAAPAGEGPTSLPDVPEA
                     LLAGYLDAALAALRATPAAELPGALRQYQSWTPRRLRHPRVLGLVRRSLDTDAGFRAA
                     VDRRVLEEEEALARLVRSGRHADALASGEAPEAVARVAVALGREGEAAVHAAVEAAVA
                     DQARAQAARAESALAEAESELDSARARAESEAAAARAAREDARATRDELRRADRQVQA
                     LTERAEALEQQLPRARAAVQSARAEAAAEQRRLNGRLAELQARLAESQRQYRALRRSA
                     NRVDPVVAEAVGMLERDLAALQRATGVTEDTPVASLVPPTSLSPSARRGVPPSRPPRR
                     EPLPVPGGRNADDPETLAAWMAAPGVLVLVDGYNVTKHERGFPDRSLEDQRTLLVDLC
                     RRLARRWGPQVTVVFDGAEIAPLPTKVRLEGVGVVFTDAGRIADDEIVARVNAEPPER
                     PVVVVSSDNELRERAAALGASVTRSP"
BASE COUNT          519 a         1521 c         1591 g          453 t
ORIGIN      
        1 agcgcggctt ggacgaagcg ccagtcgaag accgcgttgt gggcgacgaa gacggcgtcg
       61 ccgaggaact cgagcaggca ggggagcacg acctcgatcg gctcggcggc ggccaccata
      121 cggtcgctga tgcccgtcag cgcggtgatc gtcggcggga tcgggatctc ggggtccacc
      181 agggtctgga acgtcccggt cacctcgccg ccgcgaacct tgaccgcgcc gacctccgtg
      241 atccggtcgt ggacgtggga gccgcccgtg gtctcgaggt cgagcaccac gaactccacg
      301 tcgatgagcg gcgccccgag gtcgtggaag ctctgctgga tcgcccgcac ccggtgtccc
      361 tcccgtctcg tccgccggga gagaacttaa gtgaacgtgt gttcgagatc aagcgccggg
      421 cggggctggg gatgcggccg gcctcccgag cggcggctca ggcgctccag aggaggagcg
      481 aggctgccga ggcagccgcc atccaggcca cgccggccgc ggccgagccg aaccgctggt
      541 cggtcacgtc ggcgcgcacg gccacgccgc tgagcccgag cagcatggcg agcgacagct
      601 cggcgagcgt ctggttcgcc ccggcgaggg ccagcatcat cgtcccggcg aggcaggcga
      661 cgtggagcac cggcggcacg gcccggatca ggatgtagtc gcggccgggg gcgggcaggc
      721 cagcccgccg catcgcccac cgcgcgccca tggcctcgcg gacccagccc ttggtggacg
      781 cctccgcgac cagtcgctgc cagaacacgg ccgggcccgt cgctcggtcg atcccggaga
      841 gcaggcggcg gacccccgcc cggtcgcccc ggcggagccg ggcggtggcc aggatcggga
      901 gcgccccctc gccgtggacg gtgaccgcca cggccatccc ggccagctcc tcggcgaacg
      961 ccgggtcgcc ggccgagagg gcgaccgcgg cgcacctggt caggcactcg gggccgacgg
     1021 ccgggtggcg gacggcccgg tgggcgacgg ccagcgcgag cccggggtgg agggcgcccg
     1081 gcaggaggta ggcggcagcc agcaggctga gcgcctcggc ccccagctcc gggtcgtccg
     1141 cggcccggtt gaagcagatc atcgcctcga cgatctcacc ggtcgcgacg tggttgcgtc
     1201 ccgcctcgac aaggtcgcct gcgctcacga gagcctccag agggctgcac cgccgcccgc
     1261 cggcgcacgt ccggtgaccg gtcagggcgt tcgtcgttcg tcaggagcgg gcgccgacgg
     1321 ccagggctcg cacctcttcg ggtgtggagg cgatcatcac gactgcccgc gggtcgacgc
     1381 ggcggatcag cccctcgagc accttgcccg gcccgagctc gacataccgg gtcgtcccca
     1441 tcgcccggag gcgctcgacg cagccggtcc agtcgaccgg ggaggtgagc tggcgggcga
     1501 gcagacggcc ggcacgcctg ccgctgcgga cgaccttggc cttggcgttg gagaccaggg
     1561 ggacctcgag gcgggggagc tggatacggt cgatcgcctc ccggaacggg cggcgggcgg
     1621 cgttcatcag cggggtgtgg aagcccccgc cgatcgggag ggggagcacg cgcttggcgc
     1681 cggcgaggag cgcgaggcgc ccggcctcct cgacggcgct gggctcgccg gagatgacca
     1741 cctggccggg ggcgttggag ttggccagga agaccttgcc ggcctgccgg cgcacgctgc
     1801 ggcagaggcc gcggacggcc ttgtccggga ggccgaggac cacgcgcatg gcgccggccc
     1861 ctccggcctc gtgggccagc tcggcccggc gcaccaccag gtcgagcccc tgctcgaagg
     1921 tcaggccgcc ggccgcgagc agggcggcgt actcgccgag ggagtggccc gcgacgaggt
     1981 cgggacggaa cccttccgcc ctcgcgagca ggtagcaggc cgcggagtgg gtgtagatgg
     2041 ccggctgtgc cgtgcgcgtg tcggccagcg ccttgtcgcc ggcgtcgatg gcctcgagga
     2101 cgtcccagcc gagccgctcg gaggcgcgcc ggaacggcca gcccgccggg tcctctgccc
     2161 agggcctgcc gctgcccttg gcgacccgtc cctggccggg gaaggtgaac gcggtcttgt
     2221 gctccacgcc agtcatcgta cgcgctcggc cgcgggcgac aaacaacgcc tcgaaccgct
     2281 cgaccccaac cgcagggtcg gccctccacc cagcctccac cccttggtcg ccaggcacgg
     2341 ttaccctggt cgtgagagcc cgatcgaccc atgcggaggc agcgtgcccg agcaccccga
     2401 cgaacggttc gcccttcccg ccgccggcga gcggctccgc tgcgccgcct gcggcaacct
     2461 gacgcgcttc gacgtggtgg cgagccgcag gacggccgcg ttctggcact actcggtggc
     2521 gggggagctg accgtcgagg aggagcgcga cctcgacgcg gtcgccgaga cggtgcgctg
     2581 ccgctggtgc ggctcggcga cgcaggtcga ggtggtcccg gcgctcgggc acgccggcca
     2641 ggaacgggcc gacccatgag cgagcgggcg accgggtcgc gcgagctggg cgacggcctc
     2701 gccccgcccg cggccccggc ggaggcgggg acgcccgcgg ccccggcggg ggagggcccg
     2761 acgtcgctgc ccgacgtccc cgaggcgctg ctcgccggct acctggacgc cgcgctggcc
     2821 gcgctgcgcg cgacacccgc cgccgagctg cccggtgcgc tgcggcagta ccagagctgg
     2881 acgccgcgcc ggctcaggca cccacgggtg ctcgggctcg tcaggcgcag cctcgacacc
     2941 gacgccggct tccgggcggc cgtggaccgg cgggtcctcg aggaggagga ggcgctcgcc
     3001 cgcctcgttc ggtccggccg gcacgccgac gcgctcgcga gcggcgaggc accagaggcg
     3061 gtggccaggg tcgccgtcgc gctcgggcgc gagggcgagg cggccgtgca tgccgcggtc
     3121 gaggcggccg tcgccgacca ggcgagagcc caggcggccc gtgccgagtc ggcccttgcc
     3181 gaggccgaga gcgagctgga ctcggcccgg gcccgggccg agtcggaggc ggccgccgcc
     3241 cgcgccgccc gcgaggacgc ccgcgcgacc cgcgacgagc tgcgccgggc cgaccggcag
     3301 gtccaggcgc tgaccgagcg tgccgaggcc ctcgagcagc agctgcccag ggcgcgggcg
     3361 gcagtgcagt ccgcccgggc cgaggcggcc gccgagcagc gccgcctgaa cggccggctg
     3421 gccgagctgc aggcccggct ggccgagagc cagcgccagt accgcgccct gcgccgctcc
     3481 gccaaccggg tcgacccggt ggtggccgag gccgtcggca tgctggagcg cgacctggcc
     3541 gcgctccagc gggcgaccgg ggtcaccgag gacacgcccg tggccagcct ggtcccgccg
     3601 acttccctgt ccccttcggc acgccgaggc gtgcctccga gccggccgcc tcgccgcgag
     3661 ccgctgcccg tcccgggcgg gcgcaacgcc gacgacccgg agacgctcgc ggcgtggatg
     3721 gccgcgccgg gcgtgctcgt gctggtggac ggctacaacg tgacgaagca cgagcgcggc
     3781 ttccccgacc gcagcctgga ggaccagcgc acgctgctcg tcgacctctg ccgccgcctc
     3841 gccaggcgct gggggccgca ggtcacggtg gtgttcgacg gcgccgaaat cgctccgctg
     3901 cccaccaagg tccggctgga gggcgtcggg gtcgtgttca ccgacgcggg ccggatcgcc
     3961 gacgacgaga tcgtcgccag ggtgaacgcg gaaccgcccg agcgtccggt ggtcgtcgtg
     4021 tcgtcagaca acgagctgcg ggagcgggcc gccgcgcttg gcgcgagcgt gacccgctcg
     4081 cccg
//
LOCUS       DASPYY010000702         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_113151, whole genome shotgun sequence.
ACCESSION   DASPYY010000702 DASPYY010000000
VERSION     DASPYY010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113151"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VEP73_11030"
     CDS             complement(<1..1710)
                     /locus_tag="VEP73_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HYT27004.1"
                     /translation="MVRLGDLGPVPSWFVVLPDDQAAGPAATVVRDHAEQQISHASGR
                     PWLLGCWAEGTVTTGRAGDVTIAVIGQHAVTADQLARAAGRIRVAADLDRLAASLVGS
                     SHLVASVAGRVRVQGTVTGVRRVFHATVDGAGVAADRADVLARLLDAGLDQQHLALHL
                     LEPQILYPFAGQPVWQGVALLPTDHYLVLDRDGRRRSVRWWAPPEPVIPMAEGAPALR
                     EALSAAVAARTRGRALVGCDLGGLDSTAICSLAARGEAAVVAYTAASLDPLADDVAWA
                     GRTVAGLGNVEHHVIPAEEMPLVYHGLLAMDDRLDEPCAATADRDRWLVIAQRAAARG
                     SGLHLTGFGGDELLYGSLAHLHPMLRTDPRTALRHLRGFAAKYRWPRKRMLRQLSDTS
                     PYPEWLLRVADTLTATRPPIEEPLLDWGFTPRLPPWATPAAVQAVGDLIRAEARATEP
                     LAKGRGQHRELETMRFVSRIARQLDQMAARIGVTLAAPYYDDRVIEAGLAVRPQERIT
                     PWRYKPLIVEAMRGIVPDQSRTRQTKANGSCDLDPGLRRHRGELLALWEDSRLHRLGL
                     VDAD"
     gene            complement(1903..2013)
                     /locus_tag="VEP73_11035"
     CDS             complement(1903..2013)
                     /locus_tag="VEP73_11035"
                     /inference="COORDINATES: protein motif:HMM:NF039305.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="keywimysin-related RiPP"
                     /protein_id="HYT27005.1"
                     /translation="MRTYERPTLTAAGSFKKLTGLSGHGPRDVLLKHQLL"
     gene            complement(2212..>2508)
                     /locus_tag="VEP73_11040"
     CDS             complement(2212..>2508)
                     /locus_tag="VEP73_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27006.1"
                     /translation="GWPRGHALLRPGRRRGPTDAEGTLRTVSATYQPHGPNCSEGGQR
                     DATGGTVTYTVVSDTLVEGGYDLEFRGDRVAGTFSAPMCVLCAPRPTSRTCLQP"
BASE COUNT          333 a          919 c          941 g          315 t
ORIGIN      
        1 gtccgcgtcg accaggccga gccgatgaag ccgggagtcc tcccacagcg ccaggagctc
       61 cccacggtgc cgtcgcagcc ccgggtccag gtcgcacgag ccgttggcct tggtctggcg
      121 ggtccggctc tggtcgggga cgatgccacg catcgcctcg acgatcagcg gcttgtaccg
      181 ccacggcgtg atccgctcct gcggccgcac cgcgaggccc gcctcgatca cccggtcgtc
      241 gtagtagggg gcggcgagcg tgaccccgat ccgggcggcc atctggtcga gctggcgggc
      301 gatgcgggac acgaaccgca tggtctcgag ctcccggtgc tggccccggc ccttcgccag
      361 cggctcggtg gcgcgggcct cggcgcggat caggtccccc accgcctgca ccgcggccgg
      421 cgtcgcccag ggtggcaggc ggggcgtgaa cccccagtcg agcagcggct cctcgatcgg
      481 gggccgggtc gcggtgaggg tgtcggcgac gcgaaggagc cactcggggt aggggctggt
      541 gtccgagagc tgccgcagca tccgcttccg tggccagcga tacttggcgg cgaagccacg
      601 caggtgccgc agggcggtcc tggggtcggt ccgcagcatc gggtgcaggt gggccaacga
      661 gccgtacagc agctcgtccc cgccgaagcc ggtcaggtgc agccccgaac cgcgtgccgc
      721 ggcccgctgg gcgatgacca gccagcggtc gcggtccgcg gtggcggcgc acggctcgtc
      781 caaccggtcg tccatggcga ggagaccgtg gtacaccagc ggcatctcct cggccgggat
      841 gacgtggtgc tcgacgttgc cgagcccggc gaccgtgcgg ccggcccagg ccacgtcgtc
      901 ggcgagggga tcgaggctcg ccgcggtgta ggcgacgacc gccgcctccc cgcgggcggc
      961 cagcgagcag atcgcggtcg agtccagccc gcccaggtcg cagccgacca gcgcgcggcc
     1021 ccgggtgcgg gcggcgaccg ccgccgacag cgcctcccgc agcgccggtg cgccctcggc
     1081 catgggtatg actggctcgg ggggcgccca ccagcggacc gaccgccgcc gcccgtcacg
     1141 gtcgagcacc aggtagtggt cggtcggcag gagtgccacc ccctgccaga ccggctggcc
     1201 ggcgaacggg tagaggatct ggggctcgag caggtggagc gccaggtgct gctggtcgag
     1261 cccggcgtcg agcaggcggg ccaggacgtc ggcccggtcc gccgcgacgc cggcgccgtc
     1321 gaccgtggcg tggaacaccc gccggacgcc ggtgacggtc ccctgcaccc ggacccgtcc
     1381 ggccaccgag gcgacgaggt gcgagctgcc gaccaacgac gccgccagcc ggtcgaggtc
     1441 ggcggccacc cggatccggc cggcggcccg ggcgagctgg tcggccgtga ccgcgtgctg
     1501 gccgatcacc gcgatggtca cgtctcctgc ccgaccggtg gtcacggtgc cctcggccca
     1561 gcacccgagc agccacggcc gccccgatgc gtggctgatc tgctgctcgg cgtggtcacg
     1621 cacgaccgtg gcggccgggc cggcggcctg gtcgtccggc aggacgacga accaggacgg
     1681 tacgggaccc agatcgccca gtctcaccat gttcgaaacc ctcgtcgttc gagaacctcg
     1741 tttgagaacc ttcgtttgag aacctccgtc gacgttgcgg cggcaccttc gggcaccgga
     1801 ttcgggcacc ggagccggaa cagcccgggt gacgaagcgg cagcagtcgc cacccgggcg
     1861 cgtccggccc ggcggggcgg accggagatg gagcacgcac gctcagagca gctggtgctt
     1921 caagaggaca tcgcgcgggc cgtggccgct cagaccggtc agcttcttga aggagccggc
     1981 ggctgtcagc gtgggacgct cgtaggtacg catcgctaca cccccctcca ccactcggcc
     2041 cccctgcctt ccgtccggga cgcctcgtcc agacgtccgc cgccgctcta cccggcgcca
     2101 taccgggcaa acgcgagcgg tgtcgtcagg cagtcggcac tactgcgtag tccgcgcggt
     2161 ccgtggccga cggccacgag gaggggtgcg acccggcggt cgcggtcgcg gctacggctg
     2221 caggcaggtt cgcgacgtcg ggcgcggggc gcacagcacg cacatcggcg cagagaaggt
     2281 ccccgccacc ctgtcgccac ggaactcgag gtcgtagccg ccctcgacca gcgtgtcgct
     2341 gacgacggtg taggtgaccg tgccgccggt ggcgtcccgc tggccgccct cggagcagtt
     2401 ggggccgtgc ggctggtagg tggcggacac cgtccgcagc gtcccctccg cgtcggtggg
     2461 gccccgacgc cgacctggtc ggagcagggc atggccacga ggccaccc
//
LOCUS       DASPYY010000703         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_817523, whole genome shotgun sequence.
ACCESSION   DASPYY010000703 DASPYY010000000
VERSION     DASPYY010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817523"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..>1916)
                     /locus_tag="VEP73_11045"
     CDS             complement(21..>1916)
                     /locus_tag="VEP73_11045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYT27007.1"
                     /translation="GSMTTVPLIPRQVLFGNPEKVSPSISPAGTRLAWLAPVDGVLNV
                     WVGTVGEDDAKPVTDDRDRGIRAFFWAEDDRHLLYVQDRGGDENWHLYAVDLVLGGTR
                     DLTPFDGVQAQLVAAEKRFPGELLVGLNKDNEELHDVYRLDLATGDLEKVADNPGFVG
                     WLADADLRVRCGVAPTPDGGLVLMLRDGDGGDWTPALAVGQEDALGTAPVEFSADGKT
                     LLLVTSKDANAARLVRLDPADGSTSVLFEDPDHDVRDLTVNPDTREPEIVTVLRDRAQ
                     LEALDPRLAGDVERIRAIHPGDPAFLGRDHADRVWLLQFTADDGPVSYYAYDRSSGDA
                     TFLFVHRPELERYRLAKTEPFVFTARDGLTVHGYATFPPGAARSGLPAVLNVHGGPWA
                     RDTWGFNPEAQWLADRGYLCVQVNFRGSTGYGKRFLNAGNRQWGAAMHDDLVDAVRLV
                     VERGWADPARVGIYGGSYGGYAALAGAAFTPDVFRCAVDVVGPSNLITLIESVPPYWK
                     PLIAQFKTRVGDPETEADLLWSRSPLSRVDQIRIPMLIAQGANDPRVKQAESEQIVAA
                     MRGKGIPHEYLLFPDEGHGFAKPANRLRFYAHAERFLAEHLGGRYEEGPTDPAAGVPP
                     DAPPPGR"
BASE COUNT          249 a          652 c          748 g          267 t
ORIGIN      
        1 ccactggtag gccgcggggc tcaccggccg ggcggtggcg cgtctggcgg cacgcccgcg
       61 gcggggtcgg tcggcccctc ctcgtagcga ccgccgaggt gctcggcgag gaagcgctcg
      121 gcgtgggcgt agaagcgcag ccggttcgcc ggcttggcga agccgtggcc ctcgtcgggg
      181 aagagcaggt actcgtgggg gatgcccttg ccgcgcatgg cggccacgat ctgctccgac
      241 tcggcctgct tgacccgcgg gtcgttcgcg ccctgggcga tcagcatcgg gatcctgatc
      301 tggtcgaccc tggagagcgg cgaccgggac cagagcaggt ccgcctctgt ctccgggtcg
      361 cccaccctgg tcttgaactg cgcgatcagc ggcttccagt agggcggcac cgactcgatg
      421 agcgtgatga ggttcgacgg cccgaccacg tcgacggcgc agcggaacac gtcgggggtg
      481 aaggccgccc cggccagggc ggcgtagccg ccgtacgacc cgccgtagat cccgacgcga
      541 gccgggtcgg cccagccccg ctccaccacg aggcgcaccg cgtcgaccag gtcgtcgtgc
      601 atcgcggccc cccactgcct gttgccggcg ttgaggaagc gcttgccgta cccggtcgag
      661 cccctgaagt tgacctggac gcacaggtag ccgcggtcgg cgagccactg cgcctcgggg
      721 ttgaagcccc aggtgtcgcg ggcccagggg ccgccgtgga cgttcagcac cgcgggcagc
      781 cccgaccgcg ccgcccctgg cgggaaggtc gcgtagccgt ggacggtgag cccgtccctg
      841 gccgtgaaga cgaacggctc ggtcttggcg agccggtagc gctcgagctc gggacgatgg
      901 acgaacagga aggtggcgtc gccgctggag cggtcgtagg cgtagtacga caccgggccg
      961 tcgtcggccg tgaactgcag cagccagacc cggtcggcgt ggtcgcggcc gaggaaggcc
     1021 ggatcgccgg ggtggatggc gcggatccgc tcgacgtcgc ccgcgaggcg agggtcgagg
     1081 gcctcgagct gcgcccggtc gcggagcacg gtgacgatct cgggctcgcg cgtgtccggg
     1141 ttcacggtga ggtcccgcac gtcgtggtcg gggtcctcga acagcacgct ggtcgagccg
     1201 tcggccgggt ccaggcgcac cagccgggcc gcgttggcgt ccttggaggt gaccagcagg
     1261 agcgtcttgc cgtcggcgct gaactcgacc ggggccgtgc cgagggcgtc ctcctgcccg
     1321 accgccaggg ccggcgtcca gtcgccgccg tcgccgtcgc gcagcatgag gacgaggccg
     1381 ccgtcgggcg tgggcgccac gccacagcgg acgcggaggt cggcgtcggc cagccagccg
     1441 acgaagcccg ggttgtcggc caccttctcg aggtcgccgg tggccaggtc cagccggtag
     1501 acgtcgtgca gctcctcgtt gtccttgttg aggccgacga gcagctcgcc ggggaagcgc
     1561 ttctcggcgg cgaccagctg ggcctggacg ccgtcgaacg gcgtgaggtc gcgcgtgccg
     1621 ccgaggacga ggtcgacggc gtacaggtgc cagttctcgt cgccgccccg gtcctggacg
     1681 tagaggaggt ggcggtcgtc ctcggcccag aagaaggccc ggatgccgcg gtcgcggtcg
     1741 tcggtgaccg gcttcgcgtc gtcctcgccg accgtgccga cccagacgtt cagcacgccg
     1801 tcgaccgggg cgagccaggc gaggcgggta ccggccggcg agatgctcgg gctgaccttc
     1861 tcggggttgc cgaagaggac ctggcggggg atgaggggta cggtcgtcat ggaccc
//
LOCUS       DASPYY010000704         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1240907, whole genome shotgun sequence.
ACCESSION   DASPYY010000704 DASPYY010000000
VERSION     DASPYY010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240907"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VEP73_11050"
     CDS             <1..1019
                     /locus_tag="VEP73_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27008.1"
                     /translation="VLTGYVPSAELVAAVRANWCQVEAADRALERRLRPLVEIVDPYE
                     QDHGPGPARLHRRSLAALREALRRGESAYVLVPRRGAADPNAPGVRSAGQVAAELGRF
                     LGSRAAEAGAAGGGGGGGTPVWRLDREVLDPGAVPPWAGRGPGVIVGTVAGVKDHPPL
                     TGCRTVVVVGADAALGQAEVRAAEEALRTWSRAAAWCGPRDRPGRLVLQTSDPAHHAV
                     QALVRWDPGFFWRTELPRRAELGFPPARRLLLIEGPELGESAAALAAVATALGPTAEL
                     LGPAIMGRGWRIIAKVEDAEWAARALRPALAEASRSGGPRMSVDVEPLEVLSAPRRPD
                     SQVS"
     gene            1060..>1538
                     /gene="def"
                     /locus_tag="VEP73_11055"
     CDS             1060..>1538
                     /gene="def"
                     /locus_tag="VEP73_11055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYT27009.1"
                     /translation="MALLQIRFFGDPVLRQRAEPVTVFDAALARFGQDLLETMRAAPG
                     VGLAAPQVGVLRRMFAYDVGADEETGEHPYGVLVNPVITRREGEQLGEEGCLSFPGLY
                     YEVTRAMEVTVAAQDVTGQDLTVTGSGLLARCLQHEFDHLDGVLFIDHLSRGDRKRA"
BASE COUNT          168 a          584 c          582 g          204 t
ORIGIN      
        1 gtgtgctcac cgggtatgtc ccctccgccg agctggtcgc cgcggtgcgg gccaactggt
       61 gccaggtgga ggcggccgac cgcgcgctgg agcggcgcct gcggccgctg gtcgagatcg
      121 tcgaccccta cgagcaggac cacggccctg gccccgcgcg gctgcaccgc cgctcgctcg
      181 ccgcgctccg cgaggcgctg cgccgcggcg agagcgccta cgtgctggtc ccgcggcgtg
      241 gcgccgccga ccccaacgcc ccgggcgtgc gcagcgccgg gcaggtcgcc gccgagcttg
      301 gccgcttcct cggcagccgc gccgccgagg caggggccgc cggtggtggc ggtggtggcg
      361 gcacgccggt gtggcggctc gaccgcgagg tcctcgaccc cggcgcggtg ccgccctggg
      421 ccgggcgcgg gccgggcgtg atcgtcggca ccgtcgccgg ggtcaaggac cacccgccgc
      481 tgaccggctg ccgcaccgtg gtggtggttg gcgcggacgc cgctctcggc caggccgagg
      541 tccgcgccgc cgaggaggcc ctgcgcacct ggagccgcgc ggcggcctgg tgcgggcccc
      601 gcgaccggcc cggccgcctg gtgctgcaga ccagcgaccc cgcccaccac gccgtgcagg
      661 cgctggtgcg ctgggaccct ggtttcttct ggcgcaccga gctgccgcgc cgcgcggagc
      721 tgggcttccc gcccgctcgc cgcctactgc tgatcgaggg tcccgagctg ggggagagcg
      781 ccgccgccct ggccgccgtc gccacggcgc tcgggccaac ggctgagctg ctcggcccgg
      841 cgatcatggg acgcggctgg cgtatcatcg ccaaggtcga ggacgccgaa tgggcggcac
      901 gcgcgctgcg gccggccctc gccgaggcgt cccgctccgg cgggccgcgg atgtcggtcg
      961 acgtggagcc gctcgaggtg ctgagtgccc cgcgccggcc cgacagccag gtttcgtgaa
     1021 ccggtttcgt gaataggttt atgaagggga aagcaaccgt tggccctgct gcagatccgc
     1081 ttcttcggcg acccagtgct gcgccagcgc gccgagccgg tcaccgtgtt cgacgcggca
     1141 ctggcccgct tcgggcagga cctgcttgag accatgcgcg ccgcccccgg cgtcgggctc
     1201 gccgcaccgc aggtgggcgt gctgcggcgc atgttcgcct acgacgtcgg cgccgacgag
     1261 gagaccggcg agcaccccta cggcgtgctg gtcaacccgg tgatcacccg ccgcgagggg
     1321 gagcagctcg gcgaggaggg gtgcctgtcg ttccccggcc tgtactacga ggtcacccgc
     1381 gccatggagg tcaccgtcgc cgcccaggac gtgaccggcc aggaccttac cgtgaccggc
     1441 tcggggctgc tggcccgctg cctccagcac gagttcgacc acctcgacgg ggtgctgttc
     1501 atcgaccacc tctcccgtgg cgaccgcaag cgcgccat
//
LOCUS       DASPYY010000705         7412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2784553, whole genome shotgun sequence.
ACCESSION   DASPYY010000705 DASPYY010000000
VERSION     DASPYY010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784553"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VEP73_11060"
     CDS             complement(<1..1501)
                     /locus_tag="VEP73_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27010.1"
                     /translation="MEATTAARPGRAGSALGALARSWARVRLPLAAAPVGGVVAIVGG
                     YLYWTTETLVSKSQGLPLSGNLYDYGFGGVRLYVLLLGLAATVYGALVWRRPALRTDQ
                     DGRALGRIGLGLAAMPVIWLLTLEFFEGLSVRKVGPGPWVAIAGGAVTWLAARGLVGV
                     APTAARPAVWERRSFRSDLGVVVAVEVIAFVIFIYGVGVDDPGEFLGYFVGLVAVIMA
                     LGRLGVLKGLTGPFDRERRAALLVMAVLLVVFPPLQQALSGDTFWVRVAAQAGIFVVA
                     ALGLNIVVGSAGLLDLGYVAFFGIGAYMAALVGNAGLSHFSLPHVPFLLVLFVLSPVV
                     AAVFGVLLGAPTLRLRGDYLAIVTLGFGEIVRITLNNWDSVTNGPNGIAAIPAPKLFG
                     HLASDDLKLGPVTISGNAQYFYLTLVLIAIVLLVIYRLNDSRVGRAWVAIREDEVAAA
                     AMGINTTVIKLLAFGAGAFFAGLAGSVDAHFGTQVSPDSYVFQISVLILA"
     gene            complement(1501..2403)
                     /locus_tag="VEP73_11065"
     CDS             complement(1501..2403)
                     /locus_tag="VEP73_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT27011.1"
                     /translation="MAELIQPLANGITKGSYYALIALGYTMVYGIIQLINFAHGEVYM
                     LGAFIGLWTFHFGATNFGWTSGSLMALFVVLLVAVLLTPVVGMSLERLAYRPLRNAPR
                     LAPLITALGASFVLQQLVAVFYSRDFLPFPRLFPDTGFTLAGIAIKWLWVFMVVLALA
                     LMGGLGAFIRGTRLGKAMRATAEDRDTARLMGIDVDRIILLTFILGSALAGVGGVLTG
                     LYIGQVNFFMGFIAGLKAFTAAVLGGIGNITGAVLGAFLLGILESYGTHFLGGQWQDV
                     FAFGVLILVLVFRPTGLLGERVSG"
     gene            complement(2539..3753)
                     /locus_tag="VEP73_11070"
     CDS             complement(2539..3753)
                     /locus_tag="VEP73_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYT27012.1"
                     /translation="MKGRFLQVGAVGVILALAATGCSRSTGGGASSTNKTVKLGVIAP
                     LTGPLSALGLGIKNGADLAIKQANQKGTVKGYTIVLDAQDDTATAATGANAANKLASD
                     PLVAGVVGTLNSSVAQQVAPVLQKASIVEISPANTNPTLTRGKDPLKNPQRVWNTYFR
                     VCTTDSVQGPFAADYAYKQAGLKKVITVNDKKTYGQGLVAAFEGQYTTDGGQLAGHKT
                     INAGDRDFGQIAGEIAAAKPDFVYYGGEFPEAGPLSSQLHDRGFTGPLFGGDGIYDDG
                     YIKAAGAKSNGDLATSVGAPTTKLATAKQFVTDYAAAGYKEPFSAYGAFAYDAANIII
                     QGIAKGLEGGGDVKSALPKIVAAVGATQNYQGAIGTTSFDQYGDTSNKVLTVYKVTGG
                     KWADVFSGSFGQ"
     gene            complement(3998..4384)
                     /locus_tag="VEP73_11075"
     CDS             complement(3998..4384)
                     /locus_tag="VEP73_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27013.1"
                     /translation="MTTDLHHSSTAHSSTAATTAEATAARRAPHRVAVRSRRAFVRQL
                     GTTIGAGLGIALLPATAGARSRSRAPTSAEDCEILCSPYNCTSNGCCLNAHVFYCRCS
                     PYARYVCIPYRTCTSFCTHTCPTGQC"
     gene            complement(4484..5071)
                     /locus_tag="VEP73_11080"
     CDS             complement(4484..5071)
                     /locus_tag="VEP73_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27014.1"
                     /translation="MNAPWIAAFLALWLLVLALALLQLGLLRRIAPVLEAAERQLHGG
                     AVALSGLPAGAPLPDFAVETRDGEVVRASSLRGQETIWLLASPGCAPCEALAAELRDA
                     GEPALGVPLVAVADASGAGLRLPPGVVTLFQRDRAAAVALQADATPHAVAVDGDGVVV
                     ATTTPNTLDDLQALAALLTGGDPPPAAPSGAAART"
     gene            complement(5068..5667)
                     /locus_tag="VEP73_11085"
     CDS             complement(5068..5667)
                     /locus_tag="VEP73_11085"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HYT27015.1"
                     /translation="MQLFALGSRFALAVLFLTAGLAKLSAPAEFARAVANYRLLPSGP
                     AGAVATWLPRLEVAAAVLLGLGLATTLAGVVLAGLLLAFTAAVAVNLLRGREIDCGCF
                     GSSAPRRITWGTVGRNLAMAAMAGAVVGWSPAVLSLLPAWRAPGGGPVATGDALAALV
                     ASTAAVAGALVLAEALRTARAAAALARPDPAGSSGEGAR"
     gene            complement(5790..6524)
                     /locus_tag="VEP73_11090"
     CDS             complement(5790..6524)
                     /locus_tag="VEP73_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYT27016.1"
                     /translation="MRSAEEVALVRALAREGRNHCEIARMVGIPRTTIRDWLVGRTPD
                     SGRARKTCPVCAGTPAALPQLSYAYLLGLYLGDGCLSAHPRGVYRLRIACADRYPGLM
                     DQCGLAMADVLPAKVGRVRGEGCTSVGAYSKHWPCLFPQHGPGRKHERRIELVEWQRE
                     IVDADPRPLVRGLIHSDGCRVLNWVKGTPYPRYHFTNESLDIRAIFGRACDALGVEWR
                     PHNRNSLSVAKHASVAILDEFIGMKR"
     gene            6549..6634
                     /locus_tag="VEP73_11095"
     tRNA            6549..6634
                     /locus_tag="VEP73_11095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6585..6587,aa:Leu,seq:caa)
     gene            6714..>7412
                     /locus_tag="VEP73_11100"
     CDS             6714..>7412
                     /locus_tag="VEP73_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYT27017.1"
                     /translation="MLGGLVPFVVAEIERTLGGRARVLPGAERTAVRLALSGPLADLL
                     ELRTPVAAYLLASVGGRRPSALLGDERLFEQVETVRRLHPPGAFSSFRLSVPGPGSAA
                     VRRLREELAGRTGLADDPDDGELLLRVRRAHGGGAGWDLLTRLSPRPLSARAWRVWNL
                     PGALNATIAAAMVELSRPRPSDRVLNLMCGSGTLLVERLARAPAASAVGCDLDPAALR
                     GARANLAAAGLAGAA"
BASE COUNT         1068 a         2549 c         2832 g          963 t
ORIGIN      
        1 tggcgaggat cagcaccgag atctggaaga cgtaggagtc gggcgacacc tgggtgccga
       61 agtgggcgtc gaccgagccg gccaagccgg cgaagaaggc gcccgcgccg aacgcgagca
      121 gcttgatgac ggtggtgttg atccccatgg ccgcggccgc cacctcgtcc tcccggatgg
      181 ccacccaggc ccggcccacc cgggagtcgt tgagccggta gatgaccagg agcacgatcg
      241 cgatcaggac gagggtcagg tagaagtact gcgcgttgcc ggagatggtg accggcccga
      301 gcttcaggtc gtcggacgcg aggtgcccga acagcttcgg ggccgggatg gcggcgatcc
      361 cgttcgggcc gttggtgacg ctgtcccagt tgttcagggt gatgcggacg atctccccga
      421 agcccagcgt cacgatggcc aggtagtcgc cccggaggcg cagggtcggc gcgccgagca
      481 gcacgccgaa gacggccgcg acgaccgggg agaggacgaa caggacgagc aggaagggga
      541 cgtgcgggag cgagaagtgg gagagccccg cgttgccgac cagcgcggcc atgtaggcgc
      601 cgatcccgaa gaaggcgacg tacccgaggt cgagcaggcc ggccgagccg accacgatgt
      661 tcagcccgag cgcggccacc acgaagatcc cggcctgggc ggccaccctg acccagaagg
      721 tgtcgcccga cagcgcctgc tgcagcggcg ggaacaccac cagcaggacc gccatgacca
      781 ggagggcggc gcgccgctcg cgatcgaacg ggccggtcag gcccttgagc acgccgagcc
      841 ggccgagcgc catgatcacg gcgaccaggc cgacgaagta gccgaggaac tcgcccgggt
      901 cgtccacccc gacgccgtag atgaagatca cgaacgcgat cacctcgacc gcgaccacga
      961 cgccgaggtc gctccggaag gagcgccgct cccagacggc cgggcgggcc gccgtgggcg
     1021 ccaccccgac caggccgcgg gcggcgagcc aggtcacggc cccgccggcg atcgcgaccc
     1081 aggggccggg gccgaccttg cggaccgaca ggccctcgaa gaactcgagg gtgagcagcc
     1141 agatgaccgg catcgcggcc aggccgaggc cgatgcggcc gagcgcgcgc ccgtcctggt
     1201 cggtccgcag cgccggccgg cgccagacga gtgcgccgta gacggtggcg gcgagcccca
     1261 gcagcaggac gtagagccgc acgccgccga agccgtagtc gtagaggttg ccggagaggg
     1321 gcaggccctg ggacttggag acgagcgtct cggtggtcca gtacaggtag ccgcccacga
     1381 tggcgaccac gccgccgacc ggggcggcgg ccagcggcag cctgacccgg gcccagctgc
     1441 gcgccagggc cccgagggcc gagcctgccc gtccgggccg ggcggcggtc gtcgcctcca
     1501 tcagccgctc acccgctctc cgaggagccc cgtcggccgg aagaccagga cgaggatgag
     1561 gacaccgaac gcgaatacat cctgccactg cccgccgagg aagtgggtcc cgtaggactc
     1621 gaggatgccg agcaggaacg cgcccagcac ggcgccggtg atgttgccga tgccgccgag
     1681 caccgccgcc gtgaacgcct tgagcccggc gatgaacccc atgaagaagt tgacctgccc
     1741 gatgtagagg ccggtgagca cgccgccgac gccggccagg gccgacccga ggatgaacgt
     1801 cagcaggatg atccggtcga cgtcgatccc catgagccgg gccgtgtccc ggtcctcggc
     1861 ggtggcccgc atcgccttgc cgagccgggt gccccggatg aaggcgccga gcccgcccat
     1921 gagcgcgagc gcgagcacca ccatgaacac ccagagccac ttgatggcga tgccggcgag
     1981 cgtgaacccg gtgtcgggga acagccgggg gaacggcagg aagtccctgc tgtagaacac
     2041 cgccacgagc tgctggagga cgaagctcgc gccgagggcg gtgatcagcg gcgcgagcct
     2101 gggtgcgttg cggagcggcc ggtacgccag gcgctcgagg gacatgccca cgaccggcgt
     2161 gagcagcacc gccacgagca gcacgacgaa cagcgccatg agcgagccgc tggtccagcc
     2221 gaagttcgtc gcgccgaagt ggaacgtcca caggccgatg aacgcgccga gcatgtagac
     2281 ctcgccgtgg gcgaagttga tgagctggat gatgccgtac accatcgtgt agccgagcgc
     2341 gatcagggcg tagtacgagc ccttggtgat gccgttggcg agcggctgga tgagttctgc
     2401 cacgctgccc ccaggtcgag gatcaggtca gccggcgggc gggctcgccc gccggtggcg
     2461 gggacgggcg agcgggcggg ccgccgcggc ccgtggaggg ccggccggtc cgcccgctgc
     2521 gctcacccgc gcggacggct actggccgaa gctgccagag aagacgtccg cccactttcc
     2581 gccggtcacc ttgtacacgg tgagcacctt gttcgaggtg tcgccgtact ggtcgaagct
     2641 ggtggtcccg atggcgccct ggtagttctg ggtcgcgccg acggccgcga cgatcttggg
     2701 cagggcggac ttgacgtcgc ccccgccctc gagccccttg gcgatgccct ggatgatgat
     2761 gttggccgcg tcgtaggcga acgccccgta ggccgagaac ggctccttgt agccggcggc
     2821 cgcgtagtcg gtcacgaact gcttggcggt ggccagcttg gtggtcggcg cgccgaccga
     2881 cgtggccagg tcgccgttgc tcttggcgcc ggcggccttg atgtagccgt cgtcgtagat
     2941 gccgtcgccg ccgaacagcg ggccggtgaa gccgcggtcg tggagctgcg acgacagcgg
     3001 gccggcctcg gggaactcgc cgccgtagta gacgaagtcg ggcttggccg cggcgatctc
     3061 accggcgatc tggccgaagt cgcggtcgcc cgcgttgatc gtcttgtggc cggcgagctg
     3121 tccgccgtcc gtggtgtact gaccctcgaa cgccgccacg aggccttggc cgtaggtctt
     3181 cttgtcgttg acggtgatga ccttcttcag gcccgcctgc ttgtaggcgt agtcggcggc
     3241 gaaggggccc tgcaccgagt cggtcgtgca gacccggaag taggtgttcc agacccgctg
     3301 cgggttcttc agagggtcct tgccgcgggt cagggtcggg ttcgtgttgg ccggcgagat
     3361 ctcgacgatg ctcgccttct gcagcaccgg ggcgacctgc tgggcgaccg acgagttcag
     3421 cgtgccgacg acgccggcca cgagggggtc gctcgcgagc ttgttggccg cgttggcgcc
     3481 ggtggccgcg gtcgcggtgt cgtcctgggc atccaggacg atggtgtagc ccttgaccgt
     3541 gcccttctgg ttggcctgct tgatggccag gtcggcgccg ttcttgatgc cgaggccgag
     3601 cgcggacagc gggccggtga gcggcgcgat gacgccgagc ttgaccgtct tgttggtgct
     3661 gctggcgccg ccgcccgtgc tcctcgagca gccggtcgcc gcgagcgcga ggatgacacc
     3721 gacagctccc acctggagga atctgccctt caagggtcgt gcccccttcc tgttcctgcc
     3781 acgagccccg ggaccttgcg ccggcgacgg tcggggcacc cgccggggcg cagcgctgtc
     3841 cagcgacccg gccatcgtcc gacggacgtc ccaccgcagc gccgggccag gtcgcgaccg
     3901 acgcactgta tcgcactgca acggcggaga ctacgtgcaa actacgaggt gggcaccgct
     3961 gcgccggtcc cggccggccc ggcccgccgc ggtcgggtca gcactggccg gtcgggcagg
     4021 tgtgggtgca gaaggaggtg cacgtccggt acgggatgca gacgtacctg gcgtagggcg
     4081 agcaccggca gtagaagacg tgggcgttca ggcagcagcc gttgctggtg cagttgtacg
     4141 ggctgcagag gatctcgcag tcctccgcgc tggtcggtgc cctgctccgg gacctggccc
     4201 cggccgtggc cgggagcagc gcgatgccga ggcccgcgcc gatggtggtg ccgagctgcc
     4261 gcacgaacgc gcggcggctc ctcaccgcca cgcggtgcgg cgcgcggcgg gcggccgtcg
     4321 cctcggccgt ggtggcggcg gtggaggaat gggcggtgga ggaatggtgg aggtcggtgg
     4381 tcacgtgatc gcctccaggc ggttgcgcgg tgggtacggg gtctcgaccg gggtctcgcg
     4441 tcggcgtgcc gtccggcctc acctgccttc ctgctcggga gcgtcaggtg cgggcggccg
     4501 ccccgctcgg cgcggccggc ggcgggtcgc cgccggtcag cagcgcggcg agcgcctgca
     4561 ggtcgtcgag ggtgttgggc gtggtggtgg cgacgacgac gccgtcgccg tcgaccgcga
     4621 ccgcgtgggg cgtggcgtcg gcctgcaggg ccacggccgc ggcccggtcg cgctggaaga
     4681 gggtgacgac accgggcggg agccggaggc cggcgcccga ggcgtcggcg acggcgacga
     4741 gcgggacgcc gagcgccggc tcgccggcgt cccggagctc cgcggcgagc gcctcgcagg
     4801 gcgcgcagcc cgggctcgcc agcagccaga tcgtctcctg gccgcgcaag gagctggccc
     4861 ggacgacttc gccgtcgcgg gtctcgacgg cgaagtcggg caggggcgcc cccgcgggga
     4921 ggccggacag ggccaccgcg ccgccgtgga gctgccgctc ggccgcctcg aggaccgggg
     4981 cgatccggcg cagcaggccg agctgcagca gcgcgagggc gagcacgagc agccacaggg
     5041 cgaggaacgc ggcgatccac ggcgcgttca ccgcgcgccc tccccgctgg agccggccgg
     5101 gtcgggcctg gccagggcgg cggccgccct ggcggtgcgc agcgcctcgg cgagcacgag
     5161 cgcaccggcc acggccgccg tgctcgccac cagggccgcg agcgcgtcgc cggtggcgac
     5221 cgggccgccg ccgggcgccc gccatgccgg cagcagcgag agcacggccg gggaccagcc
     5281 gaccaccgcg ccggccatgg ccgccatggc gaggttgcgc ccgacggtgc cccaggtgat
     5341 ccgcctcggg gcggacgacc cgaagcagcc gcagtcgatc tcccgtcccc gcaggaggtt
     5401 gaccgccacc gcggcggtga acgcgagcag caggccggcg agcacgacgc cggcgagggt
     5461 ggtcgccagc ccgaggccaa gcaggaccgc cgcggccacc tccagccgcg ggagccaggt
     5521 cgcgacggcc ccggcgggcc cgctcggcag gaggcggtaa ttggcgacgg cgcgggcgaa
     5581 ctcggccggt gccgagagct tcgcgagccc tgcggtcagg aacaggaccg cgagcgcaaa
     5641 ccgcgatcca agggcgaaca gttgcacgat gactttcgtg ttgcaattgc ggcgagatcc
     5701 cccgctcccg ggggtgggaa attctaggac gaatccgcta tccagtcaag atggtcgatc
     5761 cacctgaatt tgtgcgttcg tgcggacagt cagcgcttca tgccgatgaa ctcgtcgagg
     5821 atcgcgacgc tcgcgtgctt cgcgacagac aggctgttcc ggttgtgcgg ccgccactcg
     5881 acgccgagcg cgtcgcacgc gcgcccgaag atggcgcgga tgtccagcga ctcgttcgtg
     5941 aagtggtacc ggggataggg cgttcccttc acccagttca gcacgcggca gccgtccgag
     6001 tggatcagcc cgcgcaccag cggcctcggg tcggcgtcga cgatctcccg ctgccactcc
     6061 accagctcga tcctccgttc gtgcttgcgc cccggaccat gctgggggaa caggcacggc
     6121 cagtgcttgg aataggcgcc gaccgaggtg caaccctccc cgcggacccg gccgaccttg
     6181 gcgggcagga cgtccgccat cgcgagcccg cactggtcca tcagcccggg atagcggtcg
     6241 gcacaggcga tgcggaggcg gtacacgccg cgcggatgag ccgacaggca tccgtcaccg
     6301 aggtagaggc ccagcaggta ggcgtaggac agctggggca acgccgcggg cgtgccagcg
     6361 cacaccgggc aggttttccg cgcccgaccg gagtctggcg ttcttcctac cagccagtca
     6421 cggatggtcg ttcgcgggat gcccaccatc cgggcgatct cgcagtggtt cctgccctcc
     6481 ctggcgaggg cacggaccag ggcgacttcc tccgcggacc gcattccggt accctcctct
     6541 ctggaaacgc ccgagtggcg aaatctaggg aaacgctgga gtctcaaaca ctcttgcccg
     6601 taagggcttg cgggttcgag tcccgcctcg ggcacgtcgt ggaagctacc aggcagcgtc
     6661 gaacgcgtgt tcgtaccgcc cacagcccta tggcccggac tatcgagctc gaggtgctcg
     6721 gcgggctcgt gccgttcgtc gtcgccgaga tcgagcggac gctcggcggc cgggccaggg
     6781 tcctgccagg tgcggagcgg acggccgtcc ggctggccct gtcggggccg ctcgcggacc
     6841 tgctcgagct gcggacgccg gtcgccgcct acctgctggc gagcgtcggc gggcggcggc
     6901 cgagcgccct gctcggcgac gagcggctgt tcgagcaggt ggagacggtg cggcggctgc
     6961 accctccggg tgcgttctcg tcgttccggc tctcggtgcc cggccctggg tcggcggcgg
     7021 tcaggcggct tcgggaggag ctggccgggc ggaccgggct cgccgacgac cccgacgacg
     7081 gggagctgct cctgcgggtc aggcgcgcgc acggcggggg ggcgggctgg gacctgctga
     7141 cccggctctc gccccggccg ctgtccgccc gggcgtggcg cgtctggaac ctgcccgggg
     7201 cgctgaacgc gacgatcgcg gcggccatgg tggagctgtc gcggccgcgg ccgtcggacc
     7261 gggtgctgaa cctcatgtgc gggtcgggca ccctgctggt cgagcggctc gctcgggcgc
     7321 ccgcggccag cgcggtcggc tgcgacctcg acccggcggc gctgcgcggc gccagggcca
     7381 acctggccgc ggcgggcctg gccggggcgg cg
//
LOCUS       DASPYY010000706         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1380198, whole genome shotgun sequence.
ACCESSION   DASPYY010000706 DASPYY010000000
VERSION     DASPYY010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380198"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..578)
                     /gene="efp"
                     /locus_tag="VEP73_11105"
     CDS             complement(18..578)
                     /gene="efp"
                     /locus_tag="VEP73_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYT27018.1"
                     /translation="MPTTNDLKNGMTLNIDGNLMSVVEFQHVKPGKGGAFVRTKLKNV
                     RTGRVLDKTFRAAEPVEVAILEKREMQYLYRDETGFHFMDTETYEQSTVDAAAVGNAA
                     NWMVDGSTPYVTFYEGTAIGVELPASVELTVAETEPGVKGDRVSGATKAATVETGSVV
                     QVPLFVETGDRIKVDTRSGAYLTRVQ"
     gene            complement(634..1779)
                     /locus_tag="VEP73_11110"
     CDS             complement(634..1779)
                     /locus_tag="VEP73_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYT27019.1"
                     /translation="MSIHPVPSGPVPSAPVPSDHRTGWAREASARRRALHALLPPADL
                     VALLVTKPVNVRYLTGFSGSYGALLLFLDRTVFFTDRRYERQAAAEVPGAEIVLVPGD
                     LIAGVVSVLGSDGDGLGFEPDGLTWAMGQRLRASLPGRAVVPAPPLVEDLRQVKDAAE
                     VAAMREAARVGTEALARLLGSLRAGATEREVAVELEVSMRRLGGDGLAFDPIVAFGEQ
                     AAEPHHRPNDRELTRGDLVKLDLGAQVGGYRSDMTRTVVLGRASERQREVYELVLRAQ
                     AAGLAALRPGVVPGEVDRACREVIAAAGLGEAFSHPTGHGVGLEIHEAPWVRPGAGGS
                     IASGTPVTVEPGVYLPGFGGVRIEDLAVVRPDGHELLTTAPKELIEL"
     gene            complement(1825..>2130)
                     /locus_tag="VEP73_11115"
     CDS             complement(1825..>2130)
                     /locus_tag="VEP73_11115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYT27020.1"
                     /translation="EGLQSDHEGAIVEALLGARAAGVAAVVMNPGALAHTSYAVRDAV
                     EAAGIPVVEVHLSNVHARERFRRHLAIAPVAAGVVAGLGPASYELGLRAAAGLVGRR"
BASE COUNT          273 a          784 c          791 g          282 t
ORIGIN      
        1 cctcagccgc cggcgcctca ttggacccgg gtcaggtagg cgccgctgcg cgtgtcgacc
       61 ttgatgcggt cgccggtctc gacgaacagc ggcacctgca cgaccgagcc ggtctccacc
      121 gtggccgcct tggtcgcccc cgagacccgg tcgcccttga cgcctggctc ggtctcggcg
      181 acggtgagct cgacgctggc cggtagctcg accccgatcg ccgtgccctc gtagaaggtc
      241 acgtagggcg tcgagccgtc gaccatccag ttcgcggcgt tcccgaccgc ggcggcgtcg
      301 accgtcgact gctcgtaggt ctcggtgtcc atgaagtgga agccggtctc gtcccgatag
      361 agatactgca tctcccgctt ctcgaggatg gccacctcca ccggctcggc ggcccggaag
      421 gtcttgtcga gcacgcgccc ggtgcgcacg ttcttcagct tggtgcggac gaaggcgccg
      481 cccttgcccg gcttcacgtg ctggaactcg acgacgctca tcaggttacc gtcgatgttc
      541 agggtcatgc cgttcttcag gtcgttggtc gttggcatcg gcgcctcctg gaagcctgcc
      601 gacggccccg gcgcgccggg gccgtccgtt ccgtcacagc tcgatgagct ccttgggtgc
      661 ggtggtgagc agctcgtgac cgtccggacg gacgaccgcg aggtcctcga tccgcacgcc
      721 tccgaagccg ggaaggtaga caccgggctc gacggtcacc ggcgtgccgc tcgcgatgct
      781 accaccggct cccgggcgta cccacggcgc ctcgtggatc tcgaggccga cgccgtggcc
      841 ggtagggtgc gagaacgcct cgccgagccc cgccgccgcg atgacctcgc ggcaggcccg
      901 gtcgacctcg ccggggacga cgccggggcg cagcgcggcc agcccggccg cctgggcgcg
      961 caggaccagc tcgtatacct cgcgctggcg ctccgaggcg cggccgagca ccaccgtgcg
     1021 ggtcatgtcg gagcggtagc cgccgacctg ggcaccgagg tcgagcttca cgaggtcgcc
     1081 cctggtgagc tcccggtcgt tcggccggtg gtggggctcg gcggcctgct ccccgaaggc
     1141 gacgatcggg tcgaaggcga ggccgtcccc gccgagccgg cgcatggaga cctcgagctc
     1201 gaccgcgacc tcgcgctcgg tcgcgcccgc ccgcagggag ccgagcagcc gggcgagcgc
     1261 ctcggtgccg acccgggcgg cctcgcgcat ggccgcgacc tcggcggcgt ccttcacctg
     1321 gcggaggtcc tcgacgaggg gcggggcggg cacgacggcg cggccaggga gcgaggcgcg
     1381 cagccgctgg cccatcgccc aggtgagccc gtcgggctcg aacccgaggc cgtcgccgtc
     1441 gctgccgagc acgctgacga cgccggcgat gaggtcgccg gggaccagca cgatctcggc
     1501 gccgggcacc tcggccgccg cctgccgctc gtagcggcgg tcggtgaaga agaccgtgcg
     1561 gtcgaggaac aggagcagcg cgccgtagct gcccgagaag cccgtgaggt agcgcacgtt
     1621 gaccggcttg gtgacgagca gcgcgaccag gtcggcgggt ggcagcaggg catgcagggc
     1681 acggcggcgg gcgctggcct cgcgcgccca gcccgtgcgg tggtccgaag ggacgggcgc
     1741 ggaggggacg gggccggagg ggacggggtg gatggacacg tcgactcctc acatctcgaa
     1801 gcgcaccggc gctccccggt ctggctaccg ccggccgacc aggccggcgg ccgcccgcag
     1861 cccgagctcg tagcttgccg gcccgagccc ggccaccacg ccggccgcca ccggcgcgat
     1921 ggccaggtgg cgccggaagc gctcgcgtgc gtgcacgttg gacaggtgga cctcgaccac
     1981 ggggatcccg gccgcctcga ccgcgtcccg cacggcgtag gaggtgtgcg cgagcgcccc
     2041 cgggttcatc accaccgccg cgacgcccgc cgcgcgcgcg ccgagcaggg cctcgacgat
     2101 tgcgccctcg tgatcggact gcaggccctc
//
LOCUS       DASPYY010000707         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1241416, whole genome shotgun sequence.
ACCESSION   DASPYY010000707 DASPYY010000000
VERSION     DASPYY010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241416"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEP73_11120"
     CDS             <1..270
                     /locus_tag="VEP73_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYT27021.1"
                     /translation="RVLVGGVVTHRQRPATAGGVTFVSLEDETGIVNVICSPGMWARY
                     RRVAQAAPALLVRGRLERAEGVANVLADQAQPLHLGMATRSRDFR"
     gene            383..730
                     /locus_tag="VEP73_11125"
     CDS             383..730
                     /locus_tag="VEP73_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27022.1"
                     /translation="MERDRDRLDAEDDVLEERLPRDTGVIGSEAEEPGPGRDTARTDP
                     DPVSESVEQPRTTVAMSEADPVRTRLGAQEEDVEPIGAAAPELARDLPGDEEDLAAER
                     PRRYGDDEDPPAT"
     gene            complement(697..>1561)
                     /locus_tag="VEP73_11130"
     CDS             complement(697..>1561)
                     /locus_tag="VEP73_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HYT27023.1"
                     /translation="SGRGPGEARWDPAAGTVDAGALAAADAVVHLAGRPIASGRWTDR
                     ARREIRDSRVQGTRLLAETMARLGGGGSAGRPRGPRVLVVSSAIGYYGDRGDEVLTEA
                     SPPGDGFLAGVCKAWEAAADPARAAGLRVVHVRTGLVLTPEGGSLPKLLPLFKLGLGG
                     RFGSGRQWWSWVALDDVLGIYRHALVTDGLHGPVNATAPGPVTNAELAATLARVLGRP
                     ALVPVPRFGPRLVLGEMADELLFSSARVQPEAALASGYAFRRPQLEPALRALLHRPAA
                     AQVAGGSSSSP"
BASE COUNT          200 a          599 c          612 g          150 t
ORIGIN      
        1 cgggtgctgg tcggcggggt ggtgacccac cggcagcggc ccgccaccgc cgggggcgtg
       61 acgttcgtga gcctcgagga cgagaccggc atcgtcaacg tcatctgctc gcccggcatg
      121 tgggcacgct accgccgggt cgcccaggcc gcgcccgccc tgctcgtccg cgggcgcctc
      181 gagcgcgccg agggcgtcgc caacgtgctc gccgaccagg cccagccgct gcacctcggg
      241 atggcgacga ggtcgaggga cttccggtga tcaccggact cgggcacccg ggcaccgccg
      301 ggccggcacg gccgggtggc cccgggacgt ttgccctgcc gcccggggag gaagccccgt
      361 ggtacctgcg agaggaggcc ccatggagcg cgaccgcgat cgtttggacg cagaggacga
      421 cgtgctggaa gagcggctgc cacgcgacac cggtgtcatc ggcagcgagg ccgaggagcc
      481 ggggccgggc cgcgacacgg ctcgtaccga ccccgacccg gtgagcgaga gcgtcgagca
      541 gccccgtacg accgttgcga tgtccgaggc cgaccccgtg cgcacccgcc tgggggcgca
      601 ggaggaggac gtggagccga tcggcgctgc agcccccgag ctggccaggg acctccccgg
      661 cgacgaggag gacctcgcgg cggagcggcc gcggcgttac ggcgacgacg aggacccgcc
      721 ggcgacctga gcggccgccg gacggtgcag cagggcccgc agggccggct ccagctgcgg
      781 ccgccggaac gcgtacccac tcgccagggc ggcctcgggc tgcacgcggg cgctggagaa
      841 gagcagctcg tcggccatct ccccgaggac cagcctcggg ccgaacctgg ggacgggcac
      901 gagcgccggc cgcccgagca cccgcgcgag ggtggcggcg agctcggcgt tcgtcaccgg
      961 gccgggcgcc gtcgcgttga ccggcccgtg gaggccgtcc gtgacgaggg cgtggcggta
     1021 gatgccgagc acgtcgtcca gcgcgaccca gctccaccac tgccggcccg acccgaaccg
     1081 ccccccgagg ccgagcttga acagcggaag cagcttgggc agcgagccgc cctcgggggt
     1141 cagcacgagc ccggtccgga cgtgcacgac ccgcaggccg gccgcccggg ccgggtcggc
     1201 ggccgcctcc cacgccttgc agacgccggc caggaagccg tcccccgggg ggctcgcctc
     1261 ggtcagcacc tcgtcgcccc ggtcgccgta gtagccgatc gccgacgaca cgacgaggac
     1321 gcgggggccg cggggccgcc cggcggagcc cccgccgccc aggcgggcca tcgtctcggc
     1381 gagcaggcgg gtgccctgga ccctgctgtc gcggatctcg cgcctggccc ggtcggtcca
     1441 gcggcccgac gcgatgggcc tgccggcgag gtgcacgacg gcgtcggcgg cggccagcgc
     1501 cccggcgtcg accgtgccgg cggccgggtc ccagcgggcc tcgcccggcc cccggccgga
     1561 c
//
LOCUS       DASPYY010000708         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1660789, whole genome shotgun sequence.
ACCESSION   DASPYY010000708 DASPYY010000000
VERSION     DASPYY010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660789"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1785)
                     /gene="polX"
                     /locus_tag="VEP73_11135"
     CDS             complement(46..1785)
                     /gene="polX"
                     /locus_tag="VEP73_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HYT27024.1"
                     /translation="MAWVNEELAAVFREFTELLELRGETGFRVRAYDRAARAMSGYDR
                     DLASLSEAELAALPGVGKAIAAKAREYLEHGHVRELEELRAEVPEGLRVLRTVPGVGP
                     RKARLIHERLGVASIEELTQAVAAGKIAGLPGMGARTEQNLRRALERLAATHGQGIPL
                     AEALPLAEWLRDQLARDAAAGVEQVELAGSLRRMKAVVHDIDLLAAGADPGAITRAFA
                     ALDGVAEVAALGDTKVTILTRQGQQVDLRVVRPEVWGAALQYFTGSKEHNVKVRELAV
                     KQGYKLSEYGLHTRGGDLVVALDEAVVYERLGLSWVPPVLREDRGEVEAARDGTLPRL
                     VALDDVGGDFHCHTSLSPDGTAPLEVMVDAARARGYRFLAITDHAERLSLGGATRADL
                     LAQRRRLRALTDDRGDIQLLHGAELNIGPDGSLDYDDDFLDGFDVLVASVHHQLDQPR
                     EVLTSRLIAACEHPAVNVIGHPTGRQLGRRAPSDVDLDALYQAAARTGTALEVNGSPH
                     RLDLSDEHARLAARCGVLLAFGSDSHGPGHLANMRFAVATAQRGWVGPQQVLNTLPEA
                     ALRRFLAKGRPGR"
     gene            complement(1792..>2267)
                     /locus_tag="VEP73_11140"
     CDS             complement(1792..>2267)
                     /locus_tag="VEP73_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27025.1"
                     /translation="STRIAWPIAAAFAGAAASTLVTAGAAAVAGRQADLALQPVELAL
                     RVGLLLGAPCVGALLAALVIPSGGGLPAIVGVGVGVPLGPLLVLRLDRMRDFGLIPTL
                     LTVGVLLLWGLLGALLVAGWWGRPDHAVERTRLPPPPLPEPGPGRGSGPDAGGVA"
BASE COUNT          287 a          772 c          931 g          277 t
ORIGIN      
        1 gcagcgccgc cagcggaagt tgaggcggga cggccgcggg cgcggtcacc gccctggccg
       61 ccctttggcg aggaagcggc gcagcgccgc ctcgggcagg gtattgagca cctgctgggg
      121 gccgacccag ccacgctggg cggtggccac ggcaaagcgc atgttggcga ggtggccggg
      181 gccgtggctg tcggagccga aagcgagcag cacgccgcag cgggccgcga gccgggcgtg
      241 ctcgtcgctg aggtcgaggc ggtggggtga gccgttcacc tccagcgcgg tgccggtgcg
      301 cgcggcagcc tggtacagcg cgtccaggtc cacgtcgctg ggggcgcggc ggccgagctg
      361 gcggccggtt gggtggccga tgacgttgac ggcgggatgc tcgcaggcgg cgatcaggcg
      421 ggaggtcagt acttcgcggg gctggtcgag ctgatggtgg acgctggcga ccaggacgtc
      481 gaagccgtcc aggaagtcgt cgtcgtagtc gaggctgccg tcggggccga tgttcagctc
      541 cgcgccgtgc agcagctgga tgtcgccacg gtcgtcggtg agggcgcgga gccgcctgcg
      601 ctgggcgagc aggtcggcgc gggtggcgcc gccgagggag agccgttcgg catggtcggt
      661 gatggccagg aagcggtagc cgcgtgcccg ggctgcgtcg accatgacct ccagtggcgc
      721 ggtgccgtcg ggtgacaggc tggtgtggca gtggaagtcg ccgccgacgt cgtcgagggc
      781 gaccagccgc ggcagggtgc cgtcccgggc cgcctcgacc tcgccgcggt cctcgcgcag
      841 caccggcggc acccagctga ggccgaggcg ctcgtagacg acggcttcgt ccagggcgac
      901 gacgaggtca ccgccgcggg tgtggaggcc gtactcgctg agcttgtagc cctgtttgac
      961 agccagctcg cggaccttga cgttatgttc cttggagccg gtgaagtact gcagcgcggc
     1021 gccccagacc tcggggcgga ccacgcgcag gtcgacctgc tggccttgcc gggtgaggat
     1081 cgtcactttg gtgtcgccga gggcggccac ctcggcgacg ccgtccagcg cggcgaaggc
     1141 gcgggtgatg gcgccggggt cggcaccggc ggcgagcagg tcgatgtcgt ggacgacggc
     1201 cttcatgcgc cgcagcgagc cggccagctc cacctgctcc accccggctg cggcgtcgcg
     1261 ggcgagctgg tcgcggagcc actcggcgag cggcagcgcc tcggccagcg ggatgccctg
     1321 gccgtgggtg gcggcgaggc gctccagggc gcggcgcagg ttttgctcgg tgcgggcgcc
     1381 catcccgggc agtccggcga tcttgccggc cgcgaccgcc tgggtgagct cctcgatgct
     1441 ggcgacgccg aggcgctcgt ggatgaggcg ggccttgcgc ggcccgaccc cggggacggt
     1501 gcgcagcacc cgcaggccct cggggacctc ggcgcggagt tcctcgagct cgcgcacgtg
     1561 gccgtgctcc aggtattcgc gcgccttggc ggcgatcgcc ttgccgacgc cggggagggc
     1621 ggccagctcc gcctcggaga ggctggcgag gtcgcggtcg tagccggaca tggcgcgggc
     1681 ggcgcggtca taggcgcgca cccggaagcc ggtctcgccg cgcagctcga gcagctcggt
     1741 gaactcgcgg aacaccgcgg cgagttcctc gttgacccag gccatccgct gctacgcaac
     1801 cccgccggcg tccggcccgg acccacgtcc ggggccgggc tctggcagcg gcggaggcgg
     1861 cagccgggtg cgctcgacgg cgtggtcggg ccgcccccac cagccggcga cgagcagggc
     1921 gcccagcagc ccccacagga gcaggacgcc gacggtgagc agggtgggga tcagaccgaa
     1981 gtcgcgcatg cggtcgagcc gcaggaccag cagggggccg agcggcacgc ccacgccgac
     2041 gccgacgatg gcggggaggc cgccgccgga cgggatcacc agcgcggcga gcaacgcgcc
     2101 gacgcagggc gcgccgagca gcagtccgac ccgcagggcc agctcgaccg gctgcagcgc
     2161 gaggtcggcc tgccgcccgg cgacggcggc ggcgccggca gtcaccaggg tgctggcggc
     2221 cgcgccagcg aacgctgccg cgatcggcca ggcgatcctg gtggagc
//
LOCUS       DASPYY010000709         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1242008, whole genome shotgun sequence.
ACCESSION   DASPYY010000709 DASPYY010000000
VERSION     DASPYY010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242008"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VEP73_11145"
     CDS             <1..161
                     /locus_tag="VEP73_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HYT27026.1"
                     /translation="LFAVHGRAGEPCFVCGDTIRSVWMGERETSYCPTCQTGGRILAD
                     RRRSRFLR"
     gene            327..1157
                     /locus_tag="VEP73_11150"
     CDS             327..1157
                     /locus_tag="VEP73_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYT27027.1"
                     /translation="MPELPEVETLRRDLEGEVVGRKVKGVDVKVPAIVRRQSPAEVAR
                     RVVGRKLAEVARRGTTLLLVLDSGGVLAIRLGATGRLLRERTAAPVERRTDAVVTFTT
                     GGDLRMLGLGADGELFVAAPGDPDADPRARPAAIDPLADAFTWQAFNAKLRSRRSKLR
                     PLVTDESFMAGIGPVYADEILWTSGLRWDRGSDSLTAQEVRRLYRAIQEVVQEALRLR
                     GTSVGDDVHVDLHGNKGEYQSQLRAYHREGQPCQRCRTPLVREQLEHASTFYCPRCQS
                     "
     gene            1198..>1568
                     /locus_tag="VEP73_11155"
     CDS             1198..>1568
                     /locus_tag="VEP73_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27028.1"
                     /translation="MAWRRPASPLAAAVLALAVTIAVAGCGRSAGHGPQAAPVTGAQA
                     GAQPSTSSRTGASQATGATAGTLPAGKVAFAALATTDVTVLSAPASNDVVALFPATQR
                     AGVPTPFLVEQQREQAGRRWI"
BASE COUNT          196 a          611 c          564 g          197 t
ORIGIN      
        1 ggctgttcgc cgtgcacgga cgcgccggcg agccgtgctt cgtgtgcggc gacacgatcc
       61 gctcggtgtg gatgggggag cgggagacct cctactgccc cacgtgccag acagggggcc
      121 gcatcctcgc cgaccgccgc cggtcgcgct tcctcaggtg acccgggcag gcggcccggg
      181 cggcgccggc ttctgcccgg acgtccgcgc cgcattgtgc acgagcgcac gagcgcacga
      241 cccgggcgcc gattacactc tgctgccatt cccggggaca cgactcgggc ggtggtgacg
      301 agcggtcgtg acgagcggag gagcccttgc cggagctgcc cgaggttgag acgctccgcc
      361 gtgacctcga gggcgaggtc gtcggcagga aggtgaaggg ggtcgacgtc aaggtccccg
      421 ccatcgtccg ccgccagagc cccgccgagg tcgccagacg ggtggtcggg cgcaagctgg
      481 ccgaggtcgc ccggcgcggc accaccctgc tgctcgtcct cgacagcggc ggcgtcctcg
      541 ccatccgtct cggcgcgacc gggcgcctgc tgcgggagcg caccgccgcc ccggtcgagc
      601 ggcgcaccga cgccgtggtc accttcacca ccggcggcga cctgcgcatg ctcggtctcg
      661 gggccgacgg cgagctgttc gtggccgcgc ccggcgaccc ggacgccgac ccgcgggccc
      721 gccccgcggc gatcgacccg ctcgccgacg ccttcacctg gcaggccttc aacgcgaagc
      781 tccgctcccg gcggtcgaag ctgcgcccgc tggtcaccga cgagtcgttc atggcgggga
      841 tcggtcccgt ctacgccgac gagatcctgt ggacgagcgg gctgcgctgg gaccgcggct
      901 ccgactcgct gacggcccag gaggtccgcc gcctgtaccg ggccatccag gaggtcgtcc
      961 aggaggcgct gcgcctgcgc gggacgtcgg tcggcgacga cgtccatgtc gaccttcatg
     1021 gcaacaaggg ggaatatcag tcccagctgc gcgcgtacca tcgtgagggg cagccctgcc
     1081 agcgctgccg cacgccgctg gtccgcgagc agctcgagca cgcgagcacc ttctactgcc
     1141 ccaggtgcca gagctgagct accgccccag atgccagagc tgagaggaga ccccgccatg
     1201 gcgtggcgcc ggcccgcgtc cccactcgcc gccgccgtcc tcgcgcttgc cgtcacgatc
     1261 gcggtcgccg gctgcggccg ctcggccggg cacggcccgc aggccgcgcc ggtcaccggc
     1321 gcccaggccg gcgcccagcc gagcaccagc tcccggacgg gcgcgtccca ggcgaccggc
     1381 gccaccgccg ggacccttcc cgccggcaag gtcgccttcg ccgcgctcgc gaccaccgac
     1441 gtgacggtct tgtcggcgcc cgcgtccaac gacgtcgtcg ccctgttccc ggcgacgcag
     1501 cgggccgggg tcccgacccc gttcctcgtc gagcagcagc gcgagcaggc gggccgccgg
     1561 tggatcca
//
LOCUS       DASPYY010000710         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_256555, whole genome shotgun sequence.
ACCESSION   DASPYY010000710 DASPYY010000000
VERSION     DASPYY010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256555"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2492)
                     /locus_tag="VEP73_11160"
     CDS             complement(<1..2492)
                     /locus_tag="VEP73_11160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT27029.1"
                     /translation="MRHPPTDEQAGIISAPMAPLLVVAGAGSGKTETMAARVVWLVAN
                     GYARPEQVLGLTFTRKAAGELAHRVRARLGQLVRKLGRDDLLSGEPTVATYHSYAARI
                     VTEHGLRSGFEPATRLLTEATCWQFADAIVRAYDGDMSAVTWAPSTVTDAVLKLAADL
                     SEHLVTADDLAAWTGRFHAEISGRPGRVHRPVEELLARQRARLQLLPLVRAYGQRKRD
                     LEAMDFGDQLARAAAVARDHAEVGAIERDRYRVVLLDEYQDTSHAQVVLLRSLFGHAH
                     PVTAVGDPCQSIYGWRGASAGTLDRFPGEFPDREGRPARQLALSTSWRNRPEILRVAN
                     ALSDPLRTHGHQVTRLTAAPVVAPRAGHATVHCVLLPGYADEAAWIAGRIAAAWTHDT
                     DADVEGVPVEDRPTTAVLIRVRAQIPAIEAALRERGLPVDVVGLGGLLDTPEVRDVVC
                     TLRILADPADGPALLRLLTGPRWRLGPRDLVALYRRAQAIAAYRRDGEALTVDRLEAA
                     ALAEALDDLGEAERYSPEGYRRFSALGNELRRLRRHLDQPLPDLIAEVERTIGLDVEV
                     AVRPGDAGLARAHLDALGETAARFASEAEGPTLSAFLAYLEAAELEERGLPAGEVEVV
                     EGAVQILTAHAAKGLEWDVVAVAGLSDGVFPGGARSDDHYLGGLGVLPFPLRGDRAGL
                     PALDLSRATDQKGVAEAVKAFKADWAAHDEREERRLGYVGVTRPRRLLLCSGYWWGEG
                     TKKPRGPSPFLTEIRAACLAGAGLVDEWTPAPAPNATNPSAAGAVTARWPADPLGDRR
                     AVLDEAAALVRAASGTGEDDDDPWAQEVELLLA"
BASE COUNT          305 a          940 c          952 g          308 t
ORIGIN      
        1 gcgagcagca gctcgacctc ctgggcccag ggatcgtcgt cgtcctcgcc ggtcccgctc
       61 gcggcgcgca ccagtgccgc ggcttcgtcg aggaccgcgc ggcggtcgcc gagcgggtcg
      121 gccggccagc gcgcggtgac cgccccggca gcgctcgggt tggtcgcgtt cggggcgggt
      181 gccggcgtcc actcgtcgac gagcccagcg ccggccaggc aggcggcccg gatctcggtg
      241 aggaacggcg acgggccgcg cggcttcttc gtaccctcgc cccaccagta ccccgagcac
      301 agcagcaggc gccggggccg ggtcacgccg acgtatccga gccggcgctc ctcccgctcg
      361 tcgtgtgcgg cccagtccgc cttgaacgcc ttgaccgcct cggcgacccc cttctggtcg
      421 gtggcccggg acaggtccag cgccggcagc ccggcccggt cgccgcgcag cgggaacggc
      481 agcacgccga gcccgcccag gtagtggtca tcggaccggg cgccgcccgg gaagaccccg
      541 tcggacaggc ccgctacggc caccacgtcc cactccaggc ccttggcggc gtgcgcggtc
      601 aggatctgta ccgcaccctc gacgacctcg acctcgcccg cgggtagccc gcgttcctcc
      661 agttcggccg cctccaggta ggcgaggaac gcgctgagcg tcggaccctc ggcctcgctg
      721 gcgaaccgcg cggcggtctc gccgagcgcg tcgaggtggg cgcgggcgag gccggcgtcg
      781 ccgggccgga cggcgacctc gacgtcgagc ccgatggtcc gctcgacctc ggcgatcagg
      841 tcgggcagcg gctggtcgag gtggcggcgc agccggcgca gctcgttgcc cagtgcgctg
      901 aaccgccggt acccctcggg cgagtaccgc tccgcctccc cgaggtcgtc gagcgcctcg
      961 gcgagggcgg ccgcctccaa ccggtcgacg gtcagcgcct cgccgtcgcg acggtaggcg
     1021 gcgatggcct gggcgcgccg gtacagcgcg acgaggtcgc gcgggcccag ccgccaacgg
     1081 gggccggtca gcagccggag cagggccggc ccgtcggccg ggtcggccag gatccgcagc
     1141 gtgcatacca cgtcgcggac ctcgggtgtg tcgagcaggc cgccgaggcc gaccacgtcg
     1201 accggcagcc cccgctcgcg cagcgccgcc tcgatcgccg gaatctgcgc gcgtacccgg
     1261 atcagcaccg ccgtcgtcgg ccggtcctcg accgggacgc cctcgacgtc ggcatctgtg
     1321 tcatgggtcc atgcggcggc gatccggccg gcgatccagg ccgcctcgtc ggcgtagccc
     1381 ggaagcagta cacagtggac ggtcgcgtgc cccgccctgg gcgccaccac gggcgcggcg
     1441 gtcagccggg tcacctggtg gccatgcgtg cgcagcgggt cggacagcgc gttggcgacc
     1501 cggaggatct ccggccggtt gcgccagctc gtggacaggg cgagctgccg ggccggccgg
     1561 ccttcccggt cggggaactc gcccgggaac cggtcgaggg taccggcgct ggcgccgcgc
     1621 cagccgtaga tcgactggca cgggtcaccg acggcggtga ccgggtgcgc gtggccgaac
     1681 agcgagcgca gcagtaccac ctgggcatgg ctggtgtcct ggtactcgtc gagcagcacg
     1741 acccggtacc ggtcgcgctc gatcgcgccg acctcggcgt ggtcgcgggc gaccgcggcg
     1801 gcccgggcga gctggtcgcc gaagtccatc gcctccagat cgcgcttgcg ctgcccgtac
     1861 gcgcggacca gcggaagcag ctggagccgg gcgcgctggc gggcgagcag ctcctcgacc
     1921 ggccggtgta cccgccccgg ccggcccgag atctccgcgt ggaaccggcc ggtccaggcc
     1981 gcgagatcgt ccgcggtaac caggtgctcg gacaggtcgg cggcgagctt gaggacggca
     2041 tcggtcaccg tgctcggcgc ccacgtgacg gcggacatgt cgccgtcgta cgcgcggacg
     2101 atcgcgtcgg cgaactgcca gcaggtcgct tcggtgagca gccgggtggc cggctcgaag
     2161 ccggagcgca gcccgtgctc ggtgacgatc cgggcggcgt acgagtggta ggtggcgacc
     2221 gtcggctcgc cgctgagcag gtcgtcgcgg cccagcttgc ggaccagctg gccgagccgg
     2281 gcccggaccc ggtgagccag ctccccggcg gccttgcggg tgaaggtgag gccgaggacc
     2341 tgctcgggcc gggcgtaccc gttggcgacc aaccacacca cgcgcgccgc catcgtctcg
     2401 gtcttgcccg atccggcgcc cgcgacgaca agcagcggcg ccatcggcgc ggagatgatc
     2461 ccggcctgct cgtcggtcgg cgggtgccgc agcccgagta gcgtc
//
LOCUS       DASPYY010000711         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1661331, whole genome shotgun sequence.
ACCESSION   DASPYY010000711 DASPYY010000000
VERSION     DASPYY010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661331"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1293
                     /locus_tag="VEP73_11165"
     CDS             157..1293
                     /locus_tag="VEP73_11165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HYT27030.1"
                     /translation="MRDLRSFLLVAGAAALVTYLMTPVVRFVALKLGAVDRPGGRKVH
                     AIPTPTLGGVAMFAGFLGGLAVAWRMPAFRPLFLASGLPGFGPVLLVSEVLGVAIGGA
                     MMVVIGMVDDTTGLTAPAKLAAQIFVASTMTLFGVRVLSFWLPVAGTVVLSPDLGVPV
                     TVLLIVITVNAVNLVDGLDGLAAGLVGIGALAYFIYSYRIGATGVIADTSPASLLSAV
                     LVGACAGFLPHNFNPARIFMGDSGSMLLGTLLSGATITGIGRSSQPQAGDQFALLIPV
                     AIPLLVLALPFLDTFLAVGRRMRSGKGIMTADKNHLHHRLLEIGHSHRKAVLVLYAWS
                     ALLASSAVALSFTGPTRVLPAFLVVVALGVVALLGPRRRLRHRV"
     gene            1470..>1884
                     /locus_tag="VEP73_11170"
     CDS             1470..>1884
                     /locus_tag="VEP73_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27031.1"
                     /translation="MTVEARIVRGAALATTLAAPVLVAVAWVAAGRAGAVGALLGVML
                     AVAFFAVTVVVVGMAAKVSDDLMLPAALGTYLLKVVGLGVVFLTLHDTTAFDRSAFAI
                     ATVAGTCVFLVAELRLAARARIPAVVTGDRDAVVTG"
BASE COUNT          188 a          734 c          639 g          323 t
ORIGIN      
        1 gcggcggtcc agctcgtcgt gggcggcctg gtccaacggc atgtgcagcg gcggagccat
       61 gatcgtctcc cttgcgatca ggagacttga ccatgcccct tctcaaccag gccacgcaac
      121 cactctcggg aagcatttag ccgatcggcg gccctggtgc gcgacctccg ctccttcctg
      181 ctcgtcgccg gggccgcggc gctcgtcacc tacctcatga cgccggtcgt ccgcttcgtc
      241 gcgctcaagc tgggcgcggt cgaccggccc ggtggccgca aggtgcatgc gatccccacc
      301 ccgaccctcg ggggggtcgc gatgttcgcc ggcttcctgg gcggccttgc ggtcgcctgg
      361 cgcatgcccg cgttccggcc gctgttcctc gcctccgggc tccccgggtt cgggcccgtg
      421 ctgctggtct ccgaggtgct cggggtcgcc atcgggggcg ctatgatggt cgtgatcggc
      481 atggtcgacg acaccaccgg cctcaccgcc ccggccaagc tggccgccca gatcttcgtg
      541 gcgagcacga tgaccctgtt cggggtgcgg gtgctgtcct tctggctgcc ggtggcgggc
      601 accgtcgtcc tctcgcccga cctcggcgtg ccggtgaccg tgctgctcat cgtgatcacc
      661 gtcaatgcgg tcaacctggt cgacgggctg gatgggctcg cggccgggct ggtcggcatc
      721 ggtgcgctcg cctacttcat ctacagctac cggatcgggg ccaccggggt catcgcggac
      781 acgtcgcccg cctcgctgct cagcgccgtg ctggttggcg cctgcgccgg cttcctgccc
      841 cacaacttca acccggcccg catcttcatg ggggactcgg gctcgatgct gctcggcacc
      901 ctgctctcgg gcgcgaccat caccggcatc gggcgctcga gccagccgca ggccggcgac
      961 cagttcgccc tgctgatccc ggtggccatc cccctgctcg tgctcgccct gcccttcctc
     1021 gacaccttcc tcgccgtcgg gcgccgcatg cggtcgggca agggcatcat gaccgcggac
     1081 aagaaccacc tgcaccaccg cctgctcgag atcggccatt cccaccgcaa ggcggtcctc
     1141 gtcctctacg cctggagcgc cctgctggcc agctcggcgg tggccctgtc gttcaccggc
     1201 ccgacccggg tgctgcccgc cttcctcgtc gtggtcgccc tcggcgtggt cgcgctgctc
     1261 ggcccgcgcc ggcgcctccg ccaccgcgtc tgacccagag ggctcgacgg cccggcaggg
     1321 gcgcccgtcc ggccttggct gatggcgatc ggccctgata ggttgtccgt cccacgctcg
     1381 cccgcgcgcc tgccgctcct gcgccaggga gccgggcggg ggggcgcaac gccgccgcca
     1441 ccgtaccgac cgaacccgct gggagtgcga tgaccgtgga agcccggatc gtccggggcg
     1501 cggccctggc cacgaccctg gccgccccgg tcctggtcgc cgtggcctgg gtcgccgccg
     1561 ggcgcgccgg cgcggtcggg gcgctcctcg gcgtcatgct ggcggtcgcg ttcttcgccg
     1621 tcaccgtcgt ggtggtcggg atggcggcga aggtctccga cgacctcatg ctgcccgccg
     1681 ccctcggcac ctacctgctc aaggtggtcg gcctcggcgt ggtcttcctc accctgcacg
     1741 acaccaccgc gttcgaccgt tccgcgttcg cgatcgcgac cgtcgccggg acgtgcgtct
     1801 tcctggtcgc cgagctgcgg ctcgcggccc gcgcccgcat ccccgcggtc gtgaccgggg
     1861 accgggacgc ggtcgtgacc gggg
//
LOCUS       DASPYY010000712         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1802329, whole genome shotgun sequence.
ACCESSION   DASPYY010000712 DASPYY010000000
VERSION     DASPYY010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802329"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1933
                     /locus_tag="VEP73_11175"
     CDS             293..1933
                     /locus_tag="VEP73_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HYT27032.1"
                     /translation="MRVLGINAVFHDPAAALVVDGEIVAAAEEERFSRRKHGKPPVPF
                     ATWELPEQSASWCLAEAGLAPEDLDAVAYSYDPALAPQPGPDVTADDWEGLRTLYAQR
                     APLFLRTALPGLDPACVRYVPHHVAHAASAHLAAPFRSSSVLVLDGRGERASHLAGRS
                     VDGELEVLAAQELPHSLGLLYEEVTDHLGFRRSSDEYKVMALAAYGRPAHLRELREAV
                     RTTADGGFTVAPVELGTLAKRVRKGEPWTAEHADLAASVQLRLQEVLLELVRWLHART
                     GDRDLTMAGGVALNCVANSYLAERGPFERIWVQPAAGDAGTALGAALHVARALGDRVG
                     PMRGAALGRGWTDEELEAWLATAKVAHERPADLADAVAEVIAADGVVAWFQGRSEYGP
                     RALGHRSLLANAERAHNLERLNEVKGREQFRPVAPMVLAERAAEVFEGPLPSPYMLFT
                     QRVRPQWRERIPAVVHVDGSARIQTVDRAEEPLVASMLAAVERRTGVPVVVNTSLNTA
                     GRPMVDDPGDALECFGSAPVDVLALGPFLVRRTALARP"
     gene            1930..3039
                     /locus_tag="VEP73_11180"
     CDS             1930..3039
                     /locus_tag="VEP73_11180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT27033.1"
                     /translation="MTERPAPVPGDLGGGPVPGRARAGAPSAASAGSRPGADAPFDVV
                     VPTVGRASLARLLDALAGQAGELPGRVLLVDDRGDRRRALLPGGPPAGLAGRLDLLAG
                     RAAGPAAARNAGWRAATAPWVAFLDDDVVPGPGWAAALAGDLAGLPAAAAGSQGRLVV
                     PLPRHRRPTDWERNVHGLETARWATADLAYRRSVLAELGGFDERFPRAYREDADLGLR
                     VVRAGYRIERGRRSVVHPVRPAGPLVSVRLQAGNADDALMRALHGPGWRATAGVPRGR
                     RPRHLATTAAGLAALAALAAGRRRLAAAAAAGWLAGTAELALARVAPGPRTPAEVGTM
                     LATSVLLPPAATWHWLAGLAARRRLLADGARWAGR"
     gene            3036..>3484
                     /locus_tag="VEP73_11185"
     CDS             3036..>3484
                     /locus_tag="VEP73_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYT27034.1"
                     /translation="MTRPDAPTGREHVLVARQDSVGDVLLAGPAVRAVAAGAARVTLL
                     CGPRGRAAAELLPGVHEVVVEAAPWIDAEPEPVSRAATEAFVDGLARRGIDQAIVLTS
                     FHQSPLPLALLLRLAGVATVAATSVDYPGSLLDVRHRVDEDLHEVERA"
BASE COUNT          368 a         1398 c         1314 g          404 t
ORIGIN      
        1 cgacggcgac gggcggggcg gagggcggca tctgggctcc ccttgtcggc gcaccggacg
       61 ggacgccgga acgtagcacc gcggtgagcg gcgcaaacaa aagaaacctg agagccggtt
      121 aagagaatta tctcgaggtc gtttgcttct cgacccaacg gttattcagg agcctcccgt
      181 gcgggaccgc cagtgcaagc accgtgcagg tcgtgagcag cggaaaggac atgcagtgcg
      241 ccacccggct ttgggctgcc cgctcatccc cgccctcggg gggatctgag ccatgcgggt
      301 gctcggcatc aacgcggtgt tccatgaccc ggccgccgcc ctcgtcgtgg acggcgagat
      361 cgtcgccgcc gccgaggagg agcgcttcag ccggcgcaag cacggcaagc cgcccgtgcc
      421 gttcgcgacc tgggagctgc ccgagcagtc ggcctcctgg tgcctggccg aggccggtct
      481 ggccccggag gacctggacg cggtcgccta ctcctacgac cccgccctcg ccccccagcc
      541 ggggccggac gtgaccgccg acgactggga ggggctgcgg accctgtacg cgcagcgggc
      601 gccgctgttc ctgcgcaccg cgctgcccgg gctcgacccc gcgtgcgtcc gctacgtccc
      661 ccaccacgtc gcccacgccg cctccgccca cctggccgcc ccgttccggt cctcgagcgt
      721 gctcgtgctc gacgggcggg gcgagcgggc gtcccacctg gccggccgct cggtcgacgg
      781 ggaactcgag gtcctcgccg cccaggagct gccccactcg ctggggctgc tctacgagga
      841 ggtgaccgac cacctcggct tccggcgctc gtcggacgag tacaaggtga tggcgctcgc
      901 cgcctacggc cggcccgccc acctccggga gctgcgggaa gccgtgcgga ccaccgcgga
      961 cggcggcttc acggtcgccc cggtcgagct gggcaccctg gccaagcggg tccgcaaggg
     1021 cgagccgtgg accgcggagc acgccgacct cgcggcgagc gtccagctcc ggctgcagga
     1081 ggtgctgctc gagctggtgc gctggctgca cgcgcgcacc ggcgaccgcg acctcacgat
     1141 ggccggcggg gtggcgctca actgcgtcgc caactcctac ctggccgagc ggggcccctt
     1201 cgagcggatc tgggtgcagc ccgccgccgg cgacgccggg accgcgctcg gcgccgccct
     1261 ccacgtggcc agggcgctcg gcgaccgggt cgggcccatg cgcggcgcgg cgctcggccg
     1321 gggctggacc gacgaggagc tcgaggcctg gctcgccacc gccaaggtcg cccacgagcg
     1381 cccggccgac ctggccgacg ccgtcgccga ggtgatcgcg gccgacggcg tcgtcgcctg
     1441 gttccagggc cgcagcgagt acgggccgcg cgcgctcggg caccgcagcc tgctcgccaa
     1501 cgccgagcgg gcccacaacc tcgagcggct gaacgaggtg aaggggcggg agcagttccg
     1561 cccggtcgcg cccatggtgc tcgccgagcg ggccgccgag gtgttcgagg ggccgctgcc
     1621 gagcccctac atgctgttca cccagcgggt ccggccacag tggcgcgagc ggatcccggc
     1681 cgtcgtccac gtcgacggca gcgctcgcat ccagaccgtc gaccgcgccg aggagccgct
     1741 ggtggcgagc atgctcgcgg cggtggagcg ccgcaccggc gtccccgtgg tggtcaacac
     1801 cagcctgaac acggccgggc ggccgatggt ggacgacccg ggggacgcgc tcgagtgctt
     1861 cggctccgcg cccgtcgacg tgctcgccct cggcccgttc ctcgtccggc ggacggccct
     1921 cgcccgtcca tgaccgagcg ccccgcaccc gtccccgggg acctcggcgg cggcccggtc
     1981 cccggtcgcg cgcgcgccgg cgccccgtcc gccgcgtccg cgggctcgcg gccgggcgcc
     2041 gacgcaccct tcgacgtcgt cgtcccgacg gtggggcggg cgtcgctcgc gcgcctgctg
     2101 gacgcgctcg cgggccaggc cggcgagctg cccggccgtg tcctgctggt cgacgaccgc
     2161 ggggaccggc ggcgggcgct cctgcccggc gggccgcccg ccggcctcgc gggacggctc
     2221 gacctcctcg ccgggcgggc ggccgggccg gcggcggcca ggaacgccgg ctggcgggcc
     2281 gcgaccgcgc cctgggtcgc gttcctcgac gacgacgtcg tccccggccc gggctgggcg
     2341 gcggccctcg ccggcgacct cgccggcctc cccgccgcgg cggccggctc ccagggccgg
     2401 ctcgtggtac cgctgccacg ccaccggcgc ccgaccgact gggagcgcaa cgtccacggc
     2461 ctcgagacgg cgcgctgggc gaccgccgat ctcgcctacc ggcggtcggt cctcgccgag
     2521 ctgggcggct tcgacgagcg cttcccgcgc gcctaccgcg aggacgccga cctcggcctg
     2581 cgcgtggtcc gcgccgggta ccggatcgag cgcgggcggc gctcggtcgt ccaccccgtc
     2641 cggcccgccg gcccgctcgt cagcgtgcgc ctgcaggcgg gcaacgccga cgacgcgctc
     2701 atgcgagcgc tgcacggtcc gggctggcgt gccacggccg gggtgccgcg cggccgccgc
     2761 ccccgccacc tggccaccac cgccgccggg ctcgccgcgc tggccgcgct cgcggccggg
     2821 cgccgccgcc tggcggccgc cgccgcggcc ggctggcttg ccggcaccgc cgagctggcc
     2881 ttggcgcggg tcgcgcccgg cccccgcacc ccggccgagg tgggcaccat gctggccacg
     2941 agcgtgctgc tgccgcccgc cgcgacctgg cactggctgg ccggcctggc cgcgaggcgc
     3001 cgcctgctgg ccgacggcgc caggtgggcg ggccgatgac ccgcccggac gctccgaccg
     3061 ggcgggagca cgtgctcgtg gcccggcagg acagcgtcgg cgacgtgctg ctcgccgggc
     3121 ccgccgtgcg cgcggtcgcg gccggcgcgg cccgggtcac cctgctgtgc ggcccgcgcg
     3181 ggcgggcagc ggccgagctg ctgccaggcg tccacgaggt ggtggtcgaa gccgccccct
     3241 ggatcgacgc cgagcccgag ccggtcagcc gggccgccac cgaggcgttc gtcgacggcc
     3301 tcgcccggcg cggcatcgac caggcgatcg tgctcacgtc gttccaccag agcccgctgc
     3361 cgctggccct gctgctgcgg ctcgccgggg tggcgaccgt cgccgccacg agcgtcgact
     3421 accccggctc cctgctcgac gtccgccatc gcgtcgacga ggacctccac gaggtcgagc
     3481 gggc
//
LOCUS       DASPYY010000713         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2364033, whole genome shotgun sequence.
ACCESSION   DASPYY010000713 DASPYY010000000
VERSION     DASPYY010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364033"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VEP73_11190"
     CDS             <1..493
                     /locus_tag="VEP73_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HYT27035.1"
                     /translation="AGVLAVLAFVLIVVGSDIGRATGLSGAMSSLWAFLRWPLAIALV
                     VGAMALLFKRSPRRRQPAASWLAVGSAVSVLLWFVFTGLLSLYLTRSNTFGQTYGPLA
                     GLIGILLWTLLTALAVYLGIAFAAQLEAVRAGVPGPVTGEQRNTRGDGTRPTRRRTRP
                     VLP"
     gene            740..2476
                     /gene="treS"
                     /locus_tag="VEP73_11195"
     CDS             740..2476
                     /gene="treS"
                     /locus_tag="VEP73_11195"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HYT27036.1"
                     /translation="MRSGALGLEELRRMREDPLWYKTAVFYELYVRGFQDGNGDGQGD
                     FRGLTERLDYLQWLGIDCVWLLPMYPSPLRDGGYDVSDHFSILPDYGTLGDFYEFLEA
                     AHARGIRVIADMVMNHTSDQHPWFQEARRPGSLKRDWYVWSDTDQRYQDARVIFVDTE
                     ASNWTWDSQAQAFYWHRFFSHQPDLNYDNPEVQGAMLEVIRFWLDMGLDGFRLDAVPY
                     LFEREGTNCENLPETHEFLKRVRKEVDDKYPDRLLLAEANQWPPDVVPYFGDGDQCHM
                     AFNFPVMPRMFMALRREDRTPIVEIMNRTPKIPDSAQWGLFLRNHDELTLEMVTDEER
                     DYMYAEYAKDPRMRRNIGIARRLAPLLDNGRRQMELLYSLLLSLPGSPVIYYGDEIGM
                     GDNIYLGDRDGVRTPMQWTGDRNAGFSRADPAALYLPFLMDPVYGYQAQNVEAQERVS
                     TSLLQWIRRMLQVRKQHPVFGQGDYEPLYPDNRRVLAFVRQLGDDVVIVIANLSRFAQ
                     PVELDLGRWHGLVPEEMFGRTPFPMIDGGEPYRLAVGPHGFYWFHLVEPPDNPDDTAM
                     FRAVRGPLAGRH"
     gene            complement(2598..3248)
                     /gene="tenA"
                     /locus_tag="VEP73_11200"
     CDS             complement(2598..3248)
                     /gene="tenA"
                     /locus_tag="VEP73_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HYT27037.1"
                     /translation="MTIPASPSGRMFALARGGLDAQLAHPTVRGIADGTLGEAVFRRW
                     LVQDWLFLHDYVRVFALAAARAPDVESLGRLVDLAHATFHGELALHRSYAREFGLTDA
                     DLARARKSPACAAYTDFLLRTAATGDFADVLAALLPCMWGYSELGRAMAAAGMPADPR
                     YRRWVEAYADPGFAELAAWCARLLDRAAEGLPASRLEQLERCFGDSMDHELAFWDA"
     gene            3347..>4297
                     /locus_tag="VEP73_11205"
     CDS             3347..>4297
                     /locus_tag="VEP73_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27038.1"
                     /translation="MPDPYLADLVEAADPAALLRAVDGLCATRDWAGMAELRRRLVGA
                     VERGKPLWPLTTYVEYRTALEAPGPEAAGVLRPGAGRFALGPLTEVAGSSHGWEELAP
                     HLDDPGVAGAVAQERVLRGEDLRGDRRAHPEVLELPLALAGWEPSYALATYSADKLEV
                     PDPGAEPVAMAAASSEPGRVLERRGVVRALTDLVEVWTTESGGTARAVVVEGGPSGAV
                     AALGAGRHRLGRISGTGAVARMAWAAASGGAHGVRRGAALGRFDAWWAVAALAGVEWP
                     PGPEELGAAVERLSWWCWDDGISGSGWILRLAVADDGAGWS"
BASE COUNT          560 a         1565 c         1600 g          572 t
ORIGIN      
        1 ggccggcgtg ctggcggtgc tcgccttcgt gctcatcgtc gtcggctcgg acatcggccg
       61 agccaccggc ctgtccggcg cgatgagctc gctctgggcc ttcctccgct ggcccctggc
      121 catcgccctg gtggtcggcg cgatggcgct gctgttcaag cggtcgccgc ggcgcaggca
      181 gccggccgcc tcgtggctcg cggtgggatc ggcggtgtcg gtgctgctgt ggttcgtgtt
      241 cacggggctc ctgtcgctct acctgacccg gagcaacacc ttcgggcaga cctacgggcc
      301 gctcgccggc ctgatcggga tcctgctctg gacgctcctg accgcgctcg ccgtctacct
      361 cggcatcgcg ttcgcggccc agctcgaggc cgtccgggcc ggggtgcccg gcccggtcac
      421 cggcgagcag cgcaacacca ggggcgacgg cacccgcccc acccgccggc gcacccggcc
      481 ggtcctgccc tgaccgcccc tgagcgcccg ccggaccggc agaagtgcca ctgcagccgt
      541 ggcccgcgac ggccgatgag aaggagcggc ggcgcagttg gaacgatcct cgcgtgcggg
      601 gccgtccggc acgcgtggct tccgtcgacc accgcgatcc ggtcgcgaag gggttgagcg
      661 ccggtggcgt ggtggtgaag actggcctga tccagcgcag gcacgcatgg ggagccccgc
      721 gacgagggct tcaccggcta tgcggtcggg cgctctagga ctcgaggagc tacgacggat
      781 gcgggaagat cccctctggt acaagacggc cgtcttctat gagctgtacg tccggggctt
      841 ccaggacggc aacggcgacg gccagggcga cttccgcggg ctgaccgagc ggctggacta
      901 cctgcagtgg ctcggcatcg actgcgtctg gctgctcccg atgtacccct cgccgctgcg
      961 cgacggcggc tacgacgtga gcgaccactt ctcgatcctg cccgactacg gcaccctcgg
     1021 cgacttctac gagttcctgg aggccgccca cgcccgcggc atccgggtga tcgccgacat
     1081 ggtgatgaac cacacctccg accagcaccc ctggttccag gaggcgcgca ggcccggctc
     1141 gctgaagcgc gactggtacg tgtggagcga caccgaccag cgctaccagg acgcccgcgt
     1201 catcttcgtc gacaccgagg cgtcgaactg gacctgggac tcgcaggcgc aggcgttcta
     1261 ctggcaccgg ttcttctccc accagcccga cctcaactac gacaaccccg aggtccaggg
     1321 ggcgatgctc gaggtcatcc gcttctggct cgacatgggc ctcgacgggt tccggctgga
     1381 cgcggtgccc tacctgttcg agcgcgaggg gaccaactgc gagaacctcc ccgagaccca
     1441 cgagttcctg aagcgggtcc gcaaggaggt cgacgacaag taccccgacc ggctgctcct
     1501 ggccgaggcg aaccagtggc cgcccgacgt cgtcccctac ttcggcgacg gcgaccagtg
     1561 ccacatggcg ttcaacttcc cggtcatgcc acgcatgttc atggcgctgc ggcgggagga
     1621 ccggaccccg atcgtcgaga tcatgaaccg cacgccgaag atccccgact ccgcccagtg
     1681 ggggctgttc ctgcgcaacc acgacgagct caccctcgag atggtcaccg acgaggaacg
     1741 cgactacatg tacgcggagt acgccaagga cccgcgcatg cggcgcaaca tcggcatcgc
     1801 ccgccgcctc gcccccctgc tcgacaacgg ccgccgccag atggagctgc tctacagcct
     1861 gctgctgtcg ctgccgggca gcccggtcat ctactacggc gacgagatcg gcatgggcga
     1921 caacatctac ctcggcgacc gcgacggcgt gcgcacgccc atgcagtgga ccggcgaccg
     1981 caacgccggc ttctcgcgtg ccgacccggc agcgctctac ctgcccttcc tgatggaccc
     2041 ggtctacggc taccaggccc agaacgtcga ggcccaggag cgcgtgtcca cctcgctgct
     2101 gcagtggatc cggcggatgc tgcaggtccg caagcagcac ccggtgttcg gccagggcga
     2161 ctacgagccg ctgtaccccg acaaccgccg ggtgctcgcg ttcgtccgcc agctgggcga
     2221 cgacgtggtg atcgtgatcg ccaacctctc ccgcttcgcg cagccggtcg agctcgacct
     2281 cggccgctgg cacggcctcg tccccgagga gatgttcggg cgcaccccgt tcccgatgat
     2341 cgacggcggg gagccctacc ggctcgccgt cgggccccac ggcttctact ggttccacct
     2401 ggtcgagccc ccggacaacc ccgacgacac ggcgatgttc agggccgtgc ggggcccgct
     2461 ggcgggccgc cactgagcgc cggtcagccg tcggccggca ggcggtggtc ccgagccccg
     2521 gcgagcgcca ccctgccgcc gacctcggcc gtgccgccgg ggccgggccg cgcacgcagc
     2581 accgagccgc accggcctca cgcgtcccag aaggcgagct cgtggtccat gctgtcgccg
     2641 aagcaccgct cgagctgctc gagccgcgat gcgggcaggc cctcggcggc tcggtcgagc
     2701 agccgcgcgc accaggcggc cagctcggcg aacccggggt cggcgtaggc ctcgacccac
     2761 cgccggtagc gggggtcggc cggcatcccg gccgccgcca tcgccctgcc gagctccgag
     2821 tagccccaca tgcacggtag cagcgccgcc aggacgtcgg cgaagtcgcc ggtggcggcg
     2881 gtccggagca ggaagtcggt gtaggcggcg cacgccggcg acttccgggc gcgcgccagg
     2941 tcggcgtcgg tgaggccgaa ctcccgggcg taggagcggt gcagggccag ctcgccgtgg
     3001 aaggtggcgt gggcgaggtc gacgaggcgg ccgaggctct cgacgtccgg cgcccgggcg
     3061 gccgcgagcg cgaacacgcg cacgtagtcg tgcaggaaga gccagtcctg gacgagccag
     3121 cgccggaaca ccgcctcgcc gagcgtgccg tcggcgatcc cgcggaccgt cgggtgcgcg
     3181 agctgggcgt cgaggccgcc gcgagcgagc gcgaacatgc gcccgctcgg gcttgccggg
     3241 atggtcatcc accgatcgta gccggggccc gggaggccgc gcaggccccg ccggtggcaa
     3301 gatggccgac cgtacgccga gagcgcccgt ggctggagga gctgccgtgc ccgacccgta
     3361 cctcgcagac ctcgtggagg ccgccgaccc ggcggcgctg ctccgcgccg tggacggcct
     3421 ctgtgcgacc cgcgactggg cgggcatggc cgagctgcgc cggcggctgg tgggcgcggt
     3481 cgagcggggc aagccgctct ggccgctgac cacgtacgtc gagtaccgca cggcgctgga
     3541 ggcgccgggc ccggaggcgg ccggggtgct gcgccctggg gccggacggt tcgcgctcgg
     3601 cccgctcacc gaggtggccg ggtcgtcgca cgggtgggag gagctggccc cgcacctcga
     3661 cgacccgggc gtcgccggcg cggtcgccca ggagcgggtg ctgcgcggcg aggacctgcg
     3721 gggcgaccgg cgcgcgcacc ccgaggtgct cgagctgccg ctcgccctgg ccgggtggga
     3781 gccgtcgtac gcgctcgcga cctactccgc ggacaagctc gaggtgcccg acccgggtgc
     3841 cgagccggtc gcgatggcgg cagcctcgtc cgagccgggc cgcgtgctgg agcggcgcgg
     3901 ggtggtgcgg gccctgaccg acctcgtcga ggtgtggacg accgagtcgg gcgggaccgc
     3961 gcgggcggtg gtggtcgagg gcggcccgtc gggcgcggtg gccgccctcg gcgcgggccg
     4021 gcaccggctc ggccgcatct cggggaccgg cgcggtcgcg cggatggcgt gggcggccgc
     4081 gagcggcggg gcgcacgggg tgcgccgggg cgcggcactc gggcggttcg acgcctggtg
     4141 ggcggttgcc gcgctcgccg gcgtggaatg gccgccgggc ccggaggagc tcggtgccgc
     4201 ggtggagcgg ctgtcgtggt ggtgctggga cgacgggata tcgggcagcg gctggatcct
     4261 ccgcctggcg gtcgccgacg acggtgcggg ctggtcg
//
LOCUS       DASPYY010000714         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1522297, whole genome shotgun sequence.
ACCESSION   DASPYY010000714 DASPYY010000000
VERSION     DASPYY010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522297"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VEP73_11210"
     CDS             complement(<1..419)
                     /locus_tag="VEP73_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT27039.1"
                     /translation="MRTIDRTRPVAPARYEIDLGYYGGLDEQWWDPAGPAGLLHAMNR
                     PRLGFYLGQLPDPRGLQVLDAGCGGGLVAKGLAEAGAVVTGLDLSTGCLQVARRAVPH
                     GFRPAAGRMERLPFAAGSFDAAVAADVLEHVPDLPAVV"
     gene            complement(416..1588)
                     /locus_tag="VEP73_11215"
     CDS             complement(416..1588)
                     /locus_tag="VEP73_11215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT27040.1"
                     /translation="MPETDRPAAPRQDVVALAVRLAPALAARAAGYDEADAFAHQDVD
                     DLVASGYTTITVPADLGGLGASALDLVAAQSRLAEGSPATALAVNMHLHGVGILAEGM
                     RDRVEPFLKLVVTDGAMLAGGFSEPESGGNWWYQASTAVRVDGGYRLRGMKTFFTGFP
                     RATHLFLSAAVETAAGREPIGFLFPKPDKGVRVVGEWKAMGMRATGSHVLAIDDVLVP
                     DEHVVDRGFAVAASFLTGSHWSWPSFASVFLGAAEAAYRHVVDGLPRRRNEGLAQALS
                     ELPGVQQAVGEMRMHLDAARATLYAAVGRPPNPDPVAHYGEMAAAKLFVCQAAVHVCT
                     LALRTAGGSAYLRSGPVERLLRDAHAGLLLPPSHDATLQWVGRVELGAAGGPGSGP"
BASE COUNT          209 a          640 c          611 g          190 t
ORIGIN      
        1 accacggcgg gcaggtcggg gacgtgctcc aggacgtcgg cggccacggc cgcgtcgaag
       61 ctgccggcgg cgaacggcag ccgctccatc ctcccggcgg cgggccggaa gccgtggggc
      121 accgcgcgcc gggccacctg caggcagccc gtcgacaggt cgaggccggt cacgaccgcc
      181 cccgcctcgg ccaggccctt ggcgaccagg ccgccaccgc agcccgcgtc gagcacctgc
      241 aggccgcgcg ggtcggggag ctgtcccagg tagaacccga gccgcggccg gttcatcgcg
      301 tgcagcagcc cggcgggccc tgcggggtcc caccactgct cgtcgagccc gccgtagtac
      361 ccgaggtcga tctcgtaccg ggccggcgcc accggcctgg tccggtcgat cgtcctcacg
      421 ggccgctccc gggcccgccg gcggcgccga gctccacccg gccgacccac tgcagggtgg
      481 cgtcgtggga cggcggcagc agcaggccgg cgtgcgcgtc gcgcagcagc cgctccaccg
      541 gtccggagcg caggtaggcg ctgcccccgg cggtccgcag ggcgagcgtg cagacgtgca
      601 ccgccgcctg gcagacgaac agcttcgccg ccgccatctc gccgtagtgg gcgaccgggt
      661 cgggattcgg cggccgcccc accgccgcgt agagggtcgc ccgggccgcg tcgaggtgca
      721 tgcgcatctc cccgaccgcc tgctggaccc ctgggagctc cgagagggcc tgcgccaggc
      781 cctcgttccg ccgccgcggc aggccgtcga cgacgtgccg gtaggcagcc tcggcggcgc
      841 cgaggaagac gctcgcaaac gacggccacg accagtggga accggtcagg aagctcgccg
      901 ccaccgcgaa gccccggtcg acgacgtgct cgtcggggac gaggacgtcg tcgatcgcga
      961 gcacgtggct gccggtcgcc cgcatgccca tcgccttcca ctcccccacc acccggacgc
     1021 ccttgtccgg cttagggaac aggaagccga tcggctcacg gccggccgcg gtctcgaccg
     1081 cggccgagag gaacaggtgg gtcgcccgcg ggaacccggt gaagaacgtc ttcatcccgc
     1141 gcagccggta gccgccgtcg acccgcaccg cggtcgaggc ctggtaccac cagttcccgc
     1201 ccgactccgg ctcggagaac ccgcccgcga gcatcgcgcc gtcggtcacc accagcttga
     1261 ggaacggctc gaccctgtcg cgcatcccct cggcgaggat gccgacgccg tggaggtgca
     1321 tgttgaccgc cagggcggtg gccggggagc cctcggcgag ccggctctgg gcggcgacca
     1381 ggtcgagggc gctcgcgccg agcccgccca ggtcggcggg gaccgtgatg gtggtgtagc
     1441 cgctggcgac caggtcgtcg acgtcctggt gggcgaacgc gtcggcctcg tcgtagccgg
     1501 cggcccgcgc ggcgagggcc ggcgccagcc gcacggcgag cgcgacgacg tcctggcggg
     1561 gcgctgcggg acggtcggtt tcgggcatca ccctggctcc tgcgcacttc cggcggctgc
     1621 gggccgttcg atcttaccgg cctccccccc
//
LOCUS       DASPYY010000715         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1803206, whole genome shotgun sequence.
ACCESSION   DASPYY010000715 DASPYY010000000
VERSION     DASPYY010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803206"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VEP73_11220"
     CDS             <1..1297
                     /locus_tag="VEP73_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27041.1"
                     /translation="PAPGSPLSPVRPGGRPVADELTVEPDLRFDPAVPALPLVADLEA
                     VASLFAAQGHEVSELRRQDVKYTPLTRCVASYAVTITGGAGGRRRTVAAVVVTPAGTS
                     LRLADDDEGLPGLAEALDPACMLPRLDEALAPAERVTGCTATPVRYKFGARCTVRYDV
                     RTATGAGTLYGKLAADGWRERLATLAALARAGRDRPAMPGILPVVAHWGDLHLLLQPA
                     AEGAAELNQRAFDPAEPEKARDGWLWAAGRHLGGLHACRLPDALLSLAGGDLDELRAC
                     AAPMAQADQRLAHRYRSIVDLLEQRAGAGPPEPAVTSHGAMRTDQFLIVGDRLVMIDL
                     DTVRRSEPARDLGNLLAYLDWKAIRLPALAPFCERGARMVLDGYAGVRPAPEGPRLAL
                     QRATSLLKIAGRRYRSLTVREWPLVPRLLDAAATALASA"
     gene            complement(1358..>2356)
                     /locus_tag="VEP73_11225"
     CDS             complement(1358..>2356)
                     /locus_tag="VEP73_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27042.1"
                     /translation="KETPLYDPAKVFRAAAEGAMLPHGYERGSALAEAAAALDADSAA
                     WVELSRWVNSPHSELNERFVATLLLFGRLGASDGELAEAIVRFWSGDPIRVADLRRRL
                     RETGEERFALRAVPAHELARQRFRFAARLFAAAGYAGWVVLFDEVELIGRYSVLQRGR
                     SYAELARWVRGDETDPHLPLVAVVAMTDDFEAAVVSGKNDRELVPTKLRARQTHEADE
                     TAARAELGMRVVDREMLLLAPPDAAELDRAYARLKELHGEAFGWDPPEVAGLERLGAT
                     RMRQYVRAWINEWDLVRLDPEFAPATEVVGEAVETALGYREDAELEGAERDDPTAT"
BASE COUNT          278 a          937 c          849 g          292 t
ORIGIN      
        1 gcccgctcct ggctctccgt tgtcgccggt gcggccaggc ggccggcctg tcgccgacga
       61 gctgaccgtg gagcccgacc tgcgcttcga cccggcggtc cccgccctgc cgctggtcgc
      121 cgacctcgag gcggtggcct cgctgttcgc cgcccagggc cacgaggtga gcgagctgcg
      181 ccggcaggac gtcaagtaca cgccgctgac ccgctgcgtc gcctcctacg cggtcacgat
      241 caccggcggc gccggcgggc gccggcggac cgtcgccgcg gtcgtggtca cgccggcggg
      301 cacgagcctc cggctcgccg acgacgacga ggggctcccc ggtctcgccg aggcgctcga
      361 ccccgcgtgc atgctgcccc ggctcgacga ggcgctcgcg cccgccgagc gcgtgaccgg
      421 gtgcaccgcc acaccggtcc gctacaagtt cggcgcccgc tgcacggtcc gctacgacgt
      481 ccgcaccgcg accggtgcgg gcaccctcta cggcaagctc gccgccgacg ggtggcggga
      541 gcggctggcg acgctcgccg cgctcgcccg ggccgggaga gaccgtcccg cgatgccggg
      601 gatccttccg gtcgtcgcgc actggggcga cctgcacctg ctcctgcagc cggcggcgga
      661 gggggccgcc gagctcaatc agcgcgcctt cgacccggcc gagcccgaga aggcgcgcga
      721 tggctggctc tgggcggcag gtcgccacct gggcggcctg cacgcctgcc ggctaccgga
      781 cgccctgctg tcgctggcag gcggcgacct cgacgagctg cgcgcctgcg cggcgccgat
      841 ggcccaggcc gaccagcggc tcgcgcaccg ctaccggtcg atcgtcgacc tgctcgagca
      901 gcgcgcgggt gcggggccgc ccgagcccgc ggtcacgagc cacggggcca tgcggaccga
      961 ccagttcctg atcgtcggcg accgcctggt catgatcgac ctcgacaccg tgcgccgctc
     1021 cgagccggcc cgcgacctcg gcaacctgct cgcctacctt gactggaagg ccatccgcct
     1081 gccggcgctc gcccccttct gcgaacgcgg ggcgcgcatg gtcctcgacg ggtacgcggg
     1141 cgtccgcccc gcgcccgagg ggccgcggct ggccctgcag cgggcgacct cgctgctcaa
     1201 gatcgccggg cggcgctacc gcagcctcac cgtccgagag tggccgctcg tcccgaggct
     1261 cctggacgcc gcggccacgg cgctcgcaag cgcctgaccc tcgacgaccc ggtgcgcttc
     1321 cggctgcccg gcgtggaggc ggccgggccg aaccgggtca ggtcgcggtc gggtcgtcac
     1381 gctcggcgcc ctcgagctcg gcgtcctccc ggtagccgag cgcggtctcg accgcctcgc
     1441 ccaccacctc ggtggcgggc gcgaactccg ggtcgagccg gaccaggtcc cactcgttga
     1501 tccaggcccg cacgtactgg cgcatccggg tggcaccgag gcgctccagt cccgcgacct
     1561 cgggcgggtc ccagccgaag gcctcgccgt gcagctcctt caggcgcgcg taggcgcgat
     1621 ccagctccgc cgcgtcgggc ggtgcgagca ggagcatctc ccggtcgacg acccgcatgc
     1681 cgagctcggc gcgagcggcg gtctcgtcgg cctcgtgggt ctggcgtgcc cggagcttcg
     1741 tcggcaccag ctcacggtcg ttcttgcccg acaccaccgc cgcctcgaag tcgtcggtca
     1801 tggccaccac ggcgaccagc gggaggtgcg ggtcggtctc gtcgccccgc acccagcggg
     1861 ccagctcggc gtaggagcgg ccccgctgga gcaccgagta gcggccgatc agctcgacct
     1921 cgtcgaacag gaccacccag ccggcatacc cggccgcggc gaacaggcgg gcggcgaagc
     1981 ggaaccgctg tcgcgcgagc tcgtgcgcgg ggacggcccg cagggcgaag cgctcctccc
     2041 cggtctctcg gagccggcgg cgcaggtcgg ccacgcggat cgggtcgccg gaccagaatc
     2101 gtacgatcgc ctcggccagc tccccgtcgc tcgcgccgag ccggccgaac aacagcagcg
     2161 tcgcgacgaa ccgctcgttg agctcgctgt ggggagagtt cacccagcgg gacagctcga
     2221 cccaggcggc gctgtcggcg tcgagcgcgg ccgccgcctc ggccagcgcc gagccccgct
     2281 cgtacccgtg cggcaacatg gcgccctcgg ccgccgcacg gaacaccttg gccgggtcgt
     2341 acagcggcgt ctcctt
//
LOCUS       DASPYY010000716         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1662382, whole genome shotgun sequence.
ACCESSION   DASPYY010000716 DASPYY010000000
VERSION     DASPYY010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662382"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VEP73_11230"
     CDS             complement(<1..666)
                     /locus_tag="VEP73_11230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYT27043.1"
                     /translation="MLHADLDAFYASVEVLKDPSLAGRPLLVGGVGPRGVVTSASYEA
                     RAFGCRNAMPMARARAMCPQAVAVPPDFAAYRRCSSRVMRVFRDLTPLVEPLSLDEAF
                     LDVAGARRLFGDAVRIARLLRARVAAETGLPLTVGVAGNKFLAKLASTRGKPDGLLVV
                     PPSRALDFLHPLPAEALWGAGRATIERLARYGLRTVGEVAATPRATLERVLGTAAGGQ
                     LSEL"
     gene            658..1278
                     /locus_tag="VEP73_11235"
     CDS             658..1278
                     /locus_tag="VEP73_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HYT27044.1"
                     /translation="MEHPEPVEVPLRDGGTADDDLAGPGRRHRAGPARHVFPVRGCVV
                     SYGRTHHDYPATDIFARRGCAFVSPVDGVVDEVSPVDRWDPGTNRGADRGGLSVSVVG
                     VDGVRYYGSHLGSVAPGIRPGVRVAAGTLLGRVSNTGSARGIATHLHFGLSWPTRHGI
                     WWVRRGVVYPWPFLDSWRAGGNLSPARAVRAARARTGTIPPCAAEC"
     gene            complement(1279..>2406)
                     /locus_tag="VEP73_11240"
     CDS             complement(1279..>2406)
                     /locus_tag="VEP73_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27045.1"
                     /translation="DGLGELVLVDVAGFGALARVRAGVASGRREWWPAGALVGRAIAA
                     YHEQFGQPVALLAPHASQDLLTQAAVLDAGLDQRVEVGTPHRFQGRELPVAVFDLVED
                     WRERGWLARGRIGGTRHELRALRLFDVGATRATGRTYLLADRLAVERATGGPLARIRE
                     MVGEGRISVAAAGRFVPLRRCPGTGEAVAAVPPALPAYEPAAFFGRLERDLAAARGEV
                     WVWSPFLATRPRPVLPWLVDAAERGCRVTVFVKPGHEHRPNDRRRVGALRDAGIAVVG
                     VYGLAERLVVVDGRLSFLGNLSVLGTPRERGEAMVGVEGGRFAARLLDLTQAEAFSAP
                     PACSDHPGERCHAQHYRRGREKGWFWTCPRCDRRTPVVLGT"
BASE COUNT          290 a          955 c          914 g          247 t
ORIGIN      
        1 gagctcggag agctggcccc cggcggccgt gccgagcacc cgctcgaggg tcgcccgagg
       61 cgtcgccgcc acctccccga cggtgcgcag gccgtagcgg gcgagccgct cgatggtggc
      121 ccgcccggcg ccccacaggg cctcggccgg cagcgggtgg aggaagtcga gggcgcggct
      181 cggcgggacg accagcaggc cgtccggctt gcccctggtc gaggccagct tggccaggaa
      241 cttgttgccg gccacgccga cggtgagggg caggccggtc tcggcggcga cccgggcccg
      301 gagcaggcgg gcgatgcgga cggcgtcgcc gaacaggcgg cgggcaccgg ccacgtcgag
      361 gaacgcctcg tcgagcgaga gcggctcgac cagcggggtg aggtcccgga agacccgcat
      421 cacccggctc gagcagcgcc ggtaggcggc gaagtcgggc ggcacggcga ccgcctgcgg
      481 gcacatggcc cgggcgcgcg ccatcggcat cgcgttgcgg catccgaacg ccctggcctc
      541 gtaggaggcg gaggtgacca cgccccgcgg cccgacgccg ccgacgagca gcgggcgccc
      601 ggcgaggctc gggtccttga gcacctccac gctcgcgtag aaggcgtcga ggtcggcgtg
      661 gagcacccgg agcccgtcga ggtccccctc cgggacgggg ggaccgccga cgacgacctc
      721 gccggtcccg gccggcggca ccgggccggg ccggccaggc acgtcttccc ggtgcggggg
      781 tgcgtggtct cctacggccg cacccaccac gactacccgg ccaccgacat cttcgcccgc
      841 cgcggctgcg ccttcgtctc cccggtcgac ggggtcgtcg acgaggtgag cccggtcgac
      901 cgctgggacc ccggcaccaa ccggggcgcc gaccggggcg gcctgtccgt ctcggtggtc
      961 ggcgtcgacg gcgtccgcta ctacggctcg cacctcggct cggtcgcgcc cggcatccgg
     1021 ccgggcgtgc gcgtggcggc cgggactctg ctcggccgcg tctccaacac cggctcggcc
     1081 cgcgggatcg ccacccacct gcacttcggg ctctcgtggc cgacgcggca cggcatctgg
     1141 tgggtccgca ggggggtcgt ctacccctgg ccgttcctcg actcctggcg cgcgggcggc
     1201 aacctctccc ccgcccgggc cgtgcgcgcc gcccgggcga ggaccggcac gatcccgccc
     1261 tgcgccgccg agtgctgatc aggtcccgag cacgaccggc gtccgccggt cgcacctggg
     1321 acaggtccag aaccagccct tctcccgccc gcgccggtag tgctgggcgt ggcagcgctc
     1381 gccgggatgg tcggagcagg cggggggcgc gctgaacgcc tcggcctgcg tgaggtcgag
     1441 aagccgcgcc gcgaagcggc cgccctcgac gcccaccatc gcctcgcccc gctccctcgg
     1501 cgtgccgagc acgctcaggt tcccgaggaa cgacaggcgg ccgtcgacca ccaccaggcg
     1561 ctcggccagg ccgtagacgc ccaccacggc gatgccggcg tcgcgcagcg cgcccacccg
     1621 ccgccggtcg ttcggccggt gctcgtggcc gggcttgacg aacacggtga cccggcagcc
     1681 gcgctcggcg gcgtccacga gccacgggag caccgggcgc ggcctggtgg ccaggaacgg
     1741 gctccagacc cacacctccc cgcgggccgc ggccaggtcg cgctccagcc ggccgaagaa
     1801 cgcggccggc tcgtaggccg gcagcgcggg cgggaccgcc gcgacggctt cgccggtccc
     1861 cgggcagcgg cgcagcggga cgaagcggcc ggcggcggcg accgagatcc tgccctcgcc
     1921 gaccatctcc cggatgcggg cgagcggccc gcccgtggcc cgctcgaccg cgagccggtc
     1981 ggcgagcagg tacgtgcgcc cggtcgcccg ggtcgcgccg acgtcgaaca ggcggagggc
     2041 ccgcagctcg tggcgggtgc cgccgatgcg gcccctggcc agccagccgc gctcgcgcca
     2101 gtcctcgacc aggtcgaaca cggcgacggg gagctcgcgg ccctggaagc gatgcggcgt
     2161 gccgacctcc acgcgctggt ccagaccggc gtcgagcacc gccgcctgcg tgagcaggtc
     2221 ctgcgacgcg tgcggcgcga gcagcgcgac gggctgcccg aactgctcgt ggtacgcggc
     2281 gatcgcccgg ccgaccaggg cgccggccgg ccaccactcg cgccggcccg acgcgacgcc
     2341 cgccctgacc ctcgccaggg cgccgaagcc cgccacgtcg acgagcacca gctcgccgag
     2401 cccgtc
//
LOCUS       DASPYY010000717         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1803654, whole genome shotgun sequence.
ACCESSION   DASPYY010000717 DASPYY010000000
VERSION     DASPYY010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803654"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VEP73_11245"
     CDS             <1..284
                     /locus_tag="VEP73_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT27046.1"
                     /translation="DAAAALYAPDAEYYEPGNELHRGRDEIRNFLAAYFANRGPVEVT
                     VKREVTADSTVLAEWTSAYTQAGRRSSGTPGVSVVEVSSHGIRYHRDYQ"
     gene            complement(292..528)
                     /locus_tag="VEP73_11250"
     CDS             complement(292..528)
                     /locus_tag="VEP73_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HYT27047.1"
                     /translation="MSTEVKLGVTYSPRELVVETEEPAERIAGLVDEAVGGKTGVLWL
                     SDVNGRRVGIPTEKLAYVEVGEENQAKRVGFSSV"
     gene            762..1520
                     /locus_tag="VEP73_11255"
     CDS             762..1520
                     /locus_tag="VEP73_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27048.1"
                     /translation="MLLIGPFHCRADDVREFLWALAGGGLPLTRRGRLHLADLRRLHE
                     RLPGGGRLGRSRSAVALARRRAALLHSLCEAGRLVEVDRAALRLTSHGWEFLALPPGA
                     QTGFVFAAWWEGVDWGRWSPRAGLVRLLRRERDALLRELAALPEGRVDLASFARRFRA
                     LVGHCWPAGLAAAGPEVWRRELWGTALAPLAMLGALDVPGEVTAEPPRWFSVNDSSGE
                     LLGAAAAISGRGAEHAATPPGRPAARPRTDPGRN"
BASE COUNT          186 a          684 c          652 g          236 t
ORIGIN      
        1 cggacgccgc ggccgcgctg tacgcgccgg acgcggagta ctacgagccc ggcaacgagc
       61 tccaccgcgg ccgggacgag atccgcaact tcctggccgc ctacttcgcc aaccggggcc
      121 cggtcgaggt gacggtcaag cgcgaggtca ccgccgactc gaccgtcctg gccgagtgga
      181 cgagcgccta cacgcaggcg ggcaggcgct cgtccggtac ccccggggtg tcggtcgtcg
      241 aggtctcgag ccacggcatc cgctaccacc gcgactacca gtgacgcggc gctacaccga
      301 gctgaagccg acccgcttcg cctggttctc ctcgccgacc tcgacgtagg cgagcttctc
      361 ggtggggatg cccacccggc ggccgttgac gtcgctcagc cagagcacgc cggtcttgcc
      421 ccccaccgcc tcgtcgacca gcccggcgat gcgctcagct ggctcctccg tctcgaccac
      481 gagctccctg gggctgtagg tgacgccgag cttgacctcg gttgacatgg ctctcctcct
      541 tgacggccgg tgcgtggacg gccggtgcgc gcgggttcgg cgcggctcgc ccctgggcgg
      601 cgcgcggtcg ggggcgagcg taccaacggg gcggcaccgg cgcgtggccc ggctggcgcc
      661 cgcgggacgg gaggggctgt accggcctca ccgaaccaca taggctttcc aggggggccc
      721 tcggcccggc cgtgtccatt cctccacccg aggatcgcca cgtgctactg atcggaccat
      781 tccactgccg cgccgacgac gtccgcgagt tcctctgggc gctcgccggc ggcggcctgc
      841 cgctgacccg gcgcggcagg cttcacctgg ccgacctgcg ccggctgcac gagcggctcc
      901 cgggcggcgg gcggctcggc cgcagccgct ccgcggtcgc gctcgccagg cgccgggccg
      961 cgctcctgca ctcgctgtgc gaggcgggcc ggctggtcga ggtggaccgg gcggcgctcc
     1021 ggctcacgag ccacggatgg gagttcctcg ccctgccccc gggcgcgcag acgggcttcg
     1081 tgttcgcggc ctggtgggag ggggtggact ggggccgctg gtcgccgcgg gccgggctcg
     1141 tccgtctgct ccgccgggag cgggacgccc tgctgcgcga gctcgccgcg ctccccgagg
     1201 ggcgcgtcga cctggccagc ttcgccaggc gcttccgggc gctggtgggc cactgctggc
     1261 cggccggcct cgccgccgcc ggcccggagg tctggcggcg cgagctgtgg gggaccgccc
     1321 tggccccgct cgccatgctc ggcgcgctgg acgtccccgg cgaggtcacg gccgagccgc
     1381 cgcgctggtt ctcggtcaac gactcgagcg gcgagctgct cggtgccgcg gcggccatct
     1441 cgggtcgggg cgccgagcac gccgccacgc cacccggccg cccggccgcc cggccgcgaa
     1501 ctgaccctgg ccgcaactga ccggcctgtg ccccggcctg cctgcctcgc acggcgagcg
     1561 caggagcgcg gcggcgggaa ggtcggtcgc cccccccacc gccgcgcccg ccgtgccgcc
     1621 tccgtcaggc ggcgatcgcc tcgacgacga gggtccagtc gtccaggcgc aggatggtcg
     1681 ccgactgggt cgtcttgtac tgcaccctca ccgtgtaggt gcccggggtg aagtaggccc
     1741 gcgagcgctc cacggcat
//
LOCUS       DASPYY010000718         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_819951, whole genome shotgun sequence.
ACCESSION   DASPYY010000718 DASPYY010000000
VERSION     DASPYY010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819951"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..831
                     /locus_tag="VEP73_11260"
     CDS             163..831
                     /locus_tag="VEP73_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT27049.1"
                     /translation="MGIRVLVVDDQGLVRAGFRMILEAQPDIEVVGEAADGEEAVAAA
                     RRLAPDVVLMDIRMPRMDGLEATRRLAGPGVDDPVRVLVLTTFDLDDYVFEALRAGAS
                     GFLLKDLPREELVSAVRVVAGGDALLAPSITRRLIEEFARQPARAEYVPAALDSLTAR
                     ERETLELMARGMSNAEMAAALYVSEHTVKTHVGNVLAKLGLRDRIHAVILAYEVGLVH
                     PGRT"
     gene            complement(976..1788)
                     /locus_tag="VEP73_11265"
     CDS             complement(976..1788)
                     /locus_tag="VEP73_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYT27050.1"
                     /translation="MTDRYADAFPSLVFERPAEGVLRIVMSAPGRLNAAGPEMHRDLA
                     AVWTEVDRDPEVRAVVVRGAGDVFSAGGDLDLVADMADDFAVRTRVLREARDLVYNVI
                     NCSKPIVSAMQGPAVGAGLVVGLLADVSIAARSARIIDGHTRLGVAAGDHAAIVWPLL
                     CGMAKSKYHLLLCEPLSGEEAERIGLVSLCVDDARLHDTALDVAGRLSRGSQSAIRWT
                     KYALNNWLRMAGPTFDASLALEFMGFAGPDVREGIASLREQRRPDFQGPAAG"
     gene            complement(1867..2442)
                     /locus_tag="VEP73_11270"
     CDS             complement(1867..2442)
                     /locus_tag="VEP73_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HYT27051.1"
                     /translation="MGDEDGAGRRPPAPGPLVVVQAFVNSRDLAAGTDELATAAGLPD
                     WLARHGLLGDGEPATGADLRRAVELREALRVLLLANNGGLAEPAALALVNRALRDAEP
                     LVRFLPGGQVEMGALAPGVTGALGHLLCIAFTAMTDGTWARLKACREDGCRRAFYDQS
                     RNRSGTWCAMATCGSRAKMRAYYRRRHPGAP"
     gene            2621..>3192
                     /locus_tag="VEP73_11275"
     CDS             2621..>3192
                     /locus_tag="VEP73_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27052.1"
                     /translation="MPRVLTAAALFLAVLLAACTGSTRAGGPAPGAAPSATTTAASGS
                     SASGAGAGPSTTGATTTARTWSSAPKVRTRQVAPAPTLVDVRLAHHDTYDRIVFEFRG
                     DAPGYRVEYVPEVHEDATGDPVHLRGNAFLNVVLTPASMHDPQGASTYPGRKVLTAML
                     PTLRQARFAGDFEGQVSWGLGLDDTVGFKVG"
BASE COUNT          413 a         1182 c         1197 g          400 t
ORIGIN      
        1 ctgcaggccg gccccgggct cgagggcggc ttcgcggtcg tcgcccgcct ccccgtcgag
       61 ccggccgcgc gccttcccat cgagccggcc gggtaacgtc tgggcggcac gggggccggg
      121 taccatctgg gcggcacggg accgacgggg aagggcgggc cggtgggcat ccgggtgctg
      181 gtggtggacg accaggggct ggtcagggcc ggcttccgca tgatcctcga ggcccagccc
      241 gacatcgagg tggtcggcga ggcggccgac ggcgaggagg cggtcgccgc ggcgcggcgc
      301 ctcgcgcccg acgtggtcct gatggacatc cgcatgccgc ggatggacgg gctggaggcg
      361 accaggcggc tcgccggccc cggggtcgac gacccggttc gggtgctggt gctcaccacc
      421 ttcgacctcg acgactacgt gttcgaggcg ctacgggcgg gcgcgagcgg cttcctgctc
      481 aaggacctcc cgcgcgagga gctggtcagc gcggtgcggg tggtggcggg cggggacgcc
      541 ctgctcgcgc cctcgatcac caggcgcctg atcgaggagt tcgcccgcca gccagcccgt
      601 gccgagtacg tgcccgccgc gctcgactcg ctcaccgccc gcgagcggga gaccctcgag
      661 ctgatggccc gcggcatgtc caacgccgag atggccgccg ccctctacgt gagcgagcac
      721 accgtcaaga cccacgtcgg caacgtgctc gccaagctcg gcctgcgcga ccgcatccac
      781 gcggtgatcc tggcctacga ggtcggcctg gtgcaccccg gccggaccta gcccggccgg
      841 gcccccggag gcgccctcat atcatcaatc ggcgcgctcc gcgcccgcca ggtcaccggc
      901 aaggtcgtgc ccacccgctc ctgaccgccc gatcggtcct ggccagcgtg ccacagcgga
      961 cgggttcgcg gccggctacc cggcggcagg gccctggaag tcgggccggc gctgctcgcg
     1021 gagcgaggcg atcccctcgc ggacgtcggg gcccgcgaaa cccatgaact cgagcgcgag
     1081 cgaggcgtcg aacgtcgggc cggccatgcg cagccagttg ttcagggcgt acttcgtcca
     1141 gcggatcgcg ctctgggagc ctcgggagag cctgccggcc acgtcgagcg cggtgtcgtg
     1201 cagccgcgcg tcgtcgacgc agagcgacac gagcccgatg cgctcggcct cctcgccgga
     1261 cagcggctcg cacagcagca ggtggtactt gctcttcgcc atgccgcaga gcagcggcca
     1321 cacgatcgcg gcgtggtcgc ccgcggccac gccgaggcgg gtatggccgt cgatgatgcg
     1381 ggccgaccgg gcggcgatcg agacgtccgc gagcagcccg acgaccagcc cggcgccgac
     1441 cgccgggccc tgcatggccg agacgatcgg cttggagcag ttgatgacgt tgtagacgag
     1501 gtcgcgcgcc tccctgagca cgcgggtgcg gacggcgaag tcgtcggcca tgtcggccac
     1561 gaggtcgagg tcgccgccgg ccgagaagac gtcgcccgcg ccccggacga ccaccgcccg
     1621 cacctcgggg tcgcggtcga cctcggtcca gaccgcggcc aggtcccggt gcatctcggg
     1681 gccggccgcg ttcagcctgc ccggcgcgct catcacgatc cgcagcacgc cctcggcggg
     1741 ccgctcgaac acgaggctcg ggaaggcgtc ggcgtaccgg tcggtcatct cgtcctcgtt
     1801 cctcggctcg gcgggcgctg cgccgcccgg ctggggacag cctatgccgc cgctgacgcc
     1861 gacggcctac ggtgcgccgg ggtgccggcg ccggtagtag gcccgcatct tcgcgcggct
     1921 gccgcacgtc gccatcgcgc accacgtccc cgagcggttc ctggactggt cgtagaaggc
     1981 ccgccggcag ccgtcctcgc ggcaggcctt gagccgcgcc caggtgccgt cggtcatggc
     2041 cgtgaaggcg atgcagagca ggtggccgag cgcgccggtg acgcccgggg ccagcgcgcc
     2101 catctcgacc tggccgccgg gcaggaagcg gacgagcggc tcggcgtcgc gcagggcccg
     2161 gttgacgagc gcgagcgccg ccggttcggc caggcccccg ttgttggcca ggagcaggac
     2221 gcgcagcgcc tcgcggagct cgacggcccg ccggaggtcc gctccggtgg cgggctcgcc
     2281 gtcacccagc aggccgtggc gggcgagcca gtccggcagc ccggcagcgg tggccagctc
     2341 gtcggtgccc gcggcgaggt cccgggagtt cacgaacgcc tgcaccacca cgagcggccc
     2401 cggtgccggc ggacgccggc cggcgccgtc ctcgtccccc atgacttccg cctcctctct
     2461 tactagtcat catattgact ggtaagtctt accagcgtag cgtccgcggc ggcaagccgg
     2521 cgagccgggg gcaggaggtc gcgatgaccg cggcggcgaa cacgcggatg ctgcacgatg
     2581 gcgccatcgg gtcgccgtcg ccgacgcaag gggaatgtcc atgccacgag tcctgaccgc
     2641 cgccgcgctg ttcctcgcgg tgctcctggc cgcctgcacg ggctcgaccc gggcgggagg
     2701 gcccgcgccg ggcgccgccc cgtcggccac gaccaccgcg gcgtccgggt cgtcggcgtc
     2761 gggggctggg gcagggccct cgacgaccgg cgcgacgacc acggcgagga cgtggtcgag
     2821 cgccccgaag gtcaggaccc gccaggtggc tccggcgccg acgctggtgg acgtgcggct
     2881 cgcccaccac gacacgtacg accggatcgt gttcgagttc cgcggcgacg cacccggcta
     2941 ccgcgtcgag tacgtccccg aggtccacga ggacgccacc ggcgacccgg tccacctgcg
     3001 gggcaacgcc ttcctgaacg tcgtgctcac ccccgcgagc atgcacgacc cgcagggcgc
     3061 ctcgacctac cctggaagga aggtcctcac ggcgatgctg ccgacgctgc ggcaggcgag
     3121 gttcgcgggc gacttcgagg ggcaggtgag ctgggggctc gggctggacg acacggtcgg
     3181 gttcaaggtc gg
//
LOCUS       DASPYY010000719         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_396663, whole genome shotgun sequence.
ACCESSION   DASPYY010000719 DASPYY010000000
VERSION     DASPYY010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396663"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VEP73_11280"
     CDS             complement(<1..1864)
                     /locus_tag="VEP73_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYT27053.1"
                     /translation="MTELADGQAVTRRADRSGLSLAEAQALGAELRRAVRGEVRFDRG
                     SRALYATDGSNYRQVPIGVVCPLDDQDVIATVAACRRFGAPVLGRGGGTSLAGQGCNV
                     AVVVDMSKYMHDVVWLDPGRRLAQVQPGIVLDRLREAAEEHHLTFGPDPATHTHCTLG
                     GMIGNNSCGVHALMNGKTVDNVEALDVLTYDGLRMTVGPTGEEELASIVAAGGRRGRI
                     YAGLRDLRDRYADLVRRRYPRIPRRVSGYNLDELLPERGFNLAAALAGTEGTCVTVLE
                     ATLKLIPRPASRSLLVAGYDDIFSGGTHLAEILEARPVGCETFDHALFDNEHVQRRHG
                     RVLSQLPQGRAWVLIELGGETRGDSDDAARRLAARLGGHADPPRGLALFDDPDQQRAL
                     WEVREAGLGASSFPPGADDRWAGWEDSAVAPEDIGAYLRDLQGLHDRYGYRGAMYGHL
                     GDGCVHTRTSFDLRTPTGVATYRAFLEEAADLVVSYGGSLSGEHGDGHQRAELLPRMF
                     GEELVQAFRAFKATWDPEGKMNPGKVVDPYRLDQHLKLWPSSLPSRPSVSFSYPEDGG
                     DFAHATLRCVGVGTCRRPQPAEVVCPSFMVTREERHTTRGRSRLLFEMLEGDVVT"
BASE COUNT          312 a          849 c          857 g          308 t
ORIGIN      
        1 cggtgacgac gtcgccctcg agcatctcga agagcagccg tgaccggccc cgcgtggtat
       61 gccgctcctc cctggtgacc atgaagctcg ggcacaccac ctcggccggc tgcggcctcc
      121 ggcacgtgcc gaccccgacg cagcgcagcg tggcatgggc gaagtcgccc ccgtcctccg
      181 ggtaggagaa gctcaccgac ggccgcgacg ggagcgagga gggccagagc ttcaggtgct
      241 ggtccaggcg gtacgggtcc acgaccttgc ccgggttcat cttcccttcc gggtcccagg
      301 tcgccttgaa ggcgcggaag gcctgaacca gctcctcgcc gaacatccgg ggcagcagct
      361 ccgcgcgctg gtgcccgtcg ccgtgctctc ccgagagcga tcccccgtac gacaccacca
      421 ggtcggcagc ctcctcgagg aaggcacggt aggtggcgac gccggtgggt gtgcgcaggt
      481 cgaacgaggt ccgggtgtgc acgcacccgt cgccgaggtg gccgtacatg gccccccggt
      541 acccgtaccg gtcgtggagg ccctgcaggt cacgcaggta ggcgccgatg tcttccggcg
      601 caaccgccga gtcctcccag ccggcccacc ggtcgtccgc gccgggaggg aatgacgatg
      661 ccccgaggcc cgcctcccgc acctcccaca gcgcccgctg ttggtcggga tcgtcgaaca
      721 gcgccaggcc ccgcggggga tccgcgtggc cgccgaggcg cgccgccagc cggcgcgcgg
      781 cgtcgtcgga atcccccctg gtctccccgc cgagctcgat gagcacccag gcgcgtccct
      841 gcggaagctg cgacagcacc cgcccgtggc gccgctgcac gtgctcgttg tcgaagaggg
      901 cgtggtcgaa ggtctcgcac ccgacgggcc gcgcctccag gatctcggcg aggtgggtgc
      961 caccgctgaa gatgtcgtcg taaccggcaa ccagcaggct gcggctggcg ggacgaggga
     1021 tcagcttgag ggtggcctcg agcaccgtga cgcaggtacc ctcggtgccg gcgagcgcgg
     1081 cggcgaggtt gaagccgcgc tcgggcagga gctcgtcgag gttgtagccg gagacccggc
     1141 gggggatgcg cgggtaccgc cggcggacga ggtcggcgta ccggtcccgc aggtcgcgca
     1201 ggccggcgta gatccgcccc cggcgcccgc cggccgcgac gatcgacgcg agctcctcct
     1261 cgccggtggg cccgaccgtc atccgcagcc cgtcgtaggt cagcacgtcg agcgcctcga
     1321 cgttgtcgac cgtcttgccg ttcatcaggg cgtgcacgcc gcaggagttg ttgccgatca
     1381 tcccgccgag cgtgcagtgg gtgtgggtgg ccgggtcagg gccgaacgtg aggtggtgct
     1441 cctcggccgc ctcgcgcagg cggtcgagca cgatccccgg ctgcacctgg gcgagccggc
     1501 ggcctgggtc cagccagacg acgtcgtgca tgtacttcga catgtcgacc accacggcca
     1561 cgttgcagcc ctgcccggcg aggctggtgc ccccgccccg gccgagcacc ggcgccccga
     1621 accggcggca ggcggccacc gtcgcgatca cgtcctggtc gtcgagcggg cagaccaccc
     1681 cgatggggac ctggcggtag ttcgacccgt cggtggcgta cagcgcccgg ctgccccggt
     1741 cgaagcggac ctcgccccgg accgcgcgcc gcaactcggc accgagcgcc tgcgcctcgg
     1801 caagcgagag gccgctgcgg tcggcccgcc tggtgacggc ctggccgtcg gcgagctcgg
     1861 tcatgacgct cctcccctgc atgcttcctg ctggcgcgcc tggagcaccg ccggtggcgc
     1921 acccggagca ttgccaggcc gagccgagga taacgccgcc accgcgcctg ctccggcggc
     1981 atccggcggg catgcgacgc cgctaccacc tcgcgcgtga gcctccggat gctcgacgtt
     2041 cgcaagctcg ccagtgtgcg cttcgcgatg accggtgggg gggcgatgtg ccgcgcactg
     2101 cccttcccgg acgggcggcc tgggggcgca gggcccggcg accggggcgt tcgagcagcg
     2161 gaaggctttt agcatcccgg cgccgactga gacaagccgg cgcgccgcgc gggccatgag
     2221 cgggtcatga cgtgtagtag tcggccaggc cgtcgaggag cccgccgagg atcggcgcgg
     2281 cccggacggc ggcgaagaag gcggcgtcgt ggaccggcgg gtcgat
//
LOCUS       DASPYY010000720         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2787424, whole genome shotgun sequence.
ACCESSION   DASPYY010000720 DASPYY010000000
VERSION     DASPYY010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787424"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEP73_11285"
     CDS             complement(<1..184)
                     /locus_tag="VEP73_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27054.1"
                     /translation="MDTNPPFPPVSPAVRVTVLAKLAGEGFEDAFGTTADWLAVPAPD
                     LAELREAVGAERVSQLS"
     gene            complement(244..675)
                     /gene="rnpB"
                     /locus_tag="VEP73_11290"
     ncRNA           complement(244..675)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEP73_11290"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            788..1330
                     /locus_tag="VEP73_11295"
     CDS             788..1330
                     /locus_tag="VEP73_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HYT27055.1"
                     /translation="MLPALVLAFIVVPIAELAVILAIGHSIGFLPTLLLVAAFSLGGA
                     WLAKREGLSAWRRLRRALEEGRVPTAEAADGALILLAGALLLTPGFLTDVAGVLLLLP
                     PVRAAVRRATPRLAAWRLRRAGGRRGGARTRVRVQVVDGVAYPPGSRTPGSPPAGPPG
                     TSASWGGLELDERSPSGPPS"
     gene            complement(1363..1842)
                     /locus_tag="VEP73_11300"
     CDS             complement(1363..1842)
                     /locus_tag="VEP73_11300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HYT27056.1"
                     /translation="MTSAPPHAGAGGHGEHEVAVWTDGGARGNPGPAGIGAVVARPDG
                     TVLAEVAEGIGWATNNVAEYRAVIAGLERARALGARRVRVRADSLLAMRQLSGAWKVR
                     EAALKPLWAEAMRLARGFEQVTFEHVPRERNRAADALANRAMDAQGDVAGPVRGRPG"
     gene            complement(1839..>2523)
                     /locus_tag="VEP73_11305"
     CDS             complement(1839..>2523)
                     /locus_tag="VEP73_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HYT27057.1"
                     /translation="THDSAIDRLDHRREHLPEHAQLAELATTLAAIDQLTAERQGSLA
                     TVRREHDRLEAEIDMVTRKAASEEARAASGRVTSPKELTAIQDEVAALHRRQSTLEDD
                     LLERMEARETLERELAELASRREAVVSEQAKVGQARDTALAEIDRQLEVERAARAVVA
                     PTVDERLAALYDQIRRRQGGVGAAALVGNTCQGCRVTISPVELNAIRKLPAEEIKRCE
                     NCRRILVVG"
BASE COUNT          242 a          992 c          921 g          368 t
ORIGIN      
        1 cagagagctg cgagacccgc tcggccccga ccgcctcgcg cagctcggcc aggtccgggg
       61 cgggcaccgc aagccagtcc gcggtggtgc cgaacgcgtc ctcgaagccc tcgccggcga
      121 gcttggcgag gacggtgacg cgcaccgccg gcgacaccgg cgggaacggt gggttcgtgt
      181 ccacgcgcct ccttcggctg gggcaccggc gccgagcagc cacccttcat gaccgaatgg
      241 tgcagaagga gccggcctgt aagccggctt ctgtctcccc cggtgtcccc ggggggaggc
      301 ggccatccat ctgggaccgg tgtcgccacc ggcctcgtgc ggcctaccag gggactcgac
      361 cgggccggtc tcctcggccc gcctggcggc gggccgtacg tccccaactt ggccttgctc
      421 cgggtggggt ttgccgagcc gaccggtcac ccggccgctg gtgcgctctt accgcaccgt
      481 ttcacccttg ccgcgggcgc tggaccctcg acgggccccg gcgcgtgctc gccgcgagcg
      541 gcgaggcggc ggtctgttct ctgtggcact ttcctgcggg tcgccccgac tggccgttag
      601 ccagcaccct gccctgcgga ggccggactt tcctcgaccc cggtcgcccg gggccgcggc
      661 cgcccggccg gctccctctg cccctccagt atgccaggcg gcacgtccag cctgtcggcc
      721 gggcccgccg tccggctccg ggtcgggcat actggatgga gctactgagc tcaactgagg
      781 tttgaccatg cttcctgccc tcgtccttgc cttcatcgtc gtccccatcg ccgagctggc
      841 cgtgatcctg gccatcggcc acagcatcgg gttcctgccc accctcctcc tcgtggcggc
      901 gttcagcctc ggcggcgcct ggctggccaa gcgggagggc ctgtcggcgt ggcggcgcct
      961 gcggcgcgcg ctcgaagagg gccgcgtgcc caccgccgag gcggccgacg gggcgctgat
     1021 cctgctcgcc ggcgccctgc tcctgacccc ggggttcctc accgacgtgg cgggggtgct
     1081 cctgctgctc ccgcccgtgc gggccgccgt ccgccgggcc acgcccaggc tcgccgcgtg
     1141 gcggctgcgc cgggccggcg gccgccgtgg tggcgcccgg acccgggtgc gggtccaggt
     1201 cgtcgacggc gtcgcctacc cgcccgggtc gcgcacgccc ggatccccgc cggccgggcc
     1261 tcccggaacg agcgcctcct ggggcgggct cgagctcgac gaacgctcgc cgtcgggccc
     1321 gccgagctga ggccgccgcg gcgggtcggg gtcgggtcgg ggctacccgg ggcgcccccg
     1381 gaccgggccc gcaacgtcac cctgggcgtc catcgccctg ttggcgagcg cgtcggcggc
     1441 ccggttgcgc tcgcgcggca cgtgctcgaa cgtcacctgc tcgaagccgc gcgccaggcg
     1501 catcgcctcg gcccacagcg gcttcagggc ggcctccctc accttccacg cgccgctgag
     1561 ctgccgcatc gcgagcagcg agtcggcgcg cacccgcaca cggcgggcgc cgagcgccct
     1621 ggcacgctcc agcccggcga tcacggcccg gtactcggcg acgttgttcg tcgcccaccc
     1681 gatcccctcg gcgacctcgg cgaggacggt cccgtcgggc cgcgcgacga cggcgccgat
     1741 cccggccggc cccgggttgc ccctggcgcc gccgtcggtc cagaccgcca cctcgtgctc
     1801 gccgtgcccg cccgctccgg cgtggggcgg ggcggaggtc aaccgaccac caggattcgc
     1861 cggcagttct cgcaccgctt gatctcctcg gcgggcagct tgcggatggc gttcagctcg
     1921 accggcgaga tcgtcacccg gcagccctgg caggtgttgc cgaccagggc ggccgccccg
     1981 acacccccct gcctgcgccg gatctggtcg tagagggcgg cgaggcgctc gtcgaccgtt
     2041 ggcgcgacca ccgcgcgcgc ggcccgctcg acctcgagct gccggtcgat ctcggccagg
     2101 gccgtgtcgc gggcctggcc gaccttcgcc tgctcggaga ccaccgcctc gcgccggctc
     2161 gcgagctcgg cgagctcgcg ctccagggtc tcgcgcgcct ccatgcgctc gagcaggtcg
     2221 tcttcgaggg tcgactgcct gcggtgcagg gcggcgacct cgtcctggat cgcggtgagc
     2281 tccttcgggc tggtcacccg gcccgaggcc gcccgcgcct cctcgctggc cgccttgcgg
     2341 gtcaccatgt cgatctcggc ctcgaggcgg tcgtgctcgc gcctgacggt ggccagggag
     2401 ccctggcgct cggcggtgag ctggtcgatt gcggccaggg tggtggcgag ctcggcgagc
     2461 tgggcgtgct cgggaaggtg ctcgcgccgg tggtcgagac ggtcgatggc cgagtcgtgg
     2521 gtc
//
LOCUS       DASPYY010000721         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_397123, whole genome shotgun sequence.
ACCESSION   DASPYY010000721 DASPYY010000000
VERSION     DASPYY010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397123"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VEP73_11310"
     CDS             <1..266
                     /locus_tag="VEP73_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT27058.1"
                     /translation="DVRSRVTGAFQAVNYGTRPLGALLGGFLGSPWLLGPRVTLWVAA
                     IGGMTSFLVLLPTQIPRFRMPAASGGRETPALPDAQDLANSAA"
     gene            complement(316..1380)
                     /gene="alc"
                     /locus_tag="VEP73_11315"
     CDS             complement(316..1380)
                     /gene="alc"
                     /locus_tag="VEP73_11315"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HYT27059.1"
                     /translation="MRGGQVAGDFTRLADLASRRLGGSVVYANDELFAEKENLIKPEP
                     PSFAEGDFGNKGKVYDGWETRRRRDPGHDHAIVRLGCPGVVHGVVVDTAFFRGNFAPE
                     ISVEAASVTGYPGPAELAGVAWHPLVAKSAATGDTSNSYPVADPRRWSHVRLSIYPDG
                     GVARLRVHGEVVPDPAFLTGTADLAAMENGGRVIACSDAFYSSPSNLIMPGLARVMGD
                     GWENARRRGPGNDFVAFRLAAAGRLRHVEIDTSYFVGNAPGWIELSGASAGPPSGDEP
                     PGPSRADGEEDLAWRPVLARTRVQPDTTHRFLIGEPDDVTHLRLDVFPDGGLARFRAY
                     GEVTAEGSADLARRWRETAP"
     gene            complement(1377..2834)
                     /gene="allB"
                     /locus_tag="VEP73_11320"
     CDS             complement(1377..2834)
                     /gene="allB"
                     /locus_tag="VEP73_11320"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HYT27060.1"
                     /translation="MPAEPPQAATAGPPPDLIIRANRAVTPAGVVPAEVHIRDGRITA
                     VTALDDRTAVPRGPRSAGPGAPAARVIPPSEVVNSPAEHIELTDDEVLMPGLVDTHVH
                     VNEPGRTDWEGFATATAAAAAGGVTTIIDMPLNSIPPTIDAAALDAKRAGAEGQCAVD
                     VGFWGGAVPSNAADRPGLHEAGVFGFKCFMVDSGVPEFQPLDDHGLGAAMKQVASLGS
                     LLIVHAEDPASITTAPAPSGGDYAGFLRSRPPAAEVAAIITVLRLAVATGARVHVLHL
                     SSADPVPLLTAAREFGAQVSAETCPHYLTLAAEQVGTGRTEFKCCPPIRGVDNRERLW
                     AALESGAIDCVVSDHSPCPPALKDLATGDFGTAWGGISSVQLGLPVVWTHARERGFGL
                     ADLARWMSASPASLAGLAAKGAIQVGRDADLISFAPDEQFVVTPGQLLTRHKLTPYAG
                     QELSGVVRRTWLRGVPVSPGRPPRGRLLRRAGEQR"
     gene            complement(2835..>3501)
                     /locus_tag="VEP73_11325"
     CDS             complement(2835..>3501)
                     /locus_tag="VEP73_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HYT27061.1"
                     /translation="EESFSVRQPRGVAFEIQVETPQAILGADGAATIAKCVHAAPGRL
                     TGLHYGTYDYTAALGIAAPFQSMEHPAADHAKVVMQVAAAGTGVRLSDGSTNVLPVGT
                     RDQVIAAWRLHSRLVRRSLERGFYQGWDLHPAQLVTRYAATYAFYREGLPAAFARLRG
                     AAERSASGQEEPATIRALSGFLARALDCGALTEHELAATTGLDSHQLRVARRRGIVPD
                     RGP"
BASE COUNT          452 a         1233 c         1326 g          490 t
ORIGIN      
        1 acgacgtgcg ttcccgggtg accggagcgt tccaggcggt gaactacggc accaggccgc
       61 tcggcgcgtt gctcggcggc ttcctcggct ccccgtggct cctcggaccg cgcgtcacgc
      121 tctgggtggc ggcgatcggc gggatgacgt ccttcctcgt gctgctgccg acccagatcc
      181 cgaggttccg gatgccggcc gcatccggcg ggcgcgagac tccggcgctg ccggatgcgc
      241 aggatctcgc gaacagcgcg gcctgacttc cggctacagt gcggtgtgcc gcggctacgg
      301 tgcggtgtgc cgcggctacg gtgcggtttc ccgccatcgc ctggcgaggt cggccgaccc
      361 ctcggcggtg acctcgccat aggcacggaa cctcgcgagc ccgccgtcgg gaaagacgtc
      421 gagccgcagg tgggtgacgt cgtccggctc gccgatcagg aagcggtgcg tggtgtccgg
      481 ctgcacgcga gtccgcgcca gcaccggccg ccaggccagg tcctcctcgc cgtccgctcg
      541 ggagggtcct gggggctcgt ccccgctggg tggaccagcg ctggcgccag acagctcgat
      601 ccagcctggc gcgttgccga cgaagtacga ggtgtcgatc tccacgtgcc gcagccgacc
      661 ggcggcggcc agccggaagg cgacgaagtc gttgcccggg ccgcggcgac gggcgttctc
      721 ccagccgtcg cccatcaccc gggcgagccc gggcatgatc aggttcgagg gcgaggagta
      781 gaacgcgtcc gaacaggcga tcacccgtcc gccgttctcc atcgcggcca gatcggcggt
      841 tccggtcagg aacgcgggat cgggcacgac ctcgccatgc acccgcagcc gggcgacgcc
      901 gccgtcgggg tagatcgaca gcctgacgtg gctccaccgg cgcgggtcgg cgaccgggta
      961 gctgttggat gtgtcgcccg tggcggccga cttcgcgacc agcgggtgcc aggccacgcc
     1021 ggcgagctcg gccgggccag ggtatccggt cacgctcgcg gcctcgaccg agatctccgg
     1081 cgcgaagttg ccgcggaaga acgccgtgtc gaccaccacg ccgtgcacga caccgggaca
     1141 cccgagcctg acgatcgcgt ggtcgtggcc cgggtcgcgg cggcgcctgg tctcccagcc
     1201 gtcgtagacc ttgcccttat tgccgaagtc gccctcggcg aacgacggcg gctccggctt
     1261 gatcaggttc tccttctcgg cgaagagctc gtcgttggcg tagaccacgc tcccgcccag
     1321 ccgccgcgaa gccagatccg cgagcctcgt aaaatctccg gccacctgtc ctcccctcac
     1381 cgttgttctc ccgcgcggcg cagcagccgt ccgcgcggcg ggcggccagg gctgacgggg
     1441 acgccgcgga gccaggtgcg gcggaccacg ccggatagct cctgtcccgc gtacggggtc
     1501 agtttgtgcc tggtcagaag ctggccaggg gtaacgacga actgctcgtc gggcgcgaac
     1561 gagatcaggt cggcgtcgcg gccgacctgg atcgctccct tggcggcgag cccggccagg
     1621 ctggccgggc tcgccgacat ccaccgggcc agatcagcca ggccgaagcc gcgctccctg
     1681 gcgtgggtcc agaccaccgg caagccgagc tggaccgacg agatcccgcc ccacgcggtc
     1741 ccgaagtcgc cggtggccag gtccttcagg gcgggcgggc agggtgagtg gtcggacacc
     1801 acgcagtcga tcgcgccgga ctccagcgcc gcccagagcc gctcgcggtt gtccacgccc
     1861 ctgatgggcg ggcagcactt gaactcggtc cggccggtgc cgacctgctc agcggcgagg
     1921 gtcaggtagt gcgggcaggt ctcggcgctg acctgcgcgc cgaactcccg ggcggcggtc
     1981 agcagcggga ccgggtcggc gctggacagg tgcagcacgt gcacccgcgc gccggtcgcg
     2041 acggccagcc gcagcaccgt gatgatcgcc gcgacctcgg cagccggcgg cctggaccgc
     2101 aagaaccccg cgtagtcgcc gccggacggc gccggggcgg tcgtgatgct cgccgggtcc
     2161 tcggcgtgca cgatcagcag gctgccgagg ctcgcgacct gcttcatcgc ggcgccgagg
     2221 ccatggtcgt ccagcggctg gaactccggc acgcccgagt cgaccatgaa gcacttgaag
     2281 ccgaacacgc ccgcctcgtg cagaccaggc cggtcggccg cgttcgaggg gaccgcgccg
     2341 ccccagaacc cgacgtccac ggcgcactgc ccttcggcgc cggcacgctt ggcgtcgagc
     2401 gcagcggcgt cgatggtcgg cgggatcgag ttcagcggca tgtcgatgat cgtcgtgacg
     2461 ccgccggccg cggccgccgc ggtcgccgtc gcgaatcctt cccagtccgt gcggccgggc
     2521 tcgttgacgt ggacgtgggt gtcgacgagg ccgggcatga gcacctcgtc gtcggtcagc
     2581 tcgatgtgtt ccgcggggct gttgacaacc tcgctcgggg ggatgacccg ggcagcgggc
     2641 gcgcccggtc cggctgaacg cggcccgcgc ggcaccgccg tccggtcatc gagtgccgtc
     2701 acggcggtga tgcggccgtc gcggatgtgc acctctgcgg gcacgacgcc ggccggggtt
     2761 actgcccggt tcgcgcggat gatgaggtcg ggcggggggc ccgccgttgc cgcctgcggc
     2821 ggctcggcgg gcatttaggg gccccgatcg gggactatgc cgcggcggcg ggcgacccgt
     2881 agctggtgcg aatccaggcc cgttgtcgcg gcgagctcgt gctcggtgag ggcgccgcag
     2941 tcgagggcgc gggcgaggaa gccggatagg gcgcggatgg tggctggttc ttcctggccg
     3001 gaggcggagc gttcggcggc gccgcggagc cgggcgaagg ctgcggggag gccctcgcgg
     3061 taaaaggcgt aggtcgcggc gtagcgggtg accagctggg cggggtgcag gtcccagccc
     3121 tggtagaagc cgcgttccag ggaccgcctg accaggcggg agtgcaggcg ccaggcggcg
     3181 atgacctggt cgcgggtacc gaccgggagc acgttggtcg agccgtcgct gagccggacg
     3241 ccggtgccgg ccgccgcgac ctgcatgacc accttggcgt ggtcggcagc cgggtgctcc
     3301 atgctctgga acggggcggc gatgccgagc gcggcggtgt agtcgtaggt gccgtagtgc
     3361 agtccggtca gccggccggg cgccgcgtgc acgcacttcg cgatcgtcgc cgcgccgtcg
     3421 gcgccgagga tggcctgcgg cgtctcgacc tggatctcga acgccacgcc ccgcggctgc
     3481 cgcacgctaa acgactcctc g
//
LOCUS       DASPYY010000722         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_258484, whole genome shotgun sequence.
ACCESSION   DASPYY010000722 DASPYY010000000
VERSION     DASPYY010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258484"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VEP73_11330"
     CDS             <1..843
                     /locus_tag="VEP73_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYT27062.1"
                     /translation="PSEALYPLRVLAGALVVYGAATIAHGSGFLAVFVAGILAGDARA
                     PYKGEIERFHAALASLAEIVAFIVLGLTVGLYTLPRSGAWLIGLTLAVLLTVVVRPLL
                     LGLLLLPVRLARGERLFVLWAGLKGAVPVLLGTFVITAGVPDAPRIYSVIVVVVAFSV
                     IVQGGLVPAVATRLGVPMRAIEPEPWSLGVRFRHEPRGLRRHVVAPGSPADGCTIGDL
                     ALGEDVWISLVIRDGQLVPVRSSTPLRAGDEVVALADPERAPDLAPVFTAGQGTPDTG
                     STPP"
     gene            complement(913..1425)
                     /locus_tag="VEP73_11335"
     CDS             complement(913..1425)
                     /locus_tag="VEP73_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27063.1"
                     /translation="MPRFGPNLFGEVLEPSRVSNVRLGIVTDQPVPAIPLVPGSILLV
                     APPGLLHPKVRQLDGGLLYDLVTGHPSRTPGDVVFLADLTQELWAWDGSTWAALGVDP
                     ARVGDMIALRYQHGDVDALRIGDLTADELRALDQLPMAARRDELRRRLRDRVAAHATP
                     RPRQPGAHRA"
     gene            1840..2241
                     /locus_tag="VEP73_11340"
     CDS             1840..2241
                     /locus_tag="VEP73_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYT27064.1"
                     /translation="MQTYRIGRVAELLGVSVDTVRRWTDSGRLVSHRTKHGQRLIDGA
                     SLARFLTETAPAAEHGSIVAASARNRFTGIVTRVVKDTVVAQIEVRSGPHRLVSLMTA
                     EAADELGLAPGVLAVAAVKATNVVVEVPSPD"
     gene            2284..3066
                     /gene="modA"
                     /locus_tag="VEP73_11345"
     CDS             2284..3066
                     /gene="modA"
                     /locus_tag="VEP73_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYT27065.1"
                     /translation="MGPSRTRPAVALVLVALAAAGCGGSGGPATGRASRPSTEIKVFA
                     AASLTAAFGKLGQQFTAAHPETRVTFSFAGSQALATQIEQGAPADVFAPADTGNMTRV
                     AGLVATPQSFASNLLGIVVAAGNPKGVRGLADLAREDLKVVLAAPEVPAGRYARQALD
                     ARHVTVRPVSLEDNVKAVVGKVSLGEADAGIVYVTDVAAAGGKVEGVDIPREQNVPAT
                     YPIAVVKAGKHQQQAQGFVDLVRSPAGQQVLKSYGFLPPPTS"
     gene            3063..3878
                     /locus_tag="VEP73_11350"
     CDS             3063..3878
                     /locus_tag="VEP73_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020137114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT27066.1"
                     /translation="MTGTARRRRSGASAGAPIVFVVPAALAVGFLAVPLLGLLDQTPW
                     RHLAAELAAPQVRVALRLSLVCSLGAVAISVLLGVPLAWLLARVDFPGRNLVRALVAL
                     PMVLPPVVGGVALLLAFGRRGLVGLWLDRAFGITLPFTTMGAILAEAFVAMPFLVITT
                     EAGLRAMDRRFEDAAATLGAGRWTTFRRVTLPLVAPSLGAGSALCWARALGEFGATIT
                     FAGNLPGSTQTMPLAVYLALQNDLQAAIALSLVLLVISLAVLVALRDRWFPAP"
BASE COUNT          427 a         1497 c         1474 g          571 t
ORIGIN      
        1 cccagcgagg cgctctaccc gctgcgcgtc ctggccggcg ccctggtcgt ctacggcgcg
       61 gcgaccatcg cgcacggctc cggcttcctg gccgtgttcg tcgccggcat cctggccggg
      121 gacgcccgcg ccccgtacaa gggcgagatc gagcggttcc acgccgccct ggcgagcctc
      181 gccgagatcg tcgccttcat cgtgctcggt ctgaccgtcg gcctgtacac gctccctcgc
      241 agcggcgcgt ggctcatcgg cctcacgctg gccgtgctgc tcacggtcgt cgtccgccca
      301 ctgctcctcg gcctgctgct gctgccggtc cggctggccc ggggcgagcg gctgttcgtg
      361 ctgtgggccg ggctcaaggg cgccgtcccg gtcctgctcg gcaccttcgt gatcaccgcc
      421 ggcgtgcccg acgccccccg gatctactcc gtcatcgtcg tggtcgtggc cttctcggtc
      481 atcgtgcagg gcgggctcgt gcccgccgtc gcgacccggc tcggtgtgcc gatgcgcgcc
      541 atcgagccag agccgtggag cctcggcgtg cggttccgcc acgagccgcg cgggctgcgc
      601 cgccacgtcg tggctcccgg ctcgccggcg gacgggtgca cgatcggtga cctcgccctc
      661 ggggaggacg tctggatcag cctggtcatc cgcgacggcc agctcgttcc cgtccggagc
      721 tccaccccgc tgcgcgccgg tgacgaggtc gtcgcgctgg ccgaccccga gcgcgccccg
      781 gacctggcac ctgtcttcac cgccgggcag ggcacgcccg acacgggctc cacgccgccc
      841 tgacggccgg tcggcccgcg gccggggacc gtcgccgtcc gtcgacgccg gatccggcgg
      901 gccggagcgg cgtcatgccc ggtgggcgcc tggctggcgt ggtcgcggtg tggcgtgcgc
      961 ggcgacgcgg tcgcggaggc ggcggcggag ctcgtcgcgc cgcgcggcca tcgggagctg
     1021 gtcgagcgcc cggagctcgt cggcggtgag gtcgccgatc cggagggcgt cgacgtcgcc
     1081 gtgctggtag cgcagggcga tcatgtcgcc gacccgggcg gggtcgacgc cgagggcggc
     1141 ccaggtggag ccgtcccagg cccacagctc ctgggtgagg tcggcgagga agacgacgtc
     1201 gccgggggtg cggctggggt ggcccgtcac caggtcgtac agcagcccgc cgtcgagctg
     1261 gcggaccttg gggtgcagca gcccgggcgg cgcgaccagc aggatgctgc cgggcacgag
     1321 cgggatggcg gggacgggct ggtcggtgac gatcccgagg cgcacgttgc tgacccggct
     1381 cggctcgagg acttcgccga acaggttggg gccgaagcgc ggcattctcg tgatcctttc
     1441 gggaaacgga cggtgaccgg cagcaccggg tcggccatgg tcgcaccgcc ggctggcggc
     1501 ggcacggtgt cgcgacgagc tgtgggggcg gtgcgccatg ccgtcatggc tggctcctgg
     1561 caagggacgg acctgccggc caggctcccg gcacaccacg ccggagccta ccgaggtccg
     1621 cgcccgcctg tcacctgcgt gcggtcaaca cggccggtcc agccggaccg ggcgcggccg
     1681 tgccacccgg ccggcacgcc cggcacccgt ggcccgcggc ctcgccagcc tggcagacgc
     1741 ctcgcagaag ccaggatgtt cccggctcgc gcgggcgcct gcggccttcg agaggtctaa
     1801 taccttgcac atgcgctgtc agcctggttg agtggcgtca tgcagacgta ccggatcggc
     1861 cgcgtcgccg agctgctcgg cgtgagcgtc gacaccgtgc ggcgctggac cgacagcggc
     1921 cggctggtga gccaccgcac caaacacggc cagcgcctga tcgacggtgc cagccttgcc
     1981 cgcttcctga ccgagaccgc cccggcggcg gagcatggct cgatcgtggc cgcgtccgcc
     2041 cggaaccgct tcaccggcat cgtcacccgg gtcgtcaagg acacggtcgt cgcgcagatc
     2101 gaggtgcggt ccggaccgca ccggctggtg tcgctgatga ccgcggaggc ggccgacgag
     2161 ctcggcctcg cgcccggtgt cctcgcggtc gcggccgtca aggcgaccaa cgtggtcgtc
     2221 gaggtcccgt cccccgacta ggagctcgag gtcccgtccc ggaccaggag gaggcagccg
     2281 gccatgggcc cgtcccgcac ccgccctgcc gtcgcgctcg tgctggtggc gctggccgcc
     2341 gccggctgcg gcggcagcgg cgggcccgcg accggtcgcg cctcgcggcc gtcgaccgag
     2401 atcaaggtgt tcgcggccgc gtcgctcacc gccgcgttcg gcaagctcgg ccagcagttc
     2461 accgccgcgc atcccgagac cagggtcacc ttcagcttcg ccggcagcca ggccctcgcg
     2521 acccagatcg agcagggcgc ccccgccgac gtgttcgccc cggccgacac cgggaacatg
     2581 accagggtcg ccggcctggt cgccaccccg cagagcttcg cgagcaacct gctcgggatc
     2641 gtcgtcgccg cgggcaaccc caagggcgtc aggggcctcg ccgacctggc ccgcgaggac
     2701 ctgaaggtgg tgctcgcggc gccggaggtc ccggcgggca ggtacgccag gcaggccctt
     2761 gacgcccggc acgtcaccgt ccggccggtg tcgctcgagg acaacgtcaa ggcggtggtc
     2821 ggcaaggtct ccctcggcga ggccgacgcc ggcatcgtct acgtgaccga cgtcgccgcc
     2881 gccgggggca aggtcgaggg cgtcgacatc ccgcgggagc agaacgtccc ggcgacctac
     2941 ccgatcgcgg tcgtcaaggc gggcaagcac cagcagcagg cgcaagggtt cgtcgacctg
     3001 gtccgctcgc cagcgggcca gcaggtcctc aagagctacg ggttcctccc gccgccgacg
     3061 tcgtgaccgg cacggctcgg cgtcgccggt cgggggcctc cgccggcgcc cccatcgtct
     3121 tcgtcgtgcc tgccgcgctc gccgtgggct tcctggccgt cccgctgctc ggcctgctgg
     3181 accagacgcc ctggcggcac ctggccgccg agctggccgc gccgcaggtg cgggtcgcgc
     3241 tgcggctgtc gctggtctgc tcgctcggcg ccgtcgccat ctcggtgctg ctcggcgtcc
     3301 ccttggcgtg gctgctcgcg cgggtggact tcccgggccg caacctggtg cgcgcgctcg
     3361 tcgccctgcc gatggtgctg ccccccgtgg tcggcggcgt ggcgctgctg ctcgcgttcg
     3421 gccggcgggg actggtcggc ctgtggctgg accgcgcctt cgggatcacg ctgccgttca
     3481 ccaccatggg cgcgatcctc gccgaggcgt tcgttgccat gccgttcctg gtcatcacca
     3541 ccgaggcggg cctgcgggcg atggaccgcc gcttcgagga cgcggccgcc accctcggcg
     3601 ccggacggtg gacgacgttc cggcgggtga cgctgccgct cgtcgccccg tcgctcggcg
     3661 cgggcagcgc gctgtgctgg gcccgggcgc ttggcgagtt cggcgcgacg atcacgttcg
     3721 ccggcaacct gcccggctcg acccagacca tgccgctcgc ggtctacctc gccctgcaga
     3781 acgacctcca ggccgccatc gcgctcagcc tggtcctgct ggtgatctcc ctggccgtgc
     3841 tggtcgccct gcgggaccgc tggttcccgg ccccgtgagc ctcgaggccc gggtcgggct
     3901 caccctcggc cagctcgggc tggacgtcga gctggcggtg gcgaccgggg agctggtggt
     3961 gctgctcgg
//
LOCUS       DASPYY010000723         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1524307, whole genome shotgun sequence.
ACCESSION   DASPYY010000723 DASPYY010000000
VERSION     DASPYY010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524307"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VEP73_11355"
     CDS             complement(<1..486)
                     /locus_tag="VEP73_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYT27067.1"
                     /translation="MFCTQCGHKNPEGAHFCANCGAALVAPADDSTTTISLAAQDLEA
                     EVEEEVAAPVEELREGTAMLVVRRGPNAGSRFLLDKEVVTAGRHPESDIFLDDITVSR
                     RHAEIRRSGTGFTVHDVGSLNGTYLNRERVEDGDLVGGDELQIGKFKLVFFAGGRPGA
                     GG"
     gene            complement(629..1009)
                     /gene="gcvH"
                     /locus_tag="VEP73_11360"
     CDS             complement(629..1009)
                     /gene="gcvH"
                     /locus_tag="VEP73_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HYT27068.1"
                     /translation="MYPDDLKYTPAHEWARVEGGRATVGITHYAQDALGDVVYADVPP
                     VGTAVTSGEPFGEVESTKSVSDIYSPVSGTIAARNDELDKSPEIINSDPYGQGWLVVI
                     EMSDPGQADDLMDADAYRELVAET"
     gene            complement(1042..2835)
                     /locus_tag="VEP73_11365"
     CDS             complement(1042..2835)
                     /locus_tag="VEP73_11365"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HYT27069.1"
                     /translation="MLARPAPYTPGGPTRGRLLGLPVPAAAALLALAVVLALVGADGA
                     LAAGRVRRGVHLGGVDLSGLTRAQARARLAAAAPAVEARPVTVRADTVTVGLPRSATG
                     VRLDVDASVAAALAVGRSGPADPDRLRTWLTGVDLPWRTRLDESAFGRVVARLDRQVR
                     RPVREPRLVVGTSGARLVRGVPGRAVDRAAAVRALGAAAARPSVAEITLPVTEQRPTV
                     GQAAAEAAERQAGALLSAPVLVAAAGRQGSLSPADLAARVRTGIRAGRLVVRLDPGEL
                     DGLLRRRAAFAYATPRDAGFRAAGGRVAVVPAVEGSVVDPAGAAAAVLAAGGRGGAGR
                     RATLPVRTRGPALTTEKAEALGVKQVISSFTTSFSAADAPRVHNISLIAAAVNGRLVL
                     PGEVFSMNGATGERTAAKGYRTAHVIQNGEVVDGLGGGVCQAGTTMFNAVFFAGLPVV
                     ERRNHSLHISHYPMGRDATLNWPGTDLKFRNDSPYGIYITARTTPATMNVTLYSTSRR
                     YKVTSSTSAPYDYRPPQVKYTDDPTLPAGTEVVESQGSPGFDVTVRRTVTQDGRVVRR
                     DTFVSSYIPWSKVIRRGATQPAAPTTTPPPA"
     gene            complement(3150..>4581)
                     /locus_tag="VEP73_11370"
     CDS             complement(3150..>4581)
                     /locus_tag="VEP73_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HYT27070.1"
                     /translation="KSIIWEGRGARTLFSEHGVTGLANIDVTPELALRVATAFGSTLK
                     KGSRVTLGRDASRVSRALKRVLVAGLNATGIHCDDLELAPAPAVRFSATRGGASGGIY
                     VRTSPRDGLAVEIAVFGDDGSDLDEVARRKVERTYNREEFRRAFGREMGELRYPPRAI
                     EHYTAALVDCVDARAVSHRRFKVVIDAGAGTCSLVLPWLLPRFGLDSLLVGGQLDEER
                     ASPSAAQVAEDLDRLARLVTASGADLGVRFDTVGERLTLVDSGGRVVPHHQALLLFVD
                     LVVRSEQPGTVALPVSTTRRAAELAARHGCEVLGTKLTGSAIMRASQGDGVVFAGAEG
                     GGYVVPALHPAYDGLVSFAKLLELLAAQEVSLAEAVAALPEACVVRRRVPTPWEQKGA
                     VMRQVVEAAKGHRTQDVDGIKVFHPGAPAGGGAHEGGPDDDWVLVLPDTTEPVTHLWA
                     EAATREGADALADRYEALVKRAIDGG"
BASE COUNT          568 a         1703 c         1733 g          577 t
ORIGIN      
        1 tccaccggct cccgggcggc cgcccgcgaa gaacacgagc ttgaacttgc cgatctgcag
       61 ctcgtcacct ccgaccaggt cgccgtcctc gacccgctcg cggttcaggt acgtgccgtt
      121 cagggagccg acgtcgtgca ccgtgaagcc ggtgccggag cggcggatct ccgcgtgccg
      181 gcgggagacc gtgatgtcgt cgaggaagat gtcgctctcg gggtgccgcc ccgcggtcac
      241 gacctccttg tcgagcagga agcggctgcc ggcgttcggg cccctgcgca ccacgagcat
      301 ggccgtgccc tcccggagct cctcgaccgg ggcggcgacc tcctcctcca cctcagcttc
      361 gaggtcctgg gcggcgaggc tgatcgtggt cgtcgagtcg tcagcgggtg ccaccagcgc
      421 ggcgccacaa ttcgcgcaga agtgggcgcc ttccgggttc ttgtgaccgc actgggtgca
      481 gaacattccg ccctccgggc tatggctcga tacggacgct gactgtagag gacccgctga
      541 aatcgggtca acgcaacgtg cctggtcgga cgtgccgcga acccggccgg cacggaccgc
      601 cagaaccctc gacctcaggg tcgcctgctc aggtctccgc caccagttca cgatacgcgt
      661 cggcatccat gaggtcgtcg gcctggccag ggtcgctcat ctcgatcacg accagccagc
      721 cctgcccgta cgggtcggag ttgatgatct cggggctctt gtcgagctcg tcgttgcggg
      781 cggcgatcgt cccgctcacg gggctgtaga tgtcggagac cgacttcgtc gactcgacct
      841 ccccgaaggg ctccccgctg gtgaccgccg tgccgaccgg cgggacgtcg gcgtacacga
      901 cgtcgccgag cgcgtcctgc gcgtagtggg tgatccccac ggtggcccgg ccgccctcga
      961 cccgggccca ctcgtgggcc ggggtgtact tcaggtcgtc agggtacatg tcggttcctc
     1021 cgcgcctcgg tgtcccgccg gtcaggcggg cggcggcgtg gtcgtcggtg cggcgggctg
     1081 cgtcgcgccc cgccggatga ccttgctcca cgggatgtag ctggagacga acgtgtccct
     1141 gcgcacgacg cggccgtcct gggtgacggt ccggcggacg gtcacgtcga acccggggga
     1201 tccctggctc tcgacgacct cggtgcccgc gggcagggtc gggtcgtcgg tgtacttcac
     1261 ctggggggga cggtagtcgt agggcgccga ggtgctcgag gtgaccttgt accgccgcga
     1321 ggtggagtac agggtgacgt tcatggtggc cggggtcgtc ctggccgtga tgtagatccc
     1381 gtacggggag tcgttgcgga acttgaggtc ggtgcccggc cagttgaggg tcgcgtcccg
     1441 ccccatgggg tagtggctga tgtgcaggct gtggttgcgg cgctcgacga ccggcaggcc
     1501 ggcgaagaag accgcgttga acatcgtcgt ccccgcctgg cacacgccgc cacccagccc
     1561 gtcgacgacc tcgccgttct ggatgacgtg ggcggtccgg tagcccttgg ccgcggtgcg
     1621 ctcgccggtc gcgccgttca tcgagaacac ctcgccgggc aggacgagcc ggccgttcac
     1681 ggccgcggcg atcagcgaga tgttgtgcac gcggggcgcg tcggccgcgc tgaacgaggt
     1741 ggtgaacgag gagatgacct gcttcacccc gagcgcctcc gccttctcgg tggtgagcgc
     1801 ggggccgcgg gtgcgcaccg gcagcgtggc ccggcggccc gccccgcccc gcccgcccgc
     1861 ggccagcacg gccgcggccg cccccgccgg gtcgacgacg ctgccctcga ccgccgggac
     1921 cacggcgacc cgcccgccgg cggcccggaa gccggcgtcc cgcggggtcg cgtaggcgaa
     1981 ggccgcccgc cggcggagca ggccgtcgag ctccccgggg tcgaggcgga ccaccaggcg
     2041 gccggcccgg atgccggtcc ggacgcgcgc ggccaggtcg gccggggaca gggagccctg
     2101 ccggcccgcc gcggccacca ggaccggggc ggacagcagg gcgccggcct ggcgctcggc
     2161 cgcctcggcg gccgcctggc cgacggtcgg gcgctgctcg gtcacgggca gggtgatctc
     2221 ggccaccgac ggccgggcgg ccgcggcccc gagcgcccgc accgcggcgg cccggtccac
     2281 cgcccgcccc ggcaccccgc gcacgagcct ggccccggag gtgccgacga cgagccgcgg
     2341 ctcgcggacc ggacggcgca cctggcggtc gagcctggcc acgacccgcc cgaacgcgct
     2401 ctcgtccagg cgcgtccgcc aggggaggtc gacgccggtg agccaggtcc gcagccggtc
     2461 ggggtcggcc ggcccggagc ggcccacggc cagggcggcg gccacgctcg cgtcgacgtc
     2521 gagccgcacg cccgtggccg agcggggcag gcccacggtc acggtgtcgg ccctgaccgt
     2581 caccggccgc gcctcgacgg ccggggcggc cgcggccagg cgcgcccggg cctgggcgcg
     2641 ggtgaggccg gagaggtcca cgccgcccag gtggaccccg cggcggaccc tgccggcggc
     2701 cagcgcgccg tcggcgccca cgagcgcgag cacgacggcg agcgccagga gggcggccgc
     2761 cgcgggaacg ggcagcccga gcaggcggcc acgcgtgggg ccgccgggcg tgtacggcgc
     2821 cggccgggcc agcaccaggg cccgctcctc ggtcggcgca ggcgccggcc ggtctacgac
     2881 gtcgatgctc gctccctcgc tctcctacga ccggcgcgcg ccacggccgg tgcactggga
     2941 cggacgcgga ccccgctgcc cgactcgaca agaaccgcta gcttacgacc tcgacagggc
     3001 agagggggaa gccggcgccg ctcaccggct gcgttgtccg gctgcgtttt caaaggtgtg
     3061 cgcaagagcc tacggccggt cgaccacgcg cgcgacacgc gagggtcacg gcggcgcaac
     3121 gatggccgcg acggcggccc cgccatcgtt catcccccgt cgatcgcccg cttgaccagg
     3181 gcctcgtacc ggtcggcgag ggcgtcggcg ccctcgcggg tggcggcctc cgcccacagg
     3241 tgggtgaccg gctcggtcgt gtcggggagc acgagcaccc agtcgtcgtc gggaccgccc
     3301 tcgtgggcgc cgccaccggc cggcgcgccc gggtggaaca ccttgatgcc gtcgacgtcc
     3361 tgggtgcggt ggcccttggc ggcctcgacg acctggcgca tgaccgcgcc cttctgctcc
     3421 cacggggtgg gcacccggcg gcggacgacg cacgcctccg ggagggcggc gaccgcctcg
     3481 gccaggctca cctcctgggc ggcgagcagc tcgagcagct tggcgaacga caccagcccg
     3541 tcgtaggcgg ggtgcagggc ggggaccacg tagccgccgc cctcggcgcc ggcgaacacc
     3601 accccgtcgc cctgggaggc ccgcatgatc gcgctccccg tgagcttcgt gccgagcacc
     3661 tcgcagccgt gcctggccgc gagctcggcc gcccgcctgg tggtcgacac cggcagggcg
     3721 accgtccccg gctgctcgga ccggacgacc aggtcgacga agaggagcag ggcctggtgg
     3781 tgggggacga cccggccgcc ggagtcgacc agggtgaggc gctcgccgac cgtgtcgaag
     3841 cgcacgccga ggtcggcgcc ggaggccgtc accaggcgcg cgagccggtc caggtcctcg
     3901 gcgacctgcg cggccgacgg cgacgcccgc tcctcgtcga gctgcccgcc gacgagcagg
     3961 gagtcgaggc cgaaccgggg cagcagccag ggaagcacga gcgagcaggt cccggcgccc
     4021 gcgtcgatca ccaccttgaa gcggcggtgg ctgacggccc gcgcgtcgac gcagtcgacg
     4081 agggcggccg tgtagtgctc gatcgcgcgc ggggggtagc gcagctcgcc catctcgcgg
     4141 ccgaaggcgc ggcggaactc ctcccggttg taggtgcgct cgaccttgcg cctggcgacc
     4201 tcgtcgaggt cgctgccgtc gtcgccgaac acggcgatct cgaccgccag gccgtcccgg
     4261 ggcgacgtgc gcacgtagat gcctcccgac gcgccgcccc gggtggccga gaagcgcacc
     4321 gccggggccg gggccagctc caggtcgtcg cagtggatgc cggtggcgtt caggccggcc
     4381 acgagcaccc gcttgagcgc ccgggacacc cggctggcgt cgcgcccgag cgtcaccctc
     4441 gaccccttct tgagggtcga gccgaaggcc gtcgccaccc gcagggcgag ctcgggcgtc
     4501 acgtcgatgt tggcgaggcc ggtcaccccg tgctcggaga agagcgtgcg cgcgccccgc
     4561 ccctcccaga tgatcgactt g
//
LOCUS       DASPYY010000724         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_116703, whole genome shotgun sequence.
ACCESSION   DASPYY010000724 DASPYY010000000
VERSION     DASPYY010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116703"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2197)
                     /locus_tag="VEP73_11375"
     CDS             complement(<1..>2197)
                     /locus_tag="VEP73_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYT27071.1"
                     /translation="VAARLAREVADDFATDGRPRWVAGSMGPGTKLPTLGHTTYGALL
                     ATYADQAYGLLAGGADLLLVETCQDLLQLKAAVAGSHAGMRRAGRKTPLMVQVTVETT
                     GQMLLGSDIAAALTAIEPLGVDVVGMNCATGPAEMGEHLRYLSKHARTPLSCLPNAGL
                     PELRDGAPHYPLGPAELAEAHDRFVAEFGVNVVGGCCGTTPEHVRQVVEAVGGRAPAP
                     REPVHEPSLSSLYQSVPLDQDLTYLTVGERCNANGSRKFRDLMLEGDLEGMTGIAKEQ
                     IREGAHVLDVCVDYVGRDGVPDMDVLAEQLARQSTVPVMLDSTQADVIEAGLARLGGR
                     AIINSINLEDGERRMDAVCPMARQYGAALVALLIDEEGQARDVEWKLRVAHRIHDLAV
                     GKHGLAPDDLVFDALTFPLSSGQEDLRGDAVATLDAIRRIKAELPGVRTILGVSNVSF
                     GLSPAARQVLNSVFLHEAREAGLDAAIVSPAKILPMHRIDEEQREVALDLVYDRRRQG
                     YDPLQRFMALFEGKEATKSATVEDLAALPIDERLQRRIVDGVRDGLEADLAAALETRG
                     ALAIINEVLLAGMKTVGELFGAGEMQLPFVLQSAEVMKAAVAVLEPHMEKADQGGKGR
                     VVLATVKGDVHDIGKNLVDIILTNNGYTVHNIGIKQPISAIVDKAEEIRADAIGMSGL
                     LVKSTVVMRENLEELNARGLAGYPVLLGGAALNRTYVEGDLRQIYKGRV"
BASE COUNT          285 a          780 c          807 g          325 t
ORIGIN      
        1 aacacccggc ccttgtagat ctggcgaagg tcgccctcga cgtaggtgcg gttcagcgcc
       61 gccccgccga gcagcaccgg gtagccggcc agcccgcggg cgttcagctc ctcgaggttc
      121 tcgcgcatga ccacggtcga cttgacgagc agcccggaca tgccgatcgc gtcggcgcgg
      181 atctcctcgg ccttgtcgac gatcgccgag atcggctgct tgatgccgat gttgtggacc
      241 gtgtagccgt tgttggtgag gatgatgtcc acgaggttct tgccgatgtc gtggacgtcg
      301 cccttcacgg tggcgagcac gacccggccc ttgccgccct ggtcggcctt ttccatgtgc
      361 ggctcgagca ccgcgacggc cgccttcatc acctcggccg actgcagcac gaacgggagc
      421 tgcatctcgc ccgcgccgaa cagctcgccc accgtcttca tgccggccag cagcacctcg
      481 ttgatgatcg cgagggcgcc gcgggtctcg agcgccgcgg ccaggtcggc ctccagcccg
      541 tcgcggaccc cgtcgacgat gcgccgctgg aggcgctcgt cgatcgggag ggcggccagg
      601 tcctcgaccg tggcggactt cgtcgcctcc ttgccctcga acagggccat gaaacgctgc
      661 agcgggtcgt agccctgccg gcggcggtcg tagaccaggt cgagcgcgac ctcgcgctgc
      721 tcctcgtcga tccggtgcat gggcaggatc ttggccggcg acacgatcgc cgcgtccagg
      781 ccggcctccc gcgcctcgtg caggaacacc gagttgagca cctggcgggc ggcgggcgac
      841 agcccgaacg acacgttgga gacgccgagg atcgtccgca ccccgggcag ctcggccttg
      901 atgcgccgga tcgcgtcgag cgtggcgacg gcgtcgcccc gcaggtcctc ctggcccgag
      961 ctgagcggga aggtgagcgc gtcgaaaacc aggtcatcgg gggcgagccc gtgcttgccc
     1021 acggcgaggt cgtggatccg gtgggcgacg cgcagcttcc actcgacgtc gcgggcctgg
     1081 ccctcctcgt cgatcagcag ggcgacgagc gcggcgccgt actgcctggc catcgggcag
     1141 acggcgtcca tgcggcgctc gccgtcctcg aggttgatcg agttgatgat cgcccggccg
     1201 ccgagacgcg cgaggccggc ctcgatcacg tcggcctggg tcgagtcgag catgaccggg
     1261 accgtcgact gcctggcgag ctgctcggcc aggacgtcca tgtcgggcac gccgtcccgc
     1321 cccacgtagt cgacgcagac gtcgaggacg tgcgcgccct cgcggatctg ctccttggcg
     1381 atgccggtca tgccctcgag gtcgccctcg agcatcaggt cgcggaactt gcgggagccg
     1441 ttggcgttgc agcgctcccc cacggtcagg taggtgaggt cctggtcgag cgggaccgac
     1501 tggtagaggc tggacaggct cggctcgtgg accggctcgc ggggcgcggg cgcccggccg
     1561 ccgaccgcct cgacgacctg ccggacgtgc tccggcgtgg tgccgcagca gccgcccacc
     1621 acgttcacgc cgaactcggc cacgaagcgg tcgtgcgcct cggccagctc ggccgggccg
     1681 agcgggtagt gcggggcgcc gtcgcgcagc tcgggcaggc cggcgttcgg caggcaggac
     1741 agcggcgtgc gcgcgtgctt ggagaggtag cgcaggtgct cgcccatctc ggcggggccg
     1801 gtggcgcagt tcatcccgac gacgtcgacg ccgaggggct cgatggcggt gagggcggcc
     1861 gcgatgtcgg acccgagcag catctggccg gtggtctcga ccgtcacctg gaccatcagc
     1921 ggggtcttgc gcccggcccg ccgcatgccg gcgtgagagc cggcgaccgc ggccttgagc
     1981 tggagcaggt cctggcaggt ctcgacgagc agcaggtcgg ccccgccggc gagcagcccg
     2041 tacgcctggt cggcgtaggt ggccagcagc gcgccgtagg tcgtgtgccc gagggtcggc
     2101 agcttcgtgc ccggccccat cgagccggcc acccagcgcg gccggccgtc ggtggcgaag
     2161 tcgtcggcga cctcccgcgc gagccgcgcg gccacct
//
LOCUS       DASPYY010000725         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_681865, whole genome shotgun sequence.
ACCESSION   DASPYY010000725 DASPYY010000000
VERSION     DASPYY010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681865"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VEP73_11380"
     CDS             <1..304
                     /locus_tag="VEP73_11380"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYT27072.1"
                     /translation="WYRCGERPPAGEGSLVGLVSWIVVGLIAGALARFLVPGRDPLGC
                     LGTILLGMAGSLVGGFLANIVLGGRTDLTRSGLIGSVVGAILILLVLRLVRGPRYR"
     gene            488..1057
                     /locus_tag="VEP73_11385"
     CDS             488..1057
                     /locus_tag="VEP73_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT27073.1"
                     /translation="MALRQPGAERPGPRGDDGQARGGGRLRPLELRAWRGFLWVHTAV
                     VRQLDAELRAAHQLSLTSYEVLLYLYQAPGRRLRMSELADSVLLSLSGITRLVDRLTQ
                     AGLVMREPCLDDRRGFYAVLTEEGRARFRAAARTHLAGIREHFLSAFGDEDLEALAEL
                     WARIPLGRDRAGHPSTWDRVTGQPPPHGE"
     gene            1299..>2549
                     /locus_tag="VEP73_11390"
     CDS             1299..>2549
                     /locus_tag="VEP73_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HYT27074.1"
                     /translation="MLQNLPIRAKLFAVLAVPLLVLTALTSVEIRSNVTKGDQADRVN
                     RLAGFAVQLSGFVHDLQRERGLSAGYVAGGAAGDVGMTAQRARVDQDLRDVGTAAAAL
                     DLGSFDPQLGQRLLAAQAQLARLGAERQAVERRSATVGGTLDYYSGAVARLLEVESEV
                     ELETRDSDVLRNASALLALARAKEAASQERDLVNAVLSVGRFAAGQYQRFVTLIGSEE
                     TWTSQFLGSASRSQRALYARTATTGPDVQRAAQLRGDVLESGSGGEITADLSRAAGTA
                     GGKGTAKRNATTWYAAMSARIDLLRQVETSLAGDLAGAAAAARSSASRQAAVSSAILL
                     LALVLTVGISLVTAGSMVGPLRTLRRAAIDVAEHQLPGVVERLQGGEPVDVASGAPPL
                     GIVSRDEIGQVADAFTAVHRVAVQV"
BASE COUNT          296 a          985 c          927 g          341 t
ORIGIN      
        1 ttggtatcgt tgcggggaac gtccgccggc cggggaggga tcgctcgtgg ggctcgtctc
       61 gtggatcgtc gtcgggctca tcgccggggc gctggcccgc ttcctcgtcc ccgggcgcga
      121 cccgctcggc tgcctcggga ccatcctcct cggcatggcg gggtccctgg tcggcggctt
      181 cctcgccaac atcgtgctcg gcggcaggac cgacctcacc cggtccgggc tgatcgggtc
      241 ggtggtcggc gccatcctca tcctgctcgt cctccgcctg gtccgcggcc cccgctaccg
      301 ctgacgccgc tgagcgcgcc tccggggccg cgcgggcggg cgcctgcccc atgccacctt
      361 cgggtacgct ctgtgcgacg cccgtcgccg cggcggccgg gccgagcgcc gcggagagga
      421 gcgccgcctg tcacgaggac cagagcacga cgacgacctc gccccggcgg tcggcgggca
      481 cgccgaggtg gcgctgcggc agccgggcgc cgagcggccc gggccgcggg gcgacgatgg
      541 gcaggcgcgt ggcggcgggc gcctgcgccc gctggagctc cgggcctggc gcgggttcct
      601 gtgggtccac accgccgtcg tccggcagct cgacgccgag ctgcgggccg cccaccagct
      661 ctccctcacc tcctacgagg tcctgctcta cctgtaccag gcgccaggga gacggctccg
      721 catgtcggag ctggccgact cggtcctgct cagcctgagc ggcatcaccc ggctggtcga
      781 ccgcctcacc caggcagggc tggtcatgcg cgagccctgc ctggacgacc gccgcgggtt
      841 ctacgcggtc ctgaccgagg agggccgcgc ccgcttccgc gccgccgccc gcacccatct
      901 ggcgggcatc cgggagcact tcctgtccgc cttcggcgac gaggatctcg aggccctcgc
      961 cgagctgtgg gcgcgcatcc cgctcggccg cgaccgggcc ggccacccgt cgacgtggga
     1021 ccgggtgacc gggcagccgc cgccccacgg cgagtgagcg gccgcccggt gagcccgggt
     1081 caacggcggc gggcgcgccg ggccgcgcag cggctggacc cgaggagttg acggtggtcg
     1141 cggtcgtgct ccaacatgcc gaatcttgac gatccgtcct gctccgcgcg cttgcagctg
     1201 gaagtggtca acaagtagat tctggtcgac tcccccatat gcccaagcac gtctcagggg
     1261 cccggtcgcg tgacgtccgc ggagacgaag ggtggcagat gcttcagaac ctcccgatcc
     1321 gggcgaagct gttcgccgtc ctggccgtcc cgctgctcgt cctcacggcg ctgacctctg
     1381 tcgagatcag gtcgaacgtc actaagggcg accaagccga ccgcgtgaac cggctggcgg
     1441 gcttcgccgt ccagctctcc gggttcgtcc acgacctgca gcgggaacgc ggcctctccg
     1501 ccggctacgt cgccggcggc gccgccggcg acgtcggcat gaccgcccag cgggcgcggg
     1561 tggaccagga cctccgcgac gtcggcaccg ccgccgccgc gctcgacctc ggcagcttcg
     1621 acccgcagct ggggcagcgg ctcctggcgg cccaggccca gctcgccagg ctcggcgcgg
     1681 agcgccaggc ggtcgagcgc cgctcggcga cggtgggcgg caccctcgac tactactccg
     1741 gcgcggtcgc gcgcctgctc gaggtcgagt ccgaggtcga gctcgagacc agggacagcg
     1801 acgtgctgcg caacgcctcg gccctgctcg cgctcgcccg cgccaaggag gccgcgtccc
     1861 aggagcgcga cctcgtcaac gccgtcctgt cggtcggccg cttcgccgcg gggcagtacc
     1921 agcggttcgt gaccctgatc ggctcggagg agacctggac ctcgcagttc ctcggttccg
     1981 cgagccgctc ccagcgtgcc ctctacgcgc gcaccgccac gaccggtccc gacgtccagc
     2041 gggccgcgca gctccgcggg gacgtcctcg agagcgggag cggcggcgag atcaccgcgg
     2101 acctgtcgcg cgccgccggg acggccggcg gcaagggcac cgcgaagcgc aacgccacga
     2161 cctggtacgc cgccatgagc gccaggatcg acctgctgcg gcaggtggag acgagcctcg
     2221 ccggcgacct ggccggcgcg gccgcggcgg ccaggtcatc ggccagccgg caggccgccg
     2281 tgtcgagcgc catcctcctg ctcgccctcg tcctcaccgt gggcatctcg ctcgtcaccg
     2341 ccgggtcgat ggtcggtccg ctgcggacgc tcaggcgcgc cgccatcgac gtggccgagc
     2401 accagctccc cggcgtcgtc gagcgcctcc agggcggcga gccggtcgac gtggcgagcg
     2461 gggcgccccc gctcgggatc gtctcgaggg acgagatcgg ccaggtcgcc gacgccttca
     2521 ccgcggtgca ccgggtcgcc gtccaggtg
//
LOCUS       DASPYY010000726         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2086059, whole genome shotgun sequence.
ACCESSION   DASPYY010000726 DASPYY010000000
VERSION     DASPYY010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086059"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VEP73_11395"
     CDS             complement(<1..347)
                     /locus_tag="VEP73_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT27075.1"
                     /translation="MLLAQVEAFLEVMRLGSVSRAADVLFVSQPTLTARLKNLEAELG
                     EALFVRTGRGLRPTEAGLAFQPHAERAVRALQEGQAVIAELQRGDAGALVVGAAPAAS
                     TYVLPALLRRFVAR"
     gene            518..1918
                     /locus_tag="VEP73_11400"
     CDS             518..1918
                     /locus_tag="VEP73_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HYT27076.1"
                     /translation="MAVPSASYSITMRVHLGADPRGIGQITTAVGEAAGSVVAVDIVE
                     SHPDLLVVDLTCNAADAAHADSITRAVEAVEGAHVHKTSDRTFLLHLGGKLEVTSKVP
                     LRTRDDLSMAYTPGVARVCRAIAENPADARMLTIKRNTVAVVTDGSAVLGLGNIGPAA
                     ALPVMEGKAVLFKQFGGIDAWPVCLDTQDTDEIVRTVELLAPVYGGVNLEDIAAPRCF
                     EVERRLRERLDIPVFHDDQHGTAIVVLAGLLNALRVVDKKLGEIRLVMSGAGAAGVAI
                     IKILQAEGVGDVVACDRGGVLEPGREGLDPSKRWVAEHTNRGGARGSLRDALRGADVF
                     VGVSAPNLLTAGDIATMAPGAVVFALANPDPEVDPAGARRYAAVVATGRSDEPNQINN
                     VLAFPGVFRGALDAGARHITEAMEVAAARAIAARVADDELRPDYIVPSVFDRGVAPAV
                     ARAVQDAVEREQGPQA"
     gene            1915..>2330
                     /locus_tag="VEP73_11405"
     CDS             1915..>2330
                     /locus_tag="VEP73_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYT27077.1"
                     /translation="MTGGGPGPVPGVRAGAVTAWFAGHVPGARPPLEFTPISGGRSNL
                     TYRVTDAGGKAWVLRRPPLSGVLPSAHDMGREHRILTALGPTDVPVPRTYGLCADESV
                     NGAPFYVMEHVDGIVPRDEATVGRWFGPRERGQASRS"
BASE COUNT          313 a          907 c          842 g          268 t
ORIGIN      
        1 cgggccacga agcgccgcag cagcgcgggc agcacgtagg tgctcgccgc cggggcggcg
       61 ccgaccacca gcgcgccggc gtcgccgcgc tgcagctcgg cgatcaccgc ctggccctcc
      121 tgcagtgccc gcaccgcgcg ctccgcgtgg ggctggaagg cgagcccggc ctcggtcggc
      181 ctgaggccgc gcccggtccg cacgaacagc gcctccccca gctccgcctc gaggttcttc
      241 agcctggcgg tgagcgtcgg ctggctgacg aacagcacgt cggcggcccg gctcacgctg
      301 ccgagccgca tgacctcgag gaacgcctca acctgggcaa ggagcaccgg gctcacctcc
      361 cgtccgatcc ggcagcctac ggtaacgcac gggccgccgg gcaaggccct ggggtcgacc
      421 gaggccagag gaccgaggca gcacgaccga ggcaagggga gtagtaggct tcgaccgcgt
      481 cccgcgccgc cggaggcctg gaaggagccc aacgacgatg gcagtcccaa gcgcctcgta
      541 ctcgatcacg atgcgggtgc acctcggcgc cgacccgcgc ggcatcgggc agatcaccac
      601 cgcggtcggg gaggcggccg gctcggtcgt cgccgtggac atcgtcgagt cccaccccga
      661 cctgctggtg gtcgacctga cctgcaacgc cgcggacgcg gcgcacgccg actccatcac
      721 gcgcgcggtc gaggcggtcg agggcgccca cgtccacaag accagcgacc gcaccttcct
      781 cctccacctc ggcggcaagc tcgaggtcac ctccaaggtc cccctgcgga cccgcgacga
      841 cctctccatg gcctacacgc ccggcgtggc ccgcgtctgc cgcgccatcg ccgagaaccc
      901 ggccgatgcg cgcatgctca ccatcaagcg caacacggtc gcggtggtca ccgacggctc
      961 ggcggtcctc gggctcggca acatcggccc ggcggccgcc ctgccggtga tggagggcaa
     1021 ggccgtgctg ttcaagcagt tcggcgggat cgacgcctgg ccggtctgcc tcgacaccca
     1081 ggacaccgac gagatcgtcc gcaccgtgga gctgctcgcg cccgtctacg gcggcgtgaa
     1141 cctcgaggac atcgccgccc cgcgctgctt cgaggtcgag cgccggctgc gggagcggct
     1201 cgacatcccg gtcttccacg acgaccagca cggcaccgcg atcgtcgtgc tggccgggct
     1261 cctgaacgcc ctgcgggtgg tcgacaagaa gctcggcgag atccggctcg tcatgtcggg
     1321 cgccggcgcg gccggggtcg cgatcatcaa gatcctccag gccgagggcg tgggcgacgt
     1381 cgtcgcctgc gaccgcggcg gcgtcctgga gccgggcagg gaggggctcg accccagcaa
     1441 gcggtgggtg gccgagcaca ccaaccgcgg cggcgcgcgg ggcagcctgc gcgacgcgct
     1501 ccgcggcgcc gacgtgttcg tcggcgtgag cgcgcccaac ctgctcaccg ccggcgacat
     1561 cgcgaccatg gcgcccggcg cggtcgtgtt cgccctggcc aaccccgacc cggaggtcga
     1621 cccggccggc gcccggcggt acgcggccgt ggtcgcgacc ggccgcagcg acgagccgaa
     1681 ccagatcaac aacgtgctcg ccttccccgg ggtgttccgc ggcgcgctcg acgccggcgc
     1741 acgccacatc accgaggcga tggaggtggc cgcggcccgc gccatcgccg cccgggtggc
     1801 cgacgacgag ctgcggcccg actacatcgt gccgagcgtc ttcgaccgcg gcgtcgcccc
     1861 cgccgtggcc cgggcggtcc aggacgccgt cgagcgggag cagggcccgc aggcatgacg
     1921 ggcggcggcc ccggcccggt tcccggcgtc cgcgccgggg ccgtcacggc ctggttcgcc
     1981 gggcacgtcc ccggggcgcg cccgccgctc gagttcaccc cgatcagcgg cgggcgctcg
     2041 aacctcacct accgggtcac cgacgccggg gggaaggcgt gggtgctgcg gcggccgccg
     2101 ctctccggcg tcctgccgtc cgcccacgac atggggcggg agcaccggat cctcaccgcg
     2161 ctcggcccca ccgacgtgcc ggtcccccgc acctacgggc tgtgcgccga cgagtcggtc
     2221 aacggcgcgc cgttctacgt catggagcac gtcgacggca tcgtccccag ggacgaggcc
     2281 accgtggggc gctggttcgg gccgcgcgag cgcgggcagg cgagccggtc
//
LOCUS       DASPYY010000727         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_821864, whole genome shotgun sequence.
ACCESSION   DASPYY010000727 DASPYY010000000
VERSION     DASPYY010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821864"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VEP73_11410"
     CDS             <1..905
                     /locus_tag="VEP73_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HYT27078.1"
                     /translation="VDEVAQCLMELGDAQALDAWAACTNRPDNTRGSVFGTAETLLDV
                     FGDPTVAASAEGCDVDLDALLTAPNSTLYLYAPASEQERLRALFELLVSVVIARAEEL
                     AAQRPDGMLSPRLFVCLDEAGNCAAIRKLPQLATTGRGQGIQLLTIWHDEAQLADRYG
                     RRAATVLNGHRAKLFLSGQADPASLELASKLIGEEAISQTSQTTGDGRQSQTESVAWR
                     RLVPVEALRQLKPRRAVLVYGHLPPVRIRLRPWFRSPRLRALANAAVPTARVPRRAVP
                     APAAGVEPVAPVPAAAGEDEGVAA"
     gene            902..>1530
                     /locus_tag="VEP73_11415"
     CDS             902..>1530
                     /locus_tag="VEP73_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27079.1"
                     /translation="MSATPTAPTSGPGDRPGDPRPAFERVAYAWLDREVDHGQPLDPI
                     GLACEVSVSARVAAGLLAVLRAQRARDPGLGELRARRVRDRITEVFVTRELPGGPPLD
                     PATLAGEVGTTTTTARQWLHTLRTAHGSDPRLAGVRTEPVSHGAPTAEQLAALQAAYT
                     RGGRPQVEPAGPKRPLERIEQLYRDREVTRGQRLDPGEVACEVGVGRSYV"
BASE COUNT          186 a          616 c          548 g          180 t
ORIGIN      
        1 acgtcgacga ggtcgcccag tgcctgatgg agctgggcga cgcccaggcc ctggacgcct
       61 gggcggcctg caccaaccgg cccgacaaca cccgcgggag cgtcttcggc accgccgaga
      121 cgctgctgga cgtcttcggc gaccccaccg tcgccgcctc ggcggagggc tgcgacgtcg
      181 acctggacgc gctgctcacc gcgccgaaca gcacgctgta cctgtacgcc cccgcgtccg
      241 agcaggagcg cctgcgggca ttgttcgagc tgctcgtcag cgtggtgatc gcccgcgcgg
      301 aggagctcgc cgcccagcgc ccggacggga tgctgtcgcc ccggctgttc gtctgcctgg
      361 acgaggcggg caactgcgcc gcgatccgca agctccccca gctcgccacc accggccgag
      421 ggcaaggcat ccagctgctg accatctggc acgacgaggc gcagctggcc gaccgctacg
      481 gccgccgcgc cgccacggtc ctcaacggcc accgcgccaa gctgttcctg tccgggcagg
      541 ccgaccccgc ctccctcgag ctcgcaagca agctgatcgg cgaggaggcc atcagccaga
      601 cctcccagac caccggggac ggccgccagt cacaaaccga gtccgtggcc tggcggcggc
      661 tggtgccggt ggaggcgctg cggcagctca agccccgccg ggcggtgctc gtctacgggc
      721 acctcccgcc ggtgcgcatc cggctgcgcc cgtggttccg ctccccgcgg ctgcgcgcgc
      781 tcgcgaacgc agccgtgccc accgcgcggg tgccccgtcg tgccgtgccg gccccggcgg
      841 cgggtgtcga gccggtggcg cccgtgcctg cggcggctgg ggaggacgag ggggtggccg
      901 cgtgagcgcc accccaaccg ccccgacgtc agggcctggg gaccggccgg gtgacccgcg
      961 ccccgccttc gagcgggtcg cctacgcctg gctggaccgc gaggtcgacc acggccagcc
     1021 cctggacccc attggcctcg cttgtgaggt cagcgtcagc gctcgggtcg ccgccgggct
     1081 gctggcggtg ctgcgcgctc agcgcgcccg cgaccccggc ctgggggagc tgcgcgcccg
     1141 gcgggtccgc gaccgcatca ccgaggtgtt cgtcacccgc gagttgcccg gcggcccgcc
     1201 gcttgacccg gccaccctcg ccggcgaggt tggcaccacg accaccaccg cccggcagtg
     1261 gctgcacacc ctgcgcaccg cccacgggtc cgacccgcgc ctggcgggcg tgcgcaccga
     1321 gccggtcagc cacggcgccc cgaccgccga gcagctcgcc gcgttgcagg ccgcctacac
     1381 ccgtggcggc cgcccccagg tcgagccagc cgggccgaag cggccgctgg agcgcatcga
     1441 gcagctctac cgcgaccgcg aggttacccg cggccagcgc ctggacccgg gcgaggtcgc
     1501 gtgtgaggtc ggcgtcggcc gcagctatgt
//
LOCUS       DASPYY010000728         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_260056, whole genome shotgun sequence.
ACCESSION   DASPYY010000728 DASPYY010000000
VERSION     DASPYY010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260056"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /gene="hisD"
                     /locus_tag="VEP73_11420"
     CDS             complement(<1..1365)
                     /gene="hisD"
                     /locus_tag="VEP73_11420"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYT27080.1"
                     /translation="MLPLIDLRGSDRDPSGLLPRAPAADVAAARASVRALVGDVAARG
                     DAAVAEAARRFDRVDAPPGRWRATAEELARAGRALDPGLRDALRDATERVRAFHEVQR
                     PAPAVFAGRPGVRLVQRFEPLRRAGVYVPGGLGAYPSSVVMNVVPAQAAGVGQVALAT
                     PPGPGGRGHPAVLGAAALLGIDEVWLLGGAQAVAALALGTATVPATDSVTGPGNLYVA
                     LAKREVAGRVRTDAFAGPTEIAVLADATADPAHVAADLVAQAEHDPLAACLLVTDDPA
                     VWAAVEPVLAREVAATAHRDRVRRALAGQSAVVLCDDRAAMLRVAEAFAPEHLELLVA
                     DARELASLVRGAGAVFVGAWTPVSLGDYAAGTNHVLPTAGTARFSAGLSTLDFLRPVQ
                     VAEFDRDALAAAAPTVAALALAEDLPAHARAVEVRLARPGPAGDRDTRPAPEGARSQS
                     EGASL"
     gene            1583..>1778
                     /locus_tag="VEP73_11425"
     CDS             1583..>1778
                     /locus_tag="VEP73_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYT27081.1"
                     /translation="MELPLFPLSTVLYPGLPLPLHVFEERYRQLFARVLAGDRRFGVV
                     AIVEGREVGGESTYQPVGCLA"
BASE COUNT          196 a          660 c          753 g          169 t
ORIGIN      
        1 gagactggcg ccctcggact gggaccgcgc gccctcgggc gcgggccggg tgtcccggtc
       61 gccggccggg ccggggcggg cgaggcggac ctcgacggcc cgggcgtggg cgggcaggtc
      121 ctcggccagg gccagggcgg ccacggtcgg cgcggcggcg gcgagcgcgt cgcggtcgaa
      181 ctccgccacc tgcaccgggc ggaggaagtc gagggtggac aggccggccg agaaccgggc
      241 cgtcccggcc gtcggcagga cgtggttggt gcctgccgcg tagtccccca gcgacaccgg
      301 cgtccacgcg ccgacgaaca ccgcgcccgc cccgcgcacc agcgaggcca gctcgcgggc
      361 gtccgccacg agcagctcga ggtgctcggg ggcgaacgcc tcggcgaccc ggagcatggc
      421 ggcccggtcg tcgcagagca cgaccgccga ctggccggcc agcgcgcggc ggacccggtc
      481 ccggtgggcg gtcgcggcga cctcgcgcgc gagcaccggc tcgaccgccg cccagacggc
      541 ggggtcgtcg gtgacgagca ggcaggcggc cagcgggtcg tgctccgcct gggcgaccag
      601 gtcggcggcg acgtgggcgg ggtcggcggt cgcgtcggcc agcacggcga tctcggtggg
      661 gccggcgaag gcgtcggtgc gcacccggcc cgcgacctcc cgcttggcca gggccacgta
      721 gaggttgccc ggcccggtga ccgagtcggt ggccggcacg gtggcggtgc cgagcgcgag
      781 cgcggcgacg gcctgggcgc cgccgagcag ccagacctcg tcgatcccga gcagcgcggc
      841 ggcacccagg acggcggggt ggccccggcc gcccggcccg ggcggggtgg cgagcgcgac
      901 ctggccgacc ccggcggcct gggcgggcac cacgttcatg acgacgctgg acgggtaggc
      961 gccgagcccg ccgggcacgt acacgccggc ccgccggagc ggctcgaagc gctggaccag
     1021 gcggacgccg gggcgcccgg cgaagaccgc cggggcggga cgctgcacct cgtggaacgc
     1081 gcgcacccgc tcggtcgcgt cgcgcagggc gtcgcggagc ccggggtcga gggcgcgccc
     1141 ggcgcgggcg agctcctcgg cggtggcccg ccagcgcccg ggcggtgcgt cgacccggtc
     1201 gaaccggcgc gccgcctcgg ccaccgccgc gtcgccgcgg gcggccacgt ccccgacgag
     1261 cgcgcggacg ctcgcccggg cggcggccac gtcggcggct ggcgcacgcg gcagcaggcc
     1321 ggacgggtcg cggtccgagc ctcgcaggtc gatcaggggc agcaacggtc ggctccaggc
     1381 aggtgggcca cgcgttcgcg ggcgggaggt gacgcggcgg cgcgtctgtc ggcgggaccc
     1441 caaggtacct cagcgccgcc ggtcgggcga agccggtttc cacaggtgcg gcaagcgccg
     1501 cgcgacggcc cggggacctg ccactacgat gggcgggagg tcaggcaggg acgaggccgg
     1561 gagaggtcgg gaggaggtcg ccgtggagct tccgctcttc ccgctgtcca ccgtgctgta
     1621 cccgggcctg ccgctgccgc tccacgtgtt cgaggagcgc taccggcagc tgttcgcccg
     1681 cgtcctcgcc ggcgaccgcc gcttcggcgt ggtcgcgatc gtcgagggcc gcgaggtcgg
     1741 cggggagtcg acctaccagc cggtcggctg cctggccg
//
LOCUS       DASPYY010000729         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1103820, whole genome shotgun sequence.
ACCESSION   DASPYY010000729 DASPYY010000000
VERSION     DASPYY010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103820"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /gene="pafA"
                     /locus_tag="VEP73_11430"
     CDS             complement(<1..1062)
                     /gene="pafA"
                     /locus_tag="VEP73_11430"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HYT27082.1"
                     /translation="MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVEELITHDKAGERILESLLAAAEARLHEEGI
                     AGQIFLFKNNTDSAGNSYGCHENYLVSRYGEFQRLADVLIPFFVSRQILCGAGKVLAT
                     PRGAVYCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEW
                     SAFLKVGATDLVLSMVEEATVLRDLTLENPIRAIREISHDMTCRRKVRLATGREASAL
                     EIQREYFDKAVRFVDRRDDPVAKKVVSMWGHVLDALEAQELDRIGRQVDWVTKYLLIE
                     RARARHGIPLSHPKVALMDL"
     gene            complement(1175..>2282)
                     /locus_tag="VEP73_11435"
     CDS             complement(1175..>2282)
                     /locus_tag="VEP73_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15"
                     /protein_id="HYT27083.1"
                     /translation="VTVPAGDPGALAAAAGDRAWLAAGTVPGRTPVEREAAARALLDL
                     RLLLRPGGAAIAAQPPYWAYVWPRDASFAAAAFHATGHDAEGRAILGFLARTQRRDGT
                     WPARSRPDGSTVDDGRPPQLDATGWVCWAAFLVTGQAADRAPVRRVWPMVRAAADAAA
                     RSLGTDGLPPAGPDYWERAEHAPTLGTAAALLAGLHAAARLAPAAGDPAAGARWERAG
                     ERLGRAVRRRFAGPGGYRHSPYGGGPDAAVAWLAPPFQRPDPAVRAAVAAAARQLAPG
                     GGGGVVPGSPWRGGDPWTPATAALALAETAGGDHAAGDRRLGWLLAHRTTLGAFPERV
                     RRGDGAPRSVAPIGWTTAIVLLALTARDHPLPTP"
BASE COUNT          268 a          846 c          895 g          273 t
ORIGIN      
        1 gaggtccatc agcgcgacct tggggtggga cagcggtatc ccgtgcctgg cccgcgcccg
       61 ctcgatgagc aggtacttgg tcacccagtc gacctggcgg ccgatgcggt cgagctcctg
      121 cgcctcgagg gcgtcgagca cgtggcccca catggacacg accttcttgg cgaccgggtc
      181 gtcgcggcgg tcgacgaacc tgaccgcctt gtcgaagtac tcccgctgga tctcgagggc
      241 gctcgcctcc cggccggtgg ccaggcggac cttgcgccgg caggtcatgt cgtgggagat
      301 ctcccggatg gcgcggatcg ggttctcgag ggtcaggtcg cgcaggaccg tcgcctcctc
      361 gaccatcgag agcacgaggt cggtcgcgcc caccttgaga aaggccgacc actccgacat
      421 gttggagtcg cccacgatga cgtgcaggcg gcggtagcgc tcggcgtcgg cgtggggttc
      481 gtccctggtg ttgatgatcg gccgcgagcg ggtcgtcgcc gacgacacgc cctcccagat
      541 gtgctcggcc cgctgcgcga tgcagtacac cgcgccgcgc ggggtggcga gcaccttgcc
      601 ggccccgcac aggatctggc gggacacgaa gaacgggatc agcacgtcgg ccaggcgctg
      661 gaactccccg tagcgggaca cgaggtagtt ctcgtggcag ccgtaggagt tgccggccga
      721 gtcggtgttg ttcttgaaca ggaagatctg gccggcgatg ccctcctcgt gcaggcgggc
      781 ctcggccgcc gcgagcaggc tctcgaggat ccgctccccc gccttgtcgt gggtgatcag
      841 ctcctcgacc gagtcgcact ccggcgtggc gtactccggg tgggagccca cgtcgaggta
      901 gagccgggcg ccgttctcga ggaacacgtt cgacgaccgt ccccacgaga ccacccgccg
      961 gaacaggtag cgggcgacct cgtcgggcga gagccggcgc tggcctcgga acgtgcaggt
     1021 gaccccgtac tcgttctcga ggccgaaaat ccgccgatcc atgggctcat gctaccggtc
     1081 cccgccgtgt cgtggggcgc tcgacccgcc gccgtcacct gtgccgggcc gcctcctggt
     1141 cggcccgtcc ttcccgcacc agtgcgggcg cgggtcaggg cgtgggcagc gggtggtcgc
     1201 gggcggtcag ggcgagcagg acgatcgcgg tcgtccagcc gatgggcgcg accgaccggg
     1261 gcgcgccgtc gccgcgccgg acccgctccg ggaacgcgcc cagggtggtg cggtgggcca
     1321 gcagccagcc gagccggcgg tccccggccg cgtggtcgcc gcccgctgtc tccgccaggg
     1381 cgagcgcggc ggtggccggg gtccacgggt ccccgccacg ccagggcgag cccggcacga
     1441 ccccgccgcc cccgccgggg gcgagctgcc gcgcggccgc ggccaccgcg gcccgcaccg
     1501 ccgggtccgg ccgctggaac ggcggcgcca gccaggccac ggcggcgtcc ggtccgcccc
     1561 cgtacggcga gtgccggtag ccgcccggcc cggcgaaccg gcggcggacg gcgcggccga
     1621 gccgctcgcc ggcccgttcc cagcgggccc cggcggccgg gtcgccggct gccggggcga
     1681 gcctggcagc ggcgtggagg ccggcgagca gcgcggccgc cgtcccgagg gtcggggcgt
     1741 gctcggcccg ctcccagtag tcggggccgg cgggaggcag gccgtcggtg ccgagcgagc
     1801 gggccgccgc gtcggcggcc gcccgcacca tcggccacac gcgccgcacc ggcgcccggt
     1861 cggccgcctg gccggtgacc aggaacgccg cccagcacac ccagccggtc gcgtcgagct
     1921 ggggcggccg accgtcgtcg accgtggagc cgtcagggcg cgagcgggcc ggccaggtgc
     1981 cgtcgcgccg ctgcgtgcgg gcgaggaacc cgaggattgc ccggccctcg gcgtcgtggc
     2041 cggtggcgtg gaacgcggcg gccgcgaagc tcgcgtcccg tggccacacg taggcccagt
     2101 agggaggctg ggcggcgatc gccgccccgc cgggacggag caggagccgc aggtcgagga
     2161 gcgcgcgcgc ggcggcctcg cgctcgaccg gggtccggcc cgggaccgtc ccggcggcga
     2221 gccaggcgcg gtcgccggcc gccgcggcca gggcgcccgg gtcgccggcg ggcaccgtga
     2281 cc
//
LOCUS       DASPYY010000730         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2790324, whole genome shotgun sequence.
ACCESSION   DASPYY010000730 DASPYY010000000
VERSION     DASPYY010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790324"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="purH"
                     /locus_tag="VEP73_11440"
     CDS             <1..1324
                     /gene="purH"
                     /locus_tag="VEP73_11440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYT27084.1"
                     /translation="ADRRRPEHVDQLEAAGIAPFDLVVSNLYPFEATAADPEAGEELA
                     VERIDVGGPAMVRAAAKNFASVAVVCDPADYGLVESALAAGGTTLDERRALAARAFAR
                     TAAYDGAVAAWFARDETFPATLPLAGRLVRTLRYGENPHQGAALYALGGHGSGLAGAV
                     QVAGKELSYNNLLDADAALGLAAEFPGRPFAVVVKHTNPCGAAVGPTLEAAYGAALEG
                     DPTSAYGGVVGLSRPLDEATARRIAGVFTEVVVAPGFEALAREVLAGKPSLRLVEAAL
                     ATPPGARGARPAPSLAVRSVAGGLLVQDADLVPDDPASWTVAAGTPPDPATADDLALA
                     WTVARHVKSNAIVLVGRGRVVGVGAGQMSRVDAVRIAVAKAGERARGAVAGSDAFFPF
                     PDGLEALAAAGVRAVAQPGGSVRDAEVTAAAERGGLTLLLTGRRHFRH"
     gene            1347..>2169
                     /locus_tag="VEP73_11445"
     CDS             1347..>2169
                     /locus_tag="VEP73_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HYT27085.1"
                     /translation="MPARILDGRAVGAQVRAELAEEIEQLAAAGRRPGLAAVLVGDDE
                     ASHIYVGAKQRAAASTGLDSRRVALPADATRSQVLDAVAELNADPAVHGIIVQLPLPG
                     GIDPVEVQEAIDPAKDVDGLHPWNEGRLLRGDPAFAPCTPAGIVELLRRERVPVEGSH
                     VVIVGRGLLVGRPLAVMLSAKAPGANATVTLCHTGTRKLSGYTREADVLVAAAGRPGM
                     VVADMVKPGAAVVDVGNHRLDGALVGDVAPEVAEVAGALTPVPGGVGPMTVAMLLA"
BASE COUNT          246 a          835 c          840 g          248 t
ORIGIN      
        1 cgccgaccgc cgccgcccgg agcacgtcga ccagctggag gcagccggga tcgcgccctt
       61 cgacctggtc gtctcgaacc tctacccctt cgaggcgacg gcggccgacc ccgaggcggg
      121 cgaggagctg gccgtcgagc ggatcgacgt cggcggcccg gccatggtgc gggcggcggc
      181 caagaacttc gcgtcggtgg cggtcgtctg cgaccccgcc gactacggcc tggtggagtc
      241 cgccctggcc gccggcggga ccactctgga cgagcgccgc gccctggccg cgcgggcgtt
      301 cgcgcggacg gccgcctacg acggcgcggt ggcggcctgg ttcgcccgcg acgagacgtt
      361 cccggcgacg ctgccgctgg ccggccggct cgtgcgtacg ctccgctacg gcgagaaccc
      421 gcaccagggg gccgccctct acgccctggg cggccacggg tcggggctcg ccggcgccgt
      481 ccaggtcgcc ggcaaggagc tctcctacaa caacctgctc gacgccgacg ccgccctcgg
      541 cctggccgcc gagttcccgg gccggccctt cgcggtggtg gtcaagcaca ccaacccgtg
      601 cggcgccgcg gtcggcccga ccctggaggc ggcctacggg gccgcgctgg agggcgaccc
      661 gacgagcgcc tacggcggcg tcgtcggcct gtcccggccg ctcgacgagg cgaccgcacg
      721 gcgcatcgcg ggcgtgttca ccgaggtcgt ggtcgccccg gggttcgaag ccctggcgcg
      781 ggaggtgctg gcaggcaagc cgtcgctccg gctcgtcgag gcggccctgg ccacgccgcc
      841 cggagcgcgc ggggcacggc cggccccgtc cctggccgtg cgctcggtgg ccggcggcct
      901 gctcgtgcag gacgccgacc tcgtccccga cgacccggcc agctggacgg tggccgcggg
      961 caccccgccc gacccggcga ccgcggacga cctcgccctc gcctggacgg tcgcgaggca
     1021 cgtgaagtcg aacgcgatcg tgctggtcgg ccgcgggcgg gtcgtcggcg tcggggcggg
     1081 ccagatgagc cgggtcgacg ccgtccggat cgcggtcgcg aaggccggcg agcgggccag
     1141 gggcgcggtc gccggcagcg acgccttctt cccgttcccg gacgggctgg aggcgctggc
     1201 cgccgccggg gtccgggccg tggcccagcc cggcggcagc gtccgggacg ccgaggtgac
     1261 cgccgcggcc gagcggggcg ggctgaccct gctgctcacc ggccgccgcc acttccgcca
     1321 ctagctctgg agcacgagga gaggatatgc ccgcaaggat cctcgacggt cgcgcggtcg
     1381 gcgcccaggt ccgggccgag ctggccgagg agatcgagca gctcgcggcg gccgggcgcc
     1441 gaccaggcct ggccgcggtc ctggtcggcg acgacgaggc gtcccacatc tacgtgggcg
     1501 ccaagcagcg ggcggccgcc agtacgggcc tcgactccag gcgggtcgcc ctgcccgccg
     1561 acgcgaccag gagccaggtg ctcgacgcgg tcgccgagct gaacgccgac cccgccgtgc
     1621 acggcatcat cgtccagctg cccctgcccg gcggcatcga cccggtggag gtccaggagg
     1681 ccatcgatcc ggccaaggac gtcgacggcc tgcacccgtg gaacgagggc cggctcctgc
     1741 ggggcgaccc cgcgttcgcg ccgtgcaccc cggccgggat cgtcgagctg ctgcgtcggg
     1801 agcgggtgcc ggtcgagggc agccacgtcg tcatcgtcgg ccgcggcctg ctggtgggcc
     1861 gcccgctcgc cgtcatgctc tccgccaagg cgcccggagc gaacgcgacc gtcaccctct
     1921 gccacaccgg cacccgcaag ctctccggct acaccaggga ggccgacgtg ctggtcgccg
     1981 cggccgggcg gcccggcatg gtggtcgcgg acatggtcaa gccaggagcc gccgtggtcg
     2041 acgtcggcaa ccaccggctg gacggtgccc tggtcggcga cgtggccccc gaggtggccg
     2101 aggtggccgg cgcgctcacc ccggtgccgg gcggcgtcgg cccgatgacg gtcgcgatgc
     2161 tgctcgcca
//
LOCUS       DASPYY010000731         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1104889, whole genome shotgun sequence.
ACCESSION   DASPYY010000731 DASPYY010000000
VERSION     DASPYY010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104889"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEP73_11450"
     CDS             complement(<1..601)
                     /locus_tag="VEP73_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT27086.1"
                     /translation="MLAERGDELAALIATELGMPLTLSKMIQAGLPTMSFGSMAEQIE
                     KIAWEEEVGNSLIVREPIGVVGAITPWNFPFMLNLSKITPALAAGNSVVLKPAPDTPW
                     SATFIGRLAAEQTDIPPGVLNVVTSGDAADERRAPRRVGRGLEDDGVARRERGRDLRQ
                     VQHEREVPRRDRADHAERLAQGVGECIFLYRDGVTCDFAA"
     gene            572..1633
                     /locus_tag="VEP73_11455"
                     /pseudo
     CDS             572..1633
                     /locus_tag="VEP73_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            1705..2532
                     /locus_tag="VEP73_11460"
     CDS             1705..2532
                     /locus_tag="VEP73_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HYT27087.1"
                     /translation="MCPFRASAGLTERWDHFGYGERDGVATVTFDRPERLNALTFDTY
                     ADLRDLLAELPHRDGVRVLVLTGAGRGFCSGGDVHEIIGALRGMGPRELLDFTRMTGA
                     VVQRMRELPLPVVAAVNGVAAGAGSVLALAADFRVLARSASLVFLFTRVGLAGADMGA
                     AYLLPRLVGLARATELLLLGDRVDAEQAATLGLANQVVDDAELGAAAGALARRLAEGP
                     ALAYAATKALLSRELDMSLAGAVELEAVTQALLMTSEDHGEFLAAFTEGRAPRWTGR"
     gene            2529..2981
                     /locus_tag="VEP73_11465"
     CDS             2529..2981
                     /locus_tag="VEP73_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYT27088.1"
                     /translation="MSAPPPGAGGTPHLVVNPAGLAPPVGFAHAVVAAPGRTVHLGGQ
                     AAQRADGSIQGATVVEQFDVAAGNVVAALAAAGGRPEHLVALQIYVTDAAAYRAALAQ
                     LGAVWRRHFGRHYPATALLEVAGLLDPAALVELVATAVVPDVPCPTAP"
BASE COUNT          377 a         1211 c         1181 g          410 t
ORIGIN      
        1 gagcggcaaa gtcacaggtg acaccatccc ggtacaggaa aatgcattca cctacaccct
       61 gcgcgagccg ctcggcgtgg tcggcgcgat cacgccgtgg aacttcccgt tcatgctgaa
      121 cctgtcgaag atcacgcccg cgctcgcggc gggcaacacc gtcgtcctca agcccgcgcc
      181 cgacacgccg tggagcgcga cgttcatcgg ccgcgtcgcc cgacgtcacg acgttcagca
      241 cgcccggcgg gatgtcggtc tgctcggccg cgaggcgccc gatgaacgtc gcgctccacg
      301 gcgtgtcagg agcaggcttg agcactaccg agttgccggc tgcgagcgcg ggcgtgatct
      361 tcgacaggtt gagcatgaac gggaagttcc acggtgtgat cgcgccgacg acgccgatgg
      421 gctcgcggac gatcagcgag ttgccgacct cctcctccca ggcgatcttc tcgatctgct
      481 cggccatcga gccgaagctc atggtcggca ggccggcctg gatcatcttc gacagcgtca
      541 gcggcatgcc cagctcggtg gcgatgagcg ccgcgagctc gtcgccgcgc tcggcgagca
      601 cggcctcctc ccccgcctgt tcccccgctc cgcgggcggc acggccgacc acgccatgtc
      661 agcgctcgac ctgtgcctgc accgggaggc gctcgcgcag gagtcgaccg aggcggagac
      721 cgcgctcgcc ctccagggac tcggggccca cccgatcctg tcggcgggat ccgaggcgct
      781 cgtgcgccgc cacgtccccg aggtcgcggc cgggcgggcg ctggccgcgt tcgccctgtc
      841 ggaggagggc gccggctcgg acgcggcagg gctcgagctc gccgccgagc gggacgggcg
      901 cggcttccgg ctgacaggcg ccaagcagtg gatctccaac gcgcccgacg ccgacgtcta
      961 caccgtgttc gcccgcacca ccccgggcgc ccgggcgcgc gggatcaccg cgttcgccgt
     1021 gcccgccggc gcgccaggcc tcggtggcga gccgatcgag ctgctcagcc cccacccgat
     1081 cgggcggctc gagttcgacg gcgtgcccgt cggcgccggt caggtcctcg gccaggtcga
     1141 cggcggcttc ggggtcgcca tgcgcaccct cgacctgttc cggccgagcg tcggcgcgtc
     1201 cgcggtgggg atggcgcagg cagccctcga cctggccgtc gcccacgcgg cgaccaggcg
     1261 cgcgttcggc gcgcccctgc gcagcttcca ggccgtgtcg caccggctcg ccgagatggc
     1321 cacccggacc gaggcggccc gcctgctcgt ctacgcagcg gccagccgct acgacgcggg
     1381 cggcggcccg cgcccggccg gcggggccga cggggtgacc agggcgtccg ccatggccaa
     1441 gctgtacgcg accgagaccg cccaggccgt ggtcgacgcg gccgtccagg tccacggggc
     1501 gtcggcgctg gtgcgcgggc accctctcga gcacctctac cgggacgtgc gcgcggcccg
     1561 catctatgag ggcacctcgg agatccagcg ggccgtgatc gcccgggagc tgttccaggc
     1621 gggcccgggc tgagccgggc cgggacggcc gccgccaggt gggctaccgt tcgttcctga
     1681 cgaccaggga ggagggagag cgccgtgtgc ccgttccgtg cgtcggccgg cctgaccgag
     1741 cgctgggacc acttcggcta cggggagcgc gacggtgtcg ccacggtcac gttcgaccgg
     1801 ccggagcggc tgaacgcgct caccttcgac acctacgccg acctgcgcga cctgctcgcc
     1861 gagctgcccc accgggacgg cgtgcgggtg ctggtgctca cgggggccgg ccgggggttc
     1921 tgctcgggcg gcgacgtgca cgagatcatc ggcgccctgc ggggcatggg accgcgggag
     1981 ctgctcgact tcacgcgcat gaccggggcg gtggtgcagc gcatgcgcga gctgccgctg
     2041 ccggtggtcg cggccgtcaa cggggtcgcc gccggggccg gctcggtgct cgcgctcgcg
     2101 gccgacttcc gcgtcctcgc ccggtcggcg tcgctcgtgt tcctgttcac cagggtcggg
     2161 ctggccggtg ccgacatggg cgccgcctat ctcctgcccc gcctggtcgg gctggcgcgg
     2221 gcgaccgagc tgctgctgct cggcgaccgg gtggacgccg agcaggcggc caccctcggg
     2281 ctcgccaacc aggtcgtcga cgacgccgag ctgggcgcgg cggccggcgc cctggcccgg
     2341 cgcctggcgg aggggccggc cctcgcctac gccgcgacga aggcgctgct ctcgcgggag
     2401 ctcgacatga gcctggccgg tgcggtcgag ctcgaggcgg tcacccaggc gctgctgatg
     2461 acctccgagg accacggcga gttcctcgcc gccttcaccg aggggcgggc gccgcggtgg
     2521 acgggccggt gagcgcgccg ccgcccggag cgggcggcac gccccacctc gtcgtgaacc
     2581 cggcggggct cgccccaccg gtcggcttcg cccacgcggt ggtggcggca cctggccgca
     2641 ccgtccacct tggcggccag gccgcccagc gggcggacgg gtcgatccag ggggccaccg
     2701 tcgtcgagca gttcgacgtc gccgccggca acgtcgtggc ggccctggcc gccgccggcg
     2761 gccggcccga gcacctggtg gcgctgcaga tctacgtcac cgacgccgcc gcgtaccggg
     2821 ccgccctggc ccagctgggc gccgtctggc ggcgccactt cggccgccac taccccgcca
     2881 ccgccctgct cgaggtcgcc ggcctgctgg acccggcggc gctggtcgag ctggtcgcca
     2941 ccgccgtggt ccccgacgtg ccgtgcccca cggcaccctg agcaccgggg cctggggccg
     3001 gcccttggcg ccggcctggg gccggccctt atggaatact cgggagcgac cggccagcgc
     3061 tcgcgacagg accgcaccat gaccgagacg tcgaccgaga cggcgaccga gacggcggcg
     3121 accgcgctcg acttcctcga gatcgaccgg cagctgtcgg aggaggagcg cctcatccg
//
LOCUS       DASPYY010000732         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1949981, whole genome shotgun sequence.
ACCESSION   DASPYY010000732 DASPYY010000000
VERSION     DASPYY010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949981"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VEP73_11470"
     CDS             <1..221
                     /locus_tag="VEP73_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27089.1"
                     /translation="HGLPTVRLPTPAWAPARSATEARAVAAVGGDLRPLLPATVARAV
                     RRTGAYDEDPAGYLARARELDALAADGA"
     gene            480..1346
                     /locus_tag="VEP73_11475"
     CDS             480..1346
                     /locus_tag="VEP73_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HYT27090.1"
                     /translation="MDHTTFLHRPALRRPVLVAAFEGWNDAGEAASTALGFVAATLDA
                     ETFAQIDPEEFYDFQQTRPTVRLADGDSRVIEWPSVQFQAVRLPDANRDLILVRGNEP
                     NLRWRTFAREVLDVARGLDAEMLVTLGALLADVPHTRPIQVVGSAGDPALADKLGLAT
                     SRYEGPTGILGVLADVAHSAGVPAVSLWAALPHYVQLTPNPRAALALVERLGALLSLS
                     LETSTLGEATETFDATVADVVAEDPELAGYVERLEADHDQDASSLENLPPDQFVAEVE
                     RYLRDHPGGSRP"
     gene            complement(1440..1649)
                     /locus_tag="VEP73_11480"
     CDS             complement(1440..1649)
                     /locus_tag="VEP73_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYT27091.1"
                     /translation="MTTGVVKWFNPDKGYGFIARPDGDDVFVHFSAIQAEGYRSLDEG
                     QMVEFEVTQGPKGLQAANVRALPPQ"
BASE COUNT          295 a          782 c          678 g          266 t
ORIGIN      
        1 agcacgggct gccgacggtc aggctgccga ccccggcctg ggccccggcc cgctcggcga
       61 ccgaggcccg cgccgtggcg gccgtcggcg gcgacctccg cccgctgctc ccggcgacgg
      121 tcgcgcgcgc ggtccggcgg accggcgcct acgacgagga cccggccggc tacctggccc
      181 gcgcccggga gctggatgcg ctggcagccg acggcgcgtg agccaggtgc ggcacggccg
      241 tccgggagcc gagccggtgc tggcgcccgt cgggacgtga aggacccccg cgccgtccgg
      301 gggggccaag acagcgcgag ggcctcggtt gtaatagtac tgcttcgacg caagttttgt
      361 agtcctccgg gcggacgaca tgggatcgtc aagttcgtgt gggttgcagt acagagcaaa
      421 cctcggcacc gctgcgtagc ccgataccac gggacgccga acgatggcat cgtggagaga
      481 tggaccacac caccttcctg caccggcccg ccctgcgccg cccggtcctg gtcgccgcct
      541 tcgaaggatg gaacgacgcc ggcgaggcgg ccagcaccgc cctcggcttc gtggccgcca
      601 ccctcgacgc ggagaccttc gcccagatcg accccgagga gttctacgac ttccagcaga
      661 cgaggccgac ggtccggctc gccgacggcg acagccgcgt gatcgagtgg ccgtcggtcc
      721 agttccaggc cgtccgcctg cccgacgcca accgcgacct catcctggtc cgcggcaacg
      781 agcccaacct gcgctggcgg accttcgccc gggaggtgct cgacgtcgcc agggggctcg
      841 acgcggagat gctggtcacc ctcggcgccc tgctcgccga cgtgccccac acccggccga
      901 tccaggtggt cggctcggcc ggggacccgg cgctggccga caagctcggc ctggccacgt
      961 cccgctacga gggcccgacc ggcatcctcg gggtgctcgc cgacgtcgcc cactccgccg
     1021 gcgtcccggc ggtcagcctc tgggcggcgc tcccccacta cgtgcagctc acgcccaacc
     1081 cgcgggccgc cctcgccctg gtcgagcggc tcggcgcgct gctctccctg tcgctcgaga
     1141 cgagcacgct gggcgaggcg accgagacgt tcgacgcgac cgtggccgac gtcgtcgccg
     1201 aggacccgga gctcgccggc tacgtcgagc ggctggaggc cgaccacgac caggacgcgt
     1261 cctcgctcga gaacctcccg cccgaccagt tcgtcgccga ggtcgagcgc tacctgcgcg
     1321 accacccggg cggcagccgg ccctgaggcg gccccgggcg ccgccgccgt gctgtgatcc
     1381 agccgaacgg cccgccgcgt tgaacgcggc gggccgttcg cccctcagcc cacgtcagct
     1441 cactgcggcg gcaacgctcg cacgttggcc gcctggagac ccttgggtcc ctgcgtcacc
     1501 tcgaactcca ccatctgccc ttcgtcgagc gagcggtacc cctccgcctg gatcgcggag
     1561 aagtgcacga acacgtcgtc gccgtccgga cgagcgatga agccgtaccc cttgtcaggg
     1621 ttgaaccact tgacgacccc agtggtcacg aaacctgttc cccccttcca tccttgccgt
     1681 tcaacttgag gccgggcccc acacggaccg gcgggcaggg acagctcgaa ccgcactgcg
     1741 gaaagcgtgc accgctgctc gcgcggaact ctagcaccag gtcagtcatg tcaatagagt
     1801 caatttgtgc aaaccagcct cagtactcca gcgtattcgc gcggaagccg gcgaactggc
     1861 gcgggtggag gcatgatgac cgtacgatcg agcgcccgac cgacgccgag caccaggagg
     1921 cagccccatg gagccagtcc ggccgtccac cggctggggc gtcacccacc tctactaccg
     1981 ggtcgacccg ggccgctgcg ccgaccccgg cctcgccggc a
//
LOCUS       DASPYY010000733         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1387544, whole genome shotgun sequence.
ACCESSION   DASPYY010000733 DASPYY010000000
VERSION     DASPYY010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387544"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VEP73_11485"
     CDS             <1..467
                     /locus_tag="VEP73_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYT27092.1"
                     /translation="QMQLDQAAKDMSGAVDALLAMPETTGPGVGVVGFCVGGGLALYL
                     ATLKPEVAAVVDYYGFPREGLDWDLSAVKGIVLGHYAEDDDFASRELADGIEEELRDA
                     GVRVTFYHYPGTQHAFFNDSRPEVHDPEAADLSWRRTLDFLHGALGGAPRPS"
     gene            682..2043
                     /locus_tag="VEP73_11490"
     CDS             682..2043
                     /locus_tag="VEP73_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HYT27093.1"
                     /translation="MATRPGTTTRTERTAGRGRRIPLALAAVATIPGSLLGAAALLRP
                     GGAELAPPLAATLYGLAVIGAAFLLGWAAEAAQLDVSASLAIGALALVAVLPEYAVSF
                     VFAWKGGNDVERFGAACKPPGGGESNCSLVLANMTGANRLLIGIGWALVVFVAWWRWR
                     RRGEPREGITLGRSKSVELSFLTLACAYSLTLPLKRTITLLDAVVLVAIFVAYTLRIS
                     KAPAEEPHLIGPAAWVGGLSRRARRTAVVALFSFAAAVILLVAEHFAEALVDTGTELG
                     ISEFFLVQWLAPLASEAPELLVAGLYAWQLKTGEALTTLVSSKVNQWTLLVGTLPLVF
                     AVASSSLHGLPIEAAQREELLLTAAQTVFAVAVLVNLSMSLKEAGAIFALFWAQFIIG
                     ALVPPSLHGVELVGLSAVYLLLGVALLVRHRSDFRTRLREGFRTPYDRLGEPAAAREG
                     PPA"
     gene            complement(2065..3294)
                     /locus_tag="VEP73_11495"
     CDS             complement(2065..3294)
                     /locus_tag="VEP73_11495"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HYT27094.1"
                     /translation="MLHFGAAVSTVPDHLEALDEAVQAATAGLGGTPDVAICFFSMEH
                     AAMASGIALSLSERLGTSAIVGCTAEGVIGGGRELEGGPGLALWVARLPGVDVRPFAL
                     EVVPVPDGYGIAGWPSLAPEDPATVVLLADPFSFPADPFLERLNEEQPGLPVVGGMAS
                     GGVAPGEHRLLSGTDALDRGAVGVALVGPVDVRTVVSQGCRPIGSPYAVTRAEGNVVH
                     ELGGQPALERLRQVVASLSLREQELIRGGIQVGQVIDEHKAEFGRGDFLIRGLTGADE
                     DSGAIAVGDTVELGQTLQFHIRDATAAGEELELLLGPVAGWNPRGVLLFSCNGRGRRF
                     FGEPDHDARRVAAATGQAPMAGFFAQGELGPVGGRNFLHGYTASMAVFCEPLEQVPAV
                     ELARAAESTGAGAPEPV"
     gene            complement(3355..4530)
                     /locus_tag="VEP73_11500"
     CDS             complement(3355..4530)
                     /locus_tag="VEP73_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYT27095.1"
                     /translation="MRARQRRAVVAIALLAAVSTATARLVAVHSDEVAAAAADPVRGR
                     FASVVSDPSSFKGELLVWASGGFSTAEVKRVRDSVQVAAIAAVRTGLLAVESNSPSRG
                     PGYRAVPVEAMAANREAYAAAVGRPAGQLSAMLRKGAVLSQTSARLRKLRSGGRLRLV
                     GGRTLRVTGVVPDWVVGGYEVAVDKDQGRQLHLNRVTYLLLRPRGPRPAFEAAIRRLL
                     PRRALRFQTREDRPFLRPGDGVLPLAQVKLRFGEFAVRSLARPDPDPRWVRANIVRGV
                     LPVLGPVRCHRLVVANLAAAMGDLQRMRLERLVDVADFRRQGGCWQPRPPWAGGPELS
                     RHLWGIAVGLDVAANPSGARPVADPRLVRVMASHGFTWGGRWLRPEGGQFEWVGSGA"
     gene            complement(4636..5949)
                     /locus_tag="VEP73_11505"
     CDS             complement(4636..5949)
                     /locus_tag="VEP73_11505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYT27096.1"
                     /translation="MPAFQLPDLGEGLTEAEIVTWHVQTGDTVTVDQPIVEVETAKAV
                     VEIPAPYAGTVTKLHAAEGETLEVGAPLLSVDTGGAPAARDGSATPAEEPAGPDRQAT
                     LVGPGERQQGRRRRAAAPAARLPGARRGGPAHVPPGPAGHAGRPKATPPVRKYAKDRG
                     VDLALLAGTGKDGRVTREDVDRALAPDAAPERPSAAPAGARAPRDHAEERIPVRGTRK
                     QIAAAMVASASSIPHVTEFLTVDATALMELRARLRALPAAAGAKLTPLAVIAKALCVA
                     VRQFPLMNSSWDDAAGEIVVKGWVNLGIATDTPTGLLVPNVKDADLLGILDLSSELAR
                     VTGLARERKAAPPDLQGGTITITNVGGFGVETGTPIINRPECAILATGLIAPRPWVVD
                     GELAVRQLMTASVSFDHRIVDGAYAARFLAHLRDLLEDPALLAAF"
     gene            complement(5950..>6863)
                     /locus_tag="VEP73_11510"
     CDS             complement(5950..>6863)
                     /locus_tag="VEP73_11510"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYT27097.1"
                     /translation="GPLGGVFRVTDGLAARFGDTRVFDTPLAESGIVGTAIGMAMNGL
                     RPVVEMQFDAFAYPAFEQITSHLAKLRNRTRGRVALPVVIRIPYGGGIGGVEHHCDSS
                     EVYYTHTAGLRVVTPATPGDAYALLRQAIECPDPVIFLEPKRRYWSKQATVLDHAGAT
                     LDRAIVRRPGSDVTLITYGGTLGTVLEAADAAEEEGRSVEVIDLRSLSPFDDETVTAS
                     VRRTGRAIVVHEASRFCGYGAEVAARLSEQCFHYLEAPILRVAGFDIPYPPPHLEEYH
                     LPSVDRILDAVDRLQWQETRPAVKAGG"
BASE COUNT          868 a         2552 c         2538 g          905 t
ORIGIN      
        1 tgcagatgca gctcgaccag gccgccaagg acatgagcgg cgcggtcgac gcgctcctcg
       61 cgatgcccga gacgacgggc ccgggcgtcg gcgtggtcgg cttctgcgtc ggcggcggcc
      121 tcgcgctcta cctcgcgacg ctgaagccgg aggtcgccgc ggtcgtcgac tactacgggt
      181 tcccgcgcga gggcctcgac tgggatctct cggccgtgaa gggcatcgtg ctcggccact
      241 acgccgagga cgacgacttc gccagccgcg agctggccga cgggatcgag gaggagctcc
      301 gcgacgcggg cgtgcgcgtg accttctacc actacccggg cacccagcac gccttcttca
      361 acgacagccg gcccgaggtc cacgaccccg aggcggccga cctgtcctgg cggcgcaccc
      421 tggacttcct ccacggcgcg ctcggcggcg ccccccggcc cagctgaccc gcacacgggt
      481 gccgggcacc cgtgtgcgcc gcgttgcggg agcgggttag gatgggggcg aaggggagta
      541 gtcctcacga ggcttcctgc ctcatgagca cgccgtcatc acggtgggcg ccgagcccgc
      601 ccggcgcctc acccgccctg cgggggacga gaccttcgag ccgttcgaca tcgtgttcga
      661 tgccagcgac ccggaggttc catggccacg agaccaggga cgaccacccg caccgagcgc
      721 acggcaggac gcggccggcg catcccgctc gcgctcgccg ccgtcgcgac catccctgga
      781 agcctcctcg gcgccgccgc gctgctgcgc cccggcggcg ccgagctcgc tcccccgctg
      841 gccgcgacgc tgtacgggct cgccgtgatc ggcgccgcct tcctgctcgg ctgggcggcc
      901 gaagctgccc agctggacgt ctcggccagc ctggccatcg gcgcgctcgc actcgtcgcc
      961 gtcctgcccg agtacgcggt cagcttcgtc ttcgcctgga agggcgggaa cgacgtggag
     1021 cgcttcggtg cggcctgcaa gccgcccggc ggcggcgagt ccaactgctc gctggtgctc
     1081 gcgaacatga ccggcgcgaa ccggctcttg atcggcatcg gctgggcgct cgtcgtgttc
     1141 gtcgcctggt ggcgctggcg gcggcgcggc gagccgcgcg aggggatcac cctcggccgc
     1201 tccaagtcgg tcgagctgtc gtttctgacc ctggcctgcg cgtactcgct caccctgccc
     1261 ctgaagcgga cgatcacgct gctcgacgcc gtcgtcctcg tcgccatctt cgtcgcctac
     1321 acgctgcgca tctccaaggc cccggccgag gagccgcacc tgatcggccc cgccgcctgg
     1381 gtcggcggcc tctcccgccg ggccaggcgg accgccgtgg tcgcgctgtt ctcgttcgcc
     1441 gccgccgtca tcctgctcgt ggccgagcac ttcgccgagg cgctggtcga cacggggacc
     1501 gagctgggca tcagcgagtt cttcctcgtc cagtggctcg ctccgctcgc ctcggaggcg
     1561 cccgagctgc tggtggccgg gctctacgcg tggcagctga agaccgggga ggcgctcacc
     1621 accctggtct cctccaaggt caaccagtgg acgctgctcg tcggcaccct gccgctggtg
     1681 ttcgcggtcg cgtcgtccag cctgcacggc ctgcccatcg aggccgccca gcgcgaggag
     1741 ctgctcctga ccgcggcgca gaccgtcttc gcggtcgcgg tcctcgtgaa cctcagcatg
     1801 tcgctgaagg aggcgggcgc catcttcgcg ctgttctggg cgcagttcat catcggcgcg
     1861 ctcgtgccgc cctcactcca cggcgtcgag ctggtcgggc tctcagcggt ctacctgctg
     1921 ctcggcgtcg ccctgctcgt ccgtcaccgc agcgacttcc ggacgcggct ccgcgagggc
     1981 ttccgcaccc cctacgaccg gctcggggag ccggccgccg ccagggaggg cccgccggcc
     2041 tagccagccg ggcagccgtg cgcgtcagac cggctcgggg gcgccggccc cggtcgactc
     2101 ggccgcgcgg gcgagctcga cggccgggac ctgctcgagc ggctcgcaga acaccgccat
     2161 gctggcggtg tagccgtgca ggaagttgcg cccgccgacc ggccccagct cgccctgcgc
     2221 gaagaagccg gccatcggcg cctggccggt ggccgccgcg acccgcctgg cgtcgtggtc
     2281 gggctcgccg aagaaccggc ggccccggcc gttgcacgag aacagcagga cgccccgcgg
     2341 gttccagccc gccaccggtc cgagcaacag ctcgagctcc tcgccggcgg cggtcgcgtc
     2401 cctgatgtgg aactgcagcg tctggcccag ctccacggtg tcgcccaccg cgatcgcgcc
     2461 gctgtcctcg tcggccccgg tgagcccgcg gatcaggaag tcgccccggc cgaactcggc
     2521 cttgtgctcg tcgatcacct gcccgacctg gatcccgccg cggatgagct cctgctcgcg
     2581 caggctgagg gaggccacga cctggcggag ccgctccagc gcgggctgcc cgcccagctc
     2641 gtggacgacg ttgccctcgg cgcgggtgac cgcgtagggc gacccgatcg gccggcagcc
     2701 ctggctcacc accgtgcgca cgtcgacggg gccgacaagg gccacgccga ccgcaccgcg
     2761 gtcgagcgcg tcggtgcccg agagcagccg gtgctcgccg ggtgcgaccc cgccggacgc
     2821 catcccgccg accaccggca ggccgggctg ctcctcgttc agccgctcga ggaacgggtc
     2881 ggccgggaac gagaacgggt ccgccaggag caccacggtc gccgggtcct cgggagcaag
     2941 gctgggccag ccggcgatgc cgtagccgtc cggcaccggc acgacctcga gggcgaacgg
     3001 gcgcacgtcg acgcctggca gccgcgccac ccagagggcg aggccgggac cgccctccag
     3061 ctcgcggccg cctccgatca cgccctcggc cgtgcagccg acgatcgccg aggtgccgag
     3121 gcgctcggag aggctgagcg cgatcccgga ggccatcgcg gcatgctcca tcgagaagaa
     3181 gcagatggcg acgtcgggcg tgccgccgag gccggcggtg gcggcctgga cggcctcgtc
     3241 gagggcctcg aggtggtcgg ggacggtcga gacggcggcg ccgaagtgca gcatgggcac
     3301 gtcgagtcct caggttgcgg ccatggacgg caagcctacc cctcatggcg cgggtcaggc
     3361 gcccgagccc acccactcga actggccgcc ttccgggcgc agccagcgcc cgccccaggt
     3421 gaagccgtgg ctggccatca cgcggacgag ccgcgggtcg gccaccggcc tggccccgga
     3481 cgggttggcc gcgacgtcga ggccgaccgc gatgccccag aggtgcctgg acagctcggg
     3541 gccgcctgcc cacggagggc gcggttgcca gcagccgccc tggcgccgga agtcggcgac
     3601 gtcgaccagc cgctcgaggc gcatgcgctg caggtcgccc attgccgcgg ccaggttcgc
     3661 gacgaccagg cggtggcagc gcaccgggcc gagcacgggc agcacgcccc gcacgatgtt
     3721 ggcgcgcacc cagcggggat cggggtcggg ccgggcgagg ctgcgcaccg cgaactcgcc
     3781 gaagcggagc ttgacctggg cgagcggcag cacgccgtcg cccgggcgga ggaacggccg
     3841 gtcctcccgg gtctggaagc gcagcgcccg gcgcgggagg aggcggcgga tcgccgcctc
     3901 gaacgccggc cggggcccgc gcgggcgcag cagcaggtag gtgacgcggt tcaggtggag
     3961 ctgccggccc tggtccttgt cgacggcgac ctcgtagccg ccgacgaccc agtcggggac
     4021 gacgccggtg acgcgcaggg tcctgccgcc gacgagcctg agccgccccc cggaacggag
     4081 cttgcgcagc cgggcgctgg tctgcgagag cacggcgccc ttgcgcagca tcgccgagag
     4141 ctggcccgcc ggccggccga cggcggcggc gtacgcctcg cggttggcgg ccatcgcctc
     4201 gaccggcacg gcccggtagc cgggcccgcg gctcgggctg ttgctctcca cggcgagcag
     4261 cccggtccgg accgcggcga tcgccgccac ctggaccgag tcgcggaccc gcttcacctc
     4321 ggccgtcgag aagcccccgg aggcccagac cagcagctcg cccttgaagc tcgagggatc
     4381 ggagacgacg ctcgcgaacc tgcccctgac cgggtcggcc gcggcggcgg cgacctcgtc
     4441 ggagtggacg gcgacgagcc gcgccgtcgc ggtggagacg gccgcgagca gggcgatcgc
     4501 gaccaccgct cgccgctgcc tggctcgcat acgaccctcc ttcgccgcct tctcaagggt
     4561 aaagggacga cgcaaccgtg agcagtgccg gccgtgcccg ccgcgtcgag acccgggccg
     4621 cgcggggccg gcgcgtcaga acgccgcgag gagggccggg tcctcgagca ggtcgcgcag
     4681 gtgggccagg aaccgggcgg cgtaggcgcc gtcgacgatc cggtggtcga acgacaccga
     4741 ggcggtcatg agctggcgca cggccagctc gccgtccacc acccacggcc gcggcgcgat
     4801 caggccggtg gccaggatgg cgcactcggg gcggttgatg atcggcgtgc cggtctcgac
     4861 cccgaacccg cccacgttgg tgatcgtgat ggtcccgccc tgcaggtccg gcggcgcggc
     4921 cttgcgctcg cgggcgaggc cggtcacccg cgcgagctcg ctcgacaggt cgaggatgcc
     4981 gagcaggtcg gcgtccttga cgttcggcac cagcaggccg gtcggcgtgt cggtggcgat
     5041 cccgaggttg acccagccct tgacgacgat ctccccggcc gcgtcgtccc aggacgagtt
     5101 catgaggggg aactggcgca cggccacgca gagcgccttc gcgatgaccg cgagcggcgt
     5161 cagcttggcg ccggcggcgg cgggcagcgc cctgaggcgg gcgcggagct ccatcagggc
     5221 ggtcgcgtcg acggtgagga actcggtgac gtgcgggatc gaggacgccg aggcgaccat
     5281 ggcggccgcg atctgcttgc gggtgccccg gaccgggatg cgctcctcgg cgtggtcgcg
     5341 cggcgcgcgg gcgccggcgg gggcggccga cggccgctcg ggggccgcgt cgggcgccag
     5401 cgcccggtcg acgtcctccc tggtcacgcg gccgtccttg ccggtgccgg cgagcagcgc
     5461 gaggtcgacg ccccggtcct tggcgtactt ccgcacgggc ggggtcgcct tgggacggcc
     5521 cgcgtgcccg gccgggccgg gcgggacgtg tgccggtccg ccccggcggg ctcctgggag
     5581 cctcgccgcc ggggcggccg cgcggcgccg ccgcccttgc tggcgctcgc ccggaccgac
     5641 gagcgtcgcc tgccggtccg gcccggccgg ctcctcggcg ggtgtggccg agccgtccct
     5701 agccgcgggc gcgccaccgg tgtcgacgct caggagcggc gcgccgacct ccagggtctc
     5761 gccttccgct gcgtgcagct tggtgaccgt gccggcgtac ggggccggga tctcgacgac
     5821 cgccttggcc gtctcgacct cgacgatggg ttggtccacg gtaacggtgt ctccggtctg
     5881 cacgtgccag gtcacgatct cggcctcggt caggccctcg cccagatccg gaagctggaa
     5941 cgctggcatc tagccgcctg ccttcacggc cgggcgggtc tcctgccact gcaggcggtc
     6001 gaccgcgtcg aggatgcggt cgacgctggg caggtgatat tcctccaggt gaggtggggg
     6061 ataggggatg tcgaacccgg ccacccgcag gatcggggcc tcgaggtagt ggaagcactg
     6121 ctcggacagg cgcgcggcta cctcggcgcc gtatccgcag aaacggctcg cttcatgcac
     6181 cacgatggca cggccggttc gccgcaccga tgcggtcacc gtctcatcgt cgaacgggga
     6241 caggctccgc aggtcgataa cctcgacgct gcggccctct tcctccgcgg cgtcggccgc
     6301 ttccaacacg gtgcccaggg tcccgccgta cgtgatcagg gtcacgtcgg agcccggccg
     6361 ccgcacgatg gcacggtcca gggtggcgcc ggcgtggtca aggaccgtgg cctgcttcga
     6421 ccagtagcgg cgcttgggtt cgaggaagat aaccgggtct gggcattcga tcgcctgccg
     6481 gagaagcgca tacgcgtccc cgggcgtggc cggggtgacg acccgcaggc cggcggtatg
     6541 cgtgtagtag acctcggagg agtcgcagtg gtgttccacg cccccgatgc cgccgccgta
     6601 gggaatccgg atcacgacgg gcagcgcgac gcggcccctg gtccggttcc gcagcttggc
     6661 cagatggctg gtgatctgct cgaaggctgg ataggcgaac gcgtcgaact gcatctccac
     6721 aacgggccgc aggccgttca tcgccatgcc gatggcagtg ccgacgatgc ctgactcggc
     6781 gaggggggtg tcgaagaccc gcgtgtcccc gaacctggcc gccagcccgt cggtgacccg
     6841 gaaaacgcct cccagcggcc cta
//
LOCUS       DASPYY010000734         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1950662, whole genome shotgun sequence.
ACCESSION   DASPYY010000734 DASPYY010000000
VERSION     DASPYY010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950662"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VEP73_11515"
     CDS             complement(<1..1087)
                     /locus_tag="VEP73_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT27098.1"
                     /translation="MAGQAGSVQGGDTASTRRGRAPSYVHGARAVPLLGETIGQNLDR
                     TAARFGDRDAVVSVHQGVRLTYAQFLEAVEEVARGLMTLGIEAGDRVGIWSPNNAEWV
                     TLQYATAKAGAILVNINPAYRTAELAYALAQSRVSTLVLAAAFRQADYVAMVDEAAPE
                     APALSRRVVLGADAPPGAISWDDLRERAAGTTVDALRAREALLQFDDPINIQYTSGTT
                     GFPKGATLSHHNILNNGFFIGEGCGYSEADRVCIPVPLYHCFGMVLGNLACTTHGAAM
                     VYPAEAFDPVATLAAVEAERCTSLYGVPTMFIAELEHPRFGEFRLDTLRTGIMAGSPC
                     PVEVMKKVQADMHMAEVTICYGMTETSP"
     gene            1263..2563
                     /locus_tag="VEP73_11520"
                     /pseudo
     CDS             1263..2563
                     /locus_tag="VEP73_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028829.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
BASE COUNT          328 a          945 c          934 g          356 t
ORIGIN      
        1 ccgggctcgt ctcggtcatc ccgtagcaga tcgtgacctc ggccatgtgc atgtcggcct
       61 ggaccttctt catcacctcg accgggcacg gggagccggc catgatgccg gtgcgcagcg
      121 tgtccagccg gaactcgccg aaccgggggt gctcgagctc ggcgatgaac atcgtcggca
      181 ccccgtagag gctggtgcag cgctcggcct cgacggccgc cagggtcgcg accgggtcga
      241 acgcctcggc cgggtagacc atggccgcgc cgtgggtggt gcaggcgagg ttgccgagga
      301 ccatgccgaa gcagtggtac agcggcaccg ggatgcagac ccggtcggcc tcgctgtagc
      361 cgcagccctc gccgatgaag aagccgttgt tgaggatgtt gtggtgcgac agggtggcgc
      421 ccttcgggaa gcctgtggtc ccggacgtgt actggatgtt gatcgggtcg tcgaactgca
      481 gcagcgcctc gcgggcgcgc agggcgtcga ccgtcgtccc ggcggcccgc tcccggaggt
      541 cgtcccagct gatcgcgccc ggcggggcgt cggccccgag cacgacgcgg cgggacaggg
      601 ccggggcctc gggggctgcc tcgtcgacca tggcgacgta gtccgcctgc cggaacgcgg
      661 ccgccagcac gagcgtggac acgcgcgact gggcgagcgc gtaggcgagc tcggccgtcc
      721 ggtacgccgg gttgatgttc accaggatcg cgcccgcctt cgcggtggcg tactgcaggg
      781 tcacccactc ggcgttgttg ggcgaccaga tgccgacccg gtcgccggcc tcgatgccga
      841 gggtcatgag ccctcgcgcg acctcctcga cggcctcgag gaactgcgcg taggtgagac
      901 ggacgccctg gtgaacgctg acgacggcgt cccggtctcc gaagcgggcc gccgtccggt
      961 ccaggttctg cccgatggtc tcgccgagca gcggcaccgc gcgcgccccg tgcacgtacg
     1021 agggcgcccg cccgcgccgg gtcgacgcgg tgtctccgcc ctggaccgag cctgcctgtc
     1081 ctgccatggc cgcctcctga tcgtccgacc gctccccgat gctcgaccca gggggcatcc
     1141 cgccgcaaga cccggacggc caggcaccgc ccctccgtac ccggttgccg gaaccgcggc
     1201 gaaccgcgac gatgtgcgcc atgacctcct cactgcgcgc cgggcccgag gcgtggacgc
     1261 ccgggtcgtg gcgcctgcgg gacgccgcgc agcagccccg gtggcccgac ccggatgccc
     1321 tggagctggt gctcggccgg gtggcgaggc tgccgccgct ggtgttcgcc ggggaggtgc
     1381 gcagcctgca ggggctgctc gcgcgggtcg cggccggcca cgggttcctg ctccacgccg
     1441 gggactgtgc cgagtcgttc gacgcgttct cggccgactc gatccgcgac aagctcaagg
     1501 tcatcttgca gatggccgtc gtgctcacct acggcgccgg cctgccggtg gtgaagctcg
     1561 gccgcatcgc cggccagttc gccaagcccc gcagcgcgcc gaccgagctg gtcgacggcg
     1621 tcgagctgcc cagctaccgc ggggacatgg tcaacggcgc ggacccgacc ctcgaggcgc
     1681 gccgcgccga cccggcgcgg ctgctcgacg cctaccacca gtcgtcggcc accctgaacc
     1741 tgctgcgcgc gttcaccaag ggcggcttcg ccgacctgca ccaggtccac gtctggaacc
     1801 aggagttcgt cgcccgcagc ccggccggcc agcgctacga ggcggccgcc gacggcatcg
     1861 accgggcgct gcggttcatg gccgcctgcg gcgtcgacct gaccgcctcc tcggtgctcc
     1921 accaggtcga cttcttcacc tcccacgagg cgctcctgct cggcttcgag gaggccatga
     1981 cgcgccgcga cagcctcacc ggcggctggt acgacacctc ggcgcacctg ctgtggctgg
     2041 gggaccggac ccgccagcgc gacggcgccc acgtcgagtt cctgcgcggg gtcggcaacc
     2101 cggtcggggt caagctcgga ccctcggcga cccccgggga agcggtcgcc ctgtgcgagg
     2161 cgctggaccc gcaccgggtg ccgggccggc tggtgctgat cagcaggctg ggggccggac
     2221 gggtgcgcga gctgctccca cccctggtcc gtgcggtggc ggcgagcggc cacccggtcg
     2281 tctgggcctg cgacccgatg cacggcaaca ccttcgtggg gccgtcgggg cacaagacgc
     2341 gccggttcga cgacgtcatg gccgagctgc ggggcttctt cgaggtccac cggaccgggg
     2401 gcagccaccc gggcggcatc cacgtcgagc tgaccggcga ggacgtcacg gagtgcctcg
     2461 gcggtgccga ggagatcctc gacggccatc tcggccagcg ctacgagacc gcctgcgacc
     2521 cacgcctgaa cgccagccag gccctcgagt gcgccttcca ggt
//
LOCUS       DASPYY010000735         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_825528, whole genome shotgun sequence.
ACCESSION   DASPYY010000735 DASPYY010000000
VERSION     DASPYY010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825528"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1969
                     /gene="dnaK"
                     /locus_tag="VEP73_11525"
     CDS             71..1969
                     /gene="dnaK"
                     /locus_tag="VEP73_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYT27099.1"
                     /translation="MAKPVGIDLGTTNSVIATLEGGEPTVIPNAEGSRTTPSVVAFTD
                     TGERLVGQLARRQAILNPKGTVYSVKRFMGRRYDEATEEAKAVTFDVVPGPNGEARIK
                     VRDKLYAPEEISAMILRKLADDAGKFLGERVNQAVITVPAYFNDAQRQATKDAGRIAG
                     LEVLRIINEPTAAAMAYGLDKREHETVLVFDLGGGTFDVSILDVGDGVVEVRATAGDT
                     HLGGDDFDRRLVDYLADQFKKDNGIDLRNDPQALQRLFEAAEKAKVELSSVTQTQVNL
                     PFVTADASGPKHLTATIMRSTFEQITSDLLERCLGPVRQAMSDAKVTANDLDEVILVG
                     GATRMPAVQGLVRRLTGGKEPNMTVNPDEVVALGAAIQAGVLTGEGPDVLLLDVTPLS
                     LGLETLGGVMTKVLERNTTIPTRRSEVFSTAEDNQPAVDVVVLQGERERAADNRVLGR
                     FRLENIRPAPRGEPQVEVTFDVDANGILNVSARDKDTGAQQAITISESSNLDRSEVER
                     MVTDAERNRSEDVRLRELVDARNELDSVAYQVERTLRDLGDAVPQHERARAEMLVADA
                     RQAIKEEAPLDRMRSLTAELQQLYHGLLARAGGAGAQGGAGGAPGGPGGGQPGGGDDV
                     IDADFTQS"
     gene            1978..2457
                     /locus_tag="VEP73_11530"
     CDS             1978..2457
                     /locus_tag="VEP73_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYT27100.1"
                     /translation="MTTQQPDPEAAAREEAAAVAELEDRWRRALADLDNLRKRYAREL
                     GRERAAERARVAAAWLPVIDNLELALAHGDADPLAVLEGVQAVRDQALAVLARLGYPR
                     QDEVGVPFDPARHEAVTVIDDEPEAAPGTVLRVLRPGYGQGEEQLRPAAVAVTGWRG"
     gene            2464..>2754
                     /locus_tag="VEP73_11535"
     CDS             2464..>2754
                     /locus_tag="VEP73_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYT27101.1"
                     /translation="MARDYYEVLGVPRTATAEEIQQAYRKRARTSHPDVNGDPGAEER
                     FKEINEAYQVLKDPDTRARYDRFGPDFRQIPEGVDPAGAGAGGFRGARAGGSP"
BASE COUNT          445 a          962 c         1042 g          305 t
ORIGIN      
        1 tcatagcctg attgacacgg cgtaacgggg ttaccaggca gcggacagcg acgacagaca
       61 ggaggccgcg atggcgaagc cggtgggcat cgaccttggc acgaccaact ccgtgatcgc
      121 aacgctggag ggcggcgagc cgacggtcat cccgaacgcc gagggctcgc gcacgacccc
      181 gtcggtggtc gccttcaccg acaccggcga gcgcctggtc ggccagctgg cccggcggca
      241 ggcgatcctc aaccccaagg gcaccgtcta ctcggtgaag cggttcatgg gccgccgcta
      301 cgatgaggcg accgaggagg cgaaggcggt caccttcgac gtcgtccccg gcccgaacgg
      361 cgaggcccgc atcaaggtgc gggacaagct ctacgcgccc gaagagatca gcgcgatgat
      421 cctgcgcaag ctcgccgacg acgccgggaa gttcctcggc gagcgggtga accaggcggt
      481 catcaccgtg cccgcctatt tcaacgacgc gcagcggcag gcgaccaagg acgccgggcg
      541 gatcgccggg ctcgaggtgc tgcgcatcat caacgagccg accgcggccg cgatggccta
      601 cgggctcgac aagcgcgagc acgagaccgt gctggtgttc gacctgggcg gcggcacctt
      661 cgacgtgagc atcctcgacg tcggcgacgg cgtggtcgag gtgcgggcga ccgccggcga
      721 cacccacctg ggcggcgacg acttcgaccg ccggctggtg gactacctgg ccgaccagtt
      781 caagaaggac aacgggatcg acctccgcaa cgacccgcag gcgctgcagc ggctgttcga
      841 ggcggccgag aaggccaagg tcgagctcag ctcggtgacc cagacgcagg tcaacctccc
      901 gttcgtcacc gcggacgcct cggggcccaa gcacctgacc gccacgatca tgcgctcgac
      961 gttcgagcag atcacctccg acctgctgga gcggtgcctc ggaccggtgc ggcaggcgat
     1021 gtccgacgcc aaggtgaccg ccaacgacct cgacgaggtc atcctggtgg gcggcgcgac
     1081 ccgcatgccg gccgtgcagg ggctggtccg gcggctgacc ggcggcaagg aacccaacat
     1141 gaccgtcaac cccgacgagg tcgtggccct gggcgcggcc atccaggccg gggtgctcac
     1201 cggcgagggg ccggacgtgc tgctgctcga cgtcaccccg ctgtcgctgg ggctcgagac
     1261 gctcggcggg gtcatgacca aggtgctcga gcgcaacact acgatcccga cccggcgcag
     1321 cgaggtcttc tccacggcag aggacaacca gccagcggtc gacgtggtgg tgctgcaggg
     1381 cgagcgcgag cgggcagccg acaaccgggt gctcgggcgc ttccggctgg agaacatccg
     1441 cccggcgccc cggggcgagc cgcaggtcga ggtcaccttc gacgtcgacg ccaacggcat
     1501 cctcaacgtc tccgcccgcg acaaggacac cggcgcccag caggcgatca cgatcagcga
     1561 gagcagcaac ctcgaccggt ccgaggtcga gcgcatggtc accgacgccg agcgcaaccg
     1621 gtccgaggac gtgcgcctgc gcgagctggt ggacgcccgc aacgagctgg actcggtcgc
     1681 ctaccaggtc gagcgcaccc tgcgcgacct cggcgacgcg gtgccgcagc atgagcgggc
     1741 ccgggcggag atgctggtcg ccgacgcgcg ccaggccatc aaggaggagg cgccgctgga
     1801 ccgcatgcgg tcgctgaccg ccgagctgca gcagctctac cacggcttgc tggcccgggc
     1861 cgggggagca ggcgcgcagg gcggcgcggg cggagccccg ggcggcccgg gtggcgggca
     1921 acccgggggc ggcgacgacg tgatcgacgc cgacttcacc cagagctgag gccgacggtg
     1981 acgacccagc agccagaccc ggaggcggcg gcgcgggagg aggcggcggc cgtggccgag
     2041 ctggaggacc gctggcggcg ggccctggcc gacctcgaca acctgcgcaa gcgctacgcg
     2101 cgggagctgg ggcgcgagcg ggcggccgag cgtgcccggg tggcggccgc gtggctgccg
     2161 gtgatcgaca acctcgagct cgcgctcgcg cacggggacg ccgacccgct cgcggtcctc
     2221 gagggggtgc aggcagtgcg cgaccaggcg ctggcggtgc tggcccgtct cggctacccg
     2281 cgccaggacg aggtcggcgt gcccttcgac ccggccaggc acgaggccgt caccgtgatc
     2341 gacgacgagc ccgaggcggc cccgggcacg gtgctgcggg tgctgcgccc cggctacggc
     2401 cagggcgaag agcagctgcg gccggcggcg gtggcggtca ccggctggcg ggggtgagcc
     2461 ggagtggcgc gtgactacta cgaggtgctg ggggttccgc ggacggcgac cgccgaggag
     2521 atccagcagg cctaccggaa gcgggcgcgc acctcccacc cggacgtcaa cggggaccca
     2581 ggggccgagg agcggttcaa ggagatcaac gaggcctacc aggtgctcaa ggacccggac
     2641 acccgggcac gctacgaccg cttcggcccg gacttccgcc agatccccga gggcgtcgac
     2701 ccggccggcg cgggcgcggg tggcttccgc ggtgcccgtg ccgggggctc gccg
//
LOCUS       DASPYY010000736         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2229715, whole genome shotgun sequence.
ACCESSION   DASPYY010000736 DASPYY010000000
VERSION     DASPYY010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229715"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VEP73_11540"
     CDS             complement(<1..614)
                     /locus_tag="VEP73_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT27102.1"
                     /translation="MAELAVRGGAPVRPQGYPAWPVHDERDVEAVASVVRGGRWGGFP
                     EPGPLAAEFAERFAAYQGSRHGVVMSNGTVTMEVALKALGIGWGDEVIVPALTFSATP
                     YAAMAAGALPVFCDVVPGTLTIDPDQVEAAVTPRTRAIMPVHLGHQMADMDRVVQVAR
                     RHSLALVEDCAHAHGQRWRDRGAGSFGELGSFSHQSTKLLTAGEG"
     gene            733..1938
                     /locus_tag="VEP73_11545"
     CDS             733..1938
                     /locus_tag="VEP73_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HYT27103.1"
                     /translation="MADRPLTAGTPSLLRAINARTILELIQRTSPVSRAQVARDSGLS
                     KPTVSLGLAALLDAGLVREVGRSSGRRGPSAVLYELNPEAGWVAGIHVGRHWVRAALA
                     DITGTIVARRHERARASSAQALIGQIGAIAHGLAAEAGIRWDQVSHVTVGGPGVFEPT
                     RGAVILAPNLPGWGRQGLVEMLRGELGGRIGVENDVNLAAVGERWKGIGQGVRDFGFL
                     SVGTGVGMGLVLDGQLYRGSSGAAGEVGYLPVGADPHDRNVRRRGAFEEATGSAGVVR
                     VAREAGMTGSLTPKKVFALARKGDPLAARTVEVEARHLALGLAVVTAVVDLELVILGG
                     GIGGNADLLLEPVERELRALSPLRPRLAASALGGDAVLQGALATALATAQDRLFDPAR
                     MLDRKELVV"
     gene            1999..>2185
                     /locus_tag="VEP73_11550"
     CDS             1999..>2185
                     /locus_tag="VEP73_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27104.1"
                     /translation="MRRRLIGLTALALLVAACTAGGGSSKPVQTANTSANHAPLTLTV
                     WSNFTSREYKVTTNALNM"
BASE COUNT          288 a          827 c          800 g          270 t
ORIGIN      
        1 ccctcgccgg ccgtgaggag cttggtggac tggtggctga aggagccgag ctccccgaac
       61 gagccggcgc cccggtcgcg ccagcgctgg ccgtgggcgt gggcgcagtc ctccaccagc
      121 gccagcgagt ggcgccgcgc gacctggacg acccggtcca tgtcggccat ctggtggccg
      181 aggtggaccg gcatgatcgc gcgcgtccgc ggggtgacgg cggcctcgac ctggtcgggg
      241 tcgatggtga gcgtcccggg gacgacgtcg cagaacaccg ggagcgcgcc ggcggccatg
      301 gccgcgtagg gcgtggccga gaaggtcagc gcggggacga tcacctcgtc gccccagccg
      361 atcccgaggg ccttgagcgc gacctccatg gtgacggtgc cgttggacat caccacgccg
      421 tgcctggacc cctggtaggc ggcgaagcgc tcggcgaact cggcggcgag cggcccgggc
      481 tcggggaagc cgccccagcg gccgccgcgc accacgctcg cgaccgcctc cacgtcgcgc
      541 tcgtcgtgca ccggccacgc cgggtagccc tggggccgga ccggcgcccc gccacgcacc
      601 gcaagctccg ccatctcgct cctccaggtc gagccacgtc ccccgtggca cccgcacggg
      661 gttgacttcc ccgccgcgcc gccgataatg tcggcgtgag gaaggtttcc taactataac
      721 aggggagacg ccttggccga ccggccgctc acggcgggca cgccgagcct gctccgcgcg
      781 atcaacgccc gcaccatcct cgagctgatc cagcgcacca gccccgtctc gcgcgcgcag
      841 gtggcccggg acagcggcct gtccaagccg accgtctcgc tgggcctcgc ggccctcctg
      901 gacgccgggc tggtgcgaga ggtgggccgc tccagcgggc gccgcgggcc cagcgcggtg
      961 ctctacgagc tgaacccgga ggccggctgg gtggcgggca tccatgtcgg ccgccactgg
     1021 gtccgcgcgg cgctcgccga catcaccggc accatcgtgg cccgccgcca cgagcgggca
     1081 cgggcctcga gcgcccaggc gctcatcggc cagatcggcg cgatcgccca cgggctcgcc
     1141 gccgaggccg ggatccgctg ggaccaggtg agccacgtca ccgtcggcgg cccgggcgtg
     1201 ttcgagccga cccgcggcgc ggtcatcctc gcccccaacc tgccgggctg gggccgccag
     1261 ggcctggtcg agatgctgcg gggcgagctg ggcggccgca tcggggtgga gaacgacgtg
     1321 aacctggccg cggtcggcga gcgctggaag ggcatcggcc agggcgtgcg cgacttcggc
     1381 ttcctctcgg tcggcacggg cgtcggcatg gggctggtgc tcgacggcca gctctaccgg
     1441 ggctccagcg gcgccgccgg cgaggtcggc tacctgccgg tcggcgccga cccccacgac
     1501 cgcaacgtcc ggcgccgcgg cgcgttcgag gaggccacgg gcagcgccgg cgtggtccgc
     1561 gtcgcgcggg aggccggcat gacggggtcg ctcacaccca agaaggtgtt cgcccttgca
     1621 cgcaagggcg acccgctggc cgcgcggacg gtcgaggtcg aggcacggca cctcgccctc
     1681 ggcctggccg tggtgaccgc cgtggtcgac ctcgagctgg tcatcctcgg tggcggcatc
     1741 ggcggcaacg ccgacctgct gctcgagccg gtcgagcggg agctgcgcgc gctctcgccg
     1801 ctgcggccgc ggctcgcggc ctcggcgctc ggcggggacg ccgtgctgca gggcgccctc
     1861 gccaccgccc tcgccaccgc ccaggaccgt ctgttcgatc ccgcacgcat gctcgaccgg
     1921 aaggagctcg tggtctgaag tccaggtggt ctgaagtcca accaagcggc agcaccttcg
     1981 ccccgaaggg agtctcacat gagacgacgg ctcatcggcc tcaccgcact ggcgctgctc
     2041 gtcgccgcct gcacggcggg cggtggcagc tccaagcccg tgcagacggc caacaccagc
     2101 gccaaccacg ccccgctgac gctcacggtg tggagcaact tcacctcgcg cgagtacaag
     2161 gtcaccacca acgcgctcaa catga
//
LOCUS       DASPYY010000737         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1106403, whole genome shotgun sequence.
ACCESSION   DASPYY010000737 DASPYY010000000
VERSION     DASPYY010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106403"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VEP73_11555"
     CDS             <1..1013
                     /locus_tag="VEP73_11555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYT27105.1"
                     /translation="ASISAFDKDPVDYYTLACAGMNSWSRFAGRLGGDVGYRRGGQIR
                     WTAGPGDDAWLAERVARAQAWGYPIQLVSERQLRDLLPAAEPGPVGMAAHAARDAHVD
                     PPAVLAACHAVLREAGARLLLGARAAVRVVNDGVRVQVGDETLSPSVTVLATGAGSVA
                     MAAPFGLDIPLVPSPGMLVVTTPTAPLTPGVVYLPGDPGPPVHLRHRADSAVLIGQRS
                     QEEVATDLSGRHARAMVAQAARFFPTLGASRVERVQVGWRSLPADRLPIVGPVPGEPS
                     LYLAVTHSGVTLAPALGRLVARELLDHVEEPLLTPFRPGRFSARAARALLDVEQAFQA
                     MS"
     gene            1051..1314
                     /locus_tag="VEP73_11560"
     CDS             1051..1314
                     /locus_tag="VEP73_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HYT27106.1"
                     /translation="MLLRLGPLGRGEILGPAPDAGSGRAAGAVFEIPLADPAASLNYV
                     IHQPSGDSVPATREPGGDRSFVPAISPEVWVVSGDPTIHTSKP"
     gene            1502..>3084
                     /locus_tag="VEP73_11565"
     CDS             1502..>3084
                     /locus_tag="VEP73_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYT27107.1"
                     /translation="MNGWRDRGERLLETGLALSSELSLPAILQRIVDLAAEITRARYA
                     ALGVLGPDGTITEFITTGVTAAERAAIGPLPVGRGILGVLIDDARTLRLRAIADDPRS
                     VGFPPNHPPMRSFLGAPVAARGTVFGNIYLTGKQGADEFDADDERALEVLAAQAGVAI
                     ENARLYEEAHHRAQRLEAIRAITAAILAGTELDAALDLVARHARELVGADLTVIATPA
                     EGGTLALEAADGEHAAELRGTAFPVQGSVSGEVIRTGKVVVLADASADERVWQPIVRT
                     GEIGPALFVPLAAAGRPFGTLMVANVVGGARFGEADVGLTETFAEQASVVLEHARLRR
                     ELERLAVLEDRERIAKELHDGAIQSLFAVGLGLQGTASLAGAADIRGRIEAAVEELDH
                     VIRDLRSYIFGLRPGILADRQLDQALRQLVEEFQERTGIVAVAEIDPAVAAELAHRAA
                     DLVQLTREALSNVSRHADAPTCRVSLYADGGGNGVLEIEDDGHGFDPDNPAHVGHGMR
                     NLRDRAQALGGHAEISSVIS"
BASE COUNT          384 a         1163 c         1126 g          411 t
ORIGIN      
        1 tcgcgtcgat cagcgcgttc gacaaggacc ccgtcgacta ctacacgctg gcgtgtgcgg
       61 gcatgaacag ctggtcgaga tttgccgggc ggctcggcgg cgacgtcggc taccgccgcg
      121 gcggccagat ccgttggacc gccggcccgg gagacgacgc gtggctcgcc gagcgggtcg
      181 cgcgggccca ggcctggggc tacccgatcc agctggtctc cgagcgccag ctccgcgacc
      241 tgctcccggc ggccgagccc ggcccggtcg gcatggccgc gcacgccgcg cgcgacgctc
      301 acgtcgatcc cccggccgtg ctggccgcct gtcacgccgt cctccgcgag gctggcgccc
      361 ggctcctgct cggcgcgcgg gccgcggtcc gcgtcgtcaa tgacggcgtg cgcgtccagg
      421 ttggcgacga gacactctcg ccctcggtca ccgtgctcgc gacgggggcc gggtcggtcg
      481 cgatggctgc ccccttcggc ctcgacatcc cgctcgtgcc gagcccgggg atgctggtcg
      541 tcacgacgcc gaccgccccg ctgaccccgg gggtcgtcta cctgcccggc gacccgggac
      601 caccggtgca cctgcgccac cgggcggaca gcgccgtgct gatcggccag cgatcgcagg
      661 aggaggtcgc caccgacctg tccgggaggc atgcgcgggc catggtcgcc caggccgccc
      721 gcttcttccc gaccctcggc gccagccgtg tcgagcgggt gcaggtcgga tggcggtcgc
      781 tgcccgccga ccgcctgccg atcgtcgggc cagtacccgg cgagccctcc ctctatctgg
      841 ccgtcaccca cagcggggtc accctcgcgc cggcgctcgg acgcctggtc gccagggagc
      901 tgctcgacca tgtcgaggag cccctgctca cgccgttccg gcccggccgg ttctcggccc
      961 gggcggcccg agcgctcctc gatgtcgaac aggccttcca ggccatgagc tgacccggac
     1021 ccggaccccg acccaggcca tggccgctcg gtgctcctgc ggcttgggcc gcttggccgg
     1081 ggtgaaatcc tggggccggc tccggatgct gggtctgggc gcgccgccgg cgctgtgttc
     1141 gagatcccgc tcgccgatcc ggcggcctcg ctgaactacg tcatccacca gccgagcggc
     1201 gactcggtgc cggccacgcg cgagccgggc ggcgaccgct cgttcgtccc cgccatcagc
     1261 ccggaggtgt gggtggtcag cggcgacccg acgatccaca cctccaagcc gtaagccgca
     1321 gatcgacaga tgccaggaca ggtcgtggtg gcaggtcgcc gaagtcgcca ctgctggcga
     1381 tcgagtcatc gttggacgag gttctccggc cggccggcca ggaacgcggc gacgttacgg
     1441 gcggcaccag cctaccggga ccccgacgcc gggggcgttt gcgggatgct acgctccgtt
     1501 gatgaacggc tggcgggacc gcggcgaacg gctcctcgag actgggctgg ccctgtcctc
     1561 ggagctctcg ctgccggcga tcctgcagcg catcgtcgac ctcgccgccg agatcaccag
     1621 ggcccgctat gcggccctcg gcgtgctggg accggacggc accatcaccg agttcatcac
     1681 caccggcgtc accgcggccg agcgggccgc gatcggcccg ctcccggtcg gccgcggcat
     1741 cctgggcgtg ctgatcgacg acgccaggac gttgcgcctg cgcgccatcg ccgacgaccc
     1801 ccggtcggtc gggttcccgc cgaaccaccc gccgatgcgc tccttcctgg gcgcgccggt
     1861 ggctgcccgc ggcacggtct tcgggaacat ctacctgacc gggaagcagg gcgcggacga
     1921 gttcgacgcc gacgacgagc gcgccctcga ggtgctggcc gcgcaggccg gggtggcgat
     1981 cgagaacgcc cgcctgtacg aggaggccca ccaccgcgcg cagcgcctgg aggctatccg
     2041 cgcgatcacg gcggcgatcc tggccgggac cgagctcgac gccgcgctcg acctggtcgc
     2101 acgccacgcg agggagctgg tcggcgcgga cctcaccgtg atcgcgacgc cggcggaggg
     2161 cggcacgctc gccctggagg cggccgacgg ggagcacgcg gccgagctgc gcggcaccgc
     2221 gttcccggtc caggggtcgg tgtccgggga ggtcatccgg accggcaagg tcgtagtgct
     2281 cgccgacgcg tcggccgacg agcgggtctg gcagccgatc gtccgcacag gcgagatcgg
     2341 gccggcgctg ttcgtcccgc tcgccgcggc cgggcggccg ttcggcaccc tgatggtcgc
     2401 caacgtggtc ggcggcgcac gcttcggtga ggccgacgtc gggctcaccg agacgttcgc
     2461 cgagcaggcg tcggtggtcc tcgagcatgc gcgcctgcgg cgcgagctgg agcggctggc
     2521 cgtgctcgag gaccgcgagc ggatcgccaa ggagctgcac gacggcgcca tccagtcgct
     2581 gttcgccgtg ggcctcggcc tgcaggggac cgcctcgctg gccggcgccg ccgacatccg
     2641 cggccggatc gaggcggccg tggaggagct cgaccacgtg atccgcgacc tgcgcagcta
     2701 tatcttcggg ctccgccctg gcatcctggc cgaccgccag ctcgaccagg ccctgcgcca
     2761 gctcgtcgag gagttccagg agcggaccgg catcgtcgcg gtcgccgaga tcgacccggc
     2821 ggtggcagcg gagctggccc accgggccgc cgacctggtg cagctcaccc gggaggcgct
     2881 gtccaacgtc agcaggcacg cggacgcgcc cacgtgccgg gtgtcgctgt acgccgacgg
     2941 cggcggcaac ggcgtgctgg agatcgagga cgacggccac gggttcgacc ccgacaaccc
     3001 ggcgcacgtc ggccacggca tgcggaacct ccgtgatcgt gcccaggccc tcggcggcca
     3061 cgccgagatc tcgagcgtca tctc
//
LOCUS       DASPYY010000738         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_263948, whole genome shotgun sequence.
ACCESSION   DASPYY010000738 DASPYY010000000
VERSION     DASPYY010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263948"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VEP73_11570"
     CDS             complement(<1..721)
                     /locus_tag="VEP73_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT27108.1"
                     /translation="MTQEGRAISVLDALPAGVTGIDTRMGGQSEITAGFLVSGERPAL
                     IETGPSRVASEVADGVARAGLPPGDLAWIVVTHVHLDHAGGVGDLVRTFPNATVVVHP
                     AGARHLADPERLLASSARVYGELMDTVYGGLTPVEAGRIKAAEDGETLDLGGRRLEII
                     HAPGHAKHHVAVFEPDLGALFAGDAVGVLLPSTGVLRPATPPPDFDRDLAVASLRRFG
                     GRNPAHLVLTHFGPIGPPAERL"
     gene            898..2166
                     /locus_tag="VEP73_11575"
     CDS             898..2166
                     /locus_tag="VEP73_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018804436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYT27109.1"
                     /translation="MLGVGIDWAEEFHLVALGHPDEGVIELSRVEHTPAAVEALIERI
                     ARLEPDPAAVRVVIETRHGLLVERLTDAGYTVLPVNPDLIARRRGPARKKDDAEDARI
                     ACLLALDRHAGLRPLVPHGELAGELRAIARDDERAARDQRRLLNRLRQDLISTFPAAL
                     ELAGDDLGAPTVLRLLERWPTRTQLAQASHDELVAFARAARHGWPDRFADRIQQALAA
                     DHLIPRDYLVRAKAATIRLTAIQLLAIGTQRRAWERRMAELLLGARPTGKSGRRREER
                     PGNAVPGGKVYLSFPGLGDRLAARVAGEIGDHVDQFESPNALQCYAGKAPVTRRSGKS
                     ELVVATRLACNRHLADAVQQWAFCSLRASGWAREFYDAQRARGKHHHAALRALGNRWL
                     EVLWHCLIRGVCYDEATHVANRNRALAKAA"
BASE COUNT          320 a          883 c          834 g          292 t
ORIGIN      
        1 cgaggcgctc ggcgggcggg ccgatcggcc cgaagtgcgt gagcacgagg tgcgcggggt
       61 tgcgcccgcc gaaccggcgc aggctggcga cggccaggtc gcggtcgaag tccggcggcg
      121 gcgtggccgg gcggagcacc ccggtggacg ggaggagcac gccgaccgcg tcgccggcga
      181 agagcgcgcc gagatcgggc tcgaagacgg cgacgtggtg cttcgcgtgg ccgggagcgt
      241 ggatgatctc gagccgccgg ccacccaggt cgagcgtctc gccgtcctcc gccgccttga
      301 tccggccggc ctcgaccggc gtgaggccgc cgtagaccgt gtccatgagc tccccgtaga
      361 cgcgggccga gctcgcgagc aggcgctccg ggtcggcgag atgccgcgcg cccgccgggt
      421 gcacgaccac ggtggcgttc gggaaggtgc gcacgaggtc gcccaccccg ccggcgtggt
      481 cgaggtggac gtgggtgacc acgatccagg cgaggtcgcc gggggggagc cccgctcgtg
      541 cgacgccgtc ggcgacctcg ctcgcgaccc gggacggacc ggtctcgatg agcgcgggcc
      601 gttcgccgct caccaggaag ccggcggtga tctccgactg gccccccatg cgggtgtcga
      661 tgccggtcac gccggcgggc agggcgtcca ggacgctgat ggctctaccc tcctgagtca
      721 tggggtcgcc ccctgggaac gcttgggacg cttgtcctgt gaggactgag gtgagacggc
      781 gtcccagggg gccggccccg gaggcccgac gcccacaagg gtgtcgtccc cagaggactg
      841 gtccatcagc acggcggcgt gcctccaact accccgtcca cgatgcaagg aggcacagtg
      901 cttggtgttg ggatcgactg ggccgaggag ttccacctcg tggccctggg acaccccgat
      961 gagggagtga tcgagctcag ccgggtggaa cacaccccgg ccgcggtcga ggcgctgatc
     1021 gaacggatcg ccaggttgga acccgacccg gccgcggtcc gcgtcgtgat tgagacgcgc
     1081 cacgggctgc tcgtagaacg cctcaccgac gccggctaca cggtcctgcc ggtcaacccc
     1141 gacctgatcg cgcggcgccg tgggcccgcc cgcaagaaag acgacgccga ggacgcccgt
     1201 atcgcctgcc tgctcgccct ggaccgccac gccggcctgc gccccctggt cccgcacggg
     1261 gagctggccg gggagctgcg cgccatcgcc cgcgacgacg agcgcgccgc ccgtgaccag
     1321 cgccgcctgc tcaaccggct gcgccaagac ctcatctcga cgttcccggc cgcgctcgag
     1381 ctcgccggcg acgacctggg cgccccaacc gtgctcaggc tgctggaacg ctggccgacc
     1441 cgcacccagc tcgcccaggc ctcccacgac gagctggtcg ccttcgcccg cgccgcccgc
     1501 cacggctggc ccgaccgctt cgccgaccgt atccagcagg ccctggccgc cgaccatctc
     1561 atcccacgcg actacctggt ccgcgccaag gccgccacca tccgcctgac cgccatccag
     1621 ctgctcgcga tcggcaccca gcggcgcgca tgggagcggc gcatggccga gttgctgctc
     1681 ggcgcccgcc caaccggcaa atccggccgg cggcgagagg agcggccggg gaacgcggtc
     1741 cctggcggca aggtctacct cagcttccca gggctcggtg accgcctcgc cgccagggtc
     1801 gcgggtgaga tcggcgacca tgtcgaccag ttcgagtcgc cgaacgccct gcaatgctac
     1861 gcgggcaagg cgcccgtcac ccgccgctcg ggcaagtccg agctggtcgt ggcgacccgg
     1921 ctggcgtgca accgccacct ggccgacgcc gtccagcagt gggcgttttg cagcctgcgg
     1981 gcctccggtt gggcgcggga gttctacgac gcccaacgcg cccgcggcaa gcaccaccac
     2041 gccgccctgc gcgcgctggg caaccgctgg ctggaggtcc tgtggcactg cctgatccgc
     2101 ggcgtctgct acgacgaggc cacccacgtt gccaaccgca accgcgccct cgccaaggcc
     2161 gcctgagcca gaggttgaca aagggtgtct cacgccgacg cctccttcga ggccggcttg
     2221 cccgcggcga ccgccgcggc ggccttcaac cgggccaccg tggtcggcga catctcggcc
     2281 agctcgaggc cgcgcctggc ggcgagctcg tcgggccgct tcagcgacc
//
LOCUS       DASPYY010000739         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1106654, whole genome shotgun sequence.
ACCESSION   DASPYY010000739 DASPYY010000000
VERSION     DASPYY010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106654"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VEP73_11580"
     CDS             complement(<1..898)
                     /locus_tag="VEP73_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT27110.1"
                     /translation="MAHVIGTAGHVDHGKSALVLALTGTDPDRLAEEKARGMTIDLGF
                     AWTRLPSGREVGFVDVPGHERFIHNMLAGVGGVDCALFVVDATEGWRPQSAEHLAVLD
                     LLGVATGVVALSKTDLCDAATIEAAAREVADRLRGTTLEGAAIVPTAVLPGQAAAGRV
                     GVEALRVALDHALSRAAEPPDLGRPRLPIDRVFSARGSGTVVTGTLSGGALTADVEAE
                     LLPAGRRVRIRALQGHGAPLARAEPARRLAVNLAGVATAEVARGDVLVLPGQWSPTRA
                     ADCRLRCLAAAPAPLRPRGAYLL"
     gene            complement(886..>1921)
                     /gene="selA"
                     /locus_tag="VEP73_11585"
     CDS             complement(886..>1921)
                     /gene="selA"
                     /locus_tag="VEP73_11585"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYT27111.1"
                     /translation="GAAAEALLREATGASAALIANNAAGALLLALGCLARGREVVVSR
                     GELIEIGGEFRLPAIMEAAGVTLVEVGTTNRTHLHDYAQALSGASGAVLAVHPSNYRV
                     EGFTTSPELAALAALAHEHGLPLLHDLGSGLLGGPLGGQLRGPLGAEPTVAGSLAAGA
                     DLVVFSGDKLLGGPQAGLVVGHADLVGRLARHPVARAVRADKLTIAALEATLAAHLAG
                     RRDELPVWRALLLSPADLRPRAAALAAALGPAAQLRDGVSVAGGGSLPGEGLPSVLVE
                     VDPGPASEDALLARLRAGDPPLIARAEHGRVVLDLRTVPPEQDELVAAALRHALGPDP
                     GTDGGGQWRT"
BASE COUNT          251 a          748 c          720 g          202 t
ORIGIN      
        1 agagcaagta ggcgccgcgg gggcggagcg gggcaggagc cgcggccagg cagcgcaggc
       61 ggcagtctgc cgcccgggtc ggcgaccact ggccgggcag caccagcacg tcgccgcgcg
      121 ccacctcggc ggtggcgacg cccgccaagt tgaccgccaa gcggcgggcg ggctcagccc
      181 tggccagcgg cgcgccgtgc ccctgcagtg cgcggatgcg tacccgccgg cccgccggca
      241 gcagctcggc ctcgacgtcg gcggtgaggg ccccgcctga gagggtgccg gtgaccaccg
      301 tgccgctccc acgcgccgag aacacccggt cgatcggcag ccgcggcctc cccaggtcag
      361 gcggctcggc cgcccggctg agcgcgtggt cgagcgcaac ccgcagcgcc tcaaccccga
      421 cccgacccgc cgcagcctgt ccgggcagca ctgcggtggg cacgatcgcc gcgccctcca
      481 gggtggtgcc gcgaagccgg tcggcgacct cccgcgcggc cgcctcgatg gtggcagcgt
      541 cgcacaggtc ggtcttgctg agggcgacca ccccggtagc gacgccgagc aggtcgagca
      601 cggcgaggtg ctcggcggac tgggggcgcc agccctcggt cgcgtcgacc acgaacaggg
      661 cgcagtcgac cccgccgacc cccgccagca tgttgtggat gaagcgttca tggccgggca
      721 cgtccacgaa gcccacctcg cgcccggacg gcagccgggt ccaggcgaag ccgagatcga
      781 tggtcatccc gcgagccttc tcctcagcga gccggtccgg gtcggtcccg gtgagcgcca
      841 gcaccaacgc cgacttgcca tggtccacgt gccctgcggt cccgatcacg tgcgccattg
      901 accacctccg tcggtacccg gatcggggcc gagggcgtgg cggagtgccg cggccaccag
      961 ctcgtcctgt tccggcggga cggtgcgaag gtcgagcacc acccggccgt gctcggcccg
     1021 ggcgatcagc ggcgggtcgc cggcgcggag cctggcgagc agcgcgtcct cgctggccgg
     1081 gccggggtcc acctcgacta ggacgctggg cagcccctcc ccgggcaggc tgccgccgcc
     1141 ggccacgctg acgccgtcgc ggagctgggc agccggcccc agcgccgccg ccagcgccgc
     1201 cgcccgcggg cgcaggtccg cagggctgag cagcagggcg cgccacacgg gcagctcgtc
     1261 gcgcctcccg gcgaggtggg cggccagggt cgcctcaagc gcggcgatgg tgagcttgtc
     1321 ggcacgcacc gcccgggcga ccggatgccg ggcgagccgc ccgaccaggt ccgcatgccc
     1381 caccaccagc ccggcctggg gcccgccgag cagcttgtca cccgagaaca ccaccaggtc
     1441 ggccccggcg gccaggctgc cagcgaccgt cggttccgcc ccaagtggac cccgaagctg
     1501 gccgcctagt gggccgccga gcaaccccga gccgaggtcg tggagcagcg gcaggccgtg
     1561 ctcgtgggcg agcgcggcga gcgcggccag ctccggactg gtggtgaagc cctcgacccg
     1621 gtagttggag gggtggacgg cgaggacggc gccgctcgcc ccgctgagcg cctgggcgta
     1681 gtcgtgcagg tgggtgcggt tggtggtgcc gacctcgacc agcgtgaccc cggcggcctc
     1741 catgatcgcg ggcaggcgga actcaccgcc gatctcgatc agctcgccac gcgacaccac
     1801 cacttcacgg ccacgcgcca ggcagccgag ggccagcagc agcgccccgg ccgcgttgtt
     1861 ggcgatcagc gccgccgacg ccccggtcgc ctcgcgcagc agcgcctcgg ccgccgcgcc
     1921 g
//
LOCUS       DASPYY010000740         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1388540, whole genome shotgun sequence.
ACCESSION   DASPYY010000740 DASPYY010000000
VERSION     DASPYY010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388540"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..659
                     /locus_tag="VEP73_11590"
     CDS             390..659
                     /locus_tag="VEP73_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27112.1"
                     /translation="MRLRVEVPAAEPGVRAWQRVERLAGGYTATWYDRFPGGVTERLH
                     STSDPEGGFASEAPLLLGSTTRQALRQALEQRSGGRLHLDPIGPR"
     gene            773..>1696
                     /locus_tag="VEP73_11595"
     CDS             773..>1696
                     /locus_tag="VEP73_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT27113.1"
                     /translation="MRVMVLGGTRFIGAAIVGELDAHGHELLVIHRGEHEPDGLPAAD
                     HLHASRRDLPHLRGAVDDFGPEAVVDTIAMSAADAETALAAVGGDARLLVLSSMDVYR
                     AFGSVLAGVETDPLPIDETAPVRSERYPYRGRIPGREDYDKLEVEDAYLARGATVCRL
                     PVVYGERDYQRREEPILRRVRAGRTRIPAGAGSWLWTRGYVRDVAAGVRLALESEATV
                     GEVLNLGEGRTWSMGLWARHVLEAAGSAAELVRVPDVLLPEDLETLGTVTQHLLVDSS
                     KASDLLGWDETDPHEALRQSVAWHLAHAPEDA"
BASE COUNT          228 a          651 c          610 g          207 t
ORIGIN      
        1 acagccgcca tgagcgccac cccgcgcggg gaccgggcgg cccgcacccg cgccacccgg
       61 cccgctcgcc ggcccgcgcg gtcgcgccac ccgggcgacc tcgcctggct cgggctgggt
      121 gcggccctgc tcgtgctgag cgcactgccg gtccacgccc accgcatctc gagccctgcg
      181 gcgagcgcgt tccggctccg tcaaccacgc cccgagcatc ccgttcgcga tcgtgtgggt
      241 gcccatgcag ctgggcaact tcctgatcgt ccccgcggcc gccctcaccg cctgctgctc
      301 ggcacgcgca ccacgacggc aagccacgaa cggaccgcgt gatgcccaca gccggcacgc
      361 gcccgagccg accgagccac catgggggca tgcggttgcg ggtcgaggtc cccgccgccg
      421 agccaggcgt gcgggcctgg cagcgggtcg agcggctggc cggcgggtac accgcgacct
      481 ggtacgaccg gttcccgggg ggcgtgaccg agcggctgca ctccacaagc gaccccgagg
      541 gcgggttcgc cagcgaggcg ccgctgctgc tcggctccac cacccgccag gcgctccgcc
      601 aggccctcga gcagcgctcc ggcggccggc tccacctcga cccgatcggg ccacgatgag
      661 ccaaggcgac agccaatatc ggcgcggcca agcacaaacc accgcgctcc ccggctgacc
      721 gtaccccagt acgctgccgg cgcacgcagc tgtcgagcga acggagcctg ggatgcgggt
      781 catggtgctt ggagggacgc ggttcatcgg ggccgcgatc gtcggggagc tcgacgcgca
      841 cggccacgag ctgctcgtca tccaccgggg cgagcacgag ccggacggcc tgcccgccgc
      901 cgaccacctg cacgccagcc gccgcgacct gccccacctg aggggagcgg tcgacgactt
      961 cgggcctgag gcggtcgtcg acaccatcgc gatgagcgcc gccgacgccg agaccgccct
     1021 cgccgccgtc ggcggcgacg cccgcctgct cgtgctgtcg agcatggacg tctaccgcgc
     1081 gttcgggtcc gtcctcgcgg gggtcgagac cgacccgctg ccgatcgacg agaccgcgcc
     1141 cgtccgttcc gagcgctacc cgtaccgcgg ccggataccc ggccgcgagg actacgacaa
     1201 gctcgaggtc gaggatgcct acctcgccag gggggccacc gtgtgccggc tccccgtggt
     1261 gtacggcgag cgtgactacc agcgccgcga ggagccgatc ctccgccggg tgcgggccgg
     1321 ccgcacccgg atcccggccg gggcggggag ctggctgtgg acccgcggct atgtccggga
     1381 cgtggccgcc ggcgtacgcc tcgcgctcga gtcggaggcg accgtcggcg aggtgctgaa
     1441 cctgggcgag ggccggacct ggtcgatggg cctgtgggcc aggcatgtgc tggaggcggc
     1501 cggctcggcc gccgagctgg tccgggtccc cgacgtgctg ctgcccgagg acctggagac
     1561 gctcggcacg gtcacccagc acctcttggt cgattcgagc aaagctagtg atctgctcgg
     1621 atgggacgag acagacccgc atgaggcact gcggcagtcg gtggcttggc atctggccca
     1681 tgcgcccgag gatgcc
//
LOCUS       DASPYY010000741         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2793368, whole genome shotgun sequence.
ACCESSION   DASPYY010000741 DASPYY010000000
VERSION     DASPYY010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793368"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1067
                     /gene="dhaK"
                     /locus_tag="VEP73_11600"
     CDS             75..1067
                     /gene="dhaK"
                     /locus_tag="VEP73_11600"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HYT27114.1"
                     /translation="MKKLINSPETVVTEALEGVAAAHPSLSVDVENKVITRAGGPTPG
                     KVGLVSGGGSGHEPLHGGFVGYGMLDAACPGEVFTSPVPDQMLAATTAVNGGAGVLHV
                     VKNYTGDVLNFQMAAEMAGDEGIEVVPVVINDDVAVEDSLYTAGRRGTGATVFVEKIA
                     GALAEQGAGLAAVAATAREVNERSRSFGVALSSCVVPAAGKPTFELGEAEAELGIGIH
                     GEPGRTRAALTPARELAAVALDAIRADLPVGGDLLVMVNGMGGTPLIELYLMFNEVRK
                     YLEGHGGHVVRNLVGNYITSLEMQGFSVTVCRLTDELTGLWDAPVDTPALRWGR"
     gene            1103..>1705
                     /gene="dhaL"
                     /locus_tag="VEP73_11605"
     CDS             1103..>1705
                     /gene="dhaL"
                     /locus_tag="VEP73_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HYT27115.1"
                     /translation="MDAEVFRAWIVSAAGVIEANRDHLTQLDAAIGDADHGINLSRGF
                     TAVLGALDSAAKTPGAILTVTGNTLISKVGGASGPLYGTAFRRAGKALGGAADVDLPA
                     LSAALEAALAGVQQLGAAREGDKTMVDALAAATRAFSKATAEGASQDDALDSLAEAAS
                     AGATATISMQALKGRASYLGPRSVGHEDPGAASTALILAAL"
BASE COUNT          255 a          591 c          635 g          224 t
ORIGIN      
        1 atccgtgccg acgccgcgat gaccagcctc gcgcccgcag aagcagaacc gcagaaagtc
       61 ggtggagcaa cgtcatgaag aagctcataa acagcccgga gacggtagtc accgaagccc
      121 tcgagggtgt cgccgcggcg catccgtcgc tgtcggtgga tgtcgagaac aaggtgatca
      181 cccgggccgg cggccccacg cccggcaagg tcggcctggt gtccggcggc ggttccggcc
      241 acgagccgct gcacggcggc ttcgtcggct acgggatgct cgacgccgcc tgcccgggcg
      301 aggtcttcac ctcgccggta cccgaccaga tgctcgccgc gaccacggcg gtcaacgggg
      361 gcgcgggcgt gctgcacgtg gtgaagaact acacgggcga cgtgctgaac ttccagatgg
      421 cggccgagat ggccggggac gagggtatcg aggtcgtccc ggtcgtgatc aacgatgacg
      481 tcgcggtgga ggactcgctc tacaccgcgg gccgccgcgg caccggggcc accgtgttcg
      541 tggagaagat cgccggcgcg ctagccgaac agggcgccgg cctggccgcc gtggccgcca
      601 cggcgcgcga ggtcaacgag cggagccggt ccttcggcgt ggcgctcagc tcgtgcgtgg
      661 tgcccgcggc gggcaagccg accttcgagc tcggcgaggc cgaggcggaa ctgggcatcg
      721 gcatccacgg cgagcctggc cggacccggg ccgccctcac cccggcgcgg gaactggcgg
      781 cggtggcgct ggacgccatc cgcgccgacc tgcccgtcgg cggggacctg ctggtcatgg
      841 tcaacgggat gggcggcacg ccgctgatcg agctgtacct gatgttcaac gaggtgcgga
      901 agtacctcga gggacacggc ggccacgtcg tgcggaacct ggtgggcaac tacatcacca
      961 gcctggagat gcagggcttc tcggtgacgg tctgccggct caccgacgag ctgacggggc
     1021 tgtgggacgc gccggtggac acgcctgccc tgcggtgggg acgctaggcg cagtgatttc
     1081 cgccgttcgt cagggagcca tcatggacgc tgaagtcttc cgggcctgga tcgtgtcggc
     1141 cgccggggtc atcgaggcca accgcgacca cctcacccag ctggatgccg cgatcggaga
     1201 cgccgaccac ggcatcaacc tctcgcgcgg gttcaccgcg gtcctaggcg cgcttgactc
     1261 ggcggcaaag acgccggggg cgatcctgac cgtgacgggc aacaccctga tctccaaggt
     1321 cggcggcgct tccgggccgc tgtacggcac ggcgttccgc cgggccggca aggcgctggg
     1381 aggcgccgcc gacgtggacc tgcccgccct gtccgcggcg ctggaggcgg ccctggcggg
     1441 cgtccagcag ctcggcgccg cccgcgaggg cgacaagacc atggtcgacg cgctcgccgc
     1501 ggcgaccagg gccttcagca aggcgaccgc ggagggggcg tcgcaggacg acgccctgga
     1561 ttccctggcg gaggcggcct cggccggcgc caccgcgacc atctccatgc aggcgctgaa
     1621 gggccgcgcc agctacctcg ggccgcgcag tgtcggccat gaggatccgg gagcggcctc
     1681 gaccgcgctc atcctcgccg ctctc
//
LOCUS       DASPYY010000742         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_402697, whole genome shotgun sequence.
ACCESSION   DASPYY010000742 DASPYY010000000
VERSION     DASPYY010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402697"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..580
                     /locus_tag="VEP73_11610"
     CDS             203..580
                     /locus_tag="VEP73_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT27116.1"
                     /translation="MARSTMHIEIPASGAYLSVVRAAATGLAAQLQFTYEEIDDLRIA
                     VDEACAQLLARRGSADTLRIQYHLEPAELWVEVSVEAPDRGDPLERDTFTWQILSALT
                     DEVTERAEADGLHLSFRKCGGRT"
     gene            577..1503
                     /locus_tag="VEP73_11615"
     CDS             577..1503
                     /locus_tag="VEP73_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015507117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HYT27117.1"
                     /translation="MNDMAFDSPTVVGPLDPGRAGGAGPADGQPQGLGRPGHSGDEAG
                     DGAADEDLAELPEGRGVDRVYAKELFQRFVELRDPALREELVGLHLPLVEYLARRFRN
                     RGEPLEDLIQVGTIGLLKAIDRFDLEREVEFSTYATPTIVGELKRHFRDKGWAVRVPR
                     RLQELNLSLNKVVAELSQEVGRSPTIPEIAAKVRLSEEEVLEGLDTSNAYAVVSLDAP
                     AGGEDAPTVSEHIGSEDESIEALEYRAALGPLIAELPERERRILYLRFFRGMTQSQIA
                     ARLGISQMHVSRLLNRTLGVLRAGLLEEDETG"
     gene            1506..2453
                     /locus_tag="VEP73_11620"
     CDS             1506..2453
                     /locus_tag="VEP73_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYT27118.1"
                     /translation="MRLLLVVNPGATRFTGRLRDRVVRTLAAEHKVDVRHTAHPGHAT
                     ELTRQGVADGAEVVVAMGGDGTVNEVVNGLAGSAVPLGLVGGGRANVFARGLGLPGEP
                     VRASARLLELLAAGARRRISLGMADDRRFTFGTGLGLDGAIVREVERRRRAKQLYGDH
                     AYVAAGLKTLLVGYDRNHPHLTVHFADQRPAMSGFFALVGNGDPYSYLGPRPFRPTPQ
                     ASYELGLDILIGQSMSGLAITRALTGMLSPRPRPTHPSFPVLHDQHGFRLETDIPLAF
                     QLDGEYLGDRTSVAVEHLLDALVVVAPGAGRPRGTAARR"
     gene            2450..>2921
                     /locus_tag="VEP73_11625"
     CDS             2450..>2921
                     /locus_tag="VEP73_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYT27119.1"
                     /translation="MSARTACVAHVGSGEFGRVVDALAELRGRFGRIPVYPVQAPGEA
                     GEVVRELAPGLDLLAVFGGDGTIHDAAGGLPLGPDGPAVALLPGGTGNDLVRGLGLPL
                     DAVAAARAATAGAPRALDLLDCDGDRAVNGVNAGFAAAATDVLSRRAKLLLGPAA"
BASE COUNT          380 a         1088 c         1076 g          377 t
ORIGIN      
        1 cccgcccgcg gacgcaccct gtgggtggcg gcgaccgggt gaggcggcac gtgccggagt
       61 actggcaaga tcggccgcct gctggttgca tgggtcccgc cgggacggtt ggatggtctt
      121 gctccgccgg tgcgtcgccg cggggccgga cgagagtttt ggagcgccgg gcactccgtg
      181 cgccgaagga gccgcgagcg ccatggccag gtcgaccatg cacatcgaga tccccgcatc
      241 aggagcctac ctgtctgtgg tgcgggcagc cgccaccggc ctcgcggcgc agctccagtt
      301 cacctacgag gagatcgacg acctccgcat cgccgtggac gaggcgtgcg cccagctcct
      361 cgcccggcgc ggcagcgcgg acacgctgcg catccagtac cacctggaac cggccgagct
      421 gtgggtggag gtgtcggtgg aggcgcccga ccgtggcgac ccgctggagc gggacacctt
      481 cacctggcag atcctgagcg cgctcaccga cgaggtgacc gagcgggccg aggcggacgg
      541 gctccacctc agcttccgga agtgcggcgg gaggacgtga acgacatggc cttcgactcg
      601 cccacggtgg tgggacccct ggaccccggc cgggccggcg gcgccggccc ggcggacggg
      661 cagccacaag ggctgggccg tccgggtcac tccggcgacg aggccggcga cggggccgcc
      721 gacgaggacc tcgcggagct tcccgagggg cgcggggtcg accgcgtcta cgccaaggag
      781 ctgttccagc gcttcgtcga gttgcgcgac ccggcgctgc gcgaggagct ggtcggcctc
      841 cacctgcccc tggtcgagta cctggcgcgt cgcttccgca accgcgggga gccgctcgag
      901 gacctgatcc aggtcggaac gatcggactc ctgaaggcga tcgaccgctt cgacctcgag
      961 cgcgaggtcg agttctcgac ctacgcgacg ccgaccatcg tcggggagct caagcggcac
     1021 ttccgcgaca agggctgggc ggtccgggtc ccacgacggc tccaggagct caacctgtcg
     1081 ctcaacaagg tcgtggccga gctctcacag gaggtcggcc ggtcgccgac catcccggag
     1141 atcgcggcca aggtgcgcct gagcgaggag gaggtgctcg aggggctgga cacctcgaac
     1201 gcctacgccg tggtgtcgct ggacgcgccg gccggcggcg aggacgcccc cacggtgagc
     1261 gagcacatcg gctccgagga cgagagcatc gaggcgctcg agtaccgggc ggcgctgggg
     1321 ccgctgatcg ccgagctgcc cgagcgggag cgccgcatcc tctacctgcg cttcttccgc
     1381 ggtatgaccc agtcgcagat cgctgcccgc ctcggcatct cccagatgca cgtctcccgc
     1441 ctgctcaacc ggacgctcgg cgtgctgcgg gccggcctgc tcgaggaaga cgagacaggc
     1501 tgagcatgcg gctgctgctc gtcgtcaacc cgggagcgac caggttcacc gggcgcctgc
     1561 gcgaccgcgt ggtccggacg ctcgccgccg agcacaaggt ggacgtgcgc cacaccgccc
     1621 acccgggcca tgcgaccgag ctcactcgcc agggcgtcgc cgacggggcc gaggtggtcg
     1681 tggcgatggg tggcgacggg acggtcaacg aggtggtcaa cggcctcgcc gggtccgccg
     1741 tgccgctcgg gctcgtcggc ggcggcaggg ccaacgtgtt cgcccggggg ctcgggctgc
     1801 ccggcgagcc cgtgcgggcc tccgcgaggc tcctcgagct gctcgcggcc ggtgcccggc
     1861 ggcgcatctc cctcgggatg gcggacgacc ggcgcttcac cttcgggacc ggcctcggcc
     1921 tcgacggcgc gatcgtccgc gaggtcgagc gccggcgccg ggcgaagcag ctctacgggg
     1981 accacgccta cgtcgccgcc ggactgaaga cgctgctcgt cggctacgac cgcaaccatc
     2041 cgcacctgac cgtgcacttc gccgaccagc ggccggccat gagcggcttc ttcgccctgg
     2101 tcggcaacgg cgacccctac agctacctcg gtccccgccc cttccggccg acgccccagg
     2161 ccagctacga gctgggactc gacatcctga tcgggcagtc catgtccggc ctcgcgatca
     2221 ccagggcgct gaccggcatg ctgtcgccgc ggccgcggcc cacccacccg agcttcccgg
     2281 tgctgcacga ccagcacggc ttccggctcg agaccgacat ccccctcgcg ttccagctcg
     2341 acggcgagta cctgggcgac cggaccagcg tggccgtcga gcacctgctc gacgcgctcg
     2401 tcgtggtggc gccaggggcg gggcgcccgc gcgggacggc ggcgcgccgg tgagcgcgcg
     2461 gacggcatgc gtcgcgcacg tgggctccgg ggagttcggc cgcgtggtgg acgcgctcgc
     2521 cgagctgcgc gggcgcttcg gcaggatccc cgtctacccg gtccaggcgc ccggcgaggc
     2581 cggcgaggtc gtgcgcgagc tggcccccgg cctcgacctg ctcgccgtat tcggtggcga
     2641 cggcaccatc cacgacgcgg ccggcgggct cccgctcggg ccggacggcc ccgccgtggc
     2701 gctgctgcca ggcgggaccg gcaacgacct cgtccgcggc ctcggcctgc ccctcgacgc
     2761 ggtcgccgcc gcccgggcgg cgaccgccgg cgccccccga gcgctcgacc tgctcgactg
     2821 cgacggcgac agggcggtca acggtgtcaa cgcggggttc gccgctgccg ccaccgacgt
     2881 cctgtcccgg cgggccaagc tgctcctcgg gccggccgcc t
//
LOCUS       DASPYY010000743         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2652347, whole genome shotgun sequence.
ACCESSION   DASPYY010000743 DASPYY010000000
VERSION     DASPYY010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652347"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="rpsC"
                     /locus_tag="VEP73_11630"
     CDS             <1..613
                     /gene="rpsC"
                     /locus_tag="VEP73_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYT27120.1"
                     /translation="VKGAETDAQLVAQGVAEQLASRVSFRRAMRRAVNSAMKGGAKGI
                     RVQCSGRLGGAEMARTEWYREGRVPLHTLRADIDYGLAEGRTTFGQIGVKVWVYRGDV
                     LPSREEQEAERARARARASAGGRDDRPRGRGRGGNGGGARPDGPARGAGGGPGAEAGS
                     RPAARTRAKPAQPAGGSADAPAAETGPRTGPPAGQAAHEQAKE"
     gene            620..1042
                     /gene="rplP"
                     /locus_tag="VEP73_11635"
     CDS             620..1042
                     /gene="rplP"
                     /locus_tag="VEP73_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYT27121.1"
                     /translation="MLIPKRVKHRKQHRGHRRGVASGGTTVAFGDFGLQALEPSWVTN
                     RQIEAARIAMTRYIRRGGKVWINVYPDKPVTKKPAETRMGSGKGSPEWWVAVVRPGRV
                     MFELAGVPEATAQEAMRLAAHKLPMKCKFVKRADMAES"
     gene            1042..1317
                     /gene="rpmC"
                     /locus_tag="VEP73_11640"
     CDS             1042..1317
                     /gene="rpmC"
                     /locus_tag="VEP73_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYT27122.1"
                     /translation="MTMKATELADLDAEELAAKLAEAKEELFNLRFQNVTGQLDNPHR
                     LREVRKDIARIFTVIRKRELAGEAVPVAAAPERRQARRRFGRRQRKG"
     gene            1324..1605
                     /gene="rpsQ"
                     /locus_tag="VEP73_11645"
     CDS             1324..1605
                     /gene="rpsQ"
                     /locus_tag="VEP73_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYT27123.1"
                     /translation="MTASTPEQATNRGDRKVRLGVVVSDKMDKTVVVEVEDVRQHRLY
                     HKTIRRTTRLKAHDEQNEAGTGDIVRLAETRPTSATKRWRVVEVIQKAK"
     gene            1625..>1864
                     /gene="rplN"
                     /locus_tag="VEP73_11650"
     CDS             1625..>1864
                     /gene="rplN"
                     /locus_tag="VEP73_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYT27124.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSGRRYAGIGDIIVATVK
                     DALPGAGVKKGDVVKAVVVRTRKERRRADGSYVRFD"
BASE COUNT          312 a          637 c          715 g          200 t
ORIGIN      
        1 ggtcaagggc gccgagaccg acgcccagct cgtcgcccag ggcgtcgccg agcagctcgc
       61 gagccgggtc tcgttccgcc gggccatgcg ccgcgcggtc aactcggcca tgaagggcgg
      121 cgcgaagggc atccgggtgc agtgctcggg ccgcctgggc ggcgccgaga tggcccgcac
      181 cgagtggtac cgcgagggcc gcgtgcccct gcacaccctc agggccgaca tcgactacgg
      241 gctcgccgag ggccgcacca ccttcggcca gatcggcgtc aaggtgtggg tctaccgcgg
      301 cgacgtcctg ccctcgcgcg aggagcagga ggccgagcgg gcccgcgcca gggcccgggc
      361 atcggccggc gggcgggacg accggccccg cgggcggggc cggggcggca acggcggcgg
      421 tgccaggccc gacggcccgg ccaggggtgc tggcggcggc ccgggcgccg aggcgggtag
      481 caggccggcc gccaggacgc gggccaagcc ggcccagccg gcgggcggca gcgcggacgc
      541 gccggccgcc gagacggggc cgaggaccgg gccccccgcc ggccaggccg cgcatgagca
      601 ggcgaaggag tagggacaga tgctcattcc gaagcgtgtg aagcaccgca agcagcaccg
      661 cgggcatcgc cgcggcgtcg cgagcggcgg caccacggtg gccttcgggg acttcggcct
      721 gcaggcgctg gagccctcct gggtcaccaa ccggcagatc gaggccgccc gtatcgccat
      781 gacccggtac atccgccgtg gcgggaaggt gtggatcaac gtctaccccg acaagccggt
      841 gacgaagaag cccgccgaga cccgcatggg gtctggcaag ggctcgcccg agtggtgggt
      901 cgccgtggtc aggcccggcc gggtgatgtt cgagctcgcc ggcgtgccgg aggcgaccgc
      961 ccaggaggcg atgcgcctgg ccgcccacaa gctgcccatg aagtgcaagt tcgtgaagcg
     1021 cgcggacatg gcggagtcgt gatgaccatg aaggcaaccg aactggccga cctggacgcc
     1081 gaggagctcg cggccaagct ggccgaggcg aaggaagagc tgttcaacct ccgcttccag
     1141 aacgtcaccg gccagctgga caacccgcac cggttgcgcg aggtccgcaa ggacatcgcg
     1201 cggatcttca cggtcatccg caagcgcgag ctggccggcg aggccgtgcc ggtggccgcc
     1261 gcgcccgagc gccgccaggc gaggcgccgc ttcggccgcc ggcagaggaa ggggtagcga
     1321 gacatgactg cgagcacgcc ggaacaggcg acgaaccgcg gcgaccgcaa ggtgcgcctc
     1381 ggggtcgtcg tctccgacaa gatggacaag acggttgtcg tcgaggtcga ggacgtccgc
     1441 cagcaccggc tgtaccacaa gaccatccgg cgcacgaccc ggctgaaggc gcacgacgag
     1501 cagaacgagg ccggcacggg cgacatcgtc cgcctggccg agacccggcc gacgtcggcc
     1561 accaagcgct ggcgggtcgt cgaggtcatc cagaaggcca agtgagagtg gataagaggc
     1621 caagatgatt cagcaggagt cgcggctcaa ggtcgcggac aacacgggtg cgaaggagat
     1681 cctctgcatc cgcgttctcg gtggttcggg tcggcgctac gcgggaatcg gcgacatcat
     1741 cgtcgcgacc gtgaaggacg cacttcccgg cgccggcgtg aagaagggtg acgtcgtcaa
     1801 ggccgtcgtc gtgcgcaccc gcaaggagcg ccggcgcgcc gacggctcct acgtccgctt
     1861 cgac
//
LOCUS       DASPYY010000744         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2512659, whole genome shotgun sequence.
ACCESSION   DASPYY010000744 DASPYY010000000
VERSION     DASPYY010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512659"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2349)
                     /locus_tag="VEP73_11655"
     CDS             complement(<1..2349)
                     /locus_tag="VEP73_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYT27125.1"
                     /translation="MAQGPLPGEAAAEEGAVGTMTGTSVEMGRYVGSPLLRKEDAPLI
                     TGYGHFVDNLALPGMVWVAVVRSPMAHARITRVDTSAALGRPGVVAAYSGADLASEWA
                     AGLPCAWPVAPETRIPTHWPVAQDKARLAGDPVAVVVADTSEHAVDAAEAVDVDYGQL
                     PAVTSIEEALAEGAPVIHEEFGDNICFKDLKIAAGDVDKVFAEAPVTVKERYYHPRLI
                     PNAMEPRGVLVQPLPPTGEMTMWSSTQIPHILRTLLALTLGISESKFRVIAPDVGGAF
                     GSKLDVYNEEAICLAVARKLGVPVKWIEERSEGYVATIHGRDVIQDMEVAATEEGRLL
                     GVRAKVLCDMGAYCQLVAPGIQVLGATLFSGVYKAEAASFECTAVFTNRTPTDAYRGA
                     GRPEATYAIERIMDALARRVGKDPAELRRMNFIEPFSEPTANVFGALYDSGNYVAGLD
                     KALRLADYGGLRREQAERRERGDVKQLGIGLSTYIEACGSAPSRGFGAVNFGAGGWEA
                     ATIRCLPTGKVVAVTGTSPHGQGHETSWSQIVADALGVKFEDVEVLHGDTAISPQGLD
                     TYGSRSLAAGGVALQKAAEKVVAKAKKIAAHELEVAEEDVEFAEGKLRVRGAPDKAKT
                     IADIAFSAFLAHDLPDGVEPGLEESALFDPVNNTFPSGAHICVVEVDTETGLADIVRY
                     VAVDDCGNIVNPLIAQGMVHGGVAQGIAEGLYEEATYDEDGNLTSSHMGYYRVPSAAE
                     LPPFVTDHVVTPSSSNPGGWKGVGEAGTIGSPPAVVNAVIDAL"
     gene            complement(2363..2845)
                     /locus_tag="VEP73_11660"
     CDS             complement(2363..2845)
                     /locus_tag="VEP73_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT27126.1"
                     /translation="MRRIALTVNGVRREAEVEPRQLLVYFLRETLGLTGTNVGCDTSS
                     CGACTVLVDGESVKSCTMLAVQADGTEVTTIEGLARNGELHPMQEAFRQNHALQCGYC
                     TPGMIMAAVGLVDSGAGMSDREIRLGLEGNLCRCTGYHNIVKAIQSAAGTMRGAPADA
                     "
BASE COUNT          387 a         1056 c         1068 g          457 t
ORIGIN      
        1 gagcgcgtcg atcaccgcgt tgacgacggc cggtggggag ccgatggtcc cggcctcgcc
       61 gacgcccttc cacccgcccg ggttgctgct ggagggggtg acgacgtggt cggtgacgaa
      121 ggggggcagc tcggccgctg acggcacccg gtagtagccc atgtggctcg aggtgaggtt
      181 gccgtcctcg tcgtaggtgg cctcctcgta gagcccctcg gcgatgccct gggcgacccc
      241 gccgtggacc atgccctgcg cgatcagcgg gttgacgatg ttcccgcagt cgtcgacggc
      301 cacgtacctg acgatgtcgg ccaggccggt ctcggtgtcg acctccacca cgcagatgtg
      361 ggcgcccgac gggaaggtgt tgttcaccgg gtcgaacagg gcgctctcct cgagccctgg
      421 ctcgacgccg tcgggcaggt cgtgggcgag gaaggccgag aaggcgatgt ccgcgatggt
      481 cttcgccttg tcgggcgcgc ccctgacccg gagcttgccc tcggcgaact cgacgtcctc
      541 ctcggccacc tcgagctcgt gggcggcgat cttcttcgcc ttggcgacga ccttctcggc
      601 cgccttctgc agggcgacgc cgccggcggc caggctgcgg ctgccgtagg tgtccaggcc
      661 ctggggcgag atcgcggtgt cgccgtgcag cacctcgacg tcctcgaact tgacccccag
      721 ggcgtcggcc acgatctgcg accaggaggt ctcgtggccc tggccgtgcg gggaggtgcc
      781 ggtgaccgcg accaccttgc cggtcggcag gcagcggatg gtggccgcct cccagccgcc
      841 tgccccgaag ttcacggcgc cgaagccccg cgacggggcg ctgccgcacg cctcgatata
      901 ggtggacagg ccgatgccga gctgcttcac gtcgccccgc tcgcgccgct cggcctgctc
      961 gcggcgcagg cccccgtagt cggccagccg gagcgccttg tcgagccctg ccacgtagtt
     1021 cccggagtcg tacagggcgc cgaagacgtt cgcggtcggc tccgagaacg gctcgatgaa
     1081 gttcatccgc cgcagctcgg ccgggtcctt gccgacccgc cgggcgagcg cgtccatgat
     1141 ccgctcgatc gcgtaggtcg cctcgggccg gcccgcgccc cggtaggcgt cggtcggcgt
     1201 ccggttggtg aacaccgcgg tgcactcgaa ggaggccgcc tcggccttgt agacgccgct
     1261 gaacagggtc gcgccgagga cctggatgcc cggggcgacg agctggcagt aggcgcccat
     1321 gtcgcacagc accttggccc gcacgccgag cagcctgccc tcctcggtcg ccgcgacctc
     1381 catgtcctgg atcacgtcgc ggccgtggat cgtggccacg tagccctcgg agcgctcctc
     1441 gatccatttg accggcacgc cgagcttgcg cgcgacggcc aggcagatcg cctcctcgtt
     1501 gtagacgtcg agcttggagc cgaaggcgcc gccgacatcg ggggcgatga cgcggaactt
     1561 cgactcggag atgccgagcg tgagcgcgag cagggtgcgc aggatgtgcg ggatctgggt
     1621 cgacgaccac atggtcatct cgccggtggg cggcagcggc tggacgagca ccccgcgcgg
     1681 ctccatggcg ttcgggatca gccgcgggtg gtagtagcgc tccttcacgg tcaccggcgc
     1741 ctcggcgaac accttgtcga cgtcgccggc ggcgatcttc aggtccttga agcagatgtt
     1801 gtcgccgaac tcctcgtgga tgaccggggc gccttcggcg agcgcctcct cgatcgaggt
     1861 cacggccggg agctgcccgt agtcgacgtc gaccgcctcg gcggcgtcga ccgcgtgctc
     1921 gctggtgtcg gcgaccacca cggcgaccgg gtcgccggcc aggcgcgcct tgtcctgcgc
     1981 gaccggccag tgggtcggga tcctggtctc gggcgcgacc ggccaggcgc agggcagccc
     2041 agcggcccac tccgaggcca ggtcggcgcc cgagtaggcg gcgaccacgc cgggccgccc
     2101 gagcgcggcc gacgtgtcca cgcgggtgat gcgggcgtgc gccatggggc tgcggacgac
     2161 ggcgacccag accatgccgg gcagggccag gttgtcgacg aagtggccgt acccggtgat
     2221 gaggggggcg tcctccttgc ggagcagggg cgacccgacg taccggccca tctcgacgga
     2281 ggtgccggtc atcgtgccga cggcaccctc ctcggccgcg gcctcgccag gaagcggacc
     2341 ttgcgccatg cttcagccct cctcacgcgt cggccggagc gccgcgcatc gtgccggccg
     2401 cgctctggat cgccttgacg atgttgtggt agccggtgca gcggcagagg ttgccctcga
     2461 gcccgagccg gatctcccgg tcgctcatgc cggcaccgct gtcgacgagg ccgaccgccg
     2521 ccatgatcat cccgggcgtg cagtagccgc actgcagcgc gtggttctgg cggaacgcct
     2581 cctgcatggg gtggagctcg ccgttgcggg cgaggccctc gatggtggtg acctcggtgc
     2641 cgtcggcctg gacggcgagc atcgtgcagg acttgaccga ctcgccgtcc acgaggacgg
     2701 tgcacgcccc gcacgaggac gtgtcgcagc cgacgttcgt cccggtcagc ccgagcgtct
     2761 ctcgaaggaa gtagaccagc agctgtcgag gctcgacctc ggcctcgcgc cggaccccgt
     2821 tcaccgtcag agcgatgcgc ctcaagggcc acctccgtcc ctcgcccctc gccgaggggc
     2881 ctggtccggg gtccagcccg gtcccatctc cccagctccg gacgatgttg gccgccaatg
     2941 gtcggacgag ggcacaatag atccttct
//
LOCUS       DASPYY010000745         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2372511, whole genome shotgun sequence.
ACCESSION   DASPYY010000745 DASPYY010000000
VERSION     DASPYY010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372511"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VEP73_11665"
     CDS             <1..414
                     /locus_tag="VEP73_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYT27127.1"
                     /translation="LLRPGVRHLDGEAVDVGGLRVGFVGGGLPRSVRPHLSEVTEDAF
                     AAKVARLPRVDVLCTHVPPAVDDLRFDVLAGRFEPGSQALLDYVEEQQPDYLYFGHVH
                     QPRASRMRIGRTRLVNVGHFRATGRMLVHPVDGRP"
     gene            490..927
                     /locus_tag="VEP73_11670"
     CDS             490..927
                     /locus_tag="VEP73_11670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYT27128.1"
                     /translation="MPERAHGSTQVYGTAKEIMEVISDFEAYPEWAGQLKEVRVLERD
                     RRGRGTVVSFEVETPVFPAAYTLRYDYGPGDAGMSWSYVEGTLDDLAGSYTLEPLDDQ
                     STRVTYELLVGLNLPLPGLVKRQAARQIVKSALSDLKRRVESG"
     gene            1058..>1680
                     /locus_tag="VEP73_11675"
     CDS             1058..>1680
                     /locus_tag="VEP73_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HYT27129.1"
                     /translation="MRILLFTGKGGVGKTTVAAATAARAAVLGHRTLVMSTDPAHSLA
                     DSFDTELGAEPRQVATRLWAEQIDSQARLEANWREIQDYVVNLLNWGGVDSVEAEELS
                     VIPGLDELFSLIDINRYQQRGDYDLLVVDCAPTAETLRLLSLPEALGWYIDRLFPSSR
                     RIATVARPLLGRLGSLPPIASDAVFGAVERLYRNLERVRVLLAEPESS"
BASE COUNT          222 a          619 c          610 g          229 t
ORIGIN      
        1 ctcctccggc ccggcgtcag gcacctcgac ggcgaggcgg tcgacgtggg cgggctccgc
       61 gtcggcttcg tcggcggcgg cctgccgcgg agcgtccgcc cgcacctgtc ggaggtgacc
      121 gaggacgcgt tcgcggccaa ggtcgcccgc ctgccccggg tcgacgtgct ctgcacccac
      181 gtcccgcccg ccgtcgacga cctccggttc gacgtgctgg ccggcaggtt cgagccgggc
      241 agccaggcgc tgctcgacta cgtggaggag cagcagcccg actacctgta cttcggccac
      301 gtccaccagc cgcgggcgag ccgcatgcgc atcggccgca cccggctggt caacgtcggg
      361 cacttccggg cgaccggccg gatgctcgtg caccctgtcg acggccgccc atagcgaggg
      421 agcggccgcg ggggagcggc gcacggcgta ctaccatgag cctccatccg cctgtgccga
      481 ggagcggcga tgcccgagcg cgctcacggg tcgacccagg tctacgggac ggccaaggag
      541 atcatggagg tcatctcgga cttcgaggcc taccccgagt gggcgggcca gctcaaggag
      601 gtccgcgtgc tcgagcggga ccgccggggg cgggggaccg tcgtgtcctt cgaggtcgag
      661 accccggtgt tcccagccgc ctacaccctc cgctacgact acggccccgg tgacgccggc
      721 atgtcgtggt cgtacgtcga gggcacgctc gacgacctgg ccgggtcata caccctcgag
      781 ccgctcgacg accagtccac ccgcgtcacc tacgagttgc tggtcgggct gaacctcccc
      841 ctgcccggcc tggtgaagcg ccaggctgcc aggcagatcg tcaagtcggc cctgtccgac
      901 ctgaagcgcc gggtggagtc cggctgagcc ccggacgaga gcgcccggcg cgggtgccgc
      961 cggcgtccgg gaggcgacgg ctcagcgggc gctgcggacg cggcggacgc ggcgtaggct
     1021 gccggcgctc gggtagtcca cgacctcggg agcgtgcgtg cggatcctgt tgttcaccgg
     1081 caagggcggc gtcggcaaga cgacggtggc ggcggcgacg gcggccaggg cggccgtgct
     1141 cggccaccgc acgctggtga tgtcgaccga cccggcgcac tcgctggccg actccttcga
     1201 caccgagctc ggggccgagc ccaggcaggt cgcgacccgc ctgtgggccg agcagatcga
     1261 cagccaggcc cggctcgagg cgaactggcg cgagatccag gactacgtcg tcaacctgct
     1321 gaactggggc ggggtggaca gcgtcgaggc cgaggagctg tcggtcatcc ccggcctcga
     1381 cgagctgttc agcctcatcg acatcaaccg ctaccagcag cggggggact acgacctgct
     1441 ggtggtggac tgcgccccca ccgcggagac gctgcgcctg ctgtcgctcc ccgaggccct
     1501 cggctggtac atcgaccgcc tgttcccgtc ctcccggcgc atcgccacgg tcgcccggcc
     1561 gctgctcggc cggctcggca gcctgccccc gatcgcctcg gacgcggtct tcggagcggt
     1621 cgagcgcctg taccggaacc tcgagcgcgt ccgggtgctg ctcgccgagc ccgagtcgtc
//
LOCUS       DASPYY010000746         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2372651, whole genome shotgun sequence.
ACCESSION   DASPYY010000746 DASPYY010000000
VERSION     DASPYY010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372651"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /locus_tag="VEP73_11680"
     CDS             complement(<1..>1532)
                     /locus_tag="VEP73_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HYT27130.1"
                     /translation="VDDGFVDSDGDGLADCVDPDDDNDGSPDDADCAPLDPGAFLAPP
                     EEVRDLHWGESDGGRTLLFWTDQGSGMRYDLAGGTVSSLLADRSTLGAACIAADLAVP
                     QFDDARPDPPAGEAYYYVVRSGKIGCGFGTYGFESSGEERQLPNACPPAGPGRPDIDV
                     APASVDFGDVRAGQSASQQVTVRNVGSAALHVSGLTLSGAAAFNLVDAPATPLTLDSG
                     ARLVLTLSYGPSGPGTDSGTLEIDSDDGEEPTTLVPLAGRGVAPLLEVQPASIDFGPI
                     GVGIQADRTVSVRNAGNADLTLQAPSLAAGSSPFFSVGSPGSTLLPPTASTTFIVSFK
                     PGSEGPAAGSVLVGSDGGTFSLPLSGLGVTGATVVSLALNRAGLSLAQGDCFQLRAVA
                     TLSDATTQDVTQSALWSALDPAVASVGNGGLVLAMGRGGTTVSASFSGFSAAAAVDIV
                     DPGTLRLALPCGEFVSGSTFDAEVRIDAGPRPLGAYALLITYDPAVVSIGGIGGGSSA
                     PF"
BASE COUNT          225 a          533 c          578 g          196 t
ORIGIN      
        1 tgaacggggc cgagctgccg ccaccgatgc cgccgatgct gaccaccgcg gggtcgtagg
       61 tgatcagcag ggcgtaggcg cccagcggcc gcgggcccgc gtcgatccgc acctcggcgt
      121 cgaaggtcga tcccgacacg aactccccgc acggcagcgc caggcgcagc gtcccgggat
      181 cgacgatgtc gacggctgcg gcggctgaga aaccggagaa cgatgccgac accgtggtgc
      241 caccgcggcc catcgccagg accaggccgc cgttgccgac cgaggcgacg gcgggatcga
      301 gggcggacca gagggccgac tgcgtgacgt cctgcgtggt ggcgtccgac agcgtcgcga
      361 ccgccctcaa ttggaagcag tctccctgag ccaggctcag gccggcgcgg ttgagcgcca
      421 gggacacgac cgtcgcgccc gtcacgccga ggcccgacag cggcagcgag aacgtgccgc
      481 cgtcgctgcc gacgagtacg gagccggccg ctggcccttc gctcccgggc ttgaaggaga
      541 cgatgaaggt ggtggaggcg gtggggggca ggagcgtgct gccggggctg ccgaccgaga
      601 agaacgggct ggagccggcc gccagggacg gggcctgcag cgtgaggtcg gcgttgccgg
      661 cattccggac ggagacggtt cggtccgcct ggatcccgac gccgatcggc ccgaaatcga
      721 tcgaggcagg ctggacctca agaagaggcg ccacgccccg cccggccagc ggcacgagcg
      781 tcgtcggctc ctcgccgtcg tcgctgtcga tctcgagcgt cccggagtcc gtgccaggcc
      841 cggacgggcc gtacgacagg gtcaggacga gtctcgcgcc ggagtccagg gtcagaggcg
      901 tcgccggggc gtcgacgagg ttgaatgcgg cggcgccgct gagtgtcaat ccgctcacgt
      961 ggagcgcggc cgacccgacg ttgcggacgg tcacctgctg gctggcgctc tgtcccgccc
     1021 gcacgtcgcc gaagtcgacc gacgcgggcg ccacgtcgat gtcgggccgg ccggggccgg
     1081 cgggcgggca ggcgttcggc agctgccgct cctcgccgga cgactcgaac ccgtaggtgc
     1141 cgaacccgca gccgatcttt cccgaccgga cgacgtagta gtaggcctcg cccgcgggcg
     1201 ggtcggggcg cgcgtcgtcg aattgcggca ccgccagatc ggcggcgatg cacgcggcgc
     1261 cgagcgtgct tctgtcggcc aggagactcg acaccgttcc gcccgccagg tcgtagcgca
     1321 tcccggatcc ctggtcggtc cagaagagca gggtcctgcc gccgtcactt tcgccccagt
     1381 gcaggtcccg gacctcctcg ggcggagcca ggaatgcgcc ggggtcgagt ggggcgcagt
     1441 cggcgtcgtc gggagacccg tcgttgtcgt cgtcgggatc cacgcaatcg gccagaccgt
     1501 caccgtcgct gtcgacgaac ccgtcgtcga cg
//
LOCUS       DASPYY010000747         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1108248, whole genome shotgun sequence.
ACCESSION   DASPYY010000747 DASPYY010000000
VERSION     DASPYY010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108248"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VEP73_11685"
     CDS             complement(<1..1299)
                     /locus_tag="VEP73_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27131.1"
                     /translation="MPRTSSGHARRGAGRRELHDRRHRRPSEAAAPSWQRLLVVFLAL
                     GSLLGAGIGAVHAQSAPGASRRGDATTTTVAPQEPAPVSCRLRVPADPLSARGLATPW
                     VLAGDGRPCDETDPGQAAFVEATILDPATGDVTIYRPLVVNEGGDGGAAVRPQVPRLP
                     ARAVVGINVGFNGDSLTLDDTPGVTRGRCDNGIPGSIFGQVIFCNDAAFFAAAHRALA
                     AHLPGLAIPPLGTDLHGDVCPTTSSFDVVDQDAKDNSTTTYLAAGGRTAQATAANAAR
                     LAGATQLANGSDHRLFSRFIVPALGCDAPRTTDLPLAKDVTDPRHPVMATSMHLMDLS
                     ANVHADAIPAALIPAGNPMVLVDGEPNLEKLNRYRVDVDEPPVQSLDQASTAAFCEGL
                     LGADPAGRPLAPAAGLAKLFEDRDLTIGAPSPEPDVATNLF"
     gene            complement(1595..>1921)
                     /locus_tag="VEP73_11690"
     CDS             complement(1595..>1921)
                     /locus_tag="VEP73_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYT27132.1"
                     /translation="ADLGLLLVYWSEPGDETLPLGAAPTRLPGFASRAELVAAYAAAS
                     GRPVRQLDFFVALGYWKLAVILEGVYARYAAGAYGTGDDAYRAFPEVVARLGDLALLA
                     VERAGR"
BASE COUNT          239 a          640 c          809 g          233 t
ORIGIN      
        1 gaacaggttg gtggcgacgt cgggctcggg cgacggcgcg ccgatcgtca ggtccctgtc
       61 ctcgaacagc ttcgccagcc cggcggcggg cgccaggggc cggccggccg ggtcggcgcc
      121 gagcaggccc tcgcagaacg cggcggtgga cgcctggtcc aggctctgca cggggggctc
      181 gtcgacgtcg acgcggtagc ggttgagctt ctcgaggttc ggctcgccgt ccaccagcac
      241 catggggttg cccgccggga tcagggcggc cgggatggcg tcggcgtgca cgttggccga
      301 gaggtccatg aggtgcatgg aggtggccat gaccggatgg cgggggtcgg tgacgtcctt
      361 ggcgagcggc aggtcggtcg tcctgggggc gtcgcagccg agcgcgggca cgatgaaccg
      421 ggagaagagc cggtggtcgc tgccgttcgc gagctgggtg gcgccggcga gccgcgccgc
      481 gttggccgcc gtggcctgcg cggtcctgcc cccggcggcc aggtaggtgg tggtcgagtt
      541 gtccttggcg tcctggtcca cgacgtcgaa cgacgacgtg gtcgggcaga cgtcgccgtg
      601 caggtcggta ccgagcggcg ggatcgcgag gccggggagg tgggcggcga gcgcccggtg
      661 ggcggccgcg aagaacgccg cgtcgttgca gaagatcacc tggccgaaga tcgaccctgg
      721 gatcccgttg tcgcaccggc cacgcgtgac gcccggggtg tcgtcgaggg tgaggctgtc
      781 gccgttgaag ccgacgttga tgccgacgac ggcacgggcg gggagccgcg ggacctgggg
      841 ccggacggcg gcgcccccgt ccccgccctc gttgaccacc agcgggcggt agatggtgac
      901 gtccccggtc gccgggtcca ggatcgtcgc ctccacgaac gccgcctggc cggggtcggt
      961 ctcgtcgcac ggccgtccgt cgccggcgag cacccagggg gtcgcgaggc cgcgggcgga
     1021 gagcgggtcg gcgggcaccc ggagccggca gctgaccggg gccggctcct gcggggcgac
     1081 ggtggtggtc gtggcgtccc cgcggcgcga ggccccgggg gcgctctgcg cgtgcaccgc
     1141 gccgatgccg gccccgagca gggacccgag agcgaggaag acgaccagca gccgctgcca
     1201 ggatggcgcg gccgcctcgg agggccggcg gtggcgccgg tcgtgcagct cgcggcgacc
     1261 ggcaccgcgg cgagcgtgtc ccgaactggt tcgtggcatg gaaccgccac ctttctggta
     1321 cggcacgacg tcgccacgcg gcgtgaggca gcgcggggtc accgccgccc gagcggggag
     1381 gagaggtcgc atcgggcagg cccacgacac cgggaatggg ggtccggctc gggtcgccgt
     1441 cgtgcggtat ggggaggggg tatgggggac gggggatgcc atctggggga atggcgggtc
     1501 tgactgtacg gtaccgcttt ctactcgtca aagcggggca cggcacccgc ggacgccggc
     1561 tccgggcccg gctcggggcc gggcccgggc gcgctcagcg gcccgcgcgc tcgaccgcca
     1621 gcagggcgag gtcgcccagg cgggccacca cctcggggaa cgcccggtag gcgtcgtcgc
     1681 ccgtcccgta cgcgccggcc gcgtagcggg cgtacacgcc ctcgaggatg accgcgagct
     1741 tccagtaccc cagcgcgacg aagaagtcga gctggcgcac cgggcgtccg gaggcggcgg
     1801 cgtacgcggc gaccagctcg gcccgcgaag cgaacccggg caggcgggtc ggggcggcgc
     1861 cgagcgggag cgtctcgtcg cccggctcgc tccagtagac gagcaggaga ccgaggtcgg
     1921 c
//
LOCUS       DASPYY010000748         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_968662, whole genome shotgun sequence.
ACCESSION   DASPYY010000748 DASPYY010000000
VERSION     DASPYY010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968662"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VEP73_11695"
     CDS             <1..106
                     /locus_tag="VEP73_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYT27133.1"
                     /translation="VVDTYVHYLRRKLGRGVIRTMRGAGYRMGPMGSG"
     gene            139..1416
                     /locus_tag="VEP73_11700"
     CDS             139..1416
                     /locus_tag="VEP73_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT27134.1"
                     /translation="MLRHAQLVLALQTALAVAVVLALVGAVAVLFVVHDEGVSADRLL
                     AAATSPAARDDVTDPPPGVTLLLVGPDGGVLAATPGRPAGLLDRRGLAATPGHPRYTN
                     RSFDGGHYRVLTVAEQGQGQPQRRFVQAALDRGPQAAERRRLLGAALVAGLCGVVAAG
                     VVGALLARRAVAPLGEAIGRQRRFVADASHELRTPLTLLHTRAQLVRRDLAAGELDRL
                     GAEVAALERDAARMGEVVEDLLVSAELLHGHGEQLPVDLGALAAEVADAARGHAAAAG
                     VALVTELEPAWVQGAPAGLRRVVVSLLDNAISHAKRPGGEVRLRVTATASARQAVLEV
                     RDDGPGLDPSGAARTFERFAHGPTTDGRRRFGLGLALVREVVDAHGGQIEAAGQPGPD
                     HGAIFRVQLPAAPHPPDAGRAAPGAPGPGVRQR"
     gene            1451..2689
                     /locus_tag="VEP73_11705"
     CDS             1451..2689
                     /locus_tag="VEP73_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT27135.1"
                     /translation="MASMRSASTSGDPAGGAGSSGRAQAGHRPAVPAAVLWPLRAFLG
                     FSFVDAGLGKLLAPGWFGHGPQSLAVQARAYAHGSPLHGLISALVLPHPAVFALLIAA
                     AELAVGLLTLVGLASRGAAGVGLALSLSFFLTASWHTRPFFYGPDLPFAVGWLVLLQA
                     GHGGLPSVDRWLAERERAAAGLPAPDDRLLAVPAGRIEELCAERDPRGRCAAAAGRAC
                     RGAHCPLVPAAPGAAVVAASRRVFLQRAAAAGATLAGVGLLGGLAALVGTFLSPRDRS
                     VAPPPEALPARKPKSGPAGPPPTAANPGPVPQGVRIGNLADVPVGQAASFTDPHSGRP
                     AILVRLSSTRAVAYDAVCTHAGCTVGFDTASNVLACPCHGAEFDPAHGGAVIAGPAPT
                     PLPAIGLHIAPGGAVYVSGS"
     gene            complement(2708..3598)
                     /locus_tag="VEP73_11710"
     CDS             complement(2708..3598)
                     /locus_tag="VEP73_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HYT27136.1"
                     /translation="MSTPQSGIFALGTASHAYLELDARERGAARELVSAVASLREPRT
                     TMGGVNLVAGFRPELWRELMPDDAPPEVEGFNRELLGADGYVMPATQHDAVLWLSGSA
                     YDVIFDTAREAIASLGGLASVAEETSSWPYRHDRDLTGFIDGTENPSLVDAPQVALIP
                     DGDPGAGGAVLLLQKWAHDVAAWESLPAARQEQVMGRTKLDSVELEDKAPESHVGSTD
                     QDRFGKIFRRNMPYGTVTDHGTMFVGFSAEQRRLQAMLESMAGLTDGARDALTSYARA
                     LTGAYYFVPSTQSLRRCGCM"
     gene            3734..4402
                     /locus_tag="VEP73_11715"
     CDS             3734..4402
                     /locus_tag="VEP73_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27137.1"
                     /translation="MFDPIRMDAARRAVARDEERTRLANLVGPTALAADQLLPVLPPL
                     ARLLPHGGLRRGTVVTIDGSLRLALALAASVTQADRWAAAVGIPELGVVAAAEIGVVL
                     RRFPLVPDPDRRWAQVVAALLHAVDLVVARVPATGSPASCRQLATRVRERGSVLLALG
                     AWPAPDLVLSVSGETWTGLEAGAGRLTQRRVRVVATGRGAAALPREAWLWLPFRDGTV
                     QPAD"
BASE COUNT          523 a         1736 c         1716 g          633 t
ORIGIN      
        1 ggtggtcgac acctacgtcc actacctgcg tcgcaagctc ggccgcggcg tgatccgcac
       61 gatgcgcggc gctggctacc gcatgggacc gatggggtcg ggttgagccg gggtcgggcc
      121 gcggcggcgg agcgggcgat gctgcgccac gcccagctgg tgctggcgtt gcagacggcg
      181 ctcgcggtcg ctgtggtgct cgcgctggtc ggagcggtcg cggtgctgtt cgtggtgcac
      241 gacgaggggg tgtcggccga ccggctactg gccgcggcga ccagcccggc ggcccgggac
      301 gacgtcaccg acccgccacc gggcgtgacc ctgttgctgg tcggcccgga cggcggagtg
      361 ttggccgcga cgccgggcag gccggctggg ctgctggacc ggcgcgggct ggcggcgacg
      421 ccggggcatc cccgctacac caaccggtcg ttcgacggcg gccactaccg cgtcctcact
      481 gtcgccgagc agggccaagg ccagccccag cggcgctttg tgcaggcggc gctcgaccgt
      541 ggcccgcagg cggccgagcg tcgccggctg ctcggcgcgg cgctggtggc tgggctgtgc
      601 ggcgtcgtgg ccgcgggggt cgtgggggcg ctgctggcca ggcgggcggt cgcgccgctc
      661 ggcgaggcga tcgggcgcca gcgacgcttc gtggccgatg cctcccatga gctgcgcacc
      721 cccctgacac tgctgcacac ccgcgcgcag ctcgtgcgcc gcgacctggc ggccggtgag
      781 ctcgaccggc ttggcgcgga ggtggccgca ctggagcgcg acgccgcccg catgggcgag
      841 gtggtcgagg acctcctggt cagcgcggag ctgctccacg gccacggcga gcagctcccg
      901 gtggaccttg gcgcgctcgc cgcggaggtg gccgacgccg cccgcggcca cgccgcagcc
      961 gccggggtcg cgctggtgac cgagctcgag cccgcctggg tgcagggcgc cccggcgggg
     1021 ctgcggcggg tcgtggtgag cctgctcgac aatgccatca gtcacgcgaa gcggccgggc
     1081 ggcgaggtgc ggctgcgggt gaccgcaacg gcctcggcgc ggcaggccgt gctcgaggtc
     1141 cgcgacgacg gtccgggcct ggacccctcc ggtgccgcgc gtacgttcga gcgcttcgcg
     1201 cacggcccga ccaccgacgg ccggcgacgc ttcggtctcg ggctggcgct ggtccgcgag
     1261 gtcgtcgacg cccacggcgg ccagatcgag gccgccggcc aacccggtcc cgaccatggg
     1321 gccatcttcc gcgtgcagct gcccgccgcg ccacatcccc cggacgcagg ccgggcagcg
     1381 cccggagcgc cggggccagg tgtgcggcaa cgctaagacg ccgccaagaa tccgctggca
     1441 ggctatgggc atggcgagca tgcgatcggc gtccacgtcg ggggatcccg caggtggagc
     1501 tggcagctcc ggaagggccc aggccgggca ccgcccggcc gtcccggcgg cggtgctgtg
     1561 gccgctgcgg gcgttcctcg gcttcagctt cgtggacgcg gggctcggca agctgctcgc
     1621 gcccggctgg ttcggccacg gcccgcagag cctggccgtg caggctcgcg cctacgccca
     1681 cggcagcccg ctccacggcc tgatcagcgc gctggtgctg ccgcacccgg ctgtcttcgc
     1741 cttgctgatc gccgcggcgg agctggccgt cgggctgctc accctggtcg ggctcgcctc
     1801 cagaggggcg gccggggtgg ggctggcgct gtccctgtcg ttcttcctga ccgccagctg
     1861 gcacacccgg ccgttcttct acgggccgga tctgccgttc gcggtcggct ggctggtgct
     1921 gctgcaggca ggacacggcg gtctgccgag cgtggaccgc tggctcgccg aacgggagcg
     1981 ggccgccgcg ggcctgccgg cgccggacga tcggctgctc gcggtccccg ccggccggat
     2041 cgaggagctc tgcgccgagc gcgaccctcg cgggcgctgc gcggcggctg cggggcgcgc
     2101 ctgccgtggc gcccattgcc cgctggtgcc ggcggcaccc ggcgcagcgg tcgtggcggc
     2161 gtcgcgccgc gtcttcctgc aacgcgccgc tgccgccggc gcgaccctgg ccggtgtcgg
     2221 cctgcttggc gggctcgccg ccctggtcgg caccttcctg agcccgcgtg accgcagcgt
     2281 cgctccgccg cccgaagcct tgccggcgcg caagcccaag tccggacccg ccggcccacc
     2341 acccaccgcg gccaacccag ggccggtgcc gcagggtgtc cgcatcggca acctcgccga
     2401 cgtgccggtg ggccaggctg cctcgttcac cgacccgcac agcggacgtc ccgcgatcct
     2461 ggtgcggctc agctcgaccc gtgcggtcgc ctacgacgcg gtctgcaccc atgccggctg
     2521 cacggtcggc ttcgacacgg ccagcaacgt gctcgcctgc ccctgccacg gcgcggagtt
     2581 cgaccccgcc cacggcggcg cggtcatcgc cggccctgcc cccacaccgc tgcctgccat
     2641 cggcctgcac atcgccccag gcggcgccgt ctacgtcagc ggatcatgat gaccacgcgg
     2701 caccgcccta catgcagccg cagcggcgca agctctgcgt cgaggggacg aagtagtacg
     2761 cgccggtgag ggctcgagcg tagctcgtga gcgcgtctcg agcgccgtcg gtcaggccgg
     2821 ccatgctctc gagcatggcc tggagccgcc gctgttcggc actgaaaccg acgaacatgg
     2881 tgccgtggtc ggtcacggtg ccgtagggca tgttgcgccg gaagatcttg ccgaagcgat
     2941 cctggtcggt gctgccaacg tgggactcgg gcgctttgtc ctccagctcg acgctgtcga
     3001 gcttcgtgcg ccccatcacc tgctcctgcc gagcggcggg cagtgactcc cacgcggcca
     3061 cgtcgtgggc ccacttctgc aggagcaaga cggccccgcc ggctccggga tccccatcgg
     3121 ggatcagggc gacctgtggc gcgtcgacca ggctggggtt ctcggtcccg tcgatgaacc
     3181 cggtcaggtc gcggtcgtgg cggtagggcc agctcgacgt ctcttcggcc accgacgcga
     3241 gcccgccgag ggaggcgatc gcctcgcgcg ccgtgtcgaa gatcacgtca tacgcgctcc
     3301 cggaaagcca cagcacggca tcgtgctgcg tggcgggcat cacgtagccg tcggcaccga
     3361 gcagctcgcg gttgaagccc tcgacctctg ggggagcgtc gtcgggcatg agctcgcgcc
     3421 agagctcggg acggaagccc gcgaccaggt tcaccccgcc catcgtcgtt cggggctcgc
     3481 gcagcgacgc gaccgcgctc accagctccc gcgcggctcc tcgctcccgc gcgtcgagct
     3541 cgaggtaggc atgcgacgcc gtcccgaggg cgaagatccc gctctgcggc gtgctcatcc
     3601 ggcaagcatg aaccggcgcg ctcggatcgt ctaccacggc cgtccctgct caggagcctg
     3661 ttcggcattc gtcccgaagg ctctcgatcc ccaacaattc cggaccggcc tgtaagttgc
     3721 aaggatcgaa caagtgttcg atcctatccg catggatgcg gcacgccgcg cggtggcccg
     3781 cgacgaggag cgcacccgcc tggccaacct cgtcgggccg acggcgctcg ccgccgacca
     3841 gctcctgcct gtgctgccgc cgctggccag gctcctgccg cacggcgggc tccgccgcgg
     3901 cacggtggtc acgatcgacg ggtccctgcg gctcgcgctg gcgctcgcgg ccagcgtcac
     3961 ccaggccgac cgctgggccg ccgccgtcgg catcccggag ctcggcgtgg tcgccgccgc
     4021 cgagatcggc gtcgtgctgc ggcggttccc tctcgtcccc gacccggacc ggcggtgggc
     4081 acaggtggtg gccgccctgc tgcacgcggt cgacctggtg gtcgcccggg tccccgccac
     4141 cggaagcccg gcctcgtgcc gccagctcgc cacccgggtc cgcgagcgcg gcagcgtcct
     4201 gctcgctctc ggcgcctggc cagcccctga cctggtcctc agcgtcagcg gcgagacgtg
     4261 gacggggctg gaggccggag ccgggcgcct cacgcagcgg cgggtgcggg tggtcgccac
     4321 cggccggggc gccgccgccc tcccccgcga ggcgtggctc tggctgccct tccgggacgg
     4381 gaccgtgcag ccggccgact gacgcgcccg gccgccctcc gcgggctttg ctcgggggcc
     4441 atccgtgccg agcatcatcg ggagacgcaa ggccgtcggg cgagaagcaa gctggacgaa
     4501 cacacgttcg ccccattgta atgggcgggc acgccgccca cgacgctagt tggcatcctc
     4561 ccccgccgaa ggcgggggat tgctccacgc cgcgaggagg cgcaggtg
//
LOCUS       DASPYY010000749         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2373932, whole genome shotgun sequence.
ACCESSION   DASPYY010000749 DASPYY010000000
VERSION     DASPYY010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2373932"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..>2194)
                     /locus_tag="VEP73_11720"
     CDS             complement(30..>2194)
                     /locus_tag="VEP73_11720"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT27138.1"
                     /translation="ADQPVAIVAVVRAAAGDPSPFEPISQLPHLTELRVTEMASADAE
                     RLVSQRLRSRYGAEALLAPGVVRRIADRGEGNPFYLEELVSFLHAQGIDPRDPDALAT
                     LDLPDGLHRLVMARIDRLSEGEKATIKVASVIGRQFRAGWISASYPAAGSRREVARHL
                     ERLGDLDLTPIRTVGPEPEYAFKHAIIQEVAYQSLTYQMREVLHEQVGLYIEAAYPDR
                     LVQYVDVLAHHYGRTRRVDKQRVWFRVAGDAAKAAFANEAAIAYFSALLPLLPEPETA
                     EVLVELGTVWQLTGKWREAERAYRRALRIAELTGDRTVLAASQRQLGDLSMYTESYTE
                     AVRWLRQAAGEFEGLGDRQGLSGVLDRLAYTYIHQSAYGEALATAERHLAIATEADDP
                     AGVSVALNHMSLVRWHNGETTSALSLLRRSLDAATGAGDRRCEIHAANTLACVHFDRG
                     DHQDAVASWRRALAMAGAIGYRQTAAVIVGNLGEVYLLHGDHRLATSCFAHALRVAAE
                     LGDWTSTAYRVASLATAAAAEGREEDAARMLSRAAALSRMLDASRYLCFCLHHQARLL
                     EARGRLLEAEQLNQEALEVATRDNEHAIQVSARLLALRLQVALGRLDRDAAVRQLRDV
                     QATWTEPHERAALLDTTWQLDPAQEAARVEAARLYRTLYGRAPIVEYRDAYERLTGVR
                     LPPGPPLPPLPDAVADEAVDIEAVLRQADLATRQLGAA"
BASE COUNT          271 a          789 c          833 g          301 t
ORIGIN      
        1 gttcgcggtg ggggcgccgt gacggtgcct cacgcggcgc cgagctggcg ggtggccagg
       61 tcggcctggc gcagcaccgc ctcgatgtcg acggcctcgt ccgcgaccgc gtcgggcagc
      121 ggcgggagcg gcggtccggg cggcagccgc acgccggtca gccgctcgta cgcgtcccgg
      181 tactcgacga tcggcgcccg cccgtagagc gtgcggtaca ggcgggcggc ctccacgcgg
      241 gctgcctcct gggccgggtc gagctgccag gtcgtgtcga gcagcgccgc ccgctcgtgc
      301 ggctcggtcc aggtcgcctg cacgtcccgc aactgccgca cggccgcatc ccggtccagc
      361 ctcccgagcg cgacctgcag ccgcagggcc agcaggcggg cgctcacctg gatggcgtgc
      421 tcgttgtccc gcgtcgcgac ctcgagcgcc tcttggttca gctgctcggc ctccaggagc
      481 cgcccccgcg cttcgagcag ccgcgcctgg tggtgcaggc agaagcagag gtagcgggag
      541 gcgtcgagca tccgggagag cgcggccgcc cgggagagca tccgcgcggc atcctcctca
      601 cgcccctcgg cggccgcggc cgtggccagg ctcgccaccc ggtacgccgt gctcgtccag
      661 tcccccagct cggcggcgac ccggagcgcg tgcgcgaagc agctggtcgc gaggcgatgg
      721 tcaccgtgga ggaggtagac ctcgcccagg ttcccgacga tgacggccgc ggtctgccgg
      781 tagccgatcg cgcccgccat cgccagcgcc cgccgccagg acgcgaccgc gtcctgatgg
      841 tcgccgcgat cgaagtgcac gcaggcgagc gtgttggccg catggatctc gcagcgccgg
      901 tcgcccgccc cggtcgccgc gtcgagcgag cgacgcagca gcgagagcgc cgacgtggtc
      961 tccccgttgt gccagcgtac gaggctcatg tggttgaggg cgacgctcac gccggcgggg
     1021 tcgtcggctt cggtcgcgat cgcgaggtgc cgctcggccg tggcgagcgc ctcgccgtag
     1081 gcgctctggt ggatgtaggt gtacgcgagg cggtcgagca ccccggacag cccctgccgg
     1141 tccccgagcc cctcgaactc gccggcggcc tgccgcagcc agcggaccgc ctccgtgtac
     1201 gactcggtgt acatggagag gtcgccgagc tgccgctggc tcgccgcgag gaccgtgcgg
     1261 tcgcccgtca gctcggcgat gcgcaacgcc cggcggtatg cccgctcggc ctcccgccac
     1321 ttgccggtca gctgccacac cgtccccagc tcgacgagca cctcggcggt ctcgggctcc
     1381 gggagcagcg gcagcagggc gctgaagtac gcgatggcgg cctcgttggc gaaggccgcc
     1441 ttggccgcgt caccggcgac gcggaaccac acccgctgct tgtcgacccg ccgggtgcgg
     1501 ccgtagtggt gggcgagcac gtcgacgtac tggacgagcc ggtccgggta ggccgcttcg
     1561 atatagaggc cgacctgctc gtgcagcacc tcccgcatct ggtacgtgag gctctggtag
     1621 gcgacctcct ggatgatcgc gtgcttgaag gcgtactcgg gctcgggccc gaccgtgcgg
     1681 atcggcgtca gatcgaggtc gccgaggcgc tcgaggtgcc gggcgacctc gcggcgcgac
     1741 cccgcggccg ggtagcttgc cgagatccac ccggcccgga actgccggcc gatgacgctg
     1801 gcgaccttga tggtggcctt ctcgccctcg ctgagccggt cgatccgggc catgaccagg
     1861 cggtggaggc cgtcgggcag gtcaagggtg gccaaggcgt ccgggtcacg cgggtcgatg
     1921 ccctgggcat ggaggaagct gacgagctcc tcgaggtaga acgggttgcc ttcgccgcgg
     1981 tcggcgatcc gacgcaccac cccgggggcc aggagggcct cggcgccgta gcggctgcgg
     2041 agccgctggc tgaccagccg ctccgcgtcc gcggaggcca tctcggtcac gcggagctcg
     2101 gtgaggtggg ggagttggga gatcggttcg aacggcgacg gatcgcccgc ggccgcgcgc
     2161 acgacggcaa cgatggccac gggctggtcg gccg
//
LOCUS       DASPYY010000750         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_266238, whole genome shotgun sequence.
ACCESSION   DASPYY010000750 DASPYY010000000
VERSION     DASPYY010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266238"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="meaB"
                     /locus_tag="VEP73_11725"
     CDS             complement(<1..794)
                     /gene="meaB"
                     /locus_tag="VEP73_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HYT27139.1"
                     /translation="MEEAPPAGGPGREVASLVERLRSGDRRAVARLISLVEDGARDRL
                     REVAETLNPSTGRAMVIGLTGAPGVGKSTLANALVTSYRAAGRTVGVLAVDPSSPFTG
                     GALLGDRVRMQHHALDQGVYIRSMATRGHLGGLAWATPQAVRVLDAAGCEVVLVETVG
                     VGQAEVEVAGLADTTLVVLAPGFGDAVQVAKAGILEVADVFVVNKADKDGADTVARDL
                     RQMLHLGDERPWGVPVVKTAAERRQDVDELVERIAAHRAHLEAGGGL"
     gene            complement(797..1987)
                     /locus_tag="VEP73_11730"
     CDS             complement(797..1987)
                     /locus_tag="VEP73_11730"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYT27140.1"
                     /translation="MFDRTEPVIVAGARTPIGRFGGALAPLEAVDLGGLAIAAALGRA
                     GVAPDAVEHVVMGHVLQAGQGQITARQAAVRAGIPMTVPALTVNKVCLSGLNAIATAA
                     QYVELGQAGLLVAGGMESMTNAPYLVPGARFGVRMGNGELVDAMVHDGLWDAFDHVHM
                     GDVSDQVNARLGIDRARQDEWAARSHERAAAAAKGALREEIEPVGVPQRKGDPVLVEQ
                     DEGVRPGTTAEALGGLRPAFTPDGSITAGNASQISDGAAAVVVTSRERAEADGLPVLA
                     RIVGYGSVAGPDNTLHSQPSRAVRDALRRGGLDVRDLDLLEINEAFAAVALRSTAELE
                     VDEAIVNVNGGAVALGHPIGASGARLVLTLALELGRRGGGTGAAALCGGGGQGDALLL
                     HVGE"
     gene            2122..2556
                     /gene="mce"
                     /locus_tag="VEP73_11735"
     CDS             2122..2556
                     /gene="mce"
                     /locus_tag="VEP73_11735"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HYT27141.1"
                     /translation="MQVGRLDHVGIAVADLDTARALYERAFGLEVAHEEVIQDQGVHE
                     LLFRLGDGWLQLVAPLGPDTPVGRFLARRGEGVHHIGYAVPDVARAIDDLSTAGLELV
                     DDAPRTGSGGTTIAFVHPKSTRGVLIELVEEGSGHRGKLATG"
     gene            2553..3818
                     /locus_tag="VEP73_11740"
     CDS             2553..3818
                     /locus_tag="VEP73_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYT27142.1"
                     /translation="MSTRAEPERKMREQAGAGGQGQPAPPPDEPAEEQVRDLGAAMPA
                     TPAQPDAIGARIRHGALFRWGFFAGLGVMAALAAAAAVWSVRDILLRVVVAAFLAVSL
                     DPAVRGLTARGMRRGMAVGLIFAVFVAIVAAFLMSVIPPLSKQIGELVHNLPHYLGSL
                     QQKSGRFRELNSRFDLSTRLEGLVSQLPGKLTGGVLGLTSQVFGALISFLTVVVFTVY
                     FLLDLPRLRRGVVRLFPVDRRTRYAAMVDIVVDKVGDYMIGRLLIAFVGGLVAFIGLE
                     VLHVPYPLPLAILIGLLDLIPLIGHPIGAAVGVVVSLFSRPIWPTTVVLVVFFLVYQQ
                     VENYLIGPRILRHSVDISAVAILLAALIGASILGVIGALVAIPVAAAVKVILVQQIDQ
                     HEAAHAATDDRPHRRLRRRHQGGDGQPGP"
     gene            complement(4063..>4459)
                     /locus_tag="VEP73_11745"
     CDS             complement(4063..>4459)
                     /locus_tag="VEP73_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27143.1"
                     /translation="DPAGVRVLGSLGRAYVSVSNFDVLPVVGVWDGRAPLRPSPAEVA
                     GLLLPTLRQLADPANHELVPLTRLLPPAALRARRIPPGVVSPVFWVDGIAVWGFTAGL
                     LSGFLGLLGLPAPELPAEWSRPVPRPPGR"
BASE COUNT          556 a         1672 c         1635 g          596 t
ORIGIN      
        1 agcccgccgc ccgcctccag gtgggcgcgg tgcgcggcga tgcgctccac cagctcgtcg
       61 acgtcctggc ggcgctcggc ggccgtcttg accaccggca cgccccaggg tcgctcgtcg
      121 ccgaggtgga gcatctggcg caggtcgcgc gcgaccgtgt cggccccgtc cttgtcggcc
      181 ttgttgacga cgaagacgtc ggccacctcg aggatgccgg ccttcgcgac ctggaccgcg
      241 tcgccgaagc cgggcgcgag cacgacgagg gtcgtgtcgg ccaggccggc cacctccacc
      301 tcggcctgcc ccaccccgac ggtctcgacc aggacgacct cgcacccggc cgcgtcgagc
      361 acccgcacgg cctggggcgt cgcccaggcg aggccgccca ggtggccccg cgtcgccatc
      421 gagcggatgt agacgccctg gtcgagggca tggtgctgca tgcgcacgcg gtcgccgagc
      481 agggccccgc cggtgaacgg cgacgagggg tcgacggcga gcaccccgac ggtgcggccg
      541 gcggcccgat acgaggtcac cagagcgttg gcgagggtcg acttgcccac cccgggtgcc
      601 ccggtcagcc cgatcaccat cgcccggccg gtcgaggggt tgagggtctc ggcgacctcc
      661 ctgagccggt cgcgggcgcc gtcctcgacg agcgagatga gccgggccac ggctcgccgg
      721 tcgccggacc gcagccgctc cacgagcgac gcgacctccc ggcccggccc gcccgccggc
      781 ggcgcctcct ccacggtcac tcccccacgt ggaggagcag ggcgtcgccc tggccgcccc
      841 cgccgcagag ggcggcggcg cccgtcccgc cgccccgcct gccgagctcg agggcgaggg
      901 tgagcacgag ccgggcgccg ctcgcgccga tcgggtggcc gagggcgacc gcgccgccgt
      961 tcacgttgac gatcgcctcg tcgacctcca gctcggcggt ggagcgcagg gcgaccgcgg
     1021 cgaaggcctc gttgatctcg aggaggtcga ggtcccggac gtccaggcca ccgcggcgca
     1081 gcgcgtcccg gaccgccctg gacggctgcg agtgcagcgt gttgtccggg ccggccacgc
     1141 tgccgtagcc gacgatcctg gcgaggacgg gcaggccgtc ggcctccgcc cgctcccgcg
     1201 aggtgaccac cacggcggcc gcgccgtcgg agatctgcga ggcgttgccc gcggtgatgc
     1261 tgccgtccgg ggtgaacgcg ggccgcagac cgccgagggc ctcggcggtc gtcccggggc
     1321 ggacaccctc gtcctgctcg acgagcacgg ggtcgccctt gcgctggggg acgcccaccg
     1381 gctcgatctc ctcccggagg gcgcccttgg cggccgccgc cgcccgctcg tgcgagcggg
     1441 ccgcccactc gtcctgcctg gcgcggtcga tgccgagcct cgcgttcacc tggtcggaca
     1501 cgtcgcccat gtggacgtgg tcgaaggcgt cccagagccc gtcgtggacc atcgcgtcga
     1561 ccagctcgcc gttgcccatg cgcacgccga agcgcgcccc tggcacgagg tacggcgcgt
     1621 tcgtcatcga ctccatcccg ccggccacga ggaggccggc ctggccgagc tcgacgtact
     1681 gggcggcggt ggcgatcgcg ttgaggccgg agaggcagac cttgttgacg gtgagcgccg
     1741 ggaccgtcat ggggatcccg gcccgcacgg ccgcctgcct cgcggtgatc tgcccctgcc
     1801 ctgcctgcag cacgtgaccc atgacgacgt gctcgaccgc gtcgggcgcg acgcccgcgc
     1861 ggcccagcgc cgccgcgatc gcgaggccgc cgaggtcgac tgcctcgagc ggcgccaggg
     1921 caccgccgaa ccggccgatc ggggtgcggg cgcccgcgac gatgacgggc tcggtacggt
     1981 cgaacattcg gtcccccatg gtcgtccctc gccggccgcg gacacgcgcg gcacgggcct
     2041 cgccgccccg gatcctcccg agtctaccgc cggcagggcg taggctctcc gggcccggcg
     2101 gagcgacgaa gggaccttgc cttgcaggtt ggacgcctgg accacgtcgg catcgcggtc
     2161 gccgacctgg acaccgcccg cgcgctctac gagcgcgcct tcggcctgga ggtggcgcac
     2221 gaggaggtga tccaggacca gggcgtccac gagctgctct tccgcctggg cgacgggtgg
     2281 ctccagctgg tggcgccgct cgggccggac acgccggtcg ggcgatttct cgcccggcga
     2341 ggtgaaggcg tgcaccacat cgggtatgcc gtccctgacg tcgcccgtgc catcgacgac
     2401 ctttccaccg cggggctgga gctcgtcgac gacgccccgc gcaccggctc gggcggcacg
     2461 accatcgcgt tcgtccaccc gaagagcacg cggggcgtgc tgatcgagct ggtcgaggaa
     2521 ggaagcggcc accgcgggaa gctggcgaca ggatgagcac gcgagcggag ccggagcgca
     2581 agatgcggga gcaggccggc gcgggcggtc agggccagcc ggcgccgccg cccgacgagc
     2641 ccgccgagga gcaggtccgc gacctcggcg ccgcgatgcc ggccaccccg gcacagccgg
     2701 acgccatcgg ggcgcgtatc aggcacgggg cgctgttccg ctgggggttc ttcgccggtc
     2761 tcggggtgat ggccgcgctc gcggccgcgg ccgcggtgtg gtcggtgcgc gacatccttc
     2821 tccgggtcgt ggtggcggcg ttcctcgccg tcagcctcga cccggccgtc cgtgggctga
     2881 cggcccgcgg gatgcgccgc gggatggccg tgggactgat cttcgccgtc ttcgtggcca
     2941 tcgtggcggc ctttctgatg tcggtcatcc cgccgctctc caagcagatc ggcgagctcg
     3001 tccacaacct gccccactac ctcggctccc tgcagcagaa gtcgggccgg ttcagggagc
     3061 tgaacagccg cttcgatctc agcacccggc tcgaggggct ggtgagccag ctccccggca
     3121 agctcaccgg aggggtgctc gggctcacga gccaggtatt cggggcgctg atctccttcc
     3181 tcaccgtcgt ggtcttcacc gtgtacttcc tgctcgacct gccccgcctg cgccggggcg
     3241 tcgtccgcct gttcccggtg gaccggcgca cgcgctacgc ggccatggtc gacatcgtcg
     3301 tcgacaaggt cggcgactac atgatcggcc ggctgctgat cgcgttcgtg ggcggcctgg
     3361 tggcgttcat cggcctcgag gtgctgcacg tcccctaccc gctgccgctc gccatcctca
     3421 tcgggctcct cgacctgatc ccgctcatcg gccacccgat cggcgccgcc gtcggcgtgg
     3481 tcgtgtcgct gttctcgagg cccatctggc cgaccacggt cgtgctggtc gtcttcttcc
     3541 tggtgtacca gcaggtggag aactatctca tcggaccccg catcctgcgt cactcggtgg
     3601 acatctcggc ggtcgcgatc ctgctggccg cgctcatcgg cgcgagcatc ctcggggtga
     3661 tcggggcgct cgtggcgatc ccggtcgcgg ccgcggtcaa ggtgatcctg gtccagcaga
     3721 tcgaccagca cgaagcggcc cacgcggcaa cggacgaccg gccgcaccgg cggctgcggc
     3781 ggcggcacca gggcggggat ggccagcccg gcccgtgacg gcgtggccgt tcgagaagag
     3841 agcgaggggg gcaggggccg gtcctcccct gcccccctcg cgtctgcagg ctaacccggc
     3901 gggggcacct cccgggcgcc acaacgggcc gagagtacta gatggatcgg ggcgttgtca
     3961 tcgccttcca ggtctcaaat ccataatggt gccacgaaat cgagcggcgc cccgttgatc
     4021 tgctcgggtt gctccaggcc ggacgccgcc ccatggccgc cgctaccgtc ccggcgggcg
     4081 gggcaccggc cggctccatt ccgcgggcag ttcgggggcg ggcaggccca gcaggcccag
     4141 gaatcccgag agcagcccgg cggtgaaccc ccacaccgcg atgccgtcga cccagaacac
     4201 cgggctgacc acgcccggcg ggatccggcg ggcgcggagc gcggcgggcg gcagcagccg
     4261 ggtcagcggg accagctcgt ggttggccgg atcggcgagc tgccgcaagg ttggcaggag
     4321 cagcccggcc acctcggccg ggctgggacg cagcggcgca cggccgtccc agacccccac
     4381 cacgggcagc acgtcgaagt tggacacgga gacgtaggcc cggccgaggc tcccgaggac
     4441 ccgcacgccg gccgggtcg
//
LOCUS       DASPYY010000751         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_550122, whole genome shotgun sequence.
ACCESSION   DASPYY010000751 DASPYY010000000
VERSION     DASPYY010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550122"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VEP73_11750"
     CDS             complement(<1..1039)
                     /locus_tag="VEP73_11750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYT27144.1"
                     /translation="MLSRADALETLGGERFDVVVIGGGITGAGVALDAASRGYSVALV
                     ERNDYASGTSSRSSKMIHGGLRYLQNFDLGLVREALLERQLMVELAPHLVYPIPFLVP
                     TLGEERRNWRIGIGLNMYDVMATTRVGQGRRERQARERASRERALLEPAEDDYWAPDR
                     HRTIPGEEAAELIPALASRDPKEAYLFYDCQTDDARLVLTVLGEAERFGAVFSNGAEV
                     VELISDGARAAGVVCADADSGERFEVRGANVVNATGVWADRIRPEEILAEEEVPRIAP
                     SRGTHITIPAEKLPVTQAACIVPAGEERTIFALPWYGRVLIGTTDRDYHGDIASVQPS
                     GEDVEYLLEAVN"
     gene            complement(1043..1552)
                     /locus_tag="VEP73_11755"
     CDS             complement(1043..1552)
                     /locus_tag="VEP73_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HYT27145.1"
                     /translation="MDVETAIRTRRTHKAFRPDQVPREVLGELFDLARWAPNHNLTNP
                     WRFRVLGPRALERLKLAAGPEAAPKLDRAPTLVVCSCVLGGDPVQDEEDLHATACAAY
                     IVLLAAHARGLAGYWRTPEVLRTEAGRTAVGLSKDERFVSLIHLGQPIQGKEAPERAA
                     VADTVTYLP"
     gene            complement(1596..>2558)
                     /locus_tag="VEP73_11760"
     CDS             complement(1596..>2558)
                     /locus_tag="VEP73_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27146.1"
                     /translation="RTTLALRGAGVSPHGFGGRDLVVELRKRRSRRGSFEGQVNLTAF
                     GILALRAAGTPASALARSAAWLRKAENGDGGWGFQPRAASDPDSTGAALQGLAAAGGR
                     GRATRRGASYLRRAQHADGGFALAAGGPTNSQSTAWAVQGLIAAGTNPAAVRTNGRNA
                     FDYLAKRQAGDGHYRYSSSSDQTPVWVTGQALVAVNREAFPLAAVPRAPAGRRSSPPG
                     APKSGTGASSGGGGSVASGTRAAVGGSQAASGRDSGKPRGARTRNGVRGELASGEPVS
                     APVAPSGAPADAGGLGTLAYVGGGFALLVAALAAGFFWFRRHLP"
BASE COUNT          347 a          936 c          936 g          339 t
ORIGIN      
        1 agttcaccgc ctcgagcaga tattcgacgt cctcgccgga tggctggacg ctcgcgatgt
       61 cgccgtggta gtcccggtcc gtggtgccga tcagcacgcg gccgtaccag ggaagcgcga
      121 agatcgtgcg ctcctcgccg gccggcacga tgcatgcggc ctgggtcacg ggaagcttct
      181 cggccgggat cgtgatgtgt gtgccgcggc tcggcgcgat ccgcgggacc tcctcctcgg
      241 cgaggatctc ctcaggtcgg atgcgatcgg cccagactcc ggtcgcgttg accacgttgg
      301 ccccccggac ctcgaagcgc tcgccgctgt cggcgtccgc gcagaccacg ccggcggccc
      361 tggcaccgtc ggagatcagc tcgaccacct cggcgccatt ggagaacacc gcgccgaagc
      421 gctccgcctc cccgagcacg gtgagcacca gccgggcatc gtcggtctgg cagtcgtaga
      481 acaggtaggc ctccttgggg tcgcgtgagg ccagcgccgg gatcagctcg gccgcctcct
      541 cacccgggat cgtgcggtgg cggtcgggcg cccagtagtc gtcctcggcc ggctccagca
      601 aggcgcgctc cctcgaggcg cgctcccgag cttggcgctc gcgccgcccc tgcccgaccc
      661 gggtcgtcgc catcacgtcg tacatgttca gcccgatccc gatccgccaa ttacggcgtt
      721 cctcgcccag ggtggggacg aggaacggga tcggatagac cagatgtggc gccaactcca
      781 ccatcaactg gcgctccagg agcgcctcgc ggaccaggcc gaggtcgaag ttctgcaggt
      841 agcgaagccc gccgtggatc atcttcgagg agcggctgga ggtgcccgag gcgtagtcgt
      901 tgcgctcgac cagcgccacc gagtagccgc gcgaggcggc gtccagcgcc accccggccc
      961 cggtgatccc gccgccgatc acgaccacgt cgaagcgttc gccgccgagt gtctcgagag
     1021 cgtcggcgcg cgagagcatc cgctatggga ggtaggtgac ggtgtcggcg acggcggcgc
     1081 gctcgggcgc ctccttgccc tggatcggct ggccgaggtg gatgaggctg acgaagcgct
     1141 cgtcctttga caacccgacc gcggtgcgcc cggcctcggt gcgcagcacc tccggcgtcc
     1201 gccagtagcc ggccagcccg cgggcgtgcg cggccagcag cacgatgtag gcggcgcagg
     1261 cggttgcgtg gaggtcctcc tcatcctgaa ccggatcgcc gccgaggacg cacgagcaga
     1321 cgaccagggt cggcgcccgg tcgagcttcg gcgccgcctc ggggccggcg gccagcttga
     1381 ggcgctccag cgcccgcggg ccgagcaccc ggaagcgcca cgggttggtc aggttgtggt
     1441 tcggcgccca gcgggcgagg tcgaacagct cgcccaggac ttccctcggc acctggtccg
     1501 gccggaaggc cttgtgggtg cgccgggtgc gaatcgcggt ctcgacatcc atgacgaaca
     1561 ccgtacccag tgaggttcgc tcgggctagg gctgttcacg gaagatggcg gcggaaccag
     1621 aagaagccag ccgccagggc agcgacgagc agggcgaagc cgccgccaac gtaggccagg
     1681 gtgccgagcc cgccggcgtc cgcaggggcg ccggacgggg cgacgggcgc gctcaccggc
     1741 tcaccggagg caagctcgcc gcgcacgccg ttacgggtcc gcgcgcccct cggcttgccc
     1801 gagtcgcggc cgctcgcggc ctggctcccg ccgaccgctg cacgggttcc gctcgccacc
     1861 gagccgccgc cacccgagct tgcgcccgtc ccactcttcg gcgcccccgg tggcgaggac
     1921 cggcgtccgg cgggcgcccg cggcacggcg gcgagcggga acgcctcgcg attgacggca
     1981 acgagcgcct gaccggtgac ccagaccggc gtctggtcgc tcgaggacga gtagcggtag
     2041 tggccatcac cggcctggcg cttggcgaga tagtcgaacg cgttgcggcc gttggtgcgc
     2101 acggcggcgg gattggtccc ggcggcgatc aggccctgta ccgcccaggc ggtcgactgt
     2161 gagttggtgg gtccgcccgc ggccagcgcg aagccgccgt cggcgtgctg agcgcgccgc
     2221 aggtaggacg cgccacggcg ggtcgcccga ccccggcccc ctgccgccgc caggccctgg
     2281 agcgcggcgc cggtgctgtc cgggtcgctg gcggcccggg gctggaagcc ccaaccgccg
     2341 tcgccgttct cggccttgcg aagccaggcg gccgagcgag cgagcgcgga cgcaggggtg
     2401 ccggcggcgc gcagggccag gatcccgaac gcggtcaggt tcacctggcc ctcgaacgag
     2461 ccgcgtcgcg agcgccgctt gcgcaactcc accaccaggt cgcggccgcc aaaaccgtgc
     2521 gggctcaccc ccgcccctcg cagcgcgagc gtggtccg
//
LOCUS       DASPYY010000752         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2092384, whole genome shotgun sequence.
ACCESSION   DASPYY010000752 DASPYY010000000
VERSION     DASPYY010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092384"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VEP73_11765"
     CDS             <1..996
                     /locus_tag="VEP73_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYT27147.1"
                     /translation="TPAQRTQAAQAALRAQRAADAASARQEGDSRDCADRQRAAAAQV
                     ATAQAAVAQASRSSSTPVAASVTSTTSAAGLAATLAANDAAVAQAQAAVDQAQAAVDA
                     TVLHAPVDGLVQVVNVVEDAVPPSGAAIQERSDTLTVQANVAEQDVTQIKGGQKAQVT
                     LPALGTTESATVAGLPSQANAAPSSASAAASTAVTFPVTLTFDQSDPAILPGMSAQLE
                     IALVRHANVLQVPTTAVQGGANGSTVQVLQDGRPVTRPVEVGLSTETATEIVVGLREG
                     QTVVTGVVNPTQGATQPTTGGGGFGGGGGARGGGFGGGGGLGGGGGGGARGGGGG"
     gene            1064..1762
                     /locus_tag="VEP73_11770"
     CDS             1064..1762
                     /locus_tag="VEP73_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT27148.1"
                     /translation="MEAWELARAYPFGQDAVVALRGVTLTVWPNESVAIMGASGSGKS
                     TLLHLLGLLDRPTSGTYRLAGVDTATLSDDRLAELRNRAIGFVFQSFNLVAGESAVEN
                     VAAPLVYQGLRRPERLRRAGEALERVGLGARMRHDPAQLSGGERQRVAIARALVTRPA
                     LLLADEPTGNLDSASGGELLALIDELHGAGLTVVVVTHDPAVAARAQRVVRVADGRVV
                     GEEAGPVTAGWRNR"
     gene            1759..>2711
                     /locus_tag="VEP73_11775"
     CDS             1759..>2711
                     /locus_tag="VEP73_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT27149.1"
                     /translation="MSLAEAFRLALRRLRANKLRTGLTTLGIVIGAASLVALLAVGQG
                     AQARLTSRIASLGTNLLSIQAGSSIGQGGIRGGLGSASTLTVDDADALRKLPGVAAVA
                     PELPVPGALVVAGRANTTTQVTGTTPDEARVRGYEVHAGTFLSDLELRAGLRVAVLGA
                     TTAADLGLPAKQAVGQTIQVDAIPFRVIGVTQPKGGFGNPDDQVFVPLSAVQRRLSGS
                     SSLRAIGLSVARADDMAAVSQQAEQTLRARHRLGDSASDDFLIANQAQLLQVAADQAT
                     VLRNFLLGIAAIALVVGGIGIANIMLVAVRERTREIGIRKA"
BASE COUNT          336 a         1002 c         1048 g          325 t
ORIGIN      
        1 accccggcgc agcgcacgca ggccgcccag gcggcgctgc gggcgcagcg cgccgccgac
       61 gcggcaagcg cccgccagga gggggacagc cgcgactgcg ccgaccgcca gcgggccgcg
      121 gcggcgcagg tcgccacggc acaggccgcg gtcgcgcagg cttccaggtc ctcgtcgacg
      181 ccggtggcgg cctcggtgac ctcgacgacc tccgccgccg gcctggcggc gacgctggcc
      241 gccaacgacg ccgcggtggc ccaggcgcag gccgccgtcg accaggcgca ggccgcggtg
      301 gacgcgaccg tgctccacgc ccctgtcgac ggcctcgtcc aggtggtgaa cgtcgtcgag
      361 gacgccgtgc cgccctcggg cgcggcgatc caggagcgct ccgacacgct caccgtgcag
      421 gcaaacgtcg ccgagcagga cgtcacgcag atcaagggcg gccagaaggc gcaggtgacc
      481 ctgccggcgc tcggcacgac cgagtccgcc acggtcgccg ggctgccttc ccaggcgaac
      541 gcggcaccgt ccagcgcgtc cgcggcggcg agcaccgcgg tcaccttccc ggtgacactc
      601 accttcgacc agtcggaccc cgcgatcctg cctggcatgt ccgcgcagct cgagatcgca
      661 ctggtccgcc acgccaacgt gctccaggtg ccgaccaccg cggtccaggg cggtgccaac
      721 ggctccaccg tgcaggtcct ccaggacggc aggccggtga cgcggccggt cgaggtcggg
      781 ctgtccaccg agaccgccac cgagatcgtc gtcggcctgc gcgagggcca gacggtcgtg
      841 accggcgtgg tcaacccgac gcagggtgcg acccagccca ccaccggtgg tggcggcttc
      901 ggcggcggag ggggagcgcg aggcggcggc ttcggcggcg gcggtggcct cggtggcggc
      961 ggaggcggcg gagcgagggg cggcggtggt ggctgatcgg ggggtcccct gggactcgcc
     1021 ggcggtgccc gggacctcgc cggcgccctg gggcgccccc gtcatcgagg cgtgggagct
     1081 ggcgcgggcc tacccgttcg ggcaggacgc cgtcgtcgcg ctgcgcggtg tgaccctgac
     1141 ggtgtggccg aacgagtcgg tggccatcat gggcgcgtcc gggtcgggca agtcgaccct
     1201 cctgcacctg ctcgggctgc tggaccggcc cacctcaggg acctaccggc tcgccggggt
     1261 cgacaccgcc acgctctccg acgaccggct cgccgagctg cgcaaccggg cgatcgggtt
     1321 cgtgttccag tcgttcaacc tggtggcggg cgagagcgcg gtggagaacg tggccgcgcc
     1381 gctggtctac caggggctgc ggcggcccga gcggctccgc cgcgccggcg aggcgctgga
     1441 gcgggtcggg ctgggcgccc ggatgcggca cgacccggcg cagctgtctg gcggggagcg
     1501 gcagcgggtc gccatcgcgc gggcactggt cacccggccg gcgctcctgc tcgccgacga
     1561 gccgaccggc aacctcgact cggcctcggg tggcgagctg ctcgcgctca tcgacgagct
     1621 gcacggcgcc gggctcacgg tggtggtggt cacccatgac ccggcggtcg cggcgcgtgc
     1681 ccagcgagtc gtgcgggtgg ccgacgggcg cgtcgtcggc gaggaggcgg gacccgtcac
     1741 ggcgggatgg agaaaccgat gagcctggcg gaggcgttcc ggctggccct gcggcgcctg
     1801 cgcgccaaca agctccgcac cgggctcacc accctcggga tcgtgatcgg cgcggcgtcg
     1861 ctggtggcgc tgctcgcggt cgggcagggc gcgcaggcga ggctgacgag ccgcatcgcg
     1921 agcctcggca cgaacctgct ctcgatccag gccggatcga gcatcgggca gggcgggatc
     1981 cgcggcgggc tcgggtcggc cagcacgctc accgtcgacg acgccgacgc gttgcggaag
     2041 ctccccggcg tggcggcggt cgcccccgag ctgcccgtcc cgggtgccct ggtggtcgcc
     2101 ggtcgagcca acaccaccac gcaggtgacg ggcaccacac ccgacgaggc gcgggtgcgc
     2161 ggctacgagg tccacgcggg cacgttcctg tccgacctcg agctccgcgc cggcctgcgg
     2221 gtcgcggtgc tgggcgccac caccgcggcc gacctcggcc tgccggcgaa gcaggcggtc
     2281 ggccagacga tccaggtcga cgcgatcccg ttccgggtga tcggcgtgac ccagcccaag
     2341 ggcggcttcg gcaaccccga cgaccaggtg ttcgtgccgc tcagcgccgt gcagcggcgg
     2401 ttgtcgggat cgtcgtcgct gcgcgcgatc ggcctgtcgg tggcgcgcgc cgacgacatg
     2461 gccgcggtgt cgcagcaggc cgagcagacc ttgcgcgccc gtcaccggct cggcgactcg
     2521 gcctccgacg acttcctgat cgccaaccag gcgcagctcc tgcaggtcgc cgcggaccag
     2581 gcgacggtgc tgcgcaactt cctgctcggc atcgccgcga tcgcgctggt cgtcggcggg
     2641 atcggcatcg ccaacatcat gctggtcgcc gtgcgcgagc ggacccgcga gatcggcatc
     2701 cgcaaggcga t
//
LOCUS       DASPYY010000753         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1671589, whole genome shotgun sequence.
ACCESSION   DASPYY010000753 DASPYY010000000
VERSION     DASPYY010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671589"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..500
                     /locus_tag="VEP73_11780"
     CDS             336..500
                     /locus_tag="VEP73_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27150.1"
                     /translation="MVTCALAECFVYASFYWKPDDGRRLRRYSCTHHLDQVDPGPGWT
                     RHGLVLPFTA"
     gene            518..1909
                     /locus_tag="VEP73_11785"
     CDS             518..1909
                     /locus_tag="VEP73_11785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT27151.1"
                     /translation="MAEEFDVVVIGAGPAGENAAGRCAQGGLRVALVEAELVGGECSY
                     WACMPSKALLRPGEALAAARRVPGASEAVTGELHAGAVLAWRDQIVNHYDDTDQVKWV
                     DGVGVELVRGRGRLGGERAVLVESPEGTRRLVAGRAVVVATGSAASVPPIEGLRDMRV
                     WDSRDVTAAKQVPRRLLVLGAGVVAVEMAQAWKRLGAEEVTIVSRDGRLLAREEPFVG
                     DELRRAFEAEGIVVRCGTTVAACRRQGADGPVTLSLEDGAELRGDELLVAAGRRPRTS
                     DLGLEAVGLEPGGVIAVDEQLRAIGVPEGWLYAVGDANGRALLTHQGKYQARLCADHI
                     LGKDVEAVADHRAVPRVIFTDPQVTAVGLTERQARDQGVDARVVTYPTGQVAGSAVAG
                     RKPVGTSQLVIDEGRRVIVGATFTGYGVAELLHAATVAIVGEVPLDRLWHAVPSFPTV
                     SEVWLRLLESYGL"
     gene            complement(1959..2780)
                     /locus_tag="VEP73_11790"
     CDS             complement(1959..2780)
                     /locus_tag="VEP73_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT27152.1"
                     /translation="MSATAPTLTGGPAGVRTGRITLGVALRHALIIARRNLLQVRGNP
                     QLLIFIAIQPILFVLLFVYVFGGAIAGSSRQYVQFVIPGIVVQTVVFSTAVTGIGLNE
                     DVSKGIVDRFRSLPIARSAVLAGRILADAVRLVATVVVILAVGMLLGFRFHRGPLAAV
                     AGFALVIAFGMALSWVAAWIGLSVRNPETAQSAGFLWMFPLTFASSVFAPPQTMPGWL
                     QPFVRINPISVAADALRALLLGGPVLAPALKTIAWTIALTVVFAPLAIRQYRQLA"
     gene            complement(2777..>3490)
                     /locus_tag="VEP73_11795"
     CDS             complement(2777..>3490)
                     /locus_tag="VEP73_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27153.1"
                     /translation="NLSGAENLSMLGRLLGQPKAQAKQRAAELLERFDLADAGGRLVK
                     TYSGGMRRRLDLAASLVGRPPVLFLDEPTTGLDPRSRNGLWAVTRELVAEGTTVLLTT
                     QYMEEADQLAGTVAVIDRGQVIAQGTPDELKAKIGGQVLEVRPADPAHLDRAAELLAD
                     LTGAPPTVNPDTRLLSVPMPDPAWLPVAVRRLDQASIAVADLALRRPSLDEVFLALTG
                     RPAEATTPQDRQEPRRSNA"
BASE COUNT          467 a         1168 c         1417 g          438 t
ORIGIN      
        1 gcagcgcagc gcggcgggcc tgggcgacgc cctcaacggc atcgcacgcc tgaccgcgcg
       61 ggccggcgcc gactacgagc aggccgagca ggggatcgcc gccagcttcg gccgcgcctg
      121 atcccggtcc ggcgccaccg ccggcgggga ccgccacggg ccgtggtggc ggtcccgccg
      181 cgcgccggcg agacggcccg gggccgaacg catctctccg ggagatggag ctggactgag
      241 caggacgacc tctcccgggc ggcaggccgc gtccgcgaca atggaggagc ggaccggacc
      301 ggccccgtag cgagtcccgc gaggaaggag acgccatggt gacctgcgcc ctggccgagt
      361 gcttcgtgta cgcctcgttc tactggaagc ccgacgacgg acgacgcctg cgacgctaca
      421 gttgcacgca ccacttggac caggtggacc ccggccccgg ctggaccagg cacggcctgg
      481 tgctgccgtt cacggcctga ccaggacgga ggctgcgatg gccgaggagt tcgacgtcgt
      541 ggtgatcggt gcgggcccgg cgggcgagaa cgccgcgggg cggtgcgccc agggaggact
      601 gcgcgtggcg ctggtcgagg ccgagctggt cggcggcgag tgctcgtatt gggcgtgcat
      661 gccctccaag gcgttgctgc gcccggggga ggcgctggcc gccgcccggc gcgtgcccgg
      721 cgccagcgag gcggtcaccg gcgagctcca cgcgggcgcc gttctggcct ggcgcgacca
      781 gatcgtcaac cactacgacg acacggatca ggtcaagtgg gtcgacggcg tcggggtcga
      841 gctggtccgc gggcgcggtc gcctgggtgg cgagcgcgct gtcctggtcg agagcccgga
      901 agggacccga cggctcgtcg ccggccgcgc cgtggtggtc gccaccggat cggcggccag
      961 cgtcccgccg atcgagggac tgcgggacat gcgggtctgg gacagccggg acgtcaccgc
     1021 ggccaagcag gtgccgcgcc ggctgctggt gctcggcgcc ggggtcgtcg ccgtcgagat
     1081 ggcgcaggcc tggaagcggc tgggcgccga ggaggtcacc atcgtctccc gggacggccg
     1141 gctgctggcc agggaggagc cgttcgtcgg cgacgagctt cggcgggcgt tcgaggccga
     1201 ggggatcgtc gtgcgctgcg gcaccaccgt tgccgcctgc cgccgccagg gagccgacgg
     1261 cccggtcacg ctgtcgctgg aggacggcgc cgagctgcgc ggcgacgagc tgctggtcgc
     1321 cgccggccgg cggcctcgca ccagtgacct cggcctggag gcggtcggcc tggagcccgg
     1381 tggggtcatc gcggtcgacg agcagctgcg ggccatcggc gtcccggaag ggtggctgta
     1441 tgcggtcggc gacgccaacg gccgggcgct cctcacccac cagggcaagt accaggcgcg
     1501 cctgtgcgcc gaccacatcc tgggcaagga cgtcgaggcg gtcgccgacc accgggccgt
     1561 gccgcgggtg atcttcaccg acccgcaggt cacggccgtc gggctgaccg agcggcaggc
     1621 ccgcgaccag ggtgtcgacg cgagggtggt cacctacccg accgggcagg tggccggctc
     1681 ggccgtggcc ggccgcaagc ctgtagggac cagccagctc gtcatcgacg agggccgccg
     1741 ggtgatcgtc ggggccacct tcaccgggta cggcgtggcc gagctgctgc acgcggcgac
     1801 ggtcgcgatc gtcggtgagg tcccgctgga ccggctgtgg cacgcggtgc cgtccttccc
     1861 gacggtgagc gaggtctggc tccgcctgct cgagtcgtac ggcctgtgac gcccgcgtca
     1921 ggggcgtaga tggcggcgtc gatgcggccg cggtcgggtc aggcgagctg gcggtattgg
     1981 cggatggcga ggggtgcgaa caccacggtg agggcgatgg tccaggcgat cgtcttgagg
     2041 gccggtgcga ggaccgggcc gcccagcagc agggcgcgca gcgcgtcggc ggcgacgctg
     2101 atcgggttga tgcggacgaa cggttgcagc cagcccggca tggtctgggg tggggcgaac
     2161 accgagctgg cgaaggtcag cgggaacatc cagaggaacc cggccgactg ggccgtctcc
     2221 gggttgcgga ccgacaggcc gatccaggcg gccacccagg acagggccat gccgaaggcg
     2281 atgaccagcg cgaacccggc cacggcggcc agcgggccgc ggtggaagcg gaagccgagc
     2341 agcatcccga cggcgaggat gaccaccacg gtggcgacca ggcgcacggc gtcggccagg
     2401 atgcggccgg ccaggaccgc ggagcgggcg atcggcaggg accggaaccg gtcgacgatg
     2461 cccttggaga cgtcctcgtt gagcccgatg ccggtgacgg cggtggagaa cacgaccgtc
     2521 tggacgacga tgcccgggat cacgaactgg acgtactggc ggctggaccc ggcgatggcg
     2581 ccgccgaaca cgtagacgaa caacagcacg aacaggatcg gctggatggc gatgaagatc
     2641 agcagctggg ggttgccgcg gacctggagg aggttgcggc gggcgatgat cagcgcgtgg
     2701 cggagcgcga cgcccagggt gatccgtccc gtgcgcacgc ccgcggggcc gccggtcagg
     2761 gtcggtgcgg tggcgctcat gcgttgctcc ttcgcggctc ctgcctgtct tggggtgtcg
     2821 tcgcttcggc ggggcggccg gtcagggcca ggaacacctc gtccaggctc gggcggcgca
     2881 gcgcgaggtc ggcgacggcg atgctggcct ggtccaggcg ccggacggcg accgggagcc
     2941 aggccgggtc gggcatcggc acgctgagca ggcgggtgtc cgggttgacg gtgggcgggg
     3001 cgccggtcag gtcggccagc agctcggcgg cccggtcgag gtgggccggg tcggccgggc
     3061 ggacctcgag gacctggccg ccgatcttgg ccttgagctc gtcgggggtg ccctgggcga
     3121 tgacctggcc gcggtcgatc accgcgaccg tgccggcgag ctggtcggcc tcctccatgt
     3181 actgggtggt gagcagcacg gtggtgccct cggcgaccag ctcccgggtc accgcccaca
     3241 ggccgttgcg gctgcgtggg tccagcccgg tggtcggctc gtccaaaaac agcacgggtg
     3301 gccggccgac caggctggcg gccaggtcca ggcgccggcg catcccgccc gagtaggtct
     3361 tcaccagccg gccgccggcg tcggccaggt cgaagcgctc aagcagctcg gcggcgcgct
     3421 gcttcgcctg ggccttgggc tggccgagca gccggcccag catcgagagg ttctcggcgc
     3481 cgctcaggtt
//
LOCUS       DASPYY010000754         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1391235, whole genome shotgun sequence.
ACCESSION   DASPYY010000754 DASPYY010000000
VERSION     DASPYY010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391235"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VEP73_11800"
     CDS             <1..435
                     /locus_tag="VEP73_11800"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HYT27154.1"
                     /translation="VADYEPITGRVHQPPPVHQVFEGSSFVVCNFVPRKVDYHPLSIP
                     VPYYHSNVDSDEVMFYVAGDYEARKGSGIGVGSISLHPGGYAHGPQPEAIERAIGAEF
                     FDELAVMVDTFRPLELGEAGRACEDPAYAWTWSGRGPARDAG"
     gene            449..1624
                     /gene="pobA"
                     /locus_tag="VEP73_11805"
     CDS             449..1624
                     /gene="pobA"
                     /locus_tag="VEP73_11805"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HYT27155.1"
                     /translation="MRTQVGIVGAGPAGLLLSHLLARRGVESVVLEARSRDYVEHRVR
                     AGVLEQNTVDLLRETGVGERMDREGLVHHGIELRFEGRGHRIPMTELTGGRSITVYGQ
                     QEVVKDLIRARLEAGGQLVFQAGAVGLHGIGSDRPAVRYREDGEDRELECDVVAGCDG
                     FHGVCRDAVPAGVLRVDERAYPYAWLGVLAAVPPSTEELIYCSHERGFALHSMRSPKL
                     TRLYLQVDPEEAIDAWPDERIWKELQTRLATDDGWTLADGPILEKGITPMRSFVAEPM
                     RFGRLFLAGDAAHIVPPTGAKGMNLAVADVRVLAEALAAWFRTGATDLMDGYSDTCLR
                     RVWRAQDFSSWMTRMLHRSPGDDGFERRIQRARLAYVVSSEAAARSLAENYVGLERL"
     gene            1797..>2299
                     /locus_tag="VEP73_11810"
     CDS             1797..>2299
                     /locus_tag="VEP73_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT27156.1"
                     /translation="MSETVTAGGRRQPEARLLVVEDEPNILELLSASLRFAGFEVATA
                     TGGLEALHAAQRERPDLVVLDVMLPDLDGFDVARRLRSGNARIPVLFLTARDATEDKV
                     AGLTLGGDDYVTKPFSLEEVVARIRAVLRRARGDTLPTPTRLRVADLELDEDSHEVWR
                     AGNRVQLS"
BASE COUNT          319 a          803 c          873 g          304 t
ORIGIN      
        1 gtggccgact acgagccgat caccgggcgg gtccaccagc cgccacccgt ccaccaggtg
       61 ttcgaggggt cgagcttcgt ggtctgcaac ttcgtgccgc gcaaggtgga ctaccacccg
      121 ctgtcgatcc cggtgcccta ctaccactcc aacgtcgact ccgacgaggt gatgttctac
      181 gtcgccggcg actacgaggc gcgcaagggg tccgggatcg gcgtcggctc gatctccctg
      241 caccctggcg ggtacgcgca cgggccgcag cccgaggcga tcgagcgggc gatcggcgcg
      301 gagttcttcg acgagctcgc ggtgatggtg gacacgttcc gcccgctcga gctcggcgag
      361 gcggggcggg cgtgcgagga cccggcctac gcctggacgt ggagcgggcg cggccccgcc
      421 agggacgccg ggtgaggaag gacggcccat gcgcacccag gtggggatcg tcggggccgg
      481 gccggccggg ctgctgctgt cgcacctgct cgcccggcgg ggcgtggagt cggtcgtgct
      541 cgaggcccgc agccgcgact acgtggagca tcgggtgcgg gccggggtgc tcgagcagaa
      601 cacggtcgac ctgctgcgcg agaccggggt cggggagcgg atggaccgcg aggggctggt
      661 ccaccacggc atcgagctgc gcttcgaggg acgcgggcac cgcatcccca tgaccgagct
      721 gacgggaggg cgctcgatca ccgtctacgg gcagcaggag gtcgtcaagg acctcatccg
      781 ggcccggctc gaggcgggcg gccagctcgt gttccaggcc ggcgcggtcg gcctccacgg
      841 gatcgggtcc gaccggccgg ccgtccgcta ccgcgaggac ggcgaggacc gcgagctcga
      901 gtgcgacgtc gtcgccggct gcgacggctt ccacggcgtg tgccgggacg cggtccccgc
      961 cggggtcctc cgcgtggacg agcgcgccta cccctacgcc tggctcggcg tgctcgccgc
     1021 cgtgcccccg tcgaccgagg agctgatcta ctgctcccac gagcgtggct tcgcgctgca
     1081 cagcatgcgg tcgcccaagc tgacccggct gtacctgcag gtcgaccccg aggaggcgat
     1141 cgatgcctgg ccggacgagc ggatctggaa ggagctgcag acgcggctcg cgaccgacga
     1201 cggctggacg ctcgcggacg ggccgatcct cgagaagggg atcacgccca tgcgcagctt
     1261 cgtcgccgag ccgatgcggt tcggccggct gttcctggcc ggcgacgccg cgcacatcgt
     1321 cccgccgacc ggcgccaagg ggatgaacct ggccgtggcg gacgtgcggg tgctggccga
     1381 ggcgctcgcc gcctggttcc ggaccggcgc caccgacctg atggacgggt actcggacac
     1441 ctgcctgcgc cgggtctggc gggcccagga cttctccagc tggatgacga ggatgctcca
     1501 ccggtcgccg ggcgacgacg ggttcgagcg ccggatccag cgcgcccggc tcgcctacgt
     1561 ggtgtcgtcg gaggcggccg cgcggagcct cgccgagaac tacgtcgggc tcgagcgcct
     1621 ctgacacacg cgtctgacac ggccgagcca cgcgacgggc cgtagcatgt tgaggcggcc
     1681 ggccaggggt tcagcggatt cccaggaaag gctcaggctc gcgcaagctg cacgaccgac
     1741 catgaccacg tcaggtcgcg tcgcacggga gccagagcat gtcgacagga agcacgatga
     1801 gcgagacggt cacagcgggt gggcggcggc agccggaggc gcggctgttg gtcgtcgagg
     1861 acgagcccaa catcctcgag ctgctgtcgg cgagcctccg gttcgccggg ttcgaggtcg
     1921 ccacggcaac gggtggcctg gaggcgctgc acgccgccca gcgggagcgc cccgacctgg
     1981 tggtgctgga cgtgatgctg ccggacctgg acggcttcga cgtcgcgcgc cggctgcgct
     2041 ccggcaatgc ccggatcccg gtgctgttcc tgaccgcccg ggacgccacc gaggacaagg
     2101 tcgccgggct gacgctcggc ggcgacgact acgtcaccaa gccgttcagc ctcgaggagg
     2161 tcgtcgcccg gatccgcgcc gtgctgcgcc gggcgcgggg cgacaccctc ccgacgccga
     2221 cccggctgcg ggtcgccgac ctcgagctgg acgaggacag ccacgaggtc tggcgcgccg
     2281 gcaaccgggt ccagctctc
//
LOCUS       DASPYY010000755         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_124450, whole genome shotgun sequence.
ACCESSION   DASPYY010000755 DASPYY010000000
VERSION     DASPYY010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124450"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2504
                     /locus_tag="VEP73_11815"
     CDS             <1..2504
                     /locus_tag="VEP73_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT27157.1"
                     /translation="RFLAERSVVIGRDRRLGVERYSTPELLERERELVAAATQRREQG
                     CAQVRPDVVRSVLDRHATAGEDQAAMVEDVTRSGAGVSVVVGRAGSGKTWALGLAREA
                     FELDGHRVLGAAPTGIAAVGLGDEGFSDIRTVDRLLFDLGSGRAELDAKTVLVVDEAA
                     MLGTRKLARLLDHAHPARAKVVLVGDDRQFASIEAGGGFRALRLRLGASELTVNRRQV
                     AAWEQRAIDDLREGRVEQAIAAYAEHDRIRAFDARDDRDRALVGDWWTAHQAGEQPVI
                     YAHRRAQVDQLNTVCQRLRAEHGQLGVERLAVGDRAFAVGDRVVLGANALDRLGVASG
                     TSAEITALDVRGRTMTIRVLEEDPPRTVTLPGWYLDAAVRPGQSRRVDLAYARTDMRS
                     QGRTEQRALLALDGAEDMQGSYVQLTRGKERTDMYLTVGPEPLGDTEAHPHPRGEPVE
                     PARLLGRVLTRDGSKALASDTPTLLDVRRLSTGQLRAERDRLAELRATCPADRSRELA
                     LARSRAAELDAARLTAVAEQQAAEAALAEVGGGLLRRREAAATRERLTLAEHAVRATG
                     RQATQAAERAGRLRRAEAERAGWLEQHADLPAAERATARELAWRHRVDARAVALDQPG
                     WLTAELGPMPPAEQPAGRAAWLAAAVELDTWRRTHGLDSERPAEQGRGQRTRAWRDGA
                     RGERATARLLRPLTRHGHVVFHDVAIPGTPANADHLLISTGGVVLVDAKRYTGRVWQA
                     PDGRVWHNQYLMDRPLRALRLETAAIERALGVPVRPVVCVHGAHVDFGGLVAGEVEIV
                     PAGRLRGLLTAAPPLRSQADLASLVAYARAMLRPA"
     gene            2588..>2812
                     /locus_tag="VEP73_11820"
     CDS             2588..>2812
                     /locus_tag="VEP73_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27158.1"
                     /translation="MSELGPNAFGEPVDPGRVDCYLGLPLALDDDEGRGGVLVVAPRW
                     LLDPHVPQPAGSLVYELVTGHPDRHPGELVF"
BASE COUNT          338 a         1090 c         1072 g          312 t
ORIGIN      
        1 agcggttcct ggctgagcgc tcggtcgtga tcggccgcga ccggcggctg ggggttgagc
       61 gctactccac ccccgagctg ctcgagcggg agcgggagct ggtcgccgcc gccacccagc
      121 gccgcgagca gggctgcgcc caggtccgcc ccgacgtcgt ccgctccgtc ctcgaccgcc
      181 acgccaccgc cggggaggac caggccgcga tggtggaaga cgtgacccgc tcgggcgcgg
      241 gcgtctccgt ggtggtcggg cgggccggca gcggcaagac ctgggcgttg gggctggcaa
      301 gggaggcgtt cgagctggac ggccaccggg tgcttggcgc cgcgccgacc gggattgccg
      361 cggtggggct gggcgacgag ggcttcagtg acatccgcac ggtcgaccgg ctgctgttcg
      421 acctcggctc cggccgcgcc gagctggacg ccaagaccgt cctggtggtc gacgaggccg
      481 cgatgctcgg cacccgcaag ctcgcccggc tgctcgacca cgcccacccc gcccgggcga
      541 aggtcgtcct ggtcggcgac gaccggcagt tcgcctccat cgaagcgggc ggcggcttcc
      601 gcgccctcag gctgcggctg ggcgcctcgg agctgaccgt caaccgccgg caggtggccg
      661 cgtgggagca gcgcgccatc gacgacctgc gcgaagggcg agtggagcag gcgatcgccg
      721 cctacgccga gcacgaccgc atccgcgcgt tcgacgccag agacgaccgc gaccgcgcgc
      781 tcgtagggga ctggtggacc gcgcaccagg cgggcgagca gccggtcatc tacgcccacc
      841 gccgcgccca ggtcgaccag ctcaacaccg tctgccagcg gctccgcgcc gagcacggcc
      901 agctcggggt ggagcgactc gcggtcgggg accgggcctt cgcggtcggc gaccgggtgg
      961 tgctgggcgc gaacgcgctg gaccggctgg gggtcgccag cggcacctcc gccgagatca
     1021 ccgcgctaga cgtgcgcggg cggaccatga cgatccgcgt gctcgaggag gacccgccgc
     1081 gcacggtcac cctgcccggc tggtacctgg acgccgcggt gcggccgggc cagtcccggc
     1141 gggtggatct ggcctacgcg cggaccgaca tgcgctccca gggccgcacc gagcagcgcg
     1201 ccctgctcgc gctggacggc gccgaggaca tgcaggggtc ctacgtgcag ctcacccgcg
     1261 gcaaggaacg caccgacatg tacctgaccg tcggcccaga accgctcggc gacaccgaag
     1321 cgcacccgca tccccggggc gagccggtcg agccggcgcg gctgctcggc cgggtgctga
     1381 cccgcgacgg ctccaaggcc ctcgccagcg acaccccgac cctgctggac gtgcggcggc
     1441 tgtcgaccgg gcagctgcgg gcggaacgcg accggctcgc ggagctgcgc gccacctgcc
     1501 cggcggaccg ctcgcgggag ctggcgctgg cccgctcccg cgccgccgag ctggacgccg
     1561 cccgcctgac cgcggtcgcc gagcagcaag ccgccgaagc ggccctcgct gaggtgggcg
     1621 gcggcctact gcgccgccgg gaagccgccg cgacgcggga gcggctgacc ttggcggagc
     1681 atgcggtgcg ggccaccggc cggcaggcca cccaggccgc cgagcgggcc gggcggctgc
     1741 gccgcgccga ggccgagcgc gccggctggc tggaacagca cgccgacctg cccgcggccg
     1801 agcgcgccac cgcccgagag ctggcctggc ggcaccgggt ggacgcccgc gcggtcgcgc
     1861 tcgaccagcc cggctggctg accgccgagc tcggcccgat gcccccggcc gagcagccgg
     1921 ccgggcgggc ggcgtggctg gccgccgcgg tcgaactgga cacctggcgg cgcacccacg
     1981 ggctcgacag cgagcggccc gctgagcagg ggcgggggca gcgcacccgg gcgtggcgcg
     2041 acggcgcccg cggggagcgc gccaccgccc gcctgctccg cccgctcacc cgccacgggc
     2101 acgtggtctt ccacgatgtg gcgatccccg gcacccccgc caacgccgac cacctgctga
     2161 tctcaaccgg cggggtggtg ctggtggacg ccaaacgcta caccggccgg gtctggcagg
     2221 ccccggacgg gcgcgtgtgg cacaaccagt acctcatgga ccgacccttg cgcgcgctgc
     2281 gcctggagac cgccgcgatc gagcgcgccc tgggggtgcc ggtgcgtccg gtggtctgcg
     2341 tccacggcgc ccacgtcgac ttcggcggcc tggtcgccgg ggaggtggag atcgtgcccg
     2401 ccgggcggct ccgcggcctg ctcaccgccg ccccgcccct gcgcagccag gcggaccttg
     2461 cctccctggt cgcctacgcc cgcgccatgc tccgccccgc ctgacggccc acgcgcggcc
     2521 acagggacgg cggcgggcgg ggtcggtagc ctggtgccct accccgacca tgggaggcac
     2581 gcggctggtg agcgagcttg gccccaacgc cttcggggag ccggtcgacc ccggccgggt
     2641 cgactgctat ctcggcctgc ccctcgccct cgacgacgac gaggggcggg gtggggtgct
     2701 ggtggtggcg ccccgctggc tgcttgaccc tcatgttccc cagcctgccg gcagcctggt
     2761 ctacgagctg gtcaccggcc accccgaccg gcaccccggc gagctggtct tc
//
LOCUS       DASPYY010000756         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1391516, whole genome shotgun sequence.
ACCESSION   DASPYY010000756 DASPYY010000000
VERSION     DASPYY010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391516"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /gene="manB"
                     /locus_tag="VEP73_11825"
     CDS             complement(<1..1184)
                     /gene="manB"
                     /locus_tag="VEP73_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472763.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYT27159.1"
                     /translation="MPADLDPADLEKVFKAYDVRGVYPDELNEDLARATGRAFVALSG
                     ATSVAIGHDMRPSSGPLTDALTDGATSQGADVVRVGLCSTDMLYFVSGDLDVAGVMLT
                     ASHNPARYNGMKLCRPGAVPVGEDSGLLDIKRMVASGEPRGDAERRGSVADGEDLLDR
                     YARHVRGFVDVSALRPLKVVADAGNGMAGHVVPTVFEGLPFQLDPMYFELDGNFPNHP
                     ADPINPANLADLRQRVVATGADVGLAFDGDADRVFLVDEQGGIVPSSLVVALVAKTMC
                     EREPGATVLYNLICSHVVPETVRAAGGNPVRTRVGHSFIKKVMADTGAIFGGEHSGHY
                     YFRDNYRADSGLIAALVTLEVLCRADRPLSEVLAPFRKYADSGEINRRVDGDPKEVVA
                     RVA"
     gene            complement(1288..1464)
                     /locus_tag="VEP73_11830"
     CDS             complement(1288..1464)
                     /locus_tag="VEP73_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27160.1"
                     /translation="MQCPTCSSSSLTAIRIRLAPEEEHTFASCHACEWKGWSREGSAV
                     PLERVLSLASERRF"
BASE COUNT          229 a          608 c          667 g          229 t
ORIGIN      
        1 gccacccggg cgacgacctc cttcgggtcg ccgtcgaccc ggcggttgat ctcccctgag
       61 tcggcgtact tccggaaggg ggcgaggacc tccgagagcg gccggtcggc gcggcagagg
      121 acctcgaggg tgacgagggc cgcgatgagc ccggagtcgg cccggtagtt gtcgcggaag
      181 tagtagtggc cggagtgctc gccgccgaag atggcgccgg tgtcggccat gaccttcttg
      241 atgaacgaat ggccgaccct ggtccgcacc gggttgcccc cggcggcacg gaccgtctcg
      301 ggcacgacgt gggagcagat gaggttgtag agcacggtcg cgcccggctc gcgctcgcac
      361 atcgtcttgg cgacgagcgc gaccacgagg ctggagggca cgatgccgcc ctgctcgtcg
      421 acgaggaaga cccggtcggc gtccccgtcg aaggcgagcc cgacgtccgc gcccgtggcg
      481 accacccgct ggcggaggtc ggccaggttc gccgggttga tcgggtcggc cgggtggttg
      541 gggaagttgc cgtccagctc gaagtacatg gggtcgagct ggaacggcag gccctcgaag
      601 acggtgggca cgacgtggcc tgccatgccg ttgcccgcgt cggcgacgac cttgagcggg
      661 cgcagggcgc tcacgtccac gaagccgcgg acgtgcctgg cgtagcggtc gagcaggtcc
      721 tcgccgtcgg cgacgctccc ccgccgctcg gcgtcgccgc gcggctcccc ggacgccacc
      781 atccgcttga tgtcgagcag gccggagtcc tccccgaccg gcacggcgcc cgggcggcag
      841 agcttcatgc cgttgtagcg cgccgggttg tgcgacgcgg tcagcatcac gcccgcgacg
      901 tcgaggtcgc cggacacgaa gtacagcatg tcggtcgagc acagcccgac ccggacgacg
      961 tcggcgccct ggctggtcgc gccgtcggtc agggcgtcgg tcagcgggcc ggacgagggc
     1021 cgcatgtcgt ggccgatcgc gaccgacgtc gcgccgctga gcgccacgaa cgcacggccg
     1081 gtcgcgcggg ccaggtcctc gttgagctcg tcggggtaga cgccgcggac gtcgtaggcc
     1141 ttgaacacct tctcgaggtc ggcggggtcg aggtcggcgg gcacgcgaag ctcctcggga
     1201 cggcctccgg gcacgggagg cgacggctgc gaagccgcat tctacccacc cgccgcacgc
     1261 cagccgcccc ccgggggtcc gcgcgggtca gaaccggcgc tcggaggcca gggagaggac
     1321 gcgctcgagg ggcaccgcgc tgccctccct ggaccagccc ttccactcgc aggcgtgaca
     1381 ggaggcgaag gtgtgctcct cctcgggagc gagccggatc ctgatcgcgg tcaggctgct
     1441 ggagctacag gtgggacact gcatacggga cctcgacggt tggccggttt cggcggctgc
     1501 catgacgcgg cccgcgccgg gcacggcgtc cgctcgggag cgggccggtg gtggcgcgga
     1561 cctgcttcca tgggtcggca ggggcccggc accgccgcat ggcccgatgg gacgccccgc
     1621 accgccctca ggggctagac acggcgggcc tcgcgcgctt caggcgtcgg gggcggcgcc
     1681 cgggcgacgt tcgggtctcc cctaccggtc cccgcgggca ccgccgccgc gca
//
LOCUS       DASPYY010000757         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1253042, whole genome shotgun sequence.
ACCESSION   DASPYY010000757 DASPYY010000000
VERSION     DASPYY010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253042"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..709)
                     /locus_tag="VEP73_11835"
     CDS             complement(119..709)
                     /locus_tag="VEP73_11835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT27161.1"
                     /translation="MSADLVYEDGIRLPLELERWLGDPDPVDRDLVRWVLAPVLDVGC
                     GPGRHVVALAAVGIPALGLDVAPSAVHLARTRGACVLERSVFGRVPGAGRWATALLLD
                     GNAGIGGDPEALLARLRTLLRPGGRILVEVEEPGVAGARLHARIETPVRSSSWFPWAR
                     VGVDDLERLGVACRLAVREVWQRGGRWFGLMETSRP"
     gene            complement(706..1416)
                     /locus_tag="VEP73_11840"
     CDS             complement(706..1416)
                     /locus_tag="VEP73_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYT27162.1"
                     /translation="MRPRLIVLAKAPVPGLSKTRLCPPCTPVQAAAVAEAALADTLSA
                     VMRVGGIRPLLVLDGTAGAWMPRGLGVVPQRGGGLDERLAAAFEDCGGPALLIGMDTP
                     QVTPEVLGGCVDALLAPGVDAVLGPAIDGGWWAAGLRRPRPSAFLGVPMSTGRTCDAQ
                     RRRLAALGLEVGELPALQDVDKWENALSVFGGIAASGAPGPSLRGGCYSPRTPLGDSA
                     ESRFAAAVDAVARALVAG"
     gene            complement(1413..>1502)
                     /locus_tag="VEP73_11845"
     CDS             complement(1413..>1502)
                     /locus_tag="VEP73_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT27163.1"
                     /translation="YLPRRGRSKVTGTVAGTLRAARDMRRLLA"
BASE COUNT          234 a          577 c          538 g          153 t
ORIGIN      
        1 tggatcaggt gtgagagcag gcccgtaaga gagcagatcc cgaaggcgac cccgaggcag
       61 atgccgagcc acgccgcgtt gcgctcggag tgcaggcctc cctccgtcac gccggtgctc
      121 atggccggga ggtctccatc agcccgaacc accggccgcc gcgctgccag acctcgcgca
      181 cggcgagccg gcacgcgacc cccagccgct ccaggtcgtc gacgccgacg cgcgcccacg
      241 ggaaccacga cgatgagcgc accggcgtct cgatccttgc atgcaggcgg gcgccggcaa
      301 cccccggctc ctccacttcc acgaggatcc tgccgcctgg acgcaggagg gtcctcaggc
      361 gcgcgagcaa agcctccggg tcgccgccga tgccggcgtt gccgtcgagc aggagggccg
      421 tggcccagcg cccggcaccg ggcacccgac cgaagaccga gcgttccaac acgcaggcgc
      481 cccgggtacg cgccaggtgg acggcgctcg gcgccacatc gagcccgagc gccgggatgc
      541 ccaccgccgc cagggctacg acgtggcgcc cgggcccgca gccgacgtcg agcaccggcg
      601 cgaggaccca ccggaccagg tcgcggtcga cggggtcggg gtcgccgagc cagcgctcga
      661 gctccagcgg cagccggata ccgtcctcgt agacgaggtc cgcgctcacc cggccaccaa
      721 tgcccgcgca accgcgtcga ccgccgccgc gaagcgcgat tcagcagagt cgcccagggg
      781 ggttcggggg gagtagcagc cccctcgcag cgaggggcct ggcgcgccag aggctgcgat
      841 ccccccgaag acagataagg cgttctccca cttgtcaacg tcttggaggg cggggagttc
      901 cccgacctcc agccccagcg cggccaggcg acgccgctgc gcgtcgcacg tgcggccggt
      961 gctcatgggc accccgagga acgctgaggg ccggggccgc cgaagccccg cagcccacca
     1021 tccaccgtcg attgccgggc cgaggaccgc gtcgacgccg ggggccagga gggcatcgac
     1081 gcagccgccc agcacctcgg gcgtgacctg cggggtgtcc ataccgatca ggagggccgg
     1141 gcccccgcag tcctcgaacg ccgcggcgag acgctcgtca aggccgccac cgcgctgggg
     1201 gaccacgccg agcccccgtg gcatccaggc cccggccgtt ccatccagga cgagcagcgg
     1261 ccggatcccg ccaaccctca tgaccgctga cagggtgtcg gcaagggcgg cctcggcgac
     1321 ggcagcggcc tggacggggg tgcagggagg gcacagccgc gtcttcgaca gcccgggcac
     1381 cggggccttg gcgaggacga tcagccgggg cctcatgcca gcaaccgccg catgtccctg
     1441 gcggcgcgca atgtcccggc gacggtgccg gtcaccttcg agcggcccct gcggggcagg
     1501 ta
//
LOCUS       DASPYY010000758         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_267943, whole genome shotgun sequence.
ACCESSION   DASPYY010000758 DASPYY010000000
VERSION     DASPYY010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267943"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VEP73_11850"
     CDS             complement(<1..314)
                     /locus_tag="VEP73_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYT27164.1"
                     /translation="MRALRFHKARELHVEQVAEPAPPGPGEVLLRPLMCGICGTDLHE
                     YTDGPIVTPARPHPLTGAELPQILGHEFAATVASAGPGVTHVRPGDLCTVMPLIHCGR
                     CP"
     gene            complement(318..827)
                     /locus_tag="VEP73_11855"
     CDS             complement(318..827)
                     /locus_tag="VEP73_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT27165.1"
                     /translation="MSIAMRAQSHVGITVRDLDASVRFYHDLLGLEICTEPSPWFDEP
                     GLGQGVGVPGAALRQVCLRLGDTMLELLEYRTPPSDTERPLLSHSIGASHVAFGVDDI
                     QAAKAELESQGVTFYSDVNVVDDGVLAGWRWVYFADPDGYPLELVENAYERPGGHAAG
                     IARYLKGRR"
     gene            complement(824..1855)
                     /locus_tag="VEP73_11860"
     CDS             complement(824..1855)
                     /locus_tag="VEP73_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYT27166.1"
                     /translation="MRAAVFRAPGDVRVESVPEPPPPGPGDLLIKVSKAALCGTDSAE
                     WDHGPLLARPPVILGHEFTGEVVAAGPETNGFPPGTRVVSGAGISCGRCDWCAAGRTN
                     LCARYQTLGLHRDGGLAELVLSPAGICRAVPDGLDDTGAAMAQPLAVALHAARRGRVG
                     PGRSCAVIGAGGIGAFIVAAASALGASPLIAVDVATSRLDTAAALGATHTARAEDAVP
                     AVLAATSGDGAHVVIEASGAPDGPGAALSMVRRGGDVVFVGLQARPFGIDLFDVSTRE
                     IDLHGTLAHVCGEDLAEAVSLLARTPLAGTVLGGVIPLDSLVEGGLRPLAERKATGKI
                     VVDVAGGTW"
     gene            complement(1852..>2466)
                     /locus_tag="VEP73_11865"
     CDS             complement(1852..>2466)
                     /locus_tag="VEP73_11865"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYT27167.1"
                     /translation="EWLHKLPEHLTFTQGALVEPFSVAYAAVRAGVVDPSDRVAVLGG
                     GPIGLLSAMAAVGRNAAVTLIEPRPDRRAKAAELGVSTTLDPAEVGGELFDVVIESAG
                     HPEAMAQALTIAGNHGRVVYVGIDVGRTVPAELGLIQARSLRIQGIVGSAGVWPQAIR
                     FLASGVVDPTPIVTARYPLADAPAALKAASLGSGNIKVHIEVAP"
BASE COUNT          328 a          886 c          937 g          315 t
ORIGIN      
        1 tcggggcagc ggccgcagtg gatcagtggc atgaccgtgc acaggtcgcc cggcctgacg
       61 tgggtgacgc ccgggccggc cgaggcgacc gtggcggcga actcgtggcc gaggatctgc
      121 ggcagctcgg cgccggtcag cgggtgcggg cgggccgggg tgacgatcgg cccgtcggtg
      181 tactcgtgca ggtcggtgcc gcagattccg cacatcagcg gccgcagcag gacctcgcca
      241 ggacccggcg gtgccggctc ggcgacctgt tcgacgtgca gctcgcgtgc cttatggaag
      301 cgcagggcgc gcatggctca ccttctcccc ttgaggtacc gggcgatgcc cgcggcgtgg
      361 ccgcccggcc gttcgtaggc gttctcgacg agttccagcg ggtagccgtc ggggtcggcg
      421 aagtacaccc agcgccagcc ggccagcacg ccgtcgtcca ccacgttgac gtcggagtag
      481 aaggtgacgc cctggctctc cagttccgcc ttggccgcct ggatgtcgtc gactccgaag
      541 gcgacgtggc tggcgccgat ggagtgcgag agcagcggcc gttcggtgtc cgacggcggc
      601 gtccggtact ccagcagctc cagcatggtg tcgccgagcc gcaggcagac ctggcgcagc
      661 gcggcgccgg gcacgccgac gccctggccg aggccgggct cgtcgaacca ggggctcggc
      721 tcggtgcaga tctccaggcc gagcaggtca tggtagaagc gaacggatgc gtcgagatcc
      781 cgcacggtga tcccgacgtg gctctgcgcc cgcatggcga tgctcaccag gttcctcctg
      841 cgacgtcgac gacgatcttg ccggtggcct tgcgctcggc cagcgggcgc aggccgccct
      901 cgaccaggct gtcgagcggg atcacgccgc cgaggacggt gccggccagc ggcgtgcggg
      961 ccagcagcga gacggcttcg gccaggtcct cgccgcatac gtgggcgagc gtcccgtgca
     1021 ggtcgatctc ccgggtggac acatcgaaaa gatcgatacc gaacgggcgg gcctggagcc
     1081 ccacgaacac cacatcgccg ccccggcgga ccatgctcag cgcggcgccg gggccgtccg
     1141 gcgccccgga cgcctcgatg accacatgcg caccgtctcc gctcgtcgcg gccagcacgg
     1201 ctggtaccgc gtcttcggcc cgggccgtgt gggtggcgcc gagcgcggcg gcggtgtcga
     1261 gacggctcgt ggcgacgtcg acggcgatga gcgggctggc gccgagtgcg gacgcggcgg
     1321 ccacgatgaa cgccccgatg ccgcccgcgc cgatgacggc gcaggaacgg cccggcccga
     1381 cccggccgcg ccgggccgcg tgcagcgcga cggccagcgg ctgggccatg gccgcgccgg
     1441 tgtcgtcgag gccgtcgggc actgcccggc agattcccgc cggggacagg acgagttcgg
     1501 ccaggccgcc gtcccggtgc aggccgagcg tctggtagcg ggcgcacagg ttggtccggc
     1561 ccgcggcgca ccagtcgcac cggccgcagg agatcccggc cccgctgacc acccgggtgc
     1621 cgggcgggaa cccgttcgtc tccggcccgg cggcgaccac ctcgccggtg aactcgtggc
     1681 cgaggatcac cggggggcgg gccagcaacg ggccgtggtc ccattcggcc gaatcggtgc
     1741 cgcacagcgc cgccttgctg accttgatga gcaggtcgcc cgggccgggc ggcggcggct
     1801 cgggcaccga ctcgacccgg acgtcgccgg gggcgcggaa gacggccgcc ctcacggggc
     1861 gacctcgatg tgtaccttga tgttgcccga gcccagggac gcggccttga gcgctgcagg
     1921 cgcgtcggcc agcgggtagc gggccgtcac gatcggggtc gggtcgacga cgccgctggc
     1981 caggaacctg atcgcctgcg gccagacgcc ggccgatccg acgatgccct ggatgcgcag
     2041 cgaccgggcc tggatcaggc ccagctcggc cgggaccgtg cggccgacgt cgatacctac
     2101 gtagacgacc cggccgtggt tcccggcgat ggtgagcgcc tgcgccatcg cctccgggtg
     2161 cccggccgac tcgatcacca cgtcgaacaa ctccccgccg acttctgccg ggtcgagggt
     2221 ggtggaaaca ccgagctcgg cggccttggc ccggcggtcg ggccggggct cgatcagcgt
     2281 cacggccgcg ttgcggccga ccgcggccat ggccgacagc agcccgatcg ggccgccgcc
     2341 gagcaccgcg acccggtcgc tcgggtcgac gacgccggcc cggaccgccg cgtaggcgac
     2401 gctgaacggc tcgaccagcg caccctgggt gaaggtcagg tgttccggga gcttgtgcag
     2461 ccactc
//
LOCUS       DASPYY010000759         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2797084, whole genome shotgun sequence.
ACCESSION   DASPYY010000759 DASPYY010000000
VERSION     DASPYY010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797084"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VEP73_11870"
     CDS             complement(<1..1466)
                     /locus_tag="VEP73_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27168.1"
                     /translation="MARQIAENEGEQRATATAVGRARQRHRDLEDSPEARSHQQVLAL
                     GERAAELVAHAGKARRHAQDARGRAGKAGQRAEHDAASLRRDVTGLAASGQSAAEFAA
                     LGLPALVIVPAVLLDPAMLDDMPVLDQALAGHARAAGAARPRVGERQAAVRVVEEARR
                     GAERAESQRRSAEERAAELEGAAQDERVRLAAAERQARSAQESFEADLAAWRADDRAV
                     PVEIPDELTEQAVQRLGGIAREAADPLLRELAVAGDRAASERAGLEAALESSGLLDAW
                     VRVDGQVLGADQRDVVLPLGPAVPGRSLADLLIVDCPGDCPVSAATIRGVLARIAPCG
                     TGGTGATGASAVGGASSVGGTSEPNGTDETDETGAVVGTDGRWRLGPLTGRAAKPAAQ
                     YIGATARAAERARRLAEVDRIAEATRHARDAAAAAEEAARGRRSAIEAWLGAVPPFGE
                     LLAATADARACAVASARAEQAARAAEEAARQARAAAAAA"
     gene            complement(2377..>3457)
                     /locus_tag="VEP73_11875"
     CDS             complement(2377..>3457)
                     /locus_tag="VEP73_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HYT27169.1"
                     /translation="AEEFRLVRRHRGDLVRMFADGLGYRLVVDPHAARLFKPGLGRDP
                     SRPLEKAGGAAFTPRAYALLCLTLAALTQARGQLLVDELVAAIRSAAAEAGLDVDLDQ
                     IGDRRALYAAITALIRYGVLRERDGDLERWASDGRTATLLDVRRDRLSLMVSAPLAGL
                     ATPDQLLETAALPSAAGGARVAIRRRLTESPVLSTADLPEEYAEWWRRNRNREREWFR
                     DRFGLELELRAEGAVAVDPDDELTDVAFPGGGSAKHFALLLLGALVEAARARGDVAGH
                     AWTEISREEAEATSAEVFAAWSKWLKRDHRERPGLAHEDALALLASLGLVERAADGGL
                     RLHAAAARYSPQAVPGEDGPGEDCS"
BASE COUNT          395 a         1392 c         1222 g          448 t
ORIGIN      
        1 gccgcggcgg ccgccgcccg cgcctgcctg gcggcctcct cggcggcgcg cgccgcctgt
       61 tccgcccggg ccgacgcgac cgcgcaggcc ctcgcgtccg ccgtggcggc cagcaactcc
      121 ccgaacggcg gcaccgcgcc aagccacgcc tcgatggccg atcgcctgcc cctggcggcc
      181 tcctcggccg cggcggctgc gtcccgcgca tgcctggtcg cctccgctat ccggtctacc
      241 tcggccaggc gccgcgcccg ctcggccgct cgcgccgtcg cgccgatgta ctgggcggcc
      301 ggcttggcgg cgcggccggt caacgggccg agccgccagc gaccgtcggt ccccaccacc
      361 gcgccggtct cgtccgtctc gtccgtccca ttcggctcgc tcgtcccgcc gacgctgctg
      421 gccccgccga ccgcactggc cccggtcgct ccggtccccc cggtcccgca aggggcgatc
      481 ctggcgagca cgccgcggat ggtcgccgcg ctcaccgggc agtcgcctgg acagtccacg
      541 atcagcaggt ccgccaggct ccggcccggc actgccgggc cgagcggcaa caccacgtcg
      601 cgctgatctg ccccgagcac ctgcccgtcc acccggaccc aggcgtcgag caacccggag
      661 gactcgagcg ccgcctcgag ccctgcccgc tcggacgcgg cgcggtcccc cgcgaccgca
      721 agctcgcgca gcagcgggtc cgccgcctcg cgggcgatcc cgccgaggcg ctgcacggcc
      781 tgctcggtca gctcgtccgg gatctcgacg ggcacggcgc gatcgtcggc ccgccatgcg
      841 gccaggtcgg cctcgaacga ctcctgtgcc gagcgggcct gccgctcggc ggcggcgagc
      901 ctgacccgtt cgtcctgggc ggcgccctca agctcggcgg ccctttcctc cgctgacctg
      961 cgctgtgact ccgcccgctc ggcgccgcgc ctggcctcct cgaccacgcg gaccgccgcc
     1021 tgccgctcgc cgacccgcgg gcgggccgcg ccggctgccc tggcatgacc ggccagcgcc
     1081 tggtcgagca cgggcatgtc atcgagcatc gccgggtcga gcaggaccgc cgggacgatc
     1141 acgagcgcgg gcagcccgag cgcggcgaac tcggcggcgg actggccgga cgccgccagc
     1201 ccggtgacgt cacggcgcag gctggccgca tcgtgctcgg cccgctggcc ggccttgccc
     1261 gcccggcccc gggcgtcctg cgcgtggcgg cgggccttcc ccgcatgggc gaccagctcg
     1321 gcggcccgtt ccccgagcgc gagcacctgc tggtggctgc gggcctccgg gctgtcctcg
     1381 aggtccctgt gccgctgccg cgcccgcccg accgcggtgg ctgtggcgcg ctgttctccc
     1441 tcgttctccg cgatctgccg cgccacctcc gcgaggtcgg cggtgcgcct gccgatatcc
     1501 cgctccagcc tgctgagctc ccgggcggct gcgcgagcgc gccgccccgg gcggcggcga
     1561 cgccgcgcgc gtagccggcg tagagcccgg cgaaggcggt catggcctcg gacgcctgcc
     1621 ggcgcctggc gagctgctcg ccgaacgccg cgagctcgtc cagcgtcccg ccggcggacc
     1681 tgaccacgcc atcgtcgagt tcggggagcg cgacggacag gatttccgcg aggcggccag
     1741 gttcgatgtc ctcgccgacc tggggctggc gcagccagta cagcaggcgg agcagctcgt
     1801 cgtaccggcc gccgcccagc ccgaacaggg cttcgcccac gtgctgcttg tattgccgcg
     1861 cgctctcgaa gaaatggccg tcgtcctcga cggcggcgcg gcaccgttcc ctgggcagcg
     1921 gccccgcgcc gtcgtcgagc accaggtcgt gcccgacccg tcgcggcgag gtgaagtacc
     1981 aggacgaggc ggtcctggcg gactgggatg cgcgcacgcc gatcccgcag gtgaaccact
     2041 ccggctcgcc ggagtcggta atgtggacga actccaccca caggtagccg atccggctgg
     2101 ccccttcgta cccgtcggtc atcagccaca gcaggctggt gtggtgccgc ccggaggcgg
     2161 tcatcctgag cttgtcgccg tcgagcacga acggcagcag catctccagg gtcttcgatt
     2221 tgcccgcgcc gttggcgccg cgcaacagta gcctgccccc ggagaactcg aagacctgct
     2281 cgtcgtactg ccagacgttg agtatcccgg cccggctgag cctgaacctg tgcgggtgcg
     2341 catgcgcggc cgccagccgg gcgatccgtt gtgacgtcat gagcaatcct cgcccggccc
     2401 gtcctcgccg ggcacggcct gcgggctgta ccgcgcggcg gcggcgtgca gccgcagccc
     2461 gccgtccgcg gcccgttcca ccaggccgag cgaggccagc agcgccagcg cgtcctcgtg
     2521 ggccaggccc ggccgctccc tgtggtcccg cttgagccac ttcgaccacg ccgcgaacac
     2581 ctcggccgag gtggcctcgg cctcttcgcg tgagatctcc gtccaggcgt gcccggccac
     2641 gtcaccgcgc gccctggccg cctccacgag cgcgccgagc agcagcaggg cgaagtgctt
     2701 ggccgagccg ccgccgggga acgcgacgtc ggtgagctcg tcgtccgggt ccaccgcgac
     2761 cgccccctcg gcgcgcagtt cgagctcgag gccgaacctg tcgcggaacc actcgcgctc
     2821 cctgttgcgg ttgcgccgcc accactcggc gtactcctcg ggcagatcag cggtcgacag
     2881 gaccggcgac tcggtgagcc gccgcctgat cgccacccgg gcgccgcctg ccgccgacgg
     2941 cagcgccgcg gtctccagga gttggtccgg ggtggccaga ccggccagcg gcgcggagac
     3001 catgaggctc agccggtcgc ggcgcacgtc gagcagggtg gccgtccggc cgtcgctggc
     3061 ccaccgctcc aggtcaccgt cccgctcgcg caggacgccg tacctgatca gcgcggtgat
     3121 cgccgcgtag agggcgcgcc ggtccccgat ctggtcgagg tcgacgtcta ggccggcctc
     3181 ggccgcagcg gacctgatcg cggccacgag ctcgtccacg agcagctgcc cgcgggcctg
     3241 ggtcagcgcg gcgagggtga ggcagagcag agcgtaggca cgaggggtga acgcggcgcc
     3301 cccggccttt tcgagcgggc gggacgggtc gcgcccgagg ccgggcttga acagccgtgc
     3361 cgcgtgcgga tcgaccacca gccggtaccc gagcccgtcg gcgaacatcc tgaccagatc
     3421 accccggtgc ctgcggacga gcctgaactc ctcggcc
//
LOCUS       DASPYY010000760         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2093989, whole genome shotgun sequence.
ACCESSION   DASPYY010000760 DASPYY010000000
VERSION     DASPYY010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093989"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="thiE"
                     /locus_tag="VEP73_11880"
     CDS             complement(<1..529)
                     /gene="thiE"
                     /locus_tag="VEP73_11880"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYT27170.1"
                     /translation="MEAGRRAGRLAAARLYLCTPIRPDLAAFADAVLGAGVDVVQLRD
                     KEAEAVPLLEAAAVLREAADRHGALLAVNDRADVALAAGADVLHLGQDDLPMAWARRL
                     LGDGVLLGRSTHDLGQARRAVAEGWDYLVAGPVHPTATKPGRPPTGTGLLRAVAALDP
                     PVPWFAIGGIDLGTLD"
     gene            complement(529..1326)
                     /locus_tag="VEP73_11885"
     CDS             complement(529..1326)
                     /locus_tag="VEP73_11885"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYT27171.1"
                     /translation="MDDPLVIAGQRFTSRLVMGTGGASSLEALEQALLASGAGMATVA
                     LRRTSPDARGSVLDVLRRAGCRVLPNTAGCFTARDAVRTARLAREAFETDWVKLEVIG
                     DDRTLLPDAVELRDAAESLVDDGFTVLPYTSDDPVLARQLAELGCAAVMPLGSPIGSG
                     MGIRNPYNLRMILERATVPVLLDAGIGTASDAALAMELGCDGVLLASAVTRAREPALM
                     AEAMRKAVEAGRLAARAGRIPRRLYAEASTPFEGLADLEPDPAGLGG"
     gene            complement(1330..1536)
                     /gene="thiS"
                     /locus_tag="VEP73_11890"
     CDS             complement(1330..1536)
                     /gene="thiS"
                     /locus_tag="VEP73_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HYT27172.1"
                     /translation="MTVIVNGASTDVAPGATVAGVLAGLGHPVSGSGIAVALNGEVVP
                     RSAWDTTGLGDHDRLEVLGASQGG"
     gene            complement(1533..2681)
                     /gene="thiO"
                     /locus_tag="VEP73_11895"
     CDS             complement(1533..2681)
                     /gene="thiO"
                     /locus_tag="VEP73_11895"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HYT27173.1"
                     /translation="MVVAGGGVIGLGIAWRAALAGLAVTVVDATPGGGASWAAAGMLA
                     PVTEVHYGEEALLRLNLAAARRWPGFAGELEEASGRPVGYRPCGTLTVARDTDDLAAI
                     DEVYRFQSGLGLEVERLRSRECRQVEPGLAPGVRGGVLAAGDHQVDNRALVGALLAAA
                     GRAGVELRRARVEELRTGGDRVRGVTLAGGDALDAGTVVLAAGCWSSSVRGVPPSVLP
                     AVRPVKGQLLHLRGPADRPLCHHNVRGLDVYVVPREDGRVVVGATVEDQGFDTVVTAG
                     AVHGLLRDAFELLPDVTELELTETVAGLRPGSPDNAPLLGSSGLDGLVVATGHYRNGI
                     LLTPVTADAIAELLAGGPVPELIAPFSPRRFEERAASAGSARPGRSMA"
     regulatory      complement(2766..2878)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          338 a         1155 c         1134 g          319 t
ORIGIN      
        1 cgtccagggt gccgaggtcg atcccgccga tcgcgaacca cgggaccggc gggtcgaggg
       61 cggccacggc ccggagcagc ccggtgccgg tcggcggccg gccgggcttc gtcgcggtcg
      121 ggtggaccgg cccggcgacg aggtagtccc agccctcggc aaccgcccgc ctggcctgcc
      181 cgaggtcgtg cgtcgagcgg ccgagcagca ccccgtcgcc gaggaggcgc ctggcccagg
      241 ccatcggcag gtcgtcctgc ccgaggtgca ggacgtcggc gcccgcggcc agcgccacgt
      301 cggcccggtc gttgaccgcg agcagcgcgc cgtgccggtc ggccgcctcg cgcaggacgg
      361 cggccgcctc gagcagcggc acggcctcgg cctccttgtc gcggagctgc acgacgtcca
      421 cgccggcccc gagcaccgcg tcggcgaacg cggcgaggtc gggccggatg ggcgtgcaca
      481 ggtagagcct ggcggcggcc agccgcccgg cgcggcgccc ggcctccatc agccgcccag
      541 ccctgccggg tcgggctcca ggtcggcgag cccctcgaac ggggtggacg cctcggcgta
      601 gagccgccgg gggatccgcc cggccctggc ggcgagccgt ccggcctcga ccgccttgcg
      661 catcgcctcc gccatcaggg cgggctcccg cgcccgggtg accgcgctgg ccaggaggac
      721 cccgtcgcag ccgagctcca tcgccagggc ggcgtccgag gccgtgccga tcccggcgtc
      781 cagcagcacg ggcaccgtcg cccgctcgag gatcatgcgc aggttgtacg ggttgcggat
      841 ccccatgccg ctgccgatcg gcgagccgag gggcatcacg gcggcgcagc cgagctcggc
      901 gagctgcctg gccaggaccg ggtcgtcgct cgtgtagggc aggacggtga agccgtcgtc
      961 gaccagcgac tcggccgcgt cgcgcagctc gaccgcgtcg gggagcaggg tgcggtcgtc
     1021 gccgatgacc tcgagcttga cccagtcggt ctcgaacgcc tccctggcca gcctggccgt
     1081 gcgcaccgcg tcgcgggcgg tgaagcaccc ggccgtgttc ggcaggaccc ggcagccggc
     1141 ccggcgcagc acgtcgagga ccgacccacg ggcgtccggg ctggtgcggc gcagcgccac
     1201 ggtcgccatc ccggcccccg acgcgagcag ggcctgctcg agcgcttcca ggctcgacgc
     1261 cccgccggtg cccatgacga gcctcgacgt gaaccgctgg ccggcgatga cgagcgggtc
     1321 gtccatagtt cagcctccct ggctggcgcc gaggacttcc agccggtcgt ggtcgccgag
     1381 gccggtcgtg tcccacgccg accgcggcac cacctccccg ttgagcgcga ccgcgatgcc
     1441 ggacccggag accgggtggc cgaggccggc gagcacgccg gcgacggtgg cgcccggcgc
     1501 gacgtcggtg gacgcgccgt tgacgatgac ggtcatgcca tgctcctccc tggcctggcg
     1561 ctgccggcgg acgcggcccg ctcctcgaag cgccgcggcg agaacggggc gatcagctcg
     1621 gggaccggcc cgccggccag cagctcggcg atcgcgtccg cggtgaccgg ggtgagcagg
     1681 atgccgttgc ggtagtgccc ggtcgcgacg accagcccgt ccaggccgga ggagccgagc
     1741 aggggcgcgt tgtcgggcga cccgggacgc aggcccgcga cggtctcggt gagctcgagc
     1801 tcggtgacgt cgggcagcag ctcgaacgcg tcgcgcagca gcccgtgcac ggcgccggcg
     1861 gtgaccacgg tgtcgaagcc ctggtcctcg acggtggcgc cgaccaccac ccggccgtcc
     1921 tccctcggca ccacgtagac gtcgaggccg cggacgttgt ggtggcagag cggccggtcg
     1981 gcgggccccc gcaggtgcag cagctggccc ttgacgggcc ggacggcggg cagcaccgac
     2041 ggcggcacgc cccggaccga ggagctccag cagccggcgg cgagcaccac cgtcccggcg
     2101 tcgagcgcgt cgcccccggc gagcgtcacc ccgcgcaccc ggtccccgcc cgtgcgcagc
     2161 tcctccaccc gggctcgccg cagctcgacg ccggcccgcc ccgcggcggc gagcagcgcc
     2221 ccgacgaggg cgcggttgtc gacctggtgg tcgccggcgg ccagcacgcc gccgcgcacg
     2281 cccggggcga gcccgggctc cacctgccgg cactcccggc tccggaggcg ctccacctcg
     2341 agccccaggc ccgactggaa gcggtacacc tcgtcgatcg cggccaggtc gtcggtgtcg
     2401 cgggcgacgg tcagcgtgcc gcagggccgg tagccgaccg gccggccgct cgcctcctcc
     2461 agctcgccgg caaagccggg ccagcggcgg gcggcggcca ggttgagccg gagcagtgcc
     2521 tcctcgccgt agtggacctc ggtcacgggt gcgagcatgc cggccgccgc ccacgacgcc
     2581 ccgccgcccg gtgtggcgtc gaccacggtg acggcgagcc ccgcgagcgc cgcccgccac
     2641 gcgatgccga ggccgatcac ccccccgccg gccacgacca cgtcgggggc gtcccggcgc
     2701 ccggtgctgg gctgcggcga ggccgggtgg aggcgctgcg cgccggcggg cgccgcgcgg
     2761 tcccggtcgg tcaatgggct cctccctccg ccggcattac ccggtgcagg ttccaacggt
     2821 cggcgaccct ggcagccgcc ctctcagccc ggtggtccgg gctcccgtgt ggccccaggg
     2881 tacgcttcgc gggcggcccc gatcacggcg cgtcaggccg ggggcgggca ggcgtcaggc
     2941 cggggg
//
LOCUS       DASPYY010000761         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2516742, whole genome shotgun sequence.
ACCESSION   DASPYY010000761 DASPYY010000000
VERSION     DASPYY010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516742"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VEP73_11900"
     CDS             complement(<1..1251)
                     /locus_tag="VEP73_11900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYT27174.1"
                     /translation="MAGLLAAAVADIGGAERPGQVAMAKAVQRAIETGEHLAVQAGTG
                     TGKSLAYLVPAVRHAVTEQATIVVSTATIALQRQLIDRDLPRLAAVLEPALGRAPVFA
                     ILKGRRNYLCLHRVHSGAPEDPAEMLFDPRSVSATGRQVERLHEWAATTSTGDRDELV
                     PGVSEQAWRQVSVTARECIGAQRCPFGTRCYAELARDEAGRADIVVTNHALLAIDALE
                     DFDVLPEHDVVIIDEAHDLVDRVTAVGSAELSAAAVEIAARRCGRMVADAATGRLREA
                     AAAAERVLDEISYGRIDVLAEPLAVALAAVRDAAHGCATDLRASAKDNQEDVERVAAG
                     RSALAALDELHGAADRILGAFGADIASRPDVVWMDRPPAGEPSSRPPTLRVAPLEVGG
                     LLAERLFSRHTVTLTSATLSLGGSF"
     gene            1498..1818
                     /locus_tag="VEP73_11905"
     CDS             1498..1818
                     /locus_tag="VEP73_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT27175.1"
                     /translation="MAPLFEALSDATRRQVVQLLVAGPLRAGQLAAAAGTSAPAMSRH
                     LRVLLRAGIITDERLAQDARARLFRLRPQPIVALQAWLDQLQAHWNAQLQSFKWHVEG
                     RPER"
     gene            1815..>2215
                     /locus_tag="VEP73_11910"
     CDS             1815..>2215
                     /locus_tag="VEP73_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="HYT27176.1"
                     /translation="MSEGRSVSAEVEVAVDPPTAFSAFTQEMDLWWVRGPINFFDASR
                     AVAMTCEPGVGGRLLEVYDEAAGDALELGRITVWQPGERVCWQSSVDDVVVDVRFDPT
                     DGGTIVRVSATIPAGGTDRGGTSWVRVVPPW"
BASE COUNT          284 a          815 c          826 g          290 t
ORIGIN      
        1 gaacgagccg ccgagcgaca gcgtcgctga cgtcagcgtg accgtgtggc ggctgaacag
       61 ccgctccgcc agcagcccgc cgacctccag cggggcgacc cggagcgtcg gcgggcggga
      121 cgacggctcc ccggccggcg gccggtccat ccacaccacg tccggccggc tcgcgatgtc
      181 ggcgccgaac gcgccgagga tccggtcggc cgcgccgtgc agctcatcga gggccgcgag
      241 cgcggaccgg ccggccgcga ctcgttccac gtcttcctgg ttgtccttgg cgctggcccg
      301 caggtccgtc gcgcagccgt gggcagcatc acggaccgcg gccagcgcca cggccagcgg
      361 ctcggcgagg acgtcgatcc gcccgtagct gatctcgtcg agtacccgct cggctgccgc
      421 ggccgcctcc cggagcctgc cggtcgcggc gtcggcgacc atccggccgc agcggcgcgc
      481 ggcgatctcc accgcggccg cggacagctc ggccgacccg accgcggtga cccggtcgac
      541 caggtcatgc gcctcgtcga tgatcaccac gtcatgctcg ggcagcacgt cgaagtcctc
      601 gagcgcgtcg atcgcgagca gcgcgtggtt ggtgacgacg atgtccgccc ggccggcctc
      661 gtcgcgcgcc agctccgcat agcagcgggt gccgaacggg cagcgctgcg cgccgatgca
      721 ctcccgtgcg gtgaccgaga cctgccgcca ggcctgctcg ctgacgccgg gaacaagctc
      781 gtcgcggtcg ccggtggacg tcgtggccgc ccactcgtgc agccgctcga cctgccgccc
      841 ggtcgccgag accgagcgcg ggtcgaacag catctccgcc gggtcctccg gcgcgcccga
      901 gtgcacccgg tgcaggcaca ggtaattgcg gcggcccttg aggatcgcga agaccggcgc
      961 ccggccgagc gcgggctcga gcaccgccgc cagccgcggc aggtcgcggt cgatgagctg
     1021 gcgctgcagg gcgatcgtcg cggtcgacac cacgatggtg gcctgctcgg tcaccgcgtg
     1081 gcgcacggcc gggacgaggt aggcgagcga cttcccggtg cccgtcccgg cctggaccgc
     1141 cagatgctcg ccggtctcga tggcgcgctg gacggccttt gccatggcga cctgcccggg
     1201 ccgctcggcg ccgccgatgt cggcgacggc cgcggcgagc aggccggcca cgtccggcgg
     1261 gccggccttt cccgcggcct tgcggccggg cccggccgac ggcgggcggg catcatcggc
     1321 ggtgggctcg ggggcgcggc cggggggcac ccgccgacgc taccgcgccg gccgccgtga
     1381 tcgtgccacc gagccgggga cccgctggcc gggaccatca ccaaaaccag cttgacaatt
     1441 attaacgatt aacgcaatta ttgtcgcgtg gtcaccaaga cgccgatgga tcaggacgtg
     1501 gcaccgctgt tcgaggcctt gtccgacgca accaggcgcc aggtcgtgca gctgctcgtg
     1561 gcggggccgc tgcgcgccgg gcagctcgcc gccgccgcgg gcacgtctgc cccggccatg
     1621 agccggcacc tccgggtgct gctgcgggcc ggcatcatca cggacgagcg actcgcgcag
     1681 gacgcgcggg cccgcctctt ccggctccgg ccgcagccga tcgtggctct gcaggcctgg
     1741 ctcgatcagc tccaggcgca ctggaatgcc cagctgcagt cgttcaagtg gcacgtcgag
     1801 gggaggccag agcgatgagc gaaggcaggt cggtctccgc cgaggtcgag gtagcggtgg
     1861 atccgccgac ggcgttcagc gcgttcaccc aggagatgga cctgtggtgg gtgcgcgggc
     1921 cgatcaactt cttcgacgcc tcccgggctg tcgccatgac ctgcgagccc ggcgtcggcg
     1981 gccgcctcct cgaggtctac gacgaggccg cgggcgacgc gctggaactg ggccggatca
     2041 cggtgtggca gccgggcgag cgggtgtgct ggcaaagctc ggtcgacgac gtcgtcgtcg
     2101 acgtccggtt cgacccgacg gacggcggca cgatcgtccg ggtgagcgcc accatcccgg
     2161 ccggcggcac ggaccgcggc gggacgtcct gggtgcgggt ggtaccgccc tggtt
//
LOCUS       DASPYY010000762         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2377099, whole genome shotgun sequence.
ACCESSION   DASPYY010000762 DASPYY010000000
VERSION     DASPYY010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377099"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VEP73_11915"
     CDS             <1..711
                     /locus_tag="VEP73_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT27177.1"
                     /translation="DDVDLDVAVPGAAWAAYLNAGQVCTSAERFYVLEPVFDEFVDRL
                     VEETGKLRLGDPLADTTDVGPMVAEAQRARVERQLELARAGGARVLTGGDRGGFEHGW
                     YLSPAVVVDVDDAMDLMSIETFGPVAPVLRVGSLDEAISRANALPYGLGANVYTSRLD
                     YVLRCMEELEAGTVWFNDPLTDNDAGPFGGMKMSGNARELGPEGLESFRETKHVHLDA
                     RANRKPWWYPYGPGGYQG"
     gene            800..1483
                     /gene="lepB"
                     /locus_tag="VEP73_11920"
     CDS             800..1483
                     /gene="lepB"
                     /locus_tag="VEP73_11920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYT27178.1"
                     /translation="MDALRHAPTGATRLERYGGRHARVSSRHPRHARQRGLWSELPLL
                     VLTATVLAVLVKGFVVQAFFIPSGSMTPTLEVGDRVVVSRLAYRLDRPNHGDIVVFLR
                     PTARPRTAHSGALSWMRRAVAQGLGATPPGNEDLIKRVVGVPGDVLQGRGGRLWRNGR
                     PVKEPYLPGNVRTSDFGPVRIAPSHYWMMGDNREDSADSRVFGQVPGSALVGRAVARV
                     WPGARVGSL"
BASE COUNT          217 a          603 c          588 g          227 t
ORIGIN      
        1 gacgacgtgg acctcgacgt cgccgtgccc ggcgcggcct gggccgccta cctcaacgcc
       61 ggccaggtct gcacgtccgc cgagcggttc tacgtcctcg agccggtgtt cgacgaattc
      121 gtcgaccggc tcgtggagga gacggggaag ctgcggctcg gggatcccct ggccgacacc
      181 accgacgtcg ggccgatggt ggccgaggcg cagcgggcaa gggtcgagcg ccagctggag
      241 ctggcccgcg ccggcggggc gcgcgtcctc acgggcggcg accggggtgg cttcgagcat
      301 ggctggtacc tctcccccgc cgtggtcgtc gatgtcgacg acgccatgga cctcatgagc
      361 atcgagacgt tcgggccggt cgcgcccgtg ctgcgggtcg gctcgctcga cgaggccatc
      421 agccgggcga acgccctgcc ctacggcctc ggggcgaacg tgtacacgtc gcgcctcgac
      481 tacgtgctcc gctgcatgga ggagctcgag gcgggcaccg tctggttcaa cgaccccctc
      541 accgacaacg acgcggggcc gttcggcggc atgaagatgt ccggcaatgc ccgggagctc
      601 ggccccgagg gcctcgagtc gttccgcgag accaagcacg tccatctcga cgcgcgggcc
      661 aaccgcaagc cgtggtggta tccctatggg ccaggtggct accagggctg acggcgccag
      721 cccgccgagc ggtcctgtgt gcgttcccct tctggccggg gatgcgcgac aatgtccgcc
      781 cgtccgcacg ccgagaggag tggacgcgct gcgccacgcg ccgaccggag ccacccgcct
      841 cgagcggtac ggtggccggc atgcccgggt gtcgagccgc caccccaggc acgccaggca
      901 acggggcctc tggagcgagc tgccgctgct cgtgctcacc gccaccgtgc ttgccgtgct
      961 cgtcaagggc ttcgtggtcc aggcgttctt catcccatcc ggctcgatga cgccgacgct
     1021 cgaggtcggc gaccgcgtgg tcgtgagccg cctcgcgtac cggctcgacc gcccgaacca
     1081 cggcgacatc gtcgtgttcc tccgcccgac cgccaggccc cgcacggccc actccggggc
     1141 gctctcctgg atgcgccgcg cggtcgccca agggctcggc gccacccctc cgggcaacga
     1201 ggacctcatc aagcgggtgg tcggcgttcc aggcgacgtc ctgcagggca ggggcggccg
     1261 cctgtggcgg aacggccgcc cggtgaagga gccctacctg cccggcaacg tgcgcaccag
     1321 cgacttcggc ccggtcagga tcgcacccag ccactactgg atgatgggcg acaaccgcga
     1381 ggactcggcc gactcccgag tcttcggcca ggtgcccggc tcggcccttg tcgggcgtgc
     1441 ggtcgcacgg gtctggccgg gggcgcgcgt cgggagcctc tagggagacc cgcgatggcc
     1501 gcggcgagca cggacccggc gggggccgga ccggtcccga tgcccgtgcc cggcgacgac
     1561 tacccgcgca cctggctcga gttccaggag ttcttccccg acgaggaggc ctgcgcgcgc
     1621 tatctcgagc ggctg
//
LOCUS       DASPYY010000763         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1394077, whole genome shotgun sequence.
ACCESSION   DASPYY010000763 DASPYY010000000
VERSION     DASPYY010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394077"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1129)
                     /locus_tag="VEP73_11925"
     CDS             complement(11..1129)
                     /locus_tag="VEP73_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYT27179.1"
                     /translation="MARGLRLSEVGVRRARVHGQLLGEHDAGRDVAGVVSTVVGVQAQ
                     DLAAAAVCIWARAPDLRIGDVLAAAGRDPSIVLTWSLRGTRHLHHAADLRWLVGLVGP
                     VLNRPGRRAAQLGIAGDAGDRAVATVRAALSEGPLTRSEIKQRLAPLGIDVSGQAPIH
                     VLQRAGIEGILVILPDPHGEERYLLTDDWVPAARAIDPDEAPAMLASRYLAAYGPATP
                     EDFRAWSGLPSAAARQAWAAAAGEMAEIDGPAGRMWILEERRNAVLAAAGRPGPLRLT
                     GGFDTLLGGYRDRSLHLGPEQAPMVNPGGGLVKPTVVDDGRVVGTWRYLRERPHDRIE
                     VTLFRQVPRRDLAGEVAGVGRFLGTDPVLDLVAGPEAV"
     gene            complement(1146..>1566)
                     /locus_tag="VEP73_11930"
     CDS             complement(1146..>1566)
                     /locus_tag="VEP73_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYT27180.1"
                     /translation="EKLFGVGLVGASALAAAVVPLSTAYALAEAIGVERSVSRKFSEA
                     RLFLGLFTAQIALGVVLALKPGNLVHLLLATQVLNGLITPIILAFILILANRRSLLGD
                     AANGRVFRKVALLCVVGVGALSLVVVGQTVLGWIGVG"
BASE COUNT          230 a          587 c          569 g          180 t
ORIGIN      
        1 gctgtaacgc tcagacggcc tcgggacctg ccacgaggtc gaggaccggg tcggtcccca
       61 ggaagcgccc cacgcctgcc acctcgccgg caaggtcccg gcgcggcacc tgccggaaga
      121 gggtcacctc gatccgatcg tgggggcgct cccgcaggta gcgccaggtc cccacgaccc
      181 ttccgtcgtc gacgacggtg ggcttgacga ggcctccccc ggggttgacc atcggggcct
      241 gctcggggcc gaggtgcagg ctgcgatccc ggtagccgcc cagcagcgtg tcgaacccgc
      301 cggtcaggcg cagggggccg gggcggcctg ccgccgccag gacggcattg cgtcgctctt
      361 cgaggatcca catcctgcct gcggggccgt cgatctcggc catctctcca gcggcggcgg
      421 cccacgcctg ccgggcagcc gcggagggca gaccggacca ggcccggaag tcctccggcg
      481 tcgccggacc gtaggcggcc aggtaacggc tggccagcat cgccggcgcc tcgtccgggt
      541 cgatcgcccg ggcggccggg acccagtcat cggtgaggag gtagcgctcc tcgccgtgcg
      601 ggtccgggag gatgaccagg atgccctcga tgccggcgcg ctgcagcacg tggatcggcg
      661 cctgccccga gacgtcgatg cccagcgggg cgagccgctg tttgatctcg gatcgggtca
      721 ggggaccctc ggagagagcc gcgcgcacgg tcgccaccgc acggtcgccg gcatcgccgg
      781 cgatcccgag ctgggcggct cgcctcccgg gtcggttgag caccgggccg accagcccga
      841 cgagccaccg gaggtcggcg gcgtggtgga gatgccgggt cccccgcaag ctccaggtca
      901 gcacgatcga cgggtcccgg ccggccgcgg ccaggacgtc gccgatccgc aggtccgggg
      961 cacgcgccca gatgcagacc gcggccgccg ccaggtcctg ggcctgcaca ccgacgacgg
     1021 tggagacgac gcccgccacg tcccggcccg cgtcgtgctc gccgaggagc tggccgtgca
     1081 cgcgcgcccg gcgaaccccg acctcgctga gcctcagccc ccgggccacg tgctcggccc
     1141 tccgctcagc cgacgccgat ccatcccagc accgtctggc cgacgaccac cagcgagagc
     1201 gctccgaccc ccacgacgca gagcagcgcc accttccgga acacccggcc gttggccgca
     1261 tctccgagga ggctccggcg gttggcgagg atgagaatga aggccaggat gatcggcgtg
     1321 atcaggccgt tcagcacctg ggtcgcgagc aggaggtgga ccaggttgcc cggcttgagg
     1381 gcgaggacga ccccgagggc gatctgagcg gtgaacagcc cgaggaacag gcgcgcctcg
     1441 gagaacttcc tcgacaccga gcgctcgaca ccgatcgcct cggccagcgc gtacgccgtg
     1501 gagaggggca cgaccgcggc ggcgagggcc gacgcgccca ccaggcccac accgaagagc
     1561 ttctcc
//
LOCUS       DASPYY010000764         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_832344, whole genome shotgun sequence.
ACCESSION   DASPYY010000764 DASPYY010000000
VERSION     DASPYY010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832344"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1519
                     /locus_tag="VEP73_11935"
     CDS             239..1519
                     /locus_tag="VEP73_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYT27181.1"
                     /translation="MRGVWPQLVLVVILVGVNAAFAGSEVALISLREGQLQRLEEEGE
                     RGRLVARIARDPSQFLSTVQIGITLAGFLASAAAAVALAEPLVEPLGRFLGGAAEPVA
                     ILTVTALLTFFTLVFGELAPKRIALQRPERWALGAARPLAVVSTAMRPFVWLLSRSTD
                     LVVRLFGGDPRVQREEVTEEEVRDLVASQATFSPQHRTIIAGAFEIADLRLREVLVPR
                     RDVVALRADLPAAAGLRQLVGQTHTRAPVYRDDLDDVLGVVHLPDLVYASGQVGDHAR
                     PAVTLPETMEVLDALRRLQEQRQQLALVINEYGGTEGIVTLEDLLEEVVGEIYDEFDP
                     DVATVDRQDDGSFVLPGSFPFHDLPDLGIELPEGTYATVAGFVLERLGRIPTGGEVVE
                     ADGWRIEVLAVERRAITRVRIRPLERGQRTGETS"
     gene            complement(1554..2549)
                     /locus_tag="VEP73_11940"
     CDS             complement(1554..2549)
                     /locus_tag="VEP73_11940"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HYT27182.1"
                     /translation="MTEHSPDLGRPRRRRPLRAVLQALVVAAFALFIVYAVAKRWDGV
                     RDYAGRLSVAAIGLSTTAVLGAIWCSFLCWKAVLADFGSRVPVGGGMRIFFVGQLGKY
                     LPGKLWPILTQTRLGAEYGVPKRSSGAAAGVFMLLVLGTGLVIGVATLPFLGAQAFHR
                     YWWALAVLPAALVAMHPAVLNWGLGLGLRLARREPMPQPLTLRGTATAAFWCLPMWAL
                     FGVHTWALALDLGGGGLPLLLRSTGVFAASWSIGFLLLVAPAGAGPREAAFIVLLGAT
                     LPVAQATVIALVSRLLITVGDVAWGLLALLGARRRQPGRTDTVAPPPPARETVRD"
     gene            complement(2546..>3272)
                     /locus_tag="VEP73_11945"
     CDS             complement(2546..>3272)
                     /locus_tag="VEP73_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27183.1"
                     /translation="AARLRDAGCVAAFVAERRLDEFAASLGPRPGRPALVAVGAGLLG
                     RTGAPHSPGGATHTPGGGVAALDFSRVVPGMGDAFDGGATSVADDALLPGDRPPGNPL
                     TQGALLAAAGEAASGLGVGATDRLFAGLPASTALGVAAGLVAPFLVGAGVVVEQAFGP
                     STFWKRVADERVAVALLAAGQVEALLGADGDLGGLDLGRLRSVACELDRVPRALALAW
                     ERRFTVPISPVPAGAPLAAGTKR"
BASE COUNT          446 a         1214 c         1255 g          357 t
ORIGIN      
        1 gccggccggt acgtcaagga cctgggcaac acccccggac acctgaacca ctggtcgaag
       61 tcgggcttcg tcaagttcgt ctccaccgtg gccgacgtgc gcgaggtgac ctcgccgttc
      121 ccgtggacga cggtctgggc gacgctgccc ctgtagaccc ggcgcctccc gccccgcgcc
      181 acggcgcacc ggaggatact ggccgcccgg tggtgcccga cgcgcgtgag ggtgtgcgat
      241 gagaggggtg tggccgcagc tcgtcctcgt ggtcatcctg gtgggggtga acgccgcctt
      301 cgcaggcagc gaggtcgccc tgatctcgct gcgggagggg cagctccagc ggctcgagga
      361 ggagggggag cgcggccggc tcgtcgcccg gatcgcgaga gacccgagcc agttcctctc
      421 caccgtccag atcggcatca ccctggccgg cttcctcgcc tcggcggcgg ccgccgtggc
      481 gctcgccgaa ccactggtcg agccgctcgg gaggttcctc ggcggcgccg ccgagccggt
      541 cgcgatcctg accgtcaccg ccctgctcac cttcttcacg ctcgtcttcg gcgagctggc
      601 cccgaagcgc atcgcgctgc agcggcccga gcggtgggca ctgggggcgg cccggccgct
      661 cgccgtggtc tcgaccgcca tgcggccgtt cgtctggctg ctgtcgcgct cgaccgacct
      721 cgtggtgcgc ctgttcggcg gggacccgag ggtgcagcgt gaggaggtga ccgaggagga
      781 ggtccgcgac ctcgtggcgt cccaggccac cttcagcccc cagcaccgca cgatcatcgc
      841 gggggcgttc gagatcgcgg acctgaggct cagggaggtg ctggtgccaa ggcgcgacgt
      901 cgtcgccctg cgcgccgacc tgcccgcggc cgcggggctg cggcagctcg tcgggcagac
      961 ccacacccga gcgccggtct accgcgacga cctcgacgac gtcctcggcg tcgtgcacct
     1021 gcccgacctc gtctacgcga gcggccaggt cggcgaccac gcccgcccgg ccgtgaccct
     1081 ccccgagacg atggaggtgc tcgatgcgct gcggcggctg caggagcagc gccagcagct
     1141 cgcccttgtg atcaacgagt acggcggcac cgagggcatc gtcaccctcg aggacctgct
     1201 cgaggaggtg gtcggggaga tctacgacga gttcgacccg gacgtggcga cggtcgaccg
     1261 tcaggacgac ggctcgttcg tcctgcccgg gtccttcccg ttccacgacc tccccgacct
     1321 cggcatcgag ctgcccgagg gcacctacgc gacggtggcc gggttcgtcc tcgagcgcct
     1381 cggccggatc ccgaccggcg gcgaggtggt cgaggcggac ggctggcgga tcgaggtgct
     1441 cgcggtcgag cgccgggcga tcacccgggt ccggatacgt ccgctcgagc gcgggcagcg
     1501 gaccggcgag acgagctgac gcgccggcgc ccggcaggcg cctggggtcg cggtcagtcg
     1561 cgcacggtct cgcgggccgg tggcgggggt gcgaccgtgt ccgtgcggcc gggctggcgc
     1621 cgccgggcgc ccaggagggc gagcagtccc caggcgacgt ccccgacggt gatgagcagg
     1681 cgcgacacga gcgcgatcac ggtggcctgc gcgaccggca gggtggcgcc gagcaggacg
     1741 atgaacgccg cctcccgggg accggccccg gccggggcga ccagcagcag gaagccgatc
     1801 gaccaggagg ccgcgaacac gccggtcgag cggaggagca gcggcaggcc gcctccgccc
     1861 aggtcgaggg cgagcgccca ggtgtggacg ccgaacagcg cccacatggg caggcaccag
     1921 aacgcggccg tggcggtccc gcgcagggtc agcggctgcg gcatcggctc gcgccgcgcg
     1981 aggcgcaggc cgaggccgag tccccagttc agcacggccg ggtgcatcgc gacgagggcg
     2041 gcgggcagca cggcaagcgc ccaccagtag cggtggaacg cctgggcgcc gaggaagggg
     2101 agcgtggcga cgccgatgac gagcccggtg ccgagcacga ggagcatgaa caccccggcc
     2161 gccgcgcccg aggaccgctt cggcaccccg tactcggccc cgagccgggt ctgggtgagg
     2221 atcggccaga gcttgcccgg caggtacttg ccgagctggc cgacgaagaa gatccgcatg
     2281 ccgccgccca cgggcacgcg cgagccgaag tcggccagca cggccttcca gcagaggaag
     2341 ctgcaccaga tggcgccgag cacggccgtg gtcgacaggc cgatcgccgc gaccgacagc
     2401 cggccggcgt agtcgcgcac gccgtcccag cgcttcgcca ccgcgtagac gatgaagagc
     2461 gcgaacgccg ccacgacgag cgcctgcagg acggcccgca gcgggcggcg ccggcgcggg
     2521 cgcccgaggt cgggcgaatg ctccgtcacc gtttcgtccc cgccgccagg ggcgccccgg
     2581 cgggcacggg ggagatgggc accgtgaagc ggcgctccca ggcgagcgcg agcgctctcg
     2641 gcacgcggtc gagctcgcac gcgaccgacc gcaggcgacc gaggtcgagt ccgccgagat
     2701 ccccgtccgc tccgagcagc gcctcgacct gcccggccgc cagcagggcg acggccaccc
     2761 gctcgtcggc gacgcgtttc cagaacgtgg agggaccgaa cgcctgctcc accaccacgc
     2821 cggccccgac caggaacggt gcgaccaggc cggcggccac gccgagggcc gtggaggcgg
     2881 gcagcccggc gaacagccgg tccgtggcgc ccacgccgag gcccgaggcg gcctcgcccg
     2941 ccgcggccag cagggcgccc tgggtgagcg ggttgcccgg cgggcggtcg ccggggagca
     3001 gggcgtcgtc ggcgaccgac gtggccccgc cgtcgaacgc gtcgcccatg cccggcacga
     3061 cgcgggagaa gtccagggcc gcgaccccgc cgccgggcgt gtgcgtggcg ccgcctggac
     3121 tgtgcggggc gccggtccgg ccgagcaggc cggcgccgac ggccacgagc gcgggccgtc
     3181 cgggccgggg gccgaggctc gcggcgaatt cgtccaaccg ccgctcggcc acgaacgcgg
     3241 ccacgcagcc ggcgtcccgc agccgggccg cc
//
LOCUS       DASPYY010000765         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2095515, whole genome shotgun sequence.
ACCESSION   DASPYY010000765 DASPYY010000000
VERSION     DASPYY010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095515"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VEP73_11950"
     CDS             <1..1077
                     /locus_tag="VEP73_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYT27184.1"
                     /translation="PLEAIVLVGGQGTRLRPLTLSAPKPLLPTAGVPFLAHLLARAAV
                     GGVTHVVLATCYKADMFASVLGDGAAFGLSIDYVQEERPLDTGGGIRNAASSLRGGGA
                     ADPIVVLNCDILSGHDLRAQVDLHTKTDAAVTLHLTEVEDPSPFGCVPTDETGRVTAF
                     LEKTPDPVTNRINAGCYVFRRDVLEEIPSGRRVSVERETFPGLIEAGALVMGYLETAY
                     WLDVGTPQAFVRGSCDLVLGRLPAPAVPGPSGPRLVLANAFVADGAEVSGGTTVGAGA
                     RIDAGATVSGCVLFDDAMVGEGAVVRDSILGRGAIVAPGAVLDDAVIGDGAYVGAGNE
                     LARGIRVWPGTRLEPTSVRFSSDV"
     gene            1082..>1645
                     /locus_tag="VEP73_11955"
     CDS             1082..>1645
                     /locus_tag="VEP73_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27185.1"
                     /translation="MTAAPSAETGPSAVSDAGLVREWPLPFSLDVPLTLSVHRRGNGD
                     PAYATEAGGAVWRTSLTPDGPGTLRVTSRRAPVTGRACVTGRACVTGRAWGPGAGWLL
                     DRLPGWLGFHDDRAGFTARHPAVGELALRYEGLRVGRSGRVFEALVPAVLEQKVVSSE
                     AHRAWRFLLLKYGEPAPGPAPAGMRVVP"
BASE COUNT          178 a          611 c          632 g          224 t
ORIGIN      
        1 ccacttgagg cgatcgtcct tgtcggaggc cagggcaccc ggctgcggcc gctgaccctg
       61 tccgcgccga agcccctgct gcccaccgcg ggcgtgccgt tcctggcgca cctgctggcc
      121 cgggcggcgg tcggcggcgt cactcacgtg gtcctggcga cctgctacaa ggcggacatg
      181 ttcgcctcgg tcctggggga cggggcggcg ttcgggctgt cgatcgacta cgtgcaggag
      241 gagcggccgc tggacaccgg cggcggcatc cgcaacgcgg cctcgtccct gcgcggcggc
      301 ggcgccgcgg acccgatcgt ggtgctgaac tgcgacatcc tctccggcca cgacctgcgc
      361 gcgcaggtcg acctgcacac caagaccgac gccgcggtga ccctgcacct gaccgaggtg
      421 gaggacccgt ccccgttcgg ctgcgtgccg accgacgaga ccggccgggt gaccgcgttc
      481 ctggagaaga cgccggaccc ggtgaccaac cggatcaacg ccggctgcta cgtgttccgc
      541 cgcgacgtcc tcgaggagat cccgtccggg cgccgggtgt cggtggagcg ggagacgttc
      601 cccgggctca tcgaggccgg ggcgctcgtc atgggctacc tcgagacggc ctactggctc
      661 gacgtgggga cgccgcaggc gttcgtgcgc ggctcctgcg acctggtgct cgggcggctg
      721 cccgccccgg ccgtgccagg gcccagtggc cccaggctgg tgctggcgaa cgcttttgtg
      781 gccgacggcg cggaggtgtc cggcgggacc acggtgggcg cgggcgcgag gatcgacgcc
      841 ggggcgaccg tatccggctg cgtgctcttc gacgacgcca tggtgggcga gggcgcggtg
      901 gtgcgcgact ccatcctcgg ccgcggcgcc atcgtcgccc cgggcgccgt gctcgacgac
      961 gcggtgatcg gcgacggagc ctacgtcggc gccggcaacg agctcgcccg cggcatccgg
     1021 gtatggcccg gtacccgcct cgagccgacc tcggtccgct tctcctccga cgtgtgacgg
     1081 catgaccgcg gccccgagcg cggaaacggg gccgagcgcg gtctccgacg cggggctggt
     1141 ccgcgagtgg ccgctgccgt tcagcctgga cgtcccgctc accctgtcgg tgcaccggcg
     1201 cggtaacggg gatcccgcct acgccaccga agcgggcggc gcggtctggc gcacctcgct
     1261 gaccccggac ggcccgggca ccctccgggt cacctcccgc cgcgcgcccg tgaccgggcg
     1321 ggcctgcgtg accgggcggg cctgcgtgac cgggcgggcc tggggccccg gcgcgggctg
     1381 gctgcttgac cgcctgcccg gctggctcgg cttccacgac gaccgggcgg ggttcaccgc
     1441 ccgccacccg gcggtcggcg agctggcgct gcggtatgag ggcctgcgcg tcgggcggag
     1501 cggccgggtc ttcgaggcgc tggtacccgc ggtcctcgag cagaaggtgg tcagctccga
     1561 ggcccaccgc gcctggcgct tcctgctgct caagtacggc gagcccgcgc cgggcccggc
     1621 gccggccgga atgcgcgtgg tcccg
//
LOCUS       DASPYY010000766         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1957063, whole genome shotgun sequence.
ACCESSION   DASPYY010000766 DASPYY010000000
VERSION     DASPYY010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957063"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1500)
                     /locus_tag="VEP73_11960"
     CDS             complement(136..1500)
                     /locus_tag="VEP73_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYT27186.1"
                     /translation="MSQVNADVVLGGRYRLLERIATGGMGTVWKAEDTVLGRRVAVKV
                     LSEALADDAAFVDRFRREARASAGLAHPNVASVFDYGEDGSAGRPGHVAFLVMELVEG
                     ETLASALHRLGRLPPDEAARIAAEVAAALEAAHRSGLVHRDVKPANVMITPRGEVKVM
                     DFGIAAAVGASSLTATGSVIGTAAYVSPERVRGERATPSSDVYSLGVVLFEMLTGRTP
                     FEAETPVGLALAHVQERAPSVVDLAPGVPAPLAEACELALSKDPHDRPRSAAEFRSML
                     LRVARTPPAAPAPATAPAAGDATEVIEPDGGTAVLPAVGGPPPPTRRRRRVRPWIVTG
                     AVAALLLTAFAWILFVAPPTATTRPPSSPSGPQPSVSATSPAGVPVPDVHGLSLDQAR
                     NALVAAGLTPGPVQEVSGPKDVVVGTLPPAGTTVGSTTPVTLLVGGKGHGDNGKHDGK
                     GNGG"
     gene            complement(1511..>3092)
                     /locus_tag="VEP73_11965"
     CDS             complement(1511..>3092)
                     /locus_tag="VEP73_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYT27187.1"
                     /translation="AAGAGLGDRAEGARRPTGPGVDGHAVGPRGDQGRPAVIDRQIRR
                     LGLAFVILFLLLFGQVNYLQVFAAQRISENPANRRIILQEYSTQRGAILARDERTVLA
                     KSVPTNDSLKYLRVYPGGALYGQITGYYSLIYGRSGLESAYDDYLSGRASALQPQNLV
                     DEILGRAKRGATVVTTIDPKLQQAAAHALGSLPGAVVALDPRTGEVLAMVSNPSFDPS
                     PLASHIGANQRAAWNRLTHSPSKPLLARAFQEVYPPGSTFKMVTASAALQNGLMPTTL
                     FPNPPLLDLPQTTNTLQNFGGEHCLGGVPQITLAQALTVSCNVVFGEVGLRLGADKLV
                     AQAQRFGFGQHVPFDLPFAEGQIPPASSFKDNLPLVAYSAIGQASVAANPLQMAMVGE
                     AIANGGVEMQPHLVRRILDPQGRVVNTTLPQVYARPISPQTAAQMTSMMVQVVQSGTG
                     TAAQIPGVAVAGKTGTAQHAGGNNPHAWFVCFAPAQNPRIVVAVVVLNGGSMGSEATG
                     GQVAAPIAKAVLQAALGK"
BASE COUNT          424 a         1141 c         1110 g          417 t
ORIGIN      
        1 actgcgaccc gagcaccttc acggcgaccc ggcgacctag gacctggtcc gtgccgaggt
       61 agacctcggc catgccgcct tggcccagca gcgagtcgag ctcgtagcgc cccgcgagga
      121 cgcgcccctc aggggtcaac cgccgttgcc cttgccgtcg tgtttgccgt tgtccccatg
      181 ccccttcccg ccgaccaaca aggttacggg ggtcgtggac cccacggtgg tacccgcggg
      241 cggaagcgtc ccgacgacga cgtccttcgg acccgagacc tcctggacgg gaccgggcgt
      301 gagacccgcc gcgaccaggg cgttccgggc ctgatccagc gacaacccat gcacgtcggg
      361 aacgggcacc ccggccggtg aggtcgcaga gacggacggc tgggggccac tgggcgacga
      421 cggaggcctc gtggtggcgg tcgggggcgc gacgaacagg atccaggcga acgccgtcag
      481 gagcagcgcc gccaccgctc cggtgacgat ccagggtcgg acacgtcgcc ggcggcgggt
      541 cggcggcggc ggcccgccca ccgccgggag caccgcggtc ccgccgtccg gttcgatgac
      601 ctcggtggcg tcgccggcgg ccggcgccgt cgccggcgct ggcgccgccg gcggggtccg
      661 agcgacgcga aggagcatcg acctgaactc ggccgcgctc ctcggccggt cgtgggggtc
      721 cttcgacagg gcgagttcgc acgcctcggc gagcggcgcg ggcacgcccg gcgcgaggtc
      781 aacgaccgac ggcgcccgtt cctgcacgtg ggccagcgcg aggccgaccg gggtctcggc
      841 ctcgaagggc gtccggccgg tgagcatctc gaagagcacc acgccgagcg agtacacgtc
      901 ggaggagggt gtggcccgtt cgccgcgcac ccgttcgggc gacacgtagg ccgcggtccc
      961 gatcacgctg cccgtcgcgg tgagcgagct ggcgccgacc gccgcggcga tgccgaagtc
     1021 catcaccttg acctcgcccc gcggggtgat catcacgttg gcgggtttca cgtcccggtg
     1081 gacgagcccc gagcggtggg cggcctcgag cgcagccgcc acctccgccg cgatccgggc
     1141 tgcctcgtcc ggaggcagcc ggcccaggcg atgcagcgcc gacgcgagcg tctcgccttc
     1201 caccagctcc atcaccagga aggccacgtg gccgggccgg ccggcgctgc cgtcctcacc
     1261 gtagtcgaac acgctcgcca cgttggggtg cgccaggccc gccgacgccc gggcctcgcg
     1321 acggaaccga tccacgaacg ccgcgtcgtc cgccagcgcc tcggacagca ccttcacggc
     1381 cacccggcgg ccgagcacgg tgtcctcggc cttccacacc gtccccatgc cgcccgtggc
     1441 gatccgctcg agcaaccggt accgtccccc gagcaccacg tcggcgttca cctgcgacat
     1501 cgttcctcca tcacttcccc aaagcggcct ggagtaccgc cttcgcgatg ggcgcggcga
     1561 cctgtccgcc ggtggcctcg ctccccatgc tgccgccgtt caggacgacg acggccacga
     1621 cgatccgcgg gttctgggca ggagcgaagc acacgaacca cgcgtgcggg ttgttccccc
     1681 cggcgtgctg ggccgtgccc gtcttgccgg ccaccgccac cccggggatc tgggctgccg
     1741 tccccgtgcc cgactgcacg acctggacca tcatgctggt catctgggcc gcggtctgcg
     1801 gcgagatcgg tcgcgcgtag acctgcggga gggtcgtgtt caccacgcgg ccctgcgggt
     1861 ccaggatccg gcggaccagg tgcggctgca tctccacccc gccgttcgcg atggcctcgc
     1921 cgaccatcgc catctgcagc gggttggccg ccacgctggc ctgcccgatg gccgagtacg
     1981 ccaccagcgg caggttgtcc ttgaacgacg acgccggcgg gatctgcccc tccgcgaagg
     2041 ggaggtcgaa cggcacgtgc tgaccgaagc cgaaccgctg cgcctgggcc accagcttgt
     2101 ccgcgcccag ccgcagcccg acctcgccga acacgacgtt gcaggagacc gtcagcgctt
     2161 gggccagcgt gatctggggg acgccgccga ggcagtgctc gccgccgaag ttctggagcg
     2221 tgttcgtcgt ctggggcaga tcgagcagcg gcgggttggg gaacaacgtg gtgggcatca
     2281 ggccgttctg gagggcggcc gaggccgtga ccatcttgaa ggtcgagccg ggcgggtaca
     2341 cctcctggaa cgcccgggcg aggagcggct tcgacggcga gtgcgtcagg cggttccacg
     2401 cggcccgttg attcgccccg atgtgcgacg ccagcgggct cgggtcgaac gacgggttcg
     2461 acaccatggc cagcacctcg ccggtccgag ggtccagggc caccacggcg ccggggagac
     2521 tgccgagggc gtgcgccgcg gcttgctgca gcttcgggtc gatcgtggtc acgacggtcg
     2581 ccccccgctt ggcgcgaccc aggatctcgt ccaccaggtt ctggggctgc agggccgacg
     2641 cccgccccga caggtagtcg tcgtacgcgg actccagccc gctccggccg tagatcagcg
     2701 agtagtaccc ggtgatctgg ccgtacagcg cgccgcccgg atagacccgc aggtacttga
     2761 gcgagtcgtt cgtcggcacg ctcttggcca ggacggtccg ctcgtcgcgg gcgaggatcg
     2821 cgccgcgctg ggtgctgtac tcctgcagga tgatccggcg gttcgccggg ttctcggaga
     2881 tgcgctgggc ggcgaacacc tgcaggtagt tcacctggcc gaacagcagc aggaacagga
     2941 tcacgaaggc gaggccgagc cgccggatct gccggtcgat cacggcgggc cgcccctggt
     3001 cgccacgggg tccgacggcg tgcccgtcga cgccggggcc cgtggggcgg cgcgctccct
     3061 ccgcccggtc gccgagaccc gcaccagcag cg
//
LOCUS       DASPYY010000767         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2236269, whole genome shotgun sequence.
ACCESSION   DASPYY010000767 DASPYY010000000
VERSION     DASPYY010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236269"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..>1969
                     /gene="dxs"
                     /locus_tag="VEP73_11970"
     CDS             234..>1969
                     /gene="dxs"
                     /locus_tag="VEP73_11970"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HYT27188.1"
                     /translation="MLESIAQPSDLRQLSQAELAGLAAEIREFLVRAVAARGGHLGPN
                     LGVVELTIALHRAVDSPVDQIVWDTGHQAYVHKLLTGRQADFASLRTMGGLSGYPSRS
                     ESPHDVIENSHASTALGYALGLAEARRAGRGAGRVVAVVGDGSLTGGVALEALNLIGH
                     RKPDMLVILNDNGRSYAPTVGGLAGHLGPLRLHPSYERLKKGVEETLGRVPLFGDGMV
                     EAAKRAKEGAKALLAPRVVFEDLGWQYAGPVDGHDLPALERAIDHAKRVSGPVLLHVV
                     TQKGRGYKPAEEHDEDNLHSLGAFDPETGRALPRSGDDVPFTDVFGQALLDEARRHPE
                     LVAISAAMLGPTKLSVMAQEFPDRVYDVGIAEQLGVTMAAGLALRGLRPVCAIYSTFL
                     QRAFDQVMMDVSLHRLPVIFAIDRSGITGEDGSSHHGVYDVTYLREIPNMVIASPRDG
                     NELRRALATAVAYTGGPVAIRIPRSTTPKLTVSGPVRKTRIGAWEVRSRGSDVLLLGL
                     GKLAVTCEEAARLLKQDGIRVTLVDPRWVKPLDPRLGALAGSHRLVATVEDNVLAGGF
                     GSAVAEVLADEGV"
BASE COUNT          264 a          760 c          686 g          259 t
ORIGIN      
        1 ggggaggccg tcgggcgggc cgcgctgctg gccggctacc cggccgccct cgccgccctc
       61 gccgtgctcg gcgcggccgg cggcgtgcag ctcctgctgg ccgcgtggcg cgcgcccgcc
      121 gacgcccagg accccgacgc ccaggacccc gacacccagg accccgacac ctaggatacc
      181 taggtgaagt tccggaggca gaatccagcc gaggtgtcta tgctcaagtg gtcatgctcg
      241 agtccatcgc ccagccaagc gacctccgtc agctctcgca ggcggagctc gccgggctcg
      301 ccgccgagat ccgggagttc ctcgtccgcg cggtcgcggc ccgcggcggc cacctcggcc
      361 ccaacctcgg cgtggtcgag ctcaccatcg cgctccaccg ggccgtcgac tcccccgtcg
      421 accagatcgt gtgggacacg ggccaccagg cctacgtcca caagctcctg accggcaggc
      481 aggccgactt cgccagcctg cgcacgatgg gcggcctctc cggctacccg tcgcgcagcg
      541 agagccccca cgacgtgatc gagaactcgc acgcgtcgac ggcgctcggc tacgcgctcg
      601 gcctcgccga ggcgcgccgc gccggccgcg gcgccggccg ggtcgtcgcc gttgtgggcg
      661 acggctcgct caccggcggg gtcgccctcg aggcgctcaa cctcatcggc caccgcaagc
      721 ccgacatgct cgtgatcctg aacgacaacg gccgctccta cgcgcccacg gtcggcgggc
      781 tcgccgggca cctcggcccg ctccggctcc accccagcta cgagcggctc aagaagggcg
      841 tcgaggagac cctcggccgg gtgccgctgt tcggcgacgg catggtcgag gcggccaagc
      901 gtgccaagga gggcgccaag gcgctgctcg ccccccgggt ggtgttcgag gacctcggct
      961 ggcagtacgc cgggccggtg gacggccacg acctgcccgc cctggagcgg gccatcgacc
     1021 acgccaagcg cgtttccggg ccggtcctgc tccacgtggt cacccagaag gggcggggct
     1081 acaagccggc cgaggagcac gacgaggaca acctccactc gctcggcgcg ttcgaccccg
     1141 agaccggccg ggcgctgccc cggtcaggcg acgacgtgcc cttcaccgac gtgttcggcc
     1201 aggcgctgct cgacgaggcc cggcgccacc ccgagctggt cgcgatctcg gccgcgatgc
     1261 tcgggccgac caagctcagc gtgatggccc aggagttccc cgaccgggtc tacgacgtcg
     1321 gcatcgccga gcagctcggg gtgacgatgg cggccggcct cgccctgcgc gggttgcgcc
     1381 cggtctgcgc catctactcc accttcctgc agcgggcgtt cgaccaggtg atgatggacg
     1441 tctccctcca caggctgccg gtcatcttcg cgatcgaccg gtcgggcatc accggcgagg
     1501 acggctcctc ccaccacggc gtctacgacg tcacctacct gcgcgagatc ccgaacatgg
     1561 tgatcgcgtc cccgcgcgac gggaacgagc tgcggcgcgc cctcgccacc gcggtcgcct
     1621 acacgggcgg cccggtcgcg atccgcatcc cgcggtcgac caccccgaag ctgaccgtct
     1681 ccggcccggt gcgcaagacc aggatcggcg cctgggaggt gcgctcgaga ggctccgacg
     1741 tgctgttgct cgggctcggc aagctcgccg tgacctgtga ggaggcggcc aggctgctca
     1801 agcaggacgg catcagggtg accctggtcg accccaggtg ggtgaagccg ctcgacccgc
     1861 ggctcggcgc gctggccggc tcccaccggc tggtcgccac cgtggaggac aacgtgctcg
     1921 ccggcgggtt cggctcggct gtggccgagg tgctcgccga cgagggtgt
//
LOCUS       DASPYY010000768         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2377977, whole genome shotgun sequence.
ACCESSION   DASPYY010000768 DASPYY010000000
VERSION     DASPYY010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377977"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VEP73_11975"
     CDS             <1..246
                     /locus_tag="VEP73_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HYT27189.1"
                     /translation="VASFESEFDVKGLDGGPVSAVFIRAPWISGQGPAVEVLAEVDGR
                     VVAAREGNLLATSFHPELAGEHRFHRWFADLVRDAHR"
     gene            297..1100
                     /locus_tag="VEP73_11980"
     CDS             297..1100
                     /locus_tag="VEP73_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT27190.1"
                     /translation="MSGHSKWATIKHQKGAKDAKRGKLFARLIRGIEVAARVGGGNPE
                     GNPTLADAIQKARDNSVPNDTIDRAVKRGAGEAGGLAPDLVTYEGYAPGGVAVLVEAL
                     TDNRNRAAADIRHAFSKAGGSLAEAGSVAYLFTRKGELYVAGAAGAADQGAADQGAAD
                     RVAADRIEDRVMEVALEAGAEDVEATGDGFRVTTAPGDLRAVRAAFDTAGILYDSAEV
                     TMVPSVSVPLERDAAVKVLRLLDALEDLDDVQDVYANFDIPDAVMAEVG"
     gene            1366..2007
                     /gene="ruvC"
                     /locus_tag="VEP73_11985"
     CDS             1366..2007
                     /gene="ruvC"
                     /locus_tag="VEP73_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYT27191.1"
                     /translation="MRVVGVDPGLGRCGYAVLDSAAGPLAAVAYGTIATRGGQVAPRL
                     AELAERFRLVVAETRPDCLAIERLFVNANVRTAMTVGQASGVVLLVAAERGLPVASYT
                     PSQVKRAVTGTGTAPKEQVGYMVRAALGLAATPTPADTADAIAVALCHLQHMGMAAHG
                     GRAATAIGPAAAADWLARAAPTSPGLRPAGPPRPARPAGRGVGRLPGPNGRRP"
     gene            2004..2609
                     /gene="ruvA"
                     /locus_tag="VEP73_11990"
     CDS             2004..2609
                     /gene="ruvA"
                     /locus_tag="VEP73_11990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYT27192.1"
                     /translation="MIASLRGRLAELLPDGAVVEVGGVGYKVFLTPKALASLHRGADV
                     LVHTVTYVREDALALYGFVTGDERRAFEQLLGASGVGPKLALAILSVHSPDALRRALT
                     AGDADALTLVPGVGRKGAARLILELRGRLGGGEPEVPADDGTRPAYAEVREALGALGY
                     GPAEVKVALEALPADAGELPTEELLRLALRALAGPAARGAR"
     gene            2609..>3299
                     /gene="ruvB"
                     /locus_tag="VEP73_11995"
     CDS             2609..>3299
                     /gene="ruvB"
                     /locus_tag="VEP73_11995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYT27193.1"
                     /translation="MTDILAPQAMPGEGAGEGSLRPRRLAEFVGQARTREQLGIVLES
                     ARRRGLPPDHLLFSGPPGLGKTSLATIVAAELEVGFRITSGPALERAGDLAAILTGLE
                     ADDVLFVDEVHRLPRAVEEVLYPAMEDFQIDVVLGKGPGARSLRLDLPRFTLVGATTR
                     TGLITSPLRDRFGFSARLDLYRPEELQAIVTRSAGILGVRVDAAGAAVLARRSRGTPR
                     IANRLLRRVRDY"
BASE COUNT          400 a         1261 c         1235 g          403 t
ORIGIN      
        1 gtcgcctcgt tcgagtccga gttcgatgtc aagggcctgg acggcggtcc ggtgtcggcc
       61 gtgttcatcc gggctccctg gatctccggg caggggccgg cggtcgaggt gctggcggag
      121 gtcgacggcc gcgtcgtcgc cgcgcgcgag ggcaacctcc tcgcgacctc cttccatccc
      181 gagctggccg gcgagcaccg gttccaccgc tggttcgccg acctggtccg cgacgcgcac
      241 cgatagccgc cggccgggcc gggggccagg ccttggacga cccgaaggga gcaggcatgt
      301 ccggccactc gaagtgggcc acgatcaagc accagaaggg cgccaaggac gccaagcggg
      361 gcaagctgtt cgcccggctg atccgcggca tcgaggtggc cgcccgcgtc ggcggcggca
      421 acccggaggg caacccgacg ctggccgacg cgatccagaa ggccagggac aactcggtcc
      481 cgaacgacac gatcgaccgg gcggtcaagc gcggcgcggg cgaggccggc ggcctggcgc
      541 ccgacctggt cacctacgag ggctacgccc caggcggcgt cgccgtgctc gtcgaggcgc
      601 tgaccgacaa ccgcaaccgc gccgccgccg acatccgcca cgccttctcc aaggccgggg
      661 gcagcctcgc ggaggccggc agcgtcgcct acctgttcac ccgcaaggga gagctgtacg
      721 tggccggcgc cgcgggcgcc gcggaccagg gcgccgcgga ccagggcgcc gcggaccggg
      781 tcgccgcgga ccggatcgag gatcgggtca tggaggtcgc cctcgaggcg ggcgcggagg
      841 acgtcgaggc gacgggcgac ggcttcaggg tcaccaccgc ccccggggac ctgcgggccg
      901 tccgggccgc gttcgacacg gcgggcatcc tctacgactc ggccgaggtg accatggtgc
      961 cgagcgtgtc ggtgcccctc gagcgcgacg ccgcggtcaa ggtgctccgc ctgctcgacg
     1021 cgctcgagga cctcgacgac gtccaggacg tctacgcgaa cttcgacata cccgacgccg
     1081 tcatggccga ggtgggctga ccgtcggggg cggggcgtcg cctcagatgc ggcggcgcct
     1141 cacgaggaac atgaccaggg cgatgatggc gagcacgatg agaatcagga gcagcgtcct
     1201 catgagagcc tcccttgccg tcggacaggt ccggatcgta ccagtgtgct tgtacccttc
     1261 tgggccgctc cgctaacggc cgccgtcgag ggcatggttg agcccgctgg cgggaaccgg
     1321 taggctgccc tccaaacacc tgttcgagct gggaggctgg ccgggatgcg ggtggtcggg
     1381 gtcgaccctg ggctcggccg ttgcgggtat gcggtgctcg actcggccgc cgggccgctc
     1441 gcggcggtcg cctacggcac catcgccacc cgaggcggcc aggtcgcgcc aaggctggcc
     1501 gagctggccg agcggttccg cctcgtcgtg gcggagacgc ggccggactg cctggcgatc
     1561 gagcgcctgt tcgtcaacgc caacgtgcgc accgccatga ccgtcggcca ggcgtccggg
     1621 gtcgtcctgc tggtggccgc cgagcggggg ctgccggtcg cgtcgtacac gccgtcgcag
     1681 gtcaagcggg cggtgaccgg cacgggcacg gcacccaagg agcaggtcgg ctacatggtc
     1741 cgggccgccc tcggcctcgc ggccaccccg accccggccg acaccgccga cgcgatcgcg
     1801 gtggccctct gccacctcca gcacatgggg atggcggccc acggcggccg ggcggccacg
     1861 gccatcgggc cggcggcggc cgccgactgg ctcgcccgcg ccgccccgac ctcgcccggg
     1921 ctccggcccg ccgggccgcc gcgccccgcc cggcccgccg ggcggggcgt ggggcggctc
     1981 cccgggccga acgggaggcg gccatgatcg cgagcctgcg cggccgcctc gccgagctcc
     2041 tgcccgacgg ggcggtggtc gaggtcggcg gcgtcggcta caaggtcttc ctcaccccga
     2101 aggcgctcgc ctcgctgcac cgtggcgccg acgtgctcgt gcacacggtc acctacgtcc
     2161 gcgaggacgc cctcgcgctg tacgggttcg tgaccggcga cgagcggcgc gccttcgagc
     2221 agctcctcgg cgcctccggg gtcggcccca agctcgccct ggccatcctc tcggtgcact
     2281 ccccggacgc gctgcggcgc gcgctgaccg ccggtgacgc cgacgccctc accctcgtgc
     2341 ccggggtcgg ccggaagggc gcggcgcgcc tcatcctcga gctcaggggc aggctcggcg
     2401 gcggcgagcc cgaggtgccg gccgacgacg gcacccggcc ggcctacgcc gaggtgaggg
     2461 aggcgctcgg cgcgctcggc tacgggcccg ccgaggtcaa ggtcgcgctc gaggcgctgc
     2521 ccgccgacgc cggcgagctg cccaccgagg agctcctccg cctggccctg cgcgccctgg
     2581 ccgggcccgc cgcccgcggt gcccgctgat gacggacatc ctggcgcccc aggcgatgcc
     2641 cggcgagggc gccggcgagg gcagcctccg cccgcgccgg ctcgccgagt tcgtcgggca
     2701 ggcgcggacc cgcgagcagc tcggcatcgt gctcgagtcg gcccgccggc gcgggctgcc
     2761 ccccgaccac ctgctgttct ccgggccgcc gggcctcggc aagacgagcc tggccacgat
     2821 cgtcgccgcc gagctggagg tcggcttccg gatcacgtcc ggcccggccc tcgagcgggc
     2881 cggcgacctc gccgcgatcc tcaccggcct ggaagcggac gacgtgctct tcgtcgacga
     2941 ggtgcaccgg ctgcccaggg cggtcgagga ggtcctctac ccggcgatgg aggacttcca
     3001 gatcgacgtg gtcctcggca aggggccggg ggcgcgcagc ctccgcctcg acctgccccg
     3061 gttcacgctc gtcggcgcga cgaccaggac cggcctgatc acctcgccgc tgcgtgaccg
     3121 gttcggcttc tccgcccgcc tcgacctcta ccggcccgag gagctgcagg ccatcgtgac
     3181 ccggtccgcg ggcatcctcg gggtccgggt cgacgccgcc ggggccgccg tgctcgcccg
     3241 ccgctcccgg gggaccccca ggatcgcgaa ccggctgctc cgccgggtcc gcgactacg
//
LOCUS       DASPYY010000769         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_832746, whole genome shotgun sequence.
ACCESSION   DASPYY010000769 DASPYY010000000
VERSION     DASPYY010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832746"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VEP73_12000"
     CDS             <1..527
                     /locus_tag="VEP73_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27194.1"
                     /translation="DLLDAPGKLLIWDPPDHMRPIPAKAYHLDRARIPRVLTAAGAGA
                     VPDLDPATATGIAATPGRGDRTGPDPTTGPVPDRPRQPAPPSPSADTVPIPAPSQDGD
                     PLAALRTCLRAAGPGGAKVAELASAIGRAKTWVYERLQDLGRTGEVERAGHGRWRLTH
                     PHPDDRDSEDGHDG"
     gene            664..849
                     /locus_tag="VEP73_12005"
     CDS             664..849
                     /locus_tag="VEP73_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27195.1"
                     /translation="MARTCRRCGWPKHAHACLLAHARNPAVRGWSRREWARYRASGRL
                     PWWDHTSRRDRRRIRGR"
     gene            994..>1576
                     /locus_tag="VEP73_12010"
     CDS             994..>1576
                     /locus_tag="VEP73_12010"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYT27196.1"
                     /translation="MLDDGAQGAQTVATKDEARALVAEKRLQFTRDTWQGRRRGRLPF
                     STWADEWWADWSADPDRSPNTLATTESRLRLHVRPWFGDRPIEKIGPAEVRRWQTQLA
                     AKTGRATLAQCRSLALRIFQYAMDEGAIEANPVRKVPAPKRRADPDKVLGKAKRRALT
                     PEEAGRLLARFPLFWWDHVIALLGTGLRFGELAG"
BASE COUNT          214 a          675 c          523 g          164 t
ORIGIN      
        1 ccgacctgct ggacgcgccc gggaagctgc tcatctggga cccgcccgac cacatgcgcc
       61 ccatccccgc caaggcctac cacctcgacc gcgcccgcat cccccgcgtc ctcaccgccg
      121 ccggcgcggg cgccgtgccc gacctcgacc ccgccaccgc caccgggatc gccgcgacgc
      181 ccggtcgggg cgaccggacc ggacccgacc cgaccaccgg tcccgtcccc gaccgtcccc
      241 gccagccggc cccaccctcg ccgtccgccg acaccgtccc catccctgct ccgtcccagg
      301 acggggaccc gctcgccgcg ctgcggacct gcctgcgcgc cgccggcccg ggcggcgcca
      361 aggtcgccga gctggccagc gccatcggcc gcgccaagac ctgggtctac gaacgcctcc
      421 aagacctcgg ccgcaccggg gaggtggagc gcgccgggca cggccgctgg cgtctcaccc
      481 acccccaccc cgacgaccgg gacagcgagg acggccacga tgggtgaccg ccgcggctcg
      541 cgcgtgcgcg cgcgtcacgc ctgcgcgttg ggccaccacc ccgcccgtcc ggacgtccgg
      601 acggaccacc accccgcgtg gccgacccgg ccgcgcccga gcgagcggaa ggaggtgaca
      661 cccatggccc gcacctgccg acgctgcggc tggcccaagc acgcccacgc ctgcctgctc
      721 gcccacgccc gcaaccccgc cgtccgcggc tggtcccgcc gcgaatgggc ccgctaccgc
      781 gcaagcggcc ggctcccctg gtgggaccac accagccgcc gcgaccgccg ccgcatccgc
      841 ggccggtagc cgtcccacga ccctcgggag ggtgggccgt cccaccgtcc ccgtcctccc
      901 cggccctcga tcgatcggag cgccatggca tcggcaggca tccgcaagcg cgtcagcggg
      961 cgcacgggtc gggtgagcta ccaggtctgg tggttgcttg atgacggcgc ccagggtgcc
     1021 cagaccgttg ccaccaagga cgaggcgcgc gccctcgtgg ccgagaagcg gttgcagttc
     1081 acacgcgaca cctggcaggg acgacgccgg ggccggctgc cgttcagcac ctgggcggac
     1141 gagtggtggg ccgactggtc ggccgacccc gaccgcagcc ccaacacgct cgccacgacc
     1201 gagagtcgcc tacggctgca cgtgcgaccc tggttcgggg atcgtcccat cgagaagatc
     1261 ggcccggccg aggtccgccg ctggcagacc caactcgccg ccaagaccgg ccgcgccacg
     1321 ctggcgcaat gccgatcgct ggcgctgcgc atcttccagt acgccatgga cgaaggcgcg
     1381 atcgaggcca acccggtccg caaggtgccc gcgcccaagc gccgtgccga ccccgacaag
     1441 gtgctcggca aggccaagcg ccgggcgctc accccggagg aagccggccg gctgctcgcc
     1501 cggttcccgc tgttctggtg ggaccacgtc atcgccctgc tcggcaccgg cctgcggttc
     1561 ggggagctgg ccgggc
//
LOCUS       DASPYY010000770         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_832808, whole genome shotgun sequence.
ACCESSION   DASPYY010000770 DASPYY010000000
VERSION     DASPYY010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832808"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VEP73_12015"
     CDS             <1..640
                     /locus_tag="VEP73_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYT27197.1"
                     /translation="ATVTAALVAIGLLGVWDDSLDTLSQVIAAALVTVVIGVAIGILT
                     SRSDTLEKVVRPVLDAMQTMPAFVYLIPVIGLFGIGRTPAAIAAVIYALPLVIRLTDS
                     GIRQVPAVTKEAAISSGASPRQLLWKVELPLARPAIMLGVNQGIMMVLAMVIIGALVG
                     GGALGYDVLVGLSQNQFGLGIAAGATIVCLGIVLDRITQAAARAEGRTHTHH"
     gene            750..1754
                     /locus_tag="VEP73_12020"
     CDS             750..1754
                     /locus_tag="VEP73_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYT27198.1"
                     /translation="MRKTRIGRTMRLAALAAVLVLLVAACGGAKSTSGGGVGGGSGGG
                     SGSGGGKTVRLAVNSWVGSQADVAVACWLIQNKLSSKCDQVTIDEVPTWPAMGQNRLE
                     GVLEVWGHEDLYAQYVTKAKKVVDGGKLGATGHIGWYVPTYVMQQHPELATWQGLKTN
                     WKLFQTAESGGQGQLLDGSPSYVTEDAALAKNLGLNLKVIYAGSEAAQITAIRKAYAA
                     KKPLLFYWYTPQWLNSQLQFSEVKLPSRTEGCDATAGKKPDKTNCAYPTYPLYKAFSA
                     EFASGNSKAYQLLKSFQLTNKQQDDVAALIADKGMKPDAAAAQWAQQNQSVWQPWISS
                     "
     gene            1801..>3966
                     /locus_tag="VEP73_12025"
     CDS             1801..>3966
                     /locus_tag="VEP73_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT27199.1"
                     /translation="MGNGRRVVVVGAGIVGCSVADHLTQLGWHDVTVVDQGPLPATGG
                     STSHAPGLVFQTNPSQTMTGFAQYSVRRYGELRRDGRSCFDPVGSIEVARTPERWTDL
                     ARKHGLATSWGVPSQLLSAGQVAERLPLLDPATVLGGFHVPTDGLARAVWAAGAMAEL
                     ARSRGAVLTGGTRVTGITVDGGRVRAVRTSRGDLDADVVVSCAGMWGPLVGRMAGVRV
                     PLLAFQHQYARTTPVPGLAGATEEATHPILRDQDRAMYFRQHQDRYGIGSYQHVPLPV
                     RPEDILDPADAPVMPSLMPFTPNDFKQAWADAQELLPALRGTEVAESLNGLFSFTPDG
                     LPLVGESRDVPGFWVAEAVWITHAAGVGKATAEWIAEGAPGTDLRECDLHRFDAFARS
                     PAYVRRRAVQQYDEVYDVIHPQQPMEEPRPLRVSPFHRRQEELGAVFLEAGGWERPQW
                     YEANAALAAGRPAPAPGAWAARYWSPVVAAEHHATRDRVAMYDMTPLKRAVVSGPGAP
                     GLLERLTTGRLDRPPGYVTYALMLDERGGILSDVTVARLGEQRFQVGCNGPRDLDRLL
                     RHAPPDGSVHVEDTTGGTCCIGLWGPLARDVVQGLSEDDFSDAGFGFFRAREVDVGEV
                     PVTALRLSYVGELGWELYTSADLGLRLWDLLWRAGRPHGVVAGGRGAFNGLRLEKGYR
                     AWGTDMWSEHDPDEAGLGFAVRLDKGDFVGRAALERRRAA"
BASE COUNT          550 a         1472 c         1418 g          526 t
ORIGIN      
        1 ggccacggtc acggccgcgc tcgtcgcgat cgggctgctc ggcgtgtggg acgacagcct
       61 cgacacgctc tcgcaggtca tcgcggccgc gctggtcacc gtcgtcatcg gggtcgccat
      121 cgggatcctc acctcgcgca gcgacacgct cgagaaggtg gtgcgcccgg tcctcgacgc
      181 gatgcagacc atgccggcgt tcgtctacct catcccggtc atcggcctgt tcgggatcgg
      241 ccggacgccg gccgcgatcg cggccgtgat ctacgccctg ccgctcgtca tccgcctgac
      301 cgactcgggc atccgccagg tgccggccgt gaccaaggaa gcggcgatct cgtccggcgc
      361 gagcccccgg cagctcctgt ggaaggtcga gctgccgctg gcccgcccgg cgatcatgct
      421 cggcgtcaac caggggatca tgatggtcct cgccatggtc atcatcggcg ccctggtcgg
      481 cggcggcgcg ctcggctacg acgtgctggt cggcctgtcg cagaaccagt tcgggctcgg
      541 catcgcggcc ggcgcgacca tcgtgtgcct gggcatcgtc ctcgaccgca tcacccaggc
      601 ggcggcccgg gccgagggcc gtacgcacac ccaccactga cgtgtccgcg gcggggcgcc
      661 gccgcgccgc ctcgacgacg gttcctctcg acccaaggac tcgacaccca ggactcgaca
      721 cccaaggact ccacccaagg agggtcccca tgcgcaagac aaggatcgga cggaccatgc
      781 ggctcgccgc cctcgccgcc gtcctggtgc tgctggtcgc cgcctgtggc ggcgccaagt
      841 cgaccagtgg cggcggggtg ggcgggggct ccggcggtgg gagcggctcc ggcggcggca
      901 agaccgtgcg gctcgccgtc aactcctggg tcggttccca ggccgacgtc gccgtcgcct
      961 gctggctgat ccagaacaag ctgagctcca agtgcgatca ggtcaccatc gacgaggtgc
     1021 cgacctggcc ggccatgggc cagaaccggc tcgagggcgt gctcgaggtc tgggggcacg
     1081 aggacctcta cgcccagtac gtcaccaagg cgaagaaggt cgtcgacggc ggcaagctcg
     1141 gcgccaccgg ccacatcggc tggtacgtgc cgacgtacgt catgcagcag cacccggagc
     1201 tggccacctg gcagggactc aagaccaact ggaagctgtt ccagaccgcg gagagcggcg
     1261 gccagggaca gctgctcgac ggctcgccga gctacgtgac cgaggacgcc gcgctggcga
     1321 agaacctcgg cctcaacctc aaggtcatct acgccggctc cgaggcggcc cagatcaccg
     1381 ccatccgcaa ggcgtacgcg gccaagaagc cgctgctgtt ctactggtac accccgcagt
     1441 ggctgaactc gcagctgcag ttctccgagg tcaagctccc ctcgcgcacg gaaggctgcg
     1501 acgcgacggc cggcaagaag ccggacaaga ccaactgcgc ctacccgacc taccccctgt
     1561 acaaggcgtt cagcgccgag ttcgccagcg gcaactccaa ggcctaccag ctgctcaaga
     1621 gcttccagct gaccaacaag cagcaggacg acgtggccgc cctgatcgcc gacaagggca
     1681 tgaagccgga cgcggccgcg gcgcagtggg cccagcagaa ccagtccgtc tggcagccat
     1741 ggatctcgtc gtgagcgccg tttagaatga tggcgttccc ctgagagaaa cggttgcggt
     1801 atgggcaacg gcaggcgggt cgtggtcgtc ggcgcgggca tcgtcggctg cagcgtcgcc
     1861 gaccacctca cgcagctcgg ctggcacgac gtgaccgtcg tcgaccaggg ccccctgccc
     1921 gccacgggcg gctcgacctc ccacgcgccg ggcctcgtct tccagacgaa cccgtcgcag
     1981 accatgaccg ggttcgccca gtactcggtg cggcgctacg gcgagctgcg gcgggacggg
     2041 cggtcctgct tcgacccggt cggcagcatc gaggtggccc gcacccccga gcgctggacg
     2101 gacctggccc gcaagcacgg gctggccacc tcctggggcg tgccgtcgca gctgctctcc
     2161 gccgggcagg tggccgagcg gctcccgctg ctcgacccgg cgaccgtgct cggcggcttc
     2221 cacgtcccga ccgacgggct ggcccgggcc gtctgggcgg ccggcgccat ggccgagctc
     2281 gcgcgctcgc gcggcgcggt gctcacgggc ggcaccaggg tcaccggcat cacggtcgac
     2341 ggcgggcggg tcagggcggt gcggacctcc cggggcgacc tcgacgccga cgtcgtcgtg
     2401 agctgcgccg gcatgtgggg cccgctggtc gggcgcatgg cgggcgtgcg cgtccccctg
     2461 ctggccttcc agcaccagta cgcgcgtacc acgcccgtgc cggggctcgc cggggcgacc
     2521 gaggaggcca cccaccccat cctgcgcgac caggaccgcg ccatgtactt ccggcagcat
     2581 caggaccgct acgggatcgg ctcctaccag cacgtcccgt tgccggtccg gcccgaggac
     2641 atcctcgacc cggcggacgc cccggtgatg ccgtcgctca tgcccttcac ccccaacgac
     2701 ttcaagcagg cctgggccga cgcccaggag ctgctcccgg cgctgcgcgg caccgaggtc
     2761 gccgagtcgc tgaacgggct gttctcgttc acgcccgacg gcctgccgct cgtcggggag
     2821 tcgcgggacg tccccgggtt ctgggtcgcc gaggcggtct ggatcaccca cgcggccggg
     2881 gtcggcaagg ccacggccga gtggatcgcc gagggcgccc caggcaccga cctgcgcgag
     2941 tgcgacctgc accgcttcga cgccttcgcc cgcagccccg cctacgtccg caggcgcgcg
     3001 gtgcagcagt acgacgaggt ctacgacgtc atccacccgc agcagccgat ggaggagccc
     3061 aggcccctgc gggtcagccc cttccaccgc cggcaggagg agctcggcgc ggtcttcctg
     3121 gaggcgggcg gctgggagcg cccgcagtgg tacgaggcga acgccgccct cgccgcgggg
     3181 cggcccgcgc cggctccggg ggcgtgggcg gcacgctact ggtcgccggt tgtcgcggcc
     3241 gagcaccatg cgacccggga ccgcgtggcc atgtacgaca tgacgccgct caagcgggcg
     3301 gtcgtcagcg gccccggcgc gcccggcctg ctcgagcggc tcaccaccgg ccggctcgac
     3361 cgcccgccag ggtacgtcac ctacgcgctg atgctcgacg agcggggcgg gatcctgagc
     3421 gacgtcacgg tcgcgcgcct gggcgagcag cgcttccagg tcggctgcaa cgggccccgc
     3481 gacctcgacc ggctgctgcg ccacgccccg ccggacgggt cggtgcacgt ggaggacacg
     3541 accggcggca cctgctgcat cgggctgtgg ggcccgctgg cccgcgacgt ggtccagggg
     3601 ctgagcgagg acgacttctc cgacgccggg ttcggcttct tccgggcgcg ggaggtggac
     3661 gtcggcgagg tgccggtcac cgcgctgcgg ctgtcgtacg tgggcgagct cggctgggag
     3721 ctgtacacct cggccgacct cggcctgcgc ctctgggacc tcctgtggcg ggcgggccgg
     3781 ccgcacggcg tcgtcgcggg cgggcggggc gccttcaacg gcctccgcct cgagaagggg
     3841 taccgcgcct ggggcaccga catgtggagc gagcacgacc ccgacgaggc cgggctcggc
     3901 ttcgccgtcc ggctcgacaa gggcgacttc gtcggccggg ccgcgctcga gcggcgccgg
     3961 gccgcc
//
LOCUS       DASPYY010000771         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_127801, whole genome shotgun sequence.
ACCESSION   DASPYY010000771 DASPYY010000000
VERSION     DASPYY010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127801"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VEP73_12030"
     CDS             <1..232
                     /locus_tag="VEP73_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27200.1"
                     /translation="ERFAVSPGHGRFYPVVPTRFTDEGPYVEPGDLLGEVRNGAASIP
                     VRSPFRGWVMAHLAWEGELVTPGQILLSLRPF"
     gene            293..1411
                     /locus_tag="VEP73_12035"
     CDS             293..1411
                     /locus_tag="VEP73_12035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYT27201.1"
                     /translation="MPAEIVGLGTAVPRYVLTNADLERLVDTNDQWITERTGIKERRI
                     AGRGESTSTLGRLAAERALASAGLGPDDVDLLVVTTASPDYPLPSAACILQLELGMQR
                     GAAMDVIAGCAGFVYGLATASQFVASGAVGTALVVGAETLSRICDYSDRSTCILFGDG
                     AGAAVLRRGERGIRGFSLGSDGARSRLLMTPGGGSRFPATARTVAERRHYIVMEGREV
                     FKGAVTAMAASSLEAIRGSGLEPGDIDLVIPHQANQRIIEATARRLKVPMTDVVMNIA
                     RYGNTSAASIPIAMAEAWEQGQIRAGQRLLLTAFGAGLAWASLVLDWTLDPPAAPSPE
                     EPTESALPIVEGLRDGTLWDGLEMLPPLPGATQAEVAG"
     gene            1408..>2198
                     /gene="fabG"
                     /locus_tag="VEP73_12040"
     CDS             1408..>2198
                     /gene="fabG"
                     /locus_tag="VEP73_12040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYT27202.1"
                     /translation="MTAGPEGQAPRAPQGAGTPQGAGRVPVRRRGLEGRTALVTGASG
                     AIGRACAVALAGEGARLVVAYGSDEQGAQETAGLVKAAGAEVVCVQADLADPAAPKEL
                     VAAAGDQGVDILVNNAGMTRDGLLLRMRDQDFTDVLDLNLVASFRCTREAIRGMLRRR
                     WGRVVSISSVVGLVGNPGQANYAAAKAGLIGFTMSVAREVAQRGITANVVAPGYIPSK
                     LTDAMNEEAKQATLSAIPAGRLGSPEDVAAAVRFLAGEDASYITG"
BASE COUNT          295 a          757 c          859 g          287 t
ORIGIN      
        1 ggagcggttc gccgtctccc ccgggcatgg ccgcttctac ccggtggtgc cgacccggtt
       61 caccgacgag gggccctacg tggagccggg tgacctgctc ggggaggtcc gcaacggcgc
      121 ggcgagcatc ccggtccgca gcccgttccg cgggtgggtt atggcccacc tggcatggga
      181 aggcgagctg gtgacccctg ggcagatcct gctcagcctc cgtccgttct agcgttcgag
      241 atcgagtaca ttccgggtcg tccgaccgtc caggccggcg aaaggagctg tcgtgcccgc
      301 cgagatcgtg ggattgggaa ccgccgttcc ccgctacgtc ctgaccaacg cggacctcga
      361 gcgactggtc gacaccaacg accagtggat caccgagcgc accgggatca aggaacgccg
      421 gatcgcgggc agaggcgagt ccaccagcac cctcggcagg ctcgcggccg agcgggcgct
      481 cgcgtcggcc gggctcgggc ccgacgacgt cgacctgctg gtggtgacga ccgcgtcccc
      541 ggactacccg ctgccgtcgg ccgcctgcat cctgcagctg gagctcggga tgcagcgggg
      601 cgcggcgatg gacgtgatcg cgggctgtgc cgggttcgtg tacgggctgg cgacggcgtc
      661 gcagttcgtg gcgtcgggcg cggtcggcac ggccctggtg gtcggggccg agacgctgag
      721 ccggatctgc gactacagcg accggtcgac ctgcatcctg ttcggcgacg gagccggggc
      781 ggcggtcctg cggcgcggcg agcggggcat ccggggattc tcgctgggct ccgacggggc
      841 gcggtcgagg ctgctgatga cccccggagg gggctcccgc ttcccggcca ccgccaggac
      901 cgtcgcggag cggcgccact acatcgtcat ggagggccgg gaggtgttca agggggcggt
      961 gaccgcgatg gccgcgtcca gcctcgaagc gatcaggggc tcgggcctcg agccgggcga
     1021 catcgacctc gtcatccccc atcaggccaa ccagcggatc atcgaggcca ccgcgcggcg
     1081 cctgaaggtg cccatgaccg acgtggtgat gaacatcgcg aggtacggca acacgtcggc
     1141 cgcctcgatc ccgatcgcga tggcggaggc gtgggagcag ggccagatcc gggccgggca
     1201 gcggctgctg ctcaccgcgt tcggggctgg gctcgcgtgg gcgtcgctcg tgctggactg
     1261 gacgctcgac ccccccgcgg cgccgagccc ggaggagccc acggagtcgg cgctgccgat
     1321 cgtcgagggg ctgcgcgacg ggacgctgtg ggacgggctg gagatgctgc cgccgctccc
     1381 gggcgccacc caggcggagg tcgcgggttg accgccgggc cggagggcca ggccccccgg
     1441 gcgccccagg gcgccgggac gccccagggc gccggacgcg tcccggtgcg gcgccggggc
     1501 ctcgagggac ggaccgccct cgtgacgggc gcgtcagggg cgatcgggcg ggcgtgcgcc
     1561 gtggcgctgg ccggggaagg tgcgcggctg gtggtcgcct acggctcgga cgagcagggc
     1621 gcgcaggaga ccgccggcct ggtcaaggcg gccggtgccg aggtcgtctg cgtccaggcc
     1681 gacctggccg acccggccgc gcccaaggaa ctggtcgcgg cggccggcga ccagggggtc
     1741 gacatcctgg tgaacaacgc cggcatgacc agggacgggc tgctgctccg catgcgggac
     1801 caggacttca ccgacgtgct cgacctgaac ctggtggcgt cgttccgctg cacgcgggag
     1861 gcgatccgtg ggatgcttcg gcgccgctgg ggcagggtgg tgtcgatctc gagcgtcgtc
     1921 ggcctggtag gcaacccggg gcaggcgaac tacgccgcag ccaaggccgg gctcatcggc
     1981 ttcaccatga gcgtggcccg tgaggtcgcc cagcgcggga tcaccgccaa cgtggtcgcc
     2041 cccggctaca tcccgtccaa gctgaccgac gccatgaacg aggaggcgaa gcaggcgaca
     2101 ctgtcggcca taccggccgg gcggctcggc agcccggagg acgtcgccgc ggccgtgcgc
     2161 ttcctggccg gcgaggacgc ctcctacatc accgggca
//
LOCUS       DASPYY010000772         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1816360, whole genome shotgun sequence.
ACCESSION   DASPYY010000772 DASPYY010000000
VERSION     DASPYY010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816360"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VEP73_12045"
     CDS             complement(<1..1368)
                     /locus_tag="VEP73_12045"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HYT27203.1"
                     /translation="MAVTSDPQAVAAGRPAPLWRPSRERVLRSNLVRYEHWLSEHRGL
                     DFHAYDDLWRWSVTELDAFWTSIWEFFGVGDGRAPSPALAERRMPGARWFPGAELSYA
                     EHALRRRDDHPALVFGSEDGTLGTVTYAELHRQVAAAAAGLRRLGVGRGDRVVAYLPN
                     VPETVVAFLASASLGAIWSSCAPEFGVPSVVDRFQQIEPKLLLAVEGYRYNGKVHDRR
                     AALAEIRGRLPTLRATVLVPSPGGGPPGAGGHAGNGEVVAWADLLAGAPGGPGALSFE
                     PVPFEHPLWVLYSSGTTGLPKPIVQGHGGIVLEHLKSLSLHLDLTAADRFFWFTTTGW
                     MMWNFLVGGLLLGATVLLWDGSPAHPDLLALWRFAEATGMTYFGTSAAYVLGCAKAGV
                     EPGRDADLSRVHSIGSTGSPLPPEGFAWLYQHVGGDVLVGSVSGGTDMCTAFVQSCPV
                     LPVYAG"
     gene            1518..2435
                     /locus_tag="VEP73_12050"
     CDS             1518..2435
                     /locus_tag="VEP73_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27204.1"
                     /translation="MSTGDLTETRRDRAALARDAGFSKVSFISVLAGVLVAYGAFAVL
                     LAAAAAIAAAAGISNTLTVNNWAKIGVGSAITIAVILLLSYCFGGYVAGRMARRAGLL
                     NGLAVFVLAVLIIAVLAAIAATQVDSSAIVTNLRSLGIPTSGTEWGDTGSVAGLGSLV
                     AMLVGAVLGGILGERWHTKLTRRAATTDTAPAATTPSAASPAATAPSAAAPAAADRDA
                     TPGWGTDRADRDTDRARADRARDPDQAGAGTGRLSEAGTGRPEPVRSERVRASSLFGG
                     EARPGADRTDAAGRGGGRHLGGGGGDTSP"
BASE COUNT          369 a         1123 c         1002 g          316 t
ORIGIN      
        1 ccccgcgtac acgggcagca ccgggcagga ctggacgaac gcggtgcaca tgtcggtccc
       61 gccgctgacc gagccgacca gcacgtcgcc gcccacgtgc tggtagagcc aggcgaaccc
      121 ctcggggggg agcggcgagc cggtcgagcc gatgctgtgg acccgcgaga ggtcggcgtc
      181 gcgccccggc tcgacgcccg ccttggcgca gcccagcacg tacgcggcgc tggtgccgaa
      241 gtacgtcatc ccggtcgcct cggcgaaccg ccacagggcg agcaggtcgg ggtgggcggg
      301 gctgccgtcc cacagcagca ccgtcgcgcc gagcagcagc ccgccgacga ggaagttcca
      361 catcatccag ccggtggtgg tgaaccagaa gaaccggtcc gcggcggtga ggtcgaggtg
      421 caggctgagc gacttcaggt gctcgagcac gatcccgccg tggccctgca cgatcggctt
      481 gggcagcccg gtggtgcccg aggagtacag cacccagagc gggtgctcga acggcaccgg
      541 ctcgaacgac agggcgcccg ggccgcccgg ggcaccggcg agcaggtccg cccaggcgac
      601 cacctccccg ttcccggcgt ggccgccggc gccgggtggc ccaccgccgg gcgaggggac
      661 cagcacggtc gcccgcaggg tcggcaggcg gccgcggatc tccgcgagcg ccgcgcgccg
      721 gtcgtggacc ttgccgttgt agcggtagcc ctccacggcc aggagcagct tcggctcgat
      781 ctgctggaac cggtcgacca cgctgggcac gccgaactcg ggggcgcagc tcgaccagat
      841 cgcgccgagg ctggcgctgg ccaggaacgc caccaccgtc tcgggcacgt tcggcaggta
      901 ggcgaccacc cggtcgcccc ggccgacccc gagccgcctg agcccggccg ccgcagccgc
      961 gacctgccgg tgcagctcgg cgtaggtgac cgtgccgagc gtgccgtcct cggacccgaa
     1021 cacgagcgcc gggtggtcgt cacgccggcg cagcgcgtgc tcggcgtagc tcagctccgc
     1081 gccagggaac cagcgggcgc cgggcatgcg gcgctctgcc agcgccggcg agggcgcgcg
     1141 cccgtccccg accccgaaga actcccagat cgaggtccag aaggcgtcca gctcggtcac
     1201 cgaccagcgc cacaggtcgt cgtaggcgtg gaagtcgagc ccgcggtgct cgctcagcca
     1261 gtgctcgtac cgcacgaggt tggaccgcag gacccgctcc ctcgacggcc tccagagcgg
     1321 ggccggccgg ccggccgcaa ccgcttgcgg gtcgctcgtg accgccacct ccctcgacgt
     1381 ccggcggtgt cgcacgctcc cccaggacgc taccacgcac cctcgagccc ccggccggcc
     1441 agccggccgc cgtttcccgc gcacgttcgt gggaaatgat gcacttcaga gtcgtccgat
     1501 cggaggagaa caccatcatg tccacagggg acctcaccga gaccaggcgg gaccgcgcgg
     1561 cgctggcccg ggacgccggg ttcagcaagg tctcgttcat cagcgtgctg gccggcgtgc
     1621 tcgtcgccta cggcgccttc gccgtgctgc tcgccgcggc cgcggcgatc gcggcggcag
     1681 ctggcatcag caacaccctg accgtcaaca actgggcgaa gatcggggtc ggcagcgcca
     1741 tcacgatcgc cgtcatcctg ctgctctcgt actgcttcgg cgggtacgtg gccggccgca
     1801 tggccaggcg cgccggcctg ctgaacggcc tcgcggtgtt cgtcctcgcc gttctgatca
     1861 tcgccgtcct cgccgccatc gcggcgaccc aggtggactc gagcgcgatc gtgaccaacc
     1921 tgcgcagcct cggcatcccg acctccggta ccgagtgggg cgacaccggg agcgtcgccg
     1981 gcctcgggtc gctcgtggcc atgctcgtcg gcgccgtcct cggcggcatc ctcggcgagc
     2041 gctggcacac caagctcacc cgccgggcgg cgaccaccga caccgccccc gcggccacca
     2101 ccccgtcggc cgcctctccc gcggccaccg ccccgtcggc cgccgctccc gcggctgctg
     2161 accgcgacgc caccccgggc tggggcacgg accgggccga ccgcgacacg gaccgggcgc
     2221 gcgccgaccg cgcccgcgac ccggaccagg ctggggcagg caccggccgc ctctccgagg
     2281 cgggcaccgg ccgccccgag cccgtgcgca gcgagcgcgt ccgcgcgagc agcctgttcg
     2341 gcggcgaggc gcgccccggc gccgaccgga ccgacgccgc cggccgtggt ggcggccggc
     2401 acctgggcgg cggcggcggc gacacgagtc cctgaggccc cgcccctcct cccgtccaag
     2461 ggccgcggcc ggggaggccg cggcccctcg tctggggtcc gcggcacgcc gcggcgcgtg
     2521 gccggagggg cgtgcgagat gagcgcccca tgacccgaca gcggccgtac aatcgtcccg
     2581 aggaggccta aatgcagacg gagggggtca tgccggacag ccagcctcag ccgcccgata
     2641 gcccaccctc cgcggacgcg cctccggacg cgccgccacc tcccgacctg cccgcgtctc
     2701 cggagggcct gccccctccc gacctgccgg cgccgccgga ggttccgttg ccttccgagg
     2761 tcccgccccc cgggcccgtg gcgccgcccc cggatctgcc gccgccgccc
//
LOCUS       DASPYY010000773         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2519220, whole genome shotgun sequence.
ACCESSION   DASPYY010000773 DASPYY010000000
VERSION     DASPYY010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519220"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VEP73_12055"
     CDS             <1..402
                     /locus_tag="VEP73_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT27205.1"
                     /translation="SMALYDRTCRIAVGYQDAEHERQIVQLAAPRAKPALIAQAVELT
                     RATREHPDLRSGASVRGAIDYARLVPELAEVRAVPADDWQAGLDAALTTLSGRVRRHE
                     SCQRSADQVIEEIYRRVRAAGQEGGEDRPGE"
     gene            399..1361
                     /locus_tag="VEP73_12060"
     CDS             399..1361
                     /locus_tag="VEP73_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HYT27206.1"
                     /translation="MTSPPGAPGPGGRQQQRPKPKSGRPDGGTTGRRELARHARFAEI
                     SPEVGVLDEQAMSQALADDPAAFELLAAMTTATDENLRAAAIRLSASIVLERARAGRP
                     AVRGISRLRPVRGATDGDLDIDASIEGVSVARSEARPVAVEDLTTVQWAKAPTAFAVV
                     VDRSGSMSGPRLAAAATVAAACALRAPKEHAVLSFAGTVEVIRPLVSDMTPAVVAGQL
                     LRMRGHGVTRLAAALKAAGEQLAPARARRRVVILLSDCRSTDEDDTLSSARTLPELII
                     LAPAGDHEQAAHLAGLAGARWAAIEHPLDAVEALDHLLETRITA"
     gene            complement(1363..1932)
                     /locus_tag="VEP73_12065"
     CDS             complement(1363..1932)
                     /locus_tag="VEP73_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYT27207.1"
                     /translation="MNPPIRLYMTMSLDGFIAGPDDRTGQELGRDGGRLFNWMDDRMS
                     DGPNGQVFREAMATGAVISGRRTFELAGRWQGDHHDGVPIFVLTHRVEEGDVPPGSAR
                     FVTDVEACARQARAAAGDRAVMVHGAGAAQALLRAGQLDEMEIHLVPVLLGGGRRLFD
                     SLGPDHIELELVRRVEGRDVTHLRYRVSR"
BASE COUNT          286 a          780 c          758 g          262 t
ORIGIN      
        1 tcgatggcgc tgtacgaccg gacctgccgg atcgccgtcg gctaccagga cgccgagcac
       61 gaacgacaga tcgtgcagct cgcggcaccg cgcgccaagc ccgccctgat cgcccaggcg
      121 gtcgagctga cccgggccac ccgtgagcat cccgacctgc gctccggagc ctcggtgcgg
      181 ggcgccatcg actacgcccg tctggttcct gaactcgccg aggtccgcgc cgtcccggcc
      241 gatgactggc aggccggcct ggacgccgcc ctgaccaccc tgtccgggcg ggtccggcgg
      301 cacgagtcct gccagcggtc ggccgatcag gtcatcgagg agatttaccg gcgtgtccgg
      361 gcggccggcc aggagggagg cgaggaccgc ccgggggaat gacgagcccg ccgggcgctc
      421 ccgggcccgg cgggcgccag caacagcggc ctaagccgaa atcggggcgg cccgacgggg
      481 gaaccaccgg tcggcgcgag ctcgcccggc acgcccgctt cgccgagatc agccccgagg
      541 tgggcgtcct ggacgagcag gcgatgagcc aggccctggc cgacgacccg gccgcgttcg
      601 agctgctggc cgcgatgacc accgcgaccg acgagaacct gcgcgccgcc gcgatccggc
      661 tcagcgccag catcgtgctg gaacgagccc gggccgggcg gcccgcggta cgcgggatct
      721 cccggctacg cccggtccga ggggcgaccg acggcgatct ggatatcgac gcctcgatcg
      781 agggcgtctc cgtcgcccgc agcgaggcca ggccggtggc ggtcgaggac ctgaccacgg
      841 tgcagtgggc gaaggcgccc acggcgttcg cggtcgtggt ggaccgctcg ggttcgatga
      901 gcgggccgcg gctggccgcg gccgcgacgg tcgccgccgc ctgcgcgctg cgcgccccga
      961 aggagcacgc cgtgctgtct ttcgccggca cggtcgaggt aatccgcccc ctggtcagcg
     1021 acatgacgcc cgcggtggta gccgggcagc tgctccggat gcgggggcac ggcgtgacca
     1081 ggctggccgc cgcgctcaag gcggcggggg aacagctggc gccggcccgg gctcgccgcc
     1141 gggtggtcat cctgctgtcc gactgccgca gcactgacga ggacgacacg ctgtcctccg
     1201 cccgcaccct gccggagctg atcatcctgg ccccggctgg cgaccacgag caggccgcgc
     1261 acctggccgg cctggccggc gcccggtggg cggcgatcga gcacccgctg gacgccgtgg
     1321 aggccctgga ccacctgctg gagacccgga tcacggcctg agtcagcggc tcacccggta
     1381 tcggaggtgc gtcacgtccc gtccctccac ccgccgtacc agctcgagct cgatgtggtc
     1441 gggaccgagc gagtcgaaca gccgccggcc tccgccgagc aggaccggca cgaggtggat
     1501 ctccatctcg tcgagttgcc ccgcccgcag cagcgcctgc gccgcaccgg ccccgtgtac
     1561 catcacggca cgatcgcccg ccgccgcccg ggcctgccgg gcgcaggcct cgacgtcggt
     1621 gacgaaccgc gcgctgcccg gcgggacgtc cccctcctcg acccggtggg tcaggacgaa
     1681 gatcggcacg ccatcgtggt gatccccctg ccagcgcccg gccagttcga aggtgcgccg
     1741 accggagatc accgcgccgg tcgccatggc ctcgcggaac acctgcccgt tcggcccgtc
     1801 ggacatccgg tcgtccatcc agttgaatag gcgcccgccg tcgcggccga gttcctggcc
     1861 ggtacggtcg tccggcccgg cgatgaagcc gtcgagggac atggtcatgt acaggcggat
     1921 cggcgggttc atgcgtaggc tctcccttcc atgcgatcgt tgtcgtccat gcagacgtgc
     1981 cggaccggaa aactaaccga catgagcgtc gcggcggtca acagcgcgct ccagcgtgcc
     2041 cgcgccacgc tgcgcgatca gtggccggat gggcggctgg gatggg
//
LOCUS       DASPYY010000774         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_271246, whole genome shotgun sequence.
ACCESSION   DASPYY010000774 DASPYY010000000
VERSION     DASPYY010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271246"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VEP73_12070"
     CDS             <1..891
                     /locus_tag="VEP73_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27208.1"
                     /translation="GLVVVAVVAGAFAAGRATQATPAAVQAAPSTTEPDTGQPDQGLA
                     TRGDSFYNWPSRQRAGLTLTLERIEVSERRTTITVGVGGLGQGQSIAGLHGITVTDRA
                     GRQLLGTGPAEPADTERDAGGDQHVARVTVPGGIGDLAAVAAMTVQGVTVASRLDELA
                     KGTLVDPHLSASDVRPPPATCGGCRLDVRCTRCRTMRVVAFAYRHADVVVVLAPKGPR
                     PASVLGGGSPQVLVSDDLTSSDVQPAVSAAPDGTTAVRFEARALDVGGGARRAHGFSI
                     DVQDNLAREVQGPWQMSTQP"
     gene            914..>1768
                     /locus_tag="VEP73_12075"
     CDS             914..>1768
                     /locus_tag="VEP73_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27209.1"
                     /translation="MNGWCPRCDALRGRAAVCPVCHTPLAGLEPERPAAPALPAGAAP
                     AGAAAGPPRIRVALAVAAVVVAGIAFVAGRAGGAGVRPRGGPAATVPATTAGPQPADQ
                     RRALGWSSASRGGVRVTAVAIDRGQEPGEDADAGRLHLRVDGLEPGERVLAFKDLRLR
                     DTGRGLFASPDRSVVGETSGAEALPEAPHGSAVPAQDDQRTYAVSLGPTPPPSQLATV
                     QVGGLVVGRTDGATIGLDTPTPWPDQGPGRAAGLGTDDVTVSPWPREAGIAPLRLEVT
                     AAFVGAGRA"
BASE COUNT          191 a          697 c          702 g          178 t
ORIGIN      
        1 gggctggtgg tcgtcgccgt ggtggccggc gcgttcgcgg ccggccgggc cacccaggcg
       61 actccggccg ccgtccaggc ggcgccgagc accaccgagc ccgacacggg ccagcccgac
      121 cagggcctcg ccacccgcgg cgacagcttc tacaactggc cgagccggca gcgggcgggc
      181 ctgaccctga ccctggagcg gatcgaggtc tcggaacgca ggaccacgat cacggtcggt
      241 gtcggcgggc tcggccaggg ccagtccatc gccggcctgc acggcatcac ggtcaccgac
      301 cgggcaggca ggcagctcct cggcaccggg ccggccgagc ccgccgacac cgagcgcgac
      361 gcgggcggtg accagcacgt ggcgagggtc accgtaccgg gcgggatcgg cgacctcgcg
      421 gccgtcgcgg ccatgacggt ccagggcgtc accgtcgcca gccggctcga cgagctggcc
      481 aaggggacgc tcgtggaccc gcacctgtcc gcctccgacg tgcgcccgcc gccggcgacc
      541 tgcggcggct gccgcctgga cgtgcgctgc acccggtgcc ggacgatgcg ggtggtcgcc
      601 ttcgcctacc gtcacgccga cgtcgtggtc gtgctcgccc ccaagggccc gcggccggcg
      661 tcggtcctcg gtggcgggtc gccgcaggtc ctggtctcag acgacctcac cagctcggac
      721 gtgcagccgg cggtcagcgc cgcgcccgac ggcaccaccg ccgtccggtt cgaggcgcgg
      781 gccctggacg tcggcggcgg cgcgcgccgg gcccacggct tctcgatcga cgtccaggac
      841 aacctcgcgc gggaggtgca ggggccgtgg cagatgagca cccagccgtg aacggacgca
      901 tgggcggccg gccgtgaacg gctggtgccc ccgctgcgac gccctccggg ggcgggcggc
      961 cgtctgcccg gtctgccaca ccccgctggc gggactcgag cccgagcgcc cggcggcgcc
     1021 cgcccttccg gccggcgccg caccggccgg cgcggccgcc ggcccgcccc gcatccgggt
     1081 cgcgctggcc gtggccgccg tggtcgtcgc ggggatcgcg ttcgtcgccg ggcgggcggg
     1141 cggggcgggc gtgcgaccgc ggggcgggcc ggcggccacc gtcccggcca ccacggccgg
     1201 cccgcagccg gccgaccagc gacgcgcgct cgggtggtcg tcggcctcgc gcggcggggt
     1261 cagggtgacc gccgtggcga tcgaccgggg acaggagccg ggcgaggacg ccgacgccgg
     1321 ccggctccac ctgcgcgtcg acgggctcga gcccggcgag cgggtcctgg ccttcaagga
     1381 cctgcggctg cgcgacaccg gccgcgggtt gttcgccagc cccgaccgga gcgtcgtcgg
     1441 cgagacgagc ggggcggagg ccctgcccga ggcgccgcac ggcagcgcgg tcccggccca
     1501 ggacgaccag cggacctacg cggtgtcgct cgggccgacc ccgccaccct cccagctcgc
     1561 caccgtccag gtcggcggcc tggtcgtcgg caggaccgac ggggccacga tcgggctcga
     1621 cacgccgacg ccgtggccgg accaggggcc cggccgagcg gccggcctcg gcacggacga
     1681 cgtgacggtc tcgccctggc cgcgcgaggc cgggatcgcg ccgctgcggc tcgaggtgac
     1741 ggccgcgttc gtgggcgccg ggcgggcc
//
LOCUS       DASPYY010000775         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2237582, whole genome shotgun sequence.
ACCESSION   DASPYY010000775 DASPYY010000000
VERSION     DASPYY010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237582"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VEP73_12080"
     CDS             complement(<1..424)
                     /locus_tag="VEP73_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYT27210.1"
                     /translation="MTGLGAGVVAEQLFAPVPGGVGRYVRALVRHLPAVAEGDGGRVR
                     WIVGRHPPERLAADGLDPAATAALRLPGRVATRAWVDLRRPALPAGLLGSVDVVHATS
                     AAIPPAGGRPLVATVHDLGFRHHPEAYPPAGRRFHERAA"
     gene            complement(421..1581)
                     /locus_tag="VEP73_12085"
     CDS             complement(421..1581)
                     /locus_tag="VEP73_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYT27211.1"
                     /translation="MRVGIDATPLLGPRTGVGRYVAGLVEGFGELAGQAPGGAPGPDE
                     LVLVPFTWRGTRDLPAAVPETSPAGRPLVRCGRRRVPARLLAAAWARLPWPPVEWLAG
                     RVDVFHATNFVSPPARRAAPVMTVHDLTYLRYPAMVTAASARYAELVPRGLGRGAVVC
                     TPTAAVAAEVAEAYGVPGDRVVVTPLGVAPAWHAAVPPSAAWLAARGLPPRYLLFVGS
                     REPRKGLDVLLAAYRELLAGDRDVPPLVLAGPPGWGAALDLAGLPEGAVRLPGYLPED
                     ELASLTAGAAALVFPSRYEGFGLPPLQALACGTAVVASDLPALAEVLGAEADLVPAGD
                     AGALAGALAGVLKDDGGVAARAARRARAAAFTWPACARATLAAYARAREGER"
     gene            complement(1578..2762)
                     /locus_tag="VEP73_12090"
     CDS             complement(1578..2762)
                     /locus_tag="VEP73_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT27212.1"
                     /translation="MAVVSDATIAARGVSKKFQLHHKRATSLKERMLLGRKTVSEDFW
                     ALRDIDLEIGGGETVGLIGPNGSGKSTLLKVLAGILAPTTGTVQVRGRIASLLELGAG
                     FNGELTGRENVYLNASILGLSRREIDRNFDAIVDFSELGPFIDNQVKHYSSGMYVRLG
                     FAVAVHVDPDVLLVDEVLAVGDEAFAAKCLRRVAEFQEQGKTILFVTHGLDLISEICN
                     RAVVLNHGRMLYEGDPAEAVERLRAFMGTAENQEDGLVGSGLLEIADTRVLDPVTGQP
                     SRDFRVGDELVLEVDVKGSPEAPPAGVRLMVTGWADYWMYLLETPPDLLPPGGAPRTV
                     RFRVPSLPNMQRVFSFSVALLKAGTKDVLDARRFGDQFQVHGPAEVGLSKVDYEYRLL
                     PE"
     gene            complement(2762..3634)
                     /locus_tag="VEP73_12095"
     CDS             complement(2762..3634)
                     /locus_tag="VEP73_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT27213.1"
                     /translation="MQSATQSAADARASAQSRIRLELLANLVRKDLKVKYKNSTLGFV
                     WSMANPLLYLAVFTLVFQFFLKSGVPKFAVYLMSGLLVWNFFNTATMGATGSVVGSAN
                     LVKKVRFPRVVLPLASVGFAGVHFALQMLVFFLFLIPFYPHAYGPQLWLLLPAVAVAV
                     LFTVGMSLLVSGLNVRYRDVEHLLEIAMLAWFWLTPIVYSVGLVRKHLEARGLFWLYV
                     ANPMTSVVSAMQRAIYRDPVKQIGGVPTQVLAAGGYGFYLKWLGIAALVSAALIAVGL
                     WTFQRLQADFAEEL"
BASE COUNT          573 a         1457 c         1521 g          452 t
ORIGIN      
        1 gggcggcccg ctcgtggaag cggcggccgg cgggcgggta cgcctcggga tggtggcgga
       61 agccgaggtc gtggacggtc gcgacgagcg gccggccgcc ggcgggcggg atcgccgcgc
      121 tggtcgcgtg gacgacgtcg accgacccga gcagcccggc gggcagggcc ggccgccgca
      181 gatcgaccca cgcccgggtc gcgacccggc cgggcaggcg cagcgcggcg gttgccgcgg
      241 ggtcgaggcc gtcggccgcc agccgctcgg gcgggtgccg gccgacgatc caccgcaccc
      301 gcccgccgtc gccctcggcc accgcgggca ggtgcctgac gagcgcccgc acgtaccggc
      361 cgacgccgcc ggggacgggc gcgaagagct gctcggcgac gacccccgcg ccgaggcccg
      421 tcaccgttct ccctcccgcg ccctggcgta ggcggcgagg gttgcccggg cgcaggccgg
      481 ccaggtgaac gcggccgccc gggcgcggcg ggcggcgcgg gccgccacgc cgccgtcgtc
      541 cttcagcacg ccggcgaggg cgccggcgag ggcgcccgcg tcgcccgccg gcacgaggtc
      601 ggcctcggcg ccgagcacct cggcgagggc gggcaggtcg gaggcgacga ccgccgtgcc
      661 gcaggcaagg gcctgcaggg ggggcagccc gaacccttcg tagcgggacg ggaagacgag
      721 cgcggcggcg cccgcggtga ggctcgccag ctcgtcctcc gggaggtagc cgggcaggcg
      781 caccgcccct tcgggcagcc cggccaggtc gagcgccgcc ccccagccgg gcgggccggc
      841 gagcaccagc ggcgggacgt cccggtcgcc cgcgagcagc tcgcggtagg cggcgagcag
      901 cacgtcgagg cccttgcggg gctcccggct gccgacgaac agcaggtagc gcggtggcag
      961 cccgcgtgcg gcgagccagg cggcgctcgg ggggacggcc gcgtgccatg cgggcgcgac
     1021 gccgaggggc gtcaccacga cccggtcgcc gggcacgcca taggcctcgg cgacctcggc
     1081 ggcgaccgcc gcggtcgggg tgcacacgac cgcgccccgg ccgaggccgc gcgggacgag
     1141 ctcggcgtag cgcgccgagg ccgccgtcac catggccggg tagcgcaggt aggtgaggtc
     1201 gtggacggtc atgacgggag cggcccggcg ggcgggcggg gagacgaagt tcgtcgcgtg
     1261 gaagacgtcc acccggccgg cgagccactc gaccggcggc cagggcaggc gcgcccacgc
     1321 cgccgcgagc agccgggccg gcacgcggcg gcgaccgcag cggaccagtg gccggccggc
     1381 gggggaggtc tccgggaccg cggcgggcag gtcgcgggtg ccccgccagg tgaacggcac
     1441 cagcacgagc tcgtccggcc cgggtgcgcc gccgggcgcc tgcccggcca gctcgccgaa
     1501 gccctcgacg aggccggcga cgtaccgccc gaccccggta cgcggcccga gcagcggcgt
     1561 cgcgtcgatc ccgaccctca ttccggcagg agccggtact cgtagtccac cttcgagagc
     1621 ccgacctcgg ccggcccgtg gacctggaac tggtcgccga accggcgggc gtcgagcacg
     1681 tccttggtgc ccgccttgag gagcgccacc gagaacgaga acacgcgctg catgttcggc
     1741 agggagggga cgcggaagcg cacggtccgc ggcgcgcccc cgggcgggag caggtcgggc
     1801 ggggtctcga ggaggtacat ccagtagtcg gcccagccgg tcaccatcag ccggacgccg
     1861 gcagggggcg cctcgggcga ccccttcacg tcgacctcga gcaccagctc gtcgccgacc
     1921 ctgaagtcgc gggagggctg cccggtcacc gggtcgagca cgcgcgtgtc ggcgatctcg
     1981 agcaggccgc tgccgacgag cccgtcctcc tggttctcgg ccgtgcccat gaacgcccgc
     2041 agccgctcga ccgcctcggc cgggtccccc tcgtagagca tgcgcccgtg gttcagcacc
     2101 acggcgcggt tgcagatctc cgagatcagg tcgagcccgt gggtgacgaa caggatggtc
     2161 ttgccctgct cctggaactc ggcgacgcgc cgcaggcact tggccgcgaa cgcctcgtcg
     2221 ccaacggcga ggacctcgtc gacgagcagc acgtcggggt cgacgtggac ggcgacggcg
     2281 aagccgagcc gcacgtacat gcccgacgag tagtgcttca cctggttgtc gatgaacggc
     2341 ccgagctcgg agaagtcgac gatcgcgtcg aagttgcggt cgatctcgcg ccggctgagc
     2401 ccgaggatcg aggcgttcag gtagacgttc tcccgcccgg tcagctcgcc gttgaagccg
     2461 gcgccgagct cgagcaggga cgcgatccgc ccccggacct ggaccgtgcc ggtggtcggg
     2521 gcgaggatgc cggccagcac cttgagcagg gtcgacttcc ccgacccgtt cgggccgatg
     2581 agcccgaccg tctctccccc gccgatctcg aggtcgatgt cgcgcagggc ccagaagtcc
     2641 tcagagaccg tcttgcggcc gagcagcatg cgctccttga ggctggtcgc ccgcttgtgg
     2701 tggagctgga acttcttgga gaccccccgg gcggcgatcg tcgcgtcgct cactacagcc
     2761 actacagctc ctccgcgaag tcggcctgca ggcgctggaa ggtccacagc ccgaccgcga
     2821 tcagagcggc cgacacgagc gcggcgatcc ccagccactt gaggtagaac ccgtagccgc
     2881 cggcggccag cacctgggtg ggcacgccgc cgatctgctt caccgggtcg cggtagatcg
     2941 cccgctgcat ggcgctgacg acgctcgtca tcgggttggc gacgtagagc cagaacagcc
     3001 ccctggcctc gaggtgcttg cggaccagcc cgaccgagta gacgatcggc gtcagccaga
     3061 accaggccag catggcgatc tccagcaggt gctcgacgtc gcggtagcgg acgttcaggc
     3121 cggacacgag caggctcatc ccgacggtga acagcaccgc gacggcgacc gcgggaagca
     3181 gcagccagag ctgcggaccg taggcgtgcg ggtagaaggg gatgaggaac aggaagaaga
     3241 cgagcatctg cagggcgaag tgcacgccgg cgaagcccac cgaggcgagc ggcaggacga
     3301 ccctgggaaa gcgcaccttc ttgacgaggt tggcgctgcc caccaccgag cccgtggcgc
     3361 ccatggtggc ggtgttgaag aagttccaga cgagcagccc ggacatgagg tacacggcga
     3421 acttcggcac gccgctcttc aggaagaact ggaacaccaa cgtgaacacc gcgaggtaga
     3481 gcagcgggtt ggccatcgac cacacgaacc cgagggtgga gttcttgtac ttgaccttca
     3541 ggtccttgcg gacgaggttg gccagcagct ccaggcggat ccgcgactgc gccgaggcgc
     3601 gggcatcggc cgcgctctgc gtcgcgctct gcatggtgct ctcctgactg cgccggcccg
     3661 cgcgggccgg cgtcgacgtt tcgacgctcg cgtcccaggc ggcggcccgg gacccgcgaa
     3721 gtatagttgc ccctccgaca acgggtcgcc gcgcccgggg gcccggtgcc aggatagtgc
     3781 ctccaccgct gccatggcgg ctcgcgaccg cagcggcatc gtgagcaagt acactgcggc
     3841 gtcatgcgca cccgtcccac ctcgccgacc ccgccgaccg tcctcgagcc cggccgtgcg
     3901 cagcacgccg gcgcgggtgg tccggcggcc gcgccggccc ggatcgagct ccgcccggcc
     3961 gaggccctgt ggctctccgg cgccgcggcc gtcgccgcgg tct
//
LOCUS       DASPYY010000776         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2097233, whole genome shotgun sequence.
ACCESSION   DASPYY010000776 DASPYY010000000
VERSION     DASPYY010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097233"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..>2128)
                     /locus_tag="VEP73_12100"
     CDS             complement(72..>2128)
                     /locus_tag="VEP73_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYT27214.1"
                     /translation="GSTCAATPGSTDYSGVRSHGTWVASLVAGLDDTTGITGVAPAAK
                     VRAWKVDDGAGGIPTSSLVAALTEIVANPAFADVKVVNLSLGGPESSSAEQNAVEALL
                     HDGRSVVASSGNDGNYLPQYPASYPGVLAVGATDAAGNVTTFSSYGKVDVVAPGQGVT
                     VDDPSGSTRLVDGTSFASPIVAGVIALGPAAGSQAALRNRLGVEGTASTSTSHLAATD
                     PKRSGHGLADAAAWIATQDSGAPAYLVAETSGPVADPVTAASSGQLANPGTTFDAYAL
                     KSDGSLGTNPSPASFDVDGSSAASGRSFTDLGGGVFHAASGPLPLSRGATHLGTATAT
                     VGGGSASDTAPVRALRADDQAPGVALSGTGDDAWNRHGVLDGGGDGQVADDNVDDVYA
                     VYLAAGDTLDATLRTQSGPGLAASLYVPGTTDVLSQFDRIVACAASPPDPFCPSTLHL
                     RAPVAGTYLIDVYALGSEFGPTAGGYRLVWTVKNTAGVPVSVQVGACSPNGDGVQDRC
                     AWSVGALSGYAAGSFVTSGFTSVLTVAGTGAKSWDGTARPDGAYTLRVLYSLGPGRRA
                     LLRTFPLTLDRVRPKVANLVVAPNPFEPMPRDGDRDTTTFAITSSERSRLRVLVYKYG
                     TSTLLRTLSTGFQPAGRQRVSWNGRTLAGVQLRGRFAFRMQIIDPAGNAYVTGRYLVV
                     IL"
BASE COUNT          277 a          763 c          820 g          268 t
ORIGIN      
        1 accccggcca cgccggccac ggagccctcg gccggcgagc cctgacccgc ctcgtcccgg
       61 gcggctcggc cttacaggat gacgaccagg tagcggccgg tcacgtaggc gttgccggcc
      121 gggtcgatga tctgcatccg gaacgcgaac cggccgcgga gctgcacacc ggccagggtc
      181 cggccgttcc aggagacgcg ctggcgtccg gcgggttgga acccggtcga gagcgtccgc
      241 agcagcgtcg aggtcccgta cttgtagacc agcacgcgca ggcgcgagcg ctcgctggag
      301 gtgatcgcga acgtcgtggt gtcgcggtcg ccgtcgcggg gcatgggctc gaacgggttg
      361 ggcgccacaa cgaggttggc gaccttggga cggacccggt cgagcgtcag cgggaacgtc
      421 cgcagcagcg cccgcctgcc cggcccgagc gagtacagga cgcggagcgt ataggcgccg
      481 tcgggccggg ccgtcccgtc ccacgacttc gcgcctgtcc ccgcgaccgt cagcacgctg
      541 gtgaacccgc tggtcacgaa cgacccggcc gcgtagccgg agagcgcccc gaccgaccag
      601 gcgcaacgat cctggacgcc gtcgccgttc gggctgcagg cgccgacctg caccgagacc
      661 ggcaccccgg cggtgttctt gaccgtccag acgagccggt agccgcccgc ggtgggcccg
      721 aactcggaac cgagggcgta gacgtcgatc aggtaggtgc cggccaccgg cgcccgcagg
      781 tggagggtgg acgggcagaa ggggtcgggc gggctcgccg cgcacgccac gatgcggtcg
      841 aactggctga gcacgtctgt cgtgcccggg acatacaagg acgcggcgag cccggggccg
      901 ctctgggtcc tcagggtggc gtcgagcgtg tccccggccg ccaggtagac cgcgtagacg
      961 tcgtcgacgt tgtcgtcggc cacctggccg tcgccgccgc cgtcgaggac gccgtgccgg
     1021 ttccaggcgt cgtccccggt ccccgagagg gccaccccgg gcgcctggtc gtcggcgcgc
     1081 agcgcccgca ccggcgccgt gtcgctggcc gagccgccgc cgaccgtggc cgtggccgtg
     1141 ccgaggtggg tggcgccgcg cgagagcggg agcgggcccg acgccgcatg gaagacgccc
     1201 ccgccaaggt ccgtgaacga cctgccagag gcggccgagc tgccgtcgac atcgaacgag
     1261 gccggtgacg ggttcgtccc gaggctcccg tcgctcttca gcgcgtaggc gtcgaaggtg
     1321 gtgcccgggt tggcgagctg gccggaggag gcggcggtga cagggtcggc caccggcccg
     1381 gaggtctcgg cgaccaggta cgcgggcgcg cccgagtcct gggtggcgat ccacgcggcg
     1441 gcgtcggcga gcccatgccc ggagcgcttg ggatcggtcg ccgccaggtg gctcgtcgag
     1501 gtcgaggccg tcccctccac gccgagccgg ttgcgcaggg cggcctggct gccggccgcc
     1561 gggccgaggg cgatcacgcc cgccacgatc ggcgaggcga acgacgtgcc gtccaccagc
     1621 ctggtcgacc cgctggggtc gtccacggtc acgccctggc cgggggcgac cacgtcgacc
     1681 ttgccgtagg aggagaacgt ggtcacgttg ccggccgcgt cggtcgctcc cacggccagc
     1741 acgccagggt aggaggccgg gtactggggc aggtagttgc cgtcgttccc ggacgaggcg
     1801 acgaccgacc tgccgtcatg caggagggcc tcgaccgcgt tctgctcggc gctggacgac
     1861 tcggggccgc cgaggctcag gttgacgacc ttgacgtccg cgaaggccgg gttggcgacg
     1921 atctcggtca gcgcggcgac cagcgacgac gtcgggatcc cgcccgctcc gtcgtcgacc
     1981 ttccacgccc tgaccttggc cgccggggcg acgccggtga tcccggtcgt gtcgtcgagg
     2041 ccggcgacga ggctcgccac ccaggtgccg tggctccgca ccccgctgta gtcggtcgag
     2101 cccggcgtgg ccgcgcaggt cgagccgc
//
LOCUS       DASPYY010000777         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1676747, whole genome shotgun sequence.
ACCESSION   DASPYY010000777 DASPYY010000000
VERSION     DASPYY010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676747"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /gene="infB"
                     /locus_tag="VEP73_12105"
     CDS             <1..2112
                     /gene="infB"
                     /locus_tag="VEP73_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYT27215.1"
                     /translation="GQGRPAPGPGGAGRPGGAGRPAPGGRGAPGGAGGGAGRPGLGGR
                     PGGAGAGRGAPAGARGGPGGGPRGGPGGRGGFGGYRGPRPKKRKGGRRREEFEASPAP
                     TLGQSALDVSVEGVLRVPRGVTVQDLADRLGRPAGDLVRHLMQMGEMLTATQSMSDDA
                     VLLLAEDLGARIEIVDPSEADAVEEIEGWEDAQADRDEDLLPRPPVVTVMGHVDHGKT
                     KLLDAIRHTDVVAGEAGGITQHIGAYQVRAGEDGRLVTFIDTPGHEAFTAMRARGAAV
                     TDVAVLVVAADDGVMPQTIEAINHAKAAGVPIVVAVNKIDKPEANPVRVRQQLTEYDL
                     VAEEYGGKTAFVDVSAKTQQGLDALVENVLLVADVELNLKANPNRRAQGTIIEAHLDR
                     GRGPVATVLVRRGTLKVGDAVAAGTAFGRVRAMFDENGKEVGEAGPARPVQILGLSAP
                     PSAGDEVRAVSDERMARSIAQTRESRHRQAEIASTKAPRLEDLFARIKEGELQELPLV
                     VKADMMGSVEAIDDALNKLDVPEVRMSILHKGAGAITENDVSLAATAGAIIIGFSVRP
                     TPAARELATREGVDIRLYSVIYQVVEDIERALRGLLAPEFEERALGTAEVRQLFRVPR
                     VGVVAGSYVTSGEIARGARARLVRDGVVVYQGRVDSLRRFKEDARTVAAGYECGIGLE
                     NFQDVKERDVIEAYEMVEIPR"
     gene            2191..2484
                     /locus_tag="VEP73_12110"
     CDS             2191..2484
                     /locus_tag="VEP73_12110"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYT27216.1"
                     /translation="MFVALLRLDLRLPDATSLKDKRSVVKSLSAGLRRLNCSVAEVDH
                     HELRQRATLAVATVAGESFHARRVLHSAEREAERTFGVELLDAQITLYGPEDG"
     gene            2492..2905
                     /gene="rbfA"
                     /locus_tag="VEP73_12115"
     CDS             2492..2905
                     /gene="rbfA"
                     /locus_tag="VEP73_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYT27217.1"
                     /translation="MANSSYPRSRRLAETVRRLVSEWVEAEHADGRLGLVTVTDVRMT
                     GDLRHATVWWTVLGGEEEQAATAAALDEVVGQARTYVAQRVRIRHAPTLEFRLDDVPG
                     HGARIDRLLAELAVDGGAPSEPAADNAPNQEGSHR"
     gene            2902..3927
                     /locus_tag="VEP73_12120"
     CDS             2902..3927
                     /locus_tag="VEP73_12120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018253623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HYT27218.1"
                     /translation="MTTSREPITEAEWQAAVAAIDGAGQTVISCHLNPDGDALGSALA
                     MQRALERAGRPAVTSFSEPFVVPPQYRFLPGLDRLTPPSRLPADPDLLVCFDAGSLDR
                     LGSLVPAFEAAGRTVVVDHHASNTRFGDVNLVDPAAPASAVLCRELLRRLGLPLDPEI
                     ATALYTGLVTDTGRFQYQATTPATHQLAAELLAAGVRQYEVSKALFETNDIGYLRLAA
                     DALGRVAQVPEASLVWTSVTRADLAARGVEMDQTEGLIDLVRTDAASDVAAVVKEQPD
                     GSHKVSLRSKGGTNVGELATRFGGGGHAFAAGFTSRLGVEGTIWSIVDALAGRDADRA
                     PARPLAG"
     gene            3991..4866
                     /gene="truB"
                     /locus_tag="VEP73_12125"
     CDS             3991..4866
                     /gene="truB"
                     /locus_tag="VEP73_12125"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYT27219.1"
                     /translation="MDGAIVCDKPAGMTSHDVVARVRRLTGQRRVGHGGTLDPPATGV
                     LVVALGRATRLLPYLPMEPKRYRAAVAFGAETDTLDAAGTVVATADATAVDRRAVERA
                     LARFVGPGEQVPPMVSAVKVGGERLYAKARRGETVERAPRPITVYALDLVDFVPGDRP
                     LATVDVACSGGTYVRALAADLGRAVGSLAHLAALRRTAVGRFTEEAAHPLAELETLAG
                     AGELGSVLLDPAAAMAPVEVRALSAAEAAALAAGRTLLPTGLAGPVAAVAPDGRLVAV
                     IQDGSGRARPKVVLG"
     gene            4863..>5414
                     /locus_tag="VEP73_12130"
     CDS             4863..>5414
                     /locus_tag="VEP73_12130"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HYT27220.1"
                     /translation="MTGAAVRVYHGLAELPADLGPTVVTVGMFDGVHRGHQALLRQVA
                     AEAATRGVPAAAVTFDRHPMEVLRPGSHPLLLTTLGQKIDLLGRAGMEVVLVLPFTLE
                     LSHVPAGEFATRVLFGAMGARAVVVGTNFRFGHKAQGDVELLAALGRERGVDAVGVAL
                     HADERHEVVSSTRIRAELARGDVQ"
BASE COUNT          683 a         2079 c         1993 g          659 t
ORIGIN      
        1 ggtcagggcc ggccggctcc cggacccggc ggtgccggtc gaccgggtgg cgcgggccgt
       61 ccggcccccg gcgggcgagg tgctcccggc ggggccggtg gcggggccgg acggcccggc
      121 ctcggaggtc gcccgggtgg cgccggtgcc ggccgcggcg cgcccgcggg cgcgcgcgga
      181 ggtcccggtg gcggtccccg cggcggtccc ggtggccggg gcggcttcgg cgggtaccgc
      241 ggcccgcgcc cgaagaagcg caagggcgga cgccggcgcg aggagttcga ggcctcgccg
      301 gcgccgaccc tcggccagtc cgcgctcgac gtctcggtcg agggcgtcct gcgcgtgccc
      361 cgcggggtca ccgtgcagga cctggccgac cggctcggcc ggccagcggg cgatctggtc
      421 cgccacctca tgcagatggg ggagatgctg accgccaccc agtcgatgtc cgacgacgcc
      481 gtcctgctgc tcgccgagga cctcggtgcc cgcatcgaga tcgtcgaccc gagcgaggcc
      541 gacgcggtgg aggagatcga gggctgggag gacgcccagg ccgaccgcga cgaggacctg
      601 ctcccgcggc cgccggtggt caccgtcatg gggcacgtcg accatggcaa gaccaagctg
      661 ctcgacgcga tccgccacac cgacgtggtc gcgggcgagg ccggcggcat cacccagcac
      721 atcggggcct accaggtccg cgccggtgag gacggccggc tggtcacctt catcgacacc
      781 ccgggccacg aggcgttcac cgccatgcgt gcccgcggcg ccgccgtgac cgacgtcgcc
      841 gtgctcgtcg tggcggccga cgacggcgtc atgccgcaga ccatcgaggc gatcaaccac
      901 gccaaggcgg ccggggtgcc gatcgtcgtc gccgtcaaca agatcgacaa gccggaggcc
      961 aacccggtcc gggtccgcca gcagctcacc gagtacgacc tggtggccga ggagtacggc
     1021 ggcaagacgg ccttcgtgga cgtctcagcc aagacccagc agggtctcga cgccctggtc
     1081 gagaacgtgc tgctcgtcgc cgatgtcgag ctcaacctga aggcgaaccc caaccggcgc
     1141 gcccagggca cgatcatcga ggcgcacctg gaccggggcc gcggcccggt ggcgaccgtg
     1201 ctggtccgcc ggggcaccct caaggtgggg gacgcggtgg cggcgggcac ggcgttcggc
     1261 cgggtccgcg ccatgttcga cgagaacggc aaggaggtcg gcgaggccgg cccggcccgt
     1321 ccggtccaga tcctcggcct ctccgccccg ccgagcgcgg gcgacgaggt ccgggcggtg
     1381 tccgacgagc ggatggcccg ctcgatcgcc cagacccggg agtcgcgcca ccgccaggcg
     1441 gagatcgcct cgacgaaggc gccgcgcctg gaggacctgt tcgcgaggat caaggagggt
     1501 gagctccagg agctgccgct cgtcgtcaag gccgacatga tgggctcggt cgaggccatc
     1561 gacgatgcgc tgaacaagct cgacgtgccc gaggtgcgca tgagcatcct gcacaagggc
     1621 gccggcgcga tcaccgagaa cgacgtgtcg ctggcggcca cggccggggc gatcatcatc
     1681 ggcttctcgg tgcggccgac cccggccgcg cgcgagctgg ccacccgcga gggcgtcgac
     1741 atccgtctct actcggtcat ctaccaggtc gtcgaggaca tcgagagggc cctgcgcggc
     1801 ctgctcgccc cggagttcga ggagcgcgcg ctcgggaccg ccgaggtcag gcagctcttc
     1861 cgggtgccaa gggtgggcgt ggtcgccggg tcctacgtca ccagcggcga gatcgcccgc
     1921 ggggccaggg cccgcctggt ccgcgacggc gtcgtcgtct accagggccg cgtcgactcg
     1981 ctgcgcaggt tcaaggagga cgcgcggacg gtggcggccg ggtacgagtg cggcatcggc
     2041 ctcgagaact tccaggacgt caaggagagg gacgtcatcg aggcctacga gatggtggag
     2101 atcccgcggt aggccgtttg gcccccaccg cggccgtgta cccaaggtgc cgggcctccc
     2161 cggaggcccg gcacccagcc cgggaagaac atgttcgtcg ccctgctccg cctcgacctg
     2221 cgcctgcccg atgcgacgtc gctgaaggac aagcgctcgg tcgtcaagtc gctgtccgcc
     2281 ggcctgcgcc gtctcaactg ctcggtggcc gaggtcgacc atcacgagct gcgccagcgg
     2341 gccaccctcg ccgtggctac ggtggccggc gagagcttcc acgctcgccg ggtgctgcac
     2401 tcggcggagc gggaggccga acgcaccttc ggcgtcgagc tgctcgacgc ccagatcact
     2461 ctctacggac ccgaggacgg ctgacatcga gatggccaac tccagctacc cgcgctcgcg
     2521 gcgcctcgcc gagacggtca ggcgtctggt gagcgagtgg gtcgaggccg agcatgccga
     2581 cggcaggctc ggcctcgtga ccgtgaccga cgtgcgcatg accggcgacc tgcggcacgc
     2641 gaccgtgtgg tggacggtgc tcggcggcga ggaggagcag gcggcgaccg ccgccgcgct
     2701 cgacgaggtg gtggggcagg cgcgcaccta cgtggcccag cgggttcgga tccgccacgc
     2761 cccgacgcta gaattccggc tcgacgacgt gccaggccac ggcgcccgca tcgaccggct
     2821 gctggccgag ctcgccgtcg acggcggcgc gccgtccgag cccgcggccg acaacgcccc
     2881 gaaccaggaa gggtcgcacc gatgacgacc agccgggagc cgatcaccga ggcggagtgg
     2941 caggcggcgg tcgccgccat cgacggcgcc ggccagacgg tgatctcctg ccacctgaac
     3001 ccggacgggg acgccctcgg ctcggccctt gccatgcagc gcgcgctgga gcgcgcgggc
     3061 cggcccgcgg tcaccagctt ctcggagccg ttcgtcgtcc ccccgcagta ccgcttcctc
     3121 cccgggctcg accgcctcac cccgccgtcc cggctgccgg ccgatcccga cctgctcgtc
     3181 tgcttcgacg ccggctccct cgaccggctc ggcagcctcg tccccgcctt cgaggccgcg
     3241 ggccgcaccg tcgtcgtcga ccatcacgcc tccaacaccc ggttcggcga cgtgaacctg
     3301 gtcgacccgg ccgccccggc cagcgccgtg ctctgccggg agctgctgcg ccgcctgggc
     3361 ctcccgctcg accccgagat cgccaccgcg ctctacaccg gcctggtgac cgacaccggg
     3421 cgcttccagt accaggccac caccccggcg acccaccagc tcgccgccga gctgctcgcc
     3481 gccggcgtcc ggcagtacga ggtgtccaag gccctgttcg agaccaacga catcggctac
     3541 ctgcgcctgg cagccgacgc gctcggccgg gtcgcccagg tccccgaggc gtcgctcgtg
     3601 tggacctcgg tgacccgggc cgacctcgcc gcccgcggcg tcgagatgga ccagaccgag
     3661 gggctcatcg acctcgtgcg caccgacgcc gcgagcgacg tggccgccgt cgtcaaggag
     3721 cagcccgacg gctcccacaa ggtctccctc cgctccaagg gcggcaccaa cgtcggcgag
     3781 ctcgccaccc ggttcggcgg cggcggccac gcgttcgcgg ccgggttcac ctcccggctc
     3841 ggggtcgagg ggaccatctg gtccatcgtc gacgccctgg ccggccggga cgccgaccgc
     3901 gcccccgccc gcccgctggc cggataggcg aagcgccgcg tgaccacggc gggcgcaacc
     3961 ccgggccggc gcggcggccc ggcgccgtcc atggacggcg ccatcgtctg cgacaagccg
     4021 gccgggatga cctcccacga cgtggtagcc agggtgcgcc ggctgaccgg ccagcgccgt
     4081 gtcggccatg gcggcaccct cgacccgccg gccacgggcg tgctcgtggt cgcgctcggc
     4141 cgggccaccc gcctcctgcc ctacctgccc atggagccca agcgctaccg cgccgccgtc
     4201 gcgttcggcg cggagaccga cacgctcgac gccgccggga cggtcgtcgc gaccgccgac
     4261 gccaccgccg tcgaccggcg ggccgtcgag cgggcgctcg cccggttcgt cggtcccggc
     4321 gagcaggtcc cgccgatggt ctcggccgtc aaggtgggcg gcgagcggct ctacgccaag
     4381 gcgcgccgcg gggagaccgt cgagcgggcg ccgcgcccga tcaccgtgta cgcgctcgac
     4441 ctggtcgact tcgtccccgg cgaccgaccg cttgcaaccg tcgacgtggc ctgctccgga
     4501 ggtacctacg tgcgggcgct cgccgccgac ctgggccgcg ccgtcggctc cctcgcccac
     4561 ctggccgcac tgcggcggac cgcggtgggc cgcttcaccg aggaggcggc gcatccgctg
     4621 gccgagctcg agacgctcgc cggtgccggg gagctcggtt cggtcctgct cgacccggcg
     4681 gcggccatgg ccccggtcga ggtccgggcg ctgtcggccg ccgaggcggc cgccctcgcc
     4741 gccgggcgga ccctcctccc gaccggcctc gccggcccgg tcgccgccgt cgcgccggac
     4801 ggccggctcg tcgcggtcat ccaggacggc tccggccggg cccgcccgaa ggtggtgctc
     4861 gggtgaccgg ggcggccgtg cgcgtctacc acgggctcgc ggagctgccc gccgacctcg
     4921 gcccgaccgt cgtcaccgtc ggcatgttcg acggggtcca ccgcggccac caggcgctgc
     4981 tgcggcaggt ggccgcggag gcggccacca ggggcgtgcc ggccgcggcg gtcacgttcg
     5041 accgccaccc gatggaggtc ctccggccgg gcagccaccc gctgctcctg accaccctcg
     5101 gccagaagat cgacctgctc ggcagggccg gcatggaggt cgtgctcgtc ctgccgttca
     5161 cccttgagct gtcccacgtg ccggccggcg agttcgcgac gcgggtcctg ttcggcgcca
     5221 tgggcgcccg cgcggtggtg gtgggcacca acttccgctt cggccacaag gcccagggcg
     5281 acgtcgagct gctcgccgcc ctgggccgcg agcgcggcgt cgacgccgtg ggggtcgccc
     5341 tgcacgccga cgagcggcac gaggtcgtgt cgtcgacccg gatccgggcc gagctggccc
     5401 gcggcgacgt ccag
//
LOCUS       DASPYY010000778         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2238246, whole genome shotgun sequence.
ACCESSION   DASPYY010000778 DASPYY010000000
VERSION     DASPYY010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238246"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VEP73_12135"
     CDS             complement(<1..1110)
                     /locus_tag="VEP73_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27221.1"
                     /translation="MEEPVPPTVGTGAGPVRRLRAGWLLRSTGTLALLASGWLWLNLD
                     RPRGPLWTGWLPGLLSGPLAALACRRAATTKGLAAGARRYWNQIGLAIALVTLGTGVQ
                     AYEVLTDPSKPAVTTVLPAALIDLAGVGTGLWALLRLPVGTRSRKDWRMLGLDAGTVM
                     LAATVLVWRFAMSPALEQYDDPALLWGTMAMLVLALIAVFAVVKVVLAGTGPVDRVAL
                     VLLGLADLVGGASGSLVPLFAHQPHLSVMQPLMPVLCLFVAGAAERQRHATLGGGPRG
                     ATTGRPRRPYSLLPYAAVAAIDALLLWTTAPGPGRAGRGRRGGRAHRPGRRAPGRRLP
                     RQRPAARPGGRSGGGARPPGVPRRADPARQPVAVRR"
     gene            complement(1229..>1531)
                     /locus_tag="VEP73_12140"
     CDS             complement(1229..>1531)
                     /locus_tag="VEP73_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYT27222.1"
                     /translation="VTSIEAARSGMTLVDDLQGAGYDQQIGLGVYDIHSPRVPRAADM
                     EALLRRVLAVLDPSQLWVNPDCGLKTRGYDEVTASLRNLVEAARTVRGQLGAPRAG"
BASE COUNT          203 a          598 c          563 g          167 t
ORIGIN      
        1 tcgccgaaca gcgaccggtt ggcgagctgg gtcagctcgt cgtgggacgc ctggtggccg
       61 agcgcctccg ccgctgcgtc cacccgggcg agcagccggg cgttgtcgcg gaaggcggcg
      121 acctggcgca cggcgaccag ggcggtgagc gcgaccgccc cggcgaccac gacccgcccg
      181 tcccggtcca ggtgcggtcg tccagagcag cagcgcgtcg atggcggcca ccgccgcgta
      241 cggcagcagg ctgtacggcc gccgtggcct gccggtcgtg gcgccccgcg gcccgccccc
      301 gagcgtggcg tggcgctgcc gctcggccgc gcccgccacg aacaggcaca gcaccggcat
      361 gagcggctgc atgacgctca ggtgcggctg atgggcgaac agcggcacga gcgacccgct
      421 cgcccctccc accaggtcgg ccagcccgag caggacgagc gccacgcggt ccaccgggcc
      481 ggtgcccgcg agcacgacct tgacgaccgc gaagacggcg atgagcgcca ggacgagcat
      541 ggccatggtg ccccacagca gcgcggggtc gtcgtactgc tcgagcgccg ggctcatcgc
      601 gaagcgccag acgagcacgg tcgcggccag catgaccgtg cctgcgtcca ggccgagcat
      661 ccgccagtcc ttgcgggacc gggtccccac cgggaggcgg agcagcgccc acagcccggt
      721 cccgaccccg gcgaggtcga tcagcgcggc ggggagcacg gtcgtgaccg ccggcttcga
      781 ggggtcggtg agcacctcgt acgcctgcac gccggtgccg agcgtgacga gcgcgatcgc
      841 caggccgatc tggttccagt agcggcgcgc cccggccgcg aggcccttgg tcgtggccgc
      901 cctgcggcag gccagggccg cgagcggccc gctcagcagc ccgggcagcc atccggtcca
      961 cagcggcccc cggggccggt ccaggttgag ccagagccag ccggaggcca gcagcgccag
     1021 cgtcccggtt gaacgcagca gccagcccgc ccgaaggcgc cgcaccgggc ccgccccggt
     1081 gccgaccgtc ggcggcactg gctcctccat gcccccctgc tcctggttgc gcggccaagg
     1141 cacgacacgc tgcgtagtct ccgatcggct cccggcacgc gctcctgagc gattccggcc
     1201 gggcggccgc gcaccgccgc gggcaggcct accccgcccg cggcgcgccg agctgcccgc
     1261 ggaccgtcct ggccgcctcg accaggttgc gcagcgaggc ggtgacctcg tcgtagccgc
     1321 gggtcttcag cccgcagtcc gggttcaccc acagctggct cgggtccagg acggccagca
     1381 cgcgccgcag cagcgcctcc atgtcggccg cccggggcac ccgcggggag tggatgtcgt
     1441 agacaccgag cccgatctgc tggtcgtagc ccgctccctg gaggtcgtcg accagggtca
     1501 tgcccgagcg ggcggcctcg atcgaggtca c
//
LOCUS       DASPYY010000779         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_129625, whole genome shotgun sequence.
ACCESSION   DASPYY010000779 DASPYY010000000
VERSION     DASPYY010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129625"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VEP73_12145"
     CDS             <1..1364
                     /locus_tag="VEP73_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HYT27223.1"
                     /translation="FWIWCAANISILGVTYGTYLVTFYGLNLPQALAAGTAAVILSFL
                     LVGYISLAGKLGGAPTLVLSRAPFGVVGNALPTLVSYISLVGWETILVALATLAADTL
                     LGRLGVHTGTATRAVSFVVIAAATIGIGLLGHATIVKIQTWFTWAFAALTVVFVALEL
                     PQVDWGKVTSLPSGKFLSGFVGGMSVVMAGLGIGWVNAAADYSRYLPRASSSRAVVWW
                     TTFGASVAPVALIAFGVLLAANNADLASSANPIGELAKPLPTWFLVPYLLVAVGGLLA
                     GAVLDIYSSGLNLLTLGVRLERYKSVAIDGVLMIVGCLYILFRAENFVGPFTGFLVTL
                     GVPLAAWAAIFLVDMWLLRRRAGYDERDLYDPDGRYGAVNAAGVGSFLVATVVGLGLV
                     TSTSPAFSWVGYLLRFVGGKHGAFGGSSIGLFVGFVVAAVLYAVLGPVAARGRQPAAA
                     AAR"
     gene            1361..2002
                     /locus_tag="VEP73_12150"
     CDS             1361..2002
                     /locus_tag="VEP73_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HYT27224.1"
                     /translation="MTTAGAEDGAPGGAPDRPRGGDGAPGAADGLLVVVDMQRVFGEP
                     DSPWATPGFDTVVGPIARLVAAFGERVAFTRFLVPDVPEGSWTGYYELWRFAREPGAE
                     RLLELAAPFAGGPHPLVEKPTFSKWGPELRALAGPAGTLVLCGVATDCCVIATAVAAA
                     DAGMYVRVVADACRGIDDAAHERALAVMAGFPPQITLTTVERELSQGPPPAAT"
     gene            2135..2398
                     /locus_tag="VEP73_12155"
     CDS             2135..2398
                     /locus_tag="VEP73_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27225.1"
                     /translation="MDMDEVDRDVRTAERVTDEEIETVWTGGDPGKVVARVDDPRDAD
                     GTDTDGTDGTDGDGTDGTDGTDGDAGDTDGTDGGATDADSKDA"
     gene            2425..3579
                     /locus_tag="VEP73_12160"
     CDS             2425..3579
                     /locus_tag="VEP73_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT27226.1"
                     /translation="MSDPPGALRRCVGDVDRFLGERWARGVHHHRGEDYGDLLSLDHV
                     DRIVATMSLRAPAFRLVRQGRTLERSSYTRTARIGSRAVDDLIDVGRVYRHFGDGATI
                     VLQGLQRYWEPVTRFCRDLELALTHPVQANAYITPPVSTGLKVHDDPHDVFALQTYGR
                     KQWVTYEPGGPAGPTPALDVELEPGDALYLPKGTPHAARTVDVASVHLTIGIRTVTWR
                     DVARRVLGQALDADGLDEPLPAGFAHDPDGLAGLLAARLPALAARVAGADPGAAAGAE
                     ARRFWAARPPVLTGHLRQLLELDRIDDRARLARRGGSVCELAATNGRLAVQLGDRELR
                     MPSALEPAMRVVAGRDSFVVGELAPYLDRESRLVLARRLVREGLLTVAGD"
     gene            3572..>4217
                     /locus_tag="VEP73_12165"
     CDS             3572..>4217
                     /locus_tag="VEP73_12165"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HYT27227.1"
                     /translation="MTERFRCSPTSRARGEPRYGTASTVRRWILVEAPSPWGRDAVLD
                     SRLPPGVGPALKALAQALGARVLLIRRHGRSRPAERTCLVARTTLDTTSVERLPFDDP
                     AELLDRDWTPLVHDEPVGGLPVDRPVYLVCTNGRHDPCCAEYGRRLAVALSTAVGDGL
                     WECSHFGGDRFAGNLVCLPHGVYYGHVEPWHGPAVAAAYAEGRIDLEHYRGRSCH"
BASE COUNT          481 a         1641 c         1516 g          579 t
ORIGIN      
        1 tgttctggat ctggtgcgcc gccaacatca gcatcctcgg cgtcacctac gggacctacc
       61 tggtcacgtt ctacggcctg aacctccccc aggcgctggc ggccggcacc gccgccgtca
      121 tcctgtcgtt cctgctcgtc ggctacatca gcctcgcggg caagctcggc ggcgcaccca
      181 cgctggtgct gtcccgggcg ccgttcgggg tcgtcgggaa cgcgctgccc acgctcgtca
      241 gctacatctc gctcgtcggc tgggagacga tcctcgtcgc gctggccacg ctggccgccg
      301 acacgctcct cggtcgcctc ggggtccaca ccggcaccgc cacgcgggcg gtgtcgttcg
      361 tcgtcatcgc cgccgccacg atcgggatcg gcctgctcgg ccacgccacc atcgtgaaga
      421 tccagacctg gttcacctgg gcgttcgccg ccctcacggt cgtgttcgtg gccctggagc
      481 tgccccaggt cgactggggc aaggtgacct ccctgccgtc gggcaagttc ctctccgggt
      541 tcgtcggcgg gatgtcggtg gtgatggccg gcctcggcat cggctgggtg aacgcggccg
      601 ccgactacag ccgctacctg ccgagggcct cgagctcgcg ggcggtggtc tggtggacca
      661 ccttcggcgc cagcgtcgcg ccggtcgccc tgatcgcgtt cggcgtgctg ctcgcggcca
      721 acaacgccga cctggccagc tcggcgaacc cgatcggcga gctcgccaag ccgctgccga
      781 cctggttcct cgtgccctac ctgctggtcg cggtcggcgg cctgctcgca ggtgccgtgc
      841 tggacatcta ctcctccggt ctcaacctgc tcaccctcgg ggtgcggctc gagcgctaca
      901 agtcggtggc catcgacggg gtgctcatga tcgtcggctg cctctacatc ctgttccgcg
      961 ccgagaactt cgtcggaccg ttcaccggct tcctcgtcac cctcggggtg ccgctggccg
     1021 cctgggcggc gatcttcctg gtcgacatgt ggctgctgcg ccgccgcgcc ggctacgacg
     1081 agcgggacct ctacgacccg gacgggcgct acggcgcggt caacgccgcg ggcgtcgggt
     1141 ccttcctcgt ggcgacggtc gtcgggctcg gcctcgtcac ctccaccagc cccgccttct
     1201 cctgggtcgg gtacctcctg cgcttcgtcg gcggcaagca cggcgcgttc ggcggatcca
     1261 gcatcggcct gttcgtcggg ttcgtggtcg ccgcggtgct gtacgcggtc ctgggcccgg
     1321 tcgccgcccg cggcaggcag ccggcggccg ccgcggcccg gtgaccacgg cgggcgcaga
     1381 ggacggcgcg ccggggggcg cgccggaccg gccgcggggc ggggacgggg cgccgggcgc
     1441 cgccgacggg ctgctcgtcg tcgtcgacat gcagcgggtg ttcggcgagc cggacagccc
     1501 ctgggccacg cccgggttcg atacggtcgt cggccccatc gcccggctcg tcgcggcgtt
     1561 cggggagcgg gtcgcgttca cccgcttcct ggtgcccgac gtgcccgagg ggagctggac
     1621 cggctactac gagctgtggc ggttcgcccg cgagcccggg gccgagcggc tgctggagct
     1681 cgccgcgccg ttcgcgggcg gcccgcaccc gctcgtcgag aagccgacgt tctccaagtg
     1741 gggccccgag ctgcgggcgc tcgccggccc ggcagggacg ctcgtgctgt gcggggtcgc
     1801 caccgactgc tgcgtgatcg cgaccgccgt cgccgccgcc gacgccggca tgtacgtacg
     1861 cgtggtggcc gacgcgtgcc gggggatcga cgacgcggcg cacgagcggg cactggccgt
     1921 gatggccggc ttcccgcccc agataacgct caccacggtc gagcgcgagc tgtcgcaggg
     1981 cccgccgccc gccgccacct gacgccgtcc cggccgcggc gccgggcgaa ccgccacagc
     2041 ccggagcggc cgcgctctcg attgcccggc cggccccgtc gcgctatgtt cgcaccccgg
     2101 tcgggcatga gcatgcggca gcatggaggt ggacatggac atggacgagg tggacaggga
     2161 cgttcggacc gccgagcggg tcaccgacga ggaaatcgag acggtctgga cgggcggcga
     2221 ccccggcaag gtcgtggcgc gggtcgacga ccctcgtgac gccgacggga ccgacaccga
     2281 cggcaccgac ggcaccgacg gcgacggcac cgacgggacc gacgggaccg acggcgacgc
     2341 tggcgacacc gacggcaccg acggcggcgc gaccgacgcc gacagcaagg acgcgtaggc
     2401 gccgcgcccg taccggggac caccgtgtcg gatccccccg gagccctgcg gcgctgcgtc
     2461 ggcgacgtcg accgcttcct cggcgagcgc tgggcccggg gcgtccacca ccaccgcggc
     2521 gaggactacg gcgacctcct ctcgctcgac cacgtcgacc gcatcgtcgc gaccatgtcg
     2581 ctccgcgccc cggcgttccg cctcgtcagg caggggcgga ccctggagcg gtcctcctac
     2641 accaggaccg cccggatcgg ctcccgcgcg gtcgacgacc tgatcgacgt cggccgggtc
     2701 taccggcact tcggcgacgg cgcgacgatc gtcctgcagg gcctgcagcg ctactgggag
     2761 ccggtgacgc gcttctgccg cgacctggag ctcgccctca cccaccccgt gcaggcgaac
     2821 gcctatatca ccccgcccgt gtccaccggg ctcaaggtgc acgacgaccc ccacgacgtc
     2881 ttcgcgctgc agacctacgg ccgcaagcag tgggtgacct acgagcccgg gggaccggca
     2941 gggccgaccc cggcgctcga cgtcgagctc gagccggggg acgccctcta cctgcccaag
     3001 gggacgccac acgccgcccg gaccgtcgac gtcgcctcgg tgcacctcac catcgggatc
     3061 cggacggtga cgtggcggga cgtcgcccgg cgggtgctcg ggcaggcgct cgacgcggac
     3121 gggctcgacg agccgctccc tgccgggttc gcgcacgacc ccgacgggct cgccggcctg
     3181 ctcgccgccc ggctgcccgc gctcgccgcg cgggtcgccg gtgccgaccc gggcgccgcc
     3241 gccggcgcgg aggcccggcg gttctgggcc gcccgcccac ccgtgctcac cgggcacctg
     3301 cggcagctcc tcgagctcga ccgcatcgac gaccgcgccc ggctcgcgcg ccgcggcggc
     3361 tcggtctgcg agctggcggc gaccaatggc cggctcgccg tgcagctagg ggaccgggag
     3421 ctgcgcatgc cctccgcact ggagccggcc atgcgggtcg tcgccggccg cgacagcttc
     3481 gtcgtcggcg agctggcacc ctacctcgac cgggagagca ggctggtgct cgcccgccgg
     3541 ctggtccgcg aggggttgct gacggtcgcc ggtgactgag cggttccgct gctcgccgac
     3601 gtcgcgggcc cgaggcgagc cccgctacgg cacggcgtcg accgtgcggc gctggatcct
     3661 cgtcgaggcg ccgagcccct ggggccggga cgccgtgctc gacagccggc tgccgccggg
     3721 cgtcgggccc gccctgaagg ccctcgcgca ggccctgggc gcccgggtcc tgctcatccg
     3781 caggcacggg cggtcgcgcc cggccgagcg gacctgcctg gtcgcccgca cgacgctcga
     3841 caccacgagc gtcgagcgcc tccccttcga cgacccggcc gagctgctcg accgcgactg
     3901 gacgccgctc gtgcacgacg agccggtcgg gggcctgccg gtcgaccggc ccgtgtacct
     3961 ggtgtgcacc aacggccgcc acgacccctg ctgcgccgag tacggccgcc ggctggccgt
     4021 cgctctctcg accgcggtcg gcgacggcct ctgggagtgc tcccacttcg gcggcgaccg
     4081 cttcgccggg aacctggtct gcctgccgca cggcgtctac tacgggcacg tcgagccgtg
     4141 gcacggcccg gccgtcgccg ccgcctacgc cgagggccgc atcgacctgg agcactaccg
     4201 cggccgctcc tgccacc
//
LOCUS       DASPYY010000780         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2098217, whole genome shotgun sequence.
ACCESSION   DASPYY010000780 DASPYY010000000
VERSION     DASPYY010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098217"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VEP73_12170"
     CDS             complement(<1..600)
                     /locus_tag="VEP73_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HYT27228.1"
                     /translation="MAGYLGPPAPLSTLLSDPPHSLSVQAYQPRLQHDGVVNVDGTGV
                     VWWPVGEREPVRYATERPPWSDPNLLHLAGRLRGVVQLAAVRSGTPGLPYGVDAAAPF
                     AHGTLAVVHNGRVGGFRGPVGRRLLDALPDDLYHAAGTLTDSVVLFLTLVRYHRARPG
                     ASLASTVTAAVLDVAKACADAGEPATLTVLASDGHRIIGV"
     gene            complement(609..>1847)
                     /gene="egtB"
                     /locus_tag="VEP73_12175"
     CDS             complement(609..>1847)
                     /gene="egtB"
                     /locus_tag="VEP73_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HYT27229.1"
                     /translation="APLDERELHRQYDPIMSPLVWDVGHVGNFEELWLLRELDGRAAH
                     DPTLDQVYNPFENPRWVRADLPILPRDEVVAYLADVRGDVLSILRSISFDPDRPLLAA
                     GYVYRMITQHEAQHQETMLQALDLRSDARPYPLAARPRPALRRPVDDAERVVVPGGPF
                     DMGTDDRTAAYDNERPAHRVDVATFAIDRFPVTARRYAAFVAAGGYDHPEHWTEAGWA
                     WRLETGHRAPQGWLSDGSGGWLVRRFDHVLPLDPREPVQHVSAHEALAFARWAGSRLP
                     SEAEWEKAAAWDPLARRSRTYPWGEAPPGPERANLDIAAWGPAPVGSYPAGASAYGVE
                     QLLGDVYEWTCSPFTGYPGYTSFPYPEYSEVFFGDDYLVLRGASWATSRWVARTTFRN
                     WDYPQRRQLFAGLRLAWDVR"
BASE COUNT          249 a          617 c          757 g          224 t
ORIGIN      
        1 gacgccgatg atgcggtggc cgtcgctggc cagcacggtg agggtcgctg gctcgccggc
       61 gtcggcgcag gccttggcga cgtcgagcac ggcggccgtc accgtgctgg ccaggctggc
      121 tccgggtcgg gcgcggtggt agcggacgag ggtgaggaac agcacgaccg agtcggtcag
      181 cgtgcccgcg gcgtggtaga ggtcgtcggg cagggcgtcc agcagccgcc ggccgaccgg
      241 gccgcggaac ccgccgacgc ggccgttgtg gacgacggcc agcgtgccgt gggcaaacgg
      301 ggcggccgcg tcgacgccgt agggcagccc aggcgtgccc gagcggacgg cggccagctg
      361 taccacgccg cgtagccgcc cggccaggtg gagcaggttg gggtcggacc agggcgggcg
      421 ctcggtggcg tagcggaccg gctcgcgctc gcccacgggc caccagacga ccccggtgcc
      481 gtcgacgttc acaaccccgt cgtgctgcag ccggggctgg taggcctgca cggacagcga
      541 atgcggcggg tcgctgagca gggtcgacag cggggcaggg gggccgaggt agccagccat
      601 gcggcacatc agcgcacatc ccaggccagc cgcaggccgg cgaagagctg tcggcgctgc
      661 gggtagtccc agttgcggaa ggtggtgcgg gctacccagc gggaggtcgc ccaggaggcg
      721 ccccgcagga ccaggtagtc atcgccgaag aagacctcgc tgtactcggg gtaggggaag
      781 ctggtgtagc ccgggtagcc ggtgaacggc gagcaggtcc actcgtagac gtcgccgagg
      841 agctgctcga cgccgtaggc cgaggcgccc gccgggtagc tgccgaccgg cgccggtccc
      901 caggcggcga tgtccaggtt ggcgcgctcc ggcccggggg gcgcctcgcc ccacgggtag
      961 gtgcggctgc ggcgggcgag cgggtcccag gcggcggcct tctcccactc ggcctcgctc
     1021 ggcagccggg agccggccca gcgcgcaaac gccaatgcct cgtgggcgga cacgtgctgg
     1081 acgggctcgc gggggtccag cggcaggacg tggtcgaagc gccgcaccag ccagccgcct
     1141 gacccgtccg acagccagcc ctgcggggcg cggtggccgg tctccagccg ccaggcccag
     1201 cccgcctcgg tccaatgctc ggggtggtcg tagccgccgg cggcgacgaa ggcggcgtag
     1261 cggcgggcgg tgaccgggaa ccggtcgatc gcgaaggtcg ccacgtcgac ccggtgagct
     1321 ggccgctcgt tgtcgtaggc ggcggtgcgg tcgtcggtgc ccatgtcgaa cggcccgccc
     1381 gggacgacca cgcgctcggc atcgtcgacc gggcggcgca gcgccgggcg cggccgggcg
     1441 gccagcgggt agggccgggc gtcggagcgc aggtcgaggg cctgcagcat cgtctcctgg
     1501 tgctgcgcct catgctgggt gatcatccgg tacacgtagc cggccgcgag caggggacgg
     1561 tcgggatcga agctgatcga ccgcaggatc gacaggacgt ctccgcggac gtcggccagg
     1621 taggcgacca cctcgtcgcg gggcaggatc ggcaggtcgg cgcggaccca gcgggggttc
     1681 tcgaaggggt tgtagacctg gtccagggtg gggtcgtggg cggctcggcc gtccagctcg
     1741 cgcagcagcc agagctcctc gaagttgccg acgtgcccga cgtcccagac gagcgggctc
     1801 atgatcgggt cgtactggcg gtgcagctca cgttcgtcga gcggcgc
//
LOCUS       DASPYY010000781         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2238778, whole genome shotgun sequence.
ACCESSION   DASPYY010000781 DASPYY010000000
VERSION     DASPYY010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238778"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VEP73_12180"
     CDS             <1..1427
                     /locus_tag="VEP73_12180"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HYT27230.1"
                     /translation="KLNSALAATPIPGSTQFTVSYADDDAALAERVLRDYAKSYAGRR
                     NAKYAAQLTAVRTPRVREIQALTQQAQSLSRQANAERRASANGTASPGTEAQLTYMTN
                     LLINKQTELGDIDKQAALLGTPTSFDDPVNVEPQSNPAGRGVALAIGLLLGLLGGIGA
                     AFVAEAYAPKVLSADDVESATDLTVIADVPHLPRRQRRKGVAVLDRPFSPTAEGYRRL
                     VGALERRGLGTSVKVLAILSADAHEGKSTVAVNLAHVLARLGRNVVLVSSDLRRPGIE
                     KLLGLPGSEGLAGYLEGERNDLASLFISVSDNLVVLPAGASKSNPADLLATKQVGAML
                     KLLRATGMIVILDTPPARWSADAISLAGAADASLLVARAGRASTKSIREVAEGLRREG
                     LRTLGSILLGSKTPKLTRLMRYGHYASQEGYSDAVQARMRSVPPAGGNGGAAGLSLPV
                     RKDRPAENGVEREGRKALGGPPGK"
     gene            1512..>2486
                     /locus_tag="VEP73_12185"
     CDS             1512..>2486
                     /locus_tag="VEP73_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT27231.1"
                     /translation="MNGHVFVTGVAGFIGSHLAEALLAEGHEVVGVDALTPYYAPVEK
                     RANLAGPLGRPGFRLVEGDLASIDLDGWLDGAAAVFHQAAQPGVRASWGADFAIYVHH
                     NVLATQQLLEACVRTGVPRLVAASSSSVYGDAPTYPTTEESTTRPVSPYGVTKLASEH
                     LCLAYAQAAVSSMTVATLRYFTVYGPRQRPDMGFRRFFEAAHAGRPVTVYGDGEQTRD
                     FTFVSDVVRANLLAMHAPIHAEAVNVGGGRRVTLNEVLELVGRAAGRRLEVVREPARP
                     GDARHTGADGTRAEALLGYRPEVDLEEGLAAQAAWVADRYGLGVRRGAT"
BASE COUNT          338 a          967 c          882 g          299 t
ORIGIN      
        1 ccaagctgaa ctcggccctc gccgcgaccc cgatcccggg ctcgacccag ttcaccgtct
       61 cctacgccga cgacgacgcg gccctggccg agcgggtcct gcgcgactac gccaagtcct
      121 acgcgggacg ccgcaacgcc aagtacgccg cgcagctcac cgcggtccgc acgccccggg
      181 tgcgcgagat ccaggcgctc acccagcagg cccagagcct ctcgaggcag gcgaacgccg
      241 agcgcagggc gagcgccaac ggcaccgcct ccccgggcac cgaggcgcag ctcacctaca
      301 tgaccaacct gctcatcaac aagcagaccg agctcggcga catcgacaag caggcggcgc
      361 tgctcggcac gcccaccagc ttcgacgacc cggtcaacgt cgagccgcag agcaacccgg
      421 ccgggcgcgg ggtggcgctc gccatcggcc tgctgctcgg gctgctcggc ggcatcggcg
      481 cggcgttcgt cgccgaggcg tacgccccga aggtcctgtc cgccgacgac gtcgagtcgg
      541 cgaccgacct gaccgtgatc gcggacgtcc cccacctgcc gaggcgccag cgccgcaagg
      601 gcgtcgcggt cctcgaccgg ccgttcagcc cgaccgccga gggctaccgc cggctggtcg
      661 gcgcgctcga gcggcgcggg ctcggcacca gcgtgaaggt cctcgccatc ctctcggccg
      721 acgcccacga ggggaagtcg accgtcgcgg tgaacctcgc ccacgtgctc gcccgcctgg
      781 gccgcaacgt cgtgctcgtc tcgagcgacc tgcgccggcc cgggatcgag aagctgctcg
      841 ggctgcccgg gtccgagggc ctcgccggct acctggaagg cgagcgcaac gacctcgcgt
      901 cgctgttcat ctcggtgagc gacaacctcg tcgtgctccc agccggcgcc agcaagagca
      961 acccggccga cctgctcgcc accaagcagg tgggggcgat gctcaagctc ctgcgggcga
     1021 cgggcatgat cgtgatcctc gacaccccgc cggcgcgctg gtcggccgac gcgatctccc
     1081 tcgccggcgc ggccgacgcc tccctgctcg tcgcccgcgc agggcgcgcc tcgaccaagt
     1141 cgatccgcga ggtcgccgag gggctgcggc gcgaggggct gcggacgctc gggtcgatcc
     1201 tgctcggctc gaagacgccg aagctcaccc ggctcatgcg gtacgggcac tacgcctcgc
     1261 aggagggcta cagcgacgcc gtccaggcgc gcatgcgcag cgtgcccccg gcgggcggca
     1321 acggcggggc ggcgggcctg tccctgccgg tccgcaagga ccgcccggcg gagaacgggg
     1381 tcgagcggga gggacgcaag gcgctcggcg ggccgcccgg caagtagccc ggctcccgtg
     1441 cccgccgctt ccggtccgga cgagcccagc gccgaggtgc ggcgggctct cgacagcttg
     1501 gggtacctcc aatgaacggg catgtgttcg tcaccggcgt cgccggcttc atcgggtcgc
     1561 acctggcaga ggcgctgctc gccgaggggc acgaggtcgt gggcgtcgac gcgctgaccc
     1621 cctactacgc ccccgtcgag aagcgggcca acctggccgg accgctcggc cggcccggct
     1681 tccgcctggt cgagggcgac ctcgcctcga tcgacctcga cggctggctc gacggggccg
     1741 ccgccgtgtt ccaccaggcc gcccagccgg gcgtgcgcgc ctcctgggga gccgacttcg
     1801 ccatctacgt ccaccacaac gtcctcgcca cccagcagct gctcgaggcg tgcgtgcgca
     1861 ccggggtgcc gcggctcgtc gcggcctcct cgtcgagcgt ctacggtgac gcgccgacct
     1921 acccgaccac cgaggagtcg accacccgcc cggtctcgcc gtacggtgtc accaagctcg
     1981 cgagcgagca cctgtgcctg gcgtacgcgc aggcggcggt gtcctcgatg accgtggcga
     2041 ccctgcgcta cttcaccgtc tacggcccgc gccagcgccc cgacatgggc ttccggcgct
     2101 tcttcgaggc cgcccacgcc ggccggccgg tcaccgtgta cggcgacggg gagcagaccc
     2161 gcgacttcac cttcgtgtcc gacgtcgtcc gcgccaacct gctcgccatg cacgcgccga
     2221 tccacgccga ggcggtgaac gtcggcggcg ggcggcgggt gaccctgaac gaggtgctcg
     2281 agctggtcgg ccgggcggcg gggcggaggc tcgaggtcgt cagggagccc gcgcggccgg
     2341 gcgacgcgag gcacaccggg gcggacggca cccgggccga ggcgctgctc ggctaccggc
     2401 cagaggtcga cctcgaggag gggctggccg cccaggccgc ctgggtcgcc gaccggtacg
     2461 gcctcggcgt ccgcagaggc gcgacg
//
LOCUS       DASPYY010000782         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_130150, whole genome shotgun sequence.
ACCESSION   DASPYY010000782 DASPYY010000000
VERSION     DASPYY010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130150"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="lipA"
                     /locus_tag="VEP73_12190"
     CDS             complement(<1..862)
                     /gene="lipA"
                     /locus_tag="VEP73_12190"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018826692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYT27232.1"
                     /translation="MFPRTPAPPPTDATFARKPPWLKVRASMGPNYTELKHLMRDAEL
                     HTVCEEAGCPNIYECWEAREATFLIGGEHCTRACPFCQIQNGRPRGYDTDEPLRVAHA
                     VARMGLRFAVITGVARDDLADGGAWLFAESVRQVRALLPGCGVEVLVPDFRGSPDALR
                     QVADALPDVLGHNVETVPRLYKRIRPGFTYAGSLELLARARAWLPAGHVTKSNLILGM
                     GEERGEVLATMRDLRDAGVELLTVTQYLQPSREHLNLQRFVPPEEFAELAEAARALGF
                     AHVESGPLVRS"
     gene            complement(939..1709)
                     /gene="lipB"
                     /locus_tag="VEP73_12195"
     CDS             complement(939..1709)
                     /gene="lipB"
                     /locus_tag="VEP73_12195"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYT27233.1"
                     /translation="MEPLWLVRAGTVPYRRAWAWQRALVERRAAGEIPDTALLLEHPH
                     VYTIGKRGSGADVLASPEWLAAQGAEVVRSDRGGQVTYHGPGQLVGYPVTRLDPEPDV
                     WRFVRRVEDAMVAVAADFGLEARGESGLLTGVWVEDAKLVAIGMRVSRGVTSHGFALN
                     CATDLAKFAAIVPCGMPDRAACSLSSALGRTVTVADALPVVERRLAEALDRAPVEVDA
                     ATLDLPATDHPPEPDFAPHPLRLASSASGLPAPETTNA"
     gene            complement(1713..>3051)
                     /gene="sucB"
                     /locus_tag="VEP73_12200"
     CDS             complement(1713..>3051)
                     /gene="sucB"
                     /locus_tag="VEP73_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HYT27234.1"
                     /translation="VEADEPLFEVSTDKVDTEVPSPVAGILTSIKVKEDETVEVGAEL
                     AVVSTGGAGGGTAEARPREAEEAARAAGEERDGEERGAREEARPAAAAGDGAAGDGAG
                     GAVVSPLVRRLAREHGIDLRRVKGSGSGGRVRREDIEAYLERQGGERAEAPAAPAAAA
                     REEAPAPRTGDGRAAPAPAPAREGRPAAGAPPKQATPLATGVREQVAPATRTRKVIAE
                     RMVTSLQTSAQLTAAVEIDMTKVMRLRAQAKDAFKAREGVSLSPLPFSLLAVIQAIRA
                     YPNANSALDEDMDLHVYQDVNLGIAVDTPKGLFVPVIRNADQLNLSGLARAVADVARR
                     TREGKVSPNELSGSTFTVTNTGSVGTVWDTPIINQPNAAIFATPAIVKRPVVVDDPEL
                     GEVVAVRHMMYGILTYDHRILDGADAARFLQKVKQALEAGDFARELGLDRDEG"
BASE COUNT          360 a         1131 c         1154 g          406 t
ORIGIN      
        1 tcgagcgcac cagcgggccc gactcgacgt gggcgaaccc gagcgccctg gccgcctcgg
       61 cgagctcggc gaactcctcg ggcgggacga accgctgcag gttgaggtgc tcgcgggacg
      121 gctgcaggta ctgcgtgacc gtgagcagct ccaccccggc gtcgcgcagg tcgcgcattg
      181 tcgcgagcac ctcgccgcgc tcctcgccca tcccgaggat caggttggac ttggtgacgt
      241 gcccggccgg cagccacgcg cgggcgcggg cgagcagctc gagcgagccg gcgtaggtga
      301 agccgggccg gatccgcttg tacaggcggg gcacggtctc gacgttgtgc ccgagcacgt
      361 cgggcagcgc gtccgcgacc tgcctgaggg cgtcggggct gccacggaaa tcggggacga
      421 gcacctctac cccgcagccg ggcagcagcg cgcggacctg gcggaccgac tcggcgaaca
      481 gccacgcccc gccgtcggcc aggtcgtccc gggcgacgcc ggtgatgacc gcgaagcgca
      541 ggcccatgcg ggcgaccgca tgggcgaccc ggagtggctc gtcggtgtcg tacccccgcg
      601 gccggccgtt ctggatctgg cagaacgggc aggcccgggt gcagtgctcc ccgccgatga
      661 ggaaggtggc ctcgcgcgcc tcccagcact cgtagatgtt cgggcagccg gcctcctcgc
      721 agaccgtgtg cagctcggcg tcccgcatca ggtgcttcag ctcggtgtag ttcggcccca
      781 tgctggccct gaccttgagc cacggcggct tgcgggcgaa ggtcgcgtcc gtgggcggcg
      841 gcgccggggt gcgcgggaac accgagaggg tccgcgcgcc ggacgggatc accggctccg
      901 aggctacggg gctggcaggc tcaggcacct cgacgggctc aggcattggt agtctccggg
      961 gccgggagcc ccgaggcgct cgaggcgagc ctcaggggat gcggcgcgaa gtcgggttcc
     1021 ggcgggtggt cggttgccgg caggtccagg gtagcggcat ccacctcgac cggcgcgcgg
     1081 tcgagcgcct cggcgagccg gcgctcgacc acgggaaggg cgtcggcgac ggtgaccgtc
     1141 cgcccgagcg cgctggacag ggagcaggcc gcgcggtccg gcatcccgca ggggacgatg
     1201 gcggcgaact tggcgaggtc ggtggcgcag ttgagggcga agccgtggct cgtgaccccc
     1261 cgcgagacgc gcatgccgat cgcgacgagc ttcgcgtcct cgacccagac gccggtgagg
     1321 aggcccgact cgccgcgcgc ctcgagcccg aagtcggccg ccacggcgac catggcgtcc
     1381 tccacccggc gcacgaagcg ccagacgtcc ggctccgggt cgagacgggt gacggggtag
     1441 ccgacgagct gcccgggccc gtgataggtg acctgcccgc cgcggtcgct gcggaccacc
     1501 tcggcgccct gggcggcgag ccactccggg ctcgcgagca cgtccgcgcc cgagccgcgc
     1561 ttgccgatgg tgtacacgtg cgggtgctcg agcagcagcg cggtgtcggg gatctcgccg
     1621 gccgcccggc gctcgaccag cgctcgctgc caggcccagg cgcgccggta cggcaccgtg
     1681 ccggcgcgca cgagccagag cggctccatc ggctacccct cgtcccggtc cagcccgagc
     1741 tcacgggcga agtcgcccgc ctcgagcgcc tgcttgacct tctggaggaa gcgggccgcg
     1801 tcggcgccgt ccaggatgcg gtggtcgtag gtgaggattc cgtacatcat atggcgcacc
     1861 gcgacgacct cgccgagctc cgggtcgtcg acgaccacgg ggcgcttgac gatcgccggg
     1921 gtggcgaaga tcgcggcgtt cggctggttg atgatcggcg tgtcccagac cgtcccgacg
     1981 ctgccggtgt tcgtgacggt gaaggtcgag cccgacagct cgttcgggct caccttgcct
     2041 tcccgggtgc gcctggccac gtcggccacg gccctggcca gcccggacag gttgagctgg
     2101 tcggcgttgc ggatcacggg cacgaacagg cccttcgggg tgtcgaccgc gatgccgagg
     2161 ttcacgtcct ggtagacgtg gaggtccatg tcctcgtcga gcgccgagtt cgcgttcggg
     2221 taggcccgga tggcctggat caccgcgagg agcgagaacg gcagcggcga gaggctgacg
     2281 ccctcgcgcg ccttgaaggc gtccttggcc tgcgcgcgca gccgcatcac cttggtcatg
     2341 tcgatctcga ccgccgcggt gagctgcgcg ctcgtctgca gcgaggtgac catccgctcg
     2401 gcgatgacct tgcgcgtccg ggtcgccggg gcgacctgct cgcggacgcc ggtcgcgagc
     2461 ggggtcgcct gcttcggggg cgcgccagcc gccggcctgc cctccctggc cggggcgggc
     2521 gcgggcgccg ccctgccgtc gcccgtccgg ggggcgggcg cctcctcccg ggccgccgcg
     2581 gccggcgcgg ccggcgcctc ggcccgctcc ccgccctgcc gctcgaggta cgcctcgatg
     2641 tcctcgcggc gcacccgccc gcccgacccg ctgcccttga cccggcgcag gtcgatgccg
     2701 tgctcgcggg cgagccggcg caccagcggc gagacgaccg cgccgcccgc gccgtcgccc
     2761 gccgcgccgt cgcccgcggc agcggccggc ctggcctcct cccgggcgcc gcgctcctcg
     2821 ccgtcgcgct cctcgccagc ggcccgggcg gcctcctcgg cctccctggg tcgggcctcg
     2881 gccgtcccgc cgccggcgcc gccggtcgag accacggcca gctcggcgcc gacctcgacg
     2941 gtctcgtcct ccttcacctt gatcgaggtg aggatgcccg ccacggggga ggggacctcg
     3001 gtgtcgacct tgtcggtcga cacctcgaac agcggctcgt cggcctcgac c
//
LOCUS       DASPYY010000783         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_272660, whole genome shotgun sequence.
ACCESSION   DASPYY010000783 DASPYY010000000
VERSION     DASPYY010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272660"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /gene="pheT"
                     /locus_tag="VEP73_12205"
     CDS             complement(<1..1876)
                     /gene="pheT"
                     /locus_tag="VEP73_12205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYT27235.1"
                     /translation="MRVPLSWLADHVDVGVSATTLADRLTAAGVEVETVDRVGEDVAG
                     VHTARVLEVGRHPNADRLALVRIDAGGDERMVVCGAHNYAPGDVVAWAAPGAKLPGGV
                     DIGRRQVRGVTSDGMLASARELGVLDDHAGILVLPPDTPAGADFVEAAGLRDAVLEIK
                     TYPNRGDTLSIRGVAREAALALGADLKPLDLTVPESGPPVASLASVEVLDTEGCPLYA
                     ARVVEGLDATRPAPLWMARRLWLYGQRPLGAVVDVTNYLLIDQGQPLHAFDLDRVPDR
                     RIVVRRAGEGERLRTLDGRERVLDGGDTLITSGERALALAGIMGGEESEVRADTRRVL
                     IESAHFPPATVRRTLRRLGMSTEGAQRWVRGVDPAGAAPVADQAAALMARLAGGAVAG
                     GRLEAGPGVPQRPAVRLDWARSATRLGAPSDPEFAAGYLRGVGCLTEVTDAATVLAVP
                     PSWRFDIEGWADLEEEVARRWGYENLPATLPRATGGRLTQEQRLRRGVREALAGMGLT
                     EAQTSPFLSQAAFDRLGLAADDPRRSTLRLANPLSEEAPELRTTLLPGLADAARRNLA
                     RGLGGVALLELGLVFLPDPDPPRALGGLGGGDEALPDQPLVLGLLLTGQHPAGRWDDP
                     R"
     gene            complement(1888..2946)
                     /gene="pheS"
                     /locus_tag="VEP73_12210"
     CDS             complement(1888..2946)
                     /gene="pheS"
                     /locus_tag="VEP73_12210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYT27236.1"
                     /translation="MSTATPDPGVDAIVEAGLAAVAAAGDLDALRAAEQRHVSRRSPL
                     GDLQRSLGDLDPELRRALGRRVNQARAELTAALAARQAGLESARDAALLEAERVDVTL
                     PGRVPPRGSVHPLSRTVDEVVDIFVGLGYRVAEGPEVETDWYNFEALNFAPGHPARLM
                     HDTLFVEPDPAAPSPLLLRTHTSPVQIRTMLAQPPPVYVVIPGRCYRADTPDATHVPV
                     FNQIEGLAVAEDITMGDLKGTLQAFARAYFGDERDIRLRPAYFPFTEPSAEIDVSCFI
                     CGGRGGDCRTCKGEGWIEILGAGMVHPRVLANVGYDPDKVSGFAFGMGVERIYMLRSG
                     LADMRTLGDNDVRWLASA"
     gene            complement(3149..4216)
                     /locus_tag="VEP73_12215"
     CDS             complement(3149..4216)
                     /locus_tag="VEP73_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT27237.1"
                     /translation="MADTEAYLEALPDAVVVVEADGTVAWANVAAERLAGTRREELAG
                     KALDGVLPLLDGAGNPWWACSERLRRMPGVRGVPPRELTLAAGGRAAPVDLSATFVRG
                     ADTSVVQAVCVLRDASARRRTDANRGELISTLAHELRSPLTSVKGFTSTLLHRWDRFG
                     DEQKRHMLTTINADADRVTRLIGELLDVSRIEAGRLELQRQMIEVPLIARDVVGRFEL
                     QSDRHAFRLAFPPGFPEVYADRDKVEQVLVNLVENAVKYSKGGTVTVRGEVADDAVEV
                     AVADEGVGIPADQLPLIFTKFYRRGGHGAPTGTGLGLFITRGLVEAHGGRIWARSEVG
                     RGTEVRFRLPRGGLELAGIAP"
     gene            complement(4209..>4508)
                     /locus_tag="VEP73_12220"
     CDS             complement(4209..>4508)
                     /locus_tag="VEP73_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYT27238.1"
                     /translation="EWRRLAGALADRGVALVGADPHAGDDTDAAPLDRPVAIVLGNEA
                     HGLGAEARRDLHHLVRLPTYGRAESLNLAAAAAVLLYETARRRRAAAREEAAAVG"
BASE COUNT          559 a         1642 c         1777 g          530 t
ORIGIN      
        1 gccgcgggtc gtcccagcgt ccggcggggt gctggcccgt gaggagcagc ccgaggacga
       61 gcggctggtc cggcagcgcc tcgtcgcccc cgccaagccc ccccagggca cggggcgggt
      121 cggggtcggg gaggaagacg aggccgagct cgaggagggc gaccccgccg agcccgcggg
      181 cgaggttgcg cctcgccgcg tcggcgagcc ctggcaggag cgtggtgcgc agctccgggg
      241 cctcctccga cagcgggttc gcgaggcgca gcgtgctgcg gcgggggtcg tcggcggcca
      301 ggccgaggcg gtcgaacgcg gcctgggaga ggaacggtga ggtctgcgcc tcggtcaggc
      361 ccatgccggc cagcgcctcg cggacgccgc ggcgcagccg ctgctcttgg gtgagccggc
      421 cgccggtcgc cctcggcagg gtggccggca ggttctcgta gccccagcgc cgcgccacct
      481 cctcctcgag gtcggcccac ccttcgatgt cgaagcgcca gctcggcggg accgcgagca
      541 cggtcgcggc gtcggtgacc tcggtcaggc agccgacacc gcgcaggtag ccggcggcga
      601 actcggggtc gctcggggcg ccgagccggg tcgccgaccg cgcccagtcg agccggacgg
      661 cggggcgctg gggcacgccg ggaccggcct cgaggcggcc gcccgccacc gcgccgcccg
      721 cgaggcgggc catgagcgcg gcggcctggt cggcgacggg cgcggccccg gccgggtcga
      781 cgccccgcac ccagcgctgg gcgccctcgg tgctcatgcc gaggcggcgc agggtgcgcc
      841 ggacggtcgc gggcgggaag tgggccgact cgatgagcac ccgccgcgtg tccgcgcgga
      901 cctcggactc ctcgccgccc atgatcccgg cgagggcgag cgcccgctcg cccgaggtga
      961 tcagggtgtc gccgccgtcg aggacccgct cgcggccgtc cagcgtgcgc aggcgctcgc
     1021 cctcgccggc ccggcgcacg acgatgcgcc ggtcgggcac ccggtcgagg tcgaaggcgt
     1081 gcagcggctg gccctggtcg atcagcaggt agttggtcac gtccacgacc gcgccgagcg
     1141 gccgctggcc gtacagccag aggcgccttg ccatccacag cggagcggga cgcgtggcgt
     1201 ccaggccctc gacgacccgg gcggcgtaga gcgggcagcc ctcggtgtcg agcacctcga
     1261 ccgaggcgag gctcgcgacc ggcggcccgc tctcgggcac ggtgaggtcg agcggcttca
     1321 ggtcggcgcc cagcgcgagc gcggcctccc tggccacccc gcggatcgac agggtgtcgc
     1381 cccggttcgg gtaggtcttg atctcgagca cggcgtcgcg caggccggcc gcctcgacga
     1441 agtccgcccc ggccggcgtg tccgggggca gcaccaggat gccggcgtgg tcgtcgagca
     1501 cgccgagctc gcgggccgag gccagcatgc cgtcggaggt gacgccgcgg acctgcctgc
     1561 ggccgatgtc gaccccgccg ggcagcttgg cccctggcgc ggcccaggcg acgacgtcgc
     1621 cgggggcgta gttgtgggcg ccgcacacga ccatgcgctc gtcgcccccg gcgtcgatgc
     1681 gcacgagcgc gaggcggtcg gcgttcgggt gcctgccgac ctcgagcacg cgggcggtgt
     1741 ggacgccggc gacgtcctcc cccacccggt cgacggtctc gacctcgacc ccggcggcgg
     1801 tgagccggtc ggccagcgtc gtggccgaga cgccgacgtc cacgtggtcg gccagccagg
     1861 acaaggggac gcgcatcagc cctccattca ggcgctggcc agccagcgca cgtcgttgtc
     1921 gcccagggtc cgcatgtcgg cgagtcccga gcggagcatg tagatccgct ccactcccat
     1981 gccgaacgcg aagccggaca ccttgtcggg gtcgtagccg acgttcgcga gcaccctggg
     2041 gtggaccatg ccggcgccga ggatctcgat ccagccctcg cccttgcagg tgcggcagtc
     2101 gccgccccgg ccgccgcaga tgaagcagga gacgtcgatc tcggcgctcg gctcggtgaa
     2161 ggggaagtag gcgggccgga gccggatgtc ccgctcgtcg ccgaagtacg cgcgggcgaa
     2221 cgcctgcagg gtccccttga ggtcgcccat ggtgatgtcc tcggcgacgg ccaggccctc
     2281 gatctggttg aacaccggca cgtgggtcgc gtccggggtg tcggcccggt agcaccggcc
     2341 cgggatcacc acgtagacgg gcggtggctg ggccagcatc gtgcggatct gcaccggcga
     2401 ggtgtgggtg cgcagcagga gcgggctcgg ggcggccggg tcgggctcga cgaacagcgt
     2461 gtcgtgcatg agccgggccg ggtggccggg cgcgaagttc agcgcctcga agttgtacca
     2521 gtcggtctcg acctcggggc cctcggcgac gcggtagccg aggccgacga agatgtccac
     2581 gacctcgtcg acggtgcggc tcagggggtg gacgctcccg cgcgggggga cgcggccggg
     2641 gagggtcacg tcgacccgct cggcctcgag cagcgcggcg tccctggccg actcgagccc
     2701 ggcctggcgg gcggcgaggg cggcggtgag ctcggccctg gcctggttga cgcgccggcc
     2761 gagcgccctg cggagctccg ggtcgaggtc gccgagcgag cgctgcaggt cgccgagcgg
     2821 cgaccggcgg gagacgtgcc gctgctcggc ggcgcggagc gcgtcgaggt cgccggcggc
     2881 ggcgaccgcg gcgaggccgg cctcgacgat cgcgtcgaca ccggggtcgg gcgtggcggt
     2941 ggacatggga gctcctcatc ggaggcacgc ctcggcgtgc cgaccgggac agggacatcg
     3001 gaggcacgcc tcggcgtgcc gaccgggaca gggacaggga cagggacagg gacagggaca
     3061 gggacaggag atgcacggca agcttgtcgt ccgctcggcg gtcgccccgc cggggcccac
     3121 gtggagccgg cgcctcgcgc aattcaggct atggcgcgat cccggccagc tccaggcccc
     3181 cgcggggcag gcgaaatcgc acctcggtgc cgcgaccgac ctcggagcgg gcccagatgc
     3241 ggccgccgtg ggcctcgacg aggccccggg tgatgaacag gccgagcccg gtgccggtgg
     3301 gcgcgccgtg gcccccccgc cggtagaact tggtgaagat gagcgggagc tggtcggccg
     3361 ggatgccgac accctcgtcg gccacggcga cctcgacggc gtcgtcggcg acctcgccac
     3421 gcaccgtcac ggtcccgccc ttggagtact tcaccgcgtt ctccaccagg ttcacgagca
     3481 cctgctcgac cttgtcgcgg tcagcataga cctcggggaa gccgggaggg aacgcgagcc
     3541 ggaaggcgtg acggtcggac tggagctcga accggccgac cacgtccctg gcgatgagcg
     3601 ggacctcgat catctggcgc tgcagctcga gccggcccgc ctcgatccgg gacacgtcga
     3661 gcagctcgcc gatcagccgg gtgacgcggt cggcgtcggc gttgatcgtg gtcagcatgt
     3721 ggcgcttctg ctcgtcgccg aagcggtccc agcggtgcag cagcgtcgag gtgaacccct
     3781 tgacgctggt gagcggggac cgcagctcgt gggcgagggt ggagatgagc tcgccgcggt
     3841 tggcgtcggt ccgccgccgg gccgacgcgt cgcgcaggac gcagaccgcc tgcaccaccg
     3901 acgtgtcggc accgcggacg aaggtggccg acaggtcgac gggcgcggcg cgcccgcccg
     3961 ccgccagggt cagctcgcgg gggggcaccc cgcggacccc tggcatccgg cggagccgct
     4021 cggagcacgc ccaccacggg ttgcccgccc cgtcgagcag gggcaggacg ccgtcgagcg
     4081 ccttgccggc cagctcctcc ctgcgcgttc cggccaggcg ctccgcggcc acgttggccc
     4141 aggccacggt gccgtccgcc tcgacgacga cgaccgcgtc gggcagagcc tcgaggtatg
     4201 cctcggtgtc agccaaccgc cgccgcctcc tcccgcgcgg cagcccgccg cctgcgcgcc
     4261 gtctcgtaca ggagcaccgc ggccgcggcg gccaggttca ggctctccgc ccgcccgtag
     4321 gtcggcagcc ggacgaggtg gtggaggtcg cggcgggcct cggcgccgag gccatgcgcc
     4381 tcgttgccga gcacgatcgc gaccgggcgg tcgagaggcg ccgcgtcggt gtcgtcgccc
     4441 gcgtgcgggt cggcgcccac cagtgccacc ccccggtcgg cgagcgcccc ggccagccgc
     4501 cgccactc
//
LOCUS       DASPYY010000784         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1818309, whole genome shotgun sequence.
ACCESSION   DASPYY010000784 DASPYY010000000
VERSION     DASPYY010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818309"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VEP73_12225"
     CDS             <1..814
                     /locus_tag="VEP73_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYT27239.1"
                     /translation="SAYTRMSGADLDGRSIRKGAAEAVVKWVREQGGKIPLELEPIVD
                     RIARDGGTPLVVASNDQVLGVIQLKDVVKGGMRDRFDQLRRMGIRTVMITGDNPLTAA
                     AIASEAGVDDFLAQADVGVAMNTGTVAAKEAGNMVDLDSNPTKLIEIVEIGKQLLITR
                     GSLTTFSIANDVAKYFAIIPALFASTFPVLDRLNVLRLETPRSAVLSAVIFNALIIIA
                     LVPLALRGVRFRPVSATALLRRNLLVYGLGGIVLPFIGIKLIDVVIHLVRLA"
     gene            832..1428
                     /gene="kdpC"
                     /locus_tag="VEP73_12230"
     CDS             832..1428
                     /gene="kdpC"
                     /locus_tag="VEP73_12230"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HYT27240.1"
                     /translation="MLTDIRRGAVALVAFTVVCGLAYPLLMLGVGQLAFRGAAQGSLV
                     ERDGRVVGSALIGQSFTDPRYFWGRPSAAGSDGYDPTASGASNLGPTSRKLADTVRQR
                     VAALLKANPGKTAAEIPSELVTASGSGLDPDISPAAAEFQVERVALARGLAAARVRAL
                     VREHTEGRELGVLGEPRVNVLELNLALDGGGPPVTGGR"
     gene            1481..2437
                     /locus_tag="VEP73_12235"
     CDS             1481..2437
                     /locus_tag="VEP73_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYT27241.1"
                     /translation="MTVHDRLHVPSRGRLKIFLGAAAGVGKTYAMLGEARDLKAVGVD
                     VVIGYLEPHGRRTTEQRAAGLEVAPRRIARVRDRPFAETDVDWLLERSPQLCLVDELA
                     HTNHPGGRHAKRWQDVEELLAAGIDVFTTVNIQHLESLNDKVLEVTGVRVRETFPDRL
                     LHEADEVVLVDLPPEALRERVMVCIDGRPDSSGLVRRAARMARRSSGRLWMLHVEPAD
                     ERESSQVHEMVDEAEALAGRLGATVLRRAGDVAEVVVEAARELGITQIVLGESHRPRW
                     KELVGRSIIARILRETDGVDVHIIADHRARPPRPTTRTAEEG"
BASE COUNT          351 a          976 c          902 g          358 t
ORIGIN      
        1 cagcgcgtat acccgcatgt cgggcgcgga cctggacggg cgctccatcc gcaagggcgc
       61 cgccgaggcg gtcgtcaaat gggttcggga gcagggcggc aagatcccgc tcgagctgga
      121 gccgatcgtc gaccggatcg cccgcgacgg cggcacgccg ctggtggtcg cctccaacga
      181 ccaggtgctc ggcgtgatcc agctcaagga cgtggtcaag ggcggcatgc gcgaccgttt
      241 cgaccagctc cgccgcatgg gcatccgcac ggtgatgatc accggcgaca acccgctcac
      301 cgcggccgcc atcgccagcg aggccggcgt ggacgacttc ctcgcccagg ccgacgtcgg
      361 cgtggccatg aacaccggca cggtggcggc caaggaggcc ggcaacatgg tcgacctcga
      421 ctccaacccg accaagctga tcgagatcgt cgagatcggc aagcagctgc tcatcacccg
      481 cgggtcgctg accaccttct cgatcgccaa cgacgtcgcc aagtacttcg cgatcatccc
      541 ggcgctgttc gcgtcgacct tcccggtgct cgacaggctg aacgtcctgc gcctcgagac
      601 gccgcggtcg gcggtcctct ccgccgtcat cttcaacgcc ctgatcatca tcgccctggt
      661 gccgctggcc cttcgcggcg tgcgcttccg gccggtctcc gccaccgccc tgctccgccg
      721 caacctgctc gtctacggcc tcggcggcat cgtgctgccc ttcatcggca tcaagctgat
      781 cgacgtcgtc atccacctgg tccgcctcgc ctgagcccag gagcccgtcc catgctcacc
      841 gacatccgcc gaggggccgt cgccctggtc gcgttcaccg tcgtctgcgg gctcgcctac
      901 ccgctgctca tgctcggcgt cggccagctc gccttccgcg gcgccgccca gggcagcctg
      961 gtcgagcggg acggccgggt ggtcgggtcc gccctgatcg gccagtcgtt caccgacccc
     1021 aggtacttct ggggccgccc gtcggccgcc ggctccgacg gctacgaccc gaccgcctcg
     1081 ggcgcgtcca acctcggccc cacctcgcgg aagctcgccg acacggtcag gcagcgggtc
     1141 gccgccctcc tcaaggccaa cccgggcaag accgccgccg agatcccctc cgagctggtc
     1201 accgcgtccg gctccgggct cgacccggac atctccccgg ccgccgccga gttccaggtg
     1261 gagcgggtcg ccctggcgcg cggcctcgcc gccgcccggg tccgcgcgct cgtgcgcgag
     1321 cacaccgagg ggcgggagct cggcgtgctc ggcgagccgc gggtgaacgt gctggagctg
     1381 aaccttgccc tcgacggtgg aggccctccg gtcaccggag gccggtgacc aggagctacg
     1441 ctggcgaccg gccgcccgcg cggccatgct gggggccacc atgaccgtcc acgaccggct
     1501 gcacgtgccg agccgcgggc gcctgaagat cttcctgggc gccgcggccg gcgtcggcaa
     1561 gacgtacgcg atgctcggcg aggcccgcga cctgaaggcc gtcggcgtcg acgtggtgat
     1621 cggctacctg gagccgcacg ggcgccgcac gaccgagcag cgggcggccg ggctcgaggt
     1681 ggcgccgcgc cgtatcgcca gggtcaggga ccgtccgttc gcggagacgg acgtcgactg
     1741 gctgctcgag cggtcgccgc agctctgcct ggtcgacgag ctcgcccaca cgaaccatcc
     1801 gggcggccgc cacgccaagc gctggcagga cgtcgaggag ctgctcgcgg ccggcatcga
     1861 cgtgttcacg acggtgaaca tccagcacct cgagagcctg aacgacaagg tcctcgaggt
     1921 caccggggtg cgggtgcggg agaccttccc cgaccggctg ctccacgagg ccgacgaggt
     1981 cgttttggtg gacctgcccc ccgaagcgct gcgcgagcgg gtcatggtct gcatcgacgg
     2041 ccggcccgac tcgagcgggc tggtgcgccg ggccgcccgc atggcgcgcc ggtcgagcgg
     2101 gaggctctgg atgctccacg tcgagcccgc cgacgagcgc gagtcgtccc aggtccacga
     2161 gatggtggac gaggcggagg cgctcgccgg ccggctcggc gcgacggtgc tgcgccgcgc
     2221 gggcgacgtg gcggaggtcg tcgtcgaggc cgcccgcgag ctcgggatca cccagatcgt
     2281 gctcggcgag tcgcaccggc cgcgctggaa ggagctggtc ggccggtcga tcatcgcccg
     2341 catcctgcgg gagaccgacg gggtggacgt ccacatcatc gccgaccacc gggcacggcc
     2401 tccccgcccc accacccgca cagctgagga gggatagtcg atgccgcgca cgttcaccga
     2461 cgtccgaggc gacagcgcgg tctggggagg ggctgggttc gcggccagcg tactgctcgg
     2521 catcgccctc ggccccttcc gccacgacgt cggcctcgag aacgtggtca tcctctacct
     2581 ggccgtg
//
LOCUS       DASPYY010000785         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1115778, whole genome shotgun sequence.
ACCESSION   DASPYY010000785 DASPYY010000000
VERSION     DASPYY010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115778"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VEP73_12240"
     CDS             <1..336
                     /locus_tag="VEP73_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT27242.1"
                     /translation="FLGMVRQWQELFYEKRFSAVEFGYDVPDYVKLAEAYGGVGLRCD
                     QPEDVDATLEKAFAVEGVPTVVDFRVDSHEGVFPMVPAGRPNDEIILGPEFSPEEQAA
                     ATRREVPTA"
     gene            333..854
                     /gene="ilvN"
                     /locus_tag="VEP73_12245"
     CDS             333..854
                     /gene="ilvN"
                     /locus_tag="VEP73_12245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HYT27243.1"
                     /translation="MSFHTLSVLVENRPGVLARVAGLFSRRGFNIESLAVGPTEDESV
                     SRMTIVVDCAERPLEQVVKQLNKLISVLKIVELEPGRAVERELLLIKVKADPAVRYQI
                     LELAEVFRGKIVDVAPESLTIEATGSPDKVAAMLEMLEPYGVRELVRTGRVALARGNR
                     GITDRALRVPRSA"
     gene            901..1905
                     /gene="ilvC"
                     /locus_tag="VEP73_12250"
     CDS             901..1905
                     /gene="ilvC"
                     /locus_tag="VEP73_12250"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HYT27244.1"
                     /translation="MMATVYYDDDADLGLVQGRKVAVLGYGSQGHAHALSLRDSGCEV
                     RVGLGGGSASRPRAEAAGLAVSSPAEAVAWADLAMVLVPDTVQRRLYETDIAPNLKQG
                     DALFFAHGFNVHFGYITPPEGIDVAMVAPKAPGHLVRRQYEEGQGTPVLVAVAQDATG
                     KAWDLAKSYARAIGGTKAGALVTTFQEETETDLFGEQAVLCGGTSALVQAGFETLVKA
                     GYQPEVAYFECLHELKLIVDLLYEGGLSWMRYSVSDTAEYGDYTRGPRIVTDETRAEM
                     GRILAEVRSGEFAREWIAEDDQGRPNFARLREAARAHQIEQTGKPLRAMMSWLRRPGE
                     "
     gene            1915..3603
                     /gene="serA"
                     /locus_tag="VEP73_12255"
     CDS             1915..3603
                     /gene="serA"
                     /locus_tag="VEP73_12255"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYT27245.1"
                     /translation="MVRILIAEPLAEAGVELLRAHYEVDARAVMSRGELLREVVGVDA
                     LVVRSATRVDAEVLEAGRNLRVVGRAGIGLDNVDVDAATRLGVLVVNAPQSNVISAAE
                     HTVALVLAQARNIPQAHVRLREGHWDRSHFQGAELYGKTLGIVGLGRVGALVAQRLNA
                     FGMRLVAYDPYVSRDRAAQMGVELGSLSEVLARADVVTIHLPKTPETTGLIGERELAA
                     MRPGARLVNTARGGIVDEVALAKAVESGHLAGAALDVFAEEPTTQSPLFDLDRVVVTP
                     HLGASTAEAQDKAGVTIAEQLLLALSGEFVPNAVNVEAGPVPDALRPFLPLVEKLGRL
                     YTAFAAGGPDGRAVAVGGGRVTIEYVGKLASHDTRVLNLAALKGMFTPVVHEPVTYVN
                     APLLAAERGIEVAEMKTGQSRDWVNLVTLSGDGPRGPVSVAGTTVGPRDAERLVSING
                     IEVDMAPSEHMAFLVYEDRPGVIGRVGTLLGAAGVNIASMQVGRRKQGGEALMALTVD
                     AGIPAGVLDRVVEEIGAHEGTFIHLHSDPVRPGGPGEPDHGSPAGGPPADGAGA"
     gene            3737..>3892
                     /locus_tag="VEP73_12260"
     CDS             3737..>3892
                     /locus_tag="VEP73_12260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524016.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYT27246.1"
                     /translation="MAERITIFDTTLRDGEQSPGINLNAKAKLEIAEQLARLGVDVIE
                     AGFPVASQ"
BASE COUNT          556 a         1408 c         1417 g          511 t
ORIGIN      
        1 ttcctcggca tggtgcgcca gtggcaggag ctcttctacg agaagcgctt cagtgcggtg
       61 gagttcgggt acgacgtgcc cgactacgtg aagctcgccg aggcgtacgg cggcgtcggg
      121 ctgcgctgcg accagcccga ggacgtcgac gcgaccctgg agaaggcgtt cgcggtcgag
      181 ggggtgccga cggtggtgga cttccgggtc gacagtcacg agggcgtctt cccgatggtc
      241 ccggccggcc gtccgaacga cgagatcatc ctcggccccg agttctcgcc cgaggagcag
      301 gccgccgcga cccggcggga ggtgccgacc gcatgagctt ccacaccctt tccgtcctgg
      361 tcgagaaccg cccgggcgtg ctcgcccgcg tcgccgggct gttcagccgc cggggcttca
      421 acatcgagtc gctggccgtc ggccccaccg aggacgagtc ggtgagccgc atgacgatcg
      481 tggtcgactg tgccgagcgg ccgctcgagc aggtcgtcaa gcagctgaac aagctcatca
      541 gcgtgctcaa gatcgtcgag ctggagccgg ggcgcgcggt ggagcgcgag ctcctgctga
      601 tcaaggtcaa ggccgacccg gccgtccgct accagatcct cgagctcgcc gaggtgttcc
      661 ggggcaagat cgtcgacgtc gccccggagt cgctcaccat cgaggcgacc ggcagcccgg
      721 acaaggtcgc ggccatgctc gagatgctcg agccgtacgg cgtgcgcgag ctggtgcgca
      781 ccggccgggt cgcgctcgcc cggggcaacc ggggcatcac cgaccgcgcc ctccgcgtgc
      841 cacggtccgc ctgatggagg cgacccgcac gccccttcgt cacaggaacc cggaggttcg
      901 atgatggcaa ccgtctacta cgacgacgac gcagacctcg ggctcgtgca gggccgcaag
      961 gtcgccgtcc tcggctacgg ctcccagggc cacgcgcacg ccctctcgct gcgggactcc
     1021 ggctgcgagg tgcgggtcgg gctcggtggg ggctcggcgt cgcggccaag ggccgaggcg
     1081 gccgggctcg ccgtcagcag cccggccgag gcggtcgcct gggccgacct cgcgatggtg
     1141 ctcgtgcccg acacggtcca gcgccgcctc tacgagaccg acatcgcccc gaacctgaaa
     1201 cagggcgacg cgctgttctt cgcgcacggc ttcaacgtcc acttcggcta catcaccccg
     1261 cccgagggga tcgacgtcgc gatggtggcc cccaaggcgc ccgggcacct ggtccgccgc
     1321 cagtacgagg agggccaggg caccccggtg ctcgtggcgg tcgcccagga cgccaccggc
     1381 aaggcatggg acctggccaa gtcctacgcg cgggcgatcg gcggcaccaa ggcgggcgcg
     1441 ctcgtcacca cgttccagga ggagaccgag accgacctgt tcggcgagca ggccgtgctc
     1501 tgcggcggca cctccgcgct cgtccaggcc gggttcgaga cgctcgtcaa ggccggctac
     1561 cagcccgagg tggcctactt cgagtgcctg cacgagctga agctgatcgt cgacctgctc
     1621 tacgagggcg gcctgtcgtg gatgcggtac tcggtgtccg acacggccga gtacggcgac
     1681 tacacccgcg ggccgcggat cgtgaccgac gagaccaggg cggagatggg ccgcatcctc
     1741 gccgaggtcc ggtcgggcga gttcgcccgc gagtggatcg cagaggacga ccagggccgg
     1801 cccaacttcg cccgcctgcg cgaggccgcc cgcgcccacc agatcgagca gaccggcaag
     1861 ccgctccggg cgatgatgtc ctggctccgc cgccccggag agtagtctga cacgatggtc
     1921 cggatcctga tcgctgagcc gctggccgag gccggggtcg agctgctgcg cgcccactac
     1981 gaggtggacg cccgcgccgt gatgagccgc ggggagctgc tgcgcgaggt cgtcggggtc
     2041 gacgcgctcg tggtgcgctc ggcgacccgt gtcgacgccg aggtgctcga ggccgggagg
     2101 aacctcaggg tggtgggcag ggccgggatc ggcctggaca acgtcgacgt cgacgccgcc
     2161 acccgcctcg gggtgctcgt cgtcaacgcg ccgcagtcca acgtcatctc cgcggccgag
     2221 cacaccgtcg cgctggtcct cgcccaggca cgcaacatcc cccaggccca cgtccgcctc
     2281 cgcgagggcc actgggaccg ctcgcacttc cagggcgccg agctgtacgg caagacgctt
     2341 gggatcgtgg ggctcgggcg ggtcggcgcg ctggtcgccc agcgcctgaa cgccttcggc
     2401 atgcggctgg tcgcctacga cccgtacgtc tcccgcgacc gggccgccca gatgggggtg
     2461 gagctcggga gcctctccga ggtgctcgcc cgggccgacg tcgtgaccat ccacctgccg
     2521 aagaccccgg agaccaccgg gctcatcggc gagcgggagc tggcggccat gcgcccgggg
     2581 gcgcgcctcg tcaacaccgc gcggggcggc atcgtcgacg aggtcgcgct cgccaaggcg
     2641 gtcgagtccg gccacctcgc cggcgccgcc ctcgacgtgt tcgccgagga gccgaccacg
     2701 cagagcccgc tgttcgacct cgaccgcgtc gtcgtcacgc cgcacctcgg cgcgtcgacc
     2761 gccgaggccc aggacaaggc cggggtgacg atcgccgagc agctcctgct cgcgctctcc
     2821 ggcgagttcg tccccaacgc ggtcaacgtg gaggccggtc cggtgcccga cgcgctgcgc
     2881 ccgttcctcc cgctggtgga gaagctgggc cggctctaca cggcgttcgc cgccggcggc
     2941 ccggacggcc gggcggtcgc cgtgggcggt gggcgcgtga cgatcgagta cgtcggcaag
     3001 ctcgcctcgc acgacacccg cgtgctgaac ctcgccgcgc tcaagggcat gttcaccccg
     3061 gtcgtccacg agccggtgac ctacgtgaac gccccgctgc tcgcggccga gcgcggcatc
     3121 gaggtggccg agatgaagac gggccagagc cgcgactggg tcaacctcgt cacgctgtcc
     3181 ggggacggcc cgcggggacc ggtgagcgtc gccgggacca ccgtcggccc gcgcgacgcc
     3241 gagcgcctgg tctcgatcaa cggcatcgag gtcgacatgg cgccgagcga gcacatggcg
     3301 ttcctcgtct acgaggaccg gcccggcgtc atcggccggg tcggcacgct gctcggcgcg
     3361 gccggcgtga acatcgccag catgcaggtc ggccggcgca agcagggcgg cgaggccctg
     3421 atggcgctga ccgtggacgc gggcatcccc gccggcgtcc tcgaccgggt ggtggaggag
     3481 atcggcgccc acgaaggcac cttcatccac ctccactcgg acccggtgcg gcccggcggc
     3541 cccggcgagc ccgaccacgg ctcgccggcc ggcggaccgc ccgccgacgg agcaggggcg
     3601 tgatccggac gacctccgtg acgccgagcc tccgcatcct tcgctgccgt ggggcggcct
     3661 cgtgccggct gcctcacgga gagcctcacg gcgcgacagg agacggaagc ggagcacgca
     3721 ctggagggat tcggctgtgg ccgagcggat cacgatcttc gacacgaccc tgagggacgg
     3781 cgagcagtcc cccggcatca acctgaacgc gaaggccaag ctcgagatcg ccgagcagct
     3841 ggcccggctg ggcgtcgacg tcatcgaggc ggggttcccg gtggcgagcc ag
//
LOCUS       DASPYY010000786         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1539618, whole genome shotgun sequence.
ACCESSION   DASPYY010000786 DASPYY010000000
VERSION     DASPYY010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539618"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEP73_12265"
     CDS             complement(<1..186)
                     /locus_tag="VEP73_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27247.1"
                     /translation="MTDRLKVDRDLGVCASSGFSAAAVACGIKQSGGPDLALVVGVPG
                     TLAPGAGQAELSACLADA"
     gene            complement(203..1255)
                     /gene="argC"
                     /locus_tag="VEP73_12270"
     CDS             complement(203..1255)
                     /gene="argC"
                     /locus_tag="VEP73_12270"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HYT27248.1"
                     /translation="MTMTVGILGASGYAGAELLRLLARHPHLEVAWAAGDRTAGQLLA
                     ERYPGLADAYGDLAFCTADEGLDKGADALFLALPHGDSAGGLAARATLAAGLAVDLSA
                     DFRLHDARAYPVWYGAPHPCPEELGAWPYGLPELHRDELAGAAKIAVGGCYPTAALLA
                     LAPLLAAGLVEPDGIVVDAKSGLTGAGRSLKDPYLYAQANENVTPYGIGSHRHTPEIE
                     QELSRLAGRPLRVSFTPHLVPQSRGLLATCYATFADPDLAAPRGVASCYQAAYRDEPF
                     IDLLGEGRWPGTRAVATTNRVQVAVTADPRTNRLVVAAAIDNLVKGAAGQAVQCANLA
                     LGLPETAGLDLVPPGP"
     gene            1475..2812
                     /gene="lysA"
                     /locus_tag="VEP73_12275"
     CDS             1475..2812
                     /gene="lysA"
                     /locus_tag="VEP73_12275"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYT27249.1"
                     /translation="MNETTTVDGTTTADGTADGTAADGGTGHPFRYAAGVLHVGGLPL
                     DRVVAATGTPAYVYDLDAVAAAYRRIADAFAPLGGRVLYAVKANSSLAVLACLARLGA
                     GFDVVGGGELVRVLEAGGDPGQVVFAGVGKTPAELALAVGHDVVVHVESADELDALEM
                     AAARAGRTARFALRVNPDVEVDTHAYIQTGHDEAKFGVPAEVAEELFGRTAKGDYPHL
                     HPVGVHVHVGSQLPDPDGLVDAARTGLGMIESGRAAGLELHALDVGGGLPISYDGDNR
                     GAMLPGPEVFAQALAPVVAGREVTLVIEPGRYLVGAAGALVASVLYRKHRSAGRMLVV
                     DTGMHHLIRPALYQATHRVVPLRQAPSAGPTEVVGPICESTDVLAPAADLPDLAPGDL
                     LAFLDTGAYGMTMASNYNSHPRPPEIVVEGGTARVTRRRETWDDLLAFEDSES"
     gene            complement(2809..3012)
                     /locus_tag="VEP73_12280"
     CDS             complement(2809..3012)
                     /locus_tag="VEP73_12280"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HYT27250.1"
                     /translation="MVNVGDRVQIESEKVGSVTRIGTVTGIEGRMITVRWDSGSQSMF
                     VPSAGCLQVIGHEGVAQPQVERS"
     gene            3202..>3774
                     /locus_tag="VEP73_12285"
     CDS             3202..>3774
                     /locus_tag="VEP73_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27251.1"
                     /translation="MADVLLEDLSRVHDDGTVAVDHLNLHVRDHELLALLGPSGCGKS
                     TTLRLIAGVEPLTSGTITIGGRVVSNLPPHERDVAMVFETNSVYPHLTGGENLRFSLA
                     LRRLPKEEIDQRVQAEARVLRIRHLLARLPKAMSAGQRQRVGLGRATVRVPTVYLLDE
                     PLTHLDEAERVRLRTELNALLHGLGVTTIYV"
BASE COUNT          546 a         1408 c         1342 g          478 t
ORIGIN      
        1 ggcgtcggcc aggcaggcgg acagctccgc ctggcccgcg ccaggcgcga gcgtgcccgg
       61 caccccgacg accagggcga ggtcggggcc gccggactgc ttgatgccgc aggcgactgc
      121 ggcggcgctg aacccgctgg aggcacagac accaagatcg cggtcaacct tgagccgatc
      181 ggtcatcggg tacatcaccg ccttacggtc ccggtgggac caggtcgagg ccagccgtct
      241 ccggcaggcc gagggcgagg ttggcgcact gcaccgcctg gccggcggcg cccttgacga
      301 ggttgtcgat cgcggcggcg acgaccagcc ggttggtgcg cgggtcggcg gtgaccgcca
      361 cctggacccg gttggtggtg gcgaccgccc gggtcccagg ccagcggccc tcgccgagca
      421 ggtcgatgaa gggctcgtcc cggtaggcgg cctggtagca ggaggccacc ccgcgcggcg
      481 cggccaggtc cgggtccgcg aaggtggcgt agcaggtggc gagcaatccc cggctctgcg
      541 gcaccagatg gggcgtgaag ctgacccgca gcggccgccc ggcgagacgg ctcagctcct
      601 gctcgatctc gggggtatgg cgatggctgc cgatgccgta gggggtgacg ttctcgttgg
      661 cctgcgcgta gaggtatggg tccttgagcg agcggccggc cccggtgagc cccgacttcg
      721 cgtcgacgac gatgccgtcc ggctcgacca ggccggcggc caggagcggg gccagggcga
      781 gcagcgccgc cgtcgggtag cagccgccga cggcgatctt ggccgccccg gcgagctcgt
      841 cgcggtgcag ctcgggcagg ccgtagggcc aggcgccgag ctcctccggg caggggtggg
      901 gcgcgccgta ccagaccggg tacgctcgtg cgtcgtggag ccggaagtcg gccgagagat
      961 cgacggccag gccggccgcc agggtggccc tggcggcgag cccgccggcg ctgtcgccgt
     1021 ggggcagcgc caggaacagc gcgtcggcgc ccttgtcgag gccctcgtcg gcggtgcaga
     1081 aggcgaggtc gccgtaggcg tcggcaaggc cggggtaccg ctcggccagc agctggccgg
     1141 cggtgcggtc gcccgcggcc caggcgacct ccaggtgcgg gtggcgggcg agcaggcgca
     1201 gcagctcggc cccggcgtag ccggaggcgc ccaggatccc gacggtcatc gtcatggcgc
     1261 atgagtatgc aggttccggt gaaaatatgc aatggatgtc cggtgcggcg cggcgcacgc
     1321 cgaattctgg ttgcacgcgg ctcggagcgt atgcaaagca tcacccacgg tggcagccgg
     1381 cggcatcatg ggtgctgcga gccgaccggc cgaacgccgg cagcggcacg ctggcaacgg
     1441 cgccaccgga ccgagcagcg ggggaccctg cgccatgaac gagacgacga cggtggacgg
     1501 cacgacgacg gcggacggca cggcggacgg cacggcggcg gacggcggca ccgggcaccc
     1561 cttccggtac gcggcgggcg tgctccacgt cggcgggctg ccgctcgacc gggtggtggc
     1621 cgcgacaggc acccccgcct acgtctacga cctggacgcc gtggcggccg cctaccggcg
     1681 gatcgccgac gccttcgcgc cgctcggcgg ccgcgtgctg tacgcggtca aggccaactc
     1741 cagcctcgcc gtcctcgcct gcctggcccg gctcggcgcc ggcttcgacg tcgtcggggg
     1801 cggcgagctg gtgcgcgtgc tcgaggccgg cggcgacccg ggccaggtgg tgttcgcggg
     1861 ggtcggcaag acgccggccg agctcgctct ggccgtcggg cacgacgtcg tcgtccacgt
     1921 cgagtcggcc gacgagctgg acgcactgga gatggccgcc gcccgcgccg gccgcaccgc
     1981 ccgcttcgcg ctgcgcgtca accccgacgt cgaagtcgac acccacgcct acatccagac
     2041 cggccacgac gaggccaagt tcggcgtccc cgccgaggtc gccgaggaac tcttcggccg
     2101 caccgccaaa ggcgactacc cgcacctgca ccccgtcggc gtgcacgtcc acgtcggctc
     2161 gcaactgccc gacccggacg gcctggtcga cgccgcccgc accggcctgg ggatgatcga
     2221 gtcaggccgc gccgccggcc tggaactgca tgccctcgac gtcggcggcg gcctcccgat
     2281 cagctacgac ggcgacaacc ggggcgccat gctgcccggc cccgaggtgt tcgcccaggc
     2341 cctcgccccg gtggtggccg gccgcgaggt gaccctggtg atcgagccgg gccgctacct
     2401 ggtcggcgcc gccggcgccc tggtcgccag cgtcctgtac cgcaagcacc gcagcgccgg
     2461 ccgcatgctg gtggtcgaca ctggtatgca ccacctcatt cgcccggccc tctaccaggc
     2521 aacccaccgg gtcgtcccgc tccgccaggc cccctccgcc ggccccaccg aggtcgtcgg
     2581 cccgatctgc gagtccaccg acgtcctcgc ccccgccgcc gacctccccg acctggcccc
     2641 cggcgacctg ctcgccttcc tcgacaccgg cgcctacggc atgaccatgg cctccaacta
     2701 caacagccac cccaggcccc ccgaaatcgt ggtcgaaggc ggcacggcca gggtcacccg
     2761 ccgccgcgag acctgggacg acctgctggc cttcgaggac tcggagagct agctccgctc
     2821 aacctgcggt tgcgccaccc cctcgtggcc gatgacctgc aggcaaccgg cgctcggcac
     2881 aaacatcgac tggctgcccg agtcccagcg aacggtgatc atgcggccct cgatcccggt
     2941 caccgtgccg atccgggtca cgctgcccac cttctcggac tcgatctgga cacgatcccc
     3001 gacgttgacc atcgctgacc cccagcactc gaatcagtca ggaagcggga gaaatacgac
     3061 gatcgagtgt gacgccctaa ccgcaaaccg gcaagagccc agtccaagtc cttctctgtc
     3121 cttctctggt catgaacctc ggccacgcct accatcgagg cggagagcaa gcgggctgag
     3181 tgcccggatc gaggagccgg catggcggac gtgctgctcg aggacctctc acgggtccac
     3241 gacgacggca ccgtcgccgt cgaccacctc aacctccacg tgcgcgacca cgagctgctg
     3301 gcgctgctgg gcccgtcggg gtgcggcaag agcaccaccc tgcggctgat cgccggggtc
     3361 gagccgctca ctagcgggac catcaccatc ggcggacggg tcgtcagcaa cctgccgccg
     3421 cacgaacgcg atgtggccat ggtcttcgag accaactcgg tatacccgca cctgaccggc
     3481 ggcgagaacc tacgcttcag cctcgccctg cggcgcctgc caaaagagga gatcgaccag
     3541 cgggtccagg ccgaggcgcg ggtgctgcgc atccgccacc tgctggcgcg gctgccaaag
     3601 gccatgtccg ccggccagcg ccagcgggtc gggctgggcc gcgccaccgt gcgggtcccc
     3661 accgtctacc tgctggacga gccgctcacc cacctcgacg aagccgagcg ggtgcggctg
     3721 cgcaccgaac tgaacgctct cctccacggc ctcggggtga cgaccatcta cgtc
//
LOCUS       DASPYY010000787         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_411795, whole genome shotgun sequence.
ACCESSION   DASPYY010000787 DASPYY010000000
VERSION     DASPYY010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411795"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VEP73_12290"
     CDS             complement(<1..1036)
                     /locus_tag="VEP73_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27252.1"
                     /translation="MTAAGRWLARRTDEQVFGACLAGIAAAVGLFLVVPLTPQFLAIH
                     RVPTNLDYNVIGKPEGRVTAFWALAMASFGCAVWQWRRGRRPSVRLLAAGVALLTLLA
                     LLVPPVASEDVYAYSFYGRVQSFYHVNPYVSFPEQYSLDPWYHLWSWRLYGPVYGPPF
                     LLLLRVVAAAAGQSLLAFVVMMKLLLVAAELLAVWMLARALRRRRPGAGGAAGAAGTT
                     DARWPVLLIAWNPMVLQAVAMSAHVDALLLLAVAGAVLAHARGRHLAAFLLLVLTFLV
                     KVYMGPLAALYGLWLAFARVPGRPLGRRLAGLAGLGGLGAVATALAYLPYAGAGTRLF
                     ASAVDVSDHFS"
     gene            complement(1033..>1660)
                     /locus_tag="VEP73_12295"
     CDS             complement(1033..>1660)
                     /locus_tag="VEP73_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HYT27253.1"
                     /translation="AFSRLAGELRACGLVAGTVTTGQAFGGDLEAISLYSGLVAARAV
                     LRADVVVVAQGPGGMGSGTRFGFSGTQTAEAVNAVAALGGRPVACLRMSTADPRPRHR
                     GVSHHSLTALGRLALGRAAVAVPALDDADLAATVDGQLHEAGVADRHELVRVAAPDAA
                     ELLAGWGLRVTTMGRGPGDDPVFFAAAAAAGTLAGRLAAGPGGQGRAP"
BASE COUNT          245 a          617 c          656 g          142 t
ORIGIN      
        1 tcgagaagtg gtcgctgacg tccaccgcgc tcgcgaacag ccgcgtgccg gcgcccgcgt
       61 acggcaggta cgcgagcgcg gtggccacgg cgccgaggcc gccgaggccg gccaggccgg
      121 cgagcctgcg cccgaggggc cgcccaggca cccgcgcgaa cgccagccac agcccgtaga
      181 gggcggcgag cggccccatg tagaccttga ccaggaacgt gaggacgagc agcaggaacg
      241 cggcgaggtg gcggccccgc gcgtgcgcca ggacggcgcc ggccacggcc aggagcagca
      301 gggcgtcgac gtgggccgac atggcgaccg cctggagcac catcgggttc caggcgatga
      361 gcagcaccgg ccagcgcgcg tcggtcgttc ccgccgcgcc cgccgcgccg ccggcacccg
      421 gccggcggcg gcggagcgcg cgggccagca tccagaccgc gaggagctcg gcggcgacga
      481 gcagcagctt catcatgacg acgaaggcga gcaggctctg gccggcggcg gccgcgacca
      541 cgcggagcag gagcaggaac ggcggcccgt acaccgggcc gtacaggcgc cacgaccaca
      601 ggtggtacca ggggtccagg ctgtactgct ccgggaacga cacgtacggg ttgacgtggt
      661 agaagctctg gacccggccg tagaacgagt aggcgtagac gtcctcggag gcgaccggcg
      721 gcaccagcag ggcgagcagg gtgagcaggg cgacaccggc ggccagcagc cgcacgctcg
      781 gcctgcggcc ccggcgccac tgccacacgg cgcagccgaa cgaggccatg gcgagcgccc
      841 agaaggcggt gacccggccc tcgggcttgc cgatcacgtt gtagtccagg ttcgtcggca
      901 cccggtggat ggcgaggaac tgcggggtga gggggacgac caggaacagc cccacggcag
      961 cggcgatgcc ggcgaggcag gccccgaaca cctgctcgtc ggtccgcctg gcgagccagc
     1021 ggccggccgc ggtcacggcg ccctcccctg gccgcccggt ccggcggcca ggcggccggc
     1081 cagcgtcccg gcggccgcgg cggcggcgaa gaagaccggg tcgtcgccgg ggccgcggcc
     1141 catggtggtg acccgcaggc cccacccggc gagcagctcg gccgcgtcgg gcgcggcgac
     1201 gcggaccagc tcatgacggt cggccacccc ggcctcgtgg agctgcccgt cgaccgtggc
     1261 ggccaggtcg gcgtcgtcga gggcgggcac ggccaccgcg gcccgcccga gggcgagccg
     1321 cccgagcgcg gtcaggctgt gatggctcac cccccggtgc cgcggccggg ggtcggcggt
     1381 cgacatgcgc aggcaggcca ccggccggcc gccgagggcg gcgacggcgt tgaccgcctc
     1441 ggccgtctgc gtgccggaga acccgaacct ggtgcccgag cccatcccgc ccgggccctg
     1501 ggcgacgacc acgacgtcgg cccgcagcac ggccctggcg gcgaccaggc ccgagtacag
     1561 gctgatcgcc tcgaggtcgc cgccgaacgc ctggccggtg gtgacggtgc ctgccacgag
     1621 cccgcaggca cggagctcgc ccgcgagccg ggagaacgcc
//
LOCUS       DASPYY010000788         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1116488, whole genome shotgun sequence.
ACCESSION   DASPYY010000788 DASPYY010000000
VERSION     DASPYY010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116488"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEP73_12300"
     CDS             complement(<1..277)
                     /locus_tag="VEP73_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27254.1"
                     /translation="MTVAVRAEGLAKTFGATRALDGVDLEVEGGTVLGLLGPNGAGKT
                     TTVRILATLLRPTAGRAEIGGYDVVRQPQQVRQLIGLTGQYAAVDENL"
     gene            complement(589..711)
                     /locus_tag="VEP73_12305"
                     /pseudo
     CDS             complement(589..711)
                     /locus_tag="VEP73_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
     gene            831..1721
                     /locus_tag="VEP73_12310"
     CDS             831..1721
                     /locus_tag="VEP73_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HYT27255.1"
                     /translation="MTSNRTIETYLMDMDGVLVHQEQLIPGADQFVKRLEETGHRFLV
                     LTNNSIYTPRDLAARLRLTGLEVPEEAIWTSALATARFLDQQRPGGTAYVIGEAGLTT
                     ALHERGYVLSEREPDYVVLGETRSYSLDAVTRAIRLIAAGARFIATNPDPTGPSPAGP
                     QPATGAVAALISKATGVDPYFVGKPNPLMMREALRAIDAHSESTVMIGDRMDTDIVAG
                     MEAGLATVLVLSGISTREQAGRFPFLPSRIVDSVADLVGELAQQPTPGPAAGWPPAGR
                     RSARRRALEVAGHDLPSDRA"
     gene            1975..>3181
                     /locus_tag="VEP73_12315"
     CDS             1975..>3181
                     /locus_tag="VEP73_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYT27256.1"
                     /translation="MGWENPRPRLAPRGARAPRGVRYAELHCHSNFSFLDGASHPEEL
                     AEEAARLGLEALALTDHDGMYGAVRFAEAARAVGLPTVLGAELTLGLPPDAGGRGHRN
                     RPDPAGEHLVVLARGPAGYARLCRAVSAAQLAGSKGAPRIDLARLAGLHGGHWLVLTG
                     CRKGAVPRALAEHGPAAASWQLDRLVGAFGRENLAVEVWDHGDPLDSARNDALVRLAD
                     LAGVEVVATNDVHYAVPARRRLATALAAVRARRPLDELDGWLPAAATAHLRSGLEQAT
                     RLSRYPGVVERAADLGRALAFDLELVAPRLPDFPVPDGHTEMSWLRELAEQGAARRYG
                     PRDAERVPGAWRQIDHELAMVEQLGFPGYFLTVWDIVRFCEREGIYCQGRGSAANSAV
                     CYALGITKAD"
BASE COUNT          417 a         1202 c         1138 g          424 t
ORIGIN      
        1 tcaggttctc gtcgacggcg gcgtactggc cggtcaggcc gatgagctgg cggacctgtt
       61 gtggctggcg gacgacgtcg tagccgccga tctcggcgcg gccggcggtc gggcgcagca
      121 gcgtggccag gatccggacg gtggtggtct tgcccgcgcc gttggggccg agcagcccga
      181 gcacggtgcc gccctcgacc tcgaggtcga ccccgtcgag cgcccgggtg gccccgaagg
      241 tcttggccag gccctcggcc cggaccgcga cagtcatgac agcctccctc cgcgacctgc
      301 agcgggtgga actcgagcac gttggtgccg accgcgacga ccccggcgtc gccctggtcg
      361 ctgaggggga cggatccgcg cagctcggtg gcgtagcaac cgaggtccct gatgggcggc
      421 ccatgcggga agtaggtgcc ggaccttgcc aggtacgagc cgaccgtcgt gacggaacgc
      481 gccggctacg ccggcggctg ggcggcccat catcgcattg tcgaccggca ccacttcgag
      541 gtcaacgaaa cacgaccacg gttcgctgcg ttccgaggtc gagcgccccg tcctgtcctg
      601 gcggagcgtt ccgttcaccc acagccgcag ccgcagtccg gccagccgag ggaattcctc
      661 cggctcgtgc agaaccaggc ggggaccggt cggggtgaaa agtgaacgag ctgcaccagc
      721 tcggcgagag ttcatctgat gctctcgggg gcgttacacg cccgtgaccg tcggccggca
      781 ccctgtgaag tgattcacaa gaccccgagt gggacggagc aagtgcaacc atgaccagca
      841 accgcacgat cgagacctac ctgatggaca tggacggcgt cctcgtccac caggagcagc
      901 tgatccccgg tgccgaccag ttcgtcaagc gcctcgagga gaccgggcac cggttcctcg
      961 tcctgaccaa caactccatc tacacccccc gcgacctggc cgcccggctc cgcctgaccg
     1021 gcctcgaggt ccccgaggag gcgatctgga cctcggcgct ggccaccgcc cggttcctgg
     1081 accagcaacg ccccggcggg accgcgtacg tgatcggcga ggccgggctg accaccgcgc
     1141 tgcacgagcg tggctacgtg ctgagcgagc gcgagcccga ctacgtggtc ctgggcgaga
     1201 cccgcagcta cagcctggac gcggtcaccc gcgcgatccg gctgatcgcc gccggcgccc
     1261 gcttcatcgc caccaacccc gacccgaccg gcccctcgcc cgccggcccg cagccggcca
     1321 ccggcgcggt cgcggcactg atctccaagg cgaccggggt cgacccgtac ttcgtcggca
     1381 agccgaaccc gctgatgatg cgcgaggccc tgcgggccat cgacgcgcac tcggagtcca
     1441 cggtgatgat cggcgaccgg atggacaccg acatcgtggc cggcatggag gcgggcctcg
     1501 ccacggtgct ggtgctgtcg ggcatctcca cccgcgagca ggccgggcgg ttcccgttcc
     1561 tgccctcccg gatcgtggac tcggtcgccg acctggtcgg cgagctcgcc cagcagccca
     1621 cgccagggcc ggcggcgggc tggccgccgg ccggtcggcg ctcggcccgc cggcgggcgc
     1681 tggaggtggc cggccacgac cttccgtccg accgtgcttg aggccgaccc tggccgccgg
     1741 ctgcgctggc ccggcgggct ggcgtggccg gcccatatcg cggtcgaccg cggcgtcgac
     1801 cccctgcgag cgtcgatcgg gccggtcgcg acagtggggc caccagtggt gtctgcctgc
     1861 cgcattggta aatgggatgg tccgagagga attctatgag cttgcgttct gcgtgtcgct
     1921 cgtggattgg gggcaggttg caataggcga acgtgtgttc gatcatctcg gaatatggga
     1981 tgggaaaacc cacggccgcg cctggctccg cggggggccc gggccccccg cggggtccgc
     2041 tacgccgagc tgcactgcca ctccaacttc agcttcctcg acggcgcgtc ccacccggag
     2101 gagctggccg aggaggcggc ccggctcggc ctcgaggcgc tcgccctcac cgaccacgac
     2161 ggcatgtacg gggcggtccg cttcgccgag gcggccaggg cggtcggcct gcccaccgtg
     2221 ctcggcgccg agctgaccct gggcctcccc ccggacgccg gggggcgggg ccaccgcaac
     2281 cgccccgacc cggccgggga gcacctcgtc gtgctggccc ggggccccgc cggctacgcc
     2341 cggctctgcc gagccgtctc ggcggcccag ctggcggggt ccaagggcgc cccccgcatc
     2401 gacctcgccc ggctcgccgg cctccacggc ggccactggc tggtcctcac cggctgccgc
     2461 aagggggcgg tgccgcgggc gctggccgag cacgggccgg ccgccgcctc ctggcagctc
     2521 gaccgcctgg tcggcgcctt cggccgcgag aacctcgccg tcgaggtgtg ggaccacggc
     2581 gacccgctcg actcggcccg caacgacgcc ctggtacggc tcgccgacct cgccggcgtg
     2641 gaagtggtgg cgacgaacga cgtccactac gccgtcccgg cccggcgccg gctggccacc
     2701 gccctggccg cggtgcgggc caggcgcccg ctcgacgagc tggacggctg gctgccggcc
     2761 gccgcgaccg cccacctccg ctccgggctc gagcaggcca cccgcctcag ccgctacccg
     2821 ggcgtggtcg agcgcgccgc ggacctcggc cgcgcgctcg ccttcgacct cgagctggtc
     2881 gccccgcggc tgcccgactt cccggtcccg gacgggcaca ccgagatgag ctggctgcgc
     2941 gagctggccg agcagggggc ggcccggcgc tacggccccc gggacgccga gcgggtgcct
     3001 ggcgcctggc gccagatcga ccacgagctg gccatggtcg agcagctcgg cttccccggc
     3061 tacttcctga ccgtctggga catcgtgcgg ttctgcgagc gcgagggcat ctactgccag
     3121 ggaaggggct cggcggccaa ctcggccgtc tgctacgcgc tcggcatcac caaggccgac
     3181 g
//
LOCUS       DASPYY010000789         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2661028, whole genome shotgun sequence.
ACCESSION   DASPYY010000789 DASPYY010000000
VERSION     DASPYY010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661028"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VEP73_12320"
     CDS             complement(<1..510)
                     /locus_tag="VEP73_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27257.1"
                     /translation="MAVTLAARLARPRWQPGVAAWTLWALVMLGVPVAVWSNHLLRQA
                     GRSDQAGSDVDTIIYLLAAVSAVTVGTELASRRPRHPVGWLLLALGLWFTFGYASWAL
                     LAWRGAPPAVGSAAKDVSVTRSSCWISRFGASRSSSMGLALAYNAVPRQPVTAGTTTW
                     PTMAWLPPPV"
     gene            738..1538
                     /locus_tag="VEP73_12325"
     CDS             738..1538
                     /locus_tag="VEP73_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT27258.1"
                     /translation="MAKPAELWARGGAYEPYVGRWSRLVAREFLAWLAVGEGAGWLDV
                     GCGTGALTQTTLTVADPAAVLGVDPSAGYVAYARTQSTDRRARFAVADARRLPVADDR
                     MDAVVSGLVLNFVPDPGQAVREMVRVTRAGGRVAAYVWDYAGRMELIRCFWDAAAVVD
                     PAAVELDEGRRFGLCRPEPLARVFQDAGLVEVRTRPIDLATRFRDFADYWTPFLGGEG
                     PAPGYAMSLQAEQRTALREELRARLPVAPDGSISLVVRAWAVQGACGR"
     gene            complement(1641..2603)
                     /locus_tag="VEP73_12330"
     CDS             complement(1641..2603)
                     /locus_tag="VEP73_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HYT27259.1"
                     /translation="MEQHEREHGAGGAGPPGRRRPPPPAARLRVLVIGAGLMGSQIGC
                     EYARAGHAVAFAARDPARARSAVGRVLAEARDLGVFSPGEAEAAAGRVTVGDLTAAGG
                     EPADLVVESVPEDLELKASVLRGPAAAMPEAVLATNTSSLSVTALGDRIGAPERTVGT
                     HYWNPPLLMPLVELVAGERTDPAVLERTRRALESAGKRPVEVRKEVPGFLWNRLQTAL
                     LREALWLVEQGVATPETVDEVIRDGLARRSRLTGPFETAALGGVDTFMRVAANLLPQL
                     SSATDAAGLARWTGRSPAELEALRRRRDRALAAELARERRDVLA"
     gene            2845..4506
                     /locus_tag="VEP73_12335"
     CDS             2845..4506
                     /locus_tag="VEP73_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT27260.1"
                     /translation="MRFQEPFFQEPPGLRNTYRSDVVLREYLERLLPAEIRAQVEPEL
                     DQMGEAAAGELALLAEEAEGSPPRLVQYDPWGRRIDRIELSPAWTELHAVQARAGLCA
                     LPYEDRHGRYDRIVQHALIHLYAPSSAVCTCPVAMTDAAVRVLVDHAPAELRERVVPR
                     LTSRDPERAWTSGQWMTEREGGSDVGRTSTVARCHEAGDWRLYGVKWFTSATTADCAL
                     ALARPDGAPEGSRGLGLFLVERVDPRSGRTQLGSTILVNRLKDKLGTRALPTAELTLA
                     GAHATPVGSVDRGLAKIAGMLNVTRLHNGLSSAAGMRRGVELAVAYARVRQAFGRPLI
                     DLPLHRATLADLAVEAEAGFALVSRGMELMGRVEHGLASEPEQRALRALVPTVKLLTA
                     KDAVAHASEVLEAFGGAGYVESTGVPRLLRDAQVLPIWEGTTNLLSLDLLRAESREEA
                     VTALLGDLSEQIAAVRAAPETGVSVDLVERSHAALLERVRAWPDADGDALQEQMRDFA
                     LRLGRTCAAALLAHHAAHRRAHRGDDRAAQVAHCYAERRLRAAVA"
     gene            4529..5527
                     /locus_tag="VEP73_12340"
     CDS             4529..5527
                     /locus_tag="VEP73_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYT27261.1"
                     /translation="METFKALVAERHDDRVDRRTDSLTLEDLPQGEVTVRVAWSSVNY
                     KDALAVSPKGRVARISPLVPGIDLAGEVTASDAPEFRPGDRVLAHGYDLGVSHFGGFA
                     EYARVPAGWVVPLPAGLSLRQAMALGTAGYTAGLSVQSLEERGLHPGAGPVLVLGASG
                     GVGSTAVGILAGRGYEVWASTGKREEEAFLRGLGASEVLPREETSAEGSRPLEPARWA
                     GCVDPVGGASLAYALRTLREGAAVAASGNTGGVELHTTVLPFILRGVALLGVDSAHTP
                     IERRRRVWERLAGDLRPRGLDEWITREVSLDDLGGVLDDTLAGRGRGRTVVRVGGG"
     gene            5698..>5914
                     /locus_tag="VEP73_12345"
     CDS             5698..>5914
                     /locus_tag="VEP73_12345"
                     /inference="COORDINATES: protein motif:HMM:NF022388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2637 domain-containing protein"
                     /protein_id="HYT27262.1"
                     /translation="MSVGPLPPTSAAADQWLLRFRSILVAILGTMVAGIACLAFYTSF
                     EAIRAYAMRSAGIAPEHGWAIPLLVDSF"
BASE COUNT          751 a         2164 c         2237 g          762 t
ORIGIN      
        1 caccggcggc ggcagccacg ccatagtcgg ccaggtggtc gtccccgccg tcaccggctg
       61 acgcggcacc gcgttgtagg ccaaggccaa ccccatgctc gacgacctcg acgccccgaa
      121 tctcgagatc cagcagctgg acctcgtgac cgagacgtcc ttggccgcag agccgacggc
      181 gggcggcgcc ccacgccagg ccaacagcgc ccagcttgca tacccgaagg tgaaccacag
      241 gccaagtgcc agcagcagcc agcccaccgg atggcggggc cggcggctgg ccagctccgt
      301 cccgaccgtc accgcgctca ccgccgccag caggtagatg atggtgtcga cgtccgaccc
      361 tgcctggtcg gaccggccgg cctggcgcag caggtggttc gaccagacgg ccacggggac
      421 gccgagcatg accagcgccc acagcgtcca agccgccacg cccggctgcc agcgcggccg
      481 agccagtcgg gcggccaggg tcaccgccat ggtcctggct gcacgcagcg caccaccgcc
      541 accctccctg cctcgcggtc acgtgccagt gtggccggcc tccgtctcgg tagccttacg
      601 gacgacagac ctgcccaacg cacatcaggc cttgtcgatg agcgttggat caggaatgag
      661 cagttgtctg gtggctggac cctcgcgccg gcgaacctca gacttggtcg gtccatatcg
      721 acagtgagga gccgacgatg gccaagccgg cagagctgtg ggcaaggggc ggcgcctatg
      781 agccgtatgt cggccgttgg agccggctgg tagcccggga gttcctcgcc tggctcgcgg
      841 ttggcgaggg cgctgggtgg ctggatgtgg gctgcgggac cggcgcgctc acccagacga
      901 cactgacggt ggccgatccg gccgcggtgt tgggggttga cccgtcggcg ggctatgtcg
      961 cctacgcgcg cacgcagagc accgaccggc gtgcccggtt cgcggtcgcc gatgcccgca
     1021 ggttgccggt cgcggatgac cggatggacg ccgtcgtgag cgggctggtg ttgaacttcg
     1081 tacccgaccc tggccaggcg gtgcgcgaga tggtccgggt gacccgggcg ggcggtcggg
     1141 ttgccgccta cgtctgggac tacgccggca ggatggagct gatccgctgc ttctgggatg
     1201 ccgcagccgt cgtggacccg gccgcggtcg agctggacga gggccggcgg ttcgggctgt
     1261 gccgaccgga gccgctcgcg cgcgtattcc aggacgccgg gctggtggag gttcggaccc
     1321 gcccgatcga tctcgccacc aggtttcgcg actttgccga ctactggacg ccgtttcttg
     1381 gaggcgaggg accagccccg ggctatgcga tgtcgctgca ggccgagcag cgaacagcgc
     1441 tgcgggagga gctgcgcgcg cggctgccgg ttgccccgga cggctccatc tcgctggtcg
     1501 ttcgggcctg ggcggtgcaa ggagcatgcg ggcgctagga acgacctgtc gagcggccag
     1561 acggctccct gcggcatcgc ggagcgacgg gctcgcgccg cgatgaccgc caagctcaag
     1621 agcgaggacg gcaaggcccg ctacgccaga acgtcccgcc gctcgcgggc gagctcggcg
     1681 gccagggcgc ggtcgcggcg gcgtcggagc gcctccagct cggcggggga gcggccggtc
     1741 cagcgcgcga gcccggcggc gtcggtcgcg ctcgagagct gcggcaacag gttggcggcg
     1801 acgcgcatga acgtgtcgac gcccccgagc gcagcggtct cgaacggccc ggtcagccgc
     1861 gatcggcgcg cgagcccgtc cctgatcacc tcgtcgacgg tctcgggcgt cgcgacgccc
     1921 tgctcgacca gccacagcgc ctcgcgtagc agggcggtct ggagccggtt ccagaggaag
     1981 ccgggcacct ccttgcgcac ctcgacgggg cgcttgcccg cggactcgag cgcgcgcctg
     2041 gtgcgctcga ggaccgccgg gtcggtccgc tcgccggcga cgagctcgac gagcggcatg
     2101 agcagcggcg ggttccagta gtgggtgccg accgtccgct cgggcgcgcc gatgcggtcg
     2161 ccgagcgcgg tgacgctgag cgacgacgtg ttggtcgcca ggaccgcctc cggcatcgcc
     2221 gccgccgggc cgcgcagcac cgacgccttc agctcgaggt cctcggggac cgactcgacc
     2281 acgaggtcgg ccggctcgcc gccggccgcc gtgaggtcgc cgacggtgac ccggcccgcc
     2341 gccgcctccg cctccccggg cgagaagacg ccgaggtcgc gcgcctcggc gaggacccgg
     2401 ccgaccgccg agcgcgcacg cgcggggtcc cgcgccgcga aggcgaccgc gtggcccgct
     2461 ctcgcgtact cgcagccgat ctgcgatccc atcaggccgg cgccgatcac caggacgcgc
     2521 agcctcgccg cgggcggcgg tgggcggcgg cgccccgggg ggccagcgcc cccggcgccg
     2581 tgctcccgct cgtgctgctc catggtcgcc agcctacctg ctccgggcgg cggtccgcgg
     2641 ccggggcggg agcccgcatg ccgcgctggc attcccggca cacgcccagc atggcagact
     2701 gcgccgaggg gggcgccggc ccgcggtcaa ggaggctcgc atggagcgca ccaggtcgtc
     2761 gccggggccc gcccgcggtg ccatcggcct gcgcgaggtg ctgttccagt ccatcaccca
     2821 cgccgagcgg gcctgaccac ggcgatgcgc ttccaggagc cgttcttcca ggagccgccc
     2881 gggttgcgga acacctaccg ctcggacgtg gtccttcgcg agtacctcga gcggctgctc
     2941 cccgcggaga tccgcgcgca ggtcgagccc gagctcgacc agatgggcga ggcggcggcc
     3001 ggcgagctgg ccctcctcgc ggaggaggcc gaggggtcgc cgcccaggct ggtgcagtac
     3061 gacccgtggg gcaggcgcat cgaccggatc gagctgagtc ccgcctggac ggagctgcac
     3121 gccgtccagg cgcgagctgg cctctgcgcc ctcccgtacg aggaccgcca cggccgctac
     3181 gaccgcatcg tccagcatgc gctgatccac ctctacgctc cctcctccgc cgtctgcacc
     3241 tgcccggtgg cgatgaccga cgcggccgtc cgcgtgctgg tcgaccacgc gcccgccgag
     3301 ctccgcgagc gggtcgtccc tcgcctgacg agccgcgacc ccgagcgcgc gtggacgtcg
     3361 ggtcagtgga tgaccgaacg cgagggcggc tccgacgtcg gccgcacctc gacggtggcg
     3421 cggtgccacg aagcgggcga ctggcgcctg tacggcgtca agtggttcac ctcggcgacg
     3481 accgccgact gcgcgctcgc gctcgcccgg cccgacggtg cgccggaggg cagccggggc
     3541 ctcggcctgt tcctcgtcga gcgggtcgac ccgcgcagcg ggcggacgca gctcggctcg
     3601 accatcctgg tgaaccgcct gaaggacaag ctcgggacgc gggcgctgcc gaccgcggag
     3661 ctcacgctgg cgggcgccca cgcgaccccg gtgggcagcg tcgacagggg tctcgcgaag
     3721 atcgcgggga tgctcaacgt cacgcggctg cacaacgggc tcagctccgc cgccggcatg
     3781 cggcgggggg tggagctggc ggtggcgtac gcccgggtcc gccaggcgtt cggccggccg
     3841 ctcatcgacc tgccgctgca cagggcgacc ctcgccgacc tggcggtcga ggcggaggcc
     3901 gggttcgccc tcgtgtcgcg cgggatggag ctgatgggtc gggtggagca cgggctggcg
     3961 agcgagcccg agcagcgcgc gctgcgggcg ctcgtcccga ccgtcaagct gctcaccgcc
     4021 aaggacgccg tcgcccacgc cagcgaggtg ctcgaggcct tcggcggtgc cgggtacgtc
     4081 gagagcaccg gcgtcccgcg gctgctccgc gacgcgcagg tgctgcccat ctgggaaggc
     4141 acgacgaacc tgctcagcct cgacctgctg cgcgcggagt ccagggagga ggccgtcacc
     4201 gcgctcctgg gggacctgtc cgagcagatc gccgccgtgc gcgcggcgcc ggagacgggc
     4261 gtcagcgtgg acctcgtgga gcgttcgcac gcggccctgc tggagcgcgt ccgtgcctgg
     4321 ccggacgctg acggcgacgc gctccaggag cagatgcggg acttcgcgct ccgcctcggc
     4381 cgcacctgcg ccgcggcgct gctcgcccac catgccgccc accgccgcgc gcaccggggc
     4441 gacgaccggg ccgcccaggt cgcacactgc tatgccgagc gccggctgcg ggcggccgtg
     4501 gcgtgaggcg taccgacagg aggagccagt ggagaccttc aaggcgctcg tcgccgagcg
     4561 gcacgacgac cgggtcgacc ggcggaccga ctccctgacc ctcgaagacc tgccccaggg
     4621 cgaggtgacc gtgcgggtgg cctggtcgag cgtcaactac aaggacgccc tcgccgtgag
     4681 cccgaagggc cgcgtggcgc ggatctcgcc gctcgtgcca gggatcgacc tggccgggga
     4741 ggtgaccgcg agcgacgcgc ccgagttccg ccccggggac cgggtgctcg cccacggcta
     4801 cgacctcggg gtgagccact tcggagggtt cgcggagtac gcccgcgtcc cggccggctg
     4861 ggtggtcccg ctcccggccg gcctgagcct gcgccaggcg atggccctcg gcacggccgg
     4921 ctacaccgcc ggcctgtcgg tgcagtccct cgaggagcgc ggcctccacc ccggcgccgg
     4981 gccggtgctg gtgctcgggg cgagcggcgg ggtcggctcc accgcggtcg gcatcctcgc
     5041 gggccgcggc tacgaggtat gggcgagcac gggcaagcgg gaggaggagg cgttcctgcg
     5101 gggcctcggc gcgagcgagg tgctgccgcg cgaggagacc tccgccgagg gctcgaggcc
     5161 gctcgagccg gcccgctggg cgggctgcgt ggacccggtc gggggagcct ccctcgccta
     5221 cgccctgcgc accctgcggg agggggcggc ggtcgcggcg agcggcaaca ccggcggggt
     5281 ggagctgcat accaccgtcc tgccgttcat cctgcgcggc gtcgccctgc tcggcgtcga
     5341 ctcggcgcac accccgatcg agcgccgccg ccgcgtctgg gagcggctgg cgggcgacct
     5401 gcgcccgagg ggcctcgacg agtggatcac ccgggaggtc tcgctcgacg acctcggcgg
     5461 cgtgctcgac gacaccctgg cgggccgggg caggggccgg accgtggtcc gggtgggcgg
     5521 cgggtagccc gtgcgggtgg gcggaacgct tcgcgatata taggcccgaa ccagcgggtt
     5581 cgtcctttcg gcggctgatg ttgtagaaga agcgctgcat gccgcgcgtc gccccccgtc
     5641 atgcgcgtcg ccgtgcgcac cggcccttca gaggactcgc cgtatgggag aatcgccttg
     5701 agcgttggac cactgccgcc gacctccgcc gccgccgacc agtggctgct gcggttccga
     5761 tcgatcctgg tcgccattct cgggacgatg gtcgcgggca tcgcgtgcct cgccttctac
     5821 accagcttcg aggcgatccg ggcctacgcg atgcggtcgg ccggcatcgc cccggagcac
     5881 gggtgggcga tcccgctcct cgtggactcg ttca
//
LOCUS       DASPYY010000790         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_697503, whole genome shotgun sequence.
ACCESSION   DASPYY010000790 DASPYY010000000
VERSION     DASPYY010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697503"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..>1501)
                     /locus_tag="VEP73_12350"
     CDS             complement(416..>1501)
                     /locus_tag="VEP73_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYT27263.1"
                     /translation="RSRKTAFPGRQIAEFFGVTSGPLAEHIDANILNLGGERHRRLRA
                     LVGPALTPRAADRWRPVMRGFLEQLWAQAAAYGGCDFVAAVARPYPSLTIATVLGAPL
                     QDAGRLHTWSTWVQRQFDIRALETSREDIERAVTEVREYVTGLLEARRAAPGDDLISD
                     LLAARDETGGGRLSAEESVQLVTNLLAGGIDTTAGQLAHAIRIFAEHPGQWARLAADP
                     GLVPRAVDEVLRFEPVTPFTARICLEQVEYRDVVFPAGTIVAVCAERANRQGEDGENF
                     DITAPRESRLLTFGAGIHYCLGANLARAELEEALAFLAPRTPGLALDGPPQLGGVEGI
                     YGVDKLPITWSDSSTMNPNHGPAPDVS"
BASE COUNT          198 a          542 c          567 g          194 t
ORIGIN      
        1 cccaccatga acaggctcat gcagcagacg gcgagcatca ggccgcggcg accgcggccc
       61 gcggcgggca gggacacggg agcagcgggc tggggtcccg gagcagcggg ctggggtccc
      121 ggagcggcgg gctggggtcc cggaggggcg ggctggcgcg ggcgcggacg cgcgcccgca
      181 gcgaggcccg ggtagggatc cgaccctgga tgcggtcccc gggccgggta tgtacccgga
      241 gcggggtgac ccgcggcggg gtggcccgcg gcgagctcga gtcccaggac gggcacaggc
      301 atccgttctg gtagtcgagc gggcacgtta cgtgacctcc ggtgagtgga cttcgctggt
      361 gcctgcatcg tgcgaacccc tcaggtcgta ctcgccgcgg acgagtaccg cctacttatg
      421 acacgtccgg cgccggaccg tggttcgggt tcatcgtgga tgaatccgac caggtgatcg
      481 ggagcttgtc gaccccgtag atgccctcga ccccgcccag ctggggcggc ccgtccaggg
      541 cgagtcctgg ggtgcgcggc gcgaggaacg ccagcgcctc ctcgagctcg gcccgggcca
      601 ggttcgcgcc caggcagtag tggatgcccg cgccgaaggt gagcagccgg ctctcgcgcg
      661 gcgcggtgat atcgaagttc tcgccgtcct cgccctggcg gttggcccgc tccgcgcaga
      721 ccgccacgat cgtgcccgcc gggaagacga cgtcgcggta ctcgacctgc tccaggcaga
      781 tccgcgcggt gaacggcgtg accggctcga accgcagcac ctcgtcgacg gcgcgcggca
      841 ccaggccggg atcggccgcc agccgcgccc actggccggg atgctcggcg aagatccgga
      901 tcgcgtgcgc gagctggccc gcggtggtgt cgatgccccc ggccaggagg ttcgtcacga
      961 gctgcacgga ctcctcggcc gacagccgcc ccccgcccgt ctcgtcgcgg gcggcgagca
     1021 ggtcgctgat caggtcgtcg ccgggtgccg cccgcctggc ctcgagcagc ccggtgacgt
     1081 actcccggac ctcggtgacc gcccgctcga tgtcctcccg gctggtctcg agcgcccgga
     1141 tgtcgaactg ccgctgcacc cacgtcgacc aggtgtgcag ccgccccgcg tcctggagcg
     1201 gcgccccgag cacggtcgcg atcgtcagcg acgggtaggg ccgggccacc gcggccacga
     1261 agtcgcatcc gccgtaggcg gcggcctgcg cccacagctg ctccaggaag ccgcgcatca
     1321 cggggcgcca gcgatcggcc gcgcgcggcg tgagcgccgg ccccacgagc gcccgcagac
     1381 ggcgatgccg ctcgccgccg aggttcagga tgttagcgtc aatgtgctcg gccagcgggc
     1441 cgctggtgac gccgaagaac tcggcgatct ggcggccggg gaacgcggtc ttcctcgacc
     1501 t
//
LOCUS       DASPYY010000791         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1962159, whole genome shotgun sequence.
ACCESSION   DASPYY010000791 DASPYY010000000
VERSION     DASPYY010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962159"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VEP73_12355"
     CDS             <1..630
                     /locus_tag="VEP73_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HYT27264.1"
                     /translation="LGVLKGMQAVAEELWKRHQLDGLHVVVQGVGKVGYHLCRMLHEA
                     GARLTVADVDVDQVARAVNEFGADTAEPGKVHAIGCDIFAPCALGSAVNDDTLPELSC
                     AAIAGSANNVLARPEHGYALRELGILYAPDYVVNSGGLIQVADELQGYSADRARAKVE
                     GIFGMLRTIFHRAGQEGIPTSEAADRAAEERMREISRLRLVRVLPGLAR"
     gene            complement(651..1529)
                     /locus_tag="VEP73_12360"
     CDS             complement(651..1529)
                     /locus_tag="VEP73_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYT27265.1"
                     /translation="MTFSIAAFDPASASWGVAVASKFLAVGSAVPWGRAGAGAVATQA
                     AANLSYGPDGLDLLAEGMAAGEVIERLTTADRGRDHRQLGVVDARGGAATFTGGACLD
                     WAGGTAGEGFAAQGNVLTGPEVVDAVVAAYQRSAADRVGTAPARALARRLVGALAAGD
                     AAGGDRRGRQSAALRVWKAGAAYGGTLDIAVDLRVDDHHDPVGELARLLDLHELYFGE
                     PDPARLEPLEGELAGQVAACLERLGYPTRGGAGLPGALEAWAGTENFEERLVPGKLDP
                     VVWRQLRRQAGAARDA"
BASE COUNT          210 a          677 c          639 g          189 t
ORIGIN      
        1 ctcggcgtgc tcaagggcat gcaggcggtc gccgaggagc tgtggaagcg gcaccagctc
       61 gacgggctcc acgtcgtcgt ccagggcgtc ggcaaggtgg gctaccacct gtgccgcatg
      121 ctccacgagg cgggcgcccg cctcaccgtg gccgacgtcg acgtcgacca ggtcgcccgg
      181 gcggtcaacg agttcggcgc cgacaccgcc gagccgggca aggtccacgc gatcgggtgc
      241 gacatcttcg ccccgtgcgc gctcggctcg gcggtcaacg acgacacgct gccagagctc
      301 tcgtgcgccg ccatcgccgg gtcggcgaac aacgtgctcg cccgccccga gcacggctac
      361 gccctgcgcg agctcgggat cctgtacgcg cccgactacg tggtgaactc cgggggcctc
      421 atccaggtgg ccgacgagct gcagggctac tccgccgacc gggccagggc caaggtcgag
      481 gggatcttcg ggatgctgcg caccatcttc caccgggccg ggcaggaggg catcccgacc
      541 agcgaggcgg ccgaccgcgc cgccgaggag cggatgcggg agatctcccg cctccgcctc
      601 gtccgggtgc tgcccggcct ggccaggtag ccgcccggcc gcgcacaggg tcaggcgtcc
      661 cgcgcggccc cggcctgccg ccggagctgc cgccacacca ccgggtcgag cttgccgggc
      721 acgagccgct cctcgaagtt ctcggtgccg gcccaggcct ccagcgcccc ggggagcccc
      781 gcaccgcccc gcgtcgggta accgagccgc tcgaggcagg cggccacctg cccggcaagc
      841 tccccctcga ggggctcgag cctggccggg tccggctcgc cgaagtacag ctcgtggagg
      901 tcgaggaggc gggccagctc gccgaccggg tcgtggtggt cgtcgacgcg gaggtccacc
      961 gcgatgtcga gcgtcccgcc gtacgcggca ccggccttcc agacccggag ggcggcgctc
     1021 tggcggccac ggcggtcgcc cccggccgcg tcgcccgccg cgagcgcccc gacgagccgc
     1081 cgtgccagcg cccgggctgg cgcggtcccc acccggtccg cggccgaccg ctggtaggcc
     1141 gcgaccaccg cgtccaccac ctcggggccg gtgaggacgt tgccctgggc ggcgaacccc
     1201 tctccggccg tcccgccagc ccagtccagg caggcgccgc cggtgaacgt ggccgcgcct
     1261 ccccgcgcgt ccaccacccc gagctggcgg tggtcgcggc cgcggtccgc cgtggtcagc
     1321 cgctcgatca cctccccggc ggccatgccc tcggcgagca ggtcgagccc gtccggcccg
     1381 tacgagaggt tggccgcggc ctgggtggcg accgcgcccg ccccggcccg gccccagggg
     1441 acggccgagc ccacggccag gaacttggag gcgacggcga ccccccagga ggcggacgcg
     1501 ggatcgaagg cggcgatgga gaaggtcaag aggcctccgg cggtggctgg gcggccgagc
     1561 gccggcagtc taccgccgtc ccaccggcgc gggccgggtg gcgtcgtgcc cggcgggggg
     1621 ccggtagagt tgtgccgctc ccgtctcgaa gggccgcccg atggacccaa ccgaagcccg
     1681 gacgccgcgg cccccgcggt ggctcgccgt cagca
//
LOCUS       DASPYY010000792         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_413131, whole genome shotgun sequence.
ACCESSION   DASPYY010000792 DASPYY010000000
VERSION     DASPYY010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413131"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2149
                     /locus_tag="VEP73_12365"
     CDS             <1..>2149
                     /locus_tag="VEP73_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27266.1"
                     /translation="GPARFRSQIQALADDPGLVPVGDLLDTFAPRGGVIPPNRAVPVQ
                     GTPITLPARTNLPGVVLPVTLRVGARTTAGQMTTSLSTFVVDVNEQVRNRLHAALLVP
                     LHDRSHRTSNGDFVDTNLAAQLSARSPLGAMAAELARPESANVTMMIDALLTEEAKAM
                     GGAWAVRPPNRPGIEVPAGSLRSRTSQEFLANLGRAISRNRPGTLPYADADLPALVQA
                     GLDSEVLPQVLAGQRIIRQQTGVSPDASLAWPVDGAIDATTLRPLAIASADTVVLDAD
                     RLSRPAEADVSQNATVDLSAGAGQPRRALVPDEVLATALSDRRATTAPVEWAQRILAE
                     TAVVWLEKPGGDNPRARGILLAPRQHTWRPAPLFFQTLVRGLASAPWLELQHASDLAD
                     QVRQGPDTTPRELLPVTAADVALGLPSDFLHGVANARAHLNSFVRVVGPDYPQAAVYD
                     RNLLIAESSDWRGSAHATRRTTFARSVDNGIAALYRKVSVEKTRFTLTAREGPIPIRV
                     TNATDQRLTLLIRVSSPKVDVPAATQSFTLEPMRGTTQTVLVRTRTTGTFPIRVDVLT
                     PDGAYTVASTDMVLVSTAFSKVALALTGGAAGFLLLWWSRRLGKSRREGRLVRGDGAG
                     GDAMAVAGPPGPVDPLDSGPVAAAPGGGPPTDGPPPEEPSLARSTAVMAAGTLLSRVT
                     GMLRVIVLVATLGVAESRLADTYNVANTTPNIIY"
BASE COUNT          294 a          852 c          749 g          254 t
ORIGIN      
        1 ggccccgccc gcttccggtc ccagatccag gcgctcgccg acgacccggg cctggttccc
       61 gtcggcgacc tcctggacac gttcgccccc cggggcggcg tgatcccccc caaccgtgcc
      121 gtcccggtgc agggcacccc gatcacgctc cccgcccgca cgaacctgcc cggcgtggtc
      181 ctcccggtca ccctgcgggt gggcgcgcgg accaccgccg gccagatgac gacgtcgctc
      241 agcaccttcg tcgtcgacgt gaacgagcag gtccgcaacc ggctgcacgc cgcgctgctg
      301 gtcccgctgc acgaccgctc gcaccggacc tcgaacgggg acttcgtcga caccaacctc
      361 gccgcacagc tctccgcccg gtcgccgctc ggggcgatgg cggccgagct ggccaggccg
      421 gaatcggcca acgtcacgat gatgatcgac gccctcctca ccgaggaggc gaaggcgatg
      481 ggcggcgcct gggccgtccg ccccccgaac cgcccgggga tcgaggtccc tgccggcagc
      541 ctccggtccc ggacgtccca ggagttcctg gccaacctgg gcagagcgat cagccggaac
      601 cggccgggca cgctgcccta cgccgatgcc gacctgcccg ccctcgtcca ggccgggctc
      661 gactccgagg tactgcccca ggtcctggcc gggcagcgca tcatcaggca gcagaccggc
      721 gtcagcccgg acgcgtcgct ggcctggccg gtcgacggcg cgatcgacgc caccacgctg
      781 cggccgctgg ccatcgcctc cgccgacacg gtcgtgctcg acgcggaccg gctgtcgcgg
      841 ccggccgagg ccgacgtgag ccagaacgcg accgtcgacc tgagcgccgg ggccggccag
      901 ccgcgacgcg ccctggtccc cgacgaggta ctcgcgacgg cgctgtcgga ccggcgggcg
      961 accacggccc cggtcgagtg ggcccagcgc atcctcgccg agaccgccgt cgtctggctc
     1021 gagaagccgg gcggggacaa cccgcgggcg aggggcatcc tgctcgcgcc gcggcagcac
     1081 acctggcgcc cggccccgct gttcttccag acgctggtgc gagggctggc cagcgccccc
     1141 tggctcgagc tgcagcacgc ctccgacctc gcggaccagg tgcgacaggg gccggacacg
     1201 acgcccaggg agctcctgcc cgtcacggcc gcggacgtgg ccctcgggct gccgagcgac
     1261 ttcctgcacg gtgtcgccaa cgcccgcgca cacctgaaca gcttcgtccg cgtggtcggc
     1321 ccggactacc cgcaggccgc cgtctacgac cgcaacctgc tgatcgccga gtcgagcgac
     1381 tggcggggca gcgcgcacgc caccaggcgg accaccttcg cccggtcggt cgacaacggc
     1441 atcgcggcgc tgtaccggaa ggtgagcgtg gagaagaccc ggttcacgct gaccgcccgc
     1501 gaggggccga tcccgatccg ggttacgaac gcgaccgacc agcggctgac cctgctgatc
     1561 cgcgtgtcct cgccgaaggt ggacgtgccg gccgccaccc agtcgttcac cctggagccg
     1621 atgcgcggca ccacccagac cgtgctggtg cggaccagga cgaccggcac cttcccgatc
     1681 cgggtcgacg tgctgacccc ggacggcgcg tacacggtgg cgagcaccga catggtcctg
     1741 gtgtcgacgg cgttcagcaa ggtcgccctc gccctgaccg gaggggccgc cgggttcctg
     1801 ctcctgtggt ggagccggcg cctcgggaag agccgccgag aggggcgcct ggtcaggggc
     1861 gacggcgcgg gcggggatgc catggccgtg gccggcccgc ctggcccggt ggaccccctc
     1921 gacagcgggc ccgtggcagc cgcgcccggt ggcgggccgc cgacggacgg cccgccaccc
     1981 gaggagccga gcctcgcgcg ctcgaccgcc gtgatggcag ccggcacgct gctcagccgg
     2041 gtgacgggca tgctccgcgt gatcgtgctc gtcgcgaccc tcggcgtggc cgagagcagg
     2101 ctggccgaca cctacaacgt cgccaacacc accccgaaca tcatctacg
//
LOCUS       DASPYY010000793         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1117987, whole genome shotgun sequence.
ACCESSION   DASPYY010000793 DASPYY010000000
VERSION     DASPYY010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117987"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEP73_12370"
     CDS             <1..569
                     /locus_tag="VEP73_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT27267.1"
                     /translation="TAAPRGAPGRVTVTPLPAATVRVRPIRPSDGPVLQAGMGELSEQ
                     TRWLRFHMPISRLSDAQLRLLVDVDHHDREALLAEVRGEGGEWEPVGVARYARVAADR
                     ADLAVVVADAWQGRGIGGLLIERLTAVARGEGIVAFVAQVLSENRRVLALLRRPGHRV
                     ELTMAGPITDAVWWLTDPPATAALQGAR"
     gene            650..1114
                     /locus_tag="VEP73_12375"
     CDS             650..1114
                     /locus_tag="VEP73_12375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYT27268.1"
                     /translation="MPVAAAARTMAGIDVLRAIVRGDLPAPPIAGLMDLRIAEVAEGR
                     VVFETQPAEYHYNPIGMVHGGLACTLCDSAMGCAVQSTLPAGTGYTTLELKVNFVRPM
                     TVTTGVVRCEAAVVSAGSRVATAEARVVDAAGRLYSHATATCLIFGPPADAG"
     gene            complement(1181..1357)
                     /locus_tag="VEP73_12380"
     CDS             complement(1181..1357)
                     /locus_tag="VEP73_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27269.1"
                     /translation="MFSLTKIGADGIARELEARWVDETVLGLTVFVATEHCMIPLDRA
                     EARRFARWLAGQVE"
BASE COUNT          183 a          578 c          554 g          200 t
ORIGIN      
        1 ccaccgccgc gccgcgcggc gcgcccgggc gggtcaccgt cacgccgctg ccggcggcga
       61 ccgtccgcgt ccgcccgatc cgtccctcgg acgggcccgt cctccaggcc gggatgggcg
      121 agctgtccga gcagacccgg tggctgcgct tccacatgcc gatcagccgc ctgagcgacg
      181 cgcagctccg cctcctcgtc gacgtggacc atcacgaccg cgaggcactc ctggccgagg
      241 tgcgcggcga gggcggggaa tgggagccgg ttggcgtcgc ccgctacgcc agggtggccg
      301 ccgaccgggc cgacctcgcc gtcgtggttg cggacgcctg gcagggccgc ggcatcggcg
      361 gcctcctgat cgagcggctc accgccgtcg cccgcgggga ggggatcgtc gcgttcgtcg
      421 cgcaggtgct ctccgagaac cggcgggtcc tcgcgctcct gcgccgcccg ggtcaccggg
      481 tggagctcac catggccggc ccgatcaccg acgccgtctg gtggctcacc gacccgccgg
      541 ccaccgcggc cctgcagggt gcccggtgac ccggccagag gacgggaccg ctgtcctgcc
      601 gagctcgaac ggcccggcgc ggtcccggac gcacacctgg caagacccga tgccggtcgc
      661 ggccgcggcg aggacgatgg cgggcatcga cgtcttgcgg gcgatcgttc gtggcgacct
      721 gcccgccccg ccgatcgccg gcctcatgga cctgcggatc gccgaggtgg ccgagggtcg
      781 ggtggtgttc gagacccagc cggccgagta ccactacaac ccgatcggca tggtgcacgg
      841 ggggctggcg tgcacgctct gcgactcggc gatgggctgc gcggtgcagt cgaccctgcc
      901 cgccgggacc ggctacacca cgctcgagct gaaggtgaac ttcgtgcgcc cgatgacggt
      961 gacgaccggc gtcgtgcgtt gcgaggctgc ggtggtctcg gccggctcgc gggtcgccac
     1021 ggccgaggcc cgggtcgtcg acgcggccgg caggctctac agtcatgcga cggcgacctg
     1081 cctgatcttc gggccgcccg ccgacgccgg ctgaccgagc ctggcgcgcg gcgggtcacc
     1141 gcgggcgcca ggcgtccacg ggcgggggag cggacacctc ctactcgacc tggccggcga
     1201 gccagcgcgc gaaccgtcgt gcctccgcgc ggtcgagcgg gatcatgcaa tgctccgtcg
     1261 cgacgaagac ggtgagcccg agcaccgtct cgtccaccca cctcgcctct agttcacgag
     1321 cgatcccatc ggcgccgatc ttggtcaagc tgaacaagtc ggggtctcgc tctgaacccc
     1381 gcggacctcc gactggaccc gctggccgcc tgcgccgagg cccgcaccgc tgtggtggcc
     1441 ggagccccca cccgcgaccc cgagtccggg gggctgcaca tctctgccct ggtcctgggc
     1501 cgcgacggcc gattc
//
LOCUS       DASPYY010000794         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2804208, whole genome shotgun sequence.
ACCESSION   DASPYY010000794 DASPYY010000000
VERSION     DASPYY010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804208"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VEP73_12385"
     CDS             <1..467
                     /locus_tag="VEP73_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT27270.1"
                     /translation="AYGELLLRYFVAWIGDRPAAEELTGRVFAAAVERLPRFDGSTAT
                     LGSWLFGIARHELGGDAPGEPLGGHAPGPAPGAAERHGPGEAARMRYSHTVAAARQLA
                     ADQREVVLLRMIGLTAAETAQVVGTTAASVKTLQRRGLAGLARLLGLPGPGG"
     gene            520..1461
                     /locus_tag="VEP73_12390"
     CDS             520..1461
                     /locus_tag="VEP73_12390"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HYT27271.1"
                     /translation="MANDPARVDDELDVVLDGRRHLYRSELGPEVQAARLLAEELRAI
                     ELPSGVAERHVAMALERARSSSARAGGRSLWRALTAAVAAAAIFGVPASLASGRSLPG
                     QPLYPLKRTIEAADLAFTRDPCKEADLQLRIAHTRMDELGALLHERDGAHVLGAVQAV
                     QSAFLAANGAVARALGAEGSNRETAALVAELGTVKSAMKRQLAGIVAAGDPATPKQAV
                     VTASSILATSTPTPPPPPPARQTTTTATPSTTDVPPTTAPPPTTAPATTAPPATTAPP
                     TTAPPVTTTATAPAGPPSRGSGTDAHGGGPEVATSAP"
     gene            1561..1785
                     /locus_tag="VEP73_12395"
     CDS             1561..1785
                     /locus_tag="VEP73_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HYT27272.1"
                     /translation="MSTTETRPAPSDSDTQEGDGYAHYANKTDITRAAIEGGLVIALC
                     GFRFPPIRDPQRFPVCPRCKELAALLWGAD"
BASE COUNT          238 a          696 c          673 g          194 t
ORIGIN      
        1 gggcctacgg cgagctgctg ctgcggtact tcgtcgcatg gatcggcgac cggccggccg
       61 ccgaggagct caccgggcgg gtgttcgccg ccgccgtgga gcgcctgccg cgcttcgacg
      121 ggtcgaccgc gacgctcggg agctggctgt tcggcatcgc ccggcacgag ctgggcggcg
      181 acgctccagg tgagccgctc ggcggccacg ctcccgggcc cgcgccgggc gccgccgagc
      241 ggcacggtcc gggcgaagcg gcacgaatgc ggtacagtca tacggtagcc gccgcccggc
      301 agctcgcggc cgaccagcgc gaggtggtgc tgctgcgcat gatcggcctc acggccgccg
      361 agacggccca ggtcgtcggg acgacggcgg cctcggtcaa gacgctgcag cggcggggcc
      421 tggccggcct ggcgcggctg cttggactgc ccgggcccgg ggggtaggct ggccgggccg
      481 tccgggagca cgggacggga ggaggagggg ggagccctga tggcgaacga ccccgccagg
      541 gtcgacgacg agctggacgt cgtgctcgac gggcgccgcc acctctaccg gagcgagctc
      601 ggaccggagg tccaggcggc caggctgctc gccgaggagc tgcgggcgat cgagctgccg
      661 tccggggtgg ccgagcggca cgtcgccatg gcgctcgagc gggcgcgcag ctcctcggcg
      721 cgggcgggcg gccggtcgct gtggcgcgcg ctgaccgccg cggtcgccgc ggccgccatc
      781 ttcggcgtcc ccgcctcgct cgcgtccggg cgctccctgc ccggtcagcc cctctacccg
      841 ctcaagcgga cgatcgaggc ggcggacctc gcgttcacgc gcgacccctg caaggaggcc
      901 gacctgcagc tgcggatcgc ccacacccgc atggacgagc tgggcgcgct gctccacgaa
      961 cgcgacggcg cccacgtcct cggcgccgtc caggcggtgc agtcggcgtt cctggcggcc
     1021 aacggcgcgg tcgccagggc gttgggggcg gaagggagca accgcgagac cgccgccctc
     1081 gtggctgagc tggggaccgt caagagcgcg atgaagaggc agctcgccgg tatcgtagcg
     1141 gcaggcgacc cggcgacgcc gaagcaggcc gtcgtcaccg cgtcgtcgat cctggcgacc
     1201 agcacgccca cgccgccgcc cccaccaccc gcccggcaga cgacgaccac ggccacaccg
     1261 tccaccacgg acgtgccgcc caccacggcc ccgccgccga ccacggcgcc ggcgacgacc
     1321 gcgccgcccg ccaccacggc cccgccgacg accgcgccgc ccgtcaccac cacggccacg
     1381 gccccggccg ggccgccgag ccggggcagc ggcacggacg cccacggcgg cggtcccgag
     1441 gtcgccacga gcgcgccgta gccgcgaggg ccccggcagg tcgccggggc ctcgtcacgg
     1501 tcgcggcgca ctggtcacgg gccgaccctc cggacgaaga tgctggaagg agccttgcac
     1561 atgagcacga ccgagacgcg ccctgccccg tccgactcgg acacgcagga gggcgacggc
     1621 tacgcccact acgccaacaa gaccgacatc acccgcgccg ccatcgaagg cgggctcgtg
     1681 atcgcattgt gtggtttccg atttcctcct attcgcgatc cccagcgctt ccccgtctgc
     1741 ccgaggtgca aggaactggc cgctctgctg tggggtgccg actgagcgcc tgaggcggcc
     1801 c
//
LOCUS       DASPYY010000795         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1680540, whole genome shotgun sequence.
ACCESSION   DASPYY010000795 DASPYY010000000
VERSION     DASPYY010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680540"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VEP73_12400"
     CDS             <1..505
                     /locus_tag="VEP73_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27273.1"
                     /translation="PERVVRLGIAHVPEGRRLFPGLTVRENLLAGAYHRHQRSAALQH
                     DLDWVIGLFPVLGERLGQVAGRLSGGEQQMCAIGRGLMSRPRLLLIDELSLGLAPRLL
                     EVFLDRLREIAAAGTAVLAVEQDVDAALDLAAHAYVLETGRIVAEDDSDRLRADPRVR
                     EAYLGLA"
     gene            606..>2725
                     /locus_tag="VEP73_12405"
     CDS             606..>2725
                     /locus_tag="VEP73_12405"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYT27274.1"
                     /translation="MQFVYETGGQAWELDLRVEHPDACVADLAAALRPPVEHAGERSD
                     AAHGRPAEPGGELWIDGRRTPGDLGLGEAGLVAGAVVRPGADPDAAFGIGVGAGTGTA
                     GAGASSPRGAYGVTPPLPAVAVVRVVGGLDAGASIPVGPGSVVVGRLGDGGIRLADPG
                     VSREHCRLDVGAGGAVTISDLRSTNGTEVDGEPVLPGAPVPVAPDQVVTLGDSVQLRV
                     LPFMLTGPVVALDPLREAGAAGTLPFNRAPRTALPADPQPLGAPQQPNKRAGGTFSVA
                     SILGPLVMAGAMVALLHDLRFAAFALLSPVMVLGNWFETRTKGRRSLRRDLRDFSRDL
                     DELRASLGERRQAEIRRRRALLPDPAEISFRATAPSPWLWERRASSGDFLVLSAGLAD
                     LPWQPPVRDDRGTPPPEVAELLAELGELPWVPVPVSLAPGGVVGLEGDRYAALAVARA
                     LLCQAVTTSGPADVTVAVFCDQDRAADWDWAKWLPHVLDQRSGGSSRLLAVGDEAVDA
                     LAKELLDAGRAQGQAQDGPGGNKQHSGPVLLLVVDGATLTEGRPCPLRELLAGRGGPA
                     GGIVLTNRLPALCTVTMMVAPEGHAELRRPATAELVPDLLAAGVDEPTARTLARALAR
                     FEDPELGIEGAGLPDQVPLLPLLELPELTPQALAARWRVGTSTLRAGAALGVTEYGVF
                     RIDLDDDGTHGLIAGTTGSGKSELL"
BASE COUNT          309 a         1014 c         1055 g          347 t
ORIGIN      
        1 accagagcgc gtcgtgcggc tcgggatcgc ccacgtgccc gagggccgcc gcctcttccc
       61 cggcctcacc gtgcgcgaga acctgctggc cggcgcctac caccgccacc agcgcagcgc
      121 cgccctccag cacgacctgg actgggtcat cggcctgttc cccgtgctcg gcgagcgcct
      181 cgggcaggtc gccggcaggc tctccggcgg cgagcagcag atgtgcgcga tcgggcgcgg
      241 gctgatgagc cgcccccggc tgctgctgat cgacgagctg tcgctcggcc tcgccccgag
      301 gctgctggag gtgttcctcg accgcctgcg cgagatcgcc gccgccggga ccgccgtgct
      361 cgccgtcgag caggacgtgg acgcggccct cgacctggcc gcccacgcct acgtgctgga
      421 gaccggccgc atcgtcgccg aggacgacag cgaccgcctc cgcgccgacc ctcgcgtccg
      481 ggaggcctac ctcggcctgg cataaccggg gtcaggtagg cttccgcgca ggatcgaccc
      541 cccagtttga gccattagaa cccttagaac cctcagaacg ctcagagaag acgcggaggc
      601 tgtgcgtgca gttcgtctac gagaccggcg ggcaggcgtg ggagctggac ctgcgggtcg
      661 agcaccccga cgcctgcgtc gccgacctgg ccgctgcgct gcggccgccg gtcgagcatg
      721 caggcgagcg aagcgatgcc gcccatggga ggccggcgga gcccggcggc gagctgtgga
      781 tcgacggcag gcggacgccc ggtgacctcg gcctgggcga ggccgggctg gtggccgggg
      841 cggtggtacg gccgggcgcg gaccccgacg ccgccttcgg catcggggtc ggggccggga
      901 ccgggaccgc cggcgcgggc gcctccagcc ccaggggtgc ctacggggtg acgccgccgc
      961 tgccggcggt cgcggtggtg cgggtggtcg gcggccttga cgctggcgcc tcgatcccgg
     1021 tggggccggg atcggtggtg gtgggccgcc tcggcgacgg cgggatccgg ctggccgacc
     1081 cgggcgtgtc gcgggagcac tgccgcctcg acgtcggcgc gggcggcgcg gtgaccatca
     1141 gcgacctgcg ctccaccaac ggcaccgagg tcgacggcga gccggtcctg ccgggcgccc
     1201 cggtcccggt cgcgcccgac caggtggtca cgctgggcga ttcggtgcag ctccgcgtcc
     1261 tgccgttcat gctgaccggg ccggtggtgg ccctggaccc gctgcgggag gcgggcgcgg
     1321 ccgggacgct gccgttcaac cgggcgcccc gcaccgcgct gccagcggac ccgcagccgc
     1381 tgggagcgcc gcagcagccg aacaagcggg caggcgggac cttcagcgtg gcgagcatcc
     1441 tcggcccgct ggtcatggcg ggtgcgatgg tcgccctgct gcacgacctc cgcttcgcgg
     1501 cgttcgccct gctgagcccg gtgatggtgc tcggcaactg gttcgagaca cgcaccaagg
     1561 gccgccgcag cctgcggcgc gacctgcgcg acttcagccg cgatctggac gagctgcgcg
     1621 cctcgctcgg cgagcgccgc caggccgaga tccgccgccg ccgtgcgctg ctgccagacc
     1681 ccgccgagat cagcttccgg gccaccgccc caagcccgtg gctttgggaa cggcgtgcct
     1741 cctccggcga tttcctggtg ctgtcggccg gcttggccga cctgccctgg cagccgccgg
     1801 tccgcgacga ccgcggcacg ccgccgcccg aggtcgccga gctgctggcc gagctggggg
     1861 agctcccctg ggtgccggtc ccggtaagcc tcgccccggg cggggtcgtg gggctggagg
     1921 gcgaccgcta cgccgccctg gcggtggcgc gggcgctgct gtgccaggcg gtgaccacct
     1981 cgggcccggc ggacgtgacc gtggcggtgt tctgcgacca ggaccgggcg gcggactggg
     2041 actgggccaa atggctcccg cacgtgctcg accagcgcag cggcggctcc tcgcggctgc
     2101 tggcggtggg tgacgaggcg gtggacgccc tcgccaagga gctgctggac gccgggcgcg
     2161 cccagggcca ggcccaggac ggtcctgggg ggaacaagca gcacagcggc ccggtgctgc
     2221 tgctggtggt ggacggcgcc accctcaccg agggccgccc ctgcccgctg cgcgagctgc
     2281 tcgccggccg gggtgggccg gctgggggga tcgtgctcac caaccggctg ccggccctct
     2341 gtaccgtgac catgatggtc gcgccggagg gccacgcgga gctgcgccga ccggccaccg
     2401 ccgagctggt gcccgacctg ctcgctgctg gggtggacga gcccaccgcc cgcaccctgg
     2461 cccgcgcgct cgctcgcttc gaagaccccg agctgggcat cgagggggcc ggcctgcccg
     2521 accaggtgcc gctgctgccc ctgctggagc tgccggagct gacgccgcag gcgctggcgg
     2581 cccgctggcg cgtcgggacc tcgaccctgc gggccggcgc cgcgctcggg gtcaccgagt
     2641 atggcgtgtt ccgcatcgac ctggacgacg acgggaccca cgggctgatc gccgggacca
     2701 ctgggtcggg caagagcgag ctgct
//
LOCUS       DASPYY010000796         5625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2101480, whole genome shotgun sequence.
ACCESSION   DASPYY010000796 DASPYY010000000
VERSION     DASPYY010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101480"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..559)
                     /locus_tag="VEP73_12410"
     CDS             complement(11..559)
                     /locus_tag="VEP73_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27275.1"
                     /translation="MPSSRREPAEPAAVERFRPGGVLHAVASGLLELALALVLLGLSQ
                     LAGEANGDVAASLVAAAVLWAVLGVYSLATGALRARVWEAELDDEAVVLHGAAGMRRW
                     RYAELSAVEIGGGRTRLVTRDGRTRRVRGVRGGEQGRRFRARVLARATTAARGPAGAD
                     RLPGAGPDGGGGHGPGEPAPGT"
     gene            complement(592..1308)
                     /locus_tag="VEP73_12415"
     CDS             complement(592..1308)
                     /locus_tag="VEP73_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT27276.1"
                     /translation="MRPSWRLPVYALAKALVPPVIRFWVRLDMRGSLDIPPRGPVIIA
                     ANHVSYFDPLCLGTFIDSAGRQVRFLAKSELFEGRLLRWILRGAGQIPVYRESRDAAK
                     SLAAAVAAMQEGAAVAIYPEGTTTRNADFTPMPAKNGVARLAALTGAPVVPVGMWGAH
                     LLFTRGKLGPFRRGIRVVIRAGPPMDLGLAPDAPLEAVNAARDRVMAAIGALVDEARA
                     GWRPPAWYRRREAGQSLPPP"
     gene            1427..2080
                     /gene="cofC"
                     /locus_tag="VEP73_12420"
     CDS             1427..2080
                     /gene="cofC"
                     /locus_tag="VEP73_12420"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HYT27277.1"
                     /translation="MPTPLLPVKALADAKGRLGPSLTPIERRLLTIAMLEDVVAALQA
                     VPGLGRPVVVSPDREVWRRADAIGCRVVEEVGTPAGLNASLATAAVAARAGDGAGDGL
                     VVVAADLPLATPGAISRVLEAAAAAPVVVVPDRDGGGTNVLAWRGPAPFRPAFGPDSA
                     RRHLALPGAVRLDEPALALDLDTVDDLRAVAAGVDPASVTGRRLRDLRLPARLERAG"
     gene            2118..2336
                     /locus_tag="VEP73_12425"
     CDS             2118..2336
                     /locus_tag="VEP73_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27278.1"
                     /translation="MLQGTVKSFDPKTRSAVVLTDARVEYAVPPEAFGRSGLRLLSLG
                     QRVKFRVEGEGDAARVTYVNILTLPDPE"
     gene            complement(2406..2837)
                     /locus_tag="VEP73_12430"
     CDS             complement(2406..2837)
                     /locus_tag="VEP73_12430"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYT27279.1"
                     /translation="MNRKELVDAIQKHTEVARADVDKVLASLIQHTQVAVKKGERVSL
                     VGFGTFERRDRKARTARNPRTGTAVKVKATKIPAFRAGQSFKDVVSGKASAPKLTAPK
                     ASARAGSGTRATKAAKATAAASKSRSTASAKTPAKAGSRKR"
     gene            complement(3359..4315)
                     /gene="ligD"
                     /locus_tag="VEP73_12435"
     CDS             complement(3359..4315)
                     /gene="ligD"
                     /locus_tag="VEP73_12435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYT27280.1"
                     /translation="MKNGPIELDGRTIEVSSAGKVFFPDAGVTKGDLVAYYRDVAGVT
                     VPHLRDRPLTLQRFPDGLGGQGFFQQDASDHFPAWVRRVPVERRGKGGTVGHVVADDA
                     ATLVYLANQAVVTFHAWTSRAGALDRPDLVVFDLDPDAGQGLDVVRAAARAVRAAVEG
                     VGLSPFVQTSGSKGFHVLAPLDGGADGEAVKAFARDLAALVAAHDPGRLTVEWRKAKR
                     AGRLLIDTNRNGYAQTVVAPYSVRARPGAPVATPIDWHELGRAEPRSWTVANLRRRLA
                     RKPDPWAGMEQHARPFAPARERLDELLAPAPGARPAASRRRR"
     gene            4563..5084
                     /locus_tag="VEP73_12440"
     CDS             4563..5084
                     /locus_tag="VEP73_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27281.1"
                     /translation="MAVVEKRISDLSGTMSDDMGRLTVLSHPKFSGPIQLDVLDDEIR
                     DMGGLGDIVEVEVTSSTGQTRKLILSAGDFNKLASDMEAVLTEAVARTASHRTVTTRK
                     RGRGRPARQDKSEKVDYAAPEHAGFPHRGRVSPEEAAYVREHFDEVQRRLSEAGVRVL
                     DPADPKTKERYGL"
     gene            5158..>5625
                     /locus_tag="VEP73_12445"
     CDS             5158..>5625
                     /locus_tag="VEP73_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYT27282.1"
                     /translation="MLLVEVAETSDAVASTAARLAKVDRLAACLRQLDANEVELAVAF
                     LSGELRQRQIGVGYAALRDAPAPAEHPSLTLVEVDAAFERIGRQAGPGSQRERRRLLA
                     DLFGRATATEQAFLRRLLSGELRQGALEGVMVEAVARAAGVPGTEVRRALMLRG"
BASE COUNT          724 a         2108 c         2041 g          752 t
ORIGIN      
        1 gtccttcctc tcaggtccct ggcgcgggct cgccgggccc gtgccctccc ccgccgtccg
       61 ggccggcgcc gggcagccta tcagcgcccg ccggcccgcg ggccgccgtc gtggcccgcg
      121 cgagcacccg ggcccggaag cgcctgccct gctcgccgcc tcggacgccc cggacccgcc
      181 gggtgcggcc gtcacgggtc accagccggg tgcggccgcc gccgatctcc acggccgaca
      241 gctccgcgta gcgccagcgc cgcatgcccg ccgcaccgtg cagcacgacg gcctcgtcgt
      301 cgagctcggc ctcccagacc cgcgcccgga gcgcacccgt ggcgaggctg tagacgccga
      361 gcaccgccca cagcacggcg gcggccacga gcgaggcggc cacgtccccg ttcgcctcgc
      421 cggcgagctg ggacaggccg aggaggacca gggcgagcgc cagctcgagc aggccgctgg
      481 cgaccgcgtg gagcaccccg ccgggacgga agcgctcgac cgccgcgggc tcggcgggct
      541 ctctgcggga cgatggcatg gtgtcttcgg ggatgtccgt ccggtcggtc gtcagggcgg
      601 cggcaggctc tggcctgcct cccgccgacg gtaccaggcc ggagggcgcc accccgcccg
      661 tgcctcgtcg accagggcgc cgatcgccgc catgacccgg tcgcgggccg cgttcacggc
      721 ctccagcggc gcatccggcg cgagcccgag gtccatcggc gggccggccc ggatgacgac
      781 cctgatgccg cgccggaacg gccccagctt gccgcgcgtg aacaacaggt gggcgcccca
      841 catcccgacc gggacgaccg gggcgccggt gagggcggcg agccgggcga cgccgttctt
      901 cgccggcatc ggcgtgaagt cggcgttgcg ggtcgtcgtg ccctccgggt agatggccac
      961 cgcggccccc tcctgcatgg cggcgaccgc cgccgcgagg gacttcgcgg cgtcgcggct
     1021 ctcccggtac acggggatct ggccggcccc gcgcaggatc cagcgcagca gccggccctc
     1081 gaacagctcg gacttggcca ggaagcgcac ctggcgccct gccgagtcga tgaaggtgcc
     1141 gaggcacagc gggtcgaagt aggagacgtg gttggcggcg atgatgaccg gcccgcgcgg
     1201 cgggatgtcg agggagcccc gcatgtccag ccggacccag aaccggatca ccggcgggac
     1261 cagggccttg gcaagggcgt agacgggcag tcgccagctt ggcctcatcg tcggatcgta
     1321 ccgtgccgcg agcctcggga tgcatcgccg cggcatcgcg cgggttgcag cttcggcacc
     1381 accgcccggc accaccgccc ggccggctgc tgctatcgtc ggccgcgtgc cgaccccgct
     1441 cctgcccgtg aaggccctcg ccgacgcgaa gggccggctc gggccttccc tcacgccgat
     1501 cgagcgccgc ctgctcacca tcgcgatgct cgaggacgtg gtcgcggcgc tccaggccgt
     1561 gcccggcctc ggccggccgg tcgtcgtctc ccccgaccgg gaggtctggc gccgcgcgga
     1621 cgccatcggc tgccgggtgg tggaggaggt cgggaccccc gccggcctca acgcctcgct
     1681 ggccacggcc gcggtggcgg cccgggccgg cgacggcgcc ggggacgggc tcgtggtggt
     1741 cgccgcggac ctgccgctgg ccactcccgg cgccatctcg cgcgtcctcg aagccgccgc
     1801 ggcggcaccc gtcgtggtgg tgcccgaccg cgacggcggc ggcaccaacg tcctcgcctg
     1861 gcgcggcccg gcgccgttcc gccccgcgtt cggcccggac agcgcgaggc gccacctcgc
     1921 cctccccggt gccgtccggc tcgacgagcc ggccctcgcc ctcgacctcg acaccgtcga
     1981 cgacctgcgc gccgtggccg ccggcgtcga ccccgccagc gtcaccggcc gccgcctgcg
     2041 ggacctgcgc ctgccggccc ggctcgagcg ggcgggctga ccggacgaac cagctggacg
     2101 gacaaaggag tggcccgatg ctgcagggga ccgtcaagag cttcgacccg aagaccaggt
     2161 cggccgttgt cctcaccgac gcgagggtcg agtacgccgt ccccccggag gcgttcggcc
     2221 gttcgggcct gcgcctgctg tcgctcgggc agcgggtcaa gttccgggtc gagggagaag
     2281 gcgacgccgc cagggtcacc tacgtcaaca tcctcacgct ccccgacccc gagtagtccc
     2341 gcccggtccg tactgcgcga cgaggccccg cggacggcgg ggcctcgtcg cgccgtggcc
     2401 gccggctagc gcttgcgcga cccggccttg gccggggtct tcgcgctcgc cgtgctccgg
     2461 ctcttcgagg cggcggccgt cgccttggcc gctttcgtgg ccctggtgcc gctgccggcg
     2521 cgggcgctcg ccttcggggc cgtcagcttg ggggcggacg ccttcccgct caccacgtcc
     2581 ttgaagctct gcccggcccg gaaggcggga atcttcgtcg ccttcacctt gaccgcggtg
     2641 ccggtgcgcg ggttgcgcgc ggtgcgagcc ttgcggtcac ggcgctcgaa ggtgccgaac
     2701 cccaccagcg acacccgctc gcccttcttc acggcgacct gcgtgtgctg gatgaggctc
     2761 gcgagcacct tgtcgacgtc agcccgggcc acctccgtgt gcttctggat ggcgtcgacc
     2821 aactctttgc ggttcaaggg cgcctccttg cccaataggg gattgctccc cacccaggac
     2881 ggacccccgt agccctgtcc cctttcggtc gtgcatgcta aagacgcggt tatccgaggt
     2941 caacaaccct ccgcgtttcc aatcccttgc ggggcaacgg gtatggccgc gaacccgccg
     3001 cggcgggccc gtcgcgccga aatggagggc cgtgccgtgc cgcaattcat gaggccgtcg
     3061 aacgcgtctt gcctgcacag gcaccatcca tcccttgccg gggcgggcgc acagtcgggc
     3121 tcgcgccgcg cttgccggcg cgcggatttc ggcatcgcaa gcctcccggg ccacccaaag
     3181 ccccgccgca aaggcacccc caatgggagg tatcgggcaa gcgcggccgg ggtcgaatcg
     3241 gcgccgccgg aaaggggagg gccacgcccg gccccgagcg ggcaaacagg tgctctggca
     3301 ggggcaggcg cccggacgac ccgccgcacc gcgccgcgac cgccgcggcc gctcggcgtc
     3361 accgcctccg tcgggacgcc gccggccggg cgcccggcgc aggcgcgagc agctcgtcga
     3421 ggcgctcccg ggcaggagcg aacgggcggg cgtgctgctc catgccggcc caggggtccg
     3481 gcttgcgcgc cagccggcgg cgcaggttcg cgaccgtcca ggagcgcggc tcggcacggc
     3541 cgagctcgtg ccagtcgatc ggggtggcga ccggggcgcc gggacgggcg cgcaccgagt
     3601 acggggcgac caccgtctgg gcgtacccgt tgcggttggt gtcgatgagc aggcggccgg
     3661 cccgcttggc cttgcgccac tcgaccgtga gccggccggg gtcgtgcgct gcgacgaggg
     3721 cggcgaggtc ccgcgcgaac gccttcaccg cctccccgtc cgcgccgccg tcgagcgggg
     3781 ccagcacgtg gaaccccttg gagccgctcg tctggacgaa gggcgagagc cccaccccct
     3841 cgaccgccgc gcggacggcg cgggcggccg cgcggaccac gtcgagcccc tggccggcgt
     3901 cgggatcgag gtcgaacacg acgaggtcgg ggcggtcgag ggcgccggcc ctggaggtcc
     3961 aggcgtggaa cgtcaccacc gcctggttgg ccaggtacac gagcgtcgcg gcgtcgtcgg
     4021 cgacgacgtg gcccaccgtg ccgcccttgc cgcggcgctc caccggcacc ctccggaccc
     4081 acgccgggaa gtggtccgag gcgtcctgct ggaagaagcc ctggccgccg agcccgtccg
     4141 ggaagcgctg cagggtgagg ggacggtccc gcaggtgcgg gaccgtgaca ccggccacgt
     4201 cacggtagta ggcgaccagg tcgcccttgg tgacgccggc gtccgggaag aagaccttgc
     4261 cggcgctcga gacctcgatg gtgcggccgt cgagctcgat cggcccgttc ttcatgctcc
     4321 ccgcccttcc tgggctgccc ggcccggtcg tcgtccccgc acgtcacaat atgccaaggc
     4381 aacaagtctg atcgtgaggc tgtgaggtgc ggcttgtagc tccgttccgt tctgctcact
     4441 ttcacggccc acgcctggac gacgcgggcc aaccgctttt ctcggcagcc cggtaggtgc
     4501 gcccgcctgc cgtaatcccg gtggtaaact ttgccaaacc cgcaaagcga gggaggcggc
     4561 agatggccgt agtcgagaag cgcataagcg acctgagcgg caccatgagc gacgacatgg
     4621 gccgtttgac ggtcttgagc caccctaagt tttccggtcc catccagctc gacgtgctgg
     4681 acgacgagat cagggacatg ggtgggcttg gcgacatcgt cgaggtggag gtgacaagct
     4741 ccaccggaca gacccgcaag ctcatcctct cggccgggga cttcaacaag ttggcctccg
     4801 acatggaggc cgtcttgacc gaggccgtag cgcgcacggc gtcccaccgc accgtcacga
     4861 cacgcaagcg cgggcgcggg cgcccggcca ggcaggacaa gagcgagaag gtcgactacg
     4921 ccgctcccga gcacgccggt ttcccgcacc gtgggcgggt cagccctgag gaagcggcct
     4981 atgtccgtga gcacttcgac gaggtgcaga ggcgcctgtc cgaggccgga gtgcgggtgc
     5041 tcgatcccgc cgaccccaag accaaggagc gctacggcct ctgagccgct cctgccaggg
     5101 gaatccgcca gacgggcggt tttgacgaca tgaacaatat cggtccgcat tagggccatg
     5161 ctgctggttg aggttgccga gacgtccgac gccgtggcgt cgaccgctgc ccggctggcc
     5221 aaggtggacc ggctggccgc ctgcttgcgc cagctcgatg ccaacgaggt ggaactagcc
     5281 gtagcgttcc tctccggcga gctacggcag cggcagatcg gggtcggtta tgcagcgctg
     5341 cgagacgccc cggcgcctgc tgagcacccc tccctcacgc ttgtcgaggt tgatgccgcg
     5401 tttgaacgga tcgggcggca ggccgggccg ggctcccagc gcgagcggcg ccggctgctc
     5461 gctgatctgt tcgggcgagc gaccgccacc gagcaagcgt tcctccgccg gctgctctcc
     5521 ggtgagctgc gccagggtgc gctcgagggc gtcatggtcg aggctgtcgc acgggcggcc
     5581 ggtgtcccgg gcactgaggt tcggcgcgcc ctgatgctcc ggggc
//
LOCUS       DASPYY010000797         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_838650, whole genome shotgun sequence.
ACCESSION   DASPYY010000797 DASPYY010000000
VERSION     DASPYY010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838650"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1345)
                     /locus_tag="VEP73_12450"
     CDS             complement(161..1345)
                     /locus_tag="VEP73_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HYT27283.1"
                     /translation="MLQDPARPAIPAPPVPRYGEASLSELLPSALAALDVPGCRNPLG
                     LEPARRACLLVVDGLGWELLQANRERAPFLAAAAEGGSPLTAGYPSTTASSLASIGTG
                     RPPAEHGLVGYTIALPGMDRAFNCLRWSPYGLGGSKDLRGRVVPERIQPEPTAFERAA
                     ADGIQVSLLGPAEHAGSGFTRAVLRGGDYAGVLSLGDQAAAALHRLAGSRRSLVYAYH
                     PDLDRTGHVRGIGTDAWRLQLAHVDRLAAHLAERLPHDAVLLVTGDHGMVDLRPEQRI
                     DLADQPELAGGVRLLAGEARARHVHVAEGAAGDVLAAWREVLGDSMWVVSRDEAIGAG
                     WFGPHVPDRVRPRIGDVVAAAFGPVGIVQRDVDPSQARLTGHHGSMTPAEQLVPLVEI
                     RR"
     gene            1487..1717
                     /locus_tag="VEP73_12455"
                     /pseudo
     CDS             1487..1717
                     /locus_tag="VEP73_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            1833..>2439
                     /locus_tag="VEP73_12460"
     CDS             1833..>2439
                     /locus_tag="VEP73_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27284.1"
                     /translation="MAEERQDLPTYNVLKSWAFDDWGLYGRGDELLSRALARHARVGR
                     VFHVQPPVDPAELRTRFRDPRWEQDMAGQLRRFDGVDDGGVFLFTPTADPARQDAALD
                     RTVEMLEREQAFARPLIMLHGLTHPSSVDLAQRLGGRGVTRVAQVRRDARAAYPPDAD
                     ERQALDDIYRAQVPRADVVWAGTSELVEELAALNSRPVLHPV"
BASE COUNT          310 a          909 c          957 g          263 t
ORIGIN      
        1 gcgctcgccg ccgccgggct gatcgggctc cgccagcgcg acatccccgt cacctgaccg
       61 accacacccg gtgccgcgga gctcgaacct ccgcctgcac gaggggcgtc cgggccgtct
      121 cggcgcacgg cccgcgggtg agcgtcgtga tccagggcgg ctaccggcgg atctcgacca
      181 gggggacgag ctgctcggcg ggtgtcatcg agccgtggtg gccggtgagc cgcgcctggg
      241 aggggtcgac gtcgcgctgc acgatcccga caggcccgaa cgcggccgcg accacgtcgc
      301 cgatgcgggg acggacccgg tcgggcacgt gcggcccgaa ccatcccgcc ccgatcgcct
      361 cgtcgcggga cacgacccac atcgagtcgc ccaggacctc ccgccaggcg gcgagcacgt
      421 cgccggccgc cccctcggcg acgtggacgt ggcgggcgcg ggcctccccg gcgagcagcc
      481 gtaccccgcc ggccagctcc ggctggtcgg ccaggtcgat ccgctgctcg ggccggaggt
      541 cgaccatgcc gtggtcaccg gtcacgagca ggacggcgtc gtgcggcagg cgctcggcca
      601 ggtgcgccgc cagccggtcg acgtgggcga gctgcaggcg ccaggcgtcg gttccgatgc
      661 ccctgacgtg gccggtgcgg tccaggtcgg gatggtaggc gtagaccagg ctgcgccggg
      721 agccggccag gcggtgcagg gcggcggcgg cctggtcgcc gagcgacagc acgccggcgt
      781 agtccccgcc gcgcaggacc gcccgggtga acccggagcc ggcgtgctcg gccgggccga
      841 gcaggctgac ctggatcccg tcggccgcgg cgcgttcgaa ggcggtcggc tcgggctgga
      901 tccgctcggg gacgacccgg ccgcgcaggt ccttggaccc gccgaggccg tagggggacc
      961 agcgcaggca gttgaaggcc cggtccatcc cgggcagcgc gatcgtgtag cccacgaggc
     1021 cgtgctcggc cggcgggcgc ccggtgccga tcgacgcgag gctcgacgcc gtggtcgagg
     1081 gatagccggc ggtgagcggc gacccgccct cggccgcggc ggccaggaac ggcgcccgct
     1141 cccggttcgc ctgcaggagc tcccagccga gcccgtcgac cacgagcagg cacgcccggc
     1201 gggccggctc gagcccgagg gggttgcgac agcccggcac gtccagggcg gcgagcgccg
     1261 aaggcaggag ctcggacagg gacgcctcgc cgtagcgggg gacgggcggg gccgggatgg
     1321 cgggcctggc tgggtcttgc agcatggcgc gagagtagca agggcgcgag cccgagggca
     1381 gggacgcacg agacccacac cggtgcttga cttccccggg cggccgggcc gaaggtagcg
     1441 cccgacccga cccgaccagg ccggccaggc cgagcaggag ccgccagtgg accgccacga
     1501 gttcgacccc ggctacgccg acgagccgtt ccgctccctg tgcgtggagt acccgggcgg
     1561 ggagacctac ccgccggctg acttccgggt ggagtgggga ccggtcttcc accggggccg
     1621 gctcgacggc agcgcgcgcg tgctcgtgct cggccaggac cccgcgcagc acgagacggt
     1681 cgcgcgccgc atcctcgtgg gcgcggccgg gcagcgcccc tggaagcagg gctgacccgc
     1741 ccgcccgccc ggccggttcg gggccggcct gcgcgccgtc gtaggatgcg ccggcacaca
     1801 gtcgaccgac gagcgatggt ggtggagggc gcgtggccga ggagaggcag gacctgccga
     1861 cctacaacgt gctcaagagc tgggcgttcg acgactgggg cctctacggg cgaggcgacg
     1921 agctgctcag ccgggcgctt gcccgccacg cccgggtggg ccgcgtcttc cacgtgcagc
     1981 cgccggtcga cccggcggag ctgcgcaccc gtttccgcga cccgcgctgg gagcaggaca
     2041 tggccggcca gctccgccgg ttcgacggcg tcgacgacgg cggcgtgttc ctgttcaccc
     2101 cgaccgccga cccggcgcgg caggacgccg ccctcgaccg caccgtcgag atgctcgagc
     2161 gggagcaggc cttcgcccgc ccgctgatca tgctgcacgg gctgacccac ccgtcctcgg
     2221 tcgacctcgc gcagcggctc ggggggcgcg gggtgaccag ggtggcgcag gtccgccggg
     2281 acgccagggc cgcctacccc ccggacgccg acgagcgcca ggccctcgac gacatctacc
     2341 gggcccaggt cccgcgagcc gacgtcgtct gggcggggac gagcgagctc gtcgaggagc
     2401 tcgcggcgct gaactcccgg cccgtgctgc acccggtcg
//
LOCUS       DASPYY010000798         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1118454, whole genome shotgun sequence.
ACCESSION   DASPYY010000798 DASPYY010000000
VERSION     DASPYY010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118454"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..757
                     /locus_tag="VEP73_12465"
     CDS             164..757
                     /locus_tag="VEP73_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27285.1"
                     /translation="MRLRVVTAGAGAAWEAALAQACQQSGAPAEIVQRCYDLGDLLAV
                     AAAGQAQVAIVASATRWLDREAVTRLGASGVAVVGVVPAGDEDAERRLRQLGIVHVAR
                     DADRPGELVELARAASRTEPRDDDPPTPAAPDGAPRRQHALVAVWGPKGAPGRSTVAL
                     NLAFEAAQLAGETLLVDADTYGGSIAQTTMQFSLGAC"
     gene            832..1101
                     /locus_tag="VEP73_12470"
     CDS             832..1101
                     /locus_tag="VEP73_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27286.1"
                     /translation="MRTWETNTPDGSRPIAAPLERCAELLGVDLALVRRAAANVEPYV
                     RADGTKVWSLMQLERELRPEVFVRRWAGGYLTRRRARAADQRTRR"
BASE COUNT          221 a          581 c          511 g          212 t
ORIGIN      
        1 caggtcagga tgccgggcga gcgggcgacc ctggtcacgg ccgccatcgc caccgcccgc
       61 gtcttcgtcg tcaaggcgcc ggccaggccc gcacccgcgc cgtccgcggc cgtcccgccc
      121 ccacccgcca gcctcccggc cagctcgtcg ccgtgacccc gacatgagac tgcgcgtggt
      181 caccgccggc gccggcgcgg cctgggaggc ggcgctcgcg caggcctgcc agcagagcgg
      241 cgccccggcc gagatcgtcc agcgctgcta cgacctcggc gacctcctcg cggtcgccgc
      301 cgccggacag gcccaggtcg ccatcgtcgc gtcagccacc cgctggctgg accgggaggc
      361 ggtcacccgc ctgggcgcgt ccggcgtcgc cgtcgtcggg gtcgtccccg ccggcgacga
      421 ggacgccgaa cgccggctgc gccagctcgg catcgtccac gtcgccaggg acgccgaccg
      481 gcccggcgag ctcgtcgagc tcgcccgcgc cgcgagccgg accgagccgc gcgacgacga
      541 ccctcccacc ccggccgcac ccgacggggc gccccgtcgc cagcatgcgc tcgtggcggt
      601 ctggggaccc aagggggcgc ccggacgctc gacggtcgcg ctcaacctcg ccttcgaggc
      661 ggcccagctg gccggcgaga ccctgctggt cgacgccgac acctacggcg gctcgatcgc
      721 ccagaccacc atgcaatttt ccttaggagc atgctgaggt tgagggccta gacgcgtcgc
      781 tcgacgtcga taccgttcac ttcgaacaag cgttcgacta tgcttccggg catgcgcacc
      841 tgggaaacga acacccccga cggcagcagg ccaatcgcgg ccccgcttga gcggtgcgcc
      901 gagctgctcg gtgtcgacct ggccctcgtc cgcagggcgg ccgccaacgt cgagccctac
      961 gttcgagcgg acggcacgaa ggtctggagt ctcatgcagc tcgagcgaga actccgccct
     1021 gaggtgttcg tccgccgttg ggccggcggg tacctcaccc gccggcgcgc ccgcgccgcg
     1081 gaccagagaa caagacgctg aagcgctgag cgcggctgga ctggaccgca aacggagccg
     1141 caaggcatga agctccgtcg acgcgaagcg cttggatcgc gctggcgatc gtggatcgtt
     1201 cgggctcgtg cccctgttct tgcccgtggt gcgccgtcag cggcagcacc ggccgcgcgg
     1261 gtagctgacc tcttgcatgc cacatacggc aatgccgcca cggacgctgt ccgctcggaa
     1321 ccgccattac tggcctcggc gttccgtacc taccgttggt gaccgctgtt gtccgttggg
     1381 taccgcgcgc atgcggcccc agcgcggccc cactagccgg aagttacgtg agccatgagg
     1441 gccgaaaacg accatctacc tgcagaaaca ctgctcagat gagtccaggg tgtagccaga
     1501 aagtgttgct tcctggctcg attct
//
LOCUS       DASPYY010000799         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2101719, whole genome shotgun sequence.
ACCESSION   DASPYY010000799 DASPYY010000000
VERSION     DASPYY010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101719"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..743
                     /locus_tag="VEP73_12475"
     CDS             159..743
                     /locus_tag="VEP73_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27287.1"
                     /translation="MSTSGHPGATGGRVRPGGRRWVPPVALLAFLAVLVGGGYVTQNA
                     LANEVEPPVRVGPGVVVHPLSGWRQASTRNDAVLLTRGSGNLAVLEVDGQADPAELAA
                     WYVATRLRPAASGLVLSRPLPAVRLPSGLVGARFLYQGDFHAGERSMPLRGEVTTVTG
                     PAGRGAVFDAWALPETFRYVLGDVHRMIDTAEVR"
     gene            745..1965
                     /locus_tag="VEP73_12480"
     CDS             745..1965
                     /locus_tag="VEP73_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HYT27288.1"
                     /translation="MGARAVRAPVAQRVRPPRWGSTTTLFQFREPAFWVYVLLLAVTG
                     VRFVQEQAQLRGLSPSGWVLSWVLLALYLLPMFAVIYLLDIYEREPLSLVVGALLWGA
                     VCATTLAGLGNDGWIGVVARLGGPDFAATWSAALSAPLVEESVKAAGVVLIYLIATAE
                     IDDELDGFVYGAMVGLGFAVVENVLYFVGSFGGTPGGVLSGFFVRVLASGLYGHVLYT
                     GLAGMGIAYLLTRRGEVSAARRALVATALCLLAVLAHFLWDSPLLDLFPHGNVSLARL
                     LVQVPLAAAVKGVPFLAVLVVMILLAQRRERRWLEAALEGELGGPGLAAGELEILADP
                     RRRRRVRRVVRKRDGRRAAALVKQLQREQVTLAMLRTRVDADDHPDLVRQRRRIAAIC
                     EALAAEMAPAPQRR"
BASE COUNT          219 a          753 c          786 g          319 t
ORIGIN      
        1 taagattgcg ctgtgtgttt cctgagtgcc gggtgcggcc gctcgccggg atccggcgcg
       61 ggcggccggc ggcgtcggcg ttcagggagc gcgctccccg acaacccatg gttcccgccg
      121 gacgctgagg aaggggcgat cacgtgcggc accccggcgt gagcacgagc ggccacccgg
      181 gcgcgaccgg cggccgcgtg cggccgggag ggcggcgctg ggtgcccccg gtcgccctgc
      241 tcgccttcct cgcggtcctc gtgggcggcg ggtacgtcac ccagaacgcg ctcgcgaacg
      301 aggtggagcc gcccgtccgg gtcgggccgg gggtcgtggt ccacccgctc tcggggtggc
      361 ggcaggccag cacccgcaac gacgccgtcc tgctcacgag gggcagcggc aacctcgccg
      421 tgctcgaggt cgacggccag gccgatcccg ccgagctggc cgcctggtac gtggcgaccc
      481 ggctgcggcc ggccgcgagc gggctggtcc tctcgaggcc gctgcccgcc gtcaggctgc
      541 cctccggcct cgtcggcgcg cgcttcctct accagggcga cttccacgcc ggcgagcggt
      601 cgatgcccct gcggggcgag gtcaccacgg tgaccggccc ggcgggcagg ggcgcggtct
      661 tcgacgcctg ggcgctgccg gagaccttcc ggtacgtcct gggcgacgtg caccggatga
      721 tcgacaccgc ggaggtgcgc tgagatgggc gcccgtgccg tccgcgcacc ggtcgcgcag
      781 cgggtgcggc caccgcgctg gggctcgacg acgaccctgt tccagttccg cgagcccgcc
      841 ttctgggtgt acgtcctgct gctcgcggtg accggcgtcc gcttcgtgca ggagcaggcc
      901 cagctgcggg gcctctcccc gtccggctgg gtgctgtcct gggtcctgct cgccctctac
      961 ctgctgccga tgttcgccgt catctacctg ctggacatct acgagcgcga gccgctctcg
     1021 ctggtggtcg gggcgcttct gtggggggcg gtctgcgcga ccacgctggc cgggctcggg
     1081 aacgacggct ggatcggcgt cgtcgcccgg ctcggcgggc ccgacttcgc cgcaacctgg
     1141 agcgcggcgc tcagcgcacc gctggtcgag gagagcgtga aggccgccgg ggtggtgctc
     1201 atctacctga tcgccaccgc ggagatcgac gacgagctgg acggcttcgt gtacggcgcc
     1261 atggtcgggc tcgggttcgc ggtcgtggag aacgtcctct acttcgtggg ctcgttcggg
     1321 ggcaccccgg ggggcgtcct cagcgggttc ttcgtccgcg tgctggccag cggcctctac
     1381 ggccacgtcc tctacaccgg gctcgccggc atgggcatcg cctacctttt gacccggcgc
     1441 ggcgaggtga gcgcggcgcg gcgcgcgctc gtcgccaccg cgctctgcct gctcgcggtc
     1501 ctcgcccact tcctgtggga ctcgccgctg ctcgacctgt tcccccacgg gaacgtgtcg
     1561 ctcgcccgcc tcctcgtcca ggtgccgctc gcggccgcgg tcaagggcgt gccgttcctc
     1621 gcggtcctcg tcgtcatgat cctgctcgcg cagcggcggg agcgccgctg gctcgaggcg
     1681 gcgctcgagg gtgagctcgg ggggccaggg ctcgcagcag gcgagctcga gatcctcgcg
     1741 gacccgcgcc ggcgccgccg ggtgcgccgg gtggtgcgca agcgggacgg ccggcgggcc
     1801 gccgccctgg tgaagcagct ccagcgcgag caggtcaccc tcgcgatgct gcggacccgg
     1861 gtcgacgccg acgaccaccc cgacctggtc cgccagcgcc ggcgcatcgc ggcgatctgc
     1921 gaggccctcg cggccgagat ggcacccgcg ccgcagcgtc gctagctact tccccatgaa
     1981 gaagtcggcg gcggcgtagc cgctgagctg cgggaatgct aggacgagcg gcgtggttgt
     2041 ggctgaatga caccgttgtc gctgttgtag gagatca
//
LOCUS       DASPYY010000800         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1821651, whole genome shotgun sequence.
ACCESSION   DASPYY010000800 DASPYY010000000
VERSION     DASPYY010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821651"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="rimM"
                     /locus_tag="VEP73_12485"
     CDS             <1..357
                     /gene="rimM"
                     /locus_tag="VEP73_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HYT27289.1"
                     /translation="SSRLHQGRLLLAFQGIDDRTAADSLRGRLLSIPARAARPLEPGE
                     YWPHQLVGLAVVDRDGEERGTLADVVPGTAHDLLSVRLHDGATALVPAVAALVTVDLD
                     AGRVVVDALPGLLDPS"
     gene            367..1113
                     /gene="trmD"
                     /locus_tag="VEP73_12490"
     CDS             367..1113
                     /gene="trmD"
                     /locus_tag="VEP73_12490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYT27290.1"
                     /translation="MLFDVVTIFPGFFDSPLATGLLGRAGERGLVTVRCHDLRAWTHD
                     RHRTVDDAPFGGGAGMVMLPGPWFEALDALAGERGVPGPPHVVLLAPDGTRFDQAAAA
                     RLARMPRVVLCCGRYEGVDERVRTRVDEVLSIGDYVLAGGETAALAVVDAVARLVPGV
                     MGNAASAGEESFATPGGARLLEYPQYTRPASYRGLDVPAVLRSGDHGAIARWRRAQAA
                     ERTRRARPDLLARAEPAGSEPPDPAGPGRS"
     gene            1276..1623
                     /gene="rplS"
                     /locus_tag="VEP73_12495"
     CDS             1276..1623
                     /gene="rplS"
                     /locus_tag="VEP73_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYT27291.1"
                     /translation="MQITDVVERAQLRDDLPDFRPGDTVKVHARVVEAGRERIQVFQG
                     VVISRKGGGTRQMFTVRKISGGVGVERMFPLYSPTIDRIEVERHGRVRRAKLYYLRDL
                     RGKAARIEERRKG"
BASE COUNT          196 a          705 c          572 g          221 t
ORIGIN      
        1 agctcccgcc tccaccaggg ccgcctgctg ctcgcgttcc agggcatcga cgaccgcacc
       61 gccgccgact ccctgcgcgg gcgcctgctc tccatccccg cccgagccgc ccgccccctc
      121 gagcccggcg agtactggcc ccaccagctc gtcggcctcg ccgtcgtcga ccgcgacggc
      181 gaggagcgcg gcaccctcgc cgacgtcgtg ccgggcaccg cccacgacct gctgtcggtc
      241 cggctccacg acggcgcgac cgccctcgtg cccgcggtcg ccgcgctcgt caccgtcgac
      301 ctcgacgccg ggcgggtcgt cgtcgacgcc ctccccggcc tcctcgaccc ctcgtagggc
      361 gccgccgtgc tgttcgacgt cgtcaccatc ttccccgggt tcttcgactc gccgctggcc
      421 accggcctgc tcggcagggc gggcgagcgg ggtctcgtca ccgtccgctg ccacgacctg
      481 cgcgcctgga cccacgaccg ccaccgcacg gtcgacgacg cccccttcgg cggcggcgcc
      541 ggcatggtca tgctccctgg cccctggttc gaggccctcg acgccctggc cggcgagcgc
      601 ggcgtcccgg ggccgcccca cgtcgtcctc ctcgcgcccg acggcacccg attcgaccag
      661 gcggccgccg cccgcctcgc ccgcatgccc cgcgtcgtcc tgtgctgcgg ccggtacgag
      721 ggcgtcgacg agcgcgtccg cacccgcgtc gacgaggtcc tctccatcgg cgactacgtc
      781 ctggccggcg gcgagaccgc agcgctcgcc gtcgtcgacg ccgtcgcccg cctcgtgccc
      841 ggcgtcatgg gcaacgccgc ctcagccggg gaggagtcct tcgccacgcc gggcggtgcc
      901 cgcctgctcg agtaccccca gtacacccgc cccgcctcct accgcggcct cgacgtccca
      961 gcggtcctgc gctccggcga ccacggcgcc atcgcccgct ggcgccgggc gcaggcggcc
     1021 gagcgcaccc gccgcgcccg ccccgacctg ctcgcccgcg ccgagccggc cgggagcgaa
     1081 ccgcccgacc ccgccgggcc gggcaggagc tgaccccgcc ggcccgcccg gcgggcaggt
     1141 ggagcgcgac gaggggagcg tgttaccctc gcaggtagcc gcgagccggt tcgtgcgccg
     1201 gcacatgggc gcaccccgat ccgccggacc ggccaccctg cgtctcacgc cgttcccaag
     1261 ggagcttcac ccaacatgca gatcaccgac gtggtcgagc gcgcgcagct gcgcgacgac
     1321 ctccccgact tccgccccgg cgacaccgtc aaggtgcacg cgcgggtggt ggaggcggga
     1381 cgtgagcgca tccaggtgtt ccagggcgtc gtcatttcgc gcaagggggg cgggacgcgc
     1441 cagatgttta ccgtccgcaa gatctcgggc ggggtgggag tggagagaat gttcccgctc
     1501 tattcgccga cgatcgatcg tattgaggtg gaacgccatg gccgggtccg tcgcgcgaag
     1561 ctctactacc tccgggatct ccggggcaaa gcagcgcgca tcgaagagcg ccgcaagggc
     1621 tgaccggccc ggccggttgc gtgacttgcc cgaggccgcc gcacgagcgt cgtcttcgga
     1681 gcggtctgtc gcga
//
LOCUS       DASPYY010000801         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1821658, whole genome shotgun sequence.
ACCESSION   DASPYY010000801 DASPYY010000000
VERSION     DASPYY010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821658"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEP73_12500"
     CDS             <1..366
                     /locus_tag="VEP73_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HYT27292.1"
                     /translation="AASEGDKTMVDALAPAVAAFDVELAAGGSLESAVRRASAAATGG
                     MRATIPLQARKGRASYLGPRSVGHQDPGATSTAVVLGALERVVLGAGAGARGPSRSPP
                     AAQRDTPSWFPGDPDHHVP"
     gene            356..1198
                     /locus_tag="VEP73_12505"
     CDS             356..1198
                     /locus_tag="VEP73_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT27293.1"
                     /translation="MSLEPPRPMAGRHPARRDDERTLIQSVSRAARILKALGSETSRL
                     GVTELAARLDLKKATVHGLLRTLEAHQLVEQDTETGKYRLGPALLQLGNAFLDNHELR
                     ARSLLWAESLATRAQEAVHVGVLTGPSVLIVHHVFRPDNSVQILEVGASIPWHACALG
                     KAIVAHLGPGEQRELLSGELPRLTGRTATDPAALDRELVRVRREGTAFERQEAIVGEA
                     GIAAPLFGSRGTVVGAIGLSGPVEHLLPDGPPAATVTAVKEVARGLSRELGGGRAAAP
                     GVGA"
     gene            complement(1206..1580)
                     /locus_tag="VEP73_12510"
     CDS             complement(1206..1580)
                     /locus_tag="VEP73_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HYT27294.1"
                     /translation="MANLVVHLVTPEREVWTGEASFVIARGVDGDLGVLPGHAPLLAA
                     LAVGPVFIEADGGFLHIASPEEGVTRVDVLAEHALLADELDREDPAVCEQRAQELEAE
                     GRFDEARVETAKALTRRRLAEG"
     gene            complement(1583..3022)
                     /gene="atpD"
                     /locus_tag="VEP73_12515"
     CDS             complement(1583..3022)
                     /gene="atpD"
                     /locus_tag="VEP73_12515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYT27295.1"
                     /translation="MATVAVGQDTQQGTGRIVRVIGPVIDVEFAPDEIPAIFNAVTVE
                     RTIGEQTTVITCEVAQHLGDNTVRAIAMQPTDGLVRGAPVTDTGESMTVPVGEKTTGH
                     VFNVLGQALDDPDFEADVRWPIHRHSPPYDKLESKTEMFQTGIKVIDLLEPYVQGGKI
                     GMFGGAGVGKTVVIQEMIHRVAEEHSGVSVFAGVGERTREGNDLFREMTESGVIEKTA
                     LMFGQMDEPPGVRLRVGLAALTMAEYFRDEMGQDVLLFIDNIFRFVQAGSEVSTLLGR
                     MPSAVGYQPTLADEMGELQERITSAGGHSITSLQAIYVPADDITDPAPHTTFAHLDAT
                     TVLSREISQLGIYPAVDPLASTSRILDPRYVGDEHYGVARRVQQILQRNNDLQDIIAI
                     LGVDELSEEDRVTVNRARRIQRFLSQPFYVAERFTGVKGKTVPLEETIASFQALADGE
                     YDHLPEQAFFNVGGIEEAEEKAKQIEGEG"
     gene            complement(3030..3917)
                     /locus_tag="VEP73_12520"
     CDS             complement(3030..3917)
                     /locus_tag="VEP73_12520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HYT27296.1"
                     /translation="MAAQLRVVRRRIRSVQSTMKITRAQQLISASRVARAQQRVQQAR
                     PYTEEVTRALTSVANTEITISHPLLEQRAEVSAAAVLVVTSDRGLAGPYNANVLRTTE
                     ELLARLRQEETEPRLYVVGRKGLAYYRFRQRPVAESWTGFSDRPSYEDAKRVADTMLE
                     AFLDHEVDEIHAVFTDYVSAMTQRSTATRFIPLVVEETRERPEQPIPMYIYEPDAQAV
                     LDALLPRYVEARVFTAMLEAAASEHASRQKAMSAATDNAEKLIEKLTREYNQARQAEI
                     TQEIMEIVGGAEALQKTGS"
     gene            complement(4065..5606)
                     /gene="atpA"
                     /locus_tag="VEP73_12525"
                     /pseudo
     CDS             complement(4065..5606)
                     /gene="atpA"
                     /locus_tag="VEP73_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(5690..>5876)
                     /locus_tag="VEP73_12530"
     CDS             complement(5690..>5876)
                     /locus_tag="VEP73_12530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HYT27297.1"
                     /translation="DGERRARLAEVLTRMVGKPVELRCTVDPAILGSVVVRIGDELLD
                     GSVRTRLELAREALGVA"
BASE COUNT          742 a         2061 c         2165 g          908 t
ORIGIN      
        1 gcggcctccg agggggacaa gaccatggtg gacgcgctcg ccccggcggt cgccgccttc
       61 gacgtggagc tcgcggccgg cggctccctc gagtccgcgg tcaggcgggc gagcgccgcc
      121 gcgaccggcg ggatgcgggc gaccatcccc ctgcaggcca ggaaggggcg ggcgagctac
      181 ctcggcccga ggagcgtcgg gcaccaggac ccgggggcga cctccaccgc ggtcgtcctc
      241 ggcgcgctcg agcgggtcgt cctcggcgcg ggcgccggcg cccgggggcc gtcgaggtcg
      301 ccgcccgcag cccagaggga caccccttcg tggttccctg gggacccgga ccaccatgtc
      361 ccttgagccc ccgcgcccca tggcgggcag gcacccggcg cggcgcgatg acgagaggac
      421 cctgatccag tcggtgagcc gggcggcgcg catcctcaag gcgctcggct cggagacgtc
      481 ccgcctcggg gtcaccgagc tggcggcccg gctggacctc aagaaggcga ccgtccacgg
      541 gctgctgcgc accctcgagg cccaccagct cgtcgagcag gacaccgaga ccggcaagta
      601 ccgcctcggg ccggccctgc tgcagctcgg caacgccttc ctcgacaacc acgagctcag
      661 ggcgcgctcg ctgctgtggg ccgagtcgct cgccaccagg gcgcaggagg cggtgcacgt
      721 cggggtcctc accggcccga gcgtcctgat cgtccaccac gtcttccggc cggacaactc
      781 ggtgcagatc ctcgaggttg gcgcctcgat cccgtggcac gcctgcgcgc tcggcaaggc
      841 catcgtcgcc cacctcggcc ccggcgaaca gcgggagctt ctctccggcg agctccccag
      901 gctgaccggg cggacggcca ccgacccggc ggcgctcgac cgggagctcg tccgcgtccg
      961 gcgcgagggc accgccttcg agcggcagga ggcgatcgtc ggcgaggcgg ggatcgccgc
     1021 cccgctgttc ggcagccgcg gcacggtcgt cggcgccatc ggcctgtccg ggccggtcga
     1081 gcacctgctg cccgacggcc cgccggccgc caccgtcacc gccgtgaagg aggtcgcccg
     1141 tgggctgtcc cgcgagctcg ggggcggccg cgcggccgcc cccggcgtcg gcgcctgacc
     1201 ccgcgtcagc cctcggcaag gcggcggcgg gtcagcgcct tggccgtctc gaccctcgcc
     1261 tcgtcgaagc ggccctcggc ctcgagctcc tgggcgcgct gctcgcacac tgccgggtcc
     1321 tcccggtcga gctcgtcggc cagcagggcg tgctcggcga ggacgtcgac cctggtcacg
     1381 ccctcctcag ggctggcgat gtggaggaag ccgccgtccg cctcgatgaa gaccggcccg
     1441 accgcgagcg cggcgagcag cggcgcgtgg ccgggcagga cgccgaggtc gccgtcgacc
     1501 ccgcgggcga tcacgaagct cgcctccccc gtccacacct cccgctcggg ggtgaccagg
     1561 tggaccacca ggttcgccac ggctagccct cgccctcgat ctgcttcgcc ttctcctcgg
     1621 cctcctcgat gccgccgacg ttgaagaacg cctgctcggg aaggtggtcg tactcgccgt
     1681 cggcgagcgc ctggaacgag gcgatggtct cctcgagcgg gaccgtcttg cccttcaccc
     1741 cggtgaaccg ctcggcgacg tagaagggct gggagaggaa gcgctggatc cggcgggcgc
     1801 ggttgaccgt gacccggtcc tcctcgctga gctcgtcgac cccgaggatc gcgatgatgt
     1861 cctgcaggtc gttgttgcgc tggaggatct gctggacccg ccgggcgacg ccgtagtgct
     1921 cgtcgccgac gtagcggggg tcgaggatgc gcgaggtcga ggcgagcggg tcgacggccg
     1981 ggtagatgcc gagctgcgag atctcgcggc tgagcaccgt ggtcgcgtcc aggtgcgcga
     2041 acgtcgtgtg gggggccggg tcggtgatgt cgtcggccgg cacgtagatc gcctgcagcg
     2101 aggtgatcga gtggcccccg gccgaggtga tccgctcctg cagctcgccc atctcgtcgg
     2161 ccagggtcgg ctggtagccg accgcgctcg gcatccggcc gagcagggtg gagacctcgg
     2221 agccggcctg gacgaagcgg aagatgttgt cgatgaacag caggacgtcc tggcccatct
     2281 cgtcccggaa gtactccgcc atcgtgagcg cggccaggcc gacccggagg cggacgccgg
     2341 gcggctcgtc catctggccg aacatcagcg cggtcttctc gatgaccccc gactcggtca
     2401 tctcccggaa caggtcgttg ccctcgcggg tgcgctcccc caccccggcg aacaccgaca
     2461 cgccgctgtg ctcctcggcg acccggtgga tcatctcctg gatgaccacg gtcttgccga
     2521 ccccggcgcc gccgaacatg ccgatcttgc cgccctgcac gtagggctcg agcaggtcga
     2581 tgaccttgat gccggtctgg aacatctcgg tcttggactc gagcttgtcg tacggggggg
     2641 agtggcggtg gatgggccag cgcacgtcgg cctcgaagtc ggggtcgtcg agggcctggc
     2701 ccagcacgtt gaacacgtgc ccggtggtct tctccccgac cggcacggtc atcgactcgc
     2761 cggtgtcggt caccggcgcg ccccggacga ggccgtcggt cggctgcatg gcgatggcgc
     2821 ggacggtgtt gtcgccgagg tgctgcgcga cctcgcaggt gatgacggtg gtctgctcgc
     2881 cgatcgtccg ctcgacggtg acggcgttga agatcgccgg gatctcgtcc ggcgcgaact
     2941 cgacgtcgat caccgggccg atgacccgga cgatccgccc cgtcccctgc tgcgtgtcct
     3001 gcccgacggc tacggtggcc atgttcgtct cagctcccgg tcttctggag ggcttccgcg
     3061 cctccgacga tctccatgat ctcctgggtg atctccgcct gcctcgcctg gttgtactcc
     3121 cgggtgagct tctcgatcag cttctcggcg ttgtcggtgg cggcgctcat cgccttctgc
     3181 ctggaggcgt gctcggaggc ggccgcctcg agcatcgcgg tgaacacgcg cgcctcgacg
     3241 tagcggggca gcagggcgtc cagcaccgcc tgggcgtccg gctcgtagat gtacatcggg
     3301 atgggctgct ccggccgctc gcgggtctcc tcgacgacga gcgggatgaa ccgggtggcg
     3361 gtcgagcgct gcgtcatggc cgagacgtag tcggtgaaca cggcatggat ctcgtccacc
     3421 tcatggtcga ggaacgcctc gagcatcgtg tcggcgaccc gcttggcgtc ctcgtaggag
     3481 ggccggtcgg agaagcccgt ccacgactcg gcgaccgggc gctgccggaa ccggtagtag
     3541 gcgaggccct tgcggccgac cacgtagagc ctcggctcgg tctcctcctg gcgcagccgg
     3601 gccaggagct cctcggtggt gcgcaggacg ttggcgttgt agggaccggc gaggccccgg
     3661 tcggaggtga ccacgagcac ggccgcggcc gacacctcgg cccgctgctc gaggagcggg
     3721 tgggagatgg tgatctcggt gttggcgacg ctcgtgagcg cccgggtcac ctcttcggtg
     3781 tacgggcgcg cctgctgtac ccgctgctgg gcgcgcgcca cccgggaggc ggagatgagc
     3841 tgctgggccc gggtgatctt catggtcgac tgcaccgagc ggatgcgccg gcggacgacc
     3901 ctgagctggg ctgccatggt ctacggggcc ggggtggggc cgcggcggcc ggcggggccg
     3961 gcctgctggt cgatctcctc ggggctgcgg cggtagcgct tcaccgagtc ccgctcctcc
     4021 tcgccctcct ccatggcgtg ggcctcgcgc tccttcggcg gccgctcgcc gtcggaggtc
     4081 ctgaacctgc ccttgaactc ctcgacggcg tccttgagct tggcctcgac gtcctcgggg
     4141 agggtgccct cgttgcggat gtgctcgccg atctcgggat gctcggcgtc catggactcg
     4201 agcagctcgg actcgaaccg gcgcacgtcg gagaccggga tcgagtcgag gtagcccttg
     4261 gtgccggccc agagcgagat gacctgcttc tcgaccggca tcgggtcgta ctggccctgc
     4321 ttcagcagct cgaccatgcg ggcgccccgc tcgagctgcg ccttggacgc ccggtcgagg
     4381 tcggaggcga acagcgcgaa cgcctccagc gcgcggtact gggccaggtc gatgcggagg
     4441 gtccccgcca cctgccgcat cgccttgatc tgggcgctgc ccccgacccg ggagaccgag
     4501 gtgccgacgt tgatcgcggg ccgcacgccc gagttgaaca ggtcggactc caggtagatc
     4561 tggccgtcgg tgatcgagat cacgttggtc gggatgtagg cggagatgtc gttgcccttg
     4621 gtctcgatga tcggcaggcc ggtgagcgag ccgccgccca ggtcgtcgga gagcttggcg
     4681 cagcgctcca gcagccggga gtgcaggtag aacacgtcgc ccgggtacgc ctcgcggccg
     4741 gcgggccggc gcagcaggag cgagatctcg cggtaggcct cggcctgctt ggagaggtcg
     4801 tcgaagacga tcatggcgtg ctcgccctgg tacatccagt gctggccgag ggcggagccc
     4861 gagtaggggg cgatgtactt gaacgaggcg ggcagcgagg cgggcgcgtt caccaccacc
     4921 gtgtactcga gcgcgccgtt ctcctcgagc gccccgacca gctcgcggac ggtggacgac
     4981 ttctggccga ccgcgacgta gatgcacttg acctgcctgt cggtgcccca gtgctggcgc
     5041 tggttgatga tcgcgtccag gcacacggcg gtcttgccgg tcttctggtc gccgatgatc
     5101 agctcgcgct ggccgcgccc gatgttggtc atcgcgtcga tcgccttgat gccggtgtcg
     5161 agcggctccc tgacctcctg gcgctcgacg acgctgggcg cctgcacctc gagggggcgg
     5221 gtctcctcgg tctcgatctc gcccttgccg tcgatcgggc ggccgagcgg gtcgatcacg
     5281 cgcccgagga aggcgtcgcc gaccgggacg gacaggatgc ggcgggtgtg gcggacctcg
     5341 tcgccctcct cgatctcgga ggcgtcgccg aagacgatgc agccgacctc gcggacgtcc
     5401 aggttgaggg ccatcccgag caggccgccg cggaactcga gcagctcgct ggccatggtc
     5461 gtcggcatgc cctcgacgta ggcgatgccg tcgccggtct gggtgacccg cccgacctcc
     5521 tcgcgggcga ccgagggccg gaacgattcg acgtgcttgc gcagggccgc ggcgatgtcg
     5581 tcgggccgga tggacagctc ggtcaccgtt gcacttcctc tcgtgaggct cttctctcgg
     5641 cgggggccgg gctccggtcg ggtcggcggc tggggtcggg cgggcagggt caggcgacgc
     5701 cgagcgcctc gcgggccagc tcgagcctgg tgcggacgct gccgtcgagc agctcgtcgc
     5761 cgatgcggac gaccacggaa cccaaaatag ccggatcgac ggtgcagcgc agctcgaccg
     5821 gcttgccgac catgcgggtg aggacctcgg cgaggcgggc gcggcgctcg ccgtcg
//
LOCUS       DASPYY010000802         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1119137, whole genome shotgun sequence.
ACCESSION   DASPYY010000802 DASPYY010000000
VERSION     DASPYY010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119137"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VEP73_12535"
     CDS             complement(<1..653)
                     /locus_tag="VEP73_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT27298.1"
                     /translation="MTPSADAGATLPVPEDLPPALSSMWRLCKLGYRHEPRLLVAAFV
                     LSLAAALPDALLALWLKLLGDGVLDRDRRLLVVAAVGLGVSATATWILRTVSTRVQRR
                     FRDRVTIALESHVARLQASVATIAHQERPDYLDRLAVLRDQVFVLDHMYMSLFSTAGW
                     ILRLAVTVGLLVSIHPALAVLAVFALPTVLTSTWRPAVERGAEERGAQSNRLARHLFV
                     "
     gene            complement(637..2463)
                     /locus_tag="VEP73_12540"
     CDS             complement(637..2463)
                     /locus_tag="VEP73_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27299.1"
                     /translation="MSRWGGGAIRRLRARPEWKFFGVLPRADGAQAAAWWVVLVLRGV
                     LPAVFAIAMGALVGAVQRGDDLAGPLVAVGAVFVLLQVLAPIHQAVGANLGDRTAAWL
                     YDRLAEACVGPPGMGHLEDPSLAGDLTAARDFDLGTTGPPLSVSMDFIASGLVEMLGG
                     AASAVVLAAYAWWAPILLAGAWLSTHWLLRESSVWRDRNTEEVRSAQRDSDYAYRLAV
                     DPPAAKELRLFGLARWTIERFTARRTRLHELQYQATKLREKPLLWSLLLVLAANLVVF
                     WSLADAAAGGRLGLGRVVMFAQVAVGTSMIAFGGLNWALDGASAPVAASLRLAPAMGA
                     AGALPSGTREADGMPRVGIRFRNVDFAYTGLGAPAVLEGFDLAIPAGSSLAIVGQNGA
                     GKTTLAKLLCRLYDPQRGAIEVDGVDLRDLDLDRWRSRVTAVFQDFTRFELPLRDNVA
                     PAGADDGAVLAALASAGASGLADLDTVLARGYEGGTELSGGQWQRVALARALCAVRLG
                     AGVVLLDEPTAQLDVRGEAEIFDRILTATRRCTTILISHRFSTVRHADRICVLEHGRV
                     LELGTHAELMALGGRYRTMFDLQAQRFGATEDEEGAAYDTLG"
     gene            complement(2484..>3834)
                     /locus_tag="VEP73_12545"
     CDS             complement(2484..>3834)
                     /locus_tag="VEP73_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HYT27300.1"
                     /translation="PTGKGNGHLWPVLAGERAEHHVALGDRRTAAQLADAMRRFASGF
                     GLVPEQGWENPDLPASPFGTPPEVASIGFANGKPAGSASPLTWSAAQYVRLVRDLAAG
                     LLLEQPANTVRRYVSNRQQATTLTVTQPTDKSSVPGSPVTVRGTSAPGNTIDVSATNT
                     DANSANTVVTTTAAADGSFSVDVPIAGGTIVLDVVATSPQGATAHAQRTVVFDFTPGT
                     VLLDTADPTGDDNGPGNYAYPTSTFFQPGAYDIQRFQVLDDGTDIIFRLQTRNLSPTF
                     GSPLGAQLVDVYVHDPGAASTSMLASFPQRNYLIAAGSAWNRLIEVQGFGQRYVDAAG
                     ATLGTVAIRANEISRFITFSVPKLSLGQPGPGWGFTVVLTGQDGFSPDQARGFAATPQ
                     EFLFGVCATASSDPHCTVDPATVPKALDVLTPPGVGQAQELDYTINQPVTLQGVFIP"
BASE COUNT          544 a         1336 c         1494 g          460 t
ORIGIN      
        1 acgaacaggt ggcgggccag ccggttggac tgcgcgccgc gctcctcggc gccgcgctcg
       61 accgcgggcc gccaggtcga ggtcaggacc gtcggcaggg cgaacaccgc cagcacggcg
      121 agcgccgggt ggatcgacac cagcagcccg accgtcacgg ccagccgcag gatccacccc
      181 gcggtggaga agagcgacat gtacatgtgg tcgagcacga acacctggtc gcggagcacg
      241 gccagacggt cgaggtagtc gggccgctcc tggtgggcga tggtcgccac cgacgcctgc
      301 aggcgggcga cgtgggactc gagggcgatc gtcaccctgt cgcggaagcg gcgctggacc
      361 cgggtgctga ccgtgcgcag gatccaggtg gcggtcgccg acacaccgag gccgaccgcg
      421 gcgacgacga gcaggcgccg gtcgcggtcg agcaccccgt cgccgaggag cttgagccac
      481 agcgccagga gcgcgtccgg gagcgcggcg gccagggaca gcacgaaggc ggcgacgagc
      541 agccgcggct cgtggcggta gccgagcttg cacagccgcc acatcgacga cagcgccggg
      601 ggcaggtcct cggggacggg cagggtggcg ccggcgtcag ccgagggtgt cataggcggc
      661 gccctcctcg tcttcggtgg cgccgaaccg ctgggcctgg aggtcgaaca tcgtgcggta
      721 gcggcctccg agcgccatca gctcggcgtg ggtgccgagc tcgaggacgc ggccgtgctc
      781 gagcacgcag atgcggtcgg cgtggcgcac cgtggagaac cggtgcgaga tgaggatcgt
      841 ggtgcaccgg cgcgttgcgg tgaggatgcg gtcgaagatc tcggcctccc cccgcacgtc
      901 gagctgggcg gtcggctcgt cgagcaggac cacgccggcg ccgagccgca ccgcgcacaa
      961 cgcccgggcc agcgcgaccc gctgccactg gccccccgag agctcggtgc cgccctcgta
     1021 gccgcgcgcc aggaccgtgt cgaggtccgc caggccgctg gcaccggccg aggccagcgc
     1081 ggcgaggacg gcgccgtcgt ccgcgccggc gggagccacg ttgtcgcgca gcggcagctc
     1141 gaagcgggtg aagtcctgga acacggcggt gacccgcgag cgccagcggt cgaggtcgag
     1201 gtcgcgcagg tcgacgccgt cgacctcgat ggcgccgcgt tgcgggtcgt agagccggca
     1261 cagcagcttg gccagggtgg tcttgcccgc gccgttctgg ccgacgatgg ccagcgacga
     1321 gccggccggg atggccaggt cgaagccctc gagcaccgcg ggcgcgccca agcccgtgta
     1381 ggcgaagtcg acgttccgga agcggatccc gacccgcggc atgccgtcgg cctccctggt
     1441 gccggacggg agcgcgccgg cggcacccat ggccggcgcg aggcgcagcg acgcggccac
     1501 cggggcggac gcgccgtcga gggcccagtt gagcccgccg aaggcgatca tgctcgttcc
     1561 cacggcgacc tgcgcgaaca tcacgacccg gcccagcccg agacggccgc ccgcggcggc
     1621 gtcggcgagc gaccagaaca ccaccaggtt ggcggcgagg accagcagca ggctccagag
     1681 cagcggcttc tcccgcagct tcgtggcctg gtactgcagc tcgtgcagcc gcgtgcgacg
     1741 ggccgtgaag cgctcgatcg tccagcgggc gaggccgaag aggcgcagct ccttggccgc
     1801 gggcggatcg acggcgagcc ggtaggcgta gtcggagtca cgctgggcgg accgcacctc
     1861 ctcggtgttg cggtcgcgcc acaccgagct ctcgcgcagc agccagtggg tggacagcca
     1921 tgcgccggcc agcaggatcg gcgcccacca cgcgtaggcg gcgagcacca cggcggaggc
     1981 ggcgccaccg agcatctcga ccaggccgct ggcgatgaag tccatcgaga ccgacagcgg
     2041 cgggccggtc gtgcccaggt cgaagtcgcg ggccgcggtg aggtcgcccg cgagcgaggg
     2101 atcctcgagg tggcccatgc ccggcggtcc gacgcacgcc tcggccaacc ggtcgtagag
     2161 ccaggcggcg gtgcggtcgc cgaggttggc gccgaccgcc tgatggatag gggcgagcac
     2221 ctgcagcagc acgaacacgg cgccgaccgc gacgagcggc ccggcgaggt cgtcgccgcg
     2281 ctggacggcg ccgaccagcg cgcccatggc gatggcgaac accgcgggga gcaccccgcg
     2341 cagcaccagc accacccacc aggccgcggc ctgggcgccg tcggccctgg ggaggacgcc
     2401 gaagaacttc cactccgggc gcgcgcgcag acgccggatc gccccgcccc cccagcgcga
     2461 cacacccgcc ccggtccgcc cggctatggg atgaacaccc cctgcagggt caccggctgg
     2521 ttgatcgtgt agtcgagctc ctgggcctgg cccacgccgg gcggcgtgag cacgtcgagc
     2581 gccttcggca cggtggcggg gtcgacggtg cagtgcgggt cgctcgaggc ggtggcgcac
     2641 acgccgaaca ggaactcctg cggcgtcgcc gcgaagcccc tggcctggtc ggggctgaac
     2701 ccgtcctgcc cggtcagcac cacggtgaag ccccagcccg gccccggctg gccgaggctc
     2761 agcttcggca cgctgaaggt gatgaaccgc gagatctcgt tcgccctgat cgcgaccgtc
     2821 ccgagcgtgg cgccggccgc gtcgacgtag cgctgcccga acccctgcac ctcgatcagc
     2881 cggttccagg ccgaccccgc cgcgatgagg tagttccgct gcgggaagga cgccagcatg
     2941 gaggtgcttg ccgctcccgg gtcgtggacg tagacgtcca cgagctgcgc cccgagcggg
     3001 ctgccgaagg tcgggctcag gttgcgggtc tgcagccgga agatgatgtc ggtgccgtcg
     3061 tccaggacct ggaagcgctg gatgtcgtag gcgcccggct ggaagaaggt ggaggtcggg
     3121 taggcgtagt tgcccggccc gttgtcgtcc ccggtcgggt cggcggtgtc gagcagcacc
     3181 gtgcctgggg tgaagtcgaa cacgaccgtg cgctgcgcgt gggcggtggc gccctgcggg
     3241 ctggtcgcta ccacgtcgag cacgatcgtc ccgcctgcga tcggcacgtc gacgctgaag
     3301 ctcccgtcgg ccgcggcggt cgtggtcacg accgtgttgg cggagttggc gtcggtgttg
     3361 gtggcggaca cgtcgatggt gttgcccggc gccgaggtgc cgcgcacggt gaccggcgag
     3421 ccgggcaccg agcttttgtc tgtcggctga gtgaccgtga gggtggtggc ctgctgccgg
     3481 ttgctgacgt agcggcgcac ggtgttggcg ggctgctcca gcagcaggcc ggcggcgagg
     3541 tcgcggacca ggcggacgta ctgggcggcc gaccaggtga gcggcgaggc cgagcccgcc
     3601 ggcttgccgt tcgcgaagcc gatcgaagcg acctcgggtg gggtgccgaa cggcgacgcc
     3661 ggcaggtcgg ggttctccca gccctgctcg gggacgaggc cgaagccgga ggcgaagcgc
     3721 cgcatcgcgt ccgccagctg ggcggccgtc ctccggtcgc cgagggcgac gtggtgctcg
     3781 gcgcgctcgc cggccaggac cggccagaga tgcccgttgc ccttgccggt cggc
//
LOCUS       DASPYY010000803         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1542451, whole genome shotgun sequence.
ACCESSION   DASPYY010000803 DASPYY010000000
VERSION     DASPYY010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542451"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VEP73_12550"
     CDS             complement(<1..495)
                     /locus_tag="VEP73_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT27301.1"
                     /translation="MTVDETSGRLTHRQILIVFSGLMSGMLLAALDQTIVATALPTIV
                     GDLGGLSHLSWVVTAYLLASTASTPLYGKISDLYGRKSVFQAAIVIFLVGSALSGMAH
                     TMAQLIAFRAVQGVGAGGLMALAMAIIGDVVSPRERGRYVGYLGAVFGLASVVGPLLG
                     GFFVD"
     gene            622..887
                     /gene="ffs"
                     /locus_tag="VEP73_12555"
     ncRNA           622..887
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEP73_12555"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1078..3141
                     /gene="dnaX"
                     /locus_tag="VEP73_12560"
     CDS             1078..3141
                     /gene="dnaX"
                     /locus_tag="VEP73_12560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYT27302.1"
                     /translation="MAYVSLYRKWRPQTFSDIVGQDHVVGTLVNAIRTGQVHHALLFT
                     GTRGTGKTSTARILAKALNCERGPTAEPDNTCAQCVAITDGTSLDVIEIDAASHGSVD
                     DARDLREKANYAPASARYKVYIVDEAHMVTTQGFNAMLKVLEEPPGHVRFIFATTEPH
                     KVIEAIRSRCQQHDFRRVRAAVLVDHFAKVCAAQGVDVDQPALEMIARAADGSVRDGM
                     GRLDQVLTYAGGRVRLADVAAVLGALPIELRFELAEALASHRVADVFACVERVSQGGH
                     DLHQFAREALEHVRDLYVLRVAPESSGLVDVTAETRERLAAQAERFGPGELGRLVRIL
                     GDLYLDLRTATDQRLVLEIGLARAALPEASLDADALLARIERLERRLSIAADPAAPGS
                     PPGPPAPASTAPRSAPAGQAAPASPDRATPGGPAAVPPGGAQVPPAQVPPTPPTPPTP
                     PAQGPAAEGGGRKAGRAGRAAASKARQAPPDPAVDGDWAPAREAPSVPVDLDLVLRSW
                     SLVVDRVRAASRVAATYLENGRPVAVEGRQVVVEFPPESRFYADALSRDGRSKRVDAA
                     LENVLGGDLEVRIRLGEAAGALAPAVAPDVDEPAPAPPGEPAPAVHATEFEVDPANDE
                     GEQLDSDADARAVADWAARELAGEVIEERPNEAAGRGAGESRTKERAAGGRRSSGPPR
                     ERGRR"
     gene            3168..>3394
                     /locus_tag="VEP73_12565"
     CDS             3168..>3394
                     /locus_tag="VEP73_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYT27303.1"
                     /translation="MGRGGFNPNQMLKQLQKVQQQMEDTQAQLAEEVVEGTAGGGVVI
                     ARVKGDNTVESVTIDPAALDPDDVEMLGDLVT"
BASE COUNT          473 a         1285 c         1235 g          401 t
ORIGIN      
        1 gtcgacgaag aagccgccga gcagcggccc gaccacgctg gcgagcccga acacggcacc
       61 gaggtagccg acgtaccggc cgcgctcccg tggcgagacg acgtcgccga tgatcgccat
      121 ggcgagcgcc atgaggccgc cggcaccaac gccctggacg gcccggaaag cgatcagctg
      181 ggccatcgtg tgggccatcc cggacagggc cgagccgacc aggaagatga cgatcgcggc
      241 ctggaacacg ctcttgcggc cgtacaggtc gctgatcttg ccgtacagcg gggtcgaggc
      301 ggtcgaggcg agcaggtagg cggtcaccac ccaggacagg tgggagaggc cgccgaggtc
      361 gccgacgatg gtcggcagtg cggtggccac gatcgtctgg tccagggctg ccagcagcat
      421 gccggacatg agcccgctga agacgatcag gatctgcctg tgggtgagcc gcccgctcgt
      481 ctcgtcgacc gtcatgcgcc cctccgtgcc gtgggtcctt gcccccaacg tagagcagtc
      541 tgcttgtagc atgcaaccag tttcccggta gatgcgcggg ctggcgagga cggggcgcga
      601 gcgcgtaggc tccagctcgg accgtgctag gtggggagct agcggtgccc tgcaacccgc
      661 aatccgctcc agcggggctg aatccccacc cgagggctcg cgtccgaagg tcagcgccgg
      721 gtacggagtg ttgacggtca ggtcccgcgc aacgtcacct tgcgaaccgc gtcaggtccg
      781 ggaggaagca gcgctaagca ggcactggcg ggtgccgcgg ggttgccggg cccgagccac
      841 cggctcggta ggcgcttcga gcgtcgggtt cgaaggtggg tgcacggtcc acccacgtcg
      901 gcgagggccc gggggcagct cccccgggcc ctcgccgtgc cgaaggcgga agttccgcag
      961 tgtgcgccgg gtgccggccg gctcgggatc gtcgggcacg gatggtatct tgggcgccgc
     1021 cggagccctc cggctcccat ctcctccctg accggcgccc tgcgcccttc ctgagctatg
     1081 gcgtacgtct ccctgtaccg aaagtggcgg ccgcagacct tctccgacat cgtcgggcag
     1141 gaccacgtgg tcgggacgct ggtgaacgcg atccgcaccg gccaggtgca ccacgccctg
     1201 ctgttcaccg gcacccgggg aacgggcaag acgtcgaccg cccgcatcct cgccaaggcg
     1261 ctgaactgcg agcgcggccc cacggcggag ccggacaaca cctgcgcgca gtgcgtcgcc
     1321 atcaccgacg gcaccagcct cgacgtgatc gagatcgacg cggccagcca cggctcggtc
     1381 gacgacgccc gcgacctgcg cgagaaggcg aactacgcgc cggcctcggc ccgctacaag
     1441 gtctacatcg tcgacgaggc ccacatggtc accacccagg gcttcaacgc gatgctcaag
     1501 gtgctcgagg agccgcccgg gcacgtccgc ttcatcttcg ccaccaccga gccgcacaag
     1561 gtcatcgagg ccatccggtc gcgctgccag cagcacgact tccggcgggt gcgggccgcc
     1621 gtgctcgtgg accacttcgc caaggtgtgc gcggcccagg gggtggacgt cgaccagccc
     1681 gcgctcgaga tgatcgcccg cgcggccgac ggctccgtgc gcgacggcat gggccgcctc
     1741 gaccaggtcc tcacctatgc cggcgggcgg gtgcggctgg ccgacgtcgc cgccgtcctc
     1801 ggcgcgctgc cgatcgagct gcgcttcgag ctggccgagg cgctcgcgtc ccaccgggtg
     1861 gccgacgtct tcgcctgcgt cgagcgggtc tcgcagggcg gccacgacct ccaccagttc
     1921 gcccgggagg cgctcgagca cgtgcgcgac ctgtacgtgc tgcgggtcgc gcccgagtcg
     1981 tccggcctgg tcgacgtcac cgccgagacc cgcgagcggc tcgccgccca ggccgagcgg
     2041 ttcggccccg gcgagctcgg ccgcctcgtc cgcatcctgg gcgacctcta cctcgacctg
     2101 cggacagcca ccgaccagcg gctcgtcctc gagatcggcc tcgccagggc cgccctcccc
     2161 gaggcctccc tggacgccga cgccctcctc gcccgcatcg agcggctcga gcggcgcctg
     2221 tcgatcgccg ccgacccggc cgcccccggc agcccgcccg gcccgccggc cccggccagc
     2281 acggcgcccc gctccgcgcc ggccggccag gcggcccccg cctccccgga ccgggccacc
     2341 ccgggcggcc cggccgcggt ccctcccggc ggcgcccagg tgccgccggc ccaggtgccg
     2401 ccgaccccgc cgaccccgcc gaccccgccg gcccagggcc cggcggccga gggcggcggg
     2461 cggaaggccg gacgtgcggg ccgggccgcc gcgtccaagg cccgccaggc gccgcccgac
     2521 ccggccgtcg acggcgactg ggcgcccgcg cgggaggcgc cctcggtccc cgtcgacctc
     2581 gacctcgtcc tgcggtcatg gtcgctggtc gtcgaccggg ttcgggcggc cagccgggtc
     2641 gccgcgacct acctggagaa cggccgcccc gtcgccgtgg agggccgcca ggtggtggtc
     2701 gagttcccgc ccgagagccg cttctacgcc gacgcgctct cccgcgacgg ccgcagcaag
     2761 cgggtcgacg ccgcgctcga gaacgtgctc ggcggcgacc tcgaggtccg gatcaggctc
     2821 ggcgaggccg ctggcgccct ggcgccggcc gtcgcccccg acgtcgacga gcccgcgcca
     2881 gccccacccg gcgagccggc acccgcggtc cacgcgaccg agttcgaggt cgacccggcc
     2941 aacgacgagg gcgagcagct cgactcggac gccgacgccc gggcggtggc agactgggcc
     3001 gcccgggagc tcgccggcga ggtcatcgag gagcggccca acgaggccgc cggccgcggc
     3061 gccggggagt ccaggacgaa ggagcgggcg gcgggaggcc gcaggtccag cggcccgccc
     3121 agggaaaggg gaaggcgttg agcatggctc gcggtggcgg tggtggcatg ggtcggggtg
     3181 gcttcaaccc caaccagatg ctcaagcagc tgcagaaggt ccagcagcag atggaggaca
     3241 cccaggccca gctggccgag gaggtcgtcg agggcacggc cggcggcggc gtggtgatcg
     3301 ccagggtcaa gggtgacaat accgtcgagt cggtcaccat cgacccggcc gccctcgacc
     3361 ccgacgacgt cgagatgctc ggggacctcg tgac
//
LOCUS       DASPYY010000804         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2524208, whole genome shotgun sequence.
ACCESSION   DASPYY010000804 DASPYY010000000
VERSION     DASPYY010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524208"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..593)
                     /locus_tag="VEP73_12570"
     CDS             complement(144..593)
                     /locus_tag="VEP73_12570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYT27304.1"
                     /translation="MRTARAVSAGGVVLAEPRADARVALVARRSARGALQWTLPKGQQ
                     EPGETVAETALREAREETGLQVELLNPLDTIDYWFVWAPEQTRYHKFVHYFLMRAVGG
                     DFALHDAEMEDAGWFTPQEARRVMAFANECRLLDLIAGALADAGVAG"
     gene            complement(615..1100)
                     /locus_tag="VEP73_12575"
     CDS             complement(615..1100)
                     /locus_tag="VEP73_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYT27305.1"
                     /translation="MRSSIDVHNHLLTDDVLHELSALPGTLRDLAAAPAVLGLPPAAV
                     GQPTVFADAEGAVVVLAPAGTEVDLAGVAELLGREQLAPVAPDEAPGLTGYLLPFVPP
                     VALECDSRLVVDERLAGQDVVYTAAGEPGVILKVRGADLVKSTSAAVFGVTRPVDDRR
                     G"
     gene            complement(1278..1469)
                     /locus_tag="VEP73_12580"
     CDS             complement(1278..1469)
                     /locus_tag="VEP73_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27306.1"
                     /translation="MADAKKMSRAEAGRKGGQTTKKRYGDDFFGKIGRMGGKKGGETT
                     KQRYGIEFYQKIGRKGGSR"
     gene            1677..2750
                     /locus_tag="VEP73_12585"
     CDS             1677..2750
                     /locus_tag="VEP73_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYT27307.1"
                     /translation="MDVGELLGHLVERQGSDLHLKAGSVPYVRVDGELVGTSFPLLTE
                     ADTERIAVGLMDGRQAARFTESNEADFACTLPGLGRFRVNAFRQRGAVGLVCRRVLPR
                     SPGFEALGLPSVVARMAEEPRGLILVTGPTGSGKSTTTAAVIDHINAVRRCHVVTIED
                     PIEVLHTDNLAVIDQREVGVDTADFATALKYVARQDPDVIFIGEMRDEETTKAALQAA
                     ETGHLVISTLHTADAAETVNRVLEFFPPHQQRQIRLALAGTLRCVVSQRLLPRVDGRG
                     RVAAVEIMVATGRVFDFIVNPEHTHLLPEVVAEGEFYGMQTFDQALLALYRDGLIGLD
                     AATLAASNPHDFRISVQQAGLPL"
     gene            2841..>3078
                     /locus_tag="VEP73_12590"
     CDS             2841..>3078
                     /locus_tag="VEP73_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HYT27308.1"
                     /translation="MTTLSPAQREALATLLRASPFAGVARELGERFAEAGHELYLVGG
                     TVRDALLDRSPPDVDLTTDASPDQVLAVVRAGGWD"
BASE COUNT          394 a         1159 c         1095 g          430 t
ORIGIN      
        1 gacccagggc gagatctgca ggaggccggc ggccatgacc cggccctggc cctgctcctg
       61 ggcgtgggcg ggcgaggccg ccagcccacc cgcggtggcg gtcagggcca cggccaccgc
      121 cagcgcgccc aggagccgcc ggatcacccg gccacgcccg cgtcggccag ggcgccggcg
      181 atcaggtcga gcagccggca ctcgttggca aaggccatca cgcggcgggc ctcctgggga
      241 gtgaaccagc cggcatcctc catctcggcg tcgtgcagcg cgaagtcgcc accgacggcg
      301 cgcatgagga agtagtggac gaacttgtgg tatcgggtct gctccggcgc ccacacgaac
      361 cagtagtcga tggtgtcgag cgggttgagc agctcgacct ggagcccggt ctcctcccgc
      421 gcctcgcgca gcgcggtctc ggccaccgtc tcgccgggct cctgctggcc cttgggcagc
      481 gtccactgca gcgccccacg ggccgaccgg cgggcgacga gggcgacccg ggcgtccgcc
      541 cgcggctcgg cgagcaccac cccgccggct gagaccgcgc gggcggttcg cacggctagc
      601 ccctccggac ggtgctagcc gcggcggtca tcgaccggcc tggtcacgcc gaagaccgcg
      661 gcgctggtgg acttgaccag gtccgcgccc cgcaccttga ggattacgcc tggctcgccc
      721 gcggccgtgt agacgacgtc ctgcccggcc aggcgttcgt cgaccaccag cctgctgtcg
      781 cactcgagag ccaccggggg gacgaacggg agcaggtagc cggtcaggcc cggagcctcg
      841 tccggagcca ccggggcgag ctgctcgcgg ccgagcagct cggccacgcc ggccaggtcg
      901 acctcggtcc cggccggggc gaggaccacg actgcgccct cggcgtcggc gaacacggtc
      961 ggctgcccca cggcagcggg cgggaggccc aggacggcgg gtgcggcggc caggtcgcgc
     1021 agcgtccccg gaagggcgga gagctcgtgg agcacgtcgt cggtgagcag gtggttgtgc
     1081 acgtcgatgc tggagcgcac tcgttctcca tggagggtgt gaagcaacgg cccgggcata
     1141 cgagtatacc ggggcggcct gcacaggcgg cctgccgggg cggcctgtcc taccctaccc
     1201 gggccgccgg gcagccgccg gccgggacgc gcgtcccggc cggcggctgc gtgcaggtgc
     1261 gccggaaggg ggctcgctca gcgagaaccg cccttcctgc cgatcttctg gtagaactcg
     1321 atcccgtagc gctgcttggt ggtctcgcca cccttcttgc cgcccatgcg cccaatcttg
     1381 ccgaagaagt cgtcgccgta gcgcttcttc gtggtctggc cgcccttccg tcctgcctcg
     1441 gcgcggctca tcttcttcgc atccgccatc ccgtccacct cctgctcttc ggttgccctg
     1501 agtggttttg cctaaaccac ttgaattggt tgggcttcgg gcggcgggcg tcaacccggc
     1561 ctgtctgtcg cccgatgagg acggggaggc gaggagagga cagggcacgc ccgggggctc
     1621 gcggcctccg ggagacggga ggtggcgtgg acgtttctcc ggtggacgct cctccggtgg
     1681 acgtcgggga actgctcggc cacctggtcg agcgccaggg gtccgacctg cacctgaagg
     1741 ccggctccgt gccctacgtg cgggtcgacg gcgagctcgt cggcacctcg ttccccctgc
     1801 tcaccgaggc cgacaccgag cggatcgcgg tcggcctcat ggacgggcgc caggccgcca
     1861 ggttcaccga gtccaacgag gcggacttcg cctgcacgct gcccggcctc ggccgcttcc
     1921 gcgtcaacgc cttccggcag cggggcgccg tcgggctggt ctgccggcgc gtgctgcccc
     1981 gcagcccggg gttcgaggcg ctcggcctgc cttccgtcgt cgctcgcatg gcggaggagc
     2041 cgcgcggcct gatcctcgtg accggcccca ccgggtcggg caagtccacc accacggcgg
     2101 cggtcatcga ccacatcaac gcggtccggc gctgtcacgt cgtgaccatc gaggacccta
     2161 tcgaggtcct gcacaccgac aacctggcgg tgatcgacca gcgcgaggtc ggggtcgaca
     2221 ccgccgactt cgccaccgcc ctgaagtacg tcgcccggca ggatcccgac gtgatcttca
     2281 tcggcgagat gcgcgacgag gagacgacca aggccgcgct ccaggcggcc gagaccggcc
     2341 acctggtgat ctcgaccctc cacacggccg acgccgccga gaccgtcaac cgggtcctcg
     2401 agttcttccc gccccaccag cagcgccaga tccggctcgc cctggccggg acgctgcgct
     2461 gcgtcgtctc ccagcgcctg ctgccccggg tcgacggccg gggcagggtg gcggcggtcg
     2521 agatcatggt ggccacgggt cgggtgttcg acttcatcgt caaccccgag cacacccacc
     2581 tgctgcccga ggtggtggcc gagggcgagt tctacggcat gcagaccttc gaccaggccc
     2641 tgctcgcgct ctaccgggac gggctgatcg gcctggacgc cgcgacgctg gccgcgtcca
     2701 acccccacga cttccgcatc tccgtgcagc aggccgggct gcccctctga cggcggcgcc
     2761 ctcggcccga gggcccggcg gcgctctggc ccgacgggag gccgcccagg ccgcttggcc
     2821 gggccggtat cctgcccgcc atgaccaccc tgtcccctgc acagcgagag gccctcgcca
     2881 ccctgctccg ggcatcgccg ttcgccggcg tggccaggga gctcggcgag cgcttcgccg
     2941 aggccggcca cgagctctac ctggtgggcg ggacggtgcg ggacgccctg ctcgaccgct
     3001 cgccgcccga cgtcgacctc accaccgacg cctcccccga ccaggtcctc gccgtggtcc
     3061 gcgcgggggg ctgggacg
//
LOCUS       DASPYY010000805         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1681331, whole genome shotgun sequence.
ACCESSION   DASPYY010000805 DASPYY010000000
VERSION     DASPYY010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681331"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..638)
                     /locus_tag="VEP73_12595"
     CDS             complement(114..638)
                     /locus_tag="VEP73_12595"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYT27309.1"
                     /translation="MTVEEVFPAAPLDPGARLLTAVAGVVAIGGAVALALARPGGLTI
                     LVGAPSLFVLLVALPLAFAPTGYAVGRGELAVLRRGAAPLLFPLGGLAAARPTAMPRS
                     VRLLGSGGLFGWWGRFANRGWGRFRAYATDRRRGVLLEWPGFKLFVSPENPEALCRAV
                     LARRRGAERRGRRS"
     gene            complement(742..>1725)
                     /locus_tag="VEP73_12600"
     CDS             complement(742..>1725)
                     /locus_tag="VEP73_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYT27310.1"
                     /translation="SYMFITGPDVVKAVTGEDVTFEELGGAMTHAVKSGTAHFAAEDE
                     DECFDLTRSLLGYLPLNNMEDPPRHQPTDDPRREDPTLIDLVPDNPNQPYDIREVIKR
                     VVDDGGFLEIQQAWAESIVIGLARLNGRSIGIVGNQPLHLAGVLDINASIKGARFVRF
                     CDAFNIPIVSFVDVPGFMPGTSQEWGGIIRHGAKLLYAYAEATVPKITVITRKAYGGA
                     YDVMASKPLRADVNYAWPTAEIAVMGPQGAVNIIFRREIERSADPAARRDELVAEYTE
                     RFANPFIAAERGYVDEVIEPQLTRPRLCTALEMCMTKRELRPPRKHGNIPL"
BASE COUNT          237 a          624 c          624 g          240 t
ORIGIN      
        1 aatggcgagc acctcgggct cttcgccctc cggcgtgccc ttcacgaccc ggaaccgcag
       61 gccgttcggg gtccgcagca cctcgatgtc gcccggctgc gcctcgtacg cggtcatgat
      121 cttcgtcccc tcctctcggc cccccggcgc cgtgccagca ccgcgcggca gagcgcctcc
      181 gggttctcgg gcgagacgaa cagcttgaac ccaggccact cgaggaggac gccgcggcgc
      241 cggtcggtgg cgtacgccct gaagcggccc cagccccggt tcgcgaaccg cccccaccac
      301 ccgaacagcc cgccggagcc gagcagccgg acgctccggg gcatggcggt cggcctggcc
      361 gccgccaggc cgccgagcgg gaagaggagc ggcgcggcac cgcggcgcag caccgccagc
      421 tcgccccggc cgaccgcgta gccggtcggg gcgaaggcca ggggcagcgc gaccagcagc
      481 acgaacaggc tcggggcgcc gaccaggatg gtgaggccgc ccgggcgggc gagcgcgagg
      541 gcgaccgcgc cgccgatcgc caccaccccg gccacggcgg tcagcagccg cgcgcccggg
      601 tcgagcgggg cggccgggaa cacctcctcg accgtcatgc gcgcccgctc aggccggcga
      661 tcctgccggc ctgagccaga ggatcgcccg cctccggcgg gctccgagcc cgtcacggtt
      721 ccaagccctt caccatgccc ttcacaatgg gatgttgccg tgcttgcggg gcggccggag
      781 ctcgcgcttg gtcatgcaca tctcgagcgc ggtgcacagc cggggccggg tcagctgggg
      841 ctcgatgacc tcgtcgacgt agccgcgctc ggccgcgatg aaggggttcg cgaaccgctc
      901 ggtgtactcg gcgaccagct cgtcccggcg ggcggccggg tcggcggacc gctcgatctc
      961 ccggcggaag atgatgttga cggccccctg gggccccatc accgcgatct cggcggtggg
     1021 ccaggcgtag ttgacgtcgg cccgcagcgg cttggacgcc atgacgtcgt aggcgccgcc
     1081 gtacgccttg cgggtgatca cggtgatctt gggcacggtc gcctcggcgt aggcgtacag
     1141 cagcttggcg ccgtgccgga tgatgccgcc ccactcctgg ctcgtgcccg gcatgaaccc
     1201 gggcacgtcg acgaagctca cgatcgggat gttgaaggcg tcgcagaagc gcacgaaccg
     1261 ggcgcccttg atcgaggcgt tgatgtcgag caccccggcc aggtgcagcg gctggttgcc
     1321 gacgatcccg atcgaccggc cgttcaggcg ggcgaggccg atcacgatcg actcggccca
     1381 cgcctgctgg atctcgagga accccccgtc gtcgaccacc cgcttgatca cctcgcggat
     1441 gtcgtacggc tggttggggt tgtcggggac caggtcgatc agggtcgggt cctcgcgccg
     1501 tgggtcgtcg gtcggctggt gccgcggcgg gtcctccatg ttgttcaagg ggaggtagcc
     1561 gagcagcgag cgggtgaggt cgaagcactc gtcctcgtcc tcggccgcga agtgggcggt
     1621 gccggacttg accgcatggg tcatggcgcc gcccagctcc tcgaaggtca cgtcctcacc
     1681 ggtcacggcc ttcaccacgt ccgggccggt gatgaacatg tagga
//
LOCUS       DASPYY010000806         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1119374, whole genome shotgun sequence.
ACCESSION   DASPYY010000806 DASPYY010000000
VERSION     DASPYY010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119374"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..669)
                     /locus_tag="VEP73_12605"
     CDS             complement(112..669)
                     /locus_tag="VEP73_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132271.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HYT27311.1"
                     /translation="MTTPLTKTARHARIADILAREQVRSQEELADLLERYAGVHVTQA
                     TLSRDLDELGIVRLRSGGALVYSLPEEPGGPGSHPGGPGSPAGAAASQSGARVAGSES
                     PHDARLARYLGELMTSAEASANLVVLRTPAGAAQFLASVIDHAALPSVLGTVAGDDTV
                     LLIARHPAGGDALAADFLRLASRRR"
     gene            complement(666..>1585)
                     /gene="argF"
                     /locus_tag="VEP73_12610"
     CDS             complement(666..>1585)
                     /gene="argF"
                     /locus_tag="VEP73_12610"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYT27312.1"
                     /translation="GSGGDETLIPPAPPRRPLAGPRTVAVLFDKPSLRTRVSFTVGIA
                     ELGGHPLVIDTQTTHIGRGETIEDVARVLTRQVAAIAWRTAGQERIDAMASAATVPVI
                     NALTDQFHPCQVLADLQTIQAHYGSLASRVLTFLGDGSSNMAHSYLLGGATAGMHVRI
                     GAPEAYHPDPAVLAAAARIAAGTGGSAAVLTDARAACQEADVIATDVWTSMGQDSERV
                     QRLAILGSGDSYQLDAAKLALGRPGCLVLHCLPAHRGEEITAEVIDGPNSVAWEQAEN
                     RLHAQKALIAWLCSSGGDDPPEPPRGEQP"
BASE COUNT          191 a          572 c          600 g          222 t
ORIGIN      
        1 cccccgccgt gttctcgctc tccgtgtccc cggaatgctc cgctgcttcc acagtcctac
       61 tttctaccgt acgaacgccg cagttttgtc gcaccaccga accggagccg gttatcgccg
      121 gcggctggcg aggcgcagga agtcggcggc cagggcgtcg ccgccggccg ggtgccgcgc
      181 gatcagcagc accgtgtcgt ccccggccac ggtgccgagc accgaaggca gcgccgcgtg
      241 gtcgatgacc gaggcgagga actgggcggc cccggccggg gtccgcaaca cgaccaggtt
      301 ggcgctcgcc tcggccgagg tcatcagctc gcccaggtag cgggccagcc gcgcgtcgtg
      361 cggcgactcg gacccggcga ccctggcgcc ggactgcgag gccgcggcgc cggcgggcga
      421 gcccgggccg cccgggtgcg agcccgggcc gccgggctct tccggcaggg agtagaccag
      481 cgcgccaccc gagcgcagcc gcacgatgcc gagctcgtcc aggtcccggg acagggtcgc
      541 ctgcgtcacg tgcaccccgg cgtaccgctc cagcaggtcg gccagttcct cctgggagcg
      601 gacctgctcg cgggccagga tgtcggcgat cctggcgtgc ctggccgtct tggtcagcgg
      661 ggtggtcatg gttgttcccc gcgggggggc tcgggggggt cgtccccccc ggaggagcag
      721 agccaagcta tgagggcctt ctgcgcgtgc aggcggttct ccgcctgctc ccaggccacg
      781 ctgttcggcc cgtcgatcac ctcggcggtg atctcctcgc cgcggtgcgc gggcaggcag
      841 tgcaatacca ggcatcccgg ccggcccagg gccagcttgg ccgcgtccag ctggtaggag
      901 tcgcccgagc cgaggatcgc cagccgctgc acccgctcgc tgtcctggcc catcgacgtc
      961 cacacgtcgg tggcgatgac gtcggcctcc tggcacgccg ccctggcgtc ggtgagcacg
     1021 gccgccgagc cgccggtccc ggcggcgatc cgtgcggcgg cggcgagcac cgccgggtcg
     1081 gggtggtagg cctccggggc gccgatccgg acgtgcatcc cggcggtggc gccgcccagc
     1141 aggtaggagt gcgccatatt gctcgagccg tcgcccagga aggtcagcac gcggctggcc
     1201 agcgagccgt agtgtgcctg gatggtctgc aagtcggcca ggacctggca ggggtggaac
     1261 tggtcggtga gcgcgttgat caccggcacg gtcgcggccg acgccatcgc gtcgatgcgc
     1321 tcctgcccgg cggtccgcca ggcgatcgcc gctacctgcc gggtcagcac ccgggccacg
     1381 tcctcgatgg tctcgccccg gccgatgtgg gtcgtctggg tgtcgatcac cagcgggtga
     1441 ccgccgagct cggcgatgcc gacggtgaag gagacccggg ttcgcagcga cggcttgtcg
     1501 aacagcaccg cgactgtccg cgggccggcg aggggcctgc gggggggcgc gggggggatc
     1561 agggtttcgt cccccccgga gccag
//
LOCUS       DASPYY010000807         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1262965, whole genome shotgun sequence.
ACCESSION   DASPYY010000807 DASPYY010000000
VERSION     DASPYY010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262965"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VEP73_12615"
     CDS             <1..362
                     /locus_tag="VEP73_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYT27313.1"
                     /translation="RDFEALTDNPAWAGELRRVYRGDIERVDLMVGMFAERRPDGFAF
                     SDTAFRIFILMASRRLNSDRFLTRDYTPEVYTPAGLDWVERTTMSDVLLRHHPQLRPA
                     LRSVPNAFMAWSRPAPS"
     gene            390..770
                     /locus_tag="VEP73_12620"
     CDS             390..770
                     /locus_tag="VEP73_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27314.1"
                     /translation="MAGARARDVARYALAGIRLFNGTVALLAPAAMAERLGVKPEAAP
                     AAAYALRLFGIRTVVIGVELLVLEGDDLDRSLRNGVLIHASDAVSAATAGARKQLPAT
                     AATTATVISTVNVLLALAARDPKR"
     gene            814..>1587
                     /locus_tag="VEP73_12625"
     CDS             814..>1587
                     /locus_tag="VEP73_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT27315.1"
                     /translation="MTTSVEHEHVRANGITFHVAASGPRDAPPVLCLHGFPEGWMSWR
                     PVMAALPEARVYVPDLRGYPGTDRPRAGYDVLTLTDDIRGLIETLGLDRPLLVSHDWG
                     GALAWIFAHRHSDLIRELVVVNCPHPRTLVRAVLHFEDFQAFRIPWVPFFMVPRVPEL
                     VGTTALGRRALELSFTLREGTGGTMDVALVDELVARFQRPRDLRGPIDYYREMVRTQL
                     VPGRRARLDAVYDTPITVPTTLVWGEKDGALSAKVAKKSG"
BASE COUNT          209 a          602 c          553 g          223 t
ORIGIN      
        1 ccagggactt cgaggcgctc accgacaacc cggcctgggc cggggagctg cgccgcgtct
       61 accggggcga catcgagcgg gtggacctca tggtcggcat gttcgccgag cgccgcccgg
      121 acgggttcgc cttcagcgac acggcgttcc gcatcttcat cctgatggcc tcccgccggc
      181 tcaacagcga ccgcttcctc acccgcgact acacccccga ggtatacacc ccggccggcc
      241 tcgactgggt cgagcgcacc accatgtccg acgtgctgct gcgtcaccac ccgcagctgc
      301 ggccggccct gcgctcggtc ccgaacgcct tcatggcctg gtcccgtccg gccccgagct
      361 agccacgacc ttcagggagg agagacgcga tggcaggagc acgcgctcgc gacgtcgcca
      421 ggtacgccct cgcgggcatc cggctgttca acggcaccgt cgcgctgctg gcgcccgcgg
      481 ccatggccga gcggctcggc gtgaagccgg aggcggcccc ggccgccgcc tacgccctgc
      541 ggctgttcgg catccgtacc gtcgtcatcg gtgtcgagct gctggtgctc gagggtgacg
      601 acctcgaccg gtcgctgcgc aacggcgtgc tgatccacgc cagcgacgcc gtctcggcgg
      661 cgacggccgg ggccaggaag cagctgccgg cgaccgccgc gacgaccgcc accgtcatct
      721 ccaccgtcaa cgtcctgctg gccctggcgg cccgggaccc gaagcgatga gccgaaaggc
      781 gggggcggcc accgcctgcc ggcccggtga cccatgacga ccagcgtcga gcacgagcac
      841 gtccgggcca acggcatcac cttccacgtg gccgcgtcgg ggccgcgcga cgcgccgccc
      901 gtcctgtgcc tgcacgggtt tcccgagggg tggatgagct ggcggccggt gatggcggcg
      961 ctgcccgagg cccgcgtgta cgtgccggac ctccggggct acccgggcac cgatcgtccc
     1021 agggccggct acgacgtcct cacgctgacc gacgacatcc gtgggctgat cgagaccctc
     1081 ggcctcgacc ggccgctgct cgtctcgcac gactggggcg gcgcgctggc gtggatcttc
     1141 gcccaccggc actcggacct gatccgtgag ctcgtcgtgg tcaactgccc gcacccccgg
     1201 acgctcgtgc gggcggtgct gcacttcgag gacttccagg ccttccgcat cccgtgggtg
     1261 cccttcttca tggtgccgcg ggtccccgag ctcgtcggca cgacggcgct gggcaggagg
     1321 gcgctcgagc tgtcgttcac gctgcgggag gggaccgggg gcaccatgga cgtggcgctc
     1381 gtcgacgagc tggtcgcacg cttccagcgg cctcgggacc tgcgcgggcc catcgactac
     1441 taccgcgaga tggtgcgcac ccagctcgtg ccgggccggc gcgcccgcct cgacgccgtc
     1501 tacgacacgc cgatcaccgt cccgaccacg ctcgtgtggg gcgagaagga cggcgcgctc
     1561 tcggccaagg tggcgaagaa gagcggc
//
LOCUS       DASPYY010000808         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2664004, whole genome shotgun sequence.
ACCESSION   DASPYY010000808 DASPYY010000000
VERSION     DASPYY010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664004"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1570
                     /locus_tag="VEP73_12630"
     CDS             347..1570
                     /locus_tag="VEP73_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27316.1"
                     /translation="MPAVMPRHGSRPAHRSGRPRPGSGPTTRSWLRTVLVPYLAATFV
                     LLAVVFAMAPHRTGAPASGTWDYQREVVGIHGGGAGNAYARAAIRATAWMTKGSIERQ
                     RDLFRVNLLGFLAGRPLAGGVGAWRDAHRLPLEGGTIAVLVGLIAYGVLRRPASRAWV
                     VAALLLLVLTVALTRPLATERLAATPGVAIPDLVTRLAASAQADPGQGPATGEVRERL
                     AARYWTSFVAYPLSRLQTGSPVLAGSPPTAKAGILELLRRRIAGVNDWAVGRHGTERA
                     LVATTGVVYVLPFAFALAALAMVATCAQALLYLLCLAGLVAIPVAVDARRRRAVVTWW
                     LLPLAGAVALLVAGAMLSLLVLWLGETVHAADEEVGLLLAGSIGPVAAVVLAGRAARR
                     ARRLRRVAAVGGASS"
     gene            1567..>1804
                     /locus_tag="VEP73_12635"
     CDS             1567..>1804
                     /locus_tag="VEP73_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27317.1"
                     /translation="MSPSRELVPVPRPGGGRRGWRGWRGWRPRLDAAARLLCRVARSP
                     LARRLALAMLLAAAAAGIVGLSYATADAQAVPARP"
BASE COUNT          160 a          769 c          649 g          226 t
ORIGIN      
        1 accaccccgg ccgccagctc cgagccccac cccacccggt cgtcccgccg tccaccccct
       61 gacgacccgg gggccaccgt ccccacgggc ggcccccggc ccacagcgac cacgctccgc
      121 acccattacg cacctacagc gcttgccggt ccacgagggc aacgccccgg ccggcgagcc
      181 gacacaaccg aacagacgcg agcgcgagcg gctcgcggtc gcctccgccg caccccggag
      241 acggcccggc gcgacccgcg cggcgacggc gcccacaacc tggcgaccga tcgcccgccg
      301 gccgctccct ccgagccatc gcgctcccca tccccgaggg ggagccatgc ctgctgtcat
      361 gccccggcac gggtcgcggc ccgcgcaccg ttccggccgc ccccggcccg gctccgggcc
      421 gaccacccgg tcctggctgc ggaccgtgct cgtcccctac ctggccgcca cgttcgtcct
      481 cctcgcggtc gtgttcgcca tggccccgca ccgcaccggc gcgcccgcgt ccgggacgtg
      541 ggactaccag cgggaggtcg tcggcatcca cggcggcggg gccggcaacg cctacgcccg
      601 cgccgcgatc cgggcgacgg cgtggatgac caaggggtcg atcgagcgcc agcgtgacct
      661 gttccgcgtc aacctgctcg gcttcctggc cggccggccg ctggctggcg gggtcggcgc
      721 ctggcgggac gcccaccggc tgccgctcga aggcggcacg atcgccgtgc tcgtcggcct
      781 catcgcctac ggggtgctgc gccggccggc cagccgggcc tgggtggtgg ccgccctgct
      841 cctgctcgtc ctgaccgtgg ccctgacccg gcccctggcc accgagcggc tcgccgccac
      901 gcccggggtc gccatcccgg acctcgtcac gcgcctggcc gcgtccgccc aggccgaccc
      961 cgggcagggc ccggcgaccg gagaggtgcg cgagcgcctg gccgcccgct actggacgtc
     1021 gttcgtcgcc tacccgctgt cgcgcctgca gaccggcagc cccgtgctcg ccggctcgcc
     1081 gccgacggcc aaggccggca tcctcgagct gctccggcgc cggatcgccg gtgtcaacga
     1141 ctgggcggtc ggtcgccacg gcaccgagcg cgccctcgtc gccacgacgg gggtcgtcta
     1201 cgtgctgccg ttcgcgttcg cgctcgccgc gctcgcgatg gtcgcgacgt gcgcccaggc
     1261 gctgctctac ctgctctgcc tcgccgggct cgtggcgatc cccgtggcgg tcgacgcgcg
     1321 gcggcgccgc gccgtcgtca cctggtggct gctgccgctc gccggcgcgg tcgcgctgct
     1381 ggtcgccggc gcgatgctct ccctgctggt cctgtggctc ggcgagaccg tgcacgcggc
     1441 cgacgaggag gtcggcctgc tcctcgccgg gtcgatcggc ccggtcgcgg ccgtcgtgct
     1501 ggccggtcgc gccgctcgcc gcgcccgccg cctgcggcgg gtcgccgccg tgggaggtgc
     1561 gtcctcgtga gcccttccag ggagctcgtg ccggtgccga ggcccggcgg cggccggcgg
     1621 ggctggcgcg gctggcgcgg ctggcgaccg aggctcgacg ctgccgcgcg gctgctctgc
     1681 cgcgtggccc gcagcccgct ggcgcgccgg ctggcccttg ccatgctgct cgcggccgcg
     1741 gcggcgggca tcgtcggcct ctcgtacgcg accgccgacg cgcaggcggt cccggcccgg
     1801 ccgg
//
LOCUS       DASPYY010000809         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1681948, whole genome shotgun sequence.
ACCESSION   DASPYY010000809 DASPYY010000000
VERSION     DASPYY010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681948"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VEP73_12640"
     CDS             complement(<1..554)
                     /locus_tag="VEP73_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT27318.1"
                     /translation="MTPMTPTRIGFVGAGAVATRHVRTLAGFDDVQVVAVADPAVERA
                     GRLADEVGATAYERWRPMLERERLDALYVCIPPFAHGPPELAAVEAGLPLFVEKPLAI
                     DAGTAESIAERIEGTGLLTCAGYHWRWLDVFERARALLDGNPARLAQCHWLDKVPPPA
                     WWVRRDGSGGQTIEQTTHVLDLARA"
     gene            722..>1826
                     /locus_tag="VEP73_12645"
     CDS             722..>1826
                     /locus_tag="VEP73_12645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYT27319.1"
                     /translation="MSRRARRPAASRTLLVVVAAALALASVLALRWAGGRPAPSATGP
                     ASATGPAASGTVPPGGTRVAEGTADEVHWSFTGARSVAFDWRGTASEVRFGRTPAYGG
                     TAAGRAPDPVPPSSPGPFREALLDGLEPGTTYHYSIGGQPDRTFTTAPTGPFRFDVEA
                     DIGDSATYPAVAEVQRMIAADRPAFVIASGDLSYANDHGPEAVDRHFNDVMAWSRDAA
                     YMPAWGNHEWDSGGDDLRNYKGRFAIANAAASPGAPGLGCCGEDWGWFDAGGVRFLSY
                     PEPYRGAWKAWEVQARRVMAAAQADPAVRFIVTYGHRPAYSTGHHPGDEDLAAILDRL
                     GDAYPKYKLNLNGHSHDYERFSPIHGVTHLTVTG"
BASE COUNT          225 a          702 c          678 g          221 t
ORIGIN      
        1 gcccgggcga ggtcgaggac gtgggtggtc tgctcgatgg tctggccgcc cgacccgtcc
       61 cggcgcaccc accaggccgg cggggggacc ttgtcgagcc agtggcactg ggccagccgg
      121 gccgggttgc cgtcgagcag cgcccgggcc cgctcgaaga cgtcgagcca gcgccagtga
      181 tagcccgcgc acgtcaggag accggtgccc tcgatccgct ccgcgatcga ctcggccgtc
      241 ccggcgtcga tggcgagcgg cttctccacg aagaggggca gccccgcctc gacggccgcg
      301 agctcgggcg gcccgtgggc gaacggcggg atgcacacgt agagggcgtc gaggcgttcc
      361 cgctcgagca tcggacgcca acgctcgtag gcggtggccc cgacctcgtc ggcgagcctg
      421 ccggcgcgct cgaccgcggg gtcggcgacc gccacgacct ggacgtcgtc gaagccggcg
      481 agcgtccgga cgtggcgggt ggccaccgcg cccgcgccga cgaagccgat cctggtgggc
      541 gtcatgggag tcatgggcga tggatcgcca gcgagccggt acgccaatca ccccggtccg
      601 ccggagcgcc ccgccgacgg cacggggcgc agccgacgca gggccgccac ggcgtttgcg
      661 ggagcgaggc gggcgggttc gggcggtacg atccggtccg gtccactcgt ctggaggctc
      721 catgtcccgc cgtgcccgcc gccccgccgc ctcccgcacg ctcctggtcg tcgtggcagc
      781 cgccctggcc ctcgcgtccg tgctggccct ccgctgggcg ggcggccggc cggcgccgtc
      841 cgcgaccggc ccggcgtccg cgaccggccc ggccgcgtcc gggacggtcc cgccgggcgg
      901 gacccgggtg gccgagggga ccgccgacga ggtccactgg tcgttcacgg gcgcccggtc
      961 ggtcgccttc gactggcggg gcaccgcctc cgaggtgcgc ttcggccgca ccccggccta
     1021 tggcgggacg gcggccggcc gggcgccgga cccggtgccg ccctcctccc ccgggccgtt
     1081 ccgggaggcg ctgctcgacg ggctggagcc cgggacgacc taccactact cgatcggggg
     1141 ccagccggac cggacgttca ccaccgcgcc caccggcccg ttccgcttcg acgtcgaggc
     1201 cgacatcggc gactcggcga cctacccggc ggtggcggag gtccagcgca tgatcgccgc
     1261 cgaccggccg gccttcgtga tcgcctccgg ggacctgagc tacgcgaacg accacggccc
     1321 cgaggccgtg gaccggcact tcaacgacgt catggcctgg agccgggacg ccgcctacat
     1381 gccggcgtgg ggcaaccacg agtgggactc cggaggcgac gacctgcgca actacaaggg
     1441 ccgcttcgcc atcgcgaacg cggcggcctc gcctggcgcg ccagggctcg gctgctgcgg
     1501 cgaggactgg ggctggttcg acgccggggg cgtgcgcttc ctctcctatc ccgagccgta
     1561 ccggggtgcc tggaaggcgt gggaggtcca ggcgcggcgg gtcatggcgg ccgcccaggc
     1621 ggacccggcg gtccgcttca tcgtcaccta cggccaccgg cccgcctact cgaccggcca
     1681 ccacccggga gacgaggacc tggccgcgat cctcgaccgc ctgggcgacg cctatcccaa
     1741 gtacaagctg aacctgaacg gccactccca cgactacgag cgcttctccc cgatccatgg
     1801 cgtgacccac ctgacggtca ccggtg
//
LOCUS       DASPYY010000810         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_277355, whole genome shotgun sequence.
ACCESSION   DASPYY010000810 DASPYY010000000
VERSION     DASPYY010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277355"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1324
                     /locus_tag="VEP73_12650"
     CDS             44..1324
                     /locus_tag="VEP73_12650"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYT27320.1"
                     /translation="MTRFASVPEAAAAVAAGEMVVVVDDADRENEGDLIMAAEAATPE
                     KIAFFVRHTSGVIVMPMLGERLDELELPLMVAVNTEAHRTQFTVSVDARNGVTTGISA
                     ADRATTIAALIDPATRPGDLARPGHIFPLRYREGGVLKRAGHTEAGVDLARLAGRYPA
                     AVLCEIVNEDGTMARLPDLERFAERHGLRLISIADIIRHRRQREKLVRRVAEARIPTR
                     WGEFTAYGYESVLDGEQHLALVRGAVQGERDVLVRVHSECLTGDVFGSLRCDCGPQLD
                     GALRRIAEEGLGAVVYLRGHEGRGIGLAHKLAAYNLQEQGRDTVDANLELGLPVDDRE
                     YGIGSQILVDLGVTTMRLLTNNPAKYTGLGGFGLTIVERVPLETHPTAENLHYLRTKR
                     ERMGHLLDLPDDLVTEVSTGTEGQADKGARGAPG"
     gene            1362..1835
                     /gene="ribE"
                     /locus_tag="VEP73_12655"
     CDS             1362..1835
                     /gene="ribE"
                     /locus_tag="VEP73_12655"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYT27321.1"
                     /translation="MGKVLEGSLDGTGRRVAIAASRFNRLVTDLLVAGAAEALRRHGV
                     AEADVDVAWAPGSFELPLVAARLAATGRYAGVVALGAVVRGETAHFDHVAGQAASGLA
                     AVARSSGVPVAFGVLTTDTMAQALDRAGGKAGNKGAEAALGVLEMAGLLEAIDKG"
     gene            1872..2747
                     /gene="hisG"
                     /locus_tag="VEP73_12660"
     CDS             1872..2747
                     /gene="hisG"
                     /locus_tag="VEP73_12660"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYT27322.1"
                     /translation="MLKLVLPKGSLERATMALFEAADLPVKRGSERDYHGSIDDPRVE
                     RVSVLRPQEIPRYVEEGFFDLGITGRDWVEETGSKVATLAELPYSKQTANPVQVVLAV
                     HRDLGVTRPEELQPGLRVSTEFPNLTRRYFAELGVPARVFPSYGATEAKVPEIVDAVV
                     DLTETGSTLRSHGMVVIATLLTSFTLVVANLESAADAARRQAMDDLVTLLVGAATARS
                     KTLIKLNVASADLPGVLGVLPAMKAPTVTALSDSGFHAVETVVDKSTVNHLIPTLKAA
                     GAFDILELPIAKIVA"
     gene            2939..3142
                     /locus_tag="VEP73_12665"
     CDS             2939..3142
                     /locus_tag="VEP73_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYT27323.1"
                     /translation="MPEGTVKWFSNEKGYGFIARNGGDDVFVHFSAIQIDGYKTLAEG
                     QAVEFEITDGPKGPQASNVRPLA"
     gene            complement(3337..3792)
                     /locus_tag="VEP73_12670"
     CDS             complement(3337..3792)
                     /locus_tag="VEP73_12670"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HYT27324.1"
                     /translation="MSDPLDHARAADDATAAAAQRDALEQMREQLAAVSAGDAVAEAA
                     VPLVTLAYMRLGVPPGQNDRYRDLEAASLLIDALAGLLDAVKGRLGAVEGELRGALAD
                     LQLGYADVLRHLGGHAGPGGAGPAAPQRPPPPPAEPGSRPSGLWVPGQP"
     gene            4124..>4707
                     /gene="infC"
                     /locus_tag="VEP73_12675"
     CDS             4124..>4707
                     /gene="infC"
                     /locus_tag="VEP73_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYT27325.1"
                     /translation="MGADGEQLGVMDTVTAQRMASEQDLDLVEVAPLARPPVCKLMDY
                     SKYKYEQAIKTKEARRKQSLVVLKEMKMRPKIDPHDYETKKGHVERFLKQGHKVKVTI
                     MFRGREMAHTELGRRLLERLATDITEIAQVDSPARQDGRNMIAVFSPRKSGAGGPRAA
                     GPSDRGAAARGAAQGDRGGSQAARSTTPANQAGGR"
BASE COUNT          707 a         1680 c         1726 g          594 t
ORIGIN      
        1 cgtggaccgg gcaccgcgcg tgcatggcgg gaggccgggg ccgatgacca ggttcgcgag
       61 cgtccccgag gcggccgccg ccgtcgcggc cggcgagatg gtggtcgtgg tcgacgacgc
      121 cgaccgcgag aacgagggcg acctcatcat ggccgccgag gcggccacgc ccgagaagat
      181 cgccttcttc gtgcgccaca cctccggcgt gatcgtcatg cccatgctcg gcgagcgcct
      241 cgacgagctc gagctgcccc tgatggtcgc ggtcaacacc gaggcgcacc ggacgcagtt
      301 caccgtgtcg gtcgacgccc gcaacggggt gacgaccggc atctcggcgg ccgaccgggc
      361 caccacgatc gcggcgctga tcgacccggc cacccgcccc ggcgacctcg cccgccccgg
      421 gcacatcttc ccgctccgct accgggaggg cggggtgctc aagcgggccg gccacaccga
      481 ggcaggcgtc gacctggccc gcctggccgg ccgctacccg gccgccgtgc tctgcgagat
      541 cgtcaacgag gacgggacca tggcccggct gcccgacctc gagcggttcg ccgagcgcca
      601 cggcctcagg ctcatctcga tcgccgacat catccgtcac cggcgccagc gcgagaagct
      661 cgtcaggcgg gtcgccgagg cgcgcatccc cacccgctgg ggagagttca ccgcctacgg
      721 ctacgagtcg gtgctcgacg gcgagcagca cctcgcgctc gtcagggggg cggtccaggg
      781 ggagcgggac gtgctcgtgc gggtccactc cgagtgcctc accggcgacg tgttcggcag
      841 cctgcgctgc gactgcggcc cgcagctgga cggggcgctg cgccggatcg ccgaggaggg
      901 gctcggggcc gtcgtctacc tccgcgggca cgagggccgc ggcatcgggc tggcccacaa
      961 gctcgccgcc tacaacctgc aggagcaggg ccgggacacg gtcgacgcga acctcgagct
     1021 gggcctgccc gtggacgacc gcgagtacgg gatcggctcg cagatcctcg tcgacctcgg
     1081 cgtcaccacg atgcggctgc tcaccaacaa cccggccaag tacaccggcc tcggcggctt
     1141 cggcctcacg atcgtcgagc gggtgcccct cgagacccac cccaccgccg agaacctcca
     1201 ctacctgcgg accaagcgcg agcgcatggg gcacctgctc gacctgcccg acgacctggt
     1261 caccgaggtc tcgaccggga cggaggggca ggccgacaag ggggctcgcg gcgcgccggg
     1321 ctagcccctc cggggcccga cggacgagga ggaggacgct catgggcaag gtgctggagg
     1381 gaagcctcga cgggaccggc aggagggtcg cgatcgccgc gagccgcttc aaccggctgg
     1441 tgaccgacct gctcgtggcc ggggccgcgg aggcgctgcg ccgccacggc gtggccgagg
     1501 ccgacgtgga cgtcgcctgg gcgcccggat cgttcgagct gccgctcgtc gcggccaggc
     1561 tcgcggcgac cgggcggtac gcgggcgtgg tcgcgctcgg cgccgtcgtc cgcggcgaga
     1621 ccgcccactt cgaccacgtc gccgggcagg cggcctccgg cctggccgcg gtcgcgcggt
     1681 cgagcggcgt cccggtcgcg ttcggcgtgc tgaccaccga caccatggcg caggcgctcg
     1741 atcgggcggg cggcaaggcg ggcaacaagg gggccgaggc ggccctcggc gtccttgaga
     1801 tggccggcct gctcgaagcg atcgacaagg gatgagggga cggggatgag gggaccgggg
     1861 agggcccgac cgtgctgaag ctggtgcttc cgaagggcag cctggagcgt gccaccatgg
     1921 ccctgttcga ggccgccgac ctgccggtca agcggggcag cgagcgcgac taccacggct
     1981 cgatcgacga cccgagggtc gagcgggtct cggtgctgcg gccccaggag atcccccggt
     2041 acgtcgagga ggggttcttc gaccttggca tcaccggccg ggactgggtc gaggagaccg
     2101 gcagcaaggt cgccaccctg gccgagctgc cctactccaa gcagacggcc aacccggtcc
     2161 aggtggtgct cgccgtccac cgcgacctcg gcgtcacccg gcccgaggag cttcagcccg
     2221 gcctgcgggt gtcgaccgag ttcccgaacc tcaccaggcg gtacttcgcc gagctcgggg
     2281 tgcccgcccg ggtcttcccc tcctacgggg cgaccgaggc caaggtcccc gagatcgtcg
     2341 acgcggtggt cgacctgacc gagaccggct cgacgctgcg gagccacggc atggtggtga
     2401 tcgccaccct gctcacctcg ttcaccctgg tggtcgcgaa cctcgagtcg gccgctgacg
     2461 cggcccgccg ccaggccatg gacgacctcg tcacgctgct ggtcggcgcg gccacggccc
     2521 gctccaagac cctcatcaag ctcaacgtgg ccagcgccga cctgccaggc gtgctcggcg
     2581 tcctgccggc catgaaggcg ccgaccgtca ccgcgctgtc ggactccggc ttccacgcgg
     2641 tcgagacggt ggtcgacaag tcgacggtca accacctcat cccgaccctc aaggcggccg
     2701 gcgcgttcga catcctcgag ctgcccatcg ccaagatcgt cgcctagccg cgccggccgc
     2761 ccggccggcg cctccagtcc ggtttccacc actgagccgg acgccccggt acgacgtcaa
     2821 gggcgggtct gtcgcgttgt tgtgccgctg cggtcgctga gttacattcc gcgcgcccga
     2881 ccggaattcc ccggcgcccg tgtccggggg cggtcggggc gcgacaggag ggacagagtt
     2941 gccagaaggc accgtcaagt ggttcagcaa cgagaagggc tacgggttca tcgcccgtaa
     3001 cggaggcgac gacgtcttcg ttcacttctc ggcaatccag atcgacggct acaagaccct
     3061 tgccgagggc caggccgtcg agttcgagat cacggacggc cccaaggggc cgcaggcctc
     3121 gaacgtccgg ccgcttgcgt agaggagcag gagctcgacg ggccttcggt tcgtaggact
     3181 tccggcagca cgcaagaccc gcgagggccc cgccaaacga cggcggggcc cttccgctcg
     3241 cctgccggaa ccggcgaggg gaaatgcacg ccgctatgac agccgcaatt ccggcgacga
     3301 gcggttggcc cggtgtggcg tgacagcccc ggaggctcag ggctggccgg gcacccagag
     3361 gcccgagggc cgggagccgg gctcggcggg aggcggtggc ggccgctgcg gcgcggccgg
     3421 gccggcgccc ccgggcccgg cgtggccgcc caggtgccgc agcacgtcgg cgtagccgag
     3481 ctgcaggtcg gcgagggcgc cgcgcagctc gccctcgacc gcaccgagcc ggcccttgac
     3541 cgcgtcgagc aggccggcca gcgcgtcgat caggaggctc gccgcctcga ggtcgcgata
     3601 ccggtcgttc tggccgggtg ggacgccgag ccgcatgtac gcgagggtca cgaggggaac
     3661 ggcggcctcg gcgacggcgt cgccggccga caccgcggcg agctgctcgc gcatctgctc
     3721 gagcgcgtcg cgctgcgccg ccgcggcggt cgcatcgtcg gcggctcgag cgtggtcgag
     3781 ggggtctgac atgggacgtt ctcctggtgt agccttgggt gtagttcggg ctggtgcgcc
     3841 ttgcgtctgc ggctcgagcg ttgggtgggt ctcgaaccga caggtcacgt cgaagtacag
     3901 gcaacaccgt taggatacgt gcatacagcg aagcagaagc gagggcttct cacctccagg
     3961 ccggtcgggc ccatcggagg tcgacgacag accaccggct caggtggtgg gcggcccttg
     4021 gccgcctttc gttgtttccg ggcgtggaag cccagaggag ggagacagca tcagcgtaga
     4081 acccaggatc aacgaccgga tccgggccgc acaggtccgg gtcgtgggtg ccgacggaga
     4141 gcagctcggc gtcatggaca ccgtcaccgc ccagcggatg gcaagcgagc aggacctcga
     4201 cctcgtcgag gtggcgccgc tggcccggcc gccggtctgc aagctgatgg actactcgaa
     4261 gtacaagtac gagcaggcca tcaagaccaa ggaagcccgc cgcaagcaga gcctggtggt
     4321 cctcaaggaa atgaagatgc ggcccaagat cgacccgcac gactacgaga ccaagaaggg
     4381 tcacgtcgag cggttcctca agcagggcca caaggtcaag gtcacgatca tgttccgcgg
     4441 tcgcgagatg gcgcacaccg agctcggccg tcgcctgctc gagcggctgg cgaccgacat
     4501 caccgagatc gcccaggtcg acagcccggc gcggcaggac ggccgcaaca tgatcgcggt
     4561 cttctcgccc cgcaagagcg gcgccggcgg ccccagggcg gccggcccgt ccgaccgggg
     4621 cgcggcggcc cggggcgcgg cgcagggcga ccgtggcggg tcccaggcgg cccgctcgac
     4681 caccccggcc aaccaggctg gcgggcg
//
LOCUS       DASPYY010000811         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_840393, whole genome shotgun sequence.
ACCESSION   DASPYY010000811 DASPYY010000000
VERSION     DASPYY010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840393"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VEP73_12680"
     CDS             <1..326
                     /locus_tag="VEP73_12680"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYT27326.1"
                     /translation="QEHERRYGYRDPDAEVELVNVRVALVVEGPSPEPEAAPAGRLER
                     STRQARLGGEWLEAEILTGEPEEGFATEGPCVFELPEATLVLPPGWQAGVDAHGTIRA
                     ERSGG"
     gene            323..>1774
                     /locus_tag="VEP73_12685"
     CDS             323..>1774
                     /locus_tag="VEP73_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYT27327.1"
                     /translation="MIDPVTLQVLVGALRAACEEMGAVLVRAAHSANIKERRDCSTAL
                     FDAAGELVMQAEHIPVHLGSMPDAVAAVLGEEHRPGDVWILNDPYQGGTHLPDITLIS
                     PIFTDEELLGFAASRAHHADVGGPTPAGMPARSTRLEDEGVVIPPTRANDDRLARLAA
                     EMRNPPQRLADLRAQRAANLTGERRLLELAGKHGPEVLRSGMAEILDYAERRTRAALA
                     ELPDGAYEAEDLMEDDAVGEPRDIRLRVTATIDGQRLTLDFTGTDSQVAGNLNCPLPV
                     TKSASFFAVRVLTDPDAPPSAGAFRPVEVIAPEGCLLHARPPAAVAAGNVETSSRVAD
                     LVIEALAGATPAPAQGQGTMNNLTLASKGSGRDAERRRDRQVAGDWTYYETVGGGQGA
                     CPEADGPSAVHVAMSNTLNTPVEALEAEFPLRVRELSVRRGSGGAGRHRGGDGIVREL
                     EALAPMSFTLIAERRRHAPRGRDGGADGKPGRNL"
BASE COUNT          253 a          670 c          645 g          206 t
ORIGIN      
        1 agcaggagca cgagcgccgc tacggctatc gcgaccccga cgccgaggtc gagctcgtga
       61 acgtgcgcgt ggcgctggtc gtggagggcc catcgcccga gcccgaggcg gcgccggccg
      121 ggcgcctgga gcggtcgacc cgccaggccc gcctgggcgg cgaatggctc gaggccgaga
      181 tcctgaccgg ggagccggag gaggggttcg cgacggaggg cccatgcgtg ttcgagcttc
      241 ccgaggcgac cctcgtcctg ccgcccggct ggcaagccgg ggtggacgcg cacggaacga
      301 tccgcgccga gcggagcggc ggatgatcga cccggtcacg ctccaggtcc tggtcggcgc
      361 gttgcgagcc gcctgcgagg agatgggcgc ggtgctcgtg cgcgctgccc actcggcgaa
      421 catcaaggag cggcgcgact gctcgacggc gctgttcgac gccgccgggg agctggtgat
      481 gcaggcggag cacatcccag tccacctcgg ctcgatgccc gacgcggtcg ccgccgtgct
      541 cggcgaggag caccggcccg gcgacgtgtg gatcctcaac gacccctacc aggggggcac
      601 tcacctgccc gacatcaccc tgatctcgcc gatcttcacc gacgaggagc tgctcggctt
      661 cgccgccagc cgcgcccatc acgcggacgt cggcggaccg accccggcag ggatgccggc
      721 ccgctccacc cggctcgagg acgagggcgt ggtgatccca cccacccgcg ccaacgacga
      781 tcggctggcg cgcctggccg ccgagatgcg caacccgccg cagcgcctcg ccgatctgcg
      841 agcgcagcgg gccgccaacc tgaccggcga gcgtcgcctg ctcgagctgg ccggcaagca
      901 cgggcccgag gtgcttcgct ccgggatggc ggagatcctc gactacgccg agcgccgcac
      961 gcgcgcagcg ctcgccgagc tgcccgacgg cgcctacgag gcagaggacc tgatggagga
     1021 cgacgccgtc ggcgagccgc gcgacatccg gctgagggtc acggcgacga tcgacggtca
     1081 gcgcctgacg ctcgacttca ccggcaccga ttcccaggtc gccggcaacc tcaattgccc
     1141 cctgccggtc accaagtcgg cctccttctt cgccgtccgc gtgctcaccg accccgacgc
     1201 gcccccctct gccggtgcct tccgtccggt cgaggtgatc gctcccgagg gctgtctgct
     1261 gcacgcgcgg ccgcccgccg cggtcgccgc cggcaacgtg gagacgtcca gccgggtggc
     1321 cgacctggtg atcgaggccc tcgcgggcgc gacgccggcg cccgcccagg gccaggggac
     1381 gatgaacaac ctgaccctgg ccagcaaggg gtctgggcgc gacgccgaac ggcgacgcga
     1441 caggcaggtg gcgggcgatt ggacctacta cgagacagtc ggcggcggcc agggcgcctg
     1501 ccccgaggcc gacgggccca gcgccgtcca cgtggccatg tcgaacaccc tcaacacgcc
     1561 ggtcgaggct ctggaggccg agttcccgct tcgtgtgcgc gagctgtccg tacgcagggg
     1621 cagcggaggc gctgggcggc accgcggcgg cgacgggatc gtgcgcgagc tagaggccct
     1681 cgccccgatg agcttcaccc tgatcgccga gcgccgccgc cacgcccccc gagggcgcga
     1741 cggcggcgcc gacggcaaac ccggccgcaa cctg
//
LOCUS       DASPYY010000812         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_415673, whole genome shotgun sequence.
ACCESSION   DASPYY010000812 DASPYY010000000
VERSION     DASPYY010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415673"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VEP73_12690"
     CDS             <1..647
                     /locus_tag="VEP73_12690"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HYT27328.1"
                     /translation="RSWERMGGLPLFVLVAVAGLVAGYVRGGSAEGLRETAVRGRSLA
                     LAGLAVQAVIGAPLLRALGLPRVLGGVLLALALVVLVGVARANGRLPGVPLLALGLLL
                     NLLVTLANLGVPVSAATLRRADVAVEQPVPERPDLKHVLAGPASRIPVLGDELAVPPL
                     RTVTSVGEVAEFAGLFLLIQHMMLMGAVPGRRERDGDGQSAHEARTIPWSTWAD"
     gene            complement(676..972)
                     /locus_tag="VEP73_12695"
     CDS             complement(676..972)
                     /locus_tag="VEP73_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT27329.1"
                     /translation="MVRRTAGPSEPIGAVSRGPDKRKGRTVAAPRKASSRIETASYLR
                     TAEVADLLHVSPKTVSRWAKEGKLPFLKTLGGHRRYPEAKIRELADELREEPTG"
     gene            complement(1162..2103)
                     /locus_tag="VEP73_12700"
     CDS             complement(1162..2103)
                     /locus_tag="VEP73_12700"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT27330.1"
                     /translation="MVAGGAGFLGASLVDRLQAEGDEVIVIDDLSHGHLANLSEARRR
                     GGIRFHRMDVGQGGLSTLAGRIRADAWVHLAMTADPVRAWSTPSADARAVVAGTVEIL
                     EAARETGGARVVVASSGAYLFPAQLTGLSKPDDRPAPFHPLGAGRLASEAYAGAYGAQ
                     GLEVAVLALGTVYGPRSDPLGPGLVARAAWSMLQGHPPRVEGDGRQARDWLFVDDAVD
                     ALARAVHSRATGRLHVGTGTATPVLDVVERLAARIGWSGEPEWVPARPGDPRRSALDT
                     SLAGEVLSWQPWTGLDDGLQPTIDWFEARLPARHRYT"
BASE COUNT          269 a          807 c          778 g          308 t
ORIGIN      
        1 cgaggagctg ggagcgcatg ggtggactac cgctgttcgt cctggtcgcc gtggcgggcc
       61 tggtcgccgg ctacgtccgg ggcgggagcg ccgagggcct ccgtgagacc gccgtccggg
      121 gccgctcgct ggccctcgcc ggcctggccg tccaagcggt gatcggcgcg cccctgctgc
      181 gggcgctcgg cctcccccgc gtcctcggcg gcgtcctgct cgccttggcc ctcgtcgtcc
      241 tggtgggcgt ggcgcgcgcc aacgggcggc tccccggggt ccccctgctc gctctcgggc
      301 tgctgctcaa cctgctggtg accctggcca acctgggggt gccggtgtcg gcggcgaccc
      361 tgcgcagggc ggacgtggcc gtggagcagc cggtgcccga gcggcccgac ctgaagcacg
      421 tcctcgcggg gccggcgtcc cgtatcccgg tgctcggcga cgagctcgcc gtgccgccgc
      481 tgcggacggt gacgagcgtc ggcgaggtcg ccgagttcgc cgggctgttc ctgctcatcc
      541 agcacatgat gctcatgggc gccgtgcccg ggcgacgcga gcgcgacggg gacgggcaga
      601 gcgcccacga ggcgaggacg atcccgtggt cgacctgggc cgactgaccc gggccgacca
      661 gggaacgtcc cgggcttagc cggtcggctc ctcgcggagc tcgtccgcca gctcccggat
      721 cttggcctcc gggtagcgcc ggtgaccacc gagggtcttc aggaagggca gctttccttc
      781 cttcgcccat cgcgacacgg tcttcggtga gacgtgcagg agatcggcga cctctgcggt
      841 acgcagataa ctggccgtct cgatccttga gcttgccttg cgcggagctg ccaccgtgcg
      901 tccctttctc ttgtccggac ctcggctcac ggcgccgatt ggctcggatg gaccggctgt
      961 tcgtcggacc acgacagtcg cggacgtgcg gcggatacgg ctctgtcggt caccctgccg
     1021 gatgcagaac gaacaaggca attatcgaga ccggcccgag cgccaaccat agcccgcttg
     1081 ggccgtttca tccgccgagg ttacctaaag cacgttccct acacagcccg ttcactactg
     1141 agccccggcc cttctcagcg ctcaagtgta ccgatgcctg gccggcaatc tcgcctcgaa
     1201 ccagtcaatc gtcggctgga gcccgtcgtc gagccctgtc cacggctgcc agctgagcac
     1261 ctcgccggcc aggctcgtgt caagggccga gcggcgcggg tcgcccgggc gggccggtac
     1321 ccactccggc tcgccgctcc agccgatgcg cgcggcgagg cgctccacca cgtcgagcac
     1381 tggcgtggcg gtccccgtgc cgacgtggag ccgtccggtc gccctcgagt gcaccgcccg
     1441 ggcgagcgcg tcgaccgcgt cgtccacgaa cagccagtcg cgggcctggc ggccgtcgcc
     1501 ctcgacccgg ggcgggtggc cctggagcat cgaccaggcg gccctggcta ccagcccggg
     1561 cccgagcggg tcggagcgcg gcccgtacac ggtcccgagg gcgaggacgg ccacctcgag
     1621 cccctgggcg ccgtaggcgc cggcgtaggc ctcggacgcc aggcgccccg ccccgagggg
     1681 atggaagggc gccggccggt cgtccggctt gctcaggccc gtgagctggg cgggaaacag
     1741 gtaggcgccc gacgacgcga cgaccacgcg ggcgccgccc gtctcgcggg ccgcctccag
     1801 gatctcgacg gtgcccgcga ccaccgcgcg ggcgtcggcg ctcggcgtcg accacgcccg
     1861 gacggggtcg gcggtcatgg cgagatgcac ccaggcgtcg gcgcggatcc ggccggcgag
     1921 ggtggacagg ccgccctggc cgacgtccat ccggtggaag cggatcccgc cgcgccgtct
     1981 ggcctcgctc aggttggcga gatggccgtg ggagaggtcg tcgatgacga tgacctcgtc
     2041 cccctcggcc tgcagccggt cgaccaggct ggccccgagg aacccggccc cgccggccac
     2101 catcaccaac acgcgcgccc tcctcgacga tcaagcgcat cctatccaga ggaggtcaac
     2161 cc
//
LOCUS       DASPYY010000813         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1402309, whole genome shotgun sequence.
ACCESSION   DASPYY010000813 DASPYY010000000
VERSION     DASPYY010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402309"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VEP73_12705"
     CDS             complement(<1..754)
                     /locus_tag="VEP73_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT27331.1"
                     /translation="MSAAGLDRWRGVAAEQADDVPSGVSALLRSRSRRLLGSLLRPHR
                     RLLCLLAVVVGVENGAAMAGPYLVKVAIDRGIPPLARGGSARPLAVIVALLVAAAVVQ
                     AVTLRYFLVGTGRLGQRLLYDLRTRVFDHFQRLSLAFHERYTSGRVISRLTSDMDAIA
                     EVLDGGLEEVVTALLSVVSIAVIILLLDRPLGLVTLCVFPLLALLTRWFRRNSAVAYR
                     RAREAVALVIVWFTESLGGIRAVQAFRREQRNQ"
     gene            complement(751..>2368)
                     /locus_tag="VEP73_12710"
     CDS             complement(751..>2368)
                     /locus_tag="VEP73_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT27332.1"
                     /translation="PLCLLALALGAAEALLTFVRRWAQGSAAQGVEADMRSDLYRHLQ
                     RLPVAFHDRWQSGQLLSRAMTDLSVIRRFVGFGLTFLIVNALTFITVEILLLRLYAPL
                     GLVVLASALPVVWLSARFERAYVGVSRRVQDQLGDLTTAVEESATGIRVIKAFGRREL
                     VGERFGEHARLLYGSSMDSVHLRARLWSLFDLIPSLTLAVVLFAGALAVARGSLTLGG
                     LVAFMSLELLLVWPVAALGFILASGWEAATAAERVYEVLDTRPDVVDRPGAVALARAR
                     GHLRFEGVGFRYPGSDRLLLHDVTLDVRPGETVALVGATGSGKTTLASLVPRLYDVCE
                     GRITLDGADLRDLRLASLRRHVAAAFEEPILFSASVRENLLLGTRDASDAEIAEALAV
                     ARAEFVLDLPWGLDTRIGEQGLSLSGGQRQRLALARAVIVRPRVLVLDDPLSALDVHT
                     EALVEAALARVLRGTTGLVVVHRPSTLALADRVALLEDGTITAVGAHGELMATVPSYR
                     AILSQEAEPQEAEPPAGHGRRRDDGEARAS"
BASE COUNT          365 a          822 c          951 g          230 t
ORIGIN      
        1 cctggttgcg ctgctcacgc cggaacgcct ggacggcccg gatcccgccg agcgactcgg
       61 tgaaccagac gatgacgagc gcgaccgcct cgcgggcccg ccggtaggcg accgccgaat
      121 tccgccggaa ccagcgggtg agcaaggcga gcagcgggaa cacgcagagg gtgacgaggc
      181 cgagcggccg gtcgagcagc aggatgatca ccgcgatgct gacgaccgag agcagcgccg
      241 tgacgacctc ctcgaggccg ccgtcgagga cctccgcgat ggcgtccatg tcggaggtga
      301 ggcgcgagat cacccgcccg gaggtgtagc gctcgtggaa cgcgaggctc aggcgctgga
      361 agtggtcgaa gacccgggtg cgcaggtcgt acaggagccg ctggccgagc cggccggtgc
      421 cgaccaggaa gtaccgcaag gtcaccgcct gcacgaccgc ggcggcgacc agcagcgcga
      481 cgatcacggc caggggccgg gcgctcccgc cgcgcgcgag cggcgggatg ccgcggtcga
      541 tggcgacctt caccaggtac gggccggcca tggccgcgcc gttctcgacg ccgacgacca
      601 cggcgagcag gcagagcagc cgccggtggg ggcgcagcag cgagccgagg agccgccggc
      661 tgcgcgagcg cagcagcgcg gacacgccgc tcggcacgtc gtcggcctgc tcggcggcga
      721 ccccgcgcca tcggtcgagg ccggccgcgc tcacgatgcc ctcgcctcgc cgtcgtcgcg
      781 ccgccgcccg tggccggcgg gcggctcggc ctcctggggc tcggcctcct gggagaggat
      841 cgcccggtag gacggcaccg tggccatgag ctcgccgtgg gcgccgaccg cggtgatcgt
      901 gccgtcctcg agcagcgcca cccggtccgc cagcgccagc gtcgacggcc ggtggacgac
      961 cacgagcccg gtggtgccgc gcaggacgcg cgccagcgcc gcctcgacga gcgcctcggt
     1021 gtgcacgtcg agggctgaca gcgggtcgtc caggacgagg acgcggggcc ggacgatgac
     1081 cgcgcgggcg agcgcgagcc gctgccgctg cccgcccgac aggctgaggc cctgctcgcc
     1141 gatgcgggtg tccagccccc acggcaggtc gagcacgaac tcggcccgcg cgacggcgag
     1201 cgcctcggcg atctcggcgt cggacgcgtc ccgcgtgccg agcaggaggt tctcgcgcac
     1261 gctggccgag aacaggatcg gctcctcgaa cgcggcggcg acgtggcggc ggagcgaggc
     1321 gaggcgcagg tcgcgaaggt cggcgccgtc gagggtgatg cggccctcgc agacgtcgta
     1381 gaggcgcggc acgagcgagg ccagcgtggt cttgcccgac ccggtcgcgc cgaccagcgc
     1441 gacggtctcg cccgggcgca cgtcgagcgt gacgtcgtgg aggaggaggc ggtcgctgcc
     1501 cgggtagcgg aacccgacgc cctcgaagcg gaggtgcccg cgggcgcggg ccagcgcgac
     1561 cgcgccgggc cggtcgacga cgtcgggccg ggtgtcgagg acctcgtaca cgcgctcggc
     1621 cgcggtggcc gcctcccagc cgctcgcgag gatgaagccg agcgccgcga cgggccagac
     1681 gagcagcagc tcgagtgaca tgaacgcgac caggccgccg agggtgaggc tgccgcgggc
     1741 gacggcgagc gcgccggcga agagcacgac ggcgagggtg aggctcggga tgaggtcgaa
     1801 cagcgaccac agccgggcgc ggaggtggac gctgtccatg gacgagccgt acagcaggcg
     1861 ggcgtgctcg ccgaagcgct cgccgaccag ctcccgccgg ccgaacgcct tgatgacccg
     1921 gatgccggtc gcggactcct cgaccgcggt ggtgaggtcg ccgagctggt cctggacccg
     1981 ccgcgagacg ccgacgtagg cgcgctcgaa gcgcgcggag agccagacga cgggcagggc
     2041 cgaggcgagg acgaccagcc cgaggggcgc gtagaggcgg aggagcagga tctccacggt
     2101 gatgaaggtg agcgcgttca cgatgaggaa ggtcagcccg aagccgacga accgccggat
     2161 gaccgagaga tcggtcatgg cacgcgacag gagctggccg gactgccagc ggtcgtggaa
     2221 ggcgaccggc agccgctgca ggtgccggta gaggtcgctg cgcatgtcgg cctccacgcc
     2281 ctgcgcggcg gacccctggg cccagcggcg cacgaacgtg agcagtgcct cggccgcgcc
     2341 gagggcgagg gcgagcaggc agaggggc
//
LOCUS       DASPYY010000814         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1823014, whole genome shotgun sequence.
ACCESSION   DASPYY010000814 DASPYY010000000
VERSION     DASPYY010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823014"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VEP73_12715"
     CDS             <1..315
                     /locus_tag="VEP73_12715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT27333.1"
                     /translation="EAKLAIARAVEARFFAVLADGRVVSYADLYRDGRTAQVEDVATL
                     EAHRGRGYASAVVLRAVEAARHAGCDLVFLVADADDWPKELYRRLGFDDLGRYVKFIR
                     PA"
     gene            complement(422..1690)
                     /locus_tag="VEP73_12720"
     CDS             complement(422..1690)
                     /locus_tag="VEP73_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27334.1"
                     /translation="MSDSDAEAGTPAAPTPAPAGRAGPGAAAQAAAGQPVGVLLPLRL
                     ETRFDDKRLRLRIIPDEPWFARHDPKASDGELVALERYVRAAAAGPDDPATRLAWAEL
                     VRHLGGGRAVYLDADWLALIAGAAAAHQARGCAHGLPRPGQLPGRAAAALVADRGGEG
                     RHRRYAPDRSHFATLLLIDLIVNQSDDWFTFPVDARTGHVVTLDEVVVTDSFGDDWTL
                     TPPADGWSMFATTGLGRESLVVWAVAASPLMGPVLDGVAVGMDEDANLVWAVGQRLHG
                     RDLPTPDDPPPEPPAVLDASGRQGHAYRSATRIPPHWHPYVVQEVGGRRRFVQGRAAD
                     LSGPTAALLPAAESDLLQDPASGGVHPVHQVEPAAIPTEGVRLERRAVLARRTDASPV
                     LWMQRRRLPLLTPPALRVRFDALEPVPPAV"
     gene            complement(1748..>2771)
                     /locus_tag="VEP73_12725"
     CDS             complement(1748..>2771)
                     /locus_tag="VEP73_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27335.1"
                     /translation="STAARRMLRPGPARTALSDGADPGIVMEILNRCPDPPQPSDDGL
                     PPLPDDFDERLGEIQKADVIDTRQLAERMQRLGGGLEDLPDPPTDPQDLLDLLDGLEE
                     PPDPPLVPPEVCVGLDRPVWRDLNEHFTEWLLPGVGNLPDDSVIALQTNPVFTDAFLI
                     GYNTQVVSELRWRNLRIATGCTPLRVFWERAHTGTGARVDDVIGFASWSDGSHLGAPS
                     HRPGDAAATDLVLVFRGQIFLRFPKTAFYLVSAVHGGVMDLAQNPDPSAPKRLPSFQG
                     RIGADVMFFGFQGVPATDVPRHWVALEEPRPASASGTPMATPRPRTSPTGRASPTPRS
                     ATPCAS"
BASE COUNT          348 a          987 c         1091 g          345 t
ORIGIN      
        1 gaggccaagc tcgcgatcgc ccgggccgtc gaggcgcgct tcttcgccgt gctcgccgac
       61 ggccgggttg tcagctacgc cgacctctac cgtgacgggc gcacggcgca ggtcgaggac
      121 gtcgcgacgc tcgaggcgca tcgcggccgc ggctacgcga gcgccgtcgt cctgcgcgcg
      181 gtcgaggcgg ccaggcacgc tggctgcgac ctcgtcttcc tcgtggccga cgccgacgac
      241 tggcccaagg agctctaccg ccggctcggc ttcgacgacc tcgggcgcta cgtgaagttc
      301 atccgccccg cgtgacctgg aaggtccggg gcgcgctcct gatgatcggc accgtggctt
      361 cggctggccg cccgaagccg cctgcgcgcg accaggcgac gaggctcata tcgagcggcg
      421 gtcagacggc cggcggcacg ggctcgaggg cgtcgaagcg cacgcgcagc gccggcgggg
      481 tgagcagcgg aagccggcgc cgctgcatcc acagcaccgg ggaggcgtcc gtgcggcgag
      541 cgagcaccgc ccggcgctcc aggcgcacgc cctcggtcgg gatcgccgcc ggctcgacct
      601 ggtggaccgg gtggaccccg cccgaggccg ggtcctggag caggtcgctc tcggccgccg
      661 gcagcagcgc cgcggtcggc cccgacaggt cggcggcgcg gccctggacg aaccggcggc
      721 gcccgccgac ctcctggacc acgtacgggt gccagtgcgg cgggatgcgg gtcgccgacc
      781 ggtacgcgtg cccctggcgg cccgaggcgt cgaggaccgc gggtggctcg ggcggcgggt
      841 cgtccggtgt gggcaggtcg cggccgtgga ggcgctgccc caccgcccag acgaggttgg
      901 cgtcctcgtc catgccgacg gcgacgccgt ccagcaccgg acccatcagc ggtgacgcgg
      961 cgaccgccca gaccacgagc gactcgcggc cgagcccggt ggtcgcgaac atgctccagc
     1021 cgtcggccgg gggcgtcagg gtccagtcgt cgccgaacga gtcggtgacc acgacctcgt
     1081 ccagggtgac gacgtgcccg gtacgcgcgt cgacggggaa ggtgaaccag tcgtcggact
     1141 gattgacgat gaggtcgatc aggagaaggg tcgcgaagtg gctgcggtcg ggtgcgtacc
     1201 gccgatgtcg accttcgcct cctcgatctg ccaccagcgc ggcagcggcg cgcccgggta
     1261 gctgacccgg ccggggaaga ccgtgcgcgc atccacgggc ctggtgggcg gcggcagcgc
     1321 ccgcgatcag cgccagccag tcggcgtcga ggtagaccgc ccgacccccg ccgaggtgcc
     1381 ggaccagctc ggcccaggcc agccgggtcg cggggtcgtc cggcccggcc gccgccgcgc
     1441 gcacgtagcg ctcgagcgcg accagctcac cgtcgctggc cttggggtcg tggcgtgcga
     1501 accacggctc gtcggggatg atccgcagcc gcagccgctt gtcgtcgaag cgggtctcca
     1561 gccgcagcgg cagcaggacg ccgaccggct ggcccgcggc cgcctgggcc gccgcgcctg
     1621 gccccgccct gccggccggc gcgggcgtgg gcgcggcagg cgtgcccgcc tcggcgtcgc
     1681 tgtcgctcat ctcgcggctc ccgtcgggtc ggcggtcaag ggggtggcgc caggcggtcg
     1741 ccccggatca ggacgcgcag ggcgtcgccg aacgcggcgt cggcgaagcg cgccccgtcg
     1801 gtgatgtccg aggtcggggt gtagccattg gcgttccaga agcggaagcc ggtcggggct
     1861 cctcgagcgc gacccagtgg cgcgggacgt cggtcgccgg gacgccctgg aacccgaaga
     1921 acatgacgtc ggcgccgatg cggccctgga agctcggcag ccgcttcggc gccgacgggt
     1981 ccgggttctg ggccaagtcc atcaccccgc cgtgcacggc tgagacgagg tagaacgcgg
     2041 tcttcgggaa gcgcaggaag atctgcccgc ggaacaccag caccaggtcg gtcgcggcgg
     2101 cgtcgccggg gcggtgcgac ggggcgccga ggtggctgcc gtcgctccag ctcgcgaacc
     2161 cgatgacgtc gtcgacgcgc gcgcccgtgc cggtgtgcgc ccgctcccag aagacccgca
     2221 gcggcgtgca gccggtcgcg atccgcaggt tgcgccagcg cagctcgctg acgacctggg
     2281 tgttgtagcc gatcaggaac gcgtcggtga ataccgggtt ggtctgcagc gcgatcaccg
     2341 agtcgtcggg caggttcccg acgccgggca gcagccactc ggtgaagtgc tcgttgaggt
     2401 cccgccacac cggccggtcg aggccgacgc agacctccgg cgggaccagc ggcgggtccg
     2461 gcggctcctc gaggccatcg agcaggtcca ggaggtcctg ggggtccgtc ggcgggtccg
     2521 ggaggtcctc gaggccgccg cccagccgct gcatccgctc ggcgagctgc cgggtgtcga
     2581 tgacgtcggc cttctggatc tcgccgaggc gctcgtcgaa gtcgtcgggc aacgggggca
     2641 ggccgtcgtc ggagggctgg ggcgggtccg ggcagcggtt gaggatctcc atcacgatcc
     2701 cggggtcggc gccgtcggag agggcggtgc gcgccgggcc gggacgcagc atccggcggg
     2761 ctgcggtcga g
//
LOCUS       DASPYY010000815         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2525634, whole genome shotgun sequence.
ACCESSION   DASPYY010000815 DASPYY010000000
VERSION     DASPYY010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525634"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEP73_12730"
     CDS             complement(<1..832)
                     /locus_tag="VEP73_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYT27336.1"
                     /translation="MTATRPRGMAALGLLVLLAACTHGPTAPRPPALTLPSAPGTSAG
                     RAGRAGDWTTYHRDTARTGYQAGLADPTGLAAAWSARLDGAVYAEPLVVGDRVLVTTE
                     ADSLYSLELRTGRVRWRTTVGTPVRLSELPCGNLDPLGITGTPTYDPGTGLVFAVAEV
                     TGPAHVLVGVDARTGRVRVRRPADPPGIDPRPHQQRAALALSRGRVYVAYGGLLGDCG
                     DYHGRVVASRTDGRGPLQAFQVPTGREGGIWAASGPAVDAGGRLYVAVGNGAATGGAW
                     D"
     gene            complement(1396..1923)
                     /locus_tag="VEP73_12735"
     CDS             complement(1396..1923)
                     /locus_tag="VEP73_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HYT27337.1"
                     /translation="MRLEGKRVIALVEDEYEDLELWYPVLRLREEGAEVVVAGLGAPS
                     YTGKYGLVAEPDAHVDELDMADFDGILVVGGWAPDKLRRSEKVQQLVRDADAAGKLLG
                     VICHGGWVPASAGVLKGRTMTCTVGIRDDVMNAGATYVDEPVVVDGNLVTARRPPDLP
                     EYGAALVEALSKGTA"
     gene            2071..3153
                     /locus_tag="VEP73_12740"
     CDS             2071..3153
                     /locus_tag="VEP73_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYT27338.1"
                     /translation="MATDEERIALFIDHENLVIGAREIGRAFDVGPIFDALAERGRVV
                     ARRAYADWTLFADDRRSLVQHNCELIDIPQRTGAVRKNAADIKLAVDAMELAYERGFV
                     STFVIASGDSDFTPLVAALRGLNRRVIGIGVKGSTSGLLPPACDEFMFYDRLPGVGEL
                     NQQPKRPGNGNGPAKTKPPARAKAGKAKARAPGTQDDRDLQDLQRVITQTLAGLKQSG
                     SEPVLASTLKRAVLRKDPTFSESDYGFRAFGELLRQLERNGVVALTDGAASGDPAIDF
                     PAAGGPSDQAFDLLKKTLLDLQGEDDDVPLSGLKDQLRKRAPGWSEKDLGYAGFLQFA
                     KAAASSGIVAMEWDDEEGDYFLYVPD"
     gene            complement(3138..3665)
                     /locus_tag="VEP73_12745"
     CDS             complement(3138..3665)
                     /locus_tag="VEP73_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT27339.1"
                     /translation="MPVPPPPAPAPSVRVRPARAEELAGVGRLTVAAYAADVSPSSSY
                     AAVLADAAGRAAHARLLVAVDSANGGLLGTATLVLGGPYAQLAGPGEAELRMLAVDPA
                     ARRRGVGEALVRATVAEARAAACRRYVLSTLPTMVAAHRLYERLGFRRAPALDHDPVA
                     GVHLLAYALDLQSGT"
     gene            complement(3766..4410)
                     /locus_tag="VEP73_12750"
     CDS             complement(3766..4410)
                     /locus_tag="VEP73_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HYT27340.1"
                     /translation="MPFTYPVLLEIEGRRCVVVGGGPETVQKTTPLVEQGALVTVVTP
                     AAEPGLEELAAAHPDRVAVRHRAWREGDLAGAFLCIAATGDPELNAKIHAEGAREGVL
                     VNAVDDPAHCDFAHPALVRRGDLVVAVGTGGKAPALAKRIRRELADRYGGEYGEAAAL
                     IGEVRAETRASWPSFAAWAERWQAALDDQVIGLVREGRADDARALLLARLAGPA"
     gene            complement(4428..5279)
                     /locus_tag="VEP73_12755"
     CDS             complement(4428..5279)
                     /locus_tag="VEP73_12755"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYT27341.1"
                     /translation="MTEPDAAAEARAAADRLAGASAADVLAWATATFGDRFAVVTAFQ
                     AEGMVVLDLARRADPAVRVLTIDTGRLPQETYDVIETVRARWGMTVEVLAPDTRAVEA
                     MTSRHGPNLFRRDQALRLLCCQVRKVDPLAGALAGLDAWASGLRRDETPARARVAPVE
                     VDLTHGGIVKLNPLATWSREQVWAYVREHGLPVHPLYERGYTSIGCAPCTRAVAPGEG
                     RTEGRWWWEAGADRECGLHHQTPSERFDAALTTLRADVGGTPRRAERDRDVGGTPPAG
                     HAGAAGR"
     gene            complement(5276..>6149)
                     /gene="sat"
                     /locus_tag="VEP73_12760"
     CDS             complement(5276..>6149)
                     /gene="sat"
                     /locus_tag="VEP73_12760"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776549.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HYT27342.1"
                     /translation="GAAGQHRRAAGGAAGDQAWRPDRRAEAAAVLGTTDPAHPGVAAT
                     LARPGPLVGGAVHVLRLPASPPGLGPRLTPAEVRAEAAARGWRTMVGFQTRNPVHRAH
                     EHLTKVALEQVDGLLLHPLVGETKGDDVPAALRMTCYRALLDGYYPPSRVLLSAFPAA
                     MRYAGPREAVCHALVRKNYGCSHFIVGRDHAGVGSYYGTYAAQEAFGAYKPDELGVVP
                     LKFEHAFWCRACGGMATARTCPHPAGARVSLSGTAVRAMLGRGELPPPEFTRPEVARL
                     LADGYRDTRREGST"
BASE COUNT          789 a         2343 c         2289 g          728 t
ORIGIN      
        1 ggtcccacgc cccgccggtc gccgcgccgt tgccgacggc gacgtacagg cggccgccgg
       61 cgtcgaccgc cggtcccgag gcagcccaga tgccgccctc ccggccggtc gggacctgga
      121 aggcctgcag tggcccgcgg ccgtcggtgc gcgaggcgac cacccggccg tggtagtcgc
      181 cgcagtcgcc gagcaggccg ccgtaggcga cgtagaccct gccccgggag agggccaggg
      241 cggcgcgctg ctggtgcggc cgggggtcga tgccgggcgg gtcggccggg cggcggaccc
      301 gcacccggcc ggtgcgggcg tccaccccga ccaggacatg ggccgggccg gtcacctcgg
      361 cgaccgcgaa gaccaggccg gtgcccgggt cgtaggtggg cgtgccggtg atgccgagcg
      421 ggtcgaggtt gccgcagggc agctcggaca ggcgcaccgg cgtgccgacc gtggtgcgcc
      481 agcgcacccg tccggtgcga agctcgagcg agtacagcga gtcggcctcg gtggtcacca
      541 gcacccggtc gccgaccacc agcggctcgg cgtacaccgc tccgtccagg cgggcgctcc
      601 aggccgccgc caggccggtc gggtcggcga ggccggcctg gtagccggta cgggcggtgt
      661 cgcggtggta ggtggtccag tcgccggctc gcccggcccg gccagccgac gtgcccgggg
      721 ccgacggcag cgtcagcgcc gggggccggg gcgccgtcgg cccgtgggtg caggcggcga
      781 gcaggacgag caggccgagc gcggccatcc cgcgaggtcg cgttgcggtc atggccacct
      841 ccgctgccgc ggcttgccgg tgcgatgccg ccccaccgcc gcctagcacc gggatcgcga
      901 tgcacctccg gcagcggggg cacgacgtcc cacggcgtgg ccgtccaaac cgtctacttc
      961 gccttccgca ccaaggcgga actgctgatc gccgcctacg accaggccgt cctgggcagc
     1021 ctggacgccc cccaccccga ccagcaggac ccgcacctgc gcgtcctcgc ccaggccgag
     1081 cacgacgcca cccaggcgct cgggcacttc gtcgacgggt gtatgggcat ccttggccgc
     1141 tccgccccac tccaaccggt catggtcacc agccccgacg acgaggtccg ccgtgctctc
     1201 cccggagctg cacggcatcc tgtgcacctc ccgcgggtgg gcacccgagg acttcaagca
     1261 atggggtgct caccaccctc cgaacccagc tcctccatcc cagggacctc agcggcatcg
     1321 cagaactcaa tcgccggcgg ggagcaccaa gcacatgacc agcgaagccg tggtcggggt
     1381 gtgcggctcc gccggctacg ccgtgccctt gctcagcgcc tcgaccagcg cggcgccgta
     1441 ctcgggcagg tccggcgggc gccgggcggt gaccaggttg ccgtcgacca ccaccggctc
     1501 atcgacatac gtggccccgg cattcatcac gtcatctcga atcccaaccg tgcaggtcat
     1561 ggtccggccc ttgagcaccc ccgccgacgc cggcacccag ccgccatggc agatgacgcc
     1621 gagcagctta cctgcggcgt ccgcgtctcg gaccagctgc tgcaccttct cggagcggcg
     1681 cagcttgtca ggtgcccagc cgccgacgac caggatgccg tcgaagtcgg ccatgtcgag
     1741 ctcgtccacg tgcgcgtccg gctccgccac caagccgtac ttgcccgtgt agctgggcgc
     1801 gccgaggccg gcgacgacca cctcggcgcc ttcctcgcgc aggcgcagca ccgggtacca
     1861 gagctccagg tcctcgtact cgtcctcgac cagcgcgatc acccgcttgc cttcgagtcg
     1921 catgccgcct ccctcgaggt aggtcgccgg ccggcgggat cgcgtccgcc gacgtggccc
     1981 agcgtacgcc ctgacgggcc gttctccggc gcgccccgcg gcctgtggac ggggttcgga
     2041 accgcgggcg aaacggcgta tggtttgccc atggccaccg acgaagaacg catcgcactg
     2101 ttcatcgacc acgagaacct cgtcatcggc gcccgcgaga tcggccgcgc cttcgacgtc
     2161 ggtccgatct tcgacgcgct tgccgaacgg ggccgggtcg tggccaggcg tgcctacgcc
     2221 gactggacgc tgttcgccga cgaccgtcgc tccctcgtcc agcacaactg cgagctgatc
     2281 gacatcccgc agcgcaccgg tgcggtccgc aagaacgcgg ccgacatcaa gctcgcggtc
     2341 gacgcgatgg agctcgccta cgagcgcggc ttcgtgtcga cgttcgtgat cgcctccggc
     2401 gactcggact tcacgccgct cgtggcggcg ctgcgagggc tcaaccggcg ggtgatcggg
     2461 atcggggtca agggctcgac gtccggcctg ctcccccccg cctgcgacga gttcatgttc
     2521 tacgaccgcc tgcccggcgt cggcgagctg aaccagcagc cgaagcggcc cggcaacggc
     2581 aacgggccgg ccaagaccaa gccgcccgcc cgcgccaagg ccggcaaggc caaggcccgc
     2641 gcgcccggca cccaggacga ccgtgacctg caggacctgc agcgcgtcat cacccagacg
     2701 ctcgccgggc tgaagcagag cggctcggag ccggtgctcg cctcgaccct caagcgggcg
     2761 gtcctgcgca aggacccgac cttctcggag tccgactacg gcttccgcgc gttcggggag
     2821 ctcctgcgcc agctcgagcg caacggggtc gtcgccctca ccgacggtgc cgcctcgggc
     2881 gaccccgcga tcgacttccc cgcggccggc ggcccctccg accaggcgtt cgacctgctc
     2941 aagaagacgt tgctcgacct gcagggcgag gacgacgacg tgccgctgtc cggcctcaag
     3001 gaccagctgc gcaagcgcgc gccgggctgg tcggagaagg acctcgggta cgccggcttc
     3061 ctgcagttcg ccaaggcggc cgcgtcgagc gggatcgtcg ccatggagtg ggacgacgag
     3121 gaaggcgact acttcctcta cgtgcccgac tgaaggtcga gcgcgtaggc gagcaggtgc
     3181 accccggcca ccgggtcatg gtcgagcgct ggggcgcgcc ggaacccgag ccgctcgtag
     3241 agccggtgcg cggccaccat ggtcggcagg gtggacagca catagcgccg gcaggcggct
     3301 gcgcgtgcct ccgcgacggt ggcgcgcacg agcgcctccc cgaccccgcg ccgacgggcg
     3361 gccgggtcga ccgcgagcat ccgcagctcg gcctcgcccg ggccggcgag ctgggcgtag
     3421 gggccgccga gcacgagggt cgcggtaccg aggagcccgc cgttggcgga gtcgaccgcg
     3481 accagcagcc gggcgtgcgc ggcccgcccg gccgcgtcgg ccagcaccgc tgcatacgag
     3541 gagctcgggc tgacgtcggc ggcgtaggcc gcgacggtga gccggccgac gccggcgagc
     3601 tcttcggcgc gggcgggacg gacccggacc gacggcgccg gcgcgggagg cggtgggacg
     3661 ggcacgcggt agtaggtacc acacctcgcg gccgccgggc atccggtccg ggcaggcgga
     3721 gccaggccgc ccgcctcacg gccgacctcg gcgcgccgac ggcggtcagg ccggcccggc
     3781 cagccgcgcg agcagcagcg cgcgggcgtc gtccgccctc ccctcgcgca ccagcccgat
     3841 gacctggtcg tccaaggccg cctgccagcg ctcggcccag gcggcgaagc tcggccagct
     3901 cgcgcgcgtc tcggcgcgga cctcgccgat cagggcggcc gcctcgccgt actcgccgcc
     3961 gtagcggtcg gccagctcgc ggcggatgcg cttggccagg gcgggcgcct tcccgccggt
     4021 cccgacggcg acgaccaggt cgccgcggcg taccagggcc gggtgggcga agtcgcagtg
     4081 cgcggggtcg tcgaccgcgt tcaccagcac gccctcgcgt gcgccctcgg cgtggatctt
     4141 ggcgttcagc tcggggtcgc cggtcgcggc gatgcacagg aacgcaccgg ccaggtcccc
     4201 ctcgcgccag gcgcggtggc gcacggcgac ccggtccggg tgggccgcgg cgagctcctc
     4261 caggcccggc tcggcggcgg gcgtgaccac cgtgacgagc gcgccctgct ccaccagcgg
     4321 cgtcgtcttc tggacggtct cgggaccgcc gccgaccacg acgcagcgcc gcccctcgat
     4381 ctcgaggagc accgggtagg tgaacggcac gccggccccc tcccccgtca gcgccccgcc
     4441 gcgccggcgt gcccggcggg cggcgtgccg ccgacatccc tgtcccgttc ggcacgccga
     4501 ggcgtgcctc cgacgtccgc gcgcagcgtg gtgagcgcgg cgtcgaaccg ctcggacggg
     4561 gtctggtggt gcaggccgca ctcgcggtcg gcgcccgcct cccaccacca gcggccctcc
     4621 gtcctgccct cgccgggcgc gaccgctcgg gtgcacggcg cgcagccgat cgaggtgtag
     4681 ccgcgctcgt agagagggtg gacgggaagc ccgtgctcgc ggacgtaggc ccagacctgc
     4741 tcgcgcgacc aggttgcgag cgggttgagc ttgacgatgc ccccgtgggt gaggtccacc
     4801 tcaacggggg cgacccgcgc gcgggctggg gtctcgtcgc gccgcagccc cgacgcccac
     4861 gcgtccaggc cggcgagcgc gccggcaagc gggtcgacct tgcggacctg gcagcagagc
     4921 aggcgcaggg cctggtcgcg ccggaacagg ttcgggccgt gccgcgaggt catcgcctcg
     4981 accgcccggg tgtcgggcgc gagcacctcg acggtcatcc cccacctggc gcgcacggtc
     5041 tcgatgacgt cgtaggtctc ctgcgggagc cggccggtgt cgatcgtcag cacccgcacg
     5101 gccgggtcgg cgcggcgggc gaggtcgagc accaccatcc cctcggcctg gaacgcggtg
     5161 acgaccgcga accggtcgcc gaacgtggcg gtggcccagg cgagcacgtc ggcggctgac
     5221 gcgccggcca ggcggtcggc cgcggcgcgg gcctccgccg ccgcgtcggg ctcggtcacg
     5281 tcgacccctc ccgacgcgtg tcgcggtagc cgtcggcgag caggcgcgcc acctcggggc
     5341 gggtgaactc gggtgggggg agctcgccgc ggccgagcat cgcccgcacg gccgtgcccg
     5401 acagcgagac gcgggcgccg gccgggtgcg ggcaggtgcg ggcggtcgcc atgccaccgc
     5461 acgcgcggca ccagaaggcg tgctcgaact tgagcgggac gacgccgagc tcgtccggct
     5521 tgtaggcgcc gaaggcctcc tgcgccgcgt aggtgccgta gtagctgccc acaccggcgt
     5581 ggtcgcgccc gacgatgaag tggctgcacc cgtagttctt gcggacgagc gcatggcaca
     5641 ccgcctcgcg cggcccggcg tagcgcatgg cagccgggaa ggccgacagc agcacccggc
     5701 tgggcgggta gtagccgtcc agcaaggccc ggtagcaggt catgcggagc gccgccggca
     5761 cgtcgtcgcc cttggtctcg ccgaccagcg ggtgcagcag cagcccgtcg acctgctcga
     5821 gggcgacctt ggtgaggtgc tcgtgggccc ggtggaccgg gttgcgggtc tggaacccga
     5881 ccatcgtccg ccagccgcgg gcggccgcct cggcgcggac ctcggccggg gtgagccgcg
     5941 ggccgagccc gggcggggac gcgggcaggc gcaggacgtg gacggcgccg ccgacgagcg
     6001 ggccggggcg cgcgagggtg gcggcgacgc cggggtgggc cgggtcggtg gtgcccagga
     6061 ccgcggccgc ctccgcgcgg cggtccggcc gccaagcctg gtcacccgca gcgccgccag
     6121 cagcccgccg gtgctgtccc gcagcgcca
//
LOCUS       DASPYY010000816         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2244498, whole genome shotgun sequence.
ACCESSION   DASPYY010000816 DASPYY010000000
VERSION     DASPYY010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244498"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VEP73_12765"
     CDS             complement(<1..214)
                     /locus_tag="VEP73_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYT27343.1"
                     /translation="MPGAASARRWRARAGALLFGAPLALAFPRPSLWWLAFVALVPLL
                     ALVRAAPAAREAAILAGLGGAGFFLAV"
     gene            complement(250..1716)
                     /locus_tag="VEP73_12770"
     CDS             complement(250..1716)
                     /locus_tag="VEP73_12770"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYT27344.1"
                     /translation="MSIEASERKVVEQVPPKLYIGGTWRDAASGATFKVEDPSTGEPL
                     CEVADASREDGLAALGAAAAVQQEWAAHPPRERGEILRRAFEAITARSDELALLMTLE
                     MGKPVAESKAEIAYAAEFFRWFAEEAVRIDGQYAVTPAGNGRMLTMRQPVGPCLLITP
                     WNFPMAMGTRKIGPAVAAGCTMVMKPAQLTPLSMLALTQILEQAGLPGGVLNLVTTSS
                     SGRVMEPLIKDPRTRKLSFTGSTEVGRRLMAQAADNVLRVSMELGGNAPFLVFEDADL
                     DAAVEGAMIAKMRNIGEACTAANRFHVAAPVADAFASKLAERMAALKVGRGTEEGVQV
                     GPLIDGAAVEKVTELVGDAVGKGAKAVVGGRARDGRGYFYEPTVLTDVPGDARLLKEE
                     IFGPVAPVASFDSEEAAVAAANDTEFGLVAYVYTSDLKRAFRVVEGLETGMVGLNQGM
                     VSNPAAPFGGVKQSGIGREGGKEGIHEYLEIKYVAMNL"
     gene            1839..2744
                     /locus_tag="VEP73_12775"
     CDS             1839..2744
                     /locus_tag="VEP73_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYT27345.1"
                     /translation="MIGACRRGGGPAIVDAVAAGWTAAARRLALALAAAVLLGGCTGG
                     RDARTSPAAPEAPGKAAWRRLAPAPSERTEVAATAVGTLVYVVGGFRDDGTTVPTVEV
                     YDTRQDRWRRGPDLPEARAAGTAVALDGRLYVAGGVAPGGLAGAMLVYDPTARRWSTA
                     PGPPTRREHLGGAGYGGRVYTVGGRARGRGDFTAAEAFNPGTGQWVRLPDLPTRRGGL
                     AAAATGNGFVVAVGGEATTTFEEAETFDARVGAWSSLPPLPTPRHGLGVVAVGTVLYA
                     LAGGPRPGLHVSGAAEAIDLAPLRP"
     gene            2877..>3133
                     /locus_tag="VEP73_12780"
     CDS             2877..>3133
                     /locus_tag="VEP73_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT27346.1"
                     /translation="MTTANRDPLDVLVVGAGPTGLTLAAQLRAFGATCRIVDRQPDRV
                     HESRALAVQPRTLEVLRGLGVTEELVARGNDALWVQLHAGGR"
BASE COUNT          368 a         1172 c         1192 g          401 t
ORIGIN      
        1 cgaccgcgag gaagaacccg gcccccccga gcccggcgag gatggccgcc tcgcgcgcgg
       61 ccggggcggc gcggacgagc gcgaggagcg ggaccagggc gacgaacgcg agccaccaca
      121 gggaggggcg cgggaaggcg agggcgaggg gcgcgccgaa caggagcgcc cccgcccgcg
      181 cgcgccaccg gcgcgcgctc gccgccccgg gcacggtgcc ctgccagcca gccaggcccc
      241 gccgggcggc tagaggttca tggcgacgta cttgatctcg aggtactcgt ggatcccttc
      301 cttgccgccc tcgcggccga tcccggactg cttcacgccg ccgaacgggg cggccgggtt
      361 cgacaccatg ccctggttga ggccgaccat cccggtctcg agcccctcga cgacccggaa
      421 cgcgcgcttc aggtcgctcg tgtagacgta ggcgaccagg ccgaactcgg tgtcgttggc
      481 ggcggcgacg gccgcctcct cggagtcgaa ggaggcgacg ggcgcgaccg gcccgaagat
      541 ctcctccttg agcaggcgcg cgtcgccggg gacgtcggtc agcaccgtgg gctcgtagaa
      601 gtagccgcgg ccgtcgcggg cgcggccccc gaccacggcc ttcgcgccct tgccgaccgc
      661 gtcgccgacg agctcggtga ccttctcgac cgcggcgccg tcgatgagcg gcccgacctg
      721 gacgccctcc tcggtgcccc ggccgacctt gagggcggcc atgcgctcgg ccagcttgga
      781 cgcgaacgcg tcggcgaccg gcgcggcgac gtggaagcgg ttggccgcgg tgcatgcctc
      841 gccgatgttg cgcatcttcg cgatcatcgc gccctcgacc gcggcgtcga ggtcggcgtc
      901 ctcgaagacg aggaacggcg cgttgccgcc gagctccatc gacacccgca ggacgttgtc
      961 ggcggcctgg gccatgagcc tgcggccgac ctcggtggag ccggtgaacg acagcttgcg
     1021 cgtccgcggg tccttgatga gcggctccat cacccggccc gacgaggagg tggtgacgag
     1081 gttgagcacg ccgccaggga ggccggcctg ctcgaggatc tgggtcaggg cgagcatcga
     1141 cagcggggtc agctgcgccg gcttcatgac catcgtgcag ccggcggcca ccgccgggcc
     1201 gatcttgcgg gtgcccatgg ccatcgggaa gttccacggc gtgatgagca ggcagggccc
     1261 gaccggctgg cgcatggtga gcatgcggcc attgccggcc ggggtgaccg cgtactggcc
     1321 gtcgatgcgg accgcctcct cggcgaacca gcggaagaac tcggccgcgt acgcgatctc
     1381 cgccttcgac tcggcgaccg gcttgcccat ctccagggtc atcagcaggg ccagctcgtc
     1441 gctgcgggcc gtgatcgcct cgaacgcgcg gcgcaggatc tcgccccgct cgcgcggcgg
     1501 gtgcgcggcc cactcctgct ggacggcggc cgcggccccg agcgcggcca ggccgtcttc
     1561 gcggctcgcg tcggccacct cgcacaacgg ctcgccggtc gacgggtcct cgaccttgaa
     1621 tgtcgcgccg ctcgccgcgt cccgccacgt cccgccgatg tagagcttgg gggggacctg
     1681 ctcgacgacc ttccgttcgc tggcttcgat gctcatggcg cctggtctcc ttccgggccg
     1741 gtcgcgctgg ccgtcgcctc gtcctggcaa tgaagatagc gccgcgccga cccggcgcgc
     1801 cggtcgctgc ccggttgccg ggcggcgccc gatgcggtgt gatcggtgcc tgtcgccggg
     1861 gaggaggtcc cgccatcgtc gacgccgtcg ccgccggctg gaccgccgcg gcccgccggc
     1921 tggcgctcgc gctcgcggcg gccgtgctgc tcggcggctg caccgggggc cgcgacgcgc
     1981 ggaccagccc ggcggccccg gaggcacccg ggaaggccgc gtggcggcgc ctcgcgccgg
     2041 cgccgtcgga gcggaccgag gtcgccgcga ccgccgtcgg gacgctggtg tacgtggtcg
     2101 gcggcttccg ggacgacggg acgaccgtgc cgaccgtcga ggtgtacgac acgcgccagg
     2161 accgctggcg gcgcggcccg gacctgcccg aggcgcgggc ggctggcacc gccgtcgccc
     2221 tggacggccg cctctacgtg gccggcgggg tcgcaccggg cgggctcgcc ggcgcgatgc
     2281 tcgtctacga cccgaccgcc cggcgctggt cgaccgcgcc cggcccgccg acgcggcggg
     2341 agcacctggg cggcgccggc tacggcgggc gcgtgtacac ggtcgggggg cgggcccggg
     2401 gacgggggga cttcaccgcg gccgaggcct tcaacccggg gacggggcag tgggtcaggc
     2461 ttcccgacct gcccacccgc cgcggcgggc tggccgcggc cgcgaccggc aacgggttcg
     2521 tggtggcggt gggcggcgag gcgacgacca ccttcgagga ggcggagacg ttcgacgcgc
     2581 gcgtgggcgc ctggtcgtcg ctgccgcccc tgccgacccc gcgccacggc ctcggcgtcg
     2641 tcgcggtcgg gacggtcctc tacgcgctcg cgggcggccc tcggccgggt ctgcacgtct
     2701 ccggtgccgc cgaggcgatc gacctcgcgc cgctgcggcc ctgaccgctg ctccgtgagc
     2761 agcaccaggc ctcctcgccg caccggcgca accgccgctt gcccgccgtg gcggtagcgg
     2821 aacgagtaga ggtgctatag catagcatca ctatctatga tgcgttgcta cagatcatga
     2881 cgaccgccaa ccgcgacccg cttgacgtgc tggtcgtcgg cgccgggccg accggcctga
     2941 ccctggccgc ccagctgcgg gcgttcgggg cgacctgtcg gatcgtcgac cggcagcccg
     3001 accgcgtcca cgagtcgcgg gcgctggctg tccaaccacg caccctggag gtcctgcgcg
     3061 gcctcggggt gaccgaggag ctggtcgcgc gcggcaacga cgcgctctgg gtccagctcc
     3121 acgctggcgg ccg
//
LOCUS       DASPYY010000817         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1683262, whole genome shotgun sequence.
ACCESSION   DASPYY010000817 DASPYY010000000
VERSION     DASPYY010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683262"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VEP73_12785"
     CDS             complement(<1..389)
                     /locus_tag="VEP73_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT27347.1"
                     /translation="MFKRKRDGAPAATASVDGDGRPALLVAEHVRKVYRTGEIEVEAL
                     ADLDLTVHQGEMVAVMGPSGSGKTTLLNCLSGLDDIDAGRVLVAGQDLFAMSDAVRTT
                     HRATSMGFIFQAFNLIPVFTAAENVELP"
     gene            complement(1115..1870)
                     /locus_tag="VEP73_12790"
     CDS             complement(1115..1870)
                     /locus_tag="VEP73_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HYT27348.1"
                     /translation="MEYWTPASALVIAAHPDDIEFGCAGTVARWVSEGTEVTYALLTN
                     GAAGSSDPEMTRERLAELREAEQLAAAKVVGVGRVEFLGHEDGLLEPTLEVRKQVVRL
                     IRQVRPEVVVTTDPTTRYFADRYVNHPDHRAAGEATLAAVIPGSDTRLAYPELLAEGL
                     EPVKLTGVLLLMSMEPNLVVDIGDHLATKLESLRCHASQLGEQSWEELAAGLAGMAEF
                     SAAGQPFRYGEAFRALRFDQPWRPEPEGQLSEA"
     gene            2018..>2255
                     /locus_tag="VEP73_12795"
     CDS             2018..>2255
                     /locus_tag="VEP73_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HYT27349.1"
                     /translation="MTELEMAVPVTGSTVWWSTLDREGRERLGRRGELDPTPDVLVVG
                     GGAVGLATAALCRRAGLGRVQVVERAAVIASAASG"
BASE COUNT          279 a          820 c          832 g          324 t
ORIGIN      
        1 ggcagctcga cgttctcggc cgcggtgaac accgggatca ggttgaacgc ctggaagatg
       61 aagcccatcg aggtcgcccg gtgggtcgtg cgcaccgcgt ccgacatcgc gaacaggtcc
      121 tgcccggcca ccaggacccg gcccgcgtcg atgtcgtcga ggccggacag gcagttgagc
      181 agggtcgtct tccccgatcc ggacggcccc atcacggcga ccatctcacc ctggtgcacg
      241 gtgaggtcga ggtcggccag tgcctccacc tcgatctcgc cggtgcggta caccttgcgg
      301 acgtgctccg ccacgagcag cgcgggccgg ccgtcgccgt ccacgctcgc cgtcgcggcc
      361 ggcgcaccgt cccgcttccg cttgaacatc gttcttcacc tttcctcgtc cacaacgaag
      421 tcgcccggta tgttcacggg ccccctggcg ccgggcggcg tggccggcca ccgcccgggg
      481 cggatgcggc gcccgcggcg caggcaccgc actgcgcggg gcgccagagg ccatccccgc
      541 ggcagcccgc tcccccctgc ccatcatcgg cgacggggag cctgtgcgcg agcaaattta
      601 ccagttcgag catctatgct cgaccgcgcc ctgaattgtt ccatgtccgt ccacgcgccg
      661 ttcacgtgcg gccccctcgc gccgcgtttc taatgacatt gcgacccgcg gcattgcgat
      721 ccgcgagccc cgcaagcgtc gccggtccgc gcggcgggcg ccatcgggca tcgcccgggt
      781 cggtccctga acggccgtcg gggtgctcag ggtcatcacc cacccgcgcg ccgagcatgg
      841 ccggtgcctg ctgtggcgtt cctgagcccg cctgaggacc ctctcaggaa acccggccag
      901 ccgcgggtcg agcccgagca cgctcccacg gggcccctcc aggtcgccac cgtcatgatg
      961 ccactgcctc cctgccggtc gatcgcggtt gtggtgagga gggtgcgccg cggggctgcc
     1021 acggccctgc cggcgggctg acaccgctga cacggcgctg acgtgccggc gggaccagga
     1081 gccgtggccg cgtgacggcg cgggcatgcc cggttcacgc ctcgctgagc tggccctccg
     1141 gctcgggccg ccagggctgg tcgaagcgga gggcccggaa cgcctccccg tagcggaacg
     1201 gctgcccggc ggcggagaac tcggccatgc cggccaggcc cgccgcgagc tcctcccacg
     1261 actgctcgcc gagctggctg gcgtggcagc gcaggctctc cagcttggtg gccaggtggt
     1321 cgccgatgtc gacgaccagg ttgggctcca tgctcatcag cagcagcacc ccggtcagct
     1381 tgaccggctc caggccctcg gcgagcagct ccgggtaggc gagcctggtg tcggagccgg
     1441 ggatgaccgc ggccagggtc gcctccccgg ccgcccggtg gtcggggtgg ttgacgtagc
     1501 ggtcggcgaa gtagcgggtg gtcgggtcgg tggtgacgac cacctcgggc ctgacctggc
     1561 ggatcagccg cacgacctgc ttgcggacct ccagggtcgg ctcgagcagc ccgtcctcgt
     1621 ggccgaggaa ctccacccgg ccgaccccca cgaccttggc ggcggccagc tgctccgcct
     1681 cgcgcagctc ggccaggcgc tcgcgggtca tctcggggtc gctggacccg gcggccccgt
     1741 tggtgagcag ggcgtaggtg acctcggtgc cctcgctgac ccagcgggcg accgtgccgg
     1801 cgcagccgaa ctcgatgtcg tcgggatgcg ccgcgatgac gagcgcgctg gcaggggtcc
     1861 agtactccat ggttcacgtg tccttcgaga agaggaagcc ggtcgcaggg tgcaacgccc
     1921 cgcggccccg cctgcttccg ggcggtagga ggatcgatac catgcgggtg cgaccgtgcg
     1981 ggacggcggg tgggaccgtg cgggacggag gtcgccgttg acggagctgg agatggccgt
     2041 gccggtcacg ggctcgacgg tctggtggag cacgctcgac cgcgagggcc gggagcggct
     2101 cggccggcgc ggggagctcg acccgacccc cgacgtcctg gtggtcggag gcggcgcggt
     2161 ggggctcgcc accgcggcgt tgtgccggcg cgccgggctg ggccgggtgc aggtggtcga
     2221 acgagccgcc gtcatcgcgt cggcggcctc gggtg
//
LOCUS       DASPYY010000818         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1683337, whole genome shotgun sequence.
ACCESSION   DASPYY010000818 DASPYY010000000
VERSION     DASPYY010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683337"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1412)
                     /locus_tag="VEP73_12800"
     CDS             complement(153..1412)
                     /locus_tag="VEP73_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT27350.1"
                     /translation="MRVAMVSEHASPLAVLGGVDAGGQNVHVAALSAAMARRGAEVVV
                     YTRRDDPGLPRRVAFQPGVTVEQVDAGPAEPVGKDELLPHMDEFADRLWWSWRADPPD
                     VVHAHFWMSGRAALAAGRRLGLPVVQTFHALGVVKRRHQGARDTSPPQRTLEEATIVR
                     RADAIVATCSDEVFELARMGADLRRISVVPCGVDLDQFTPAGPAEARAPGRHRLVVVS
                     RLVERKGIGNAVTALARVPGAELVVAGGPDAELLGADPEARRLQALAARAGVAGRVRF
                     RGRVGRGAMPALLRSADVVVCVPWYEPFGIVPLEAMACGAPVVASAVGGLVDTVVDGV
                     TGLHVPPRRPDLLAEALHALLADPARRVALGRAGARRARRRYGWDRIAGATLDVYGEL
                     VGARAGRTARRGPGRSRRDAREERVWR"
     gene            complement(1409..>2112)
                     /locus_tag="VEP73_12805"
     CDS             complement(1409..>2112)
                     /locus_tag="VEP73_12805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT27351.1"
                     /translation="PGRDVPAVYLEHNAPQGRVAELRHPAADRPDLTVVHVTHFNDLF
                     WDCGGTPTRVVEHGVVDPGERYTGELPRAAAVVNEARRRGRVTGTDLLGRLGAAVPVD
                     LFGMGAREVGPPVHPVDDLPQARLHDEMARRRLYLHPVRWTSLGLSLLEAMHLGMPVV
                     ALATTEVVEAVPPDAGVISTSVDVLAAAARAFAADPERARLAGKSARATALARYGLDR
                     FLADWDRLLEEVRSA"
BASE COUNT          255 a          831 c          815 g          211 t
ORIGIN      
        1 gtcgaggccg gccaggaccg cggcgagcgc ggcgccgccc ggccggcagc gctcctcggg
       61 ctcgtcgacg accgggacgg gcgcgtccgg gctgagccgc tcgacccggc cgtcgaggtc
      121 gcggtcgagc agggcgtccc ccagcacgac gatcatcgcc acacccgctc ctcccgcgcg
      181 tccctgcggg agcggccagg cccccttcga gcggtccggc cggcgcgtgc cccgaccagc
      241 tcgccgtaca cgtccagggt ggcccccgcg atccggtccc agccgtagcg gcggcgggcg
      301 cggcgcgcgc ccgcccggcc gagcgcgacg cggcgcgccg ggtcggcgag cagggcgtgg
      361 agcgcctcgg cgaggaggtc gggacggcgc ggcggcacgt gcaggccggt gaccccgtcg
      421 accacggtgt cgaccaggcc gcccacggcc gacgcgacca ccggcgcacc gcaggccatc
      481 gcctcgagcg ggacgatccc gaacggctca taccagggca cgcagacgac cacgtcggcc
      541 gagcggagca gcgccggcat ggcaccgcgg ccgacccggc cgcggaaccg gacccgcccg
      601 gccacgccgg ccctggccgc gagcgcctgg aggcggcgcg cctccgggtc ggcgccgagc
      661 agctcggcgt ccggcccgcc cgcgaccacg agctcggcgc ctggcaccct ggccagggcc
      721 gtgaccgcgt tcccgatgcc cttgcgctcc acgagccggc tcacgaccac gaggcggtgc
      781 cggcccggcg cccgggcctc ggccggcccg gccggggtga actggtccag gtccaccccg
      841 cagggcacga cggagatgcg gcgcaggtcg gcgcccatcc gggcgagctc gaagacctcg
      901 tccgagcagg tcgccacgat cgcgtccgcc cgccgcacga tcgtggcctc ctcgagggtg
      961 cgctggggcg ggctcgtgtc cctcgccccc tggtggcggc gcttgaccac gccgagggca
     1021 tggaacgtct gcaccacggg aaggccgagc cggcggccgg cggccagcgc cgcccggcca
     1081 gacatccaga agtgggcatg gaccacgtcc ggcgggtcgg cccgccagga ccaccacagc
     1141 cggtcggcga actcgtccat gtggggcagc agctcgtcct tgcccacggg ctcggcgggg
     1201 ccggcgtcga cctgctcgac ggtcacccct ggctggaagg cgacccgccg gggcaggccg
     1261 gggtcgtccc gcctggtgta gacgacgacc tcggcgccgc gccgcgccat cgccgccgac
     1321 agcgcggcca cgtgcacgtt ctgccctccg gcgtcgacgc cgccgagcac ggccaggggg
     1381 ctcgcgtgct cgctcaccat cgcgaccctc atgcgctgcg cacctcctcg agcaggcggt
     1441 cccagtcggc gaggaagcgg tcgaggccgt agcgggcgag cgcggtggcg cgggccgact
     1501 tgccggccag gcgcgcccgc tccgggtcgg cggcgaacgc gcgcgccgcg gcggcgagca
     1561 cgtcgacgct ggtcgagatc acgcccgcat cgggcggcac ggcctcgacg acctcggtcg
     1621 tggccagcgc gaccaccggc atcccgaggt gcatcgcctc gagcagcgac agcccgagcg
     1681 aggtccagcg caccgggtgc aggtagagcc ggcgccgcgc catctcgtcg tgcaggcggg
     1741 cctggggcag gtcgtcgacc gggtgcaccg gcggcccgac ctcgcgcgcg cccatcccga
     1801 acaggtcgac gggcaccgcg gccccgaggc ggccgagcag gtcggtccct gtcacccggc
     1861 cccggcgccg cgcctcgttg acgaccgcgg cggcacgggg aagctcgccg gtgtagcgct
     1921 cgccggggtc gacgacgccg tgctccacca ccctcgtcgg cgtgccgccg cagtcccaga
     1981 acaggtcgtt gaagtgggtg acgtggacga cggtgaggtc gggccggtcg gcggccgggt
     2041 ggcgcagctc ggcgacgcgt ccctgggggg cgttgtgctc gaggtagacg gcggggacgt
     2101 cccggcccgg gc
//
LOCUS       DASPYY010000819         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1683391, whole genome shotgun sequence.
ACCESSION   DASPYY010000819 DASPYY010000000
VERSION     DASPYY010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683391"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VEP73_12810"
     CDS             complement(<1..902)
                     /locus_tag="VEP73_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT27352.1"
                     /translation="MGDRAAAGATPAGAERKLVTVLLADVDEAVEDFAEPDPEDVGRM
                     LARQLARVRAEVEAFGGTVEQTVGGRTVAVFGVPRTRTDDPERAVRAALAIRDALGGG
                     PAGAEVGRLIGRLIRVQVAVATGEALVRGGEPPGGPRQRVLGDPVTACARLLEGQRAG
                     TVLVTEATARATERAIGYAAAQGSEPPGTWYALGPRPGPGPAVPRALPLVARGRELDL
                     LLERFATVRASGSPQLVTVLGPPGIGKSRLVAELAARLRSGAGPVAWRQGRSLPYGDG
                     AAFRPLSAIVRAEAGIGETDPPARA"
     gene            complement(1106..1333)
                     /locus_tag="VEP73_12815"
     CDS             complement(1106..1333)
                     /locus_tag="VEP73_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27353.1"
                     /translation="MLLTRPLRALLAVLTATVLAVATATSAALPRSKHWLALRSKHWA
                     PAWRFANVAALSLAVALLAVIAYQGDRFQWG"
     gene            1692..>2023
                     /locus_tag="VEP73_12820"
     CDS             1692..>2023
                     /locus_tag="VEP73_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27354.1"
                     /translation="MANDEERTAAFERSSEHWLEWLHLELSRRGDTPRSRLLALWDAL
                     EEWFASEDFHGSPLATAPAMPDSGLGAEAVAAHRLAVRQLLEDLAKAAGALDPQGFAV
                     QLQVLVEGA"
BASE COUNT          256 a          759 c          766 g          242 t
ORIGIN      
        1 gcccgggccg gcgggtcggt ctcgccgatg ccggcctcgg cccgcacgat cgccgacagc
       61 ggccggaacg cggccccgtc gccgtacggc agcgaccgtc cctggcgcca ggcgaccggc
      121 cccgccccgc tccggagacg cgccgcgagc tcggccacga ggcggctctt gccgatgccc
      181 ggcggcccga gcacggtgac gagctgcggg gagccgctgg cgcggacggt ggcgaaccgc
      241 tcgaggagca ggtcgagctc tcgcccgcgc gcgacgagcg ggagcgcccg cgggacggcc
      301 ggcccgggcc cggggcgggg gccgagcgcg taccacgtcc cgggtggctc gctgccttgg
      361 gccgcggcgt agccgatggc ccgctcggtg gcgcgcgcgg tcgcctcggt gacgaggacg
      421 gtcccggccc gctgcccctc cagcagccgg gcgcaggccg tgacggggtc gccgagcacc
      481 cgctggcgag ggcccccggg aggctcgccg ccgcggacaa gcgcctcgcc ggtggcgacc
      541 gcgacctgca cccgtatgag ccggccgatg agccgaccga cctcggcgcc ggccggcccg
      601 cctccgagcg cgtcgcggat ggcgagcgcg gcgcgcaccg cgcgctccgg gtcgtcggtg
      661 cgggtgcgcg gcacgccgaa cacggccacc gtcctgccgc cgaccgtctg ctcgaccgtc
      721 ccgccgaacg cctcgacctc ggccctgacc cgcgcgagct gccgggcgag catccgcccg
      781 acgtcctccg ggtcgggctc ggcgaagtcc tcgaccgcct cgtcgacgtc ggcgagcagc
      841 acggtcacga gcttgcgctc ggcacccgcc ggggtggcgc ctgccgcggc gcggtcaccc
      901 atcacggctc ctcggtcgtc cggacgcgga ccggtgggcg cgggccacgc cacgaccggc
      961 tcgggcgtcc taagcgtagc cgccgggcgc cgtggttgtc ccgtccccac gccgccgtgg
     1021 ggacggaccc ggacgcgtac cgggtgcgct ggcggtgatc gccgggcgca cccggttcgg
     1081 ggggtcgcct acctcagggg atcgcctacc cccactgaaa cctgtcgccc tggtacgcga
     1141 tcacggcgag gagcgcgacg gcgagcgaga gggcggcgac gttcgcgaac cgccaggcgg
     1201 gcgcccagtg cttggaccgc agggcgagcc agtgcttgga gcggggcagg gcggcgctgg
     1261 tggcggtcgc aacggccagc accgtggccg tcaggacggc gagcagcgct cgcagcggac
     1321 gagtgagcag catgggcacc tccgggtcgt gtccgtgccc gcctgggggt gggcgtcacc
     1381 cccgccagtg cggcgatgat agagaacgtt atctctcccc acgccgtttg catagaggca
     1441 attcaggaag cggaggcgag gcggcggtgc aatgcggaca gaatgataga gatcgctatc
     1501 actcgtcaat gcccgctcga tgcccgccgg ccgaccctca cgggcggcga cttgcatctg
     1561 gccgcttccg gagagaagga tgtcccctga cctttgcacc tttcgattcg agcgtcgcga
     1621 cccgatccaa gcgtcccgac ccgatccaag cgtcggcgac gcctcaccgc gcagcggtgc
     1681 tccggagctg tatggcgaac gacgaagaac gcacagcggc cttcgagcgg tcgagcgagc
     1741 actggctcga gtggctgcac ctcgagctct cacggcgagg ggacacgccc aggtcgcggc
     1801 tgctcgcgct gtgggacgcg ctggaggagt ggttcgcgag cgaggacttc cacggctcgc
     1861 cgctcgccac cgccccggcc atgccggact ccgggctcgg cgccgaggcg gtcgccgcgc
     1921 atcgcctggc cgtgcgccag ctcctcgagg acctggccaa ggcggccggc gctctcgacc
     1981 cccagggctt cgcggtgcag ctccaggtgc tcgtcgaggg ggc
//
LOCUS       DASPYY010000820         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1965593, whole genome shotgun sequence.
ACCESSION   DASPYY010000820 DASPYY010000000
VERSION     DASPYY010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965593"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VEP73_12825"
     CDS             <1..739
                     /locus_tag="VEP73_12825"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HYT27355.1"
                     /translation="LELVEKARAALSVPVIASLNGVSPGGWTRYAAQLEEAGADALEL
                     NVYYISASPGLSGTEVEWRYLDLVRSVRRTIQIPLAVKVSPYFSSITNLAGRLVEVGA
                     RGLVLFNRFYQPDLDVETLEVVPRLELSTSAELRLPLRWIAILHGRVSASLAASTGVH
                     TAEDAIKALLAGADAVMMTSALLRHGPGHVRVVEAGLRDWLARHGHHRVEEIRGRLSQ
                     RTVGDPTAYERANYLRVLASHPVPRDG"
     gene            complement(991..>1527)
                     /locus_tag="VEP73_12830"
     CDS             complement(991..>1527)
                     /locus_tag="VEP73_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYT27356.1"
                     /translation="KEAIDMTTATATRPRDASLEQALGTAGDAMTSDVLILGAETPAG
                     VAVRRLERAGVSGAPVVQGGRVVGVVTLRDLLIPTALASGQTSGPFLRHEHLLTGLRV
                     SDLMTDEPVVAHLDWPLARVVTAMQEAGVNRVPVVNAPGRPVGILTRDDVIRVLADRL
                     RASPSTSRPVRGTLMAPD"
BASE COUNT          203 a          570 c          555 g          199 t
ORIGIN      
        1 cctcgagctc gtcgagaagg ccagggccgc cctgtcggtc ccggtgatcg cgagcctcaa
       61 cggcgtgtcg cccggcggct ggacccgcta cgcggcccag ctcgaggagg cgggcgccga
      121 cgcgctcgag ctgaacgtct actacatctc agcgagcccg ggcctgagcg ggacagaggt
      181 cgagtggcgc tacctggacc tcgtccgcag cgtgcggagg acgatccaga tcccgctggc
      241 ggtcaaggtg agcccgtact tcagctcgat caccaacctg gccggccggc tcgtcgaggt
      301 cggtgcccgc gggctggtgc tgttcaaccg cttctaccag cccgacctgg acgtcgagac
      361 actcgaggtg gtgccgcgcc tcgagctgag cacctcggcg gagctgcggc tgccactacg
      421 gtggatcgcg atcctccacg gtcgggtctc ggcgtcgctg gccgcgtcca ccggtgtcca
      481 caccgccgag gacgcgatca aggcgctgct ggccggggcc gacgccgtca tgatgacctc
      541 cgcgctgctg cgccacggac ctgggcacgt ccgggtcgtc gaggccggcc tgcgcgactg
      601 gctggcccgt catggccacc accgggtcga ggagatccgc gggcggctga gccagcgcac
      661 ggtcggcgac ccgaccgcgt acgagcgggc caactacctg cgggtgctcg cctcccatcc
      721 ggtcccgcgg gacgggtagg ctggcagatc cgttacaggt gggggtgcgc ccgacagctg
      781 ccggacggcg catggcaccc gccacccgca tcggcggcgc atcctgcgcg tgcgaagccg
      841 acccccggcg ccgtggcggc ctggtgtcgg gtgtctcggt ccagcaccgc aggtcgagca
      901 gggtcacgac gccgacgagg cggtcgccgt ccaccacggt cgcctcgcgg gcctgggcgg
      961 acgcagccgg tcgacggcca gagccccgag ctagtccggg gccatcaggg tgccgcggac
     1021 aggccgcgag gtgctgggcg acgcccgcag gcggtcggcg agcacgcgga tcacgtcgtc
     1081 ccgggtgagg atgccgaccg gccggcccgg ggcgttgacc acaggcacgc ggttcacccc
     1141 ggcctcctgc atcgcggtga cgacccgcgc caacggccag tccaggtggg cgacgaccgg
     1201 ctcgtcggtc atcagatcgc tcacgcgcaa ccctgtgagc aggtgctcgt ggcgcaggaa
     1261 cggcccggag gtctgccccg acgccagcgc ggtcgggatc agcaggtcgc gcaacgtgac
     1321 cacgcccacc acccgcccgc cctgcacgac cggggcgccc gacacgcccg cgcgctcgag
     1381 ccggcgcacg gccacgccgg ccggcgtctc ggcgccaagg atcaggacgt cgctggtcat
     1441 cgcatcgccc gcggtcccga gggcctgctc gaggctcgcg tcgcgtggcc gcgtcgccgt
     1501 cgccgtcgtc atgtcgatcg cctcctt
//
LOCUS       DASPYY010000821         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2104932, whole genome shotgun sequence.
ACCESSION   DASPYY010000821 DASPYY010000000
VERSION     DASPYY010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104932"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VEP73_12835"
     CDS             complement(<1..1662)
                     /locus_tag="VEP73_12835"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HYT27357.1"
                     /translation="MRTRGLAAARGRAVWMHRAVTVAAFVGIYLILVKVLHLNLILSR
                     TTAAGGDMGSHHYIDTFLREHLLPHGQVTGWATGWFAGIPMLTFYFPLPYVLIAALTP
                     LLGNQIAFKLVTASGLFLLPLTCWGAFRVLRLPEPAPLLAAAASVPFLFMTSYTIYGG
                     NIASTMAGEFPFALSFALLPLALALLWRLAETGAGWRVTALLVTAVVLSHILTTIVLM
                     LGATILVLRPGWRATAVAVGRLARVFAVAFCLAAFWALPFIVRTQYTAHFKWTQLSSY
                     GVLFPLEIRPYLVLSAVGLVVAAARGERRILLYAWPCVVAVCLFAFLSRYAPKAALWN
                     ARMLPFIYLFALLVAAYGAAAVARRLTGLLQRGIGVPARAAWLVVVAALVAAPLYGSW
                     RHRGFTPGWAQYNYEGFEVKPGWPQAEALFSTLRALPKGRVMWEFSRDYESFGTTRTL
                     ENIPVFAGQDSMEGLLIESSLNAPFHFINQAETSQTQTQAVPGIDYPSFDFPMGLRHL
                     RMYGVRWYVAYTDQAKQAAKAAKLPVRATSGKFTVFEVGDGHLVEV"
     gene            1746..>2963
                     /locus_tag="VEP73_12840"
     CDS             1746..>2963
                     /locus_tag="VEP73_12840"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HYT27358.1"
                     /translation="MVTPLRVAEATDRGPLPPAGTWLWAGELDLDPERTADVSFDRDG
                     RALLSLVLDRPATDADVEALERAADVLPHLRLGDLDGGAGYPERHAAAFGLRRPLAAT
                     TFNRAATVELEAPSGRRLPPAIGTFGLPARTTLYAAPAPAPARPRRAPVGSRIEHRLL
                     GAGRLLVARTRLSASTLCGLALAASLAVFAVAFVRQTWALHDRFGTYGFDVGIYDQGT
                     WLLSRFHAPFSTVRGLNLFGDHASYVLVLLAPLYRLWADPRLLLALQVVFLAVPAVVV
                     YRLGARRLGHPAAGLAVAVAYLAYPAMQWAIVWQFHPETLAAGFLALAALAADRGRTR
                     AMAVLLALALLCREDVGLVVAGFGALLAVTGRRALGMRTALAGLAWFLVTTFLLVPLA
                     NGRLTAQFEQTYGV"
BASE COUNT          407 a         1113 c         1054 g          389 t
ORIGIN      
        1 cacctcgacc aggtgcccgt cccccacctc gaagacggtg aacttgccgc tggtggcccg
       61 gaccgggagc ttggcggcct tggccgcctg cttcgcctgg tcggtgtagg ccacgtacca
      121 tcgcacgccg tacatgcgca ggtggcgcag gcccatcggg aagtcgaagc tcgggtagtc
      181 gatccccggg accgcctgcg tctgggtctg ggatgtctcg gcctggttga tgaagtggaa
      241 cggggcgttc agcgacgact cgatcagcag tccctccatc gagtcctggc cggcgaagac
      301 ggggatgttc tcgagcgtcc tcgtcgtgcc gaagctctcg tagtcgcgac tgaactccca
      361 catgacccgg cccttgggca gcgcccgcag cgtggagaac aacgcctcgg cctgcggcca
      421 gcccggcttc acctcgaatc cctcgtagtt gtactgggcc cagcccggcg tgaacccgcg
      481 gtggcgccag gagccgtaca ggggcgcggc gaccagggcg gcgaccacga cgagccaggc
      541 cgcgcgggcg ggcacgccga tgccccgctg cagcaggccg gtcagccgcc tggcgacggc
      601 cgccgccccg tacgcggcca ccagcagcgc gaacaggtag atgaacggca gcatgcgcgc
      661 gttccacagc gccgccttgg gcgcgtagcg ggacaggaac gcgaacaggc agaccgccac
      721 cacgcagggc caggcgtaga gcaggatgcg ccgctcgccg cgcgcggccg ccacgaccag
      781 gccgaccgcg ctgagcacca ggtacggccg gatctcgagc gggaacagca ccccgtacga
      841 cgagagctgg gtccacttga agtgggcggt gtactgggtc cggacgatga acggcagcgc
      901 ccagaacgcg gccaggcaga acgcgaccgc gaacacccgc gcgagccggc cgaccgccac
      961 ggcggtggcc cgccagccgg gccgcagcac caggatcgtg gccccgagca tgagcacgat
     1021 cgtcgtgagg atgtgcgaca gcaccaccgc ggtgaccagc agcgcggtga cgcgccagcc
     1081 ggcgcccgtc tcggcgagcc gccacagcag cgccagcgcg agcggcagca gggcgaacga
     1141 cagggcgaag gggaactccc cggccatcgt gctcgcgatg ttgccgccgt agatggtgta
     1201 ggaggtcatg aacaggaacg gcacgctggc cgcggccgcg agcagcgggg ccggctcggg
     1261 gagccgcagc acccggaacg cgccccagca ggtcaggggg agcaggaaca gcccggacgc
     1321 ggtcaccagc ttgaacgcga tctggttgcc gaggagcggg gtgagcgcgg cgatcagcac
     1381 gtagggcagc gggaagtaga aggtgagcat cgggatgccc gcgaaccagc cggtcgccca
     1441 gccggtcacc tggccgtggg gcagcaggtg ctcgcgcagg aacgtgtcta tgtagtggtg
     1501 cgagcccatg tcgccccccg ccgcggtggt gcgagagagg ataaggttca ggtgcagcac
     1561 cttgacgagg atgaggtaga tgccgacgaa cgcggccacc gtgacggcgc ggtgcatcca
     1621 caccgcgcgg ccacgggccg cggccagccc ccgggttcgc accgccgcga tcgtcgccag
     1681 ccgactctcc tcctcggacc gttgttcgcg cgtcgttggc tcgcacttat gaaagcagga
     1741 tgggcatggt aacgccgctc cgggtcgcgg aagcgaccga caggggcccc ctccccccgg
     1801 ccggcacctg gctgtgggcc ggtgagctcg acctggaccc cgagcggacc gccgacgtgt
     1861 cgttcgaccg cgacgggcgg gcgctgctct ccctcgtcct cgaccggccc gccaccgacg
     1921 ccgacgtcga ggcgctcgag cgggcggccg acgtcctccc ccacctgcgg ctcggggacc
     1981 tcgacggcgg tgccggctac cccgagcggc acgcggccgc gttcggcctg cgccgccccc
     2041 tggccgcgac cacgttcaac cgcgccgcca cggtcgagct cgaggcaccc tccggccgcc
     2101 gcctgccccc ggccatcggt acgttcgggc tgcccgcgcg gacgacgctc tacgcggctc
     2161 cggcgcccgc ccccgcccgg ccgcgtcggg cgccggtcgg ctcgaggatc gagcaccgcc
     2221 tgctcggcgc cgggcgcctc ctcgtggcca ggacccggct gtcggcctcg accctgtgcg
     2281 gcctcgcgct cgccgccagc ctcgccgtgt tcgccgtcgc gttcgtgcgc cagacctggg
     2341 cgctgcacga ccgcttcggc acctacggct tcgacgtcgg catctacgac cagggcacct
     2401 ggctgctcag ccggttccac gcgcccttct cgaccgtccg ggggctgaac ctgttcggcg
     2461 accacgcctc ctacgtgctc gtgctgctcg cgcccttgta ccggctgtgg gccgaccccc
     2521 gcctgctgct cgcgctccag gtggtgttcc tcgccgtgcc cgccgtggtc gtctaccggc
     2581 tcggcgcccg ccggctcggc catccggccg ccggcctggc cgtcgccgtc gcctacctcg
     2641 cctacccggc gatgcagtgg gcgatcgtgt ggcagttcca ccccgagacg ctcgcggccg
     2701 ggttcctggc cctggccgcg ctcgccgccg accgggggcg gacacgggcc atggccgtcc
     2761 tgctcgccct ggccctgctc tgccgggagg acgtcggcct ggtcgtggcc gggttcggag
     2821 cgctcctcgc cgtcaccggc cggcgggcgc tcggcatgcg caccgcgctc gcagggctgg
     2881 cctggttcct cgtgaccacg ttcctgctcg tccccctggc caacgggcgg ctgaccgccc
     2941 agttcgagca gacctacggc gtg
//
LOCUS       DASPYY010000822         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_417894, whole genome shotgun sequence.
ACCESSION   DASPYY010000822 DASPYY010000000
VERSION     DASPYY010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417894"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VEP73_12845"
     CDS             <1..814
                     /locus_tag="VEP73_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT27359.1"
                     /translation="TTLTLSGRRLTADDRRVLRAFTDQLKVALESRRLADAAATATAL
                     EEANNLRTALLAAVSHDLRTPLASIKTSVTSLLQGDVDWAPEATSQFLTTIDQEADRL
                     NTLVGNLLDMSRLQTGALNVLGRPVGLEEVVPAALAGLADHGRSVQVDVPETLARVYA
                     DPALLERAIANVVANAVTWSPPDRPVRLTASRAGDRIDLRVVDHGPGIPPAGRERVFQ
                     PFQRLGDAAGGTGVGLGLAVARGFVQAMGGQILLEDTPGGGLTMRINLAAAT"
     gene            828..1511
                     /locus_tag="VEP73_12850"
     CDS             828..1511
                     /locus_tag="VEP73_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT27360.1"
                     /translation="MSRVLVVDDEPQILRALATNLNARGYEVDLAPTGEAALAQAARH
                     HPDAVILDLGLPGIDGLEVIRGLRGWSRMPIIVLSVREREADKIAALDAGADDYVTKP
                     FGIGELLARLRAAVRRAIPAEEAAVVHTADFVVDLASKQVSTRHGEVRLTPTEWHLVE
                     VLVRNQGRLVSQRQLLQEVWGPQYRDETNYLRVFMAQVRRKLEPNPAAPRYFITEPGM
                     GYRFQPGTA"
BASE COUNT          229 a          701 c          551 g          194 t
ORIGIN      
        1 caccaccctc accctgagcg ggcgccgcct gaccgccgac gaccgccggg tcctgcgtgc
       61 cttcaccgac cagctcaagg tcgcactgga gagccgccgg ctcgccgacg ccgccgccac
      121 cgccaccgcg ctggaggagg ccaacaacct gcgcaccgcc ctgctggccg cggtctccca
      181 cgacctgcgc acgccgctcg cctccatcaa gacctcggtg accagcctgc tccaaggcga
      241 cgtcgactgg gcacccgagg cgaccagcca gttcctgacc accatcgacc aggaggccga
      301 ccggctcaac acgctggtcg gcaacctgct cgacatgagc cggctgcaga ccggggcgct
      361 caacgtgctc ggccgccccg tcggcctcga agaggtcgtg cccgccgcgc tcgccggcct
      421 cgccgaccac ggccggagcg tgcaggtcga cgtgccggag accctcgccc gcgtctacgc
      481 cgaccctgcc ctgctggagc gggccatcgc caacgtcgtc gccaacgcgg tcacctggtc
      541 cccgcccgac cggcccgtcc ggctcaccgc cagccgcgcc ggcgaccgca tcgacctgcg
      601 cgtcgtcgac cacggtccgg ggatcccccc cgccggccgc gagcgcgtct tccagccctt
      661 ccagcgcctg ggcgacgcgg ccggcggcac cggcgtcggc ctcggcctcg ccgtcgcccg
      721 cggcttcgtg caggccatgg gcgggcagat cctcctcgag gacaccccgg gcggcgggct
      781 caccatgcgg atcaacctcg cggccgcgac atgaccgccg cccgccgatg agccgcgtgc
      841 tggtcgtcga cgacgaaccg cagatcctgc gggcgctcgc caccaacctc aacgcccgcg
      901 gctacgaggt cgacctggcc cccacgggcg aggccgccct cgcccaggcc gcccgccacc
      961 atcccgacgc ggtcatcctc gacctcggcc tgcccggcat cgacggcctg gaggtgatcc
     1021 gcggcctgcg cgggtggagc aggatgccga tcatcgtgct gtcggtccgc gagcgcgagg
     1081 ccgacaagat cgcggccctc gacgcgggcg ccgacgacta cgtcaccaag ccgttcggga
     1141 tcggcgagct gctcgcccgg ctccgcgcgg ccgtccgccg cgcgatcccc gccgaggagg
     1201 ccgccgtcgt ccacacggcc gacttcgtgg tcgacctcgc cagcaagcag gtgagcaccc
     1261 gccacggcga ggtccggctc acccccaccg agtggcacct cgtcgaggtc ctcgtccgca
     1321 accagggcag gctcgtcagc cagcggcagc tcctccagga ggtctggggg ccgcagtacc
     1381 gcgacgagac caactacctc cgcgtgttca tggcccaggt ccgccgcaag ctcgagccca
     1441 acccggccgc gccccgctac ttcatcaccg agccgggcat gggctaccgc ttccagccgg
     1501 gcaccgcctg accgcggacc ggcgggcgcg ccggagccgg accgggacgg tgcgtgccag
     1561 gctcagttcg tggtcgaccg ggcgagctcg ccgccgagtg cgtcggcgcg gtcgcgccat
     1621 ggcagccccc cagggtgtgg ccggccgcgg gcgacggaca ccggctactt cacca
//
LOCUS       DASPYY010000823         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_1684732, whole genome shotgun sequence.
ACCESSION   DASPYY010000823 DASPYY010000000
VERSION     DASPYY010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684732"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VEP73_12855"
     CDS             <1..853
                     /locus_tag="VEP73_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27361.1"
                     /translation="RQALRCGHVPLLLGAEGEDSSPRTLPDFRRLLATRITQVRGWMG
                     LPPAGPSQLNLLARFDRDRVVDDRLAWEIRDLLDLRGEVTPEVVRIAVETDLGRLAAD
                     ARERHPQVARAGGHAVLLLDGVDDNSVDLLGVLHDEILGDHGLGTRAEPVPVVVAMTS
                     SDQANLLRDLAEGHTDKPWLDLRTLRPFRGDGEDLLAYQQVMLHPFRKEPAGIADVPW
                     VFNRTLAPALWDGYVKALRLTLKGWPSAFTGDLFTVLLWAQVGGFVQRADDWDLIKRF
                     REELRQP"
     gene            850..>2309
                     /locus_tag="VEP73_12860"
     CDS             850..>2309
                     /locus_tag="VEP73_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27362.1"
                     /translation="MTQGTEARADALAAELLAQLQRSQAARLDELGGEPLTWLAVAPL
                     WTDRLARACGFPEHPLPLPEFLTRAETAGWCARRPGERRARTPHLRLQVQAELLPHLS
                     AAEQRAALDEVLGIEGEVERAALLALLVPNLPADLLAEVSDAIWGSSGAASVPVLLAL
                     AVRAQPPLRSRVIRELTGRIAEIEDPEERAARLVELSQVLPDEIPAATTRLTWEQLVE
                     GERPPLRRSRREDLICRLVPWVALVSPGPVEEEMRWRLREHELAAAARLLRAADNRLD
                     LQATIQFFEQLSQSVRGLPAGIERDLALDDLTTTAVAAPYLSHRSALIYLALVEEIAD
                     VRIRLHALTELTALNEPDAPEWHTDVEARWVTAATRPDGTLDLVAVARHVERHADALH
                     REHVREHAHLAAGQAGRTSPSPVVVAALQQIAQSLGPRNQETAVLARGLLDAAADLAR
                     RLGDAVPRAAALTSTARLYLEVIGALPAHPALDEPAL"
BASE COUNT          294 a          924 c          787 g          304 t
ORIGIN      
        1 ccgccaggcg ctccgctgcg ggcacgtccc cctgctgctc ggcgccgagg gcgaggacag
       61 ctcgccgcgc accctgcccg acttccgccg cctgctcgcc acccggatca cccaggtgcg
      121 cggctggatg gggctgccgc cggctggtcc gagccagctc aacctgctgg ccaggttcga
      181 ccgcgaccgg gtggtcgacg accggctcgc ctgggagatc cgcgacctcc tcgacctgcg
      241 gggcgaggtc acccccgagg tggtccgcat cgccgtcgag accgacctgg gccgcctcgc
      301 cgccgacgcc cgcgagcgcc atccccaggt cgcccgtgcg ggcggccacg cggtgctgct
      361 gctcgacggg gtggacgaca actcggtgga cctgctcggc gtgctccacg acgagatcct
      421 cggcgaccac ggcctcggca cccgcgccga gccggtcccg gtggtcgtcg ccatgacctc
      481 ctccgaccag gcgaacctgc tgcgcgacct cgccgagggc cacaccgaca agccctggct
      541 cgacctgcgg accctgcgcc cgttccgggg cgacggcgag gacctgctcg cctaccagca
      601 ggtgatgctc cacccgttcc gcaaggagcc agccgggatc gccgacgtgc cctgggtctt
      661 caaccggacc ctcgccccgg ccctctggga cggctatgtg aaggcgctgc gcctgaccct
      721 caagggctgg ccgagcgcct tcaccggcga cctcttcacc gtcctgctct gggcgcaggt
      781 cggcgggttc gtgcagcgcg ccgacgactg ggacctcatc aagcggttcc gcgaggagct
      841 gcggcagcca tgacccaggg gaccgaagcc cgcgccgacg cgctcgccgc cgagctgctc
      901 gcccagctcc agcgcagcca ggccgcacgc ctcgacgagc tgggcggcga gccgctcacc
      961 tggctggcgg tcgccccgct gtggaccgac cgcctcgccc gcgcctgcgg cttccccgag
     1021 caccccctcc cgctgcccga gttcctcacc cgcgccgaga ccgccggctg gtgcgcccgc
     1081 cgcccgggcg agcgccgcgc acgcaccccg cacctgcgcc tccaggtcca ggccgagctg
     1141 ctcccgcatc tgtccgccgc cgagcagcgc gccgcgctcg acgaggtgct cgggatcgag
     1201 ggggaggtcg agcgcgccgc gctcctcgcc ctcttggtgc cgaacctgcc ggccgacctg
     1261 ctcgccgagg tgagcgacgc catctggggc agcagcggcg ccgcctccgt gccggtgctc
     1321 ctggcgctgg cggtcagggc gcagccgccg ttgcggtcaa gggtgatccg tgaactgact
     1381 ggccgcatcg cggagatcga ggatccggag gagcgtgccg cccgcctggt cgagttgagc
     1441 caggtcctgc cggacgagat tcccgcggcc accacccggc tgacctggga gcaacttgtc
     1501 gagggcgagc gacctccgct gcggcggtcg cgccgcgagg acctcatctg ccgcctggtc
     1561 ccatgggtgg cgctggtatc gccgggcccg gttgaggaag agatgcgctg gaggttgcgg
     1621 gaacatgagc tggcagctgc ggcgcggctc ctccgcgcgg ctgacaaccg gcttgacctc
     1681 caggcgacca tccagttctt cgagcagctg tcccagagcg tccggggcct tcccgccggg
     1741 atcgagcgcg acctcgccct cgacgacctg accaccacag cggtcgcggc gccgtacctc
     1801 tcccaccgat ccgcgctcat ctatctggcc ctcgtcgaag agatcgccga cgtccgcatc
     1861 cggctccatg cactgaccga actgacggcg ctgaacgagc cggacgcccc ggagtggcat
     1921 accgacgtcg aggcccgctg ggtgacggcg gcgactcgcc ctgatgggac gctcgacctg
     1981 gtggcggtcg cccgccacgt cgaacgccat gccgacgccc tgcaccggga gcacgtccgc
     2041 gagcacgccc acctggccgc cggtcaggcg ggccgcacca gtccctcacc ggtggtggtc
     2101 gccgcgctcc agcagatcgc ccagtcgctc ggaccacgca accaggagac cgccgtcctc
     2161 gcccgcgggc tcttggatgc ggcggccgac ctcgcccggc ggctcggcga cgccgtccca
     2221 cgcgcggccg cgctcaccag caccgcgagg ctctacctcg aggtcatcgg ggcgcttccc
     2281 gcgcaccccg cgctggacga gccggcgct
//
LOCUS       DASPYY010000824         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_2667544, whole genome shotgun sequence.
ACCESSION   DASPYY010000824 DASPYY010000000
VERSION     DASPYY010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667544"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2222
                     /locus_tag="VEP73_12865"
     CDS             <1..>2222
                     /locus_tag="VEP73_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HYT27363.1"
                     /translation="LLAVGAVQHHLLRAGLATRTSIVAATGEPCDSHQAAALLGYGAD
                     AICPRLTLATVAELVDGDPAGAQERYLEALEEGVYKVMSKMGISTLDSYRGAQIFEVL
                     GLDAEVVDACFAGTASPLGGVGFDDLAADVLTRHHLARGGAPQLDNPGWFKHRPRGEY
                     HATNPQVMQALHFAVRGDDAEMKGSKRGAHLLQQAVKGGGYERYEHFAKLVNQRPPTA
                     LRDLLEPLPAGPAVPLEQVEPAGAILRRFSTAAMSLGSLSPEAHQTLAIALNRVGGRS
                     NSGEGGEDPARFGTQRSSAIKQVASGRFGVTPEYLANAAELQIKIAQGSKPGEGGQLP
                     GHKVSAEIARLRHTQPGVALISPPPHHDIYSIEDLAQLVFDLKQANPEADVTVKLVAE
                     AGVGTVAAGVVKSLADVVTISGADGGTGASPLSSIKHAGAPWELGLAETQQALRANGL
                     RSRVRVRVDGGFKTGRDVLLAALLGADEYGFGTAALLAEGCLMVRTCHQDNCPVGIAT
                     QRPELRAKFTGTPDMVVHYLEYVAEEVRRLLASLGLRTLDEAIGRVDLLRQRASGNPR
                     ADRLDLGPLLADPVPADRGAGGDRVGQGSGEPLRFVAPDLGMQRPRSALGDAVHDDAM
                     PGLLAGDLVNLRYPIANTDRAVGARLGVAIGAAYGSRPPAGRARVEFDGTAGQSFGAF
                     LAPGVDFRLTGSANDYVGKGMGGGRIVIRPPADDAGAPVLVGNTALYGATRGELFCAG
                     "
BASE COUNT          287 a          876 c          791 g          268 t
ORIGIN      
        1 ctcctcgccg tcggcgcggt gcagcaccac ctgctgcggg cggggctcgc cacgcgcacg
       61 agcatcgtcg cggccaccgg cgagccatgc gactcccacc aggcggccgc gctgctcggc
      121 tacggcgccg acgcgatctg cccgcgcctc acccttgcca cggtggccga gctcgtcgac
      181 ggcgacccgg ccggcgccca ggagcgctac ctcgaggcgc tcgaggaggg cgtctacaag
      241 gtcatgtcga agatggggat ctccaccctc gactcctacc ggggggcgca gatcttcgag
      301 gtcctcgggc tcgacgccga ggtggtcgac gcctgcttcg cggggacggc gtcgccgctc
      361 ggcggcgtcg ggttcgacga cctcgccgcc gacgtgctca ccaggcacca cctggcccgg
      421 ggcggggcgc cccagctcga caaccccggc tggttcaagc accgcccgcg cggcgagtac
      481 cacgccacga acccgcaggt catgcaggcg ctgcacttcg ccgtgcgcgg tgacgacgcc
      541 gagatgaagg ggtccaagcg cggcgcccac ctgctccagc aggcggtcaa gggcggcggc
      601 tacgagcgct acgagcactt cgccaagctc gtgaaccagc ggccgccgac cgccctgcgc
      661 gacctcctcg agccgctccc cgccggcccg gccgtcccgc tcgagcaggt ggagccggcc
      721 ggcgccatcc tccggcgctt ctcgaccgcc gcgatgagcc tcgggtcgct gtcgccggag
      781 gcccaccaga ccctggcgat cgcgctcaac cgggtcggcg gccgctcgaa ctccggcgag
      841 ggcggcgagg acccggcccg cttcggcacg cagcgcagct ccgccatcaa gcaggtcgcc
      901 tcgggccgct tcggggtcac ccccgagtac ctggccaacg cggccgagct gcagatcaag
      961 atcgcccagg gctccaagcc cggcgagggc gggcagctgc ccggccacaa ggtcagcgcc
     1021 gagatcgccc gcctgcgcca cacccagccg ggggtggcgc tgatctcgcc gccgccgcat
     1081 cacgacatct actccatcga ggacctcgcc cagctggtct tcgacctgaa gcaggccaac
     1141 ccggaggctg acgtcaccgt caagctggtc gccgaggcgg gggtcggcac ggtcgccgcc
     1201 ggggtggtca agtccctggc cgacgtggtc acgatatcgg gagctgatgg cggcaccggc
     1261 gcgagcccgc tgtcgtcgat caagcacgcc ggcgcgccgt gggagctcgg gctggccgag
     1321 acccagcagg ccctgcgggc caacgggctg cggtcgcggg tgcgggtgcg ggtggacggc
     1381 ggtttcaaga ccggcaggga cgtgctgctc gcggcgctcc tcggcgccga cgagtacggc
     1441 ttcggcaccg ccgcgctcct cgccgagggc tgcctcatgg tccgcacctg ccaccaggac
     1501 aactgcccgg tcgggatcgc cacccagcgc ccggagctgc gggcgaagtt caccggcacc
     1561 cccgacatgg tggtccacta cctggagtac gtcgccgagg aggtccgccg cctgctcgcc
     1621 tcgctcggcc tgcgcaccct tgacgaggcg atcggccggg tcgacctgct caggcagcgg
     1681 gcgagcggca accccagggc cgaccggctc gacctcggcc cgctcctggc cgacccggtc
     1741 cccgccgatc ggggggccgg cggggaccgg gtcggccagg ggtccggcga gccgctccgg
     1801 ttcgtcgccc ccgacctggg catgcagcgc ccccgctcgg ccctcggcga cgcggtgcac
     1861 gacgacgcca tgcccggcct cctcgccggg gacctcgtca acctgcgcta cccgatcgcc
     1921 aacaccgacc gggcggtcgg cgcgcgcctc ggggtcgcca tcggcgccgc ctacggctcc
     1981 cgcccgcccg ccggccgggc cagggtcgag ttcgacggca ccgccgggca gagcttcggc
     2041 gcgttcctcg cccccggggt cgacttccgg ctcaccggca gcgccaacga ctacgtcggc
     2101 aaggggatgg gcggcggccg catcgtcatc cgcccgccgg ccgacgacgc gggcgccccg
     2161 gtgctcgtcg gcaacaccgc cctgtacggg gcgacccggg gggagctgtt ctgcgccggc
     2221 tg
//
LOCUS       DASPYY010000825         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_280486, whole genome shotgun sequence.
ACCESSION   DASPYY010000825 DASPYY010000000
VERSION     DASPYY010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280486"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VEP73_12870"
     CDS             <1..305
                     /locus_tag="VEP73_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYT27364.1"
                     /translation="GMMEARIRQLEEILRSAVVGAPRDTGKAGPGMIVRLEIAGDEET
                     YFLGSREEAIEGMDILSLQSPLGQAVVGRRPGEEFSYLTPTGRELPVKLLEAEPRR"
     gene            complement(329..1222)
                     /locus_tag="VEP73_12875"
     CDS             complement(329..1222)
                     /locus_tag="VEP73_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYT27365.1"
                     /translation="MVGAELEPRYRVTAVEAMTGVPASTLRSWERRYGWPHPVRTASD
                     QRLYSDHDVAVVRFLVQRRTEGISMAQAAGLLERTAAADERDPERILARLVAALRAFD
                     EPAAEATFTAAERLLGPEGAARELVPRAIREVAGEVGGDLPVAAEHFASNLLRRQVLR
                     LLGDLPPARGRALLVGCAPDEEHELGALLLAMLLRVRGHRVVYLGSRLPGAALEHAVA
                     ALDPTGVAISITMPDCLEQAVAWARTSGRRPGAGPRYWWGGPAVAQAPELASRLPAEV
                     LDTSAADGASRITAALSTRRR"
     gene            complement(1275..>1525)
                     /locus_tag="VEP73_12880"
     CDS             complement(1275..>1525)
                     /locus_tag="VEP73_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HYT27366.1"
                     /translation="YDPARIHLATVDAARIAATAARLAGMTIAERAALPVMAPGREDV
                     IAAGALLLAEICELLGFMRITSSEADILDGVVLGLAGG"
BASE COUNT          195 a          538 c          611 g          181 t
ORIGIN      
        1 agggcatgat ggaggcgcgg atccgccagc tcgaggagat cctccgctcg gcggtcgtcg
       61 gggcgccacg ggacaccggc aaggctggac ctggcatgat cgttcgcctg gagatcgccg
      121 gcgacgagga gacgtacttc ctcggcagcc gcgaggaggc gatcgagggc atggacatcc
      181 tcagcctgca gtcgcctctc ggccaggcgg tcgtcggccg gcggcccggc gaggagttca
      241 gctacctgac cccgaccggt cgtgagctgc cggtcaagct gctcgaggcc gagccgcgcc
      301 gctgaccggg tccccgcgcg gagttcggtc agcggcgccg ggtcgagagg gcggcggtga
      361 tcctggaggc gccgtcggcg gcggacgtgt cgagcacctc ggccgggagg cggctcgcga
      421 gctcgggggc ctgggcgacg gccgggccgc cccaccagta gcgggggccg gcgccaggcc
      481 gccggccgct cgtccgggcc caggcgaccg cctgctcgag gcagtccggc atggtgatgc
      541 tgatcgcgac gccggtcggg tcgagcgcgg cgacggcgtg ctcgagcgcc gcgccgggca
      601 ggcgggagcc caggtagacc acccggtgcc cgcggacgcg cagcagcatg gcgaggagca
      661 gcgcgcccag ctcgtgctcc tcgtcggggg cgcagccgac gagcagggct cgcccccgcg
      721 cgggcgggag gtcgccgagc agccggagca cctgcctgcg gaggaggttg gaggcgaagt
      781 gctcggcggc gacggggagg tcgccgccga cttcgccggc gacctcgcgg atcgcgcggg
      841 ggaccagctc gcgggccgcg ccctcgggac cgagcagccg ctcggcggcg gtgaacgtcg
      901 cctcggcggc gggctcgtcg aaggcccgga gcgcggcgac gaggcgggcg aggatgcgct
      961 cggggtcgcg ctcgtcggcg gcggccgtcc gctcgagcag gccggcggcc tgcgccatcg
     1021 agatcccctc ggtcctgcgc tggaccagga agcgcacgac ggcgacgtcg tggtcggagt
     1081 agagccgctg gtcggacgcg gtgcgcacgg ggtggggcca gccgtagcgc cgctcccacg
     1141 agcgtagggt gctggccggg acgccggtca tcgcctccac cgccgtgacc cggtagcgag
     1201 gctcgagctc ggccccgacc accggcggct ccgttgccga gcttcccctc cccatgctca
     1261 cctcccggcg gggatcatcc tcctgccagc ccgagcacaa cgccgtccag gatatctgct
     1321 tcactcgacg tgattcgcat gaatccgagc aattcacaga tctccgcaag aaggagcgcc
     1381 ccggcggcga tgacgtcctc gcggccgggg gccatgaccg gcagcgcggc gcgctcggcg
     1441 atggtcatcc cggcgagcct ggcggcggtg gcggcgatcc gcgccgcgtc gacggtggcg
     1501 aggtggatcc gcgccgggtc gtagc
//
LOCUS       DASPYY010000826         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11605
            k141_420089, whole genome shotgun sequence.
ACCESSION   DASPYY010000826 DASPYY010000000
VERSION     DASPYY010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945335
            Sequence Read Archive: SRR6266571
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 07:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,581
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 10 of 46
            Pseudo Genes (incomplete)         :: 40 of 46
            Pseudo Genes (internal stop)      :: 0 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420089"
                     /isolate="SMAG_U11605"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VEP73_12885"
     CDS             <1..1522
                     /locus_tag="VEP73_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYT27367.1"
                     /translation="VGYIAAHCEAKVAIVEDLGFLERFEKVREQLPALRRVVLVEDAD
                     TVAGQDQVVSWERLRAAGRDALAAGRDVFEAAWKRAQPEDAATLIYTSGTTGPPKGVV
                     ITHRNALWTAASVERSWPEPIAPGTPYLSYLPLAHSYERLCGHYACLWSAQQVHFCPD
                     ILRVMEYLPEVRPVSFVAVPRLWEKAYAGITAAMAAEPNERKRRLVTRALAVGREAVR
                     LEQAGKPVPAGLKLQRRLFDRLVFSRIRARIGLDRCRVAVSGAAPIAGEVLEFFLAVG
                     LPMVEGYGLSENTAGATVNPVGRTRVGTVGLPLPGVEVRLDDDGEILVRGGNVTAGYF
                     KEPEKTAETFDAEGWLHTGDIGSLDDDGFIRVVDRKKELIVTAGGKNISPANLETLLK
                     RHPLVGQACVIGDRRPFLSALVVLDPETAPAWAKRHGLPTTRIEDLAAEERVVAEVQR
                     AVDDVNEHVSQVERVKKFVVLPAEWAVDSEELTPTLKLKRRVIVEKYAAEIESLYA"
     gene            complement(1595..2296)
                     /locus_tag="VEP73_12890"
     CDS             complement(1595..2296)
                     /locus_tag="VEP73_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT27368.1"
                     /translation="MMRQRQPHAAIADRSATIDRLTSGLYRLDTAQLYDQVFAHLARI
                     DPAVMFERVFANLSRLRSAQASGQAFSALRISDAWPVMEWTMANLSRLETARLYGQVS
                     VTLSKVANGFQAAGAFMVLLHEGVVELGWFMDERAGDPVAEAVARDLGRLAGSLPAGP
                     PPASDVAACWDPWFRTGLFIAAALLVAADRPAGDGGGVRAARMVDTAAGLVSLVLAYD
                     AFRGVAAQPLRSMPA"
     gene            2530..3489
                     /locus_tag="VEP73_12895"
     CDS             2530..3489
                     /locus_tag="VEP73_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYT27369.1"
                     /translation="MIDFARVLRAAGVPVREVAGWRQRGRPPSSNPRDPAWPAATKFA
                     GIGLHHTAGHNDLSVVVNGRPGLPGPLANLYVARDGMVYVVAAGRANHLGQGASEVYR
                     RTLRDLAPLGDAAALGLADSMVGNGVYAGIEVENYGTPGDPYPGVQIASLEQACAALC
                     RTAGWTANRCVQHREWTRRKVDMSYRGPLRAVVAAMLSEEDLILDAQTRAYFDAQFEL
                     LRLGDNPDPPTGDTHPQSFEGVLRRLDRIEALLEIVWRGDQPDPATGDTHPQNLETIW
                     RQGQATTTALQAQAIDVTAIRTDVEAIRRAVEAMRPSGGAAGR"
     gene            3486..4337
                     /locus_tag="VEP73_12900"
     CDS             3486..4337
                     /locus_tag="VEP73_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT27370.1"
                     /translation="MTGRRGGDGAERGRPAAGAGLDLDGSVALVTGGASGIGLAVVGR
                     LAAAGARVVVADLDEAGGAAAAAAAGGVFVATDAGDPDAVQAAVDASEAAYGGLDLVH
                     LNAGIVTGNAALDLLDLDRYRRVVAVNLDGVVFGVRSALPALRRRGGGAIVATASLAG
                     LTAYPGDPVYSMTKHGVVGLVRTLAEPLAADRVTINCVCPGFADTPLLDRFAGQLRTA
                     GFPLLSADEVAAAVLTAATGGGTGQAWVCQPGRPPEPFRFRGVPGPRVAGSEGIQPPP
                     TEPGGPS"
     gene            4453..>4670
                     /locus_tag="VEP73_12905"
     CDS             4453..>4670
                     /locus_tag="VEP73_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT27371.1"
                     /translation="MRPTVLIVDDHAAFRASARALLQAEGFDVVGEAADAAGAVEAVA
                     ALRPEIVLLDVQLPDLDGLAVAEQLAGA"
BASE COUNT          627 a         1740 c         1723 g          580 t
ORIGIN      
        1 ggtcggctac atcgccgccc actgcgaggc caaggtggcc atcgtcgagg acctcgggtt
       61 cctcgagcgc ttcgagaagg tgcgcgagca gctccccgcc ctgcgccgcg tcgtgctggt
      121 ggaggacgcc gacaccgtcg ccggccagga ccaggtcgtg tcgtgggagc gcctgcgggc
      181 cgccggccgg gacgccttgg ccgccggccg ggacgtgttc gaggcggcct ggaagcgggc
      241 ccagccggag gacgccgcca ccctcatcta cacctcgggc acgaccgggc cgcccaaggg
      301 cgtcgtcatc acccaccgca acgcgctctg gacggccgcg tccgtcgagc gcagctggcc
      361 cgagccgatc gcccccggca ccccgtacct ctcctacctg ccgctcgcgc acagctacga
      421 gcggctctgc ggccactacg cctgcctgtg gagcgcccag caggtgcact tctgtcccga
      481 catcctgcgg gtcatggagt acctgcccga ggtccggccg gtcagcttcg tcgccgtccc
      541 ccggctgtgg gagaaggcgt acgcaggcat caccgcggcg atggccgccg agccgaacga
      601 gcgcaagcgc aggctggtca cgcgcgccct cgcggtcggc cgcgaggccg tccgcctcga
      661 gcaggcgggc aagccggtgc ccgccggcct caagctccag cgcaggctgt tcgaccggct
      721 cgtcttctcg aggatccgtg cgaggatcgg cctcgaccgg tgccgggtgg cggtcagcgg
      781 ggcggccccc atcgccgggg aggtcctcga gttcttcctc gccgtgggcc tgcccatggt
      841 cgagggctac gggctgagcg agaacaccgc cggggcgacg gtgaacccgg tcgggcggac
      901 cagggtcggc acggtcggcc tgcccctgcc cggcgtcgag gtccgcctcg acgacgacgg
      961 cgagatcctg gtccggggcg ggaacgtcac cgccggctac ttcaaggagc ccgagaagac
     1021 cgcggagacc ttcgacgccg agggctggct ccacaccggc gacatcggca gcctcgacga
     1081 cgacggcttc atccgcgtcg tcgaccgcaa gaaggagctg atcgtcaccg ccggcggcaa
     1141 gaacatctcg ccggcgaacc tcgagaccct gctgaagcgg cacccgctcg tggggcaggc
     1201 gtgcgtgatc ggcgaccgcc ggccgttcct gtccgcgctg gtcgtgctcg accccgagac
     1261 cgcgcccgcc tgggcgaagc ggcacggcct gcccaccacg cgcatcgagg acctggcggc
     1321 cgaggagcgc gtcgtcgcgg aggtccagcg ggccgtcgac gacgtcaacg agcacgtctc
     1381 ccaggtcgag cgggtgaaga agttcgtcgt cctgccggcc gagtgggcgg tcgacagcga
     1441 ggagctcacc cccaccctga agctcaagcg gcgcgtcatc gtcgagaagt acgccgcgga
     1501 gatcgagtcg ctctacgcct gagcccggct ggatagaggg ggtccggagg ctcgggcagc
     1561 gaagcggtag ccctcggcag gatcagcccc ccggtcaggc aggcatcgac cggaggggct
     1621 gggcggcgac cccccggaac gcgtcgtacg cgaggaccag ggacaccagc ccggcggcgg
     1681 tgtcgaccat gcgggcggcc cgcacgccac cgccgtcgcc ggccggccgg tcggcggcga
     1741 cgagcagcgc ggcggcgatg aacagccccg tccggaacca gggatcccag caggcggcca
     1801 cgtcggacgc tggtggcggc ccggccggga gcgagcccgc gagccggccc aggtcccggg
     1861 cgaccgcctc ggccaccggg tcgccggcac gctcgtccat gaaccagccg agctcgacga
     1921 cgccctcgtg cagcagcacc atgaacgccc cggcggcctg gaagccgttg gcgaccttgc
     1981 tcagggtgac cgagacctgc ccgtacagcc gcgccgtctc gagccgcgag aggttcgcca
     2041 tggtccattc catgacgggc caggcgtccg agatccgcag ggcgctgaat gcctgaccgg
     2101 aagcctgggc gctgcgcagc cgggacaggt tcgcgaatac ccgctcgaac atcacggcgg
     2161 ggtcgatccg ggcgaggtga gcgaatacct ggtcgtacaa ctgcgcggtg tccagccggt
     2221 acaggccgct cgtcagccgg tcgatcgtcg ccgaccggtc ggcaatggcg gcgtgcggtt
     2281 gccgttggcg catcatgtgt ctgctcccga gtggagaccc gagcggtcgc tcgcggtgga
     2341 ccggctcccc ggaggtcggg cggagcatat cttctcagga tttcccgcgg gaattgcccg
     2401 cgggccgcgc gcggcagccg cgaagcggtg gcgcttcgat aacgaagtgg ccacgcagca
     2461 cgacgagcgt ccacatcgac gcggatccgg tcaggaccgg ttgggcgcat tgcgatatcc
     2521 tccgtggata tgatcgattt cgctcgcgtc ctcagggcgg ccggcgtgcc ggtccgggag
     2581 gtggccgggt ggcgccagcg cggccgcccg ccaagcagca accccaggga ccccgcctgg
     2641 ccggccgcca cgaagttcgc cgggatcggc ctgcaccaca ccgcggggca caacgacctc
     2701 agcgtggtgg tgaacggcag gccgggcctc ccggggccgc tcgcgaacct ctacgtcgcc
     2761 agggacggca tggtctacgt cgtcgccgcg gggcgggcca accacctggg ccagggcgcg
     2821 tccgaggtgt accggcggac gctgcgcgac ctcgccccgc tcggggacgc cgccgccctc
     2881 ggcctcgccg actccatggt cggcaacggc gtctacgccg gcatcgaggt ggagaactac
     2941 ggcaccccgg gggacccgta cccgggcgtc cagatcgcgt cgctcgagca ggcgtgcgcg
     3001 gcgctgtgcc ggacggcggg gtggacggcg aaccgttgcg tgcagcaccg ggaatggacc
     3061 cgtcgcaagg tcgacatgag ctaccgcggc cccctgcgtg cggtcgtcgc ggcgatgctc
     3121 tcggaggagg acctcatctt ggacgcgcag accagagcct atttcgacgc gcagttcgag
     3181 ttgctgcggc tcggggacaa ccccgacccg cccaccggcg acacccaccc gcagagcttc
     3241 gagggggtcc tgcgccgcct cgaccggatc gaggcgctac tggagatcgt ctggcgcggc
     3301 gaccagccgg acccggccac cggcgacacc cacccgcaga acctcgagac catctggcgg
     3361 cagggccagg cgacgaccac ggcgctgcag gcccaggcga tcgacgtcac cgccatcagg
     3421 accgacgtcg aggcgatcag gcgggcggtc gaggcgatgc ggccgagcgg cggcgcggcc
     3481 gggcggtgac gggccggcgc gggggcgacg gcgcggagcg cggcaggccg gcggccggag
     3541 ccggcctcga cctcgacggg tccgtcgcgc tcgtgaccgg cggcgccagc ggcatcgggc
     3601 tcgcggtcgt cggccggctc gccgccgccg gcgcgcgggt cgtcgtcgcc gacctcgacg
     3661 aggcgggcgg cgcggccgcc gccgccgcgg cgggcggcgt gttcgtcgcg accgacgccg
     3721 gagacccgga cgcggtgcag gcggcggtcg acgccagcga ggcggcctac ggcgggctcg
     3781 acctcgtcca cctgaacgca gggatcgtca ccggcaacgc cgccctcgac ctcctcgacc
     3841 tcgaccgcta ccgccgcgtc gtcgcggtca acctcgacgg cgtcgtcttc ggggtccgct
     3901 cggcgctgcc cgcgctccgc cgccgcggcg gcggcgccat cgtcgccacc gcgtccctgg
     3961 ccgggctcac cgcctacccc ggcgacccgg tctactcgat gaccaagcac ggcgtcgtcg
     4021 gcctcgtccg caccctcgcc gagccgctcg ccgccgaccg ggtcacgatc aactgcgtct
     4081 gccccggctt cgccgacacg ccgctgctgg accgcttcgc cggccagctg cggactgccg
     4141 ggttcccgct gctctcggcc gacgaggtcg ccgccgccgt gctcaccgcc gccaccggcg
     4201 gcggcacggg ccaggcatgg gtctgccagc ccggccgccc gccggagccg ttccgcttcc
     4261 gcggggtccc cggtccccgg gtggcaggca gcgagggcat ccagccgccg cccaccgagc
     4321 ccggcggccc ctcctgagtg cgggccaggg cgtgcccgcc gccgcgccca ggggtggccg
     4381 cagtctctgc ggccaccgga ctgcggctgg ccacagtgtg tgttcgcggt tgcgtgtggg
     4441 acgatcagcg ccgtgcgtcc aaccgtgctc atcgtcgacg accatgccgc cttccgggcg
     4501 tcggcccggg cgttgctgca ggccgagggc ttcgacgtcg tcggtgaggc ggcagacgcg
     4561 gcgggggcgg tggaggcggt cgccgccctg cggcccgaga tcgtcctgct cgacgttcag
     4621 ctgccggatc tggacggcct ggccgtggcc gaacagcttg ccggggcgga
//
